LOCUS       DBNGTT010000001         3641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1405303, whole genome shotgun sequence.
ACCESSION   DBNGTT010000001 DBNGTT010000000
VERSION     DBNGTT010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1405303"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1210
                     /locus_tag="AC16BC_00005"
     CDS             335..1210
                     /locus_tag="AC16BC_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3240020.1"
                     /translation="MARAGTNAPSGFRVGLHTACALLAFAANSVLCRLALGNGTIDAA
                     SFTAIRLTAGAAALWLLVRVVTPARDTRPRGDWASAAALCLYAVGFSFAYLSLSAGTG
                     ALVLFGAVQSTMILAGLRSGERPHPLQWAGLLAAAGGLVALVFPGLTAPSPAGAALMA
                     VAGVAWGVYSLRGRGATDPIAVTADNFIRSVPLVLLVVLATFRTAHLSPRGVWLAVLS
                     GSLASGIGYVLWYAALRGLTATRAATVQLAVPVLVAFAGVLFLAEAVSPRLIISAILI
                     LGGVGLTLVGRNGAE"
     gene            complement(1319..1981)
                     /locus_tag="AC16BC_00010"
     CDS             complement(1319..1981)
                     /locus_tag="AC16BC_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240021.1"
                     /translation="MKQSCRNARMVILGSVLFSAAALGCAHKITVEIPPRIDLQSYQT
                     IGIVEFSSNSTESLNQAATQEFMSVIQGAQPGVRFLELGPADQVLKAVGRERLDPDAM
                     KALGKKHGVSTIFTGSYDISNVKPKVRLGEDLSSVSASANVRISMVSKQWDAGTGATL
                     WTNSRQGQWPVASVHKDSGSPVSFSMSLPEERYGQFITELVDAVTDDFQPHYERRTVP
                     KS"
     gene            2171..3061
                     /locus_tag="AC16BC_00015"
     CDS             2171..3061
                     /locus_tag="AC16BC_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3240022.1"
                     /translation="MTAATTSHRGRLRTGLYTSLALAAFAANSILCRVALRGDAIDAT
                     SFTAIRLVAGAATLWLLVGLRSGYSRDASPGDWFAALALCVYAVGFSYAYLSLSAGTG
                     ALVLFGGVQLTMLAWGLRSGERPHPLQWIGLFAAASGLVTLVSPGVTAPSPGGAALMA
                     SAGIAWGVYSLRGRGATDPAAATADNFLRAAPLGLLLTLAALPSAHATAQGLGLAVVS
                     GSLTSGIGYVIWYAALRGLTATRAAVVQLAVPVLVAFAGILFLSEPISPRLVISAVMI
                     LGGVALTLIAKDRLVPSAKK"
     gene            complement(3295..>3641)
                     /locus_tag="AC16BC_00020"
     CDS             complement(3295..>3641)
                     /locus_tag="AC16BC_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025063.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HIR3240023.1"
                     /translation="PQGCRKAACPLFFSLFFSYDNASCESFIKTLKREEIYANEYRDL
                     DHLRLNIEVFIDQYYNRQRLHSALGYQAPEEFEGTLASPVTSLGATLRVFRPEERSRR
                     QRAAGQPTSVPT"
BASE COUNT          577 a         1257 c         1105 g          702 t
ORIGIN      
        1 cagcgcacca gaccgaagct tgccacggcg ctttaccgcg cggatgatcc cgatggtctt
       61 tcaccgggat tgaccaaccg gacgttgaca ggagatcgtg gaaacatcta ccctgagccc
      121 cctttgacat gattgagaac aacaagacgt cgcgggatgc cgttggtgcc gggctctgcg
      181 ccgactgccg ctacgcgcag cggatcgaat caatccgagg ctcgatgttc tacttgtgcg
      241 agcgctcggc cggggaccag cggttcaaga aatatccgag cctaccggtc atccagtgtt
      301 ccggccacct ggcgaagccc ccggcataag atccatggcc cgcgccggaa ccaacgcgcc
      361 cagcgggttt cgcgtcggcc tgcatacggc ctgtgcgctc ctggcgttcg ccgccaactc
      421 cgtgctgtgc cgtttggcgc tcggcaacgg gaccatcgat gccgcgagct tcaccgccat
      481 tcgattgacg gcgggcgccg cggccttgtg gctcctggtc cgcgtcgtca cacccgcgcg
      541 ggacactcgc ccgcgcggtg attgggcgtc cgccgccgcg ctgtgtctct acgcggtcgg
      601 attttccttc gcgtatctga gcctgagcgc gggcaccggc gcgctcgtgc tcttcggcgc
      661 agttcagagc acgatgattc tcgcgggcct ccgttctggc gaacgtccgc accccctgca
      721 gtgggccggc ctactcgcgg ccgcgggagg attggtagcg cttgtctttc caggtctcac
      781 cgccccctcg ccggcgggcg ccgcgctcat ggccgtggcc ggcgtggcgt ggggcgtcta
      841 ctccctgcgc ggccgcggcg cgaccgatcc gatcgcggtc accgcggaca atttcatccg
      901 gtcggtgccg ctggtgctgc tcgtagttct agcgacattc cgcacggcgc atctctctcc
      961 acgtggcgtc tggctcgccg tcttatcggg ttccctggcc tccggaatcg gctacgtcct
     1021 ctggtacgcc gcgttgcgag gcctcaccgc cacgcgcgcc gccaccgtgc agttggccgt
     1081 tccggtgctg gtcgcgttcg ccggcgtact cttcttggct gaagcggtct ccccgcgcct
     1141 catcatttcg gccatcctga tcctcggagg cgtcggcctc actctggtcg gacgcaacgg
     1201 cgcagagtag tctgagggga ctcagcgact ctcgaacacg cgcccaaacc gtgcaaccgg
     1261 caaggcgccg cagatggaat cactgccagg ctcgctgttc gagcggtttc cgatcgcgtc
     1321 aggacttcgg caccgttcgt ctctcatagt gcggctggaa atcatcggtc accgcgtcga
     1381 cgagttccgt gatgaattgc ccgtaccgct cctccggaag actcatgctg aatgagacgg
     1441 gagaccctga atccttatga acgctggcga ccggccattg gccctggcgc gaattggtcc
     1501 agagcgtggc tccggtgccc gcatcccact gcttcgaaac catggaaatc cgcacgttcg
     1561 cggacgcgct caccgatgac aagtcttctc ccagcctgac cttgggtttc acgttggaga
     1621 tgtcatagct cccggtgaaa atcgtgctca cgccgtgttt tttcccgagc gccttcatgg
     1681 cgtcggggtc cagcctctcc cgcccgaccg ccttcaacac ctgatccgcg gggcccaatt
     1741 ccagaaaacg cacgccgggc tgggcgccct ggatcacgct catgaattcc tgcgtggccg
     1801 cctgattgag tgactccgtg gagttggacg aaaactccac gatgccgatc gtctgatacg
     1861 actgcaggtc gatgcgcgga ggaatctcca ccgtgatctt gtgcgcgcac cccaacgccg
     1921 ctgcgctgaa caacaccgag cccaagatca ccatccgggc atttcggcag gactgtttca
     1981 tatggcctcc ttgtctccgc gatatacggg cctattctgc gacagcaagt atttccggag
     2041 atgggcaagc gggcaagccc ggcggccagc caagacagcg tggaggctcc tgcgggaggg
     2101 atgccgcgtc cccaggcccc gcgcgaagca ttgactgccg cgtgcggaaa gcgcagactc
     2161 gcgacccgat atgactgccg ccacgacgtc ccaccgcggc cgactccgca caggtctcta
     2221 tacgagcctc gcgctcgccg cattcgcggc gaactccatt ctgtgccgcg tggcgctgcg
     2281 aggcgacgcc atcgacgcca cgagttttac cgcgatccgg ctcgtcgccg gagccgcgac
     2341 cttgtggctg ctggttgggc tccgctccgg atacagccgc gacgcgtctc ccggcgactg
     2401 gttcgccgct ctcgcgttgt gcgtctacgc ggtgggattt tcatacgcgt atctcagcct
     2461 gagcgcaggc accggcgcgc tcgtgctctt cggcggagtg cagctcacga tgctggcttg
     2521 gggtctgcga tccggcgaac gcccccaccc gctccaatgg atcggcttgt tcgcggccgc
     2581 ctcgggtctg gtcacgcttg tatcccccgg cgtcaccgcc ccctcgccgg gcggcgccgc
     2641 gctcatggcc tcggccggca tcgcgtgggg cgtgtactcc ctgcgcggcc gcggcgccac
     2701 cgaccctgcg gccgcgaccg ccgacaactt cctgcgcgcc gcgccgctcg gcctgctcct
     2761 aaccctggcc gcgctcccgt ccgcgcacgc caccgcgcag ggcctgggcc tcgcggtcgt
     2821 gtcgggttca ctgacctccg ggatcggtta cgtgatctgg tacgcggccc tgcgtggcct
     2881 gaccgccacg cgcgccgcgg tcgtgcaact ggccgtccct gtcctggtcg ccttcgcggg
     2941 aatcctcttc ctctccgaac ccatctcccc gcgcctcgtc atctcagccg tgatgattct
     3001 gggcggcgtg gccctaaccc tgatcgcgaa ggaccgatta gtgccctcgg caaagaagta
     3061 aggccaatgg aaaaccgaac ggagtcgtcc acgccatggc ctaggacgct ggcctctcac
     3121 tcggcaggac tcaccctcct ccaactcact gcccctcact aaatccaact ccaaccaaga
     3181 gttgcacgtt ccgccaatct tggcctgagt aggcctgaat aggccagaga gttgtggagg
     3241 tgagcacaat cgcgagcaca atcggctggg gtgaacccct cccgtgagac agttttaggt
     3301 tggcacactc gtcggttgac ccgccgcccg ttgacggcga gaccgttctt ccggtcgaaa
     3361 gacacgcagg gtcgcgccga gtgatgtcac gggcgaggcg agcgtgcctt cgaactcctc
     3421 cggcgcttgg taccccagcg ccgaatgcag ccgttggcga ttatagtact gatcaatgaa
     3481 gacttcgatg ttcaggcgca ggtgatcgag gtcccgatac tcgttggcat agatctcctc
     3541 ccgtttgagc gtcttgataa agctttcaca actagcgttg tcatacgaga aaaaaagcga
     3601 gaaaaaaagg ggacaggctg cttttcggca accttggggc a
//
LOCUS       DBNGTT010000002         3773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_894724, whole genome shotgun sequence.
ACCESSION   DBNGTT010000002 DBNGTT010000000
VERSION     DBNGTT010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_894724"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="AC16BC_00025"
     CDS             <1..856
                     /locus_tag="AC16BC_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR3240024.1"
                     /translation="GPYLFSTHKVRPPYCYRCHLNLAYPACDLACLGEVEQVLRDHHG
                     DIAALIVEPLVYAAAGMITAPPGYLRRLRELCTRYDVLLIADEVATGFGRTGAMFACE
                     REGVTPDLMAVAKGLTGGYLPLAATLTTEKVYDAFLGEYTDFRTFFHGHSYTGNALAC
                     AAALATLDVFVHDRVIEGLAAKAAHLRDALAPLERHPHVGEIRQSGLMVGIELVQDRA
                     TKTRFPVAARIGWTVGREARALGLLTRPLGDVCVLVPPLVSTNEDLSEMARIVEASVH
                     RVLGPPAG"
     gene            complement(886..1470)
                     /locus_tag="AC16BC_00030"
     CDS             complement(886..1470)
                     /locus_tag="AC16BC_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3240025.1"
                     /translation="MMKPFREQNFYELLEISPDASPEHVAKAYALAKRHFGGASLGSY
                     SLFDSAERTEILARLEEAWRTLSDRDRRTRYDRDVLGRATPGDAAASVRSGPAHAPAP
                     TAGPPLTITDVTGSALRARRESIGVPLQEIARNTRISIAYLQFIEEDHIKGLPHDAYL
                     RGYLVQYAKALGLDPHTVADGYIRHAKQLRGHQN"
     gene            complement(1467..2420)
                     /locus_tag="AC16BC_00035"
     CDS             complement(1467..2420)
                     /locus_tag="AC16BC_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR3240026.1"
                     /translation="MPGPEGNGRLVQNEIWAIGGGKGGTGKTFLAASLAIGLARLGKR
                     VIAIDADLGCANLHTALGAESPSATLSDFVKGRVKRLDEVLVDTSLPSLQLISGAHDF
                     LEIANPRHTEKMRFIRQVQDLDVDYIILDLGAGTSFNCLDFFLAADRGILTVIPEPTS
                     IENVYRFIKSAFYRRFKRAVRHPEIKALVAAAMDQKNERGIRTPHDLITGVEAIDPEV
                     GSRLRKTMAEFSPKLVVNQVRSQDDAALGLSMRSSCSKYFGIQVEYLGYVEYDDNVWR
                     AMKRKRPPAVEFPDAASSRGVERVLTNLLKGEQIALDLCVR"
     gene            2531..>3773
                     /gene="glgP"
                     /locus_tag="AC16BC_00040"
     CDS             2531..>3773
                     /gene="glgP"
                     /locus_tag="AC16BC_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR3240027.1"
                     /translation="MSPLDSLINEPKIAYFSMEIGLRTDVPTYSGGLGVLAGDTIKSC
                     ADLKLPVVALTLVCRQGYFRQEMDARGRQIAHPLPWDPAKLMTLVPPRVRVTIDRRPV
                     AVAAWLYWVQSPTGGRIPVLFLDTDLPENAPEHRELTSSLYGGDAAYRLRQEAVLGMG
                     GGKMLQALGVTVRKYHLNEGHSALLTLELLERFRRPIESVWHEDLIWDVERIKDLCVF
                     TTHTPVGAGHDRFPYDLVERVLGAPIPLPVLKRFAGDQELNMTLLALNLSRFVNGVAK
                     KHGEVSRALFPGYEISAITNGVHSFTWTGPDMASVYDAYLPGWANDPELFVRVDHIPD
                     DALWQAHQAAKARLLALVKRATGVEMRPEVLTIGFARRMTQYKRADLVFSDLKRLAQI
                     GGGRLQIVLAGKAHPQDAQGQR"
BASE COUNT          644 a         1270 c         1202 g          657 t
ORIGIN      
        1 cggcccgtac ctcttttcaa cgcacaaggt ccggccgccg tactgttacc gctgtcacct
       61 gaacctcgcc taccccgcgt gcgacctcgc ctgcttgggc gaggtcgaac aggtcctgcg
      121 ggaccatcac ggggacatcg cggcgttgat cgtggaaccc ctggtctacg cggccgctgg
      181 catgatcacg gccccgcccg gctatctccg gcggctgcgc gaactctgca cgcgctacga
      241 cgtcctcctg atcgcggatg aagtcgccac cggattcgga cgaaccggcg cgatgttcgc
      301 ctgtgagcgc gagggcgtca cgcccgatct catggcggtc gcgaaaggtc tgaccggcgg
      361 gtaccttccg cttgccgcca cgctcacgac cgagaaagtc tacgacgcgt ttctcggcga
      421 gtacacggac ttccgaacat tcttccatgg gcacagctac accggcaacg cgttggcgtg
      481 cgccgcggcg ttggccacgc tcgacgtctt tgtccacgac cgcgtgatcg aagggctcgc
      541 cgccaaggcc gcgcatcttc gcgacgcgct cgctccgctg gagcggcacc cgcacgtggg
      601 agagatccgt cagagcggac tcatggtggg catcgaattg gtgcaggacc gcgccaccaa
      661 gacacgattc ccggttgccg cgcgcatcgg atggaccgtc ggtcgcgaag cgcgggcgct
      721 cggcctgctc acccgcccgc tgggcgacgt gtgcgtgctc gtgcccccgc tggtgtcgac
      781 gaacgaggac ctctccgaaa tggcgaggat cgtcgaagca agcgttcacc gggtcctcgg
      841 cccgccggcc ggctgaaccc gacgccggcg cagccggtta ccccgtcagt tctggtggcc
      901 ccgcaactgc ttggcgtgcc ggatgtaccc gtcggccacg gtatgcgggt ccaggccaag
      961 ggctttcgcg tactggacca gatacccgcg aaggtacgcg tcgtggggga ggcccttgat
     1021 gtggtcctct tcgatgaact gcagataggc gatgctgatc cgcgtgttgc gggcgatctc
     1081 ttgcaggggc acgccgatgg actcgcggcg cgcgcgtagc gcggaacccg tcacgtcggt
     1141 gatcgtgagc ggcggaccgg ccgtcggcgc gggcgcgtgt gccggccccg aacggacact
     1201 cgccgccgcg tctccgggtg tcgctcgccc cagcacgtcg cggtcgtagc gcgtgcgccg
     1261 gtcgcggtcc gacaacgtcc gccaggcctc ctccaatcgg gcgagaattt cggtgcgttc
     1321 cgcggaatcg aacagcgagt agctgcccaa ggacgccccg ccgaaatgac gctttgccag
     1381 ggcgtaagct ttggccacgt gttcggggga ggcgtccgga gaaatttcca acagttcgta
     1441 gaagttctgc tcgcgaaacg gcttcatcac cgaacgcaaa gatcgagcgc gatctgttca
     1501 cccttgagga gattggtgag gacccgttca acccctcgcg acgaggcggc atcgggaaat
     1561 tcgactgccg gcgggcgctt gcgcttcatg gcgcgccaga cgttgtcgtc atactcgacg
     1621 tacccgagat actccacctg gatgccgaag tacttcgagc acgagctgcg catggacaat
     1681 cccaacgcgg cgtcatcctg cgagcggact tgattgacca ccagcttcgg cgagaactcc
     1741 gccatggttt tgcgcaaccg actcccgact tccgggtcga tcgcctccac gccggtgatg
     1801 agatcgtgcg gcgtacggat gccccgctca ttcttctgat ccatggcggc cgccaccagc
     1861 gctttgatct cgggatgccg caccgcccgt ttgaatcggc ggtaaaacgc gctcttgatg
     1921 aaccggtaca cgttttcgat cgaggtcggc tccgggatca cggtgagaat gccgcgatcc
     1981 gcggccagga agaaatccag gcaattaaac gaggtgcccg cgcccaaatc caaaatgata
     2041 tagtcgacgt ccagatcctg gacctgacga ataaaccgca ttttctcggt gtgtcgggga
     2101 ttcgcgatct ccaggaaatc gtgggcgccg ctgatcaatt gcaacgacgg caacgaggtg
     2161 tccaccaaca cctcgtcgag ccgcttgacg cggcctttga cgaaatcgga cagggtggcg
     2221 ctgggtgatt cggcgcccag cgcggtgtgc aggttcgcgc atcccaaatc ggcatcgatc
     2281 gcgatgaccc gcttccccag gcgcgcgagg ccgatggcca gactcgcggc cagaaacgtc
     2341 ttgccggttc cgcccttgcc gccgccgatc gcccagatct cgttctgcac caggcggccg
     2401 tttccctctg gtccaggcat gggcctagtt gtaacgtaag ctcgcgccat tttcaagtga
     2461 aaaccgccgc gcgccgaccc gcggaaaacg gcttgcactc gccccgcgat tcgccgtata
     2521 gtgtggcccc gtgtccccgc tcgactccct gatcaacgag cccaagatcg cgtacttttc
     2581 gatggaaatc ggtctgcgga ccgatgtccc gacgtacagc ggcggcttgg gcgtgctcgc
     2641 gggggacacc atcaagagct gcgccgacct caagctcccg gtggtcgccc tcactctggt
     2701 ctgtcgacaa ggatactttc gtcaggagat ggacgcgcgc ggccggcaga tcgcgcaccc
     2761 gttgccttgg gaccccgcga aactcatgac gctggtgccg ccccgcgtcc gcgtgaccat
     2821 cgaccgtcgt ccggtcgccg tcgccgcgtg gttgtattgg gtgcaaagcc ccaccggcgg
     2881 ccgcattccc gtcttgtttc tcgacacgga tctgcccgag aacgcgccgg agcaccgcga
     2941 gctcacgtcg tcgctgtacg gaggagacgc ggcgtatcga ctccggcagg aagcggtcct
     3001 cgggatgggc ggaggcaaga tgctccaggc cctcggcgtc accgtgcgca aataccacct
     3061 caacgaaggg cactccgccc tgctcacgct ggaactgctg gagcggtttc ggcggccgat
     3121 cgagagcgtg tggcatgagg acctgatctg ggacgtggaa cggatcaaag acctttgcgt
     3181 gttcaccacc cacacgccgg tgggcgcggg ccacgaccgg ttcccctacg acctcgtcga
     3241 acgcgtcctg ggcgcgccga tccccttgcc cgtgctgaag cggttcgcgg gcgaccagga
     3301 gttgaacatg acgctcctcg ccctgaacct cagccggttc gtcaacggcg tggccaagaa
     3361 acacggcgaa gtgtcgcggg ccttgttccc cgggtacgag atctccgcga tcaccaacgg
     3421 cgtgcacagt ttcacctgga ccgggccgga tatggcgtcg gtctacgacg cgtacctgcc
     3481 gggatgggcg aatgaccccg aactgttcgt ccgcgtggac cacatcccgg acgacgcgct
     3541 gtggcaagcg caccaggccg ccaaggctcg attgctcgcc ctggtcaaac gcgcgaccgg
     3601 cgtggagatg cggcccgagg tgttgaccat cgggttcgcc cgccgcatga cgcaatacaa
     3661 gcgcgccgac ctggtcttct ccgacctgaa gcgactcgcg cagatcggcg ggggccggct
     3721 ccagatcgtc ctggccggca aggcgcatcc ccaggacgcg caaggccaac gca
//
LOCUS       DBNGTT010000003         3833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1150325, whole genome shotgun sequence.
ACCESSION   DBNGTT010000003 DBNGTT010000000
VERSION     DBNGTT010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1150325"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="AC16BC_00045"
     CDS             complement(<1..1123)
                     /locus_tag="AC16BC_00045"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050355 - inorganic triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HIR3240028.1"
                     /translation="MSAPQRETLEREIKLRVGPGFVLPPLSGRPLHSRVFTSTYYDTN
                     EYRLARHGITLRYRQEGVRGVWQLKLPANEARRELEFSGGRKMPPSAITDLLYAHLRG
                     ESLRPIAKLRTRRTGVRALELDEPIADVVVDSVAVLDGRKMLRRFSEVEIEQVGGHGA
                     AVRRIEKRVRKAGAEDGDPRPKVFQALDLAWPTPPLVAAPGAPPVEHLKALVQLQADR
                     LLQHDPGTRLGTDVEDVHQMRVATRRLRAALRAAGPMLAADWAESLRGELAWLGGALG
                     AVRDLDVLSANLRAECATLRPSERRACARWIDALARERAEKRTELLRALTGERYLALL
                     DRLEAAAASPVVTDAPVGLPDLARKADRALRGAVRARGLA"
     gene            complement(1268..1900)
                     /locus_tag="AC16BC_00050"
     CDS             complement(1268..1900)
                     /locus_tag="AC16BC_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240029.1"
                     /translation="MASNQNSSWLDKVKTGISIIVGLSAVCLIIYNIYSGVRLKKVGV
                     PGIVEFEFGEKAEATGLHDWYGFFGDWDDNRSTYVTREVITLNFENDRRVTGVTKGLV
                     IEADKEVSKTWEFQGFYTADYLVLTYLTKEPIPRGIGSYFLQRSGGDEYVGVLIARDC
                     KNKVILKCPYALSKNDMNVERAREHWPVLFGRQCDVLDLDPSGMIPQCQQ"
     gene            complement(1986..2522)
                     /locus_tag="AC16BC_00055"
     CDS             complement(1986..2522)
                     /locus_tag="AC16BC_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240030.1"
                     /translation="MRTVIFVGLIGLSVIHPALGVPAMAAGALQDAVAPSRSGGTGEV
                     TTKRDQFTGKTAVVLRSMDLGGHVSLFALTSPNGDHVSVSLVVLGQTSEFRGCHPISW
                     KINGQLQSGPPTQHDSQVTDRGVTETIKQEIPVALFRRLSAAQTLMVRLCTRDIEVTG
                     EQVGMLKKFGEAAGIKYR"
     gene            complement(2613..3611)
                     /locus_tag="AC16BC_00060"
     CDS             complement(2613..3611)
                     /locus_tag="AC16BC_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3240031.1"
                     /translation="MTVPPADSHAESGHGHTHGAVDSALLSSRRGIRAVTWSMAGLGA
                     TALAQAVVTAITGSAALLADTIHNLGDAATAIPLWAAFRLGTRPSTKRFTYGYGRVED
                     LAGVGIVVVILASAAAAGYTSIQRLLHPEPVAYLWAVAAAAIIGFAGNEAVALFRIKV
                     GKEIGSAALVADGYHARVDGLTSLAVLLGAIGMWLGYPWADPLIGLLITAAILKIVWD
                     SGKAVLVRLLDGVDSEVVEEIRHAIDHVEGVHDVTEVRVRWLGHRMHAEVNVAVDPDL
                     SVTKGHDIAVNVQHELLHHLRYLASATVHVDPWDTSGEHHHRAVDHAHDDLATHSH"
     gene            complement(3608..>3833)
                     /locus_tag="AC16BC_00065"
     CDS             complement(3608..>3833)
                     /locus_tag="AC16BC_00065"
                     /inference="COORDINATES: protein motif:HMM:NF052847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permuted SH3 domain-containing protein"
                     /protein_id="HIR3240032.1"
                     /translation="AIKAPAGALFREGEQWAVFVLDGNTARKRAVEVGHRNGVEATIA
                     KGLEAGESVIVYPSDAVKNGVKVKQRSGEG"
BASE COUNT          687 a         1296 c         1130 g          720 t
ORIGIN      
        1 ccgccagacc tcgcgcgcga acagccccgc gcaacgcgcg gtcggctttc cgcgcaagat
       61 ccggcaagcc gaccggggca tccgtcacca ccggagaagc ggcggccgcc tccagtcgat
      121 cgaggagcgc cagataccgc tccccggtca acgcgcgcag caactccgtc cgtttctcgg
      181 cgcgttcgcg cgccagggcg tcgatccatc gcgcgcacgc ccggcgctcc gacggccgca
      241 aggtggcgca ttcggcgcgg agattcgcgg acaacacgtc caggtcccgg accgccccca
      301 acgcgccccc gagccacgcc aattcgccgc gcaggctctc ggcccaatcc gcggccagca
      361 tgggaccggc cgcgcgcaat gccgcgcgga gccggcgcgt cgccacccgc atttgatgca
      421 cgtcctcgac gtcggtgccg agtcgagttc ccggatcgtg ttgcaacagc cggtccgcct
      481 gcaactgcac gagcgctttc agatgctcga ccggcggcgc gccgggcgcc gcgacaagcg
      541 gaggagtcgg ccacgccaga tcgagcgcct gaaacacctt gggccgcgga tcgccgtcct
      601 cggccccggc cttgcgcacg cgtttctcga tccgccgaac agccgccccg tggccaccaa
      661 cctgctcaat ctccacctcg gagaaccggc ggagcatctt gcgtccgtcc agcaccgcca
      721 ccgagtccac caccacgtcg gcaatcggtt cgtcgagttc aagcgcccgg acgcccgtgc
      781 gccgggtgcg gagtttggcg atgggcctaa gggattctcc gcgcaggtgc gcgtagagca
      841 ggtcagtgat tgctgacggc ggcattttgc ggccgccgga aaattcgagt tcgcgccgcg
      901 cctcgttcgc gggcagcttg agctgccaga cgccacggac gccctcttgg cgataacgga
      961 gcgtgatccc gtgtcgggcc agccgatact cgttggtgtc gtaatacgtg gacgtgaaga
     1021 cccgcgaatg gagcggacgg cccgacaaag gcggcaggac aaaccccggt ccgacccgga
     1081 gcttgatctc tcgctccaag gtctcgcgtt gcggcgcgga catcgccgtc cgctgtgtcg
     1141 atcccgcctg ggccacggta tttcacagcc tacgatcgcc ggccgaatgg cgtcaatacc
     1201 tcacgcgaaa taaatccgtc cccattagtt cgtccttcgt ccccggacgg attgcactcc
     1261 cctactatta ttgttggcat tggggaatca tccccgatgg atcgagatcg agcacatcac
     1321 attggcgccc aaaaagtacc ggccagtgct ctctggccct ttccacattc atatcattct
     1381 tggatagagc ataagggcac ttgagtatca ctttattttt gcaatctctt gcaatcaaaa
     1441 caccgacgta ctcgtctccg cctgatcgct gcaagaaata gcttcctatg cccctcggta
     1501 ttggttcctt tgtcagatat gtgagtacca ggtagtccgc tgtgtagaat ccctggaact
     1561 cccacgtttt tgatacctct ttatcggcct caattacgag cccttttgta acgcctgtga
     1621 cacggcggtc attttcgaag ttgagcgtta taacttccct tgtgacatac gtcgaccggt
     1681 tatcgtccca gtcgccgaag aagccgtacc agtcatggag tccggtagct tctgcctttt
     1741 cgccaaattc gaactcgacg atgcccggaa caccaacctt cttcaagcgt acgccggagt
     1801 agatattgta aatgatcaaa cagacggcag atagacccac tatgatggag ataccggtct
     1861 tgactttgtc tagccatgac gaattttggt tgctcgccat accgaacctc tccaattatt
     1921 ttatggtgag aggggaaacg acggagggca aatctttacc cacgagtcca tttccccgga
     1981 agcattcatc gatacttgat ccccgccgcc tctccgaatt tcttcagcat tccaacctgc
     2041 tccccggtca cctcgatgtc gcgcgtgcac agccggacca tgagcgtctg cgcggcactg
     2101 agtctccgga acagcgcaac gggaatctcc tgtttgatgg tttcggttac accccgatcc
     2161 gtgacttggc tgtcgtgctg ggtgggaggg ccggactgga gttgcccgtt gatcttccag
     2221 cttatggggt gacagcctcg gaattccgat gtctgaccaa ggaccaccaa tgacacgctg
     2281 acgtggtcgc cattcggact cgtgagtgca aagagcgaga cgtgcccgcc gagatccatg
     2341 cttctgagaa ccaccgcggt tttgcccgtg aattgatcgc gcttggtcgt cacctcgccg
     2401 gtgcctccgc ttctggaggg cgccacggcg tcttgcaagg ctccggcggc catggccgga
     2461 acgccaagcg cgggatgaat cacggatagg ccgatcagcc cgacgaatat gactgtcctc
     2521 atgaggcgcc tctgtgtgcc aacgcgtccg tcaaccggcc atgggatcat atcgaaaatc
     2581 gtgacgtctt tctctatcca gcaaacactg tttcaatgcg agtgcgtcgc gaggtcgtcg
     2641 tgcgcgtgat ccaccgcgcg atgatggtgt tcgcccgagg tatcccacgg gtccacgtgc
     2701 acggtcgcgc tcgcgaggta gcggaggtgg tgcagcagtt cgtgctggac gttgacggcg
     2761 atgtcgtggc ccttggtcac tgagaggtcc gggtccaccg ccacgttcac ctcggcgtgc
     2821 atccggtggc ccagccagcg cacgcgcacc tccgtgacgt cgtgcacgcc ttcgacgtga
     2881 tcaatggcgt gccggatctc ctccacgacc tccgaatcca cgccgtccag cagccgaacc
     2941 agcacggctt tcccggaatc ccacacgatc tttaaaatcg ccgcggtaat cagcaggccg
     3001 atcaacgggt cggcccaggg ataccccagc cacatgccga tggcgcccaa caacaccgcg
     3061 agactggtca gtccgtcgac gcgcgcgtga tacccgtccg cgaccagcgc ggcgctgccg
     3121 atctccttgc cgaccttgat gcggaacagg gcgaccgcct cgttgcccgc gaagccgatg
     3181 atcgcggccg cggcaacggc ccagaggtac gccacgggtt cgggatggag gaggcgctgg
     3241 atcgaggtgt agccggccgc cgcggcgctc gcgaggatga ccacgacaat gcccacgccc
     3301 gcgagatcct cgactcggcc gtaaccgtac gtgaaccgtt tggtggacgg ccgcgtgccc
     3361 aaccgaaacg cggcccataa cggaatcgcg gtcgccgcgt cgcccagatt gtggatggtg
     3421 tcggccagca gcgcggcgct gcccgtgatc gccgtcacca cggcctgagc cagcgcggtg
     3481 gcgccgagac ccgccatgga ccacgtgacg gcccggatcc cgcgccgcga cgacaagagc
     3541 gcggagtcga ccgcgccgtg cgtatggccg tggccggact cggcgtggga atccgcgggc
     3601 ggcacggtca cccttctccg gatcgttgtt tcaccttcac cccgttcttt accgcgtcgc
     3661 tgggatagac gatgaccgac tcgcccgcct ccagcccttt ggcgatcgtg gcctccacgc
     3721 cgtttcggtg cccgacctcc accgcgcgtt tgcgcgccgt gttcccgtcc agcacgaaca
     3781 cggcccattg ctcgccctcg cggaacaagg ctccggcggg cgccttgatg gcc
//
LOCUS       DBNGTT010000004         1892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3322222, whole genome shotgun sequence.
ACCESSION   DBNGTT010000004 DBNGTT010000000
VERSION     DBNGTT010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3322222"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1058
                     /gene="purM"
                     /locus_tag="AC16BC_00070"
     CDS             27..1058
                     /gene="purM"
                     /locus_tag="AC16BC_00070"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3240033.1"
                     /translation="MAKETYRSAGVDIDAGDRTVELIKPLVKATFRPEVLADLGGFAG
                     LFRFDSAKYREPVLVSGTDGVGTKLKIAQLMRRHDTIGIDLVAMCVNDIVVCGAEPLF
                     FLDYFATGKLDPEQARDVIGGIADGCRQAGCALIGGEPAEMPSMYHDGDYDLAGFAVG
                     VVDRPRMIDGHTIAPGDVIVGLASSGLHSNGYSLVRKLLFDVEGLAVDDRLPELNGPL
                     GPVLLTPTRIYAKTVLSLMQRHTLKGIAHITGGGITENLPRVLPPGCAAVIDRSAWTP
                     QPIFQILQSRGEIPDHEMLRTFNMGMGLLLVVAPDASQAVRAAAEALGERAFIVGRIE
                     PGDPSVRYA"
     gene            1051..1686
                     /gene="purN"
                     /locus_tag="AC16BC_00075"
     CDS             1051..1686
                     /gene="purN"
                     /locus_tag="AC16BC_00075"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR3240034.1"
                     /translation="MPKRTVTLGVMASGRGTNLQSILDAIAAGRLDAKIALVLSDRED
                     AQALDRARAAGVPARHLNPKAFPSREAHDAAAADLLRGAGVELVALAGYMRLITRALL
                     DAFPDRIINIHPSLLPAFTGLNAQRQAIEYGVKLAGCTVHFVTEGVDSGAIILQAAVP
                     VRDDDTEATLAERILAEEHRLLPEALQLYAEGRVSIEGRRVRIAPPASVGL"
     gene            1693..>1892
                     /locus_tag="AC16BC_00080"
     CDS             1693..>1892
                     /locus_tag="AC16BC_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240035.1"
                     /translation="MEPRTKEDKLRPEEHLELGILYVREGRFEDAHRSLKQAAHQYGE
                     RDIKAIPPVLLSYYGLCLVALQR"
BASE COUNT          320 a          645 c          634 g          293 t
ORIGIN      
        1 catgacgcgg gatcggacct cctgttgtgg ccaaagaaac ctaccgcagt gccggcgtgg
       61 acatcgacgc cggggaccgc accgtcgagt tgatcaaacc cctggtgaag gccaccttcc
      121 ggcccgaggt gctcgcggat ctgggcggtt tcgccggact gttccggttc gacagcgcga
      181 aataccgcga acccgtactc gtttccggca ccgacggcgt gggcaccaaa ctcaagatcg
      241 cgcaattgat gcgccgccac gacaccatcg gcatcgatct ggtggcgatg tgcgtcaacg
      301 acatcgtggt gtgcggggcc gaacccttgt ttttcctgga ttacttcgcc accggcaaac
      361 tcgatcccga gcaggcgcgg gacgtgatcg gcggcatcgc cgacgggtgc cgacaagccg
      421 gctgcgcgct catcggcggg gaacccgcgg aaatgccctc gatgtaccat gacggggact
      481 acgacctggc cggcttcgcg gtcggcgtgg tcgatcgccc gcgcatgatc gacggacaca
      541 cgatcgcgcc gggcgacgtg atcgtggggc tcgcctcgag cggcctccat agcaacggct
      601 actccctggt ccgcaaactg ctcttcgacg tcgaaggcct ggcggtggac gatcgcctgc
      661 ccgagttgaa cggcccgctg gggccggtgc tgctgacccc gacccgcatc tacgcgaaaa
      721 ccgtgctgag cctgatgcaa cggcacacgc tcaagggcat cgcgcacatc acgggcggcg
      781 ggatcacgga gaacctgccg cgggtgttgc caccagggtg cgccgcggtg atcgatcgga
      841 gcgcgtggac gcctcagccg attttccaga tcctgcagtc gcggggtgag atccccgacc
      901 acgagatgct ccgcaccttc aacatgggaa tggggttgct gctggtggtg gcgcccgacg
      961 cctcgcaggc ggttcgggcc gcggccgagg cgctgggcga acgcgcgttc atcgtcggcc
     1021 ggatcgaacc cggcgacccg tccgtgcgat atgcctaagc ggaccgtcac cctcggcgtc
     1081 atggcctccg gtcgcgggac caacctccag tcgattctgg acgcgatcgc ggccggccgg
     1141 ctcgacgcca aaatcgcgct ggtgctcagc gaccgtgaag acgcccaggc gctggatcgc
     1201 gcccgcgccg cgggggtgcc cgcgcggcac ctgaacccca aggcgtttcc cagccgcgaa
     1261 gcgcacgacg ccgcggcggc cgacctgctg cgcggggcgg gggtggaact ggtcgcgctg
     1321 gccggataca tgcgcctgat cacgcgcgcc ctgctggacg cgtttcccga ccgcatcatc
     1381 aatatccacc cgtcgttgct cccggcgttt acgggcctga acgcccaacg ccaggccatc
     1441 gagtacggcg tgaagctcgc cggctgcacc gtgcacttcg tcaccgaggg cgtggacagc
     1501 ggcgccatca tcctccaggc cgcggtcccg gttcgggacg acgacaccga ggccacgctc
     1561 gccgagcgga tcctcgcgga ggagcaccgc ctgctgccgg aagcgctcca actgtacgcg
     1621 gaggggcggg tcagtatcga aggacgccgg gtccggatcg cgccgcccgc gtcggtgggg
     1681 ttgtaagcgc cgatggagcc gcgaaccaag gaagacaaat tgcgcccgga ggaacacctt
     1741 gaactcggca ttctctatgt cagagaaggg cgattcgagg acgcgcaccg gtcgctcaag
     1801 caagcggccc accaatacgg cgagcgcgac atcaaggcca ttcctcccgt gttgttgtcc
     1861 tactacggcc tctgcctggt ggcactgcaa cg
//
LOCUS       DBNGTT010000005         4240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1406628, whole genome shotgun sequence.
ACCESSION   DBNGTT010000005 DBNGTT010000000
VERSION     DBNGTT010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1406628"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="AC16BC_00085"
     CDS             complement(<1..127)
                     /locus_tag="AC16BC_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3240036.1"
                     /translation="MKVFTIGFTQKSAEQFFGKLRQSGAKRIVDVRLNNVSQLAGF"
     gene            complement(124..756)
                     /locus_tag="AC16BC_00090"
     CDS             complement(124..756)
                     /locus_tag="AC16BC_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3240037.1"
                     /translation="MMRTMSHENPIFTIGHSTHTIDRFIALLHQHGVTAVADVRSSPY
                     SRFNPQFNRETLEKALKEQGIAYVFLGKELGARSADPSCYEKGRVQYDRLARTDLFRE
                     GLERVLKGAKSFRVALMCAEKEPIECHRTLLVSRALVSQGVPVSHILANGLVETHSVV
                     MSRLIGVVGLPKTDLFRSNDELIEAACALQEERIAYANHDMEKPAAEAGR"
     gene            complement(753..1433)
                     /locus_tag="AC16BC_00095"
     CDS             complement(753..1433)
                     /locus_tag="AC16BC_00095"
                     /inference="COORDINATES: protein motif:HMM:NF047206.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual OB domain-containing protein"
                     /protein_id="HIR3240038.1"
                     /translation="MAITKKIVCLANSRKLQGRCIAGREIVGRTPGPWIRPVSDRPHE
                     EVSEYERQYEDGSDPNVLDIIDVPLREPRPAGFQQENWLLDPNEYWVRAGRFLWANLE
                     QLADNSGTLWINGYHTYNGLNDQIPLAQTSTVGSSLKLVRMSALQLRVCRPGEAFGNP
                     KRRVQARFRFAGNDYALWVTAPIIERAYLARANGEYPLGESYLTISLGEPFNDYCYKL
                     VAAVIQQV"
     gene            1608..3092
                     /locus_tag="AC16BC_00100"
     CDS             1608..3092
                     /locus_tag="AC16BC_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR3240039.1"
                     /translation="MPREDGRVLTNDARFLDARVPVPLLATIPLGDLLPVEAYAAVRS
                     ASSFLLESGSRAGRGALGRYSFIGGAPYEILTAQAGALTRWTSLSGETTTTPTTDPLA
                     AAFNRIPRAVVDVPPGAPPFIGGAIGCLGYGLSQRHRRGGLPDRADFFPDLQWMFVDL
                     VGAFDHVERTFTASFCPAPERFAAEPRERLLEEGRARLRDFLTAVTRGLRAGAEPAIR
                     TAPAASIVAGMSRDAYCAMVERAKDYIAAGDIFQANLAQRFTAAHHGSDGLYVYRRLR
                     AINPAPFAAFVDLGETEVICSSPERLVKVSGREVETRPIAGTRPRGATPTDDDDLTAD
                     LLLNPKERAEHLMLVDLERNDLGRVCAYGSVKVDELMVTERYSHVIHIVSNIRGTLRE
                     NASNFDVVDAVFPGGTVTGVPKIRCMEIIDELEPVSRGLYTGSIGYVSWSGHMDWNIV
                     IRTLTRSRGELSFYTGAGIVADSDPDKEYRETLYKAQALIDAVS"
     gene            3251..4114
                     /locus_tag="AC16BC_00105"
     CDS             3251..4114
                     /locus_tag="AC16BC_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3240040.1"
                     /translation="MWAFVDGVFVQDRDAVVSVHDRGFRYGDGVFDTLRVYRGQPFLI
                     ERHLARLIQGATALGIAPLPTASALASTIRDLADRNGTPDALVRTTVTRGVSPGWDVR
                     HAGNPTVVVVEQPFAGYPDQLYVSGASIVILADIRLRPSALTPAIKSLSLLAHVQAKR
                     DATDQGADEALLCTESGFVAEGTVSNVFCVMGGTLRTPPLSDGILQGITREVVLELCR
                     RNSIPAVEDHITPDQCRNAEEVFLTSTGMEVLPVTRVDGHPIGSGQPGAVTLGLRRRY
                     QEFVRETLGTR"
BASE COUNT          779 a         1351 c         1261 g          849 t
ORIGIN      
        1 caaagcccgc caactgggac acgttgttca accgcacgtc cacgatacgt ttagcgcccg
       61 actgacgaag cttgccaaaa aactgctcgg cggatttctg ggtgaagccg atggtaaaga
      121 ctttcatcgc ccggcctccg cagcaggctt ctccatatcg tgattggcat aggcgatccg
      181 ctcttcctgt agcgcgcagg ccgcctcgat caactcatca ttggaacgga ataaatctgt
      241 cttaggcaag cctacgacac cgatcagccg gctcatgacc acggaatgcg tttccaccaa
      301 accgttggcc agaatatggg aaactggtac gccttgggag acgagcgccc ggctgaccag
      361 gagcgtccga tgacactcga tcggctcctt ctcggcacac atgagcgcaa cccggaagga
      421 cttcgcgccc ttcagaaccc gttccaggcc ttctcgaaag agatctgtcc ttgccaggcg
      481 atcgtactgg acacggccct tctcatagca agaggggtcg gccgatctag ccccaagttc
      541 cttacccagg aacacgtagg cgattccctg ttccttcaac gccttttcca gcgtctcacg
      601 gttgaactgc ggattaaaac ggctataggg cgaagaccgg acatccgcga ccgcagtcac
      661 cccatgctgg tgtaaaagag cgataaaccg atcgatcgtg tgagtcgagt ggcctatcgt
      721 gaatatggga ttttcgtgtg acatggttcg catcatactt gttggattac cgctgcaaca
      781 agtttgtagc aataatcatt gaatggttcg ccaagactaa tggtcaagta gctctctcca
      841 agaggatatt cgccatttgc ccgcgccaga taggcccgct caatgatcgg ggccgtgacc
      901 cacagcgcgt agtcgttgcc agcaaaccgg aatcgcgcct ggacacgacg cttgggattg
      961 ccgaacgcct cgccaggcct gcacaccctg agttggagtg cgctcattcg caccagtttg
     1021 agggagctcc caaccgtcga agtctgggcg agaggaattt gatcgtttag tccgttatag
     1081 gtgtggtagc cattgatcca gagtgttccg ctgttgtccg caagttgctc caaattcgcc
     1141 cacaaaaatc gtccggcccg tacccaatac tcattgggat ccagcaacca gttctcctgc
     1201 tggaagcccg cgggccgtgg ctcacgcaat ggcacgtcga taatatccaa aacattcgga
     1261 tcacttccat cttcgtactg ccgctcgtac tctgagactt cttcgtgtgg tcgatcgctc
     1321 accggccgaa tccacggtcc tggcgtgcgg ccgacgatct cgcgccctgc gatgcagcga
     1381 ccctgcaact ttcgtgagtt cgcgaggcag acgatctttt ttgtaatcgc caccagctcc
     1441 tcctcactta cttcgaaaat cgcggcctga gaataatgtc taagacagga gtgagcggaa
     1501 tagggccatc gctctggctc cccaagattt taggtgtttg aacaacgcga tcttagcatc
     1561 tatcgttcgt caatcaaagc cgctaggcct gcttgacact atccggcgtg ccgcgggaag
     1621 atgggcgcgt gttgaccaat gacgcccggt ttctggacgc gcgcgtgccg gttccgctgc
     1681 tggcgacgat tccactcggg gatctcttgc cggtcgaggc gtacgccgcg gtccgctccg
     1741 cgtcgtcgtt tctgttggag agcgggagcc gggccgggcg cggggcattg ggacggtatt
     1801 cctttatcgg cggcgcgccc tatgaaattc tgacggccca agctggcgcg ttgacccgct
     1861 ggacgtccct gtccggcgaa actaccacga caccgacgac cgatcccctc gccgccgcct
     1921 tcaaccgcat tccccgcgcg gttgtggacg tcccgcccgg cgcgccgccg tttatcgggg
     1981 gcgctatcgg atgcttgggg tacggccttt cgcagcggca ccgacgcggc ggcctgcccg
     2041 atcgggccga ctttttcccg gatctccagt ggatgttcgt tgatctcgtg ggcgcgttcg
     2101 atcacgtcga gcgcacgttc accgcgtcgt tctgtccagc gcccgagcga tttgccgctg
     2161 agccgaggga gcgactgctg gaggaaggcc gcgccagatt gcgggacttt ctgaccgcgg
     2221 tgacgcgcgg actccgcgcg ggcgccgagc cggcgatacg gaccgcgccc gcggcatcga
     2281 ttgttgccgg catgtcgcgc gacgcctact gcgcgatggt ggaacgcgcc aaggactata
     2341 tcgcggcggg cgatatcttc caggcgaatc tggcgcagcg attcaccgcg gcgcatcacg
     2401 ggtccgacgg cctgtacgtg tatcggcgcc ttcgtgcgat caaccccgcc ccgttcgcag
     2461 cctttgtgga cctgggcgag accgaggtca tctgctcatc gcccgaacga ctggtcaaag
     2521 tgtcgggccg cgaggtggag accaggccca tcgcgggcac ccggccccgc ggcgccaccc
     2581 cgaccgatga cgacgatctt acggccgatt tgctgctcaa ccccaaggag cgcgcggagc
     2641 acctgatgct ggtggacctg gagcgcaatg acctgggccg ggtgtgcgcg tacggctcgg
     2701 tgaaggtgga cgagctcatg gtcacggagc ggtactcgca cgtcatccac atcgtgtcca
     2761 acatccgcgg cacgctgcgg gagaacgcgt cgaatttcga cgtcgtggac gccgtgttcc
     2821 ccggcggcac cgtgacaggc gtgcccaaga tccgctgcat ggaaatcatc gacgaactgg
     2881 agcccgtgtc ccgcgggctg tacaccgggt ccatcggcta cgtctcgtgg tcgggccaca
     2941 tggattggaa catcgtgatc cgcaccctca cccgctcgcg cggcgagttg tcgttctaca
     3001 ccggcgccgg gatcgtggcg gactctgacc ctgacaagga gtaccgcgaa accttataca
     3061 aagcccaggc gttgatcgac gcggtcagct aggagcctgt ccatgaacgg tcatctgcgg
     3121 cgttgtcgtt tcggaattcc tcctcacgta cgccccagta cgctgcgggg gaattcctcg
     3181 ctccgcctgg catctgacgc gttcctgaac aggctccaca acccgagctc ttagctccac
     3241 tcccaactca atgtgggcgt ttgttgacgg ggtgtttgtt caggaccgcg acgcggtggt
     3301 ctcggttcac gaccgcgggt tccggtacgg ggacggggtg ttcgacacgc tccgggtgta
     3361 ccgcgggcag ccgtttctga tcgaacggca cctcgcgcgc ctgatccaag gcgcgaccgc
     3421 gctcggcatc gccccgttgc ccacggcctc ggcgctcgcg agcacgatcc gcgacctcgc
     3481 ggaccgcaac gggacgcccg acgcgctggt tcgaaccact gtgaccaggg gcgtgtcccc
     3541 gggctgggat gtccgtcacg ccggcaatcc cacggtggtg gtcgtggagc agccgttcgc
     3601 agggtatccg gatcagctgt acgtttcggg cgcctcgatc gtgatcctcg ccgatatccg
     3661 cctccgcccc tcggccctga ccccggccat caaatccctc agcctcctgg cgcacgtcca
     3721 agccaagcgc gacgcgaccg accagggcgc ggatgaagcc ctgttgtgca ccgagtcagg
     3781 cttcgtggcc gaaggcacgg tgagcaatgt cttttgcgtc atgggcggca cacttcgcac
     3841 ccccccgctc tccgacggca tcctgcaggg tattacccgc gaagtggtct tggaactgtg
     3901 tcgccgcaat agcatccccg ccgtcgagga tcacatcacc ccagatcagt gccgaaacgc
     3961 cgaggaagtt tttctgacca gcaccggcat ggaggtcttg ccggtcaccc gagtcgacgg
     4021 gcaccccatc ggatcgggac aacccggagc agtcacgcta ggattgcggc ggcggtatca
     4081 ggaattcgtg agggaaacgc tgggaacgcg gtaaggaatc ttcaggatac ggctgttttt
     4141 tcatagcccg gcactttttc aactttcacc ttcataatcc gccggccttc catatccacg
     4201 atcgtaaact tgagatgatt gtggtgggta atggccccgc
//
LOCUS       DBNGTT010000006         3959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1521, whole genome shotgun sequence.
ACCESSION   DBNGTT010000006 DBNGTT010000000
VERSION     DBNGTT010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1521"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="AC16BC_00110"
     CDS             <1..648
                     /locus_tag="AC16BC_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3240041.1"
                     /translation="CRSGFDRRDAGARTITGGVLVDEEEFRVRGVLLDHKVPCLAFSL
                     EETAHVNVWKTKLREMGFPTGPWLKELKRAVLRGDADETLFRVWWRTKETIEEGMVPL
                     GVLKERALQIVPGQKIAYVVDVAYHEDNARAIVDLARNADTLFIEATFLHHDAERAAD
                     RSHLTARQAGLLARDAGVKVMVPFHFSPRYAGQGDQLRREAEQSFSSPSGSRLEV"
     gene            700..1314
                     /locus_tag="AC16BC_00115"
     CDS             700..1314
                     /locus_tag="AC16BC_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3240042.1"
                     /translation="MRNRVIGGVLLFVVFALSGCGYNTLQTTDEQITASWAEVLNQYQ
                     RRADLVPNLVNVVKGYAAHEEEVLTRVTEARARVGAIQATPGLINDPDAFAKFQAAQG
                     EMTSALSRLLVVAENYPQLKADGLFRDLQAQLEGTENRITVARNRYIKSVQEYNVTVR
                     RFPSNLTAMVFGYHTKPSFTVENEQELAKPPAVDFGTKPQNGPK"
     gene            1419..2261
                     /locus_tag="AC16BC_00120"
     CDS             1419..2261
                     /locus_tag="AC16BC_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR3240043.1"
                     /translation="MRALSWALVLSAALVGLAWAEQPIPPFTAFITDLTNTLSPGQRT
                     TLEQDLSAFSQRKGSQIAVLLVPTTEPEAIEQYALRAAESWKLGRKGVDDGVLFLVAK
                     DDRAMRIEVGYGLEGVIPDAVAKRIISEIVVPYFKQGDFYGGVDAGVERLIRLIDGEP
                     LPPPERRDRQWSGAEDLLPMFIMGALVLGLVLRGLFGRLAGASLTAGIGGLLVWFMLG
                     SLLLGLVAAVIAFFLALVMGTPLGRGGYGGRFGGMLGSGGFGGSFGGGGGGSFGGGGA
                     SGRW"
     gene            2255..2758
                     /locus_tag="AC16BC_00125"
     CDS             2255..2758
                     /locus_tag="AC16BC_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR3240044.1"
                     /translation="MVAYAARVFRHLLNPPWRVRTAFPQRTMRMIEQAIRESEATHNG
                     QIRFAVEAALDIGPLLRNQSARERAIDVFSFLRVWDTEHNNGVLVYLLLADRDVEIVA
                     DRGIARHVAGREWERICRDMEAAFREGRFEDGVIEGVRAVSAHLARHHPGTGGRPNEL
                     PDVPVVL"
     gene            2762..3946
                     /locus_tag="AC16BC_00130"
     CDS             2762..3946
                     /locus_tag="AC16BC_00130"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR3240045.1"
                     /translation="MNAFDVVILGAGAAGLMCAMTAGQRGRRVLVLERANKVGKKIIM
                     SGGGRCNFTNLHVAPDRFLSSNQDFCKSALSRYTQWDFIDLVKRHGIAFHDKGQGQLF
                     CDGSSKAILDMLLTECERAGARIETRYEVEAIDGGSGFQIRTDHGAFKAGALVVATGG
                     LSIPSLGGSGFGYDVAKQFGLRVLPCRPGLVPFTFTDAFRDLSERLSGLSVPARVSTG
                     RHAFTDAILFTHRGLSGPAVLQLSNYWQPGESVVVDLLPERDATELFQSLKLTQPRAM
                     LRTALSQHLPKNLVAELEALWWAEWANRPLTELPKRLLAEIASRLHAWELRPAATEGY
                     RTAEVTVGGVDTRELSSKTMASRSHPGLHFIGEVVDVTGHLGGFNFQWAWSSGRAAGE
                     AV"
BASE COUNT          687 a         1145 c         1416 g          711 t
ORIGIN      
        1 tgccgcagcg ggtttgaccg acgggacgcg ggcgcgagga cgatcacggg cggcgtgttg
       61 gtcgacgagg aggagtttcg cgtgcgcggg gtgttgctcg atcacaaagt cccgtgcctg
      121 gcgttcagcc tcgaagagac ggcgcacgtg aacgtgtgga agaccaagct gagggaaatg
      181 gggttcccga ccgggccgtg gctcaaagag ttaaagcgcg cggtgctgcg aggggatgcc
      241 gatgagacgc tgttccgcgt gtggtggcga accaaggaaa ccatcgagga agggatggtg
      301 ccgttgggag tactcaagga acgcgcgctg caaatcgtac cggggcaaaa gatcgcgtat
      361 gtcgtggacg tcgcgtacca cgaggacaat gcgcgcgcca tcgtggatct ggctcgcaac
      421 gccgacacgc tgttcatcga agccacgttt ctgcaccatg acgccgagcg ggcggccgac
      481 cgctcgcacc tcaccgcgcg ccaggcggga ctgctcgcgc gggacgcggg agtgaaagtc
      541 atggtgccgt tccatttctc gccccgttac gcgggacagg gtgatcagtt gcggcgcgag
      601 gcggaacaat cgttctcgtc gccctccggc tcgcggcttg aagtctgaac ctcagcgtgg
      661 ttgtataggg ccgttgatcg tctgacggtg gagggtgcga tgcggaatcg ggtgatcgga
      721 ggggtgcttc tgttcgtcgt gttcgcgctg agcgggtgcg ggtacaacac gttgcagacc
      781 acggacgagc agatcacggc cagttgggcc gaggtgctca atcagtacca gcgacgggcg
      841 gacctggtgc cgaatctggt gaacgtggtc aaagggtacg cggcgcacga agaagaggtg
      901 ctgacgcggg tgaccgaggc acgcgcccgt gtgggcgcga tccaggccac cccgggattg
      961 atcaacgacc cggacgcgtt cgcgaagttc caggctgctc agggcgagat gacgtccgcg
     1021 ttgtcgcgat tgctggtggt ggctgaaaat tatccgcagc tcaaggccga cggcctgttc
     1081 cgcgatctgc aagcccagct agaaggcacg gagaatcgca ttacggtcgc acgcaaccgc
     1141 tacatcaagt cagtacagga gtacaacgtg acggtgcggc ggttcccgag caatctgacc
     1201 gccatggtgt tcggctacca caccaagccg tcgttcacgg tggagaacga acaagaactc
     1261 gccaaaccgc ccgccgtgga tttcggaacc aagccgcaga acgggccgaa gtgacgagat
     1321 cctcgcggtg aattttgatg agctgggacc tcacgacgcc cagccttaga atcccccctt
     1381 tgaaaaaggg gggaagggag gattttcttc tagtcgctgt gcgtgcgctg agttgggcgc
     1441 tcgttctctc ggcggcgctg gtcggactcg cgtgggccga acaaccgatc ccgccgttta
     1501 ccgcctttat cacggacctc accaacacgc tgagccccgg gcaacggacc acgctggagc
     1561 aggacctttc cgcgttctcg cagcgcaagg gcagccagat cgcggtcctg ctggtgccca
     1621 ccaccgagcc ggaagccatc gaacagtatg cgctgcgcgc ggccgagtcg tggaagctcg
     1681 gacgcaaagg cgtggacgac ggggtgttgt tcctcgtggc caaggacgac cgcgccatgc
     1741 ggatcgaggt tggatacgga ttggaaggcg tgatccccga cgccgtagcg aagcggatta
     1801 tcagcgagat cgtggtgccg tacttcaaac agggagactt ttacggcggg gtggacgccg
     1861 gggtggagcg actgatccgg ctgatcgacg gcgaaccgct gccgccgccg gaacgccgag
     1921 accggcaatg gtcaggcgcc gaggatttgc tgccgatgtt catcatgggc gcgctcgtcc
     1981 tcgggttggt gctgcgcgga ttgttcggcc gattggccgg ggcgtcgttg accgccggca
     2041 tcggcggact gctggtctgg ttcatgctgg gttcgctgct gctcggactc gtggcggccg
     2101 tgatcgcgtt cttcctggcg ctggtgatgg gcacgccgct ggggcgcggc gggtatggcg
     2161 gccgattcgg cggcatgctc ggcagcggcg gattcggagg aagctttggc ggtggcggcg
     2221 gtgggtcgtt cggaggaggg ggcgcgtcgg gacgatggta gcatacgcgg cgagagtgtt
     2281 ccgtcatctc ctcaatcctc cgtggcgcgt gcggactgcg tttccccaac ggacgatgcg
     2341 gatgatcgaa caggcgatcc gcgagagcga ggccacgcac aacggccaga tccggtttgc
     2401 ggtggaggcg gcgctcgaca tcgggccgct gcttcgcaac caatcggcgc gggagcgggc
     2461 gatcgacgtg ttctcgttcc tgcgggtgtg ggacaccgag cacaacaacg gcgtgctggt
     2521 gtacctgctg ttggcggatc gcgacgtgga aatcgtggcg gaccgcggca tcgcgcgcca
     2581 cgtggcgggg cgggagtggg agcgaatctg tcgcgacatg gaggctgcgt ttcgcgaggg
     2641 tcgcttcgag gacggagtga tcgagggcgt ccgggcggtg agcgcgcatc tggcccgaca
     2701 ccatcccgga accggcggac gtccgaacga attgccggac gtcccggtgg tgttgtaatc
     2761 attgaacgcg ttcgacgtcg tgatcctcgg cgccggcgcc gcggggctga tgtgcgccat
     2821 gaccgcgggg caacgcggcc gccgcgtgtt ggtgttggag cgcgccaaca aggtcggcaa
     2881 gaaaatcatc atgtccggtg gcggacggtg caacttcacg aacctgcacg tcgcgccgga
     2941 tcggtttctc tcctccaacc aggacttctg caagtccgcg ctcagccgct acacccaatg
     3001 ggacttcatc gacctggtca aacgccacgg catcgcgttt cacgacaaag gccagggcca
     3061 gttgttttgc gacgggtcgt caaaggccat cctcgacatg ctgttgaccg agtgcgagcg
     3121 ggccggcgcg cgaatcgaga cgcggtacga agttgaggcg atcgacgggg gatccggatt
     3181 ccaaatacgc acggatcatg gcgcgttcaa agcgggcgcg ctggtggtgg ccacgggcgg
     3241 cttgtcgatt ccgagtttgg gcggatcggg gttcggttac gacgtggcca agcagtttgg
     3301 cctacgggtc ctgccctgtc ggcccggcct cgtgccgttc acgttcacgg acgcgtttcg
     3361 ggatctgtcg gagaggttgt ccggactgtc ggtccctgcg cgggtctcga ccggtcgcca
     3421 cgcgttcacg gacgcgatcc tcttcaccca ccgcggtctc agcgggccgg ccgtgcttca
     3481 attgtccaac tattggcagc ccggggaatc cgtcgtggtg gatttgctgc cagaacgaga
     3541 cgccacggaa ctttttcaat ccttgaaact cacacagcct cgggccatgc tgcggaccgc
     3601 cttgagccag cacttgccca agaatctggt cgcggagttg gaagccttgt ggtgggccga
     3661 gtgggcgaat cgcccgctga cggaactccc gaaacggttg ctcgccgaga tcgcgtcgcg
     3721 tctccacgcc tgggagcttc ggccggccgc gaccgaaggg taccgcaccg cggaggtcac
     3781 ggtgggaggg gtcgatacgc gcgagctctc ctcgaaaacc atggcgagcc gttcgcaccc
     3841 cggactccat tttatcggtg aagtggttga cgtgaccgga cacctgggag ggttcaactt
     3901 tcagtgggcc tggtcatcgg ggcgcgcggc gggggaggcg gtctaacgtg ggcgagtcg
//
LOCUS       DBNGTT010000007         4242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_768151, whole genome shotgun sequence.
ACCESSION   DBNGTT010000007 DBNGTT010000000
VERSION     DBNGTT010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_768151"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..528)
                     /gene="gspG"
                     /locus_tag="AC16BC_00135"
     CDS             complement(91..528)
                     /gene="gspG"
                     /locus_tag="AC16BC_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HIR3240046.1"
                     /translation="MTTAPSSHGGFTLIEVMVVVTILAILAILVVPKIVGRTDDARRT
                     AAVVEIKNLEEALHLYKLDSGIYPTTEQGLDALVNKPTSSPVPERWRTGGYIPKVPKD
                     PWNHAYVYLAPGTHGDFDLLSYGGDGERGGEGKDADLENWDLK"
     gene            complement(554..1762)
                     /gene="gspF"
                     /locus_tag="AC16BC_00140"
     CDS             complement(554..1762)
                     /gene="gspF"
                     /locus_tag="AC16BC_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HIR3240047.1"
                     /translation="MPVFEYTALTSEGRTVSGVIDADSPKDARQKLKRSGVFPVDVAA
                     TTARAAAGRPVWGERVSAVETAVLTRQLATLLAAGLPLIEALTALGEQVERAAVKRVV
                     AAVRERIREGGSFAEALAAHPSVFSTLYVNMVRVGEASGTLDRVLTSLAEFLERQAKI
                     RTAVTSALMYPVLMLGVSLSILVFLVAFVVPKVTAVFADLQQALPLPTRILIATSDLV
                     RMGWWVFGLAAVAGGIAFSRYARTATGRRRLDALALRLPIMGRLTRLIALSRFSSTLS
                     TLLAGGVALLTALEIVRHVVRNEVIAEAIDAARERIQQGQSIAEPLRASGVFPPLVTH
                     MIAVGELSGELESMLKKVSEAYDHEVETTLGSITSLLSPVMILVMGGVILFIVLAILL
                     PIFEMSQIVR"
     gene            complement(1822..3600)
                     /gene="gspE"
                     /locus_tag="AC16BC_00145"
     CDS             complement(1822..3600)
                     /gene="gspE"
                     /locus_tag="AC16BC_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HIR3240048.1"
                     /translation="MKPADIVGTPDNASRSPSASKSVSAGPLRLTDILVAKLGVTSEQ
                     IDEALAVQREKGGRIGDILVRLGHVKEEAVLEALAEQLSLPYRRHLSTADIDRGLLVK
                     VPIAFAKRYEILPLREVGGVVHVATAQPLVTAPLDDVRLLLAKPVTPVVVPSRDIVSC
                     INQAYERAGDSAEQVIEDLGAESLDSLATALREEPRDLLEAADEAPIIKLVNSLLFEA
                     AKKRASDIHVEPFERDLLIRYRIDGILYNVLTPPKRIQASIISRIKIMAGLNIAEKRL
                     PQDGRISIKIAGRDVDIRVSAIPTAHGERLVLRLLDKGTLLLQLTEIGLDAERLKTLN
                     RLIRLSHGIVLVTGPTGSGKTTTLYAALNTINAPDKNIITIEDPIEFQLRGVGQMQVN
                     PKINLTFAAGLRSILRQDPDVIMVGEIRDGETAEIAIHASLTGHLVFSTLHTNDAAGA
                     ITRLLDMGIEPFLVASSLVAIVAQRLVRALCPACRHPYHPNGDELAKLGIRSDRTPEP
                     TCFRAVGCAECLSTGYRGRTGIYEILVLDDDLRALILSKADANAIKARAMEHGMRTLR
                     EDGAQKVLSGVTTTEEVLRVTAEDVG"
     gene            complement(3623..>4242)
                     /locus_tag="AC16BC_00150"
     CDS             complement(3623..>4242)
                     /locus_tag="AC16BC_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HIR3240049.1"
                     /translation="VNILSTPQILTSDNQKAEIVVGENRPFPTGQSQTSGGNTITTIE
                     RRDVGITLRLTPQILESDQVKLDVFQEISSVSSESQTAGSVPLGVVTNKRSATTSVLV
                     ADGQTAVIGGLIRDNVITGERKIPLLGDIPVLGWLFKFRSKRTEKTNLLIFLTPTIVK
                     SPTEMAQIRGAKSEAMGRFLSDLDVTKTDLQRGLLDGVNPPDPKP"
BASE COUNT          736 a         1404 c         1353 g          749 t
ORIGIN      
        1 cggcaacacc acgcctcctt cccccctttg aacaagggag gcgagggggg atttcgctgt
       61 tgccggtgtc gaccaagcgt ccctggcgga tcacttcagg tcccaattct ccaaatccgc
      121 gtctttaccc tcaccgcctc gctccccgtc accgccgtag gagaggaggt cgaagtcccc
      181 gtgcgtgccg ggcgccaggt acacgtaggc gtgattccag ggatccttgg gcaccttcgg
      241 aatgtacccg cccgtgcgcc accgctcggg aaccggactc gaggtgggct tgttcaccag
      301 cgcgtccaac ccttgttcgg tggtcgggta gatgccgctg tcgagtttat acaggtgcaa
      361 cgcctcttcg aggtttttga tctccaccac cgcagcggtg cgccgcgcgt catcggtccg
      421 tcccacgatc ttaggcacca cgaggatggc caaaatcgcc aggatcgtga ccacgaccat
      481 gacctcgatc agcgtaaacc cgccgtgcga cgagggcgcg gttgtcattc gtcttgcgta
      541 attcttcaca atatcacctc acaatctggc tcatttcgaa aatcggcagc aggatcgcga
      601 gcacgatgaa caggatcacg ccgcccatga ccaggatcat gaccggggag agcagcgacg
      661 tgatcgaacc cagcgtggtc tcgacctcgt gatcgtacgc ctccgacacc ttcttcaaca
      721 tggattccaa ttccccgctc agttccccca ccgcgatcat gtgggtgacc aagggcggga
      781 agacgccgct ggcgcgcagc ggctcggcga tgctctgccc ctgctggatc cgttcgcggg
      841 ccgcgtcgat ggcctcggca atgacttcat tgcgcaccac gtgcctgacg atttccagag
      901 ccgtgagcag cgccacgccg ccggccagca acgtgctcag ggtcgaggaa aagcgcgaga
      961 gcgcgatgag ccgggtcagc cgccccatga tcggaagccg caacgccagg gcatcgaggc
     1021 gacgacggcc cgtggccgtg cgggcgtagc gcgaaaacgc gatgccgccc gccaccgcgg
     1081 ccagaccgaa cacccaccat cccattcgca ccaggtcgct ggtcgcgata aggatgcgcg
     1141 tgggcaacgg caacgcttgt tgcaggtccg caaacacggc cgtgaccttc ggcaccacga
     1201 acgcgacgag aaacaccaga atcgacaggc ttacgcccaa catcaacacc ggatacatca
     1261 acgcgctcgt gaccgcggtg cggatcttgg cctgtcgttc gagaaactcc gccaaactcg
     1321 tcaggacccg atcgagcgtg ccgctggctt cgccgacacg caccatgttg acgtagagcg
     1381 tggagaagac cgagggatga gcggccagcg cctcggcgaa ggaccctccc tcgcggatcc
     1441 gctcgcgcac cgcggccacc acccgcttga ccgcggcgcg ctcgacctgc tcgccgagcg
     1501 cggtcaacgc ctcgatgagc ggcaaccccg cggcgagcag cgtggcgagc tgacgcgtga
     1561 gcaccgcggt ctcgaccgcg gacacgcgtt cgccccacac cggccgaccg gccgcggccc
     1621 gcgccgtggt cgcggccacg tccacgggga acacgccgga gcgcttgagt ttctgtcgag
     1681 cgtccttcgg gctatcggcg tcgattaccc ccgacaccgt gcggccctcg gacgtcaacg
     1741 ccgtgtactc aaagacgggc atgggaggat ggtcgcccgg tcaggaaaac atgcgcggtc
     1801 gttgatcggt gaacgcgatc gtcatcccac gtcctcggcg gtcacgcgca acacttcttc
     1861 cgtggtcgtg accccggaca gcactttctg cgcgccgtct tcgcggagcg tccgcatgcc
     1921 gtgctccatg gcgcgggctt tgatggcatt ggcgtccgcc ttggacagaa tcagggcccg
     1981 caagtcatcg tccagcacga ggatctcgta gatcccggtc cgaccgcggt accccgtgga
     2041 gaggcattcg gcgcacccca cggcccgaaa acacgtgggc tccggcgtcc gatccgaccg
     2101 aatgccgagt ttggcgagtt cgtcgccgtt cgggtgatac gggtggcggc acgccggaca
     2161 gagcgcgcgc accaggcgct gcgcgacgat cgccaccagg gaggacgcca ccaagaacgg
     2221 ctcgatgccc atgtccagca gccgcgtgat ggcgcccgcc gcgtcgttgg tatggagggt
     2281 cgaaaacacc aagtgcccgg tcagcgacgc gtggatcgcg atctccgcgg tttctccgtc
     2341 ccggatctcg ccgaccatga tcacgtccgg gtcctgacgg aggatcgagc gcaggcccgc
     2401 ggcgaacgtg aggttgatct tcggattgac ctgcatctgc ccgaccccgc ggagctggaa
     2461 ctcgatgggg tcttcgatcg tgatgatgtt cttgtccggg gcgttgatcg tgttgagcgc
     2521 ggcgtacaac gtggtggtct tgccgcttcc ggtcggaccg gtcacaagca cgatgccgtg
     2581 gctcaggcga atgagacggt tcaaggtttt aaggcgctcc gcgtcgagcc cgatctcggt
     2641 caactgcagc aggagcgtgc ccttgtccag cagacggagc accagccgct cgccatgcgc
     2701 ggtggggatg gccgacacgc gaatgtccac gtcgcggccc gcgatcttga tgctgatgcg
     2761 cccgtcctgc ggcaggcgct tctccgcgat gttgagcccc gccatgatct tgatgcgcga
     2821 gatgatgctg gcctggatcc gcttgggcgg ggtcaacacg ttgtacagaa taccgtcgat
     2881 gcgataccga atgagcaggt cccgctcgaa cggctcgacg tggatgtcgc tcgcccgctt
     2941 cttggcggcc tcgaacagga gggagttcac gagcttgatg atcggcgcct cgtcggccgc
     3001 ctcgagcaga tcgcgcggct cctcccgcag cgccgtggcc aacgaatcca gggattcggc
     3061 gccgaggtct tcgatcacct gttcggccga atctccggct cgctcgtacg cctggttgat
     3121 gcacgacacg atgtcgcggg acggcaccac caccggcgtg accggcttgg ccaacaagag
     3181 ccggacatcg tccaacggcg ccgtgaccaa cggttgggcc gtggccacgt ggaccacgcc
     3241 cccgacctcg cgcagcggaa ggatctcgta ccgcttggcg aacgcgatcg ggactttgac
     3301 gagcaggccg cgatcaatgt cggccgtgga gaggtgccgt cggtacggta ggctcagttg
     3361 ctcggccaag gcttcgagca ccgcctcctc cttgacgtgg cccagacgca cgagaatgtc
     3421 cccgatgcgc ccgccctttt cgcgctgaac cgccagcgct tcgtcgatct gttcggaggt
     3481 gacgccgagt ttggcgacca gaatgtcggt caaccgcagg gggccggcgc tcacgctctt
     3541 ggaggcgctg ggagaacggg acgcgttatc cggcgtcccg acgatgtcgg ccggtttcat
     3601 gacagtcgcg cgcccatgtg cgctacggtt tggggtccgg agggttgacg ccgtccagca
     3661 acccgcgctg gagatcggtc ttggtcacgt cgaggtcgct cagaaagcgc cccatcgcct
     3721 cgctcttggc gccgcggatc tgcgccatct cggtcggact cttgacgatc gtgggcgtga
     3781 ggaaaatgag gaggttggtc ttctccgtcc gcttgctgcg aaacttaaag agccatccca
     3841 gcaccggaat atcgcccagc agcgggatct tgcgctcgcc cgtgatcacg ttgtcgcgaa
     3901 tcaatccgcc gatcacggcg gtctgcccgt cggccacgag cacggaggtc gtggcggacc
     3961 gtttgttggt caccacgccc agcgggacgg acccggcggt ctgcgactcg ctggacacgc
     4021 tggagatttc ctgaaacacg tccagcttga cctggtcgct ttccaagatc tgaggggtta
     4081 gacgcaaggt gatccccacg tcgcgccgct caatcgttgt gatcgtattg ccgccggagg
     4141 tctggctctg accggtcggg aacgggcgat tttcacccac cacgatctcg gccttctggt
     4201 tgtcgctggt caagatctgc ggtgtggaga ggatattcac gt
//
LOCUS       DBNGTT010000008         2767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_512851, whole genome shotgun sequence.
ACCESSION   DBNGTT010000008 DBNGTT010000000
VERSION     DBNGTT010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_512851"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..832
                     /locus_tag="AC16BC_00155"
                     /pseudo
     CDS             263..832
                     /locus_tag="AC16BC_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017009.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            912..1760
                     /locus_tag="AC16BC_00160"
     CDS             912..1760
                     /locus_tag="AC16BC_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3240050.1"
                     /translation="MVRILDRANVLIGLKELGLSSDNLERLDQLIHQPYGIVLVTGPT
                     GSGKSATLYAALNAIKSPEVNIVTIEDPIEYQIAGINQVQINPKRELAFASVLRSVLR
                     QDPDIIMVGEIRDPETGAMGTEAALTGHLVFSTLHTNDAGGAVTRLIEMGIEPFLLAP
                     SILGVVAQRLVRKVCVQCRESYEPRPAELERLGLDGLQGDRQFARAKGCAHCQHRGYR
                     GRTAIHEILIIDEIMRQMISEKVAQATLMDYAPQRGFIDMRIDGLKKMVAGLITVEEV
                     LRVSKG"
     gene            1767..2150
                     /locus_tag="AC16BC_00165"
     CDS             1767..2150
                     /locus_tag="AC16BC_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3240051.1"
                     /translation="MGQTPKTVLVVDDSSFMRTSLKAILEKLGYTVVGMDENGIEAVA
                     KFRDLKPDVVTLDIIMPQMGGLQGLKLLKSVNPNATVVMVSTMADRESVTECVKAGAA
                     HYLLKPFDQTKVAEVMKQVTNARAA"
     gene            complement(2208..>2767)
                     /locus_tag="AC16BC_00170"
     CDS             complement(2208..>2767)
                     /locus_tag="AC16BC_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HIR3240052.1"
                     /translation="QTTAEMIFAAGDVCSRFQFTHAADAHAQIVIQNALFPHPFGLGM
                     ARVSSLVIPRVTYTQPEVAHVGMTDAEARAAGIEGETFAFKLDEIDRAILDGDEEGFA
                     RVRVKRGTGTILGATIVSTRAGDLISEATVVMHNGLGLSAIAGTIHPYPTQAEVLKRA
                     ANVWRKSTFTDKKQRILKRWFEWAR"
BASE COUNT          556 a          905 c          797 g          509 t
ORIGIN      
        1 cgcacgcgtc tcttccagtc ggcgttcttc cgccgtctga ggcaaatcgt ctcgccgagt
       61 tttgatgggg ttcgagtcca cagcatccct gctcgtgtct cttcaggtcc ctatatagta
      121 tccgcagatc gatgagagtc aacgagcccg cctcgtctcg ccgaatcgat cccatacggg
      181 agggcatccg ctcggtcgat caggcctggc catgatcgaa tggtctgcta tgcttgacgg
      241 ctgagatcgc ccgtaagcat ccattacatg cgggcgatcc cggccaacgg tcaccgggga
      301 tgcgctccgg actatcccgg aatcgctcgc gcgcaagctg tgtgtggtcc caatcttctt
      361 ggtcgcgaag gaattgaccg tggcgatcgc ggacccgacg gaaatcgata cgatcgactt
      421 tctcgcgcgg gaaactcacc acgtgatcca acccgtgctc gccccacgcc gtcagatcac
      481 ggcggcccac gatcggttct acgccgagga gtccgcgctc tcatcgctca acaccgagga
      541 cctccaacgg ttcgacgtcg accacggtgg tcccggcgag gtcgagcggc tcaaagcggt
      601 gggcaaagaa attccgatcg taaagatcgt ggatcaaatc ttcgcccgcg cgatcgccga
      661 aggccccagc gacatccaca tcgagcccgg cgaaaccaaa gtccaggtac ggttccgcgt
      721 cgacggcctc ctgcgcgaag tcacgtcgtt ctcgccgctt ctgcgcgccg cgatcacgtc
      781 gaggatcaag atcctttcca atctcgatat cgcggaacgg cagaagcccc aggacgggag
      841 gatccatctg tctgcgctcg atcgtgatct ccgcgtgtcc accctcccga ctccaccacg
      901 gcgagaaggt cgtggtccgc atcctcgacc gcgccaacgt gctgatcggc ctgaaggagt
      961 tgggcctgag ctcggacaac ctcgaacgcc tcgaccaatt gatccatcaa ccctacggca
     1021 tcgtcctggt gaccgggccc accggcagcg ggaaaagcgc cacgctctat gcggccctca
     1081 acgcgatcaa atcgcccgag gtcaacatcg tcaccatcga ggatcctatc gaataccaaa
     1141 tcgcaggaat caaccaggtc cagatcaacc ccaagcggga attggcgttc gccagcgtat
     1201 tacgttcggt gctgcggcag gaccccgaca tcatcatggt gggcgaaatc cgggacccgg
     1261 aaaccggagc gatgggcacc gaggcggcgc tcaccggcca tctcgtgttt tccacgctcc
     1321 acaccaacga cgccggaggg gcggtgaccc gtttgatcga aatgggcatc gagccgtttc
     1381 tgctcgcgcc ctcgattctc ggcgtggtag cgcagcgatt ggtccgcaag gtctgtgtgc
     1441 agtgccgcga atcgtacgaa ccgcggcccg ccgaactcga acggctcggg ttggacggac
     1501 tccagggcga ccggcagttt gcccgcgcga agggatgcgc acattgccaa caccggggct
     1561 atcgcggccg cactgcgatc cacgaaatcc tgatcatcga cgagatcatg cggcagatga
     1621 tcagcgaaaa ggtcgcgcag gccacgctca tggattatgc cccgcagcgc ggctttatcg
     1681 acatgcgcat tgacggcctg aagaaaatgg tcgcgggact catcaccgtg gaggaagtcc
     1741 tccgcgtcag caagggataa ggagccatgg gacaaacgcc gaaaacagta ctcgttgttg
     1801 acgattccag ctttatgaga acctccttaa aagccatttt ggagaagctg ggctacaccg
     1861 tggttggcat ggatgaaaac ggcatcgagg cggtcgccaa gttccgcgat ctcaagcccg
     1921 atgtggtgac gctcgacatc atcatgcccc agatgggcgg tctgcaaggg ctcaagctgc
     1981 tcaaatcagt caatcccaac gccacggtcg tcatggtgtc aacgatggcc gaccgtgagt
     2041 cggtcacgga gtgcgtcaag gctggagccg cgcactacct cctgaagccc ttcgatcaga
     2101 ccaaggtcgc tgaggtcatg aagcaggtca cgaacgcgcg cgccgcttaa cccgcatcga
     2161 accgacacgc gggctccggg gccgcaacgt cgcctccccg tcacccgtca tcgagcccat
     2221 tcgaaccatc gtttcaagat ccgctgtttc ttgtcggtaa acgttgattt tctccaaacg
     2281 ttggccgctc gcttgagcac ctcggcctgc gtgggatacg gatggatcgt gcccgcgatc
     2341 gccgagagcc ccagcccgtt gtgcatgacg acggtcgcct cgctgatgag atcacccgcg
     2401 cgcgtggaca cgatggtcgc gcccagaata gttccggtcc ccctcttgac gcgcacgcgc
     2461 gcgaaccctt cctcgtcgcc gtcgagaatc gcgcgatcga tctcatccag cttgaacgca
     2521 aatgtctccc cctcgattcc cgcggcccgc gcctcggcgt cggtcatccc cacgtgtgcc
     2581 acctccggct gcgtgtaggt gacgcgtgga atcaccagcg aactcaccct ggccatcccc
     2641 agcccgaatg gatgcggaaa cagggcgttt tgaatcacga tctgcgcgtg ggcgtcggcg
     2701 gcatgggtaa actgaaaccg cgagcacacg tctcccgcgg caaagatcat ttccgccgtc
     2761 gtctgta
//
LOCUS       DBNGTT010000009         2980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2046061, whole genome shotgun sequence.
ACCESSION   DBNGTT010000009 DBNGTT010000000
VERSION     DBNGTT010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2046061"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="AC16BC_00175"
     CDS             <1..656
                     /locus_tag="AC16BC_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR3240053.1"
                     /translation="DFPLTIDDVVTGGKRSVRLSVGEACATCGGSGRVSQVAAGGGRR
                     RTVATVCPTCHGHGQVSRTQTIEVTIPKGLKDGSKIRLAGLGGRGAHGGRPGDLYLRV
                     KLQPHPTFRAEGYDLFGQLPVWDDEAVLGADVSVPTPSGSVMVRVPPNSQSGRKLRLK
                     GKGLPKAAGEGAGDLYWELQVVTPADASTDEREHYEALRRLRSARNGRDRIRKRLAS"
     gene            663..1853
                     /locus_tag="AC16BC_00180"
     CDS             663..1853
                     /locus_tag="AC16BC_00180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3240054.1"
                     /translation="MIAVLALFLLIGHGFHLLGHYRRCLDGYVRLAATASSIRSNAVS
                     VIIPARNEARDLGKTIESLQRQQGVTLEVLIVDDHSMDETATIAQRHAALDSRVRALS
                     APTLPDGWCGKPHAQAYGASIATHPLLLFADADVRFAPAALASAVEELERRRVDLVTL
                     IPATELGSFWERAVQPVMAAVIFYGVSFTRVNDPADPQSMGVGAFLLVRRSTYDAAGG
                     HQAIRDRIVDDYALADSVKRAGGKLWLADGGDLLSIRMYHSFAELWRGWSKNLYEGLR
                     LPVRFRMGRRERVIVNDRPLVVLGLLCGFLVSTFILPIAMPIWVTLTPTHPAVSLTTY
                     GVSAVFVVAWTSLCRRLGMGRVWAVALPLGALVVAAIALHSTWRARLGGGLLWRGRQY
                     RARR"
     gene            1900..>2980
                     /locus_tag="AC16BC_00185"
     CDS             1900..>2980
                     /locus_tag="AC16BC_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3240055.1"
                     /translation="MLTLHEIRKAYGSRVLFDGASLQVGARERVALVGPNGAGKTTLF
                     EMIAGRLSPDSGQVQTQKGVVIGYLPQEIIELKGKTILQEVLSAAAGATSMEHRMRVL
                     QQEMAKASGDELEALLTEYGELQHRYEAVGGYNIEAQAREILSGLGFRAEAFEKPTDH
                     LSGGWLMRVALAKLLLQNPDLLLLDEPTNHLDLESVIWLEGFLGEYDGSVVLISHDRE
                     FMNHLVSRVVEVEQGQLLSYPGTYDEYVEARAAAREILMASYANQQKRIQEIQQFVER
                     FRYKATKARQVQSRVKMLDKLDRIKIPPERKRVRFGFPQPPRSGREVAVLEGVRKAYP
                     GRVIYDRLDLIVTRGQRVALVGPNGA"
BASE COUNT          475 a          929 c         1053 g          523 t
ORIGIN      
        1 ccgatttccc gttgacgatc gacgacgtgg tgacgggcgg caaacgcagc gtgcggttgt
       61 cggtcggcga ggcgtgcgcc acgtgcggcg ggtccggccg tgtgtcgcaa gtggcggccg
      121 gcggcggccg gcgcaggacc gtggcgaccg tttgccccac gtgtcacggc cacgggcagg
      181 tgtcgaggac ccagaccatc gaggtcacga ttcccaaggg gctgaaggac gggtccaaga
      241 ttcgtctggc cggcctgggc ggacgcggcg cgcacggcgg gcggcccggc gacttgtact
      301 tgcgggtcaa gctccagccg catccgacct ttcgtgccga gggctacgac ctcttcggtc
      361 agctcccggt gtgggacgac gaagccgtgt tgggcgcgga cgtctccgtg cccacgccgt
      421 ccgggtccgt gatggtgcgc gtgccgccca attctcagag cgggcgcaaa ctgcgcctca
      481 agggcaaagg actgcccaag gcagcgggcg aaggcgcggg tgatctgtac tgggaactgc
      541 aggtcgtgac ccccgccgac gcttccacgg acgagcgcga gcattacgaa gcgctgcggc
      601 gactccgaag cgcgcgcaac ggacgcgatc ggatccgcaa gcgcctcgcg tcgtagtcgc
      661 gcgtgatcgc cgtcctcgcg ctgttcctcc tgattgggca cggattccac ctcctcggcc
      721 actaccgtcg gtgtctcgat gggtacgttc gcctggcggc cacggcgtcc tcgatccgct
      781 ccaacgccgt gtcggtgatc attccggcgc gcaatgaggc gcgggatctg ggcaagacga
      841 tcgaatcgtt acagcggcaa cagggggtca cgttggaggt gctgatcgtc gacgatcact
      901 ccatggacga gaccgcgacg atcgcgcagc ggcacgccgc cctcgattcg agggtgcgcg
      961 cgttgtcggc gccgacgctt cccgacggtt ggtgcggcaa gccccacgct caagcgtacg
     1021 gcgcgtccat cgcgacccac ccgttgctgc tcttcgccga cgcggacgtc cgtttcgccc
     1081 cagccgcgct ggcgtcggcc gtcgaagaac tcgaacgccg tcgggtggac ctcgtgacgt
     1141 tgatcccggc cacggaactg gggagctttt gggaacgcgc ggtgcaaccc gtgatggcgg
     1201 ccgtgatctt ttacggcgtc agttttaccc gggtgaacga ccccgctgat ccgcagagca
     1261 tgggcgtcgg cgcgttcctt ctggtgcgcc gatcaacgta cgacgcggcg ggcggccacc
     1321 aagccattcg cgaccggatc gtggacgatt acgcgctcgc cgactccgtc aaacgcgcgg
     1381 gcgggaagct ctggctcgcg gacggcggcg atctgttgtc gatccggatg taccactcgt
     1441 tcgcggagct gtggcgcggc tggtccaaaa acctgtacga aggtttgcgc ctgcccgtgc
     1501 ggtttcgcat gggacggagg gaacgggtca tcgtgaacga tcgacccttg gtggtgttgg
     1561 ggctcttgtg cggcttcctc gtctccacat tcatcctgcc gatcgcgatg ccgatctggg
     1621 tcacgctgac gccgactcat ccggccgtct cgctgacgac gtatggcgtg tcggcggtgt
     1681 tcgtcgtggc ctggacgtca ttgtgccgca ggctcgggat ggggcgggta tgggcggtcg
     1741 ccttgccgct gggcgcgctg gtggtcgcgg ccatcgcgct gcacagcacc tggcgcgcgc
     1801 gcctgggtgg tggcttgctg tggcggggcc gccaataccg cgcgaggcga tgacccggcg
     1861 cttgcgggcg ccctgaccgg ttccgtatac tcgcatcgta tgttgactct ccatgagatc
     1921 cgcaaagcct atgggagccg cgtgctcttt gacggcgcgt cgctgcaagt gggcgcccgt
     1981 gagcgcgtgg ccctggttgg ccccaacggc gcgggcaaaa ccaccttgtt cgagatgatc
     2041 gcgggccgcc tctcgccgga cagcgggcag gtgcagaccc agaagggcgt ggtcatcggg
     2101 tatttgccgc aggagattat cgagctcaaa ggcaagacga tccttcagga agtgctctcg
     2161 gccgcggccg gagccaccag catggagcac cggatgcggg tgctgcaaca ggaaatggcc
     2221 aaggcgtccg gcgacgagct cgaagccctg ctgaccgaat acggcgagct gcagcaccgg
     2281 tacgaggccg tgggggggta caacatcgaa gcacaggcgc gagaaatttt gagcggtctc
     2341 gggtttcgcg cggaggcgtt cgagaaaccg accgaccacc tgtccggcgg ctggttgatg
     2401 cgcgtggcgt tggccaaact gctgctgcag aatccggatc tcttgttgct cgacgagccc
     2461 accaatcacc tggacctgga gtcggtcatc tggctggaag gtttcttggg cgagtacgac
     2521 gggtcggtgg tgcttatttc gcacgatcgg gaattcatga atcacctggt gtcgcgggtg
     2581 gtggaagtcg aacagggcca gttgctctcc tatccgggca cgtatgatga gtacgtcgag
     2641 gcccgcgccg cggcgcgcga gatcctgatg gcgtcgtacg ccaaccagca aaagcgcatt
     2701 caggaaatcc aacagttcgt cgaacggttc cggtacaagg cgaccaaggc ccgtcaggtg
     2761 caaagccggg tcaagatgct ggataagctg gaccggatca agatcccgcc ggagcgaaag
     2821 cgcgtccggt tcggattccc tcagccgccg cgaagcggcc gggaggtggc ggtgctcgaa
     2881 ggcgtccgca aggcctaccc ggggcgcgtg atctacgacc gattggatct gatcgtcacg
     2941 cgcggccagc gggtcgcgct cgtgggtccc aacggcgcgg
//
LOCUS       DBNGTT010000010         1901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2046406, whole genome shotgun sequence.
ACCESSION   DBNGTT010000010 DBNGTT010000000
VERSION     DBNGTT010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2046406"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="AC16BC_00190"
     CDS             <1..882
                     /locus_tag="AC16BC_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR3240056.1"
                     /translation="REMVESGVIAPVADADAASLPKLPKSVDQAIKSGDVMVEPLIKT
                     TISDDRGDEPCYDGYPASELIKKGYQIPHVIGLLWNKKLMSAQEAEIIKRIIMLSADH
                     GPCVSGALVTIIAAGAGINMAQAVAAGMMMIGPRFGGAVTDAGKWFKYAIDQKLTPEA
                     FLDYMKANVGPVPGIGHRVKSVKNPDKRVAELVSYVKSLSIPTPHLDFALQVEKITTA
                     KKDTLILNVDGTIAAILVDLGYPVESLNGFFILSRTIGFIGHWVDQKRQGSRLIRLFD
                     YLVNYATPKRREVPPLG"
     gene            1095..1808
                     /locus_tag="AC16BC_00195"
     CDS             1095..1808
                     /locus_tag="AC16BC_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3240057.1"
                     /translation="MNESNTIQSPQESGRMPKFVTVEVMGKKHQVPEGVTVIQALWYT
                     GHEMIRGIGCLGGTCGACGMVYRMPGQYELLNGLACQTTVRDGMWFSMVANYPAAQAH
                     YRIPELTNAKEQLFEMYPESARCVNCNACNRVCPQGIDVRSSIWYAVFGDFQKVADLT
                     MSCNNCGLCITRCVADMPPNLVGLYARRTYGALYAPAAEELARRVSDIGDGKYRDELD
                     RLLSADSAELKSRAAAVGR"
BASE COUNT          337 a          587 c          657 g          320 t
ORIGIN      
        1 cgcgagatgg tcgagtccgg cgtgatcgcg ccggtggcgg acgcggacgc ggcctcgttg
       61 cccaaactgc ccaagagcgt ggatcaggcg atcaagagcg gcgacgtgat ggtcgagccc
      121 ttgatcaaga ccacgatttc ggacgaccgc ggcgacgagc cctgttacga cgggtacccc
      181 gcgtcggaat tgatcaagaa gggctaccag atcccgcacg tgatcggcct gttgtggaac
      241 aagaagctca tgtccgcgca ggaagccgag atcatcaaac gcatcatcat gttgtcggcc
      301 gaccacgggc cgtgcgtgtc gggcgcgttg gtcacgatca tcgcggcggg cgcgggcatc
      361 aacatggcgc aggccgtggc cgcgggcatg atgatgatcg ggccgcgctt cggcggcgcg
      421 gtcacggacg cgggcaagtg gttcaagtac gcgatcgacc agaaactgac ccccgaggcg
      481 ttcctggact acatgaaggc gaacgtgggg ccggtgccgg gcatcggcca ccgcgtcaag
      541 agcgtgaaga acccggacaa gcgcgtggcc gaactggtct cgtacgtgaa gagcctgagc
      601 attcccacgc cgcacctgga ctttgcgctg caggtcgaaa agatcaccac cgcgaagaag
      661 gacacgctga tcctgaacgt ggacggcacc atcgccgcga tcctggtcga cttggggtat
      721 ccggtcgaga gcctgaacgg gttcttcatc ctgtcccgca cgatcggatt catcgggcat
      781 tgggtggacc agaagcggca ggggagccgg ttgattcgtc tcttcgatta tctggtgaat
      841 tatgcgacgc cgaagcggcg cgaggtcccg ccgttggggt agcccgtcca tgagcggcca
      901 tctgcggcgt tgccgctgcg cttccgctcc tcacgtacgc attcagtacg ctccggcgcg
      961 gttctcgcgg cgccttgcat ctggcgcgct cctgaacggg ctccgctgtc gcggggactt
     1021 ctaaggcgtg gttggttggc agaaggggag ttgtttgctg tagtctagac gtgatggtcg
     1081 tgaggtagga cagagtgaac gagtcaaata ccatacagtc gccccaagaa tccggccgga
     1141 tgccgaagtt cgtcaccgtg gaggtgatgg gcaagaagca tcaggtgccc gagggcgtca
     1201 ccgtgatcca ggcgctctgg tacacgggcc acgagatgat ccgcggcatc gggtgtctgg
     1261 gcgggacgtg cggggcgtgc gggatggtgt accggatgcc gggccagtac gagctgctca
     1321 acggcttggc ctgccagacc acggtgcgcg acggcatgtg gttctccatg gtggcgaact
     1381 accccgcggc gcaggcgcac taccgcatcc cggagctgac caacgccaag gagcagctct
     1441 tcgagatgta tccggagtcc gcgcgctgcg tgaactgcaa cgcgtgcaac cgggtgtgcc
     1501 cgcagggcat cgacgtgcgc agcagcatct ggtacgcggt gttcggcgac tttcagaagg
     1561 tcgcggacct gaccatgagc tgcaacaatt gcggcctgtg catcacccgc tgcgtggcgg
     1621 acatgccgcc caatctggtg gggttgtacg cccgccggac ctacggcgcg ctctacgcgc
     1681 ccgcggccga ggaactcgcc cgccgcgtct ccgacatcgg ggacgggaag taccgcgacg
     1741 aactcgaccg gttgttgtcg gcggattccg ccgaactcaa atcgcgggcg gccgcggtcg
     1801 gccgctgact gccgtgagcc tcgatcccat cgccgaatcc cgcggccgcg cggacgcgac
     1861 gcgccaggcc cgcaccggcc ggaccgttcc gccgttgccg c
//
LOCUS       DBNGTT010000011         1814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1535433, whole genome shotgun sequence.
ACCESSION   DBNGTT010000011 DBNGTT010000000
VERSION     DBNGTT010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1535433"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..910)
                     /locus_tag="AC16BC_00200"
     CDS             complement(104..910)
                     /locus_tag="AC16BC_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3240058.1"
                     /translation="MTDQSLDQYRIAKEPFYVPVRDEVRLFELAARSRTPVMLKGPTG
                     CGKTRFVEHMAYRLQRPLITVACHEDLTASDLVGRYLLRGDETVWVDGPLSLAVRHGA
                     VCYLDEVVEARKDTTVVIHPLCDARRILPVEKRGEVVRAHDDFLLVISYNPGYQSLIK
                     ELKPSTKQRFLALEFDYPPADTERHIVQHEGGVGESVAASLVKLAAKVRHLKHHGLDE
                     GVSTRLLVYAAALIRDGIAPAEACQVAIAQPITDDRDMQRAIAELVATIF"
     gene            1050..>1814
                     /gene="purU"
                     /locus_tag="AC16BC_00205"
     CDS             1050..>1814
                     /gene="purU"
                     /locus_tag="AC16BC_00205"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HIR3240059.1"
                     /translation="MSRVRFLVSCPDRPGIVAAIARTICDLGGNILQSDQHTTDPSGG
                     EFFMRVEFTLPEPVARAALGAAVDRLTHDFRMQIAVQFAGEKKRVAIFVSRLEHCLVD
                     LLWRWKTGELPMTIAAVVSNHEDLRHAVAPFGLPFTVFPVTPDTKREQEDEILACLDG
                     QVDLIVLARYMQILTPTFVAAYPSRIINIHHSFLPAFVGGRPYEQAAKRGVKIIGATA
                     HYATAGLDEGPIIEQDVVRVSHRDGVADLMRKGKDIE"
BASE COUNT          323 a          562 c          599 g          330 t
ORIGIN      
        1 tgtgccgatg cctctgagcg tcagaatgct cacgctggac cgcacattgg gaaattgaca
       61 ggaaaaagaa ggaaggaaaa ggtcttttcc gccgaacaac tactcagaaa atcgtggcga
      121 ccagttcggc gatcgcgcgc tgcatgtccc gatcatcggt gatcggctgg gcgatcgcca
      181 cttgacaggc ttcggcaggc gcgatgccgt cgcgaatgag cgcggccgcg tacacgagca
      241 gtcgtgtgct gaccccttca tccaacccgt ggtgcttgag gtggcgcact ttcgcggcga
      301 gtttgaccag ggacgcggca acggactcgc ctacgccgcc ttcatgctgt acgatgtggc
      361 gctcggtgtc agccggtgga tagtcgaatt cgagcgccag aaagcgttgc ttggtgctcg
      421 gtttgagttc cttgatcagg ctctggtacc cggggttgta cgaaatcacg agcaggaagt
      481 cgtcgtgggc gcggaccacc tcgccgcgtt tttcgaccgg gagaatccgc cgggcgtcgc
      541 acaacgggtg gatcaccacc gtggtatcct tgcgcgcctc gaccacctcg tcgagataac
      601 agaccgcgcc gtgccggacc gcgagggaca gcggaccgtc gacccacacg gtttcgtccc
      661 cgcgcagcaa ataccggccg accaggtccg acgcggtgag gtcttcatgg cacgccaccg
      721 tgatcaaggg ccgttggagc cgatacgcca tgtgctcgac gaaccgggtc ttgccgcaac
      781 cggtcgggcc tttgagcatc accggggtgc gggagcgcgc cgccaactcg aacagacgga
      841 cttcgtcgcg caccggcacg tagaagggtt ccttggcgat gcgatactga tcgagcgatt
      901 ggtcggtcat gcgtgccctc gtgatttccg cgctgcattg taacgggtcg aaccggccgg
      961 cgcaacggcg gatatcgcgc gccgtggcgc gggagcgccc ggtcttctat aatgaacgcg
     1021 cggcgacgag catcccgcga ggaggtttcg tgtcccgagt gcgttttttg gtgtcgtgtc
     1081 ccgatcgtcc cggcattgtc gccgcgatcg cccgaacgat ctgcgatctg ggcggcaata
     1141 tcctgcaatc cgaccaacac accacggatc cctccggtgg tgaattcttc atgcgcgtgg
     1201 agttcaccct cccggaaccg gtggcgcgcg cggcgctggg cgccgcggtg gatcggctca
     1261 cgcacgactt tcgcatgcag atcgcggtgc aattcgccgg ggagaaaaag cgggtcgcca
     1321 ttttcgtgtc gcgcctggaa cactgcctcg tggatttgct ctggcggtgg aagacgggcg
     1381 aactccccat gacgatcgcc gcggtcgtga gcaaccacga ggatctgcgg cacgcggtgg
     1441 cgcccttcgg attgccgttc acggtgttcc cggtcacccc cgacaccaaa cgcgagcagg
     1501 aggacgagat cctcgcgtgc ctggacgggc aggtcgatct gatcgtgctc gcccggtaca
     1561 tgcagattct cacgccgacg ttcgtggccg cgtatccgtc ccgcatcatc aacatccacc
     1621 atagttttct gcccgcgttc gtgggcggcc gaccctacga gcaggcggcg aagcggggcg
     1681 tgaagatcat cggcgccacc gcgcactacg ccaccgcggg gctggacgaa ggcccgatca
     1741 tcgagcagga cgtggtgcgg gtgagtcacc gcgacggggt ggcggacttg atgcgcaagg
     1801 gcaaggacat cgag
//
LOCUS       DBNGTT010000012         4969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1535909, whole genome shotgun sequence.
ACCESSION   DBNGTT010000012 DBNGTT010000000
VERSION     DBNGTT010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1535909"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..593)
                     /locus_tag="AC16BC_00210"
     CDS             complement(45..593)
                     /locus_tag="AC16BC_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240060.1"
                     /translation="MRRLIGMGIGACTLAAWFGGAGLVLAESPVTPPAATTVPSVGIV
                     PADILIEVFLAPEHREAIPVIKKEFEALSVTRVRPQVFRLGNPPKNIAIGKDVPAPVA
                     RLALRLAVDYNRGVDFLLPEYRYFPKHIVIGSSAFDEASQIPIKPEDLQQLRDPSLTT
                     EQFHVLYRKLTGEDKRLPTYLK"
     gene            685..1158
                     /locus_tag="AC16BC_00215"
     CDS             685..1158
                     /locus_tag="AC16BC_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240061.1"
                     /translation="MATMIGKHGAALALLWATVWTAAGSGAFELARAAEAPLNVRIQD
                     LLDHSDQYDGRLVVVTGYVGHVAWERGRRGSEYVELVLEEAGSGVPPKVATVTVVALT
                     PPRVRACQHVLIQGVYHREGKQAGRPYEFFIDADAVIPDIPPESSRVPIDCEQKL"
     gene            complement(1181..2128)
                     /locus_tag="AC16BC_00220"
     CDS             complement(1181..2128)
                     /locus_tag="AC16BC_00220"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR3240062.1"
                     /translation="MTDYLDFERPLAELEEKIERLKGMAADQPKSQEEIQKLHKRAQQ
                     LRRDIYGRLTPLQTLQIARHANRPSTLDYLGHLIPDFVELHGDRLFADDSAIVGGVGR
                     LDGSPVVVIGHQKGRNVRERVKRNFGMPHPEGYRKAQRLMRMAERFKRPVITFIDTPG
                     AYPGVGAEERGQSEAIASSLRLMAELGTPIIAIVIGEGGSGGALALGVADRILMLEYS
                     VYSVISPEGCAAILWKSPDKTAEAADALKMTAKDLLALGVVDQIISEPPGCAHRDPAA
                     AAASIKQAIAKHLAELADRTAEELISARYDKFRKMGVVQ"
     gene            complement(2125..2583)
                     /locus_tag="AC16BC_00225"
     CDS             complement(2125..2583)
                     /locus_tag="AC16BC_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240063.1"
                     /translation="MFPDLFAASGALLGSDQPLLIDGTLDKGDKGIKIKATRIMSLES
                     ARERAGGHLEIMVDEGVGTENLRRLKATLERHRGTFPVWLRVLLRQHGRESTIVVGES
                     LRVNPTRALLDEIEAGFGKGSACVRSDPHRGLARPIRSTPRRRAGAYAAR"
     gene            complement(2486..>4969)
                     /gene="dnaE"
                     /locus_tag="AC16BC_00230"
     CDS             complement(2486..>4969)
                     /gene="dnaE"
                     /locus_tag="AC16BC_00230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3240064.1"
                     /translation="LERQDAKAHDVLLCLQTGKTLQDPNRMRFSTEELYVKSPDDMAR
                     LFSELPDAVKQTVAIAERCNLKLDLGTFHLPHYAVPDGTTREAHMEHLAVAGLRERLT
                     RLTPDAATAAMYEQRLRDELAMINAMGSAGYFLIVWDIINFARTRGIPVGPGRGSAAG
                     SLAAYALQITDIDPIRYGLIFERFLNPERVSLPDIDMDFCMDRRGEVIDYVTQKYGTD
                     HVAQIITFGTMAAKAAIRDVGRVLDVPYAEVDKVAKLVPTTLNITLDEAIAQEPKLKE
                     LSDTDPKINELLSLAKALEGITRHASTHAAGVVISKAPLTEHVPLYRGVNGETVTQFA
                     MTEIEKIGLVKFDFLGLRTLTVIDHALKILNERRPDQPPLMAADIALDDAKTYELLGT
                     GQTTGIFQLESSGMRDLLVKMRPERFEDLIAILALYRPGPIGSGMLDDFIKRKRGQTK
                     IQYELPELEDLLKETYGVIVYQEQVMKIANRLAGFSLGDADLLRRAMGKKLPEEMEKQ
                     KALFIAGATKNGFDPKKAEKIFDLMAYFAGYGFNKSHSAAYALITYQTAYLKAHHPLE
                     FMAALLTSEMGNEDKIVQYIGECRAMGIAILPPDVNESEQVFTIADQAIRFGLVAVKN
                     VGAGAIESVLNARAEHGRFRSLAHFCREVDLRKVNRRATRATDGRVGSDHGSRGRLTV
                     RPPGGTRNPVRRNGRRPVGRRRAAGRAGMGRLHAGAVREGEPRLLHHQPPARALRAGD
                     GAIRGDPDRPPGRRGRRRRRAGLGRAREQEDHHHQKGRPHGLRAGRRPRGQRRDHRVP
                     GSLRRVRRAPGVRSAPADRRDPGQGRQGH"
BASE COUNT          751 a         1576 c         1680 g          962 t
ORIGIN      
        1 tttggaaaag gggggatggg gggatttgtt tctcggagcc gaatctactt caggtacgtc
       61 ggcaggcgtt tgtcctcgcc cgtgagcttc cgatacagca cgtggaactg ttcggttgtg
      121 agcgacgggt cgcgaagctg ctgaaggtct tcgggtttga tggggatctg cgaggcttcg
      181 tcgaacgccg agctgccgat cacgatgtgt ttggggaagt accggtattc cggcaagaga
      241 aagtccacgc cgcggttgta gtccacggcg agccggagcg cgaggcgcgc gaccggagcc
      301 ggcacgtcct tcccgatcgc gatgtttttc ggaggattcc ccagacgaaa tacctgcggc
      361 cgtacccgcg tgaccgaaag cgcctcgaat tctttcttga tcaccggaat ggcctctcgg
      421 tgttcgggcg caaggaacac ctcgatcagg atatcggcgg gcacgatccc cacgctgggc
      481 accgtggtcg cggccggtgg agtcacaggc gactcggcga gcacgagtcc tgctcctccg
      541 aaccacgcgg caagggtgca cgcgccgatt cccatcccga taagacgtct catccgtcga
      601 gttcctttcg cggttgtgaa gggatgccgc attataccat cgcgcatgtg gcgcagtgga
      661 accgggagtg atagagtttc gtcgatggcg acgatgatcg gcaaacacgg cgcggcgctc
      721 gccctgctct gggcgaccgt ctggacggcc gccgggtcgg gcgcgttcga acttgctcgg
      781 gcggcggaag ctccgttgaa cgtcaggatt caggacctgt tggatcactc ggatcagtat
      841 gatggccgtc tcgtggtggt gacgggatac gtggggcacg tcgcgtggga gcgcggccgg
      901 cgcgggagcg agtacgtgga gctcgtactg gaggaagccg ggtccggcgt gccgcccaag
      961 gtggcgacgg tgaccgtggt cgcgctgacc cctccacgag tccgcgcctg ccagcacgtg
     1021 ctcatccaag gcgtgtacca ccgcgagggc aagcaggcgg gacgtcccta cgagttcttt
     1081 atcgacgccg acgccgtgat cccggatatt ccgcccgagt cgagtcgggt cccgatcgac
     1141 tgcgaacaaa aactctgata cccagggacg tcggccgcgg ctactgcacc acgcccattt
     1201 tgcggaactt gtcgtagcgc gcggagatca attcctcggc ggtgcggtcg gccaactcgg
     1261 cgaggtgttt ggcgatggct tgtttgatcg aggccgcggc ggccgcgggg tcgcggtggg
     1321 cgcagcccgg cggctcggag atgatctggt cgaccacgcc cagcgccagc aggtccttgg
     1381 ccgtcatctt cagggcgtcg gccgcttctg cggtcttgtc cgggctcttc cagagaatcg
     1441 cggcgcagcc ctcggggctg atcaccgagt agaccgagta ttccagcatc aggatgcgat
     1501 cggcgacgcc caacgcgagc gcgccgccgc tgccgccttc gccgatcacg atcgcgatga
     1561 tgggcgtgcc cagttccgcc atcagccgca ggctggacgc gatggcttcg gattggccgc
     1621 gttcttccgc gccgacccct gggtacgcgc cgggcgtgtc gatgaacgtg atgaccgggc
     1681 gtttgaagcg ttcggccatg cgcatgaggc gctgcgcctt gcgataccct tcggggtgcg
     1741 gcatcccgaa gtttcgcttg acccgttcgc gcacgttgcg gcccttctgg tggccgatca
     1801 ccaccaccgg cgagccgtcc aatcgtccca ccccgcccac gatcgcggaa tcatccgcga
     1861 acaagcggtc gccgtgcagc tccacgaaat cgggtatcag gtggcccagg taatccagcg
     1921 tgctgggacg gttggcgtgc cgggcgatct gcagcgtctg cagcggagtg aggcgcccgt
     1981 agatgtctcg gcggagttgc tgggcgcgct tgtgcagctt ctggatctcc tcctgggact
     2041 tgggctgatc ggcggccatg cccttgaggc gctcgatttt ttcttccagc tccgcgagcg
     2101 gccgttcgaa atccagatag tcggtcatcg ggcggcgtag gctcccgcac gccgcctggg
     2161 cgtgctgcga atcggtcgcg cgaggccgcg gtgcgggtcg ctgcgcacgc acgcggaccc
     2221 cttgccgaac ccggcctcga tctcgtcgag caaggcgcgg gtggggttca cgcgcaatga
     2281 ctcgccgacg acgatggtgg actcgcgtcc gtgttgccgc aacaggaccc gcagccagac
     2341 cgggaacgtg ccccggtgcc gctcgagcgt ggccttgagg cgccgcaggt tctccgttcc
     2401 caccccttca tcgaccatga tttccaggtg gcctccggcg cgttcgcgcg cggactccaa
     2461 cgacatgatc cgcgtggcct tgatcttaat gcccttgtcg cccttgtcca gggtcccgtc
     2521 gatcagcagg ggctgatcgg accccaggag cgcgccggac gcggcgaaga gatccgggaa
     2581 cacgatggtc tcgacgctgc ccgcgaggtc ttcgacccgc acgtaggcca tgcggtcgcc
     2641 ctttttggtg gtggtgatct tcttgctcgc gagcacgccc cagacccgca cgtcgtcgcc
     2701 gtcgcccgcg tcggccaggc ggtcggtcgg ggtcaccgcg aatcgctcca tctcccgcgc
     2761 gtagggcgcg agcgggtggc tggtgatgta aaagccgagg ctctccttct cgcaccgcgc
     2821 cagcgtggag tcgtcccatt ccggcgcgtc cggcagcgcg tcgtcggccg acgggccgtc
     2881 gcccgttccg ccgaacaggg tttcttgtcc cgcccggcgg gcggactgtg agccggccgc
     2941 ggcttccatg gtccgatcca acgcgtccat cagttgcgcg cgtcgcgcgc cggttgacct
     3001 tgcgcaggtc cacctcgcgg cagaagtgcg ccagagagcg gaagcggccg tgttcggcgc
     3061 gcgcgttgag cacggattcg atcgcgccgg cgccgacgtt tttgaccgcg accagtccga
     3121 atcggatcgc ctgatccgcg atcgtgaaca cctgttcgct ctcgttgacg tcgggcggca
     3181 ggatcgcgat gcccatggcg cggcactcgc cgatgtactg cacgatcttg tcctcgttgc
     3241 ccatttcgct ggtgagcaac gcggccatga attccaaggg atggtgcgcc ttgaggtacg
     3301 cggtctggta cgtgatcagc gcgtaggccg cgctgtggct cttgttgaag ccgtaccccg
     3361 cgaaataggc catcagatcg aagatcttct cggccttctt ggggtcgaag ccgttcttgg
     3421 tcgcgcccgc gatgaacagg gctttttgct tctccatttc ctcgggcagt ttcttgccca
     3481 tggcgcgtcg cagcagatcc gcgtcgccga gcgaaaaccc ggcgaggcgg ttggcgatct
     3541 tcatcacctg ctcctggtac acgatgaccc cgtacgtttc cttgagcagg tcctccaact
     3601 cgggcagttc gtactggatc ttggtctgcc cgcgtttgcg tttgatgaag tcgtccagca
     3661 tgccgctgcc gatgggaccc ggccggtaca acgccaggat cgcgatcaag tcctcgaatc
     3721 gctcgggccg catcttgacc agcaggtcgc gcatgccgct gctttccaac tggaagatcc
     3781 cggtggtctg cccggtgccc agcagctcgt aggtcttggc gtcgtcgagc gcaatgtccg
     3841 cggccatcag cggcggctgg tcgggccgtc gctcgttgag gatcttgagc gcgtggtcga
     3901 tcacggtgag cgtgcgcaga ccgagaaaat cgaacttcac caagccgatt ttttcgatct
     3961 cggtcatggc gaactgggtg actgtttcgc cgttgacccc gcggtacaag ggcacgtgtt
     4021 cggtcagggg cgccttggag atcaccaccc ccgcggcgtg cgtggacgcg tgccgggtga
     4081 tgccttcgag cgccttggcg agcgagagca attcgttgat cttggggtcg gtgtcggaca
     4141 gctccttgag tttcggctcc tgggcgatgg cctcgtccag cgtgatgttg agcgtggtgg
     4201 ggaccagctt ggcgaccttg tccacctcgg cgtagggcac gtccagcacg cggcccacgt
     4261 cgcggatcgc ggccttggcc gccatggtgc cgaacgtgat gatctgcgcc acgtggtcgg
     4321 tgccgtactt ctgcgtgacg tagtcgatca cctcgccgcg ccggtccatg cagaagtcca
     4381 tgtcgatatc gggcaggctg acgcgctcgg gattcaggaa ccgctcgaag atcagaccgt
     4441 agcggatggg atcgatgtcc gtgatctgca gcgcgtacgc ggccaggctt ccggccgcgg
     4501 acccgcgtcc cggacccacc ggaatgccgc gcgtgcgcgc gaagttgatg atgtcccaca
     4561 cgatgagaaa gtaccccgcg gaccccatcg cgttgatcat ggccaattcg tcgcgcaggc
     4621 gctgctcgta catggccgcg gtggcggcat ccggcgtcaa gcgggtgagg cgctcgcgaa
     4681 ggccggctac ggccaagtgc tccatgtgcg cctcgcgcgt ggtgccgtcc ggcaccgcgt
     4741 aatgggggag gtggaacgtg cccagatcca gtttcagatt gcagcgctcc gcgatcgcga
     4801 ccgtctgttt gaccgcgtcc ggcaattcgc tgaacaggcg cgccatgtcg tcgggcgatt
     4861 tgacgtagag ctcctcggtc gagaagcgca tccggttcgg gtcctgcagc gtcttgccgg
     4921 tttgcaggca caacaacacg tcgtgcgcct tggcgtcctg gcgctcgag
//
LOCUS       DBNGTT010000013         3726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1280465, whole genome shotgun sequence.
ACCESSION   DBNGTT010000013 DBNGTT010000000
VERSION     DBNGTT010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1280465"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC16BC_00235"
     CDS             complement(<1..653)
                     /locus_tag="AC16BC_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3240065.1"
                     /translation="MIPISTDVVIVGGGVAGLRAAIEAARTRRVVVINKERGNKSNSA
                     YAQGGIAVALGGREERASHVADTLRTGRGLCRPDAVRILVDEGEQRVRELIRWGVRFD
                     RDGRRYAKAHEGAHSRRRILRAGGDRIGVEMTSVLLRLVESHPRITTAAGVFSVDLCV
                     ENGRCVGVWVLDRGRFRLIRAGAVILATGGIGQAYWRTTNPAVATGDGLAMAYRAGAV
                     "
     gene            complement(665..853)
                     /locus_tag="AC16BC_00240"
     CDS             complement(665..853)
                     /locus_tag="AC16BC_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240066.1"
                     /translation="MDPETATVYLRWDGEWRAAGRAPVVRQGAARFVVETGEHTALAA
                     VLGDTPFGPVSAGSPIPR"
     gene            complement(860..1126)
                     /locus_tag="AC16BC_00245"
     CDS             complement(860..1126)
                     /locus_tag="AC16BC_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240067.1"
                     /translation="MSWRRRVAVVLLVALSACAGAPKLTDDGRRVQRVDAFLGELDHA
                     YERRDATAVMAALSPRFADRDALGRQIDSTFAEFNDIELAFAIE"
     gene            1250..1339
                     /locus_tag="AC16BC_00250"
     CDS             1250..1339
                     /locus_tag="AC16BC_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HIR3240068.1"
                     /translation="MSRVVKKRRKKMRKHKYKKLRKKMRHKRK"
     gene            1409..2752
                     /locus_tag="AC16BC_00255"
     CDS             1409..2752
                     /locus_tag="AC16BC_00255"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3240069.1"
                     /translation="MLTRGFAAIVITSMAMSGCATSRDQAPPAESSAPPPSKAYGRAF
                     LQEARQTYRFILDPDITDAVHATGTRLVRAMGEDPEAIHFFVIDEPQPNAFAIPGGYI
                     FVFTGLLSKLSDDEELAGVLAHELGHVAYNHFFQNRNQILALNLATVAALLLSGGHPA
                     ALAFGTGASFHLQLAYSREHEAEADAAAVRFLRKARYDPSGLARFFRTLEVYERFNPP
                     LVPAYFTTHPGVSDRLRVVETTIEGTAPPARDPDAPIIDWGRLRAGLGVAPDERATSP
                     RDHYLRGVNHLKSDRIEAAIEEFHAVLAVEPHHGAARADLAWALLRAQRRDDARAEAR
                     LAQRDAPDLAGPDIVLGVLAQDAGDHQAAIQYLLDAVTRSPDDPTTHLRLASSYAALG
                     DSPHEIYHLGRHFRLSLLPDRATLLFRQVLSNPQTDPELKRVAEHELAIIAREGV"
     gene            2828..3370
                     /locus_tag="AC16BC_00260"
     CDS             2828..3370
                     /locus_tag="AC16BC_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR3240070.1"
                     /translation="MIASPIRLSDIPTEGVSLVFRCDRSLLEPLGEGVHLTEPVDVSL
                     RITPQDDRYFIEGEVRGTVQIECDRCAAPVKLDVRAPCAIDAVPLPTGGTSGERSELD
                     RGELDVMFVPGSLLNVEDLVREQLLLQVPMRVLCREGCAGLCPRCRKNLNEGPCGCPE
                     TAAIDPRLEILKKVRRLPER"
     gene            3432..3614
                     /gene="rpmF"
                     /locus_tag="AC16BC_00265"
     CDS             3432..3614
                     /gene="rpmF"
                     /locus_tag="AC16BC_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR3240071.1"
                     /translation="MANPKHKISRARRDKRRAHHALGNPTLSACPQCREPKLPHRVCP
                     SCGTYRGRDVIPVKEV"
BASE COUNT          636 a         1315 c         1186 g          589 t
ORIGIN      
        1 acggcgcccg cgcgataggc catggccagc ccgtcgccgg tcgcgaccgc ggggttggtc
       61 gtgcgccaat aggcctgacc gattccgccg gtcgcgagga tcacggctcc ggcgcgaatc
      121 agacggaagc gtccgcggtc caagacccac acccccacgc atcggccgtt ctcgacgcac
      181 agatccacgg aaaacactcc ggccgcggtc gtgatccggg gatgtgactc aacgagtcgg
      241 agcaggacgc tggtcatctc caccccgatc cgatcgccac cggcgcggag gatccggcgg
      301 cgcgagtgcg cgccttcgtg cgccttggcg taccgccgac cgtcgcggtc gaagcgcacg
      361 ccccagcgaa tgagttcccg cacccgctgt tcgccctcat caaccaagat ccgcacggcg
      421 tccggccgac ataaacctcg cccggtacgc aacgtatccg cgacgtgcga ggcgcgttcc
      481 tcacgtccgc cgagcgccac cgcgatgccg ccctgcgcgt acgcgctgtt gctcttgttg
      541 ccgcgctcct tgttgatgac cacgacgcgc cgggtcctcg cggcttcgat ggccgcccga
      601 aggccggcca cgccgccgcc cacgatcacc acatccgttg aaatgggaat cacgggaagg
      661 aaacttagcg ggggatgggg gagccggccg agaccggccc gaacggcgtg tccccgagca
      721 cggcagcgag cgccgtgtgc tcgccggttt ccaccacgaa ccgggccgcc ccctgtcgga
      781 cgaccggcgc gcgcccggcg gcgcgccact cgccgtccca ccgcaggtac accgtggccg
      841 tctccgggtc caactggatt cactcgatcg cgaacgccag ctcgatgtcg ttgaattccg
      901 caaacgtcga gtcgatctgg cggcccaacg cgtcacggtc ggcgaagcga ggcgagagcg
      961 cggccatcac cgcggtcgcg tcccggcgct cgtaggcgtg atcgagctcc ccgagaaatg
     1021 cgtccacgcg ttgaacccga cggccgtcat cggtcaactt gggcgcgccg gcgcacgcgc
     1081 tcagcgccac cagcagaacg accgcaaccc gacgccgcca ggacatgcgg cgcattatag
     1141 aaaccccgct ccagtgggtc aagccgcgcg cgccggagcg ccgcttgctt tccgcaaggc
     1201 actcgcccta caatgcgcgc cacggtatag cgaagatcgg ggaacgggca tgtcacgagt
     1261 tgtcaaaaaa cgccgcaaaa agatgcgcaa acacaagtac aagaagctgc gcaaaaagat
     1321 gcggcacaag cgcaagtaat ccggcctagc gcgggccacg gctttttggt acgctgaccg
     1381 ggtgcctggg aacaccccgt ggccccactt gttgacgcgg ggcttcgcgg cgatcgtgat
     1441 cacctcgatg gcgatgtcgg ggtgcgccac ttcacgagat caagcgcctc cggctgaatc
     1501 gagtgcgccg cccccatcca aagcgtacgg tcgcgccttt ctccaagaag cgcgtcagac
     1561 ctatcggttt atcctggacc cggacatcac cgacgccgtc cacgcgaccg gaacccgatt
     1621 ggtccgcgcc atgggcgagg accccgaggc gattcacttc ttcgtcatcg acgaaccgca
     1681 gcccaacgcg ttcgccatcc cgggcggcta catcttcgtc ttcaccggcc tgctctcgaa
     1741 attatccgac gacgaagagc tggccggggt cttggcccac gagttgggcc acgtcgccta
     1801 caaccacttt ttccagaacc gcaatcagat cctcgcgctc aacctggcca cggtggcggc
     1861 gctgttattg agcggaggcc atccggccgc gttggccttc ggcaccggcg ccagctttca
     1921 cctgcagctg gcctacagcc gcgaacacga agccgaggcc gacgccgcgg cggtgcggtt
     1981 tctgcgcaag gcgcggtacg acccttcggg gctcgcccgc ttctttcgca ccctggaggt
     2041 gtacgaacgg ttcaaccccc cgttggttcc cgcgtacttc accacgcacc ccggcgtgtc
     2101 cgaccggttg cgggtcgtcg agaccaccat cgagggcacg gcgccgcccg cgcgcgaccc
     2161 cgacgcgccg atcatcgatt ggggacgact cagagccggg ttgggcgtgg ccccggacga
     2221 acgcgccacg tccccccgcg accactacct gcgcggggtc aatcatttga agagcgaccg
     2281 gatcgaggcc gcaatcgagg aatttcacgc ggtcctcgcg gtggaaccgc accatggcgc
     2341 cgctcgcgcg gatctggcgt gggccctcct gcgagcccag cggcgtgacg acgcgcgggc
     2401 cgaggcccgg ctcgcacaac gggacgcgcc cgatctcgcg ggccccgaca tcgtgctcgg
     2461 cgtcctggcg caagacgccg gcgaccatca agcggccatt caatatctgc tggacgcggt
     2521 gacgcgttcc ccggacgacc cgaccacgca ccttcgtctg gccagctcct acgcggccct
     2581 gggggactcg cctcatgaaa tttatcacct cggcagacac ttccgactct cgttgctgcc
     2641 cgaccgggcg accctgttgt tccgccaggt cctctccaac ccccagaccg acccggagtt
     2701 gaagcgcgtc gcggagcacg agttggccat catcgcgcgg gaaggcgtct gacgcgctgg
     2761 gcgccgaccg cgcgtcgctt gacaagcccg gcggggattt cttatagtca gcccgctgtt
     2821 cacggctatg attgcatcgc cgatccggct ctctgacatc ccgaccgagg gtgtgtcgct
     2881 cgttttccgt tgcgaccgct ccctgctcga accgctgggg gagggggtgc acctgaccga
     2941 gccggtggat gtgtcgcttc ggatcacgcc gcaggacgat cgatatttca tcgagggcga
     3001 ggttcgcggg acggtgcaga tcgagtgcga ccgttgcgcc gcccccgtga agttggatgt
     3061 gcgcgctccc tgcgcgatcg acgcggtgcc gttgcccacg ggcgggacgt cgggcgaacg
     3121 cagcgagttg gaccggggcg agttggacgt gatgtttgtc cccggttcgc tgctcaacgt
     3181 ggaagatctg gtccgggagc agttgttgct gcaggtcccg atgcgggtgt tgtgccggga
     3241 agggtgcgcg gggctgtgtc cgcgctgccg gaaaaatttg aatgagggtc cgtgcgggtg
     3301 tccggaaaca gcggccatcg acccacggtt ggaaatactg aagaaggtcc ggcgtcttcc
     3361 ggagcgttga ccacggacca gcggttcgat cacctgaact gtttgtagac ggtcgagaga
     3421 aggacgtact catggcaaat cctaaacaca agatctcccg cgcgcgacgc gacaaacgac
     3481 gcgcgcacca cgcgctgggc aaccccacgc tctcggcctg cccgcagtgc cgggagccca
     3541 agctgcccca tcgagtctgt ccgtcctgcg gcacctaccg cgggcgcgac gtgattccgg
     3601 tcaaagaggt ctaacccttc ccccaccgcg tggcttccca cacgtcgcca ggcggacgca
     3661 ccgaccccgg ccggccgacc agcgatcggt cggcggattc gattccaacg gccacgatcg
     3721 ccgtcg
//
LOCUS       DBNGTT010000014         3872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2685799, whole genome shotgun sequence.
ACCESSION   DBNGTT010000014 DBNGTT010000000
VERSION     DBNGTT010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2685799"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..963
                     /locus_tag="AC16BC_00270"
     CDS             262..963
                     /locus_tag="AC16BC_00270"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3240072.1"
                     /translation="MLAPVAVASASPSSFPMASAAPPRRAATEPPQLGERRITVNKDA
                     QLPAVIMGYHVPNYRHQDSYALTVLAALLTQGESSRLHRALVYDREVALEVGGDYTAL
                     SADPPIFYLYGMTHAGKSAADLERAVIEEVDRIKTAEPTERELTKVKNGIEAEFILGQ
                     DSNFYRAMQVGTVETVGAGRGYLDGFVANIRKVTAADVVRVANAYLNQNNRTVGVLNP
                     LRAQEPPAAPDAVAP"
     gene            960..2336
                     /locus_tag="AC16BC_00275"
     CDS             960..2336
                     /locus_tag="AC16BC_00275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3240073.1"
                     /translation="MSTRSVARWRPVAAAAILWSAWLPAAIAAPVSVDRWVMGNGLTV
                     LFVERHAIPAVQAHLVIKTGATADPAGRTGLAALTAGLITKGTPTRSAVDIAEAMDFI
                     GGSLSSSAAEDFSAVTMTALKKDLPAALTLMSDVVLRPSFPAAEVERVRRETLSGILA
                     GKDNPEAVAEKAFAPLVFGAHPYARPVEGLESTVPTLTREEAVAFHRAYYRPNNAVLV
                     LVGDLTPRDARRAAKTAFGEWERNAIPPMSVPSIPALRDRRVELIDKDLTQATVVLGH
                     VGIARMDPDFYAATVMNYILGGGGFASRLMTRIRDNEGLVYGISSGFDAKRFPGSFSV
                     GLQTKTESAPAAIRAVLEEIAKVRSEGVTAAELEGAKSYLAGSFPLRYETNSRLAGLL
                     GMVELYGLGLTYFEDYPKKIRAVTLEDVRRVATRRLDTERYVLVVVGRTHEIAAALNH
                     PPAPGDAR"
     gene            2333..3139
                     /gene="larE"
                     /locus_tag="AC16BC_00280"
     CDS             2333..3139
                     /gene="larE"
                     /locus_tag="AC16BC_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR3240074.1"
                     /translation="MNASTPKLDRLYAVLRDLDSVLVAFSGGVDSTLLAKAAHDALGP
                     RAVAATALSPTYPARQVEQAAALAQHIGIRHEWVRSEQMERPRFVANPADRCYHCKDE
                     LFDRLAPVAESLGLRHVAEGTNVDDLADHRPGLVAARERTIKSPLVDAGLTKSEIRAI
                     SRELDLPTWDQPASPCLSSRFPTGLPITIERLGQVDRAEEVLREHGFREFRVRHHDQT
                     ARIEVPAAEFARFADERVREAVVSSLKVLGFRFVTVDLSPFRSGSLHGAS"
     gene            3174..3773
                     /locus_tag="AC16BC_00285"
     CDS             3174..3773
                     /locus_tag="AC16BC_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240075.1"
                     /translation="MATATIGQAQESPGTVPALAADLEQRVLSSDGSVVSVTTSSVLL
                     TDAAARVESAGEPERAGYGEYHLYDFSRGRSYRIFPNDHIYFEAAWSSASAAKAFIEG
                     WAPKPETWSVRMIPLKDDVLDGTPTHLSLLERRLKSGPAPAYAFVWNVVPAGRLPARV
                     IYSQAGGQTVIVSYRRVESVRVDPSRFVVPAGFGNLSPF"
BASE COUNT          577 a         1268 c         1365 g          662 t
ORIGIN      
        1 cgaggcgaag ccgtgctttg tctttgaggt gaccatccgt ctattaactg cctcactccg
       61 tctccatatc tcgactcgct ccggccggtc gccgcgcccg gtcgccgcca gcgtcggcgt
      121 cggcgccggg ccgcgtggcg tccgccggac gtcgcggaca tcggcgcaca tcggcccgcg
      181 cgttgccgac gccggggccg acgacaacgc cacatccggc gctggccggg ccgtgtcggc
      241 cgggagtggt cacccggcgt cgtgctcgcg cctgtcgccg tcgcgtcggc gtcgccgtcg
      301 tcattcccca tggcgtccgc cgcgccgccg cgccgcgcgg ccacggagcc accgcagctc
      361 ggggagcgcc ggatcacggt gaacaaggac gcccaactgc cggcggtcat catggggtat
      421 cacgtgccga actatcgcca ccaggacagc tatgccttga cggtgctggc cgcgttgctc
      481 acccagggcg agagttcgcg gctgcaccgc gctctggtgt acgaccggga agtggcgttg
      541 gaggtcggcg gggactatac ggccttgtcg gccgacccgc cgattttcta cctgtacggc
      601 atgacccacg cgggcaagtc cgccgcggat ctggagcgcg cggtgatcga ggaagtggac
      661 cggatcaaaa ccgcggagcc gaccgaacgc gaattgacca aggtcaaaaa cgggatcgag
      721 gcggagttta tcttgggaca ggactcgaac ttctaccgcg cgatgcaggt cgggaccgtg
      781 gaaaccgtgg gcgccggccg cgggtacctc gacggcttcg tggccaacat tcggaaggtg
      841 accgcggccg acgtggtgcg ggtcgccaac gcctacttga accagaacaa ccgaaccgtg
      901 ggcgtattga acccgttgcg ggcgcaggaa ccgcctgcgg ctccggacgc cgtcgccccg
      961 tgagcacccg gtccgtggcg cgatggagac ccgtcgcggc ggcggcgatc ctgtggagcg
     1021 cgtggcttcc ggccgccatc gccgctccgg tttcggtgga tcggtgggtg atgggcaacg
     1081 ggctgaccgt gctgttcgtg gagcggcatg cgattcccgc ggtccaggcc cacctggtga
     1141 tcaagacggg cgccaccgct gatcccgcgg ggagaaccgg cttggccgcg ctcaccgcgg
     1201 gtttgatcac caagggaacg cccacgcgtt ccgcggtcga catcgccgaa gccatggatt
     1261 ttatcggcgg cagcctgtcg tccagcgcgg ccgaggattt ttccgccgtg acgatgaccg
     1321 cgctgaaaaa ggatctgccc gccgccttga ccctgatgag cgacgtggtc ctccgcccgt
     1381 cgtttcccgc ggccgaggtg gagcgggtgc gtcgcgaaac cttgtccggt attttggccg
     1441 gcaaggacaa tccggaggcc gtggccgaga aagcgttcgc gccgctggtg ttcggagccc
     1501 acccgtatgc ccggccggtt gaaggcctgg aaagcacggt gccgacgctg acgcgggagg
     1561 aggccgtcgc ctttcaccgc gcgtactacc gtcccaacaa cgccgtcctg gtgctggtgg
     1621 gcgatctcac gccgcgtgac gcgcggcgcg cggcaaagac cgcgttcggc gagtgggaac
     1681 gcaacgccat cccgccgatg tcggtgccgt cgatccccgc gcttcgcgac cgccgcgtgg
     1741 agttgatcga caaggacctg acccaggcca cggtcgtact cgggcacgtc gggatcgcgc
     1801 gcatggaccc ggacttttac gcggccacgg tcatgaacta tatcctgggc ggcggagggt
     1861 tcgcctcgcg cctgatgacg cgcatccgcg acaacgaggg cctcgtgtac ggcatctcga
     1921 gcgggttcga cgccaagcgg tttcccggct cattttcggt cgggctgcag accaagaccg
     1981 aatccgcgcc cgccgccatt cgcgcggtgc tggaggaaat cgcgaaggtc cggtccgagg
     2041 gcgtcaccgc cgcggaactg gagggcgcca agagttatct ggccgggagc tttcccctgc
     2101 ggtacgagac caattccagg ctcgcggggc tgttggggat ggtggagctg tacggcctgg
     2161 gcctgacgta cttcgaggac tacccgaaga agatccgggc cgtgacgctg gaggatgtcc
     2221 gccgcgtggc gacccggcgg ctggataccg agcggtacgt cctggtggtg gtgggccgga
     2281 cgcacgagat cgccgccgcg ttgaatcatc cgccggcgcc gggggacgcg cgatgaacgc
     2341 gtccactccg aagctggatc gcctgtacgc cgtgttgcgg gacctggact cggtgttggt
     2401 ggcgttttcc ggcggggtgg acagcacgct cctcgccaag gccgcgcacg acgccctggg
     2461 cccgcgcgcg gtggccgcga ccgcgttgtc gcccacgtac ccggcgcgcc aggttgagca
     2521 ggccgctgca ttggcgcaac acatcggcat tcgccacgag tgggtccggt ccgagcagat
     2581 ggaacggccg cggttcgtcg ccaaccccgc ggatcgctgt tatcactgca aagatgaact
     2641 gttcgatcgc ctggcgccgg tggccgagtc gttggggctt cggcacgtgg ccgaaggcac
     2701 caacgtcgat gacctggccg atcaccggcc gggcctcgtg gcggcccgcg agcgcaccat
     2761 caagagccca ctggtggacg cggggctgac gaaatccgag attcgcgcga tcagccgaga
     2821 attggatctg ccgacctggg accagccggc gtcgccgtgt ttgtcctcgc ggtttccgac
     2881 cggactcccg atcacgattg aacggctcgg gcaggtggat cgcgccgagg aggtcttgcg
     2941 tgaacacggg ttccgggagt tccgggtgcg ccatcacgac cagaccgcgc ggatcgaagt
     3001 gccggccgcg gagtttgccc gctttgcgga cgaacgcgtg cgcgaggccg tggtatcttc
     3061 gctcaaagtc ctgggttttc gttttgtcac ggtggatctg tcgccgtttc ggtccggcag
     3121 tctgcatggc gcatcgtagg gcgcggtggc ggagcctttg gatcgcatgg ctcatggcga
     3181 cggcgacgat cgggcaggcg caggaatccc cgggaaccgt gccggcgttg gccgcggatc
     3241 tggaacaacg ggtgctgtcg tccgacggct ccgtggtgtc ggtgaccacg tcgagcgtcc
     3301 tgctgaccga cgcggcggct cgcgtcgagt cggcggggga gccggagcgg gcggggtacg
     3361 gcgagtatca tctgtatgat ttttcccgcg ggcgctccta tcggatattc ccgaacgacc
     3421 acatatattt tgaggccgcg tggtcgtcgg cgtcggccgc caaggcgttc atcgagggct
     3481 gggcgccgaa gccggagacc tggtcggtgc gcatgattcc gctcaaggac gacgtgctgg
     3541 acggcacgcc gacgcacctg tcgctgttgg agcgccgact caagtccggc ccggctcccg
     3601 cgtacgcgtt cgtgtggaac gtcgtgcccg ccggccgact gcccgcgcgc gtcatctaca
     3661 gtcaggcggg cggccagacc gtgatcgtgt cgtaccgccg cgtcgaatcc gttcgcgtgg
     3721 acccgtcgcg gttcgtcgtg cccgccgggt tcggcaatct gagtccgttt tgaccgccgg
     3781 cgttgtccaa tgatcccgtt tgatcgtctc ctgtccgttc cggcccatat ccaccacgcc
     3841 gcttatctaa tgaaggaccc gccgtccgag ag
//
LOCUS       DBNGTT010000015         2371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2558088, whole genome shotgun sequence.
ACCESSION   DBNGTT010000015 DBNGTT010000000
VERSION     DBNGTT010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2558088"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="AC16BC_00290"
     CDS             <1..467
                     /locus_tag="AC16BC_00290"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3240076.1"
                     /translation="VGEAPGAEEDAQGIPFVGAAGQLLTKIIEAMGLARDAVYIANII
                     KCRPPGNRNPRPEEIASCEPFLVAQLDIIRPKVICALGTFSAQTLLKTKDPISRLRGR
                     WHAYQGIPLMPTFHPAYLLRNPAEKKTVWADVQLVMTRLGLPTPPPGRGQGA"
     gene            469..957
                     /locus_tag="AC16BC_00295"
     CDS             469..957
                     /locus_tag="AC16BC_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR3240077.1"
                     /translation="MRQLWAPWRMTYIESDKSGPCILCQKPRASADRDNYILERTEHS
                     FVMMNLYPYSNAHLMVSPYAHVDRLAGLPQAAALDLTLTLARAVERLRAALKAEGFNV
                     GLNLGKPAGAGIDDHLHFHIVPRWNGDTNYMTLFAEVRVIPEHLDATYDRLLPYFREP
                     QP"
     gene            954..1253
                     /locus_tag="AC16BC_00300"
     CDS             954..1253
                     /locus_tag="AC16BC_00300"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HIR3240078.1"
                     /translation="MIRGVLAFLIAGVVLLVANENRHQLMMLELPFGFRTDPLAVASV
                     ILASVLAGALLVAIALVPAWLRASLRARRLRRELDTGESSRPTRGEPKSAADSAP"
     gene            1260..>2371
                     /locus_tag="AC16BC_00305"
     CDS             1260..>2371
                     /locus_tag="AC16BC_00305"
                     /inference="COORDINATES: protein motif:HMM:NF017039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS N-terminal domain-containing protein"
                     /protein_id="HIR3240079.1"
                     /translation="MGDQTPLMRQYEALKADHPGALLLFRVGDFYELFGDDAVVAAPI
                     LQIALTSRDKNKETGMPLCGVPYHAASGYIAKLLAAGKCVALAEQMEEPKPGTLVARD
                     VVRVLTPGTVLDDTLLPGSEHAYLAAVICDGDDAALARVDVSTGDFDVISARGPQAGA
                     LAADELARTDAREVLVGPGWAWPRRAGSVYQTCQADAFDPLEADRALREQFPSAAAWT
                     VLSPIERRAAGALLRYVRHTLKGRCAALAPPTRTAAGDALDIDEAAQRTLELVRGSDG
                     SRHTSLLAVLDETLTPMGSRLLRERLLHPLRARAAVESRLDDVAAFLADADARASTRE
                     ALRRIGDVERAVSRAAVGSSTPRDLALVGRSLEAAAG"
BASE COUNT          364 a          844 c          795 g          368 t
ORIGIN      
        1 tcgtcgggga agccccgggc gccgaggaag acgcgcaggg catcccgttc gtgggagccg
       61 cgggccagtt gttgaccaag attatcgagg ccatgggcct ggcgcgggac gccgtgtaca
      121 tcgccaacat catcaaatgc cgaccgcccg gcaaccgcaa cccgcgtccc gaggaaatcg
      181 cgtcgtgtga accgttcttg gtcgcgcaac tcgacatcat ccgccccaag gtgatctgcg
      241 cgctcggcac cttctcggcg caaaccctgc tcaaaaccaa agatccgatc tcgcgattgc
      301 gcggccgctg gcacgcctat caaggcattc cattgatgcc gacgttccat cccgcgtacc
      361 tcctgcgcaa cccggctgaa aaaaagacgg tgtgggccga cgtccaattg gtgatgacgc
      421 ggctcggcct tccgacgcct ccgccgggcc gcggacaagg agcctgacgt gcgccagctc
      481 tgggctccct ggcggatgac gtacatcgag agcgacaagt ctgggccgtg catcctgtgc
      541 cagaagcccc gggcgtccgc ggaccgtgac aattacatcc tggagcggac cgagcacagc
      601 ttcgtgatga tgaacctcta tccgtattcg aacgcccacc tgatggtgtc gccgtacgcg
      661 cacgtggacc gcttggccgg gctcccgcag gccgccgcgc tcgacctcac cctgacgctc
      721 gcgcgcgcgg tcgaacgatt gcgcgccgcg ctgaaagccg agggcttcaa cgtgggcctg
      781 aacctcggca aaccggccgg ggccgggatc gacgaccacc tgcactttca catcgtgccg
      841 cggtggaacg gcgacaccaa ctacatgacc ctgttcgcgg aggtccgcgt gatccccgaa
      901 cacttggacg cgacctacga tcggttgctc ccgtactttc gggagccgca gccgtgatcc
      961 gcggcgtgct cgcgttcctc atcgccgggg tggtgctgct cgtggcgaac gagaaccggc
     1021 atcaactcat gatgttggaa ctgcccttcg ggtttcgcac cgatccgctc gcggtcgcgt
     1081 cggtgatcct tgcgagcgtg cttgcgggcg cgctcctggt ggccatcgcg ctggtccccg
     1141 cctggctccg cgcctcgctg cgcgcaaggc gcctccgccg cgaactcgac acgggcgagt
     1201 cttcgcgacc gacccgcggc gagccgaagt cggccgcgga cagcgcaccc taaggagcca
     1261 tgggcgatca gaccccgttg atgcggcagt acgaggcgct caaggccgac caccccgggg
     1321 ccttgctgtt gttccgggtc ggcgacttct acgagctgtt cggcgacgac gcggtcgtgg
     1381 ccgccccgat cctccagatc gcgctgacct cgcgcgacaa gaacaaggaa accgggatgc
     1441 cgttgtgcgg ggtgccgtat cacgcggcgt ccgggtacat cgccaaactg ctcgcggccg
     1501 gcaagtgcgt ggcgttggcc gagcagatgg aggaacccaa gcccggcacc ttggtcgccc
     1561 gcgacgtggt gcgggtgctc accccgggca ccgtgctcga cgacaccctg ctccccggtt
     1621 ccgaacacgc gtatctcgcc gcggtcatct gcgacgggga cgacgcggcg ctggcgcggg
     1681 tggacgtgtc caccggcgac ttcgacgtga tctcggcgcg aggaccccag gcgggcgcgc
     1741 tcgcggccga cgaactcgcg cgaacggacg cgcgcgaagt gctggtcggc ccgggctggg
     1801 cgtggccccg tcgggcaggg tccgtctacc agacgtgcca ggcggacgcc ttcgatccgc
     1861 tggaggctga tcgcgcgttg cgcgagcaat tcccctcggc ggccgcgtgg accgtgctgt
     1921 ccccgatcga gcggcgagcc gccggcgccc tgctgcggta cgtccgtcac acgctcaagg
     1981 ggcgatgcgc cgcgctcgct ccgcccacgc gaaccgcggc gggcgacgcg ctcgacatcg
     2041 acgaagccgc acaacgcacc ctggaattgg ttcgcggcag cgacggatcg cggcacacct
     2101 cattgttggc cgtgctcgac gagaccctga cccccatggg cagccggctg ctgcgcgaac
     2161 gactcttgca cccgctccgc gcccgagccg ccgttgaatc ccgattggac gacgttgccg
     2221 cgttcctcgc ggatgcggac gcgcgcgcgt cgacgcgcga ggctctgcga cgcatcggcg
     2281 acgtggagcg cgcggtgtcg cgcgccgcgg tgggatcgtc caccccgcgc gacctcgcgt
     2341 tggtgggccg ctcgctcgaa gcggccgccg g
//
LOCUS       DBNGTT010000016         8355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1536352, whole genome shotgun sequence.
ACCESSION   DBNGTT010000016 DBNGTT010000000
VERSION     DBNGTT010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1536352"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1966)
                     /gene="ilvD"
                     /locus_tag="AC16BC_00310"
     CDS             complement(281..1966)
                     /gene="ilvD"
                     /locus_tag="AC16BC_00310"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3240080.1"
                     /translation="MSVDPKSRSRAITNGRDRAPARAMLKGIGFTDEDLARPIVGIAN
                     TWTETMPCNYGLRDLAVHVKRGIREAGGTPMEFNTVSVSDGVTMGTEGMKASLISREA
                     IADSIELMGRGYLFDAMIVLVGCDKTIPAGAMALIRLDTPGIVLYGGSIQPGHFHGKD
                     VTIQDVFEGVGANAAGRMSDADLKLLEDSACPGAGACGGQFTANTMAAVMEFIGLSVM
                     NTASVPATDPRKEKVGIRVGQTVMDMLQRDLRPSKILTRKAFENAIASVAATGGSTNA
                     VLHLLAMAREAGIPLTIDDFDVVSTRTPLIADLKPGGKYVAADLDRAGGIPLVAQRLV
                     KAGFLDGTQLTPTGRTIAEESAGAVETPGQVVLTPVDKPLKPTGGLVILKGSLAPEGC
                     VVKVAGHERLLHRGPARVFDREEDAMAAVTKRQIRENDVVVIRYEGPKGGPGMREMLG
                     VTAAIVGEGLGNSVALLTDGRFSGATKGLMAGHVAPEAAAGGPIAAVREGDMITFDIT
                     KRRLDVEVSDDELKNRLKSWKAPEPRYKTGVFAKYAGMVGSASEGAVTRVPVR"
     gene            complement(2137..2379)
                     /locus_tag="AC16BC_00315"
     CDS             complement(2137..2379)
                     /locus_tag="AC16BC_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240081.1"
                     /translation="MKTVKIRGDGSIKLPADVLRTFPPGSNVAVSWEDDTITLQRLAA
                     RKTSRVKKPVGGRQMSPEEISEEIHEIRREKRGRLT"
     gene            complement(2465..3271)
                     /locus_tag="AC16BC_00320"
     CDS             complement(2465..3271)
                     /locus_tag="AC16BC_00320"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR3240082.1"
                     /translation="MTRALGMVALMMIAVLVAPAIGRGEEPSVDDIVRLVRAQDDAID
                     DETADVEIRSTGAQGDARSTTLRLYWKNQRGRDGLIGLTLLVTQAPLNRKGESFLLRQ
                     AERAGDSQAWLYLPDLRQALRITIAGQDTQRPKRNSDLLLGFEQLGARLLGKAARSVA
                     GHEPLDGVDHLVIEERLDGDNDALRRFWVSPVNWTIAKIEYRGAGGRVDVTQSIAWQR
                     VGRAWVWKRVEVRAADPPGQTVVELRNVTVNTGLNDRVFTVNTMKSGRIP"
     gene            complement(3345..3767)
                     /locus_tag="AC16BC_00325"
     CDS             complement(3345..3767)
                     /locus_tag="AC16BC_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240083.1"
                     /translation="MNFDPAIVAWQVFCRVRASNELGEDLDAAIDAQERFSGGAGDEA
                     SDAYRELVAIGARHPEASAFGEFLVYTTWSHLMDETVAEHFRRGLALCRALLQRESDH
                     DAERLARLRAMEQSFRAGLGEKVDDPLDYDADTLKGGD"
     gene            complement(3794..4933)
                     /locus_tag="AC16BC_00330"
     CDS             complement(3794..4933)
                     /locus_tag="AC16BC_00330"
                     /inference="COORDINATES: protein motif:HMM:NF051835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR3240084.1"
                     /translation="MIRVLGGVAVVVLMAGSAGLAAEPDPSATRGPDVIASGGSHTCR
                     IEPDSTVACWGDNGFWQLGDGGDGRSASPVAVAGVAGATGLAAGDWHTCAIVKAGAVV
                     CWGKNDLMQLGTAKPRRSSRPVSVAVGPAVELAAAHSHTCARLADGSVWCWGGNKDDQ
                     LGVQAPPRQTSSAPVKVPGIERAVSIAAGNEHSCAAVADGRLFCWGDNHYGQLGHGIQ
                     RHNSFPPQPVAIQSEVAQVVAGTRHTCARLRDLTVQCWGDNVEGQVGNGTTTRALTPA
                     PVATVDANGTAVPLADVERLSAGWHHTCALLANREVLCWGSNVFGQLARVEGARALHA
                     TSSGFDDVAALAAGGQHTCVVRMDRATFCVGREDAGTGEHDPQAR"
     gene            complement(4996..6204)
                     /locus_tag="AC16BC_00335"
     CDS             complement(4996..6204)
                     /locus_tag="AC16BC_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3240085.1"
                     /translation="MDPLHNPFSPGAGSPPPALAGREQILEQARITLGRIKAGRPERS
                     QILIGLRGVGKTVLLNRIRELAEQSDYKTVFIEAHENKPLVALLLPPLRQLLISLDRM
                     ASVSEKAKRGLRVLRSFFNGVKIKYQDLEIGIDIDPEPGAADSGDLEADLSVVFLAVG
                     EAAADRGAAIALIIDELQYLNEEELSALIMAMHQISQRQLPVVLIGAGLPQLVGLSGR
                     AKSYAERMFQFPELGPLQPADAAAALQAPVMEQRVRFTDEALKEIIRQTQGYPYFLQE
                     WGYQAWNLAAKSPIGIDVVKQATRESIQRLDANFFRVRFDRLTPREKEYLQALAELGP
                     GPHRSGDVAEQLGVKVQSVAPVRNELIKKGMIYSPAHGDTAFTVPLFDDFMTRIMPRA
                     GSRKAGKKSS"
     gene            complement(6402..7460)
                     /locus_tag="AC16BC_00340"
     CDS             complement(6402..7460)
                     /locus_tag="AC16BC_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HIR3240086.1"
                     /translation="MLFDPFTLREVTFRNRIVVSPMCQYSYEDGFSNDWQLVHLGSRA
                     VGGAGLVFAEAAAVEAQGRISPGDLGIWKDGHVEPLRRVARFIASQGAVPGIQLAHAG
                     RKASTGIPWITDKPLGEQEGGWKPVGPSAVPFADGYATPHVLTREDILQIVTAFGAAA
                     RRARQAGFQVVEVHAAHGYLINEFLSPFSNKREDEYGGSFDNRTRLLREVVKAVRAEW
                     PEGFPLFCRLSATEWKEGGWTIEDSVALAKILKNEGVDLMDCSSGGNVPGVAIDLVPG
                     YQVPLAERVRKDAGIPTGAVGLITSARQADQIVTSGQADLVFLARQMLRDPYFPLHAA
                     KELGVEIKWPNPYLRASR"
     gene            complement(7563..7922)
                     /locus_tag="AC16BC_00345"
     CDS             complement(7563..7922)
                     /locus_tag="AC16BC_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240087.1"
                     /translation="MTNAMKGVLLSGLVFPGLGQMALKRYQRGVVFILASVAGLVVIV
                     AQAAREAGRILDAIEAGGGVIDEDAMSRAIGQATAWSGNLTFNLLLVALTLCWIYAAV
                     DAYRIGKQRDRHKESGA"
     gene            complement(7973..8230)
                     /locus_tag="AC16BC_00350"
     CDS             complement(7973..8230)
                     /locus_tag="AC16BC_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017710790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HIR3240088.1"
                     /translation="MFDPVSALGFVAGMFTTVAFIPQLVKARRSQSTKDLSLPMFLIF
                     AAGVLLWLVYGIAIRSMPVIVANGVTLVLAGWILLLKIRLG"
BASE COUNT         1426 a         2922 c         2604 g         1403 t
ORIGIN      
        1 tccccccgcc gaacctccaa cgacgcgcgc tccagcaagg gacgcggtcc gtaggcgagc
       61 gaaacgttat caaggcgaat caacggcatg aaatccctta ctctcgtgac aaggtttacg
      121 agggaaaacg agttcagcag gtggacgaca gacggacgct gaaatctgca agtgcaagtc
      181 cttcgacagg ctcaggacga acggatccgg atcgtgacat gtcagtgagt ttccgttcgt
      241 ggtgagattg tcgaaccacg ctcaatattc tttcaacgcc ctaccgcacc ggaacccgcg
      301 tcaccgcgcc ttcggacgcg gagccgacca tgcccgcgta tttggcgaac acgccggtct
      361 tgtagcgcgg ctcaggggct ttccatgact tcaagcgatt ctttaactcg tcgtcggaga
      421 cctcgacgtc caggcgtcgt ttggtgatgt cgaacgtgat catgtcgcct tcgcgcaccg
      481 cggcgatggg gccgcccgcc gcggcttccg gcgccacgtg cccggccatc aaccctttcg
      541 ttgctccgga gaaccggccg tcggtgagca aagctacact gttgcccaag ccctcgccca
      601 cgatcgcagc ggtcacgccc agcatctcgc gcatgccggg accgcccttg gggccttcgt
      661 agcggatcac gaccacgtcg ttctcgcgga tctggcgttt ggtcacggca gccatggcgt
      721 cttcctcgcg gtcgaacacg cgggccgggc cgcgatgcag caggcgctcg tggcccgcga
      781 ctttgaccac gcagccctcc ggcgcaaggc tgcccttgag aatcacgagc cctccggtcg
      841 gtttgagcgg cttgtcgacc ggagtgagca ccacctggcc aggcgtctcc accgcgccgg
      901 cggattcttc cgcaatcgtg cggccggtcg gcgtcagttg tgtgccgtcc aggaacccgg
      961 ccttcaccag gcgttgcgcc accaagggga tgccgcccgc gcgatccaga tccgcggcca
     1021 catatttccc gccgggcttc aaatccgcga tcagcggcgt gcgggtgctg accacgtcaa
     1081 aatcgtcaat ggtgagcgga atccctgcct cacgcgccat ggccaagagg tgcagcacgg
     1141 cattcgtgga gccgccggtg gcggcgaccg aggcgatcgc gttctcgaac gccttgcggg
     1201 tgaggatctt gctcggccgc agatcgcgct ggagcatgtc cattacggtt tgaccgactc
     1261 ggattccgac cttctccttg cgcggatccg tggcgggcac gctcgccgta ttcatcaccg
     1321 acaagccgat gaactccatg accgcggcca tggtattggc cgtgaactgc ccgccgcacg
     1381 cgcccgcgcc gggacaggcc gagtcttcga gcagtttgag gtccgcgtcg ctcatgcggc
     1441 ccgccgcgtt cgcgcccacg ccctcgaaca cgtcctggat ggtcacgtcc ttgccgtgaa
     1501 agtgtccggg ctggatcgac ccgccgtaga gcacgatgcc tggcgtgtcc aagcggatca
     1561 aggccatggc gccggccgga atggtcttgt cgcaccccac caggacgatc atggcgtcga
     1621 acagataccc gcgccccatc agctcgatcg aatccgcgat cgcttcgcgg ctgatgagcg
     1681 aggccttcat gccctcggtg cccatggtca cgccgtcgga cacggacacg gtgttgaact
     1741 ccatgggcgt gccgcccgcc tcgcgaatcc cgcgtttgac gtggaccgcc agatcgcgca
     1801 ggccgtagtt gcacggcatg gtctcggtcc aggtgttggc gatgcccacg atggggcgcg
     1861 ccagatcctc gtcggtgaag ccgatgcctt tgagcatggc gcgagccggc gcgcgatcgc
     1921 ggccgttggt gatggcgcgg ctgcgggact tgggatcaac ggacatgggt gcaaactcct
     1981 tggtcagggc gtgagatgag acaatcaacg tgaggggatc ataccaaagc agcacgcgcc
     2041 gtggaagggg cggaagcccg aggcgcggac tgcgcgacgg tgaccgacag agccacgaag
     2101 ccgccgctgc agcagacggc tcatccatag tcggacctac gtcagccggc cgcgcttttc
     2161 ccgtcgaatc tcgtggatct cctcggagat ctcctccggg gacatctgcc ggcccccgac
     2221 gggtttctta acccgcgacg tcttacgcgc ggccagtcgc tgcagcgtga tggtgtcgtc
     2281 ttcccacgac accgccacgt tgcttccggg cggaaacgtc cgcagcacgt ccgccggtaa
     2341 tttgatcgag ccatcgcccc gaattttcac cgtcttcatc cccgtccctc cagtggcaac
     2401 cgtcacccct cctcgatcat gtgcgcaccc atgcccgtga tccgggcggt ctcgcggccc
     2461 gggctcacgg gattcgcccg gatttcatgg tgttgaccgt gaacacgcgg tcgttcaatc
     2521 cggtgttcac ggtcacgttg cgcagttcca ccacggtctg gcccggggga tccgcggcgc
     2581 gcacctccac ccgcttccac acccaggcgc ggccgacccg ttgccacgcg atcgattggg
     2641 tcacgtccac ccgtccgccc gcgccgcggt actcgatctt cgcgatggtc caattcacgg
     2701 gcgacaccca gaaccgccgg agcgcgtcat tgtccccgtc gaggcgctcc tcgatcacga
     2761 ggtgatccac gccgtcgagc ggctcgtggc cagccaccga ccgagcggcc tttcccaaca
     2821 gcctcgcgcc caactgctcg aatccgagca acagatccga gttgcgcttg gggcgttgcg
     2881 tgtcctgccc ggcaatggtg atccggagcg cctggcggag gtccggcaga tagagccacg
     2941 cctgcgaatc cccggcgcgt tccgcctgcc ggaggagaaa actctcgccc ttgcggttca
     3001 acggcgcctg cgtgaccagc agcgtcagcc cgatcaggcc gtctcgcccg cgctggttct
     3061 tccaatagag tctgagcgtc gtgctccgcg cgtccccttg cgctcccgtg gaccggattt
     3121 ccacgtccgc ggtttcgtcg tcgatggcgt cgtcctgggc cctgaccagc cgcacgatgt
     3181 catcgacgct cggctcctcg ccccgcccga tcgcgggcgc gacgaggacc gcgatcatca
     3241 tcaacgcgac cataccgagc gcccgcgtca ccagaacgca atgcgtctcc cacagcatgg
     3301 aacgggtcac tcgaccggcg agcgttcggc gagcgcgtcg gggatcaatc gccgcccttc
     3361 agggtgtccg cgtcgtaatc gagcgggtcg tcgactttct cgcccaggcc ggcgcgaaac
     3421 gactgctcca ttgcgcgcag acgcgcgagg cgctcggcgt cgtggtccga ttcgcgctgg
     3481 agcagggccc ggcacaaggc cagcccgcgc cggaaatgct ccgccaccgt ctcgtccatc
     3541 aagtggctcc acgtggtgta caccaggaac tctccgaacg ccgaagcctc cggatgccgc
     3601 gcgccgatgg ccaccaactc gcggtacgcg tcagaggcct cgtcgcccgc gccgccggag
     3661 aagcgttcct gggcgtcgat cgccgcgtcc agatcctcgc ccaactcgtt ggacgcccgc
     3721 acgcgacaaa acacctgcca ggccacgatg gccggatcaa agttcatgac ggcttcctct
     3781 cacgccgggc atctcatcgc gcctggggat cgtgttcgcc ggtgcccgcg tcctcgcggc
     3841 ccacgcaaaa cgtggcgcgg tccattcgca cgacgcacgt gtgctgcccg cccgcggcca
     3901 gagccgccac gtcgtcgaac ccggacgagg ttgcgtgcag agcccgcgcg ccttcgaccc
     3961 gcgccagttg cccgaagacg ttgctgcccc agcacaacac ctcccggttg gccagcaacg
     4021 cgcacgtgtg gtgccacccc gctgagaggc gctccacgtc cgccaagggt acggcggtcc
     4081 cgttcgcatc caccgtggcc accggcgccg gcgtgagcgc gcgcgtggtg gtgccgttgc
     4141 cgacctggcc ctcgacattg tccccccaac actgcaccgt caggtcgcga agccgcgcgc
     4201 aggtgtgccg cgtgcccgcc accacctgcg ccacctcgct ctgaatcgcc acgggctgag
     4261 ggggaaacga gttgtggcgt tgaatcccgt gaccgagctg gccgtaatga ttgtcacccc
     4321 agcagaacag gcgcccgtcc gcgaccgccg cgcacgagtg ttcattgccc gccgcgatcg
     4381 acaccgcgcg ttcaatgccg ggcactttga ccggggcgga cgaggtttgc cgcggcgggg
     4441 cctgcacccc gagctgatcg tccttgttcc cgccccaaca ccagaccgag ccgtccgcga
     4501 gccgcgcgca ggtgtggctg tgcgccgcgg cgagttccac cgccggaccg acggccacgg
     4561 acacgggccg cgacgaccga cgcggcttgg ccgtgccgag ttgcatcaaa tcgttcttgc
     4621 cccagcacac caccgcgccg gccttcacga tggcgcaggt atgccagtct ccggccgcca
     4681 gccccgtcgc gcccgcgacc ccggccaccg ccaccggcga cgcggaccga ccgtcgcccc
     4741 cgtcgcccaa ttgccagaag ccattatctc cccaacacgc gaccgtggag tcgggctcga
     4801 tgcggcaggt gtgcgagcct ccggacgcga tcacatcggg accacgtgtc gccgacgggt
     4861 cgggctcggc ggccaaaccg gccgatcccg ccatcaacac aacgaccgca acacccccga
     4921 gcacccggat catcgcacgc cctttctgcc gcggtcgccc tcccgccgcg aagacgctca
     4981 acacccggcg agcggtcacg acgacttttt ccccgctttc cgcgagccgg cgcgcggcat
     5041 gatgcgcgtc atgaagtcgt cgaacagggg caccgtgaac gcggtgtccc cgtgcgcggg
     5101 gctgtagatc atgccttttt tgatcaactc gttgcggacc ggcgccacgc tctggacctt
     5161 gacgcccaac tgctcggcca cgtccccgga acgatgcggc ccggggccca gttccgccag
     5221 cgcctgcaga tactcctttt cccgcggggt cagccgatcg aaccgcacgc ggaagaagtt
     5281 ggcgtccagc cgctgaatgg actcgcgggt ggcctgcttg accacatcga tgccgatcgg
     5341 cgacttggcg gccaggttcc acgcctgata gccccactcc tgcaagaaat acggataccc
     5401 ctgcgtctgg cggatgattt ccttgagcgc ctcgtcggta aaccggacgc gttgctccat
     5461 gaccggcgcc tgcagggcgg cggccgcgtc ggccggctgc agcggaccga gctcgggaaa
     5521 ctggaacatg cgctcggcgt acgacttggc gcggcccgag aggcccacga gttgcggcaa
     5581 gcccgcgccg atcagcacca ccggcaactg ccgctgcgag atctggtgca tggccatgat
     5641 cagcgcggac agctcctctt cgttgaggta ctgcagttcg tcgatgatca acgcgatcgc
     5701 ggcgccgcga tccgcggctg cctccccgac cgccaggaag accacggaga gatcggcctc
     5761 gaggtcgccg ctgtcggccg cgccgggttc gggatcgatg tcgatgccga tttccagatc
     5821 ctgatacttg atcttcacgc cgttgaaaaa gctcctgagc acgcgcagcc cgcgtttggc
     5881 tttttcgctg acgctggcca tgcggtccag ggaaatgagc aattgccgca gcggcggcaa
     5941 caagagcgcg accaaaggct tgttctcgtg cgcctcgatg aagacggtct tgtagtcgga
     6001 ctgctccgcc agttcgcgga tcctgttcag cagcacggtc ttgcccacgc cgcggagccc
     6061 gatcaggatc tggctgcgtt cggggcggcc ggccttgatg cggcccagcg tgatccgagc
     6121 ctgctccaaa atctgctcgc ggccggccag cgccggcggc ggcgatcccg cgcccggcga
     6181 gaaggggtta tgtaaggggt ccatcgatag cgtggttacg aaagttacca atgtatagca
     6241 taaaaatgat aacttcacat aacttcccta tacacgaggg acggcggact cgagactcct
     6301 ggaacccgcg gccggagaaa cgagtggcgg cggggacaag gcgggaggac tgaggcggaa
     6361 cggactttga atcttaaaag aacgtcccca aaggtcatgg actatcggct cgcccgtaga
     6421 tatggattgg gccatttgat ctccaccccg agttccttgg ccgcgtgcaa cgggaagtac
     6481 gggtcccgaa gcatctggcg ggccaggaag accaggtccg cctggccgga ggtcacgatc
     6541 tggtccgctt gtcgggcgga cgtgatgagc ccgaccgcgc ccgtggggat gcccgcgtcc
     6601 ttcctcacac gctccgccag gggaacctgg tacccgggga ccagatcgat cgccacgcca
     6661 gggacattgc cgccgctgga acagtccatg agatcgacgc cttcgttctt gaggatcttg
     6721 gccaatgcca cggaatcctc gatggtccac ccgccctctt tccattccgt ggcggacagg
     6781 cggcaaaaca gggggaaccc ttccggccac tcggcccgca cggccttgac cacctcccgc
     6841 aacaatcgtg tccggttgtc gaaggatccg ccgtattcgt cctcccgttt attcgaaaag
     6901 ggagacagga attcgttgat caggtaccca tgggccgcgt ggacctccac cacctggaat
     6961 ccggcttgac gcgcccgtcg ggccgcggcc ccgaaggccg tgacgatctg gaggatgtcc
     7021 tccctggtca acacgtgggg cgtggcatag ccgtccgcga acgggaccgc actgggcccg
     7081 accggcttcc acccgccttc ctgttccccc aggggtttgt ccgtgatcca cggaatgccg
     7141 gtggacgcct tgcgacccgc gtgcgccaac tggatgccgg ggacggcgcc ctgcgaggcg
     7201 atgaaccggg cgacgcgtcg gagcggctcg acgtgcccgt ctttccagat gcccagatcg
     7261 ccggggctga tccggccctg ggcctccacc gcggcggctt cggcaaacac cagccccgcc
     7321 ccgccaacgg cccggctccc caggtggacc agttgccagt cgttggaaaa accgtcctcg
     7381 taggaatatt ggcacatggg cgacacgacg atccggttgc ggaacgtgac ctctcgcaag
     7441 gtgaacgggt caaacagcat tacaacctcc gaatgtgtcc gatgccaggg gaatgtgagt
     7501 cagagtcgtt gttcatggaa gaatacgctc ggcgccacgc gcaaaaagga ctttgacgcc
     7561 aattatgctc ccgactcttt gtgtcggtcc cgctgtttgc cgatgcggta ggcgtcaacc
     7621 gccgcgtaga tccagcagag cgtgagcgcg acgaggagaa ggttgaaggt gaggttgccg
     7681 gaccaggccg tggcctggcc gatggcgcgc gacatcgcgt cctcgtcaat gacgccgcct
     7741 cccgcttcaa tcgcgtcaag aattctcccg gcctcgcgcg cggcttgggc cacaatcacc
     7801 accaggccgg ccacgctcgc caggatgaat acgaccccgc gctgataccg tttgagcgcc
     7861 atctgtccca ggcccgggaa gaccagcccc gagagcaaca cgcctttcat cgcgttcgtc
     7921 atcagttccc gtccccctca gtcgcgtccg cgttgcggcg cggttccccg aatcacccga
     7981 ggcgaatctt gagcagcagg atccacccgg ccagcaccag cgtgacgccg ttggcaacga
     8041 tcaccggcat cgagcgaatc gcgatgccat agacgagcca cagcaggacg ccggcggcaa
     8101 agatcaagaa catgggaagc gagaggtcct tggtggattg ggaacgcctc gccttgacca
     8161 actgggggat aaacgcgacc gtcgtgaaca tcccggccac gaaccccagc gcggagaccg
     8221 gatcaaacat tgcggcgcaa ccgccgggtc atgagtcggc ttctcatcgt gtttcgcgaa
     8281 aaatccggga cgtcccgctc ccctcagtcc cccggccccg gggcgcggtc gggcgctgac
     8341 tcgtcacggg cggtc
//
LOCUS       DBNGTT010000017         3868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_642255, whole genome shotgun sequence.
ACCESSION   DBNGTT010000017 DBNGTT010000000
VERSION     DBNGTT010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_642255"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1412
                     /locus_tag="AC16BC_00355"
     CDS             288..1412
                     /locus_tag="AC16BC_00355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3240089.1"
                     /translation="MPETPLLIIAASESDSNLYYATRFLAPDAFVYLRDRDQSMLLMS
                     DLEMDRAKAQAKVDTVLSYTTYEERAKAKMSDRPRVVHVVRELLNERGIKAFDVPASF
                     PIEYGDMFRGMGFTVGVRPDPLFPERVLKQPQEVEAISATQRAVEEAVAAAMQLLRDA
                     DVRNGLLYADGAALTSERIKQLINVKLMERDCVAQHTIVACGVDACDPHNEGSGPLLA
                     NQPIIFDVFPRSSHSRYFADMSRTVVKGRASDGMKRLYEAVLEGQEWGAAQVKAGVDG
                     QSIHQGICDRFKAKGYETGLKDGRMQGFFHGTGHGVGIDIHEAPRVSKVSHVLQPGEV
                     VTIEPGLYYPAIGAARIEDMVLVTADGGVNLTRFPKTLEL"
     gene            1700..2164
                     /locus_tag="AC16BC_00360"
     CDS             1700..2164
                     /locus_tag="AC16BC_00360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3240090.1"
                     /translation="MPRPNKTVADSASEMLQVVLPNDTNPLGNVLGGRVMHWIDLVGA
                     IVAYRHCHGPVVTASVDRLDFRHPIKLGEIAVLKARLVYVGRTSMEVLVDVFVDDPPS
                     GTRNQTSAARVTYVAVDKRGRPVVVPSLLLASDDERRLFEEALARRRSEGRA"
     gene            2222..3172
                     /locus_tag="AC16BC_00365"
     CDS             2222..3172
                     /locus_tag="AC16BC_00365"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR3240091.1"
                     /translation="MRTTFHIQKFNPETDAGPRMCEYHLDVAWKGNTVLDALFQIKNE
                     LDGSLTFRASCRSAICGSCPMVINGRERLACQTLIAGEIEQHGRIEVRPLRQMPVIKD
                     LAVDLTPHWEKVRAITPFLTTQAHRPHTWNSHQDPHGGFQNVDACILCGACVSACSSL
                     EVSPGFLGPAALAKAYRFVADPREGHAKSRLENLTQENGIWDCVRCNWCVEVCPKDVK
                     PMEAIIRLRREAIHAGLTDHPGATHITAFMDLVKHEGRLNEARMPVAMVWPKLGRLLG
                     VVPLGIKMFLKGKVPAWFQRPIPGIREVRRLFEVREASAK"
     gene            3169..>3868
                     /locus_tag="AC16BC_00370"
     CDS             3169..>3868
                     /locus_tag="AC16BC_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR3240092.1"
                     /translation="MKYAFFTGCAAKGACPELYQSAVKVAGLLGIELEEMKSAACCGA
                     GVVREGDAELSLAVNARTFAQAEAKGLPVLTICGTCQGVLAGANKTLKEDAAALERIN
                     ALLAPDGLTYRGTTEVKHLLWVLIRDVGLATLRKAVKRSLSGLRVAPFYGCYILRPSS
                     DLGFDDPSNPSSMEELIIALGGQRVDYGSRVKCCGFPLVLENEPRALAMSGNATADAK
                     REGADCMVTPCPLCH"
BASE COUNT          694 a         1187 c         1312 g          675 t
ORIGIN      
        1 gacgacgatc tgccgggtga gctcgatgcc cctgaacaca cgtacgtcgg catgatgagg
       61 gaaaacgtgg agaccatggc gcgggtgttg ggcggggatg cttcggcgct gaagaacatt
      121 gaccccagca atgtagggaa atagagcgcc tcgaaaggtc ggctccgacg ggaaaaccat
      181 ccagcgagcc cacgatactt ggggcgtcca tcgctggtct tgcgtcggac ctctgtgcca
      241 gcggtcggct agacgtgcca tgtccgctgt gttagattgt gagcccgatg cctgagaccc
      301 cgctgctcat catcgccgcg agcgagagcg actccaatct gtattacgcg acgcggtttc
      361 tcgcgcccga cgccttcgtg tatttgcgcg atcgggacca atccatgctc ctgatgagcg
      421 acctggagat ggatcgcgcg aaggcgcagg ccaaggtcga caccgtgttg tcgtacacca
      481 cgtacgagga gcgggccaag gccaagatga gcgaccggcc gcgcgtggtg cacgtggtcc
      541 gcgaactctt gaacgagcgc gggatcaagg cgttcgatgt gcccgcgagt ttcccgatcg
      601 aatacggcga catgttccgc gggatgggct tcacggtggg ggtgcgtccc gacccgttgt
      661 tcccggagcg cgtgctcaaa cagccgcagg aggtcgaggc gatttccgcc acccagcgcg
      721 cggttgaaga agccgtggcg gccgcgatgc aactgttgcg ggacgcggac gtgcgcaacg
      781 gcctattgta cgcggacggc gcggcgctca cctccgagcg catcaagcaa ctcatcaacg
      841 tcaaactcat ggaacgcgac tgcgtggccc agcacacgat cgtggcgtgc ggggtcgacg
      901 cgtgtgatcc gcacaacgaa ggctcgggtc cgttgctggc gaaccagccc attatcttcg
      961 acgtgttccc ccgatccagc cacagccggt atttcgcgga tatgagccgc accgtggtca
     1021 aaggccgcgc gagcgacggc atgaagcggt tgtacgaggc cgtgctcgag gggcaggagt
     1081 ggggcgcggc gcaggtcaaa gccggcgtcg acggtcagtc gatccatcag ggcatttgcg
     1141 accggttcaa ggccaagggc tacgagaccg gtttgaagga cggccgcatg cagggattct
     1201 ttcacggcac cggccacggg gtagggatcg acatccatga agccccgcgc gtgagcaagg
     1261 tcagccacgt gttgcagccg ggggaggtcg tcacgatcga gccagggctg tattatcccg
     1321 ccatcggcgc ggcgcgcatc gaggacatgg tgctcgtgac cgcggacggc ggggtcaatc
     1381 tcactcggtt ccccaagacc ctcgaacttt aggagcctgt ccatgagcgg ccatctgcgg
     1441 cgttgtcgct gcgcttgctt cgccaagcga gcggaacgga tcgttgacag cgccatacta
     1501 cggcgcagtg cgctttccgg tgctccatca gctagtagat accgggctca ccgattccag
     1561 tggcctgact ccctccggtt ctcgctccgc cttgcgacct accgacagga atccaaatgg
     1621 cggaagtccc cgaagggggc atctggacca ctcctgaaca ggctctggcc gacgatttgt
     1681 caatacgagt ctcagcgaag tgcccaggcc caataagacg gtcgcggatt ccgcgtcgga
     1741 gatgttgcag gtcgtgttgc ccaacgacac caatccgctt ggcaacgtgc tgggtggacg
     1801 cgtcatgcac tggatcgact tggtcggcgc gatcgtggcg taccggcact gccacggacc
     1861 ggtggtgacc gcctcggtcg accgcctgga cttccggcat cccatcaaac tcggggaaat
     1921 cgccgttctc aaggcccgcc tggtgtacgt cggccggacc tcaatggaag tgctggtcga
     1981 cgtgttcgtg gacgaccctc cgagcggcac gcgcaatcag accagcgccg cgcgcgtcac
     2041 gtacgtggcc gtggacaagc gcggtcggcc cgtggtggtg ccgagcctgc tgctggcgag
     2101 cgacgacgag cgccgtctgt tcgaggaggc gcttgcccgt cgtcgctcgg agggtcgagc
     2161 ataacgcccg ttgtgacgca ccgttttttg cgttctcggc tatcctgtac cgggtaacgc
     2221 gatgcgcacc accttccaca tacagaaatt caatcccgag acggacgccg gaccgcgcat
     2281 gtgcgagtat cacctcgacg tggcgtggaa gggcaacacc gtgctcgacg ccctgttcca
     2341 gatcaagaac gagctggacg gctcgctcac gtttcgggcg tcgtgtcgta gcgcgatctg
     2401 cgggtcgtgt ccgatggtga tcaacggccg cgagcggctg gcgtgccaga ccttgatcgc
     2461 cggcgagatc gaacagcacg gccggatcga ggtgcggccg ctccggcaaa tgcccgtcat
     2521 caaagacctg gcggtggatc tgaccccgca ctgggagaag gtccgcgcga tcacgccgtt
     2581 cctcacgact caggcgcatc gaccgcacac gtggaacagc catcaggacc cgcacggcgg
     2641 gtttcagaac gtggacgcgt gtatcctgtg cggcgcgtgc gtgtccgcct gttcgagcct
     2701 ggaagtgtct ccgggatttc tcggaccggc ggcgttggcg aaggcgtatc gatttgtcgc
     2761 ggacccgcgc gagggtcatg ccaagtctag gctggagaac ctcacgcagg agaacggaat
     2821 ctgggattgc gtgcgctgta attggtgcgt tgaagtgtgc cccaaggacg tcaagcccat
     2881 ggaggcgatc atccggctgc ggcgcgaggc gattcatgcc gggttgaccg atcaccccgg
     2941 cgccacgcac atcaccgcgt tcatggatct ggtcaaacac gaggggcggt tgaacgaggc
     3001 gcgcatgccg gtggctatgg tgtggcccaa gctggggcgg ttgctgggcg tcgtgccgct
     3061 cgggatcaag atgttcctca agggcaaggt gccggcgtgg tttcagcggc cgatcccggg
     3121 catccgcgag gtgcgccgat tgttcgaggt cagggaggcc tccgccaaat gaagtacgcc
     3181 ttcttcacgg gctgcgcggc caaaggcgcg tgccccgagt tgtaccagtc ggcggtgaag
     3241 gtcgccggcc tgctgggcat tgaactcgaa gagatgaaga gcgcggcgtg ctgcggcgcg
     3301 ggcgtggtcc gcgaaggcga cgccgaactc agcctagccg tgaatgcgcg cacgttcgcg
     3361 caggccgaag ccaagggcct gcccgtgctc accatctgcg gcacgtgcca gggggtgctg
     3421 gcgggtgcga acaagacgct caaggaagac gcggccgcgc tggaacggat caacgcgctg
     3481 ctggcgccgg acggcctcac gtaccgcggc accacggagg tcaaacacct gctgtgggtc
     3541 ttgatccgtg acgtgggcct ggccacgttg cgcaaggccg tgaaacgctc gctctcgggc
     3601 cttcgcgtgg cgccgttcta cggctgttac atcctgcgac cctcgtcgga tctcgggttc
     3661 gacgatccca gcaatccgag ttcaatggaa gagctgatca tcgcgctcgg cgggcaacgc
     3721 gtggattacg gcagccgggt caagtgctgc ggtttcccct tggtgctgga gaacgagccc
     3781 agggcattgg ccatgagcgg caacgccacc gcggacgcca agcgcgaggg cgccgactgc
     3841 atggtcacgc cgtgcccgct ctgccaca
//
LOCUS       DBNGTT010000018         3159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1791973, whole genome shotgun sequence.
ACCESSION   DBNGTT010000018 DBNGTT010000000
VERSION     DBNGTT010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1791973"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..534)
                     /locus_tag="AC16BC_00375"
     CDS             complement(169..534)
                     /locus_tag="AC16BC_00375"
                     /inference="COORDINATES: protein motif:HMM:NF020950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HIR3240093.1"
                     /translation="MGSPVISSEAATKTIDATAGEPFHVALWEDRTAGYSWTPRYDAN
                     AFDLIDDDYQRTVNVDTADFGRRTFTFLAKTPGEHPLVLENRVGWKFSASNRKQYVVN
                     IHPATASAHPPSIDDTAAC"
     gene            659..1450
                     /locus_tag="AC16BC_00380"
     CDS             659..1450
                     /locus_tag="AC16BC_00380"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3240094.1"
                     /translation="MGLLDGKSGVIMGVASERSIAWGIAQALHKEGARLAFTYAGDAM
                     ERRVRPLAESLGEKVVVPCDVSSDGEITRAFDTIGTEFGGVDVLIHSIAFANREDLKQ
                     RFVDTSREGFRVALDVSAYSLVATARAVRPLMTGRRGSIVTLSYYGAEKVMPNYNVMG
                     LAKAALEAGVRYLAADLGPEGIRVNAISAGPIKTLAAAGIAGFREILHYNESVAPLRR
                     NVTLEDVGGAALFLCSELASGVTGEVIYVDGGYHVIGMGIPTPAT"
     gene            1447..2382
                     /gene="serB"
                     /locus_tag="AC16BC_00385"
     CDS             1447..2382
                     /gene="serB"
                     /locus_tag="AC16BC_00385"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR3240095.1"
                     /translation="MSRPDAFVLIGLSTADPASALDAAAAALKAHGARVGEPRSVASR
                     RFHAVTREVSASGSSAWRDLTRALRPLQVRLGVDLAVVPADPARRAPKVLVMDMDSTL
                     VQSEGIDELAKEAGVGEQVAAITRRAMNGELDFRGALKERVGLLRGLSAEALATVHAR
                     TYPTHGAEILLDTLRKVGCALAVVSGGFDYFTERLKNRLGLDHALANRLEVEGGLLTG
                     RLVGDIVDGTRKAAMVDELARAYGVGLDRVVAIGDGANDLPMLGRAGLGVAFCAKPAV
                     RESAPVTVSVKDLSAVLCFLGYDEREFAHEAESRC"
     gene            2360..2983
                     /locus_tag="AC16BC_00390"
     CDS             2360..2983
                     /locus_tag="AC16BC_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3240096.1"
                     /translation="MKPKAVADVVRGYDAPLARLVEEYFRGNEAARVALDLAGRDGRP
                     FVIDHAAIRTHDVDERADEFRRLGFEYRDELVEFSDQGWWAKVYRKAGMPALFVDQAY
                     DDARGKKSLLSAWVDRFGDRVLHHVAIRVPDIDAAKAELERAGVQFSGAVVGPRGTRL
                     RQIFTAAEVRDGHPFTVLELAERNGYEGFVPEQADGLMQSSVVKKTA"
BASE COUNT          497 a          929 c         1135 g          598 t
ORIGIN      
        1 cggcggtgtg gtggtacgga ttgtttggtc tgcgttcatc gagtactccc tacgaaataa
       61 gaacgataat tcccgatatt gtccatgttc atgcggtcag ccgcgtgatc ccggacacgc
      121 cagcgtcggg ttgcgccttc ggcggcgtgc tgcacggccc caagtatttc agcaggcggc
      181 tgtatcgtca attgaggggg gatgagccga cgccgtcgcg gggtggatgt tgacgacgta
      241 ctgcttccgg ttggatgccg agaatttcca gccgactcga ttttcgagga cgagggggtg
      301 ttctccgggt gtcttggcca gaaacgtgaa cgtgcggcgg ccgaagtcgg ccgtatccac
      361 attgacggtc cgttgatagt catcatcgat cagatcgaac gcgttggcgt cataacgcgg
      421 cgtccagctg tatcccgcgg tccgatcctc ccacagcgcc acgtgaaacg gctcgccggc
      481 cgtcgcgtcg atcgtcttgg tcgctgcctc gctgctgatc acgggtgacc ccatctccag
      541 atccaggtta attgacgcgg atcataccac agacgccggg tcttcgtcac gttttgttgg
      601 ccgttatttg aaaacgtccg gggctttcgg tatgatcgcc gcggtttgga gaacgggtat
      661 ggggctgttg gacggtaaga gcggcgtcat catgggcgtc gcgagtgaac gcagcattgc
      721 atggggcatc gcacaggcct tgcataagga aggggcgcgg ctggcgttta cctatgcggg
      781 ggacgccatg gagcgccgcg tgcgacccct ggcggagtcg ctgggagaga aggtcgtggt
      841 cccatgcgac gtgtccagcg acggtgagat cacccgggcg tttgacacga tcgggaccga
      901 gttcggcggg gtggatgtcc tgatccactc gatcgcgttc gcgaaccgcg aggatctcaa
      961 acaacggttc gtggacacct cgcgggaagg ctttcgcgtg gcgctcgacg tgagcgcgta
     1021 ttcgctggtg gccaccgcgc gcgcggtgcg cccgctcatg accgggcgcc gcggcagcat
     1081 tgtgaccctg tcgtattacg gggcggagaa agtgatgccc aactacaatg tcatgggcct
     1141 ggccaaggcc gctttggaag ccggggttcg ttacctggcg gcggatttgg gaccggaggg
     1201 catcagggtc aacgcgattt ccgccggacc gatcaagacc ctggccgcgg ccgggatcgc
     1261 gggctttcgc gagatcctcc attacaacga atcggtcgcc ccgttgcgtc gcaacgtcac
     1321 attggaggac gtcggcggcg ccgcattgtt cttgtgcagc gagctggcgt ccggcgtcac
     1381 gggggaggtc atctacgtgg acggcggcta ccacgttata gggatgggga taccgacccc
     1441 agcaacgtga gtcgtcccga tgcgttcgtc ctcatcggcc tctcaacggc cgaccccgcg
     1501 tcagcgcttg acgcggccgc ggccgcgctc aaggcccacg gcgcgcgcgt gggtgagccg
     1561 cggtccgtgg ccagccgtcg tttccatgcc gtgacgcgcg aggtgtcggc gtccgggtct
     1621 tccgcgtggc gcgacctgac ccgcgcgctc cggccgttgc aggtccgttt gggggtggat
     1681 ttggcggtgg ttcccgcgga tcccgcccga cgcgctccga aagtgttggt catggatatg
     1741 gattctacgt tggtgcagag cgagggcatc gacgagctgg ccaaggaggc gggcgtgggc
     1801 gagcaggtgg ccgcgatcac ccgtcgggcg atgaacggcg agttggactt tcgcggcgcg
     1861 ctgaaagagc gggtcggcct gttgcggggc ctgtcggccg aggcgctcgc cacggtgcac
     1921 gcgcgcacgt atcccacgca cggcgccgag atcctcctgg acaccctgcg caaagtcggg
     1981 tgcgccttgg ccgtggtcag cggagggttc gattatttca cggagcgcct caagaaccgg
     2041 ctggggctgg atcacgcgct ggccaatcgt ctggaagtcg agggcggcct gttgaccggc
     2101 cggctggtcg gcgacatcgt ggacggcacg cgcaaagccg ccatggtcga cgagttggcg
     2161 cgcgcatacg gggtggggct cgatcgcgtg gtggcgatcg gcgacggggc gaacgacctc
     2221 ccgatgctgg ggcgcgcggg cctcggcgtg gccttttgcg ccaaacccgc ggtgcgcgag
     2281 tccgcgcccg tgaccgtgag cgtgaaggat ctgtcggccg tgttatgctt cctgggatat
     2341 gacgagcggg agttcgccca tgaagccgaa agccgttgct gatgtggttc gcgggtacga
     2401 cgcgcccctg gcgcgactgg tcgaagagta ttttcgcggc aacgaggccg cgcgggtggc
     2461 gctggatctg gccggccggg acggtcggcc gttcgtgatc gatcacgcgg cgatccgcac
     2521 ccatgacgtc gacgagcggg ccgacgagtt ccgccgcctg gggttcgagt accgcgacga
     2581 gttggtcgag ttttccgacc aagggtggtg ggccaaggtg tatcgcaagg ccggcatgcc
     2641 ggccttgttc gtcgatcaag cgtacgacga cgcgcgcggc aagaaaagcc tgctgtccgc
     2701 gtgggtggac cggttcgggg atcgcgtgct gcaccatgtc gccatccggg tgccggacat
     2761 cgacgccgcg aaagcggaac tcgagcgggc gggcgtccag ttttccggcg cggtggtggg
     2821 gccgcgcggc acgcggttgc gccagatttt cacggccgcg gaggtgcgcg acgggcatcc
     2881 gttcaccgtg ctcgaactgg ccgagcgcaa cgggtatgag gggtttgtgc cggagcaggc
     2941 ggatggcctc atgcagtcgt cggtggtcaa gaagacggcg tagagtctgt tcatcaggga
     3001 ccatctgcgg cgttgccggc tgtgcatccg atcctcacgt acgacccagt acgctccggt
     3061 tcggtgctcg ccggcgcctt gcatctggcc cgctgctgaa cagactccga ttggttccca
     3121 atcggaaaac ctcaagagca agtcggcaat catatatcg
//
LOCUS       DBNGTT010000019         2682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1408929, whole genome shotgun sequence.
ACCESSION   DBNGTT010000019 DBNGTT010000000
VERSION     DBNGTT010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1408929"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /gene="yidC"
                     /locus_tag="AC16BC_00395"
     CDS             <1..848
                     /gene="yidC"
                     /locus_tag="AC16BC_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR3240097.1"
                     /translation="SGTVVAERFGDKRVTIGLRESAPADGKPVAARLYAGPKEYDRLA
                     GLDIGLQETIDFGWFIYGSWGLVRLIAEPLFLLLQFLHSFTGNFGIAIILLTAMIKTV
                     FIPLSHKSFVSMKAMAALQPQTQALQKKFKHDKMRLNQELMALYKNNRVNPLGGCLPM
                     VVQIPFFIALFNILYTTIELRQAPFFGWITDLSDKDPWYVLPVLMGVTMFIQQKMQPT
                     TMDPMQAKLMLALPVIFTFFFLAFPAGLVLYWMTNNTLTILQQYVTLRYYKPQPLIIP
                     KGLST"
     gene            861..2255
                     /gene="mnmE"
                     /locus_tag="AC16BC_00400"
     CDS             861..2255
                     /gene="mnmE"
                     /locus_tag="AC16BC_00400"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR3240098.1"
                     /translation="MHGFSFPSDTICAIATAPGEAAIGVIRLSGPGAIAAVDRAVRLQ
                     SKQPLKSLEDRRVAMAQIIDESHSGIIDEVLVTVMRGPRSYTREDVVELSCHGGPHVL
                     KRVVELLLAPGVRLAQPGEFTRRAFLNGRIDLAQAEAVMDLIRAQSDAGARSAVAQLR
                     GGLSAEIRTLQERLLGVLVEIEASIDFSEEGLEFLPREALTASVDEALGEIRRLLATA
                     DQGRIVREGASVVIAGRPNVGKSSLLNVLLRENRAIVTPIPGTTRDTIEESANLGGIS
                     VRLSDTAGVRQTHDPVEVEGIRRTKDAIADADLVIAVFDGSEPLAEDDRALLGDLKGR
                     SVIAVLNKADLPQRLTPATITGVLSGAAVFQVSTLTRQGIEELTKEIVSRLHGGGGAK
                     EGAFVTRLRHVEALKRADASLVHLKVTLAECGPHECVALDLRGAVDALGEILGTMTTD
                     DVLNEIFSRFCIGK"
     gene            2299..>2682
                     /locus_tag="AC16BC_00405"
     CDS             2299..>2682
                     /locus_tag="AC16BC_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AlbA family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR3240099.1"
                     /translation="MTDNEFDEILSRGYERRGTEFKGPGSLSAKPLVAKVVRAVLGMA
                     NQQDGGLVVVGVDEDVKGKIKPVGLSESDLATWKYDDVAGAIAPYADPFVSFELETKE
                     YKGKRFVLFHVSEFDEIPVLCRKEYP"
BASE COUNT          531 a          746 c          852 g          553 t
ORIGIN      
        1 ccagcgggac cgtggtggcg gagcgattcg gcgacaaacg cgtgacgatc ggcctgcgtg
       61 aaagcgcacc tgccgacggg aagcccgtcg cagccaggtt gtacgcgggc cccaaggaat
      121 acgaccgact cgccggcctt gatatcggtc tgcaggagac catcgacttc ggctggttca
      181 tctacgggag ttggggcctc gtccgcctga tcgctgaacc gctgttcctg ctgttgcagt
      241 ttcttcatag cttcaccggc aacttcggga tcgcgatcat cttgttgacc gcaatgatca
      301 agaccgtgtt catcccgctg tcgcacaaga gcttcgtgtc gatgaaagcc atggccgcgt
      361 tgcagccgca gacgcaggca ctccagaaga aattcaagca cgacaagatg cggttgaacc
      421 aggagctgat ggcgctctac aagaacaaca gggtcaatcc gctcggcggg tgtttgccga
      481 tggtggtgca gatcccgttt ttcatcgcgt tgttcaatat cctctacacc accattgaat
      541 tgcgacaggc gccgttcttc gggtggatca cggatttgtc ggacaaggat ccgtggtacg
      601 tgttgccggt gttgatgggc gtgaccatgt tcatccagca gaagatgcag cccaccacca
      661 tggaccccat gcaggccaag ctgatgctgg cgctcccggt gatcttcacg tttttcttcc
      721 tggcgtttcc ggccggtctg gtgttgtact ggatgaccaa caacacgttg acgatcctgc
      781 aacagtacgt gaccctgcgg tactataagc cccagccctt gatcatcccc aaagggctgt
      841 ccacgtgacc gggggctttc atgcacggtt tttcgttccc atctgacacc atctgcgcga
      901 tcgcgaccgc gccaggagag gccgcgatcg gcgtgatccg cctctcaggg cccggggcca
      961 ttgccgcggt ggaccgcgca gtgcggctgc aatcaaaaca accgctcaag tcgctcgaag
     1021 accggcgcgt ggcgatggcg cagatcatcg acgaatccca tagtgggatc attgacgaag
     1081 tgttggtgac cgtgatgcgg ggccctcgga gctacacgcg cgaggacgtg gttgaactct
     1141 cgtgccacgg cggcccgcat gtgctgaagc gcgtcgtgga actgttgctc gcgcccgggg
     1201 tccggttggc gcagcccgga gaattcaccc ggcgcgcgtt cctcaacggc cgcatcgacc
     1261 tcgcgcaggc cgaggccgtg atggatctga ttcgcgcgca aagcgacgcg ggcgcgcgat
     1321 cggccgtggc gcaactgcgc ggcggtctgt ccgcggagat ccgcacgctg caagagcggc
     1381 tgctgggtgt gttggtggag atcgaggcga gcatcgactt ttccgaagaa ggtttggagt
     1441 tcttgccccg cgaagcgctc accgcgtcgg tggacgaagc tctgggcgag atccggcgct
     1501 tgctggccac tgccgatcag ggccgcatcg tgcgcgaagg cgcgtcagtg gtcatcgcgg
     1561 gccggcccaa cgtcggcaag tccagcttgc tcaacgtgct gctgcgcgag aaccgcgcga
     1621 tcgtcacgcc catccccggg accacgcggg acacgatcga agagagcgca aaccttggcg
     1681 gtatttcagt gcggctctct gatacggcgg gcgtgcgaca gacccacgat cccgtggaag
     1741 tcgaaggtat tcgacgcacg aaagacgcga tcgcagacgc ggacttggtc atcgcggtgt
     1801 tcgatggttc tgagccgctg gccgaggacg atcgcgcgtt gttgggagac ctcaaaggcc
     1861 gttcagtcat tgccgtgctg aacaaggcgg atctgccgca gcggttgact cccgcgacca
     1921 ttaccggtgt tctcagtggc gcggcggtgt tccaggtttc aaccctgacc cgtcaaggca
     1981 ttgaggaact gaccaaggag atcgtgtctc gtcttcacgg aggtggtggg gctaaggaag
     2041 gcgcgtttgt gaccaggctg cgccatgtcg aagcgctcaa gcgcgccgac gcctcgctcg
     2101 tccacctcaa agtaacattg gccgagtgcg gtccgcacga gtgcgtcgct ttggacctgc
     2161 gtggcgcagt ggatgccctc ggagagatct tggggaccat gacgacggac gatgtgctga
     2221 acgaaatctt ttccaggttc tgtataggga aatgattaga agctaagaga atagttctgt
     2281 agtctccgat tcaaggttat gacggataac gagttcgatg aaattctgag ccgtggttat
     2341 gagcgacgag ggaccgaatt caaagggccc ggctctctat ctgccaagcc gttagtcgca
     2401 aaggttgttc gtgctgtgct gggcatggcg aatcaacagg atggcggatt ggtggttgtt
     2461 ggagtcgatg aagatgtaaa ggggaaaatc aagcctgtcg ggttaagtga atcagacctt
     2521 gcgacctgga aatacgacga tgtggcggga gcaatagcgc cctacgcaga cccctttgtt
     2581 tcttttgagt tagagaccaa agaatacaaa gggaagcgat tcgttctttt ccatgtgagt
     2641 gagttcgatg agatcccggt tctttgcaga aaggaatatc cc
//
LOCUS       DBNGTT010000020         4087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1026036, whole genome shotgun sequence.
ACCESSION   DBNGTT010000020 DBNGTT010000000
VERSION     DBNGTT010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1026036"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="AC16BC_00410"
     CDS             complement(<1..658)
                     /locus_tag="AC16BC_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HIR3240100.1"
                     /translation="MAVTSQGGNDSGGIRETIGTYLNVIRRRRWYVVLPTVIISAVAI
                     PIALLWPATYKSTTLIMVEAQKIPQEFVRTTVTTPIEDRLKTISQQILSRTRLERVIQ
                     EFGLAQEYSGSQGGWFSRLWSRNQEPTEAGGTGISLDLVEGMKQNIEITVRAGNTFTV
                     SYSGSQPEAVMNVTNKLASLFIEENLRIREAQGEGTAEFLEGELANMKAKMEAQEEAL
                     R"
     gene            complement(1294..1890)
                     /locus_tag="AC16BC_00415"
     CDS             complement(1294..1890)
                     /locus_tag="AC16BC_00415"
                     /inference="COORDINATES: protein motif:HMM:NF046672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR3240101.1"
                     /translation="MGSRFVGTGVIAMIALLSACASRPVTNPVRAAAPPAPPAPIAEY
                     RIGPEDVLEIVVWKNADLSKTVTVRPDGIITLPLIGELRAGGLTASDVRAEIKSRLER
                     YKEVPEVSVTVADIRSYNLYIVGEVRTPGRYAVKSYTTLLQALALAGGFTEWADSDEI
                     VVVRRDAAAKEQRIRVRYKDLIRGGDFGLNPGDTVVVP"
     gene            complement(2155..3366)
                     /locus_tag="AC16BC_00420"
     CDS             complement(2155..3366)
                     /locus_tag="AC16BC_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3240102.1"
                     /translation="MAIETLFEPLQYEFFRNGLLAAAMTGAICGLIGVYIVLRNMSYI
                     GHGLSHSVFGGAVVSYVMSWNFYLGAGLWGFLSALLINSVAKRKKIGADAVIGIVTTA
                     SFAVGVVLISRVRKFTRNFEAALFGNILGVTADDLIVIAVVSLVSAALIFVFYKQLLF
                     TTFDPEVAKVYGVRTEWIDAGFALVLAATIVVSMQVLGVMLIVAAIVIPAATARLLTH
                     NFHRLVFTSIGLGVLCGVGGMYLSFLSNASSGATIVLFAATVFAAALAYDSFRRRAVR
                     AVSAPVVHPAQHEHSHQHGDILHQHEHPVKLDVEHSHHPAAASSAPAGTPASGGVNGA
                     DRVHVIIDGMTCAYCVRAVERALSRVDGVTAVRVDLAARSVDVTLDGTRPGGGERVRE
                     AIRSAGYQPLG"
     gene            complement(3344..>4087)
                     /locus_tag="AC16BC_00425"
     CDS             complement(3344..>4087)
                     /locus_tag="AC16BC_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR3240103.1"
                     /translation="PQGLYPGVVGPSGSGKTTLLKVLLGLVPVAAGDVSVAGQRIYGE
                     TARGVGYVPQIETVNWNFPVTVEQVVLMGRHREMGWLPWPSREDRKVALDLLEHLGIR
                     QYAHRQIRALSGGEQQRVFLARALISRPRLLILDEPTSGVDLKTQHHILHLLSQLSRE
                     GVTIILTTHDLNSVARHLPWVICFNRSIVAQGDPQEVFTSEILSRTYNSEMVVVRQNG
                     LLLIADSPNAGGHYDSRTVRAEDGDRDPI"
BASE COUNT          811 a         1344 c         1184 g          748 t
ORIGIN      
        1 cacggagcgc ctcctcctgc gcctccatct tggctttcat gttcgccaac tcgccctcca
       61 agaactcggc ggtcccctcg ccctgggcct cgcggattcg gaggttttcc tcgataaaca
      121 acgaggcgag tttgttggtc acgttcatca cggcttcggg ttgactcccg gagtaggaga
      181 cggtgaaggt attgccggcc cgaaccgtaa tctcgatgtt ctgcttcatc ccttcgacca
      241 gatccaacga aatgccggtg cctcccgcct ccgtgggttc ttggttccgt gaccacagcc
      301 gcgagaacca tccgccctga gagcccgagt actcttgagc cagtccgaac tcctggataa
      361 cgcgctccaa tctggtgcgg ctcaaaatct gttggctgat cgtcttgagc cggtcctcga
      421 tcggggtcgt cacggtggtc cgcacgaatt cttgcgggat cttttgcgct tcgaccatga
      481 tcagcgtcgt tgacttgtac gtcgcgggcc ataacaacgc gatcgggatc gcgaccgccg
      541 atataatgac ggtcggcagc accacgtacc accgacgacg cctgatgacg ttcagatagg
      601 tcccgatggt ctctcggatc ccccccgaat cgtttcctcc ttgacttgtt accgccatgt
      661 tcgctccacc cgtcagtctg acgaattccg cgcgccggcc ctgaacacgt cccgcgtccc
      721 gggaacgccc cgtctgccgc gcagagaatt ctggtaaatg gacaatcccc gcctttgatt
      781 attcgcccca accaacgata ccggttcgat gtccgcgttc cggcgcgcac acttacgaat
      841 tcgtggcccc atcaagcggg acgcaatcgg ttgcggcttc cggacatcgg ctcgcttcgc
      901 gttatgtcca agattgttcg cccacccgtt catgccgtca cgcgcgcaca cctgacaccg
      961 ttggctcaat accgtcgtcc cgagtcgtcc gcgcccctgc tctccagaga taccgcccgc
     1021 gcgagttccg gacgaactcc ggaaagccgg tgatagcgca acgacgtcac gcggcccatt
     1081 ccttggctct ctcaacggca aaaggtgtga cgccagactg gtcgcaataa cgctggtcac
     1141 accgtactgc acgaaagccg cccgggtcga agcgagcttt gatgatgaac cggaggccca
     1201 aaattccggt tcaagcttgc gggaaaatcc ctgtacgatc cgtcagtcgg cggtcaaacc
     1261 gggcgcccgc tcgccctcgg tccgcccgct cggttacggc acaacgaccg tgtcgccggg
     1321 gttcaggccg aaatccccgc cgcggatgag gtctttgtat cggacgcgga tgcgctgctc
     1381 tttcgccgcc gcatctcgtc gaaccacgac aatttcgtcg gaatcagccc attccgtgaa
     1441 gccgccggcc agcgcaagcg cttgcagcaa cgtcgtgtag ctcttgacgg cataacgccc
     1501 cggcgttcgg acctccccga cgatatacag gttatagctg cgaatgtccg cgaccgtcac
     1561 cgacacttcg ggcacttctt tataacgttc aagtcggctt ttgatttcag cgcggacatc
     1621 ggacgcggtc agcccgccgg cgcgaagttc gccgatcaac ggcagcgtga tgatgccgtc
     1681 cggccgaacc gtgacggttt tggacaaatc cgcgttcttc cataccacga tttccaagac
     1741 gtcttcaggg ccgatgcgat actccgcgat cggagccggg ggggcgggcg gcgccgcggc
     1801 acgaaccgga ttggtcacgg gacgcgaggc gcaggcgctc aacaacgcga tcatggcaat
     1861 caccccggta ccgacgaatc gagaccccat atgttctcta gactccgcgg ccctttccac
     1921 gaccgtcacg ccaagggcgg atttgaccgc ggaccggtgc tgacggcgtg gaacattgac
     1981 ggttcaaccg cacgagtagc ggctttattc gatgtccagt aatcgatatg ccgggagatt
     2041 atttcaacaa acggcgacca gacgccatcg gccagttttg gccgagcgat tcataggcgc
     2101 aatcatagcc caaggggctc ctgaaaggca atgccgccaa agagggggat cggatcagcc
     2161 gagcggctga tatccggccg atcggatggc ctcacggacg cgttcgccgc ccccgggacg
     2221 ggtcccatcc agcgtgacat cgaccgatct cgcggcgagg tcgacgcgga ccgccgtcac
     2281 gccatccacc cggctgagcg cccgctcgac ggccctgacg cagtacgcgc acgtcatgcc
     2341 gtcgataatg acgtgcacgc ggtccgcccc gttgaccccg ccggatgccg gagtaccggc
     2401 gggcgcggac gacgcggcgg cgggatgatg gctgtgttcc acgtcgagtt tcacggggtg
     2461 ttcgtgttga tggaggatgt ctccatgttg gtgggagtgt tcgtgctgcg cagggtgcac
     2521 gaccggcgcc gacaccgcgc gaaccgcccg tctccggaag gagtcgtacg ccagcgccgc
     2581 cgcgaacacc gtggccgcga acagcacgat cgtggcgccg gacgatgcgt tggacagaaa
     2641 actcagatac atgccgccga cgccgcagag caccccgaga ccgatcgacg tgaatacgag
     2701 acggtgaaaa ttgtgcgtga gcagacgagc cgtcgccgct ggaatcacga tggcggccac
     2761 aatgagcatc acgccgagca cctgcatcga caccacgatc gtggccgcga gcaccaacgc
     2821 gaacccggcg tcgatccact cggtccgcac gccatagacc ttggccactt cggggtcgaa
     2881 tgtcgtgaaa agaagttgtt tgtaaaacac aaaaatgaga gccgccgaca cgagcgacac
     2941 gacggcgatg acgatcagat cgtccgccgt cactccgaga atgttcccga agagcgccgc
     3001 ttcaaaattc cgggtgaatt tcctgacccg gctgatcaac acgaccccga ccgcaaagct
     3061 ggcggtcgtg acaatgccga tcacggcgtc ggcgccgatc tttttccgct tggccacgga
     3121 gttgatcaac aacgcggaca gaaatcccca gagcccggcg ccgagataga agttccagct
     3181 catgacgtag ctgacgaccg ctccgccgaa cacggaatgc gagaggccgt ggccgatgta
     3241 gctcatattg cgcaatacga tgtagacgcc gatcaaaccg caaatggcgc cggtcatcgc
     3301 cgcggcgagc agcccgttgc ggaaaaactc atactgcaac ggttcaaata gggtctcgat
     3361 cgccatcctc cgcgcgcacg gttcgcgagt cgtagtgccc cccggcattg gggctgtcgg
     3421 caatgagcag caacccgttt tgccgaacca ccaccatctc agagttgtac gttcgactca
     3481 gtatttccga cgtaaagacc tcttgcgggt ccccttgcgc gacaatcgat cgattaaaac
     3541 agatgaccca cggcagatgg cgggccacgc tgttgagatc gtgggtggtc agaatgatcg
     3601 tcacaccctc gcgactcaac tgcgacaaca gatgaaggat atggtgctgg gtcttgagat
     3661 ccacgcccga cgtcggttcg tcaagaatga gcaaccgcgg gcggctgatg agtgcgcgcg
     3721 ccagaaagac gcgctgctgc tctccgccgg acagggcgcg aatctgccgg tgcgcgtatt
     3781 gccggattcc caagtgctcc aacagatcga gcgcaacctt ccggtcctcc cggctgggcc
     3841 acggcagcca ccccatctcc cggtgccgtc ccatcaacac cacctgctca accgtcaccg
     3901 ggaaattcca gttcacggtt tcgatctgcg ggacataccc caccccgcgg gccgtctccc
     3961 catagatccg ctgccccgcc accgagacgt cgccggcggc gacgggaacc aggcccaata
     4021 acactttcaa cagggtggtt ttgccggacc cgctggggcc cacgactccg gggtagagcc
     4081 cttgggg
//
LOCUS       DBNGTT010000021         3339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_515150, whole genome shotgun sequence.
ACCESSION   DBNGTT010000021 DBNGTT010000000
VERSION     DBNGTT010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_515150"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16BC_00430"
     CDS             complement(<1..206)
                     /locus_tag="AC16BC_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3240104.1"
                     /translation="MTQTVDQEPQQVEIPSRLPMLPVRDLVVFPPMVLPLFVGREMSI
                     KAIDEALAGNRLVLLVAQRALDVE"
     gene            complement(362..775)
                     /locus_tag="AC16BC_00435"
     CDS             complement(362..775)
                     /locus_tag="AC16BC_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3240105.1"
                     /translation="MAKHETVVRMTSERVLSTSLLAGNDAPSTPLLDLYEVSGGLVVE
                     VDLPGVVVEDLRIVVSQNNLTIEGSRRTRGDASRGGYLRVERSSAAFHRVVSLPIPIN
                     PHAATARYERGVLTVTFPKIPDRRQNAIEIPVRDG"
     gene            complement(792..1667)
                     /gene="galU"
                     /locus_tag="AC16BC_00440"
     CDS             complement(792..1667)
                     /gene="galU"
                     /locus_tag="AC16BC_00440"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR3240106.1"
                     /translation="MRASPIVKAVIPAAGLGTRFLPATKASPKEMLPLVDKPLIQYVV
                     EEAVAAGIREIVIVTGRGKRAIEDHFDVAFELETTLRGQGKKKQLDEIQEITDLASFY
                     YVRQREAKGLGHAVLQAKGVVGSDTFAVLLGDDIIDHPVPAIQQLITMYREVGASVIG
                     LQPVTREEVSQYGIIQGREVKPGLYQITDLIEKPSPEQAPSTVAVIGRYVLTGDIFEI
                     LERTEPGRNGEVQLTDALRVLARQGRLYGKVLDGRRHDAGDKLGFLKATVEFGLKNPE
                     LGPAFRRYLEALRLK"
     gene            complement(1678..2316)
                     /gene="coaE"
                     /locus_tag="AC16BC_00445"
     CDS             complement(1678..2316)
                     /gene="coaE"
                     /locus_tag="AC16BC_00445"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933803.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR3240107.1"
                     /translation="MIRVGLTGGIACGKSTVSDMFRSLGAYVIDADQLAREALRPGSP
                     GARAVARAFGRDLAPGGAVDRAKLAALVFGHPRRRKQLEAIVHPRVFAAQRALTRDIA
                     ARDPGAVVIFDAALLIETGAHRTMDAVIVVSADQRTQLARLAARDRLSRRDALARIRS
                     QQPLADKRRLADYVIDGRRPRAEVRRAVARIYAELAERAGGGRRVDIFRHPP"
     gene            2442..>3339
                     /gene="mtgA"
                     /locus_tag="AC16BC_00450"
     CDS             2442..>3339
                     /gene="mtgA"
                     /locus_tag="AC16BC_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR3240108.1"
                     /translation="MTRRARRTHPLRRALAWAALAAAAVTVSIGAYVYLTLPDVRPLK
                     TKNPAVTAFMALRDQEYRQREGRAPKRQQTWVPLRRISPNLVDAVLLAEDAGFYQHDG
                     VDYHELWESMKLNWRDRRLRRGASTITQQLAKNLYLSPKRTITRKARELLIARRLERD
                     LGKRRILEIYLNVVEWGRNVYGAEEAARRYFGVPARDLTPAQGATLAGMLINPIKYNP
                     GAPSSRLARRKRIILERLAKYGKISQQEYRLAMGLEPPRIETVRPEPTRPPPTVEPPD
                     DGLGEDEPIAEPPAAAIPPLELS"
BASE COUNT          519 a         1231 c         1066 g          523 t
ORIGIN      
        1 ttctccacgt ccagcgcgcg ctgcgccacc aacaacacga ggcgattgcc cgcgagcgcc
       61 tcgtcgatcg ccttgatcga catctcccgc cccacgaaca gcgggagcac catgggcggg
      121 aagaccacca agtcccgcac cggcagcatg ggcaaccggc tcgggatttc gacctgctgg
      181 ggttcctgat caaccgtttg ggtcacgcgt ggactccctg tttcgttctc gttatcggtc
      241 atgtttcgtc aatttccatc agccgccggc tcacccgccc gcgcgggact tcccgtggtc
      301 cccccttcgg caacgagggc gaggggagat gtcttgtcca tccgatccgg ccccgaggcc
      361 gtcacccgtc gcgcaccggg atctcgatcg cgttctgccg tcgatccggg atcttgggga
      421 acgtcacggt gagcacgccc cgctcgtacc gcgccgtggc cgcgtggggg ttgatgggga
      481 tgggcaggct caccacccgg tggaacgccg cggaactccg ttcgacccgg agatacccgc
      541 cccgcgacgc gtccccccgc gtccgccggc tgccctcgat ggtcagattg ttttgcgaca
      601 ccacgatccg caggtcctcg acgaccacgc cgggcagatc cacctccacc accaacccgc
      661 ccgagacctc gtacaggtcc aggagcggcg tgctcggggc gtcgtttccc gccaggagcg
      721 acgtggacaa cacccgctcg ctcgtcatcc gcaccacggt ttcgtgtttg gccatgtccg
      781 cccccgccga cctacttgag ccgcagcgct tccagatacc ggcggaacgc cggccccagt
      841 tccgggttct tcaagccgaa ttcgaccgtg gctttgagaa atcccagctt gtccccggcg
      901 tcgtgccgcc gcccgtcaag caccttcccg tacagccgcc cctggcgcgc caacacgcgc
      961 aacgcgtccg tgagctggac ttcgccgttg cggcccggtt ccgtgcgttc cagaatctcg
     1021 aagatgtcgc cggtgagcac gtagcggccg atcaccgcga ccgtggacgg cgcctgttcg
     1081 ggcgacggtt tctcgatcag atccgtgatc tgatacaggc cgggcttgac ctcccggccc
     1141 tggatgatcc cgtattggct gacttcctcc cgggtcacgg gctgcaaccc gatcaccgac
     1201 gcccccacct cccggtacat cgtgatcaat tgctggatcg cggggaccgg atggtcgatg
     1261 atgtcgtcgc ccaagagaac cgcgaacgtg tccgagccga ccacgccctt ggcctgcagc
     1321 accgcgtggc cgagcccctt ggcctctcgc tgtcgcacgt agtagaaact cgcgaggtcc
     1381 gtgatttcct ggatctcgtc gagctgtttc ttcttgccct ggccgcgcag ggtggtctcc
     1441 agctcgaacg ccacgtcgaa gtggtcctcg atcgcgcgtt tgccgcggcc cgtcacgatc
     1501 acgatctcgc gaatgcccgc ggccaccgcc tcctccacca cgtactgaat caggggcttg
     1561 tcgaccaggg gcaacatctc tttgggcgag gccttggtgg cggggagaaa cctggtgccg
     1621 aggcccgcgg cggggatcac ggccttcacg atcggagagg cgcgcatcgc ggccattcta
     1681 gggaggatgc cggaagatgt caacgcgccg cccgcccccg gcgcgctcgg ccagctcggc
     1741 gtagatgcgc gccaccgcgc gacggacttc ggcgcgcgga cggcgcccgt cgatcacgta
     1801 atccgccagc cgccgcttgt cggcgagcgg ctgctggctc ctgatgcgcg cgagcgcgtc
     1861 ccgccgcgac aacctatcgc gcgcggccag gcgggcgagc tgggtgcgtt gatccgcgct
     1921 caccacgatc accgcgtcca tcgtgcggtg cgcgccggtc tcgatcaaca acgcggcgtc
     1981 gaagatcacg accgcgcccg ggtcgcgggc cgcgatgtcg cgggtgagcg cgcgctgggc
     2041 cgcgaacacc cgcgggtgca cgatcgcttc cagttgtttg cgccgtcgcg ggtgcccgaa
     2101 caccagcgcg gccagtttgg cgcggtccac cgccccgccg ggcgcaagat cccggccgaa
     2161 tgcccgggcc accgcgcgcg cgccgggcga cccggggcgc agcgcctcgc gggcgagttg
     2221 atccgcgtcg atcacgtagg cgccgagcga tcggaacatg tcgctcaccg tgctctttcc
     2281 gcacgcaatc ccgcccgtca agccgacccg gatcatcgcg gcccgcagtg tagcacggat
     2341 cgaacgcgac gggccgccac gttgatccgg tcggtcccgg ccgcgtacaa tccccggcga
     2401 tcgcgcatga ccgaccgtct ccccaccacc ccgttgttcg cgtgacccgc cgcgcccgac
     2461 gaacccatcc gctccggcgc gcattggcct gggcggcgct ggccgcggcc gccgtgaccg
     2521 tgtcgatcgg cgcgtacgtg tatctcaccc tgcccgacgt gcggccgctc aaaaccaaaa
     2581 accccgcggt caccgcgttc atggccttgc gcgatcagga gtatcggcaa cgcgagggcc
     2641 gtgcgcccaa acggcaacag acctgggtgc cgctccgacg gatctcgccc aacctggtgg
     2701 acgcggtgct gctggccgaa gacgccgggt tctaccagca cgacggggtg gattaccacg
     2761 aactgtggga gtccatgaag ctcaattggc gcgatcgccg cctgcggcgc ggcgccagca
     2821 cgatcaccca gcagctcgcg aagaacctgt acctgtcgcc caagcgcacg atcacgcgca
     2881 aggcgcgtga actgctgatc gccaggcgcc tggaacgaga cctgggcaag cgccgcattt
     2941 tggaaatcta cctcaacgtg gtggagtggg gacgcaacgt gtacggcgcg gaggaagcgg
     3001 cgcggcgcta cttcggggtg ccggcccgcg acctcacccc cgcgcaaggc gccacgctcg
     3061 cgggcatgtt gatcaatccg atcaagtaca acccgggcgc gccgtcctcc cggctcgccc
     3121 ggcgcaagcg catcatcctg gagcgcctgg cgaaatacgg gaagatctcg cagcaggagt
     3181 accgcctggc catgggcctt gagccgccgc ggatcgaaac cgtgcgcccc gagccgaccc
     3241 ggcccccgcc cacggttgaa ccgcccgacg acgggctcgg cgaggatgaa ccgatcgcgg
     3301 aacccccggc cgcggccatc ccgccgctgg agctgagcg
//
LOCUS       DBNGTT010000022         3405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2814826, whole genome shotgun sequence.
ACCESSION   DBNGTT010000022 DBNGTT010000000
VERSION     DBNGTT010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2814826"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="AC16BC_00455"
     CDS             <1..1021
                     /locus_tag="AC16BC_00455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3240109.1"
                     /translation="YEAAVSFQLFRSMFDLFLPKQLGFKVTPKGLRSHRRAFDFNSST
                     LPLIATGITLVAIAKGLWEFFYFGIEKDAYFFNLSWASLNLLFLLMGLLVAWERPQQR
                     TEERIRKSLPFELESAGVSLKGTTHDLSVSGLSFMTAPDETIPLSGEVTLGEGTPIRC
                     RYRVVYHDCVSRQASRCGIAFLDLPHDDRCKLIVDLFADAGTWNQAHATRVRGNLPMA
                     FHLFRGIVRCLRPLQRRTRTSPRSKTLRRIAMRVGSEQRRALLRDWSSRGIGILHIGK
                     EWPSTMGGELSADRFHDATFQEVYCKRLLPRVWRIGLRIATRPVVQTIPAPVQGREMA
                     LHQDH"
     gene            1084..2616
                     /locus_tag="AC16BC_00460"
     CDS             1084..2616
                     /locus_tag="AC16BC_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240110.1"
                     /translation="MPLSAATASAAGFSESWYMFRGNANMEIGNYKAAIEAFEKVIEL
                     NPDNRDAMRSLGLAYERQGLKDKSIEVFDRYLRKYDDDPEIAFKQAEALGWSRYAYRE
                     KDMLKYYQMGLKQKDDPKRRLKYASHLAKTRDTSREAVVQYERVLAVQPRNAEAHRGL
                     AKAYAWLGDRDRALYHANLADRYAMTETGDLTALRRDMSKGREPTLEGIAGALAQPGE
                     PFELYAVRLGSRVKGDLTPFTTTTLEAGLEYFGDSSDNATGGYLAFGTQARLNPSNRI
                     DGMVEYHGAALANPLAYTIEYTYAAASFSLSPGVKREIKYDSFPALVGRRVSGQLIGA
                     ARATLFYTDLAVSKGGLQVDTTPFAGWVEGDSLDNNGQLGLDAKVALPFWSRDAWTVS
                     GEYLLYATHYANDQSEFVTGAEPLAGYFSPRLFVNQTPRLVATHVSPTKNELSVAAGP
                     AIQYIDEANDDPALRVGADAHLSYTAHAPDRLLLKFMADVTQITGIQTRIQLQGLLVY
                     TF"
     gene            2708..2905
                     /locus_tag="AC16BC_00465"
                     /pseudo
     CDS             2708..2905
                     /locus_tag="AC16BC_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492198.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2983..>3405)
                     /locus_tag="AC16BC_00470"
     CDS             complement(2983..>3405)
                     /locus_tag="AC16BC_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3240111.1"
                     /translation="NRREAHQAAGQVALVQAVQTHLDWLAHQIADLERTITDHIDRHP
                     DLKGDAALLRSIPGLGPTTVAKVLAYAGDVRRFANAKALAAFIGVTPRQRVSGSSVKG
                     RTMMSRTGHAALRRALYMPGLVARRHNPVLRGAPRGQT"
BASE COUNT          641 a         1127 c         1040 g          597 t
ORIGIN      
        1 gtacgaagcc gcggtgagct ttcagctctt tcggtccatg ttcgatctct tcctgcccaa
       61 acaactggga ttcaaggtca caccaaaggg cttgcggtcc catcggcggg cgtttgattt
      121 caactcctcg accctccccc tcatcgcgac cgggatcacc ctcgtggcga tcgccaaagg
      181 cctctgggag ttcttctact tcggcattga gaaggacgcc tactttttca acctgagctg
      241 ggcctcgctc aacctgctgt tcctgctcat gggcctgctg gtcgcctggg aacgaccgca
      301 gcaacgaacc gaagagcgga tcaggaagtc gctgccgttc gagctggaga gcgccggcgt
      361 gtcgctgaag ggaaccacgc acgacctcag cgtgtccggg ctctcgttca tgaccgcgcc
      421 ggacgagacc atcccgctgt ccggggaggt caccctcggt gagggcacgc cgatccggtg
      481 ccggtatcgg gtcgtgtacc acgactgcgt ctcgcgccaa gcatcgcgat gcgggatcgc
      541 atttctggac ctgccccacg atgaccggtg caagctgatc gtcgatctgt tcgccgacgc
      601 cggaacctgg aaccaggctc atgccacccg cgtgcgcggc aatttgccga tggccttcca
      661 tctgttccgc ggcatcgtgc gatgcctgcg acccttgcaa cgccgcaccc gaacctcccc
      721 gcggagcaaa accctgcgcc gcatcgcaat gcgggtcggg agtgagcagc ggcgcgcgct
      781 gttgagagac tggtcgtcac gcgggatcgg aatcctgcac atcggcaagg aatggccttc
      841 gaccatgggg ggcgagttgt cggctgaccg cttccacgac gcaacgttcc aggaggtgta
      901 ttgcaaacgc ctgctgcctc gtgtgtggcg catagggctg cgcatcgcca cccgcccggt
      961 cgttcagaca atccccgctc ccgtgcaagg gcgtgagatg gccctgcacc aggaccatta
     1021 ggtcccagag gtctctgctt ggcttggagg aatacatttg ttgcgctgtt gatggttctc
     1081 gccatgcccc tcagcgccgc cactgcgtcc gcggctgggt tcagcgaatc gtggtacatg
     1141 ttccggggca acgccaacat ggagatcggc aactacaagg ccgcgatcga ggcgttcgag
     1201 aaagtcatcg agctcaaccc ggacaatcgg gacgcgatgc gaagccttgg cctggcctat
     1261 gagcgacaag gtttgaagga caagtccatc gaggtgttcg accgctatct ccggaagtac
     1321 gatgacgatc cggagatcgc tttcaaacag gccgaggccc tgggatggtc gcggtacgcc
     1381 taccgcgaga aggacatgct gaagtactac caaatgggct tgaagcaaaa ggacgacccc
     1441 aaacggcgcc tcaagtacgc ctcacatctc gccaaaaccc gggacaccag ccgggaagcg
     1501 gtggttcagt acgagcgggt ccttgcggtg cagccccgca acgcggaggc gcaccgcggc
     1561 cttgcgaaag cctacgcctg gctcggcgac cgcgaccgcg ccctctatca cgccaacctg
     1621 gcggaccgct acgccatgac cgagaccggc gatctgaccg cgctgcggcg cgacatgagc
     1681 aagggccgcg aacccaccct cgaaggcatc gccggagccc tcgcccagcc cggagagccg
     1741 ttcgagctgt acgccgtccg gctgggctcg cgcgtcaagg gcgacctgac gccgttcacc
     1801 accaccacgc tcgaagccgg tctcgagtac ttcggggatt cgtccgacaa tgcgaccggc
     1861 ggctacctcg cgttcggcac ccaggcccgc ctcaatccgt ccaatcgtat cgacggcatg
     1921 gtggagtatc atggggccgc gctggccaat cctctggcct acaccatcga gtacacgtat
     1981 gccgcggcgt cgttctcctt gagccctggc gtgaaacgcg agatcaaata cgattccttc
     2041 cccgccctgg tggggcgtcg agtctccggc caactcatcg gcgccgcgcg cgccaccttg
     2101 ttctacaccg acctggctgt ttccaaaggc gggctccagg tcgacaccac cccgtttgcc
     2161 ggctgggtcg aaggcgattc cttggacaac aatgggcagt tgggactgga cgcgaaagtc
     2221 gccctgccgt tctggagccg cgatgcctgg accgtgtccg gcgagtacct cttgtacgcg
     2281 acccactacg ccaacgacca atcagaattc gtgaccggcg ccgaacccct ggccggctac
     2341 ttcagccccc gcctgttcgt caaccaaacc ccgcgcctgg tggccacgca cgtgtcaccc
     2401 accaagaacg aactctccgt ggccgcaggc ccggcgatcc agtacatcga cgaagcgaac
     2461 gacgaccccg cgctgcgagt cggcgccgac gcgcacctct cctacaccgc gcacgcgcct
     2521 gaccggttgc tgctcaaatt catggcggac gtgacccaga tcaccggtat ccaaacacgc
     2581 atccagttgc aggggctctt ggtgtacacg ttctgatcac caacggtagt ccaggtcacg
     2641 gcgggaggga tgatcgggaa tcttgacatc gcgagcggaa cgggcgcaga gtaacgcccg
     2701 aagccgatcc atcacaggtt ggccatccgg ctggtccatc gattcgcagg acacgctggc
     2761 gttcagcgcc ctgacgggca tgcggtccat gaacgaagtc ttcctcctgg agcgcgcagc
     2821 agaggcctac gatcgaatga tgagcgggaa ggcgcggttt cgagtggtgc taacaaccgg
     2881 gtcgcgggag aaggagaagt cctgagacgt gaacctatcg aatttcagaa cctacccatg
     2941 ctcggctcag ttggtggttg ccaatattga aaagtcaaca agtcaagtct gaccccgcgg
     3001 agccccgcgc aacactggat tatgacgccg ggccactagg ccgggcatgt agagcgcgcg
     3061 gcgtaaggcg gcatgcccgg tccgactcat catggtgcgg cctttgaccg aactcccgga
     3121 cacccgctgc cggggcgtga ccccgatgaa cgcggccagc gccttggcat tggcaaagcg
     3181 gcggacatcg cccgcatagg ccagcacctt cgccaccgtc gtcgggccca ggccgggaat
     3241 actgcgcagt aacgcggcat cgcccttgag atccgggtgc cgatcgatgt ggtcggtgat
     3301 ggtccgctcc aagtcggcga tctggtgcgc taaccagtcc agatgggttt ggacggcttg
     3361 gaccagcgcc acctggcccg ccgcttggtg ggcttcccgc cggtt
//
LOCUS       DBNGTT010000023         2081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2176788, whole genome shotgun sequence.
ACCESSION   DBNGTT010000023 DBNGTT010000000
VERSION     DBNGTT010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2176788"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="AC16BC_00475"
     CDS             complement(<1..560)
                     /locus_tag="AC16BC_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3240112.1"
                     /translation="MGTDQADVDHQAAEYGIIKPWFEDEHPAHSVNLAAYYLDKYEVT
                     NADYQTFVRATNRRPPPDWSGPTHPQGLPRGPVVSVTWDDADAYCRWAGKRLPTEAEW
                     EKAARGFEGRLYPWGNEFELGHANIGGGRNGPVAVGSYPSGRTPLGVYDLVGNVWEWT
                     ADWYQPYPGNPAMSASDPPPGRVLRGS"
     gene            complement(755..1663)
                     /locus_tag="AC16BC_00480"
     CDS             complement(755..1663)
                     /locus_tag="AC16BC_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3240113.1"
                     /translation="MPIAPTTHTVCGRCHTGSGTPVVQPMTDPACAECHLFGSRGATV
                     EPQLARVAAAGASSVPSNAAGRPIKQSLVAIPGGEFVMGNDGRGDDGPGDEDERPAHR
                     VTVNAFRIDRYETTNAMYAEFVRATHREPPFHWVNGRIPDGKADHPVVYVSWHDADAF
                     CRWAGKRLPTEAEWEKAARGTDGRRFPWGDQFSPAKANTPQYWLTQHAGGDTMPVGSF
                     PQGRTPEGVEDMAGNVYEWVADWYQPYPGNRFPNVHYGTKNKVLRGGSWYDCLSYGCG
                     LSSPVYNRSRFAPDIKNKGFGFRCAQ"
BASE COUNT          334 a          682 c          675 g          390 t
ORIGIN      
        1 gagcccctga gcacgcggcc gggcggcgga tcgctcgcgg acatggcggg gttgccggga
       61 tagggttggt accagtccgc ggtccactcc cacacgttgc cgaccagatc gtagacgccg
      121 agcggcgtcc gaccgctcgg ataactgccc accgccacgg gcccgtttct cccgcccccg
      181 atgttcgcgt ggcccagttc gaactcgttg ccccaggggt agagccgtcc ttcgaatccg
      241 cgcgcggcct tttcccattc cgcctcggtg gggagacgtt tgccggccca tcggcagtac
      301 gcgtccgcgt cgtcccaggt gaccgagacc acgggaccgc gcggcaggcc ctgcgggtga
      361 gtcgggccgg accagtccgg cggaggacgt cggttggttg cgcgtacgaa cgtttggtag
      421 tccgcgttgg tgacttcgta cttgtccagg taatacgccg cgaggttcac gctgtgcgcg
      481 gggtgctcgt cctcgaacca cggtttgatg atcccgtatt cagcggcttg atggtcgacg
      541 tccgcttgat ccgtgcccat cacgaacggg ccctccggga tgtggaccat cccgggagga
      601 gttggggtgc aggccgtcag tgcgagaaag atgaggggga ggtgggggga gcgggggagc
      661 cggagaaaaa actgaggtcc tttccgagcg ctttgcccct catggttttg ctcttgcgcc
      721 cgctctccca ctctccccac ctccggttcc atctttactg cgcgcatcga aacccgaatc
      781 ccttgttctt gatgtccggg gcgaaccggc tgcgattata caccggcgaa ctcagcccgc
      841 agccgtacga caggcagtcg taccaggacc cgccgcgcag aaccttgttc ttggtcccgt
      901 agtgtacgtt gggaaaccgg ttgccgggat acggctggta ccagtccgcc acccactcat
      961 agacgttgcc cgccatgtcc tcgacgcctt cgggcgtccg cccctgcggg aacgagccca
     1021 ccggcatggt gtcgccgccc gcgtgttggg tgagccagta ctgcggcgtg ttggcttttg
     1081 cgggcgagaa ctgatcgccc catggaaacc gccgtccatc agtaccgcgc gcggcctttt
     1141 cccattccgc ctcggtgggg aggcgtttgc cggcccagcg gcagaacgcg tccgcgtcgt
     1201 gccaactcac gtacaccacc ggatggtcgg ccttcccatc cgggatccgc ccgttgaccc
     1261 agtgaaaggg cggctcgcgg tgcgtggcgc ggacaaactc cgcgtacatc gcgttggtgg
     1321 tttcgtaccg gtcgatccga aaggcattga ccgtcacgcg gtgcgcgggg cgttcgtcct
     1381 catcgccggg cccgtcgtcg ccccggccgt cattgcccat cacgaactcg ccgccgggga
     1441 tggcgaccag actctgtttg atcgggcgtc cggctgcatt gctcggtacc gacgatgcgc
     1501 cggccgcggc cacgcgcgcc agttgcggtt ccacggtcgc gccacggctt ccgaacaggt
     1561 ggcactccgc gcacgcggga tcggtcatgg gctgcaccac cggtgttccc gacccggtat
     1621 ggcagcggcc gcacacggtg tgcgtggtgg gggcgatagg cattgcggcg gatggcgctt
     1681 ctcccgttga tggcggctcc gcgcccgctg atccggtcat ggccgatccg cagatcaccg
     1741 aggctaacac gacggagagg ccaataataa taggtccctg aagaagtcct gaacgtggtt
     1801 cgacaggctc accacgaacg ggaatcttgg cgaaaaaacc atatctaatc cgttcgtcct
     1861 gagcgtgtcg aaggaccccc acggggtctt taggcgatct gacagggagg cagtctcggc
     1921 tcatcgcggc gtgtacacat atcaaatgtc ggggaaaaag gccagggcgc ggcctcaacg
     1981 tcggcggttg tcattcaacg ctgacacacc agtccaaaga caaatccccc catccccccc
     2041 tttttcaaag gggggcgagc cgttgatcct ggcgttgtcc a
//
LOCUS       DBNGTT010000024         1931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1282585, whole genome shotgun sequence.
ACCESSION   DBNGTT010000024 DBNGTT010000000
VERSION     DBNGTT010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1282585"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..989)
                     /gene="mazG"
                     /locus_tag="AC16BC_00485"
     CDS             complement(162..989)
                     /gene="mazG"
                     /locus_tag="AC16BC_00485"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR3240114.1"
                     /translation="MNPPADPERCGCRRVAEIMAALRAPDGCPWDREQTHESLVPYLI
                     EETYEAVDAIAEGKPGALKEELGDVALQIAFHAQLAAEAGTFTLDEVFRACAEKLVRR
                     HPHVFDAAAARARTADDVVSQWDAIKQREGKPPRGPSLLDGAPKGLPALAKAQHIQTR
                     AARVGFDWVKAEDVLAKLDEEIIELRETLAENDRDRTAHELGDVLFTVVNLARRLNLD
                     PESALRGSIARFASRFAHMERAAARPLAELGAHELDRLWEQAKIAETAGSDSDGPSR"
     gene            complement(986..1732)
                     /locus_tag="AC16BC_00490"
     CDS             complement(986..1732)
                     /locus_tag="AC16BC_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010321828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3240115.1"
                     /translation="MKLKGKVALVTGGGSGIGAAVARAFAAAGAEVAICGRRADALEA
                     TRGRIEPVGGRAAVIPADLTRADAIERLINTISGRFGRLDVLVNNAGVLGAHAPIAEY
                     PDQEWDAVIAANLTAVFRLTKAALPLMSRGGSIINVSSGVGRVGRAGWGAYCVSKFGV
                     EGLTQVLAEELREREIRVNAVNPGGTRTAMRAAAYPYEDPATVPTPDDVAPIFVFLAS
                     NESDGVTGRSFDARGWRPAPELVPTRGPAA"
BASE COUNT          311 a          697 c          604 g          319 t
ORIGIN      
        1 gcggggtcgg caaatgagtg ggcaacaacg agtcccttac gcttcgaagc cttcctgccg
       61 cgtgcggctg gaaggcttca gcgaccagtg taatttcaac aacttcagta taggagatgg
      121 ggtacgcgat tgcaaggcaa ccgggccggc gcccggctcg gtcatcgcga tggaccgtcc
      181 gaatccgacc ccgcggtttc cgcgattttc gcctgttccc acagccgatc caactcgtga
      241 gctccaagct cggccagcgg gcgggcggcc gcgcgttcca tgtgcgcaaa acgcgacgca
      301 aacctggcaa tgctcccccg taacgcggat tccgggtcca aattcagccg ccgggccaaa
      361 ttcacgaccg taaagagcac gtcgcccaat tcgtgcgccg tgcgatcccg gtcattctcc
      421 gcgagcgtct cgcgcaactc gatgatctcc tcatcgagct tggccagcac gtcctcggcc
      481 ttgacccagt cgaaccccac gcgcgcggcg cgcgtttgga tgtgttgggc cttggccaac
      541 gccggcaacc ccttcggcgc gccgtccaac agcgacggcc cacgcggcgg cttgccttcg
      601 cgttgtttga tcgcatccca ctggctcacc acgtcgtccg cggtgcgggc gcgcgccgcg
      661 gccgcatcga acacgtgagg gtgccggcgg accaacttct cggcgcacgc ccggaacacc
      721 tcatccagcg tgaacgttcc ggcttccgcg gcgagttgcg cgtgaaacgc aatctgcagc
      781 gccacgtcgc ccaactcctc cttgagcgcg ccgggcttgc cctcggcaat ggcgtcaacc
      841 gcttcgtagg tttcttcgat caggtacggc acgagcgact cgtgcgtctg ctcgcgatcc
      901 caggggcaac cgtctggcgc gcgcagcgcg gccatgatct cggccacgcg gcgacaaccg
      961 cagcgctccg gatcagccgg cggattcatg ccgcgggtcc ccgcgtgggc acgagttcag
     1021 gcgccggtcg ccagccgcgc gcgtcgaacg accggccggt cacaccgtcg gactcgttgg
     1081 aggccaaaaa cacgaagatc ggcgcgacgt catcgggagt cggtacggtg gccgggtcct
     1141 cgtacggata cgcggccgcg cgcatggcgg tgcgcgtccc gcccggattc acggcgttga
     1201 cgcgaatctc ccgctcgcgc aactcctcgg ccaacacctg ggtcaaaccc tccaccccga
     1261 acttggacac gcaataggcg ccccagccgg cgcgcccgac ccgtccgacc ccggaggaca
     1321 cgttgatgat tgagccgcca cgggacatga gcggcagcgc ggccttggtc aggcggaaca
     1381 ccgcggtcag gttggccgcg atcacggcgt cccattcctg gtccgggtac tccgcgatcg
     1441 gcgcgtgcgc gccgaggacc ccggcgttgt tgaccaatac gtcgagacga ccgaaccgac
     1501 cgctgatggt gttgatgagc cgttcaatgg catcggctcg tgtgagatcg gccggtatga
     1561 ccgcggcgcg cccgccgacg ggttcgatcc gcccgcgcgt cgcctccaac gcgtccgccc
     1621 gccgcccgca gatcgcgact tcggcgcctg cggccgcaaa cgcccgcgcc accgccgcgc
     1681 cgatcccgct gccgccgccc gtcactagtg cgacctttcc cttcagcttc acgaggtcct
     1741 tctaacacgc ggttttgcgg caagtcaaac ccggggcgtg tgccccacgc gccccggttg
     1801 attcagcgcg ggcatccggt tatggtaccg gcgcgcggaa attgatcctc tttatcgcca
     1861 ccagtcttga cggctacatt gcgggcccgg cctgagagat cgactggttg tccaccgacc
     1921 aggactacgg g
//
LOCUS       DBNGTT010000025         8794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1921647, whole genome shotgun sequence.
ACCESSION   DBNGTT010000025 DBNGTT010000000
VERSION     DBNGTT010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1921647"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="AC16BC_00495"
     CDS             complement(<1..541)
                     /locus_tag="AC16BC_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HIR3240116.1"
                     /translation="MKPKRRPGEVRDAIFAVLSERPEGATTSQIERGVQHLLGRSAPS
                     SVRSYLRLNTDSLFVRSTRGHYSLLENVPPAHSVDPPNDVRDFPSFSVGNTRVYNADC
                     LEWLRTHPQNSVHAVVTDPPYGLFEYTPEQQEKLRNGHGGVWRIPPSFDGAKRSPLPR
                     FTVLSDNDLRNLERFFFEWG"
     gene            1121..1306
                     /locus_tag="AC16BC_00500"
     CDS             1121..1306
                     /locus_tag="AC16BC_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240117.1"
                     /translation="MVAANRGLFPEIIHSTYSPRFGIGWECDAPEEAFMVIELTPGYL
                     RGAPGKVRYHTAFSDVG"
     gene            complement(1446..2672)
                     /locus_tag="AC16BC_00505"
     CDS             complement(1446..2672)
                     /locus_tag="AC16BC_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3240118.1"
                     /translation="MGGLTVGVAGACLYWSDIEHFFYVILGLQAAILLLYGAQRIAPS
                     ISRERADRTWDFQRLTPQSSWDIVLGKLLGAPLYAYFLAAAFIPWLIVAQASDTSVPL
                     DVACRSVGLLATITFGVLAIALMGSAYTERDKTAQAATVGALLSVMGLFTMGGLTFRS
                     APGNAAFYGFHVSPDALVMFSAVAAGLWALAAAAWRVGLDLLEPRRAWRLPAFMAFFA
                     WYIVGLMRDTPGYEEGAAAMTAVAASLAVLYVAAWSNREGIDDWKRWAAATGSNRLDR
                     APLWVIGLVTAAGIAAVVAALLPLGSAGRIVLLLPLFAARDLLFLQWCRLTKSRRPDA
                     MATIYLTLVYALPLIVLGSLRVNQAIFVFVPFPDERLGAAINAAPVAAQTALMAWAVA
                     RSLAPHLAGAQQSPAA"
     gene            complement(2744..3682)
                     /locus_tag="AC16BC_00510"
     CDS             complement(2744..3682)
                     /locus_tag="AC16BC_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240119.1"
                     /translation="MTADWIIETRALRREFGDTVAVSDVTLRVPKGVVLALVGPNGAG
                     KTTLLKMLSGLLEPTSGTAVVGQIDVRERPRDVHALLGFLPDFFGLYDDLMVEDYLDV
                     FARAYRVRPEQRAGRVRDALDRVGLSERGATRVGTLSRGMRQRLGIARTLIHDPPLLL
                     LDEPASGLDPEIRYELQELFVNLARSGKTLVVSSHILAELEAYCTHVAVLRGGRLVAS
                     GSIEDVRRGLSARRRIRIRAADRWDAISQILGARPTVSGISQSGGDGVFEHAGEESDL
                     VDILRALVSAGVPVSAFAEDRGTIQDTYLALMEERS"
     gene            complement(3879..4100)
                     /locus_tag="AC16BC_00515"
     CDS             complement(3879..4100)
                     /locus_tag="AC16BC_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240120.1"
                     /translation="MKVIGQQTPGKTSGLSLGKEMPQTPHEIRPVAVVAEDLALLIAS
                     HHHMMQHPRCVESRPTRHGEAYSTIRAIR"
     gene            4193..4912
                     /locus_tag="AC16BC_00520"
     CDS             4193..4912
                     /locus_tag="AC16BC_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240121.1"
                     /translation="MGHLFQNRYRSIVCEEEPYLLALVRYIHLNPVRAGVVSGLKTLA
                     TYPWCGHGALLGRDQHPWQATAEVLARFGKTVEQARREYQTFMAAGIDSGEGRPSSSS
                     ENWNATNRSSYERVSAALPGAIARGVLGSPEFAETVRAGSDRRRGSSEADCRAVVLAV
                     CRTLGLSPEALASGRRTRPLSNGRAVVAFVARTKYRVASSELAAALGTSPQAVLLAER
                     RGAQLLKRRTDLKSSLKELRI"
     gene            4983..5312
                     /locus_tag="AC16BC_00525"
     CDS             4983..5312
                     /locus_tag="AC16BC_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3240122.1"
                     /translation="MDIAGPGAKLVRYDGAERFDVLPLLVATDGAIAAFGHDPRRLRP
                     NLVIGGVEGMAEREWPGGCLRIGTALIGVQDLRSRCIMTSYDPDTQVREKPSPAASIS
                     ASRASSH"
     gene            5504..5905
                     /locus_tag="AC16BC_00530"
     CDS             5504..5905
                     /locus_tag="AC16BC_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3240123.1"
                     /translation="MFLWFITAVFGVRAGLLVWWGQWGVMAIHVAGMAVFVGLAVFFR
                     RTTRYTLTQEAVYRSAGMFGRTDQRLPLSEFESVDVGRSRLDQLLGIGTVLLCREDGT
                     SERLAGLRDPEVIQRKIEALLRPTKLTGKTN"
     gene            6050..6754
                     /locus_tag="AC16BC_00535"
     CDS             6050..6754
                     /locus_tag="AC16BC_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HIR3240124.1"
                     /translation="MSKTVNHRQSCAVCGKTFAARDVVAATFVRDAVADEIRRDHPGW
                     APESFICRPDLTRYRTKYVHAILASEKGEVTSLEQEVLHSLREHDILATNVDMEFERE
                     WSFGERMADRIATFGGSWMFLICFGLFVTLWIVMNSLVLLWRPADPYPYILLNLVLSC
                     LAAIQAPIIMMSQNRQEAKDRVRSQHDYQVNLKAELEIRHLHEKIDHLLSHQWERLVQ
                     IQELQLELLAELDRRR"
     gene            6870..7352
                     /locus_tag="AC16BC_00540"
     CDS             6870..7352
                     /locus_tag="AC16BC_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_function: GO:0072570 - ADP-D-ribose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR3240125.1"
                     /translation="MITEVTGDILLSGAAVAHGIAPNDNFAGGLALALRTHWPGMYKD
                     FRHYCQVSSPKPGEVWVWSGPGVRIVSLFTQDPPPAHGDSPGKARLESVNHCLRALRK
                     AVDSEKLTSLALPRLATGVGGLDWKEVKPLIDKHLGDALIPVFLYSTYQPHVKAREVG
                     "
     gene            7405..8175
                     /locus_tag="AC16BC_00545"
     CDS             7405..8175
                     /locus_tag="AC16BC_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HIR3240126.1"
                     /translation="MLEPLGRALARYLTKPRDRGAQVSTSRPGALAAAIRKGDVLLVE
                     GNSRVSVAIKYLTQSSWSHAALCIGEALGAPPPGQDRRVLVDADMLDGVRAIPLSAYA
                     AFNTRICRPVGLSPAEIETVAAFAVSRLGHQYDLKNVFDLTRYVLRTPPVPSRWRRRL
                     LRLGSGDPTRAICSTLIAEAFESVRYPILPEVVFERSDDPACADCYREVLHIRHHSLF
                     VPRDFDVSPYFQIVKPTIEQGFDPHALTWNQTEDAGLS"
     gene            complement(8233..>8794)
                     /locus_tag="AC16BC_00550"
     CDS             complement(8233..>8794)
                     /locus_tag="AC16BC_00550"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR3240127.1"
                     /translation="VSLSPLEKRGEGFLLWHMERANESQAWLYLPELRQVRHLAISGH
                     EDHAHSHDPNEDLNLGFEQLGTRLTGLNGALIGKETLDGVTHVIIEDRSTATADLLPL
                     RRFWISPSDWTIGKIEYRNADGRLARTQRITWTRIGGARVWTRTEIQPAESPDKTVVE
                     LRDIVVNTDLADRLFTVETLKSGRVP"
BASE COUNT         1594 a         2650 c         2951 g         1599 t
ORIGIN      
        1 ggccccattc gaaaaagaac cgctccagat tccgaaggtc gttgtcggac agaacggtaa
       61 atcgtggtaa cggagatcgc tttgcgccat caaatgaggg gggaatgcgc catactcccc
      121 cgtggccgtt tctgagtttt tcctgctgtt ccggcgtgta ttcgaagaga ccgtaaggag
      181 gatcggtgac tactgcgtgc accgagtttt gggggtgtgt gcgcagccac tccaaacaat
      241 ctgcgttata gacccgagta ttgccgacgg agaacgaagg gaagtcccgg acgtcgttag
      301 gcggatcaac cgaatgggcg ggcgggacgt tttccaggag cgagtagtga cctcgtgtgc
      361 tgcggacgaa gagcgagtcg gtattaagtc gtagataaga ccggacgctg gagggggcag
      421 agcggcccag caagtgctgg acaccgcgtt caatctggga agtagttgct ccctcgggtc
      481 gctcagaaag tactgcgaag attgcatccc tgacttcccc agggcgtcgt ttcggcttca
      541 tctcatcctc cgtgatgagt tggtggcgga catatatatg acgtctagac gtcttttgtc
      601 aatccagacg ggattgggga cggtcccaac taaacaagta aagttagcgc tcgccttaac
      661 gtgatcgccc ggatcgggct atagccttcg ccatgccgcg tgggcgcgac ttgacgcacc
      721 ggggtggcaa ggatgtctga ttttcctcgg agcgcggacg cgttcgactt tgaggcgttg
      781 tcggcacaga actaggagaa gtccggcgag atatggaatc aacagacgga gtatgaatac
      841 ttcgtcaccg ttgtgaagtc gagcgatgac gaggtgtttc gtgcggcaac caacgcgttg
      901 acccaatcag ggtattcgat tgcgtatgcg gataaagcgc accaaacagt cgtgggagaa
      961 aaaggcctcc ggctgaacga gtggaattcc atcaccgcta tttactacaa ggcgaccgga
     1021 gagtcatttc aggtgttttt caaaaacgcg atcacgcagg acatcactgg aggatggagg
     1081 agcaatcggg ccaagaaggt ggccacggct ttttgcggcc ttggtcgctg cgaaccgcgg
     1141 cctgtttcct gaaattattc actccacgta ctccccgcgg ttcgggatcg gttgggagtg
     1201 cgacgcgccc gaggaggcgt tcatggtgat cgagctgacg cccgggtacc tgcgcggcgc
     1261 gcccggtaag gtcagatacc atacggcgtt cagcgacgtg gggtgagacg acgaaggtgt
     1321 cgggaaccat ggcttctccc gggattgcgg agcgtgtcag ggccggttcg tctgtataac
     1381 aggaatattg tggtcggagg acgtttccct cattgttgat cccgcacagc gtggttcgag
     1441 gcttctcacg cggcgggtga ttgctgcgct ccggcaaggt ggggcgccag cgagcgtgcg
     1501 acggcccaag ccatgagggc ggtttgtgcg gcgacggggg cggcgttgat cgccgcgccg
     1561 agacgctcgt cgggaaacgg cacgaacacg aagattgcct gattgacgcg gagactaccc
     1621 aaaacgatga gcgggagcgc gtaaaccagg gtgaggtaga tcgtggccat ggcgtcgggc
     1681 cgtcggctct tggtgagacg gcaccactgt aaaaagagca agtcgcgggc cgcgaatagt
     1741 ggaaggagca gcacgatccg tcccgcgctt ccgaggggca gaagggcggc caccaccgcg
     1801 gcgatgcctg ccgccgtgac caggccgatg acccataacg gcgcacggtc gaggcggttc
     1861 gaccccgtgg ccgccgccca ccgcttccaa tcatcaatgc cctcgcgatt gctccaggcg
     1921 gccacgtaaa gtacggccag tgaggcggca accgcggtca tggccgccgc cccttcctca
     1981 tagcccggtg tgtctcgcat aagcccgacg atgtaccatg cgaaaaaggc catgaacgcg
     2041 ggcaagcgcc acgcgcggcg cggttcgagt aagtcgaggc ccacgcgcca cgccgcggct
     2101 gcgagcgccc acaggccggc cgccacggcg ctgaacataa ccaacgcgtc gggggagacg
     2161 tggaacccgt agaacgccgc gttgccgggt gcagaccgga acgtgagtcc ccccatggtg
     2221 aacaacccca tgacgctcaa gagcgctccc accgtggcag cctgcgccgt cttgtcgcgc
     2281 tcggtgtacg cggaacccat gagcgcgatc gccaacacgc cgaaggtgat cgtcgcgagg
     2341 agcccgactg aacggcatgc gacgtccagg gggacggagg tgtcggatgc ttgggcgacg
     2401 atcagccacg gaatgaacgc ggcggcgagg aagtacgcgt ataacggcgc cccgagcagt
     2461 ttgccgagca cgatgtccca ggacgactgc ggcgtcaaac gctgaaaatc ccacgtccga
     2521 tccgcgcgtt cgcgcgaaat gcttggcgca atccgttgcg cgccgtatag cagcaggatg
     2581 gcggcctgaa gtccgaggat cacgtagaag aagtgctcga tgtccgacca atacagacac
     2641 gcgcccgcca cgccgacagt cagcccgccc atgatcagtc gcgtccgtgt ggcctcgctt
     2701 cgcgaataac ggacggccag cggaagctgt gtgatcgccg agatcacgac cgttcctcca
     2761 ttagtgccag atacgtgtct tggatcgttc cccggtcctc cgcgaacgcc gacaccggca
     2821 cgcccgcgct gaccagtgcc cggaggatgt ccacgagatc ggattcctcg cccgcgtgct
     2881 cgaacactcc gtcgccgccg ctttggctga tgccgctcac ggttggtctg gcgccaagga
     2941 tttgtgaaat ggcgtcccat cggtccgcgg cccgaatacg aattcgtcgg cgcgcggaga
     3001 gcccgcgccg aacgtcctcg atcgagcccg atgcgaccaa gcggccgccg cggagtacgg
     3061 cgacgtgcgt gcagtacgct tcaagttcgg ccaggatgtg agaggagacc acgagcgtct
     3121 tgccggagcg agcgaggttg acgaagagct cctggagctc gtagcgaatc tcgggatcga
     3181 gtcccgacgc gggttcgtcc agcagcagca acggcgggtc gtgaatcagg gttcgcgcga
     3241 tgccgaggcg ctggcgcatg ccgcgggaga gcgtgccgac ccgcgtggcg ccacgctccg
     3301 agagcccgac gcgatcgagg gcgtctcgca cccgcccggc acgctgctcg ggcctgaccc
     3361 gatacgcgcg ggcgaacacg tcgagatagt cttcgaccat caggtcgtcg tacaggccga
     3421 agaagtcggg gaggaagccc aggagcgcgt gcacgtcgcg ggggcgttct cgtacgtcaa
     3481 tctggcccac caccgcggtc ccgctcgtgg gttcgagcag gccggacaac atcttgagga
     3541 gcgtggtctt ccccgcgccg ttggggccca ccagtgccag caccacgccc ttcggaaccc
     3601 gcaaggtcac gtccgagacc gccacggtgt cgccaaactc acggcgcaac gcccgggttt
     3661 cgatgatcca gtcggctgtc atccggcctc ctcgtgatcg acgcgcggca atgtatcccg
     3721 cgggcggctt gagactacat gcggccagcc ggcgtgtcaa cgaccaccgc acatatgatc
     3781 ggagaagttt ctgaatggtt acgtcgagaa ccggataggg gacgtcttcg tccgagggga
     3841 cggtcccaat taaacaagta aaaaataagt tctcgccttt acctgatcgc ccggatcgtg
     3901 ctataggctt ccccatgccg cgtgggccgc gactcgacgc accgggggtg ctgcatcatg
     3961 tgatggtgcg aggcaatgag cagcgcgaga tcttccgcca cgacggcgac cgggcggatt
     4021 tcgtgcggcg tctggggcat ctccttcccg agactcagac cgcttgtttt gcctggtgtt
     4081 tgttgtccaa tcaccttcat atggtcgtgc ggtccggccc gcaagggctt gcgccagcca
     4141 tgcgtcggct gctgacggga tacgccgtga cgtttaaccg cggtacggtc gggtgggaca
     4201 cctgtttcag aaccggtatc gctccatcgt gtgtgaggag gagccgtacc tgctggcgtt
     4261 ggtgcgctat atccatctga atccggttcg agctggcgtg gtgagcgggc tgaagacgct
     4321 ggcgacctat ccctggtgtg ggcacggagc cctcctcggt cgcgatcagc acccctggca
     4381 agcgaccgcg gaagtcctgg ctcgattcgg caaaacggtt gagcaggccc gccgggaata
     4441 ccaaacgttt atggccgctg gcatcgattc cggcgagggc cgtccgtcga gcagttccga
     4501 gaactggaat gccaccaacc gttcgagtta cgaacgtgtc tcggctgcgc tacctggggc
     4561 gatcgcccgt ggcgtgttgg gaagcccaga gtttgccgag acggtgcggg caggaagtga
     4621 ccggaggcgg ggttcgtccg aggcggactg ccgcgcggtg gtgctggccg tctgccgcac
     4681 tctgggctta tccccggagg ccttggcgtc agggcgtcgc acccggccac tgtcaaatgg
     4741 tcgggcggtg gtggcatttg tggcgcgaac gaaataccgg gtcgcctcct ccgagctggc
     4801 ggcggccttg ggcaccagcc cgcaggccgt gctgcttgca gaacgccgcg gcgcgcagct
     4861 tctgaagcga cggacggatt taaagagcag cctcaaagaa ttacgtattt aattgggacc
     4921 gtccccacaa ttttctggtg gatggccggc cttggaacag ccagaaggtt gctgccgagg
     4981 ttgtggatat cgccgggccg ggtgcgaagc tggtgcggta tgacggggcc gagcgctttg
     5041 acgtgctgcc gttgttggtg gccactgacg gcgccattgc agcctttggt cacgatcctc
     5101 gacgcctgcg gccgaatctt gtgatcggcg gcgtggaggg catggctgag cgcgagtggc
     5161 cgggcgggtg tcttcgcatc ggcacggcgc tcatcggggt tcaggatctg cgctcgcgct
     5221 gcatcatgac gtcgtacgat ccggacacgc aggtccggga aaagccatca cccgcggcat
     5281 ctataagcgc ttcgagggca agctcgcact gaattgtttt gtgatccaag gcggcgagat
     5341 cgcggtgggc gacgaagtcc acctggtgcg cggtcgagcg tgcgcagagg cggcagcatt
     5401 agcggagtaa tccgggaata gatgtctagc cgtggaccat gactcaccgc gatacgggcg
     5461 cgaggtcccc cgctggagcg gccggccggc gtggagccag tatgtgtttc tctggttcat
     5521 caccgcggtc ttcggcgttc gggccggcct gttagtgtgg tggggccagt ggggtgtcat
     5581 ggcgattcac gtggcgggca tggccgtgtt cgtgggcctc gcggttttct tccgccgcac
     5641 cacccggtac accctgaccc aagaggccgt ctatcgttca gccgggatgt tcggtcgaac
     5701 ggaccaacgg ctgccgttgt ccgagtttga gtcggtcgac gtgggccgga gccggttgga
     5761 tcaactgctc ggcatcggca cggtgttgct gtgccgcgag gatgggacct ccgagcggct
     5821 cgcggggctg agagatcccg aggtgattca acgcaaaatc gaggcgctac tgagacccac
     5881 gaagctgacc ggaaagacca actgaggcca tgacgcgctt cgcggatttt ctgaccacgg
     5941 tgagtcagat gtgcccagcc gcccgcttta ctcgctctcc tcggccgtgc tatagcctcg
     6001 gggcgggcgg cgtggtccgc tggaagttct tctggacgag gggctggcca tgagcaagac
     6061 cgtcaatcat cggcaatcgt gcgctgtgtg cggtaaaacc tttgcggccc gcgacgtggt
     6121 cgcggccacg ttcgtgcgcg acgcggtggc ggacgagatt cggcgcgacc accccggatg
     6181 ggctcccgag agctttatct gtcgaccgga cctgacccgc taccgcacca agtacgtgca
     6241 cgccatcctg gcgtccgaga agggcgaagt gacctcgctg gagcaggagg tgctccacag
     6301 cctgcgcgaa cacgacattc tcgcgacgaa cgtggatatg gagttcgagc gcgagtggtc
     6361 gttcggcgag cggatggcgg accgcatcgc gacgttcggc ggaagttgga tgttcctgat
     6421 ttgcttcggc ctgttcgtga cgctctggat cgtgatgaac tcgctcgtgc tgctgtggcg
     6481 accggcggat ccgtatccct acattctctt gaacctggtg ctctcgtgtt tggccgcgat
     6541 ccaggcgccg atcatcatga tgagccagaa ccgccaggag gccaaggacc gggtgcgctc
     6601 gcaacatgac taccaagtca acttgaaggc cgaattggag attcgacacc tgcacgagaa
     6661 gatcgaccac ctgttgtcgc accagtggga gcgtttggtc cagattcagg aactccaact
     6721 cgaactattg gccgagctcg accggcggcg ataacggcct ccggcgaatc ttcacactcc
     6781 aagacgggcg aacccctagg tcgacgcgct gtgctagaat gccgcggccg cgacagcgcg
     6841 gcaggacgcg ttgaaccagg ggatgacaca tgatcacgga agtcacggga gatatcttgc
     6901 tcagcggcgc ggcggtggcc cacgggatcg cgccgaacga caactttgcc ggcggcttgg
     6961 cgctcgcgtt gcgcacgcac tggcccggca tgtacaagga cttccggcac tactgccagg
     7021 tctcgtctcc caagcccgga gaggtctggg tctggtccgg acccggggtc cgcatcgtca
     7081 gtctcttcac gcaggacccg ccgccagcgc acggcgacag ccccggcaag gcccgcctgg
     7141 aatccgtcaa ccattgcctc cgggcgctac gcaaggccgt cgacagcgag aaactcacga
     7201 gcttggcgct tccccgtctg gccacgggcg tgggtggtct ggactggaaa gaggtcaagc
     7261 cgctgatcga caagcacctg ggtgacgcgc taatcccggt cttcctctat tcgacctacc
     7321 aaccccatgt gaaggcccgg gaggtggggt gagcgcgacg tagccgaccg atgcggccga
     7381 tccgaccgac accgcggtgc ccgcatgctt gagcctctcg gtcgcgcgct ggcgcgctat
     7441 ctgaccaaac cccgcgaccg cggggcccag gtctccacca gtcggcccgg ggcgctcgcc
     7501 gcggcgattc gcaaaggcga cgtgctcctg gtcgagggca acagccgggt cagcgtggcg
     7561 atcaagtacc tcacgcagtc cagttggtcc cacgccgcgt tgtgtatcgg cgaagcgctc
     7621 ggcgcccctc cgccgggtca ggaccgcagg gtgctggtgg acgcggacat gctcgatggc
     7681 gtgcgcgcga tcccgctgag cgcgtatgcc gccttcaaca cccgaatctg ccggccggtg
     7741 ggactctcgc ccgcggagat cgagacggtg gcggcgtttg cggtgtcgcg gttgggacac
     7801 cagtatgacc tgaagaacgt ttttgatctg acgcggtacg tgctgcgcac cccgccggtg
     7861 ccgagccgct ggcgtcgacg cctgcttcga ctgggcagcg gcgatcccac gcgggcgatc
     7921 tgctcgacct tgatcgcgga ggcgttcgag tcggtgcggt atccgatttt gcccgaggtg
     7981 gtgttcgagc ggtcggacga tcccgcgtgc gcggactgtt atcgcgaggt gttgcacatc
     8041 cggcaccaca gcctgttcgt cccgcgcgac ttcgacgtgt ccccgtactt tcagatcgtc
     8101 aaacccacca ttgaacaagg ctttgatccg cacgccttga cctggaacca gaccgaggac
     8161 gcgggcctgt cctgatccat tgcggggggg gggccgcgtc gggaacgcaa gcacccgaag
     8221 gacggcttca ggtcacggga cgcgaccaga cttgagggtt tcgaccgtga agagacggtc
     8281 ggcgagatcc gtgttcacga cgatgtcgcg cagctcgacc acggtcttgt ccggactctc
     8341 cgcgggctgg atctcggtcc gcgtccacac ccgcgccccg ccgatgcgcg tccaggtgat
     8401 gcgctgggtt cgcgccaggc ggccgtcggc gttgcgatac tcgatcttgc cgatcgtcca
     8461 atccgaggga gagatccaga aacgacgcag gggcaagagg tcggcggtgg ccgtggaacg
     8521 gtcctcgatg atcacgtgcg tgacgccgtc gagggtttct tttccgatca gggctccgtt
     8581 gagcccggtc aagcgggttc cgagctgttc gaagccgaga ttcaaatcct cgttgggatc
     8641 atgactgtgc gcgtggtcct cgtgtccgga gatggcgagg tgtcgcactt ggcgcagttc
     8701 gggcaagtac agccaggcct gggactcgtt ggcgcgctcc atgtgccaga gcaaaaagcc
     8761 ctccccgcgt ttttccagcg gcgacagcga gacc
//
LOCUS       DBNGTT010000026         2996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3071386, whole genome shotgun sequence.
ACCESSION   DBNGTT010000026 DBNGTT010000000
VERSION     DBNGTT010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3071386"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="AC16BC_00555"
     CDS             <1..1934
                     /locus_tag="AC16BC_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3240128.1"
                     /translation="GEPIRSGPVADIHSTEPIGYGVNGSFRELVVMPHDTVPTTAQVV
                     TEGNPPGQSIQVAIDAGQTLSFQMPYDLDLAAVKMLNGGTHTTGGGFNFRAESVARRL
                     KNNPDPKLIFSSKTHQDPSTPMMRAYLGDTMVFRLLHTLMNESHTWHLAGHAFRTERY
                     AEKSDLKNSWHVGIAERYDLVTRAGGAQQIAGDYLHYNGRASHLSEGSWGIVRVLDKE
                     ASDLQKLPGRDEIPASAKTVCPSDAPVKTFGVIAADRALKYNPSTPDVIEVDFDRKLQ
                     VSNPSGKIFMLEGEKSKVATGGTQPMPLTLHVNVGDCVKINLKNEMKAGNVSFSADML
                     AFDPKDSHGVNVGNNPGDQTVAPGKTRTYTFYAHPQYGENAALVWDWGNFINGVRDGL
                     FGAIIVGPKGSKYRDPVTGEDVSLKNSWQADVIIDRAIPENAMRSDYRDASLFFQDED
                     NIIGTSFMPYIQQIAGLTGVNYRIEPWSYREEQGCELGLMFTPCVAAESEPATPTIMA
                     HAGDPVRIHVFGAFNEQNQVFSLEGHQWPLKPNMEGADMMSSLQFGGSENLDVYLKEG
                     AGGPYHIPGTYVWQNHRMPYTQAGQWGYLRVLPAADRKIQPLNGGGTVGGKTAEVPAI
                     EEPRAETESAPGPVSMLQR"
     gene            2227..>2996
                     /locus_tag="AC16BC_00560"
     CDS             2227..>2996
                     /locus_tag="AC16BC_00560"
                     /inference="COORDINATES: protein motif:HMM:NF026037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240129.1"
                     /translation="MSRNALWGAVAVAMAAGLMASGTAVAYEEVAVTNGGTITGKVVL
                     TGRVPEPRVFPLVAYPFGPFCKKISDGKGHIVLQEFIVGSGGGLSDAVVALERVEKGK
                     PFTKISSDFVVVDCMFHPADVPDGEQFAVNESGKLRHEHPLVSVIRNPQPITVINKDP
                     IIHNSQVFQSERGNIVLNFPIPVSTEPRGGLIHLQPGKRIAQMICGMHEFMQSWSFAV
                     DNPYYAKTRKNGEFTIDRVPPGTYRMVAWHPHLAVIQR"
BASE COUNT          600 a          869 c         1012 g          515 t
ORIGIN      
        1 cgggtgaacc gattcgcagc ggaccggtgg cggatattca ctccaccgag ccgatcgggt
       61 acggcgtcaa cggcagtttc cgggaattgg tggtgatgcc gcacgacacc gtgcccacca
      121 ccgcgcaggt cgtgaccgag ggcaatccgc ccggtcagag cattcaggtg gcgattgacg
      181 cgggccagac cctgtcgttc cagatgccct acgatttgga tctggccgcg gtcaagatgt
      241 tgaacggcgg cacccacacc acgggcggcg gtttcaactt ccgcgccgaa tccgtggccc
      301 gtcgtttgaa gaacaacccg gatccgaagc tgatcttctc cagcaagacc caccaggacc
      361 cgagcacccc gatgatgcgg gcgtacctcg gcgacaccat ggtgttccgt ctgttgcaca
      421 cgctgatgaa tgaaagccat acctggcatt tggccgggca cgcgttccgg accgaacggt
      481 acgcggaaaa gtccgacctg aagaactcct ggcacgtcgg gatcgcggag cggtacgact
      541 tggtgaccag ggcgggcggc gcccaacaga tcgcgggcga ctacctgcac tacaacggtc
      601 gggcgtctca cctctcggaa gggagctggg gcatcgtccg ggtgctggac aaggaagcgt
      661 ccgacctgca gaagctgccc ggccgcgacg aaattccggc ctcggccaag acggtgtgtc
      721 cgtccgacgc gccggtcaag accttcggcg tgatcgcggc ggatcgggcg ttgaagtaca
      781 acccgagcac cccggacgtg atcgaggtgg atttcgaccg gaaactgcaa gtgagcaacc
      841 cctcgggcaa gattttcatg ctcgagggtg agaagtccaa agtggcgacc ggcgggaccc
      901 agccgatgcc gttgaccctg cacgtgaacg tgggcgactg cgtcaagatc aacttgaaga
      961 acgagatgaa ggccggcaat gtctcgttca gcgcggacat gctggcgttc gatcccaagg
     1021 attcgcacgg ggtgaacgtg ggcaacaacc cgggcgatca gaccgtggcg cccggcaaga
     1081 cccggaccta caccttctac gcgcatccgc aatacggcga aaacgcggcg ttggtgtggg
     1141 attgggggaa tttcatcaac ggcgtgaggg acgggctctt tggcgcgatc atcgtggggc
     1201 cgaagggttc gaagtatcgc gaccccgtga cgggcgaaga cgtgtcgctc aagaactcct
     1261 ggcaggccga cgtgatcatc gatcgggcga ttccggagaa cgccatgcgg tcggactacc
     1321 gcgacgcatc cttgttcttc caggatgagg acaacatcat cgggacctcg ttcatgccct
     1381 acattcagca gatcgcgggc ttgaccgggg tgaattatcg gatcgagccg tggagctacc
     1441 gcgaggagca aggctgcgaa ctggggttga tgttcacgcc ctgcgtcgcg gccgagtccg
     1501 agccggccac gccgacgatc atggcccatg ccggcgatcc ggtgagaatc cacgtgttcg
     1561 gcgcgttcaa cgaacagaac caggtgttca gcctcgaggg gcatcagtgg ccgctcaagc
     1621 cgaacatgga aggcgcggac atgatgagct cgctgcagtt cggcgggtcc gagaacctcg
     1681 acgtgtacct gaaggagggg gccggcggtc cgtaccatat tccggggacc tacgtgtggc
     1741 agaatcaccg gatgccgtat acgcaggccg gtcagtgggg atacctccgg gttctcccgg
     1801 ccgctgatcg gaagattcag ccgctcaacg gcggaggtac ggtgggcggg aagacggctg
     1861 aagtcccggc catcgaagag ccgcgcgcgg agactgaatc ggctccaggt ccggtttcga
     1921 tgttgcagcg gtagcaacga ggcatcatac ggtcacgcat cgggacaggg acgggcccat
     1981 ggcccggcct gtcccgatgc tgtttgggca ggtagagcgg acacgtctcg gcggtgatcg
     2041 gtcgcgggga taaaccgtcg gaggctgaga atggggcatc cgctgtgtta gcatgcgcaa
     2101 ggcatcgaca ggcctccggg cggggcgggg ggaggcaccg cagcggatag ctcaccgagg
     2161 gcccgatcga tggaggacgc ccggctggtc cggggcaggc ggggtttctt ggagaaaggc
     2221 gcactgatga gtcggaacgc actgtggggg gcggttgccg tggcgatggc cgccggtttg
     2281 atggcgagcg gaacggcggt cgcgtacgaa gaagtggccg tcacgaacgg cgggaccatt
     2341 acggggaaag tcgtgttgac cggacgcgtt ccggaaccgc gtgttttccc gttggtggcg
     2401 tatccattcg gtccgttctg caaaaaaatt tccgacggca aggggcacat cgtgctgcag
     2461 gagttcatcg tcggatccgg cggcggtctg tcggacgcgg tcgttgcgct cgagcgcgtt
     2521 gagaagggca agcccttcac gaagatctcc tcagactttg tggtggtcga ctgcatgttc
     2581 catcccgccg acgttccgga cggcgaacag ttcgcggtga atgaaagcgg aaaattgcga
     2641 cacgaacacc ccctggtgtc cgtgatccgc aatcctcagc cgatcaccgt gatcaataag
     2701 gatccgatca ttcacaacag ccaggtgttt cagagtgaac ggggcaacat cgtcctgaat
     2761 ttcccgatcc cggtttcaac cgagccgcgg ggcggcctga tccatcttca gcccggcaaa
     2821 cggatcgccc agatgatctg cgggatgcat gaattcatgc aaagctggag cttcgcggtc
     2881 gacaatccct actacgcgaa gactcgaaag aacggcgagt tcaccatcga ccgggttcct
     2941 ccgggcacgt accggatggt ggcgtggcac ccgcatctgg cggtgatcca acggga
//
LOCUS       DBNGTT010000027         2756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2560499, whole genome shotgun sequence.
ACCESSION   DBNGTT010000027 DBNGTT010000000
VERSION     DBNGTT010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2560499"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..704)
                     /locus_tag="AC16BC_00565"
     CDS             complement(132..704)
                     /locus_tag="AC16BC_00565"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF014054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate--pyruvate lyase family protein"
                     /protein_id="HIR3240130.1"
                     /translation="MIPRVIALATTSIIWKTFEEFLADAESADVAPVLRLLLASDGSM
                     TTALEALRLGRIGVEVLRQGEEPIDDDTARRLGVAARQTAVSRHAWLTHAGERLLYAV
                     SVLPLESLDPDIAAEVRRGIEPLGRLFDTANRAVLRDGLRIGRLHDPDLAAAFNVAPS
                     ETLWCRTYRLSVERTLTASIVEVLSPRLAE"
     gene            complement(726..2651)
                     /locus_tag="AC16BC_00570"
     CDS             complement(726..2651)
                     /locus_tag="AC16BC_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240131.1"
                     /translation="MSDLKRLPAFAGIGPWLVLTALLYAPFLGDPFQYDDEHGIVLNA
                     AIQQPDAWKAALTGTLRSSAEIRSGHYRPLTYLTYWLTLRLSGLSPGAFHAVNLGLHV
                     IVAWLIVILARELTHDQRQAVLAGALFALHPAVSEAVLYASARATLLSSLFMLASLVC
                     FVRARRPSVPTAYEKIPPGPPFIKGGWGDLWRSTAKWWLGWAGFGLAALLSKESAVAL
                     PLLCLAADHCVLGSHHAASRRSRWWPHVVSFSGLIVFVAWLGLWRYVVQGLTSSDAFW
                     RYAGIVGNQVAAIGLAIRLFLVPWPLTVEHPLPAWPELAAVGLIALAGLCAAGGLIGL
                     ASPRPAARRAGFFALWAVIVALPTALWPLNVPFQEHRAYLQHAGLAMLAAPGLVAAWD
                     AGRVKRTATAATVIVIAAAWGWLIVDQGRAWSDPVRLWERARQHAPASFRAHTNAGLA
                     LAGADRWDDASRALDAALAINADYPPALVARGALAQRAGQSAAARADYERAVALRPDY
                     VPALFNLGLLSQASNDPASAEQWYRRALAVNPLHVDSLLNLGVLLLTRERFEEADALF
                     ASARSVRPDSPEALYYSGVIADRAGRIRDAEAFYHRAGREAALAGKRELAAEADARAR
                     RLADDRATSRAPERGTP"
BASE COUNT          468 a          919 c         1007 g          362 t
ORIGIN      
        1 gcagcagggt gccgtactgt ttgggcagac gaatgaggtc cgagaccgcg accagtttgg
       61 cgcgaacctt ggacgcgcgg gccgcgccgc ggtagtcgag accgtgggta ggggccgcgc
      121 cggtcgccac gtcactccgc caggcgggga gacaacactt ccacgatgga ggccgtgagc
      181 gtgcgttcga ccgacagccg gtacgtccgg caccagagcg tctcggacgg ggcgacgttg
      241 aaggcggcgg cgaggtcggg atcgtgcagg cgcccgatgc ggaggccgtc gcgcaggacc
      301 gcgcgattcg cggtgtcgaa cagccggccg agcggttcga ttccccggcg gacttcggcg
      361 gcgatgtcgg ggtcgagcga ttccagcggc aacacggaca ccgcgtacag caggcgttcg
      421 cccgcgtgcg tcaaccacgc gtggcgcgac accgcggtct ggcgcgcggc cacgcccagc
      481 cttcgcgcgg tgtcgtcgtc gatgggctct tcgccctggc gcagcacctc cacgccgatc
      541 cggcccaggc gcaaggcctc gagcgcggtg gtcatggacc cgtcggaggc gagcagcagt
      601 cgcagcaccg gggccacgtc cgcggattcg gcgtcggcga ggaattcctc aaacgttttc
      661 cagatgatgg aggtcgtggc gagcgcgatc acgcgcggga tcatacgtga ggattttgag
      721 gcgtgtcaag gagttccgcg ttcgggagcg cgcgacgtcg cccggtcgtc cgccagccgt
      781 cgcgcccgcg cgtcggcctc ggccgcgagt tcgcgcttgc cggcgagggc ggcttcgcgc
      841 cccgcgcggt gatagaacgc ctccgcgtcg cggattcgtc ccgcgcggtc ggcgatcacc
      901 cccgagtaat agagggcttc cggggagtcc ggccgaaccg agcgggcgga cgcgaacagg
      961 gcgtcggcct cttcgaaccg ttcccgcgtg agcagcagga cgccgaggtt cagcaggctg
     1021 tcgacgtgca gcgggttgac ggccagggcg cggcggtacc actgttccgc ggaggcggga
     1081 tcgttcgagg cctgggacag caggcccagg ttgaacaggg cgggcacgta gtcgggccga
     1141 agggcaacgg cgcgttcgta gtccgcgcgg gccgcagccg attgaccggc gcgctgagcc
     1201 agcgcgccgc gcgcgaccaa cgcgggggga taatccgcgt tgatggccag cgcggcatcc
     1261 agcgcgcgcg aggcgtcgtc ccagcgatcc gcgccggcca acgccaaccc cgcgttggta
     1321 tgggcgcgaa acgacgcggg cgcgtgctga cgcgcgcgtt cccagagccg caccgggtcg
     1381 ctccacgcgc ggccttggtc cacgatcagc cagccccacg ccgcggcgat cacgatcacg
     1441 gtcgcggccg tcgcggttcg cttcacgcgt cccgcgtccc acgcggcgac gaggcccggc
     1501 gcggcgagca tggcgagccc ggcgtgttgg aggtacgcgc ggtgttcctg gaacgggacg
     1561 ttgagcggcc acagcgcggt gggcaatgcc acgatgaccg cccataacgc gaagaagccg
     1621 gcgcggcgcg cggcgggccg gggggaggcg agtccgatca accctcccgc cgcgcacaac
     1681 cccgccagcg cgatcaggcc gaccgcggcc agctccggcc aggccgggag gggatgttcg
     1741 accgtgagcg gccagggaac cagaaacagt cggatggcca ggccgatcgc cgcgacctgg
     1801 ttgcccacga tgcccgcgta tcgccagaac gcgtcggagc tggtcaagcc ttggacaacg
     1861 tatcgccata atccgagcca cgccacgaac acgatcagcc cgctgaacga cacgacatgg
     1921 ggccaccatc tgctccgccg cgacgcggca tggtgggagc cgagtacgca gtggtccgcg
     1981 gccaggcaga gcagggggag cgcgaccgcg gactctttgg acagcagggc ggccagcccg
     2041 aacccggccc agcccagcca ccacttcgcc gtgctgcgcc acaaatcccc ccatcccccc
     2101 ttgatgaaag gggggccagg ggggattttc tcgtacgccg tggggacgga cggccggcgc
     2161 gcgcggacaa aacacaccag cgacgccagc atgaacaggg acgacaacaa cgtggcgcgg
     2221 gcggacgcat acaacacggc ctcgctcacg gccgggtgca gggcgaacag ggccccggcc
     2281 agaaccgcct gccgttggtc atgggtcaac tcccgggcca ggatcacgat gagccacgca
     2341 acgatcacat ggaggcccag gttcacggcg tggaacgccc ccggactgag gccgctcagg
     2401 cggagggtga gccagtacgt cagatacgtg agggggcggt agtgcccgct ccggatctcg
     2461 gcgctggagc gcagggttcc ggtcagggcc gctttccagg catccggctg ttgaatcgcg
     2521 gcgttgagga cgatgccgtg ctcatcatca tattggaacg ggtcgccaag aaacggggcg
     2581 tagagcaggg cggtcagaac gagccacggt ccgattcccg cgaacgcggg aaggcgcttc
     2641 aagtcggaca tgctgccact ataccccaat gtcttgagaa atccagggtc tcaccgggtt
     2701 cccggcgtgg cacgacttgg gcaatagtgg aacgaaaggt tccttctgtt ccgaaa
//
LOCUS       DBNGTT010000028         5051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_261400, whole genome shotgun sequence.
ACCESSION   DBNGTT010000028 DBNGTT010000000
VERSION     DBNGTT010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_261400"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="AC16BC_00575"
     CDS             complement(<1..1273)
                     /locus_tag="AC16BC_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3240132.1"
                     /translation="MCASVMSGVGDTYLGAFAIFLHASNSQVAFLTAVPQLLGAWVQF
                     VSVRVLHQWQRRKPLIIAGVLGQALTWLLIIATPFLFDQGGVWWLLLGATCYQLLGNF
                     VLPPWNSLMGDLVHPNQRGRYFGRRNRAMSVAAFTALCLGGIVLHQAEHERSVVLGFG
                     SVFAIALAARLASAYFLSRMVEPPFAATEEDRFSLWQFLRDGRRTNFGRFVGYVGSMH
                     FAVQVSGPMIAPYLLRDLRFTYLEFMLSTAAVVMAQFLTLPWWGRCCDRFGNLRVLTL
                     TGLLLPVIPVFWLFTTSVPGIMAVQMFAGASWAGFALAMGNFIFDNVKPAKRPQCVAV
                     YSAANAGGVFLGASLGGILVLWLPSAISVGGIHVKLTSNLQMLFLLSAVLRLAVSLKF
                     LPMIRETRDVAPFDAGEFSPRFLVVGWQTSAR"
     gene            complement(1456..2247)
                     /locus_tag="AC16BC_00580"
     CDS             complement(1456..2247)
                     /locus_tag="AC16BC_00580"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3240133.1"
                     /translation="MTDSWWLPVLLGVVEGLTEFLPVSSTGHLILTGALLGFTGEFAK
                     TFEVAIQLGAILAVVAYFRERLSGLVRGLSNDPGARAVTIAVAAAFLPAATVGFLAHG
                     AIKAYLFNTQTVAVSLIIGGVAILAIEARRTDGGVSQLESVNLRAALWVGVAQCFSLI
                     PGVSRAGATIMGGLLAGLDRKTASEFSFFLALPTMFAATLYDGYKSRALLVEENLVWL
                     ALGFASAFITALLVIAGFMRFIQRHTFRPFAYYRIVVGGALLLLS"
     gene            complement(2244..3539)
                     /locus_tag="AC16BC_00585"
     CDS             complement(2244..3539)
                     /locus_tag="AC16BC_00585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3240134.1"
                     /translation="MVPSFAVAVGVGALGGWLAGRWLAERLAPLISAASDLAEGRSAL
                     VPHDESTPVLDEIGRLTAALRAAAEGMERRVAATTEDRTQFGAVLSGMVEGVLVLDRA
                     GRILLLNPAMERMLGDRAARTIGRHWLEVVRQHDLNELIRAVILHAEPRAATITLDDP
                     AGDKTFAVQGSVARRPDGGADDLRAVFVFHDVTVLKRLERVRADFIANVSHELRTPLT
                     AIIGYLEALLDGAQSDPRQHDEFLRTIKTHADRLAALVHDLLQLSQIESGDYRWRRES
                     VDVVALVRRTVELIQPLAQRRHIAVRWASDEAAARVVADEEKLTQVFVNLLDNAVKYT
                     EPGGAVDATVTASDTRVVIRVRDTGIGIAPADRDRVFERFYRVDRARSRESGGTGLGL
                     SIVKHVVEAHGGAVTVDSQLGQGTTFTITLPRDREHHTV"
     gene            complement(3643..4341)
                     /locus_tag="AC16BC_00590"
     CDS             complement(3643..4341)
                     /locus_tag="AC16BC_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3240135.1"
                     /translation="MKTALVVEDEPDIVRLLSHYLAQAGYRVRTESDGLSGLSAARED
                     VPAVIILDVMLPGLDGYEVCRRLRSDAATAAVPILMLTARGAETDKVRGLEIGADDYV
                     TKPFSPKEVVARVKALVRRSGGEAAGSRLSYGDLTLDTERHEVSVHGREVSLTAKEFA
                     LLEYLLRRRGKLATREALLNSVWGYDADVTTRTVDVHIRRLREKIPSLTTAIQTVKPY
                     GYKLAETPADAADA"
     gene            complement(4338..4715)
                     /locus_tag="AC16BC_00595"
     CDS             complement(4338..4715)
                     /locus_tag="AC16BC_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240136.1"
                     /translation="MIPSVYIEVEDNPNCPGREPMRFRELGAAAVVAKVRVFDRRDEG
                     EWCLVTGVGKNAAQPWEPAHVRKVEDSGVGVAYLIYGGLWGIRLHPVDVARPWRFDDA
                     AQWGEPYLLLTDATDIVTTSSPP"
     gene            4846..>5051
                     /locus_tag="AC16BC_00600"
     CDS             4846..>5051
                     /locus_tag="AC16BC_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014693.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HIR3240137.1"
                     /translation="MEKSFDDVVDFHRHLCLDIAMGYRVGKALVREMGKELRNMTDVF
                     AIVENETCAVDAIQEFTGCTLGKR"
BASE COUNT         1015 a         1721 c         1558 g          757 t
ORIGIN      
        1 cccgggcact ggtctgccag cccacgacca gaaacctggg agaaaactcg cccgcgtcga
       61 atggcgcgac gtcgcgcgtc tcgcggatca tcggcaagaa cttcagcgat accgcaagcc
      121 gcaacacggc tgagagcagg aacaacattt gcaaattcga ggtcagtttg acgtggatgc
      181 cgccaaccga gatcgcgctg ggcagccaca acaccagaat cccgccgagc gaggcgccca
      241 ggaatacccc accggcgttg gccgcgctat agaccgccac gcactgcggg cgtttggccg
      301 gtttgacgtt gtcgaagatg aagttgccca tggcgagggc aaaccccgcc caactcgcgc
      361 ccgcgaacat ctgcaccgcc atgatgccgg gcacgctggt ggtgaacagc cagaacacgg
      421 gaatgacggg caacaggagc ccggtgagcg tgagcacgcg caggttcccg aaccgatcgc
      481 aacatcgccc ccaccacgga agcgtcaaaa actgcgccat gaccacggcc gcggtcgaca
      541 gcatgaactc caggtacgta aaccgcaggt cgcgaagcaa gtatggcgcg atcatgggcc
      601 cggagacttg caccgcgaag tgcatcgacc ccacgtatcc gacaaaccgg ccgaagttgg
      661 tgcggcgacc gtctcgcaga aactgccaga gcgaaaagcg gtcctcctcg gtagccgcaa
      721 agggcggctc caccattcgc gacaaaaaat acgcggaggc caggcgggcc gccagcgcga
      781 tcgcgaacac cgacccaaat cccagtacga ccgagcgttc atgttcggcc tgatgcagca
      841 cgatgccgcc gaggcacagc gcggtgaacg cggccacgct catggcgcgg ttgcgtcgcc
      901 caaaatatcg cccacgctga ttggggtgga cgagatcgcc catcagactg ttccaggggg
      961 gcaagacgaa attcccgagc agctggtaac acgtggcgcc caacaatagc caccagacgc
     1021 cgccctggtc gaacagaaac ggcgtcgcga taatcagcag ccacgtcaac gcctgtccca
     1081 ggacacccgc gatgatcaac ggcttgcgcc gctgccattg gtgcaacacg cgcaccgaca
     1141 cgaactgaac ccacgcgccc agcaactggg ggaccgcggt gagaaacgcg acctgcgaat
     1201 tcgacgcgtg cagaaaaatg gcgaacgcgc cgagataggt gtcgcccacc ccggacatga
     1261 ccgacgcgca caccccatcc cgcgtggaat gcttgagact tcggcgaacc tcgttgtgcg
     1321 atggtggttc gacggctatt ggactggaag cgtgttccaa cgacatgacg ttaacgggaa
     1381 ccggacccga cgtgacctct cgctgcacgc cagtataaca aaccggaggg ctgaggccgg
     1441 ctagcctcgt ccgactcacg acaacaggag gagggctccc ccgaccacga tccggtagta
     1501 ggcgaacggg cggaacgtat ggcgctggat gaatcgcatg aacccggcga tgaccagcag
     1561 cgcggtgatg aacgcggacg caaatccgag agcgagccag acaaggttct cttcgaccaa
     1621 caatgcgcgg ctcttgtagc cgtcgtacag cgtggccgcg aacatcgtgg gaagcgccag
     1681 gaagaatgag aactccgacg cggttttgcg gtccagaccc gcgagcagac cgcccataat
     1741 ggtggcgccc gcgcgcgaga cgccgggaat gagggagaaa cactgcgcca cgccgaccca
     1801 gagcgcggcg cgcaaattca ccgattcgag ttgcgaaacg cctccgtcgg ttcgacgggc
     1861 ttcgatcgcc aggatcgcca cgccaccgat gatcagggag acggcgacgg tctgcgtgtt
     1921 gaacaagtac gctttgatgg cgccgtgggc caagaatccg accgtggccg ccggcagaaa
     1981 cgcggcggcc acggcaatcg tgaccgcgcg ggctccggga tcattcgaca agccccgcac
     2041 taatccggac agccgctcgc ggaaatacgc cacgaccgcg aggatcgcgc ccagctggat
     2101 cgccacttcg aatgttttgg cgaactcgcc cgtgaacccc aacaacgcgc cggtgagaat
     2161 caagtggccg gtggatgaca ccggcaagaa ttccgtcagc ccttccacca cacccagaag
     2221 gacgggcaac caccaggaat cggtcatacc gtgtgatgct ccctatcgcg aggcaacgtg
     2281 atggtgaagg tcgtgccctg ccccaattgg ctgtcgacgg tcacggcccc gccgtgcgcc
     2341 tccaccacgt gtttaacgat cgacagaccc aagccggtcc cccccgactc gcgcgagcgc
     2401 gcgcggtcca cgcgatagaa ccgctcgaac acgcgatcgc ggtccgcggg ggcgatcccg
     2461 atgcccgtgt cgcgcacgcg gatcacgacc cgcgtgtccg acgcggtcac ggtcgcgtcc
     2521 actgcgccgc cgggctcggt gtatttcacc gcgttgtcga gcaggttcac gaagacctgg
     2581 gtgagtttct cctcgtccgc caccacccgc gccgccgctt catcgctcgc ccatctcacc
     2641 gcgatgtggc gccgctgggc aagcggctga atcagctcca ccgtccggcg caccaaggcc
     2701 accacgtcca cggactcccg ccgccaccgg tagtccccgg actcgatctg ggagagttgc
     2761 agcaggtcgt gaaccagcgc ggcaagccgg tccgcgtggg tcttgatggt gcgaaggaac
     2821 tcgtcgtgtt gccgcgggtc cgactgcgcg ccgtcgagga gcgcctccag gtacccgatg
     2881 atcgcggtca gaggggtgcg gagttcgtgc gagacgttcg cgataaaatc cgcgcgcact
     2941 cgctcgagtc tcttgagcac cgtgacgtcg tgaaacacga acaccgcgcg caggtcgtcc
     3001 gcgccgccat ccggccgccg cgccaccgat ccctgcaccg cgaacgtctt gtcgccggcc
     3061 ggatcatcga gcgtgatcgt tgcggctctg ggctcggcat ggagaatcac ggctcgaatg
     3121 agttcattca ggtcatgttg acgcacgacc tccagccagt gacggccgat ggtccgggcc
     3181 gcgcgatcgc cgagcatgcg ttccattgcg ggattgagca acaggatccg gccggcgcgg
     3241 tccaggacga gcactccctc caccatcccg gacaacacgg cgccgaactg cgtacggtct
     3301 tcggtggtcg cggccacgcg acgctccatc ccctccgcgg cggcgcgcaa cgccgcggtc
     3361 aggcgcccga tttcgtcgag gaccggcgtt gattcatcgt gcgggacaag agccgaacgg
     3421 ccctccgcaa gatccgacgc cgccgagatc aaaggcgcca accgctccgc cagccaccga
     3481 cccgccagcc acccgcccaa ggcgccgacg cccacggcca ccgcgaatga cgggaccacc
     3541 cagccgcgac tcccggggcg ccgacctgac ccactacgaa ggcgaccgcg gcgatcccgg
     3601 ctccggcccc ggcgccgacc acggcgatcc gcaccgtggt cgtcatgcgt cggccgcatc
     3661 agccggcgtt tcggccaact tgtacccata gggcttgacc gtctggatgg ccgtagtcaa
     3721 cgacgggatt ttttcgcgca ggcgtctgat atggacgtcg acggtgcggg tcgtcacgtc
     3781 ggcgtcatac ccccacaccg agttgaggag cgcctcgcgg gtggccagtt ttccacgacg
     3841 gcgcaacaaa tactcgagca acgcgaattc cttggccgtg agcgacacct cgcgaccgtg
     3901 caccgacacc tcgtgccgtt cggtgtccaa tgtcaggtcg ccatagctca accgggaccc
     3961 ggcggcttcc ccgcccgaac gccggaccaa ggctttgacg cgcgccacga cctctttcgg
     4021 gctgaagggc ttggtcacgt agtcgtccgc gccgatctcc agaccgcgga ccttgtcggt
     4081 ttccgcgccg cgggcggtca gcatcaagat cggcaccgcg gccgtggcgg cgtcgctccg
     4141 gaggcgccgg cacacctcgt acccgtccag gcccggcagc atgacgtcaa ggatgatcac
     4201 ggccgggacg tcttcgcgag ccgcgctgag ccccgacaag ccgtcagatt cggtccggac
     4261 acggtacccg gcctgggcta gataatggga gaggagacga acgatatccg gttcgtcctc
     4321 gacaacgagc gcggttttca cggcggagag gacgtggtca cgatatccgt ggcatcggtt
     4381 aagagcaaat agggttcgcc ccattgagcc gcgtcgtcga accgccacgg cctcgcgaca
     4441 tcaaccggat ggagccggat cccccagagg cccccataga tcaggtaggc gacccccaca
     4501 cccgagtcct ctactttccg cacgtgcgcc ggctcccacg gctgggccgc attcttcccc
     4561 accccggtca ccagacacca ctctccctcg tcgcggcgat cgaacacgcg aactttggcg
     4621 acaaccgcgg cggcccccaa ttcacggaac cgcatcggct cccgccccgg acaattggga
     4681 ttgtcctcga cttcaatgta cacggacggg atcatagcag tccgaatccg ctggatcaat
     4741 caacgattgc cttcacgccg cggcatcccc tatacttgga tttattcgtc atgcgtccgc
     4801 cgtcaagggt cgctgcggcc cgccggctca ctctgagagg ttctgatgga aaaatcgttc
     4861 gacgacgtgg tggattttca ccggcacctg tgtctcgata ttgcgatggg gtatcgcgtc
     4921 ggcaaggcgc tggtgcggga aatgggcaag gaacttcgca acatgacgga cgtgttcgcg
     4981 atcgtggaaa acgagacctg tgcggtggac gccatccagg agttcacggg ctgcacgctg
     5041 ggcaagcgca a
//
LOCUS       DBNGTT010000029         4766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1666763, whole genome shotgun sequence.
ACCESSION   DBNGTT010000029 DBNGTT010000000
VERSION     DBNGTT010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1666763"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="AC16BC_00605"
     CDS             <1..511
                     /locus_tag="AC16BC_00605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3240138.1"
                     /translation="DPEARRKIRRMKDQIAGVSADAHGLARKLHPAILDDLGLVAAIE
                     SECLGVSKRDGIRIAFAAERIPNRLPKDVSLALYRIAQEGLRNMAKHARTERAVVRLV
                     SAGDTVRLVIQDFGVGFTPRTVRGQGGLGLESIRERIRLVQGQMSIKSRPNRGTVIAV
                     EVPLKRSET"
     gene            508..1155
                     /locus_tag="AC16BC_00610"
     CDS             508..1155
                     /locus_tag="AC16BC_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3240139.1"
                     /translation="MRRARVIVADDHRMVTEGLRGVIEREFDLVEIVEDGQALLKAVQ
                     RQRPDLVVLDVSMPGLNGIDAAEQLRKHDPNLKIVFLTMHRDVLYVKRAFENGASGYV
                     LKHSAPAELITAMREALEGRTYVTPTLVGDASELLTDRSFSRRPRPSALRLTPRRREV
                     LQLVAEGRSAKEVASILHISPRTVEYHKYRIMDDFNLRTTADVIQFALRHGLGSI"
     gene            1234..1905
                     /locus_tag="AC16BC_00615"
     CDS             1234..1905
                     /locus_tag="AC16BC_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HIR3240140.1"
                     /translation="MRVLIVEDDDKAAAYLKKGLAENGFVVDVAGRGEEGLHLLTTGR
                     YDLAILDVMLPERDGWSILAELRRRGNQTPVLFLTARDAIKDRVKGLELGADDYLVKP
                     FAFSECLARIRSILRRGPGRQPEVVRVDNLELDLLQHKAVRGGKALELTPKEFNLLSL
                     LARRKGEVLSRTLIAEQVWDMNFDSDTNVVDVAVRRLRSKVDDPFARKLIHTVRGVGY
                     VLDER"
     gene            1895..3322
                     /locus_tag="AC16BC_00620"
     CDS             1895..3322
                     /locus_tag="AC16BC_00620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HIR3240141.1"
                     /translation="MSADPPDKRVHHRSAIGSWSITTRLTILYAVSTFGLLVVATMFL
                     YWMMGADLDLADRKLLSERAQILWTILQEFPDNQSALVEELGLGAYDARLLDDTGRTL
                     LETPGMSARLPADRFPPPATYDRKPKAAVSKWGNGAQSYLMMSLWVPVGRAGQPQAVL
                     QIALDVSSEDAIAAGYRRRLVVVLVAGLLFAAAAGATVARRGMRPLAEITRAATRISA
                     AQLHERVNPAQWPRELTALATAFDQMLDRLEASFARLSQFSADLAHELRTPINNLMGE
                     ADVALSRVRTPEEYRKVLESSLEEFAKLSRTIDGLLFLARADHPDTQIQRAALDARKE
                     IEAVREFFDAMAQEQGVEVVCDGDATLRADPLLFRRAVSNLLSNALQYTPRGGRVVMS
                     AAGSPDRGAVIRVRDTGLGIEAEHLKKIFDRFYRVDRARSHFPRGVGLGLAIMKSIMD
                     LHHGVVTVDSEPGKGTTVSLQFPPA"
     gene            3454..3816
                     /locus_tag="AC16BC_00625"
     CDS             3454..3816
                     /locus_tag="AC16BC_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240142.1"
                     /translation="MTKWSRALGLLAVPVLLAGVPAMAAPTHMLAMTGQIRKVDATAH
                     TVTVAGLPGKKEHQMTFHLASDAKITRNEQDVPVNDLKIGDRVTVHYQKEKRQAMAHT
                     ITLTAAEAEPAKLGTYGK"
     gene            3953..>4766
                     /locus_tag="AC16BC_00630"
     CDS             3953..>4766
                     /locus_tag="AC16BC_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3240143.1"
                     /translation="MWVSTVKPGNEREHESANRTSRDASLRAAVVLAGGDGRRLESYV
                     RRLRGDALPKQYVTLYGTQSLLERTFKRVERLVPPERIMTVVNHDHLQYPDACRQLAG
                     RPANTVVVQPRNRDTGPGLLLPLAYLQRRCEDAVVAVFPSDHYVSNDRALMQHVETAC
                     RAVEERPDVIVLLGVSPNAPETDYGYVLPGSSMDADGAPRIRSVRRFIEKPTARQAHD
                     LIEQGALWNTSILVFRLPMLLDLMWSVAPLLSLRFTEIVDAIGTPRAHDVIDA"
BASE COUNT          816 a         1545 c         1647 g          758 t
ORIGIN      
        1 ggaccccgag gcgcgccgga agatccggcg catgaaggat caaatcgcgg gcgtgtccgc
       61 ggacgcgcac ggattggccc ggaagctcca ccccgctatc ctggatgatt tggggctggt
      121 ggccgccatc gagtccgaat gtctgggcgt gtcgaagcgc gacgggattc ggatcgcgtt
      181 cgcggccgag cgcatcccga accgactccc gaaggacgtg tccctcgcgt tgtaccgcat
      241 cgcgcaggag gggctgcgca acatggccaa gcacgcgagg acggagcgcg ccgtggtgcg
      301 gctcgtgagc gcgggggaca cggttcgatt ggtcattcag gacttcggcg tcgggttcac
      361 gccccgcacg gtgcgggggc agggcgggct ggggttggag agcatcaggg agcgtatccg
      421 gttggtccag gggcaaatgt cgatcaaatc gcggccgaac cgcggcacgg tgatcgcggt
      481 cgaggttccg ttgaaaagga gcgagacatg aggcgagcgc gcgtaatcgt ggcggatgat
      541 catcgcatgg tgaccgaagg tctgcggggc gtcatcgaac gcgaattcga tctggtcgag
      601 atcgtggaag acgggcaggc gttgctcaaa gccgttcagc ggcagcgccc ggacctggtg
      661 gtgctggacg tgtccatgcc cgggctgaac gggatcgacg ccgcggagca gctccggaaa
      721 cacgacccga acctcaagat cgtgttcctg accatgcatc gggacgtgct ctacgtgaaa
      781 cgcgcgttcg aaaacggcgc gtcgggctac gtcctgaagc actcggcccc ggccgaattg
      841 atcaccgcga tgcgggaggc gctggaggga cgaacctacg taacgcccac gttggtcggc
      901 gacgcctccg agctgttgac cgatcggtcg ttcagccgtc gcccccgacc cagcgccctc
      961 cggctcacgc ccagacgccg cgaggtgctg caactggtgg ccgagggccg gtccgccaaa
     1021 gaggtcgcct cgatcctcca catctccccc cgaaccgtgg agtaccacaa gtaccggatc
     1081 atggacgact tcaacctccg aaccaccgcg gacgtgatcc agttcgcctt gcggcacggc
     1141 ctcgggtcca tctgaccccg gccgccgtga ggcggccccg agtccgggcg ttcttgtctc
     1201 acgaggattc gcggcggtag aattcacggc gacatgcgcg tcctcatcgt cgaagacgac
     1261 gacaaagccg cggcctattt gaaaaagggg ctggcggaga acgggttcgt ggtggacgtg
     1321 gcggggcggg gcgaagaggg gctgcacctg ctcaccaccg gccggtacga cctggccatt
     1381 ctcgacgtga tgctgccgga gcgcgacggc tggtccatcc tggccgaact ccggcgccgc
     1441 ggcaaccaga ccccggtcct gttcctcacc gcccgcgacg ccatcaagga tcgcgtgaaa
     1501 ggcctggaac tcggcgcgga cgactacctg gtcaaaccgt tcgcgttctc cgagtgtctc
     1561 gcccgcatcc ggtccatcct gcggcgcggg cccggccggc aaccggaagt ggtgcgcgtc
     1621 gacaacctgg aactggatct gctgcagcat aaggcggttc gcggcggcaa ggccctggag
     1681 ctgaccccca aggagttcaa cctcctctcg ctgctggcgc gtcgcaaggg cgaagtcctg
     1741 tcgcggaccc tgatcgcgga gcaggtgtgg gacatgaact tcgacagcga caccaacgtg
     1801 gtggacgtgg cggtccgccg gttgcggagc aaggtggacg accccttcgc caggaagctc
     1861 atccacaccg tgcgcggggt cgggtacgtg ctcgatgagc gctgatccgc cggacaaacg
     1921 cgtgcatcat cgatccgcga tcggctcctg gtcgatcacc acgcgcctca ccatcctcta
     1981 cgcggtctcc accttcgggc tgctggtggt ggccacgatg tttctctact ggatgatggg
     2041 ggccgatctg gacctggccg accgcaagct gctcagcgaa cgcgcgcaga ttctctggac
     2101 gattctgcag gagttccccg acaaccagag cgcgttggtg gaggaactcg gactcggggc
     2161 ctacgacgcg cgcttgctcg acgacaccgg gcggaccctg ttggagacgc cgggcatgag
     2221 cgcgcgcctt cccgcggacc ggttcccgcc cccggccacg tacgatcgaa agcccaaagc
     2281 cgcggtgagc aagtggggga acggcgcgca gtcctacctc atgatgtccc tgtgggtgcc
     2341 ggtggggcgc gcggggcaac cgcaggcggt cctgcagatc gcgctcgacg tgtccagcga
     2401 ggacgcgatc gcggccgggt accgccgccg gctggtcgtg gtgctggtcg cgggcctgct
     2461 cttcgcggcc gcggccgggg ccaccgtggc ccgccgcggc atgcgccccc tggcggaaat
     2521 cacgcgcgcg gccacgcgca tcagcgcggc gcagttgcac gagcgcgtga acccggcgca
     2581 atggccgcgc gaactcacgg ccctggccac ggcgttcgac cagatgctcg accgcttgga
     2641 ggcgtccttc gcccggttgt cgcaattctc cgcggatctg gcccatgagt tgcgcacgcc
     2701 catcaacaac ctgatgggcg aggccgacgt ggcgctctcc agggtgagga cgccggagga
     2761 gtaccggaag gtgctcgaat ccagcctgga ggaattcgcc aagctctccc gcaccatcga
     2821 cggactgctc tttctggccc gcgccgatca tccggacacg cagatccagc gcgcggcgct
     2881 ggacgcgcgc aaggagatcg aggcggtccg ggaattcttc gacgccatgg cgcaggagca
     2941 gggcgtggag gtggtctgcg acggggacgc cacgttgcgc gcggacccgt tgttgttccg
     3001 gcgggcggtc agcaatctgc tctccaacgc gctccagtac acgccgcggg gcgggcgggt
     3061 cgtgatgtcc gcggccggtt ccccggaccg cggggccgtg attcgcgtgc gcgacaccgg
     3121 gttaggcatc gaggcggaac acctgaaaaa aatcttcgac cggttctacc gagtggatcg
     3181 cgcccggtcc catttccccc gcggcgtcgg gctcgggctc gccatcatga agtccatcat
     3241 ggatctccat cacggggtcg tgacggttga cagcgaaccc ggcaaaggca ccacggtctc
     3301 gttgcagttt ccccccgcgt agtccgtttt caccccgttt ccgaccacgt cgtcagtttc
     3361 cgcagatgac agtcttgtca tgcggccgtc atgttcccga cagtcgtggg tgcgtacact
     3421 ggcgtcgacg acttggggaa ggaggtgata gagatgacga aatggtctcg agcgctcggg
     3481 ctcctggcgg tgcccgttct gctggccggc gtaccggcca tggcggcgcc cacgcacatg
     3541 ctggccatga ccgggcagat ccgcaaagtg gacgcgacgg cgcacaccgt gaccgtggcg
     3601 gggctcccgg gcaagaagga acaccagatg acgtttcatc tggcgtccga cgcgaagatc
     3661 acccgcaatg agcaagacgt tccagtgaac gacttgaaga tcggtgatcg ggtgaccgtg
     3721 cactaccaga aagagaaacg gcaagcgatg gcgcacacca ttacgctgac agcggccgag
     3781 gccgagccgg ccaagctcgg cacgtacggg aagtaatcat ccggccgtaa cggccggagc
     3841 ggacaggcgg ggagggcagg gcgacacgcc ttgccctccc tccgggaggc ggcgggtggc
     3901 gacggggtgt cggcaaatgg gcgagttgct cgaaacggac aggaggtctg gcatgtgggt
     3961 gagcacggtt aagccgggga atgagcgaga gcacgaatcg gcaaaccgca cgtcgcggga
     4021 cgcgagcctt cgcgccgcgg tggtgctggc cggaggcgac ggccgtcgcc tcgaatcgta
     4081 cgttcgtcga ttacggggcg atgcattgcc caagcagtac gtgaccctct acgggacgca
     4141 atccctgctg gagcgcacgt tcaagcgggt ggaacgcctc gtgcccccgg aacgcatcat
     4201 gaccgtggtg aaccacgatc acctccaata cccggacgcg tgccggcaac tggcgggccg
     4261 tcccgcgaac accgtggtcg tgcaaccccg gaaccgggac accggcccag ggcttctgct
     4321 gccgctcgcc taccttcaac ggcggtgcga ggacgcggtg gtcgccgtgt ttccgtcgga
     4381 ccactacgtg agcaatgacc gggccctgat gcaacacgtg gagaccgcgt gtcgggcggt
     4441 cgaggagcgc ccggacgtca tcgtcctcct gggggtgagc cccaatgcgc cggaaaccga
     4501 ttacggctac gtgttgcccg gctcgtccat ggacgcggac ggcgcgccgc gcatccggag
     4561 cgtgcgtcgt ttcattgaga agccgaccgc gcgccaggcc catgacctga tcgagcaggg
     4621 cgcgttgtgg aacacctcga tcctggtgtt tcgtctcccc atgctcctgg atctgatgtg
     4681 gagcgtggcc ccgctcctgt ccctgcgctt cacggagatc gtggacgcca tcggcacgcc
     4741 ccgcgcgcac gacgtgatcg acgccg
//
LOCUS       DBNGTT010000030         2918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1155930, whole genome shotgun sequence.
ACCESSION   DBNGTT010000030 DBNGTT010000000
VERSION     DBNGTT010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1155930"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="AC16BC_00635"
     CDS             <1..488
                     /locus_tag="AC16BC_00635"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HIR3240144.1"
                     /translation="SAVGKRYRYRLLNRPTPSPLERRTSWHVPQRLNQTAMRRAAASI
                     VGRHDFRAFEGADRSHAPTRDTVCRLTRCAIRKQADVIAIEVEGNRFLKYMVRNIVGT
                     LVEIGLGRRSVNDITELLRTGDRRCAGITAPAHGLTLMEVQYASRSRKRPSRATGGAG
                     S"
     gene            complement(485..973)
                     /locus_tag="AC16BC_00640"
     CDS             complement(485..973)
                     /locus_tag="AC16BC_00640"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3240145.1"
                     /translation="MATEDEDKRLFRRVDVLIPFGFRPVGRGAVQSIVHTEKESFHLN
                     DDPVRAALDRIERKVDRLLARLESGDQTPPVQPKRINLSGSGVRFSSDDPLEEGQYLD
                     LAMVLPGESPVALRAVGEVVRQRLLARPSGAVYEIAVSFVDLAPADRESIIRYTFQAT
                     GR"
     gene            complement(963..2252)
                     /locus_tag="AC16BC_00645"
     CDS             complement(963..2252)
                     /locus_tag="AC16BC_00645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3240146.1"
                     /translation="MPDLSASLAIVHRLAGTLSGVFDLTSIGDAVVTVLTEELGLHSC
                     SVMILDGRRERLVNLAGAGAAAVAPGAPRRSFTLGEGIAGTVAVTGRAIRVNDARRDK
                     RFLDRSAAVRSLLCFPLFSGDQVIGVLNLSHPEKGFFTEEHEAVFAVLSTVVGHLVAF
                     AGLRAELAELNQSLESKVLAKTREIEAAQRRAFQQEKLAALGTLVAGVAHELNNKLVP
                     LVAYSHMLKDTLHDGEERRVMAAMAEGADAAKAIVDSLLRFARPEPVNLSVVDCNRIV
                     ADVTELLPQRVTRPEIEFRTEMEPNPQLVMGDARQIGQVLVNLINNGYDAMGGAGAIE
                     VRTRSRGPVIELEVADSGHGIPSEHLSKIFDPFFTTKDVGRGTGLGLSLCYGMVRAHR
                     GEIDVQTRPGRTVFTVRLPAADPAVAAGNEGARRYGY"
     gene            complement(2393..>2918)
                     /locus_tag="AC16BC_00650"
     CDS             complement(2393..>2918)
                     /locus_tag="AC16BC_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3240147.1"
                     /translation="RIFDQIEHMEKPVVAAINGLFCLGGGLELAMACHLRVAGERVRL
                     GQPEIDLGIIPGFGGTQRLVRLVGQAKAIELILTGDKIPGPEAKAIGLVNKVVPDGEV
                     VKQAQGLAKKIAGKSAVAVRAALRAIREGIGRPLPEGQALESQLFGMVCESADKREGV
                     SAFLEKRQPKFQDR"
BASE COUNT          514 a         1037 c          866 g          501 t
ORIGIN      
        1 tctccgccgt gggcaagcga tatcgctatc gtctcctgaa ccggccgacg ccgtcccccc
       61 tcgaacggcg aacctcatgg cacgtcccgc aacggctcaa ccagaccgcg atgcgccgcg
      121 ccgccgcgtc gatcgtcggg cggcacgatt ttcgggcgtt cgaaggcgcc gaccggtccc
      181 acgccccgac ccgagacacc gtgtgccgcc tcacgcgctg cgccattcgc aagcaggcgg
      241 acgtgatcgc cattgaggtg gaggggaatc ggtttctcaa gtacatggtg cgcaacatcg
      301 tcggtacgct cgtcgagatc ggcctcgggc gccggtcggt caacgacata actgaactct
      361 tgcggacggg cgatcgccgt tgcgccggga tcacggcgcc ggcccacgga ttgaccctga
      421 tggaagttca atatgcctcg cggtcgagga aacgtccgag ccgagcaacc ggcggcgccg
      481 gctcctagcg gccggtggcc tggaaggtgt agcggatgat ggattcccgg tccgcgggag
      541 ccaggtccac gaatgacacg gcgatctcat agaccgcccc ggagggacgg gccaacaacc
      601 gttggcgcac cacctccccg acggcgcgca gcgccaccgg agactctccc ggcaacacca
      661 tggcgagatc caaatactgc ccttcctcca acggatcgtc gcttgaaaac cgcaccccgg
      721 acccgctcaa attgatccgt ttgggctgaa cagggggcgt ctggtcgccg gattcaaggc
      781 gcgcgagcag ccgatccacc ttccgttcga tccgatccaa cgccgcacgt accgggtcgt
      841 cgttcaaatg aaacgactct ttctccgtgt gaacgatgga ctgaaccgct ccgcggccga
      901 ccggccggaa cccgaatggg atgaggacat caacgcggcg gaacaaccgc ttgtcttcgt
      961 cttcagtagc catatctcct cgccccctcg tttcccgccg cgaccgccgg atcagccgcc
     1021 gggagccgga ccgtaaatac cgtccggccc ggacgcgtct gcacgtcgat ctcgccccga
     1081 tgcgcgcgaa ccatgccgta acagagcgat agcccgagtc ccgttccccg gccgacgtct
     1141 ttggtcgtga aaaacgggtc gaaaatcttg gacaaatgtt cggatggaat cccgtgtccc
     1201 gagtccgcca cctccagctc gatgacgggg ccgcgggatc gcgtccggac ttcgatcgct
     1261 ccggcccccc ccatggcgtc atacccgttg ttgatcaggt tcacgagcac ttgtccgatt
     1321 tgccgcgcgt cccccatcac caactgcgga ttgggctcca tctcggttcg aaattcgatc
     1381 tcggggcgcg tgacccgttg cggcaacagt tcggtgacgt cggcgacgat ccggttgcag
     1441 tcgaccaccg acaggttgac cggttccggt ctggcgaaac gcagcaggct gtccacgatc
     1501 gctttcgcgg cgtccgctcc ctcggccatg gcggccatca cgcggcgttc ctccccgtcg
     1561 tgcagcgtgt ccttcaacat gtgggagtaa gccaccagcg ggaccagctt gttattgagt
     1621 tcatgcgcca cgcccgccac cagcgtgccg agcgctgcca gcttttcctg ttgaaaggct
     1681 ctccgttgcg cggcttcgat ctcccgggtc ttggccagca cttttgattc aagcgactgg
     1741 ttgagttccg ccagctcagc cctcagaccg gcaaacgcga cgagatgtcc caccacggtc
     1801 gacagcaccg cgaagaccgc ttcgtgctcc tcggtgaaga aacccttttc gggatgactg
     1861 aggttcaaga cgccgatgac ctggtcgccc gaaaacaggg ggaaacacag cagcgatcgc
     1921 acggcggcgg accggtcgag gaatcgcttg tcccgacgcg catcattgac ccggatcgcc
     1981 cgccccgtga ccgcgaccgt tcccgcgatc ccttcgccca gcgtgaacga ccgccgaggc
     2041 gctcccggag ccaccgcagc cgctccggcc cccgcgaggt tcaccagccg ctcgcgacgc
     2101 ccatccaaga tcatcaccga acaggagtgc aaccccagtt cctcggtcag cacggtcacg
     2161 accgcgtcgc cgatcgacgt cagatcgaac acgccggaaa gcgtcccggc caatcggtgg
     2221 acaatcgcaa gactggcgga cagatcgggc atcacgtccg gctcggtacg gagggacacg
     2281 cgtcgggccc cggacctcgt ggctgttgcc cgggaggacg tcacgggtcg acgccgttgg
     2341 ccggagctcg acgatcggcc actagacgca gagccccttc gtttgcgtgg ggtcaccggt
     2401 cttggaactt cggttgccgc ttttcgagga acgcgctcac accctcccgc ttgtccgcgc
     2461 tctcgcacac catcccgaac aactgggatt caagcgcttg cccctcgggc agcgggcgcc
     2521 cgatcccttc acggatcgcg cgaagggccg cccgaaccgc caccgcgctc ttccccgcga
     2581 tcttcttcgc gagcccttgc gcctgcttga cgacttcgcc gtccggcacc accttgttga
     2641 ccagcccgat ggccttggct tcggggccgg gaatcttgtc gccggtgaga atcaactcga
     2701 tcgccttggc ttggccgacc aggcgaacca accgctgcgt cccgccgaaa cccgggatga
     2761 tgcccagatc gatttccggc tgacccagcc gaacccgttc gccggccaca cgcaggtggc
     2821 aggccatcgc caactccagc cctcccccga ggcaaaagag gccgttgatt gcggccacca
     2881 cgggcttctc catgtgttcg atctggtcga agattcgt
//
LOCUS       DBNGTT010000031        14258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1539268, whole genome shotgun sequence.
ACCESSION   DBNGTT010000031 DBNGTT010000000
VERSION     DBNGTT010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 14258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14258
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1539268"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2749)
                     /gene="carB"
                     /locus_tag="AC16BC_00655"
     CDS             complement(<1..2749)
                     /gene="carB"
                     /locus_tag="AC16BC_00655"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR3240148.1"
                     /translation="MPKRTDIRKILLIGSGPIVIGQACEFDYSGSQACKALREEGYEV
                     VLVNSNPATIMTDPELAHRTYIEPITPEVVERIIERERPDALLPTMGGQTALNTAVAL
                     VERGVLEKYQVKLIGAQYDAIKKAEDRERFKQAVRRVGLKVPESGTVSSVEDAMAVIE
                     RVGFPAIIRPSFTLGGAGGSVAYNREEFKRAVEWGLDASPTHQILVEQSVLGWKEFEL
                     EVMRDTRDNVVIICSIENVDPMGVHTGDSITVAPAMTLTDKEYQVMRDAAIAIIREIG
                     VDTGGSNIQFAVNPENGDLAVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LPNDITRQTRAAFEPTIDYVVVKIPRFAFEKFPQADATLTTQMKSVGEVMSIGRTFKE
                     ALHKAIRSLEIDRFGLESAIRWSGGAPSEGERERVMDKLRVPNWERLWYVADALRLGL
                     TVEDVAAITRIDPWFLHQIAELVSAEEDLRARRARLIADGIRPDAENMLPRDVLAEAK
                     ALGFSDHALGRLVGSGEDAVRALREREGIRPVYKRVDTCGAEFVAHTPYLYSTYEPEC
                     ESQPTARRKVMILGGGPNRIGQGIEFDYCCVHASYALRELGYETIMVNCNPETVSTDY
                     DTSDRLYFEPLSLEDVLHIIRIERPLGVVVQFGGQTPLKLAVPLERAGVPILGTSPDA
                     IDRAEDRERFRELLVQLNLRQPESGTARTPGEALAIASRIGFPVMVRPSYVLGGRAMA
                     IVYDETSLDAYLAANLPANLATGVLIDQFIDDAVEIDVDAVSDGESVVVAGVMEHIEE
                     AGIHSGDSACSLPPYSLPADTLAEIERQTVQLAKALGVVGLMNVQFAVKGRDVYILEV
                     NPRASRTVPFVSKTLGVPLAKWAMKVMMGRKVADLGPAPRPLSYTAVKEAVFPFNRFP
                     GV"
     gene            complement(2753..3892)
                     /gene="carA"
                     /locus_tag="AC16BC_00660"
     CDS             complement(2753..3892)
                     /gene="carA"
                     /locus_tag="AC16BC_00660"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR3240149.1"
                     /translation="MTRAPKRPAILALADGTVFRGSAFGAEGESAGEVVFNTSLSGYQ
                     EILTDPSYRGQIVVMTAPQIGNYGVNPEDVESGRVWVEGFVVKECSPVSSNWRARQSL
                     DQYLKDAGIVGIQGIDTRALTKHLRDSGAQEGVLSTLDLDESRLVAKTKASPGLVGRD
                     LVRDVTCQKSYGWTEPIWSAASQASPLAGEGRVGGARVTVYDFGVKRNILRSLVSAGC
                     RVTVVPAGTPAADVLASRPDGVVLSNGPGDPAAVTYAVEAARNLVGRVPMFGICLGHQ
                     ILGLAMGGTTSKLKFGHHGGNQPVLDLATNQVTITSQNHGFTVDLDSLGGDVEATHVN
                     LNDRTVEGLRHKTLPIFSVQYHPEASPGPHDAGYLFQRFMDMVHR"
     gene            complement(3876..5177)
                     /locus_tag="AC16BC_00665"
     CDS             complement(3876..5177)
                     /locus_tag="AC16BC_00665"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3240150.1"
                     /translation="MTRQTDNRSILIRGGRVIDPASGLDAVCDLEITDGRITACGSGL
                     KPAAKTDSFNATGLVVCPGLIDLHTHVREPGYEYKETIKTATAAAAAGGFTTVFAMPN
                     TNPINDTRAVTELVLDVARRDGAVNVEPVGAITKGSKGEELAEIGELVAAGCAAISDD
                     GRPVTSSLIMRRALEYTLAFNVPVIDHCEDLCLSSGGVMHEGKVSTMLGLKGVPAAAE
                     TVMVARDLALAELTGGRLHLAHMSCAASVRMIREAKARGVRVTAEACPHHFSLTDEAV
                     IGFKADAKMNPPLRTREDVLAVIEGLADGTIDAIATDHAPHAPEEKSWPIAEAPFGVV
                     GLETALALSLALTADGHLSLPQVIALLTSKPAAVMGSDRGGLRVGAPADVTVFDPEAE
                     WVVDPAQFRSKSRNTPFAGWKMRGRVIRTIVGGKVVYDASA"
     gene            complement(5174..6124)
                     /locus_tag="AC16BC_00670"
     CDS             complement(5174..6124)
                     /locus_tag="AC16BC_00670"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR3240151.1"
                     /translation="MNLKRKDLIAIRDLDVEELRLLLDTAESFKEVAGREIKKVPTLR
                     GRTVVNLFFEPSTRTRSSFELAAKRLSADVINISSSSSSVVKGETLLDTAKNLEAMGA
                     DVIVVRHGESGAAALLARQLRCSVINAGDGAHEHPTQALLDLSTMLERKGRIEGLTVA
                     IVGDVRHSRVARSNLWGLTKLGARVRLAGPPTMLPAELKDFGAEIHYSLDTALEGADV
                     VMALRLQLERQSAGYFPSLREYSRFWGLTREQLQHAKPDVLVMHPGPVNRGVELAPDV
                     ADGLSSVILGQVANGVAVRMAALYLLTGGKSDSVAMAANQ"
     gene            complement(6121..6696)
                     /gene="pyrR"
                     /locus_tag="AC16BC_00675"
     CDS             complement(6121..6696)
                     /gene="pyrR"
                     /locus_tag="AC16BC_00675"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR3240152.1"
                     /translation="MPELNVSNGQERQVLDGSGVQRALSRMAHEIIERHKGVEGVVLV
                     GIRTNGVYLARRLAAKIAEIEGANVPLGELDITLYRDDLSTIKAHPLVRKTEIPFDLT
                     DRKVVLVDDVLYTGRTIRAAMDGVMDLGRPRFIQLAVLIDRGHRELPIRADYVGKNIP
                     TGPDEWVKVLLREEGQDDRVVILPGPERSAP"
     gene            complement(6883..7128)
                     /locus_tag="AC16BC_00680"
     CDS             complement(6883..7128)
                     /locus_tag="AC16BC_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3240153.1"
                     /translation="MVTLGAILVLLVAGPAGNAETGFGNPKRGKQIYEQLCARCHGMK
                     LDGHGPEAPSLRSAPADLQSLSSRTKSDWELLVIISR"
     gene            complement(7594..8622)
                     /locus_tag="AC16BC_00685"
     CDS             complement(7594..8622)
                     /locus_tag="AC16BC_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3240154.1"
                     /translation="MPKVLFKRSGQTISCEPGDYRLDLCESNNGAITFSCRAGACGAC
                     VIDVSATPDGLNPMSARERRTLEGVGADLARHRLACITRVMNDLEIAEATTAPSERAR
                     ETGVRPGFKAEVVESRHLADQVREVTFRLVQPASISFLPGQYLSFTVSDFDHVRRSYS
                     LASSPADRNHVTVCVRAVSGGRGSNYIHRLEPGDRVRFDGPYGDFVLRQSSARDILLI
                     ANGTGVAPMMSILRHLADAKSTRNVRLYFGLRHVHDLFYREELDQFAKHLPNFGYAYC
                     LSQPDLTRWDGCAGRVTDLLRKDLTRETALRSESYLCGGHGLVADAKAILLAAGMPAD
                     AIYHEQFY"
     gene            complement(8623..8931)
                     /locus_tag="AC16BC_00690"
     CDS             complement(8623..8931)
                     /locus_tag="AC16BC_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR3240155.1"
                     /translation="MSADESWVVYILRCRDGSLYAGITVDADRRLAQHQDGTASRYTR
                     SRRPVTMVYREPCASRSDALKRECAVKALTREAKERLISGSSRVDRRLPRRRGSSGRE
                     "
     gene            complement(8928..10478)
                     /locus_tag="AC16BC_00695"
     CDS             complement(8928..10478)
                     /locus_tag="AC16BC_00695"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3240156.1"
                     /translation="MNTGTDRDVDVEARPPMRERRASDRAALATVRAFAQQAFSRAAG
                     APLIEGNSVRLLKDARENYPAWLAAISAATRHVYFENYIISDDDTGRAFADALLAKAR
                     EGVRVRVLYDWLGCWGKTSGRFWKRLRAGGVEVRCYNPPRFDSPFGWINRDHRKLLAV
                     DGRVGFVSGLCVGRPWVGDPDRGMEPWRDTGVEVRGPAVADMVHAFADVWATTGAPLP
                     HGELASLDAISAAGDTALRVVASLPNTAGMLRLDQFVAALAKERLWLTDPYYAGTTPF
                     TQGLCTAVNDGVDVRLLVPGATDVPLVRPLSRAGYRPLLEGGVRVFEWNGPMMHAKTS
                     TADGRWARVGSTNLNIASWLGNCELDVVVEDERFAREMEEMYLDDLSRATELVLDERQ
                     RVRAPNQPRRRLMAARGGGSAGRAATGMLRLGNVVGASLTNRRVLEPVESRLMATTGA
                     LLLAVAMVFAVFPRVLAYPLIAVGAWLAVTLLVRGYRLRLTKKTGGPRDADDVGEPRP
                     SIDREGTE"
     gene            complement(10533..10820)
                     /locus_tag="AC16BC_00700"
     CDS             complement(10533..10820)
                     /locus_tag="AC16BC_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240157.1"
                     /translation="MSALILDHPEDPVVRLTIVDRDDDRIVFGITLESAARPDDVVQV
                     TEGLTIATEARSAARMEGVTLDYREPGGFRFIHPEPPPPQEESIFRMFDPG"
     gene            complement(10822..10971)
                     /locus_tag="AC16BC_00705"
     CDS             complement(10822..10971)
                     /locus_tag="AC16BC_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240158.1"
                     /translation="MAGGRYAGELRAAVSDAFVDGRAAPVDFSAFFCHHRSLFHGHRD
                     PSGSR"
     gene            11115..11321
                     /locus_tag="AC16BC_00710"
     CDS             11115..11321
                     /locus_tag="AC16BC_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240159.1"
                     /translation="MAKQSGCNLRTLLVGLVLLAILPAFALMLYTVAEQRRSTAANVQ
                     TDTLRLVKILSLNQARFSKAPANS"
     gene            11336..12043
                     /locus_tag="AC16BC_00715"
     CDS             11336..12043
                     /locus_tag="AC16BC_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HIR3240160.1"
                     /translation="MPAVVDRDPAACAAVLGPLLDRYPLYANLGAIETNGIIRCSALP
                     IHGPVNAADRSYFQRAMATREFAVGDYQIGRITNRASLNVGYPLLDPTGRVQTVVMAA
                     LDLGWLNRLVESASLPPGSVLTVVDRNGTVLLRQPDPGRWVGLSQAALPSIQTILREK
                     EGVTRERDADGVDRLVAFTTLGSAAADAGAYVSLGVPAAVAFEATDAAFRRSMIGLSA
                     AAVVALLAAWWGGAANS"
     gene            12019..13539
                     /locus_tag="AC16BC_00720"
     CDS             12019..13539
                     /locus_tag="AC16BC_00720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3240161.1"
                     /translation="MGGGGKLLILRRVTALMDAARRLQTGDLRARTGLPHADGELGHL
                     AHAFDNMAEALEAKTASLAFQAAHDALTNLPNRNRLVEHLRQAIRLGQRLGQPTSLLL
                     AGSHRFREITNTLGHRNGDRLLKEIGSRIQEMVRESDLVARGSGDTFAVLLPCTDLEG
                     AKLAADRILGRFKTPFLVNGMTVTIGIGFGIVLTPEHGDDPEILIRLADVALDQAKEG
                     GHSYFVYAPDHDRYEPDRLTLMGELPGAIEGDQLFLVYQPKIDLRRRTVVGVEALVRW
                     RHPQRGVVPPDQFILLAEHAGLIKPLTFWVLRAALEQAGAWRKDGLSLTVAANLSARV
                     LQDPKLPDQIADQIAAARGDPAWLELEITESVIMANPERARASLQALHAMGIGLSIDD
                     FGTGYSSLGYLKKLPVDTLKIDKSFVTDMTSDPDSEVIIRSTVELAHTLGLTVIAEGV
                     EDQEAWDRLASFGCDAVQGYYISEPMPSGDVTRWVNELWPARRLSAPGHAAERPAA"
     gene            13638..>14258
                     /locus_tag="AC16BC_00725"
     CDS             13638..>14258
                     /locus_tag="AC16BC_00725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR3240162.1"
                     /translation="MDQTLRVLLIEDSPDDALLLLRELKRAPYQTKHDRVDTADALKM
                     ALDRGPWDLILSDYSMPQFNGVAALRLVRGRGLDLPFIFVSGTIGEDTAVEAMRAGAN
                     DYIMKGNLKRLLPAVERELREANSRRSRRLAEAMVQRMAYYDALTQLPNRNKLFERFH
                     EVLRTDTRHDKSLALLLLDLNGFKEINDTLGHYRGDQVLQQVGARLR"
BASE COUNT         2420 a         4998 c         4439 g         2401 t
ORIGIN      
        1 ccacgcccgg aaaacggttg aacgggaaca ccgcttcctt gaccgcggtg tacgacagcg
       61 gccgcggggc cggacccagg tccgccactt tccgccccat catgaccttc atcgcccact
      121 tggccaacgg cacgcccaag gttttgctca cgaacggcac ggtgcgcgaa gcccgcgggt
      181 tcacttcgag gatatacacg tcgcggcctt tgaccgcaaa ctgcacgttc atcagcccga
      241 ccacgcccag cgccttggcc agttggacgg tctggcgctc gatctcggcc agggtatcgg
      301 cgggcaggga gtatgggggc aatgaacacg cgctgtcgcc ggaatggatc ccggcctcct
      361 cgatgtgttc catcaccccg gccaccacca ccgactcgcc gtccgacacc gcgtccacgt
      421 cgatttccac cgcgtcgtcg atgaactggt cgatcaacac gccggtggcc aggttcgcgg
      481 gcaggttcgc cgcaaggtag gcgtcgaggc tggtctcgtc gtacacgatg gccatggcgc
      541 gcccgccgag cacgtacgac ggccgcacca tcaccgggaa tccgatccgc gacgcgatcg
      601 ccaatgcctc ccccggtgtc cgcgcggtgc cgctctccgg ctgccgcagg ttgagctgca
      661 ccagcaattc gcggaaccgc tcccggtcct ccgcgcggtc gatggcgtcg ggcgacgtgc
      721 cgaggatggg caccccggcc cgctccagcg gcaccgccaa tttcaacggc gtctgccccc
      781 cgaactgcac caccaccccc agcggacgtt cgatccgtat gatgtgcaac acgtcttcga
      841 gcgagagcgg ctcgaagtag agacgatcgg acgtgtcgta gtccgtgctg accgtctcgg
      901 ggttgcagtt gaccatgatg gtttcgtagc ccagctcgcg gagcgcgtac gacgcgtgca
      961 cgcagcagta gtcgaattca atgccctgcc cgatccggtt cggcccgccg cccaggatca
     1021 tcaccttccg gcgggcggtg ggttgggatt cgcactccgg ctcgtacgtg gagtacaagt
     1081 acggcgtgtg cgcgacgaac tccgccccgc acgtgtccac gcgcttgtac accggtcgga
     1141 tgccttcccg ctcgcgcagc gcgcgcaccg cgtcctcacc ggatccgacc agccggccga
     1201 gcgcgtgatc ggagaacccc agcgccttgg cctcggccag cacgtcgcgc ggcaacatgt
     1261 tctccgcgtc gggtctgatc ccatcggcga tcaatcgggc gcgtcgcgcg cgcaggtcct
     1321 cctccgcgct cacgagttcc gcgatctgat ggagaaacca cgggtcgatc cgggtgatcg
     1381 cggccacgtc ctccacggtc agcccgaggc gcaaggcgtc cgcgacgtac cacagtcgtt
     1441 cccagttggg aacccggagc ttgtccatca cgcgttcccg ttcgccctcg gacggcgcgc
     1501 cccccgacca ccggatcgcg gactccaggc cgaagcggtc gatctccagc gagcggatcg
     1561 ccttgtgcag ggcttctttg aacgtgcgcc cgatggacat cacctccccg accgatttca
     1621 tctgggtggt cagcgtggcg tcggcttgag gaaacttctc gaacgcgaag cgcggaattt
     1681 tcacgaccac gtagtcgatg gtgggctcga acgcggcgcg ggtctggcgc gtgatgtcgt
     1741 tgggcagctc gtcgagcgta taccccaccg cgagtttggc cgcgatcttg gcgatcggga
     1801 acccggtggc cttggacgcc agcgcggagc tgcgcgacac ccgcgggttc atctcgatca
     1861 cggccaggtc cccgttctcc gggttcaccg cgaactggat gttcgacccg ccggtgtcca
     1921 ccccgatttc ccggatgatg gcgatcgcgg cgtcgcgcat cacctggtat tccttgtcgg
     1981 tcaaggtcat cgcgggcgcc acggtgatgc tgtcgccggt gtgcacgccc atggggtcca
     2041 cgttctcaat cgagcagatg atcaccacgt tgtcgcgcgt gtcccgcatt acttccagct
     2101 cgaattcctt ccaccccagc acggactgct ccaccaggat ctggtgggtc gggctggcat
     2161 ccagtcccca ctccaccgcg cgcttgaact cctcgcggtt gtaggcgacc gagccgcccg
     2221 cgccgcccag ggtgaacgac ggccggatga tcgcggggaa ccccacccgc tcgatcacgg
     2281 ccatcgcgtc ttcaaccgac gacacggtgc cgctctccgg gaccttgagg ccgacgcggc
     2341 ggacggcttg tttgaagcgt tcgcgatcct cggccttttt gatcgcgtcg tactgcgcgc
     2401 cgatcagttt gacttggtac ttttccagca cgccccgttc caccaacgcc accgccgtgt
     2461 tcagcgcggt ctgcccgccc atggtgggca gcagcgcgtc gggccgctcg cgctcgatga
     2521 tgcgctcgac gacctcgggc gtaatcggct cgatgtaggt gcggtgcgcc aactcgggat
     2581 cggtcatgat ggtggccgga ttcgaattga ccagcaccac ctcgtaccct tcctcacgca
     2641 acgccttgca cgcctgcgag cccgagtaat cgaactcgca cgcctggccg atgacgatcg
     2701 ggccggagcc gatcaacagg atcttccgga tgtcggtgcg ctttggcatt ccttacctgt
     2761 gaaccatgtc catgaagcgt tggaacaaat accccgcgtc atgcggaccg ggtgaggctt
     2821 ccgggtggta ctgcaccgaa aagatcggca aggtcttgtg ccgcaagcct tccacggtcc
     2881 gatcgttcag attcacgtgc gtggcttcga cgtccccgcc caatgaatcc agatccacgg
     2941 tgaagccgtg gttctgggac gtgatcgtca cctggttcgt ggccagatcc agcaccggct
     3001 ggttgccccc gtggtggccg aacttcaact tggaagtcgt gccgcccatg gccaggccca
     3061 ggatctggtg cccgaggcag atgccgaaca tgggcacgcg cccgaccaag ttccgcgcgg
     3121 cttccaccgc gtaggtcacg gccgcgggat cacccggacc attggacagc accacgccgt
     3181 ccggccgcga ggccaacacg tcggcagcgg gcgtgcccgc gggaaccacg gtgacccgac
     3241 acccggccga gaccaggctg cggagaatgt tgcgcttcac accgaagtca tacacggtca
     3301 cgcgcgcccc ccccacccga ccctcccccg caagggggga ggcctgagac gcggcgctcc
     3361 atatcggttc tgtccaccca tacgacttct gacacgtgac gtcacggacc aggtcacggc
     3421 ccacgagtcc cggcgacgcc ttcgttttgg ccaccaagcg tgactcatcc aagtcgagcg
     3481 tcgacagcac gccttcctgc gcgccggaat cccgaaggtg tttggtcaac gcgcgggtgt
     3541 cgattccttg gatgcctaca atgccggcat cctttagata ttggtcgaga ctctggcgcg
     3601 cgcgccaatt gctcgacacc ggcgagcact ccttgaccac aaaaccttcg acccacaccc
     3661 ggccggactc cacgtcttcc gggttgactc catagttgcc gatttgcggg gccgtcatga
     3721 ccacgatctg gccgcggtac gacgggtccg tgaggatctc ctgatagccg gacagactgg
     3781 tgttgaacac cacttcgccc gcggactcgc cctccgcgcc gaacgcggag ccgcgaaaca
     3841 cggttccgtc cgccaacgcg aggatcgcgg gccgcttagg cgctcgcgtc atataccacc
     3901 ttcccaccga cgatcgtcct gatcacgcgg ccgcgcatct tccatcccgc gaacggcgtg
     3961 ttccggctct tcgaacgaaa ctgcgccggg tccaccaccc attcggcttc ggggtcgaag
     4021 acggtcacgt ccgcgggcgc gcccacccgc aaaccgcctc gatcactgcc catgaccgcg
     4081 gcaggtttgg atgtcagcag cgcgatcacc tgcggcaacg acaggtgccc gtccgcggtc
     4141 agcgccagtg acagcgccag cgcggtctcc aacccgacca ccccgaacgg cgcctccgcg
     4201 atgggccacg acttttcttc cggcgcgtga ggcgcgtgat ccgtggcgat cgcgtcgatg
     4261 gtgccgtccg ccaagccctc gatcaccgcg agcacgtcct cccgggtccg caggggcggg
     4321 ttcatcttgg cgtcggcctt gaatccgatc acggcttcgt ccgtcaacga gaagtgatgc
     4381 gggcaggcct ccgcggtcac gcgcacgccg cgggccttgg cctcccggat catgcgcacc
     4441 gacgcggcgc agctcatgtg cgcgaggtgc agccgcccgc cggtcagctc ggccaaggcc
     4501 aggtcgcgcg ccaccatcac ggtttcggcc gcggcgggca cgcccttgag tcccagcatg
     4561 gtcgagacct taccctcgtg catcaccccg ccggaggaca agcagaggtc ttcgcagtgg
     4621 tcgatcaccg gcacgttgaa cgcgagcgtg tattccaagg cgcgccgcat gatcagactg
     4681 ctggtgacgg gccgcccgtc gtccgagatc gcggcgcagc ccgccgccac cagttcgccg
     4741 atctcggcca gctcttcgcc cttcgacccc ttggtgatcg cgcccacggg ttcgacgttc
     4801 accgcgccgt cgcgacgggc cacgtccaac accagttcgg tgaccgcgcg cgtgtcgttg
     4861 atgggattgg tgttgggcat ggcaaagacc gtggtgaagc ctccggccgc ggccgctgcg
     4921 gtggcggtct tgatcgtctc cttgtattcg tacccgggtt cgcgcacgtg cgtgtgcaga
     4981 tcgatcagtc ccgggcagac cacgagcccg gtcgcgttga acgagtcggt cttggccgcg
     5041 ggcttgaggc ccgagccgca ggccgtgatc ctgccgtcgg tgatctccag atcacacact
     5101 gcgtcaaggc cgctcgccgg gtcgatgacc cgcccgcccc ggatcaggat ggagcgattg
     5161 tcggtctggc gcgtcattgg ttggccgcca tggccacgga gtccgatttg ccgccggtca
     5221 gcaggtagag cgcggccatg cgcaccgcga ccccgttggc tacctgtccc aggatcacgg
     5281 acgagaggcc gtccgccacg tccggcgcca gctccacccc gcggttgacc gggccgggat
     5341 gcatgaccag gacatcgggc ttcgcgtgct ggagctgctc ccgagtcagg ccccagaacc
     5401 ggctatactc ccgcagcgac gggaagtacc ccgcgctttg acgctccagt tgcagccgca
     5461 acgccatcac cacgtccgcg ccctccaacg ccgtatcgag cgaataatga atctccgcgc
     5521 cgaaatcctt gagttccgcc ggcagcatgg tcggcggtcc cgccagccgc acgcgcgcgc
     5581 ccagcttggt caggccccac agattggagc gcgccacccg gctgtgccgg acgtcgccca
     5641 cgatcgccac cgtgagcccc tcgatccgcc ccttgcgttc cagcatggtc gagagatcca
     5701 gcagcgcctg cgtgggatgt tcgtgcgcgc cgtcccccgc gttgatcacg gagcagcgca
     5761 actgccgcgc gagcaacgcg gccgcgccgg actcgccgtg gcggaccacg atcacgtccg
     5821 cgcccatcgc ctccagattc ttggccgtgt ccagcagggt ctcgcccttg accacgctgc
     5881 tggaggagga cgagatgttg atcacgtccg cggacaagcg tttggccgcc agctcgaacg
     5941 acgaccgcgt gcgcgtactg ggctcgaaaa acaggttgac cacggtgcgg ccccgcaggg
     6001 tcggcacctt tttgatctca cgcccggcca cctccttgaa cgactccgcg gtgtcgagca
     6061 acagccgcaa ttcctccacg tccagatccc ggatcgcaat caggtccttg cgtttgagat
     6121 tcatggcgcg ctcctctcgg gcccgggcag aatcaccacc cggtcgtcct gcccttcttc
     6181 gcgcagcaac accttgaccc attcgtcggg accggtggga atgttcttcc ccacgtaatc
     6241 ggcccggatc ggcaactcgc ggtgtccgcg gtcgatcaac acggccagct ggatgaaccg
     6301 gggccggccc agatccatca ccccgtccat cgcggcgcgg atggtgcggc cggtgtagag
     6361 cacgtcgtcg accagcacca ccttgcgatc ggtcagatcg aacggaatct cggtcttgcg
     6421 cacgagcgga tgcgccttga tggtcgagag gtcgtcccga tagagcgtga tgtcgagttc
     6481 gcccaacggc acgttcgcgc cttcgatctc cgcgatcttt gcggccagtc ggcgggccag
     6541 atacacgccg ttggtcctga tcccgaccag caccacgccc tcgaccccct tgtggcgctc
     6601 gatgatctcg tgcgccatgc gcgagagcgc gcgctggaca ccggaaccgt ccaggacctg
     6661 gcgctcctgt ccgttggaca cgttcagctc gggcataaaa aagccttcca agccgcaggg
     6721 gcgtggaagg tctcgtgttg gctggaaacg tcgttcatgg cttcctttct agcctcgcag
     6781 ggctagccat taaaggcaca cggactctac gcgatcggac cgcgcgctgt caaggacgag
     6841 tgcaccgcgg caaaccggtt gagccggacg ctgaacaacg cgtcaccggc tgatgatgac
     6901 cagcagttcc cagtcgctct tggtgcgcga actcagcgac tgcaaatcag ccggcgcgct
     6961 ccgcagcgag ggcgcttcgg gtccgtgacc atcaagcttc atgccgtggc agcgcgcgca
     7021 caattgctcg tagatctgtt tgccgcgctt gggattccca aacccggtct ccgcgttgcc
     7081 ggccggaccc gcgaccagga gcaccaggat cgcgccgaga gtcaccattg ccacacgtgt
     7141 cattgcaccg agagccgaac gaccgtccat tgcgtcacct cgcaacgaat gagtgtccgt
     7201 gagcccttgc ccaccacgca acgtgaacct ttcctgccct tcccctcaca ccagctgatg
     7261 gtgagagact gattgaacag tatggacaac tccactcgcc tccaccatag cccgcaagga
     7321 ctaggaggac cctgcaaaac tcagtgatac tttcaggctg ctcaaaaagc tccggatgcc
     7381 cccttcgggg acttccggga tttgagttgt tggtggtggg tcgcaaggcc tcggaggagg
     7441 aaaccggagc gtactgtgtc gtacgtgagg atttccgacg accgagaagc tgtgccgcac
     7501 cgtcggtgcg gaatgaaatc ggttgctctt ttcctcacag atcgcgaagc aacgccgcag
     7561 ccggactttt tcagcagccg cctacgcccg cggtcagtag aactgctcgt ggtagatcgc
     7621 gtccgcgggc attcccgcgg cgaggagaat ggccttggcg tccgccacca ggccgtgccc
     7681 gccgcagagg taggattccg agcgcaacgc cgtctcgcgc gtgaggtcct tgcgcagcag
     7741 atccgtgacc cggcccgcgc acccgtccca tcgggtgaga tccggttgcg acaagcagta
     7801 cgcgtaacca aagttcggga ggtgtttcgc gaactggtcc aattcttccc ggtagaacag
     7861 atcgtgaacg tgccggagcc cgaaatacag ccggacgttc cgcgtggact tggcatccgc
     7921 caggtggcgg aggatcgaca tcatgggcgc caccccggtg ccgttggcaa tcaacagaat
     7981 atcccgcgcc gatgattggc gaaggacaaa gtccccgtac ggaccgtcga accggacgcg
     8041 gtcgcccggc tccagccggt ggatgtaatt ggacccgcgg cccccgctca cggcccgcac
     8101 gcacacggtc acgtgattgc ggtcggccgg agacgacgcc aggctgtacg accggcgcac
     8161 gtggtcgaaa tccgacaccg tgaacgagag gtattgtccg gggagaaacg agatgctcgc
     8221 gggttgaacg agccgaaacg tgacctcccg cacctgatcg gccagatggc ggctctccac
     8281 tacttcggcc ttgaacccgg gacggacgcc ggtttcgcgg gcgcgctcgg agggcgcggt
     8341 cgtggcctcc gcgatttcca gatcattcat cacgcgggtg atacaggcca accggtgtct
     8401 ggccagatcc gcgcccacgc cctccagcgt gcgccgctcc cgggcggaca tgggattcaa
     8461 accatcgggc gtagccgata cgtcgatcac gcacgcgccg cacgcgcccg cccggcagct
     8521 gaacgtgatc gcgccgttgt tcgactcgca gaggtccaac cggtagtccc cgggctcgca
     8581 cgagatcgtt tgcccggacc gtttgaacag caccttcggc atctactccc tccctgaaga
     8641 cccgcgacgg cgcgggagcc ggcgatccac gcgcgacgag ccgctgatca atcgctcctt
     8701 ggcctcacgc gtcaaggcct tgaccgcgca ctcgcgcttc aaggcgtcgg atcgactcgc
     8761 gcacggctcc cgatagacca tcgtcaccgg cctgcggcta cgagtatacc gggacgcggt
     8821 gccgtcctgg tgttgcgcca gccgacgatc agcgtccacc gtgatcccgg cgtacaacga
     8881 cccgtcgcgg caccgcagga tatacaccac ccacgattcg tccgcactca ctccgtcccc
     8941 tcgcgatcga tcgagggacg cggctcgccg acatcgtcgg catctcgcgg tccacccgtt
     9001 tttttcgtga gccggagtcg ataccccctc accagcagcg tgacggccag ccaggcgccc
     9061 accgcgatca gcggatacgc caacacgcgc gggaagacgg cgaacaccat ggccacggcc
     9121 agcagcaatg cgccggtcgt ggccatgagg cgggactcga cgggttcgag cacgcgacgg
     9181 ttcgtgagcg acgcgcccac cacgttgccg aggcgcaaca tcccggtggc ggcgcggccc
     9241 gcgctcccgc ccccccgtgc ggccatgagg cgccgccggg gctgattcgg cgcgcggacc
     9301 cgctgccgtt cgtccaggac gagttcggtg gcgcgcgaca aatcgtccag atacatctcc
     9361 tccatctcac gcgcgaaccg ctcgtcctcg accaccacgt ccaactcgca attgcccagc
     9421 caactggcga tgttcaggtt cgtggacccc acgcgcgccc accgcccgtc cgcggtcgag
     9481 gtcttggcgt gcatcatggg accgttccac tcgaacaccc gcacgccgcc ttcgagcagc
     9541 ggacgatagc cggctcgcga caagggtcgc acgagcggga cgtcggtcgc gcccggcacc
     9601 agcaaccgca cgtccacgcc gtcattcacc gcggtgcaca aaccctgcgt gaagggcgtg
     9661 gtcccggcgt agtaaggatc ggtcaaccac aggcgttcct tcgcgagcgc ggccacgaat
     9721 tgatccaggc ggagcatgcc ggccgtattc ggcagactcg ccacgacgcg cagcgcggtg
     9781 tcgcccgcgg ccgagatggc atcgaggctc gccagttcgc catggggcag gggcgcgccc
     9841 gtcgtggccc agacgtcggc gaatgcgtgg accatgtccg ccaccgccgg accgcgcact
     9901 tcgaccccgg tgtcgcgcca cggttccatc cctcgatccg gatcgcccac ccacggccgc
     9961 cccacgcaca gccccgagac gaacccgacc cgcccgtcga ccgcgagcag cttgcggtga
    10021 tcgcgattga tccacccgaa gggcgaatcg aagcgcggcg ggttgtaaca gcgcacctcg
    10081 acgccgcccg cgcgcaggcg cttccagaac cgccccgagg tcttccccca gcaccccagc
    10141 caatcgtaga gcacgcgcac ccgcacgccc tcgcgcgcct tcgccagcaa cgcgtccgcg
    10201 aaggcgcggc ccgtgtcgtc gtcgctgatg atgtagttct cgaaatacac gtggcgagtc
    10261 gccgcggaaa tggccgcgag ccacgccggg taattctcgc gcgcgtcctt gagcaggcgg
    10321 acgctgttgc cctcgatgag cggcgcgccc gccgcgcgcg aaaacgcctg ctgcgcgaag
    10381 gcgcgcacgg tggcgagcgc ggcccggtcg gaggcgcggc gctcccgcat cggcgggcgc
    10441 gcctccacgt caacgtcgcg gtcagttccc gtattcatcg tgatcaccgc gggccattaa
    10501 gcctctcggc gcgttcgccg gccgcacggc gcctagcccg gatcgaacat ccggaaaatc
    10561 gattcctctt gagggggcgg cggttcggga tggatgaacc ggaacccgcc cggttcgcgg
    10621 taatccagcg tcaccccctc catgcgcgcc gcgctgcggg cttcggtcgc gatggtcaag
    10681 ccctcggtca cttgcaccac gtcgtccggc cgcgccgcgg attccaacgt gatcccgaag
    10741 acaatgcgat catcgtcccg gtcgacgatg gtcaagcgca ccaccgggtc ttcgggatga
    10801 tcgaggatca gtgcgctcaa tttagcgcga gccggagggg tcacgatgac catggaacag
    10861 gctgcgatgg tgacagaaga acgcggaaaa atccactgga gccgcgcgtc cgtcgacgaa
    10921 cgcgtccgag acggccgcgc ggagttcgcc cgcgtaccgg cccccggcca tgactgcgct
    10981 tcgtgcacac gggtcgcgct cgtgccgggc tcttgagaag cactcggcgt tgacaggccg
    11041 tggtgaactg ataccctggg ggcgcgcttc tccgatgggt tcccatcaat cgccacggac
    11101 ggcaggagaa ccgtatggcg aaacaatcag gatgcaatct gcggacgctg ctggtgggcc
    11161 tggtcctatt ggccatcctg cccgccttcg cgctcatgct ctatacggtc gccgaacagc
    11221 gtcgatccac cgccgccaac gtccagaccg atacgctccg gctggtcaaa atcctctccc
    11281 tgaatcaggc tcgattctcg aaggcgcccg ccaattcctg atcgccctct cgcaagtgcc
    11341 tgcggtcgtc gaccgtgatc ccgccgcatg tgcggcggtg ctgggtccgt tgctcgaccg
    11401 gtatcccctg tacgcgaacc tcggcgccat cgaaacgaac gggatcattc gctgcagcgc
    11461 gttgccgatc catggcccgg tcaacgccgc cgaccggtcg tactttcaac gcgcgatggc
    11521 cacacgcgag ttcgccgtgg gcgactatca gatcggccgc atcacgaacc gggcgagcct
    11581 caacgtgggg tatccgctcc tcgatccgac cgggcgcgtc cagaccgtcg tcatggccgc
    11641 gttggacctc ggctggctca atcggctggt cgagagtgcg tcgctgcctc ccgggtcggt
    11701 gttgacggtc gtggatcgga acggcacggt gctcctgcgt caacccgacc ccggccggtg
    11761 ggtcggactg tcgcaggccg cgctgccttc catccaaacc atcctccgcg agaaggaggg
    11821 cgtcacgcgc gagcgggatg cggacggcgt ggaccgcctc gtggcgttta ccaccctcgg
    11881 ctccgcggcg gccgacgcgg gcgcgtacgt cagtctcggt gtccccgccg ccgtggcgtt
    11941 cgaggccacg gacgccgcgt tccgacgcag catgatcgga ctcagcgcgg ccgccgtggt
    12001 cgcgttgctc gcggcctggt gggggggggc ggcaaactcc tgatccttcg gcgcgtcacg
    12061 gcgctcatgg acgccgcgcg ccggctccag acgggagatc ttcgcgcgcg gaccggactg
    12121 ccccacgcgg acggcgagtt gggtcacctc gcgcatgcgt tcgacaacat ggccgaggcg
    12181 ctggaggcaa aaaccgcgtc actcgccttt caggcggcac acgacgccct cacgaatctg
    12241 ccgaaccgca atcgcctggt ggaacacctc cgtcaggcga ttcggctggg acaacgcctg
    12301 gggcaaccca cctcccttct gctggcgggg tcccatcgct tcagggagat caccaacacg
    12361 ctggggcatc gcaacggcga ccggctcctc aaagagatcg gatcgcggat ccaggaaatg
    12421 gtgcgggaat ccgatctggt ggctcggggc agcggcgata ccttcgccgt gctcctgccg
    12481 tgcaccgacc tggagggcgc caagctggcc gcggaccgga ttctcggtcg attcaaaacc
    12541 ccgtttctgg tcaacggcat gaccgtcacg atcgggatcg gcttcggcat cgtgttgacc
    12601 ccggaacacg gcgacgatcc ggaaatcctg attcgcctcg cggacgtggc gctcgaccaa
    12661 gccaaggaag gcggtcacag ctatttcgtc tacgcccccg accacgaccg ttacgaaccg
    12721 gaccgactga ccctcatggg agagttgccc ggcgcgatcg aaggcgacca actgttcctg
    12781 gtctatcaac ccaagatcga cctccgtcga cgaaccgtgg tgggcgtgga agcgctcgtg
    12841 cgctggcgcc atccccaacg cggcgtggtt ccaccggatc aattcatcct gctggcggaa
    12901 cacgcggggt tgatcaaacc gctcacgttc tgggtcctgc gggccgcgct cgaacaagcg
    12961 ggtgcgtggc ggaaagacgg gctctcgctg acggtggccg ccaatctgtc ggcgcgagtg
    13021 ctccaggacc cgaagctgcc ggaccagatc gcggaccaga tcgccgccgc ccggggcgat
    13081 cccgcctggc tggaactcga gatcaccgaa agcgtgatca tggccaatcc cgagcgcgcg
    13141 cgcgcgagtt tgcaggcgct ccacgccatg ggcatcgggc tttcgatcga cgacttcgga
    13201 accggctatt cgtccctcgg atatctcaag aaactcccgg tggacacctt gaaaatcgac
    13261 aagtcgttcg tgacggacat gacgagcgat ccggacagtg aagtgatcat ccgctccacc
    13321 gttgaactcg cccatacctt gggcctcacc gtgatcgcgg agggcgtgga ggaccaggaa
    13381 gcgtgggacc ggctcgcgtc gttcgggtgc gacgcggtcc aaggctacta catcagcgag
    13441 cccatgccgt cgggcgacgt cacccgctgg gtcaacgaac tgtggccggc ccgccgattg
    13501 tcggcgcctg gacacgccgc ggaacgaccg gcggcgtgac ggcccccctt caagccgcat
    13561 tcatgttcaa ccccacccat ccttgacggc aaccggcgtc atgatacagt ctgctgcgtc
    13621 ccaatggagg ggatgatatg gatcagaccc tgcgcgttct cctcatcgag gactctcctg
    13681 acgatgcctt gctgctcctt agagagctaa aacgcgcccc ctatcagacc aagcatgacc
    13741 gcgtggacac cgccgacgcg ctcaagatgg cgctggatcg cgggccatgg gatctcatct
    13801 tatcggacta ctcgatgcct cagttcaatg gcgtcgccgc cctgcgcctc gtcagggggc
    13861 gagggctcga ccttcccttt attttcgtgt ccgggaccat cggggaagac acggcggtcg
    13921 aggcgatgag ggccggggcg aacgactaca ttatgaaggg caatctcaag cgactgctcc
    13981 cggccgtgga gcgggaattg cgcgaggcca atagccggcg ctcccgccgc ctcgctgaag
    14041 ccatggtgca gcgcatggcc tattatgacg ccctgaccca actcccgaat cgcaacaaat
    14101 tgttcgagcg cttccatgaa gtccttcgaa ccgacacccg tcacgacaag tccctggcct
    14161 tgctgctcct ggacttaaac ggcttcaagg agatcaacga taccctgggc cactatcggg
    14221 gcgaccaggt gttgcaacag gtgggagcgc gcctgcgg
//
LOCUS       DBNGTT010000032         5345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2178192, whole genome shotgun sequence.
ACCESSION   DBNGTT010000032 DBNGTT010000000
VERSION     DBNGTT010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2178192"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1935
                     /locus_tag="AC16BC_00730"
     CDS             <1..1935
                     /locus_tag="AC16BC_00730"
                     /inference="COORDINATES: protein motif:HMM:NF022747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR3240163.1"
                     /translation="KPLGQRQTEDRGVYDLRFWGKNRLVMGQYDPVAKVGRKVSDYLL
                     SADLSEDRHTAATIQTGSSPARLVVDPSGEFVLTANVDDDEVTIVNEHGATARITLAP
                     GPRDILFADRGKRLIVICWKPGARESEVFTLSANFAERPWPLFKADKNAKVPGGLIDA
                     ALSPDGRVIYALDRLGKRLVSFDATTLEQVGGIAVGDEPTAFVLREVSSRERDRLAQK
                     TPARKRLEDIVATMKNRGKAFTDAAFTETMTREVQEEATGDAEKAGDGKADSAPTTKT
                     ATSTVKTSLRLPDSVRQEFEDGGLRLAQGGRAYSVTKAGRYHQTPRQDLVYLLYTVYG
                     QPTDAWIRALAGDVPGSPYLRNGIAVDIVRTVEEENHRYYAIGAMNAHDPVSQLWISA
                     ETGLPVSLVEQFPMMRSKNPHEQGEGFQGLTETRLHYQKLEGGYTIPTTLTRYLDGKE
                     LGRVEVSQTAFNQNPSSDRFDLARLGGAIKPLTVKAAPAKAAQKGPGIAVSGLGNEHV
                     ETPFGEHAAYNSNPPTSGPHTPYLADWGVHKAPIAPETQVHNLEDGGVLIQYSCPTAC
                     PDLAAKLEALAGKYERVVIAPYPLMTQRIALTAWERIDLLTDYDEARVTAFVDAYIGK
                     DHHPAGGEGAAQSEGGKDTK"
     gene            1932..2612
                     /locus_tag="AC16BC_00735"
     CDS             1932..2612
                     /locus_tag="AC16BC_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024823.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240164.1"
                     /translation="MNRVIGMAVAAALGAVAIQGCQSKPGTGLPEPVAEQSGASAPGG
                     QPSGALPSNHPPMSATGPIPQSPPAVDRPLPAGTRNPMEDIMAFKARLDKNPNDYEAL
                     VSLGNANMMISRLEAAQELYVRALAINPKDVDARTNLAIAYKYGGKPEQAFAELNKNL
                     AIDPDNDSTLYNLGFLYLYDKHDRKEALAIWKKWLALYPNAPAAADVSKQIAQIETEM
                     GKGAVSSK"
     gene            complement(2717..3433)
                     /locus_tag="AC16BC_00740"
     CDS             complement(2717..3433)
                     /locus_tag="AC16BC_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3240165.1"
                     /translation="MVLIPAGDFLMGSDRIDTEGKSSEFGFAKPLYLDEHPQQPITLP
                     DFRIDRYEVTQDAYAVFVRKTGAPAPDHWTNGAPPAGTARRPVTGVDWFQAQRYCEWR
                     GKRLPTEAEWEKAARGTDGREYPWGNEYDGQKANTGDAGRGGLVDVGSFPQGRSPFGV
                     EDMAGNAWEWTSDWYLPHPGSTYDADRFGRTFKVLKGGSWGGVGHYALSEFYRSAYRF
                     PQPPVARYRDFGFRCAQSAA"
     gene            complement(3577..4383)
                     /locus_tag="AC16BC_00745"
     CDS             complement(3577..4383)
                     /locus_tag="AC16BC_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3240166.1"
                     /translation="MTRAFVVALLAALAITVGCKGATGRDAASPPQIGAMVVIPQGDF
                     IMGSDQIDTAQQGSEFGTAKPLFLDEHPKRTVTLPAYQIDAYEVTNQQYRAYVDATRA
                     PAPPTWPDGRFAPEHATWPVTGVTWFEADAFCRWAGKRLPTEAEWEKAARGTDGREYP
                     WGNEFDTTKGNIGGTGLAPVGSFPQGVSPYGVYDMAGNVSEWVGDWYHAYPGSTYANP
                     AFGQKFRSVRNSSWGGSAGHYTVAHFYRAAYRFYHAPEQRFRDIGFRCAR"
     gene            complement(4380..>5345)
                     /locus_tag="AC16BC_00750"
     CDS             complement(4380..>5345)
                     /locus_tag="AC16BC_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR3240167.1"
                     /translation="DLRTEKQFDLYTPANTGGRILSNGIYTIEIDRQGDKWIGTYGGG
                     LYRFDGTTWTNYNPYGSGSTIDYQKSWTAYTPGRGLGDLWVYDVAFDRKGQMWVATWK
                     GVSRFDGAGFTTYTVDAGLVDKWVYAIAIDQQGVLWFGTEGGVSRFDGTNWKSYTHAD
                     GLGLDTGEVVKDPSYAFGNPHHLDPSKQAAATNPNYVVAAAIDRNDHKWFGTYGGGVS
                     RFDGRTWTTYSKADGLGGNFVFALTVDHNGLLWAGTDGGASWFDGNHWRTYTTRDGLI
                     DNFVYSIAVDEDGNKWFGTRVGVSRFSDPDRGWLKAFFNRFGLFL"
BASE COUNT          962 a         1708 c         1784 g          891 t
ORIGIN      
        1 aagcccctcg gccagcgcca gaccgaggac cgcggcgtgt acgacctgcg cttttggggc
       61 aagaaccggc tggtgatggg gcagtacgat cccgtcgcca aggtcgggcg caaggtgtcg
      121 gactacctgt tgagcgcgga tctgtcggag gatcgccaca ccgcggcgac cattcagacc
      181 gggtcgagtc ccgcgaggtt ggtcgttgat ccgagcgggg agttcgtgct gaccgcgaac
      241 gtggacgacg acgaggtcac gatcgtgaac gaacacggcg cgaccgcgcg gatcacgctg
      301 gcgccggggc cccgcgacat cctgtttgcg gaccgcggca agcgcctgat cgtgatctgt
      361 tggaagccgg gggcgcggga gagcgaagtg ttcacgctga gtgcgaactt tgccgagcgg
      421 ccgtggcccc tgtttaaggc cgataagaac gcgaaggtgc cgggcggcct gatcgatgcg
      481 gccctgagtc cggacggccg cgtcatctac gcgttggatc gcctgggcaa gcgcctggtc
      541 tcgttcgacg cgaccacgct ggagcaagtc ggcggcatcg cggtggggga cgagcccacc
      601 gcgttcgtgc tccgcgaggt gtcatcccgc gagcgggatc ggctcgcgca aaaaacgccg
      661 gcccgcaaac gcctggaaga catcgtggcc accatgaaga accgcggcaa agcgtttacc
      721 gacgcggcgt tcaccgaaac catgacccgt gaagtccagg aggaggccac gggtgacgcc
      781 gaaaaggcgg gcgacggcaa ggccgattcc gcgccgacga ccaagaccgc gacgtcgacc
      841 gtgaaaacgt cgttgcggtt gccggactcg gtgcgccagg agttcgagga cggcgggctg
      901 cggctggccc agggcggccg cgcgtactcc gtgaccaagg ccgggcggta ccaccagacg
      961 ccgcggcagg acctcgtcta tctgctgtac acggtgtacg ggcagccgac cgacgcgtgg
     1021 atccgggccc tggccggcga cgtgccgggc agcccgtatc tgcgtaacgg gattgcggtc
     1081 gatatcgtgc gcaccgtgga agaggagaac caccggtact acgcgatcgg cgccatgaac
     1141 gcgcacgacc ccgtgtccca gttgtggatc agcgcggaaa ccgggttgcc cgtgagcctg
     1201 gtggagcagt tcccgatgat gcggtcgaag aatccgcacg agcagggtga aggattccag
     1261 ggcctgaccg agaccagact ccactatcaa aaactcgagg gcggctatac cattccgacc
     1321 acgctcaccc ggtatcttga cggcaaggag ttgggacgcg tcgaggtctc gcagaccgcg
     1381 ttcaaccaaa acccgtcctc ggatcggttc gacctggcgc ggttgggcgg tgcgatcaag
     1441 ccgttgacgg tcaaagccgc gccggccaag gccgcgcaga aggggccggg gattgcggtg
     1501 tcggggttgg gcaacgagca cgtggaaacg ccgttcgggg agcacgccgc gtacaacagc
     1561 aacccgccca catccgggcc ccataccccg tatctggcgg actggggcgt gcacaaggcg
     1621 ccgatcgcgc cggagacgca ggtccacaac ctggaagacg gcggggtgct gattcagtat
     1681 tcgtgtccca cggcgtgtcc ggaccttgcg gccaagctcg aagccttggc gggcaagtac
     1741 gagcgagtcg tgatcgcccc gtacccgttg atgacgcagc gcatcgcgct gaccgcgtgg
     1801 gagcggatcg acctgctgac cgactacgat gaggcgcggg tgaccgcgtt cgtcgacgcc
     1861 tacattggga aagaccacca cccggccggc ggggaagggg ccgcgcagtc cgagggggga
     1921 aaggacacga aatgaaccgc gtgatcggga tggcggtcgc ggcggcgctg ggcgcggtcg
     1981 cgatccaggg gtgccagtcc aaacccggaa ccggccttcc ggagccggtg gcggaacaga
     2041 gcggcgcgtc cgcgccgggc ggccaaccgt ccggcgcgct gccgtccaat catccgccca
     2101 tgtcggccac gggcccgatt cctcagtcgc cgccggcggt cgaccgcccg cttcccgcgg
     2161 gcacgcgcaa ccccatggaa gacatcatgg cgttcaaggc gcggctggac aagaacccga
     2221 atgactacga agccttggtg tccctgggca atgccaacat gatgatctcc cggttggagg
     2281 cggcgcaaga gctgtacgtg cgcgcgctgg ccatcaaccc gaaggatgtc gacgcgcgga
     2341 ccaacctggc cattgcctat aagtatgggg gcaagcctga acaggcgttc gcggaactga
     2401 acaaaaattt agcgattgat cccgacaacg attccacgtt gtataacctc ggcttcttgt
     2461 acttgtacga caaacacgac cggaaggaag cgctggccat ttggaagaaa tggctggcgc
     2521 tctacccgaa cgcgccggcg gctgcagacg tgagcaagca gatcgcgcag atcgaaacgg
     2581 aaatggggaa gggcgccgtg tcgtcgaagt aggagcacgg cagccgtgcc cctacgtggg
     2641 attgttcggt gtgtctggga gtgcggcgat acgccggccg ttggtggttc gggggcgcac
     2701 ggccgtgcgc ccgacgctat gccgccgact gcgcgcagcg gaacccgaag tcgcgatagc
     2761 gcgcgaccgg cggctgggga aaccgatacg cgctccggta gaactccgac agtgcgtagt
     2821 gtcccacacc gccccagctc ccgcccttca acactttgaa ggtgcgcccg aaacggtccg
     2881 cgtcgtacgt gctgccgggg tggggcaagt accaatccga cgtccattcc cacgcgttcc
     2941 cggccatgtc ctcgacccca aacgggctgc ggccttgggg gaatgagccc acgtccacga
     3001 gcccgccgcg ccccgcgtcg cccgtattgg ctttctgtcc gtcgtactcg ttgccccagg
     3061 ggtattcgcg accgtcggtg ccgcgcgcgg ccttctccca ctccgcttcg gtgggcaacc
     3121 gcttgccgcg ccactcgcag tagcgctgcg cctggaacca gtccacgccc gtgaccggcc
     3181 gtcgcgcggt tcccgccggg ggggcgccgt tggtccagtg gtcgggcgcg ggcgcgccgg
     3241 tcttgcgcac gaacacggca tacgcgtctt gggtcacctc ataccgatcg atacggaagt
     3301 ccggcagcgt gatgggttgc tgcgggtgct cgtcgaggta gagcggtttg gcgaacccga
     3361 actcgctgct tttgccctcg gtatcgatcc gatcgctgcc catcaaaaag tcacccgcgg
     3421 gaatgaggac catgtcgggt ggactcggag tgcaggccgc gagtgcgaga aagagcaaaa
     3481 ccgagaggga gagggggggc ggggtgaaag gacatagaga tggttctttt ctcgcccatt
     3541 ctcccactct cccattcacc ccctcgatct cttcgctcac cgagcgcacc ggaacccgat
     3601 gtcccggaac cgttgctcgg gggcgtgata gaagcggtac gcggcgcggt agaagtgcgc
     3661 cacggtgtag tggccggctg atccgcccca actgctgttg cgcacgctcc gaaacttctg
     3721 gccgaacgcc gggtttgcat aggtggaccc ggggtacgcg tgataccagt ccccgaccca
     3781 ctcggacacg ttgcccgcca tgtcgtacac tccgtacgga ctgacgccct gcggaaagct
     3841 gccgaccggc gcaaggcccg tgcccccgat gttgcccttc gtggtatcga actcgtttcc
     3901 ccacggatat tcccgtccgt cggtcccgcg cgcggccttc tcccactcgg cttcggtggg
     3961 cagtcgtttt ccggcccatc ggcaaaaagc gtcggcctca aaccaggtga cgccggtgac
     4021 gggccaggtg gcgtgctccg gagcaaagcg gccatccggc cacgtggggg gcgcgggcgc
     4081 gcgggtcgcg tcaacgtatg cgcggtactg ctggttcgtc acttcgtagg cgtcgatctg
     4141 gtaggcgggc agcgtgaccg tgcgtttggg gtgttcgtcc agaaacaagg gcttcgcggt
     4201 cccaaactcc gaaccttgct gcgcggtatc gatctgatcg ctccccatga tgaagtcgcc
     4261 ctgggggatc acgaccatgg cgccgatctg gggtggactg gccgcgtcgc ggcccgtggc
     4321 gcccttgcat cccaccgtga tggccagcgc cgcgagcagc gcgaccacaa aagctcgcgt
     4381 cacagaaaca acccgaaccg gttgaagaac gccttcaacc acccgcggtc agggtcggaa
     4441 aaccggctga ccccgacgcg ggtcccgaac cacttgttgc cgtcctcatc caccgcgatg
     4501 gagtacacga aattatcgat caggccgtcg cgggtggtgt aggtgcgcca gtggttgccg
     4561 tcgaaccaac tcgcgccgcc gtcggtcccg gcccataaga ggccgttgtg atcaacggtc
     4621 aacgcaaaga caaaattgcc gccgaggcca tcggctttgg aatacgtggt ccacgttcgg
     4681 ccgtcgaagc gcgagacgcc cccgccgtag gtcccgaacc acttgtggtc gttgcggtcg
     4741 attgccgcgg cgaccacgta gttggggttg gtcgccgcgg cctgtttgga ggggtccaag
     4801 tggtgcgggt tgccgaacgc gtaggacggg tccttcacga cttcgccggt gtccaatccc
     4861 agcccatcgg cgtgcgtata gcttttccaa ttcgtgccgt cgaaacgcga cacgccgccc
     4921 tcggtgccga accacaggac accctgttga tcaatcgcaa tggcgtagac ccatttgtcg
     4981 acgagtccgg cgtccaccgt atatgtggta aaccccgcgc cgtcaaagcg actgacgcct
     5041 ttccaggttg cgacccacat ttgtcccttg cgatcgaatg cgacgtcgta gacccagaga
     5101 tccccgagcc cgcgacccgg ggtgtaggcg gtccaactct tttggtagtc gatcgtggaa
     5161 cccgatccat acgggttata attcgtccac gtcgtgccgt cgaaacgata gagcccgcct
     5221 ccataggttc cgatccattt atcgccttgc cgatcgatct cgatcgtata gatgccgttg
     5281 ctgaggattc ggcctcctgt attggccggc gtgtagagat cgaactgctt ctccgtgcgc
     5341 agatc
//
LOCUS       DBNGTT010000033         6457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_261948, whole genome shotgun sequence.
ACCESSION   DBNGTT010000033 DBNGTT010000000
VERSION     DBNGTT010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_261948"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1328)
                     /locus_tag="AC16BC_00755"
     CDS             complement(135..1328)
                     /locus_tag="AC16BC_00755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3240168.1"
                     /translation="MSIRLSRLASTFHAETNPLYRERDQRLAQGLPVVDLVSGNVTEQ
                     GIAFPPDLLRSILADAAGSAQTYRPVPFGQLPAREAVSAYYRAQGVDVPVAQIVLTPG
                     TSLAYWYAFSLFCDPGDEVLTPRPSYPLFDDIATLANVRVTSYRLDETRGWAIDLTAL
                     DAAITDRTRAIVLISPHNPTGAVATREEIHGLAAVARERGLPIIADEVFGEFLFGLDA
                     LPRPAATDAPLVVTLNGMSKMFALPGMKLGWMTLTGAAADVARSLGALERMSDAFLPV
                     NETAQAAAPRIMSEGRHFLARYRAVIESRGRDALAVIRESPRVSLVSPRGGFYASLRV
                     DTAWDDETLALHILRASGVLVHPGFFYDLDPTHIVLSLVSAPEGGLDAIRRVLDAIAT
                     APPGD"
     gene            complement(1332..2333)
                     /locus_tag="AC16BC_00760"
     CDS             complement(1332..2333)
                     /locus_tag="AC16BC_00760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3240169.1"
                     /translation="MSTSIRTRTLGRTGLAVSELGFGAWGIGGGAWGRGDDALSLAAL
                     EKALQLGITFFDTAHAYGDGRSERLIAEAAKKLGVRRKITVATKIPPKNREWPARHNA
                     PIRDVFPKDWIIRCTEESLRHLDSDFIDLQQLHVWSPQWIQDTEWIDTLRDLQLAGKV
                     RYLGVSINDHEPDTALELVRSGWIDTIQVIFNLFDQRPAVRLLPVAQEHHVGIIARCP
                     FDEGGLTGALRPDTTFARTDWRRDYFKGNRLAETVRRADALKAAVGNDYETLAHAALQ
                     YVLSHKILSEPSVATVIPGMRRPDHVEANVRYAMGPAFTLDMMKRVEAHAWGRNFYD"
     gene            2551..2790
                     /locus_tag="AC16BC_00765"
     CDS             2551..2790
                     /locus_tag="AC16BC_00765"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3240170.1"
                     /translation="MAKVQLYTTDFCHYCRVAKALLDDAKIPYEEINVSGTPEMRDVL
                     VKKTGEWTVPQIFVDGQYIGQDDELREWIKSGRLK"
     gene            2787..3482
                     /locus_tag="AC16BC_00770"
     CDS             2787..3482
                     /locus_tag="AC16BC_00770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3240171.1"
                     /translation="MSGPRDPSKVQAPPLEIETGRNPVAAVIWLHGLGADGHDFEPIV
                     PELRIPSALPIRFIFPHAPHRPVTINNGYVMRAWYDIRTAEFVQREDEAGIRESEQAV
                     RDLIAREIARGIPANRIVLAGFSQGGAIVLHVGLRYPERLAGIMALSTYLPLADLFPN
                     EAHPANRTVPIFMAHGTDDPIIPVERGQLSAGLLKNAGADVLWRTYEMGHSVCIEEIG
                     DIAAWLVRTLTAS"
     gene            complement(3507..4073)
                     /locus_tag="AC16BC_00775"
     CDS             complement(3507..4073)
                     /locus_tag="AC16BC_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3240172.1"
                     /translation="MPITIGKKPESSFADPLGLLSDCHRRIERFLGLLITVTADARGG
                     ALSAEQREALVAAGRYFREAAPKHIEDEERSLFPRMRDSPDRQVRAALATIKELEEDH
                     VVVDRAHIEVDTLVERWMDEGSLPASDARRLVTVLEELRTIYERHIAIEDHDVFPLAG
                     RILNATEIATLGREMAERRGLKPNDAWG"
     gene            4345..5109
                     /locus_tag="AC16BC_00780"
     CDS             4345..5109
                     /locus_tag="AC16BC_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3240173.1"
                     /translation="MTTQTGTEFNRRASLDEQIDRFRAQLGRLDPLLTPLTSASTFVD
                     FDAATEQLIAEAFGSTSDNLETYYYAQLGEAASMLNLQEDAPEGSHDDVGRESLRQRR
                     RVLESCIWELEAKRAAIAKKRTPVPQVATRVAEYMTRAIRSVPADSTLRDAGVRMEDN
                     KIACLFVSDQQRYVGTLTDAMLSRAVVVRGLDPAITPVKRCMDDAIVSIDNQEPMVEA
                     VKLMKDRGVRHLAVTDGDAIVGVLSVSDLLRYYSGV"
     gene            complement(5150..5542)
                     /locus_tag="AC16BC_00785"
     CDS             complement(5150..5542)
                     /locus_tag="AC16BC_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240174.1"
                     /translation="MLLLCAARQAAANPNSADFQHLGVATYPNAVLRQSDHRVAAESA
                     TDGTLFQWVSNDAPQAVVAFYEKSTGATAAFMKMSSTYTLYTPQGTMINVVGPEDGIP
                     EIDAGGRTTKTWKSLITIMRFDRIESNQ"
     gene            complement(5632..>6457)
                     /locus_tag="AC16BC_00790"
     CDS             complement(5632..>6457)
                     /locus_tag="AC16BC_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240175.1"
                     /translation="HPVWVNANIALDSADTVHVSYLDFPGLTHAVYGSGAWTAEVADA
                     QAWCDASLAIDSTDKIHLAYRASSSPNDEVRYASNTSGAWTSQVVDTSTSVGCDVAGR
                     RVSIAVDASGAAHMVYAGNSPDYGLKYATNQGGPWTVSVVNQDYIEQVSAAVDATGTV
                     HVAYANNVSQLRYAHNALGTWTTETIETAGSTSYPSLAVDAAGTAHISYFHSGYGELR
                     YAHNSSGTWQTVAIADVGPAPQNSGSDTAIALDSQGKAHIGYFDNRTGSLRHATNR"
BASE COUNT         1100 a         2039 c         2204 g         1114 t
ORIGIN      
        1 cacgccgcgg ccgcgaatag gacgaatgcc gcgagggtga cgatccacgt gggtttcatg
       61 acggctcctt tcggttcggg gtcacgcccc cttcctgcgg ggcgcgcgcg atcatatcag
      121 gatggacgag cgtgtcaatc ccccggtggc gcggtcgcga tcgcgtccag cacgcggcga
      181 atcgcgtcga gcccgccttc cggcgcgctc accaggctca ggacgatgtg cgtcgggtcg
      241 aggtcgtaga aaaatcccgg gtgcacgagc accccgctcg cgcggaggat gtgcagcgcc
      301 aaggtctcat cgtcccacgc ggtgtccacg cgcagcgacg cgtaaaatcc gccgcgggga
      361 ctgaccaacg acacgcgagg ggattcccga atgacggcga gcgcgtcgcg cccgcgggac
      421 tcgatcaccg cgcgatagcg ggcaagaaaa tgacgtccct cggacatgat gcgcggcgcc
      481 gcggcctgtg cggtctcatt gacgggcagg aaggcgtccg acatccgttc gagcgcgccc
      541 aggctccgcg cgacgtcggc ggcagctccg gtcagagtca tccaccccag cttcatgccc
      601 ggcaaggcga acattttcga catcccgttg agcgtgacca ccagcggcgc atccgtggcc
      661 gccgggcgcg ggagtgcgtc gagtccgaac agaaactcgc caaacacctc gtcggcgatg
      721 atggggagtc cgcgttcgcg cgccaccgcc gcaaggccgt ggatttcctc gcgcgtagcg
      781 accgcgccgg tggggttgtg cggtgagatc aggacgatcg cccgcgtgcg atccgtgatc
      841 gccgcgtcga gggcggtcag gtcgatggcc cagccgcgcg tttcgtccag ccggtacgag
      901 gtgacgcgga cgttcgcaag ggttgcgatg tcgtcgaaga gcggatacga gggccgcggc
      961 gtgagcacct cgtcgccggg atcgcagaac aacgagaacg cgtaccagta cgcgaggctg
     1021 gtgccggggg tgagcacgat ctgcgcgacc ggaacatcga cgccttgggc gcggtagtac
     1081 gcgctgaccg cctcccgcgc ggggagttgt ccgaatggca ccggccgata ggtctgcgcg
     1141 gagcccgcgg cgtcggcgag gatggagcgg aggaggtcgg gcgggaaggc gatgccctgt
     1201 tccgtgacat tgcccgacac caaatccacg accggcaggc cctgcgcgag gcgctgatcc
     1261 cgctcgcgat agagcgggtt ggtctcggcg tggaacgtcg atgcgaggcg ggacagccgg
     1321 atcgacacgg atcagtcgta aaagtttcgg ccccacgcgt gggcttcgac ccgcttcatc
     1381 atgtcgagcg tgaaggccgg gcccatggcg taacggacgt tggcttccac gtggtcgggc
     1441 cgtcgcatgc ccgggatgac cgtggccacg gacggttcgc tcaggatctt gtggctcagc
     1501 acgtattgga gcgcggcgtg ggcgagcgtt tcatagtcgt tcccgacagc ggccttgagc
     1561 gcgtcggcgc ggcggacggt ttccgcgagg cgattgccct tgaagtagtc gcgccgccaa
     1621 tcggtccggg caaaggtcgt atccgggcgc agcgcgccgg tcagtccgcc ctcgtcaaaa
     1681 gggcaacgcg cgatgatccc gacgtggtgt tcctgcgcca ccggcagcag ccggacggcc
     1741 gggcgttggt cgaagaggtt gaaaatcacc tggatcgtgt cgatccagcc ggatcggacg
     1801 agttcgaggg ccgtgtccgg ttcgtggtcg ttgatcgaga cacccagata ccggaccttg
     1861 ccggccagtt gaaggtcacg caacgtgtcg atccactcgg tgtcttggat ccactgcggc
     1921 gaccagacgt gcagctgttg caggtcgatg aagtcgctgt cgaggtggcg caggctctcc
     1981 tcggtgcatc ggatgatcca atccttgggg aacacgtcgc ggatcggcgc gttgtgccgg
     2041 gcgggccatt cgcgattctt gggtggaatc ttggtggcga cggtgatttt gcgccgtacg
     2101 ccgagtttct tcgcggcctc ggcgatgagc cgctcgctgc gcccgtcccc gtaggcgtgc
     2161 gcggtgtcga aaaacgtgat gccgagttgc agcgccttct ccagcgcggc gagcgacaac
     2221 gcgtcgtccc cgcgtcccca cgccccgccc ccgatacccc acgcgccgaa cccgagttcg
     2281 gacaccgcga gcccggtgcg gcccaacgtg cgcgtgcgga tgctcgtgct caccgccgca
     2341 gaataacaag gcggccggac agagggcaag tgacttggtt gtctggtagc ggaacagttg
     2401 tcgcgtgtgt tcactccttc cccccttgcc cccaccccat ccctcccccg caagggggga
     2461 gggagtcaca gggccaacgt cgcttgccgg agattgaggg ggcgtccgct ggcctggtac
     2521 acggcgtcac gctgtgatag catgccgcgt atggccaagg tgcagctgta cactacggat
     2581 ttttgtcatt attgccgcgt ggcgaaagcg ttgctcgacg acgcgaagat tccctacgag
     2641 gagatcaacg tctccggaac gcccgaaatg cgcgacgtat tggttaaaaa gaccggggag
     2701 tggaccgtgc cgcagatctt cgtggacggg cagtatatcg gtcaggacga tgagctgcgc
     2761 gagtggatca agtcgggtcg gttgaaatga gcggcccgcg cgatccgtcg aaggttcagg
     2821 cgcccccgct cgagatcgaa accgggcgta acccggtcgc cgcggtcatc tggttgcacg
     2881 gcctcggggc cgacggccac gacttcgagc ccatcgtgcc ggagctgcga attccgagcg
     2941 cgctcccgat ccgctttatc tttccccacg cgccgcaccg gccggtgacg atcaacaacg
     3001 ggtacgtgat gcgcgcgtgg tacgacatcc gaaccgcgga gttcgtgcag cgggaggatg
     3061 aagcgggcat tcgggaatcc gagcaggccg tgcgcgacct gatcgcgcgg gagatcgcgc
     3121 gcggcatccc ggccaaccgc atcgtgctcg cggggttttc ccaaggcggc gcgatcgtgc
     3181 tgcacgtggg gctgcgctac ccggaacgcc ttgccggcat catggcgctc tcgacgtacc
     3241 ttccgctggc cgatctgttt ccgaacgagg cgcaccccgc caaccggacc gtgccgatct
     3301 tcatggcgca cggcaccgat gatccgatca tccccgtcga gcgcggccaa ctgtccgccg
     3361 gtctgctcaa gaacgccggg gccgacgtcc tgtggcgaac ctatgagatg gggcactcgg
     3421 tctgtatcga ggagatcggc gacatcgcgg cgtggctggt gcggaccctg acggcgtcgt
     3481 aacgccggat cacgcgagtc gggcggtcag ccccaggcgt cgtttggttt gaggccgcga
     3541 cgttcggcca tttcccggcc cagcgtggcg atttccgtcg cattcaagat tcgtcccgcg
     3601 agcgggaaca cgtcgtggtc ttcgatcgcg atgtggcgct cgtagatcgt ccgcagttcc
     3661 tcgagcacgg tgaccaggcg ccgggcgtcg gacgcgggta acgagccctc gtccatccac
     3721 cgctccacca gcgtatcgac ctcgatgtgc gcgcgatcca ccaccacgtg atcttcctcc
     3781 aactccttga tcgtggccaa ggccgcgcga acctggcggt cgggtgaatc ccgcatccgg
     3841 gggaacagcg agcgttcctc gtcctcgatg tgtttgggcg cggcttcgcg aaaataccga
     3901 ccggcggcga ccaaggcctc gcgctgttcg gcgctcaacg ctccgccgcg cgcgtccgcg
     3961 gtcaccgtga tcaacaatcc caggaagcgc tcgatccggc gatggcaatc gctgagcagg
     4021 ccgagcggat cggcgaacga actctcgggt ttcttgccga tggtgatggg catggcgggt
     4081 ctccgggacg agcagagcgt tggcgattcg tattgtacca cgtggggagg cggggagaaa
     4141 gccgagcatc ggcgccggct tgcaaccgcg ttgaagtagg attggcgaac ggtccggccc
     4201 tccccggggg gagcccatgt gcgaacctgc ccgggtgagc ttgacagggc gttatcggaa
     4261 gccggacaat ccaacacggg acgagcgcgg acgttccgac cccttccctc cgcgagttga
     4321 aacccctcac cctgcgaggg cgccatgacg acacaaactg gcaccgaatt caaccggcgg
     4381 gcgtcgcttg acgagcagat cgaccgtttc cgcgctcaac tcggacgcct ggatccgctg
     4441 ttgacccctt tgaccagcgc gtccacgttc gttgattttg acgccgcgac cgagcagttg
     4501 atcgccgagg ctttcggcag tacgtcggac aacttggaga cgtactacta cgcgcagctc
     4561 ggggaggccg cgagcatgct gaatctccag gaggacgccc cggaagggag ccacgacgac
     4621 gtcggccgcg agagcctccg gcaacgtcgc cgggtcctgg agagctgtat ctgggaactc
     4681 gaagccaaac gggcggcgat cgccaagaaa cggaccccgg tcccacaggt cgccacgcgg
     4741 gtcgccgaat acatgacccg ggcgatccga agcgtccccg cggactcgac gttacgcgac
     4801 gccggcgtcc gcatggagga caacaagatc gcgtgcctgt tcgtgagcga ccaacagcgc
     4861 tacgtgggca cgctgaccga cgcgatgctc agccgagccg tcgtggttcg gggactcgat
     4921 cccgcgatca cgccggtgaa gcgctgtatg gacgacgcga tcgtgtcgat cgacaaccag
     4981 gagcccatgg tcgaggcggt caagctcatg aaggaccgcg gcgtgcggca tttggcggtg
     5041 accgatggcg acgcgatcgt gggcgtgctc tcggtgtctg atctgctcag atactattcc
     5101 ggcgtgtgac cgcgccggga gcgcggggca atcgagaaac ggacgcgacc tactggttgc
     5161 tctcgatgcg gtcgaaccgc atgatggtga tgagcgactt ccacgtcttg gtcgtccgcc
     5221 cgcccgcatc gatctcgggg atgccgtctt ccggacctac cacgttgatc atggtgccct
     5281 gcggagtgta cagcgtgtac gtcgaggaca ttttcataaa cgcggcggtc gctccggtcg
     5341 atttctcata aaacgccacg accgcctggg gcgcgtcgtt cgacacccac tgaaacaacg
     5401 tgccgtcggt ggcgctttcg gcggcgaccc ggtgatcgga ctggcgcaga accgcattgg
     5461 gataggtggc gacgccgagg tgctggaagt ccgctgagtt tgggttggcc gcggcctgac
     5521 gagcggcgca caaaaggagc acggcgatga ttgcgatagg ggatagtctc atggtcgggc
     5581 tccgattagg cgaagtcgga gtataccgga gtccagtgcg gcgtcaatca gctacctgtt
     5641 ggtcgcatgc cttaggctgc cggtccggtt gtcgaaatac ccgatgtgcg ccttgccctg
     5701 cgagtccagg gcaatggccg tgtccgaccc cgaattttgg ggcgcggggc cgacatcagc
     5761 gatcgcgacg gtctgccacg ttccggagga attatgggcg tatcgaagct caccgtaccc
     5821 ggagtggaag tagcttatgt gcgccgtgcc ggctgcgtcc acggcgagcg acggatagct
     5881 ggtagaaccg gcggtctcga tggtctcggt tgtccacgtc ccgagcgcgt tgtgcgcgta
     5941 gcggagctgg ctgacgttgt tcgcgtacgc gacgtgcacc gtgccggtgg cgtccaccgc
     6001 cgcggacacc tgctcgatgt aatcttgatt cacgaccgag acggtccacg gccctccctg
     6061 gttggtcgca tacttgagtc catagtccgg ggagttcccg gcatagacca tgtgtgcggc
     6121 tcccgacgca tccacggcaa tggacaccct tctgccggcg acatcgcacc ccactgaagt
     6181 ggacgtgtcc acgacctgcg acgtccacgc gccggacgtg tttgaagcgt atcggacctc
     6241 gtcgttcggc gacgagctcg cgcggtaagc gaggtggatc ttgtcggtcg aatcaatggc
     6301 cagcgacgcg tcgcaccatg cctgcgcgtc ggccacctcc gcggtccagg cgcctgagcc
     6361 gtagaccgcg tgcgtgaggc cggggaagtc caggtagctc acgtgcaccg tatcagccga
     6421 atccaaggcg atgttggcgt tgacccacac cggatgg
//
LOCUS       DBNGTT010000034         2409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1922917, whole genome shotgun sequence.
ACCESSION   DBNGTT010000034 DBNGTT010000000
VERSION     DBNGTT010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1922917"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="AC16BC_00795"
     CDS             <1..701
                     /locus_tag="AC16BC_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240176.1"
                     /translation="GIIQRRVIDALQRDPPPVAWIGPREGNPASLRAYRLYRWLLERG
                     YRFHEADGYQFLVRADRADHLGLGVESDRDRAELRRIFAPTDLGRLPQAWGRALGNPE
                     SRFRPLPASLAAGPIDPALRTEDPTRVWRLNTPIRGRDADFAAIHVSCGRALSDPIIA
                     QALWSSDAEAWTPEHSLTFRLAAGELLLPMGAHPDWLRGEGIARLAVRVDRAIVSRCG
                     TVRVGPPRLLRLTD"
     gene            complement(710..2149)
                     /locus_tag="AC16BC_00800"
     CDS             complement(710..2149)
                     /locus_tag="AC16BC_00800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3240177.1"
                     /translation="MLNSIAGWLVIWVVVTVSCGLGALEVFLYERQREASMAMIDEAL
                     HSHAELFASLIEIFPDGSVHFEPAEHQDRYPTLFDYQGAGHYYQISDDAGKVLARSRS
                     LDDWAFDIPYGRLYAPYYGTLVAPDGDRVRVHARRVIVSPLGQPAALRTFIISAGSSL
                     RDMEGFLALLRTTMLYSAPFIVLFSALGGYLISRASLRPLERFSSEIGQISEQTLDRR
                     VREDRVSSELRDLAAAFNAMLGRIQTVFQQQRRFISDASHELRTPVSVIKSYCEVPLR
                     RDRSVEEYKQRLATILHHTEKLSTLINNLLTLSRLEHRSLPLTKERVDLNRAVGATAA
                     LVAPLAKRKDITVRCQLAPHAVTAWGDETALTEVFTNLIENAIKYTGEGGCVTVRVES
                     DANEAVVAVEDTGVGIPADALPHLFDRFYRGDLSRTQESATGANGYSGFGLGLSIVKE
                     LVDAHQGRLTVTSEVGKGSVFTVRLASAA"
     gene            complement(2142..>2409)
                     /locus_tag="AC16BC_00805"
     CDS             complement(2142..>2409)
                     /locus_tag="AC16BC_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3240178.1"
                     /translation="KREARLGGKLVDLTPKEFSLLEYLVRHRDRAVTRTEISEHIYDI
                     SFDSDSNVLDVHVNALRKKLARRSSPPVIETVRGVGYMIRTGRA"
BASE COUNT          397 a          832 c          768 g          412 t
ORIGIN      
        1 acgggatcat ccaacgccgc gtcattgacg cgctgcaacg cgatccaccg cccgtggcat
       61 ggatcggacc gcgcgagggc aaccccgcct cactgcgcgc gtatcgtctg taccgctggc
      121 tgttggaacg cgggtatcgg ttccacgaag cagacgggta tcagttcctg gtacgcgcgg
      181 atcgcgccga ccacctcgga cttggcgtgg aatcggaccg cgatcgcgcg gagttgcgtc
      241 gaatcttcgc gccgaccgac ctgggccggc tcccgcaggc gtggggccgc gcgctcggca
      301 atcccgaatc gcgattccga ccgctccccg cgtccctggc cgcggggccg atcgaccccg
      361 cgctccgcac cgaagacccg acccgggtct ggcgcctgaa caccccgatt cgcggccgag
      421 acgcggactt tgccgcgatc cacgtgtcct gcggtcgggc cctgtccgat ccgatcattg
      481 cccaggcgct ctggtcttcg gacgccgagg cgtggacccc ggaacattcg ctgacctttc
      541 gcctcgcggc cggcgagttg ctgctcccga tgggcgctca tcccgattgg ctgcgcggcg
      601 aaggaatcgc ccgcctggcc gtgcgcgtgg accgtgcgat cgtcagccgg tgcggaacgg
      661 tccgcgtcgg cccgccgcgg ttgttgcggt tgacggactg accaacgcgt caagcagcgc
      721 tcgccaggcg gaccgtgaac acgctgccct tcccgacctc gctggtcacg gtcagacgac
      781 cctggtgggc gtcaaccaac tccttcacaa tgctcagccc cagaccgaat ccggagtatc
      841 cgttcgcgcc ggtcgccgac tcctgggttc tggacaggtc cccccggtag aaccggtcga
      901 acaggtgggg taacgcgtcg gccgggatgc cgactcccgt gtcttccacc gccacgacgg
      961 cctcgttcgc gtcggattca acccgcacgg tcacgcagcc gccttcgccc gtgtatttga
     1021 tcgcgttctc gatcaggttc gtgaacacct cggtcagcgc cgtttcatcg ccccatgccg
     1081 tcacggcgtg cggcgccagt tggcaccgga ccgtgatgtc ctttcgtttc gccaacggcg
     1141 ccaccagcgc cgcggtcgcg ccgaccgccc ggttgaggtc cacccgctcc ttggtcagcg
     1201 gaagactccg gtgctcgagg cgcgacaggg tcaagaggtt gttgatcaac gtggacaact
     1261 tctccgtgtg gtgcaggatc gtcgccaggc gctgtttgta ctcctccacg ctgcggtcgc
     1321 gccggagcgg cacctcgcag tagctcttga tcacgctgac cggggtgcgc agttcgtgcg
     1381 aggcgtccga aatgaaccgt cgctgttgct ggaacacggt ctggatccgg cccaacatcg
     1441 cgttgaacgc cgcggcgagg tcgcgcagct cgctgctgac gcgatcttcg cgcactcgtc
     1501 ggtccagcgt ttgctcgctg atctggccga tctccgacga aaaccgctcc aacggtcgca
     1561 gggacgcccg cgagatcagg tacccgccga gcgcggagaa caagacgatg aacggcgcgc
     1621 tgtagagcat ggtcgttcgc agaagcgcga gaaacccctc catgtcccgc aggctcgatc
     1681 cggctgaaat gatgaacgtg cgcagggccg cgggttggcc caacggactc acgatgaccc
     1741 gacgggcgtg caccctcacc cggtccccgt ccggagccac gagggttcca tagtagggcg
     1801 catacaaccg gccgtacggg atatcgaacg cccagtcgtc gagcgagcgc gagcgagcca
     1861 gcacctttcc cgcgtcgtcg gaaatctggt agtagtggcc cgccccctgg tagtcgaaga
     1921 gcgtgggata acggtcttgg tgctcggcgg gttcaaagtg gaccgacccg tccggaaaga
     1981 tctcgatgag cgaggcgaac aactccgcgt gggagtgcaa cgcctcgtcg atcatcgcca
     2041 tggacgcctc gcgttgccgc tcgtagagga acacctcgag cgcccccagc ccgcacgaga
     2101 cggtcacgac gacccaaatc accaaccagc cggcaatgga attaagcacg gcccgtccgg
     2161 atcatatacc ccacgccgcg cacggtctcg atgaccggcg gcgaggagcg ccgcgccagt
     2221 ttcttgcgca acgcgttgac gtgcacgtcg agcacgttgc tgtccgaatc gaacgagatg
     2281 tcatagatgt gttcgctgat ttccgtgcgg gtgacggcgc ggtcgcggtg ccggaccaga
     2341 tactccagca gcgagaactc cttcggtgtg aggtccacga gtttgccgcc caaccgcgct
     2401 tcccgcttg
//
LOCUS       DBNGTT010000035         4102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1284505, whole genome shotgun sequence.
ACCESSION   DBNGTT010000035 DBNGTT010000000
VERSION     DBNGTT010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1284505"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="AC16BC_00810"
     CDS             <1..367
                     /locus_tag="AC16BC_00810"
                     /inference="COORDINATES: protein motif:HMM:NF017770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HIR3240179.1"
                     /translation="FGPYALALLLTVPTLGLVWFWFWARRTRYYWEHTSFATARFQST
                     MTAWRLFSLHAGNLLLLIVTLGVAWPWATIRKIRVEFANLSLVGPLDASLVKQDAQAA
                     TATGDSLGQFLDADMGLAA"
     gene            404..1588
                     /locus_tag="AC16BC_00815"
     CDS             404..1588
                     /locus_tag="AC16BC_00815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3240180.1"
                     /translation="MPEECAGFYLDGRTANRLRVTARILPDRLEIVTEAGETHAWPYP
                     RIRRTQGTYAGEQVRFEYGEPIAEALVVSDRNFLAALYRAAPRLGSHAHDPRRRTRRV
                     ALVAGAALAAIGLTVAVIRDGVPAIVKVVTPQIPLSWDVQLGDAVLEHFADAERRCDD
                     PALQAKVEGVFAATMGATVTPYRFHLIVLDDDEINAFAAPGGPIVILRGLLRKTASPE
                     ELAGVLAHEAQHVIKRHSTQALLQQASTGLLIAALTGDVSGAAAFGLNAAANLGALHY
                     SRSHETEADEEGMRLILAAGIDPAGMIAFYQLLAREGPELPGALSYLSTHPATDERVA
                     RLREIAEHAAPPRTTLGSRDNWEILRERCRAQGEFEDTAEQAEAADNARESPSSQESD
                     AR"
     gene            complement(1808..3502)
                     /gene="pheT"
                     /locus_tag="AC16BC_00820"
     CDS             complement(1808..3502)
                     /gene="pheT"
                     /locus_tag="AC16BC_00820"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3240181.1"
                     /translation="MPTITVLKSDLESLSGRKFSLDQLSDALALVKGELKEHVPATNE
                     LRIELNDTNRPDLWCVEGIARHLRKPKAGKAARIKASPAKSSYRIRVTKDVRAVRPYI
                     GACVARGVGVTDTLLTQLIQTQEKLAELYGRKRQTVSIGLYPLQKIVFPVSYTTSAPD
                     EASFVALGADRAMSLREILAKHPKGVAYGNILAAHSRYPLLVDSRGEILSFPPIINSQ
                     ALGAVAVGDDDLLVEVTGTDQRMVALAVNIWAQNLRDRGARVEPVTIEYAWATPMGRK
                     VVTPKEIAKPVSVPVQLIAQVLGEAIKPAEAKKLLTGYGHQVSGSGTTLKVTPPAHRD
                     DVLHPVDVVEDVAIARGYNSFAPEMPSQFTVGALSPIERYADRVRDLLVGQGFQETMA
                     NILTSRHDVVDRMGGGRNADGLVEVDNPMVETFAVLRPNLLASLLRAEAASSKAVYPH
                     RVFEVGEAASIDPASDVGCRTEVRAAVLIAHAEAAFSEAHAALESLAYALSVSYRLEP
                     TPHPSFIDGRAGAILVDDRPIGVIGEISPAVLDAWGIGMPCAVLDLSLHSLVGSSV"
     gene            complement(3509..>4102)
                     /locus_tag="AC16BC_00825"
     CDS             complement(3509..>4102)
                     /locus_tag="AC16BC_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA ligase subunit PheS family protein"
                     /protein_id="HIR3240182.1"
                     /translation="AGNVARAHETGKGTGSTGWRYPFDPGRARRLVLRSQGTAVSART
                     LAAKPKIPGKYFSIARCFRYDHVDATHAPDFFQVEGIVLGEDITFRTLLGLLRLFAVE
                     VARAPEVKFLPAYFPYTEPSVEMHAKHPTLGWMELGGAGLFRPEVTLPLGVKVPVIAW
                     GLGLDRMAMTALGLSDIRDLFTPDLETLRAQRAAVTL"
BASE COUNT          724 a         1372 c         1298 g          708 t
ORIGIN      
        1 cttcggcccg tatgcgctgg cgctcctgct caccgttccg acccttgggc tggtgtggtt
       61 ttggttctgg gcccgccgca cacggtacta ctgggagcac acgtcgttcg ccacggcgcg
      121 ctttcaatcc accatgaccg cgtggcggct cttcagcctg cacgccggaa accttctgct
      181 cttgatcgtc accctgggcg tggcgtggcc atgggccacg attcgcaaga tccgggtgga
      241 gttcgcgaac ctgtcgctgg taggaccgct cgatgcctcg ttggtcaagc aggatgcgca
      301 ggccgccacc gcgaccggcg acagcctggg acaattcctc gacgcggaca tggggttggc
      361 cgcctgacgt gatcgggcgc gccccagacc cgcgtcaccg atgatgccgg aggagtgcgc
      421 cgggttttac ctggacgggc gcaccgcgaa tcgtctgcgg gtgaccgcgc ggattcttcc
      481 cgaccgcctg gaaatcgtca ctgaggccgg cgagacgcac gcatggccct acccccgcat
      541 tcgccgcacg cagggaacgt atgcgggcga acaagttcga ttcgaatacg gcgagccgat
      601 cgccgaagcc ctggtcgtct cagaccggaa ctttctcgca gccttgtacc gggcggcgcc
      661 gcgcctggga tcccacgctc acgacccgcg acggcggacc cgtcgcgtgg cgctggttgc
      721 gggggccgcg ctcgccgcga tcggtctgac cgtggcggtg attcgtgacg gcgtccccgc
      781 gatcgtgaag gtcgtcaccc cgcagattcc cctgtcctgg gacgtgcagt tgggcgacgc
      841 ggtgctggaa cattttgccg acgccgagcg tcggtgcgac gatcccgccc tgcaggccaa
      901 ggtggaggga gtcttcgcgg ccacgatggg cgccacggtc accccgtacc ggtttcatct
      961 catcgtgctc gatgacgacg agatcaatgc gttcgccgcg cccggcggtc cgatcgtgat
     1021 cctgcgcggc ctcctgcgca agaccgcatc acccgaggag ttggcgggcg tgctggcgca
     1081 cgaggcgcag cacgtcatca aacgccactc cacccaggcc ctgctgcaac aggcgtccac
     1141 gggcctgctg atcgcggcgc tcaccggaga cgtaagcgga gccgctgcgt tcggactgaa
     1201 cgcggcagcc aacctcgggg cgttacacta cagccggagc cacgaaaccg aggccgacga
     1261 agaggggatg cggctgattc tcgccgcggg catcgatcct gccggcatga tcgcgttcta
     1321 ccaactgctc gcgcgggaag gacctgagct tccgggtgct ctgtcgtacc tgtccacgca
     1381 tccggccacg gacgaacgcg tagcgcgcct ccgcgagata gccgaacacg ccgccccgcc
     1441 gcgaaccacg ttgggatcgc gtgacaactg ggaaatcctt cgggagcggt gtcgtgccca
     1501 aggcgagttc gaggacaccg cggagcaggc tgaagcggcc gacaacgcac gagaatcccc
     1561 gtcatcccaa gaatccgatg cgcggtgacg gccgcgacgt gcgttcccgg ggtcaggaga
     1621 ggcggtcggg ttctgtgcgg tcgtagaaga ggtgctcgac caaaacgggg gacagactac
     1681 tctttcttgc ccgatcaacc accatgggct tgatcccggc tccggacgcg gcacgtttgt
     1741 atacactgta ctgaccggct cttccttcga tcagcgcaac gatgccctta ctgaccctcg
     1801 cctgcggtta aactgacgag cctacaagcg agtggagcga gagatctaag acggcgcagg
     1861 gcataccgat gccccaggca tcgagcacgg caggtgagat ctcgccgata acaccgatag
     1921 ggcgatcatc gacaagaatg gcgccggcgc ggccgtcgat gaaactaggg tgcggggtgg
     1981 gttccaggcg gtatgagacg ctcagcgcgt aggccaggct ctccagcgcg gcgtgggctt
     2041 cggagaacgc ggcttcggcg tgcgcgatca ggaccgccgc gcgcacctcg gtgcggcagc
     2101 ccacgtcgga cgcgggatca atgctcgcgg cttcgccgac ctcgaacacg cggtgcggat
     2161 acacggcttt ggagctcgcg gcctccgccc gcagcaaaga ggccagcaag ttgggacgca
     2221 gcacggcgaa cgtttcgacc atgggattgt ccacctcgac aagcccgtct gcgttgcgtc
     2281 cgccacccat gcggtccacc acgtcgtgcc gcgaggtgag gatgttagcc atggtttcct
     2341 gaaagccctg gcccaccaac agatcgcgta cgcgatctgc gtaccgttca atcggggaca
     2401 atgcgccaac cgtaaactgg ctcggcattt cgggcgcgaa cgagttgtag ccgcgcgcga
     2461 tcgccacgtc ctccaccacg tccaccgggt gcaaaacgtc gtcgcggtgc gcgggcggcg
     2521 tgaccttcaa cgtcgtaccc gatcccgaga cttggtggcc gtagcccgtg agcaatttct
     2581 tagcctcggc cggtttgatc gcttcgccca agacctgcgc gatcaattgc accgggaccg
     2641 atacaggctt ggcgatttcc ttgggcgtga ccaccttgcg ccccatgggc gtggcccagg
     2701 cgtattcgat cgtcaccggc tccacgcgcg cgccgcggtc gcgcaggttc tgcgcccaga
     2761 tgttgaccgc caacgccacc atgcgctgat ccgtgcccgt gacctcgacc agcagatcat
     2821 cgtcacccac cgccactgcg cccaaggctt ggctgttgat gatgggcggg aacgagagaa
     2881 tttccccgcg cgagtccacc aacaacggat aacgcgaatg ggccgcgagg atgtttccgt
     2941 atgccacgcc cttggggtgc ttcgccagga tctcgcgcaa actcatagct cgatcggccc
     3001 cgagagccac aaagctcgcc tcatccggcg cggacgtcgt gtacgacacc gggaacacga
     3061 tcttctgcaa cgggtagagg ccgatggaca cggtctggcg cttgcgtccg tacagttcgg
     3121 ccagtttttc ctgggtctgt atcaactgcg tcagtagcgt gtcggtcacg cccacgccgc
     3181 gcgccacgca cgcgccgata tacggccgca ccgcgcgcac gtcttttgtc acccgaatcc
     3241 ggtacgaact cttcgcaggt gacgccttga ttctcgccgc ctttcccgcc ttgggcttgc
     3301 gcaagtgcct cgcaattccc tccacgcacc acagatccgg ccggttggtg tcgttcagct
     3361 cgatacgcaa ctcattggtc gcgggcacgt gttctttcaa ctcgcctttg accaacgcca
     3421 gcgcgtcgga caactgatcg agcgagaact ttcgcccgga cagcgattcc agatcagact
     3481 tgagaacggt gatagtcggc atgcgggctt acagagtgac ggcggcgcgt tgcgcgcgca
     3541 acgtttccag atcgggagtg aagagatcgc gaatatcaga cagtcccaat gcagtcatcg
     3601 ccatccgatc gagtccaagg ccccacgcga tcaccgggac cttcacgcca agagggagcg
     3661 tcacttccgg ccggaacagt ccggcgccgc ccaactccat ccagcccaac gtcggatgct
     3721 tcgcgtgcat ttcaaccgac ggctcggtgt acggaaaata cgcgggcaag aacttcactt
     3781 cgggggcgcg cgcaacctcc accgcgaata gtctcaacag ccctaggagc gtgcgaaacg
     3841 tgatgtcctc gcccagcaca atgccctcga cctggaaaaa gtcgggcgcg tgcgtggcat
     3901 cgacgtggtc gtagcgaaag cagcgcgcga tggagaagta cttgcctggg atcttcggct
     3961 tggccgccag ggttcgggcc gacaccgccg tgccttggct gcgcagcacc agtcgccgcg
     4021 cgcggccggg gtcgaatgga taccgccagc ccgtcgaacc cgtgcccttg cccgtctcgt
     4081 gcgcgcgcgc cacgttgccg gc
//
LOCUS       DBNGTT010000036         3403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2434111, whole genome shotgun sequence.
ACCESSION   DBNGTT010000036 DBNGTT010000000
VERSION     DBNGTT010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2434111"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16BC_00830"
     CDS             <1..188
                     /locus_tag="AC16BC_00830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3240183.1"
                     /translation="IQTTKGNVLYLTVSLGAASFPDDATTGDELLQRADQAMYEAKRR
                     GKNQAVVYEAPGAPPQT"
     gene            395..559
                     /locus_tag="AC16BC_00835"
     CDS             395..559
                     /locus_tag="AC16BC_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240184.1"
                     /translation="MEYFAVEALNRYKEVVIYDSITQKLWYPGGFICIDAERELLRIL
                     LGRYGAPGRY"
     gene            complement(609..1520)
                     /gene="ftsY"
                     /locus_tag="AC16BC_00840"
     CDS             complement(609..1520)
                     /gene="ftsY"
                     /locus_tag="AC16BC_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3240185.1"
                     /translation="MSLWNRIVSGLAKSRDALATGFQSLLGGSSSASAVSFEDLEAAM
                     IQADIGIRTTERLLGEVKRAGDVEPDAIKRRLQSLIAASLQPTVASNGQMPEARPTVV
                     LMVGVNGVGKTTTVAKLAARARAEGRSVMLAACDTFRAAAIDQLRIWGERLGVEVVHQ
                     QPGSDPAAVAFDAVSAAKARGIDVVYVDSAGRLHTKHSLMDELTKVKRVIAKALPGAP
                     HEVLLVLDATVGQNGLAQARAFHAALGLTGIVVTKVDGSSKGGIVLAVVEELGVPVKW
                     LGLGESADALVPFDAEAFAYGLVGATS"
     gene            complement(1524..1970)
                     /gene="ybeY"
                     /locus_tag="AC16BC_00845"
     CDS             complement(1524..1970)
                     /gene="ybeY"
                     /locus_tag="AC16BC_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3240186.1"
                     /translation="MIQVSVSNRQRSVKLDTARMKRHARRILLEMGFVKAEFSLAVVD
                     DAEMRLLNATYRRINEPTDVLAFAMTEGRFSGINPQLLGDVVISAETAAYRAQRDGRP
                     LREELNALLIHGILHLIGYDHERSPADARIMRAKERSLRRLLESPA"
     gene            complement(2008..2805)
                     /locus_tag="AC16BC_00850"
     CDS             complement(2008..2805)
                     /locus_tag="AC16BC_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3240187.1"
                     /translation="MSRLAARLQEQPNLPSDEIADTIRSARRGRDDAAEIEPGETVLR
                     TKKRLLTPKSPHQREYLEALKSADIVVGIGPAGTGKTYLAMGMAVAALVRREVSRIVL
                     TRPAVEAGEKLGFLPGDMFAKVNPYLRPMYDALYDMMDVEHANRLVERGEIEIAPLAY
                     MRGRTLNDSFIILDEAQNVTPEQMKMFLTRLGFRSKAVITGDITQVDLPAQSPSGLIE
                     IQNVVKDIPGIRFVYFDEGDVVRHRLVQEIIKAYERYEGRRDNRRKR"
     gene            3112..>3403
                     /locus_tag="AC16BC_00855"
     CDS             3112..>3403
                     /locus_tag="AC16BC_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240188.1"
                     /translation="MTVIVRWVVPPVFCTLIVQFESAEPFGLKDTVKFGAAPRQPACV
                     NTTVDGTVAQFVTTVAQAVLPELALPCTNRYWLAPAVRLSWTWCSRLRDTVRP"
BASE COUNT          613 a         1092 c         1061 g          637 t
ORIGIN      
        1 cgatccagac cacgaaaggc aacgtgttgt acctgaccgt gagcctggga gcggcgagct
       61 ttcccgacga cgccaccacc ggcgacgaac tcctccaacg cgcagaccag gccatgtacg
      121 aagccaaacg gcgagggaag aatcaagccg tcgtctacga ggcccctggc gcgccgccac
      181 agacgtaatg cctcccggct cgagcgcgga tccaccgaac gcgccgtcgg gattccatcc
      241 aggactttag accgcgctat tcgcctccgc gtcagccgtt acgagccctt cgcgtgcaga
      301 gggggtccgg cttactaacg ggtccagaag aaaagccacc gccgtggtga aacacaccgg
      361 caactcccgc cttggcccca gttgcccaag agagatggag tattttgcgg tggaagcgtt
      421 gaatcgttat aaagaggtcg tgatctacga cagcatcacc cagaagctgt ggtatcccgg
      481 cggattcatc tgcatagacg cggagcggga attgctccgg atacttctcg gccggtatgg
      541 agctcccggt cgatactagc ggtctagcct gaggttcgtg gatttgctga gcgcgtccgt
      601 gaccacgctt agctcgtggc gcccaccaga ccgtaagcga acgcttcggc atcgaacggg
      661 accagcgcgt ctgcgctctc ccccagtccc agccacttga ccggcacccc cagctcttcg
      721 accacggcca gcacgatccc gcctttggaa cttccatcga ccttcgtcac cacgatgccg
      781 gtcagtccca acgccgcgtg aaacgcccgg gcctgggcca gaccgttttg ccccacggtc
      841 gcgtccaaga cgagcagcac ctcgtggggc gcgccgggca gcgctttggc aatcacccgt
      901 ttgaccttcg tcagttcgtc catcagcgag tgtttggtgt gcaagcgccc cgcgctatcc
      961 acgtagacca cgtcgatccc gcgcgcctta gccgcgctca ccgcgtcgaa cgcaaccgcg
     1021 gcggggtccg atcctggctg ctggtggacc acttcaaccc ccagtcgctc gccccagatg
     1081 cgcaattgat cgatcgccgc ggcacgaaac gtgtcgcacg ccgccagcat cacggagcgg
     1141 ccttcggccc gggcccgcgc cgcgagcttg gccacggtgg tcgtcttccc cacgccattc
     1201 acgcccacca tgaggacgac cgtgggccgc gcttcgggca tttgcccgtt tgaggcaacc
     1261 gtgggttgca gcgacgcggc gatcaacgat tgcaaccgac gtttaatcgc gtccggctcc
     1321 acgtcgccag cccgtttgac ctcgccgagc aggcgctcgg tggtgcggat gccgatatcg
     1381 gcctggatca tcgcggcctc aagatcctcg aaggaaaccg cggatgcgct cgacgatccg
     1441 ccgagcaggc tctggaaccc ggttgccagc gcgtcccggc tcttggccag gccggacaca
     1501 atgcgattcc acaacgacat ggatcaggcg ggcgactcga gcaggcggcg caggctccgc
     1561 tccttcgcgc gcataatgcg ggcatcggcg ggcgagcgtt cgtgatcgta tccgatcagg
     1621 tgcaagatgc cgtggatgag cagcgcgttg agttcctcgc gaagcggtcg gccgtctcgt
     1681 tgcgcgcggt acgcggcggt ttcggccgag atgactacgt cgccgagcag ttgcgggttg
     1741 atgccgctga agcgcccctc ggtcatggca aaggccaaga cgtcggtcgg ctcgttgatg
     1801 cgccggtacg tggcgttgag gagacgcatc tccgcgtcgt cgacgaccgc gaggctgaac
     1861 tcggctttga cgaagcccat ttcaagcaag atgcgccgcg cgtggcgttt catcctggcc
     1921 gtgtcgagct tgacggagcg ttggcgattc gaaaccgaaa cctggatcat ggcgcgagca
     1981 tcgcgacttg ggcggtgaaa ccgatcgtca ccgcttgcgg cggttgtctc gccgaccttc
     2041 gtagcgttcg taggctttga tgatttcttg gaccaggcga tggcgcacca cgtcgccctc
     2101 gtcaaagtac acgaacctga tcccgggaat gtccttgacc acgttctgga tttcgatcag
     2161 gcccgatggg ctctgcgccg ggagatccac ttgcgtgatg tcgccggtga tcacggcctt
     2221 ggaccggaag ccgagccggg tcagaaacat cttcatctgc tcgggcgtga cgttttgggc
     2281 ttcgtccagg atgatgaacg aatcattcag cgtgcggccg cgcatatagg cgagcggggc
     2341 gatctcgatt tccccccgtt caaccaaacg attcgcgtgc tccacatcca tcatgtcgta
     2401 gagcgcgtcg tacatcggac gcagatacgg attcaccttg gcgaacatgt ccccgggcaa
     2461 gaatcccagc ttttcgccgg cctcgaccgc aggacgcgtc agcacaatcc ggctgacctc
     2521 ccgtcgaacg agcgcggcca ccgccatccc catcgccaag tacgtcttgc cggtcccggc
     2581 cgggccgatg ccgaccacga tatcggcgct cttgagcgct tcgaggtact cgcgttggtg
     2641 cggggatttc ggagtcagca gacgtttttt cgtccggagc acggtctcgc cgggctcgat
     2701 ctccgcggcg tcgtcacggc cccgtcgcgc ggaacggatc gtgtcggcga tttcgtcgga
     2761 gggcagattg ggctgttcct ggaggcgagc ggcaagccga ctcaagagcc gctcggcgcg
     2821 ccgaaccgcg tgaggggcgc cgtcgatacg aagttcttca ccccgcgcgt ggattgtgac
     2881 cccgaatgca tcctcgacat cccgtaaatg ttggtcgaat gtcccgaaga gggtggaggt
     2941 attaagacct tcctgtagtt tgaggcgaac actcacgcgc ggggatgacc tccgtgtctc
     3001 gcgtgaatta tatcggcagg tcgcgagaaa gcacaagccg cgccgagaat ccacccgggc
     3061 gcgacaggat aggactgcgg ttattgaatt cggacccgac cggtattctg agtgaccgtg
     3121 atcgtacgct gggtggtacc gccggtgttc tgcacgttga tcgtacaatt tgaatcagcc
     3181 gagccgtttg ggttaaagga caccgtgaag ttcggcgcgg cgccgaggca gccggcttgc
     3241 gtcaacacca cagttgacgg cacggtggcc cagttcgtga caacggtggc ccaggccgtg
     3301 ctccccgagc tggcgttgcc ctgcaccaac cgatattggt tggcgccagc ggttaggttg
     3361 agttggacct ggtgttcacg attgagagac accgtccggc ctc
//
LOCUS       DBNGTT010000037         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2306596, whole genome shotgun sequence.
ACCESSION   DBNGTT010000037 DBNGTT010000000
VERSION     DBNGTT010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2306596"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..710
                     /locus_tag="AC16BC_00860"
     CDS             108..710
                     /locus_tag="AC16BC_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014693.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HIR3240189.1"
                     /translation="MEKSFDDVVDFHRHLCLDIAMGYRVGKALVREMGKELRNMKDVF
                     AIVENETCAVDAIQEFTGCTLGKRNLILAGTGKPGYILQNAKSGVSVRIYAKYWETFD
                     PDGSFGKRRKHAGSPDATLEEKQLFDRELNSKIKEILRAPEDVLFTIQRVTLPAPPKV
                     GKYKAEPCGNCGEHTNVERLVEVGGKKLCAACRNPVGAAR"
     gene            complement(786..>2017)
                     /locus_tag="AC16BC_00865"
     CDS             complement(786..>2017)
                     /locus_tag="AC16BC_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240190.1"
                     /translation="SDADDNTQDSIAYLNSRFLLTSAVSEDGVSGVVTLIATDTGGNG
                     NRLLGGDPTTGGAYGPSGSTVRMLEAYLTADFKSWTAKGGRAVFKLGNGIILDDSVDG
                     LWANFPIGGMDVTLGSLKLVEQTDMNVVTTIGNSGPGTGADADLYVVNLGLGKMMGDR
                     SSGSVFLAYLNDRDANLFGGGDPLVLGSDSDKATLWIVGLSGGVDAGQVKLKGELDYL
                     TGELNIGGAKEDLKGYNLVLGAKMNAGVLPLGIDLIYTSGDDDPATTSEHNVNGLNGN
                     YPVGIILTNVGAVSGDTKDGTCLSPNGGPLGGSSGCIDGAGLTALKASTGMVHGPHTI
                     DVAAIYAKSTKEPVAGGDTDIGIELDATVSTALTKRFSVLAGLGYLLAGDYFKSVSPA
                     GSSDDLMVGVLQASYTF"
BASE COUNT          410 a          643 c          568 g          396 t
ORIGIN      
        1 aacgagtgcc ttcgcggcgc gccatcccct atactaaatt ttattcgtga tgcacccgcc
       61 gtcgagggtc gcggaccgcg ccctccggct cactctgaga ggttctgatg gaaaaatcgt
      121 tcgacgacgt ggtggatttt caccggcacc tgtgcctgga tatcgcgatg gggtatcgcg
      181 tggggaaagc gctggtgcgg gaaatgggca aggaacttcg caacatgaag gacgtattcg
      241 cgatcgtgga aaacgagacc tgcgcggtgg atgcgatcca ggagttcacc ggttgcacac
      301 tcggcaagcg caatttgatc ctcgcgggca cgggcaagcc ggggtacatc ctgcagaacg
      361 ccaaatccgg cgtgtcggta cgcatctacg ccaaatactg ggaaacgttc gacccggacg
      421 ggtcgttcgg taagcgccgc aaacacgcgg gctcgcccga cgccactctg gaagagaagc
      481 agctcttcga ccgcgaactc aattccaaaa tcaaggaaat cctgcgcgcg ccagaagacg
      541 tcctctttac catccagcgc gtgacgctcc cggccccgcc caaagtcggc aagtacaagg
      601 ctgagccgtg cggcaactgc ggcgagcaca ccaacgtgga acgcctcgtg gaagtcggcg
      661 gcaagaaact ctgcgccgct tgccgcaatc ccgtgggcgc ggcacgctaa cttcagctcg
      721 ttctcaaaca aagggggggc cggcgaaagc cgccccccct ttgtcgttac ccgcgttgcg
      781 gagagctaga acgtgtagct tgcttgcagc acgccgacca tcaggtcgtc gctcgaacca
      841 gccggactaa cagacttaaa gtagtcgcca gccaataaat acccgaggcc ggccaacacg
      901 ctgaaccgct tggtcaacgc cgtgctgacg gtcgcatcca gttcgatccc gatgtccgtg
      961 tcgcctccag cgaccggctc cttcgtcgat ttggcgtaga tcgcggcgac gtcaatcgta
     1021 tgcgggccgt ggaccatccc cgtggacgcc ttgagggccg tcaaccccgc gccgtcaata
     1081 caccccgacg aaccgccgag cggcccgcca ttggggctga ggcaggtgcc gtctttcgtg
     1141 tcgcccgata cggcgccgac attcgtcaga ataatgccaa ccgggtagtt gccgttgagc
     1201 ccgttgacgt tgtgctcgct agttgtcgcg gggtcgtcgt cgccgctggt gtagatgagg
     1261 tcaatgccga gagggagcac accggcattc atctttgcac caaggaccag attgtagccc
     1321 ttaagatcct cctttgctcc accaatgttc agttcacccg tcaggtagtc caattcgcct
     1381 ttcaacttta cttggccggc atcgacccct ccagacaaac caactatcca gagcgtcgct
     1441 ttatcactat cggaacctag tactaggggg tcaccgccgc caaataagtt ggcatctcga
     1501 tcatttaggt acgccaggaa aacactcccg ctgctcctat cccccatcat ttttcccaac
     1561 ccaagattga cgacgtatag gtcggcgtcc gcacccgttc ctggtccgct attcccgata
     1621 gtagtcacga cattcatatc ggtttgctca actagcttga gtgacccaag cgtgacgtcc
     1681 atgccgccga tcgggaagtt cgcccacaga ccgtccacgg agtcgtcgag aatgatgccg
     1741 ttcccgagtt tgaacacggc ccgtccgccc ttcgcggtcc agcttttaaa atccgcggtc
     1801 agatacgctt cgagcatccg cacggttgac ccagatggac cataggcccc tccggtcgtc
     1861 gggtcgccgc caagcagccg gttcccgttg ccgcccgtgt cggtggcaat cagcgtgacc
     1921 accccgctca cgccgtcctc gctgaccgcc gacgtcagta ggaagcggga gttgaggtag
     1981 gcgatgctgt cttgcgtgtt gtcatccgcg tcggaaa
//
LOCUS       DBNGTT010000038         3327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3456552, whole genome shotgun sequence.
ACCESSION   DBNGTT010000038 DBNGTT010000000
VERSION     DBNGTT010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3456552"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC16BC_00870"
     CDS             <1..411
                     /locus_tag="AC16BC_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIR3240191.1"
                     /translation="DFEAALSSVSHDAQWGGAPGDVTHQTLGVTARIHFRMADYIQPH
                     LSAGLGFHRLVVEDGSQDVFGAVGDGVFTGMGLDLGAGLSGYLTQNVSAGATLVYHFI
                     RYTEAEGVRESGTVSPAVNGDGVGVIFRVVYYFL"
     gene            746..1147
                     /locus_tag="AC16BC_00875"
     CDS             746..1147
                     /locus_tag="AC16BC_00875"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009303311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR3240192.1"
                     /translation="MIALDLHPPFDGDYVAAPSSDPLHREAHAQLNAYVRGQLTSFSL
                     PIDPQGTPFQRAVWAALLNIPYGQTRTYGQIASAIGRPEAARAVGMACGSNPIGLVIP
                     CHRVVGADGSLTGYAGGLDLKAQLLQHEGVR"
     gene            1263..>3327
                     /gene="polA"
                     /locus_tag="AC16BC_00880"
     CDS             1263..>3327
                     /gene="polA"
                     /locus_tag="AC16BC_00880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR3240193.1"
                     /translation="MPKRLVLIDGHGYIYRAFFALPPLSTSKGLQTNAVYGFTNMLLK
                     IAREQRPDYLAVAFDSKGPTKRHEEFEAYKAHRPPMPDPMAQQLPYIERMVEAFNIPK
                     LVMEGYEADDLIGTIARQGETQGLDVTIVTADKDMLQLVSPRVTVFDTLKNRIFGEAQ
                     VMERFGVAPLLVPDVLGLMGDSTDNIPGVKGIGEKTAKKLVSEYGSVEDILARLSEIP
                     QAKLRASLEEHQEEARRSRRLATIEVNCPVALDLPRFARQEPDTERLVALCRELEFTG
                     LLKTLAPSQSAPSVVVPVVDGAAFEQAMGRAREAGAVTLGIAWTTGPAVDAEPAAVAY
                     AMPNGEGGVLDASSRVAPAIHGLTALLGDERVTIRAHDVKPLLLWATRRRIAMRCAIW
                     DSMLAAYLLNPNRSDQTLAPVALEYLSRTVPAADGPQAAADWCAAAEALVDVLTSRLA
                     ADGQTAVLERIELPLVPVLARMEARGIRVDGDLLASLSKELDGQMSGMMQRLYTLAGG
                     EFNVNSPKQLAEILFTRLGLPPIKKTKTGYSTDESVLTQLAAQHELPAEILAYRQLAK
                     LKSTYVDALPALINPRTGRIHNTFHQAVAATGRLSSSDPNLQNIPIKGPMGPRIRAAF
                     VPEPGYVLLSADYSQIELRILAHLSGDEGLIEAFRTDGDVHTDTARTLFNLPADAITP
                     AMRRAA"
BASE COUNT          570 a         1118 c         1048 g          591 t
ORIGIN      
        1 gacttcgaag ccgcgctctc aagtgtctcg cacgacgcgc aatggggcgg cgccccaggc
       61 gatgtcacgc atcagacgct cggagtcacc gcccgcattc atttccgcat ggccgactac
      121 attcagcccc atctctcggc cggactgggc tttcatcgcc tggtcgtcga ggacggttcc
      181 caggacgtct tcggcgcggt cggcgacggc gtgttcaccg gcatggggct ggacctcgga
      241 gccggcttga gcgggtacct gacccagaat gtgtccgccg gagcaaccct ggtgtatcac
      301 ttcattcggt acacggaagc cgaaggcgtg cgcgaaagcg ggacggtttc acccgcggta
      361 aacggcgacg gggtgggcgt catctttcgc gtggtctact attttctcta gcgggctcgc
      421 tccctgcgcc gcagactagg ccggacatgt tgttcagtcc gtcctcgttc attgatcagc
      481 atttgcttta catctccact gtccgtgtgg tacgagtatc gccaaggatg ggctgtcgac
      541 tcccctttag ggccgcaagc aacttacttc accacctcgg aggtctgcga tggtcatgac
      601 tctcccctcg ttgagcactg gacgacctct gggccgtccc tcccgcaccc gcagaacaac
      661 cagaagtacc ggtgactccc tggcgacggc cagctacgtg gagttgccgt gggggccggt
      721 ccgtgtggtc accgggccgg gtggcgtgat cgcgctcgac ctgcacccac cgttcgacgg
      781 cgactacgtg gccgcgccgt catccgatcc cctgcaccgc gaggcccacg cgcagttgaa
      841 cgcctacgtt cgcggacagc tcaccagctt ctcgctaccc atcgaccccc aaggcacccc
      901 gtttcaacgc gcggtctggg cggcgttgct caacatcccc tacggccaga cgcgtaccta
      961 cggccagatc gcatccgcca tcggccgccc ggaggccgcg cgcgccgtgg gcatggcgtg
     1021 cgggagcaat ccgatcggcc tggtgattcc gtgccaccgg gtcgtgggcg cggacggctc
     1081 gctcaccggg tacgcgggcg ggctcgatct aaaagctcag ttgctccaac acgaaggcgt
     1141 ccgctagcct cgggcttgtg cccacttgcc cccctttgaa aaagggggga tggggggatt
     1201 tgcagttcgt cccgcttttc ttgtattccc tcatacccgt agtgtagtgt tccgcacggc
     1261 ccatgcctaa acgactcgtc ctcatcgacg gccacggcta tatttaccgc gcgtttttcg
     1321 cgttgccgcc gctgtcgaca tcgaaaggcc tccaaaccaa cgcggtctac ggctttacca
     1381 atatgctgct caaaatcgcg cgcgagcaac ggcccgatta tttggcggtg gcgttcgact
     1441 ccaaaggccc caccaagcgc cacgaggagt tcgaggcgta caaggcgcac cggccgccca
     1501 tgcccgatcc catggcgcag cagctcccgt atatcgagcg catggtcgag gcattcaaca
     1561 tcccgaagct cgtgatggag ggctacgaag cggatgattt gatcggcacg attgctcgcc
     1621 aaggcgaaac gcaggggctc gacgtcacga ttgtgaccgc ggacaaggac atgctgcagc
     1681 tcgtgtcgcc gcgcgtgacc gtgttcgaca cgctgaaaaa caggattttc ggcgaggcgc
     1741 aagtcatgga gcggttcggc gtggcgcccc tcctggtgcc cgacgtgctc gggctgatgg
     1801 gtgattcgac cgacaatatt ccgggtgtga aaggcatcgg cgagaagacg gcgaagaaac
     1861 tggtgagcga atacggatcg gtggaggaca ttctcgcccg tctgtccgag attccccaag
     1921 ccaaactccg cgcctcgctc gaagaacacc aggaagaagc gcggcgctcg cgccgcctcg
     1981 ccacgatcga ggtgaattgt ccggtcgcgc tggatttgcc gcgttttgcg cggcaggagc
     2041 ccgacaccga gcgattggtg gcgttgtgcc gggagctgga gttcacgggg ttgctcaaga
     2101 ccctggcgcc aagccagtcc gcgccgtcgg tcgttgtccc ggtcgttgac ggcgcagcgt
     2161 tcgaacaggc catgggccgc gcgcgcgagg ccggagccgt caccctgggc atcgcgtgga
     2221 ccacggggcc ggccgtggac gcggagcccg cggccgtggc ctacgcgatg ccgaacggcg
     2281 agggcggtgt gctcgatgcc tcgtcccggg tcgctccggc gatccacggg ctgaccgcgt
     2341 tgctcggtga cgagcgcgtc accatccgag cccacgacgt caaacccttg ttgttgtggg
     2401 cgacccgccg gcgcattgcc atgcgttgcg cgatctggga ctccatgctc gccgcgtacc
     2461 tgttgaaccc caaccgttcg gatcagaccc tggcccccgt ggcattggag tacctgtcgc
     2521 ggaccgtgcc ggccgcggat ggtccgcagg cagccgcgga ctggtgcgcg gccgccgaag
     2581 ccctagtcga tgtgctgacc agccgcctgg ccgcagacgg tcagaccgcc gtgctcgaac
     2641 ggatcgaatt gccgttggtc ccggtgttgg cgcgcatgga agcccgcggc attcgcgtgg
     2701 acggggatct gctcgcgtcc ctgagcaagg aactcgacgg ccagatgtcg ggcatgatgc
     2761 agcggctgta tacgctggcg ggcggcgagt tcaacgtgaa ctcgcccaaa caactcgcgg
     2821 agatcctgtt cacccgcctg gggctgccgc cgatcaagaa gaccaagacc ggctactcga
     2881 cggacgaaag cgtgctcacg caactcgcgg cgcagcacga gttgcccgcg gagattttgg
     2941 cctaccggca gctcgccaaa ctcaagtcaa cgtatgtcga cgcattaccc gcgctgatca
     3001 acccgcgcac cggtcgcatc cacaacacgt tccaccaggc cgtggccgcc acgggccggc
     3061 tctccagcag cgaccccaac ttacagaaca tcccgatcaa agggccgatg ggaccgcgca
     3121 tccgcgccgc gttcgtgccc gagccaggct atgtcctgct ctcggccgat tacagccaga
     3181 tcgagctccg catcctggcc catctttcgg gcgacgaagg gttgatcgag gccttccgga
     3241 ccgacggcga cgtgcacacc gacaccgcgc gcacgctgtt caacctgccc gctgacgcga
     3301 tcacccccgc catgcggcga gcggcca
//
LOCUS       DBNGTT010000039         3098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1923942, whole genome shotgun sequence.
ACCESSION   DBNGTT010000039 DBNGTT010000000
VERSION     DBNGTT010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1923942"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16BC_00885"
     CDS             <1..375
                     /locus_tag="AC16BC_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3240194.1"
                     /translation="MAGRRFAPVFTQNFLSNLDAIEAFLTPEGITAFRRLLDRLFDDI
                     VPMLTRFPDSGRPLLHHPVRSREARTLVRKLRALISAPDEVREFVLDDYLLLYLRRGR
                     TLVFLAIKHHRQLSFDLRRFWT"
     gene            complement(647..1780)
                     /gene="dinB"
                     /locus_tag="AC16BC_00890"
     CDS             complement(647..1780)
                     /gene="dinB"
                     /locus_tag="AC16BC_00890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR3240195.1"
                     /translation="MPPRIIAHLDMDAFFAAVEERERPHLKGLPIAVGADPMDGKGRG
                     VVATANYPARVYGLHSAMPITQAWRRSEWARRQGKPAVVFLRPHFEQYRETSGRIRAI
                     VERHASSIEQAGIDEMYIDLSTAGTYERAQAIAIAIKRDIATEEHLTASIGIGPNKLI
                     AKIASDARKPDGLTVVEDTDAEAFLAPLSVRKIPGIGPKTEITLRQQGITKVQDLKRF
                     SEAELVERFGKWGRALHERVRGRDEDPVQESDEVKSIGEEETFLRDTLDARMLMEHLK
                     AMARDVVARLTEAGYASFRTVVIKVRFADFETKSRSHTLPRPTTSGKDLEVEAMKLFL
                     PFLDRRENPRRKLVRLIGLRVEKLGREASGTSTGKRAGSQLLV"
     gene            complement(1967..2344)
                     /locus_tag="AC16BC_00895"
     CDS             complement(1967..2344)
                     /locus_tag="AC16BC_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240196.1"
                     /translation="MSAKTPPFRIQEEPCDQDSSEADRKVQAAEPHRGRIRAGSSRSI
                     PLTVENRLLKTARLAPGDPLAEVSDLAMRHDPEVDAYCLQLLPAPDEPPLAEYWFGNL
                     EQAERQAVLVFGARFTGWSEGTG"
     gene            2472..3080
                     /locus_tag="AC16BC_00900"
     CDS             2472..3080
                     /locus_tag="AC16BC_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR3240197.1"
                     /translation="MKKIRNTMLTGLIALLPLYLSFVLLVWLFKTLDALSQPWLARVF
                     HVTVPGLGIAATALVVFVTGLIVSSVSGALALSWVDRLFERVPLVKGLYSGIRQIIES
                     FNPANPSGFKEFVLVEQPQSRDLHAGFLTGEFALIKPDGTRREFATVYVPSNHLYLGT
                     IQIVERTRIIRTSMTLQDGITFTLSAGASAKGAFRESDAAAR"
BASE COUNT          524 a         1026 c          929 g          619 t
ORIGIN      
        1 atggccggta ggcggttcgc cccggtcttt actcaaaact ttctctccaa tctggacgcg
       61 atcgaggcct tcctcacgcc cgaggggatc acggcgtttc ggcgtctgct ggaccgcctc
      121 ttcgacgaca tcgtcccgat gctcacccgt tttccggaca gcggacgacc gctgctccac
      181 catcccgtcc gatcccgtga ggcgcggacg ctcgtgcgaa aacttcgggc gctgatatca
      241 gcgcccgacg aggtccggga gtttgtcctc gatgattacc tcctgctgta tcttcgtcgc
      301 ggacggacgc tcgtttttct ggcgatcaaa catcaccgcc aattgtcctt tgacctgcga
      361 aggttctgga cgtgacgcgg tcggggacgg tcccaactaa acaagtaatt tgagggtagc
      421 gggcggggtc gtgttgagtt gaccgagaat cgggcggctt tggaatcatt ggcggatggc
      481 atcgcatctc gctcacactc ggatgaacga cttgccgcga atctcgttga cccgagccac
      541 ccgttgttct agactacccc cactcagacg acctcgcctc atccgcccga tgatccgcac
      601 acgtcgcaca ccgagacccc gtgtcccccg acggggacgg tcccaattaa acaagtaatt
      661 gggagccagc tcgttttcca gttgaagtcc cggacgcttc ccgtcccagc ttttccaccc
      721 gcagcccgat caatcggacc agtttccgtc gcggattttc acgccgatcc aggaacggaa
      781 gaaacaattt catcgcctcc acctccaggt ccttccccga ggttgtcggc cgcggcaggg
      841 tgtgtgagcg ggacttggtt tcaaagtccg cgaagcggac cttgatgacc acggtgcgaa
      901 aggacgcgta gccggcctct gtcagccgcg ccaccacgtc acgggccatg gccttgaggt
      961 gctccatcag catccgagca tccagcgtgt cgcgcaggaa cgtttcctct tcgccgatgg
     1021 atttgacctc gtcggattcc tgaaccggat cctcgtcgcg cccgcgaacc cgctcatgca
     1081 gcgccctccc ccacttgccg aaccgctcga ccagctctgc ttccgagaaa cgcttgaggt
     1141 cttggacctt ggtgatgccc tgctgccgca gcgtgatctc ggtcttgggc ccgatccccg
     1201 ggatctttcg taccgagagc ggggcgagga aggcctcggc gtccgtgtct tcgaccacgg
     1261 tcaagccgtc gggcttgcgc gcgtcggagg cgatcttggc gatcaacttg ttgggaccga
     1321 tgccgatgga cgcggtcaaa tgttcttccg tggcgatgtc gcgcttgatc gcgatggcga
     1381 tcgcttgcgc gcgctcgtac gtgccggccg tgctcaggtc gatgtacatc tcgtcgatgc
     1441 cggcttgttc gatgctggac gcgtgccgct ccacgatggc gcggatgcgg cccgacgtct
     1501 cccggtactg ttcgaagtgg ggacggagaa acaccaccgc tggtttgccc tggcggcgcg
     1561 cccactccga cctacgccag gcctgtgtga tgggcatggc ggaatgcagg ccgtagacgc
     1621 gggccgggta gttggcggtg gcaaccacgc cgcgcccctt gccgtccatg ggatcagccc
     1681 ccaccgcaat gggcaatccc ttgaggtgcg gacgctcccg ctcttcgacc gcggcaaaaa
     1741 acgcgtccat gtccagatgc gcgatgattc gtggcggcat gatcggaccc ggaacagccc
     1801 tgatcataac gcctccaccg ccacgccggc cacggtcgtt cggagccatc gcaccacacg
     1861 cagcggcctc gggggcagga gtgacctgtt gcctttgacc gtgagtcgga tgggctgatt
     1921 gggacgatcg accggcaacc aagggttccg cgaacggtct gttcccctag ccggttccct
     1981 cactccatcc cgtgaaccgc gctccgaaca ccaggaccgc ctgacgttcc gcctgttcca
     2041 gatttccgaa ccagtactcc gccagcggcg gttcatccgg ggcgggcaac aactggaggc
     2101 agtacgcgtc cacctcggga tcgtggcgca tcgcgaggtc agatacctcg gcaagcgggt
     2161 ctcccggcgc gaggcgcgcg gtcttgagca gacggttctc cacggtcagc gggatcgagc
     2221 gcgacgagcc ggcacgaatc cgtccccgat gcggttccgc ggcttgcact ttccgatcgg
     2281 cttcgctact gtcctgatca cacggctctt cctgaatgcg aaatggcggt gtcttggccg
     2341 acacccgtgt accacatcag gcgccgttgc tcaaatctcg accacgtctc ggacagcctc
     2401 cgcccggatg gctgggcgtc atgtccggcg ggacttcgtt cagagacgat caccacgaga
     2461 gtccacccat gatgaaaaag atccgcaaca cgatgctcac cgggctcatc gcgctgttac
     2521 cgctctatct ctcgttcgtg ctgctcgtgt ggttgttcaa aaccctggac gccctctctc
     2581 agccgtggtt ggcccgcgtg ttccacgtca ccgtcccggg gctcggcatt gcggccaccg
     2641 cgttggtggt cttcgtcacc gggttgatcg tgtccagcgt ttcaggagcc ctggccctca
     2701 gttgggttga ccgcctcttc gagcgggttc ccttggtgaa ggggctgtac agcggcatca
     2761 ggcaaatcat cgaaagcttc aatcccgcca atccgtccgg attcaaggaa ttcgtgctgg
     2821 tcgagcaacc ccagagcagg gacttacacg cggggttcct gaccggggag ttcgcgctca
     2881 tcaagccgga cggcacgcgc cgcgagttcg caacggtcta tgtgcccagc aaccacctgt
     2941 acctggggac gatccagatc gtggagcgga cccggatcat tcgaacgtcc atgacgctcc
     3001 aggacggcat tacgttcacg ctttccgccg gcgcgtccgc aaaaggcgcg ttccgtgaat
     3061 cggacgccgc tgcccggtga gccaggcggg gacgttcc
//
LOCUS       DBNGTT010000040         2379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_518639, whole genome shotgun sequence.
ACCESSION   DBNGTT010000040 DBNGTT010000000
VERSION     DBNGTT010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_518639"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1147)
                     /locus_tag="AC16BC_00905"
     CDS             complement(128..1147)
                     /locus_tag="AC16BC_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240198.1"
                     /translation="MKDLMWWNVGKRERVRGLVVLLSARAFAAVACSGAAYAASDDAQ
                     SAFEQGTELNKARRFDEAIQQFSKAVELSPESHKYHQALFMTYLTTRRGRQGLEAYKK
                     MAREHPNSPRVRYWLGRIYLQSEALEDAAAAFRQATTLDPKDEHPWLSLGHVYARLGK
                     DDDAMRAYQEANRLSPKIAIVHAGLGALYLKKKDDAKALRELNEALRLDPSLSDARYS
                     LSLLYERKGELDRAMIEWQRLLDDDPNESNARERLARAYVQKKRYEEAVQEYSMLSQV
                     RQASPEVFFALGESQVLLAASLDDPRDRADLVAAARESFQRVLELDPKNADATSYLER
                     LKPTH"
     gene            complement(1302..2177)
                     /locus_tag="AC16BC_00910"
     CDS             complement(1302..2177)
                     /locus_tag="AC16BC_00910"
                     /inference="COORDINATES: protein motif:HMM:NF026037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3240199.1"
                     /translation="MQGGRGAGNGRLLAVAGLLVGTLLANPSFAYQEIVVQDGGSVTG
                     RVQLNGTPPPARIFHLVFSPNMAFCGKISDGAGNRLLREFRVAKDGAFEGVVVAVVGV
                     EKGKRFDYTPTLDIENCRISPLIMPVRNGRPLKMINKDDITHDVQAYSMRDDFAIQMF
                     NKPMIPNMRAEKTVRMRKGHYVFHTQCGVHDFMQSWGMAVGNPYFAVTAADGTFTIPD
                     LPPGQYDVLAWHPHLPVQSAQVTVSAKDRAAVDFMFEAKDVDIPLHDLQERYRLDTAL
                     DARPMPEPTLERQVQ"
     gene            complement(2200..>2379)
                     /locus_tag="AC16BC_00915"
     CDS             complement(2200..>2379)
                     /locus_tag="AC16BC_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240200.1"
                     /translation="AVIQREVTVAANGTTSIAFEFDGNSVVRPIYETQEKFRIGPEAH
                     PHEHLESCEEPYCTH"
BASE COUNT          419 a          785 c          710 g          465 t
ORIGIN      
        1 tccccccttt tccaaagggg ggagcggcaa agaaagggtt cccaggccgt tgcgcgttga
       61 gcgtcggcgc acccgtgccc aatcaaaatc ccccctttga aggggaaggc gggagttgcc
      121 gctgatctta gtgcgtcggc ttgagccgtt ccaagtagga tgtcgcgtcc gcgttcttcg
      181 gatccagttc cagcacgcgc tgaaacgact cgcgcgccgc agccaccaaa tcagcgcggt
      241 cacgcggatc atcgagcgag gcggccagaa ggacctgcga ctccccgagc gcgaagaaca
      301 cctcgggaga agcctgtctg acttgggaga gcatcgagta ttcctggacc gcctcctcgt
      361 atcgcttttt ctgcacgtat gcgcgcgcca ggcgctcgcg ggcattggac tcgttcggat
      421 cgtcgtcgag caaccgttgc cattcgatca tcgcccgatc gagttcgccc ttgcgctcgt
      481 agagcaggct gagcgagtac cgagcgtcgc tgagcgatgg atcgagccgc aaggcttcat
      541 tcagttcccg tagcgccttg gcgtcgtcct tctttttgag atacaacgcg ccaagccccg
      601 cgtgcacgat cgcgatcttg ggcgacaagc gattcgcttc ctgatacgcg cgcatcgcgt
      661 cgtcgtcctt gcccaaacgc gcgtacacgt ggcccaatga gagccaggga tgctcgtctt
      721 ttggatcgag cgtggtggcc tggcgaaatg cggcggccgc gtcttcgagc gcctcgctct
      781 gcaaatagat gcgcccaagc caatatcgaa cccgagggct gttcgggtgc tcgcgggcca
      841 tcttcttgta ggcctcaagg ccctgacgtc cgcgtcgcgt cgtgaggtac gtcatgaaca
      901 gggcctggtg gtatttgtgg ctctccggac tcaactccac cgccttggag aactgctgga
      961 tcgcctcgtc gaaccggcgg gccttgttga gttcggtgcc ctgctcgaac gcggattggg
     1021 cgtcgtcgga cgcggcatac gctgcgccgg agcacgccac ggccgcgaac gcccgcgcgc
     1081 tcaacagaac aacaaggccg cgaacacgtt cgcgtttgcc gacgttccac cacatcagat
     1141 ccttcatgct ctcccccaaa acacggccgg ggacacccat gccgttgcag gtgcccccgg
     1201 tgtctccatg ctcagtgatc cgaaatgatc gcggttatgg ccgcgcaaca atcggtcgcc
     1261 acggcaaacc cgccgtctca gccgggccga cggctacagc ctcattgcac ttgacgctca
     1321 agggtgggct ccggcatcgg ccgcgcgtcc aaggcggtgt cgagccgata ccgttcttgc
     1381 agatcgtgca acgggatgtc cacgtccttg gcctcgaaca tgaagtccac ggcggcgcgg
     1441 tccttcgcgg acacggtgac ttgggccgat tgcactggaa gatgcggatg ccaggcgagg
     1501 acgtcgtact gcccgggcgg caggtcggga atcgtgaacg tcccgtctgc cgccgtgacc
     1561 gcgaaatacg gattgccgac cgccattccc cacgactgca tgaagtcgtg cactccgcac
     1621 tgggtgtgaa agacgtagtg gccctttcgc atccggacgg tcttctccgc gcgcatgttc
     1681 ggaatcatgg gtttgttgaa catctggatc gcgaagtcgt cgcgcatcga atacgcctgg
     1741 acgtcgtgcg tgatgtcgtc cttattgatc atcttcaacg gccggccgtt gcgaaccggc
     1801 atgatcaagg gagagatccg gcagttctca atgtcgagcg tgggcgtgta atcgaagcgt
     1861 ttgcccttct ccaccccgac caccgccacc accacgccct cgaacgcgcc gtctttcgca
     1921 acccgaaact ctcggagcaa ccgattcccc gccccgtccg agatcttccc gcagaacgcc
     1981 atgttcggcg aaaagaccag atggaagatt ctggcgggcg gcggcgtccc gttgagctgc
     2041 acccgccccg tcaccgagcc gccatcctgc accacgatct cttgatacgc gaatgacggg
     2101 ttcgccaaca gcgttccaac gagcagaccg gccaccgcga gcaggcgccc gtttcccgct
     2161 cctctccctc cctgcatcac gtgcgactcc tcgttcccgc taatgggtgc agtacggctc
     2221 ttcgcagctc tccaggtgtt cgtgcggatg cgcctcgggc ccgatgcgga acttctcctg
     2281 ggtctcgtag atcggacgca ccacggaatt gccgtcgaat tcaaaggcga tgctcgtggt
     2341 cccgttggcg gcgaccgtca cttcccgttg gatcaccgc
//
LOCUS       DBNGTT010000041         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_135821, whole genome shotgun sequence.
ACCESSION   DBNGTT010000041 DBNGTT010000000
VERSION     DBNGTT010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_135821"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="AC16BC_00920"
     CDS             complement(<1..318)
                     /locus_tag="AC16BC_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR3240201.1"
                     /translation="MAFTLTSPVIGPGQVIPKKYTCDGPDVSLPLAWSDPPAGTRSLA
                     LIADDPDAPVGDWVHWVLYDLPAHVRELPEGVPRDNTLPDGSKQGKNDFGRLGYGGPC
                     PPPG"
     gene            516..752
                     /locus_tag="AC16BC_00925"
     CDS             516..752
                     /locus_tag="AC16BC_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transport protein"
                     /protein_id="HIR3240202.1"
                     /translation="MDPLSLFSLDPWTLFLGLVFSVIGFGYYRYGRRQSETGALIAGI
                     ALMVYPLFVTSPATVAVIGLLLMAGPVVAKRFPR"
     gene            850..2241
                     /gene="sthA"
                     /locus_tag="AC16BC_00930"
     CDS             850..2241
                     /gene="sthA"
                     /locus_tag="AC16BC_00930"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HIR3240203.1"
                     /translation="MSRRYDLVVIGAGPAGQKGAVQAAKLKKSVAIVEKEPHPGGICL
                     YTGTLPSKTLRETVMFYQGFRKRLLRGLSCQLDKAMTLADLMYQKDKVIEHERAIISD
                     QLMRNDVRVIRGRAAFEDAHRIAVADPEGGRTELQAEYVLITTGSRPHHPPCGPRVEA
                     PIFDSDTILKISRIPRTLTVLGGGVVGCEYACIFSALGTKVTLVDRRNELMRFMDEEI
                     AAALSFHMRDMGTTFRLGEDVESVEVVDAEQVCTRLKSGKIIYSEALLSAAGRAAATN
                     GLNLSNIGVPEQMSGHIEVNEHYQTAVPHVYAAGDVIGFPSLASASMDQGRLAVCHMF
                     QYPASSFPHVLPYGVYTIPEISMVGKTERELTDECVPYETGVARLREIARGQIIGETE
                     GLLKLIFHQHTRELLGVHVIGEGATELVHIGQAVIAHKGTIEYFVHSVFNYPTLAEAY
                     KVAALNGLNKLRV"
     gene            complement(2288..2725)
                     /locus_tag="AC16BC_00935"
     CDS             complement(2288..2725)
                     /locus_tag="AC16BC_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HIR3240204.1"
                     /translation="MEEFRSKLEPVHRQRPRRVEEIQKEYAEMMDRLREEEERGERMH
                     ELLEHTLRDIAQRIGGEIPSGDLDAYYRFVAHQSAELTHLRDTLDQLRADCEAKRAVL
                     VGATHEEPAIAEPERSHQRAERLALDAREQYAVDDLASRRSAA"
BASE COUNT          467 a          900 c          968 g          517 t
ORIGIN      
        1 gccgggcggc ggacacgggc cgccgtagcc gaggcggccg aagtcgttct tgccctgctt
       61 ggagccgtcg gggagcgtgt tgtcgcgggg cacgccctcc ggaagttcac ggacgtgggc
      121 gggcaaatcg tacaacaccc aatgcaccca atcgccgacc ggggcgtcgg ggtcgtcggc
      181 gatcaacgcc aggctccgag tcccggcggg cggatcgctc cacgccagtg ggagcgacac
      241 gtcggggccg tcgcacgtat atttcttcgg gatgacctgt ccaggaccga tcaccggact
      301 cgtgagtgta aacgccatgg cgttccctcc gtgggtggtc gcgattcgtt cgtcgtcgcg
      361 ctggaacgca ctattgtagg cgctgttccg gggcacgcgt caacccgtag ccgccatcag
      421 gtcgcgccgc gtgttcagga ggcggccggc acgtcgatgc gcttgccgac gcggggcgtt
      481 tgtcgtatcg tgatcgctga gacgtgggag gcgagatgga tccgctgtcg ctcttttcgc
      541 tcgacccgtg gaccttgttt ctggggctcg tgttcagcgt gatcggcttc gggtattacc
      601 gctacggcag acgccagagc gagaccggcg cgttgatcgc gggcatcgcg ctgatggtct
      661 atccgctttt cgtgacgagt cccgccaccg tcgccgtcat cggcttattg ctcatggccg
      721 gcccagtggt ggccaaacgc ttcccgcggt agccccccca gacgttcttc cggcgtttca
      781 tcgcgacgcg tccgcggcac gatgtgaatc cgatgctata ctcctcagcg gggcgtgggg
      841 aggtcaggga tgtctcggcg gtatgatctg gtcgtgatcg gggcggggcc tgcgggtcaa
      901 aagggcgcgg tccaggccgc caaactcaag aaatcggtcg cgatcgtgga gaaggagccc
      961 caccccgggg gcatctgtct ctacaccggc acgctgccca gcaaaacctt gcgcgagacc
     1021 gtgatgttct accaggggtt ccgcaaacgc ctgctgcgcg gcctgtcctg ccagctcgac
     1081 aaggccatga ccctggccga tctcatgtac cagaaggaca aggtgatcga gcacgagcgc
     1141 gcgatcattt cggatcagtt gatgcgcaac gatgtgcggg tgatccgtgg gcgcgccgcg
     1201 ttcgaggacg cgcaccggat cgcggtggcc gatccggagg gcggacggac cgagctccag
     1261 gccgagtacg tgttgatcac taccggcagc cgaccgcacc accccccgtg cggtccgcgc
     1321 gtcgaggcgc cgattttcga cagcgacacg attctcaaga tcagccggat tccccggacg
     1381 ctcacggtcc tgggcggcgg ggtcgtcggg tgcgaatacg cgtgcatttt ctccgcgctg
     1441 ggcaccaagg tgactctggt ggatcgccgc aacgagctca tgcggttcat ggatgaggag
     1501 atcgccgcgg cgttgagctt ccatatgcgg gacatgggca ccacgttcag gctgggggaa
     1561 gacgtggagt cggtcgaggt ggtggacgcg gaacaggtgt gcacgcgcct caaaagcggc
     1621 aagatcattt acagcgaggc cctgctgagc gccgcgggcc gagcggccgc caccaacggc
     1681 ctgaatctgt cgaacatcgg ggtccccgag caaatgagcg ggcacatcga ggtcaacgag
     1741 cactaccaaa ccgcggtgcc gcacgtctat gcggcgggcg acgtgatcgg attcccgagc
     1801 ctggcgtcgg cgtccatgga ccaagggcgc ttggccgtgt gccacatgtt ccagtacccg
     1861 gcgagctcgt ttccccatgt actgccgtac ggcgtgtaca cgattccgga gatttccatg
     1921 gtcgggaaga ccgagcggga actgaccgat gagtgcgtgc cgtacgagac cggggtggct
     1981 cgcttgcgcg agatcgcccg cgggcagatc atcggggaga ccgagggcct gctcaaattg
     2041 atcttccacc aacacacgcg cgaactgctc ggggtgcacg tgatcgggga gggcgcgacc
     2101 gaactggtcc acatcgggca ggccgtgatc gcccacaaag gcacgatcga atacttcgtg
     2161 cacagcgtgt tcaactaccc gactctggcc gaggcctaca aggtcgcggc gctcaacggc
     2221 ctcaacaagc tgcgggtgta acgcggacca gccgcgcgtc ccggccggtc aggatgtcgg
     2281 cggcgcttca cgcggccgat cggcgagagg ccaggtcgtc gaccgcgtac tgttcgcgcg
     2341 cgtcgagcgc gaggcgctcg gcccgttgat gggagcgctc cggctccgcg atcgccggtt
     2401 cctcgtgtgt ggcgccgacc aggaccgcgc gcttggcctc gcaatcggcc cggagctggt
     2461 cgagcgtatc acgcaggtgg gtcaattccg ccgactgatg ggccacgaac cggtagtacg
     2521 cgtcgagatc tccggagggg atctcgccgc cgattcgttg cgcgatgtcc cgcagcgtat
     2581 gctccaacaa ctcgtgcatc cgctccccgc gctcctcttc ctcgcgaagc cggtccatca
     2641 tctccgcgta ttctttctgg atctcttcga cgcggcgcgg ccgctgccga tggaccggtt
     2701 cgagtttcga tcggaactcc tccatggggg tacctccgtg accggttggc ccgacgtacc
     2761 ggcctcaggt ctgcggtagc cgggtcaatt ccgcacgcta cggttgatag agcaatagtg
     2821 gggccaatgc gtttttcggc tttcgaggca ct
//
LOCUS       DBNGTT010000042        12857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3202062, whole genome shotgun sequence.
ACCESSION   DBNGTT010000042 DBNGTT010000000
VERSION     DBNGTT010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3202062"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1247)
                     /locus_tag="AC16BC_00940"
     CDS             complement(78..1247)
                     /locus_tag="AC16BC_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3240205.1"
                     /translation="MTPIYLDHIAAAKPHPEVVAAMLPWLTEDFGNPMSLHWFGEKPR
                     AAVEEARSQVADLLGVDPREVTFTGSGTEANTLALRGLMAPLARRGRHLIVSAIEHPS
                     ILDTARRLERDGAAALTVVPVDRYGRVDPGAVSSAIRDDTVLISIQTANPEIGTVQPV
                     AELARVARQRGVLIHTDAVVAVGRTPLDVRELDVDAVSLAANPFYGPPGVGALYVRRG
                     VRLEPLLVGGAQEAGRRAGGHNVPAIVGMGRAAVLVNEYGATWRRDMAALHQQLSEGL
                     SGVAGALMTGHPDHRLAGHVSACFEYVEGEALVRMLGQAGVAVASGSACSDFTVSSKV
                     SHVLSALGIEPSRAQGSVVCSLGRETTETQIREVARLVPRTIEGLRRLSPLYQRG"
     gene            1458..1775
                     /locus_tag="AC16BC_00945"
     CDS             1458..1775
                     /locus_tag="AC16BC_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3240206.1"
                     /translation="MSPSHGKLGLMLSTGPEHANLDTVVRISDAARRRGNDVYLYLID
                     EGTRCLDTAPVRRLPGTGVKLFVCAFGAQKAGVPIDDRATFCGLVVLSDIITGCDRFL
                     AFN"
     gene            1775..2128
                     /locus_tag="AC16BC_00950"
     CDS             1775..2128
                     /locus_tag="AC16BC_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3240207.1"
                     /translation="MAARRIAVVITGDPMTSARPGEAIRIALGLGSGTHTVSVVLRGP
                     AAGLLFPEAEDSACADEVDKYLPSLGETLEPGFYVEQAALTGRAVDESDYRIIPVDVP
                     GIGNVLAQADRFLVF"
     gene            2134..2391
                     /locus_tag="AC16BC_00955"
     CDS             2134..2391
                     /locus_tag="AC16BC_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240208.1"
                     /translation="MGHTLHIVKSVGSPHPWDLIAQHASTDEYMIVLIQDAVAARPPL
                     AGPTFVLERDARARGASTTYPLIDDDRLVDLIWEADTVVVW"
     gene            complement(2395..2790)
                     /locus_tag="AC16BC_00960"
     CDS             complement(2395..2790)
                     /locus_tag="AC16BC_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240209.1"
                     /translation="MVAAGLAAAAMTALGWWLAAPDPQTHGVLLSVGPSAKSDPYGVA
                     QGAVTHGEAVLIDFGDGEFAFFVNRAAEVRALVNGCGDRVGPDRQTRLPVLVEAHFQI
                     RNVPPYVVEVRGARYRFEAPSGVGRQTCG"
     gene            complement(2818..3636)
                     /locus_tag="AC16BC_00965"
     CDS             complement(2818..3636)
                     /locus_tag="AC16BC_00965"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR3240210.1"
                     /translation="MIRARGHAPRPTRGSAALWNEERIVPPPLERLPGSRVLVLAPHM
                     DDETLGCGGSLYRHALAGDAVTVVYMTDGRKGDPALNARELGSAERERLEDDLAATRR
                     EEARRSATVLGVRDLRFLENRDQELRVSPRTRRQVRELLEALRPDLVCLPFPTDHHPD
                     HQATNRIFLSALSAARGIEPPLCAGYEVWAPLEPNCLVDITAVAEIKQRALAEFASQM
                     AVIDYGRCIMGLHAYRSITHLHGRGFAEAFVLLPAAAYRREVTRRLRGPRGRSR"
     gene            3818..4225
                     /locus_tag="AC16BC_00970"
     CDS             3818..4225
                     /locus_tag="AC16BC_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3240211.1"
                     /translation="MGGTNPYIERPKIELPHKAYKITFVNKGVTLDVDPAKIPYGHTG
                     QPGSILDIALASGVEIEHVCGGVCACSTCHVIVKQGLESCNEATDDELDQLDEAPATT
                     LQSRLACQTVPNGSMDLVVEIPEWNKNLAKEDH"
     gene            complement(4240..4929)
                     /locus_tag="AC16BC_00975"
     CDS             complement(4240..4929)
                     /locus_tag="AC16BC_00975"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240212.1"
                     /translation="MADALISVEGVRKTFHRGGFAVQVLDGIDLTIERGAFLALMGPS
                     GSGKTTLLNLIAGIDRPTAGRVVVDGTDITGLSERDLTAWRARHVGFIFQLYHLIPVL
                     TALQNVELPLTLTHLSRRDRREHALTALKVVGLSDRADHYPRQLSGGQEQRVAIARAI
                     VTDPTLIVADEPTGDLDAQSAEEILTLLGRLHKEFQKTIVMVTHDPHAAARAEVLVRL
                     EKGVLTSARAG"
     gene            complement(4933..5166)
                     /locus_tag="AC16BC_00980"
     CDS             complement(4933..5166)
                     /locus_tag="AC16BC_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240213.1"
                     /translation="MFIKREDYQYLEGLCKSLSAPQQERLMTALLGKDRGGSLGERVR
                     IVGEEIYDSQGVEQKDWITRTMIKKAFKDAKQG"
     gene            complement(5181..8492)
                     /locus_tag="AC16BC_00985"
     CDS             complement(5181..8492)
                     /locus_tag="AC16BC_00985"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3240214.1"
                     /translation="MTPPIDAFERERAIRTFDANVVVTAGAGTGKTTLLVERLVHLVV
                     REPDPVPVTEIVALTFTNKAAAELVVRFRERIEALARAAERPEEEGGLRDAFAALVTR
                     TGASADTVGERLHTALANLDRSHIGTIHSFAATLLRLFPIEAGVDPKFREADEAARRD
                     HVEEAWRAWLDHELAREAPRAALWRRLLERLTLDDLFELAVGLSAELIPLDALGRLTA
                     APPQVGEWLVGACGAASRLLATHPQTRVIEQRLAAASRVIACAAEGRAAEETDCAAVA
                     ADVTSVPKGWDERDADRAKGLIKLARRLSAVDAETVRLIGDTLAPFVRECRASLARAG
                     RLPFDGLLARARDLVRDHLAVRETLKRRFRALLVDECQDTDPAQYEIMVYLSEALGGR
                     GAHWRSVRLARGKLFLVGDPKQSIYGFRGADLEAYQDVARLVLDQGGVECRLTTNFRS
                     RGAILDAVNAVGSRLLAYRPGVQAAYEPIESTSEAMGGLKPIVRVVRARHGAFDAGAG
                     REAEAESLARWLRGSVFGVLDVRAAQGRTMRAEPGDVAILFRALSEVHVYLEAFRRHG
                     IDYVVEGERRFFAAQEVVDAINVFRALASPYDVPALVGVLRSPLGGMTDRQLYALSRA
                     GWLDYRLANTGDAPGPWAPVALYRELAALSREAPRMPVAQAVGAVFERFPLQAIAAGG
                     PHGDQSAANVEKLERIARELADEGLSFASLAARLDRRVRDEVEEGESPLVDEAVNAAK
                     VLSVHKAKGLEFPIVVLAGAHGGRRAGAWPPVANHWSSGVVGVAVGGVASLTGVYLAE
                     RMAEKDAEESRRVLYVAMTRARDLLVISGAETERGPARDGPAALVEDATGAAWGDVPR
                     ESPAKDLFEWTVVEAESDRGSAEPRSTVDLAWPDQHAFASRWAERAARAERTAQSLMF
                     VTPTRLAARRAMARPATGRRRGSADAERARRVGTLVHAFLQHWDFGTDSSAWPSALDA
                     FAGGQLDGTADVAVEDLRAALGPFFASPMYAELARATIIGREVPLLMPWEGAMMEGAI
                     DLIYEDGAGLYVADYKTDSVDAAGAPAAAERYRVQADVYTRAVRESLGRSVAAFRCLF
                     VRPGVAVDLTPGNDH"
     gene            complement(8489..11572)
                     /locus_tag="AC16BC_00990"
     CDS             complement(8489..11572)
                     /locus_tag="AC16BC_00990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3240215.1"
                     /translation="MLTITCGSYPSLETALADDVMAVKAADPLAPVIIVVPSDSLRRR
                     IVRLLAGERKRAFLSASVLTFHQLSRRLVEESIRRPLPPLVDDQAYEELMRVLLDREN
                     AGHAFAGVAATRGGGAALWQTLRDLKDAKVPPRALAEAVAESLFPAEAASRLAGLAGL
                     YGEVLSAAETAGWVDYTDLDVLAAAAAPSSGMLKTQRLVAYYGFYDLTQAQYDVLRAV
                     SGAADAAVYFPFVSGDPSWAFADRFLTRYLTGVSTRPVRFIDGPVPSRPSVTLVSCSG
                     VTDEVRACAKAILALVEDEGLAFEDIGVVSRSLDPYADAIAREFARHHLPLVTTATRP
                     LSRHPLAQAALRLARIAEGAPAREDVIDFVASPWCRVERLAPHVHADPALWDELARSV
                     GVRHGYEAWSRLAALVGPDGGPETGSSSRGAQAAVLLQVVNGLRRACGALPETASGSS
                     HADAWRGLLASLLGIEREADASDDDPDGVDAVASAVLAAFDAPARLDVVTATMTKAAY
                     LDVVRRRIESTALPLTDPHARGVQVMDAASARGTACRALFLLGLNEGVFPRVIREDAF
                     LRDQERRLIETTLGYKIPEKLAGYEEERLLFAALTGGGGRDRVVLSTQRADDAGRALA
                     PSWYVAPWRDEASNTPRVDVPRRTREKPRVAPFARVDRLTPQEASVLAELVGVRSVAV
                     ADGRTREWLVRAASCRDAIEAWATTLSAFDGDVGTPEEWLARQREAGYAATGLATYAA
                     CPWQFFVTRVLDVSPSSDAEVDAGPTARDWGLLAHEAIARAARDSASDINAIWREVCA
                     RYARRAGIGYPLVWDLAVERIGRVVAEVAADDAGERDRSGYTVIETEVALRGELGADP
                     VVPIQGRLDRLDQAPDKGLRVIDWKFRDARGANRRNELVAEALRGRGLQPPIYEALAQ
                     RYALTRLGKAVGESAAVVYEVGLNERDAGIEREPYAPDPDMRTRILGTVRALVDGIEQ
                     GVFPMNPDAHCAWCEVAASCRRRHAPSRARAERDPRSVGLDRIRRAPVRPPKEPG"
     gene            complement(11800..12483)
                     /gene="scpB"
                     /locus_tag="AC16BC_00995"
     CDS             complement(11800..12483)
                     /gene="scpB"
                     /locus_tag="AC16BC_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3240216.1"
                     /translation="MDDRELKPIVEALLFVAAEPMPLNKLCEILDGVERERVVACLDD
                     LREQYRRGDHGLTVAEVAGGYQLVTVQEAAPWLRKLSAAKAPPRLSKPALETLAIIAY
                     KQPLTRPEVESIRGVDVAGVVKTLMDRRLVKIVGRKDVPGRPMMFGTTKEFLHAFRLK
                     DLADLPTLKDFAEIARATDASVGDQEVAEGELPASDTETSGASPDVREIVESADSPVS
                     DAPTEPSLA"
     gene            complement(12476..>12857)
                     /locus_tag="AC16BC_01000"
     CDS             complement(12476..>12857)
                     /locus_tag="AC16BC_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3240217.1"
                     /translation="EYELGELSVFDLLTALRDVLDRVPDPTIMEVTVEALTVRDRMTA
                     IIERFDALAEGERVRFHELFEGVATRTAVIVTFLALLELVKMHVLGLRQTGPNAEIDV
                     LRLESVPAEDGGEMNASQGDTLDG"
BASE COUNT         2134 a         4689 c         4098 g         1936 t
ORIGIN      
        1 gagagccccg tgttgcaccg tgccgcgttt gttggagcga gcgggaattt ctggcatcgc
       61 cactgcgatg agggtcgcta cccgcgctga tacagggggg acaaccgacg caacccttcg
      121 atcgtccggg gaaccagccg cgcgacctcg cggatctgcg tctcggtggt ttcacgcccg
      181 agcgagcaga ccacggagcc ttgggctcgg ctcggctcga tcccgagcgc ggacagcacg
      241 tgggacacct tcgaggaaac cgtgaagtcg ctgcacgctg atccgcttgc gaccgccacg
      301 cccgcctgcc ccagcatgcg cacgagcgct tcaccctcca cgtattcaaa acaggcgctg
      361 acgtgtccgg ccagacggtg gtcggggtgt ccggtcatca atgcgcccgc caccccggag
      421 agcccctcgg acagttgttg gtgcagcgcc gccatgtcgc gccgccacgt cgcgccgtat
      481 tcgttgacca ggacggccgc gcgccccatg cccacgatgg ctggaacatt gtggcccccg
      541 gcgcggcggc ccgcttcctg cgcgccgccg accagcagcg gttcaagccg gaccccccga
      601 cgcacataca gcgcgccgac gccgggcggg ccgtagaacg gattggcggc gagactgacc
      661 gcgtccacgt cgagttcgcg aacgtcgaga ggcgttctcc ccaccgccac caccgcgtcc
      721 gtatgaatga ggacgccccg ttgccgcgcg acgcgcgcaa gctcggccac cggttgcacg
      781 gtgccgattt ccggattcgc ggtctggatc gaaatcaaca ccgtgtcgtc gcgaatggcg
      841 gatgagactg ctccgggatc gacgcgaccg tatcggtcca ccggtacgac ggtcagcgcg
      901 gccgcgccgt cccgctcaag ccggcgcgcc gtatccagga tggacgggtg ttcgatcgcg
      961 gacacgatca gatgccggcc gcgccgcgca agcggcgcca tgaggccgcg caacgcgagc
     1021 gtgttggcct cggtcccgct gcccgtaaac gtcacctccc gcggatcgac ccccaacagg
     1081 tccgcgacct gactgcgcgc ctcctccacc gccgcgcggg gcttctcccc gaaccaatgc
     1141 agcgacatcg gattgccgaa gtcttccgtc agccacggga gcatcgcggc aacgacctcg
     1201 gggtgcggtt tggcggccgc gatgtgatcc agatagatcg gcgtcatggt ggccggcatt
     1261 gtatcacgcc ccgtcgcacc gcacgagagg gtcccctctt gagcggacac tgccgcgtga
     1321 gcctgacctc gttcgggtgc gacccatcac ccgtcgtcca cgaaacgcga tccgacgatc
     1381 cgcgtcatgg ccgtaaggca tccatgaact cgcggcgttc attgacgcgc ggcgcgggag
     1441 ggtgctagca tcgcgcggtg tcacccagtc acggcaaatt ggggctgatg ctctccaccg
     1501 gccccgaaca tgcaaatctc gacaccgtgg tgcgaatctc ggacgcggcg cgccgccgtg
     1561 gcaacgacgt ctacctctac ttgatcgacg agggcacgcg gtgcctggac acggcccctg
     1621 ttcggcggct tcccggcacc ggcgtcaagc tctttgtgtg cgcgttcggg gcgcagaagg
     1681 cgggcgtgcc gatcgatgat cgcgccacgt tttgcggcct cgtcgtgctg tcggacatca
     1741 tcaccggctg cgaccggttt ctcgcgttca actgatggcc gcgcgacgaa tcgcggtcgt
     1801 catcacgggc gaccccatga cgagcgcccg ccccggcgaa gccattcgca tcgcgttggg
     1861 cctcggcagc ggaacgcaca cggtctcggt cgtgttgcgc ggccctgccg cgggcctctt
     1921 gttcccggag gccgaggact cggcgtgcgc ggacgaggtc gacaagtatc tcccgtcatt
     1981 gggcgagacg ctggaaccgg gattctacgt ggagcaggcg gcgctcacgg gccgcgcggt
     2041 ggacgagagc gactaccgca tcatcccggt tgacgtgccc ggcatcggaa acgtgctggc
     2101 gcaagccgac cggtttctgg tgttctgacg accgtgggcc acacgctgca catcgtcaag
     2161 tccgttggat cgccacaccc ctgggatctg atcgcgcagc acgcctcgac ggacgagtac
     2221 atgatcgtat tgatccagga cgcggtcgcg gcgcgcccgc cgttggccgg tccgaccttc
     2281 gtactggaac gcgacgcgcg ggcacgcggc gcgtcaacga cctatcccct gattgatgac
     2341 gatcggctgg tggacctgat ctgggaagcc gacaccgttg tggtctggta gtccctaccc
     2401 gcacgtctga cggccgacac cggagggcgc ctcgaaccga taccgcgccc cgcggacctc
     2461 gaccacgtac ggcggcacat tgcggatctg aaagtgcgcc tccacgagaa ccgggagtcg
     2521 ggtctggcga tccggcccca cccggtcccc gcagccattc acgagcgccc gcacctcggc
     2581 ggcgcggttg acgaaaaacg cgaactcgcc gtccccgaag tcgatgagca cggcctcccc
     2641 gtgcgtgacc gcgccctgcg ccaccccgta gggatccgat ttcgccgaag gtcccaccga
     2701 caggagcacc ccgtgcgttt gcggatcagg cgcggcgagc caccaaccga gcgcagtcat
     2761 ggcggccgcc gcgagtccgg ccgcgaccat ccgtcggagc gttctcgacg acacccgtca
     2821 gcgagaccga ccgcggggcc cgcgcaaccg gcgggtgacc tcgcggcggt aggcggcggc
     2881 cggcagcagg acgaacgcct cggcgaatcc gcggccgtgc aagtgggtga tcgaccggta
     2941 cgcgtgcaac cccatgatgc agcggccgta gtcgatgacg gccatctggc tcgcgaactc
     3001 cgccagcgcc cgttgcttga tctccgcgac cgccgtgatg tccaccagac aattcggttc
     3061 gagcggcgcc cacacttcat agccggcgca cagcggcggc tcgatgcccc gcgccgcgga
     3121 cagggccgac aagaagatgc ggttcgttgc ctggtggtcg gggtggtgat cggtgggaaa
     3181 cgggaggcag accagatccg gcctcagcgc ttccagcagc tcgcggactt gccgccgcgt
     3241 cctcggcgac acccgtaact cctggtcgcg gttctccaga aagcgcaggt cacgcacgcc
     3301 gagcaccgtc gcgctgcgcc gcgcttcctc gcggcgtgtc gccgccaggt cgtcttcgag
     3361 acgctcccgc tcggccgacc ccagctcccg ggcgttcagc gcgggatcgc ccttgcggcc
     3421 gtcggtcata tagacgaccg tcaccgcgtc gccggccagc gcgtggcggt agaggctgcc
     3481 cccgcagccc agggtttcat catccatatg cggtgccagc accagcactc gtgaccccgg
     3541 gagtcgctcc aacggcgggg ggacgatccg ttcctcgttc cacaacgccg cggatccccg
     3601 cgtgggacgc ggcgcgtgcc cgcgcgctcg gatcaccgtc tgcctccgtt gtcgtgtcat
     3661 cccggctctc ccgccatgtt ccgaccgtcg aggggaagta taaaccactc catcacatcc
     3721 ttgcgacgca cgttcagggc gggtattcac cttggctggg gcaagcccca tgtggtagat
     3781 tggaccgctt gacgacacca ccattcagga gggcggcatg ggcggcacca acccctatat
     3841 cgagcggccg aaaatcgagc tgccacacaa ggcgtacaag atcacgttcg tgaacaaggg
     3901 cgtcacgctg gacgtagatc cggcgaaaat tccctacggg cacacgggcc aaccgggcag
     3961 catcctggac atcgccctgg catcgggcgt ggagatcgaa cacgtgtgcg ggggcgtgtg
     4021 cgcgtgctcg acctgtcacg tcatcgtgaa acaggggctg gaatcctgca acgaagccac
     4081 ggatgacgag ctggaccaac tcgacgaagc gcccgccacc accctgcagt cacgcctggc
     4141 gtgccagacc gttcccaacg ggagcatgga cctggtggtc gaaatcccgg agtggaacaa
     4201 gaatctggcc aaggaagacc actaaccgcg ccgacggccc tatccggcgc gcgccgacgt
     4261 caacacgcct ttttccaacc ggaccagcac ttccgcccgc gccgccgcgt gagggtcgtg
     4321 ggtgaccatc acgatggtct tctgaaactc cttgtggagc cgccccagca aggtcaaaat
     4381 ttcctcggcc gattgcgcgt caagatctcc ggtaggctcg tccgccacga tcaacgtggg
     4441 atcggtcacg atcgcccgag cgatcgccac gcgctgctct tgccccccgg agagttggcg
     4501 cgggtaatgg tcggcgcggt ccgagagccc caccactttg agcgcggtca acgcgtgttc
     4561 ccggcggtcg cgccgcgaca aatgcgtcaa cgtcagcggc aactcgacgt tctggagcgc
     4621 ggtgagcacg gggatcaggt ggtacaactg gaaaatgaac ccgacgtggc gcgcgcgcca
     4681 cgcggtgaga tcgcgttcgg agagtccggt gatatcggtg ccgtcgacca cgacccgccc
     4741 cgcggtgggc cggtcgatgc ccgcgatcag attcagcaac gtggtcttcc cggagccgga
     4801 cggccccatg agcgcgagga atgcgccccg ctcgatggtc agatcaatgc cgtccagcac
     4861 ctggaccgcg aagccgccgc ggtgaaaggt ttttcgaacg ccctcgaccg agatcaacgc
     4921 gtccgccatt cgttatccct gcttggcgtc cttgaacgcc ttcttgatca tggtccgcgt
     4981 gatccagtct ttttgctcca cgccctggga gtcgtagatc tcctcgccca cgatccggac
     5041 ccgctccccc aacgacccgc cgcggtcctt gccgagcaaa gcggtcatca gccgttcctg
     5101 ctggggcgcg gacaacgatt tgcagagccc ctccaagtac tggtagtcct cgcgcttgat
     5161 gaacatcgcc ccctcgctga tcagtggtcg ttgcccggcg tcagatccac cgccaccccg
     5221 ggccgcacga acaggcaccg aaacgccgcc acgctccgcc ccagactctc tcgcaccgcg
     5281 cgcgtgtaca cgtcggcctg gacgcggtag cgttccgcgg cggccggcgc gcccgcggca
     5341 tcgaccgaat cggtcttgta gtccgccacg tagagccccg cgccgtcttc atagatcagg
     5401 tcgatcgcgc cctccatcat cgcaccttcc cacggcataa gcaacgggac ctcgcggccg
     5461 atgatcgtgg cgcgcgcgag ttcggcgtac atcggcgacg cgaagaacgg cccgagcgcg
     5521 gcgcgaagat cctccacggc gacgtccgcg gtcccgtcga gttgcccgcc cgcgaacgcg
     5581 tccagcgcgg acggccacgc gcttgaatcc gtcccaaagt cccagtgctg caagaacgcg
     5641 tgcaccaacg tgccgacgcg ccgcgcgcgt tcggcatcgg ctgatccgcg ccgtcgccca
     5701 gtggcgggcc gagccatggc gcgtcgcgca gccagcctgg tgggggtcac gaacatcagc
     5761 gattgcgcgg tccgttccgc gcgcgccgcg cgctcggccc atcgcgacgc gaacgcgtgt
     5821 tgatccggcc acgcaaggtc gacggtcgat cggggctcgg ctgatccgcg atccgactcg
     5881 gcttccacca cggtccactc gaacaggtcc tttgcgggcg attcgcgcgg gacgtcgccc
     5941 cacgccgcgc ccgtcgcgtc ttcgaccagc gccgcgggac cgtcccgagc cggaccgcgt
     6001 tcggtttccg cgcccgaaat gaccagcaaa tctctcgccc gcgtcattgc cacgtacaag
     6061 acgcgccgac tctcctccgc gtccttctcg gccatgcgct ccgccagata cacgccggtg
     6121 agcgacgcca cgccgccgac cgcgacgccg accacgccgc tgctccagtg gttcgccacc
     6181 ggcggccatg cccccgcgcg ccgcccgccg tgcgccccgg ccaggaccac gatcgggaat
     6241 tccagacctt tggccttatg cacgctgagc accttcgccg cgttcaccgc ttcatccacc
     6301 agcgggctct ccccttcttc gacttcgtcg cgcacccggc gatccaggcg cgcggccagc
     6361 gacgcgaacg acaggccttc gtcggccaat tcgcgcgcga tccgctccaa tttctcgacg
     6421 ttggccgcgg actgatcccc gtgcggaccg ccggccgcga tcgcctggag cgggaaccgc
     6481 tcgaacaccg cgccgacggc ctgggcgacc ggcatgcggg gcgcctctcg ggacaatgcc
     6541 gcgagttcac ggtagagcgc gacaggcgcc cacgggcccg gcgcatcccc ggtatttgcg
     6601 agtcggtagt cgagccatcc cgcgcgcgag agcgcgtaga gctggcggtc cgtcatgccg
     6661 cccaacggcg accggagcac gcccaccaag gccggcacgt cgtacggcga cgccagcgcg
     6721 cgaaacacgt tgatcgcgtc caccacttcc tgcgcggcga agaagcggcg ctccccctcc
     6781 accacgtagt cgataccatg acggcgaaac gcttccagat acacatggac ttcgctgagc
     6841 gcgcgaaaca agatggcgac gtcgccgggc tccgcgcgca tcgtgcgtcc ctgcgcggcg
     6901 cgaacgtcca ggacaccgaa caccgaaccc cgaagccacc gcgccaggct ctccgcctcg
     6961 gcctcgcggc ctgctccggc gtcgaacgcg ccgtggcggg cgcgcacgac ccgcacgatc
     7021 ggcttgaggc ctcccatggc ctccgacgtg gattcgatcg gttcgtacgc ggcctggacc
     7081 ccgggccgat acgccagtaa ccggctgccc accgcattga ccgcatcaag aatcgctccg
     7141 cggctccgaa aattggtggt caaccgacac tccacgccac cttgatcgag caccaaccgc
     7201 gccacatcct gatacgcctc cagatccgcg ccgcgaaatc cgtagatcga ctgtttcggg
     7261 tcaccgacga gaaacagttt cccgcgtgca agccgcacgc tccgccaatg cgcacccctg
     7321 ccgccgagcg cctccgagag atacaccatg atttcgtatt gggccgggtc cgtgtcctgg
     7381 cactcgtcca cgaggagcgc gcggaagcgg cgcttgagcg tctctctgac cgcgaggtgg
     7441 tcccgcacca agtcgcgggc ccgcgcaagc aatccgtcga acggcagccg gccggcccgc
     7501 gccagcgagg cgcggcattc acgcacgaac ggcgcgagcg tatcgccgat caaccggacg
     7561 gtctccgcgt caaccgcgga caaccggcgg gcgagcttga tgaggccctt ggcgcggtcc
     7621 gcatcgcgct cgtcccaccc cttgggaacg gaggtgacgt cggccgccac cgccgcgcaa
     7681 tccgtctcct ccgcggcgcg cccctcggcc gcgcaggcga tcacgcggga cgccgcggcc
     7741 aggcgttgct cgatgacgcg cgtttggggg tgcgtggcca acagtcgact ggccgcgccg
     7801 caggccccga cgagccattc cccgacctgg ggcggcgccg cggtcaaccg accgagcgcg
     7861 tcgagcggga tcagctccgc gctcaacccc acggccagtt caaaaaggtc gtcgagcgtc
     7921 agccgctcca gcagccgtcg ccacaacgcg gcgcgcggcg cctcgcgcgc cagttcatga
     7981 tcgagccacg cgcgccaagc ctcctccacg tggtcccggc gcgcggcttc gtcggcttcg
     8041 cggaatttcg ggtccacgcc cgcttcgatg ggaaacaacc tcagcaacgt ggccgcgaac
     8101 gagtggatcg tgccgatgtg ggagcggtcg agattggcga gcgccgtatg cagacgttcg
     8161 cccaccgtgt ccgcggacgc acccgtccgc gtaaccagcg ccgcgaacgc gtcgcgaagc
     8221 ccgccctcct cctccggacg ttcggccgct cgggcgagcg cctcgatccg ctcgcgaaac
     8281 cgcaccacca gttccgccgc ggccttgttg gtaaacgtca aggccacgat ctcggtgacc
     8341 ggaaccggat caggctcacg aaccaccagg tggacgagcc gttcaaccag gagcgtggtc
     8401 tttccggtcc ccgcgcccgc ggtcacgacc acgttggcgt cgaacgtgcg gatggcccgc
     8461 tcgcgttcga acgcatcgat cggaggagtc atcccggctc cttcggcggc cgcaccggcg
     8521 cgcggcgaat gcgatcgagg cccacggatc ggggatcccg ttccgcgcgc gcgcgcgacg
     8581 gcgcgtgccg acggcggcac gacgccgcca cctcgcacca cgcgcaatga gcgtcggggt
     8641 tcatcggaaa gactccctgt tcgatcccgt ccacgagcgc gcgcaccgtg cccaggattc
     8701 gagtccgcat gtccggatca ggcgcgtacg gctcgcgttc gatgccagcg tcgcgctcgt
     8761 tcaacccgac ctcgtagaca acggccgcgg attccccgac cgccttgcca aggcgggtga
     8821 gtgcgtaccg ttgggccaag gcttcgtaga tcggcggttg caacccgcgg ccgcgaagcg
     8881 cctcggccac gagttcgttc cggcggttcg ccccgcgcgc gtcgcggaac ttccagtcga
     8941 tgacccgcag gcctttgtcc ggtgcttgat cgaggcggtc cagacggccc tggatcggca
     9001 cgaccgggtc cgcgcccagt tccccgcgta acgccacttc ggtctcgatc accgtgtagc
     9061 cggaccgatc ccgctccccc gcgtcatccg ccgcgacctc ggccactacg cggccgatcc
     9121 gctcgacggc caggtcccag accaagggat acccgatgcc ggcgcgccgc gcgtaccgcg
     9181 cgcagacctc gcgccagatc gcattgatgt ccgacgccga atcccgcgcc gcccgcgcga
     9241 tcgcctcgtg agccaacagg ccccagtcac gcgccgtggg ccccgcgtcg acctcggcgt
     9301 cggacgacgg cgaaacatcc aacacccggg tcacgaaaaa ttgccacgga cacgccgcgt
     9361 aggtcgcaag ccccgtggcc gcgtaccccg cctctcgttg acgggcgagc cactcctccg
     9421 gcgtccccac gtccccgtcg aacgcggata atgtcgtcgc ccacgcctcg atcgcgtcgc
     9481 gacaggacgc ggcccggacg agccactcgc gagtccgtcc gtcggcaacc gccacggagc
     9541 gcacgccgac caactccgcc agcaccgagg cttcctgcgg cgtcaaccga tccacgcgag
     9601 cgaacggcgc cacgcggggt ttctcccgcg tccggcgcgg cacgtcgaca cgaggcgtat
     9661 tggacgcctc atcgcgccat ggcgccacgt accaggacgg cgccagcgcc cggcccgcgt
     9721 cgtcggcgcg ctgcgtcgag agcacgaccc gatctcggcc gccgccaccc gtgagcgcgg
     9781 caaacaacaa gcgctcctcc tcgtaccccg ccagcttctc cgggatcttg tagccgagcg
     9841 tcgtctcgat gaggcggcgc tcctgatccc gtaaaaacgc gtcctcgcgg atcacgcgcg
     9901 ggaacacgcc ttcattcagt cccaatagga acaaggcccg gcacgcggtg ccgcgcgcgg
     9961 atgcggcatc catcacctga acgccgcggg cgtgcgggtc ggtgagcggc aacgcggtcg
    10021 actcgatccg gcgacgcacc acgtcgagat acgcggcttt ggtcatggtc gccgtcacga
    10081 cgtccagccg agcgggcgca tcgaacgccg cgagcactgc ggacgccacg gcgtcaacgc
    10141 cgtccgggtc gtcatccgag gcatccgcct cgcgctcgat tcccaacagc gatgcgagta
    10201 acccgcgcca cgcgtcggcg tgggatgagc ccgaggctgt ttccggcaac gccccacacg
    10261 ctcgccgcag accgttcacc acttggagca agacggcggc ctgtgctccg cgcgaggagg
    10321 atcccgtctc cgggccgccg tcgggaccga caagagcggc caaccgcgac cacgcctcgt
    10381 atccgtgcct cacgccgacg gagcgcgcca actcatccca caacgcggga tcggcgtgga
    10441 cgtgcggcgc cagacgctcc acgcgacacc agggagatgc tacgaagtcg atcacgtcct
    10501 ctcgcgccgg agcaccctcc gcgatcctcg cgagtcgcaa cgcggcctgg gccagcgggt
    10561 gccgcgagag cggcctggtc gcggtggtga ccagggggag atgatgccgg gcgaactcgc
    10621 gcgcgatcgc gtccgcgtaa ggatcgaggc tcctcgacac cacgccgatg tcttcgaacg
    10681 ccagcccctc gtcttcgacc aaggcgagaa tggccttggc gcaggcgcgg acctcgtccg
    10741 tgacgccgga acaggacacg agcgtgaccg acggccggga cggcacgggg ccgtcgatga
    10801 atcgaaccgg tcgagtcgat actcccgtca ggtatcgggt gaggaatcga tccgcgaatg
    10861 cccacgacgg atcgccggac acgaacggga aatagaccgc ggcgtccgcc gcgcccgaca
    10921 cggcgcgcag cacgtcgtat tgggcctgag tcaggtcata gaatccgtag taggccacga
    10981 ggcgttgggt cttcagcatg cctgacgacg gcgcggccgc ggccgccaac acgtcgaggt
    11041 cggtataatc gacccacccg gccgtctcag ccgcggacaa gacctcgccg tacagaccgg
    11101 ccaaccccgc gagccgtgac gcggcttccg ccggaaacag ggattccgcc acggcctcgg
    11161 ccagcgcgcg cggcggcacc ttggcgtctt ttaagtcccg cagcgtctgc cacagcgccg
    11221 cgcccccgcc ccgcgtcgcc gcaaccccgg caaacgcgtg ccccgcgttc tcgcgatcaa
    11281 gcagcacccg catcagctct tcatacgcct gatcatccac gagcggcggc aggggccgac
    11341 ggatcgactc ttcgaccagg cggcgtgaca gttggtgaaa cgtcaggacc gacgcgctca
    11401 ggaacgcgcg cttccgctcc ccggcgagca aacggacgat ccgtcgcctg agcgagtcgg
    11461 acggaaccac gatgatgacg ggcgcgagcg gatcagccgc tttcacggcc atgacgtcgt
    11521 ccgcgagcgc ggtttcaaga ctggggtagg atccgcaggt gatggtcagc acggcgggag
    11581 agtcgcctca gtaggctgcc gaaaaacctt tcgggcgtcc ttcgacaggc tcaggacgag
    11641 gggaggaatg gccttggagg cttgaatcaa cgtcccgttt gtggtgcgcc cgtcgaacaa
    11701 cgttcgcccc gtgttcacca atccgttacg agacccgcat caattcgcgg atgggagtgg
    11761 gcctggattc tgatggtgat ctgggccgtc ggcccgcgat caggcgagcg acggttcggt
    11821 cggcgcgtcc gagacaggag agtcggcgga ctccacgatc tcccggacgt ccggcgatgc
    11881 gccggacgtc tcggtgtcgc tcgccggcaa ctcgccctcc gcgacctcct gatcgcccac
    11941 ggacgcgtcc gtggcgcgcg cgatctccgc gaagtctttg agcgtcggga gatcggcgag
    12001 atccttgagg cggaaggcgt gcaaaaattc cttggtcgtg ccgaacatca tgggtcggcc
    12061 gggaacgtct ttgcgtccga cgatcttgac gaggcgacgg tccatcagtg tcttgaccac
    12121 gcccgctacg tccacgccgc gaatcgactc gacttccggg cgggtcagcg gctgtttgta
    12181 cgcgatgatc gccaacgttt ccaacgcggg tttggagagc cgcggcgggg ctttggccgc
    12241 ggacaattta cggagccacg gcgccgcctc ctgaaccgtc acgagctggt agccgcccgc
    12301 cacttccgcc acggtcaacc cgtgatcgcc ccggcgatac tgctcccgca ggtcgtcgag
    12361 gcacgcgacc acccgctcgc gctccacgcc gtcgaggatc tcgcacaact tgttgagcgg
    12421 catgggttcg gccgcgacga acaacaacgc ttccacgatc ggtttgagtt cgcggtcatc
    12481 catcgagcgt atctccctgc gacgcgttca tctcgccgcc gtcttcagcc gggacggact
    12541 ccagccgcaa cacatcgatc tccgcgttcg gtcccgtctg acggagcccg agcacgtgca
    12601 ttttgaccaa ctccagcaat gccaggaacg tgacgatcac ggccgtgcgc gtggcgacgc
    12661 cctcaaacaa ctcgtggaac cgcacccgtt cgccctcggc caacgcgtcg aaccgctcga
    12721 tgatcgcggt catgcgatcg cgcacggtca acgcctccac cgtcacttcc atgatggtcg
    12781 ggtccggcac gcgatcgagc acgtcgcgca gcgcggtgag cagatcgaac accgacaact
    12841 cgcccagttc gtattct
//
LOCUS       DBNGTT010000043         3651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3329414, whole genome shotgun sequence.
ACCESSION   DBNGTT010000043 DBNGTT010000000
VERSION     DBNGTT010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3329414"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="AC16BC_01005"
     CDS             complement(<1..522)
                     /locus_tag="AC16BC_01005"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3240218.1"
                     /translation="MRELKLFTGNSNPALAQEICDYLGIPLGEATVGHFSDGEIQVRI
                     EENVRGMDVFLLQSASDPVNKHIMELLIMLDALKRASAYRITAVLPYYGYARQDRKDQ
                     PRVPITAKLVADLIATAGAHRVLTIDLHAGQIQGFFNIPVDHLYATPVLLEHFRQVEI
                     ADLVIVSPDAGGVE"
     gene            complement(545..619)
                     /locus_tag="AC16BC_01010"
     tRNA            complement(545..619)
                     /locus_tag="AC16BC_01010"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(585..587),aa:Gln,seq:ttg)
     gene            complement(641..1504)
                     /gene="ispE"
                     /locus_tag="AC16BC_01015"
     CDS             complement(641..1504)
                     /gene="ispE"
                     /locus_tag="AC16BC_01015"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3240219.1"
                     /translation="MIALRAPAKINLCLRVLSRRPDGYHDLESAFQMVGLYDDVTIRS
                     RRSGIALTVEGESLPEGPGNLAYDAAAALAREAGGPRGALIRLVKRIPLGAGLGGGSS
                     DAAAVLIGLNRLWTLRWSRARLAELGATLGSDVPFFFFGPTAWVTGRGERVASAPRWL
                     PSPAGVVPCWAVLANPGFAVSTKWAFEALGLTNTRQADKMPRSLKALPAAPWSIPNDL
                     ERVTAATYPVIEEMKTRLLKAGASVARMSGSGPTVFGVFATRAEAVNACEGLAPSWRR
                     WVVRLLRRAPW"
     gene            complement(1504..1764)
                     /locus_tag="AC16BC_01020"
     CDS             complement(1504..1764)
                     /locus_tag="AC16BC_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240220.1"
                     /translation="MIGDLLISIGVWLAYFSLIFFPLRWLYDRVLGPRPSDRHVDRDR
                     LTAWTARQKRLRRVFVSVGVGLAVLPLVVLVIVSLTKAAGEA"
     gene            1958..2575
                     /locus_tag="AC16BC_01025"
     CDS             1958..2575
                     /locus_tag="AC16BC_01025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3240221.1"
                     /translation="MANIYHAEHRQLQERFDTVRLAEKMESDLVQDFISDEDKAFLEG
                     MDMFFLATADQRGRVNCSYRGGDPGFVRVVDERTIAFPNYDGNGMYLSMGNIAQTTEV
                     GLLFIDFEQQSRMRLNGTAVIRHDDPLLGEYPEAQFIIRVTVRELFTNCRRYIHKMQR
                     VARSRFVPKAGCKTPVPGWKTGAWVKDALPANDPARDPTREALDR"
     gene            complement(2591..>3651)
                     /locus_tag="AC16BC_01030"
     CDS             complement(2591..>3651)
                     /locus_tag="AC16BC_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3240222.1"
                     /translation="KPFKIDEVKLIVKNALERKQLQEENSRLKQVVHDRASFEQIVGR
                     SDKLTKVLDLIRKVADTNSNVLIFGESGTGKELVARAIHYNSRRKDRPFVTVNCSALP
                     EPLLESELFGHMKGSFTGAISNKEGLFEIANEGTLFLDEIGDTPLGIQVKLLRVLQEK
                     EFRRVGGTRDLKSDVRIVAATNRDLEKAIAEGRFREDLYYRLDVIPIHLPPLRERPED
                     IPLLVDAFLQKFTREMGKSVMRIEPDAVALLCQQEWRGNVRELENVMERIVALASGGT
                     IETGQVRECVPRSSEDPVAMALPASGLDLEGVMATIEKDLLVKALHRANGVKKDAAGL
                     LGLDFRSFRYRLSKYGIK"
BASE COUNT          673 a         1260 c         1112 g          606 t
ORIGIN      
        1 ctccacgccc cccgcgtccg gggaaacgat caccagatcc gcgatctcca cctgccggaa
       61 gtgttccagc aacaccggcg tggcgtacag gtgatcaacg gggatattga agaacccctg
      121 gatctgcccg gcgtgcaggt cgatggtcaa cacccggtgc gcgcccgcgg tcgcgatcaa
      181 gtcggcgacg agtttggccg tgatcggcac ccgcggctgg tctttgcggt cctgccgggc
      241 atagccgtag tagggcaaca ccgcggtgat gcggtacgcg gacgcgcgct tgagcgcgtc
      301 cagcatgatc agcaactcca tgatgtgttt gttcacggga tcggacgcgg actggaggag
      361 gaagacgtcc atgccgcgca cgttttcctc gatgcgcacc tggatttccc cgtcgctgaa
      421 atgccccacc gtggcttccc cgagcgggat tcccaggtag tcgcagattt cctgcgcgag
      481 cgccgggttg gagttgccgg taaagagttt cagttccctc attgcctcac catcatccgg
      541 tcggtggttg gggcgccagg attcgaacct gggaatggga gatccaaagt ctcctgactt
      601 accgcttgtc gacgccccac catccgactc gacgcccgcg ttaccacggt gcgcgcctca
      661 acagccgcac cacccatcgc cgccacgagg gcgccaagcc ctcgcaagca ttgaccgctt
      721 ccgcgcgggt cgcaaagacc ccgaacacgg tggggccgct ccccgacatg cgcgccacgc
      781 tcgccccggc cttcaggaga cgggttttca tctcctcgat aaccgggtag gtggcggcgg
      841 tcacccgctc gagatcattc gggatcgacc atggcgccgc aggaagggcc ttcagcgaac
      901 ggggcatttt atcggcctgc ctggtgtttg tcaatccaag cgcctcaaac gcccacttgg
      961 tggacaccgc gaacccgggg ttcgccagca cggcccaaca gggcaccaca cccgcgggag
     1021 acggcagcca ccggggcgcg gaggccacgc gttcgccccg ccccgtcacc caggccgtcg
     1081 ggccgaagaa aaaaaacggc acgtcgctgc ccagggtggc gcccaactcg gccaggcggg
     1141 cgcgcgacca ccgcaacgtc cacaggcggt tgagcccgat gagcaccgcg gccgcatcgc
     1201 tgctgccgcc gccaagcccg gcgcccaggg gaatgcgctt gaccaggcgg atcaacgcgc
     1261 cgcgcggccc gccggcctcg cgcgccaagg ccgcggccgc gtcgtacgcc agattgccgg
     1321 gcccctcggg caacgactca ccctcgacgg tcagcgcgat cccggatcgt cgcgatcgga
     1381 tcgtgacgtc gtcgtagagg cccaccatct gaaacgcgga ttccaggtcg tggtatccgt
     1441 ccggccgccg actcagcacg cgcaggcaca gattgatctt ggccggcgcg cgaagcgcga
     1501 tcatcacgcc tctcccgccg ccttcgtcaa cgacacaatg accaggacca ccaggggcaa
     1561 gaccgccagc ccgacgccca ccgacacgaa cacccggcgc aaccgctttt gacgcgcggt
     1621 ccacgcggtc agtcggtcac ggtccacgtg ccgatccgac ggccgcgggc ccagcacccg
     1681 atcgtacaac cagcgaagcg ggaaaaagat gagcgaaaaa tacgccagcc acaccccgat
     1741 cgaaatcagc agatcaccga tcatgccaac gctcccacca ccgccctacc ataacgtagc
     1801 aagcgcgacg gacgcaatcg cgcttgagtc ggatgcgcgc cggctgctag agtggtggac
     1861 gatgcggcgc cgtttcgtcc tcgcacgggc gagacggcgt ggcgagctcg ttgacctcac
     1921 gcgccccaac acaaccaacg gttgtcggag gactgcgatg gcaaacatct atcatgccga
     1981 acatcggcaa ctgcaggaac ggttcgacac ggtcaggctc gccgagaaga tggagagcga
     2041 cctggtgcag gacttcatct cggacgagga caaggcgttt ctcgaaggca tggacatgtt
     2101 cttcctcgcc actgcggatc aacgcggacg cgtcaactgt tcgtaccggg gcggcgatcc
     2161 gggcttcgtg cgcgtggtgg acgagcgcac catcgcgttt cccaattacg acgggaacgg
     2221 catgtacctc tcgatgggca acatcgcgca gaccacggag gtgggcctgc tgttcatcga
     2281 ctttgagcag caatcgcgga tgcgcctgaa cggcacggcc gtgatccgcc acgacgaccc
     2341 gctgctcggg gagtaccccg aagcgcagtt catcatccgg gtgacggttc gcgagctgtt
     2401 caccaattgc cggcgctaca tccacaagat gcagcgcgtg gcgcgctcgc gttttgtgcc
     2461 caaggcgggg tgcaagacgc ccgtgccggg ctggaagacc ggggcctggg tgaaagacgc
     2521 gttgccggcg aacgacccgg cgcgggatcc gacgcgcgaa gcgttggatc gctgagccgc
     2581 acggcggcgg ctacttgatc ccgtatttgc tcagccggta acggaacgac cggaaatcca
     2641 aacccagcag acccgcggcg tctttcttga ccccgttcgc gcggtgcagc gccttgacca
     2701 ggagatcctt ctcgatcgtg gccatgaccc cctcgaggtc gagaccgctg gccgggagcg
     2761 ccatggccac ggggtcctcg gacgaccggg gcacgcactc gcgcacctgc ccggtttcga
     2821 tggtgccgcc ggatgccagc gccacgatgc gctccatcac gttttccaac tcgcggacat
     2881 tgccccgcca ttcctgctga cagagcaggg ccaccgcgtc cggctcgatc cgcatgacgg
     2941 atttgcccat ctcccgggtg aatttctgaa ggaacgcgtc caccagcagc gggatgtcct
     3001 ccggacgctc acgcagcggg ggcagatgaa tggggatgac gtccaaccgg taatacaggt
     3061 cttcccgaaa ccgaccctcc gcaatggcct tctccagatc ccgattggtg gcggccacga
     3121 tgcgcacgtc gctcttgaga tcgcgcgtac cgcccacgcg gcggaactcc ttttcctgca
     3181 acacacgcag gagcttcact tggatcccca gcggcgtatc accgatctca tccagaaaga
     3241 gcgtgccctc gttggcgatc tcgaacaggc cctccttgtt gctgatcgcc cccgtgaacg
     3301 atcccttcat gtgcccgaac agctcgctct ccaacaacgg ctccggcaac gcgctgcagt
     3361 tgacggtcac gaacgggcgg tccttgcgcc ggctgttgta gtgaatcgcc cgcgcaacca
     3421 gttccttgcc ggttccgctc tcgccaaaga tcagcacgtt gctgttggtg tcggccacct
     3481 tccggatgag atccaggacc ttggtcagct tatcgctgcg ccccacgatc tgttcgaacg
     3541 acgcccgatc gtgcacgacc tgcttgagcc gactgttctc ctcctggagc tgcttgcgct
     3601 ccaacgcgtt cttcacgatc aacttgactt cgtcgatctt gaacggtttg g
//
LOCUS       DBNGTT010000044         4507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1541528, whole genome shotgun sequence.
ACCESSION   DBNGTT010000044 DBNGTT010000000
VERSION     DBNGTT010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1541528"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1478)
                     /gene="urtA"
                     /locus_tag="AC16BC_01035"
     CDS             complement(138..1478)
                     /gene="urtA"
                     /locus_tag="AC16BC_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HIR3240223.1"
                     /translation="MKDRKAQSEAASGTDRREFLKRGALLGAGMVAGGIIGAPMINRF
                     ARAAEGPIKIGVLHSLSGTMAISEVSLRDVVMMAVEEINAKGGVMGRKIEPVVVDPAS
                     DWDLFAEKAKFLLLQEKVAVVFGCWTSVSRKSVLPVFEKNNGLLFYPVQYEGEECSKN
                     VFYTGATPNQQLIPAAEYLMSAEGGGYKKFYLLGTDYVFPRTANKILRAMLLAKGVPA
                     VNIAEEYTPFHHQDYQTIVQKIKAFAKAGDAAVLSTINGDSNVPFYKEFGNQGLRSED
                     CPIMAFSVAEDELRGMDTSALVGHLAAWNYYQSVDTPQNKEFVSKFKAYCKKAGLPGG
                     ESRVTDDPIEAAYFGVYLWKQAVEKAKSADVDKVLKAAPGQKFLAPGGEIKLDERNHH
                     THRPVLIGEILKDGQFKVVWRSKGLVKPDPWSEYTSPDKGCDWVDHQGTYQKKA"
     gene            complement(1581..2663)
                     /locus_tag="AC16BC_01040"
     CDS             complement(1581..2663)
                     /locus_tag="AC16BC_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR3240224.1"
                     /translation="MPAVAAVCWVALSGLAQAGGTIKLDDTKSISVGMGIRTSFNAVE
                     GAAPDGTSRSKDFALDSARLFLSGQILPKVSFEFNTEYTTVAGGAPGGDVQMMDAVLK
                     FAFNDYFNVWAGRFLPPSDRSNLSGPYYLNSWDFPFVQLYPNLAVGRDNGAAVWGQIN
                     KGQFKYQIGAFEGLGDSASGPNQSDSLLYAGRLVLNLWDPEPGYYNSSTYYGSMNVLA
                     LGLTTMTQSDAVGTAATPGDFTGWSLDVLAERKLGEAGVGTFEGAYYDYTYDDVSPDA
                     NGYFALLSYLLPQKFGAGQPQPIVRYQSLTTKANPDVDHTRLDIGVNYIIDGYNARLS
                     ALYFTDDPGGGASSFNGFKLGLQFQI"
     gene            complement(3374..3577)
                     /locus_tag="AC16BC_01045"
     CDS             complement(3374..3577)
                     /locus_tag="AC16BC_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3240225.1"
                     /translation="MYTCEGAIRVIYPYLDGELEVEETGRLQIHLQECPCCRAEFASE
                     HSFLDLVRSQMSVISTPTKACNF"
     gene            complement(3684..>4507)
                     /locus_tag="AC16BC_01050"
     CDS             complement(3684..>4507)
                     /locus_tag="AC16BC_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3240226.1"
                     /translation="RQGGTMRLVRDIMTTTVRWVQPGASARAAAALMSRHRIGSLLVE
                     DGDGSAAIVTETDLVRRVLAENKNPTSVTVDQIMTSPLITVDEHQSVKHAEEVMAEYE
                     IRHLGVTASAQVVGLISVRDLLREVQLLPIAVERMMTRMPVIVPTMETVRNSAALMAQ
                     SAVSGLLVCGQRRQPRGIHFSGFARSDIAGIATGTDLVRRVIDKDLDPNITPILEVMA
                     RPIYTVSASSDVMTAFNVMARAGVRHLGVVEGDEITGLLSVEDVIEPVWLHAAAG"
BASE COUNT          877 a         1445 c         1293 g          892 t
ORIGIN      
        1 tgagtcgcgc ctccgggtcg tcaccggcca agtcggcgac cagcccttcg ggtagcgcgg
       61 cccgagcagg caccgcaccc gtgagcgtag tcgctccgac gatggcccct atgaggagcc
      121 acgtgatcag cgacggccta tgccttcttc tggtacgtcc cttggtgatc gacccagtcg
      181 cagcccttgt ccgggctcgt gtactcgctc cagggatccg gcttgaccag acctttggat
      241 cgccacacga ccttgaactg gccgtccttc aggatctcgc cgatcagcac cggacggtgg
      301 gtatgatggt tccgttcgtc gagtttgatt tcgccgccgg gcgccaggaa cttctggccg
      361 ggcgcggcct tgagcacctt gtcgacatcc gcggatttgg ctttctccac cgcctgcttc
      421 cacaagtaca cgccgaaata cgcggcctcg atcggatcgt cggtcacgcg gctctccccg
      481 cccggcaatc ccgccttctt gcaatacgct ttgaacttgg acacgaattc cttgttctgc
      541 ggcgtatcga ccgactggta ataattccac gcggcgagat gccccacgag cgcggatgtg
      601 tccatccccc gcagttcgtc ctccgccacc gagaacgcca tgatcgggca gtcctcggac
      661 cgcaacccct ggttgccgaa ctccttataa aatggcacgt tgctgtcgcc gttgatggtg
      721 ctgagcacgg ccgcgtcgcc cgccttggcg aacgctttga tcttctgcac gatcgtctgg
      781 tagtcctgat ggtgaaacgg ggtgtactcc tcggcgatgt tgacggccgg cacgcccttg
      841 gccaggagca tggcgcgcag aatcttgttg gccgtgcgcg ggaacacgta gtcggttccg
      901 agcaggtaga acttcttgta ccctccgccc tccgcgctca tgaggtattc ggcggcgggg
      961 atgagctgct ggttcggggt ggccccggtg tagaacacgt tcttcgaaca ttcctcccct
     1021 tcgtattgga cgggatagaa cagcagtccg ttgttcttct caaagaccgg cagcaccgac
     1081 ttacgactca ccgacgtcca acacccgaag accaccgcga ccttctcctg gagcagcaga
     1141 aatttggctt tttccgcgaa caggtcccaa tccgacgcgg gatcgaccac cacgggttcg
     1201 attttgcggc ccatcacccc gcccttggcg ttgatctcct ccaccgccat catgaccacg
     1261 tcgcgcaggg agacctcgct gatcgccatg gtgccggaga gggaatgcag cacgccgatc
     1321 ttgatcggcc cctccgcggc gcgcgcgaag cggttgatca tgggcgcgcc gatgatcccg
     1381 ccggcgacca ttccggcacc gagcagggcg ccgcgtttca gaaattcacg acgatcggta
     1441 ccgcttgccg cttccgactg agccttacgg tccttcatgc ccatcgcgtt ctcctactcg
     1501 aagaaaggtg aatacccaca cccgttccgt acgcgtgaaa cggcgtcggg ctcgccatac
     1561 catcgacggc cgcgttgaaa tcagatttgg aactgtagtc cgagcttgaa cccgttgaag
     1621 ctcgacgcgc cgccgcccgg gtcgtcagta aagtacaggg cgctcagccg ggcgttgtac
     1681 ccgtcgatga tgtagttcac cccgatatcg aggcgcgtgt ggtcgacgtc gggattcgcc
     1741 ttggtcgtca acgactgata ccgtacgatc ggctgaggtt gacccgcgcc gaacttctgg
     1801 gggagaagat aactgagcag cgcgaagtac ccgtttgcat caggcgacac gtcgtcgtag
     1861 gtgtagtcgt agtacgcgcc ctcaaaggtc ccgactccgg cctcgccgag ctttctctcc
     1921 gcaaggacgt cgagactcca cccggtaaag tctcccggcg tcgcggccgt gcccaccgcg
     1981 tcgctttgcg tcatcgtggt cagccccaga gccaagacgt tcatcgaacc gtagtaggtg
     2041 ctgctgttgt agtagcctgg ctcgggatcc cacagattca agacgagtcg gccggcatac
     2101 aggagggaat cggattgatt gggcccgcta gcactgtccc ccagtccttc aaacgcgccg
     2161 atctggtatt tgaactgtcc cttgttgatt tgcccccaca ccgcggcgcc gttgtcacgt
     2221 cccactgcga ggttcggata caactggaca aagggaaagt cccacgagtt caggtagtag
     2281 ggaccgctca ggttcgagcg gtcgctcggc gggagaaacc ggccggccca gacgttgaag
     2341 taatcattga acgcaaactt gagaaccgcg tccatcattt gaacgtctcc gccgggggct
     2401 ccacccgcaa ctgtcgtgta ttcagtgttg aactcgaacg aaaccttcgg caggatctgt
     2461 ccgctgagaa acagccgcgc gctgtccagc gcgaaatcct tggaccggct ggtgccgtcg
     2521 ggcgccgcgc cctcgaccgc attaaaactg gtccgaatgc ccatgccgac gctgatggac
     2581 ttggtgtcgt caagcttgat cgtgccgccc gcctgcgcca gcccgctgag tgcgacccag
     2641 cagaccgcgg caacggccgg caccaagaca cctgtttgcc gtccgtatcg cgacacgagc
     2701 ccccccgcgg gaccacgggc ctcaggcgac aaccacccac gcgctaccgc tgatgcgcca
     2761 cgacatgtat gtctatgtcc tctcaatcgc tcaatccgcc acatctgttt cgatccccct
     2821 ttcagcttga ccatcctgga acgatgaacg cgtgaatgcc gggccggcac ataaaagact
     2881 tagagagcaa aactaaggcc agagcacgag aatggacttg gtgaccgatt atgatggcga
     2941 attccgcagc gcctgaaacc caaggggccg tcagagcgca acgtttcaac aagactgttg
     3001 agtccaacca cacaagaatg tctgaaccgt gacgacgcgt cagcgaatgg cgatttgccc
     3061 taatggtccg acgatcgaga aaggttgtgt ggccgctgga ggccccttcg gcaattgcac
     3121 gacatcatcg aatggcgtca ataagaagac ccccgatata aacgttacga tgagaaggtc
     3181 ttagggagaa gatggcgtaa cggcgaggcg tggggatgtt cggttatcgc tcgtactgac
     3241 gatcgaggct cttacggacg gatggagggc atctgcgctc gaaggtcgga cgcgacaaca
     3301 aaccctgacg gggaaaggag caaatcgacg ctcggccctt cagcggtttg gtgctctcct
     3361 ttcggagaag aagtcagaaa ttgcatgcct ttgtcggcgt ggaaatcact gacatctggg
     3421 atctaaccaa gtcgaggaat gagtgctccg acgcgaactc ggcacggcaa cacgggcatt
     3481 cctgtagatg gatctgtagc cgccctgttt cctcgacctc gagttcgccg tccaggtaag
     3541 gataaatcac cctgattgcg ccctcacagg tgtacatctg ccccacactg gacggcggtc
     3601 gaggttagct gcgaactgcc agcctgaacc atcacgcagt ctcgcgacag cgtatgaagc
     3661 gcgagggtgt tcattatcgg cgttcagccc gccgccgcgt gcaaccatac gggttcgatc
     3721 acgtcctcga cggacagcag tcccgttatt tcgtcgccct caaccacccc caggtgccga
     3781 accccggctc gcgccatcac gttgaatgcg gtcatgacgt cggaactggc cgacaccgta
     3841 tagatcggcc tcgccattac ctcgaggatc ggcgtgatgt ttggatcaag gtccttgtcg
     3901 atcacgcgac gcaccaagtc cgtccccgtc gcgatgccgg ctatgtcgga acgggcaaag
     3961 ccggaaaagt ggatcccacg cggctgtctg cgctgcccgc acaccaacag tccgctgacc
     4021 gcggactgcg ccatgagagc cgcggagttg cgcaccgtct ccatcgtggg gacgatgacg
     4081 ggcattcgcg tcatcatgcg ctcaaccgcg atcggcaaca gctgcacctc ccgcaagagg
     4141 tctcgcacag agatcaagcc caccacctgc gcgcttgccg tcacacccag gtggcggatt
     4201 tcatactccg ccatcacctc ttccgcgtgc ttcacggatt ggtgttcatc caccgtgatc
     4261 agcgggctgg tcatgatctg atccacagtc acggatgtgg ggttcttgtt ctcggccaac
     4321 acgcggcgaa ccagatccgt ttcagtcacg atggccgccg atccgtcccc atcctcgacc
     4381 aacaggcttc cgatccgatg ccgactcatc aaggctgcgg ccgctcgcgc cgaggccccg
     4441 ggctgaaccc accgcacggt ggtggtcatg atgtctctaa caagtcgcat tgtccctcct
     4501 tggcgag
//
LOCUS       DBNGTT010000045         3718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_648614, whole genome shotgun sequence.
ACCESSION   DBNGTT010000045 DBNGTT010000000
VERSION     DBNGTT010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_648614"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2391
                     /gene="glnD"
                     /locus_tag="AC16BC_01055"
     CDS             <1..2391
                     /gene="glnD"
                     /locus_tag="AC16BC_01055"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR3240227.1"
                     /translation="DPPARGEGLAVSKGVLHPLWDLGYTVGHSARRIRDCLDLSRRDS
                     TIRTALLEARWLAGDRPLFEQFVTAFASQLRKDAGAYVRQKLAEREAGYERYGSTVYL
                     LEPDLKKSKGGLRDLHYIGWLARACYGTAEWDDLVARGLLTETERRNLLDARDFLWVV
                     RNELHFHAGKAGDVLTFDEQVRLAAFFGFSDSPQLLGVERFMQQYYRLSTTLHHTSDR
                     FVRRLQRPSPWRRLITAVTAREIDGRAVVTRNAVLLPERRREALAADPRAVIALFAVS
                     QDSGRPVSESTLDAISRAVAAHHDEAYLQPSIQKQVFALFAAPSIGRTLDAMHRVGIL
                     ERVLPEFASARGLMQFNQYHKFTVDVHSIRAVQAAGALAGHDDRIAAVYRGIHRKDLL
                     HLALLLHDIGKGKGGDHSEIGEAIAVRVAERLGFDAHERGVLAFLVRHHLVMSQIAFR
                     RDLSDDKVLVRFARQVGQPELLQMFLVFTYADITAVGPGTWTAWKEDLLCVLYGKAME
                     TLAGSAVAGDGAARRAVEDAVRGLAGVRFPSLWLARQFEVMPNRYLVVTPPEQIARHL
                     DLVYRLPPDGVLVATRWEAEQQTTELTVATFDTIIPGLFSKLAGGLAALGFQILGAQV
                     FTRLDGVVLDTLWFQDPDYAGEPPAERIAELKRTLSAVVMGRQRVEDLFARGTRLPRM
                     RRLPHGAERTQVEVDNETSDAYTIVDVFATDAQGLLYVITRTLFELGLSIHSSKISTR
                     LDQIVDVFYVQEANGGKAVDPGRVDRIKHTLTDAVDTYVARAASGMTAAVAEPRSS"
     gene            2527..>3718
                     /locus_tag="AC16BC_01060"
     CDS             2527..>3718
                     /locus_tag="AC16BC_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR3240228.1"
                     /translation="MRMRMWGVVLAVGVAMGGQPQGCERAGKDRIAQPFDYLTREPGY
                     QPPVPDDVTLYQTNGPVTGGSVQALGEGVDGTIYAGTFGDGVYRKLPDQDRWIPADSG
                     PGDPFIMTFAATPDGAVFAGTIRGGLFRSDDGRRWVPSGDGLGNDQVAAIARDPRTEI
                     MYAGTGSGVYRSHDNGRHWTAANRGLEMVLARSLVLASDGTVFTGTGGNGLFVSRDHG
                     AGWTPINEGLSDDRGLRENFIRVLAVDRSGAIYAGSFGGGVFKTVNDGSTWTVMNEGL
                     TNFSIRGLAIGRDAMYVGTGDGVYRSDNGGRRWDSSSEGMPNTNVQSLLLARDGTLYA
                     GTSAGVAVRAPGGTWRAVDRGMLFPAVRALAVDPRRGLFAGTRVNGLYRSKDGGQAWG
                     VLNDG"
BASE COUNT          582 a         1160 c         1374 g          602 t
ORIGIN      
        1 gatccgcccg cccgcggtga agggctggcc gtgagcaagg gcgtgctgca cccgctgtgg
       61 gatctcgggt acaccgtggg ccacagcgcc cggcggatcc gagactgcct ggatctgagc
      121 cgtcgggaca gcacgattcg caccgcgttg ctggaagccc gatggttggc gggagaccgg
      181 ccgttgttcg agcagttcgt caccgcgttc gcgtcgcagc ttcgcaagga cgccggggct
      241 tacgtcaggc agaaactggc cgaacgggag gccggctacg aacggtacgg ttcgaccgtc
      301 tacctgttgg agccggacct caagaagagc aagggcgggc tccgggacct gcattacatc
      361 ggctggctgg cgcgggcgtg ttatggaacc gccgaatggg acgatctggt cgcgcgcggc
      421 ctgctgaccg aaaccgaacg ccgcaacctg ctcgatgcgc gcgattttct ctgggtcgtc
      481 cgcaacgaac tgcactttca cgccggcaaa gcgggcgacg tgctgacgtt cgacgaacaa
      541 gtccggctgg cggccttctt cgggttttcg gacagcccgc aactgctcgg ggtcgagcgg
      601 ttcatgcagc agtattaccg gctgtccacc acgctccacc acacgagcga ccgattcgtt
      661 cgccggctcc agcgcccgag tccgtggcgc cggttgatca ccgcggtgac ggcgcgggaa
      721 atcgacggcc gcgcggtggt gacgcggaac gcggtgctgc tgcccgagcg ccggcgcgag
      781 gcgttggccg ccgacccccg cgcggtgatc gcgctgttcg cggtgtccca ggacagcggg
      841 cgaccggtta gtgaatcgac gttggacgcg atttcgcggg ccgtcgccgc tcaccacgac
      901 gaggcctacc ttcagccgtc gatccagaaa caggtgttcg cgctgttcgc cgcgccgagc
      961 atcgggcgaa ccctggacgc gatgcaccgc gtgggcatcc tggagcgggt gctccccgag
     1021 ttcgcgtcgg cgcgggggtt gatgcagttc aaccagtatc acaagttcac cgtggacgtg
     1081 cacagcatcc gggcggtaca ggccgccggc gcgctcgccg ggcacgacga tcgcatcgcg
     1141 gccgtgtatc gcggcatcca ccgcaaggat ctcctgcacc tggcgctgtt gctgcacgac
     1201 atcggcaaag gcaagggcgg agaccacagc gagatcgggg aggccattgc cgtccgcgtc
     1261 gcggagcgac tggggttcga cgcgcacgag cgcggggtcc tcgcgttcct ggtccgccat
     1321 catctcgtga tgtcccagat cgcgttccgc cgcgatctga gcgatgacaa ggtgttggtg
     1381 cgcttcgccc ggcaggtggg tcagccggaa ttgttgcaga tgttcctcgt cttcacctac
     1441 gccgatatca cggcggtcgg gccgggtacg tggacggcgt ggaaggaaga cttgctgtgc
     1501 gtcctctacg gcaaagccat ggagacgttg gcgggcagcg cggtagccgg cgacggcgcc
     1561 gcgcgccggg ccgtcgaaga cgcggtccgc ggcctggcgg gcgtccggtt cccgtcgttg
     1621 tggctggctc gtcagttcga agtcatgccg aatcggtatc tggtcgtgac gccgccggag
     1681 cagatcgcgc gccacttgga tctggtctat cggctgcctc cggacggggt gctggttgcg
     1741 acccgttggg aggccgagca gcagaccacc gagctgaccg tcgcgacatt cgatacgatc
     1801 atcccgggcc tgttctcgaa actcgccggc gggctggccg cgctggggtt ccagattctc
     1861 ggcgcccagg tgttcacccg gctcgacggc gtcgtgctcg atacgctgtg gtttcaggac
     1921 ccggattacg ccggagagcc tccggccgag cgcattgccg aactcaagcg gacgctgagc
     1981 gccgtggtca tggggcggca gcgggtggag gatttgttcg cccggggcac gcgcctgccg
     2041 cggatgcgcc ggctcccgca cggggccgag cggacgcagg tggaggtcga caacgaaacg
     2101 tcggatgcct acaccatcgt cgacgtgttc gcgaccgacg cgcaggggtt gttgtacgtc
     2161 atcacgcgca cgctgttcga actggggttg tcgatccatt cgtccaagat ctccacgcgc
     2221 ctggatcaga tcgtggacgt gttttacgtg caggaagcga acggcgggaa agcggtggat
     2281 cccggccggg tggaccggat caaacacacg ctgaccgacg cggtcgatac ctacgtggcg
     2341 cgcgccgcgt ccgggatgac ggccgccgtg gccgagcccc gctcgtcgtg atcgcatggt
     2401 gagcgcggca accgttgcgt tccgggtctc gtcggtgatg gcgtgtgatc gccgcgccgc
     2461 ggaccgcgag cctcgccgtt gtatcgcctc ggaccgcggt gaccggtgtg aaggggagga
     2521 gcgcgcgtga gaatgcgaat gtggggggtg gtgctcgcgg tcggcgtggc catgggcgga
     2581 cagccgcagg gatgtgaacg ggccgggaag gaccggatcg cgcaaccctt cgactacctg
     2641 acccgcgagc ctgggtacca gcccccggtt ccggacgacg tgacgctgta tcagaccaac
     2701 gggccggtca cggggggcag cgtgcaggcg ttgggtgaag gcgtggacgg gaccatatac
     2761 gccgggacgt tcggcgacgg cgtgtatcgg aaactcccgg atcaggatcg ctggattccg
     2821 gccgactccg ggccgggcga ccccttcatc atgacgttcg cggcgacgcc ggacggggcg
     2881 gtgttcgcgg ggacgatccg gggcggcctg tttcgcagcg acgacggccg ccggtgggtc
     2941 ccgtcagggg acggcctcgg caacgatcaa gtggcggcga tcgcgcgcga tccccggacc
     3001 gagatcatgt acgccgggac cggatccgga gtgtatcgaa gccacgacaa cggacggcac
     3061 tggacggcgg ccaaccgcgg cttggaaatg gtgctcgcgc gatcgctggt cctggcatcc
     3121 gacggaaccg tgttcaccgg gacgggcggc aacggcctgt ttgtaagccg ggatcacggg
     3181 gcgggatgga ccccgatcaa cgaagggctg tcggacgatc gcgggttgcg ggagaacttc
     3241 atccgggtgc tggcggtgga tcggtcgggc gccatctatg ccgggagctt cggcggcggc
     3301 gtgttcaaga ccgtgaacga cggcagcacg tggaccgtga tgaacgaggg gttgaccaac
     3361 ttctcgatcc gcgggctggc gatcggccga gacgcgatgt atgtcggaac cggggacgga
     3421 gtgtaccgca gcgacaacgg cggtcggcgc tgggactcga gcagcgaggg catgccgaac
     3481 accaacgtcc agagtctcct gctcgcccgc gacggcacgc tctacgccgg gacgtcggcc
     3541 ggcgtcgcgg tcagagcgcc gggcgggacc tggcgcgcgg tcgaccgggg gatgctgttt
     3601 cccgcggttc gagccctggc ggtggacccg cgtcgcgggc tcttcgccgg cacacgggtc
     3661 aacggcctct accggagcaa ggacggcgga caggcgtggg gggtgttaaa cgacggct
//
LOCUS       DBNGTT010000046         3812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_648666, whole genome shotgun sequence.
ACCESSION   DBNGTT010000046 DBNGTT010000000
VERSION     DBNGTT010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_648666"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="AC16BC_01065"
     CDS             complement(<1..1404)
                     /locus_tag="AC16BC_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3240229.1"
                     /translation="MRSPLRIPLIVACGLFIENMDSTVIATALPAIAGDLRVDPIALK
                     LALTSYLLGLAVFIPISGWVADRVGARTTFASAVGVFMLGSLACAASSSLEAFVASRF
                     VQGIGGAMMVPVGRLVLMRSIPKSELIAALNYLAMPALIGPVIGPPIGGFITTYFHWR
                     WIFLINIPICVLGIWLALRFIPNFREPDQPRFDLLGFVLSGIGLSAAMLGLATLWEHM
                     LSPRASAVCVAIGAVALTAYGRHARRTEHPLLDLRLFRLPTFRAGVIGGAVFRAGQGA
                     VPFLLPMMLQLAFRLTPLQSGFLTFAAAVGALFMKTLTTVILGRWGFRTVLSVNAVVA
                     SVMLSVCGLFTIETPHAVILTVVLIGGCLRSLQFTSLNAICFAEVSQREMSQATSLMS
                     VGQQLSMSLGVMVGAYALQGAHAFRGGPDLVSQDFRIAFFVVSAVAAVSVISFLKLAP
                     TAGAELAGPRVLTETEQR"
     gene            1714..3396
                     /locus_tag="AC16BC_01070"
     CDS             1714..3396
                     /locus_tag="AC16BC_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HIR3240230.1"
                     /translation="MRTKDAIRELLTRLDGEAADAIEDHELECKPWDDDTKTLHRMLR
                     ETVVCLANARGGTIVLGVRDRVRTRREAIQGVGRYDLQALRRSIYDGTDPHILVEIEE
                     LVEPEGTLLLVRVPKGMPPHTTSDGVARIRIGKECKPLTGRTFAQLLASGGQRDPTAE
                     VVPSVGEADLDPAEVARLRTVIQRESQNADLARLESQQLLAALGLVSDAGVTLAAVLL
                     VGRAGTIARHVPQNEVTFLRYRSSTRYDQRKDLRGPLLSILEELERLVSVNNRVRTIQ
                     EEGFGQLEFPDLSWEVAREAVLNAVTHRDYFLRQSTQVALHRDRLKVTSPGGFVGGVT
                     AENVLRHAPVHRNELLARACQAAGLVNRVGLGVDRIYFELLRLGKDAPRYEADESHVD
                     LTIPLQTHEAFALFVAREERQGRPLELDDLLVLRALVGRSMVDRWSAARILQLSEDEA
                     ASRLISLRERGYLVVRGRGRGASYDLRRDLASRFRCRAAVDAELPLDEEAVRLRILAL
                     LREHGRLTNAEIRRFSGFTRAQVYRIVKGLEAEGQARFAGRGRAGHVLPGKG"
     gene            3546..>3812
                     /locus_tag="AC16BC_01075"
     CDS             3546..>3812
                     /locus_tag="AC16BC_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240231.1"
                     /translation="MLSPEIQALLDGLHPLEARVFRAFGEQSSASEAALREGSGMAAS
                     QLSMALGWLLTKGAVRVESERVTRRAALADAGKRYATDLIPEIRI"
BASE COUNT          781 a         1101 c         1209 g          721 t
ORIGIN      
        1 gcgctgttcg gtctcggtca acacgcgtgg tccggcgagc tcggcgccag ccgtcggcgc
       61 cagcttcaag aacgagatga ccgacactgc cgcgaccgcg ctgaccacga agaacgcaat
      121 ccgaaagtcc tgactgacga ggtccggccc tccgcgaaac gcgtgggccc cctgaagcgc
      181 gtaggcgccg accatcacgc ccaaactcat cgacaattgc tgccccacgc tcatcaggct
      241 cgtggcctga ctcatttcgc gttgcgacac ctcggcgaag cagatcgcat ttaaactcgt
      301 gaactgcagc gagcgcaggc agccgccgat cagcaccacg gtcaggatca ctgcgtgcgg
      361 agtttcgatg gtgaacaatc cgcagaccga caacatcacg gacgccacca ccgcgttcac
      421 actcagcacg gtgcgaaagc cccaacggcc caggatcacc gtggtcaatg ttttcatgaa
      481 caaggcgcca accgccgcgg cgaacgtcag aaagcccgac tggagcgggg tcagcctgaa
      541 cgctagttgc agcatcatgg gcagcagaaa cggcaccgcg ccttgtcccg cccgaaacac
      601 cgccccgccg atcacgccgg cgcggaacgt gggaagccgg aacaaacgca gatcgagcaa
      661 cggatgctcg gtccgacggg cgtgccgccc atacgcggtg agagcgacgg ccccgatcgc
      721 gacgcagacg gccgatgcgc ggggcgagag catgtgctcc cagagcgtgg cgagccccag
      781 catggccgcg gagagcccga tgcccgacag cacgaatccg aggaggtcga accggggttg
      841 atcgggttca cggaaattcg gaatgaaccg caacgcgagc cagatgccca gcacgcagat
      901 cggaatgttg atcaggaaga tccaccgcca atggaaatac gtggtgatga acccgccgat
      961 gggcggtccg atcacgggcc cgatcaaggc gggcatggcc agatagttca acgcggcgat
     1021 gagttcgctc ttgggaatcg accgcatcaa caccagccgt ccgaccggca ccatcatggc
     1081 cccgccgatg ccttgcacga atcgcgacgc cacgaacgct tcgagcgaac tgctggccgc
     1141 gcacgcgagc gagccgagca tgaatacgcc gaccgccgac gcgaacgtgg tccgcgcgcc
     1201 cacgcggtcc gcgacccacc cgctgatcgg gatgaacacc gccaggccga gcagatagga
     1261 cgtgagcgcg agcttgagcg cgatcggatc gacccggaga tcgcccgcga tcgcgggcag
     1321 cgcggtcgcg atgactgtgc tgtccatgtt ttcgatgaac aggccgcagg cgacgatcag
     1381 cgggatacgg agggggctgc gcatggaaga gatttagggg ggagtctgcg aaacgttgga
     1441 agccgttatg cgcatccctt aatattgcca agagccagtg cctttttata tcgcattctc
     1501 cgaaaaacgg cttcttttat ggtaaatagc cgatctcttg cgaaagagat tcgttgaatc
     1561 aagtggcgaa gaatgggacg ccgggggagt atcttaaccg ggaaaagcat tctgttccca
     1621 atgcttttcc gcgaataatt aattgactaa taatcattta tagtaatgtt ctattattga
     1681 catcggtata tccttatcat tatgatggct ttcatgcgga cgaaggacgc aattcgagag
     1741 cttctaacgc gtctagacgg cgaagccgcc gatgcgatcg aggatcacga actcgagtgc
     1801 aaaccgtggg acgatgacac gaagacccta caccggatgc ttcgggaaac cgtggtgtgt
     1861 ctggcgaacg cccgtggcgg gacgatagtc ttgggggtac gggatcgtgt ccgcacacgg
     1921 agagaggcga ttcagggcgt tggccgatac gacctccagg ctcttcgtcg gtcaatctat
     1981 gacggcaccg acccgcacat actggtcgag atcgaggaac tcgtcgagcc tgaagggaca
     2041 ctcctcctcg ttcgcgtccc caagggaatg ccaccgcata ccacgagtga tggtgtcgcc
     2101 cgaatccgaa ttgggaagga atgcaaaccg cttaccgggc ggacgtttgc ccaactgttg
     2161 gccagcggtg ggcagcgcga cccgacagca gaggttgtgc catcggttgg tgaagctgat
     2221 cttgatcccg cggaagtcgc gcgactccgg acggtcatcc agcgcgagtc ccagaacgcc
     2281 gacctcgcgc gtcttgaatc ccagcaatta ctcgcggcgc ttgggctggt ctcagatgcc
     2341 ggggtgacac ttgccgcggt cctcctcgtg ggccgggcag gaacgatcgc caggcacgta
     2401 ccccagaatg aagtgacgtt cctccgatac cgaagcagca cccgctacga tcagcgtaag
     2461 gatttacggg gaccactgtt gtccattttg gaagagctcg agcggttggt gtcggtgaat
     2521 aatcgggtgc ggacgatcca agaagaagga ttcggtcagt tagagttccc ggacttgtcc
     2581 tgggaggttg cgcgcgaggc ggtcctcaac gccgtgactc atcgcgatta tttcttgcgt
     2641 cagagtacgc aggttgccct ccaccgcgac agattgaagg tgacgagccc tggcggattt
     2701 gtgggcgggg tgactgccga gaatgtcttg cggcacgctc ctgttcatcg caatgaactc
     2761 cttgctcgtg cgtgtcaggc cgcgggtttg gtaaaccggg tggggctcgg ggtcgatcgg
     2821 atttactttg aacttcttcg gctcggtaag gacgcacccc gctacgaagc ggatgaaagt
     2881 catgtcgatc taacgattcc gctacagacg catgaggcat ttgccctctt cgtcgctcgc
     2941 gaggaacgcc aaggcaggcc attggagctc gatgaccttt tggttctgcg tgcgctcgtc
     3001 ggtcgttcga tggtcgatag gtggtctgct gcccgcatcc tccaactatc ggaagatgaa
     3061 gctgccagta gactgatcag tctgcgcgag cgcggctatt tagtggtccg tggtcgcgga
     3121 cgaggagcca gctacgatct ccggcgcgat ctcgcatcac gtttccgttg ccgtgccgct
     3181 gttgatgccg aattgccgtt agacgaggaa gcggtgcggc tacgtattct tgcgctgctg
     3241 agggagcacg gccgattgac gaacgcggaa atcaggcgct tttcgggttt caccagggct
     3301 caggtgtacc gaatcgttaa agggttggag gcagaggggc aggcacgatt tgcgggacgt
     3361 ggtcgcgctg ggcacgtcct acctggaaaa ggatgaggag tcccccatta tgtgataatt
     3421 gattagaggt tgaagttgct gcgtatttgt ttcaggttct gcggcttggt cgatcccgta
     3481 ctgtggatag ctttctcctg tcgtttcgtt gacttcaccc aggccctttg ctagcatcgc
     3541 gccctatgct ttccccggaa attcaggcgc ttctggacgg gctgcacccg ctcgaagcgc
     3601 gggtgtttcg cgcgttcggc gagcaatcgt ccgcgtcgga ggccgcgttg cgcgagggca
     3661 gcggaatggc ggcgtcgcag ttgtccatgg cgttgggctg gctgctgacc aagggcgcgg
     3721 tgcgggtgga gagcgagcgc gtgacgcggc gcgcggcatt ggccgacgcg gggaagcgct
     3781 acgcgaccga cctcatccct gagatccgca tc
//
LOCUS       DBNGTT010000047         2313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1414975, whole genome shotgun sequence.
ACCESSION   DBNGTT010000047 DBNGTT010000000
VERSION     DBNGTT010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1414975"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC16BC_01080"
     CDS             <1..469
                     /locus_tag="AC16BC_01080"
                     /inference="COORDINATES: protein motif:HMM:NF044669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240232.1"
                     /translation="ADRLWTLVLQRVADEKPNLESYLRSGRPVAAETGELVVEYGAAD
                     VWAADLASREDNQAFVARVLRDVAGRDLTFRIRRNADSPAGRAAPSATAERPVAPAGA
                     ESAPAGREGASRRAVVAEVLAHPAVKDAVEIFDGEVMDVRELNEPGEQRDAEG"
     gene            485..808
                     /locus_tag="AC16BC_01085"
     CDS             485..808
                     /locus_tag="AC16BC_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR3240233.1"
                     /translation="MLGNKRMMGDLMRQAQALQERMAKLQEEAGNRTVQGSAGGGMVT
                     VTANGRQEVLSVKLDPEIVKSQDQEMLQDLIVAGVNDALKKAQAMMAEEMKSVTGGLN
                     IPGLF"
     gene            819..1412
                     /gene="recR"
                     /locus_tag="AC16BC_01090"
     CDS             819..1412
                     /gene="recR"
                     /locus_tag="AC16BC_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3240234.1"
                     /translation="MVNGVFQRVVDSFKRLPGIGQKTAQRLGFYLLKMPESEAAAIAQ
                     AILDLKAQIQFCPVCQNMTEAPLCEICRDPGRDRTRILVVEEPSTVYAIERTGEYQGL
                     YHVLLGAISPLDGVKPEDIKAKELVDRLGSGDVHEVIMATDPNIEGEATALYLTRLIK
                     PLGIKVTRLAYGIPVGIDLEFADDVTLIKSLEGRRDF"
     gene            1754..>2313
                     /locus_tag="AC16BC_01095"
     CDS             1754..>2313
                     /locus_tag="AC16BC_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HIR3240235.1"
                     /translation="MESPSLIGTANKKGQVYTDPRKLFFEAQRTPSFLTGSEVIKEAI
                     RRASIDVMVAYPITPQSEAAALIGELFAEGYVGDYMRGESEFAVMSQCAGAAFGGARV
                     FTTTAGPGTLRAYENFPMWAGARLPIQVIVTCRGVNSPLSIQPDTLEIGMILDTGMMI
                     WHAESAQDFFDWILMGYIVAEQPDVHL"
BASE COUNT          432 a          625 c          826 g          430 t
ORIGIN      
        1 tgcggaccgg ctctggaccc tggtgctgca gcgggtcgcg gacgaaaaac ccaacctgga
       61 atcgtatctg cgcagtgggc ggccggtcgc cgcggagacc ggcgagttgg tggtggagta
      121 cggggcggcc gacgtgtggg ccgctgattt ggcgtcgcgc gaggacaacc aggcgtttgt
      181 ggcgcgggtg ctgcgcgacg tggccggccg cgacctgacc ttccggattc ggcgtaacgc
      241 ggattcaccg gcgggccgcg cggcgccgtc tgcgacggcc gaacgtccgg ttgcgccggc
      301 gggcgccgag agcgcgcccg ccggacgcga aggggcgtcg cgccgagcgg tggtggccga
      361 ggtgttggcg cacccggcgg tgaaggacgc ggtggagatt ttcgacggcg aggtgatgga
      421 cgttcgcgag ttgaacgagc ctggggaaca acgggacgca gagggatgac gcggaggcaa
      481 ggccatgctg ggaaataagc ggatgatggg tgatctcatg cggcaggcgc aggcgcttca
      541 agagcgcatg gccaagctcc aggaagaagc gggcaacagg acggttcaag gctcggcggg
      601 cggcggcatg gtcacggtga cggcgaacgg tcggcaggaa gtcctctccg tcaaactcga
      661 tcccgagatc gtgaaatcgc aggaccagga gatgttgcag gacttgatcg tggccggcgt
      721 caacgacgcg ttgaaaaagg cgcaggccat gatggccgag gaaatgaaaa gcgtgaccgg
      781 cgggctgaac attcccgggc tgttctgagc gaacgggtat ggtcaacggc gtgtttcaac
      841 gggtggtcga ttcgttcaag cgcctgcccg gtattggcca aaaaaccgcg cagcggctgg
      901 ggttttacct cttgaagatg ccggagagcg aggccgccgc gatcgcgcag gcgatcctgg
      961 acctgaaagc gcagattcaa ttctgtccgg tgtgtcagaa catgaccgag gcgccgttgt
     1021 gcgagatctg ccgggatccg gggcgagacc ggacccgtat tctcgtcgtg gaagaaccga
     1081 gcacggtgta tgcgatcgag cggaccggcg agtatcaagg gctttaccac gtgctgctgg
     1141 gcgcgatttc tccgctggac ggcgtgaagc ccgaagatat caaggccaag gaactggtgg
     1201 accgtcttgg cagcggcgac gtccatgagg taatcatggc gacggacccc aacatcgagg
     1261 gggaagccac ggcattgtat ctgacccggc tgatcaaacc cttgggcatc aaggtaaccc
     1321 ggctggccta tggcattccg gtcggcattg atctggaatt tgccgacgac gtgacgttga
     1381 tcaagtcgtt ggagggtcgg agagatttct agatagtccg gatttggaat acgggtcggc
     1441 gtgggccgag agccctagct cctcgggagg tcctggggct ttttctccgt ggcggggggc
     1501 ttttgccgcg ttgacttgcc gcgcgcgtcc gcggtaaggt ggagcctctc agtcgggttt
     1561 ggggatggac cgttcgaact cctcgaattc aattgacaat ctctgaagtg ctgcactatg
     1621 attgcactaa cttttggtgc gagtgtttcc gcggagtcga gtacgccagc ctgtcgtcag
     1681 ctgacccaga tcagtcgggc gactgagatc aatacggact agtcgtgtgg tccatcactg
     1741 aggggagggg gccatggaat caccatcgtt gatcggaacc gccaacaaga aggggcaggt
     1801 ctataccgat ccccgcaagc tcttttttga agcgcagcgc acgccgtcgt ttctcaccgg
     1861 tagcgaggtg atcaaggaag cgattcgccg cgcgagcatc gacgtgatgg tggcctatcc
     1921 catcacgccc cagagcgagg cggccgcgtt gatcggcgag ctcttcgccg aagggtacgt
     1981 gggcgactac atgcgcggcg agagcgagtt cgccgtgatg tcgcagtgcg cgggcgcggc
     2041 gttcggcggc gcgcgcgtgt tcaccacgac cgccggcccc gggaccttgc gcgcatatga
     2101 gaatttcccg atgtgggcgg gcgcgcgtct ccccattcaa gtgatcgtga cctgccgcgg
     2161 cgtcaactct ccattgagca ttcagcccga tacgttggaa atcggcatga tcctcgatac
     2221 cgggatgatg atctggcacg ccgagtcggc tcaggatttc tttgattgga ttctgatggg
     2281 gtatatcgtg gcggagcagc cggacgtgca tct
//
LOCUS       DBNGTT010000048         6199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_393265, whole genome shotgun sequence.
ACCESSION   DBNGTT010000048 DBNGTT010000000
VERSION     DBNGTT010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_393265"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..524
                     /locus_tag="AC16BC_01100"
     CDS             318..524
                     /locus_tag="AC16BC_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013185986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR3240236.1"
                     /translation="MGDSVVKVIELLSESDKSWEDAAQQAVSKAGKSLHGIKSIYIKN
                     FEGKVEGNKIVKYRINAQVSFQLD"
     gene            complement(521..1456)
                     /locus_tag="AC16BC_01105"
     CDS             complement(521..1456)
                     /locus_tag="AC16BC_01105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3240237.1"
                     /translation="MNALPEPLRDPSETNETTIGMADVSPLRRLEREERSLACLVVIA
                     GLSIGEKIALSKARTLIGRQADADIRFDDPLVSRHHAEIVAQPDGTAILRDLGSRNGT
                     FCNDKRITERALHEGDVIWVGRAVLKYLGPNSAESVYVSVMGDRERVDALTGLINRRT
                     FLEYLKRLMLRCLSLQEPLSVILISVDPFNLPNDSWSQPAGDYVVKELAAMFKKAMRP
                     TDLVARYGGEELALILPHTTAVEAVAAAERIRTSVATRHLMFAGQRLSVTISLGVADL
                     STGMEGPESLMESANRALADAKQQGRNRTACSPEA"
     gene            1546..2646
                     /locus_tag="AC16BC_01110"
     CDS             1546..2646
                     /locus_tag="AC16BC_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HIR3240238.1"
                     /translation="MPPLSSDFLIPFTEDAAQPLDWMDEIDAKPEPEWASWPDERLLD
                     ARLCDLNLSIDGSPLVQRIDQLATELADRRIVFRPHVWISDEWFTPDGIPGIAVPFYL
                     VHPRLVKLELQQMLEVEGGTHEWCMRILRHEAGHAIENAYRLRLRTRRKQLFGRSNVP
                     YPNFYTPRPYSKRFVLHLDMWYAQSHPDEDFAETFAVWLNPQSDWGAQYAGWPALKKL
                     EYMDALMRDLADVPPPVTRTDQVDPLPSLTKTLREHYQARRSHYGLEHPHFYDQDLQR
                     LFVEAAAAPGATSAAQFIARMRKEARRRVADRTGAYQYTIDRLLESMIARCRELDLRL
                     ANAPEQAKLDFMLLLTVQTMNFLHSGRHRVAL"
     gene            2906..3931
                     /locus_tag="AC16BC_01115"
     CDS             2906..3931
                     /locus_tag="AC16BC_01115"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3240239.1"
                     /translation="MKKLRIAALMHEDLIPPDDLGGIDLDAAEWKTEYDVVKALRCMK
                     HDVLPIGVRSDLGLLRKTIADFKPDIAFNLLEEFNDVPLWDQNVVSFLELMGVPYTGC
                     NPRGLMLARDKGLSKKLLSYHRIPFPDFMVVPIGRKPRRPKRLDFPLIVKSVCEEASL
                     GISQASIVTDDDKLRERVAFMHESVKTPAIAERYIAGRELYVGVLGNEKLQVLPIWEL
                     DLSRMPEDAPKIATRRVKWSLPYQKKYNITSGVAKDLPNGLSDRIHHLAKRVYRVLGL
                     SGYARLDMRLDPQGHVYIIEANPNPQIGHGEDFAESAKVVGLSYKALLQRIVTLGLAW
                     RPDRLAA"
     gene            complement(4022..4957)
                     /locus_tag="AC16BC_01120"
     CDS             complement(4022..4957)
                     /locus_tag="AC16BC_01120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3240240.1"
                     /translation="MSVNPKPIPFARDTDETTVGMADASPKRRLNRQERSLACLVVVA
                     GLSIGEKIALSKDRMVIGRQADSDICLDDALVSRHHAEIITRPDGTSVLRDLNSRNGT
                     LCNDQPITEHILRDGDVMWIGQSVIKYLAPDSAENLYLSAMADRARLDGLTALVNRRT
                     FQEYLERLVSRCRSLREPMSVVLFDVDHFKRVNDTWGHLAGDSVLKELASLLKRAVRP
                     TDLLARYGGEEFGLLVPYTTVIEALSVAERMRTIVAGHRFVFDGRLIPVTISLGVADL
                     VTQIESADALLQAADKALYAAKQQGRNRTVCSAEI"
     gene            5200..>6199
                     /locus_tag="AC16BC_01125"
     CDS             5200..>6199
                     /locus_tag="AC16BC_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3240241.1"
                     /translation="MIDVIELVIGLLIGVAVLAVVANRLQVPTAIVLVIGGAMFGLIP
                     GLPSVHLDPELVFLFFLPPLLYPAALYTSWRDFRANIRPISLLAVGLVLFTTLVIAWL
                     AHRFMGLPLAAGFVLGAIVSPPDAVAATAITQRLRVPRRIITILEGESLVNDATALVA
                     YRFAVAAVVTGMFSWTDAGWRFLVAGVGGIAFGWGVGRVAAWVQQRLEDPAIKVTVSL
                     LTPFAAYVPADRLGVSGILAVVTAGVYLGWRSPEITNASMRLQVRPVWSTITFLLNGF
                     VFILIGLQLSEILDSLSGYTAGQLAWYATLISLATILVRMIWVFPAAYLPRWLFKSI"
BASE COUNT         1100 a         2028 c         1912 g         1159 t
ORIGIN      
        1 cgagaaatac gcgatgcgtg gttcgcgggt cacgttttcc aacatacgga tcctcgatga
       61 gggttcccgt tgcgagcggc cgcgggtcca tccccgcgac gggcatgctt cgacacgaag
      121 ctatcacaat cggcccggct gagtcatggt cgtggtcggg ccctactcct tctcgggtga
      181 gaccgactcc cccgttatat ccaggttccc tcaccctaaa ggggatggtt ccccgccgcg
      241 tatcccgtca cactatgcgg caaccagccc gaagcctctc ggtccgagag gatgggcatc
      301 ccgcaaagga gggaacaatg ggcgacagcg tggtgaaggt gatcgagtta ctgtcggagt
      361 ccgacaagag ctgggaggac gccgcgcaac aggccgtgtc caaggccggg aaaagcctcc
      421 acggcatcaa gtcgatctac atcaagaatt tcgagggcaa agtcgaaggc aacaagatcg
      481 tgaagtatcg gatcaacgcg caggtgtcgt tccagctgga ctaggcttcc ggcgaacagg
      541 cggttcggtt gcggccctgt tgtttggcgt cggcgagcgc gcggttcgcg ctctccatga
      601 gggactcggg gccctccatg cccgtggaca gatcagccac cccgaggctg atggtgacgg
      661 acaggcgctg cccggcgaac atcaggtggc gcgtcgcaac gctcgtgcgt atgcgttcgg
      721 ccgccgccac cgcttcaacg gccgtggtgt gcggaaggat cagggccaat tcctcgcctc
      781 cgtagcgcgc cacaaggtcc gtgggccgca tcgccttctt gaacatcgcg gcgagttcct
      841 tgaccacgta gtcgccggcc ggctggctcc acgagtcatt gggcaggttg aacgggtcca
      901 cgctgatcag gatcacggag agcggctcct gcagactgag gcagcgcagc atgaggcgtt
      961 tgagatactc caggaacgtg cggcgattga tgaggccggt cagcgcgtcc acgcgctcgc
     1021 gatcgcccat cacgctgaca tagacgctct cggcgctgtt ggggcccagg tacttgagca
     1081 cggcccggcc gacccagatc acgtcgcctt catgcagcgc gcgttcggta attcgcttgt
     1141 cgttgcagaa cgtgccgttc cggctcccca ggtcccgcaa gatcgcggtc ccgtccggct
     1201 gggcgacgat ctccgcgtga tggcgcgaga ccaacgggtc gtcaaagcga atgtccgcgt
     1261 ccgcctgtcg cccgatcagc gtgcgcgcct ttgacagcgc gattttctct ccgatcgaca
     1321 gcccggcaat gaccaccagg cacgccagac tgcgttcctc ccgctccaac cgtcgaaggg
     1381 gagacacatc ggccatgccg atcgtggttt cgttggtttc ggaggggtct cgcagcggtt
     1441 cagggagcgc gttcatccgt cacaccacag attccggacg acaatagcat accggaggcg
     1501 gctgtcaacc tcggcccgcc gcgcgatgac gaccgtcccg gcatcttgcc gcccctgtcg
     1561 tccgatttcc tgataccatt taccgaagac gcggcgcagc cattggactg gatggacgag
     1621 atcgacgcaa aacccgaacc tgagtgggct tcctggcccg atgagcggtt gctcgacgcg
     1681 cgcctctgcg acctgaacct gtcgatcgac gggagcccgc tggtccagcg gatcgaccag
     1741 ttggcgaccg aattggccga ccgtcggatc gtcttccgtc cgcacgtgtg gatctcggac
     1801 gagtggttta cgccggacgg catccccggc attgcggtcc cgttttatct ggtgcatccc
     1861 cgactggtaa aactcgaact tcaacaaatg ctcgaagttg aaggcgggac ccacgagtgg
     1921 tgcatgcgga ttcttcggca cgaggccgga cacgcgatcg agaacgccta ccgcttgcgg
     1981 cttcgcacca ggcgcaaaca gctgttcggc cgatccaacg tcccgtatcc caacttctac
     2041 acgccgagac cgtacagcaa acggttcgtg ttgcacctgg acatgtggta cgcccagagc
     2101 cacccggacg aagacttcgc ggagacgttc gcggtctggc tcaatcccca gtcggattgg
     2161 ggcgcgcagt acgcgggctg gcccgcgctc aagaagctcg aatacatgga cgcgttgatg
     2221 cgcgacctcg cggacgtccc gcctccggtc acgcgcaccg accaggtcga tcccctgccg
     2281 tcactcacca agacgcttcg cgagcactac caggcgcggc gctctcacta cgggctcgaa
     2341 catccgcact tctacgatca ggatctccaa cggttgttcg tggaagcggc cgcggccccc
     2401 ggcgcgacga gcgcggcgca gttcatcgcg cgcatgcgca aggaagcccg gcgccgggtc
     2461 gcggatcgca ccggcgcgta ccaatacacc atcgaccggc tgctggaatc catgatcgcg
     2521 cgctgccgcg aactcgatct gaggctggcc aatgcgcccg aacaagctaa actggatttc
     2581 atgctgctgc tcaccgtgca gaccatgaac tttctccaca gcggccgtca tcgcgtcgcg
     2641 ttatgagcct gtccatgaac ctgagtgcac cgagggtgca cgtgttcatt ggcccgcagg
     2701 gaccctacag gtggcgcagc aaaggccatc tgccgcgttg tcggtcggcc ttgcgtgctc
     2761 acgtacgacc cagtacgctc cgcgcgcaag gctctctctc cgccttgcga ccaacctttg
     2821 aggatgcaaa tgacggaagt ccccgaaggg ggcatctggc cctccctgaa caggctccac
     2881 caaccggcga ctcgggcaag ctccgatgaa aaaactgcgc atcgcggccc tgatgcacga
     2941 agacttgatc ccgccggacg acctgggggg aatcgacctc gacgcggccg agtggaaaac
     3001 cgaatacgac gtggtcaagg cgttgcggtg catgaagcac gatgtgttgc cgatcggcgt
     3061 gcgcagcgat ttgggcctgc tcagaaaaac catcgccgac ttcaagcccg acatcgcgtt
     3121 caacctgctc gaagaattca acgacgtgcc gttgtgggac caaaacgtgg tctcgttcct
     3181 ggagctgatg ggcgtcccct acaccgggtg caacccgcgc ggcctcatgc tcgcgcgcga
     3241 caagggcctg tccaagaagc tgctctcgta ccatcggatt ccgttcccgg atttcatggt
     3301 cgtcccgatc gggcgcaagc cccgccgccc caaacgtctc gacttcccgt tgatcgtgaa
     3361 gtccgtgtgc gaggaggcct cgctcgggat ctcgcaggcc tcgatcgtga ccgacgacga
     3421 caagctgcgc gaacgcgtgg cgttcatgca cgagagcgtc aagacgcccg cgatcgcgga
     3481 gcgctacatc gcgggccgag aactgtacgt cggcgtgctg ggcaacgaga agctgcaggt
     3541 cctgccgatc tgggagctgg acttgagccg catgcccgag gacgcgccca agatcgccac
     3601 gcgccgcgtg aagtggagtc tcccgtatca gaagaagtac aacatcacgt ccggcgtggc
     3661 caaggacctg ccgaacggcc tgtccgatcg catccatcac ctggccaaac gcgtctaccg
     3721 cgttttgggc ttatccggct acgcccgcct ggacatgcgg ctcgacccgc agggccacgt
     3781 ctacatcatc gaagccaacc ccaaccccca gatcggccac ggcgaagact tcgccgaatc
     3841 cgcgaaagtc gtcggcctct cgtacaaggc cctgcttcaa cgcatcgtga cgctgggcct
     3901 ggcctggcgg ccggatagac tggcggcgta gaccacgcgg ccgaagacgc cgcgcgtgaa
     3961 atcggtcgcg gtcccacccg tcctgcggca acgcggcgga cgcgtttttt ttcaggagcc
     4021 cttagatttc ggctgaacag acggtccgat tccgcccttg ctgcttggcc gcgtacagcg
     4081 ccttatccgc ggcctgaagc agggcgtcgg cgctttcgat ctgagtcacc agatcagcca
     4141 ccccgaggct gatggtcacc gggataagcc gcccatcgaa cacgaagcgg tgcccggcca
     4201 cgatcgtacg catccgttcc gcgacgctca acgcctcgat caccgtggta tacggcacga
     4261 ggagcccgaa ttcctccccg ccgtagcgcg ccaggagatc cgtgggccgc accgcgcgct
     4321 tgagcagcga agccagttcc ttcaacaccg agtccccggc caagtggccc caggtatcgt
     4381 tgacgcgttt gaagtggtcg acgtcgaaga ggaccaccga catgggttcg cgcaggctcc
     4441 ggcaacgcga caccaggcgt tcgagatact cctggaacgt gcgccgattc acgagcgcgg
     4501 tcagtccgtc caggcgcgcg cgatcagcca tcgcgctcag gtacaggttt tccgcgctgt
     4561 ccggcgccag atacttgatg acggattggc cgatccacat cacgtcgcca tcgcgcagga
     4621 tgtgctcggt gatcggctga tcgttgcaca aggtgccgtt tcggctgttc aagtcccgca
     4681 acaccgaggt gccgtccggc cgcgtgatga tttccgcgtg gtgacgggag accagcgcat
     4741 cgtcgaggca gatgtccgaa tccgcctggc gtccgatgac catccggtct ttcgacaggg
     4801 cgatcttctc gccgatcgac agcccggcga cgacgaccaa gcaggcgaga ctgcgttcct
     4861 gccgattcaa ccggcgtttc ggagacgcat cagccatgcc gacggtcgtc tcgtcggtgt
     4921 cgcgagcaaa gggaatcggc ttgggattca cgctcatcgg gacgtccaaa cagaacgaca
     4981 ccacgatagc agcgccgcaa tacctgtcaa cgggaaaccg cgtctggagt agtacccgcc
     5041 cggcggttga cgcggacagc agctcccatt cacaacatcc cgacgcttca tctccacgac
     5101 gtcacaccct acaccctcgc ttcgggtctc agtttgtgat cggggctggc gctcgaccac
     5161 catcgttccg ctataatggg cggcttgcat gagccggaca tgatcgacgt cattgaactc
     5221 gtgattggtc tgttgattgg tgtggccgtg ctcgccgtgg tggcgaaccg gctgcaggtg
     5281 ccgaccgcca tcgtgttggt catcggcggc gcaatgttcg gactgattcc ggggctgccg
     5341 tccgtgcatc tggacccgga actcgtgttt ctgtttttct tgccgccgtt gctctatccc
     5401 gcggcgctgt acacgtcatg gcgagatttt cgggccaata tccggccgat ctcgttgttg
     5461 gccgtgggcc tggtgttgtt caccaccctc gtgatcgcgt ggttggcgca ccggttcatg
     5521 ggattgccgc tcgcggcggg cttcgtgctc ggcgccatcg tctccccgcc cgacgcggtc
     5581 gcggccacgg ccattaccca gcggttgcgc gtgccccgcc gcatcatcac catcctggaa
     5641 ggcgagagcc tggtgaacga cgccaccgcg ttggtggcct accggtttgc cgtggccgcc
     5701 gtagtcacgg ggatgttttc gtggaccgac gcgggctggc gattcctggt ggccggggtc
     5761 ggcggcattg cgtttggatg gggtgtgggc cgtgtggcgg catgggttca gcagcggctc
     5821 gaagacccgg cgatcaaagt cacggtgtcg ctgttgacgc cttttgccgc ctacgtgccc
     5881 gctgatcggc tcggtgtttc cggcatcctc gccgtggtca cggccggcgt gtatctcgga
     5941 tggcgctctc cggaaatcac caacgcgtcc atgcgcctgc aggtccggcc ggtgtggtcc
     6001 actatcacct tcttgctgaa cgggtttgtc ttcatcctca tcggcctgca actctccgag
     6061 attctggata gcctgtcggg ctacactgcc ggacagctcg cctggtatgc caccctcatc
     6121 agcctcgcga cgatcctggt gcggatgatc tgggtgttcc cggccgcata tctgccgcgt
     6181 tggctcttca aaagtatcc
//
LOCUS       DBNGTT010000049         3210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3459228, whole genome shotgun sequence.
ACCESSION   DBNGTT010000049 DBNGTT010000000
VERSION     DBNGTT010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3459228"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..926
                     /locus_tag="AC16BC_01130"
     CDS             72..926
                     /locus_tag="AC16BC_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3240242.1"
                     /translation="MSGRYGAGGLGEVRPGVIASSRWADDAAGVYTIWLREVRKFWRD
                     KPRIIGATVQPALFLLLLGTGIGKGIPHAFNVEPSGREFAPGVPDYLTFIYPGIIAMT
                     LLFTSVFSAISIIWDREIGFLKEVMVAPISRAAVAVGKTLGGSTVAMLQGCLMLLFAP
                     FLHVQFSWASALALLPLLLYISVSLTAMGIAAASRMRSMQGFTVIMNFLTLPMFFLSG
                     AMFPVDGLPHWMAVLVSLNPLTYGVDALRSVIIGFHRFGVIQDLVVMGVFGVAMLALA
                     VRGFESRD"
     gene            919..>3210
                     /locus_tag="AC16BC_01135"
     CDS             919..>3210
                     /locus_tag="AC16BC_01135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3240243.1"
                     /translation="MTDAGRTRRRARPLIGFRGTLAAALLAAGLAPLTVAVAVVWSAR
                     RGIAEWPGWAGVALYAALAALTAALAAAGSRALARPVQALRDGVRRIGEGDLDHRLDI
                     PAERDLAQLAAAFNAMAGRLAESYRTLERTVADRTMALTAVNTVAVTANRSLDPDEIL
                     GSVLDRLPVVLNLEAAFIRLLDERGLTLRAHRGVPAGALEAMAVCPMGEGFSGRVAAR
                     GTPLVADAATDGMGDAGEAALFAQGFRAAACVPVMAKGHVLGTLAVASRYPRTFTPQD
                     LNLLTSIGSQVGTALENAALYERACAMVKEFETLDRLKTEFLSNVGHELRIPLTAIIG
                     FAELLLERIPGELTVEQDRYVRIMLDSGRDLLGMINNLLDLSKIKAGKLDWRAAPFDV
                     EPLVALVADTIRPLIVKQRLHLAVEVEDGPVMAYADEGLVKQVLLNLLSNAVKFTPAG
                     GSVTLSVRAAADGPEHGAVELRVEDTGIGIAPENLERIFQEFHQVDGSPTRDHPGTGL
                     GLAIAKRLVELQGGKIWAESRVGQGSRFVVWLPRGRAAESAPVPTRQAGIMESVMESL
                     VATAAARSIEARRTPKIAVVGSTWIAEQVRKNVEADGYDAQAMAPDDVSTLRARPTRP
                     FAVVVEAHDAAPARAALSALRTLVDVPIVWLAVSDDGMRACAPAAIGVVASPPAPDAV
                     RALLRGCGLLRAVKRRPTTVLVVADHAVTEALQEGLRPDGVGVQRAAPEDAATAAGEL
                     GPDLLLVDASEADPFALAQRLRQH"
BASE COUNT          415 a         1042 c         1211 g          542 t
ORIGIN      
        1 agccgcccgc tcacgccccc ctccgcccgc cgtcgccttc gctgcgcaga tgccggccgg
       61 tggagggggg cgtgagcggg cggtacggtg cgggcgggct gggtgaggtg cggcccggcg
      121 tgatcgcgtc cagccgctgg gcggacgacg ccgcgggcgt gtacaccatc tggctgcggg
      181 aagtccgcaa gttctggcgc gacaagccgc gcatcatcgg cgccacggtg cagcccgcgc
      241 tctttctgtt gttgctcggg accggcatcg gcaagggcat tccgcacgcg ttcaacgtcg
      301 agccgtcggg ccgcgagttc gcgccgggcg tgccggatta cctgaccttc atctatcccg
      361 gcatcatcgc gatgacgttg ctgtttacgt cggtgttttc ggccatctcg atcatctggg
      421 atcgggagat cggatttctc aaggaagtga tggtggcgcc catttctcgc gccgcggtcg
      481 cggtgggaaa gaccttgggc gggagcacgg tggccatgct ccaagggtgt ttgatgctgc
      541 tgttcgcgcc ctttctgcac gtgcagtttt cgtgggcgtc cgcgctcgcg ctgttgccgt
      601 tgctcctgta catctcggtg tcgctcaccg cgatggggat cgcggccgcc tcgcgcatgc
      661 ggtccatgca ggggttcacc gtgatcatga attttctgac gctgcccatg ttttttctgt
      721 ccggcgcgat gttcccggtc gacggcctgc cccactggat ggccgtgctc gtgagtctca
      781 acccgttgac ctacggcgtg gacgccctgc ggtccgtcat catcggtttt caccggttcg
      841 gcgtgatcca agacctggtg gtgatgggcg tgttcggggt cgcgatgctc gcgctcgcgg
      901 tgcgggggtt cgagtcccgt gactgacgcc ggtcgcaccc gtcggcgcgc gcgcccgctg
      961 atcgggtttc gcggcacgct cgccgccgca ctcctggccg cgggcctggc gcccttgacc
     1021 gtggcggtgg ccgtggtgtg gtcggcgcgc cgcgggatcg ccgagtggcc ggggtgggcc
     1081 ggggtcgcgc tgtacgcggc gttggccgcg ttgacggccg cgctcgcggc ggcgggctcg
     1141 cgcgcgctgg cccggccggt ccaggcgttg cgcgacggcg tgcggcgcat cggggaaggg
     1201 gatctggatc accggctcga catcccggcc gagcgcgatt tagcccaact cgccgcggcg
     1261 ttcaacgcca tggcgggccg gctggcggag tcctaccgca cgctcgaacg gaccgtcgcg
     1321 gaccgcacca tggcgctgac cgcggtgaac acggtggcgg tgaccgcgaa ccgctcgctg
     1381 gatcccgacg agatcctggg gagcgtgctg gatcgcctgc ccgtggtgtt gaatctggag
     1441 gcggcgttca tccggttgct cgacgaacgc gggttgacgt tgcgcgcgca ccgcggcgtg
     1501 cccgcgggcg cgctggaagc catggccgtg tgcccgatgg gcgagggctt ctccggccgc
     1561 gtggccgcgc gcgggacccc gctcgtggcc gacgcggcga ccgacggcat gggcgacgcc
     1621 ggggaggccg cgctgttcgc gcaggggttt cgcgccgccg cgtgcgtgcc ggtcatggcc
     1681 aagggacatg tgctggggac gttggccgtg gccagtcggt atccgcgaac gttcacgccg
     1741 caggatctca atctcctcac ctcgatcggg agtcaggtcg ggaccgcgct cgaaaacgcc
     1801 gcgctctacg agcgcgcctg cgccatggtc aaggagttcg aaaccctcga ccgcctcaag
     1861 accgagtttc tctccaacgt cgggcacgaa ctgcggattc cgctgaccgc gatcatcggg
     1921 ttcgccgagt tgttgttgga gcggattccg ggcgaactca ccgtggaaca ggaccgttac
     1981 gtgcgcatca tgctcgacag cgggcgcgac ctgctcggca tgatcaacaa tctgctcgac
     2041 ctgtccaaga tcaaggccgg caagctggat tggcgcgccg ccccgttcga cgtggaaccg
     2101 ctcgtggcgc tggtggcgga tacgatccgg ccgttgatcg tcaagcagcg cctgcacctg
     2161 gcggtcgagg ttgaggacgg tccggtcatg gcgtacgccg atgaggggtt ggtcaaacag
     2221 gtgctcctga atctgttgtc caacgcggtg aagttcacgc ccgcgggcgg gtccgtcacg
     2281 ctcagcgtgc gggccgcggc cgacggcccg gaacacgggg cggtggaact gcgcgtcgag
     2341 gacaccggca tcgggatcgc gcccgagaat ctggagcgga tcttccagga gttccaccag
     2401 gtggacggca gtcccaccag ggaccatccc ggcaccggat tggggttggc catcgcgaaa
     2461 cggctggtgg agctgcaggg cgggaagatc tgggcggaga gccgggtggg gcagggcagc
     2521 cggttcgtgg tgtggttgcc gcgcgggcgc gccgcggaat cggctccggt tccgacgcgc
     2581 caggccggaa tcatggaatc ggtcatggag tcgttggtgg ccacggccgc ggcgcgctcg
     2641 atcgaagcgc ggcgaacgcc caagatcgcg gtggtgggat cgacgtggat cgcggagcag
     2701 gtccgcaaga acgtggaagc agacggatac gacgcgcagg ccatggcgcc ggacgacgtc
     2761 tcgaccctgc gcgcgcggcc cacgcggccg ttcgcggtgg tggtcgaggc gcatgacgcc
     2821 gcgccggcgc gcgcggcgtt gagcgcgctg cggaccctgg tcgacgtgcc gatcgtgtgg
     2881 ctcgcggtct cggacgacgg gatgcgcgcg tgcgcgcccg ccgccatcgg cgtggtggcg
     2941 tccccgcccg cgcccgacgc ggtgcgcgcc ctcttgcgcg gatgcgggct cctgcgcgcg
     3001 gtcaaacgcc ggcccaccac ggtgctggtg gtggccgatc acgccgtgac cgaggcgttg
     3061 caggaggggt tgcggccgga cggcgtgggc gtgcagcgcg ccgcgcccga agacgcggcg
     3121 accgcagccg gagagctggg cccggatctg ttgctggtcg acgcgagcga ggccgatccg
     3181 ttcgcgctcg cgcaacgcct gaggcaacac
//
LOCUS       DBNGTT010000050         1619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1159974, whole genome shotgun sequence.
ACCESSION   DBNGTT010000050 DBNGTT010000000
VERSION     DBNGTT010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1159974"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..345
                     /locus_tag="AC16BC_01140"
     CDS             109..345
                     /locus_tag="AC16BC_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HIR3240244.1"
                     /translation="MLEGDVTRIQHLRNTNHEFVALERRHDALEHELDDLMRRRILTP
                     SDEIRKKTVQKQKLVMKDRMYDMLRRSSPAEAAS"
     gene            368..598
                     /gene="tatB"
                     /locus_tag="AC16BC_01145"
     CDS             368..598
                     /gene="tatB"
                     /locus_tag="AC16BC_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR3240245.1"
                     /translation="MFGIGFPELIVILLIALIVLGPQRLPELARTLGQWFAAIKQAAD
                     DAQTQVRAELDAAERSVEHPEPAPPPAQKSDG"
     gene            591..1340
                     /gene="tatC"
                     /locus_tag="AC16BC_01150"
     CDS             591..1340
                     /gene="tatC"
                     /locus_tag="AC16BC_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3240246.1"
                     /translation="MAEQESGGRMSLFEHLAELRRRLLISVAAVLVVAVATFSFSSDI
                     LAWLTAPLETELVFLSPTEAFWVSFKISLVVGLLVSLPLVLYQIWRFVAPGLYRTERR
                     YAAAFVIGSCAFFAAGLAFCAFVALPFALKFLIAFGVDRGMKPMISAQMYVDFALKFY
                     LAFGLIFEVPLAVTLAGRMGLVTPAFLARNRRYALLVNAILAAVLTPTSDLFNMAIML
                     VPLTVLYEIGIIGARIFGRRRPAPEAAAAGV"
     gene            1347..>1619
                     /locus_tag="AC16BC_01155"
     CDS             1347..>1619
                     /locus_tag="AC16BC_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR3240247.1"
                     /translation="MPELPEVESIVRRLRPALEARTIESVKLLRRDIWTGDASVDDLP
                     KRSIASVRRHGKSILIETSSPPLWLVVRLGMTGQCLIVPRGAERAPH"
BASE COUNT          256 a          506 c          538 g          319 t
ORIGIN      
        1 cgtgcgggcc ggttattacc acgatccgaa agaagacgcg ttgctgatgg aatgggcgcg
       61 gtgaggggag ggttccaacc ggggcgatgt caacaaggag gaatcgcgat gctcgaaggg
      121 gatgtcacgc ggatccagca cctccggaac acgaaccacg aattcgtcgc gcttgagcga
      181 cggcacgatg ccttggagca tgagttggac gatttgatga gacggcgaat tctcacgccc
      241 agcgacgaga ttcggaagaa gaccgtgcag aaacagaaac tcgtgatgaa ggatcggatg
      301 tacgatatgt tgcgccgcag ttccccggcc gaggccgcgt cgtaactcgc gttcgggccc
      361 gttcggtatg ttcggcatcg gctttccgga actgatcgtc atcctgttga tcgcgttgat
      421 cgtgctgggg ccccaacggc ttccggaact ggcgcggacg ttgggccaat ggttcgccgc
      481 gatcaagcag gcggccgacg acgcgcagac gcaggtgcgg gcggaactcg acgcggcgga
      541 acggagcgtg gagcacccgg aacccgcccc gccacccgcg caaaagtccg atggctgagc
      601 aggaatccgg cggacgcatg tcgctgttcg aacacctggc ggaactgcgc cgtcggcttt
      661 tgatcagcgt ggccgccgtg ctggtcgtgg ccgtcgcgac cttctccttc tcgtccgaca
      721 tcctggcgtg gctgaccgcg ccgcttgaga ccgaactggt ctttctgtcc ccgaccgaag
      781 cgttctgggt ttctttcaag atctcgctcg tggtgggcct gctcgtctcc ctgcccctgg
      841 tgctctatca aatctggcgc ttcgtggcgc cggggttgta tcggacggaa cggcgctacg
      901 ccgccgcgtt cgtgatcggg tcgtgcgcgt tcttcgcggc gggcctcgcg ttttgcgcgt
      961 tcgtggcgct gccgttcgcg ctcaagtttc tgatcgcgtt cggcgtggat cgcggcatga
     1021 agcccatgat ctccgcgcag atgtacgtgg atttcgcgct caagttctac ctggcgttcg
     1081 gcctgatttt cgaggtcccg ctcgccgtga ccctggccgg caggatgggg ctggtcacgc
     1141 ccgcgttcct cgcgcgcaac cgacgctacg ccctgctggt caacgcgatc ctcgccgccg
     1201 tgctcacgcc cacctccgac ctgttcaaca tggccatcat gctggtgccg ctgaccgtgt
     1261 tgtacgagat cgggatcatc ggggcgagaa ttttcggccg gcggcggccc gcgccggaag
     1321 ccgccgcggc cggcgtatag gcaactgtgc ctgaactgcc tgaagtggag tccatcgtgc
     1381 ggcgcctccg tcccgcgttg gaagcgcgca cgatcgaatc cgtcaaactg ttgcgtcgcg
     1441 atatctggac cggggacgcg tcggtcgacg atctgcccaa acggtcgatc gcgagtgtcc
     1501 gccgtcacgg caaatccatc ttgatcgaga cgtcgtcccc gccgctctgg ctggtggttc
     1561 gcctgggaat gaccgggcaa tgcttgatcg tgccccgcgg ggcggagcgc gcgccgcac
//
LOCUS       DBNGTT010000051         3866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_649178, whole genome shotgun sequence.
ACCESSION   DBNGTT010000051 DBNGTT010000000
VERSION     DBNGTT010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_649178"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="AC16BC_01160"
     CDS             <1..1576
                     /locus_tag="AC16BC_01160"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR3240248.1"
                     /translation="AAIPQRSMTGESPQEPYDLIVIGGGVVGSWIACDAAQRGLRAAL
                     FEQGDVASGTSSRTSKLIHGGLRYLEQGSFRLVAEGTRERAVWMRIAPHLVRPLPFLF
                     PLYRDTGRSAWVVRLGMTLYDMLSLYRNIEPHRMVSTEEAVRQEPALRHPGLAGAARF
                     FDAQMDDARACLEVALSAAAAGARVETYARVDALLRRNGRVAGVRVGSRDVFARVVVN
                     AAGPWLDRVSAMADGAAHRIRMTRGAHLVLPPLLRQHALVLTARQDGRVFFVMPWRGH
                     TLVGTTDLDYTGDPAAVTCTSGERQYLLAEVRHALPDVGIQDADVVAEFAGVRPLAFE
                     AGGSASSVSREDLIAEDPSGVVAIAGGKFTTARAVAERAVTRIAGRIDRIALAPCRTD
                     STPLVGGRPVGAAQAQSWQARVRALGLSEAQYGALLDMYGSRLGLLLDLMAQRGTGGR
                     LHPDLPWVGAQVDFAVEHELARTVEDVLRRRLPIALGPHRRDGSITRAVVERMGALLG
                     WDAVARDDAVSRYLAA"
     gene            1631..1939
                     /locus_tag="AC16BC_01165"
     CDS             1631..1939
                     /locus_tag="AC16BC_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HIR3240249.1"
                     /translation="MVLLEFSMSPLDKGESVGQYVARSLDIVDKSGVSYRLNPMGTVL
                     EGEWDEVFGVVKKCYERMRKDCRRISVGIRVDYRKGAKGRLESKIASVERRLKRKVRT
                     "
     gene            2043..3608
                     /locus_tag="AC16BC_01170"
     CDS             2043..3608
                     /locus_tag="AC16BC_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240250.1"
                     /translation="MPPVGSRLGRPAVLSVSGVPPLIPLTSSVSETLTVPSASTSVAA
                     SARRLGSLRTQGLFAAADGDGLPQWRVSVDPFWLTPDQVSFLERLGPTLLAFYQSLNR
                     LYYHSLKTPALRWVSDYLDQGKPQPLVDFGRMRRVRDQLPCLIRPDLFVTDQGWIASE
                     LDAVPGGFGLTAALQELYGDASLVGGSDGIVRGLVSAFGSDSRVAIVVSDESADYRPE
                     MRFLARALSAAGPPARAVAPADLVFQESGLVIDLPQGPERVDAVYRFFELFDLKNVPK
                     SELFLYAAKKGTVRMTPPAKPFLEEKLAMALWHHPALRTWWRDDVGPSTADALTALFP
                     RTWILDPSPVPPHATIAGFVLDKGPVRSWLDIADATQRGRRFVIKPSGFSPLAWGGRG
                     IVVGHDQPAAAWRHALESALAAFPATPHVLQEFHTARRDEVAYFDEPSGATRSTAGRT
                     RLSPYYVVSGDQVTLGGVLATTCPLDKKIIHGMRDAVLAPGAVLTPAVGRVGRAVGLP
                     TATAFVTDTRSQT"
     gene            3605..>3866
                     /locus_tag="AC16BC_01175"
     CDS             3605..>3866
                     /locus_tag="AC16BC_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240251.1"
                     /translation="MSDQWKYDIRDSLEAVEDLIGRLGLTPHEAERLREVVARYPMRI
                     TPQVLSTIRYKGDPIWLQVVPTTDELDLNLDMQDDPLNEEGDM"
BASE COUNT          585 a         1207 c         1393 g          681 t
ORIGIN      
        1 ggcagcaatt ccgcagcgga gtatgacggg cgaatcgccg caagaaccgt acgacctgat
       61 cgtgatcggc ggaggcgtgg tgggctcctg gatcgcctgc gacgcggctc agcggggact
      121 gcgcgccgcc ttgttcgaac agggcgacgt ggcgagcggc accagcagcc ggacgtcgaa
      181 actgatccac ggcggcctgc ggtacctgga gcagggctcg ttccggctgg tggccgaggg
      241 cacgcgcgaa cgcgcggtgt ggatgcggat cgcgccgcac ctggtgcggc ccctgccctt
      301 tctgtttccg ctctatcgcg atacggggcg ttccgcctgg gtcgtccgac tcggcatgac
      361 cctgtacgac atgttgtccc tgtatcgcaa catcgaaccc caccgcatgg tctcgacgga
      421 ggaggccgtc cggcaagagc cggccctgcg tcatccgggg ctggccggcg ccgcgcggtt
      481 cttcgacgcg caaatggacg atgcccgcgc ctgtctggaa gtggcgctgt cggcggccgc
      541 ggccggagcg cgcgtggaaa cttacgcgcg cgtggacgcc ctcctccgcc gcaacgggcg
      601 cgtcgcgggc gtgcgcgtcg gctcgagaga cgtgttcgcc cgcgtggtgg tcaatgccgc
      661 cggaccgtgg ctggatcgcg tgagcgcgat ggccgatggc gcggcgcatc ggattcgcat
      721 gacgcgcggc gcgcatctgg tgttgccgcc gctattgcgc cagcacgcgc tggtcctcac
      781 ggcgcggcag gacggccgcg tgttcttcgt gatgccctgg cgtggccata ccctggtcgg
      841 cacgacggac ctggattaca caggggaccc cgcggcggtg acgtgcacga gcggcgagcg
      901 acagtacttg ctggcggagg tccgtcatgc gttgccggac gtcgggattc aggacgccga
      961 cgtggtggcg gaattcgcgg gagtgcgccc cctggccttt gaggcgggcg gttccgcgtc
     1021 ctcggtgagc cgggaagatc tcattgccga agacccctcc ggcgtggtgg cgatcgcggg
     1081 cggcaagttc acgaccgcgc gggccgtggc ggagcgcgcg gtgacgcgaa tcgccgggag
     1141 aatcgaccga atcgcgctcg ctccgtgccg caccgactcg accccgttgg tgggcgggcg
     1201 tcccgtgggc gcggcccagg cgcagtcatg gcaagcccgc gttcgagcgc tcggattgtc
     1261 ggaggcgcag tacggcgcgt tgctcgatat gtacggctcg cggctcggtc tgctgctgga
     1321 tctgatggcc caacgcggca ccgggggacg cttgcacccg gacctcccct gggtcggggc
     1381 gcaggtggat tttgccgtgg aacacgagtt ggcccgcacc gtggaagacg tgttgcgtcg
     1441 acggttgccg atcgcgctcg ggccccaccg ccgcgacgga tcgatcaccc gcgccgtcgt
     1501 cgagcgcatg ggcgcgctcc tgggctggga cgcggtcgcg cgcgacgacg cggtctcgcg
     1561 gtatctggcg gcgtaaccgg tcgttcgtgt tcacgcgaac aacatcgtct cgcacaagga
     1621 cggaggcacc atggtacttc tagaattcag catgtccccg ctggacaagg gcgaaagtgt
     1681 cgggcaatac gtggcgcgtt cattggacat cgtcgacaag agcggcgtgt cataccggtt
     1741 gaacccgatg ggcacggtcc tggaaggcga atgggacgag gtattcgggg tggtcaagaa
     1801 atgttacgag cgcatgcgca aggactgccg gcggatctcg gtgggcatcc gcgtggatta
     1861 ccgcaagggc gccaagggcc ggctcgaatc caaaatcgcc agcgtggagc ggcggctcaa
     1921 acgcaaggtc cggacgtaac gtccgcgcgt cgcggcgttg cttgacgacg gcgatcagcc
     1981 ccatggtaag ataccgacca tgtcggacga tggctgttgt catcgaaccg ggctgatcgt
     2041 cagtgccgcc ggtcgggtcg cggctcggcc ggccggccgt tctctcggtg agcggcgttc
     2101 cgccgctgat cccgttgacc tcctcggtta gcgagacgct cacggttccg tctgcgtcga
     2161 ccagcgttgc ggcgagtgcg cggcgcctgg gttcgctgcg cacgcagggc cttttcgcgg
     2221 ccgcggacgg tgacggtctg ccgcagtggc gggtcagtgt tgacccgttc tggctcacgc
     2281 ccgaccaggt ctcgttcttg gagcgtctgg gcccgaccct cctggcgttc tatcaatcgc
     2341 tcaatcggct ctactatcac agcctgaaaa ctccggcgct gcggtgggta tcggactatc
     2401 tcgatcaagg caaaccgcaa cccctggtcg acttcggacg gatgcggcgc gtccgcgatc
     2461 aactgccgtg tctcatccgg ccggacctgt tcgtcacgga ccagggctgg atcgcgtcgg
     2521 aattggacgc ggttcccggg ggattcgggc tgaccgcggc gctccaggaa ctgtacggcg
     2581 acgcgtcgct ggtcggcggc agcgacggca ttgtgcgcgg gttggtgagc gcgttcggtt
     2641 cggactcgcg cgtcgcgatc gtggtgtccg acgagtcggc ggactatcga ccggaaatgc
     2701 ggttcctcgc gagggcgttg tcggccgccg gtccgccggc gcgggcggtc gcgccggcgg
     2761 accttgtgtt tcaggagagc ggtttggtca tcgacctgcc tcaagggccg gaacgggttg
     2821 atgccgtgta tcgattcttt gagttgtttg atctcaaaaa cgtgccgaaa tccgagttgt
     2881 tcctgtacgc ggccaagaaa ggcacggtcc gaatgacccc gccagccaag ccgtttttgg
     2941 aagagaagct ggccatggcg ttgtggcacc acccggcgtt gcgaacctgg tggcgagacg
     3001 acgtggggcc gtcgaccgcg gacgcgctca ccgcgttgtt tccgaggacc tggatcttgg
     3061 acccttcccc ggtgcctccg cacgcgacga tcgccgggtt cgtcctggac aaagggccgg
     3121 tccgatcgtg gctcgatatc gcggacgcga cccagcgcgg gcggcgattc gtgatcaagc
     3181 cgtcggggtt ctcgcccctg gcgtggggcg ggcgggggat agtggtgggc cacgatcaac
     3241 cggcggccgc gtggcgacac gcgctggagt cggcattggc ggcatttccc gcgacgccgc
     3301 acgtgctcca ggaattccac acggcgcgcc gggacgaggt cgcgtatttc gacgagccgt
     3361 cgggcgcgac gcgctcgacg gccggtcgca cgcgcctctc gccgtattat gtggtgtcgg
     3421 gcgaccaggt gaccttgggc ggcgtgttgg ccacgacctg cccattggac aagaagatta
     3481 tccacgggat gcgggacgcg gtgctcgcgc ccggcgctgt tttgaccccg gctgtcggcc
     3541 gagtcggacg ggcggtaggc cttcccaccg cgacggcgtt tgtgactgac acgaggagcc
     3601 agacgtgagc gatcagtgga aatacgatat acgcgacagt cttgaggccg tggaagatct
     3661 catcggacga ctcgggctca cgccgcacga ggccgaacga ttgcgcgagg tggtggcgcg
     3721 ttacccgatg cggatcaccc cgcaggtctt gagcacgatt cgctacaaag gcgatccgat
     3781 ttggctgcaa gtggttccca ccaccgatga actcgacctg aatctcgata tgcaggacga
     3841 ccccctcaat gaagagggcg atatgg
//
LOCUS       DBNGTT010000052         3641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2437583, whole genome shotgun sequence.
ACCESSION   DBNGTT010000052 DBNGTT010000000
VERSION     DBNGTT010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2437583"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="AC16BC_01180"
     CDS             <1..170
                     /locus_tag="AC16BC_01180"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR3240252.1"
                     /translation="LALDRLGLAPVLDLGLRLGEGTGAALAIGLLDAAVKVLNEMATF
                     GEAGVSERVAP"
     gene            170..922
                     /gene="cobS"
                     /locus_tag="AC16BC_01185"
     CDS             170..922
                     /gene="cobS"
                     /locus_tag="AC16BC_01185"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR3240253.1"
                     /translation="MDRVLVAWEFLTVVPLRRRGRAADEGACGRSLAAFPLVGLALGA
                     ALAAFSLAAVLLFPPALVAALAVVLLAWLSGGLHLDGVADTADACGARSREDALRIMK
                     SSTIGTYGSLALFSVLGLKIAALATLPPRALAPALLAMPVLGRWAQVHATVGRTYARE
                     QGTAKAFVEGATRAAWWVATVTTLLIAAAVMGPAGLAAVAVVWAAVTVYARRVTAWLG
                     GVTGDTIGSAGEGAEVLAVLTWAAFFHVRGAA"
     gene            928..2382
                     /locus_tag="AC16BC_01190"
     CDS             928..2382
                     /locus_tag="AC16BC_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR3240254.1"
                     /translation="MIQGTGSNVGKSLVTAALCRIFLQDGYRVAPFKSQNMALNAAVT
                     RDGLEMSRAQALQAQACRIEPDARMNPILLKPSSEDGCQVVVMGKVRGTLSADAYLDA
                     KAELFPVVKDAYASLAAEHEVIVIEGAGSPAEINLMDREIVNMRVADMADAPVLLVGD
                     IDSGGVFAALVGTLALLPDAHAARVKGLLINKFRGRRSLLDPGLDAVARRTGRLVLGV
                     IPYLDRLRLPDEDSLALRSRAAEVDSSSSVTVAVIELPRISNFNDVDPLRVEPDVCVR
                     FVRPGERLGRPDVVILPGSKNAATDLVALRACGLAEELRAHAASGGAVIGLCGGLQMM
                     GRTIEDPGAIESRVDRTEGLGLLPIETVLEDEKILRRVAGRHRSTGCEVTGYEIHHGR
                     SRRLNVGEPLFVLDDGRADGVARGACWGTYLHGVFDGDGFRRAMVDSWRTGKGLAPLG
                     TVQARYDVDAELDRLAAAVRAAVDLDAVYRVMGR"
     gene            2397..3347
                     /gene="cbiB"
                     /locus_tag="AC16BC_01195"
     CDS             2397..3347
                     /gene="cbiB"
                     /locus_tag="AC16BC_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR3240255.1"
                     /translation="MEIVLLVFGALALDAVLGDPRGLPHPVRGLGRIIASLERALRAH
                     YPLRERAAGVALALGVVGGTFAAAWGVIHLAGLIHPWAARGTEVLAIYWSLAPRDLWQ
                     HAARVSGALGRGDLDEAKRGVAMMVGRDVEPLDEKGVTRATIESVAENTVDGVTAPLC
                     FALLGGGPLAMAYRAVNTLDSMVGYRIEPYARIGWASARLDDLANLIPARLTALLMPL
                     AGGFLGGSVRGGWSAAWRDGRKHDSPNSGLTEAAMAGVLGIELGGPHWYRGTYYASPT
                     IGTARRPVERADITWSCRVMAVTTALVFVIGASARLWIDP"
     gene            3344..>3641
                     /locus_tag="AC16BC_01200"
     CDS             3344..>3641
                     /locus_tag="AC16BC_01200"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3240256.1"
                     /translation="MNDLNGLNDLNDLNRNSHGLVLVYTGDGKGKTTAAFGLALRAAG
                     RGLRVHVIQFIKGEMDTGEIEMIAKALPSITVERVGKGFTWRRDVVPTFEDHAAA"
BASE COUNT          467 a         1145 c         1423 g          606 t
ORIGIN      
        1 acctggcgct cgatcggttg gggctcgcgc cggtcttgga tctggggctt cgcctcggcg
       61 agggtaccgg cgcggccctg gcgatcgggc tgctcgatgc ggcggtgaag gtccttaacg
      121 agatggcgac cttcggcgaa gccggcgtct cggagcgcgt cgcgccatga tggaccgcgt
      181 gctggtcgcg tgggagtttc tgaccgtggt cccgctgcgc cgccgagggc gcgccgcgga
      241 cgagggagca tgcgggcgct cgctcgccgc gtttcccctc gtggggctcg cgctcggcgc
      301 ggcgctggcg gcgttctcgc tggcggccgt cctgctgttt ccccccgcgc tggtggcggc
      361 gctcgccgtc gtgcttttgg cgtggctctc gggcggcctg cacctggacg gcgtcgcgga
      421 taccgccgac gcatgcggcg cgcgctcgcg cgaggacgcg ttgcggatca tgaagagctc
      481 gacgatcggg acctacggga gcctggccct gttctcggtg ctggggctga agatcgcggc
      541 gctggcaacg ctgccgcccc gtgcgctcgc acccgcgctg ctcgccatgc cggtactggg
      601 gcgctgggcc caggtccacg cgacggtcgg acggacgtac gcgcgcgagc aggggaccgc
      661 caaggcattc gtcgagggtg cgacccgcgc ggcctggtgg gtggcgacgg tgacgacgct
      721 cctcatcgcg gccgcggtca tgggaccggc ggggctcgcg gcggtcgcgg tcgtgtgggc
      781 cgcggtgacc gtgtatgccc gccgggtcac ggcgtggctg ggcggcgtga ccggcgacac
      841 gatcggctcc gcgggcgagg gcgcggaagt gctcgcggtc ctgacctggg cggcgttctt
      901 tcacgtgcgg ggtgcggcgt gaacctgatg attcaaggga ccggttctaa tgtcggcaaa
      961 agcctcgtga cagccgcgct gtgccggatt tttctccagg acggctatcg cgtcgccccg
     1021 ttcaagtccc agaacatggc gttgaacgcg gccgtcaccc gcgacggcct ggagatgagc
     1081 cgcgcccagg cgctccaggc ccaggcctgc cggatcgagc ccgacgcgcg gatgaacccc
     1141 attttgctca agccctcgtc ggaggacggc tgccaagtgg tcgtgatggg caaggtccgc
     1201 ggcacgctgt ccgccgacgc gtacctggac gccaaggccg agctattccc ggtcgtgaag
     1261 gacgcatacg cctcgctcgc ggccgagcac gaggtgatcg tgatcgaggg cgccggaagc
     1321 cccgccgaga tcaatctcat ggaccgcgag atcgtgaaca tgcgggtggc cgacatggcc
     1381 gacgcgcccg tgctcttagt cggcgacatc gactcgggtg gcgtcttcgc ggcgctggtg
     1441 ggcacgctgg cgctcctgcc ggacgcacac gccgcgcggg tgaaaggact cttgatcaat
     1501 aaattccgcg gccgccggtc gctgctcgat cccggcctcg acgcggtggc acgccgcacc
     1561 ggccgcctgg tgctcggcgt gatcccatac ctggatcgcc tgcgcctgcc cgacgaggac
     1621 tcgctggcgc tccgctcgcg cgcggcggag gtcgattcct cgtcatcggt gacggtcgcg
     1681 gtgatcgagc tcccgcgcat cagcaacttc aacgacgtcg atccgctgcg agtcgagccg
     1741 gacgtttgcg tgcggttcgt gcgaccaggc gagcggttgg gccgccccga cgtggtgatc
     1801 cttccggggt ccaagaatgc ggcgaccgat ctggtggcgt tgcgtgcgtg cggcctggcc
     1861 gaggagcttc gcgcccacgc cgcgtcgggg ggtgccgtga tcgggctttg cggcgggctg
     1921 cagatgatgg gccggacgat cgaagacccc ggcgccattg aaagccgggt ggaccgcacc
     1981 gagggcctgg ggctgctgcc catcgagacc gtcttggagg atgagaagat tctgcggcgc
     2041 gtcgcggggc gtcaccggtc cacggggtgc gaagtgacgg gctacgagat ccaccacggc
     2101 cgaagccgcc gcctcaatgt gggcgagccg ctgtttgtcc tcgatgacgg tcgtgccgac
     2161 ggcgtcgcgc gcggggcgtg ctgggggacc tatctccacg gggtcttcga cggcgacggg
     2221 ttccggcggg cgatggtcga ttcgtggcgg acggggaagg ggctggcgcc gctggggacc
     2281 gtccaggccc gctacgacgt ggacgccgaa ctggatcggc tggcggcggc ggtgcgggcg
     2341 gcggtggatc tcgacgcggt gtaccgggta atggggcggt gaccggcccg gaccacatgg
     2401 agatcgttct cctcgtcttc ggcgcgttgg cgctcgacgc ggtgctgggc gatccgcgcg
     2461 gattgccgca tcccgtgcgc ggtttgggcc ggatcatcgc gagcttggag cgcgcgttgc
     2521 gcgcgcacta tccgttgcgc gagcgggcgg ccggggttgc gttggcgctc ggtgtggtcg
     2581 gggggacatt tgcggcagcc tggggtgtga tccacctggc ggggctgatc catccatggg
     2641 ccgcgcgcgg gacggaggtc ctggcgatct actggtcgct cgcgccccgc gacctgtggc
     2701 agcacgcggc aagggtgtcg ggcgcgctgg gccggggcga cctggacgag gcgaagcgcg
     2761 gggtggcgat gatggtgggg cgggacgtgg agccgttgga cgagaagggc gtgacccgtg
     2821 cgacgatcga gagcgtggcc gagaacacgg tggacggcgt gaccgcgccg ctctgcttcg
     2881 cgctcctcgg cggtggtccg ctcgcgatgg cgtaccgcgc ggtcaacacg ttggactcga
     2941 tggtgggcta tcggatcgag ccctacgcgc ggatcggctg ggcgtcggcg cggctggacg
     3001 atctggccaa tctgatcccg gcgcgcctga cggcgctgct catgcccctc gccggcggtt
     3061 tcctcggcgg gtcggtccgc gggggctggt ccgcggcgtg gcgggacggg cgcaagcacg
     3121 acagccccaa ttcggggtta acagaggcgg cgatggcggg ggtgttgggg atcgagcttg
     3181 gggggccgca ctggtaccgc gggacgtact atgcatcgcc gacgatcggg accgcgaggc
     3241 gtcccgtcga gcgagccgat atcacgtggt cgtgccgtgt gatggccgtg acgacggcct
     3301 tggtgttcgt tatcggcgcc tcggcgcgac tctggatcga cccgtgaacg atttgaacgg
     3361 tttaaacgac ttgaacgatt tgaaccgcaa ttcccacggc ttggtcctcg tgtataccgg
     3421 cgacggcaag gggaagacca cggcggcgtt cggtctggcc ttgcgcgcgg cggggcgcgg
     3481 gttgcgcgtc cacgtcatcc agttcatcaa gggcgagatg gacacggggg aaatcgagat
     3541 gatcgccaag gccctgccgt cgatcacggt ggagcgggtg ggcaagggat tcacctggcg
     3601 tcgcgacgtc gtccccacgt tcgaggatca cgcggcggcg g
//
LOCUS       DBNGTT010000053         5995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_905038, whole genome shotgun sequence.
ACCESSION   DBNGTT010000053 DBNGTT010000000
VERSION     DBNGTT010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_905038"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC16BC_01205"
     CDS             complement(<1..315)
                     /locus_tag="AC16BC_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240257.1"
                     /translation="MIALRRPFALTLAGRNLRAGSRHHLPIVAAIALGVGAVVAIAAA
                     ADAARRTIAGEAKSLLAADLELRSTTPLSPTAEAAMDRLASRGARVTRVAELMAMAAT
                     PQR"
     gene            complement(312..1001)
                     /locus_tag="AC16BC_01210"
     CDS             complement(312..1001)
                     /locus_tag="AC16BC_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240258.1"
                     /translation="MIALRKVTVRLHAAGRAVTVLDDVSLDIPDPQFLAVVGPSGSGK
                     STLLAVMAGLERPTSGSVTLDGAELTALDESALARVRLNSVGFVFQSFHLIPTLTALE
                     NVAVPLELAGDARPLERARELLAAVGLETRAGHYPAQLSGGEQQRVALARACARRPPV
                     LLADEPTGNLDAESGAHVMGLLRALHAERGGTLVLVTHDVQLAASADRTIALRAGRVI
                     ADSAAVARPLP"
     gene            1073..1735
                     /locus_tag="AC16BC_01215"
     CDS             1073..1735
                     /locus_tag="AC16BC_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR3240259.1"
                     /translation="MRADEVTRAPWVISGAVCGWLLCAAFGAADATSETGTVPTIVAF
                     GDSLTAGFGVASEDAYPSRLERRLREEGYAYHVVNAGVSGDTTAGGLRRVDWVLKAKP
                     RIVILELGANDGLRGLSPERTRDNLAQIIERLRAAGATVVLAGMRVPPNYGDEYARAF
                     EAVFPELAARYQLPLIPFFLDGVATEPSLNQSDGLHPTADGYRVIVDRIWPRVRPLLT
                     RP"
     gene            1750..3270
                     /locus_tag="AC16BC_01220"
     CDS             1750..3270
                     /locus_tag="AC16BC_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR3240260.1"
                     /translation="MLTLDDLRPLTAHLIDIAHLHGAAAVLSWDQETHMPRGAAAARA
                     DALAALQGMAHERFTSPRTDDLLGRWLDLETGALFDPDVDPSARAVLREVFRDYRRAK
                     RLPTAFVALLEKTCALAHEAWLEARSARAFAPFLPHLTQIVTLKREEASYLGYTDSPY
                     DPLLDAFEPGMTAARLTPLLAEVKAGLSTLLDRVAGSSVTPIRLPAGPYDTNRQLQFG
                     TVLLSAMGYSFDRGRLDLSTHPFTTGFHPTDVRVTTRVATDDVTSSLFTCLHEGGHAL
                     YDQGLNPAYFGTPLGEAVSLGLHESQSRLWENCVGRSIEFWRHAYPLLRDAFPGPLDA
                     ISLDAFHAAINAVEPSLIRVEADELTYNFHIIMRFELEQELIAGRLLPRDLPEAWSAA
                     MRRTLGVTPSHDGEGVLQDIHWAHGAFGYFPTYTLGNLYAAQFWRQARLDIADLPTLI
                     EGGRFEPLVGWLRDRIHRWGRTYSADELVVRVTGEGLTPDHFLQYLEEKYAAIYRW"
     gene            complement(3293..4474)
                     /locus_tag="AC16BC_01225"
     CDS             complement(3293..4474)
                     /locus_tag="AC16BC_01225"
                     /inference="COORDINATES: protein motif:HMM:NF020084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR3240261.1"
                     /translation="MTTYGTGTSIHQETAIAAREACVQAASSLQRPVDLAFVFVSSHH
                     ADRFEEAAAIVAEHLAPRVVLGCTGEGIIGLDQEIELQPALSVWAAGLPGVTLSPFHA
                     TFDQGEDGIRVSGWPENMAQGSPSLFLLLADPFSTPGTEFLAFLDHHYPGAKAVGGMS
                     SGGRAPGENRLLIGETVAAEGVVGVALSGDLPITTVVSQGCRPVGGRFLITRSEGNVI
                     YELGGRPAFACLQDMYAALSPAEQELARRGLHLGLTINEAKSQFERGDFLVRGLVGAD
                     QQEGSVAVGDMVREGQTVQFHIRDRDSASEDLNTLLAAQARRIQGPAPEAALLFSCNG
                     RGRRLFGRAHHDVTLVRALLGKIPVAGFFAQGEIGPVGGSNFLHGFTASIAIFGGGSD
                     G"
     gene            4614..>5995
                     /locus_tag="AC16BC_01230"
     CDS             4614..>5995
                     /locus_tag="AC16BC_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR3240262.1"
                     /translation="MMRTACWKAVLATVLLLWARGAAAASGLDVRYSITVHFDPTDHT
                     LTGTAEVSVTNHTGMSVPEIPFVLYPNRYREADPDLDVTARERIYPRAFNPGSIAITA
                     AETAAGTPVDMAIDGGAVPPYTIARATLPRAMGPDGVVALRIQFVTFIPEKFGVFGHF
                     QGVTTLKGGWYPYVPPWRDGGWALDVPPDPAFYAVSISAPRVVTIAGAEPVDDGTGGP
                     SRRWRVETSQATGIPLAMSPFYQERVEQDASVTIRYAFLPDDRPYVRFVTATIRLGLA
                     FFRKEYGESALSEIRVAEAHLHQELVSPDPRILLVGSRAFKVFSPLKKYHESELIKSL
                     LFALWRARLPDEEAWVIEGLADASTQRYLRERYHRPPSLLRLLKPVSFIPIVDQILYS
                     NRLPLRQVYFKETTPIIPREDPLLFNNRRPDGSSIFFKLAAVIGTDALNAVLDRYTDL
                     AVHGTPRAFRA"
BASE COUNT          970 a         2096 c         1962 g          967 t
ORIGIN      
        1 ccgctgcgga gtggccgcca ttgccatgag ttccgccacg cgcgtcaccc gcgcgccgcg
       61 tgaggccagt cggtccatcg cggcctcggc ggtcggcgac aacggggtgg tggagcgaag
      121 ctccagatcc gcggccaaca ggctcttggc ttctccggca atggtccgcc gcgcggcgtc
      181 ggcggccgcc gcgatggcca cgaccgcgcc gactcccagc gcgatcgccg ccacgatggg
      241 gagatggtgt cgcgagccgg cgcgcaggtt ccgaccggcc agcgtcagcg caaagggccg
      301 acgaagcgcg atcacggcaa cggccgcgcc acggccgcgg aatccgcgat cacccgcccc
      361 gcacgcagcg cgatcgtgcg atctgcagac gccgccaatt gcacgtcgtg ggtgaccagg
      421 acgagcgtgc cgccccgctc cgcgtgcagc gcacgcaaga ggcccatgac gtgggcgccg
      481 ctctccgcgt cgaggttgcc ggtgggctcg tcggccaaca ggacgggcgg gcgtcgcgcg
      541 cacgcgcggg ccagggccac gcgttgctgc tcgccgcccg aaagctgcgc cgggtaatgg
      601 cccgcccgcg tctccagtcc caccgcggcc aacagctcgc gggcgcgctc cagcgggcgc
      661 gcgtccccgg ccaactcgag gggcaccgcc acattttcca gcgcggtcag ggtgggaatg
      721 agatggaatg actggaagac gaacccgacg ctgttcagcc gcacgcgcgc gagcgccgat
      781 tcatcgagcg cggtcaactc cgccccgtcg agggtcacgc tcccggacgt ggggcgctcc
      841 aaacccgcca tcacggccag gagcgtggac ttgccgctgc cggacggccc caccaccgcg
      901 aggaactggg ggtcgggaat gtcgagggac acgtcgtcga gcacggtcac cgcgcgcccc
      961 gcggcgtgga ggcgcacggt caccttccgc agggcgatca tcgcgcgtgg gaaaaccggt
     1021 tgcggcgcat tgcgccgcca ttatacactg aggccatgtt gcggatgcgc gagtgcgcgc
     1081 cgacgaagtg acgcgagccc cgtgggtcat atcgggcgcc gtgtgtgggt ggctgctctg
     1141 cgccgcgttc ggcgcggccg acgcgacgag tgagacggga acggttccca ccatcgtggc
     1201 cttcggcgac agcctgaccg cgggattcgg cgtcgcgagc gaggacgcgt acccgtcgcg
     1261 gctggaacgc cgactgcgcg aggaggggta cgcgtaccac gtggtcaacg ccggggtcag
     1321 cggtgacacc acggccggtg gactccggcg cgtggactgg gtcctcaaag cgaagccccg
     1381 aatcgtgatc ctggaactgg gcgccaacga cggcctgcgc ggattgtccc ccgagcgcac
     1441 ccgcgacaac ctggcacaga tcatcgaacg gctccgcgcg gccggcgcga ccgtagtgct
     1501 ggcggggatg cgggtgcccc ccaactacgg agacgagtac gcccgcgcct tcgaagcggt
     1561 ttttccagaa ctcgccgccc gctatcaact cccgttgatt ccgttcttcc tcgacggggt
     1621 ggccaccgag ccctcgctca accaatcgga cggcctgcac cccaccgcgg acgggtaccg
     1681 ggtgatcgtg gaccggatct ggccgcgcgt ccgccccctg ctcacgcgac cctgacggaa
     1741 ccgcgatcca tgctgacgct cgacgatctc cgccccctga ccgcgcactt gatcgacatc
     1801 gcgcacctgc acggtgcggc ggccgtgctc tcgtgggatc aggaaaccca catgccgcgc
     1861 ggcgcggccg cggcaagggc cgacgcgctc gccgcgctcc agggcatggc gcacgagcgc
     1921 ttcacgtcgc cgcgaacgga cgacttgctc ggtcggtggc tcgatctcga gaccggcgcg
     1981 ctgtttgacc cggacgttga cccgtcggct cgcgccgtgc tccgcgaggt gttccgcgac
     2041 taccgccgcg ccaaacggtt gcccaccgcg tttgtcgccc tgctcgaaaa aacttgcgcc
     2101 ttggcgcacg aggcgtggtt ggaggcccgc tccgcgcgcg cgttcgcccc cttcctcccc
     2161 cacctgaccc agatcgtcac gctcaaacgc gaggaagcct cgtatctggg ctacaccgac
     2221 tcgccctacg accccttgct cgatgcgttc gaaccgggga tgaccgcggc gcggctgacg
     2281 cccctgctcg ccgaggtgaa ggcggggctg tccacgctcc tagaccgcgt ggcgggatca
     2341 tccgtcaccc cgatccggct gccggccgga ccctacgaca cgaaccggca gctccagttc
     2401 ggcaccgtgc tcttgtccgc gatgggttac tcgttcgacc gcggacgcct cgacctctcg
     2461 acgcatccgt ttacgaccgg attccacccg accgacgtgc gggtcaccac gcgcgtggcc
     2521 acggacgacg tgacctcgtc gctgtttacg tgcctgcacg aaggcggcca tgcgctctac
     2581 gaccaaggcc tgaaccccgc ctatttcggc acgccgctgg gtgaagccgt gtccctggga
     2641 ctgcacgaga gccaatcgcg tttgtgggag aactgcgtgg gtcggtccat cgagttctgg
     2701 cggcacgcct acccgctgct gcgggacgcg ttccccgggc cgctcgacgc gatctcgctc
     2761 gacgcgtttc acgcggcgat caacgcggtg gagccgtcat tgatccgcgt cgaagcggac
     2821 gaactgacct acaactttca catcatcatg cggtttgaat tggaacagga gctgatcgcg
     2881 ggccgactcc tcccgcgcga tctccccgag gcgtggtccg ccgccatgcg gcggacgctg
     2941 ggggtgaccc cgtcgcacga cggcgagggc gtgctgcagg acattcactg ggcgcacggc
     3001 gcgttcggat acttccccac ctacacgttg gggaatctgt acgccgccca gttctggcga
     3061 caggcgcggc tcgacatcgc cgatctcccg acgctcatcg agggcggacg attcgaaccg
     3121 ttggtgggat ggctgcgcga ccgcatccac cgctggggga ggacctattc ggccgacgag
     3181 ttggtcgtgc gcgtgacggg cgagggcctc acgccggacc attttctgca gtatctggag
     3241 gagaaatacg cggcgatcta tcggtggtag gcgcccgtcg aagccgccga gatcatccgt
     3301 ctgacccgcc gccgaagatc gcgatgctcg cggtaaagcc gtgcaagaag ttgctcccgc
     3361 ccaccgggcc gatctccccc tgcgcaaaaa accccgccac cggaatcttg ccgagcaggg
     3421 cgcggaccaa ggtcacgtcg tggtgcgcgc gaccgaacag ccgtcgcccg cggccgttgc
     3481 aactgaacag gagcgccgcc tccggagcgg gcccctgaat ccgtcgggcc tgcgcggcaa
     3541 gcaacgtgtt gaggtcttcg ctcgcgctgt cgcggtcacg aatgtgaaac tgcacggtct
     3601 ggccttcgcg gaccatgtcg ccgaccgcca cgctgccctc ttgctggtcg gcgccgacca
     3661 gtccgcgtac gaggaaatcg cctcgctcga attgggattt ggcctcattg atcgtgaggc
     3721 cgagatgcag cccgcgacgc gcgagttcct gctccgccgg ggacagggcg gcgtacatgt
     3781 cttgcaaaca ggcaaaggcc ggacgcccgc cgagttcata gattacgttg ccttcggacc
     3841 gggtgatcag aaaccgcccg cccaccggcc ggcacccttg ggagaccacg gtggtgatcg
     3901 gcaggtcccc gctcaacgcc acgccgacca cgccctcggc ggccaccgtc tcgccgatca
     3961 gcagacggtt ttcacccggg gcgcggccgc cgctgctcat cccgcccacg gccttggctc
     4021 ccggataatg gtggtcgaga aacgccagga actccgtgcc gggcgtggag aagggatccg
     4081 cgagcagcag aaagagcgac ggggaacctt gggccatgtt ttccggccac cccgagaccc
     4141 ggataccgtc ctcgccctgg tcaaacgtcg cgtgaaaggg ggagagcgtg acgcccggaa
     4201 ggccggcggc ccagaccgac aacgcgggct ggagttcgat ctcctgatcg agcccgataa
     4261 tcccctctcc ggtgcagccg agcacgacgc gtggcgccaa atgctcggcg acgatcgcag
     4321 ccgcctcctc gaatcggtcg gcgtgatgag atgagacgaa gacaaaggca agatcgaccg
     4381 gtcgttggag actcgaggcc gcttggacgc acgcttccct cgccgcaatt gcggtctcct
     4441 ggtgaatcga ggtgccggtc ccgtacgtgg tcatcgcgcg tgtccagcac tctagcagaa
     4501 gggctttcaa ccgcgcaagc cgcgccgcca cgcgcgttcg cgctcgagcc ccctccgcgc
     4561 cccgcgctcc gcggaaaatc gaccgccccc cggtgggctg ttatactgcc cccatgatgc
     4621 ggactgcctg ctggaaggcc gtcctcgcaa ccgtcctgct gctctgggcc cggggcgccg
     4681 cggcggcgtc agggctcgac gttcggtact ccatcaccgt gcattttgat ccgaccgacc
     4741 acacgttgac ggggaccgcc gaggtatccg tgaccaacca caccgggatg agcgtccccg
     4801 agattccatt cgtgttgtat ccgaatcggt accgggaagc ggaccccgac ctggacgtta
     4861 ccgcgcggga gcgcatatac cctcgcgcct tcaatccggg atcgatcgct atcacggccg
     4921 ctgaaacggc cgccggaact ccggtggaca tggccatcga cggtggcgcg gtgccgccat
     4981 atacgatcgc tcgtgcgacg cttccgcgcg cgatgggacc ggatggcgtg gtcgcgttgc
     5041 gaatccagtt cgtgacgttt attcccgaaa agttcggcgt gttcggccat tttcaaggcg
     5101 tcacgacgct caaaggcggt tggtacccct acgtaccgcc ttggcgggat ggagggtggg
     5161 cgcttgatgt ccccccggat cccgccttct atgccgtgag catcagcgcc ccgcgcgtgg
     5221 tgacgatcgc gggcgccgag cccgtggatg acggcacggg cggcccgtcc cgccggtggc
     5281 gtgtagagac ctcccaggcc accgggatcc cgttggcaat gagccccttt tatcaagaac
     5341 gtgtcgagca ggatgcgtcg gtcacgattc ggtacgcgtt tcttccggac gaccggccgt
     5401 atgtccggtt cgtgacggcc accatccgac tgggcctcgc gttcttccgc aaggaatacg
     5461 gagaaagcgc gctgtccgag attcgcgtcg cggaggccca cctccaccag gaattggtct
     5521 ccccggaccc gcgcatcttg ctggtgggaa gccgcgcgtt caaggtgttc tcgcccctca
     5581 agaaatatca cgaatccgag ttgatcaaga gcctgttgtt cgcgctctgg cgcgcgcgcc
     5641 tgccggatga ggaggcctgg gtgatcgaag gcttggcgga cgccagcacg caacggtacc
     5701 tccgcgagcg gtatcaccgc ccccctagtc tgttgcgact gctcaaaccc gtgtcgttta
     5761 tcccgatcgt ggaccaaatc ttgtactcaa accgccttcc actccggcag gtgtacttca
     5821 aggaaactac gccgattatc cctcgggagg atcccttgct gttcaacaat cgacgccccg
     5881 acggttcgtc gatttttttt aaactcgccg ccgtcatcgg aacggatgca ctcaacgccg
     5941 tgctcgatcg gtatacggac ctggcggtcc acgggacccc gcgggcgttt cgagc
//
LOCUS       DBNGTT010000054        11747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3204810, whole genome shotgun sequence.
ACCESSION   DBNGTT010000054 DBNGTT010000000
VERSION     DBNGTT010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3204810"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16BC_01235"
     CDS             complement(<1..233)
                     /locus_tag="AC16BC_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240263.1"
                     /translation="MRGASVVVWGVLLSLTMGCTLPSTFQAIRRELADLPAGPAVPDL
                     PGLSRYKGVIHVHTRLSHDSRGEESEIARAAKSA"
     gene            complement(265..1026)
                     /locus_tag="AC16BC_01240"
     CDS             complement(265..1026)
                     /locus_tag="AC16BC_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR3240264.1"
                     /translation="MAGVRPHRQRADVALVERGLAPSRARAQAMILEGLVRSGDRPVA
                     KAGDLIAEDAPLSVTGADHPYVSRGGVKLAAALDAFAVDPRGLVCLDVGASTGGFTDC
                     LLQRGAVRVWAVDVGYGQLAWSLRTDPRVRLLERANIRTLAREAITEPIDLAVIDVSF
                     ISLRLVRPRVAQLVRPGGGIIALIKPQFEVGREHVGSGGIVRDSAQRARAVREVAIAA
                     EALGFRCAGSIPSPIEGRDGNQEYLIALAAADRVA"
     gene            1138..2946
                     /gene="ftsH"
                     /locus_tag="AC16BC_01245"
     CDS             1138..2946
                     /gene="ftsH"
                     /locus_tag="AC16BC_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3240265.1"
                     /translation="MDKKQVRFSVWYLVIALWVIALIQLFVAPAFGPSELSYSEFKAA
                     VAAGKVDDVSISPTVIHGRLKPEQAGEAVRPFNAIRVEDPDLLKDLQAQGVKVTGVIE
                     STFLRDLLSWVIPILLFAGVWVFLLRRMGQGQGGFMTVGKSKAKIYMEQDIKARFTDV
                     AGVDEAQEELREVIEFLKTPGKFTRLGGKIPKGILLVGPPGTGKTLLARAVAGEAGVP
                     FFSISGSEFVEMFVGVGAARVRDLFLQAKQKAPCIIFIDELDALGKVRGMGPMAHEER
                     EQTLNQLLVEMDGFDPRVGVILMAATNRPEILDPALLRAGRFDRHVVVDRPDRLGRIA
                     ILKVHAKNVPLAPDADLEAVAGMTPGFVGADLANLINEAALLAVRRGKDLVGSSELQE
                     AVERVIAGLEKRNRVLNKFERERVAHHEIGHALIALSIPGMHPVQKISIIPRGVAALG
                     YTLQLPTEDRFLMTKSELEDKIAMLLGGRVAEEIIYQEVSTGAQDDLMKATDIAKSMV
                     KTYGMSGKLGGVTFDGARHPTFLPTGQATGPADYSEETAREIDCEVRDILDTQFTRVR
                     SLLQARQDALRRAAVALLKTETMTGDELQALVGASG"
     gene            2996..4807
                     /locus_tag="AC16BC_01250"
     CDS             2996..4807
                     /locus_tag="AC16BC_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR3240266.1"
                     /translation="MTDAIRYRITPSDPAAHLFEVRCAISHPASDGQRFSLPTWIPGS
                     YLIRDFARHVVRVSAESGGRPVAVTKLDSHTWACEPVSGPLTVISEVYAWDLSVRAAH
                     LDAMHGYFNGTSVFFQIHGQEHRPCAVEIAPPSGETGRTWRVGTALRRDGAPPHGFGR
                     YAAGDYDELMDHPVEMGDFTLATFSACGVPHDVILTGRHRADVARLCRDLTAVCEYHI
                     RFFGEPAPFDRYVFLITAVGEGYGGLEHRASSSLSCSREDLPKPGDRAVTEKYRTFLA
                     LASHEYFHAWNVKRIKPQAFMPYDLAREAYTRLLWAFEGITSYYDALALVRTGLIPPA
                     SYLDLLGQTVTRLLRGPGRLKQTVEDSSFDAWIKFYKPDENTPNAVVSYYTKGALAAL
                     ALDLTIRRETRNAKSLDDVMRALWDRYGKTGAGVPEDGIERIAGEVSGTDLREFFDRT
                     LRTVEDLPLADLLADVGVALNLRAAESDQDKGGKAGATPQTTKAVLGVRLAEGGSDAK
                     LATVYDGSAAHAAGLAAGDVIVAVDGLRAGRGNIEPLIGAYQPGAVVPIHAFRRDELM
                     TFDVSLKPAPMDTCFLTLRDEIDDPTRARRATWLGRG"
     gene            complement(5111..7315)
                     /locus_tag="AC16BC_01255"
     CDS             complement(5111..7315)
                     /locus_tag="AC16BC_01255"
                     /inference="COORDINATES: protein motif:HMM:NF027967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3240267.1"
                     /translation="MALAAVLFLLIGTAPAFGADAILSWDANTEQDLAGYTVYYGTTS
                     GVYSVIVPVGLVTTYTVTGLGPGTYYFAVTAENTSGLSSSYSSQVSKTIADTTPPTIS
                     AVVASGITASGATVTWTTDEASTSAAEYGPTTSYGASSALDSTRVTAHSRTLSGLTPS
                     TTYHFRVLSVDATGNVGVSADYTLTTAAASDTTPPAISNTTSGSITSTGAVITWTTDE
                     AATTQLDYGTTTAYGASTTLNATLVTAHSAALTGLQAATLYHYRVRSSDAAGNAAVSS
                     DATFATAAAPDTAAPVISGTTSSTISATSAVITWSTNEASTSAVDYGLSTAYGLSSSN
                     ATLVTSHSLTLTGLTASTAYHYRVRSSDAAANAASSGDFVFTTSAAPDTTAPTLTSIA
                     AGSLTSAGATITWTTNEAASTQVDYGLTTAYGASTTLNATLVTAHSAALTGLQAATLY
                     HYRVRSSDAAGNAAVSSDRTFTTAQAPDTTAPTISNLSSTDATPTTAVITWTTDEAAT
                     SQVEFGSTTAYGRQTTANATLATSHRETLSGLTPGTTYHVRVLSADAAGNRSTSGDLA
                     VAIPAAPDTTPPQDISGFTSSGGPGQVTLTWTNPPDADFMGVQIRFRIDRYPSGPTDG
                     ELLGDFTGQPNEGGQAVHEGLLNGVTYYYAAASYDQSGNRQSTVYTSATPLPQGTSVD
                     QPTTAGGCGMILPSGGDPPGPWQAADLLILAGVALYLMARRRPGPGYRPASV"
     gene            8259..8735
                     /locus_tag="AC16BC_01260"
     CDS             8259..8735
                     /locus_tag="AC16BC_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HIR3240268.1"
                     /translation="MTVHSPPPTTDALIAHCTCMVVSFRHWTGRDLIGGTTSGTDVVE
                     RLFDAPFVLLSHSAEADPILTFGNRMALTLWETTWERFTRMPSRLTAEPVARDERARA
                     LADVARRGYVEDYRGVRVSGTGRRFLIERAVVWSLIDSVGRVTGQAAMFDTWTELE"
     gene            8748..9059
                     /locus_tag="AC16BC_01265"
     CDS             8748..9059
                     /locus_tag="AC16BC_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR3240269.1"
                     /translation="MARLTVKVIPKSSRNRVVGWLGDALKVTVTAPPEGGRANAAVAE
                     LLAKSLDVAASSVLCVAGHGSSRKLFDICGLNAEQVRERLRTFMSPEDARRDDPPAGR
                     P"
     gene            9455..10564
                     /locus_tag="AC16BC_01270"
     CDS             9455..10564
                     /locus_tag="AC16BC_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3240270.1"
                     /translation="MVLVVGVLIWLGYGTLVGQAAAPPRPAFNHDRTHFPLTGEHKKV
                     ECEACHTGGRYENLPTQCAICHNGQRATGKPVGHVMTAAQCDTCHSTTKFAGAAFQHD
                     PATTAGKCDTCHNGRTALGKPSGHIATTAQCDTCHSTTTFAGVGYQHDASAVGKCDTC
                     HNGRTAVGKPASHIATTAQCDTCHTTTSFAGATFRHDGTTAGKCDTCHNGRTALGKPT
                     THIATTAQCDICHTTTSFAGATYKHDSSATGMCDKCHKGTIATGKPSTHGAISLQCDQ
                     CHRTTSWSPATYPLHANPSLTGGHSGLVCTVCHPQTFTSPYYRDGVQYGFCSNCHRRD
                     YTYPDHHSKWGTSFNTSLQINANCLSCHSYSTFAH"
     gene            10564..>11747
                     /locus_tag="AC16BC_01275"
     CDS             10564..>11747
                     /locus_tag="AC16BC_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3240271.1"
                     /translation="MRCGRWYWAVGLVSLALFVHGCGGTQPRTVASPRFSNPEIEASY
                     KNGEEALLGGDYVRARRVLMRIISRYPGENDLAQVQWLIAKTYDMEGKREEAISEYKR
                     FLANYPDHPDQFTVEYADDRIRQLERAARPKPAKAVRVVGALSTDYEYQKDLSPDPLT
                     TLNRMTTRLDAQIRNLDAGRGKVVVSALRSFDFEDRDGDRSRLQKLYGDWRNAAETFS
                     ARFGRQPSTPGALATRYDGAELHYRFIPTVAFDAAAGFPVEFSQRGSLSTDVHFYEAG
                     LSMADAGGATGRVYAVQQFTEDVIDREAVGGNLQAQWGRVGLSGNLDYDTSFGAFNDR
                     FLSVEYALRESLHLTVARDVRNDPYLQMRTALQDDAATTATPLITSVSDLLAQPGQSE
                     ETV"
BASE COUNT         2135 a         3669 c         3814 g         2129 t
ORIGIN      
        1 gctgatttcg ccgcgcgagc gatctccgat tcttctccac gactgtcgtg cgacaaccgc
       61 gtgtggacat ggatcacgcc tttatatcgt gacaggccgg gaaggtccgg tactgcgggt
      121 cccgcgggga ggtcggcgag ttctcgacgg atcgcctgga acgtcgacgg gagggtacat
      181 cccatcgtga ggctgagaag aacgccccac acgaccacgg atgcaccgcg cataccgaaa
      241 tagtaatgga acgttctgaa gcgatcaagc gacgcgatcc gccgccgcaa gcgcgatcaa
      301 gtattcctga ttgccgtcac gcccctcgat cggcgacggg atgctccccg cgcatcgaaa
      361 ccccagcgct tcggccgcga tcgccacctc ccgcaccgcg cgggcgcgct gcgccgagtc
      421 gcgcacgatg cctcccgaac ccacgtgctc gcgtcccacc tcgaattgcg gcttgatcaa
      481 ggcgatgatc ccccccccgg gacggacgag ttgcgcgacg cgcggccgca ccagacgtaa
      541 tgaaatgaac gagacgtcga taaccgctag atcaatcggc tccgtgatcg cttcacgagc
      601 cagcgtccgg atattcgcgc gctccagcaa tcgaacgcgc ggatcggtcc gcaatgacca
      661 cgccagttgt ccgtacccga catccacggc ccatacccgg accgctcccc gctggaggag
      721 acagtccgtg aagcccccgg tcgaggcgcc gacgtccagg cacaccaacc cgcgcgggtc
      781 caccgcgaac gcatcgagcg cggccgcgag tttgaccccg ccccggctca cgtaggggtg
      841 gtccgcgccc gtgacgctga gcggcgcatc ctcggcgatc aggtccccgg ctttggcgac
      901 cggacgatcg ccggagcgca caaggccctc caggatcatg gcctgggcgc gggcgcgact
      961 gggtgccagg ccgcgctcga ccaacgcgac atcggcccgt tgcctgtgcg gtcgcactcc
     1021 agccataatc ggacggtatc agagcccacg cgaattgtgc aaccagcctc ttgcgctttg
     1081 cggggtgctc cgtgtattac acgggaaggg cggatgcggc cggaaggagc ggggctcatg
     1141 gataaaaaac aggttcgctt ctcggtgtgg tacctcgtga tcgcgttatg ggtcatcgcg
     1201 ctgatccagc tcttcgtggc tccggcgttc ggaccgtcgg aactctccta cagcgaattt
     1261 aaagccgcgg tcgcggccgg caaagtcgac gacgtgtcca tctcccccac ggtcattcac
     1321 ggacgcctca aacccgaaca ggccggcgag gcggttcggc ccttcaacgc gatccgggtg
     1381 gaggatcccg acttgctcaa ggatctccag gcccaaggcg tcaaggtgac cggcgtgatc
     1441 gagagcacct tcttgcgcga cctgctgtcg tgggtcatcc cgattctctt gttcgccggc
     1501 gtgtgggtgt tcttgctccg tcgcatgggg cagggccaag gcggtttcat gaccgtgggc
     1561 aagagcaaag ccaagatcta tatggagcag gacatcaagg cgcggttcac cgacgtggcc
     1621 ggcgtggatg aagcgcagga ggaacttcgc gaggtcatcg agtttctcaa gacgccgggc
     1681 aagttcacgc gattgggggg caagatcccg aaaggcatcc tgttggtcgg cccgccggga
     1741 accgggaaaa ccctgctggc acgcgcagtc gcgggcgaag ccggcgtgcc gtttttctcg
     1801 atcagcgggt cggagttcgt tgaaatgttc gtgggcgtgg gggccgctcg ggtgcgcgac
     1861 ctgttcctcc aggccaaaca aaaggcgccg tgcatcatct ttatcgacga actggacgcg
     1921 ctgggcaagg tgcggggtat gggcccgatg gcgcatgaag aacgcgagca aacgctcaac
     1981 caacttctgg tggaaatgga cggttttgac ccgcgcgtcg gggtaatctt gatggccgcg
     2041 acaaaccgcc cggagattct tgaccccgct ttactgcgcg cgggccggtt cgaccggcac
     2101 gtggtcgtgg accggccgga tcgcctgggc cggatcgcca ttctcaaagt gcacgcaaaa
     2161 aacgtcccgc tcgcgccgga tgccgacctc gaagccgtgg ccggaatgac gccggggttt
     2221 gtgggcgcgg acctcgccaa tctgatcaac gaagccgcgc tgctggccgt gcgtcgagga
     2281 aaagatctgg tcggctcgtc ggagttgcag gaggcggtgg aacgggtgat cgcggggctt
     2341 gaaaaacgga accgtgtctt gaacaagttt gagcgtgagc gcgtagcgca ccatgaaatc
     2401 ggccacgcac tgatcgccct gtccatcccc ggcatgcacc cggtgcaaaa aatctcgatt
     2461 atcccgcgcg gcgtggccgc cctcgggtac acgctgcagt tgccgaccga ggaccggttt
     2521 ttgatgacca agtccgagct cgaagacaaa atcgccatgc tcttgggagg gcgggtggcc
     2581 gaagagatca tttatcagga ggtatcgacc ggggcccagg acgatctgat gaaggcaacg
     2641 gacatcgcca agagcatggt caagacctac ggtatgagcg ggaagttggg cggggtgaca
     2701 ttcgacggcg cccgccaccc cacgttcctt ccgacggggc aggcgaccgg ccccgcggac
     2761 tacagcgaag agaccgcgcg ggagatcgac tgcgaagtcc gcgatatcct ggatacccag
     2821 tttacgcgtg tgcgcagtct gctgcaggcg cgccaggacg cgttacggag ggccgcggtt
     2881 gcgctcttga aaaccgagac catgacgggc gacgagctgc aggcgctcgt cggcgcgtcc
     2941 ggctaatttt gaggcccgcc accgcgatgt cgttacgctc tgatacaatc gcgcggtgac
     3001 cgacgccatc cggtaccgca ttacgccgag cgatcccgcc gcccacttgt tcgaggtgcg
     3061 gtgtgcgatc tcgcatcccg cctccgatgg acaacgattc tccctcccga cgtggattcc
     3121 cggcagttat ctgatccgag attttgcccg ccacgtcgta cgggtgtcgg ctgagtccgg
     3181 cggacgcccg gtcgccgtga ccaagcttga cagccacacg tgggcgtgcg agccggtgtc
     3241 cgggccgctc acggtgatca gcgaggtgta cgcgtgggac ctctcggtcc gcgcggccca
     3301 tctcgacgcg atgcacgggt atttcaacgg caccagcgtg ttcttccaaa tccacggcca
     3361 ggagcaccgg ccgtgcgccg tggaaatcgc gcccccgtcc ggagaaaccg gccgaacgtg
     3421 gcgcgtcggg accgcgctgc gccgcgacgg agccccgccc cacgggttcg gtcgatacgc
     3481 ggccggggac tatgacgaat tgatggatca tccggtcgag atgggcgact tcacgctcgc
     3541 gacgttcagc gcctgcggcg tgccccacga cgtcattctg accggacggc accgggcgga
     3601 tgtggcgcga ctctgccgcg acctcacggc cgtctgcgaa taccacatcc gtttcttcgg
     3661 tgagcccgcg ccgtttgacc gctatgtgtt tttgatcacc gcggtgggcg aaggatacgg
     3721 cggcctggag caccgggcat cgagcagtct gtcgtgttcg cgcgaggacc tccccaaacc
     3781 cggtgatcgc gcggtcaccg agaaataccg gacgttcctg gcgctggcca gccacgagta
     3841 tttccatgcg tggaacgtca aacgcatcaa gccccaagcc ttcatgccgt atgacttggc
     3901 gcgcgaagcc tacacccggt tgttgtgggc gttcgagggc atcacctcct attatgatgc
     3961 gctggccctt gttcggaccg gactgatccc tccagcaagt tatctggacc tcttgggcca
     4021 aaccgtcacg cgcctgcttc gcggtcccgg gagactgaaa caaaccgtcg aggactcgag
     4081 ttttgacgcg tggatcaagt tctacaagcc ggacgagaac accccgaacg ccgtggtgag
     4141 ttactacacc aagggggcgc tcgcggcgct ggcgctggat ctcacgattc gacgcgaaac
     4201 gcggaacgcc aaatcgctcg atgatgtcat gcgggcattg tgggaccggt acgggaagac
     4261 cggagcgggc gtccccgaag atggcatcga gcggatcgcg ggagaagtgt cgggaacgga
     4321 tcttcgcgag ttttttgacc ggacgcttcg cacggtcgag gaccttcccc tggcggacct
     4381 attggccgac gtcggcgtcg cgctcaacct gcgcgcggcc gagtccgacc aggacaaggg
     4441 cgggaaggcc ggggccaccc cacaaacgac caaggccgtc ctcggcgtcc gccttgccga
     4501 aggtggatcg gatgcgaaac tggcaacggt ctacgatggg agcgcggcac acgccgcggg
     4561 gcttgccgcc ggagacgtga tcgtggcggt ggatggattg cgcgctggac gcggcaacat
     4621 cgagccgctg attggcgcct accagcccgg cgccgtggtg cccatccacg cgttccgacg
     4681 cgacgagttg atgacgttcg acgtctcgct gaaacccgcg ccgatggaca cctgtttcct
     4741 cacattgagg gacgagatcg acgacccgac tcgggctcgc cgcgcaacgt ggttgggccg
     4801 cgggtgattg gcgaggcagc cgcgggccgt ggcacctccc cgctcccttg acagtttgct
     4861 catacctgcc accttcacgt caggtcgggt cgcggtgata ggagctgggg atgaagcggt
     4921 gggcggtgtt ctgggcgtgc ctgtcggtcg taccgatttc gttcgacagt ccccaggata
     4981 ctgccccgat cgacatccat gctcagcggc ccgtcgctcc cgccggatcg tcggcgcgag
     5041 ccggctcgcc tgacgacctc ccggacgcgg ctgacgccag cgtcggtccg aacgactcga
     5101 tccgctcccg tcagaccgac gctggtcggt aaccgggacc aggccgtcgc cgcgccatta
     5161 aatacagagc caccccagcc aaaataagca ggtctgcggc ctgccacggt cccggaggat
     5221 caccgccgct cgggagaatc atgccgcagc ctcccgccgt tgtcggctgg tcgaccgagg
     5281 tgccctgcgg caatggcgtc gcggacgtgt acaccgtgct ctgtcggttg ccggactgat
     5341 cgtacgatgc ggcggcgtag taatacgtca cgccgttgag caagccctcg tggaccgctt
     5401 gaccgccttc gttgggttgg ccggtaaaat ctcccaacaa ctctccgtcc gtaggtccgg
     5461 agggataccg gtcgatccgg aagcggatct gcacgcccat aaagtccgcg tcaggggggt
     5521 tggtccaggt caaggtcacc tggcccgggc cacccgagga agtgaacccc gagatatcct
     5581 ggggcggggt ggtgtccgga gcagcgggaa tggccacggc gaggtccccc gacgtggatc
     5641 tgtttccggc ggcatccgcg ctgagcacgc ggacgtggta ggtggtaccc ggcgtgaggc
     5701 cagacagcgt ctcgcgatgg ctggtggcca acgtggcgtt cgccgtagtt tgtcgtccgt
     5761 acgcagtggt ggagccgaat tccacctgcg atgtggcggc ttcatcggtg gtccacgtga
     5821 tgaccgcggt ggtcggagtc gcgtcggtcg acgacagatt cgagatcgtg ggcgccgtgg
     5881 tatccggagc ctgtgcggtc gtgaaggtcc tgtcactgga aacggcggca ttgcccgcgg
     5941 cgtcgctgct gcgcacccga tagtgataga gagtggcggc ctggaggccg gtgagtgcgg
     6001 ccgaatgcgc ggtcacgagc gtcgcgttga gcgtcgtcga ggcgccgtac gccgtggtca
     6061 gaccgtagtc gacctgcgtt gaagcggcct cgttggtggt ccaggtaatc gtggcgcccg
     6121 cgctggtgag ggacccggcc gcgatgctcg tgagcgtggg ggccgtggtg tccggcgccg
     6181 cggaggtcgt gaacacgaag tccccggatg acgccgcgtt ggccgcggca tcgctgctgc
     6241 gcacgcgata gtggtacgcc gtggatgcgg tcaagccggt cagcgtcagg ctgtggcttg
     6301 tgacaagcgt cgcgttggac gatgacaacc cgtaggccgt actgagtcca tagtcgaccg
     6361 cggaggtgga ggcctcgttg gtactccacg tgataaccgc gctcgttgcg ctgatggtgc
     6421 tggaggtggt gcctgagatg accggcgcgg cggtatccgg cgcggccgcc gtggcgaagg
     6481 tcgcgtcact ggagacggcg gcattccccg cggcatcgct gctgcgcacc cgatagtgat
     6541 agagagtggc ggcctggagg ccggtgagcg cggccgaatg cgcggtcacg agcgtcgcgt
     6601 tgagcgtcgt cgaggcgccg tatgccgtgg tggtgccgta atcgagctgc gtggtcgcgg
     6661 cctcatccgt tgtccacgta ataacagccc ccgtgctggt gatggatccc gaggtcgtgt
     6721 ttgaaatcgc ggggggcgtc gtgtcggaag ccgcggcggt ggtgagggta tagtccgccg
     6781 ataccccgac gttgccggtc gcgtcgacgc tcaacacccg gaaatggtac gtggtggatg
     6841 gcgtcaatcc actgagcgtc cggctgtgtg cggtgacccg ggttgaatca agtgccgaag
     6901 acgcgccgta actcgtggtt ggaccgtact cagccgcgga agtggaggct tcgtcagtag
     6961 tccatgtcac cgtggccccg gaggcggtga taccgctggc cacgaccgcg gaaatcgtgg
     7021 ggggggtagt atcggcgatg gtcttactca cttggcttga gtagctgctg ctgaggccag
     7081 aggtgttttc tgcagtcact gcgaaatagt aggtgccggg gcccaacccg gtcacggtgt
     7141 aggtggtcac aagtcccacc ggaacaatga cggagtagac gcccgaagtg gtcccgtagt
     7201 agacggtgta tcctgccaga tcctgctcgg tgttggcgtc ccatgacagt atggcgtctg
     7261 ctccaaacgc cggtgccgtg ccgatgagca ggaagagaac ggctgcgagg gccaagcttg
     7321 cggagccacc aagcaacggt ttcttcggct cgcgctctga cgttcgatga ctgcgatgca
     7381 tgacaatgtc ctgcctttct gttgcggtgc ctgcgacgac ccctcgcgcc cggtccgtgt
     7441 ggcgcggctt gccaaccgag cgtcgtcaag caaacgtggt gccagcatcg cgcaggcacc
     7501 gaaaagcgcc gtcgcaaccc ggcaatcgcg tcacccgcgc gttccgaagg ccagtccccg
     7561 ggacgcgttc tccgcggatt gtggcgcgaa atccgtgatt cgaattaccg ttcggtcggg
     7621 tcagtggcgg ctatcactgc aaccgggacg cgtcgcacga cgtgggactt ctgccgaaat
     7681 ctcaccgaag tgcgacaggc tctgccagct ttccagcacg gcccaagtgt cagtgagggc
     7741 gccgccgttc cgttgcgaaa agacactgct ccatctctcg attttttctt cgcgacgtgc
     7801 tatggggtat gcgatgcccg cattaaacat tgcgcgcctg acgggcgcct ggcccgacac
     7861 aagaactcaa cgcgcataca acgttcagac cacagtgcct tgagggattg tgcctctggc
     7921 tgtctgccaa gcggatcgcg atgcgttggc gagggcgggc ggcacggaaa tcgagtcagc
     7981 ggcgatcggc tttttcgggt gctttgcccc atgattctat ttgacccatg attccagcgg
     8041 acgcgacctc gggacaaatc atgcaagccg tgttcgacca acgcatctgg tgcgttgtcg
     8101 cctctacgga agatgaatgt cgcgggtggg ccacccgtca acggcatcgc cggagcgcgg
     8161 gccatgcgtt ctcgttggtg tagcccgtgt gctgaatcgc gagcgacgca gactattcgc
     8221 cggccgtcgc ctacgcgaac ggcgcggttc gagatgtcgt gaccgtccat tcgccccctc
     8281 cgactactga cgcgctcatc gctcattgca cgtgcatggt tgtgagtttc cgtcattgga
     8341 ccggtcggga cctgatcgga ggcaccacat ccggcaccga tgtcgtcgaa cggttgttcg
     8401 acgccccgtt tgtgcttctc tcacacagcg ccgaagcgga cccgattctc accttcggga
     8461 accgaatggc cctgaccctc tgggaaacga cgtgggaacg gtttacccgc atgccgtctc
     8521 gtctgactgc cgaacccgtg gcgcgtgatg agcgggctcg agccttggcg gacgtggccc
     8581 gtcgagggta cgtggaggat taccgcggcg ttcgggtctc cggaaccggt cgtcgattct
     8641 tgatcgagcg cgccgtcgtc tggtcattga tagactctgt cgggcgcgtc acaggacaag
     8701 ccgccatgtt cgacacttgg accgaacttg aataacggag cgatcgagtg gcccgtttga
     8761 cggttaaggt catcccaaag tcttcgcgga accgcgtggt tggctggctt ggagatgcct
     8821 taaaggtcac cgtgaccgca ccgcccgaag gtgggcgggc caatgctgcg gtcgcggaac
     8881 tgctggccaa gtctcttgat gtcgcggcat caagcgtgct gtgcgtcgcg ggacacgggt
     8941 cgtcgagaaa actctttgac atctgcgggc tgaatgcaga gcaggtgcgt gagcggcttc
     9001 gcacgttcat gagtcctgaa gacgcccgtc gtgacgaccc ccccgccggg cggccttaac
     9061 cgtttcttaa tagtcgaccg gctacactca ccgcgctctg tggcggagaa gctcggatcg
     9121 cgcacgggac acccggacgc cgagccccct ccagactggc tcgactgcct taagagggac
     9181 tacgcgctgg ccgagaattt gacgagcact tgtacgggct gacggaaatt cggggtaacg
     9241 aaggaatcca acttccgtat ttcgagtgat taaaatgtgt aattcccgcg gtaaatgaac
     9301 cccgggagcg ggctccgggc ggatcggcca atggtatgga aattgctgga tttcacccgt
     9361 tgctgaaacc gcgaagtggg cggttaacca ggagcacaga gccgatatgt catccataga
     9421 acgagatcgc gtgcgttgcg caagccgacc tctcgtggta ctagtcgtcg gagtcctgat
     9481 ctggctcggt tacgggacat tggttgggca ggccgccgcg ccgccccgtc ccgcgttcaa
     9541 ccatgaccgc acgcacttcc ccttgacggg ggaacataag aaagtcgagt gcgaagcgtg
     9601 tcataccggc gggcgatatg aaaatctccc gacccaatgc gcgatctgtc acaatggcca
     9661 gcgcgcgacc ggcaagcccg tcggccacgt gatgaccgcg gcgcagtgcg atacctgtca
     9721 ttcgacgacg aagttcgccg gagcggcgtt ccaacacgac ccggcaacca ccgcggggaa
     9781 atgcgatacg tgccacaacg gccgcaccgc cctcggcaag ccgtccggac acatcgccac
     9841 gacggcgcaa tgcgacacct gccacagcac gacgaccttc gccggcgtcg ggtaccaaca
     9901 tgacgcgagc gccgtgggca agtgcgatac ctgccacaat ggtcgcaccg ccgtcggcaa
     9961 acccgcaagc catatcgcga cgaccgccca gtgcgacacc tgtcacacca ccaccagctt
    10021 cgcgggcgcc accttccgcc acgacggcac cactgccggc aagtgcgaca cctgccacaa
    10081 cggccgcacc gccctcggca aacccacgac ccatatcgcg accaccgccc agtgcgacat
    10141 ctgtcacacc accaccagct tcgcgggcgc gacgtacaaa catgattcct cggcgaccgg
    10201 tatgtgcgac aagtgtcaca aggggaccat cgcaacgggg aaaccttcta cgcatggcgc
    10261 gatttcgttg cagtgtgatc agtgccatcg cacgacaagt tggtccccgg ccacgtatcc
    10321 gctccacgcg aacccgtctc tgaccggtgg tcattcggga ttagtgtgca ccgtctgcca
    10381 tccgcagacg ttcacatccc cgtactatcg cgatggcgtc cagtacgggt tctgttcaaa
    10441 ttgtcaccgt cgggactaca cgtacccgga tcaccacagc aaatggggca ccagctttaa
    10501 tacctcgctg caaatcaacg ccaattgcct cagttgtcat tcctattcga ccttcgctca
    10561 ctgatgcggt gtggtcgctg gtactgggcg gttgggctgg tgagcctggc gctgtttgtc
    10621 cacggttgcg gcggaacgca accacgtacc gtggcgtccc cgcggttttc gaaccccgag
    10681 atcgaagcgt cgtacaagaa cggggaagaa gcgttgttgg gtggcgacta tgtccgggcg
    10741 cgccgggtat tgatgcggat catttcgcgg tatcccgggg agaatgattt ggcgcaggtc
    10801 cagtggttga tcgccaagac ttacgacatg gaggggaaac gtgaggaagc tatttccgag
    10861 tataagcgtt tcttggctaa ttaccccgac caccccgatc agttcacggt cgagtatgcc
    10921 gacgaccgta ttcgtcaact ggaacgagcc gctcggccca agccggcaaa agcggtccgc
    10981 gtggtgggcg ccctctcgac cgactatgaa taccagaagg acctgagccc cgacccgctc
    11041 accactctga accgcatgac cacgcgcttg gacgcccaga tccgcaacct cgacgcggga
    11101 cgcggaaagg ttgtcgtcag cgcgctgcgc tcgtttgatt ttgaagaccg cgacggtgat
    11161 cgttcgcgcc ttcaaaagct ctatggcgac tggcgcaacg ccgccgaaac attctccgcg
    11221 cgtttcgggc gccaaccgtc gacgccggga gccttggcaa ctcggtacga tggggccgag
    11281 ctccattacc gatttattcc gacggtcgcg ttcgacgcgg ccgcaggctt tccagtcgaa
    11341 ttttcccagc gcggatcgct ctcaactgat gtccatttct acgaagccgg tttgagcatg
    11401 gcggacgccg gcggcgccac cggacgggtg tatgccgtgc aacagttcac cgaagacgtg
    11461 atcgaccgag aggccgtggg cggcaatctc caggcgcagt gggggcgggt tgggctgagc
    11521 ggcaacctgg actacgatac gagcttcggc gcgttcaacg accggttcct ttctgtcgag
    11581 tacgcgctca gagagtcgct ccacctcaca gtggcccgcg acgtgcgcaa tgatccctac
    11641 ctacaaatgc ggacggcact gcaggatgat gcggccacca ccgcgacgcc actcatcaca
    11701 tccgtctccg acttgttggc tcagcccgga caaagcgaag agacggt
//
LOCUS       DBNGTT010000055         3294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_905108, whole genome shotgun sequence.
ACCESSION   DBNGTT010000055 DBNGTT010000000
VERSION     DBNGTT010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_905108"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2563)
                     /locus_tag="AC16BC_01280"
     CDS             complement(<1..2563)
                     /locus_tag="AC16BC_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3240272.1"
                     /translation="MFLPELSIRRPVLATVMSLMLILIGIISYTRLAVREYPNIDAPI
                     VSVRTVYTGASADVVESQISQPLEDSLAGIEGIRTIKSISREEVSQITVEFTLERDID
                     AAANDVRDRVARVRGLLPEEADEPVIAKIEADAFPVIWVAFSSDRHSPLEITDYADRV
                     VKDRLQTLPGVASVIIGGERRYAMRLWLDRERLAAYGLTPQDVEAALRSQNVQVPSGR
                     IESRQREFTVLTETDLRAPAEFNRLIIREVNGYPVRLADVGHAELGAADDRNAVRVNG
                     NPAVGVGVVKQSNANTLAVAKAVKAALPGLTAGLPEGMKLQIGFDSSIFIDESIKSVF
                     ETLAEALALVVLVIFYFLRSGRATLIPFVTIPVSLVGAFIFVYALGFSVNVLTLLALV
                     LAIGLVVDDAIVMLENIYRRIERGMAPWQAALEGSREIAFAVLAMTITVAAVFVPVAF
                     MTGTTGRLFREFALTVSAAVLVSGFVALTLTPMMCAKLLRHERQHGRLYGMIERGIER
                     LNTGYRGLLTRSLTRRWLIVGLGVAAAGSIVYLFTALKAELSPLEDRGTIIGVMLAPE
                     GATMAYTDGYARRVEALYSQVPEIRTYFMVVAPGLERPNPVTSAFSFVSLKPWAERER
                     KQQAIVGELMPKMFGLPGVLAFAINPPSLGQSFRNPPLMFVVQGNTYPELQTMVDKLM
                     AKVREFPGLVNADTDLKLNKPQFSVHLDREKTADVGVEVETVGRTLETLLGGRQVTRF
                     KREGEQYDVIVKVADKDRTTPDDLTAIYVRGTNGQLVQLANLVQVKETVAPKELNHFN
                     RLRAATISANLAPGVALGDAIAFMDKAAKEVLPPGTQTSLDGQSREFKEAGASMYI"
     gene            complement(2565..3182)
                     /locus_tag="AC16BC_01285"
     CDS             complement(2565..3182)
                     /locus_tag="AC16BC_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3240273.1"
                     /translation="MPTIRAPFGGTLGLRQVSPGDYLQPGQAVVNLEDVSVVKVDFRV
                     PEAGVGKVGVGQSVSVRVDAFPDRAFTGTIVAVDPRLDETTRTAMVRARVPNPRGELR
                     AGMFARVAVIIGERANAILIPEQAVVPMGNDAFVFRVVDGKAAMVKVVIGRRAAGEAE
                     IMSGLNADDLVVTGGQMKIRDGAPVAVINQGAPSGPGAGASPPKG"
BASE COUNT          580 a         1144 c         1021 g          549 t
ORIGIN      
        1 cgatgtacat gctggcgccc gcctccttga attctcgcga ctggccgtcg agcgaggttt
       61 gcgtgccggg cggcaagact tctttggccg ccttgtccat gaacgcgatc gcgtcgccca
      121 gagccacacc cggcgccaga ttcgcggaaa tcgtcgcggc ccgcagccgg ttgaagtgat
      181 tgagctcctt gggcgccacg gtctccttga cttgcaccag attggcgagc tgcaccaact
      241 ggccgttggt cccccgcacg tagatcgccg ttaaatcatc cggcgtggtc cggtccttgt
      301 ccgcgacttt cacgatcacg tcgtactgtt cgccctcgcg cttgaaccgc gtgacctgcc
      361 gcccgccgag cagcgtctcc aacgtccggc ccacggtctc aacctccacg cccacgtccg
      421 cggtcttctc ccggtccagg tgcacggaaa actgcggctt attcagtttg agatccgtgt
      481 ccgcgttgac caagcccggg aactcccgca ccttggccat caacttgtcg accatggtct
      541 gcagctcggg atacgtgttg ccctggacca cgaacatgag cggcgggttg cgaaaactct
      601 gtcccaatga cggcgggttg atggcgaacg cgagcacgcc gggcagcccg aacatcttgg
      661 gcatcaactc cccgacgatc gcttgttgct tacgttcgcg ctccgcccac ggcttgaggc
      721 tcacaaacga gaaggcggag gtcacgggat tgggccgctc caggccgggc gccaccacca
      781 taaagtaggt tcggatctct ggcacctggc tgtacaaggc ttccacgcga cgcgcgtagc
      841 cgtcggtgta cgccatggtc gccccttcgg gcgcaagcat cacgccgata atggtgcccc
      901 gatcttccaa gggcgagagc tcggccttca gcgcggtgaa cagatatacg atcgagccag
      961 cggccgccac gcccaggccc acgatcagcc aacgacgggt cagcgaacgg gtcagcaggc
     1021 cgcggtaccc ggtgttgagc cgttcgattc cgcgctcgat catcccgtac aaacgcccgt
     1081 gctgacgttc atggcgcagg agcttcgcgc acatcatggg cgtcaaggtc agagccacaa
     1141 aacccgagac cagcaccgcg gccgataccg tgagcgcgaa ctcgcggaac agccgaccgg
     1201 tggtaccggt catgaacgcc acgggcacga acaccgcggc cacggtgatg gtcatggcca
     1261 ggaccgcgaa cgcaatctcg cgactgccct cgagcgctgc ctgccacggc gccatgccgc
     1321 gttcgatgcg gcggtagatg ttctccagca tcacgatggc gtcgtccacc accagcccga
     1381 tcgccagcac cagcgccagc agcgtgagga cgttcacgct gaagcccagg gcgtacacga
     1441 agatgaacgc cccgaccaat gagaccggaa tggtcacgaa cggtatcaga gtggcgcgcc
     1501 cggaccggag aaagtagaag atcaccaaca ccaccagcgc caaggcttcg gccagggttt
     1561 caaacaccga cttgatcgac tcgtcgatga agatcgagct gtcgaatccg atctggagct
     1621 tcatgccttc gggcagcccc gcggtcaaac ccggcaacgc ggctttgacc gccttggcca
     1681 ccgccagcgt gttggcgttg gactgtttga ccacccccac gcccaccgcg ggattcccgt
     1741 tgacgcgcac cgcgttgcgg tcatcggcgg cgccgagttc cgcgtgcccc acgtccgcga
     1801 gccgcaccgg gtagccgttg acctccctga tgatgagccg attgaactcc gcgggggcgc
     1861 gcaggtcggt ttcggtcagt acggtgaatt cgcgctgccg gctttcgatc cggcccgaag
     1921 gcacctgcac gttttgactg cgcaatgcgg cttcgacatc ttgcggggtc aggccgtacg
     1981 cggccaaccg ttcgcgatcc agccacagcc gcatggcata ccggcgttcg ccgccgatga
     2041 tcacgctcgc cacgcccggc agcgtctgca accggtcctt gaccacgcgg tccgcgtagt
     2101 cggtgatctc caacggcgag tggcggtcgc tggagaacgc gacccagatc acgggaaacg
     2161 cgtcggcctc gatcttggcg atcaccggct cgtcggcctc ttcgggcaac agcccgcgca
     2221 cgcgcgcgac ccggtcgcgc acgtcgttgg ccgcggcgtc gatgtcgcgc tccaacgtaa
     2281 attccaccgt gatctggctg acttcttcgc ggctgatgga tttgatcgtg cggatgccct
     2341 caatgccggc cagggagtct tcgagcggct gagagatctg actctcgacc acgtcggcgc
     2401 tcgcgcccgt gtacacggtg cgcaccgaca cgatcggcgc gtcgatgttg ggatactcgc
     2461 gcaccgccag acgcgtgtac gagatgatgc cgatcaggat cagcatcaaa ctcatcacgg
     2521 tcgcgaggac cggtcgccgg atggagagtt cggggagaaa cacgtcagcc tttgggcggc
     2581 gaagccccgg cgccgggccc agacggcgcg ccttggttga tgacggcgac cggcgcgccg
     2641 tcgcgaatct tcatctgccc gcccgtcacc acgaggtcgt cggcgttcag cccggacatg
     2701 atttccgcct cgccggccgc ccgccggccg ataaccactt tcaccatcgc ggctttgccg
     2761 tccaccacgc gaaacacgaa cgcgtcgttg cccatcggca ccacggcctg ctccgggatc
     2821 agaatcgcgt tggcgcgttc gccgatgatc acggccaccc gcgcgaacat gcccgcccgg
     2881 agctcgccgc gcgggttcgg aacccgcgcc cgcaccatgg cggtccgcgt ggtttcgtcc
     2941 agacgcggat caacggcgac gattgttccc gtgaacgccc ggtccgggaa cgcgtccacc
     3001 cgaaccgaca cggattggcc gaccccgacc ttgccgaccc cggcttccgg cacccggaaa
     3061 tcgaccttga ccacggagac atcttccaag ttgaccaccg cctggcccgg ctgcaggtag
     3121 tcgccggggc tcacctgacg gagacccaac gttccgccga agggcgcgcg gatcgtgggc
     3181 atcgacttta ccgagggccg agcggccaag gccggtgacg tgctcgtgcg gctggatggc
     3241 acggaactcg ccgcgcaggt ggcgcaaagc gaggccacgg tcgagctggc tcgg
//
LOCUS       DBNGTT010000056         6207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2566296, whole genome shotgun sequence.
ACCESSION   DBNGTT010000056 DBNGTT010000000
VERSION     DBNGTT010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2566296"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1887)
                     /locus_tag="AC16BC_01290"
     CDS             complement(<1..1887)
                     /locus_tag="AC16BC_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3240274.1"
                     /translation="MTPPLGLAGRIARAFVSSRLTPLSMIAALLLGAVAIMVTPREEE
                     PQIVVPMADVFIPFPGASAGEVEQQLTNPIERKISEIRRVEYVYSISRPEMSLIIVRF
                     RVGEDLEKSLVDLYDKLMANQDLIPGGAGPFQVKPRDVNDVPIVAITLWSERYGDDQL
                     RRVADHVLEEIKKVPHTSGGFVAGGRPRRLRIELDPSRLAAQGLTPVDVAERVAGANQ
                     QRIVGRLQAGNREMVVEAGGFLGSRDAFEELVLRASRDGVVHLRDVAAVRDDAEDVSH
                     YTWFGAGPAAMPFVDAAPDRVYPAVTVAVAKQPGANAVSVAAEVIATVERLKGTVIPS
                     DVHVTVTRDYGATANDKSNELLEHLLIAVVAVVLFLGFALGVREAGVVALAIPLTLGL
                     TLFVSLLIGYTINRVTLFALIFAIGILVDDAIVVVENIYRHLRLGRQPPLQAAITAVD
                     EVGNPTILATLTVIAALLPMAFISGLMGPYMRPIPINASIAMFASLLVAFIVVPWFCR
                     VCHRDGAQSGRHNEFEAIESGRLYRIYQRFLTPLIERRGLRLAFFLIIIGLLLGSVWL
                     FVNRSVVAKMLPFDNKSEFEIVIDMPEGTTLETTADAARSLGRFVAIIPEVTDYQIYV
                     GTPAP"
     gene            complement(1884..2930)
                     /locus_tag="AC16BC_01295"
     CDS             complement(1884..2930)
                     /locus_tag="AC16BC_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3240275.1"
                     /translation="MLRRRVLIAAAVAAAAIIGYWWWTGNRGEGPQERERPTVTGETF
                     VATVGEVPRVVEGTGVVVSAREAVLAGKVLAAVKTINAREGARVAAGDVLITLDDREL
                     VADVERAEAERRNAQSRLERLRSVGAEGLVAPQTLEDAERSLRVADAARESAAALLAS
                     AVIRAPFHGVVTDRYIEVGDMATPGKPLLKLEDSRDLRLEVTVAGDEASLIQARQPVE
                     AVIDALGPQPLAGTVAVVVPRAEAATQSITVKIDLPRAPGLQGGLYGRARFAVGRDSA
                     LTVPRSAVSSVGALDRVYVAGADHVLRARLVRLGRAYGDRVEVRAGLEPGERVLADGS
                     RGVDGARFGGAPGP"
     gene            complement(2924..3121)
                     /locus_tag="AC16BC_01300"
     CDS             complement(2924..3121)
                     /locus_tag="AC16BC_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR3240276.1"
                     /translation="MDVNAWLRAIAGAVVLASVGLSVAHDPRWLWLTAFVGLNLLQSS
                     LTNWCPMMTILRKAGVKDGRC"
     gene            complement(3197..3469)
                     /locus_tag="AC16BC_01305"
     CDS             complement(3197..3469)
                     /locus_tag="AC16BC_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3240277.1"
                     /translation="MTRPAPRTVRPCLKRLGKLSAYLDRELPAAACKEIQKHMAGCAD
                     CRVVLKTLKKTIALCRQAPAKPPSRAALARVLSAVKRDLAKSRPKP"
     gene            complement(3466..4014)
                     /locus_tag="AC16BC_01310"
     CDS             complement(3466..4014)
                     /locus_tag="AC16BC_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3240278.1"
                     /translation="MARVKAGDSAAFERLFARHAPRIHRFASRMCRQAQDAEDILQDT
                     FLAAYRHLDTFRGAARFTTWLYTIAANSCRRTRRHSGAIATIDDHGGNGERHLAVDDW
                     TRRPDVQAMRKETRRAIDEAVAALPKDQRLVLVLRDMEGLPAEEVGRIVGASVPAVKS
                     RLHRARLAVRNELTRRFRERSP"
     gene            complement(4315..5121)
                     /locus_tag="AC16BC_01315"
     CDS             complement(4315..5121)
                     /locus_tag="AC16BC_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3240279.1"
                     /translation="MTMATVGDLVPERLPVVPGKTRVRRAIDVMVEAGADAAVVTEDD
                     GSPVGLLTETDIVRRVIATDRHPFATPVEHVMSAPVVAVDAGCALDAAARLMADRGIR
                     HLGVTRGGRAVGWVNAQAVVAAGGVAPVRVRDTMNRLLATIHLRENVREAAERMLESS
                     VGLLLVGGRRARPRLGGWRGAGQDDLAGLLTETDLVVRVLGADRYPYVAEAGEVMTSQ
                     FITIDVEEELATAAEQLIRHDVRHLVVTAGQEVVGVLSIRDLLGATFVPS"
     gene            complement(5144..5974)
                     /locus_tag="AC16BC_01320"
     CDS             complement(5144..5974)
                     /locus_tag="AC16BC_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3240280.1"
                     /translation="MRPVKEIMATNVQWVKPGTMVRAAAGVMARERIGSVLVGDGSSA
                     IGMVSETDVVRRVLADDRDPTSVTVDEIMSGPVVTVEGHRPIEHAGDLMAEHEIRHVG
                     VTVGGKVAGMVSVRDLLPVVNLLPITVERMMTRMPVLVPTLETVRNAAALMAHACVSG
                     LLVSGERLRPRGIHFRGFTRADIAGIVTGTDLVRDVIDKDLDPYVTPVSAVMGGPLYT
                     LERTESLATAFDVMARGKVRHLGVTSGGEGVTGLLSVEDVIEPAWLHVASGARRKSKG
                     "
BASE COUNT         1048 a         2256 c         1960 g          943 t
ORIGIN      
        1 cggcgcgggc gtgcccacgt agatctgata atccgtgacc tcggggatta tcgccacgaa
       61 acgtcccagc gatcgggcgg cgtcggccgt ggtctcaagg gtcgtgccct cgggcatgtc
      121 gatgacgatt tcaaattcgc tcttgttgtc gaacggcagc atcttggcga ccacggagcg
      181 gttcacgaag agccacaccg accccagcaa caatccgatg atgatcagaa agaacgcgag
      241 ccgcagaccg cgccgctcga tcaacggggt gaggaatcgt tgatagatcc ggtacaacct
      301 gccggactcg atggcctcga actcgttgtg acgtccgctc tgcgcgccgt cgcgatggca
      361 gacccggcag aaccacggca ccacgatgaa cgccaccagc agcgacgcga acatggcgat
      421 cgacgcgttg atcggaatcg gccgcatgta cggtcccatc agcccggaga tgaacgccat
      481 gggcagcaac gcggcaatga cggtcaaggt ggccaggatc gtggggttgc cgacttcgtc
      541 caccgccgtg atcgcggcct ggagcggcgg ctgccggccc agccgcaggt gccggtagat
      601 gttctccacc accacgatcg cgtcgtccac caggatgccg atcgcgaaaa tcagcgcgaa
      661 caacgtgacc cggttgatgg tgtagccgat caacaatgag acgaacaggg tgagcccgag
      721 cgtgagcggg atcgccagcg ccaccacccc ggcttcgcgg acacccaggg caaatcccaa
      781 gaatagtacg accgccacca ccgcgatcag cagatgttcg agcaactcat tggacttgtc
      841 gttcgcagtg gcgccgtagt cgcgggtcac ggtaacgtgg acgtctgatg gaatgaccgt
      901 gcctttgaga cgctccacgg tggcgatcac ctccgccgcc acgctgaccg cgttggcgcc
      961 gggttgcttg gcgacggcca cggtcaccgc gggatacacc cgatccgggg ccgcatcgac
     1021 aaagggcatc gcggcgggtc cggcgccgaa ccaggtgtaa tgggacacgt cttcggcatc
     1081 atcacgcacc gcggccacgt cgcgcaggtg caccacgccg tcgcgcgagg cgcgcagcac
     1141 gagttcctcg aaggcgtcgc gcgatcccag gaacccgccg gcttcgacca ccatctcccg
     1201 gttcccggcc tgcagccgtc ccacgatgcg ttgctggttc gcgcccgcga cccgttccgc
     1261 cacgtcgacc ggcgtgagcc cctgagccgc gagccgagag ggatcgagtt cgatccgcag
     1321 ccgtcggggg cgcccgcccg cgacaaaccc gccggaggtg tgcgggactt tcttgatctc
     1381 ctccaggacg tggtccgcca cccggcggag ctgatcgtcg ccgtagcgtt cgctccacaa
     1441 cgtgatggcc acgatcggca cgtcgttgac gtcgcgcggt ttgacctgaa acggccccgc
     1501 gcctccgggg atcagatcct ggttagccat caatttgtcg tataggtcca ccaggctttt
     1561 ttcgagatct tcgcccaccc ggaaccggac gatgatcaga ctcatctccg gccgggagat
     1621 cgaatacacg tattcgaccc ggcggatttc ggagatcttg cgttcgatgg ggttggtcag
     1681 ttgctgctcg acttcgccgg ccgacgcccc ggggaacggg atgaacacgt cggccatggg
     1741 cacgacgatc tggggctcct cttcgcgcgg cgtgaccatg atggcgaccg cgcccagcaa
     1801 cagcgcggcg atcatggaca acggggtgag gcgcgaggag acgaacgcgc gggcgatccg
     1861 cccggcgagc ccgagcggcg gggtcatggc cctggcgcgc ccccaaagcg cgcgccgtcc
     1921 acgccgcggc tcccgtccgc caggacccgc tcgcccggct ccaatccggc gcgcacttcc
     1981 acacggtcgc cataggcgcg gcccaggcgc acgagccgcg cgcggagcac gtggtccgcg
     2041 cccgccacgt acacgcggtc cagcgcgccc acggacgaca cggccgacct cggcacggtg
     2101 agcgccgagt cgcgtcccac ggcaaaacgc gcgcggccgt acaacccgcc ctggagtccc
     2161 ggcgcgcgcg gcagatcgat tttcacggtg atggattggg tggcggcttc ggcgcgtggc
     2221 acgaccaccg ccaccgtgcc tgcgaggggc tggggaccga gcgcgtcgat caccgcctcc
     2281 acgggctgac gcgcctggat gagactggcc tcgtcgcccg ccaccgtgac ttccagacga
     2341 agatcgcgac tgtcctcaag tttgagcaat ggcttccccg gcgtggccat gtcgccgacc
     2401 tcgatgtagc ggtcggtgac cacgccgtga aacggcgccc ggatcacggc ggaggcgagc
     2461 aaggccgcgg ccgactcacg cgccgcgtcg gccacgcgca acgaccgctc cgcgtcttcc
     2521 agggtctggg gcgcgaccag gccctccgcc ccgaccgacc gcaggcgctc caggcggctc
     2581 tgggcgttcc ggcgctcggc ctccgcgcgc tccacgtccg cgaccagttc gcgatcatcg
     2641 agcgtgatga gcacgtcccc ggccgcgacc cgcgcgccct ccctggcgtt gatggtcttg
     2701 accgcggcca ggactttccc ggccagcacg gcttcgcgcg cggacaccac cacgcccgtg
     2761 ccctccacca cgcgcggcac ctcgcccacc gtggccacga acgtctcccc cgtcaccgtg
     2821 ggtcgctcgc gctcttgcgg cccttcgccg cgattgccgg tccaccacca atacccgatg
     2881 atcgccgcgg ccgccaccgc cgcggcaatc aaaactcgcc gtctcagcat cgtccgtcct
     2941 tgactcccgc cttgcgcaag atcgtcatca tggggcacca gttggtgagc gacgactgga
     3001 gcagattcag gcccacgaac gcggtcagcc acaaccaccg cggatcgtgc gccacgctca
     3061 accctacgct cgccaacacc accgctccgg caatggcccg taaccacgca ttgacgtcca
     3121 gtttcatatt cagttcctcc cagggtgaat gagcaccgcc ttcttcccct gtagagtcac
     3181 gaaccggaaa aaggattcag ggcttcggcc gagacttggc caggtcccgc ttgaccgcgg
     3241 acaacacccg tgccaatgcc gcgcgcgagg gcggcttcgc gggagcctga cggcacaggg
     3301 caatggtttt cttcagtgtt ttcaatacga cccggcaatc cgcgcaaccc gccatgtgct
     3361 tttggatctc cttgcacgcc gcggccggca attcgcgatc aaggtacgcg gacaatttcc
     3421 ccaggcgttt cagacacggg cggaccgtgc ggggcgcggg acgggtcatg gtgagcgctc
     3481 ccgaaaccgg cgggtcaact cgttgcgcac ggccagccgc gcccggtgca ggcgcgactt
     3541 gaccgcgggc acgctcgccc ccacgatccg ccccacttcc tcggcgggca ggccctccat
     3601 gtcgcgcaac accaagacca gccgttgatc cttcggcagc gccgccacgg cctcgtcgat
     3661 cgctcgccgc gtctccttgc gcatcgcctg gacatcggga cggcgcgtcc agtcgtccac
     3721 cgcgaggtgg cgctccccgt tgccgccgtg gtcgtcgatg gtcgcgatcg cgcccgaatg
     3781 gcgtcgcgtc cggcggcacg agttggccgc gatggtgtag agccatgtgg tgaatcgcgc
     3841 ggcgccgcga aacgtgtcga ggtgccgata ggcggccagg aaggtgtcct ggaggatgtc
     3901 ttccgcgtcc tgcgcctgcc gacacatgcg ggacgcgaac cggtggatgc gcggcgcgtg
     3961 gcgggcaaag aggcgttcga acgcggccga gtcccccgcc ttgacgcgtg ccaccagagc
     4021 ccggtcccca tcctccacgg gaatcttcta gcagagtccc ctacgggatt tcaaccgaac
     4081 acgtcgattc aaccgaaatc cttcaatcag gatactcaca gagtctgagg aatttgcagg
     4141 ttgctcaaaa agctccagcc gcaaggccgc agcgaggagg aaaccgcagc gtcctgggtc
     4201 gtacgtgagg atttccgacg agcgagaatc cgcaccgctc cgaaggagcg ggtggaaatc
     4261 tccatcctcg cggatcgcga agcaacgcag cggatggact ttttcagcaa cctgctagga
     4321 cggcacgaac gtcgcgccca ataggtcgcg gatcgacagc acgccgacca cctcctggcc
     4381 cgcggtcacc accaggtgcc gcacgtcgtg gcggatcagt tgctccgcgg ccgtcgccag
     4441 ttcctcttcc acgtcgatcg tgatgaactg cgacgtcatg acttcccccg cctcggccac
     4501 atacgggtag cgatccgccc ccagcacccg gaccaccaga tccgtctcgg tcaacagccc
     4561 ggccagatcg tcctgtccgg cgccgcgcca cccgcccagg cgagggcggg cccggcgccc
     4621 gcccaccagc agcagcccga ccgacgattc gagcatgcgc tccgcggcct cgcggacgtt
     4681 ctcacggaga tgaatggtgg cgagcagacg gttcatggtg tcgcggaccc ggaccggggc
     4741 caccccgccg gccgcaacca cggcctgcgc gttcacccag cccacggcgc gtcctccgcg
     4801 cgtcaccccg aggtgacgaa tgccgcgatc cgccatcaag cgcgccgccg cgtcgagggc
     4861 gcagcccgcg tcgaccgcga ccacgggcgc gctcatcacg tgttccaccg gcgtggcgaa
     4921 cggatgccgg tcggtcgcga tcacgcggcg cacgatgtcc gtttccgtga gcaggcccac
     4981 cggcgatccg tcgtcctcgg tcaccaccgc ggcgtccgcg ccggcctcga ccatcacgtc
     5041 gatcgcgcgc cggaccctgg tcttgccggg aacgaccggg agccgctccg gaacgaggtc
     5101 gccgaccgtt gccatggtca ttccacgcct ccgcggcgcc ggatcagccc ttggattttc
     5161 gtcgggcgcc ggacgccacg tgcaaccacg cgggctcgat cacgtcttcg accgacagca
     5221 gaccggtcac cccttccccg cccgaagtca cccccaggtg gcggaccttg ccgcgggcca
     5281 tcacgtcgaa cgcggtggcc aacgactcgg tgcgttcgag cgtatacaac ggaccgccca
     5341 tcaccgcgct gaccggggtc acgtagggat ccaggtcctt gtcgatcacg tcgcggacca
     5401 gatcggttcc ggtgacgatg cccgcgatgt ccgcgcgcgt gaatccgcga aagtgaatcc
     5461 cgcgcggccg caagcgttcc ccggacacca ggaggccgct cacgcacgcg tgcgccatga
     5521 gcgcggccgc gttgcgcacg gtctccagcg tcggcaccag caccggcatc cgggtcatca
     5581 tgcgctccac cgtgatcggc aacaggttca cgaccgggag caggtcccgc accgagacca
     5641 tgcccgccac cttgccgccg accgtgactc ccacgtggcg aatttcgtgc tccgccatca
     5701 ggtccccggc gtgttcgatc ggccggtggc cctccaccgt gaccaccgga ccgctcatga
     5761 tctcgtccac ggtgaccgag gtcgggtcgc ggtcgtccgc cagcacgcgg cgcaccacgt
     5821 cggtttcgct gaccatgccg atcgcgctgg acccgtcgcc caccagcacg ctcccgatcc
     5881 gctcccgggc catcaccccc gccgcggccc gcaccatggt gccgggcttc acccactgca
     5941 cgttcgtcgc catgatttct ttgaccggtc gcatgatgac tccttctcgt gtgagccgcg
     6001 ggcccatgtc ccatccgagc ccccggcgca ccataccgcc agcagcctac ccggtgtcaa
     6061 gtcttcgtac gatgccgaag aggaagtacc gggacgggag caaagggatg aggcggatcc
     6121 ccctcgaaag agaggggctc ggcgtcgagg cgagagcgcc cgcacgccgc tcctgaatca
     6181 ggacgtcagg gagtctgtgt cggaggg
//
LOCUS       DBNGTT010000057         3386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2056163, whole genome shotgun sequence.
ACCESSION   DBNGTT010000057 DBNGTT010000000
VERSION     DBNGTT010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2056163"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="AC16BC_01325"
     CDS             complement(<1..1247)
                     /locus_tag="AC16BC_01325"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240281.1"
                     /translation="MKFDLGPFTSLVRPHRGAMAQASVMMMGVAALNLALLWLFRYFF
                     DHVLVRHDTTALWRIIWAALGLFALHSALSLRQHIVLTRVGQRIVTSLRLRLVEHLTT
                     LSLDFFVKRRTGELMSRVTGDVGTIQQVATNVPVDLAKQTVTFVGALGLLLYMNWRLC
                     LAILAVVPLVVLVARIFGRRLKALSTEAQDRTAESSTILEEMISGIKLVKSFVMERHE
                     VDRLGAHLARADEVAARRAALVGVFVPVITVLTLLGALGVLWYGGVQVIAGAMTPGDL
                     VAFLLYGGILMGPFSAVARVFTQIKEAQGAMQRVTQILNERPTVRDTVGAAPLGVVRG
                     RVEVRDVTFAYEPGRPVLRGVTLTAEPGQIVALVGPSGGGKSTLVHLLHRFYDPETGA
                     ILLDGRDLRTIRLEELYARIAFVP"
     gene            complement(1244..1546)
                     /locus_tag="AC16BC_01330"
     CDS             complement(1244..1546)
                     /locus_tag="AC16BC_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240282.1"
                     /translation="MSALPSSPSPTPEFVEITILDRRYRVRSDVSLVWVLYDEGYIAA
                     SNRFCWNGECLYCMVTVILPDTTVAVRKKACEVYPTAGLNVIRLGGEFCAPSRLAS"
     gene            complement(1588..2559)
                     /locus_tag="AC16BC_01335"
     CDS             complement(1588..2559)
                     /locus_tag="AC16BC_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR3240283.1"
                     /translation="MSGLIKSPRVASPTAPTGPGEPRQGVPLTDSAIAALVTLLERES
                     KAENLVIIRSHLEDLGETALPWLDAAASTEDGCVAAADMARCIRRRVLDHEWDRWVAD
                     PEAALETGAILIDRFGDPGVAPDAVGRHLDDLAAGLGRRLPGAPDTAETIERLNEFLF
                     SELGFRGNTESYDDPDNSYLSKVLDRRVGIPVSLSVVVLLLAKRLHLPIVGIGMPGHF
                     LLRYGADPSGPYLDPFGNGRVLSREECIAWLRASGFTFAPGMLHPVGPRYIVARMLRN
                     LVAVFTKRGASSEVDILARYLKRVIAAPESVTGTPSESLSETDSESA"
     gene            2646..>3386
                     /locus_tag="AC16BC_01340"
     CDS             2646..>3386
                     /locus_tag="AC16BC_01340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3240284.1"
                     /translation="MATVLNDQPIRTLFADALSTRVFPGGVLLVDVGGRIAFFDAFGR
                     TSNAHASAAVTTDTVFDLASLTKPLVTAALVCSLAAERRISIDDPVGRYLPGWREGPA
                     AGATLRHLLCHAAGLPDWRPFYRQLPPELVATADGKRRILDAAEREPLASAPGSETRY
                     SDLGFMVLGSIVERVMDQPLDEAATQRLVAPTGASAIGYLPVGRTETPRWIAGRAVAA
                     TEQCPWRGRVLCGEVHDENAYAMGGVAGH"
BASE COUNT          613 a         1168 c         1113 g          492 t
ORIGIN      
        1 ggcacgaacg cgatccgggc gtacaactcc tccagccgaa tcgtccggag atcacgcccg
       61 tcgagaagga tcgcgcccgt ctcggggtcg taaaatcggt gcaggaggtg gaccagcgtg
      121 gatttgcccc cgccgctggg cccgaccaat gcgacgatct gtccgggctc ggcggtcagg
      181 gtgacgccgc gaagcaccgg gcgaccgggc tcgtaggcga acgtcacgtc ccgcacctcc
      241 acgcggccgc gaaccacgcc caacggcgcc gcgccgacgg tgtcgcgaac ggtcggccgt
      301 tcgttgagaa tctgggtcac ccgctgcatc gcgccctgcg cttccttgat ctgcgtgaac
      361 acgcgcgcga ccgcggagaa cggccccatc aggatccctc cgtacagcag gaacgccacc
      421 aagtcccccg gagtcatggc gcccgcgatc acttgaaccc cgccgtacca caacacgccc
      481 agggccccga gcagggtgag cacggtgatc accggcacga acacccccac cagcgcggct
      541 ctccgcgcag ccacttcgtc cgcgcgggcg aggtgcgcgc cgagccgatc gacttcgtgg
      601 cgctccatca cgaaggattt gaccagtttg atcccggaga tcatctcctc cagaatcgtg
      661 gaggattcgg cggtgcggtc ctgcgcctcg gtcgacagcg ccttgaggcg acggccgaag
      721 atcctggcga cgagcaccac cagcggcacc accgccagga tcgccaggca cagccgccag
      781 ttcatataca agagcaggcc caacgcgccg acgaacgtga cggtctgctt ggccaggtcc
      841 accggcacgt tggtggccac ctgctggatg gttcccacgt cgccggtcac ccgggacatc
      901 aattccccgg ttcgccgttt gacgaagaag tccagagaca acgtggtgag gtgttccacc
      961 aggcgcaagc gcagcgaggt gacgatgcgt tggcccaccc gcgtgagcac gatgtgctgg
     1021 cgcagcgaca acgcgctgtg cagcgcgaac aacccgagcg cggcccagat gatccgccac
     1081 aacgcggtgg tgtcgtggcg gaccaggacg tggtcgaaaa aatatcgaaa caaccacagc
     1141 agcgcgaggt tgagcgcggc cacgcccatc atcatcaccg aggcctgcgc catcgcgccg
     1201 cggtgcggac gcaccaatga tgtgaacggg ccgaggtcga acttcacgag gcaagacggc
     1261 tgggcgcgca gaactccccg ccgagccgga tgacgttcaa gcccgccgtc ggatagacct
     1321 cgcacgcctt cttgcgcacc gcgacggtgg tgtcgggcag gatgaccgtg accatgcagt
     1381 acaggcactc gccgttccag cagaagcggt tgctggcggc aatgtagcct tcgtcataca
     1441 ggacccacac cagggacacg tcggacctga cccggtagcg acggtcaaga atcgtgatct
     1501 cgacgaactc gggagtgggc gagggagagg acgggagagc gctcatgtcg gtcgtcgacg
     1561 gattcggcgc atgccgtggt ggacgagtca ggccgactcg gaatccgtct ccgacaagga
     1621 ctccgatggg gtcccggtta cggactccgg cgcggcgatc acgcgcttga ggtatcgggc
     1681 caaaatgtcc acctcggacg atgcgccgcg tttcgtgaac accgccacga gattccggag
     1741 catgcgggcc acgatgtagc gtgggcccac cggatgcagc atcccggggg cgaacgtgaa
     1801 ccccgacgcg cgaagccagg caatacactc ttcacggctc agcacgcgcc cgttcccgaa
     1861 cgggtcgagg tacgggcccg acggatcggc cccgtaccgc agcagaaaat gtcccggcat
     1921 gccgatcccc acgatcggca aatggagccg tttggccagc aacaacacca cgaccgacag
     1981 tgacaccggg atacccacgc gccggtcgag gactttcgac aggtaactgt tgtccggatc
     2041 gtcgtacgac tcggtgttcc cccggaaccc gagttccgag aatagaaact cattgaggcg
     2101 ctcgatggtc tccgccgtgt ccggcgctcc ggggagacga cggcccagcc ccgcggccag
     2161 gtcatccagg tgtcgtccca ccgcgtcggg agcgacgccg ggatccccga accgatcgat
     2221 cagaatggcg ccggtctcga gagccgcctc ggggtccgcg acccaccggt cccattcgtg
     2281 gtcgagcacc cggcgccgaa tacagcgcgc catgtcggcc gcggcgacgc aaccgtcctc
     2341 ggtcgacgcg gccgcgtcca accacggcaa cgccgtctcg ccaaggtctt ccagatggga
     2401 acggataatg acgagattct cggcctttga ctcgcgctcc aacagcgtca ccagcgcggc
     2461 gatggctgag tcggtgagcg gaacgccttg acgcggctcg cccgggcccg ttggggccgt
     2521 tggcgaggcg acgcgtggcg atttgatcag accggacacg ccgctatcat aacacaaggc
     2581 cgtcgcgaag gaccacgcaa cgccgcccgc gaagagcctc cgaatcgcgc gggcggcacg
     2641 ttataatggc gaccgtgctc aacgaccagc ccatcaggac tctcttcgcg gacgccctct
     2701 ccacccgcgt gtttccgggc ggcgtgctcc tggtcgacgt cgggggccgc atcgcgttct
     2761 tcgacgcgtt cggccgtacc tcgaacgctc acgcgtcggc cgccgtgacc accgacaccg
     2821 tgtttgacct cgcgtcgttg accaaaccgt tggtgaccgc ggccctcgtg tgctcgctcg
     2881 cggccgagcg ccggatctcg atcgacgatc ccgtgggccg atacctgccg ggctggcgcg
     2941 agggaccggc cgcgggcgcg acgttgcgcc acctcctttg tcacgccgcc ggcctgcctg
     3001 actggcgccc gttctaccga caactcccgc cggaactggt ggccacggcc gacggcaaac
     3061 gacggattct cgacgccgcc gagcgagagc cgctggccag cgctccggga tcggagaccc
     3121 gctacagcga tctgggcttc atggtgctgg ggtcgatcgt ggagcgcgtg atggatcagc
     3181 cgttggatga ggccgcgaca caacgactcg tggcgccgac cggcgcgtcc gcgatcggct
     3241 atctgccggt cggccggacc gagacgccgc gctggatcgc cggccgcgcg gtggccgcga
     3301 ccgagcagtg tccctggcga ggccgcgtgt tatgcggcga ggtccatgac gaaaacgcgt
     3361 acgcgatggg aggcgtggcg ggccac
//
LOCUS       DBNGTT010000058         3311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2439096, whole genome shotgun sequence.
ACCESSION   DBNGTT010000058 DBNGTT010000000
VERSION     DBNGTT010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2439096"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="dnaN"
                     /locus_tag="AC16BC_01345"
     CDS             <1..1054
                     /gene="dnaN"
                     /locus_tag="AC16BC_01345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3240285.1"
                     /translation="EKRNTMPILSNVLIETAERGVTLFATDLELGIQASYPATVKTAG
                     SATVSARKLFEIVRELPEADIRLSSDDGHAIKVDCARSRFKVSGLAPADFPVFPGVGS
                     DPLLTIARADFLDLVRRTAFAVGENDARYVLNGVLCAVERGTIKLVGTDGHRLAVAQR
                     PLVLSKGAAPLPDTSAIVPKKALVELKKLVEDSDQETLELHISKTQMTFQQGDLVLLA
                     RLMEGNYPNYAQVIPIGNDKRITLDKASLEGALRRVALLSKERTNAVRFQIEPSRVVV
                     STSNPDLGEAKEDIAVAYEGEAITTGFNARYLLDVLGAVGSPEVTLEFKDALSPCVIR
                     EPSVEGFLCVVMPMRV"
     gene            1098..>3311
                     /gene="gyrB"
                     /locus_tag="AC16BC_01350"
     CDS             1098..>3311
                     /gene="gyrB"
                     /locus_tag="AC16BC_01350"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR3240286.1"
                     /translation="MTAQAAARYGAEDIKVLEGLEAVRKRPAMYIGSTGIDGLHHLVY
                     EVVDNSVDESMAGYCDAIKVILHVDGSATVIDNGRGIPTEMHPAQKRSAAEVVLTVLH
                     AGGKFDNEAYKVSGGLHGVGVSVVNALSEWLEVEIRRDGQVFHQHYDRGKPAAPLAVT
                     GKTNKRGTTVTFKPDSQIFETTEFVFDTLAQRLRELAFLNRGLSITLADERTEKEQEY
                     AYKGGIVSFVEHLNENKSPLHKPIYITKEKDRLVLEVALQYNDSYSENLFSFANNINT
                     REGGTHLVGFKAALTRTVNNYASSHNLLKGESITGDDVREGLTAVISAKLPQPQFEGQ
                     TKTKLGNTEVKGAVEAAVNEALGEFFEENPSVAKKIIEKSLNAARAREAARKAKELIR
                     RKNALDSGALPGKLADCQERDPALSELFIVEGDSAGGSAKQGRDRKNQAILPLKGKIL
                     NVEKARFDKMVSSEEIRVLITALGTGIGSEDFDASKARYHKIIIMTDADVDGAHIRTL
                     LLTFLYRQMKELIERGYVYIAQPPLYKIKRGKQERYVKDDEGLKTYLLDAAVEDIEVA
                     AGDRWTGGRALAALLKKLVRYDALVRHFNRRQIPGELIEAVVTGEELTREMLRDAKRL
                     RRVRDEVVERLRRVVGLTAEVDVAADEEHKAHKLVVRYVTSGLRQTLDIDHGFIATGE
                     FRELLTVSPPALGLGSGPYRFREAGREGAAAGPEQTAPTATGLLAAILEHGKKELA"
BASE COUNT          695 a         1012 c         1091 g          513 t
ORIGIN      
        1 cgaaaaacgc aacaccatgc cgatcctctc gaacgtgttg atcgagaccg cggaacgcgg
       61 ggtcacgctc ttcgcgacgg acctggagtt ggggatccag gcctcgtatc cggcgaccgt
      121 caagaccgcc gggtccgcca cggtgtcggc ccgcaagctg tttgaaatcg tccgcgaact
      181 tccggaggcc gacatccggc tctcgagcga cgacgggcac gccatcaagg tcgattgcgc
      241 gcggagccgg tttaaggtct cgggcctggc gccggcggac tttccggtct tccccggcgt
      301 gggttcggac ccgctgctga cgatcgccag ggccgacttt ctcgacttgg ttcggcggac
      361 cgcgttcgcg gtcggcgaaa acgacgcccg ctacgtgttg aacggcgtgt tgtgcgcggt
      421 ggagcgcgga accatcaaac tcgtcggaac cgacgggcat cgattggccg tggcgcaacg
      481 gccgctcgtg ctgtcaaaag gcgccgcccc gcttcccgat acgagcgcga tcgtccccaa
      541 aaaagctctc gtcgagctca agaagctggt tgaagactcc gaccaggaaa cgttggaact
      601 ccacatcagc aagacgcaga tgacgtttca acaaggagac ctggtcctcc tcgcgcgact
      661 catggagggc aattacccga actacgccca ggtgatcccg atcggcaacg acaaacgcat
      721 caccctggat aaagcctctc ttgagggagc gctgcgccgg gtggcgctcc tctccaagga
      781 acgcaccaac gcggtgcggt tccaaatcga accgtcccgc gtggtggtgt ccacgagcaa
      841 cccggacctg ggggaggcca aggaagacat cgcggtggcc tacgaaggcg aagccatcac
      901 caccggattc aacgcgcggt atctcttgga cgtgctcgga gccgtgggga gtccggaggt
      961 gaccctggag ttcaaggacg cgctcagccc gtgcgtgatt cgggagccct cggtcgaggg
     1021 atttctgtgc gttgtgatgc cgatgcgggt gtgagggagc cgatgcgcat gcgcgaacaa
     1081 gggagcgaag gacgtctatg actgcccaag cagcggcccg gtatggagcc gaggacatca
     1141 aggtcttgga agggctcgaa gccgttcgca agcggccggc catgtacatc ggcagcaccg
     1201 gcatcgatgg gctgcaccac ctcgtgtacg aggtggtgga caacagcgtg gacgaatcca
     1261 tggcgggata ctgcgacgcg atcaaggtca tcctgcacgt ggacgggagc gccaccgtga
     1321 tcgacaacgg ccgcggcatc ccgaccgaga tgcaccccgc gcaaaaacga tcggccgccg
     1381 aggtggtgtt gaccgtgttg cacgcgggcg gcaagttcga caacgaagcg tacaaggtct
     1441 ccggagggct ccacggcgtg ggggtgtcgg tcgtgaacgc gctgtccgag tggctggaag
     1501 tggagattcg ccgcgacggc caggtgtttc accagcacta cgatcgcggg aagccggccg
     1561 cgccattggc ggtcacgggc aagaccaaca aacgcgggac cacggtcacg ttcaaacccg
     1621 actcgcagat cttcgaaacc acggagttcg tgttcgacac gctcgcccag cgcctgcgcg
     1681 agctggcgtt tctcaaccgc ggcttgtcca tcacgctcgc cgacgagcgc acggaaaagg
     1741 agcaggagta cgcgtacaag ggcggcatcg tctcgttcgt cgaacatttg aacgagaaca
     1801 agagcccgct ccacaagccc atctacatca ccaaggagaa agaccggctc gtcctggaag
     1861 tagcgctcca gtacaacgac agctacagcg aaaacctgtt ctcgttcgcg aacaacatca
     1921 acacccgcga aggcggcacg catctggtcg gattcaaggc cgcgttgacc cgcacggtca
     1981 acaactacgc gtcgtcccac aatctgctca agggggaatc catcacgggc gacgacgtgc
     2041 gcgaggggct caccgcggtc atcagcgcca agctgccgca accgcagttc gagggtcaga
     2101 ccaagaccaa actcgggaac accgaggtca agggcgcggt ggaggccgcg gtcaacgagg
     2161 cgctgggaga attcttcgag gaaaatccct cggtcgcgaa aaagatcatc gagaaaagct
     2221 tgaacgcggc ccgcgcgcga gaggccgcgc gcaaagccaa ggagctgatc cgccgcaaaa
     2281 acgcgctgga cagcggcgcc ttgccgggca agctcgcgga ctgccaggag cgcgaccccg
     2341 cattgtccga gctctttatc gtggagggcg actccgccgg cggctcggcc aaacagggcc
     2401 gagaccgcaa gaaccaggcg atcctgccgc tcaagggcaa aatcctcaac gtggaaaagg
     2461 cgcggttcga caagatggtg tcgtccgaag agattcgcgt cctcatcacc gcgttgggca
     2521 ccggcatcgg ctcggaagat ttcgacgcga gcaaggcgcg gtatcacaaa atcatcatca
     2581 tgaccgacgc ggacgtggac ggcgcgcaca tccgcacgct cctgctgacg tttctgtacc
     2641 ggcagatgaa ggagctgatc gagcgcggct acgtctacat cgcgcagccg ccgctgtaca
     2701 agatcaagcg gggcaagcag gagcggtacg tcaaagacga cgaaggcctc aaaacgtatt
     2761 tgctggacgc ggcggtggaa gacatcgaag tggcggccgg ggatcgctgg accggcggcc
     2821 gcgcattggc cgcgttgctg aaaaagctgg tgcgatacga cgccttggtc cggcatttca
     2881 atcgccggca gattccgggc gaactgatcg aagcggtggt gacgggcgag gagctgaccc
     2941 gcgagatgtt gcgcgacgcc aagcggctgc gtcgcgtgag ggacgaggtt gttgagcgcc
     3001 tgcgcagggt ggtcggtctc acggcggaag tggacgtggc cgcggacgaa gaacataaag
     3061 cacacaagtt ggtggttcga tacgtcacgt cgggccttcg ccaaacgttg gacatcgacc
     3121 acggttttat cgccaccggc gaattccgcg aattgctcac ggtgtctcct cccgcgctgg
     3181 gcctgggctc gggaccctac cgtttccggg aagctggccg cgagggcgcg gccgcgggcc
     3241 ccgagcagac cgcgccaacc gctaccggcc ttctggccgc gattttggag cacggcaaga
     3301 aggagctggc g
//
LOCUS       DBNGTT010000059         3206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2823050, whole genome shotgun sequence.
ACCESSION   DBNGTT010000059 DBNGTT010000000
VERSION     DBNGTT010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2823050"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="AC16BC_01355"
     CDS             <1..901
                     /locus_tag="AC16BC_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240287.1"
                     /translation="MPGAGHLVHMPAHTYMRVGMYQEAEEANVHAVHVDEGYIRDRKP
                     QGFYPMAYYPHNLHFLYWAAAMAGRSQVAIATARELATKVPDEMVKEVPPLEMFKPIP
                     YFALTLFGKWDDMLNEPEPAAELKYTAGIWRYARGRALTATAKIDEAKAELGAVTKLA
                     DEIPPDQMVNLNSAKSILQLAGKVLAGEIAAKEGRTDDAVQSLTEAIAIEDRLVYDEP
                     RPWYQPVRQNLGAVLLAAKRPAEADKVYREDLTRNPNNGWSLYGLTQSLKARGNKKLA
                     ALEEKYFQKAWSRADVTLTASRF"
     gene            943..2286
                     /locus_tag="AC16BC_01360"
     CDS             943..2286
                     /locus_tag="AC16BC_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HIR3240288.1"
                     /translation="MALDRVRVIIMGAAGRDFHDFNVAFRQDPRYEVVAFTAAQIPNI
                     AGRTYPPSLAGALYPEGIPIYPEAELEFLIESHRIDQVVFAYSDVSHEEVMHAASRVM
                     AYGADFALLGPRSTMIPSTKPVVSVCAVRTGAGKSPTVRAIARLLRNEGLRVVVVRHP
                     MPYGDLAKQAVQRFATREDLLRADCTIEEMEEYAPHLREGTVVYAGVDYEPILRRAEQ
                     EADVILWDGGNNDVPFFTSDLEIVLVDPHRAGHERTYFPGEANLLRAEVLILTKLDTA
                     GPEQIDAVRATIRASNPQAVVIDSSMPVTIDTPELVRGARVLVIEDGPTLTHGGMSFG
                     AGVLAARQAGARELVDPRPAAIGSIADTFARFPHIGNALPAMGYGPDQIRELEDTISR
                     TVCDAVVIATPVDLRQIMTIRQPSCRVTYEFQQQGGPLLREVLGPVIAKARANNA"
     gene            2279..>3206
                     /locus_tag="AC16BC_01365"
     CDS             2279..>3206
                     /locus_tag="AC16BC_01365"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR3240289.1"
                     /translation="MREPLLVALGGNALVKPGQSGSIPEQAAALRASLQGIVELIRRG
                     HPVVITHGNGPQVGHILIRAEVARGQAYDLPLDICVAQSQGETGYLIQQTLHNLLRND
                     GIDRPIAALISRVVVDNNDPRMQRPTKPIGPFYTRDQAVALKARGCSMVDDAHRGYRR
                     VVPSPLPLRIVEAGIMKQLMDGGVIVIALGGGGVPVAMDADGRLAGVEAVVDKDLAAS
                     VLAATIGVTRILDLTSVDHVKLHFGTPAEQNLGRITVADARAYLAAGHFEPGSMEPKI
                     QAAIQFLEQGGQRVVITSPEHAVEGFEGRIGTQ"
BASE COUNT          592 a         1039 c         1075 g          500 t
ORIGIN      
        1 gatgcccggg gccgggcacc tggtgcacat gccggcccac acctacatgc gggtcggcat
       61 gtaccaggag gcggaagagg ccaacgtcca cgccgtgcac gttgacgagg ggtatatccg
      121 ggaccgcaag ccgcagggct tctaccccat ggcctactac ccccacaatc tgcactttct
      181 ctactgggcg gcggccatgg cggggcggag ccaggtggcg atcgcgaccg cgcgagagtt
      241 ggcgaccaag gttccggacg agatggtcaa agaggttcca ccattggaga tgttcaaacc
      301 catcccctac ttcgctttga ccctgttcgg gaaatgggac gacatgctga acgaaccgga
      361 gccggccgct gaactcaagt acaccgccgg catctggcgt tacgcgcgcg ggcgagccct
      421 gaccgccacc gcaaagatcg acgaggccaa ggccgaactc ggcgccgtga ccaagctcgc
      481 cgacgagatt ccaccggatc aaatggtgaa cctgaactcg gccaagtcga tcctgcagct
      541 cgcgggcaag gtccttgccg gcgagattgc ggccaaggaa ggccgaaccg acgacgcggt
      601 gcagtcgctg accgaagcga tcgcgatcga agaccgcctg gtgtatgacg agccgcgccc
      661 ctggtaccaa cccgtgcgcc agaacctcgg agccgtgctc ctggccgcga agcgtcccgc
      721 cgaggcggac aaggtctatc gcgaggatct cacgcgcaac ccgaataacg gctggtcgct
      781 ctatggcctg acgcaaagcc tgaaggcgcg gggcaacaag aagctggcgg ccttggagga
      841 aaaatacttt cagaaagcgt ggagccgcgc cgacgtgacg ctcacggcct cacgattctg
      901 aagccggcct tgggtcatcc caccgagaac gcttcgatac caatggctct ggaccgcgtg
      961 cgtgtgatca tcatgggggc ggcgggtcgg gacttccacg acttcaacgt ggcgttccgg
     1021 caggatccgc ggtacgaggt cgtggcgttc acggccgcgc agattccgaa catcgcggga
     1081 cggacgtacc cgccgtcgct cgcaggcgca ttgtatcccg agggcatccc gatctacccg
     1141 gaggcggagt tggagtttct gatcgaaagc caccggatcg atcaggtcgt gtttgcatac
     1201 agcgacgtgt cgcacgaaga ggtcatgcac gcggcgtcgc gcgtcatggc ctacggcgcg
     1261 gactttgcgc tgctgggccc gcgctccacc atgatcccgt cgaccaaacc cgtggtgtcg
     1321 gtctgcgcgg tcaggaccgg cgcgggcaaa agcccgacgg tccgcgccat tgcccggctg
     1381 cttcggaacg agggccttcg cgtggtggtg gtccgtcacc ccatgccgta cggcgatctg
     1441 gccaagcagg cggtccaacg ctttgcaacc cgtgaggacc tcctacgggc cgactgcacg
     1501 atcgaagaaa tggaagagta cgccccgcac ctccgggagg gcacggtggt gtacgcgggt
     1561 gtggactacg aacccattct gcgtcgggcc gagcaggaag ccgacgtgat tctgtgggac
     1621 ggcgggaaca atgacgtgcc gtttttcaca tccgacctgg agattgtgct ggtggacccg
     1681 caccgcgccg ggcatgaacg gacctacttt ccgggagaag ccaacctgtt gcgggcggaa
     1741 gtcttgatcc tgaccaagct cgacacggcc ggcccggaac agatcgacgc cgtgcgcgcc
     1801 accatccgcg cctccaatcc ccaggccgtc gtgatcgact cgtccatgcc cgtgaccatc
     1861 gacaccccgg agctggtccg cggcgcgcgg gtgctggtga tcgaggacgg accgaccctc
     1921 acgcacggcg ggatgtcctt cggcgccggg gtgctggccg ctcgccaggc cggcgcccgg
     1981 gaattggtgg acccgcgccc ggccgcgatc gggagcatcg cggacacgtt cgcccgcttc
     2041 ccgcacatcg ggaacgcgct gccggccatg ggatacggac cggaccagat ccgcgaactg
     2101 gaagacacca tcagccggac cgtgtgcgac gcggtggtga tcgcgacgcc ggtggatcta
     2161 cgccagatca tgacgatcag acaaccgagc tgccgggtca cgtacgagtt tcaacagcaa
     2221 ggcggtccgt tgttgcggga ggtcttggga cccgtgatcg cgaaggcgcg ggccaataat
     2281 gcgtgaaccc ctgctcgtgg ccctgggcgg taatgcgctg gtgaagcccg ggcaatccgg
     2341 gagcatcccg gagcaagccg ccgcattgcg cgcatcgctc cagggcatcg tggaattgat
     2401 ccggcgcggg catcccgtgg tcatcaccca cggcaacggc ccgcaagtgg gccatattct
     2461 gatccgcgcc gaggttgcgc gcggccaggc gtacgacctg ccgctggata tctgcgtggc
     2521 gcagtcgcag ggtgaaaccg gctaccttat ccagcaaacc ctgcacaatc tgctgcggaa
     2581 cgacgggatt gatcggccga tcgcggcgct gatctcgcgc gtggtggtcg acaacaacga
     2641 cccgcggatg cagcggccca ccaaacccat cggcccgttc tatacgcgcg accaggcggt
     2701 cgcgttgaag gcgcgtggat gttcgatggt cgatgacgcg catcgcggct accgccgggt
     2761 ggtgccgtct cccctgccgc tccggatcgt ggaagccgga atcatgaaac aactcatgga
     2821 tgggggcgtg atcgtcatcg cgctcggcgg cggcggggtt ccggtggcca tggacgcgga
     2881 cggacgcctg gcgggcgtgg aagccgtggt ggacaaggac ctggccgcga gcgtgctggc
     2941 cgcgaccatc ggcgtcacgc gcatcctcga tctgacgtcc gtggatcacg tcaaacttca
     3001 cttcggcaca cccgccgaac agaacctggg gcgcatcacg gttgcggacg cgagggcgta
     3061 tcttgccgcg ggccatttcg agcccggcag catggagccg aagatccagg ccgccatcca
     3121 gttcctcgaa cagggcggcc aacgcgtggt gatcaccagc cccgagcacg ccgtcgaggg
     3181 gttcgagggc cgcatcggca cgcaga
//
LOCUS       DBNGTT010000060         1793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1801170, whole genome shotgun sequence.
ACCESSION   DBNGTT010000060 DBNGTT010000000
VERSION     DBNGTT010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1801170"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="AC16BC_01370"
     CDS             complement(<1..168)
                     /locus_tag="AC16BC_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012370761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3240290.1"
                     /translation="MNIHEFQAKQLFTKFNIPVPRGRRADTVAEAVTIADELNAPVTV
                     VKAQIHAGGRGK"
     gene            complement(257..>1793)
                     /locus_tag="AC16BC_01375"
     CDS             complement(257..>1793)
                     /locus_tag="AC16BC_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3240291.1"
                     /translation="PPLPQADKDALLQKFHPDYKADAYRLVKVGPNAGQKTVHEVADL
                     LEADSSVTAADVKSAADYVTDVLIIGGGGAGCAAALTARAAGAKVLLATKLRLGDSNT
                     VMAEGGMQVAVKPEDSPVRHFLDTIKGGHFANDRALLKVLVEEGPAATQWLLSLGVLF
                     DRDDTGNLRVRSGGATSTPRLLTCKDYTGLELMRVLKDAVVNEDVRVVEFAPAVELLS
                     DGSGRCTGAILKNLDNNRLLTVHAKAVILATGGSGRLHIQGFPTSNHFGATGDALPLA
                     YRLGAELAFIDTFQYHPTGAIYPEAMAGLLVTEAIRGAGSQLVNRLGERFINETDTRD
                     VVAASIIRECVEGRGVTTPSGRRGVWLDTPMVEIVNGEGTLDARFPNMRKMFSRYEID
                     IRTQPVLIHPTLHYQNGGVKIGVDGGSTVKGLYVAGEASGGLHGRNRLMGNSLLDIIV
                     FGRRAGQAAATYATTAALGVVTVEHLTRFRRDLKEAGVQPGRVSPRLMPDYVRREETV
                     SHT"
BASE COUNT          279 a          610 c          599 g          305 t
ORIGIN      
        1 cttgccgcgg ccgcccgcgt ggatctgcgc cttgaccacg gtcacgggcg cgttcaattc
       61 gtcggcgatc gtcaccgcct ccgcgaccgt gtcagcgcga cgcccgcgcg gcaccgggat
      121 gttgaacttg gtaaagagtt gtttggcttg gaattcgtgg atgttcatcg agacctcaag
      181 aggaaaaatc cgacaaaaag acgcgtaata ctagcaagtt cgggggcgag ggtcaatcgg
      241 gtcgcgctcg cggacgtcac gtgtggctga cggtttcctc gcgccgcacg taatcgggca
      301 taagccgcgg ggagacgcgg ccgggctgta cacccgcttc cttcaggtcg cggcgaaagc
      361 gggtcaagtg ttccacggtg accacgccca gggccgcggt cgtggcgtac gtcgcggcgg
      421 cctggcccgc gcgccggccg aacacgatga tgtcgagcag cgagttgccc atcagccggt
      481 tgcggccgtg cagcccgccg gaggcctcgc ccgccacgta caggcccttg accgtggacc
      541 cgccgtccac cccgatcttc acgcccccgt tctggtagtg caacgtgggg tggatcagga
      601 ccggctgcgt gcggatgtcg atctcgtacc gcgagaacat cttgcgcatg ttggggaatc
      661 gcgcgtccag ggtgccctcg ccgttgacga tctccaccat cggcgtgtcg agccacaccc
      721 cgcgacggcc cgacggcgtg gtcacgccgc ggccttccac gcactcgcgg atgatcgacg
      781 ccgccaccac gtcgcgggtg tcggtctcgt tgatgaagcg ttcgcccaac cggttgacca
      841 gttgcgagcc ggcgccgcga atggcctcgg tgaccaacaa tccggccatg gcttcgggat
      901 agatcgcgcc ggtggggtga tactggaacg tgtcgatgaa cgccagctcc gcgcccagac
      961 gatacgcgag cggcaacgcg tcgccggtgg cgccgaagtg gttgctggtg ggaaaaccct
     1021 ggatgtgcaa ccgtccgctg ccgcccgtgg ccaggatcac ggccttggcg tgcacggtga
     1081 gcaggcggtt gttgtccagg ttcttgagga tcgcgccggt gcagcgcccg gacccgtcgc
     1141 tgagcaactc gaccgcgggc gcgaactcca ccacccgcac gtcctcgttg accaccgcgt
     1201 ccttgagcac gcgcatcaac tccaggccgg tgtagtcctt gcacgtgagc aggcgcgggg
     1261 tgctggtggc cccgccgctg cggactcgca aattgccggt gtcgtcgcgg tcgaacaaca
     1321 cgccgagcga caggagccac tgcgtggccg cgggcccttc ctcgaccaac actttgagca
     1381 atgcgcgatc gttcgcgaag tgtccgccct tgatcgtgtc cagaaagtgc cggaccggcg
     1441 agtcctcggg tttgaccgcc acctgcatcc cgccctcggc catcaccgtg ttggaatcgc
     1501 ccaggcgcag cttggtggcc agcaacacct tggcgccggc ggcccgcgcc gtgagcgcgg
     1561 ccgcgcagcc cgcgcccccg ccgccgatga tcaacacgtc ggtgacgtag tccgcggcgg
     1621 acttcacgtc cgcggcggtc accgagctgt cggcctccag caggtccgcg acttcatgga
     1681 ccgtcttctg cccggcgttg ggaccgactt tcaccaaccg gtacgcgtcg gccttgtaat
     1741 cggggtggaa cttctggagc agcgcgtcct tgtcggcctg cggcaacggc gga
//
LOCUS       DBNGTT010000061         4623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1801594, whole genome shotgun sequence.
ACCESSION   DBNGTT010000061 DBNGTT010000000
VERSION     DBNGTT010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1801594"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /gene="valS"
                     /locus_tag="AC16BC_01380"
     CDS             <1..1833
                     /gene="valS"
                     /locus_tag="AC16BC_01380"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR3240292.1"
                     /translation="VKIREDLGNLLADKPVKDARTLVVERLEQDGLLLKTEDHRLAIG
                     KCYRCKTIVEPYASPQWFVNINDPAPGKSLAQPAIDAVRNGKIRLIPEGWANNYFGWM
                     NQIQDWCVSRQIWWGHRIPVWYCRACDPAVATTGASVHIGRAAIPILPPSDAAQQEPT
                     RCHRCGKADLVQDPDVLDTWFSSALWPFSTLGWPQRTPELARFYPTSTLVTSFDILFF
                     WVARMIMMGLKFMGDVPFRDVYIHALVRDAEGQKMSKSKGNVIDPLGIMDQYGTDALR
                     FTLAAMASPGRDVKLSEQRIEGYRNFANKLWNASRFVLMNVEGRSTDSSPEHRSLADR
                     WIRARLNHTIDLVTRDLTDYRFDEAANHLYRFVWHEFCDWYIELAKPRLSTDAPEHEA
                     AATRATILDMLETTLRLLHPFMPFITEEIWQKVPHVGDTIVLAPYPQPVAISNEDLGI
                     QEIMDEIITVITTNRTMRSELNVTPAQKLTAGLKTNASTAERIRRHGEGDIIRLARLS
                     DFQAGPDIEKPDSTVAHATPIGEVFVLLQGVDLEKERTRLQKNLREAQAELLRIDTKL
                     KNPQFTAKAPPEVIADHDRRRAELTEQSRMLSEQLARLGGNGSA"
     gene            1830..2672
                     /gene="nadC"
                     /locus_tag="AC16BC_01385"
     CDS             1830..2672
                     /gene="nadC"
                     /locus_tag="AC16BC_01385"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR3240293.1"
                     /translation="MIATPAVLREFVERALAEDIGRGDATTQALFSDPAPAIGRVSAK
                     NQCVVAGGTVAALVFQTLDRHAAIEYHIEDGQTVLSGDPVLTVRADGRMILTGERVAL
                     NILQRLCGIATVTRSYVDAVRGYATKIVDTRKSPAGWRVLDKYAVRCGGGVNHRFGLD
                     DGILIKDTHLALAGGIETAVRRARVGGRHGLKVEVEVQTLDEVRMALDAGADILLLDN
                     MDLTAIREALALIKGRALVEVSGGVTLAAVRDIAALGVDIVSVGALTHSAPAADLHMD
                     VWPA"
     gene            2669..3469
                     /locus_tag="AC16BC_01390"
     CDS             2669..3469
                     /locus_tag="AC16BC_01390"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR3240294.1"
                     /translation="MSHDAIERGLANPGAWHIQVFDEVTSTNTLALERAAAGASERTV
                     LLADSQTDGRGRAGREWRTPRGAALALSVIFRPGFTNDLWPWLGLAAALATRDAIERV
                     TGIPGLVKWPNDVLINGRKAAGILLETRHVAVSDAESAVVIGIGINVNNRAAAFPEAF
                     RDAATSLLDASGTVTDRNVLAAAILNELRRAVDGLPGGIETMRTRWRSVSATCGAMVA
                     ISTPGGLVEGIDHGLDPQGRLIVRTRDGERTVHTGEVLLCRTAAPLAT"
     gene            3439..4329
                     /locus_tag="AC16BC_01395"
     CDS             3439..4329
                     /locus_tag="AC16BC_01395"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR3240295.1"
                     /translation="MPNRRAPGHLSPMVRRVSRRARPGAAGPILLAIDIGNTNIVVGL
                     FKGKTLALEWRLATHGGRTADEYGLIVRELVRDATTEAPSEAIISSVVPNLTPAFVTL
                     LGRYFGLKPAVVDHTSPLGLAIGYAHPEEIGADRLVNAAAAYNAFGGPLIIVDFGTAT
                     TFCVVSADGTYLGGVIAPGAAISAEALTARAAKLSKVEWVRPPTVIGRDTTSSMQTGM
                     ILGHASMVDGIIHHIQQEIGTRCRVLATGGLTSLIAPASQYIETVRPHLTLEGLALIA
                     DRLRTGWRVSHPGTKRRPRA"
     gene            4457..>4623
                     /locus_tag="AC16BC_01400"
     CDS             4457..>4623
                     /locus_tag="AC16BC_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3240296.1"
                     /translation="MGMDLPSRDQEILRATILCYIKTATPVGSRTLTKSMELGLSPAT
                     IRNVMADLEEMG"
BASE COUNT          907 a         1477 c         1415 g          824 t
ORIGIN      
        1 gtgaagatca gggaggatct cgggaacctc cttgccgata aaccggttaa agacgcacga
       61 actcttgtag tcgaacgctt ggagcaggac ggccttttgc tcaagacaga agatcaccgc
      121 ctggcgatcg gcaagtgtta ccgttgcaaa accatcgttg agccctacgc gtcgccgcag
      181 tggttcgtca acatcaatga cccggccccg gggaaatccc tcgcgcaacc cgcgatcgac
      241 gccgtcagga acgggaaaat ccggctgatt ccggaaggct gggccaacaa ctacttcggc
      301 tggatgaatc agatccagga ctggtgcgtg tcccggcaaa tttggtgggg acaccgcatc
      361 ccggtctggt actgtagagc gtgcgacccc gccgtcgcga cgacaggcgc ctcggttcat
      421 atcggccgcg ccgcgatccc tattctcccg ccatccgacg ccgctcaaca agagccgacc
      481 cgctgtcacc ggtgcggcaa ggcggacctg gttcaggacc ccgacgtcct cgatacgtgg
      541 ttctcctcgg ccctgtggcc gttctccacg ctgggttggc ctcaacgcac gcctgaactg
      601 gctcggttct atccgacctc gaccctggtc acaagcttcg acatcctgtt cttctgggtc
      661 gcgcggatga tcatgatggg cctgaagttc atgggcgacg tgccgtttcg ggacgtgtac
      721 atccacgccc tggtccgcga cgccgaaggc cagaaaatga gcaagtccaa gggcaacgtc
      781 attgatccat tgggcatcat ggatcagtac ggcacagacg cgctgcggtt taccctggcg
      841 gcgatggcct ctccgggacg cgacgtgaaa ctctccgagc agcgcatcga gggatatcgc
      901 aacttcgcga acaaactctg gaacgcctcg cggttcgtgc tgatgaacgt ggagggtcgg
      961 tcaacggact ccagccctga acatcgctca ctcgccgacc gatggatcag ggcccggttg
     1021 aatcacacga tcgacctcgt cacccgtgat cttaccgatt atcgcttcga tgaagccgcg
     1081 aaccatctct accgatttgt gtggcacgag ttctgcgact ggtatatcga actcgcgaaa
     1141 ccccggttgt cgacggacgc cccggagcac gaggccgccg ccacgcgcgc aacgatactc
     1201 gatatgctag agaccacgct gcgcctactg cacccattca tgccgttcat aacggaagag
     1261 atctggcaaa aagttcccca cgtgggagac acgattgtcc tcgctccgta tccgcagccc
     1321 gtcgcgatct cgaacgaaga tcttggcatc caagaaatca tggacgaaat cattaccgtg
     1381 atcacgacca accggacgat gcgcagcgag ttgaacgtga cgcccgccca gaagctcacg
     1441 gctggcctga aaaccaacgc gtcgacggcg gaacgcattc gacgccacgg cgagggcgac
     1501 atcatccgct tggcccgctt atcagatttc caggcggggc cggatatcga gaaacccgac
     1561 agcaccgtgg cccatgcaac gccgattggt gaagtgttcg tactgcttca gggggttgat
     1621 ctcgagaagg aacgaaccag actccagaag aacctccgtg aagcccaggc cgaactgcta
     1681 cgcatcgaca ccaagctcaa aaacccgcag ttcaccgcga aggctccgcc tgaggtgatt
     1741 gcggatcatg atcgccgccg cgcggagctg accgagcaaa gtcgcatgct ctcggagcaa
     1801 ctcgccagac tcggcgggaa cggctcagcg tgatcgcgac gcccgccgtg ctccgggagt
     1861 tcgtggaacg ggccttggcc gaagatatcg gccgcggcga cgccaccacg caggcgttgt
     1921 tctccgaccc cgcgcccgcg atcggccgcg tcagcgcgaa gaaccagtgc gtggtcgcgg
     1981 gcgggacggt cgcggcgctc gtgtttcaga cgctcgaccg acatgccgcg atcgagtacc
     2041 acatcgagga cggccagact gttttgtctg gagatccggt actgacggtg cgcgcggatg
     2101 gccgcatgat cctgaccggc gagcgcgtgg cgctcaatat cctccagcgc ctctgcggca
     2161 tcgcgaccgt aacgcgctcg tacgtggatg ccgtccgagg ttatgccacc aagatcgtgg
     2221 acacgaggaa gtctccggcc ggatggcgcg tcctggacaa atacgcggtc cggtgcgggg
     2281 gcggagtcaa tcaccggttc ggcctcgacg acggcatcct gatcaaggac acccacctgg
     2341 cccttgcggg cggcatcgag accgcggttc gtcgcgcgcg ggtcgggggt cggcatggtt
     2401 taaaagtcga ggtcgaagtg caaaccctgg acgaggtccg tatggcgttg gacgccgggg
     2461 ccgatatcct gctgctcgac aatatggatc tgaccgccat ccgtgaggca ttggcattga
     2521 tcaaaggccg cgcgctcgtg gaagtctccg gcggcgtcac gctggccgcg gtccgagaca
     2581 tcgcggcatt gggcgtcgat atcgtatcgg tcggcgccct cacccactcg gcgccagccg
     2641 ccgatctcca catggacgta tggccggctt gagtcatgac gccatcgaac gcggtctggc
     2701 gaatccgggg gcttggcaca tccaggtgtt cgatgaagtg acctcgacga acacgttggc
     2761 gctggaacgt gcggccgccg gcgcctctga gcggacagtt ctgttggccg attcgcagac
     2821 cgacggccgc gggcgcgcgg ggcgcgagtg gcgaaccccg cgcggcgccg cgctggccct
     2881 gtcggttatt ttccgaccgg gcttcacgaa cgatttgtgg ccgtggctcg ggctggccgc
     2941 cgcgttggcg acacgggacg cgatcgaacg cgtgacggga attcccgggc tcgtgaaatg
     3001 gcccaacgat gtcttgatca acggacgcaa ggccgcagga attttgctgg aaacccggca
     3061 cgttgccgtg agcgatgcgg agagtgccgt ggtcatcggc attgggatca acgtcaacaa
     3121 ccgcgccgcg gcgtttcccg aagcgttccg cgacgccgcc acgtcgctgc ttgacgcgtc
     3181 aggaaccgtg acggaccgca acgtgttggc cgccgcgatt ctcaacgagt tgcgccgcgc
     3241 ggttgacggc cttccagggg gaatcgagac catgcggact cgctggcggt cggtgagcgc
     3301 cacgtgtggc gcgatggtcg cgatttcaac ccccggaggc ctggtggagg gcatcgacca
     3361 cggcctcgat ccccaaggac gactgatcgt ccgcacgcgc gatggcgagc ggaccgtcca
     3421 taccggagag gtgctgctgt gccgaaccgc cgcgcccctg gccacctaag ccccatggtg
     3481 cgtcgcgtca gtcggagggc acgacccgga gccgccggcc caatcctcct ggccatcgac
     3541 atcggaaaca ccaatatcgt cgtcggccta ttcaaaggga aaacactggc ccttgagtgg
     3601 cgtctggcca cacacggcgg tcggactgcg gacgagtacg ggctgattgt ccgcgaactc
     3661 gttcgggacg cgacaaccga ggctccgtcc gaggccatta tctccagcgt cgttcccaac
     3721 cttaccccag cattcgtgac attgctcggc cgctatttcg gattgaaacc ggcagtggtc
     3781 gaccatacat caccgctcgg cctggcaatc ggatacgccc atccggaaga aatcggagcc
     3841 gaccgactgg tcaacgccgc cgcggcctac aacgcgttcg ggggccctct catcatcgtg
     3901 gactttggaa ccgcgaccac gttctgcgtg gtctcggccg atggaacgta tctcggcggc
     3961 gtgatcgcgc ccggcgccgc gatatccgcc gaagccctga ccgcccgcgc cgcaaagctc
     4021 tctaaggtcg aatgggtgcg gcctccaacg gtcatcggcc gcgacaccac gagcagcatg
     4081 cagaccggga tgattctggg ccacgcctcc atggtcgacg ggattattca ccacattcaa
     4141 caggagatcg gcactcgctg ccgcgtcctg gcgaccggcg gcctcacctc gttgattgcc
     4201 ccggcatctc aatatatcga gacggtcagg ccgcatttaa cccttgaggg gctcgccctc
     4261 atcgcggatc gactgagaac cggttggcgc gtcagtcatc caggcactaa gcgacggcct
     4321 cgcgcctgaa ccccgctata acaaccgatc cttctggttc tgttgggaaa cttgacattg
     4381 tcgagacccc attgttaaag tgttgttagc actcaccgat attgagtgct aagcttactt
     4441 gaacaaccac cgggtgatgg ggatggatct tccttctcga gatcaagaaa tcttacgcgc
     4501 gacgatcctc tgctacatca agaccgcgac gccagtcgga tcccgaacac tcacgaaaag
     4561 catggaactg ggcctcagcc ctgccaccat ccgaaacgtg atggctgacc ttgaggaaat
     4621 ggg
//
LOCUS       DBNGTT010000062         5358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3207591, whole genome shotgun sequence.
ACCESSION   DBNGTT010000062 DBNGTT010000000
VERSION     DBNGTT010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3207591"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="AC16BC_01405"
     CDS             complement(<1..783)
                     /locus_tag="AC16BC_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3240297.1"
                     /translation="MLAAMLMPIPSSRQSDNDPPTHADTPQLIGRSDAIRRLAEAVRQ
                     AAETPSPVLIVGEPGTGKDLVAKTLHHLSGRGHGPFIPVDGSSVRDSLLDANTGRPKH
                     WNLAEGGTLYLADIADLSLRAQGELLRALDERTTTGAAPIGSDVRLVVGTHVILEEAI
                     RDGRFRQDLYQRLTAAPILVPPLRDRREDIPVLAAHFLGRISRGHAPGKTIAPEALER
                     LVAYDWPGNVEELFTLIERLAASSGDVIQASDLPPNLRARART"
     gene            complement(791..3454)
                     /locus_tag="AC16BC_01410"
     CDS             complement(791..3454)
                     /locus_tag="AC16BC_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family membrane protein"
                     /protein_id="HIR3240298.1"
                     /translation="MSMRAWSLVALIVLLAVSGGALLSLYVDGLWFGELGYFSVFTTK
                     LWTQVGLAIAVGTTMFAWLAINVWVASRRRLMLLPAPWDTLFAGRAVRPLLLGIAALI
                     AAAQGLQAGGQWLAYLLYTHPVSFGQADPVFGKDLGLYVFTLPFLETIKRWLLIAIGL
                     AAAGSAALYLANQELRVEPARLSVSPAARRHLLWLAAAAALTVAWGFRLDQYHLLYSR
                     RGLVSGALYADVHARLPGLYVMVAVTVASAAILALSANRRGWTLPLAAGAAIPATAIL
                     GGSVLPDVLHRFQVLPNEIVLERPYIEQNIRLTRFAYGLDRIEAKEFPASDQLTAQQV
                     AANPLTTKNIRLWDHRPLLPTYRQLQQIRTYYDFVDVDNDRYVIDGELRQVMISPREL
                     SYRSLPSRIWINEHLTYTHGYGVTLGPVNRVTTEGLPEFFIQNIPPTSSTAIRVTRPE
                     IYYGELPNDYVFVRTNNPEFDYPSGDTNQYTTYHGTGGIPMSSWVRKAAFSAYFGSMK
                     ILLSNDISSDSRVMINRAIHDRAAKLIPFIRYDQDPYLVITGDGRLVWILDGYTTTDR
                     IPYSMPLRGVGNYIRNSVKVTVDAYDGRTTAYISAPDDPLIRAYAAAFPGLFQPIDAL
                     PPDLRAHLRYPQDLFRIQSHVFATYHMQDPQIFYNKEDLWAIPQKGEQDMEPYYTIMK
                     LPGEAREEFILMIPYTPAKRDNMAAWLAARSDGEQYGKLIVYLFPKQKLVYGPRQIEA
                     RIDQDALIYQQLTLWTQRGSQAIRGSLLVIPIEDAILYVEPLYLAASESGSLPELRRV
                     IVSFGNRLVMEPDLEAALARLFGGRSAEARPGARQAEPIAPGLSDPSLGRRALEHFRQ
                     AQRALRDGDWEAYGAELRRVEEALEALAKPP"
     gene            complement(3488..4420)
                     /locus_tag="AC16BC_01415"
     CDS             complement(3488..4420)
                     /locus_tag="AC16BC_01415"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR3240299.1"
                     /translation="MSPVTHSPTAPSRHGHHQRVRLDTSPGFRYTFPRFRITELNGTP
                     RAGKQPGRYRGRTARRMKILSTGSTHVGRVRSSNEDHFGVFDDLGLYIVADGMGGHAA
                     GEVASLMAVQTVREAFEQAAAERTLPASGEYDPGRRLVWAVEQANQRIFNAGTDNPAL
                     SGMGTTVVAVWVDNEWAYIAHVGDSRAYRLHGDRLEQVTSDHSLVNDYLHRGIMTPDE
                     AAVHPMKHVLIRALGTAPGVSVDIRRMRVEPGDLLLLCSDGLSNVVPIEELTSTLAKP
                     SPGDLPARCQALIDAANRRGGLDNITAVLILADK"
     gene            complement(4433..5113)
                     /locus_tag="AC16BC_01420"
     CDS             complement(4433..5113)
                     /locus_tag="AC16BC_01420"
                     /inference="COORDINATES: protein motif:HMM:NF053611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaD family glycine zipper/OmpA-like
                     lipoprotein"
                     /protein_id="HIR3240300.1"
                     /translation="MIHSACRRSVWMAGVALLIAGAASLVGCENMNRTQKGALTGTAV
                     GAGLGALIGKQHGSSGRGALIGGAIGALSGGLIGNYMDRQAEELEKVAATTRTGDGII
                     VTMRDKILFDVDSATIKPAATTSLDKLAAVLVKYPKTTITVAGYTDNTGRPDYNLRLS
                     ERRANAVKFALADRGVATDRIATMGFGPENPVAPNATPDGRAENRRVELHIVPDDTLK
                     HEAAEQDG"
BASE COUNT          971 a         1599 c         1795 g          993 t
ORIGIN      
        1 tgttcgcgcc cgggcgcgga ggttcggcgg gagatcggat gcctggatca cgtcgccgga
       61 gcttgccgcc agtctctcga tcagggtgaa caattcctcg acgtttcccg gccaatcgta
      121 agcgaccagc cgttccaatg cctcgggggc aatggtcttg cccggtgcgt ggccgcggct
      181 gatgcggccg agaaaatggg cggcgagaac gggaatgtct tcgcggcggt cccgcaacgg
      241 cggcaccagg atcggcgccg cggtgagccg ctgatagaga tcttgccgaa aacgtccgtc
      301 ccggatcgcc tcttccaaga tcacgtgtgt gccaacgacc agccttacgt cgctgccgat
      361 cggcgcggcg cccgtggtgg ttcgttcgtc gagagcacgc aacaattcgc cctgcgcgcg
      421 gagactgagg tcggcgatgt ccgcgaggta aagcgtgccg ccctccgcca ggttccagtg
      481 tttggggcgc cccgtattcg cgtccaggag gctgtcccga acggacgaac cgtcaaccgg
      541 gataaacggt ccatggcccc ggccgctcag gtgatgcagt gtcttggcca ccagatcctt
      601 gccggttccc ggttccccca cgatcaggac tggcgaaggt gtctccgcgg cttgtctaac
      661 cgcctcggcg agccgacgga tggcgtcgct gcgcccaata agctgcggcg tgtccgcgtg
      721 agtgggtgga tcgttgtctg attgccgcga tgagggtata ggcataagca tggcggcaag
      781 catagcggcc ctaggggggc ttggcaagcg cctcaagggc ttcttccacc cgcctgagct
      841 ccgcgccgta ggcttcccaa tcgccgtccc gcaacgcgcg ttgggcctga cggaaatgct
      901 ccagcgcgcg gcgaccgagc gacgggtcgg acaaccccgg agcgatgggt tcggcctggc
      961 gcgcgcctgg tcgggcctcg gcgcttcgac cgccgaaaag tctcgcgagt gccgcctcca
     1021 aatccggttc catcaccaaa cggttgccga acgacacgat cacccggcgc agttccggca
     1081 gcgacccgga ctcggacgcc gcgaggtaga gcggctccac atagaggatg gcgtcttcga
     1141 tggggatcac gagcaggctg ccgcggattg cttgggagcc gcgctgggtc cagagcgtga
     1201 gctgctgata gatgagggcg tcttgatcga ttctggcctc gatttgccgc ggcccgtata
     1261 cgagtttctg cttgggaaac aaatagacga tcaatttgcc gtattgctca ccgtccgagc
     1321 gcgcggcgag ccatgccgcc atgttgtctc gtttggccgg cgtgtagggg atcatgagga
     1381 tgaactcttc acgcgcttcg cccggaagct tcatgatggt gtaatacggc tccatgtcct
     1441 gctcgccttt ttgcgggatc gcccagagat cctccttgtt gtaaaaaatt tggggatctt
     1501 gcatgtggta ggtagcgaac acgtgcgact ggatccggaa cagatcctgc gggtaccgca
     1561 ggtgagcgcg gagatccggg ggcaaggcgt caatcggttg gaacagtccg gggaacgcgg
     1621 cggcgtaggc ccggatcagc ggatcgtccg gagcgctgat gtaggcggtc gtccggccgt
     1681 cgtaagcgtc gaccgtcacc ttcaccgagt tgcgaatgta gttgcccacg ccccgcaagg
     1741 gcatcgagta ggggatccgg tccgtcgtgg tatagccgtc gagaatccac acgagccgtc
     1801 cgtcgccggt aatgacgagg tacggatctt gatcgtaccg gataaacggg atgagtttgg
     1861 cggcgcggtc gtgaatcgcg cggttgatca tgacgcgact gtccgaggag atgtcgttgg
     1921 agagcaggat cttcatcgat ccgaagtagg cggagaacgc ggctttccga acccatgaag
     1981 acatggggat gccgccggtc ccgtggtacg tggtgtactg gttcgtatcg cccgaggggt
     2041 agtcgaactc gggattgttg gtccgaacga acacatagtc gttcgggagt tcgccgtaat
     2101 agatctcggg gcgggtcacg cggatggccg ttgatgatgt gggcggaatg ttctgaatga
     2161 aaaactccgg gagtccctcg gtggtgacgc ggttgaccgg ccccagggtg acgccgtagc
     2221 cgtgggtgta ggtgaggtgc tcgttgatcc agatccggct cgggaggctg cgatacgaga
     2281 gttcgcgagg ggaaatcatc acctgccgga gttccccgtc gatcacgtag cggtcattat
     2341 ccacgtcaac gaagtcgtag tacgtgcgga tctgctgcaa ttggcgatac gtgggcagca
     2401 atgggcggtg gtcccagagc cggatgtttt tggtggtgag cggattggcg gcgacctgct
     2461 gtgcggtgag ctgatccgac gcggggaact ctttggcctc gatccgatcc agcccgtacg
     2521 caaaacgcgt caggcgaatg ttctgttcga tgtagggccg ctcgagcacg atctcgttgg
     2581 gcaggacctg gaagcggtgc agcacatcgg gcaagacgga cccgcccagg atcgcggtag
     2641 ccggaattgc cgcgccggcg gcgagcggca gcgtccatcc gcgacgattg gcgctcagcg
     2701 ccaagatcgc cgccgacgcg accgtgaccg ccaccataac gtacaacccc ggcagcctgg
     2761 catggacgtc ggcgtacagg gctccggaca ccaggcctcg gcgggagtac aacaggtgat
     2821 actggtcaag tcgaaagccc catgccacgg tgagcgccgc ggccgcggcc agccagagca
     2881 gatgacggcg tgcggcgggg gagaccgaca accgcgcggg ctccacgcgc agttcctggt
     2941 tcgcgaggta gagggcggcg ctcccggcgg cggccaaacc gatggcgatc agcagccacc
     3001 gtttgatggt ctccagaaac ggcagcgtga acacgtatag gccgaggtcc ttgccgaaca
     3061 cgggatcggc ctggccgaac gagaccggat gggtgtagag caagtacgcc agccattggc
     3121 cgcccgcctg cagcccttgc gccgcggcaa tcagtgcggc aattccgagg agcagcgggc
     3181 gaaccgcgcg tccggcgaac agggtatccc acggagcagg gaggagcatc aggcgtcgac
     3241 gggacgcgac ccacacgttg atggcgagcc aggcaaacat ggtcgtcccg accgcgatgg
     3301 ccaggccgac ctgagtccag agtttcgtgg taaatacgga gaagtacccc agttcgccga
     3361 accacaatcc gtcgacatag agcgacaaca gcgctccgcc ggagacggcg aggagaacga
     3421 tgagggcaac cagactccac gcgcgcatcg acatgggcgt gtctgcttag ggcggtgtca
     3481 ggacgatcta tttatccgcc aggatcaaga cggcggtaat gttgtcgagc cctccgcggc
     3541 ggttcgccgc gtcgatcaag gcttggcagc gcgcagggag atcccctggc gagggcttgg
     3601 ccaaggtcga agtcaattcc tcgatcggaa cgacgttcga aaggccgtcc gaacacagga
     3661 gcaggaggtc gccgggctca acccgcatac ggcgaatatc cacggagacg cccggagcgg
     3721 tcccgagggc gcgaatcaac acatgtttca tcggatgcac cgcggcttcg tccggcgtca
     3781 taattccgcg gtgcaagtag tcgttgacga gggaatggtc cgacgtcact tgttccaatc
     3841 ggtcgccgtg cagcctgtag gcccggctgt cacccacgtg ggcgatgtag gcccactcgt
     3901 tgtcgaccca gaccgccacg accgtggtgc ccatccccga gagcgccgga ttatccgttc
     3961 cggcgttgaa gatccgttga ttggcttgtt cgaccgccca cacgagacgg cgcccggggt
     4021 cgtactctcc cgaggccggg agcgtgcgct cggccgcggc ttgctcaaag gcttcgcgga
     4081 cggtctgcac cgccatgagg ctggcgacct cgcccgccgc gtgccctccc attccgtccg
     4141 cgacgatata gagtcctaag tcatcgaata cgccaaagtg atcttcattg gaactgcgta
     4201 cccggccgac gtgcgtcgac ccggtggaaa ggatcttcat tcggcgagct gttctcccac
     4261 ggtatctccc aggctgtttg ccggcacgcg gcgttccgtt cagttctgta atgcggaagc
     4321 gtgggaaagt gtaacgaaaa cccggtgagg tgtccaggcg gacacgttga tgatgaccgt
     4381 ggcgggatgg cgcagtcggt gaatgcgtca cgggagacat gcgacgaaac ggctatccgt
     4441 cctgttccgc agcctcgtgt ttgagggtgt cgtcgggcac gatgtggagc tcaacccggc
     4501 ggttttccgc gcgcccgtcc ggcgtggcgt tcggcgcaac cgggttctcc gggccgaatc
     4561 ccatggtggc gatgcggtcc gtagccacgc ccctatcggc gagcgcgaac ttgaccgcgt
     4621 tggctcgtcg ctccgagagt cggaggttgt agtccggtcg accggtattg tccgtatacc
     4681 cagccaccgt gatggtggtc ttggggtact tgaccaggac ggccgcgagt ttatcgaggc
     4741 tggtcgtggc cgcgggcttg atcgtcgcgc tgtcgacatc gaaaaggatt ttgtcgcgca
     4801 tcgtgacgat aatgccgtcg ccggttctgg tcgtggcggc gaccttctcc aattcctcgg
     4861 cctggcggtc catataattg ccgatcaggc cgcccgacag cgcgccgatg gcccctccga
     4921 tcagcgcccc acgcccgctc gatccatgtt gtttgccgat caatgctccg aggcccgctc
     4981 ccacggcggt tcccgtgagc gcgccctttt gcgtgcggtt catgttctcg caaccgacca
     5041 gcgacgcggc gccggcgatc aggagcgcaa ctcccgccat ccaaaccgaa cgacgacatg
     5101 ccgagtggat catggaccct cctttattac atttattaca ttcggtaggc ggccgctcag
     5161 ccgcgcaacg agcggtgtag tatacggaaa cggctgtttc ttttgtcaag cgatcgcctc
     5221 caaatcgtgc cggcgactcg gcggacggga tcaacgcggc ggctttacat tcagggaagg
     5281 ttcggatgaa ataggctggc ctggtttgtg gaacggttgg atcatgacgt gactgagcag
     5341 tctggcataa caccggtc
//
LOCUS       DBNGTT010000063         7273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_268950, whole genome shotgun sequence.
ACCESSION   DBNGTT010000063 DBNGTT010000000
VERSION     DBNGTT010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_268950"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="AC16BC_01425"
     CDS             complement(<1..213)
                     /locus_tag="AC16BC_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HIR3240301.1"
                     /translation="MYRIALTLALLIIAGSLPVPAAAADEVVVYSTRIERLISPIFDA
                     YTQETGTRVKFITDTEGPLIARLKAEC"
     gene            complement(320..901)
                     /locus_tag="AC16BC_01430"
     CDS             complement(320..901)
                     /locus_tag="AC16BC_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3240302.1"
                     /translation="MTIVLIVCVLLLFAVIGLGLWIVGLYNELVRMRTAIDQAWANID
                     VVLKQRHDELPKLVEVCNAYMTHERETLEAVTTARAASLAGRKTEEKTQAENRLTAAL
                     GRLFAVAESYPQLLANQTFGQLQQRISAIESQIADRREFFNDAVTVFNTRIQQIPDVF
                     VAQMLQYTPRPLLEIARADAQDVVLSFANRRAA"
     gene            complement(946..1869)
                     /locus_tag="AC16BC_01435"
     CDS             complement(946..1869)
                     /locus_tag="AC16BC_01435"
                     /inference="COORDINATES: protein motif:HMM:NF023899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HIR3240303.1"
                     /translation="MSSGRPPSRGATGSAPPDLSASGRAAVVAWGVVLSFSGLVIALI
                     LIDIVRRSGRPFAWTQTPGEPTLASLAVALSGVAAFGYGVYTRRTKRLIENTPTSPVR
                     SLPLGFVEVAGTAEPDGDPLRSPADQTPCVYYEFRVQEYRGNGSSRKWVTLAEDRSAE
                     PFYLRDETGRVLVVPMGATARIRRAHTFSHTWPDALPDDARLVFQRLNMPTASWLGPR
                     RVRFCERLIGVGDPLYVLGTAQENPAGALHAADTSRLYIGAAPGDRPFLIVDDTEHAL
                     LARLRWISLACLYGGPLASALALGWWLHRIG"
     gene            complement(1949..2905)
                     /locus_tag="AC16BC_01440"
     CDS             complement(1949..2905)
                     /locus_tag="AC16BC_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HIR3240304.1"
                     /translation="MSASRHGIRLTGIGFLAAALSACSISSTGVVKDDTTLTGETERL
                     AESGARYTGPEYTVGIIAFENKTPSKVLGIGEAATTILRTQVEAAGLNAILLDERELK
                     EQKQLADLQQSGAVKTGKKRANEGFDAVDFRLSGAITAYSEVEEGVDAIVYQSKARVA
                     RVTVDYALVDVATGKSLVAESGAGEYRKTTTGALGLGAKSSFDPNLRDGALRDALAKA
                     MNKMVEKLSRQPFVTKVVLADSDSLVIRAGTRSHLSEGTELTVYRPGPEIRDPDSGEL
                     LGSKETRIGVIKISQHQNERLSNATIVSGKGFQTGDIVKAAP"
     gene            complement(2948..3601)
                     /locus_tag="AC16BC_01445"
     CDS             complement(2948..3601)
                     /locus_tag="AC16BC_01445"
                     /inference="COORDINATES: protein motif:HMM:NF017459.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNA1162 family protein"
                     /protein_id="HIR3240305.1"
                     /translation="MVISLTRTATRTTAIIALTLLAGCGGHRAIVPFGPGITPPRTIA
                     LLPLDNQTNSVPGALYMREVMNTQLKDAGYEPQTLEQVDRALSDQFGVSLGGQVTEDM
                     LPKIGTTLGVDAVLTGTIQKFGTVLAVYSEVEATFVMYETGTGRKIWEYHDHVKEDTA
                     LGQRHANSVTLAAGLIASVIERGSGKPLQSVVAQFYRRLTPRLPSGAGALSQNLVAN"
     regulatory      3746..3850
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3965..5878
                     /gene="thiC"
                     /locus_tag="AC16BC_01450"
     CDS             3965..5878
                     /gene="thiC"
                     /locus_tag="AC16BC_01450"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009722406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3240306.1"
                     /translation="MNTHDQDTGTGYAAIAEEVTGRGLTLTPFPASRKIAVEGTRVSV
                     AMREIQVSPTRQAKDGSVEINEPVVVYDTSGPYTDHAATIDIRRGIHPLRHAWIMERG
                     DVEELPRGSSAYGRRREEDPRLDPIRFPNLRKPLRARSDGNVTQMHYARTGLVTPEME
                     YIAIRENQLRERLLDAASGAAPRGHLAHQHPGQGWGASIPKLITPEFVRDEVARGRAI
                     IPANINHPESEPMIIGRNFLVKINANIGNSAVASSIEDEVEKMIWAIRWGSDTVMDLS
                     TGKNIHETREWIIRNAPVPIGTVPIYQALEKVEGRVEELTWDLFRDTLIEQAEQGVDY
                     FTIHAGVLLRYVPLTASRAAGIVSRGGSILAKWCLAHHQENFLYTHFEEICEIMKAYD
                     VSFSLGDGLRPGAIADANDEAQFGELETLGELTRIAWKHDVQVMIEGPGHVPMHLIHE
                     NMTKQLEVCHEAPFYTLGPLTTDIAPGYDHLTSGIGAAMIGWYGCAMLCYVTPKEHLG
                     LPDKDDVKQGVIAYKIAAHAADLAKGHPGAQRRDNALSKARFEFRWEDQFNLSLDPET
                     ARAYHDETLPTEGAKVAHFCSMCGPKFCSMKITQDVRDYAAQKALDDAKAIEAGMTEK
                     SEEFKKAGLEIYR"
     gene            5978..6787
                     /locus_tag="AC16BC_01455"
     CDS             5978..6787
                     /locus_tag="AC16BC_01455"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HIR3240307.1"
                     /translation="MNQTRSIAPTRERDITRAIAREYFRQFDALVESDALIIGSGPSG
                     LVCGRDLALAGFKVVIVEQMNHLGGGFWNGGYLMSKATIAAPAQTILEEVGVPCREVG
                     PEMYVADPPHATAKLIGAAYDAGVKVINFTKVVDLVYRANRLEGVVVNWTPLEMVGHD
                     VAHVDPIALESRVVVDATGHDAVAVQMLAKRGLHQPVPGNGAMWVDQSEEIVIDKTGE
                     VYPNLFLVGLSVAAVYGTPRMGPAFGSMLLSGRKGAGQIAAKLLAASAAAR"
     gene            complement(6919..>7273)
                     /locus_tag="AC16BC_01460"
     CDS             complement(6919..>7273)
                     /locus_tag="AC16BC_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240308.1"
                     /translation="TGSAPDGGPVTFRITRFPDHQDTTKTMSMVQGITYQFIDPITGA
                     QTQAMATSSSTVDETTGPGRGTVSGAPFVIYTPNVCHDIFRTDSFEFVAIDSSGNVSP
                     PATISMYRADITCPH"
BASE COUNT         1323 a         2272 c         2423 g         1255 t
ORIGIN      
        1 gcactcggcc ttgagccgtg cgatcagcgg gccttccgtg tcggtgatga atttcacgcg
       61 cgtgccggtt tcctgggtgt acgcgtcgaa gatggggctg atgagccgtt caatacgggt
      121 gctgtacacc acgacttcgt cggccgcggc ggctggaacg ggcagcgaac ccgcgatgat
      181 gagcagggca agagtcaacg cgatccggta catagaggcc tcatagggga cggtgatgcc
      241 tcaactctac aagaaatgaa aacgatttgc aaaatcattc catggcgcgg gaagaggcgc
      301 ggcgtcgatc catggggaac taggccgccc gccggttggc gaacgacagg acgacgtctt
      361 gggcgtcggc gcgggcgatt tccagcaacg gccgcggcgt gtactgaagc atttgggcca
      421 cgaacacgtc ggggatctgt tgaatgcggg tgttgaacac ggtgaccgcg tcgttgaaaa
      481 actcccggcg gtccgcgatc tggctttcga tcgccgaaat ccgctgctgc aattggccga
      541 aggtctggtt cgcgagcagc tgcgggtacg actcggccac ggcgaacagc cggccgagcg
      601 cggcggtcag gcggttctcg gcctgcgtct tttcctcggt cttgcgaccc gccagcgacg
      661 cggcgcgtgc cgtggttacg gcttcgagcg tctcgcgttc gtgcgtcatg taggcgttgc
      721 agacctccac gagttttggc aattcgtcgt ggcgctgttt gagcacgacg tcgatgttgg
      781 cccacgcctg gtcaatggcc gtgcgcatgc gcaccagttc gttatagagc ccgacgatcc
      841 acagccccag gccgatgacg gcaaagagca ggagcacgca cacgatcagc acaatggtca
      901 tgaccaccct ccttccgaac gcgcataagc gttagacgag accattcatc cgattcgatg
      961 cagccaccaa ccgagcgcca gcgcgctcgc gagggggccg ccgtacaggc aggcgagact
     1021 gatccagcga agacgcgcga gcaacgcgtg ctccgtatcg tccacgatga gaaacggccg
     1081 gtccccgggc gcggccccga tgtacagccg ggacgtgtcc gcggcgtgca gtgcgcccgc
     1141 cggattttcc tgcgcggtcc cgagcacgta cagcgggtct cccacgccga tgaggcgttc
     1201 gcagaaccgg actcgccgcg ggcccagcca gctggcggtc ggcatgttga ggcgctgaaa
     1261 gaccaggcgc gcgtcgtccg ggagcgcgtc cggccacgtg tgggagaagg tgtgcgcgcg
     1321 ccggatccgc gccgtggccc ccatcggaac cacgaggacg cgcccggtct cgtcgcgaag
     1381 gtagaacggc tccgcggacc gatcctctgc cagcgtgacc cacttgcgcg aggacccgtt
     1441 gccgcggtat tcctgcacgc ggaactcgta atacacgcag ggggtctgat ccgcgggact
     1501 gcggaggggg tcgccgtcgg gctcggcggt tcccgcgact tcgacgaagc ccagcgggag
     1561 cgaccgaacg ggagaggtcg gcgtattttc gatcagccgt ttcgtacggc gcgtgtacac
     1621 gccgtacccg aatgccgcga cgccggacag cgccacggcg agcgaggcga gcgtgggttc
     1681 tcccggggtc tgggtccacg cgaatggacg acccgaacgc cggacaatgt cgatcagaat
     1741 cagggcaatg accagcccgg aaaacgagag cacgacgccc cacgccacga ccgcggcgcg
     1801 gccgctcgcg gagagatccg gcggggccga ccctgtggcg cctcgtgatg gaggtcgacc
     1861 ggaggacatc agtcagcccg gagcggcgat actccgaggc cgatgtcggt tgttgctcca
     1921 catcgagccc tcgtgcatgg cgcgctcttc agggcgcggc tttgacgata tcgccggtct
     1981 gaaacccttt ccccgacacg atcgtcgcgt tggagagccg ctcgttctgg tgttggctga
     2041 ttttgatgac gccgatcctg gtctccttgc tgcccaggag ttctccgctg tccggatcgc
     2101 gaatctccgg tcccggccgg tacacggtga gttcggtccc ttccgacagg tgcgagcggg
     2161 tgcctgcgcg gatgaccaga ctgtccgaat ccgcgagcac gaccttcgtg acaaagggtt
     2221 ggcggctgag tttctccacc atcttgttca tggccttggc cagcgcgtcg cggagcgcgc
     2281 cgtcccgcaa attcggatcg aatgacgact tggcgcccag cccgagcgcg cccgtggtgg
     2341 tcttgcggta ctcgccggcg ccggactcgg cgaccagcga cttccccgtg gcgacgtcga
     2401 cgagcgcgta atccacggtg acgcgcgcca cccgcgcttt gctctggtac acgatcgcat
     2461 ccacgccctc ttcgacttcc gaatacgcgg tgatcgcgcc ggagagccgg aagtccacgg
     2521 cgtcgaagcc ttcattggcc cgtttcttcc cggttttcac cgcgccggac tgctgcaggt
     2581 ccgcgagttg cttctgttcc ttgagttcgc gctcatccag taggatcgcg ttaagaccgg
     2641 ccgcttccac ctgggtcctc aggatggtcg tggcggcttc gccgatgccg agcaccttgg
     2701 agggcgtctt gttctcgaac gcgataatgc cgacggtgta ttccggtccg gtgtagcggg
     2761 cgcccgattc ggccagccgt tcggtctcgc ccgtgagggt cgtgtcgtcc ttgaccacgc
     2821 cggtggaact gatgctgcat gccgacaggg ccgcggccaa gaagccgatc cccgtcaggc
     2881 gtattccgtg tcgtgaagca ctcattgccg tctcctttcg atcaagagtc ctcggggttg
     2941 ccgatggtca gttcgcgacg agattctgcg agagcgcgcc cgctcccgac ggcaaacggg
     3001 gggtgagccg ccggtaaaac tgggcgacca cggactgcag gggtttgccg gatccccgtt
     3061 cgatcacgct ggcgatcagc cccgcggcca acgtgacgga gttggcgtga cgctggccca
     3121 gggccgtgtc ttccttgacg tggtcatggt attcccagat tttccgtccg gtgccggttt
     3181 catacatgac gaaggtggcc tccacctcgc tgtacaccgc gagcacggtg ccgaatttct
     3241 ggatcgtgcc ggtcagtacg gcgtcaaccc ccagggtggt cccgatcttg gggagcatgt
     3301 cttccgtcac ttgtcctccc agggagaccc cgaactggtc ggacaatgcg cggtcaacct
     3361 gctccagcgt ctggggctcg tagccggcgt ctttcagctg agtgttcatg acctcgcgca
     3421 tgtagagggc cccgggaacc gagttcgtct gattgtccaa cgggagcaga gcgatggtgc
     3481 gcggcggggt aatcccgggc ccaaacggca cgatggcgcg atggcccccg cacccggcga
     3541 ggagtgtcaa cgcgataatc gcggtcgtcc gcgtcgccgt ccgtgtcaac gagatgacca
     3601 tggtcgatac tcgatccgaa cgaagcaagc ataggggggc tcccggggtt tgtaaagggg
     3661 cgtggctcgc cgcgtcggcg ccgggatgac gggtgtcagt gcccgcattg acttcgcgca
     3721 gcacgaggtc gtagaatgac ggcctcatag acgctggggg agccctttgg ggctgagagt
     3781 ccgcggagcc ggacaaccct ttgaacctga tctggattat gccagcgaag ggaagcggga
     3841 cgcagtgacc atcaaaccgc acccatgctg agtgcggttt ttttgtttgt acgggaagtg
     3901 ggagcgcgcc cggcaacaac gggacacacg cggacgtcaa cggtctccaa cgaatgaggt
     3961 gacgatgaac acgcacgatc aggacacggg cactggctac gcggcgattg ccgaggaggt
     4021 gacgggcagg gggttgacgc tcacgccgtt tcccgcgtcg cgcaagatcg cggtggaagg
     4081 gacgcgcgtc agcgtggcga tgcgggagat ccaggtatct cccacccggc aggccaagga
     4141 cggatcggtg gagatcaacg agccggtggt agtgtacgac acgtcgggcc cgtataccga
     4201 ccacgccgcg acgatcgaca ttcggcgcgg gattcacccg ctgcgtcacg cgtggatcat
     4261 ggaacgggga gacgtcgagg aattgccgcg cgggtcgtcc gcgtacggcc gccggcgcga
     4321 agaggacccg cgtctggacc cgattcgttt cccgaacctg cgcaagccgc tgcgcgcccg
     4381 atccgacggc aacgtcaccc agatgcacta cgcgcgcacg ggcctggtga cgccggaaat
     4441 ggagtacatt gcgatccgtg aaaatcagct ccgggagcgg cttctggacg cggcgagcgg
     4501 cgcggctcct cgcggccacc tggcgcacca acatcccggc caggggtggg gcgcgagcat
     4561 ccccaaactc atcacgccgg agttcgtccg cgacgaggtt gcgcgcggcc gggccatcat
     4621 tccggccaac atcaaccatc ccgagtccga gcccatgatc atcggccgca actttctggt
     4681 caagatcaac gcgaacatcg ggaactcggc ggtggcctcg tcgatcgaag acgaggtcga
     4741 aaaaatgatc tgggccatcc gctggggctc ggacacggtg atggatctgt ccacgggcaa
     4801 gaacatccac gagacccgcg aatggatcat ccgcaacgcg ccggtgccga tcgggaccgt
     4861 gcccatctat caggcgctcg aaaaagtcga ggggcgggtc gaggaactca cctgggacct
     4921 ctttcgcgac acgttgatcg agcaggccga acagggcgtg gactacttca cgatccacgc
     4981 gggggtgctg ctgcgctacg tgccgttgac cgcgtcgcgc gccgccggca ttgtctcgcg
     5041 cggcggctcg attctggcca aatggtgtct ggcgcaccac caggagaact tcctctacac
     5101 gcacttcgag gagatctgcg agatcatgaa ggcgtacgac gtctcgttct ccctgggcga
     5161 tggcctgcgt cccggcgcga tcgccgacgc caacgacgag gcccagttcg gggagttgga
     5221 gacgctgggc gaactcaccc ggatcgcgtg gaaacacgac gtgcaggtga tgatcgaagg
     5281 ccccggccac gtgcccatgc acctgatcca cgagaacatg accaagcaac tcgaagtctg
     5341 ccacgaggcc ccgttctaca ccctggggcc tctgaccacg gacatcgcgc cggggtatga
     5401 tcacctcacc tcgggcatcg gcgcggccat gatcggctgg tacgggtgcg ccatgctctg
     5461 ctacgtcacc cccaaggagc acctgggact gccggacaag gacgacgtta aacaaggggt
     5521 gatcgcgtac aagatcgcgg cgcacgccgc ggacttggcc aagggccacc cgggggccca
     5581 acggcgcgac aacgcgttgt ccaaggcgcg gtttgagttt cggtgggagg atcagttcaa
     5641 cctctcgctc gaccccgaaa ccgcgcgggc ctaccacgac gaaaccctgc cgaccgaggg
     5701 tgccaaagtg gcgcacttct gttcgatgtg cggacccaag ttctgttcca tgaagatcac
     5761 gcaggacgtg agggattacg cggcgcaaaa ggcgctggac gacgccaagg cgatcgaggc
     5821 cggtatgacc gagaagtccg aggaattcaa aaaggcgggc ctggaaattt accggtagca
     5881 ggaccaccgg tgtcggccga cggtgtaggg gcacggcatg ccgtgcccct acgatgaaca
     5941 ggcgacggcg tccggcacga cgaacaagga tatggacatg aaccagacac gatccatcgc
     6001 acccacccga gagcgggaca ttacccgggc gatcgcccgc gaatactttc gccagttcga
     6061 cgcgctggtc gagtcggacg cgctgatcat cggctcgggt ccatcgggct tggtctgcgg
     6121 ccgagacctc gcgctggcgg ggttcaaagt cgtcatcgtc gagcagatga atcatctcgg
     6181 cgggggcttc tggaacggcg gctacctgat gagcaaggcc accatcgccg cgccggccca
     6241 gaccattctc gaagaagtcg gggtgccgtg ccgggaggtg gggcccgaga tgtacgtcgc
     6301 ggatcccccg cacgcgaccg cgaagttgat cggcgcggcg tacgacgcgg gcgtgaaggt
     6361 gatcaacttc accaaggtgg tggacctggt ataccgcgcg aatcgcctgg agggcgtggt
     6421 cgtgaattgg accccgctcg aaatggttgg tcacgacgtc gcccacgtcg atcccatcgc
     6481 gctggaatcg cgcgtggtcg tggacgccac gggtcacgac gcggtggcgg tccagatgct
     6541 cgcgaagcgc gggctgcacc agcccgtgcc cggcaacggc gcgatgtggg tggatcagtc
     6601 cgaggaaatc gtgatcgaca agaccggcga ggtgtacccg aatctcttcc tggtcggcct
     6661 ctccgtggcc gcggtgtacg gcacgccccg catgggcccc gcgttcggct ccatgctgct
     6721 ctcgggccgc aagggcgcgg gacagattgc ggcgaagctg ctggccgcaa gcgccgcggc
     6781 ccgctgacca ctgttccggc caactccgcc caaccgagcg atcccgctgg ggagccggat
     6841 gccgccgcct caacacagag gttttacggc gtcggcacga ggcgagagta aggcctgcgc
     6901 gaactgcgga cgcgtaccct aatgagggca ggtaatgtcc gctcgataca tcgagatggt
     6961 tgctggtggt gacacattcc cactggagtc aatcgccaca aactcaaagc tgtctgtcct
     7021 gaaaatgtcg tgacagacat tcggcgtata gatgacaaac ggcgcgccag agacagttcc
     7081 cctccccggt cccgttgttt cgtctacggt ggacgaagac gtcgccattg cctgggtctg
     7141 agcaccggtg atcggatcaa tgaattggta cgtgattcct tggaccatac tcatggtctt
     7201 tgtcgtatct tgatgatcgg ggaatcgtgt aatccgaaac gtcacgggac cgccatccgg
     7261 tgcagatcct gtg
//
LOCUS       DBNGTT010000064         5582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_652819, whole genome shotgun sequence.
ACCESSION   DBNGTT010000064 DBNGTT010000000
VERSION     DBNGTT010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_652819"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..2258
                     /locus_tag="AC16BC_01465"
     CDS             18..2258
                     /locus_tag="AC16BC_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HIR3240309.1"
                     /translation="MKISLRLIVSLVVVLTAAVVVFTYSQARREYDRSANDLLRRAQL
                     MGESLQETVATALANHQTRSLRRIVDRFANRERLVGIMVFDPTGTLIAATAALSTPPS
                     VTDTQSVVKTERESAGLLDRNGRLLHVYAAPLIEDEQVVGVLAVVHDAQYIRDRVTQI
                     WTDSFFRLLTYAFAIALTTLLVVRWSIHGPIARMTEWMKQLRLGQVPAGKPPVPEAFF
                     KPLASEAARMVSSLTEARASAEHEARLRLISESLWTAERLKEFVRTKLEGRAFVLVSN
                     REPYIHTRRGKVVEWVVPASGLVTALEPVMRASEGLWVAHGSGDADSLTVDEHDRVRV
                     PPDEPAYTLKRVWLTKDEEQGYYYGFSNEGLWPLCHIAHTRPIFRSDDWAAYQAVNAK
                     FAEAVLREIEESPEPVVLIQDYHFALLPRLIKTARPDARVGLFWHIPWPNPEAMAICP
                     WQREILNGMLGADLIGFHIQFHCNNFLDTVDRALECRIDRERFAVTREQQTTLVRPYP
                     ISVAPWPDAPTGSDTREALLKEFGLPPAVRVGVGVDRLDYTKGLLERFRGIERFFEKY
                     PDFRERFTFVELGAPSRTHIPRYHDLVSELDAEADRIAWRFRTKTWTPLLFLKGHHTH
                     REILRWYRVADVCLVTSLHDGMNLVAKEYVSARDDASGSLILSPFTGAARELVTALIV
                     NPYDTEQLADAIAYALGMPADEQRRRMSAMREQIRSSNIYRWAANLVADVAEVRVATP
                     EPFGGP"
     gene            2258..3094
                     /gene="otsB"
                     /locus_tag="AC16BC_01470"
     CDS             2258..3094
                     /gene="otsB"
                     /locus_tag="AC16BC_01470"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR3240310.1"
                     /translation="MAVHPSTDGAPRERAERRPIAVSPAALAKRLTKRSVLLCLDYDG
                     TLVPIAGRPELATFDDSGRRLLRGLSRRMPVAIVSGRARAALRSLIGVPSLYYVGNHG
                     FEISGPRVRLRAPLPPDWPRELERLLTTIESDAPPGVLIERKGPTASVHYRLVAPEDR
                     RRWLPALRARLDVLAGDGHLRVLRGKTVLELRPPGHWHKGAAVRWLTERRCFTGRIPV
                     YLGDDATDEDAFRAIRHDGIGVLVGPSRRTAARYRLPGPAHVRRFIEQWLGESGSDER
                     PR"
     gene            3331..3765
                     /locus_tag="AC16BC_01475"
     CDS             3331..3765
                     /locus_tag="AC16BC_01475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3240311.1"
                     /translation="MEQTSAGGIVFRRVDGRVEVALIARTSPANRLIWALPKGWVEPG
                     ETIPDAALREIREETGLTGRLLDALGQIAYSFYSPRDHARIHKTVHFYLVEYVSGDTA
                     DHDHEVDEARWWLLDEALGRLTYPSERGIMEKAKERLASPSA"
     gene            3762..4625
                     /locus_tag="AC16BC_01480"
     CDS             3762..4625
                     /locus_tag="AC16BC_01480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3240312.1"
                     /translation="MNISAVPELPLTFTAVLRGRPVTYRLVVSPRARAWRVSIHPHTG
                     LSVVVPAGARMDISALLDAHAAWIHRHLDRLARDPRPARTPLAHGSLLPYRGTALRLD
                     VTRSRSDAPHYDPVIGMLRVAGGSDRRLLTEVEAWYRRQADGVFLDRLHAVNAVLGYR
                     FGRVSIRDQKTRWGSCSPTGNLSFNWRLLMAPPSVLDSVVAHELTHLADRSHAPSFWN
                     RLAAIDPDYDAHRRWLARYGSWLALGCDPAPPVSPPQHAGRLPREETPRSALDGGGGS
                     PSPRGGFSTTG"
     gene            complement(4609..4917)
                     /locus_tag="AC16BC_01485"
     CDS             complement(4609..4917)
                     /locus_tag="AC16BC_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240313.1"
                     /translation="MKSRWFHTASLAGLLAAVAVSANAQDFSLKAEGVNPLYPAPSSH
                     VALVGAAAPAEADHALNALDKNPLYAGVGRAESVMSAGGAAPRISDPSLKAADVNPLY
                     "
     gene            complement(4985..>5582)
                     /locus_tag="AC16BC_01490"
     CDS             complement(4985..>5582)
                     /locus_tag="AC16BC_01490"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3240314.1"
                     /translation="SRIRTIPEYVDLFKQAFPGEKDPITYDNIAKAIAAFERTLLTPS
                     RFDDFLKGKADALTTVEKQGLSVVIQKGCIACHNGVGAGGGLYQKFGIRDRYEYSDDH
                     GRYNVTKNEQDKYFFKVPMWRNVTRTAPYFHDGSIWDLKEAIRIMGRLQLGANLSDNE
                     VDQIAAFLHALEGRIPDEAMRLPVLPSSTLATPKPVFK"
BASE COUNT          902 a         1905 c         1796 g          979 t
ORIGIN      
        1 gcggggagga tgggcacatg aagatttctt tgcgactgat cgtgtcgctg gtcgtggtgt
       61 tgaccgcggc ggtcgtggtg tttacgtaca gccaggcccg acgggaatac gaccgctccg
      121 cgaatgattt gctgcggcgc gcgcaactca tgggggagag cctccaggaa acggtggcga
      181 cggccctggc gaatcaccag acccgttcgc tccgtcggat cgtcgaccga ttcgccaacc
      241 gggagcggtt ggtgggcatt atggtgttcg acccaaccgg tacgttgatc gcggccaccg
      301 ccgcgctgtc cacgcctccg tcagtcaccg acacccaatc ggtggtgaaa accgaacgcg
      361 agagcgccgg gttactcgac cgcaatggtc ggctgctcca cgtatacgcg gcgccgctga
      421 tcgaggacga acaggtagtc ggcgtgctcg ccgtggtcca cgacgcccaa tacatccgag
      481 accgcgtgac gcagatctgg acggacagtt ttttccgcct gttgacctat gcgttcgcca
      541 tcgccctcac gacgctgctc gtggtccgat ggagcatcca cggacccatc gcgcggatga
      601 ccgaatggat gaaacaactc cgcctgggtc aggtcccggc cgggaagccc ccggtgccgg
      661 aagccttctt caaaccgttg gcgtctgagg cggcgcgaat ggtctccagc ctgaccgagg
      721 cccgcgcttc ggccgagcac gaggcacgcc tccggctgat ttccgaatcg ctctggaccg
      781 cggaacggct caaggaattc gtgcgcacca aactcgaagg acgggcgttc gtcctggtgt
      841 ccaaccgcga gccctatatc cacacgcgcc ggggcaaggt cgtcgagtgg gtggtgccgg
      901 ccagcggatt ggtcacggcc ttggagcccg tcatgcgggc atccgaagga ttgtgggtcg
      961 cccacggaag cggcgacgca gactccctca ccgtggacga gcacgaccgc gtgcgcgttc
     1021 ctcccgacga acccgcctac acgctcaagc gcgtgtggct caccaaggac gaagagcaag
     1081 gctactatta cggattctcc aacgaaggac tctggcccct gtgccacatc gcccacacgc
     1141 ggccgatctt tcggagcgac gattgggccg cgtaccaggc ggtcaacgcc aagttcgccg
     1201 aggcggtgct gcgcgagatc gaagagagcc cggagcccgt ggtcctgatc caggactacc
     1261 atttcgccct gctgccgcgc ctgatcaaga ccgcgcgacc cgacgcgcgg gtcggcctgt
     1321 tttggcacat cccgtggccg aatcccgagg ccatggcgat ctgcccgtgg caacgcgaga
     1381 tcctgaacgg tatgctgggg gcggatttga tcggctttca tatccaattt cactgcaaca
     1441 actttctcga cacggttgac cgcgccctgg agtgccggat cgaccgcgag cgattcgccg
     1501 tcacccgcga gcaacagacc acgctggtcc gcccgtaccc gatcagcgtg gccccgtggc
     1561 ccgatgcgcc gaccggctct gacacccgcg aggcattgct caaggaattc ggactgccgc
     1621 ccgcggtgag ggtgggcgtg ggggtggacc gtctggatta caccaagggc ctcttggagc
     1681 ggttccgcgg catcgagcga ttctttgaga aatatcccga cttccgcgag cgcttcacgt
     1741 tcgtggaact gggcgccccg agccgcacgc atatcccccg gtatcacgac ctggtgagcg
     1801 aactcgacgc ggaggcggat cgcatcgcgt ggcgctttcg caccaagacc tggacccccc
     1861 tgttgtttct caagggccat cacacccacc gcgagatcct gcgatggtac cgcgtcgcgg
     1921 acgtctgcct ggtgacctcg ctgcacgacg gcatgaacct cgtcgccaag gagtacgtca
     1981 gcgctcggga cgatgcctcg gggtccctga tcctgagtcc gtttaccggg gccgcacggg
     2041 aactcgtcac tgcgttgatc gtcaacccct acgacacgga gcaactcgcc gacgcgatcg
     2101 cgtacgcgct gggcatgccg gcggacgagc agcgccggcg gatgtctgcg atgcgcgagc
     2161 agatccgttc atccaacatc tatcggtggg ccgcaaattt ggtcgcggac gtggccgagg
     2221 tgcgcgtcgc cactcccgaa ccgttcggcg gaccgtgatg gcggtccatc cctccaccga
     2281 cggcgctccc cgtgaacggg ccgaacgccg gcccatcgcc gtcagtcccg cggcgttggc
     2341 gaaacggttg acgaagcgct cggtcctcct gtgcctggac tacgatggaa ccctggttcc
     2401 aatcgccggc cggccggagc tggcaacctt cgatgattcc ggccgtcgcc tcttacgcgg
     2461 actctctcgg cgaatgccgg tcgcgatcgt cagtggccgc gcacgcgcgg cgttgcgcag
     2521 cctcatcggc gtgccctcgc tctactacgt gggtaaccat ggattcgaga tctcggggcc
     2581 ccgcgtccgg ctccgcgcgc cgcttccgcc cgactggccg cgcgagttgg agcgcctgct
     2641 gaccacgatc gagtccgacg cgccgccggg cgtcctgatc gagcggaaag gccccaccgc
     2701 cagcgtgcac tatcgcctgg tagcgccgga ggaccgccgt cgctggctgc cggctctgcg
     2761 cgcgcggctc gacgtcctgg cgggcgatgg ccacttgcgt gtcctgcggg ggaagacggt
     2821 ccttgaactg cgcccgccag ggcattggca caaaggcgcg gcggtgcgtt ggttgaccga
     2881 acgccggtgc ttcacgggac gtatcccggt gtacctgggc gacgacgcga ccgacgagga
     2941 cgcttttcgc gccattcggc acgacgggat cggcgtcttg gtggggccgt cgcgccgcac
     3001 cgccgctcgc taccggttgc ccggccccgc gcacgttcgc cgtttcatcg agcaatggct
     3061 cggcgagagc ggcagcgacg agcgcccgcg atagtcgcgt cgaccgcggt tcaggagatc
     3121 ccgtccgtca cccggatcgt ccacgtcgga tggggggcgc tgcacgagac catcgctcga
     3181 cggtatgcga tcggtctggc catggtcccg cggggccgaa cgcgctctcc cgcgccgtcg
     3241 aggctttctt cgccacaata gcgaatcgag ttgacagctc tcgggttcga tccctacact
     3301 aacgccgccg tgggaaaccc gaccagaccc ttggagcaaa cctccgcggg cggcattgtg
     3361 ttccgccgcg tggacggtcg cgtggaggtc gccctcatcg cgcgcaccag ccccgccaac
     3421 cggctgatct gggccttgcc caaaggctgg gtcgagcccg gcgaaaccat tcccgacgcc
     3481 gcgctccgcg agatccggga ggaaaccggc ctgaccggcc gtctcctgga tgcgttgggc
     3541 cagatcgcct actcgtttta ttcgccccgc gatcacgccc gaatccataa gaccgtgcac
     3601 ttttatctgg tcgaatacgt gagcggcgat accgcggatc acgaccacga agtggatgaa
     3661 gcgcgctggt ggctgttgga tgaggcgctc ggccgcctga cgtatccgag cgaacgcggg
     3721 atcatggaaa aggccaagga acggctcgcc tccccgagcg cgtgaacatc tcggccgtcc
     3781 ccgaacttcc cttgacgttt accgccgtgc tgcgggggcg accggtcacg taccggctcg
     3841 tggtcagtcc gcgcgcccgc gcttggcgcg tgtcgattca cccgcacacc ggcctctcgg
     3901 tcgtggtccc ggcgggcgcc cggatggata tctccgcgct gctggacgcc cacgccgcct
     3961 ggatccaccg gcatctggac cgcctcgccc gcgacccgcg gcccgctcga acaccgctgg
     4021 cgcacggcag cctgttgccg taccggggaa cggccttgcg attggacgtg acccgcagtc
     4081 ggtccgacgc gccgcactac gacccggtga tcgggatgct tcgagtcgcc ggcggaagcg
     4141 accgccggct gctgaccgag gtggaagcgt ggtaccggcg ccaggccgac ggtgtgttcc
     4201 tcgatcggct tcacgcggtg aatgccgtct tgggctatcg gttcggccgg gtgtcgatcc
     4261 gggaccagaa gacgcgatgg ggcagttgct cgccgaccgg caatctctcg ttcaattggc
     4321 ggttgctgat ggccccgccc tccgtgttgg actccgtggt cgcccacgag ctcacgcacc
     4381 tggcggatcg cagccacgcc ccgagcttct ggaaccgcct cgccgcgatc gaccccgact
     4441 acgacgcgca ccggcgctgg cttgcgcgat acggttcctg gctggctctg gggtgcgatc
     4501 cggcgcctcc ggtttctccc ccacaacacg cggggcgcct cccccgcgag gagacgcccc
     4561 gaagtgcgct tgatggcgga ggcggatcgc cttcgccgcg tggcgggttc agtacaacgg
     4621 gttgacgtcc gcggccttca gcgagggatc gctgatccgc ggggccgcgc cgccggcgga
     4681 catcaccgac tcggcccggc cgaccccggc gtagagcggg ttcttgtcca gcgcattcag
     4741 cgcatgatcc gcttccgccg gcgcggcggc gccgaccaac gcgacgtgag acgacggcgc
     4801 gggatacagc ggattgaccc cctccgcctt gagtgagaaa tcttgtgcgt tcgcgctgac
     4861 ggccaccgcc gcgagcaacc ccgccaggga cgccgtgtgg aaccaacgac tcttcattgc
     4921 ctgctccttt ctcgttcgtc aatctcctcg caacgccgaa cggcggcggg agaccgacac
     4981 tcggttactt aaacactggc ttcggtgtgg ccagcgtcga cgagggaagc accggcaatc
     5041 gcatcgcctc atccgggatt cggccttcca gcgcgtgcag gaacgccgca atttgatcaa
     5101 cctcgttgtc gctcaggttc gccccgagct ggagacgccc catgatccgg atcgcttcct
     5161 tgagatccca gatcgaccca tcgtggaaat agggcgcggt cctggtcacg ttccgccaca
     5221 tcgggacttt gaaaaagtac ttgtcctgct cgttcttggt aacgttgtac cggccgtgat
     5281 cgtcgctgta ttcgtatcga tcgcgaatcc cgaatttctg gtacaacccg cccccggcgc
     5341 ccacgccgtt gtgacacgcg atacacccct tctggatcac caccgacaac ccctgcttct
     5401 cgaccgtggt caacgcgtcg gctttgccct tgagaaagtc gtcgaaccgc gagggcgtca
     5461 gcagggtgcg ctcgaacgcc gcgatggctt tggcgatgtt atcgtaggtg atcggatcct
     5521 tttccccggg aaacgcttgc ttgaagagat cgacgtattc gggaatcgtc cgaatgcgcg
     5581 ac
//
LOCUS       DBNGTT010000065         4124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2185872, whole genome shotgun sequence.
ACCESSION   DBNGTT010000065 DBNGTT010000000
VERSION     DBNGTT010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2185872"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC16BC_01495"
     CDS             complement(<1..344)
                     /locus_tag="AC16BC_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012185363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HIR3240315.1"
                     /translation="MITVDGSYGEGGGQIVRTALALSCVTGKPVRINDVRKNRSKPGL
                     QPQHLTGVLACALISRAKVEGAVLGSDRLMFSPGRLCGGEYTFDVEQTAGKGSAGSIA
                     LILQAILLLLSRA"
     gene            506..2086
                     /locus_tag="AC16BC_01500"
     CDS             506..2086
                     /locus_tag="AC16BC_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR3240316.1"
                     /translation="MTDALIQALQDPALYDHPVDRFEVIETHISWVLLTGPYAYKLKK
                     PVDFGFVNFTTLERRKFFCGEELRLNQRLAPAWYLDVVPITGTVDGPRLRGAGPAIEY
                     AVKMKQFPQDARLDLVLVRGALTPERLDAEVSAIAEFHGRAAVAAVDSPFGTPERIAN
                     RVQENLATIREHLADPGDLPLVERLDSRARDEHAALARTFAARKRDGFIRECHGDLHL
                     GNMAWVDGRPLIFDCIEFNDDLRWIDVMSEFAFLVMDLDHRDAAALARRALNAYLEAT
                     GDYAGLRVLPYYYAYRSLVRAKVACLRLRQGDPDPAARAATRRELRGYLDLAAGPSTS
                     DRPVLIMTHGVSGSGKTHVAQAVVEALGAVRIRSDVERKRLAGLSPRSRAGSEPGGGI
                     YSRELTERTYARLGDLAGMSLDAGYPVVVDATFLKRAQRDGLRAVARSHAAPCVILAA
                     QAPESVLRERIVARAQHGRDASDADLAVLDGQLRSMDPLATDEQGIAVTVDTARALDP
                     AWLVEAIRARGAGAATGL"
     gene            complement(2097..2801)
                     /locus_tag="AC16BC_01505"
     CDS             complement(2097..2801)
                     /locus_tag="AC16BC_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3240317.1"
                     /translation="MPTAANSVCRICPYREQSVFCELPEHRLDEFCRLKLVSRYQKGQ
                     HIFYEGEPNLGLSILCSGKVKLDRTSNSGKTQILNILGPCGLLGEKDLFLSDRHTVSA
                     EALEDSVVGFVRKDDFLAFLTDNPTVALRVIRRLAEQLAKIEERSFALTALDVRQRLA
                     ALLLDLADQHGKTLSGECLIDVALTRENLAEMIGATPETVIRVLSAFRKDGVIKDTAK
                     QLVVLRPDELQRIAAS"
     gene            3074..3337
                     /locus_tag="AC16BC_01510"
     CDS             3074..3337
                     /locus_tag="AC16BC_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240318.1"
                     /translation="MPVPAHLEEAAARLSEAVWRIDQAKAKPLSEDTQREWLSALTDF
                     SLALSDIQRFNNESIHEKLQGLDGRVERLTRAGSSAEPPVDIE"
     gene            complement(3387..3902)
                     /locus_tag="AC16BC_01515"
     CDS             complement(3387..3902)
                     /locus_tag="AC16BC_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HIR3240319.1"
                     /translation="MTSKGRTQAAKPRAPRKGLRVVLSQDSRQDTYRLRGKLPEPTVC
                     PECHAVFHKGRWTWGKRPEDAHEHTCPACHRVRDKFPAGWITISGLYLAAHRAELLGT
                     ARNEETAQRADHPLSRIMAIEEHPDRVTITTTDLHLPRRIGEALRRAHDGELTIEHEK
                     QSYLVRVAWIR"
     gene            complement(3914..>4124)
                     /locus_tag="AC16BC_01520"
     CDS             complement(3914..>4124)
                     /locus_tag="AC16BC_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240320.1"
                     /translation="AMGVAPADVLRRIQDMVDQVVCLETPDVMFAVGEFYREFPSVTD
                     EEVVEILKQRRSPTDTGARTKHTHP"
BASE COUNT          673 a         1266 c         1439 g          746 t
ORIGIN      
        1 gcgcgcgaga ggagaagcag gatggcctgc aggatcaggg cgatcgagcc cgccgaaccc
       61 ttgcccgcgg tctgttccac gtcgaacgtg tactcgccgc cgcagagccg gcccggggaa
      121 aacatgaggc ggtcggatcc caagacggcg ccttcgacct tggcgcggct gatcagcgcg
      181 cacgcgagga cgccggtcag gtgctggggt tggaggccgg gcttgctccg gtttttgcgg
      241 acgtcgttga tccggaccgg cttgcccgtc acgcaagaga gcgcgagcgc ggttcgcacg
      301 atctggcctc caccttcgcc gtaggaaccg tcgacggtga tcatgtgatc gctggtcaaa
      361 tccccccttt gaaaaatggg ggcaaggggg gatttatccg gcgagtaacc gggcaagcgt
      421 gtacggagtg ttcagcgaac acggtggctc attgtaacgc aatcgtcgat tcggtatgct
      481 gcgggctgtc gtcgcgagcc acggcatgac cgacgccctc atccaggcgc tccaagatcc
      541 cgcgctctac gaccatccgg tcgatcgatt cgaggtgatt gaaacgcaca tctcgtgggt
      601 gctgctgacc gggccctacg cgtacaagct caagaagccg gtggacttcg ggttcgtgaa
      661 tttcacgacc ttggaacggc gcaagttctt ctgcggggaa gagctgcgtc tcaatcagcg
      721 gctggcgccg gcgtggtatc tcgatgtggt gccgattacg ggtacggtcg atggtccgcg
      781 actgcgcgga gccgggcccg ccatcgagta cgcggtgaag atgaaacaat ttccgcagga
      841 cgcgcggctg gatctggtgc tggtgcgcgg ggcgctcacc cccgagcggc tcgacgccga
      901 ggtgtcggcc atcgcggagt ttcacggccg cgccgcggtc gcggccgtgg attcgccctt
      961 cggcacgccg gagcgcatcg ccaatcgggt tcaggaaaat ctcgccacca tccgcgagca
     1021 cctggccgat cccggcgatc ttccgctcgt tgagcggctg gacagtcggg ctcgagacga
     1081 acacgcggcc ttggcccgga cgttcgcggc gcgcaagcgc gacgggttca tccgcgagtg
     1141 ccacggcgac ctgcacctgg gcaacatggc gtgggtcgac gggcggccgc tgatcttcga
     1201 ttgcatcgag ttcaacgacg atttgcgttg gatcgacgtg atgagcgagt tcgcgttcct
     1261 ggtgatggat ctggaccacc gcgacgccgc ggccttggcg cggcgcgcgc tcaacgcgta
     1321 cctggaggcc accggcgatt acgcgggcct ccgcgtgttg ccgtattact acgcgtatcg
     1381 gtcgctggtc cgggccaagg tggcgtgtct gaggttacgg cagggcgatc cggacccggc
     1441 ggcgcgagcc gcgacccgcc gcgagcttcg cgggtatctg gatctggcgg ccggaccctc
     1501 gacgtcggat cggccggtgc tgatcatgac ccacggcgtc tccggctccg gaaagaccca
     1561 tgtggcgcag gccgtggtcg aggcgctcgg ggccgttcga atccgaagcg acgtggaacg
     1621 caaacgtctg gccggtttgt cgccgcggag ccgcgcgggc tccgagcccg gcggcgggat
     1681 ctattcgagg gaactgacgg agcggacgta cgccaggctc ggcgacctgg ccgggatgtc
     1741 gctggacgcg gggtatccgg tcgtcgtgga cgcgaccttc ctgaagcgcg cgcaacgaga
     1801 cggcctgcgc gccgtggccc ggtcgcacgc cgcgccgtgc gtcatcctcg cggcgcaggc
     1861 gcccgagtcc gtgctgcgcg agcggatcgt ggcgcgcgcg cagcacgggc gcgacgcgtc
     1921 ggacgcggac ttggccgtgc tcgacggcca gttgcggtcg atggatccgc tggccacgga
     1981 tgaacagggc atcgcggtga cggttgatac cgcgcgcgct ctcgatccgg cgtggttggt
     2041 cgaagcgatc cgcgcgcgcg gcgcgggtgc agcaacaggt ttgtgaataa accgggctac
     2101 gacgcggcga tgcgttggag ctcgtcggga cggagcacga cgagctgttt ggccgtatct
     2161 ttgatgacgc catccttgcg gaacgcgctc aagacgcgga tcacggtttc cggggtggcg
     2221 ccgatcattt ccgccaggtt ctcgcgggtg agcgcgacgt cgatcaggca ctccccggac
     2281 aacgttttgc cgtgttgatc cgcgagatcc aggagcagcg cggccaggcg ttgtctgacg
     2341 tccaacgcgg ttagggcgaa cgaacgctcc tcgattttcg cgagttgctc ggcgagtcgc
     2401 cggatcaccc gcagggccac ggtgggattg tcggtgagga aggcgagaaa atcatctttt
     2461 ctcacgaagc ccaccacgga gtcttcgagc gcctcggcgc tgaccgtgtg ccggtcggag
     2521 aggaacaggt ccttttcccc taggagcccg cacggaccca ggatgttgag aatctgggtc
     2581 ttgccggagt tcgacgttcg atcgagcttg accttgccgg aacagaggat cgagaggccc
     2641 aggttgggct cgccctcgta gaaaatgtgc tgacccttct gataccggct cacgagtttc
     2701 aggcgacaga actcgtcgag acggtgctcc ggcaattcgc agaagacgct ctgctcacgg
     2761 taggggcaaa tccggcaaac gctgttcgcg gcagtgggca tcatacgtcc tgccgctact
     2821 atgacggcga cggtcggcca aagtcaacgg gcggtcgtcc ctgcgatgaa cggcgcgcgc
     2881 tgcgcatgcc cgcgtttccg ccggcttcac aaggtttggg ggactcgacg tgctccgctc
     2941 gggtgtttac ggggcggaac ctcgattggt aaccctgaaa tacttcgttc gagtcattgg
     3001 gtttgccgtg gcagatcggc agaatggggc tgcgtgagga tgggtgatcg gtcgattgaa
     3061 aaaggaggag cgtatgcctg taccggcgca tttggaggaa gctgcggcgc ggctcagcga
     3121 ggccgtctgg cggatcgacc aagccaaggc caaaccgttg agcgaagaca cgcagcggga
     3181 gtggttatcc gcgctcacgg acttttctct ggcgttgtcg gacatccagc gctttaacaa
     3241 cgaatccatc catgagaaac tgcagggtct cgatggacgg gtcgagcggc tgacccgggc
     3301 gggatcctcg gcggaaccgc cggtggacat cgagtgacgc cgggtccccc cctcacgaac
     3361 ccgtagccgt cttcggccgc gcgcggttac cgaatccagg ccacgcgcac gaggtacgac
     3421 tgcttttcgt gttcgatcgt caattcgccg tcgtgcgcgc gacgaagcgc ttcgccgatg
     3481 cggcgcggca ggtgcaggtc cgtggtggtg atcgtcaccc ggtcgggatg ttcctcgatc
     3541 gccatgatgc gggacagggg atggtcggcc cgttgcgcgg tttcttcatt gcgcgccgtg
     3601 cccagcaact ccgcgcggtg ggcggccagg tacaggccgc tgatcgtaat ccagcccgcg
     3661 ggaaacttgt cgcgaacgcg atggcaggcc gggcacgtgt gctcgtgcgc gtcctccgga
     3721 cgtttccccc acgtccatcg gcctttgtgg aacaccgcgt ggcactcggg acacacggtc
     3781 ggctccggca gtttgccgcg gaggcggtag gtgtcctggc gggaatcctg agagagcact
     3841 acgcggaggc ccttgcgcgg ggccctgggc ttcgctgcct gcgtccgtcc tttgctggtc
     3901 atggctccaa tccttagggg tgagtatgct tggtccgcgc gccggtatcg gtcggcgagc
     3961 ggcgctgttt gaggatctcg acgacctcct cgtcggtgac cgatgggaat tcccgataaa
     4021 actcgcccac cgcgaacatc acgtcagggg tttccaaaca cacgacctga tccaccatat
     4081 cctggatgcg gcgcaacacg tcggccggcg ccacgcccat ggcc
//
LOCUS       DBNGTT010000066         1560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2313730, whole genome shotgun sequence.
ACCESSION   DBNGTT010000066 DBNGTT010000000
VERSION     DBNGTT010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2313730"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="AC16BC_01525"
     CDS             complement(<1..608)
                     /locus_tag="AC16BC_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3240321.1"
                     /translation="MSTISLKTLTPEQMAEFVAAQGWPRYRTRQLLAWVYQRGVSRFE
                     HMTDIGRADRARLEQLAVIPEPRIVDVRRDPEDGTEKLLFRLEDGQTVESVLIPDEDR
                     LTLCLSTQVGCTLDCGFCLTGTMGLTRNLRGHEIVDQVLGARAHAGPERQITNLVFMG
                     MGEPLANLPAVCDAIERLTADWGLGFSPRRITVSTAGLVPQMA"
     gene            730..>1560
                     /locus_tag="AC16BC_01530"
     CDS             730..>1560
                     /locus_tag="AC16BC_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3240322.1"
                     /translation="MVTQPLRHAFNLFIGLLVGAMAAGCGQQAPGADESTGGTSGSDL
                     TPPTIVAKFPDDRNSNIPIDVKIALTFSEPIAHLRTFQYEPYIVLYESLQGSWSVVQR
                     DFSQWDDRSNTLTVIPKTLLRPDTQFMVQVSDGIIDQAGNRLDLGNGTHSITWRFSTG
                     STFDDQKPTWQAKKTVRVAADRYDTLTVCWWNDDPPQPFVGCDAAALATAPPAAIDPP
                     SNESGGLIYTVQYKRRVDAGFSSMPSGIGGKSVKLTGLVASTEYQIRVVVSDQSANQA
                     D"
BASE COUNT          307 a          494 c          489 g          270 t
ORIGIN      
        1 gccatctggg ggaccagtcc cgcggtcgac accgtgatcc tccgcggcga gaatcccagg
       61 ccccagtccg cggtcaggcg ctcaatggcg tcacacaccg cgggcaggtt ggccaacggt
      121 tcacccatgc ccataaatac gaggttggtg atctgccgct ccgggcccgc gtgcgcccgc
      181 gcgcccaaga cctgatccac gatttcgtgg ccgcgaaggt tgcgcgtgag ccccatggtg
      241 ccggtcaggc agaacccgca gtccaacgtg cagccgacct gggtggacag gcagagtgtg
      301 agtcggtcct cgtccgggat gagcaccgat tcaacggtct gaccgtcctc aagacggaag
      361 agcaatttct cggtgccgtc ctctgggtcg cgtcgcacgt cgacgatccg gggctcgggg
      421 atcaccgcga gctgttccag gcgggcgcgg tcggcgcgtc cgatgtccgt catgtgctca
      481 aatcgcgaga cgccgcgttg atacacccac gccagcaatt gccgggttcg gtaccgtggc
      541 cagccctgcg ccgcgacgaa ctcggccatc tgttcggggg tcagggtctt gagggagatc
      601 gtgctcatga ttgaactgat tgatttataa cacacttctc agccgaagtg acagtgctgg
      661 gggtgaaaac gccgggaaat aaaaccgggg aggacgcggt tttgataacg ggactgattt
      721 gccgcacaaa tggtgacgca accgttacgg cacgccttca atctgttcat cggacttcta
      781 gtcggcgcga tggccgccgg gtgcgggcag caagcgcccg gcgccgacga atccaccggg
      841 ggcacctcgg ggagcgatct cactcctccc accatcgtcg ccaaatttcc cgacgatcgg
      901 aactcgaaca taccaattga cgtcaaaatc gcgctcacgt tcagcgagcc gatcgcccac
      961 ctcagaacgt tccaatacga gccgtacatt gtgctgtacg aatcgctcca aggctcgtgg
     1021 tcggtggtcc agcgggattt ctcacaatgg gatgatcgaa gcaacacgct gaccgtgatt
     1081 cccaagaccc tcctcaggcc cgacacccag tttatggtgc aagtcagcga cgggatcatc
     1141 gaccaggcgg ggaaccggct ggatttgggg aacgggaccc actccatcac gtggcggttc
     1201 tccaccggca gcacgttcga cgaccaaaag ccgacgtggc aggccaagaa gaccgtgcgg
     1261 gtggcggcgg atcggtacga cacgttgacc gtgtgttggt ggaacgacga ccccccgcag
     1321 ccgttcgtcg ggtgtgacgc cgccgcgctg gcgacggcgc cgcccgcggc catcgacccg
     1381 ccgagcaacg agtcgggcgg attgatctat accgtccagt acaaacgccg agttgacgcg
     1441 gggttttcct ccatgccgtc cggtatcggc ggaaaaagcg tcaaactcac cggcctggtg
     1501 gcgagcaccg aatatcagat ccgcgtggtc gtcagcgatc agtccgccaa ccaggcggac
//
LOCUS       DBNGTT010000067         7672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_781112, whole genome shotgun sequence.
ACCESSION   DBNGTT010000067 DBNGTT010000000
VERSION     DBNGTT010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_781112"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="AC16BC_01535"
     CDS             <1..302
                     /locus_tag="AC16BC_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240323.1"
                     /translation="FQGGGFSLTSPSRCDTCAPTSIDLEWGPATDSGGSGLKGYWIYV
                     DGIQAVFTSDITATPTVRFTFRSTGVIDTTREHLYQVQAIDNAGNRSVLYGNLRT"
     gene            402..2363
                     /locus_tag="AC16BC_01540"
     CDS             402..2363
                     /locus_tag="AC16BC_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240324.1"
                     /translation="MSPIPPPSLISRTGRRVFVVQRRLLTQVLRHAALVVIAGAWSVA
                     GAQDDHEHHARLLKQSVVVFKASAFSILETGGAVPPHLEILFDVPSRGNEIHAHHLPG
                     WIRISVDRLAPVEHTYTDEEWHALADGALHLLLTAPSGPGAHTVAVTFKGIDRNGKPY
                     LRTKSFSLESPNPSEHYVLRFHAPPNDEPFMAIEPLGASLLRPSAGSPAPGELAYRIG
                     RFEAASGNHHNAASAFLAALQSGLPEPTQSNARLRLAESYGALGAPEEAESLLERLAT
                     EASDAAVRARAWVLIERIAYQDGRHDRVIQAHSRLGANAPPDLAGEAHALAGMSALAL
                     RAFSQAADFFRAVPKASADAPLALFGQAQALAGQGDAFTATTLFSKLTQTRSLFAASH
                     SDMSFHAHAALGFQFLVQGRYQEAVAELGRVPSDHALAHASLFGIGWGLQNAGEHVKA
                     IAVFDDLVTRAPDGPYAHEARLAMAASYAALRAPTRSVAAYRAALDALAKSTAALDRL
                     RTVVAGSAWDPLSDYELALPADARRLVRDTPTLALAVERYRWLVHVERDLRRTLAEFP
                     AFLTAPGANTLGGRLHTKDGSRLVAQGQELLTKIDVVERESRKALSALIIETADRERD
                     RLEGWSVAASLGIARNLRDDLGGEGLTLE"
     gene            2356..5214
                     /locus_tag="AC16BC_01545"
     CDS             2356..5214
                     /locus_tag="AC16BC_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240325.1"
                     /translation="MSNDTNRRRGASRLVGSGALLVLVACMSSVGAAAEAPFVIDSAK
                     RRAITQDVLSSQRDPRGRAAKLDALKAYERFVMEDLSGRSALRAEAMHTLGDLYTEIE
                     TAADRTPRAKTAPPPARSRAKSIAVYERLLAQYPKRAENDGALYQLARGYWESGRLDD
                     ATAALRRVLAEYPPGAYAPEAAFRLGLRAFAARDFKAAASLFERASRGQDPSLAEAAR
                     FHLGWTALNLQEYRKAADAFVAILDATTAKQRQGGVLSVADMPEAEAAFFNEVVKALL
                     LAFDYQGGPDEMRAYFTGSERRPYEETLYRTLGALYQEQDRTADAVAAYQAFLLAAPL
                     HPNAPKFQSAIADAYTRAKWQGPLIEARERLVEQYTPGTSWTRANPEAWTHAAQPLVK
                     DALYQLALYDHAQAQQSRRPAAWDKALARHDRFLALFPKDVEAPRMVWLRGEALFELG
                     RYADAADAYRRSAYDYPLHPQTRDAAYAAVAARDRLIPADGPVAADLADRLAGDSTQF
                     VGAYPDDTRNPDLLMKAADTALRAGRPESATDLARRLVKAYPTSRWTATANRLIGQGL
                     YDSGRFGDAEQAFRRALSGANAQQSATVAALAASSLYQAASRDRTAGRDTEAIAGFVR
                     LASDYPSTTLAPAALNEAAELHARSGKPADASALWQRLAEQYADSPEAPGAFRRLAAS
                     AETSGDLAAAIKWYGRLAERSEPGPRDELTWTIAALAEQARDWPRAEQTLSALAARSD
                     LPPDRAIEAGFRAGRAAANQRRTTATALTDAALARYRVWRGQPGPHETTQADVLAAQT
                     LVALGDQQAAACAAVRLKDPLEQSLAQKRAALNAALAVYAEAAEIKIAATTTEATHKI
                     GEVLNEFFHALLASDRPGNLTEEQLEQYNFLIEEQAAPFEERAVSAYETNVRRTQELG
                     LSDAWITESYERLADLRPARYRRSERLELLRRSMEAAP"
     gene            5211..5819
                     /locus_tag="AC16BC_01550"
     CDS             5211..5819
                     /locus_tag="AC16BC_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240326.1"
                     /translation="MTARPVTVFTMTVCAFLAACSGAAVRREPPATVTAAPDPAALFT
                     RALTAQESGNAERAQALWRQLIAAAPTQSAAHTNLGILLRQAGNTDEAIREYETAIRL
                     DPRDAWAYHNLGVAHRLRGAWQDAERAYRRAIELRPDQMDSHYNLGILYELYLNRPEE
                     ALTQYRAVLSHGGPDADTVAPWVRALERRLAPAPDAPTPETR"
     gene            5816..6175
                     /locus_tag="AC16BC_01555"
     CDS             5816..6175
                     /locus_tag="AC16BC_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240327.1"
                     /translation="MTLRSLIALCTTVAVVWSAGPALAASPASGSPAQSPAPQPPTAQ
                     APAPAKPKTKPRRVIIFGTTIVGDVLTPPIEKTVPWQRPADFRADTAPLTHDFTTELL
                     TPLDRNAILREAGENDH"
     gene            6222..6863
                     /locus_tag="AC16BC_01560"
     CDS             6222..6863
                     /locus_tag="AC16BC_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3240328.1"
                     /translation="MEIVQFLVANFKSGGIFMYGILAVMVVGTAIVIDRTLALVIRGR
                     VNAGLFWQEIDALLQQGKLADALQRADGSATPLGRVLASGLTAAQRGEGREGIDRRIE
                     EAVRSVLPQLEKRIHYLYTLSNVSTLVGLLGTVVGLITAFTAVSLADPAQKAALLANG
                     ISLALNNTAFGLMVAILLMLAYSYMQSRAARLGDEIDDYSLRLANRLSERAGR"
     gene            6863..7387
                     /locus_tag="AC16BC_01565"
     CDS             6863..7387
                     /locus_tag="AC16BC_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3240329.1"
                     /translation="MLPRLASRRRRPVADEAIHIVALWNLMIILIPFLLLSAVFSQTS
                     ILNLYLPPPQGAPAPGSAQLDRAPKPVVSILHDGYVLSDGPQIIAAIPLDEQGAYNTR
                     KLTELLVSIKRSIPAQQDIVILSEAETSYETVVHTMDAAREWVSLEGGQPLFPNISLG
                     RVGGATPAPLRSVP"
     gene            7384..>7672
                     /locus_tag="AC16BC_01570"
     CDS             7384..>7672
                     /locus_tag="AC16BC_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3240330.1"
                     /translation="MRREAGTRRHLEPPRLNLTAMVDVFTVLLVFLLKSYSAEGTLTA
                     AAQNLELPSSTSSIAPQPTITITISEDTLFVEDERVGALAEFAEQSGPDIPE"
BASE COUNT         1267 a         2794 c         2385 g         1226 t
ORIGIN      
        1 ttttccaggg aggaggattc tcgctcacga gcccctcccg gtgcgacacc tgcgcgccca
       61 cctccatcga cctcgagtgg ggaccggcga ccgacagcgg gggcagcggg ctcaagggct
      121 actggattta cgtcgacggc atccaggccg tcttcacgtc ggacatcacc gcgaccccca
      181 cggtccgctt cacgttccgg tcgacgggcg tcatcgatac gacccgagag cacctgtacc
      241 aagtccaggc gatcgacaac gcgggcaatc ggtcggtcct gtacggaaac ctgagaacgt
      301 gatgccgacg gatcggttgc gtttcccccg gtccttcgat tacgataccg cctgatcgct
      361 ggtttcatac gttgcccgcc cgcgggtggg tttggcccgc gatgtcgccg attccaccgc
      421 cttcgctcat cagccgcacc ggtcgccgcg tgttcgtcgt tcaacgccgg ctcctgacgc
      481 aggttcttcg acacgccgcg ctcgtggtaa ttgccggggc atggtccgtc gcaggagccc
      541 aggacgacca cgaacaccac gcccggctcc tcaaacaatc ggtggtggtc ttcaaggcgt
      601 cggccttttc gatcctagaa accgggggcg ccgttccgcc gcatctcgaa atcctgttcg
      661 acgtgccgtc gcgcgggaac gagatccacg cgcaccacct ccccggctgg atccggatct
      721 ccgtcgatcg gctcgcaccg gtcgaacata cgtataccga tgaggagtgg cacgcccttg
      781 ccgacggcgc cctgcacttg ctgttgaccg ccccgtccgg tcccggcgcc cacaccgtgg
      841 ccgtgacctt caaaggcatc gaccgcaacg gcaaacccta cctccggacc aaatcctttt
      901 cccttgaaag ccccaacccc tcggaacact acgtgctgcg atttcatgcc ccgcccaacg
      961 acgaaccctt catggccatc gaaccgctcg gggcatcgct cctgcgcccg tcggccggct
     1021 cgcccgcccc gggagaactc gcgtaccgga tcggccgctt cgaagcggcc tcgggaaacc
     1081 accacaacgc ggccagcgcg tttctcgccg cactccagag cgggttgccc gagcccaccc
     1141 agagcaacgc ccgtctccgg ttggccgaat cctacggcgc cttgggggcg ccggaagaag
     1201 cggagtcgct gctcgaacgc ttggccaccg aggcctcgga cgcggccgtc cgcgctcgcg
     1261 cctgggtcct catcgaacgg atcgcgtacc aggacggccg gcacgaccga gtgattcagg
     1321 cgcacagtcg cctcggcgcc aatgctccgc ccgacctggc gggagaggcc cacgcgctcg
     1381 ccggaatgag cgcgctcgct cttcgcgcgt tctcgcaagc cgcggatttt tttcgcgcgg
     1441 tgccgaaggc cagcgcggat gcgcccctcg cgctcttcgg gcaggcacaa gccctcgcgg
     1501 gtcaaggcga cgccttcacc gcgaccacgc tcttcagtaa actgactcag accaggtcgc
     1561 tttttgccgc gtcccactcg gacatgtcgt tccacgccca tgccgcgctc ggattccagt
     1621 ttctggtgca gggccggtat caggaagccg tcgcggaact cggccgagtg ccgtccgacc
     1681 atgcgctggc gcacgccagc ctcttcggca tcgggtgggg cctgcagaac gcgggagaac
     1741 acgtcaaggc catcgcggtg ttcgacgacc tcgtcacccg cgcgccggac ggtccgtatg
     1801 cccacgaagc gcggctggcc atggcggcct cctacgccgc gctgcgcgcg ccgacccgtt
     1861 cggtcgcggc ctaccgcgcg gcgctcgacg ctctcgccaa atccacggcc gcgctggacc
     1921 gcctgcgaac cgtcgtggcg ggttccgcct gggacccgct gtccgactat gagctcgcct
     1981 tgcccgcgga cgcgcgtcgg ctcgtccgcg acaccccgac ccttgcgctg gcggtggaac
     2041 ggtaccgctg gctcgttcac gtggagcgcg accttcgccg aactctcgcc gaatttcccg
     2101 cgttcctgac cgcccccggc gcgaacacgt tgggcggccg cctccacacc aaggacggct
     2161 cgcgcctggt cgcgcagggc caagaactcc tcaccaaaat cgacgtggtg gagcgcgaat
     2221 cgcgcaaagc gctgtccgcg ctgatcatcg aaaccgccga ccgcgaacgc gatcgcctcg
     2281 aggggtggtc ggtcgcggcc agcctgggga ttgcgcgcaa ccttcgcgac gacctgggcg
     2341 gcgagggcct gacgcttgag taacgatacg aaccgtcgcc gcggcgcaag ccgactggtc
     2401 ggatcgggcg cgctcctcgt gcttgtcgcg tgtatgtcgt ccgttggcgc cgcggcggag
     2461 gcgcccttcg tcattgattc ggccaaacgt cgcgccatca cgcaggatgt cctgtccagc
     2521 cagcgagatc cccgcgggcg agccgccaaa ctcgacgcgc tcaaggccta cgagcgtttc
     2581 gtcatggaag acctctcggg acgctccgcg ctccgggccg aggccatgca cacgctgggc
     2641 gatctctaca ccgaaatcga gaccgccgcc gaccggaccc ctcgcgccaa gacggccccg
     2701 ccgccggccc gatcccgggc aaaatcgatc gcggtctacg agcggctcct cgcgcaatac
     2761 ccgaaacgcg ccgaaaacga cggcgccttg tatcagctcg cgcgcgggta ttgggagagc
     2821 ggccgcctgg acgacgcgac ggccgcgttg cgccgggtgc tcgccgagta tccccccggc
     2881 gcgtacgcgc cggaagccgc gttcagactg gggcttcggg ccttcgccgc gcgggacttc
     2941 aaggccgcgg cgagcctgtt cgagcgggcg agccgaggcc aagatccgtc gctcgcggaa
     3001 gccgcgcggt ttcacctggg ctggaccgcg ctcaacctcc aggagtaccg aaaggccgcc
     3061 gacgcttttg tcgcgattct cgacgcgacc accgccaagc aacggcaggg cggcgtcctc
     3121 tcggtcgcgg atatgccgga agccgaagcc gcctttttta atgaggtcgt caaggcgctg
     3181 ctcttggcct tcgactacca gggaggaccc gacgagatgc gcgcgtattt caccggctcc
     3241 gagcggaggc cgtacgaaga aaccctctac cggacgctcg gcgcgttgta tcaagagcag
     3301 gaccgcaccg cggatgcggt ggcggcctac caggcctttc tcctcgccgc gcccctccat
     3361 ccgaacgctc cgaagtttca gtccgcgatc gcggatgcct acacccgcgc caagtggcag
     3421 gggccgctga tcgaggcgcg cgagcggttg gtcgagcagt acacgccggg cacgtcatgg
     3481 acgcgggcca atcccgaggc ctggacacac gcggcgcagc cgctcgtcaa ggacgccctg
     3541 tatcaactcg cgctctacga ccatgcacag gcccaacaga gccgacgccc ggcggcgtgg
     3601 gacaaggccc tggcgcgtca cgatcgcttc ctggccttgt tccccaagga cgtggaggcc
     3661 ccccgcatgg tctggctccg cggcgaagcc ttgttcgaat tgggtcgcta cgccgacgcc
     3721 gcggacgcct atcgacgcag cgcgtacgac taccccctcc acccccaaac ccgcgacgcg
     3781 gcatacgccg cagtcgcggc gcgggaccga ctcattcccg cggacggccc ggtcgccgcg
     3841 gaccttgcgg atcggttggc tggcgactcc acgcaattcg tcggcgccta ccccgacgac
     3901 acccgcaatc cggatttgct catgaaagcc gccgacaccg cgcttcgcgc gggacgcccg
     3961 gagtcggcca cggacctggc gcgccgtttg gtcaaagcct atcccaccag tcgatggacc
     4021 gcgaccgcaa accgcctcat cggtcaaggg ctgtatgaca gcggtcgttt tggagacgcc
     4081 gagcaagcgt ttcgtcgcgc cttgtcaggc gccaacgcgc aacagtccgc gaccgtggcc
     4141 gcgctcgccg cgtcctcgct gtaccaggcg gcctcgcgcg atcgaaccgc cggtcgcgac
     4201 accgaggcga tcgcgggatt cgtccgcctg gcctccgatt atccgtccac cacgttggct
     4261 cccgcggctc tgaacgaagc cgccgagctc cacgcccgat ccggcaagcc cgccgacgcc
     4321 tcggccttgt ggcagcgcct cgccgagcaa tacgccgaca gccccgaagc ccccggcgca
     4381 tttcggcgac tcgccgcttc cgcggaaacc tccggcgacc tcgccgccgc catcaagtgg
     4441 tacggccgtc tggccgagcg cagcgagccc ggcccgcgtg acgaactcac gtggaccatc
     4501 gctgcgctcg ccgaacaggc gcgcgactgg ccccgcgccg aacaaaccct gagcgcgttg
     4561 gccgcgcggt cggacttgcc gccggatcga gcgattgaag cggggtttcg cgccggtcgc
     4621 gccgcagcca accagcgccg caccacggcc acggcgctca ccgacgccgc cctcgcccgc
     4681 tatcgcgtct ggcgcggtca acccgggcct cacgagacca cgcaggccga cgtgctcgcc
     4741 gctcaaacct tggtcgccct gggcgatcaa caggccgcag cctgcgccgc ggttcgcctg
     4801 aaggaccccc tggagcaatc cctcgcccag aagcgcgccg cgctcaatgc cgccttggcg
     4861 gtctacgccg aggccgcaga aatcaaaatc gcggcaacca ccacggaggc cacgcacaag
     4921 atcggcgagg tcctgaatga gttttttcac gcgctgctcg cctccgatcg gcccggcaac
     4981 ctcaccgagg aacagctcga acagtacaat tttctgatcg aggaacaagc cgcgcctttc
     5041 gaggaacgcg cggtatccgc gtatgagacc aacgtccggc gaacgcagga attgggattg
     5101 tccgatgcct ggatcaccga gagttatgaa cgcctggccg acctgcggcc ggctcggtat
     5161 cgcaggtccg aacggctgga gctgctgcgc cggagcatgg aggccgcgcc atgaccgctc
     5221 ggcccgtcac ggtgttcacc atgaccgtct gcgccttcct cgccgcgtgc agcggcgccg
     5281 cggtgcggcg ggagccgccc gcgaccgtca cggccgcgcc cgacccggcc gcgctcttta
     5341 cccgagccct cacggcgcag gagtccggaa acgcggagcg tgcccaagcc ttgtggcggc
     5401 aactgatcgc ggcggcgccg actcaaagcg cggcccatac gaatctgggc atcctgcttc
     5461 gacaggccgg gaacaccgac gaggcgattc gcgagtacga aacggcgatc aggctcgatc
     5521 cgcgtgacgc gtgggcctat cacaacctgg gagtcgcgca tcgcctgcgc ggcgcctggc
     5581 aggacgccga gcgcgcctac cggcgcgcga tcgagctgcg tccggatcaa atggactcgc
     5641 attataatct cggcattttg tacgaactgt atctgaaccg tccggaggag gcgttgaccc
     5701 agtaccgcgc cgtgctgtcg cacggcggtc ccgacgccga caccgtggcg ccgtgggtcc
     5761 gcgcgctgga gcggcggctc gcgccggccc ctgatgcccc gaccccggag accagatgac
     5821 ccttcgttca ctcatcgccc tttgcaccac ggtcgccgtt gtatggtccg ccggaccggc
     5881 gttggccgcg tcgccggcgt ccggctcgcc tgcccaaagc ccagcgccgc aaccgcccac
     5941 agcccaagcc ccggcgccgg ccaaacccaa gaccaaaccg cgtcgggtca ttattttcgg
     6001 caccacgatc gtcggagacg tgctcactcc gcccatcgag aagaccgtgc cgtggcaacg
     6061 ccccgctgat tttcgcgccg acacggcgcc gttgacgcac gatttcacca ccgaactgtt
     6121 gactcctctt gaccgcaatg cgattcttcg tgaggctggc gaaaacgatc attaatcaac
     6181 tattctgagg tgattttttt atgtcgacgg agggaacctg aatggaaatc gtccagttct
     6241 tggtcgccaa cttcaagtcg ggcggcatct tcatgtacgg cattttggcg gtcatggtcg
     6301 tcgggacggc gatcgtgatt gaccgcacct tggccctcgt gattcgcggc cgcgtgaacg
     6361 ccgggctgtt ctggcaggag atcgacgcct tgctccaaca aggcaaactg gcggacgccc
     6421 tccagcgcgc cgacggatcg gccacgcccc tagggcgggt cctggcgtct gggttgaccg
     6481 ccgcccagcg cggggagggg cgcgagggca tcgaccggcg catcgaagaa gccgtccggt
     6541 cggtcctccc gcagctcgag aagcgcatcc actacttgta tacgctgtcc aacgtctcga
     6601 cattggtcgg gctgctgggc acggtcgtgg gcttgatcac ggcgttcacc gcggtgtcgc
     6661 tcgccgaccc cgcgcaaaag gccgcgctcc tggccaacgg gatttccttg gcgctcaaca
     6721 acaccgcgtt cggattgatg gtggcgatcc tgttgatgct ggcctattcc tacatgcagt
     6781 cgcgcgccgc gcgcctgggc gacgaaatcg acgactattc gctccgcctg gcgaaccggc
     6841 tcagcgaacg cgcggggcgt tgatgctccc gcgtttggcc agccgacggc ggcgacccgt
     6901 cgcggacgaa gccatccaca tcgtggcgct ctggaacctg atgatcattc tcatcccgtt
     6961 cctgctcttg tccgccgtgt tctcccaaac gtcgattctc aacctgtatc tgccgccgcc
     7021 acagggcgcg cccgcgccgg gaagcgcgca actggaccgc gctcccaagc ccgtggtgtc
     7081 gattctgcac gacggatacg tgctcagcga cgggccccaa atcatcgcgg ccattccctt
     7141 ggatgaacag ggtgcataca acacccgcaa actcaccgaa ctgttggtga gtatcaagcg
     7201 gtcgatcccc gcgcagcaag acatcgtgat tctcagcgaa gccgagacct cctatgaaac
     7261 cgtggtccac accatggacg cggcgcgcga atgggtctcc ctcgagggcg gccagccgct
     7321 ctttccgaac atttccctcg ggcgggtcgg gggcgccaca ccggccccgc tgcgcagcgt
     7381 tccgtgagac gcgaagccgg cacgcggcgg catctggagc cgcccagact caacctcacg
     7441 gccatggtgg acgtcttcac cgtgctcttg gtgttcttgc tcaagagcta ctcggctgaa
     7501 ggcacgctga ccgcggcggc gcaaaacctc gaactgccgt cctccacgtc cagcatcgct
     7561 ccgcagccca cgattacgat cacgatctcc gaggacacgt tgttcgtcga ggacgaacgg
     7621 gtcggggcgc tcgccgagtt cgccgaacaa tccgggccgg acatcccgga gc
//
LOCUS       DBNGTT010000068         5739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_142331, whole genome shotgun sequence.
ACCESSION   DBNGTT010000068 DBNGTT010000000
VERSION     DBNGTT010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_142331"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /gene="dop"
                     /locus_tag="AC16BC_01575"
     CDS             complement(<1..1342)
                     /gene="dop"
                     /locus_tag="AC16BC_01575"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HIR3240331.1"
                     /translation="MTARIVGTETEFGISIRDSAETDPVAASILLVNSVKGTPAPAAL
                     WDYENENPLVDARGFEVEGEKERPTVDYNRLLNKLLGNGGRLYVDGAHPEYSTPECAS
                     IRDVVRYEKAGERLLESCRIAADSVGGDRRFVLYKNNTDGKGNSYGYHENYLVARSIP
                     FALLVSRLVPFFVTRQIYAGAGKVGSENRGEPAEYQLSQRADFFETVVDLNTMVKRPI
                     MNTRDEPHADKARYRRLHVIVGDANMSEAATYLKVGVTSLVLAMIEHGYLSKELNLAN
                     PVKAIKAVSRDLSLQQPLELEDGRRWTAIQIQEYYLNTAQRYCAERGADAETKDILSR
                     WERVLRLLSTDPGQLVREVDWVIKRDLLRAYIDRKGCGWDDSRVRLMDLQYHDLRPEK
                     GLFAALERGRYVERVVTDEAVTAALTTPPPDTRAFFRARCLEKFPQHVYAASWAS"
     gene            complement(1339..3108)
                     /gene="arc"
                     /locus_tag="AC16BC_01580"
     CDS             complement(1339..3108)
                     /gene="arc"
                     /locus_tag="AC16BC_01580"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HIR3240332.1"
                     /translation="MTPKGGAMSESKRQSGKLNALKSTLRKLQDQLGSDEPNEPARYQ
                     RDIERLQTQVRGLEDELRKTEHSREQLDQASRQNEKLILALQDAREQIEALRQEVEKL
                     TAPPLSFAVFSDANRDGTVNIYLAGRKMKVAVHPSVNPSDLRRGQQLILNEALNVVET
                     RDFELQGEVVRLKDLLADRRAIVMLRADEERVAEIAASLDGERLSVGDHLLFDTRSGY
                     LLEKLPKSEVEELVLEEVPDVSYEQIGGLGDQLEQIRDAVELPFLHRELFAQYRLSPP
                     KGVLLYGPPGCGKTLIAKAVANSIAKKMGDATGRDVKSYFLHVKGPELLNKYVGESER
                     QIREVFKKAKEKAQEGMPVIVFFDEMDSLFRTRGTGISSDVESTIVPQFLAEIDGVER
                     LRNVIVIGASNRQDLIDPAVLRPGRLDVKIRVDRPDRRSAKDIFGKYLRADLPYHNPT
                     AEPIDALIGGLIDHVVDQMYSASEENRFLEVTYANGEKETLYFKDFVSGALIEGVVAR
                     AKKYAIKRAIQTGEGGIRTDDLVHAARDEFREHEDLPNTTNPDDWAKIAGRKSEKIMH
                     VRTMTNRSPESRKVETVTTGHYL"
     gene            complement(3161..3814)
                     /gene="thiE"
                     /locus_tag="AC16BC_01585"
     CDS             complement(3161..3814)
                     /gene="thiE"
                     /locus_tag="AC16BC_01585"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3240333.1"
                     /translation="MTPDWPLYLITDRHQVGAHGFLPTIESALQAGIRAIQLREKDLA
                     PQALLALAREVRALTARAGARLIINGRLDVMFAVDADGVHLRSESVPTHTVRRVIGPN
                     KFLGVSTHSMDEAQRAADEGADFVTFGPVYDTPSKIRYGRPVGLAALAAVCRRVRVPV
                     YALGGVSRAKIREVLGAGAYGVAMISEIMSSRDARRAAHECVEVLRAASRDGSVDHR"
     gene            complement(3896..4867)
                     /gene="thiS"
                     /locus_tag="AC16BC_01590"
     CDS             complement(3896..4867)
                     /gene="thiS"
                     /locus_tag="AC16BC_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR3240334.1"
                     /translation="MIVTLNGTRTSVGHGITVADLLAEHGIARERVAVEVNLVVIDRN
                     AFERTTLHDGDRVEVVSFVGGGMDERLRIGPVELRSRLIVGTGKFKDFAETKRVIDAS
                     GADMVTVAVRRVNILDRASENLLDYLNPKEYHILPNTAGCYTAEEAIRTARLARAAGI
                     SDLVKLEVIGDQRTLFPDNEALLDAAKVLVKEGFVVLPYTTDDPIMAKKLQDVGCAAV
                     MPLAAPIGSGLGIRNPYNLRIIRETVAVPVIVDAGVGTASDAAIAMELGCSGVLMNTG
                     IAGAKHPLLMAEAMRDAVRAGRLAYLAGRIPKKLYATASSPLDGLLE"
     gene            complement(4979..>5739)
                     /locus_tag="AC16BC_01595"
     CDS             complement(4979..>5739)
                     /locus_tag="AC16BC_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240335.1"
                     /translation="GIPVCDAAELRLKVSVPHDGAEGGTAVTRLRLGASPQASNGFDP
                     AEDLEAFSSPALSAVVRHSDYPAGQDTLWWDIRHDEFPQVWSVEIGSDQPNAVVTLSG
                     SFLSAAGQNGCLGSAWSLRDTLTGDVVDLTAGSTDYSYRNAAGIVRRFEITATQDSSS
                     PPLAPVNVWSPRQGRTSVYLAWSGATDPSLKYHVYRETDQGTIRLTTSPIIATAYLDA
                     GADRTKITTYYVTTVTKSGCESPPSEGYQLTPHR"
BASE COUNT         1051 a         1909 c         1652 g         1127 t
ORIGIN      
        1 ccgacgccca actcgccgcg tacacatgct gcgggaactt ttccaggcag cgcgcccgaa
       61 aaaacgcccg agtatccggc ggaggcgtgg tgagcgcggc ggtcacggcc tcatccgtga
      121 cgacccgctc aacgtagcgt cctcgttcga gggcggcaaa cagccctttc tcgggccgga
      181 gatcatggta ctgaagatcc atcagtctca cccgtgaatc gtcccacccg cagccctttc
      241 gatcgatgta cgcccgcagc aaatcccgct tgatcaccca gtcgacctcc cggaccagct
      301 ggcccggatc ggtcgacagc agccgcagga cccgctccca ccgcgacaga atatccttgg
      361 tttccgcgtc cgcaccgcgc tcggcgcagt agcgctgcgc tgtatttaaa taatactctt
      421 ggatctggat cgccgtccac cgccgaccgt cttccaattc aaggggctgc tgcagcgaga
      481 ggtctctcga caccgctttg atcgccttga ccgggttggc aaggttgagc tctttggaca
      541 aatagccgtg ctcgatcatc gcgagcacca acgaggtgac gcccaccttg agatacgtcg
      601 cggcctccga catattggcg tctccgacaa tgacatggag ccgccgatag cgcgccttat
      661 cggcgtgcgg ttcgtcacgc gtgttcatga tcggccgctt gaccatggta ttgagatcca
      721 cgaccgtctc gaagaaatcg gcccgctgcg agagttgata ctcggcgggc tcgccgcgat
      781 tctcggagcc gaccttcccc gcccccgcgt agatctgtcg ggtgacgaag aacggcacga
      841 gccgagagac caagagggca aacgggatcg agcgcgccac cagatagttc tcgtggtagc
      901 cgtagctgtt acctttgccg tccgtgttgt tcttatagag cacgaaccgc cggtcgcccc
      961 ccacggagtc cgccgcgatc cgacacgact ccaagaggcg ttcgcccgcc ttctcgtacc
     1021 ggacgacgtc acgaatgctg gcacactccg gcgtcgagta ctccgggtgc gcgccatcga
     1081 cgtagagacg gccgccgttc ccgagcagtt tgttcaacaa gcggttgtaa tcgacagtcg
     1141 gccgctcctt ctcgccctcg acctcgaagc cccgcgcgtc caccagggga ttttcgttct
     1201 cgtaatccca caatgcggcc ggggccgggg tgcccttcac gctgttgacg agcagaatgg
     1261 acgccgcaac cggatccgtt tccgccgaat cccgaatcga aatgccgaat tcggtttcag
     1321 tcccgacgat ccgggccgtc ataaataatg accggtcgtg acggtttcaa ccttgcggga
     1381 ctccggcgac cgattcgtca tggtacggac atgcatgatc ttttcgctct tgcgacccgc
     1441 gatctttgcc caatcgtcgg ggttggtggt gttgggaaga tcctcgtgct cgcggaactc
     1501 atcacgcgcc gcatgaacga gatcgtccgt ccgaattccg ccttccccgg tctggatcgc
     1561 ccgcttgatc gcgtatttct tcgcccgcgc caccacccct tcgatcagcg ccccgctgac
     1621 gaaatccttg aaatagagcg tttccttctc gccgttcgca tacgtcacct ccaggaaacg
     1681 attctcctcc gaggcgctgt acatctggtc gactacgtga tcgatcaacc cgccgatcaa
     1741 tgcgtcgatc ggttcggcgg tcgggttatg gtatggcaaa tccgcacgca ggtacttgcc
     1801 gaaaatatcc ttggcggacc gacgatccgg tcggtccacc cggatcttga catcgagccg
     1861 gcccgggcgg agcaccgcgg gatcgatgag atcctgtcgg ttgctggcgc cgatcacgat
     1921 cacgttgcgc aatcgttcca ccccgtcgat ctcggccaga aactgcggca caatggtcga
     1981 ctccacgtcg gatgagatac ccgtgcctct ggtgcgaaac aacgaatcca tctcgtcgaa
     2041 aaagacgatg acgggcatgc cttcttgcgc cttctccttg gccttcttga agacctcccg
     2101 aatctgccgt tcactttcgc cgacgtattt attgaggagt tccggccctt tgacgtggag
     2161 gaaatagctt tttacgtcgc ggcccgtcgc atcgcccatc ttcttcgcga tcgagttcgc
     2221 gacggccttc gcgatcagcg tctttccgca gccgggcggt ccatataaca agacgccttt
     2281 gggtggcgac agccggtatt gggcaaacag ttctcgatga aggaacggca gctcgacggc
     2341 gtcccgaatc tgttcgagtt ggtcgccgag cccgccgatc tgctcgtagc tcacgtcggg
     2401 gacttcttcc aagacgagct cctcgacctc ggacttcgga agcttctcca gcaggtaccc
     2461 ggagcgtgta tcgaagagca gatgatcccc cacgctgagc cgctcgccgt ccagcgaggc
     2521 cgcaatttcg gccacccgtt cctcatccgc ccgcaacatg acgatcgcgc ggcggtcggc
     2581 gaggaggtct ttgagacgaa ccacttcacc ttggagctcg aaatcgcgcg tctccaccac
     2641 gttcagcgct tcgtttaaaa tcagttgctg cccgcggcgc agatccgacg ggttcacgct
     2701 cggatgcacc gcgaccttca ttttgcgacc ggcaagatag atgttgaccg tgccatctcg
     2761 attcgcgtcg gaaaacaccg cgaacgacag gggcggagcg gtcagctttt cgacttcctg
     2821 gcgcagggcc tcgatctgct cacgcgcgtc ctggagcgcc aggatcaatt tttcgttctg
     2881 acgcgaggcc tggtccaatt gctctcgcga gtgttcggtc ttccggagct cgtcttccag
     2941 gcctcgaacc tgggtctgaa gacgttcgat atcgcgctgg tatctggcgg gctcgttcgg
     3001 ttcgtcgctt cctaactgat cctgcaactt acggagggtg gacttcaacg cgttgagctt
     3061 gcctgattgg cgcttcgact cgctcatggc ccctcctttc ggggtcatac tctatcggta
     3121 tggtccatgt acagccaaca atttaatttt aacacccgcc ctatcggtgg tcaaccgatc
     3181 catcgcgcga cgcggcgcgc aggacctcca cgcactcgtg cgcggcccgt cgagcatccc
     3241 gggaagacat tatttccgag atcatcgcca ccccgtaggc gcccgctccc agcacctctc
     3301 ggattttcgc gcgcgaaaca cctccgagag cgtagaccgg aacgcgcact cgccgacata
     3361 ccgccgccaa tgccgccaga ccgaccggac gaccgtatcg tattttcgag ggtgtgtcgt
     3421 acaccgggcc gaaggtgacg aaatccgccc cctcgtccgc cgcgcgttgc gcctcgtcca
     3481 tcgaatgtgt cgataccccc aggaacttgt tcggcccgat cacgcgccgc acggtatgcg
     3541 tcggcacgct ctccgaccgg agatgcacgc cgtcggcgtc caccgcgaac atgacgtcga
     3601 gtcggccatt gatgatgaga cgagctccgg cacgcgcggt gagcgcgcgg acctcgcgcg
     3661 ccagggccag gagcgcctga ggggcgaggt ccttttcgcg aagctggatg gcgcgaattc
     3721 ccgcctgaag cgcggattcg atggtgggga ggaagccgtg cgcgccgacc tggtgtcgat
     3781 cggtaatcag gtagagcggc caatcagggg tcacaccgcc tcgtcgcgga acgcgggccg
     3841 gccgaatccc tcccgataag cctatccgac tggcgcggct tcgtcggcgg aaggattatt
     3901 cgagcagccc gtccagcggg ctcgacgccg tcgcgtagag cttcttcggg atgcgtccgg
     3961 ccaggtaagc aagccgtccc gctcggacag catcgcgcat cgcctcggcc atgagcagcg
     4021 ggtgcttggc gccggcaatc ccggtgttca tcaggacccc cgaacaaccc agctccatgg
     4081 caatcgccgc gtccgacgcg gtgcccaccc cggcatccac gatcaccgga acggcgacgg
     4141 tctcccggat gatgcggaga ttatatggat tgcggatccc caagccggac ccgatcggcg
     4201 cggccagcgg catgaccgcc gcgcacccga cgtcctggag tttcttcgcc ataatggggt
     4261 cgtccgtggt atagggtagg accacgaacc cctccttgac gagcaccttc gccgcatcga
     4321 gcagcgcctc gttatcggga aacaacgtcc gttggtcgcc gatcacctcc aacttcacga
     4381 gatcggagat cccggccgct cgcgcgagac gggcggtccg aatggcctct tccgccgtgt
     4441 aacaccccgc agtgttcgga agaatgtggt actccttcgg gttcaagtag tccagcaagt
     4501 tctcgctggc ccgatcaaga atgttcaccc gccggaccgc gacggtgacc atgtccgcgc
     4561 ccgaggcatc gatcacccgt ttggtttcgg cgaagtcctt gaatttcccg gtgcccacga
     4621 tgagccggga tctgagttcc accggcccga ttcgcagtcg ttcatccatg ccacccccca
     4681 caaaactcac gacttcgacc cggtcgccgt cgtgaagcgt cgttcgctcg aacgcgttcc
     4741 gatcgatcac caccaagttc acttctaccg ccacccgctc ccgagcgatc ccgtgttcgg
     4801 cgagtaagtc ggccacggtg atcccgtgac cgaccgacgt ccgcgttccg ttcaacgtca
     4861 cgatcatgcg gtcggcgtcg gcggtgtggc tgtgatgtcg catcgggaag ctgtgcggta
     4921 aaccgtgcag cagaagaatt ataccgaccg ctaaaaattg aagtcaaacc agacgtgatc
     4981 agcggtgggg cgtcagttga taaccttcag acggcgggct ctcgcatcca ctcttcgtca
     5041 ccgtcgtcac gtaataggtc gtgattttcg tgcgatccgc accggcatcc agatacgcgg
     5101 tcgcgatgat cggtgacgtc gtgagtcgga tcgtcccttg gtcggtttct cgatacacgt
     5161 gatatttgag cgagggatcg gtcgcacccg accacgccag atagacgctc gtccgtccct
     5221 gacgaggact ccacacgttc accggtgcca gcggcggaga cgacgaatct tgggtcgccg
     5281 tgatctcaaa gcgcctgacg attcccgcgg cgttccgata cgagtagtcc gtcgagcccg
     5341 cggtcagatc caccacgtcg ccggtcagcg tgtcgcggag cgaccacgcg ctccccaaac
     5401 acccattctg tccggccgcc gagaggaacg atccgctcag cgtgaccacc gcgttgggtt
     5461 gatcggagcc gatctcaacg ctccatacct gcgggaattc atcgtggcgg atatcccacc
     5521 acaacgtgtc ttggccggcc ggatagtccg aatggcgaac cacagcgctc agcgccggcg
     5581 aagaaaacgc ctccaaatcc tcggccggat caaacccgtt gctcgcctga ggcgacgcgc
     5641 caagtcgcag gcgcgtgacg gccgttcccc cctctgcgcc gtcgtgaggc accgagactt
     5701 tcaaccggag ttccgccgcg tcgcacaccg ggatgcccg
//
LOCUS       DBNGTT010000069         9264 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_142516, whole genome shotgun sequence.
ACCESSION   DBNGTT010000069 DBNGTT010000000
VERSION     DBNGTT010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_142516"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="AC16BC_01600"
     CDS             <1..1104
                     /locus_tag="AC16BC_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR3240336.1"
                     /translation="REIGFCQGIENYSRHLSDRAPGEPPPTLIDYFPKDFLLIVDESH
                     VTIPQVGGMYEGDRSRKKNLVEYGFRLPSAFDNRPLKFDEFDRLLSRVLYVSATPGSY
                     ELEQAGGRVVEQLIRPTGLIDPQIEVRPATGQVDNLLGEITERVKRRERVLVTTLTKR
                     MAEDLTDYYRDVGIRVQYLHSEVDTLERMEIIRELRLGKFDVLVGINLLREGLDLPEV
                     SLVAILDADKEGFLRSRTSLVQTAGRAARNVNGRVIMYADHVTGSMLGAIDETARRRT
                     VQDAYNREHGITPASIQKEIPRTLYEVSEADYVTVPIAAEPAASYVPPDELPALLKTL
                     ERQMKDAAKRLDFETAAQLRDRIEALKEASLAT"
     gene            complement(1167..1772)
                     /locus_tag="AC16BC_01605"
     CDS             complement(1167..1772)
                     /locus_tag="AC16BC_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240337.1"
                     /translation="MRNVIRGVGLAVVVIGIASAAMGDEGHTMGKVKNGIDGMSQDAL
                     IKLSLSAAPPHIAKDAGVMIPGADGKMMEVKKGSNGFTCIPTVNGRETPDPMCMDAAV
                     GQWVDSLINKAPKPSNTVPGISYMGRGGYHWEKDGAILMEEAPGAKLVKEPPHWMLMW
                     PFDAKAAGLPTMPNRGGVYVMFDGTPYAHLMVEQDPAKIKP"
     gene            1914..7499
                     /gene="uvrA"
                     /locus_tag="AC16BC_01610"
     CDS             1914..7499
                     /gene="uvrA"
                     /locus_tag="AC16BC_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3240338.1"
                     /translation="MSDDVIDIRGARQHNLKNLDLRLPLHELIVVTGVSGSGKSSLAF
                     DTIYAEGQRRYVETFSPYARQFLDRMDKPAAERIDGIPPAIAIDQTNPVRTSRSTVGT
                     MTELNDHLKLLFARAARLYCRGCGRAVTRDTPTSIVETVLASCEGADAIIAFPVRVPD
                     NFTPAEVKDLLAGQGYTRILREDGALLEVVADRGRVAPAQRGRIVEAIEAALGHGHDR
                     VSVYPLDREGRIREPRRFSAGLHCPDCDMAYRDPGPGAFSFNSPVGACEACRGFGRII
                     GLDYGLVIPDESRSLADGAIKPWQSESYAECQDDVVRFAKRRGIPTAVPWRELSPEQR
                     AWVLEGEGSWEDGKWYGVQRFFAWLETKSYKMHIRVLLSKYRAYTLCPTCRGARLKTE
                     SLLWRLGTTEDANAVMGADQRFTPVGVKPSNARWSDLPGLTIHDVMLLPVARCRAFFE
                     RITLSTPLDEAADVLLNEIRSRLRYLVDVGLGYLTLDRQSRTLSGGEVQRINLTTALG
                     TSLVNTLFVLDEPSIGLHARDMGRVISVLRRLRDAGNSLLVVEHDPQVMLAADRVLDL
                     GPGPGERGGEVVFSGTPSALIKSSRSLTAQYLAGRKTVAALTTPPPPTTDHIDVMGAS
                     ENNLRDLDVRIPLNRLVCLTGVSGSGKSTLMQDVLYRALRKLKGRPTEPPGQHRAIRG
                     HERIADVVLVDQSAIGKTTRSNPASYVGAFAAIRDLFAAEPLAIERGYRASTFSFNAG
                     TGRCPACGGNGFEHVEMQFLSDVYLRCPDCDGRRYRAEVLDVTMWPTSGEGAPQSIAD
                     VLDMTVSEALAFFAGSAEVQRALEPLVAVGLDYLRLGQPTPTLSGGEAQRLKLAGHLA
                     EARARRTRTAPILFLFDEPTTGLHFDDIAKLLTALRRLIAEGHSVVVIEHNLEVIRAA
                     DWIIDLGPEGGEEGGRVVCEGAPIDVAACTGSHTGAALRNSGPPGIADSLREPLGRHS
                     RAGGNPEDSQVTGSPPARGRQTKTPGSFPNEIRIHNAREHNLRGVDITIPREQFTVIT
                     GVSGSGKSTVAFDILFAEGQRRYLESLNAYARQFVQPAGRPDVDAIFGIPPTVAIEQR
                     TSRGGRKSTVATLTEIYHFLRLLFVKLGTPHCPDCDLAIEPQSSEAILARIVKQHRGR
                     IIHVLAPLVTARKGLYTDLAHWAKAKGFKQLRVDGVFVPTDPWPRLDRFKEHTIELPV
                     GEVVATPKTERQVREVLERALGFGKGMVRIAPGETLFSTRRACPRCGRSFAELDPRLF
                     SFNSKHGWCTGCYGTGLELRGFDAEQTGEEVWWNAWWEGAETACRTCDGRRLNPEALA
                     VRFRGKNIADVTALPVEQTEQFIGGLKLAGREAAIARDILVELRSRLAFLKEVGLGYL
                     SLDRSAPTLSGGEAQRIRLASQLGSNLRGVCYILDEPTIGLHPRDNARLLSTLHRLQA
                     KGNTVVVVEHDEDTIRSAEHVVDLGPGAGANGGRVVAQGSVRAVMRAAESLTGRFLAD
                     PLRHPLIARRAVNATDPAITIMDAVRNNLKRLRVRVPLGRLVCVTGVSGSGKSTLVRD
                     ILHDNLRVALAQSRARRTARPAWNGCRDIRGWAAVQRVLEVDQTPIGKTPRSCPATYV
                     GFWDEIRRLFAAVPEARMRGWGPSRFSFNVTGGRCDACEGQGIKTIEMSFLPDVKVAC
                     DVCRGARFTAETLTVRYQDKTIGELLAMSVDQAAKVFAAHRRIHHALRLLQDVGLGYL
                     TLGQQSPTLSGGEAQRIKLVTELAKARDDESSRSASHPHTLYVLDEPTIGLHMADVEK
                     LIRVLHRLVSAGHTVVIIEHNLDVIAEADWIIDLGPEGGSGGGRVVAQGTPEAVARAR
                     PGSHTAQFLGRFLKERRVS"
     gene            complement(7512..8924)
                     /locus_tag="AC16BC_01615"
     CDS             complement(7512..8924)
                     /locus_tag="AC16BC_01615"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3240339.1"
                     /translation="MLTVAVEILLALAGLLSAGHAFLYKRDPKSAWGWVAVCLMFPGG
                     GAALYWLMGVNRIRTRARGWVAEGRFERPGSHSAPQRCVPPFCDESLAALVRIGDTVT
                     GLPLLGSHRITILHNGEEAYPAMLRAIDNASERVCLSTYLFRSDAVGEQFLDALTRAA
                     ARGVDVRVLIDGVGELYTYPWVTTRLRRAKVRAARFLPLVRSGFHLNLRNHRKLLVAD
                     GLLGFTGGMNIGAYQLAEDSGNPRRVVDLQVAVEGPAVGQLEETFLSDWRFTTGEPRI
                     TLPARPPSAPAGQAFCRGISAGPNEDFEDLYWLVLGALGAGRRHIRIMTPYFIPDRAL
                     LAALTTAALRGVRVDIVLPQRNNLPYVAWAMRAFLWEVLQYGARVYYQPGPFAHSKLL
                     AVDDVYTMVGSANLDPRSLRLNFEFNLEIYDRDTAAAFARHFDASRDRSREVTLADMD
                     GRPLPERLRDAAAKLFAPYL"
     gene            complement(9002..>9264)
                     /locus_tag="AC16BC_01620"
     CDS             complement(9002..>9264)
                     /locus_tag="AC16BC_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3240340.1"
                     /translation="SGLLLGLVNAVVRPVLILLTLPLTILSLGLFLLVINALMVLLVA
                     WLVSGFTVEGFWTGFFLAVFLSVFGWVTNLLLGRYTVAVKID"
BASE COUNT         1645 a         3025 c         3078 g         1516 t
ORIGIN      
        1 cgggagatcg gtttttgcca gggcatcgag aattactccc gccatttgtc ggaccgcgcg
       61 cccggcgagc ctccgcccac gttgatcgac tacttcccca aggactttct gctgatcgtg
      121 gacgagagcc acgtcacgat cccgcaggtc ggtggcatgt atgagggcga ccgctcgcgc
      181 aagaagaacc tggtcgaata cgggtttcgc ctgccctccg cgttcgacaa ccgtccgttg
      241 aaattcgacg aattcgaccg cctgctcagc cgggtgttgt acgtgtcggc cacgcctggc
      301 tcctatgagc tggaacaagc cggcggacgg gtggtggagc agctgatccg gcccaccgga
      361 ctcatcgatc cccagatcga agtccgcccg gctacgggtc aggtggacaa tctactgggc
      421 gaaatcacgg aacgggtcaa gcggcgggaa cgcgtattgg tgaccacgct gaccaaacgc
      481 atggccgaag acctgaccga ctactaccgc gacgtgggca tccgcgtgca atacctgcac
      541 tcggaagtcg acacgttgga gcgcatggaa atcatccgcg agttgcgctt gggcaaattc
      601 gacgtgctgg tggggatcaa cctgttgcgg gaggggttgg acttgcctga agtctcgctg
      661 gtcgcgatcc tggacgcgga caaagagggg ttcctgcgct cgcgcacgtc gctggtgcag
      721 accgcgggcc gcgccgcgcg caacgtgaac ggccgcgtga tcatgtacgc agaccacgtg
      781 accgggtcca tgctgggagc gatcgatgaa accgcgcggc gacgcacggt ccaggacgcg
      841 tacaaccgcg agcacggcat cacgcccgca tcgattcaga aggagatccc acgcacgctc
      901 tacgaagtga gcgaggcgga ttacgtcacc gtgccgatcg ccgcggagcc cgccgcgtcc
      961 tacgtcccgc cggacgagct gccggccttg ctcaaaactc tcgaacgtca gatgaaggac
     1021 gcggccaaac gcttggattt tgagaccgcg gcgcagttgc gcgatcgcat cgaggcgctg
     1081 aaagaagcgt cattggcgac ctgacgtagg ggcacggcat gccgtgcccc tacacggcat
     1141 accgtgcccc cagcatggtg ccagctctat ggtttgatct tggccggatc ttgctcgacc
     1201 atcaggtgcg cgtacggcgt gccgtcgaac atcacgtaca cgcccccgcg attcggcatg
     1261 gtcggcaggc ccgcggcttt ggcgtcgaac ggccacatca gcatccagtg cggcggttct
     1321 ttgaccagct tggccccagg tgcctcttcc atcaaaatcg ccccgtcttt ttcccagtgg
     1381 taacccccgc ggcccatgta ggaaattccc ggcacggtgt tgcttggttt gggcgccttg
     1441 ttgatcaagc tgtccaccca ctgccccacc gcggcgtcca tgcacatggg gtccggcgtc
     1501 tcgcgcccgt tcacggtcgg gatgcaggta aagccgttgg atcccttttt gacttccatc
     1561 atcttgccgt ccgcgccggg aatcatcacc ccggcgtctt tcgcaatgtg cggcggcgcc
     1621 gcgctgagcg acagcttgat gagcgcgtct tgcgacatcc cgtcgatgcc gtttttcacc
     1681 ttgcccatgg tgtgcccttc atcgcccatc gcggccgacg cgataccgat cacgaccacg
     1741 gcgagcccga ctcctcgaat cacgttgcgc atgatgccct cccggtgagt gtggtgccga
     1801 cgacgtcggc gctggtgaaa accggccgca acttactaca acgcgccaat cagaatcaag
     1861 cgaacgccca gttgacatcg cccccggcgc tcgcttaaga cctgtggcac gccatgagcg
     1921 acgacgtcat cgacatccgg ggcgcccggc aacacaacct caagaatctc gacctccggc
     1981 ttccgctcca cgagctgatc gtggtgaccg gcgtgagcgg ctcgggcaag tcctcgctcg
     2041 ccttcgacac gatttacgcc gagggccagc gccgatacgt ggagacgttt tctccgtacg
     2101 cgcgccagtt cctcgaccgc atggacaaac cagccgccga acggatcgac ggtattccgc
     2161 cggccatcgc catcgaccag accaatccgg tgcgaacctc ccgatccacg gtcggcacca
     2221 tgaccgaact caatgatcat ctcaaactct tgtttgcgcg cgccgcgcgc ttgtactgcc
     2281 gcgggtgcgg tcgggccgtg acgcgcgata cccccacgag catcgtcgag acggtgctgg
     2341 cgtcgtgcga aggcgccgac gcgatcatcg cgtttccggt gcgcgtgccg gacaacttca
     2401 ccccggccga ggtcaaagac ctgctggcgg gccaggggta cacacggatt cttcgcgaag
     2461 acggcgcctt gctcgaggtc gtcgcggatc ggggtcgggt ggctccggcg caacgcgggc
     2521 ggatcgtgga agcaatcgag gctgcgctgg gccacggtca cgaccgggtg agcgtctacc
     2581 cgctggatcg ggaaggtcgc attcgcgagc cgcggcggtt ttccgccggc ttacactgcc
     2641 cggattgcga catggcgtat cgggatcccg gccccggcgc gttctcgttc aattcgcccg
     2701 tgggcgcgtg cgaagcatgc cgcgggttcg gccgcatcat cggactcgac tacggcctgg
     2761 tgatcccgga tgaatccagg tccttggccg acggcgcgat caaaccctgg cagagcgaga
     2821 gttacgccga gtgccaggac gatgtcgtgc gctttgcgaa gcggcgcggg attccgaccg
     2881 ccgtgccgtg gcgcgaattg tccccggagc agcgggcctg ggtgttggaa ggcgagggtt
     2941 cctgggaaga cggcaagtgg tacggcgtgc agcgcttctt cgcgtggctg gaaacaaaga
     3001 gctacaaaat gcacatccgg gtgctgctct ccaagtaccg cgcgtacacg ttgtgcccca
     3061 cctgccgcgg agcgcggctc aaaaccgagt ccctgctctg gcgtcttggc acgaccgagg
     3121 acgcgaacgc cgtcatggga gccgaccagc ggttcacccc tgtcggcgtg aagccgtcga
     3181 atgcccggtg gagcgacctg ccgggcctga cgatccacga cgtgatgctg ctgcccgtgg
     3241 cgcgatgccg ggcgtttttc gagcggatca cactgtcaac gccgctggat gaagccgcgg
     3301 atgtcctgct caatgagatc cgctcgcgat tgcgctatct ggtggacgtg ggcctggggt
     3361 acctcacgtt ggatcgccag tcgcgcacgc tctcgggcgg tgaggtgcag cggatcaatc
     3421 tcaccaccgc gttgggcacg tcgctcgtca acacgctctt tgtgctggat gagcccagca
     3481 tcggcctgca cgcgcgcgac atggggcgcg tgatcagcgt gttgcgccgg ctgcgcgacg
     3541 cgggcaactc gctattggtg gtcgagcacg acccgcaagt gatgctggcg gcggaccgcg
     3601 tcctggatct ggggccgggg cccggcgagc ggggcggcga ggtggtgttc tccggaacac
     3661 cctcggccct cattaagtcc tcgcgatcgc tcaccgcgca atacctcgcg ggcaggaaga
     3721 ccgtggccgc gctgaccaca ccgccaccgc cgaccacgga ccatatcgac gtcatgggcg
     3781 catcggaaaa caacctccgt gacctcgatg tccgtattcc gctcaaccgc ctggtgtgcc
     3841 tcaccggcgt gagcggctcc ggcaagtcga ccttgatgca ggatgtgctc tatcgcgcgt
     3901 tgcgcaaact caagggtcgg ccgaccgagc cgcccgggca gcatcgcgcg atcaggggcc
     3961 acgaacggat cgccgacgtc gtgctggtgg accagtccgc catcggaaaa accacacgat
     4021 cgaacccggc gagctacgtg ggcgcgttcg ccgcgatccg cgacctgttc gcggccgagc
     4081 ccctggccat cgagcgcgga taccgcgcct ccacgttcag tttcaacgcg ggcacaggcc
     4141 ggtgcccggc ctgcggcggg aacgggttcg agcacgtgga gatgcagttc ctcagcgacg
     4201 tgtacctgcg ctgcccggac tgcgacggcc ggcggtaccg ggccgaggta ctggacgtga
     4261 ccatgtggcc gaccagcggt gagggtgcgc cccaatccat cgcggacgtg ctggacatga
     4321 ccgtgtccga ggcgctcgcg tttttcgcgg gatcagccga ggtgcagcgg gcgctcgaac
     4381 ccctggtggc ggtcgggctg gactacctcc ggttgggtca gccgacaccg actctgtccg
     4441 gcggcgaagc gcagcggctc aagctggccg gccatctcgc ggaagcgcgc gcgcggcgca
     4501 ctcggaccgc gcccatattg ttcctgttcg acgagcccac caccggattg cacttcgacg
     4561 acatcgcgaa actgctcacc gcgttgcgac gtctgatcgc ggagggacat tcggtggtag
     4621 tgatcgagca caacctggag gtgattcggg ccgcggattg gatcatcgac ctggggccgg
     4681 agggcggaga agagggggga cgtgtggtgt gcgaaggcgc gcccatcgac gtggccgcgt
     4741 gcaccggaag ccacaccggc gccgcgctcc gaaactcagg ccctccgggc atcgcggatt
     4801 ctctgcgaga accgctcggt cgtcattccc gcgcaggcgg gaatccagaa gactcccagg
     4861 tcactgggtc cccgcctgcg cgaggacgac agacaaagac gcctggttca ttcccaaacg
     4921 agatccgcat tcacaacgca cgcgaacaca acctgcgggg cgtggacatc acgattcccc
     4981 gtgaacaatt cacggtcatc accggggtga gcggcagcgg caagagcacg gtggcgttcg
     5041 acatcctgtt tgccgaaggt cagcgccggt atctggaatc gctcaacgcg tacgcccgac
     5101 aattcgtgca gcctgcgggc cggcccgacg tggacgcgat ctttgggatc ccgcccaccg
     5161 tggcgatcga gcagcgcacg agccgtggcg ggcgcaagag cacggtggcc acgttgaccg
     5221 agatctacca tttcctgcgg ctcttgttcg tcaagctggg aacgccccac tgtccggact
     5281 gcgacctggc catcgagccg cagtcgagcg aagccatcct cgcgcgcatc gtcaagcagc
     5341 atcgtggccg catcattcac gtgctcgcgc cgctggtgac cgcgcgcaag ggcttgtaca
     5401 cggatctggc gcactgggcc aaggccaagg ggttcaagca acttcgggtg gacggcgtat
     5461 tcgtgcccac ggatccgtgg ccgcgcctgg accggttcaa ggaacacacc atcgaactgc
     5521 cggtgggcga ggtcgtggcg acacccaaga ccgaacgtca ggtgcgcgag gtgctggagc
     5581 gcgcgctcgg gttcggcaag ggcatggtgc ggattgcgcc gggcgagacg ttgttctcga
     5641 cccgccgcgc gtgtccgcgc tgcggccgga gcttcgccga gctggatcca cggctctttt
     5701 ccttcaactc caagcacggc tggtgtacgg ggtgttacgg aaccgggttg gagttgcgcg
     5761 gcttcgacgc ggagcaaacc ggagaggagg tctggtggaa cgcgtggtgg gagggcgcgg
     5821 agaccgcgtg tcgaacgtgc gacggccggc gactgaaccc agaggcgctc gccgtgcgct
     5881 tccgcggcaa gaacatcgcg gacgtcacgg cccttccggt cgagcagacc gaacaattca
     5941 tcgggggctt gaagctcgcg ggccgcgaag ccgccatcgc gcgcgatatc ctggtcgaac
     6001 tgcgaagccg cctcgccttt ctcaaggaag tcggtctcgg gtacctgtca ttggaccgct
     6061 ccgcgcccac gctctcgggc ggggaagccc aacgcatccg cctcgcgtca caattgggct
     6121 cgaacctgcg gggcgtgtgc tacatcctgg acgagcccac gatcggtctg cacccgcggg
     6181 acaacgcgcg cctcctcagc accctgcacc ggcttcaggc caagggcaac accgtggtgg
     6241 tggtggaaca cgacgaggac acgatccgca gcgccgaaca cgtggtggat ctgggccccg
     6301 gcgcgggcgc gaacggcggt cgggtcgtgg cgcagggctc ggtgcgcgcc gtgatgcggg
     6361 cggccgagtc cttgacgggc cggttcttgg ccgatccgct gcgacacccg ctgatcgcgc
     6421 gtcgcgccgt caacgcaact gaccccgcga tcacgatcat ggacgcggtc cgaaacaacc
     6481 tcaaacgcct gcgagtgcgc gtgccgttgg gccgcctggt gtgcgtcacc ggggtgagcg
     6541 gcagcggcaa gagcacgttg gtccgcgaca ttctgcacga caacctccgc gtcgcgttgg
     6601 cccaatcccg tgctcgtcgg acggcgcggc ccgcgtggaa cgggtgccgg gacatccgcg
     6661 ggtgggcggc ggtgcagcgc gtgttggaag tggatcagac gccgatcggc aagacgccgc
     6721 gctcgtgccc cgcgacctac gtggggttct gggacgagat ccggcgtttg ttcgcggccg
     6781 tgcccgaagc gcgcatgcgc gggtgggggc cgtcgcggtt ttcgttcaac gtgacgggcg
     6841 ggcgctgcga cgcctgcgag ggccaaggca tcaagacaat cgaaatgagc ttcctgcccg
     6901 acgtgaaggt ggcgtgcgac gtatgccgcg gcgcgcgatt caccgccgag acgctcacgg
     6961 tccggtatca agacaagacc atcggcgagt tgttggcgat gagcgtggat caggccgcga
     7021 aggttttcgc cgcgcaccgc cgcatccacc acgcgttgcg cttgctccag gacgtgggcc
     7081 tcggctacct cacgctcggc caacaaagcc ccacgctctc gggcggtgag gctcagcgca
     7141 tcaagctggt gaccgaattg gcaaaagccc gtgatgacga gagcagtcgc tccgcgtcac
     7201 acccgcacac gctctatgtg ttggacgagc cgaccatcgg cctgcacatg gccgacgttg
     7261 aaaaactgat ccgggtgctc caccgcctgg tgtcggccgg ccacacggtc gtgatcatcg
     7321 aacacaatct ggacgtgatc gccgaggccg actggatcat cgacctgggg cccgaaggtg
     7381 gatcaggcgg ggggcgcgtg gtggcgcaag gcacgccgga agccgtggcc cgcgctcgac
     7441 ccggttcgca cacggcgcaa tttcttggcc ggtttttgaa ggaacgccgc gtgtcgtgag
     7501 cggggagggt gttacagata cggcgcaaag agtttggccg ctgcgtcgcg caggcgctcg
     7561 ggcaaaggcc gaccgtccat gtccgcaagg gtgacctctc gagaccgatc gcgtgatgcg
     7621 tcgaaatggc gcgcgaacgc cgcggcggtg tcccggtcgt agatctccag gttgaattcg
     7681 aagttcaggc gcagactgcg cgggtcgagg ttggccgagc ccaccatggt gtagacgtcg
     7741 tccaccgcca acaacttgct gtgcgcgaac ggccccggtt ggtagtaaac gcgcgcgccg
     7801 tattgcaaca cctcccagag aaaggcccgc atcgcccacg ccacatacgg caggttgttg
     7861 cgctggggca gcacaatgtc cacccgcacg ccccgcagcg cggccgtggt cagcgccgcc
     7921 agcagtgccc tgtccgggat gaagtacggc gtcatgatcc gaatgtgccg tcgccccgcg
     7981 ccgagcgcgc ccaacacgag ccagtacaga tcctcgaaat cctcgttggg ccccgcactg
     8041 atgccgcggc agaatgcttg gcccgctggc gcggaggggg ggcgagccgg caaggtgatc
     8101 cggggttcac ccgtggtgaa gcgccagtcg ctcagaaacg tctcctccag ttgcccgacc
     8161 gccggcccct ccacggcaac ctgcaggtcg acgacgcgac gcggattacc cgaatcctcg
     8221 gccagttggt acgccccgat gttcatgccg ccggtgaatc ccaggaggcc gtccgccacc
     8281 aggagttttc ggtgattccg aaggttgaga tgaaagcctg agcgcaccaa gggcaaaaac
     8341 cgcgccgcgc gaaccttggc gcggcgaagt cgcgtggtta cccacgggta ggtgtacagt
     8401 tccccgacgc cgtcgatcaa cacgcgcacg tcgacgccgc gtgcggccgc cctcgtcagc
     8461 gcgtcgagga attgctcgcc caccgcgtcg ctccgaaaga gataggtcga gaggcagacg
     8521 cgttcgctgg cgttgtcgat cgcgcgcaac atggcggggt aggcttcctc gccgttgtgc
     8581 aggatggtga tgcgatggct tccaagcagc ggaagtccgg tcacggtgtc gccaatacgc
     8641 acgagcgcgg cgagcgattc gtcgcagaag ggtgggacgc accgctgagg cgcggagtga
     8701 ctgccggggc gttcgaaccg gccttccgcg acccaccccc tggcgcgggt cctgatccgg
     8761 ttcaccccca tgagccagta caatgcggcc ccgcctccgg gaaacatgag gcacacggcc
     8821 acccaccccc acgcagattt tgggtcccgt ttgtacagga aggcatggcc cgcggagagc
     8881 agacccgcga gggcgaggag gatctcgacg gcgacggtca gcatctggga cgcgtcatac
     8941 acatggtcac cgccaagccg ctgggaatga gcgggaggcg gcgtgcgggt tcacccggcg
     9001 gtcagtcgat cttgacggcg acggtataac gaccgagcaa gagattggtg acccatccga
     9061 agacggagag aaacacggcc aggaagaatc cggtccagaa tccctcgacc gtgaaccccg
     9121 acaccaacca ggccaccagc aacaccatca acgcgttgat caccaacagg aacaacccca
     9181 agctgaggat ggtgaggggc agcgtgagga gaatcaggac cggccgaacc acggcattga
     9241 ccaggcccaa caacaacccc gaca
//
LOCUS       DBNGTT010000070         8071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2186600, whole genome shotgun sequence.
ACCESSION   DBNGTT010000070 DBNGTT010000000
VERSION     DBNGTT010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2186600"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..488
                     /locus_tag="AC16BC_01625"
     CDS             111..488
                     /locus_tag="AC16BC_01625"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3240341.1"
                     /translation="MSDTEQIVRPDSSHPYLPGRQPACPLLRALSGPLEGRESSPTSD
                     RYTAGRDPQSDIVIAHPEVFRRHAGIARMAREYLPTDRKSANGVYVNNLKLDRAVLHH
                     GDVLQIGSRVVRFVWNRSKASAR"
     gene            574..2115
                     /locus_tag="AC16BC_01630"
     CDS             574..2115
                     /locus_tag="AC16BC_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR3240342.1"
                     /translation="MLLEHLPGMVYRSPADQRHKIEFASEGCHDLTGYRAKDLTTVVT
                     RPELIHPDDHQAVERQLDEALRAKRAFQVVYRMRTASGDMRWVSEQGRGVYNRRGALT
                     AVEGYVTDITEQKVAEQLLAEQAIRDSLTGLYNRRFFDERIDIELARARRNRHAVAFL
                     MCDLDHFKSVNDNRGHHVGDEVLKAVANVMMAATRGSDLIVRWGGDEIVAVLSETNEA
                     GGRIVANRIRRAVRRLSAARDIRFDVSIGIAVFPTHGESVDQIVRLADRALYIAKKSG
                     DRVHVGDEHYRLDEHAVKVVFQPIVDVWSNRVLGYEALSRDPEGRLSVAELFRKYHVV
                     GQLDELKLICFKEQLRVAGEARLNRIFLNVDFHMLEMMPPPIKPDGTDVIIEISERDA
                     LHDVDRHLEIAGKWRAHGYKFAIDDFGAGFVSLPFVARLMPEFIKIDRSLVLQAVASD
                     TFKGFSKHLLNALRTYATEGIIAEGIETERELASMKAIGIFIMQGLLLGPPQALGTDQ
                     AAAAA"
     gene            complement(2133..3536)
                     /locus_tag="AC16BC_01635"
     CDS             complement(2133..3536)
                     /locus_tag="AC16BC_01635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I 51 kDa subunit family protein"
                     /protein_id="HIR3240343.1"
                     /translation="MPGYLLPEKPITSAEEYAKTGGWRGLERALSMTPEAVIDEIKKS
                     GLRGRGGAGFPTGIKWAGTRQKESPDGRKYLVCNGAEGEPGTYKDRPLIMLNPYQLIE
                     GVAIASLTIGVDKAFICLKEIFHSQIKRLGDAIAECEAKGYLGPNVLKSGRAVHAEVT
                     IGPATYLLGEETAMLETIMGNAAMPRQVKPFEWGLPFSGGGIASPVTVNNVETLSHVA
                     HIMRNGADWFKVIGPPRSPGNFIWTVSGDINKPGFYELPMGTTMTQLLEVAGGPKPGR
                     KFKAVFPGGPSAGLLTPDKFDTPLDFDSLKTVDSGLGSGCVIVYDDTACMVHVAYNFS
                     RFFANESCGQCFSCNWSTKEITRLILKMEQGTGSEADMMEAWEITERMPGKGRCFLIN
                     AESLIVRTILKHFPGEFANHAGKSCPSRRKLPIPKILDFTGKDFVYDTMDLELDWVPG
                     QQFPVLVPDDHQRTRGI"
     gene            complement(3709..4239)
                     /locus_tag="AC16BC_01640"
     CDS             complement(3709..4239)
                     /locus_tag="AC16BC_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240344.1"
                     /translation="MISLASRIMGMALGAALAIGVSGERSAAQAASIYRGFYSLQLGM
                     TVEEFAATTPATEVSRNPQNRVFAITQVPADIYGVTASFDEDQLAHIEVIYSAAYSAR
                     TSWDDFIVIATRKYGTGFHLPTPNGDVEMWDDGRTTLILERRDVTAQAHAYTLTLVDD
                     AVALKRGGRCSPRIEV"
     gene            4823..6232
                     /gene="leuC"
                     /locus_tag="AC16BC_01645"
     CDS             4823..6232
                     /gene="leuC"
                     /locus_tag="AC16BC_01645"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3240345.1"
                     /translation="MNAKTMFDKIWDAHVVHAEAGKPSLIYIDRHLIHEVTSPQAFDG
                     LRMAKRRVRRPELTFATMDHNVPTSDRSLPITDEISKVQMDTLARNCKEFGVTLFDLK
                     SPDQGIVHVIGPELGITLPGSTIVCGDSHTSTHGAFGALAFGIGTSEVEHVMATQCLL
                     QDKPKTFLIDVNGKRPYGVEAKDVILSIIGKIGTEGGTGSVIEYAGDTIRALSMEERM
                     TVCNMSIEGGARAGMIAPDEKTFAYLKGRRYAPKDFDAAVGRWKTLPSDPGATFDRTL
                     AVDAASLAPQVTWGTNPGQVAAIDGRVPNPGEMSDPNARKAAERALDYMGLRGGMPIA
                     DLAIDRVFIGSCTNSRIEDLRRVARFVDGRRVSSKVYAMVVPGSQAIKQRAEAEGLDR
                     IFKAAGFDWRESGCSMCLGMNPDTLKPGERCASTSNRNFEGRQGKGGRTHLVSPLMAA
                     AAAVEGRFVDIRQYELKEN"
     gene            6234..6827
                     /gene="leuD"
                     /locus_tag="AC16BC_01650"
     CDS             6234..6827
                     /gene="leuD"
                     /locus_tag="AC16BC_01650"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3240346.1"
                     /translation="MEPFRTLDGVVTVLDLPNVDTDQIIPKQFLKRIERTGFGQFLFY
                     DWRFIDGKQPNPEFEMNADRYKGASILVTRANFGCGSSREHAPWALLDYGFRCVIAPS
                     FADIFYNNCFKNGILPVRLSEEQVETIFQRVRATPGYRLRVDLEQQAITSNDGITFAF
                     EVEEFRRYCLLNGLDDIGLTLQHEDKITRYEQSHVMV"
     gene            6902..7123
                     /locus_tag="AC16BC_01655"
     CDS             6902..7123
                     /locus_tag="AC16BC_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240347.1"
                     /translation="MAGNAKARFEKRQKEMKRQKKHQEKAAKQLARKEEKAKGAAGLA
                     PGEDPDLAGLVPGPNQPVPEEAADLTTSS"
     gene            complement(7162..7500)
                     /locus_tag="AC16BC_01660"
     CDS             complement(7162..7500)
                     /locus_tag="AC16BC_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240348.1"
                     /translation="MTVVDLIRRFREIPPDLHHEPVLEEFAATFGELLKIANKPSACT
                     TNYDAGHLAYMKLIAPMGIYGFRLCSREDCLQQLQMLIRQYQADPAGFRAGLVSKDAV
                     ATGDRRNERS"
     gene            7648..>8071
                     /locus_tag="AC16BC_01665"
     CDS             7648..>8071
                     /locus_tag="AC16BC_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3240349.1"
                     /translation="MILAIAAALLVLVVGGERGHAASAAVELKKGVFLIAEPSLQDPN
                     FSETVILITHHSPQGSTGVVINRPTTTLLSRALPERKELAGRSDVLHAGGPVDPRVFL
                     LLVRSRTRPGHAQHVFGDVYVTQAADALLDALKSDDETT"
BASE COUNT         1511 a         2482 c         2630 g         1448 t
ORIGIN      
        1 ggggtttgcc tggtcggcaa gcccctcgtc gtttgtgccc gacgcgaggt tgacagcgtg
       61 cccgccccgt ctatagttgg ggctggagtc gctgccgcgc gcttgaactc gtgtcggata
      121 cagaacaaat cgttcgtccg gattcctccc atccctatct gccggggcgg cagccggcgt
      181 gtccgctgct gcgcgcgttg agcgggcccc tggaagggcg tgagtcttcg ccgacgtccg
      241 atcggtacac cgcgggccgc gatccgcagt cggacatcgt gatcgcccac cccgaggttt
      301 ttcgccgtca cgccgggatc gctcggatgg ccagagagta cctcccgacc gatcgtaaga
      361 gcgccaacgg cgtctacgtc aataacttga agctggaccg cgccgtgctc caccacggcg
      421 acgtgctcca aatcggatcc cgcgtggttc ggttcgtctg gaaccgaagc aaggcgtcgg
      481 cccggtaaca tgggcgcgaa cacacctcgt aagaggccgt cgcgccgcgc aacggtccga
      541 cgccgcgccg gatcgcgttc ctccaacgcg tcggtgctcc tggaacacct tcccggcatg
      601 gtgtatcggt cgccggccga tcagcgccac aagatcgagt tcgccagcga ggggtgccac
      661 gatctgacgg gatatcgggc caaggacctg acgacggtcg tgacgcgccc ggaattgatt
      721 catcccgacg atcatcaggc cgtcgagcgt cagctggacg aggcgctgcg cgccaaacgg
      781 gcgtttcagg ttgtgtaccg catgcgcacc gcgtccggcg acatgcgatg ggtctccgag
      841 caaggccgcg gggtgtacaa ccggcgcggc gcgctcaccg cggtggaagg gtacgtcacc
      901 gacatcacgg aacagaaagt cgccgagcag ttgttggccg aacaggcgat ccgggattcg
      961 ctcaccgggc tgtacaatcg ccggtttttc gacgaacgca tcgacattga attggcgcgc
     1021 gcgcgccgga atcgccacgc ggtcgcgttc ctgatgtgcg acctggatca tttcaaatcc
     1081 gtcaacgaca accggggcca ccacgtcggc gacgaggtgc tcaaggccgt ggccaacgtc
     1141 atgatggcgg caacccgggg cagcgacttg atcgtgcgct ggggcggcga cgaaatcgtg
     1201 gccgtgctgt ccgagacgaa cgaagccgga ggccgcatcg tggccaatcg catccggcgg
     1261 gccgtgcgcc ggttgagcgc ggcgcgagat atccggttcg acgtcagtat cggcatcgcg
     1321 gtcttcccga cccacggcga gtcggtcgac caaatcgtgc gcctcgccga tcgggcgttg
     1381 tacatcgcca agaaaagcgg cgatcgggtc cacgtggggg acgagcatta ccgtctggac
     1441 gaacacgccg tgaaagtggt gttccagccg atcgtggacg tctggtccaa tcgggtgctg
     1501 ggctacgagg cgctcagccg cgaccccgag ggccgattaa gcgtggccga attgttccgc
     1561 aagtatcacg tggtcggaca actcgacgag ctcaagctta tctgtttcaa ggaacagttg
     1621 cgggtcgcgg gcgaggcgcg gctcaatcgg attttcctga acgtggactt tcacatgctc
     1681 gaaatgatgc ccccaccgat caaacccgat gggacggacg tcatcatcga gatctcggag
     1741 cgcgatgcgt tgcacgacgt ggaccggcac ctcgagattg cggggaagtg gcgcgcgcac
     1801 ggctacaagt tcgccatcga cgatttcggc gcgggcttcg tatcgctccc gttcgtggcg
     1861 cggttgatgc cggagttcat caaaatcgac cggtcgctcg tgctccaggc cgtggcgtcc
     1921 gacaccttca aggggttttc caaacacctg ctgaatgcgt tgcggaccta cgccacggag
     1981 ggcatcattg cggaagggat cgaaacggaa cgcgaattgg cctcgatgaa agccatcggg
     2041 atcttcatca tgcaaggcct tctgttgggt ccgccccagg cgttggggac ggatcaggca
     2101 gcggcggccg cgtaggtcct tcggccggcg tcctagattc cccgggtccg ttggtggtcg
     2161 tccggcacca acaccggaaa ctgctggccc ggcacccaat ccagctccag atccatcgtg
     2221 tcgtaaacaa agtccttgcc ggtaaagtcc agaatcttgg gaatcggcag cttgcgacgc
     2281 gacggacacg atttgcccgc gtggttggcg aactctccgg ggaaatgttt caggatggtg
     2341 cggacgatga gggattccgc gttgatcagg aagcaccggc ccttgcccgg catgcgctcc
     2401 gtgatctccc acgcctccat catgtcggct tcggaacccg tgccctgctc catcttcagg
     2461 atcaaccggg tgatctcctt ggtgctccag ttgcaggaaa aacactggcc gcagctctcg
     2521 ttggcgaaga atcgggaaaa gttgtacgcc acgtggacca tgcaggcggt gtcgtcgtag
     2581 acgatcacgc agccggagcc gagcccggag tcgactgttt tcagcgagtc gaaatccagg
     2641 ggggtgtcga acttgtccgg ggtcaacaat ccggccgagg ggccgccggg gaacacggcc
     2701 ttgaatttcc gtccgggctt ggggccgccc gcgacttcca gcaattgcgt catggtggtg
     2761 cccatcggca attcatagaa gcccggcttg ttgatgtcgc cggagacggt ccaaatgaag
     2821 ttgcccgggc ttcgcggcgg accgatcacc ttgaaccagt ccgcgccgtt gcgcatgatg
     2881 tgcgccacgt gggacaacgt ttccacgtta ttgacggtca cgggggacgc gatgcccccg
     2941 cccgagaacg gcagacccca ttcgaacggt ttgacctgac gcggcatggc cgcgttgccc
     3001 atgatcgtct cgagcatcgc ggtttcctcg cccagcaggt acgtggcggg tccgatcgtc
     3061 acttcggcgt ggaccgctcg tccggacttg agcacgttgg ggcccaggta tcccttggct
     3121 tcgcactcgg caatggcgtc gcccagccgt ttgatctggg agtggaagat ctccttgaga
     3181 cagataaagg ccttgtccac gccgatggtc agcgaggcga tggccacgcc ttcgatcaac
     3241 tgataggggt tgagcatgat cagggggcgg tctttatacg tgcccggttc gccctcggcc
     3301 ccgttgcaga ccagatattt cctgccgtcc ggggattcct tctgccgggt gcccgcccac
     3361 ttgatgccgg tgggaaagcc cgcgccgccg cgtccgcgca agccggattt tttgatctcg
     3421 tcgattacgg cctccggcgt catggagagg gcgcgttcca agcccctcca gcctccggtt
     3481 ttggcgtact cctcggcgga cgtaatcggt ttctcaggca gcaagtatcc gggcaccggg
     3541 gcctcctctg gtcagcacgc cgatccagcg acgtcggcgt gagtcgtaaa gccggaatta
     3601 tatcgcacag ttccccttaa accgcaatct ccgccggagg cacacaccgg gcgaaggcgg
     3661 acagaatagt cgagaagtcg cgtagaagtc aatcggtcgg agcgggggtc agacctcgat
     3721 gcggggcgaa cagcggccgc ctcgtttgag cgccaccgcg tcgtcgacca gggtcagggt
     3781 ataggcgtga gcctgcgcgg tcacgtcccg tcgttccagg atcaacgtgg tgcggccgtc
     3841 gtcccacatt tcgacgtcgc cgttgggcgt gggcagatga aagccggtgc cgtatttgcg
     3901 ggtggcgatg acgatgaagt cgtcccacga ggtgcgggcc gagtacgccg cggagtaaat
     3961 gacctcgatg tgcgccaatt ggtcttcgtc gaacgacgcg gtgaccccgt agatgtcggc
     4021 cgggacctgc gtgatggcaa agacccggtt ttggggattc cggctgacct ccgtggccgg
     4081 cgtggtcgcg gcgaactcct cgaccgtcat gccgagttgg agcgagtaaa acccgcggta
     4141 gatcgacgcc gcctgcgcgg cggatcgctc ccccgagacg ccgatcgcaa gcgccgcgcc
     4201 cagcgccata cccatgatgc gtgacgccaa cgagatcata gcgacagtgt aggacacgat
     4261 ccttggggaa ggcaacagtg gtgcgccgat tggccggttc atcggtgaaa ggcgtcggaa
     4321 caaatcggga aggtatcttc aaccggtgaa ccagtacccc ggcccgccgg ctcacggtgt
     4381 tttcgggcat cgtaggcaaa agacaacccg gaagcggatc ttgcgatcct attgcggctc
     4441 cggcgtccgc gcttccgggt ctggtggcgc tgggcgttgt cccagctccg ccgaaagaag
     4501 gggcgtggct aggacgccgc cacctcactc gccgtagggt tggtactgcc cactggacca
     4561 cctcctttca tcggcgggac actgccgcgc gcggacccag cccgcgagcc ccccttcacc
     4621 ggaccgccgc tgcccggctc ctgggtcgcc tcccaccacg ggagactcgg ggaggagtga
     4681 ctcccctgtt caaaaaaaac tatatcacag tcgcgcggcc cgtgcgactg tcgccgtcgc
     4741 atcgcgctcc ggctgatttt gccttggctc ttgtgcgata atacgcccca cgcgctagcc
     4801 gaattgagga gtgaatcgcg tcatgaacgc caagaccatg ttcgacaaga tctgggacgc
     4861 ccacgtggta cacgcggagg cgggcaagcc gtccctcatt tatatcgacc gccacctgat
     4921 ccacgaggtc acctcgccgc aggcgttcga cggactgcgc atggccaaac gccgcgtacg
     4981 tcggcccgag ctgacgttcg cgaccatgga ccacaacgtg cccaccagtg accggtcgtt
     5041 gccgatcacg gacgaaattt ccaaggtgca gatggacacg ctggcgcgaa actgcaagga
     5101 attcggcgtc accttgttcg atctgaaaag cccggaccag ggcattgtgc acgtgatcgg
     5161 cccggaactg ggcattacct tgccgggctc gaccatcgtg tgcggcgaca gccacacctc
     5221 cacgcacggc gcgttcgggg ccctggcgtt cgggatcggc accagcgaag tcgaacacgt
     5281 gatggccacg cagtgtttgc tgcaggacaa acccaagacg ttcctgatcg acgtgaacgg
     5341 gaagcggccc tacggcgtgg aagccaagga cgtgatcctg tcgatcatcg gcaagatcgg
     5401 caccgaaggc gggaccggga gcgtgatcga atacgcgggc gacaccatcc gcgcgttgtc
     5461 catggaagag cgcatgaccg tgtgcaatat gtcgatcgaa ggcggggccc gagccgggat
     5521 gatcgcgccg gacgagaaga cctttgcgta cctcaagggc cggcgctacg cgcccaagga
     5581 tttcgatgcc gcggtgggtc gctggaagac cctcccgtcc gatcccggcg cgacgttcga
     5641 tcgcacactg gcggtggacg ccgcgtcgct cgcgccgcag gtcacctggg gcacgaaccc
     5701 cgggcaggtg gcggccattg acggacgcgt gcccaatccc ggcgagatgt ccgatcccaa
     5761 cgcccgcaag gccgcggagc gcgcgctgga ttacatgggg cttcgcggcg gcatgccgat
     5821 cgcggacctg gcgatcgatc gcgtgttcat cgggtcgtgc acgaactcgc ggatcgaaga
     5881 cctgcgccgg gtggcgcgct tcgtggacgg ccggcgcgtg tcgtcgaagg tctacgccat
     5941 ggtggtgccc ggttcccagg cgatcaagca gcgcgccgag gccgagggcc tggatcgcat
     6001 tttcaaagcc gcggggttcg attggcggga atccgggtgc tcgatgtgcc tgggcatgaa
     6061 ccccgacacg ctcaagcccg gcgagcgctg cgcgtccacg tcgaaccgga atttcgaagg
     6121 ccggcagggc aagggcggcc gcacgcacct ggtgagcccg ctcatggccg cggccgccgc
     6181 ggtcgagggc cggttcgtgg acatccgcca atacgaattg aaggagaact gacgtggagc
     6241 cgtttcgtac gttggacggg gtggtcaccg tgctggatct gccgaacgtg gacaccgacc
     6301 agatcatccc caaacagttt ctcaaacgga tcgagcgcac cgggttcggg cagttcctgt
     6361 tctacgattg gcggttcatc gacgggaagc aaccgaaccc cgagttcgag atgaacgcgg
     6421 atcggtacaa gggcgcgagc attctggtca cgcgcgccaa cttcggctgc ggctcctcgc
     6481 gcgagcacgc gccctgggcc ctgctcgatt acgggtttcg ctgcgtgatc gcgccgtcgt
     6541 tcgcggacat tttctacaac aactgtttca agaacggcat cctgccggtc cgtctcagcg
     6601 aggagcaggt cgaaacgatc tttcagcgcg tgcgcgccac gccggggtat cggctgcgcg
     6661 tggatctcga acaacaggcg atcacgtcaa acgatgggat cacgttcgcg ttcgaggtcg
     6721 aagaattcag gcggtattgc ctgctcaacg gcctggacga catcggcctc acgctgcagc
     6781 acgaggacaa gattacgcgt tacgaacaga gccatgttat ggtataagca atcagagggg
     6841 catgagggga cgcggttcac cggcggtcca tgtgccgaga ccttcactga ggagacgatg
     6901 aatggcgggg aatgcaaagg cgagatttga aaagcgtcag aaagaaatga agcggcagaa
     6961 aaagcaccag gaaaaagcgg ccaagcagtt ggcgcgcaaa gaggagaaag ccaaaggcgc
     7021 ggccggctta gcgccgggtg aagatcccga tctcgcgggc ctggtgcccg gcccgaatca
     7081 accggtcccc gaagaagccg cggacttgac cacgtcgtcg taagtccgga tcgttcgtcg
     7141 cctcatccgg gtcggagacg gttaggaacg ttcgttccgc cgatctccgg ttgccacggc
     7201 gtccttcgat acgagcccgg ccctgaatcc cgctgggtcc gcctgatatt gtcgaatcag
     7261 catttgtaac tgttgtaagc agtcttcccg cgaacacaac cgaaacccgt aaatccccat
     7321 cggcgcgatc aacttcatgt acgcgagatg ccccgcgtcg tagttggtcg tgcatgcgct
     7381 gggcttattc gcgatcttca gcaattcccc gaacgtggcc gcgaactctt ccagcacggg
     7441 ttcgtggtgc aggtccggcg gaatttcgcg aaaacgcctg attaggtcga caacggtcat
     7501 ggcggtggct ccgaaatgtc ctggtagcac cagattcgat ggcgtattat atgctgccga
     7561 cgagtgaggc agcaacggag catccgggaa caagaaggag tgtcaggaac tatttcgagc
     7621 cagacgatga gaggtaacgc aagaatcatg atccttgcga tcgccgccgc gttgctggtg
     7681 cttgtcgtcg gcggtgagcg cggtcatgcg gcgagcgccg ccgtcgagct gaaaaagggg
     7741 gtgttcctca tcgcggagcc gagcctgcag gatcccaatt tcagcgagac cgtgatcctc
     7801 atcacgcacc acagtccgca aggcagcacc ggggtggtga tcaaccgtcc gaccaccacg
     7861 ctgctttcgc gggcgttgcc cgagcgaaag gaacttgccg ggcggtcgga tgtgttgcac
     7921 gcgggcggac cggttgatcc ccgagtattc ctgctgttgg tgcgctcgcg gacccggccc
     7981 gggcacgcgc agcacgtgtt cggcgacgtg tatgtcacgc aagccgcgga cgcgttgctc
     8041 gacgcgctga agagcgatga cgagacgacc g
//
LOCUS       DBNGTT010000071         2916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1931048, whole genome shotgun sequence.
ACCESSION   DBNGTT010000071 DBNGTT010000000
VERSION     DBNGTT010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1931048"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1847)
                     /locus_tag="AC16BC_01670"
     CDS             complement(255..1847)
                     /locus_tag="AC16BC_01670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR3240350.1"
                     /translation="MNAADIATQQIVRVFEERILNVSRVFDIPIVSVAKEDIPAIARY
                     IHGTAGLRGTLSMQWAVDLRPMEARSQIYCLFSLVERGAWLILLTELPDGDRLHASIT
                     PHIHAAKWYEREIRDQFGLIPVGHPDLRRLVRHEHWPKGTHALRKDFAWNHVMGRQPG
                     EYRFRHIEGEGVFEVPVGPIHAGIIEPGHFRFSVAGEPVMQLELRHFWKHRGVEKLFE
                     IRTLVDGVSLAERVSGDTSFGHSLAYCETVERLMGITVPPRARYLRTLFLELERLHNH
                     IGDVGAICNDAAYALALAHSGRIKERVMQLADRLTGSRFLRGVNVVGGTAIDLSPAQL
                     DEVIREADAIHADFGELAAIVFENASLTDRLESTGVLSEQTAWDHGVMGVVGRASNVD
                     RDVRRDHPFAAYTDLRFRVPVFNYGDVRARVRIRIEEAKESISLIRQVRERLPSGEVR
                     IPPPGPPDAGQWALGAVEGWRGEILYYVMAGENGAIHRCKVRDPSFVNWPAIQFAVLG
                     NIIPDFPLINKSFNLSYAGTDL"
     gene            complement(1851..>2916)
                     /locus_tag="AC16BC_01675"
     CDS             complement(1851..>2916)
                     /locus_tag="AC16BC_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3240351.1"
                     /translation="WKYLILGSVGIALALFGTVLMYSSSVRALGDVSAALNITELLAV
                     AGRLDPYVVKLAFVFILVGYGTKIGLVPMHTWVPEAYGEAPAPVAAMLAGVLETVAFY
                     AILRSKLVVDAVVSPDYAGNLLITFGLLSVVVSALFILVQRDYKRLFAYSSIEHMGIA
                     AIGFGVGGVGTFGALLHLVNHAFSKSLAFFAAGNIHIRFGTREIDGVRGMLKAQPWTA
                     AALLIAGLALVGMPPLAMFVSEFSILAAVATHAYAADTIHFGRFLTIMVADEVRNLAL
                     VTVFVVIAVVVFGGVMYRVAGMVWGDPPDGVARGENLGLSHLSFALSIGPLVALGVTI
                     PWPLNALMGMAVAIIRQEVG"
BASE COUNT          583 a          922 c          907 g          504 t
ORIGIN      
        1 gttgcacgat cccgtgtcca cctcgcggat cgtgagcgat ttgcggaaac aggcggcctt
       61 cttcgcggtg ttgtcggaaa tcacggggag gggcgcggtc ggctcgggat gccgtcccgt
      121 gaccacgccg gtcttcaggc ttttcttgat gattcggaac atggcgtctc ctaaggtagc
      181 tcatagctca cagctcacgg caacagaaga acgacctcga cgcggttcga atttcctgtg
      241 agctgtgagc tgctctacaa atccgtcccc gcgtacgaca agttaaagct tttgttgatc
      301 aatgggaagt ccggaatgat gttgcccaag accgcgaact ggatcgccgg ccagttcacg
      361 aacgaggggt cgcggacttt gcagcggtgg atcgcgccgt tctcgccggc catcacgtag
      421 tacaggattt cgccccgcca gccctcgacc gctccgagcg cccattgtcc ggcatctggc
      481 gggcccggag gcgggatccg tacctcaccg gaaggcaggc gttcgcgcac ttgccggatc
      541 aggctgatcg actccttggc ctcttcgatg cggatgcgca cccttgcgcg gacgtctccg
      601 tagttgaaga ccggcacgcg gaacctcaga tcggtgtacg ccgcaaatgg gtgatcgcgt
      661 cggacgtcac gatcgacgtt ggacgcccgg ccgaccacac ccatgacgcc gtgatcccac
      721 gcggtctgtt cggacaacac cccggtgctc tcgagccggt cggtcagcga agcgttttcg
      781 aacacgatcg ccgcgagctc cccgaaatcc gcgtggatcg cgtccgcctc tcggatgact
      841 tcgtcaagct gcgccgggct caggtcgatc gcggtgccgc ccacgacgtt cacgccgcgc
      901 aggaagcggc ttccggtcag acggtcggcg agttgcatga cgcgttcctt tatccggcca
      961 gagtgggcca gggccaaggc gtaggccgcg tcgttgcaga tggccccgac gtcaccgata
     1021 tgattatgca ggcgctccag ctccaggaag agggtgcgca ggtagcgcgc tcgcggcgga
     1081 accgtgatgc ccatcaaccg ttcgacggtt tcgcaatacg cgaggctgtg cccgaacgag
     1141 gtgtcgcccg agaccctttc cgccagggac acgccgtcaa cgagcgtgcg gatctcgaag
     1201 agtttttcca cgccgcggtg tttccagaag tgacgcaact cgagttgcat cacgggttcg
     1261 ccggcgaccg aaaaacgaaa atggcccggt tcgatgattc ccgcgtggat gggtcccacc
     1321 gggacttcga acacgccttc gccttcaatg tggcggaagc ggtactcgcc cggctggcgc
     1381 cccatgacgt gattccacgc gaagtccttc ctcaacgcgt gggtgccctt gggccaatgc
     1441 tcgtgacgga ccaggcggcg cagatcggga tggccgacgg gaatcaggcc gaactgatcg
     1501 cggatttcgc gctcgtacca cttcgcggca tggatgtggg gcgtgatcga cgcgtggagt
     1561 cggtcgccgt cgggaagctc cgtcaacaag atcagccagg cgccgcgctc gacgagcgag
     1621 aacaggcagt agatctgcga acgggcctcc atcggtctca gatccacggc ccactgcatc
     1681 gacagggtcc cgcgaagtcc agcggtcccg tggatatagc gcgcgatcgc cggaatgtcc
     1741 tctttggcca cgctgacaat ggggatatcg aacacgcgac tcacgttcag gattcgctct
     1801 tcgaagaccc tgacgatctg ttgggttgcg atgtccgccg cattcacaag tcaccccact
     1861 tcctggcgga tgatcgcgac cgccatgccc atcaaagcgt tgagaggcca tggaatcgtc
     1921 acacccaacg ccaccagtgg cccgatgctg agagcgaacg acagatggct caagccgagg
     1981 ttttcgccgc gagccacgcc gtcaggcgga tctccccaca ccatgcccgc cacccgatac
     2041 atgacccccc cgaagaccac aaccgcaatg accacgaaga cggtaaccaa cgcgaggtta
     2101 cggacttcat cggcgaccat gatggtcaga aagcgaccga aatggatcgt gtcggcggca
     2161 tacgcgtgcg tcgcgaccgc cgcgaggatc gagaattcgc tgacgaacat tgcgagcggc
     2221 ggcatcccga ccagcgccag cccggcgatc aagagcgccg ctgcggtcca gggctgcgcc
     2281 ttcagcatcc cgcgcactcc gtcgatttcc cgcgtcccga atcgaatgtg gatattcccg
     2341 gcggcgaaga aggccaggga tttggagaag gcgtggttga cgaggtgcaa gagggctccg
     2401 aacgttccga ccccgccgac tccgaacccg atggccgcga tgcccatgtg ctcgatgctc
     2461 gaatacgcga acaaccgttt atagtcgcgc tgcaccaaga tgaagagcgc ggacaccacg
     2521 acggacagga gaccgaatgt gatgaggagg ttgccggcgt agtcgggaga gacgaccgcg
     2581 tcgaccacga gcttgctcct aaggatggcg tagaacgcga cggtttccag gacgccggcc
     2641 agcatcgcgg cgactggagc cggcgcctct ccgtaggctt cgggaaccca ggtatgcatc
     2701 ggaaccaggc cgatcttggt tccgtagccg accaggatga acacgaacgc caacttgacg
     2761 acgtaaggat cgagtcgacc ggcgacggcc agcagttcgg tgatgttgag cgccgcgctg
     2821 acgtcaccca aggcccggac cgatgatgaa tacatcaaga cggttccgaa cagggccagg
     2881 gcgattccca ccgaacccag gatcaaatac ttccat
//
LOCUS       DBNGTT010000072         2282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1931311, whole genome shotgun sequence.
ACCESSION   DBNGTT010000072 DBNGTT010000000
VERSION     DBNGTT010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1931311"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16BC_01680"
     CDS             complement(<1..240)
                     /locus_tag="AC16BC_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240352.1"
                     /translation="MASRRIGDRVHLVLRFWIPLPAVLETDPLIQDLAAQYGAAVRED
                     RSDDAARLKQAVREAIHTAVKPLDMTSVLPRAEEGV"
     gene            complement(333..1265)
                     /locus_tag="AC16BC_01685"
     CDS             complement(333..1265)
                     /locus_tag="AC16BC_01685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3240353.1"
                     /translation="MHYRTLGKTRLTVSEVGFGGWSIGGRDYGPTYDRESVAAIRRAL
                     DLGVTFFDTADMYGDGRSERLFGEALSGLAGKVVVATKGGYDVARGLVKDFSRAHLEK
                     AVAASLKRLHVDVIDLYQLHNPAVDLLKGPRLFEIMEDFQRRGLIRHYGISVSDVESA
                     RLVMARGTAATLQLVHNLLRPQILQTITTEVERAGIGIIVRTPIEYGILSGKYRVGAH
                     FHATDHRATRWTPEQFAGLLQKVESFRFLVHGEARTMADAAVRYVLTSPVASTVIPGI
                     KTPEQIEEFVRASDPPYLPEADRDRIASVQRANG"
     gene            complement(1447..2205)
                     /locus_tag="AC16BC_01690"
     CDS             complement(1447..2205)
                     /locus_tag="AC16BC_01690"
                     /inference="COORDINATES: protein motif:HMM:NF038141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor N protein"
                     /protein_id="HIR3240354.1"
                     /translation="MIIKQCFGKLVVGMMLLGAVAFGSREASAYPLLQLDIGGGVYDL
                     ETETIVGTSDPFTLYAILTPGQDATSAQISELLARTYYISAALTPKTTPPGGSLGSFT
                     FDGTTVDATSDMTYGIPPLEANVAFDANDLSKHDIYETYFSEFSFKFDPLLTAQAYNT
                     ADDPGGPSSGTGAFYIPFAVDTTGLLAGLNLHFDLYDEVVKNKSGISDTDVDHFAPFS
                     HDAECCSTSVPEPGTLLLLGSGFVGWGVWRRWTA"
BASE COUNT          477 a          734 c          657 g          414 t
ORIGIN      
        1 cacgccctct tcggcgcgag gcaggaccga cgtcatgtcc agcggtttca ccgcggtatg
       61 gatagcctcg cgaaccgctt gcttcaaccg ggccgcgtcg tcgctccggt cctcgcgcac
      121 ggccgccccg tattgcgccg ccagatcctg aatcagcggg tcggtctcca gcaccgcggg
      181 cagcggaatc caaaaccgca ggaccagatg aaccctgtcc ccgatccgcc gactggccac
      241 gtgatacccc tcaaccgaga gccgtcgccc gcactcgcac ggggcagggg acgcgttgtc
      301 atcggaggga ccgcacagcg tccgcttttg ttctatccgt tcgcgcgttg aacactggca
      361 atccggtcac gatccgcctc gggtaaatac ggcggatcac tcgcgcggac gaactcctcg
      421 atctgctccg gcgtcttgat cccgggaatc acggtggacg ccaccggcga cgttaacacg
      481 tagcgcaccg cggcgtccgc catggtcctg gcctcaccgt ggaccaaaaa ccggaacgat
      541 tcgaccttct gaagcaaccc cgcgaattgt tcgggtgtcc agcgcgtggc gcggtgatcc
      601 gtggcgtgaa aatgagcgcc cacgcggtac ttgcccgaga ggatgccgta ctcgatgggc
      661 gtgcgcacga tgatgccgat gcccgcgcgt tccacctccg tggtaatcgt ctgcaggatc
      721 tgtggcctga gcagattatg gacgagttgg agcgtggccg cggtcccgcg cgccatgacc
      781 agacgcgcgg actcgacgtc gctgaccgaa atgccatagt ggcggatcag gccgcgacgc
      841 tggaagtcct ccatgatttc gaacaagcgc ggacctttga gcagatcaac cgccgggttg
      901 tgcaactgat acaggtcgat gacgtcgacg tgaaggcgct tgaggctcgc ggctacggcc
      961 ttttccagat gagcgcgcga aaaatccttg accagaccgc gcgccacgtc gtacccgccc
     1021 ttggtggcga cgacaacctt tcccgcgaga ccggagagcg cctcaccgaa cagccgctcg
     1081 ctgcggccgt caccatacat gtccgcggtg tcgaagaacg tgacgcccaa atcgagcgcg
     1141 cgccggattg cggccacgga ctcgcgatca tacgtgggcc cgtagtcccg cccgccgatg
     1201 gaccagccgc cgaacccgac ttcagaaacc gtcagacggg tttttccaag agtccgatag
     1261 tgcatggcgg ttcagggcgc ccactgtatc atcctgcttt gtgggaccgc aatggccggg
     1321 caaagcgcca ggttcctccg tgcgacgtcg ctacgactcg ccgagaacgc gccggggaca
     1381 caaaaagact caggggaccg aagtcccctg agtctttgat tcatgaacaa cgttggagta
     1441 tcgaacttag gcggtccagc gccgccacac gccccacccc acgaatccgg aaccgagcaa
     1501 gagcaacgtc ccgggttcgg gcacgctcgt cgagcagcac tcggcatcgt gcgagaacgg
     1561 cgcgaagtga tcgacatcag tatcgctaat cccactcttg tttttcacca cctcgtcata
     1621 caagtcaaaa tggagattaa ggcccgcgag caacccagtg gtgtccacag caaaaggaat
     1681 atagaaggcc ccggttccag acgaaggtcc acccggatcg tccgccgtgt tgtaggcctg
     1741 cgccgtcagc agggggtcga atttgaagct gaattcactg aagtacgttt cgtagatatc
     1801 gtgcttcgac aaatcattcg cgtcgaacgc aacattggct tccagcggcg gaataccgta
     1861 ggtcatgtcg ctcgtggcat caaccgtggt gccgtcaaac gtaaagcttc ccaacgagcc
     1921 tccgggcggc gtggtctttg gcgtcagcgc cgcggaaatg tagtaggtgc gcgccaacaa
     1981 ctcgctgatc tgtgccgagg ttgcgtcctg gcccggggtc aggatcgcat acaaggtaaa
     2041 cgggtccgag gtcccgacaa tcgtctcggt ctcaaggtcg tacacgccac caccgatatc
     2101 caactgcaag agaggatagg ccgatgcctc tcgacttcca aaggccacag cccccaacag
     2161 catcattccc accaccagtt tcccaaaaca ttgcttgatg atcacggaac cctccttccc
     2221 acagggggaa attaactcga tgtcacacta agttatattc aactcgtgtg ccaattatca
     2281 tt
//
LOCUS       DBNGTT010000073         4549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_654219, whole genome shotgun sequence.
ACCESSION   DBNGTT010000073 DBNGTT010000000
VERSION     DBNGTT010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_654219"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="AC16BC_01695"
     CDS             complement(<1..444)
                     /locus_tag="AC16BC_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3240355.1"
                     /translation="MSVTEVGTLRTEFRVVLIKPSHYDDDGYVIRWLRSCIPSNTLAC
                     LYAITEAVATSGRLGPDVDLPIDVYDETNQRIPVKKIIRQLTAPGARGVVCLAGVQSN
                     QFPRAMDLAREFRRAGVTVLIGGFHVSGCLSMLETLPPDLTAAMDQ"
     gene            564..1109
                     /locus_tag="AC16BC_01700"
     CDS             564..1109
                     /locus_tag="AC16BC_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240356.1"
                     /translation="MASNGRTIVGGLVGGLMMEAYSLFAWVVLPYNRAFFESIPVSEG
                     LQRLFFEHAPDLHDGMWVFGKPGSTDPGGVVFLFLNGLPSPAWDLAGGAVISLLTGLA
                     VSWIYATTASIWAPAPRKGILFVMLLGAVAAIPAQFHLAITVGQPIPFSLAMAADTVI
                     EFLLLGIVLAVVHRPRATARS"
     gene            1252..2964
                     /locus_tag="AC16BC_01705"
     CDS             1252..2964
                     /locus_tag="AC16BC_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR3240357.1"
                     /translation="MFGSLTRNDLLRDIDSLVQTISGDAVSRDVRRASRDRLARLREA
                     YRERPDLFDPATIRLLKQLAEELNAQPSAIGLTPREVLRSTFGYTEFRTGQEAIIDAV
                     LQGRDCIGVMPTGAGKSLTYQIPARILGGTTLVVSPLIALMKDQVDALTELGIRATFI
                     NTSLDPEERRARVAALRAGRYELVYAAPEGIESWLADVLAGCDIRLVAVDEAHCISQW
                     GHDFRPAYRNLSGLKSRLGGVSVLALTATATTEVGDDIERQLGMTAPMRFRGSFFRPN
                     LRLFVYRKGDGRNTRADILSLVRERADRSGIVYCLSRKSVEAIAEFLREHGVRARPYH
                     AGMDPGDRSRVQDAFRRDEIDVVVATVAFGMGIDKPNIRYVIHRDMPKSLEGYYQEIG
                     RAGRDGAASDCVLFYSWAEVASYDRFMDAVEAPEVRARARDLTREMFAWADRRACRHQ
                     TLVAYLGEKLAPCRTSCDVCSGIDLLASSRAAAAKPKRRAADATEDVTVDEALLARLK
                     SLRKQLADARGIPAYLVFSDAALIQMVERRPTDETELLAISGVGPKKLAQYGSAFLAA
                     LRRD"
     gene            3229..4374
                     /locus_tag="AC16BC_01710"
     CDS             3229..4374
                     /locus_tag="AC16BC_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR3240358.1"
                     /translation="MEYSPGLAGVPAARSKVSYLDGQKGLLAYRGVPIDVLAKQSTFV
                     ETACLLVFGDLPPRPMLDRFTADLIAHRQIKFRITDLMKCLPETGHPMDALQAAVAAL
                     GMFYPAKNVRDPEVQYNSAVRLLAKLPTIVAAYARMRRGDNPIPPQDDLDHASNFLYM
                     LTGKVPDPLIARVLDVCLILHAEHTMNASTFSGLVTASTLADAYTVVSSAIGTLMGPL
                     HGGANEEVLLMLREIGKPENARSYVERQLASGGKVMGLGHREYKVKDPRAVILQSLAK
                     ELFAKFGKSPLYEVAEQVERAALDLLAKKGVYPNVDFYSGIVYQKMGLEPDFFTPVFA
                     MARVAGWLAHWVEQLHDNHIFRPTEIYEGVLNRPYTPIEQRAAQKAV"
BASE COUNT          777 a         1510 c         1489 g          773 t
ORIGIN      
        1 ctgatccatg gccgcggtca gatctggagg gagcgtctcc agcatcgaca gacacccgct
       61 cacgtgaaac ccgccgatca acaccgtgac gcccgcacgc cgaaactcgc gcgccagatc
      121 catggcgcgc ggaaactggt tcgactgcac gcccgccagg cacaccacgc cccgcgcgcc
      181 gggcgcggtc aattggcgga tgatcttctt gaccggaatg cgttggttgg tttcgtcgta
      241 cacgtcgatc gggagatcga cgtccggtcc cagacggccc gacgtcgcca ccgcctcggt
      301 aatggcgtac aaacacgcca gcgtgttgct ggggatgcag gagcgcagcc agcggatcac
      361 gtagccgtcg tcgtcgtaat gcgacggttt gatcaggacc acgcgaaact cggtcctgag
      421 ggtacccacc tcggtcacac tcattcgggt tctccggatg tcgatggatc atacccgatt
      481 ctgggtgaaa atgggggccg ttcgtccaca ttgcgatcac caccccgctt cgctatactg
      541 ccgcgtcaac ttggagggct accgtggcgt cgaacggccg gacaatcgtc ggtggactgg
      601 tgggcggcct gatgatggag gcgtattcgc tcttcgcctg ggtcgtgttg ccgtacaatc
      661 gcgcgttctt cgagtcgatc ccggtttccg agggcctcca gcgcctgttc ttcgaacacg
      721 caccggatct gcacgacggc atgtgggtgt tcggcaaacc cggatcgact gatccgggcg
      781 gcgtggtgtt cctgttcctg aacgggctcc cgtccccggc ctgggacctg gccggcggcg
      841 cggtgatctc actgctgacc gggctcgcgg tgagctggat ctacgcgacc accgcgtcga
      901 tctgggcgcc cgcgccgcgc aaaggcattc tgttcgtgat gctgctgggc gcggtggccg
      961 cgatccccgc ccagttccat ctggcgatta ccgtgggcca gcccattccg ttttccctgg
     1021 ccatggccgc ggacacggtg atcgaatttt tgctgctggg tatcgtgctc gccgtggtgc
     1081 accgcccccg cgccacggca cgttcatagg acgcggcaac gtgttcgagg cgtccttcga
     1141 catgctcagg acgaacgaca tgggcatcac gatgcgcgca tatcccccgt tcgtggtgag
     1201 cgcgtcgaac cacgctcact cgggttcacc cctccacgag caccggtcgc cgtgttcggc
     1261 tcgctgaccc gaaacgatct gctgcgggac atcgactcgc tggtgcagac catcagcggt
     1321 gacgcggtga gccgcgacgt caggcgggcg agccgcgacc ggctcgcccg attacgcgag
     1381 gcgtatcggg agcgccccga cctgttcgac cccgccacca tccggctgct caaacaactc
     1441 gcggaagaat tgaacgccca gccctccgca atcggcctca ccccgcgcga ggtactgcgc
     1501 tccacgttcg ggtacaccga gtttcggacc gggcaggaag caatcatcga cgcggtgctg
     1561 caaggccgcg actgcatcgg ggtgatgccc accggcgcgg gcaagtcgct gacgtaccag
     1621 atcccggccc gcatcctcgg cgggaccacc ctggtcgtgt cgccgctgat cgcgttgatg
     1681 aaagaccagg tggacgcgct caccgaactg ggcatccgcg ccacgttcat caacacgagc
     1741 ctggaccccg aggagcggcg cgcgcgcgtg gccgcgctgc gcgcggggcg gtacgagctg
     1801 gtgtacgcgg cgcccgaagg catcgagtcg tggctggccg acgtgctcgc gggttgcgac
     1861 atccggctgg tcgcggtgga cgaggcgcac tgcatcagcc agtggggcca tgatttccgc
     1921 cccgcctacc gcaacctgtc gggcctcaag tcgcggctgg gcggggtgtc cgtgctcgcg
     1981 ctgaccgcca ccgccaccac cgaggtgggc gacgacatcg aacgccagct cggcatgacc
     2041 gcccccatgc gttttcgcgg gtcgttcttc cggccgaacc tccggttgtt cgtgtaccgc
     2101 aagggcgacg gccgcaacac ccgcgccgat attctctctc tggtgcgtga gcgcgccgac
     2161 cgcagcggga tcgtgtattg cctcagccgc aagtccgtgg aagccatcgc ggagtttctg
     2221 cgcgaacacg gcgtccgcgc caggccctat cacgcgggca tggatcccgg cgaccgatcg
     2281 cgggtgcagg acgcgttccg gcgcgacgag atcgacgtgg tagtggcgac cgtggcgttc
     2341 ggcatgggca tcgacaaacc caacatccgc tacgtgattc accgcgacat gcccaagagc
     2401 cttgagggct actaccagga aatcgggcgc gcgggacgcg acggcgcggc cagcgactgc
     2461 gtgctgtttt attcgtgggc cgaagtggcg agttacgacc ggttcatgga cgcagtcgag
     2521 gcgcccgaag tccgcgcgcg ggcgcgcgat ctcacccgcg agatgttcgc gtgggcggac
     2581 cggcgcgcgt gccggcacca gacgttggtc gcgtacttgg gggagaaact cgcgccgtgc
     2641 cggacctcct gcgacgtgtg cagcgggatc gacctgctcg cgtcctcccg cgccgcggcg
     2701 gccaaaccga aacggcgcgc ggccgacgcg acggaggacg tgaccgtgga cgaggcgctg
     2761 ctcgcgcggc tcaagtcgct gcgcaaacag ctggccgacg cgcgcggcat tcccgcgtac
     2821 ctagtgttct ccgacgcggc cttgatccag atggtcgagc gccgaccgac cgacgagacc
     2881 gagctgctgg ccatcagcgg ggtgggcccc aagaaactgg cgcagtacgg ctcggccttc
     2941 ctggcggcgc tgcgccgcga ctgacgcact tctcctgatt ctcggcacct gccctggcaa
     3001 ccccgttgga ggctgttcag ggaaggccag atgcaaggcg gaggaaaagc ctcgcgcgcg
     3061 gagcgtactg ggtcgtacgt gagcacgcga ggccgaccga caacgccgca gatggccttt
     3121 catggacagc ctcccgcctg gtttgacatc gcccggaggc tctcctaata ttcagcgtat
     3181 tcccccgtca cgccgacgtg gcgggtccgc ggcgaaagga gtcgtgacgt ggaatactct
     3241 cccggtctgg caggcgttcc cgcagcgcgc tcgaaagtca gctacctcga cgggcagaaa
     3301 gggctcctcg cgtatcgcgg ggtcccgatc gacgtcctgg ccaagcagag cacgttcgtc
     3361 gaaaccgcgt gcctgctcgt gttcggcgat ctccccccgc ggcccatgct cgatcggttc
     3421 accgcggacc tgatcgcgca ccgccagatc aagttccgca tcacggattt gatgaagtgc
     3481 ctgccggaga cggggcaccc gatggacgcg ctccaggcgg ccgtggccgc gctgggcatg
     3541 ttctacccgg ccaagaacgt gcgcgacccg gaggtccaat acaactccgc ggtacgccta
     3601 ctggccaaac tgccgacgat cgtggcggcg tacgcgcgga tgcggcgggg cgacaatccg
     3661 attcctccgc aggacgatct ggatcacgcc tccaatttcc tatacatgct caccggcaag
     3721 gtgccggacc cgttgatcgc gcgcgtgctg gacgtgtgtt tgatcctaca cgccgagcac
     3781 accatgaacg cctccacgtt ttcgggcctc gtgaccgcct ccacgctcgc ggacgcctac
     3841 accgtggtct catccgcgat cggcaccctg atggggccgt tgcacggggg cgcgaacgag
     3901 gaagtcctgc tcatgttgcg ggagatcggc aagcccgaaa acgcccgctc gtacgtggaa
     3961 cggcaactcg cctcgggcgg caaggtgatg ggcctcggac accgggaata caaggtcaag
     4021 gacccgcgcg ccgtgatcct gcagtcgttg gccaaggaac tcttcgccaa attcggcaag
     4081 tcgccgctgt atgaagtggc ggagcaggtg gagcgcgcgg cgctggacct gctggccaag
     4141 aagggcgtct accccaacgt ggacttttac tccggaatcg tgtaccagaa gatggggctc
     4201 gagccggact ttttcactcc ggtgttcgcc atggcgcggg tggccgggtg gctcgcccac
     4261 tgggtcgagc agcttcatga caatcatatt ttccgcccga ccgagatcta cgaaggcgtg
     4321 ctgaatcggc cgtacacccc gatcgagcag cgagcggcgc aaaaggcggt ctgacgcatt
     4381 gtcgcaccgc ccgcgattcc tgaacgagca ggatactaaa gaacttgcag gttgctcaaa
     4441 aagctcaagc cgcaaggccg cagcgaggag gaaaccggag cgtactgggt cgtacgtgag
     4501 gatttccgac aagcgagaac gcagcggatg gactttttca gcaacctgc
//
LOCUS       DBNGTT010000074         5487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_526506, whole genome shotgun sequence.
ACCESSION   DBNGTT010000074 DBNGTT010000000
VERSION     DBNGTT010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_526506"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="AC16BC_01715"
     CDS             complement(<1..723)
                     /locus_tag="AC16BC_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIR3240359.1"
                     /translation="MKSLKGIVLLVIANALIFITLSITFTVLVRFVLPAFGINLQGSV
                     NQSLLLWSFVIGFGGAFISLLMSKHLARAGLRAVRITQPRTAEEQLVYRTVEDLSRRL
                     NITMPEVWIYDAPDPNAFATGPSKNNAMVAVSTGLLRQLDQRGVKAVLGHEMGHVFNG
                     DMFATTVLMGLMNTFVYFISNIITRHVAERNQLLAFVTYFFIQTVLSFLAMIPICYFS
                     RRREFRADRYAAQVFGADAMISA"
     gene            868..1227
                     /locus_tag="AC16BC_01720"
     CDS             868..1227
                     /locus_tag="AC16BC_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR3240360.1"
                     /translation="MSRLSAWGIALVLVGAFMPVGSVRAADSAVHQVTIERDSFAFAP
                     QALAIHQGDRVRWTNNDGQMHMVVTSKPDSNGRELELYKKIDPGATLEYQFNTAAAYY
                     YYCAIHFQMWGIISVNP"
     gene            1232..3919
                     /locus_tag="AC16BC_01725"
     CDS             1232..3919
                     /locus_tag="AC16BC_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR3240361.1"
                     /translation="MGPQTLVRAILAVLMSLAAACAPRAGTVDSSAAMPEGIRVAIDQ
                     GVAAMYRRDHDEALRRFTALLDIEPSNPVIHLRLGDVYFRTARYADAVREYDAVLAAT
                     PENSEARSGWWAARLQQGGYGDEVKARVRAEVAAWLAGAPRRATRRLAAYNGYDSLHD
                     DAAARRVLLALLRDPPGGQDGEGVAAEAFEAGLGESDPEARRHLVDAVLAAYPDSVYR
                     HLLYQYHVGWLARTRPQDEFRREARRLLRRDPENRSLNFAAGYWSIELGTDLEWAVDR
                     VRRALAALDHPDVREKLPYVTMSRWRRSLDEARGRYEDALGWGLFRLGRMPEAGEAFD
                     RAERRLPRDHRLAYHRGQWLEAQGREQDALTAYRVSMETGTQVVDARVAFTRLAVKSG
                     MITEDDPAPWRGFARDAGVMTFTDITDAAGLNGATGGRVAWGDYDGDGAEDLIVDGRR
                     LFHNDGHGRFTETTQGAGLAANAGGNGAVWADVNNDGLLDLFTMGNGQPGVEGSGGRV
                     WINRGGGAFDSPDGMVPANLAHGAPTEAAAWGDADRDGFVDLYVANYEAPFDHAVSLG
                     ICAPDHLLRNRGGTRFEDVSAAAGIVSAEPMCGRGVAWGDYDNDGFPDILVTNYRLDP
                     NFLWRNRGDGTFENTARQAGVEGEEQEGAFGHSIGAEWADVNRDGRLDVFIANLAHPR
                     YIGYSDVSQWLVNAGGSPPRFEDHFDDAGLLFEETASEPAWGDYDNDGDLDLFVTAIY
                     KGRRSTLYRNDGNGRFTDVTWLAGAGVANGWGAAYADIDRDGDLDLVVGSTDGVRLLR
                     NDGNANHWLHVKPVGTVSNRSAIGARVTVTAGPLTQTAEVQGGKGTGNQHSLPVEFGL
                     GSWDQPVSVTVRFPSGRVWQGKIKPDQIVKVTEPAEASP"
     gene            3916..4302
                     /locus_tag="AC16BC_01730"
     CDS             3916..4302
                     /locus_tag="AC16BC_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3240362.1"
                     /translation="MKVRKLLHTRMRVDDIERTIAFYRDVLGLEVVERHESPRGSKLA
                     FLKAAGSEELIELCSFPASGPVEVQPDLVHLAFEVDDLDATIADLARKGVPITDGPTT
                     SSAGTRFAFIDAPERYEVELIERKRR"
     gene            complement(4316..>5487)
                     /locus_tag="AC16BC_01735"
     CDS             complement(4316..>5487)
                     /locus_tag="AC16BC_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR3240363.1"
                     /translation="GHHRPRRRLRSRLGLLLMTISLLIAVVLWLAYANGANDNFKGVA
                     TLFGSGASDYRVALRWATATTLAGSLASIVIGAELVSKFSGKGVVPDTVLADQGFICA
                     VAFAAATTVFLATRLGFPVSTTHALVGGLLGAGFAASGFGGVNVSALGTAFVAPLLIA
                     PLLAITLTTLAYPLLHRTRMALGFARETCVCVGEQVHVVAVEPRGRALAASVAATIPT
                     ITIASPQECFQRYAGRVAGVSLHSLLGRLHFLSAGAVCFARGLNDTPKVVALLIAAQG
                     LHAPFATAVVAVAMALGGWLSARRVAETMSHKITTMNHGQGLTANLATAFLMIWASRW
                     GLPVSTTHVSCGSLFGIGAVTGQALWRAIGSIALAWVTALPVAAALAAVAYLTAG"
BASE COUNT          957 a         1741 c         1946 g          843 t
ORIGIN      
        1 ggccgagatc atggcgtccg cgccgaacac ctgcgcggcg taccgatccg cgcggaactc
       61 gcgccggcgc gagaagtagc agatcgggat catcgccagg aacgacaaga cggtctggat
      121 gaagaagtag gtcacgaacg ccagcaactg attgcgttcg gcgacgtgcc gggtgatgat
      181 gttgctgatg aagtacacga acgtgttcat caaacccatc aacaccgtgg tggcgaacat
      241 gtcgccgttg aacacgtggc ccatttcgtg ccccaacacg gccttcaccc cgcgctggtc
      301 caactgccgg agcaggccgg tggacaccgc gaccatggcg ttgttcttgc tcggcccggt
      361 ggcgaaggcg ttggggtccg gcgcgtcgta gatccacacc tccggcatgg tgatgttcaa
      421 gcgccgggac aggtcttcca cggtgcgata gaccaattgt tcctcggccg tccggggctg
      481 ggtaatccgc actgcgcgca gtccggcccg ggcgagatgt ttggacatca gcaggctgat
      541 aaacgcgccg ccgaatccga tcacaaacga ccagagcaac agcgattgat tgacggagcc
      601 ctgtagattg atgccgaacg cgggcagcac gaaccgcacc agcacggtaa acgtgatgga
      661 cagggtgatg aagatcagcg cgttggcgat caccaacagc acaatgcctt taaggctttt
      721 catcggtagt cgatgtcctc ctctctttga cgacaaaccg gccgtattct aacacgaccg
      781 agtcgccttc gacaaaatcc cgcgccgcgg gttgaccggc gccgcatggc tggggtataa
      841 ggagccggtc atgtcgggag ggcgctgatg agcaggctgt ctgcgtgggg gatcgcgttg
      901 gtgctggtcg gcgcgttcat gccggtgggc tccgtccgcg ccgcggactc ggccgtccac
      961 caagtcacca tcgagcggga cagtttcgcg ttcgcgcccc aggccctcgc cattcaccaa
     1021 ggcgatcggg tccgttggac caacaacgac gggcagatgc acatggtggt gacctccaag
     1081 cccgactcca atggccgaga actcgagttg tacaagaaga tcgacccggg cgccacattg
     1141 gagtaccagt tcaacactgc ggccgcttac tactattact gcgcgattca cttccagatg
     1201 tggggcatta tttcggtcaa cccctgagcg tgtgggaccc cagacgctcg tccgcgcaat
     1261 ccttgccgtc ctgatgtcgc tcgcggccgc ctgcgccccg cgggcgggaa cggtcgattc
     1321 gtccgccgcg atgcccgagg gtatccgggt ggcgatcgac cagggcgtcg cggccatgta
     1381 tcggcgcgac cacgacgagg cgctccgtcg cttcaccgcg ttgctggaca tcgagccgtc
     1441 caatcccgtg atccacctcc ggctgggcga cgtctacttt cggacggccc gctacgccga
     1501 cgcggtgcgc gagtacgacg cggtgttggc ggcgacgccc gagaactccg aggcgcgatc
     1561 gggttggtgg gcggcgcggc tccagcaagg cgggtacggc gacgaggtga aggcccgcgt
     1621 ccgggccgag gtcgctgcgt ggctggccgg cgcgccgcgc cgggcgacca ggcgtctcgc
     1681 cgcgtacaac ggttacgaca gtctccacga cgacgccgcg gcgcggcggg tcttgttggc
     1741 gctgctgcgc gatccgccgg gagggcagga cggcgagggc gtggccgccg aggcgttcga
     1801 ggccgggctg ggcgagagcg accccgaggc ccgccggcac ctggtggacg cggtattggc
     1861 cgcgtatccg gattcggtct accggcacct cctgtaccaa taccacgtgg gctggctggc
     1921 ccgaactcgt ccgcaggacg aatttcgccg ggaggcgcgg cggctgcttc gacgcgatcc
     1981 ggagaatcgc agcctcaact tcgcggcggg atactggtcg atcgaactcg gcacggacct
     2041 ggagtgggcg gtggaccgcg tccgccgcgc gctggccgcg ctcgatcacc cggatgttcg
     2101 cgagaagttg ccgtacgtga ccatgtcccg ctggcgccgc tcgctcgacg aggcgcgcgg
     2161 gcgttatgaa gacgcgctgg gctggggatt gttccggttg ggtcggatgc cggaagccgg
     2221 cgaggcgttc gaccgcgcgg agcggcggct ccccagagat catcgtctgg cctaccaccg
     2281 cggacaatgg ctggaggccc agggccgtga gcaggacgcg ctcaccgcct accgtgtgtc
     2341 gatggagacc ggcacgcagg tggttgacgc gcgggtcgcg ttcacacggt tggccgtcaa
     2401 atcgggcatg atcacggagg acgatccggc gccgtggcgt ggcttcgcgc gcgacgccgg
     2461 ggtcatgacg ttcaccgaca tcacggacgc ggccgggttg aacggcgcga ccggcgggcg
     2521 cgtggcgtgg ggcgattacg acggggacgg cgccgaggac ttgatcgtgg acggccgacg
     2581 cctgtttcac aatgatggcc atggtcgctt caccgaaacc acccagggcg ccgggctcgc
     2641 ggcgaacgcg ggcggcaacg gcgcggtgtg ggcggacgtg aacaacgacg gacttctgga
     2701 tctcttcacc atgggcaacg gacaacccgg cgtggaggga tcgggaggcc gggtgtggat
     2761 caaccgtggg ggcggcgcgt tcgattcacc cgacgggatg gtgccggcga atctcgccca
     2821 cggcgcgccc accgaggccg cggcgtgggg agacgcggat cgcgacgggt tcgtggacct
     2881 ctacgtggcg aattacgagg cgccgttcga tcacgccgtc agcctcggga tctgcgcgcc
     2941 cgatcatctg ctccgcaaca ggggcgggac gcggttcgag gacgtgagcg cggccgcggg
     3001 catcgtgagc gcggaaccca tgtgcggccg gggcgtggcg tggggcgact acgacaacga
     3061 cgggttcccg gacatcctgg tgaccaacta ccgactcgat ccgaattttc tctggcgcaa
     3121 tcgcggggac ggcacgttcg agaatacggc gcgacaagcc ggggtggagg gcgaggaaca
     3181 ggaaggcgcg ttcggccact ccatcggcgc ggagtgggcc gacgtgaacc gcgacggccg
     3241 cctcgacgtg ttcatcgcga acctggcgca cccgcgctac atcgggtatt cggacgtcag
     3301 tcaatggctg gtgaacgccg gcggttcgcc gccgcgtttc gaggatcact tcgacgacgc
     3361 cggactgctg ttcgaggaga ccgcgagtga acccgcgtgg ggcgattacg acaacgacgg
     3421 ggatctggat ctcttcgtca ccgcgatcta caagggacgc cgcagcacgt tgtatcgcaa
     3481 cgacgggaac ggccgtttca cggacgtgac gtggctcgcg ggcgccggtg tggccaacgg
     3541 gtggggcgcg gcgtacgcgg acatcgaccg ggacggcgac ctggatctgg tcgtcggcag
     3601 caccgacggc gtgcggctcc tgcgcaacga cggcaacgcg aaccactggc tgcacgtcaa
     3661 acccgtgggc acggtgagca accggtcggc catcggcgcg cgggtgacgg tcaccgcggg
     3721 accgctcacg cagacggccg aagtgcaggg cggcaagggg accggcaacc agcactcgct
     3781 cccggtggaa ttcggcctgg ggtcttggga ccagccggtg tcggtcacgg tgcgattccc
     3841 gtccggtcgg gtctggcagg gcaagatcaa gcccgatcag atagtgaagg tcaccgaacc
     3901 agcggaggca tcaccatgaa ggtccggaag ctgttgcaca cccgcatgcg ggtcgatgac
     3961 attgagcgta ccatcgcctt ttatcgcgac gtgttggggc ttgaggtggt ggagcggcac
     4021 gagtcgccgc ggggatccaa actcgcgttt ctcaaggccg cgggcagcga agaactgatc
     4081 gagctctgtt cgtttcccgc gagtgggccc gtggaggtgc agccggatct ggtgcacttg
     4141 gcgttcgagg tggatgatct ggatgccacg atcgcggatc tcgcgcgcaa gggcgtgccg
     4201 atcacggacg ggccgaccac ctcgtcggcc gggacccggt tcgcgttcat cgacgccccg
     4261 gagcgctacg aggtcgagct gatcgaacgc aagcggcgtt gacgcgaacg accggtcacc
     4321 cggcggtgag atacgcgacg gcggccagcg cggcggcgac cggtagggcc gtgacccacg
     4381 cgagtgcgat cgacccgatg gccctccaga gggcctgccc cgtcactgcg ccgattccga
     4441 acagagatcc acacgagacg tgggtggtcg agacggggag gccccagcgc gacgcccaga
     4501 tcatcaggaa cgcggtggcg aggttcgcgg tcaatccttg gccgtgattc atggtcgtga
     4561 tcttgtggct catggtctcg gccacgcgtc gagcgctgag ccatccaccc agggccatgg
     4621 caacggccac gaccgcggtc gcgaacggcg cgtgcagccc ttgcgcggcg atcaacaacg
     4681 ccaccacttt gggcgtatcg ttgaggccgc gggcgaaaca gaccgctccc gcgctcagga
     4741 aatgcaatcg acccagcagg gagtgaaggc tcacgcccgc cacgcgaccg gcgtaccgct
     4801 gaaagcattc ttgcggcgac gcgatggtga tcgtcgggat cgtggcggcg accgacgccg
     4861 cgagcgctcg cccccgaggt tcgaccgcga ccacgtgaac ctgttctcct acacacacgc
     4921 aggtctcacg cgcgaagccc aacgccatcc gagtccggtg caggagtgga taggcgagcg
     4981 tggtcagcgt gatggccagc aggggcgcga tcaggagcgg cgcgacgaat gccgtgccca
     5041 gcgccgacac gttcacgcct ccgaagccgc tcgccgcgaa tcccgcgccc agcaatccgc
     5101 cgaccaacgc gtgggtggtg gagaccggaa atccgaggcg cgtggcgagg aacacggtgg
     5161 ttgcggcggc gaaggccacg gcgcagatga acccctggtc ggcgagcacc gtgtcgggca
     5221 ccacgccttt tcctgaaaac ttcgagacga gctccgcgcc gatgacgatc gacgcgagcg
     5281 agcccgcgag cgtcgtggcc gtggcccatc gcagcgcgac gcgatagtcg ctggcgccgg
     5341 aaccgaacaa cgtggccacg cccttgaagt tgtcgttggc gccgttggcg tacgccagcc
     5401 agagcaccac cgcgatcagc aacgagatcg tcatcaacag cagcccgagg cgggatcgca
     5461 gccgccggcg gggtcgatgg tgccctc
//
LOCUS       DBNGTT010000075         2557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_16803, whole genome shotgun sequence.
ACCESSION   DBNGTT010000075 DBNGTT010000000
VERSION     DBNGTT010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_16803"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1462)
                     /gene="alaC"
                     /locus_tag="AC16BC_01740"
     CDS             complement(284..1462)
                     /gene="alaC"
                     /locus_tag="AC16BC_01740"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HIR3240364.1"
                     /translation="MDAFSRITRLPPYVFSIVTAMKTEARRRGEDILDFGMGNPDMPT
                     PKHIQDKLIEAVRNPKNHRYSLSKGIPKLREAMASWYQRNYGVSVDPETEVVVTIGSK
                     EGLAHLALAMVGPGDVVLTPSPTYPIHFYSVIIAGGQVKSIPLRADSDFFEDMTAAYK
                     QTWPRPKMLILNFPHNPTTRVVDLGFFKQVVDFAGENQLLVVHDLAYADLVFDGYHAP
                     SFLQVPGAKNLGVEFFTLSKSYNMPGWRVGFCVGNREMVGALAKIKSYLDYGVFQPIQ
                     IASTVALNGPQECVGEITEVYRKRRDVLVNGLNRIGWAVEKPRATMFVWAPIPEPFRA
                     MGSLEFSKLLLTEAKVAVSPGIGFGEYGDDGVRFALVENEHRTRQAIQGLKKVLRRHG
                     "
     gene            complement(1733..2215)
                     /locus_tag="AC16BC_01745"
     CDS             complement(1733..2215)
                     /locus_tag="AC16BC_01745"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O-ribose)-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR3240365.1"
                     /translation="MFRVVLHHPEIPANSGNIIRLCANTGAELHLIEPLGFQLDDARL
                     RRAGLDYHDRAALRIHASLDEFLSAVKPPRVFACSTRGRRPYADAAFQPGDALLFGSE
                     THGLPAAIRQATAPECLLRIPMIPNNRSLNLSNAVALVLYEAWRQHGFAGSTDAQFHT
                     "
     gene            2410..>2557
                     /locus_tag="AC16BC_01750"
     CDS             2410..>2557
                     /locus_tag="AC16BC_01750"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR3240366.1"
                     /translation="MAQLGFKLDHVATLRQVRGGAEPNVAAAALLAEMGGADWLIVHL
                     REDRR"
BASE COUNT          481 a          806 c          804 g          466 t
ORIGIN      
        1 ccacgcggcg cgtcaacacc tcgcggttgc gctgcaggag cttgaccacg ccggagccga
       61 cggtgccgaa gccgatgaga ccgatgccga tgcgagactt catgttagag ggctgctgaa
      121 aaagtccatc tgctgcgttc tcggtcgtcg gaactcctca cgtacgacac agtacgctcc
      181 gggttcctcc tccctgcggc cttgcatctg gagctttttg agcagcccgc aggagcctgt
      241 tgcttcacgc cggtttggtc catggttcat gacacgcaag gactcagccg tggcgacgga
      301 ggactttttt taatccttga atcgcctgcc gcgtgcggtg ctcgttttcc accagcgcaa
      361 agcgaacgcc gtcgtcgccg tattcgccga acccgatgcc gggcgagacc gcgactttgg
      421 cttcggtcag cagaagcttg gagaactcca acgaccccat cgcgcggaag ggctcgggga
      481 tcggggccca cacgaacatg gtggcgcgcg gcttctcgac cgcccagccg atccggttca
      541 gcccgttgac cagcacgtcg cgccgcttgc ggtagacctc ggtgatctcg cccacgcatt
      601 cctgcggtcc gttgagcgcc acggtggagg cgatctgaat cggctgaaac acgccgtaat
      661 ccaggtagct cttgatcttg gcgagcgcgc cgaccatctc gcggttgccc acgcaaaaac
      721 ccacgcgcca ccccggcatg ttgtagctct tggacagggt aaagaactcc acgcccaggt
      781 tcttggcccc cgggacctgg aggaaactcg gggcatggta cccgtcaaac acgagatcgg
      841 catacgccag gtcgtgcacc accagcagtt ggttctcgcc cgcgaagtcc accacttgct
      901 tgaagaaccc gaggtccacg acccgggtgg tggggttgtg cggaaaattg aggatcaaca
      961 tcttgggccg cggccatgtc tgcttgtacg ccgcggtcat gtcctcgaaa aaatccgagt
     1021 cggcgcgcag cggaatgctc ttgacctggc cgcccgcgat gatcacgctg taaaagtgga
     1081 tcggatacgt cgggctgggg gtgagcacca cgtcgccggg tccgaccatg gccagcgcga
     1141 gatgcgcgag cccttccttg gacccgatcg tgaccaccac ctcggtctcg gggtccacgc
     1201 tcacgccgta attgcgctga taccacgagg ccatggcctc gcgcagcttg ggaatgccct
     1261 tggacaacga atagcggtga ttcttggggt tgcgcaccgc ttcgatgagc ttgtcttgga
     1321 tgtgcttggg ggtgggcatg tccgggttgc ccatcccgaa gtcgagaatg tcctccccgc
     1381 gccgtctcgc ctcggtcttc atcgcggtca cgatgctgaa gacgtatggc gggagccgcg
     1441 tgattcgtga aaacgcgtcc actggtcccc taaacgaaag gcgagcgccg caaccacgtc
     1501 gcaggctcgg ccaccactca aaatagtggc ctcactatac ggaaaggccc tggtattgtc
     1561 aaacgaactc agcctcaaat catggagaaa gccctcattt ttcggggggt tctggaaacg
     1621 acgaagacat accgagagaa aatgtcgatc cgtccggcgt gcggcgcgaa aggccgatcc
     1681 ggccttgttg gatgctcgcg gccgaccaaa tggagatcac cgtcagtcgt cactaggtat
     1741 ggaactgcgc gtcggtggac cccgcaaacc cgtgttgccg ccacgcctcg tacaacacca
     1801 acgccaccgc attcgacagg ttcaagctgc ggttattggg gatcatcgga atccgcagca
     1861 gacactcggg ggccgtggcc tgccggattg cggcgggcaa cccgtgggtt tccgatccga
     1921 ataacaaggc gtcgccgggt tgaaacgcgg cgtcggcgta ggggcgccgg ccgcgcgtgg
     1981 agcaggcgaa cacgcgcggc ggtttgaccg ctgacaggaa ctcgtccaac gaggcgtgga
     2041 tccgcagcgc ggcgcggtcg tggtagtcca ggcccgcccg ccgcaaccgc gcgtcatcca
     2101 actgaaaacc gagcggttcg atcaggtgca gctccgctcc ggtgttggcg cacaatcgga
     2161 tgatattgcc cgagttcgcc gggatctcgg ggtggtgcaa gacgacgcga aacatgccaa
     2221 gaaatctctt cgacccctcg accacagctt aacacagtac ggtgaccggt ctgtcctccg
     2281 gggtcaccgc gcggaatcac tcgaggcgga cgagcgcggg cggtcgccgc gatgttccga
     2341 gttttcttga ttttttcccc gcgccgatgc cacaatgccg cgcacacgcg ggggccgtac
     2401 cggcacggaa tggcgcaact cggtttcaaa ctcgatcacg tcgcaacgct caggcaggtg
     2461 cgcggaggcg ccgagcccaa tgtcgcggcc gcggcattgt tggcggaaat gggcggcgct
     2521 gactggctga tcgtgcacct gcgcgaggac cggcgcc
//
LOCUS       DBNGTT010000076         6913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2955197, whole genome shotgun sequence.
ACCESSION   DBNGTT010000076 DBNGTT010000000
VERSION     DBNGTT010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2955197"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC16BC_01755"
     CDS             complement(<1..189)
                     /locus_tag="AC16BC_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HIR3240367.1"
                     /translation="MKTVPVFVLLLALVWFAHPVARWFAAGLVLVVLGQGIRVWAAGH
                     LYKNERVVTSGPYAYVKNP"
     gene            589..1245
                     /locus_tag="AC16BC_01760"
     CDS             589..1245
                     /locus_tag="AC16BC_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3240368.1"
                     /translation="MVLGLPKVIPLFPLPDLVFFPHVALPLHIFEPRYRAMVRDALDG
                     DRIIGMTLLQDGWEESYYENTAPIHSVGCAGRITAVQHMEGGRFNITLFGLTRFTMRD
                     QFCDAPYRQAWVEPFHESDAPVPLPSDLKCSLHRLVREFGRLTGRAAEFEVFLTRMVH
                     EPAFLNICCSELRFTAVEKQLLLEATCPLQQAKRLVEILQFNIDALVRNSAPTKNVGS
                     "
     gene            1285..1707
                     /locus_tag="AC16BC_01765"
     CDS             1285..1707
                     /locus_tag="AC16BC_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HIR3240369.1"
                     /translation="MTRWFAGFVVASFVSVSAWVAVPASADGPFSAINRAPNDAHTPV
                     IDAPDAVKAGEPFTVTITVGKTPHPSQPDHAVQWIALYADEVELARATLTPTLTRPKV
                     TFTVVLDQSATLRALEAPNHSAAWEAVKKITVSKDEKK"
     gene            1710..2198
                     /gene="bcp"
                     /locus_tag="AC16BC_01770"
     CDS             1710..2198
                     /gene="bcp"
                     /locus_tag="AC16BC_01770"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR3240370.1"
                     /translation="MANQPTDGPSEGDLAPDFALAASTGRTIALHEFRGKQAVVLYFY
                     PKDDTPGCTIEACGFRDTFAAYGKAGAVILGVSLDNLNSHKKFTDKFSLPFPLLSDET
                     QSVSKAYGVYKLKKMYGKEYLGIERTTFLIDKQGRIAKIYPKVKVDEHHHEVLKDVKA
                     LK"
     gene            complement(2310..3455)
                     /locus_tag="AC16BC_01775"
     CDS             complement(2310..3455)
                     /locus_tag="AC16BC_01775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3240371.1"
                     /translation="MCYFGTYSAGAGYPVNRVLIEGLRRAGATVWECRAGAADDGEER
                     WKSAQGPLAWLRAAGRMAVAWIRLVARWISGPPTDVVIVGYLGHLDVFLARLLTRRPV
                     ALNALLSLHDTVVRDRGLASERSLAARLLRGIDRSACRAADLVLLDTNAHIAYFREQF
                     GLDHARFLRVPVGSDRSGRPAAIGPSGASVRVLFVGTFIPLHGVRTIVAAAQRVEQRG
                     LDIRFRLIGRGQEFAGAAELVGREGLTRVELVDRWINGDAYERELAAADICLGIFGAT
                     DKARRVIPSKVYDALAAGRPLITADTPAARELLTHGVDAWLCPPDDPAALADALATLA
                     GDRALRARLAEGARRTYEARCSPDAIGRDVLVALERLVRSSSPAAGC"
     gene            complement(3612..4514)
                     /locus_tag="AC16BC_01780"
     CDS             complement(3612..4514)
                     /locus_tag="AC16BC_01780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3240372.1"
                     /translation="MTEPVRRARVSLVIPVHNEAMNITDLVKRTRDVLGPEDEVLVVD
                     DGSTDGSGQLARDAGARVVSHPYRMGNGAAIKSGIRSARGSVVVMMDGDGQHNPADVP
                     RLVAQIGPYDMAVGARAAGSQSDMHRWLGNRFYNVFASYLTNRPILDTTSGFRAIKAS
                     LARRFVYLLPNAFSYPTTLTMATIRAGHSVTFVPIVTARRKGESKLQPLRDGARFLVI
                     MFKIATLYSPIKVFLPVSAAFFATGLGYYLYTFIESRRFTNMSALLFINAVIIFLMGL
                     VSEQIAQMRFEHSERLTDHDHSDS"
     gene            complement(4563..5798)
                     /locus_tag="AC16BC_01785"
     CDS             complement(4563..5798)
                     /locus_tag="AC16BC_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3240373.1"
                     /translation="MRILLVSMSCPLPLKAGGRIRVFNLLKRLATRHRITLLCLVDSD
                     EEFARHRAELDALCERVIGVRWRPGVLGLLGRAFAAGARVAQLPVVVLNKRSAELASV
                     LRGLLQRESFDVVQVEWVHMAQHVAAADRAILARRGVLVEHDVSWLPLERRAAVASGP
                     ARWFWRREARLMRAYEREQASAFRAVAAVSPDDAKRLAADGVRNVTVVPNGVDVAFYT
                     ESPAPVKDPRTLIFIGWMRHDPNVDGLLFFLNEVWPLIVQDLSDARLVVIGAPVPASV
                     RRAAARVPGVELAGYVPDVRSPLRAATVSVVPLRVGGGTRLKILESMAAGTAVVSTSV
                     GCEGLGLEPNRHLVVADDPAAFASGVVRLLRDPALRQRLERDALDAVRARYDWSRMAD
                     QMDELYQRVAADEARVSES"
     gene            complement(5804..6787)
                     /locus_tag="AC16BC_01790"
     CDS             complement(5804..6787)
                     /locus_tag="AC16BC_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240374.1"
                     /translation="MVAAVAVTIGFALPAVPTVSADHGSAAPSDGIDAHAAEASEGME
                     PSCHGTSHDVGAAIAERAATLTEAMVACSDRCTFGCVHGAFRAYFSKRLGRAGATAVA
                     PDVAQEISDLCREDSTVLPGFYRGNCAHSVGHAFAAVASDAPGAAAWCRVFEPGEMRF
                     YCETGVFMEARRDIRAGIIRERMSFAARAKAGVDYCSVHASAPGACLRFVWPWAEDAD
                     DARVLVRACGRMDGQARRGCVYAAGYVSRQYLTRHPADAGRVCGAAEPADRVYCAAGL
                     ALMKKDHHARAALPALCRGFDDRDLRAACDDQASRFYYQLDNPLIAATLPE"
BASE COUNT         1250 a         2502 c         2186 g          975 t
ORIGIN      
        1 ggggttcttg acgtacgcgt acgggccgct cgtgaccacc cgctcgttct tatagaggtg
       61 gcccgccgcc cacacgcgga ttccctgtcc gagcaccacc aggacgaggc ccgcggcgaa
      121 ccagcgcgcc accgggtgcg cgaaccacac gagcgcgagc aacaagacga acaccggcac
      181 cgttttcacg gtgatcttcc gcctcattgc ggaccaggaa agggtcgagt ccatgggatc
      241 gtcgagagtg aaaccgaggg tggagcgagt ataggcaaac acctccgaaa attcaaccta
      301 cgccgcgtca cgcagtgacg cggaatccga tgggcgaagg ccccgactcg agtggcggag
      361 caacctgagc cgaccgcctc cgcgcgcgtg ccggagcgac ttccaccggg tcacgcggtg
      421 acgcggaatc cggcgggcga aggccgcgtg atcggcggcg aggcgacccg cggcgcgcca
      481 cgcaggaaac ggagacgacg aacggacgcg cctcagccgc cgcgttgcct tgacattttc
      541 tcgacgcgat ttgtagagta ccgttgcttc gatcgcttgg aagacgcgat ggtgctcggc
      601 ctcccgaaag tcatcccgct gttcccgctc cccgacctcg tcttctttcc ccacgtcgcg
      661 ctgccgctgc atatcttcga accccgctac cgggccatgg tgcgcgacgc gctcgacggg
      721 gatcgcatca tcggcatgac cctgctgcag gacggctggg aggagtccta ttacgagaac
      781 accgcgccca ttcattcggt cgggtgcgcg gggcgcatca ccgcggtgca gcacatggag
      841 ggcggacgct tcaacatcac gctcttcggg ctcacgcgat ttacgatgcg cgatcaattc
      901 tgcgacgcgc cctaccgcca ggcgtgggtg gaaccgttcc acgagagcga cgcgccggtc
      961 cccctcccct cggacctcaa atgcagcctc caccgcctgg tccgcgaatt cggcagactg
     1021 accgggcgcg ccgccgagtt cgaggtcttc ctcacgcgca tggtgcacga acccgccttc
     1081 ctgaatatct gttgttccga actgcgcttc accgccgtgg aaaaacaact cctcctggaa
     1141 gcgacctgcc cgcttcaaca agccaaacgc ctggtcgaga ttctccagtt caacatcgac
     1201 gcgctcgttc gcaactccgc cccgaccaag aacgtcgggt cgtaatccgt tcgtgatccg
     1261 ttccctttca ccaaggagct gtccatgact cgatggttcg ccggattcgt cgtcgcttca
     1321 ttcgtgtcgg tgtcggcgtg ggtcgcggtc cccgcgagcg cggacggacc gttcagcgcg
     1381 atcaaccgcg cgccgaacga cgcgcacacc ccggtgatcg acgcgcccga cgcggtcaag
     1441 gcgggggagc cgtttacggt gacgatcacg gtgggcaaga cgccgcaccc ctcgcagccc
     1501 gaccacgcgg tgcagtggat cgccttgtac gccgacgagg tggaactggc ccgcgccacc
     1561 ctcaccccga cgctgacccg ccccaaggtg acgtttaccg tggtgctcga tcaatccgcc
     1621 acgttgcgcg ccctggaagc gcccaatcac tcggccgcgt gggaagcggt caagaagatc
     1681 acggtgtcca aagacgagaa gaaatagcca tggcgaacca acccaccgac ggaccgtccg
     1741 agggcgatct cgctccggac tttgcgctgg ccgccagcac cggccggacc attgcgttgc
     1801 acgagtttcg gggcaagcag gcggtggtgc tctatttcta tcccaaggac gacaccccgg
     1861 gctgcacgat cgaagcgtgc ggctttcgcg acacgttcgc ggcgtacggc aaggccggcg
     1921 ccgtgatcct gggcgtgagc ctcgacaacc tgaactcaca caagaaattc acggacaagt
     1981 tctcgctccc gtttcccctc ctgagcgacg aaacccagtc cgtgtccaaa gcctacggcg
     2041 tgtacaagct caagaagatg tacggcaagg aatacttggg gatcgaacgc acgaccttcc
     2101 tgatcgacaa acagggtcgg atcgccaaga tctacccgaa ggtcaaggtc gacgagcacc
     2161 accacgaggt gctcaaggac gtcaaggcgc tcaaataacg ggctgctgaa aaagctccag
     2221 ccgcaaggcg ccgcgagcac cgcaccggag cgtacgtggt atgtacgtga ggcgcggctg
     2281 cgcagcggca acgccgcgga tggacgtttt cagcagcctg cagcgggact tgacgatcgg
     2341 accaaccgct ccaacgcgac cagcacgtcc cgcccgatcg cgtccggcga gcagcgcgct
     2401 tcataggttc gccgcgcgcc ctcggccagg cgcgcgcgca acgcgcggtc tccggccaat
     2461 gtcgccagcg cgtcggccag cgccgcggga tcgtccggcg ggcacaacca cgcgtccacg
     2521 ccgtgcgtca gcagttcccg cgccgcgggc gtgtccgcgg tgatcaacgg ccgccccgcg
     2581 gccagcgcgt cgtacacctt tgacggaatc acccggcgcg ccttgtccgt ggcgccgaaa
     2641 atgcccaggc agatgtcggc cgcggccagc tcgcgttcgt acgcgtcgcc gttgatccag
     2701 cgatcgacca gctcaacgcg cgtcaggccc tctcgcccga ccaactcggc cgctcccgca
     2761 aactcctgac cgcggccgat cagacggaac cgaatgtcca gcccccgctg ctccacgcgt
     2821 tgggccgccg ccacgatcgt gcgcaccccg tgcagcggga taaacgtgcc cacgaacaac
     2881 acgcgaaccg atgcgccgga cggaccgatt gccgcgggcc ggccggaccg gtccgatcca
     2941 accggcacgc gaaggaacct cgcgtgatcc agtccgaact gctcgcggaa atacgcgatg
     3001 tgcgcgttgg tatcgagcag gaccagatcc gcggcgcggc acgccgagcg atcgatcccg
     3061 cgcaacagac gcgcggccaa cgatcgctcg gacgccaggc cgcgatcccg caccacggtg
     3121 tcgtgcagcg acaacagggc gttgagcgcc accggccgcc gggtgagcag gcgcgcgagg
     3181 aacacgtcga gatgcccgag atacccgacg atcaccacgt cggtgggcgg cccgctgatc
     3241 cagcgcgcga ccagccgaat ccacgccacg gccattcgtc ccgccgcccg cagccacgcg
     3301 agagggcctt gcgccgattt ccaccgttcc tccccgtcgt cggccgcgcc cgcgcgacac
     3361 tcccacaccg tggcgcccgc gcgccgcaac ccctcgatga gcacgcggtt gacggggtag
     3421 ccggcgccgg cggaataggt gccgaagtaa cacacgcgga gtggtcccga aacggcaaat
     3481 ccccccacgc cccccttttt caaagggggg aatgggggcg tttgcttctc ggagttcgtc
     3541 atcccccctt tagcaaaggg gggtaagggg ggatttgtct tgcgcgtcaa tgagccgagg
     3601 gctcatgccg attacgagtc cgagtggtcg tggtccgtca ggcgctcgct gtgctcgaag
     3661 cgcatctgcg cgatctgctc ggagacaagc cccatcagga agatgatcac cgcgttgatg
     3721 aagagcagcg cggacatgtt ggtgaaccgt cgggactcga tgaaggtgta gaggtagtac
     3781 cccagcccgg tcgcgaaaaa cgccgcgctc acgggcagga acaccttgat cggcgaatac
     3841 agcgtcgcga tcttgaacat gatgaccaga aagcgcgcgc cgtcccgcaa cggctggagc
     3901 ttgctctcgc ccttgcgccg ggcggtcacg atcggcacga acgtgacgct gtggcccgcg
     3961 cgaatcgtgg ccatggtgag ggtggtggga taggaaaacg cgttgggcaa cagatacacg
     4021 aagcgccgcg cgagcgacgc cttgatcgcg cggaagcccg acgtggtgtc caggatcggc
     4081 cggttcgtca ggtacgaggc gaacacgttg tagaagcggt tgccgagcca gcggtgcatg
     4141 tcgctctgcg acccggccgc gcgcgcgccc accgccatgt cgtagggccc gatctgggcc
     4201 accagccgcg gaacgtccgc ggggttgtgc tggccgtccc cgtccatcat caccaccacc
     4261 gacccgcgcg cggagcggat gccgctcttg atcgccgcgc cgttgcccat gcggtacgga
     4321 tggctcacca cccgcgctcc ggcatcgcgc gcgagttgcc cgctgccgtc ggtcgagccg
     4381 tcgtccacga ccaggacttc gtcctcgggc ccgagcacgt cccgggtgcg cttcacgagg
     4441 tccgtgatat tcatggcctc gttgtgcacg gggatgacga gcgacacgcg cgcgcggcga
     4501 acgggttcgg tcatacggcc tggaaggcta gacgatcagc agggaaaaag caatccatcg
     4561 cgttaagact ccgaaacgcg ggcctcatcc gcagccacgc gttggtacaa ttcatccatc
     4621 tgatcggcca tcctggacca atcgtagcgc gccctcaccg cgtcgagcgc gtcgcgctcc
     4681 agccgctggc gcagcgcggg atcgcgcagc agccggacca ccccggacgc gaacgcggcc
     4741 ggatcatccg ccaccacgag atgccggttc ggctcgagcc ccaggccctc gcaccccacc
     4801 gacgtggata ccaccgcggt gcccgcggcc atggactcca aaatcttgag ccgcgtcccc
     4861 ccgcccaccc gcagcggcac cacgctgacc gtggccgcgc gcagcggcga ccgcacgtcg
     4921 gggacgtagc cggccaactc cacgccggga acccgcgcgg ccgcccggcg caccgacgcg
     4981 gggaccggcg cgccgatcac caccagccgc gcgtccgaaa gatcttgcac gatcagcggc
     5041 cagacctcgt tcagaaagaa gagcagcccg tccacgttgg gatcgtggcg catccacccg
     5101 atgaagatca atgtgcgcgg atctttgacc ggcgccggcg actcggtgta gaacgcgacg
     5161 tccacgccgt tgggcaccac ggtcacgttc cggaccccgt cggccgcgag ccgcttcgcg
     5221 tcatccggcg agaccgccgc gaccgcgcga aacgccgagg cctgctcgcg ctcgtacgcg
     5281 cgcatcagac gcgcctcgcg ccgccagaac cagcgggcgg ggccggaagc cacggccgcg
     5341 cggcgctcga gcgggagcca cgacacgtcg tgctccacca gcacgccgcg ccgcgcgagg
     5401 attgcgcgat ccgccgcggc cacgtgctgc gccatgtgca cccactcgac ctgcaccacg
     5461 tcgaacgact cgcgttgcaa caagccgcga agcacggagg ccaattcggc gcttcgctta
     5521 ttgagcacta ccacgggcaa ttgcgcgacc cgcgcgcccg cggcgaacgc ccggcccagc
     5581 aaacccagca cgcccgggcg ccagcgcacg ccgatgaccc gctcgcacaa cgcgtccagt
     5641 tccgcgcggt ggcgcgcgaa ctcctcgtcc gagtccacca ggcacagcag ggtaatccga
     5701 tggcgcgttg ccagacgctt caggaggttg aacacgcgga tgcgcccgcc ggccttcaac
     5761 ggcagcgggc acgacatcga gacgagaagt atccgcaacg ggatcactcg ggcagggtgg
     5821 ccgcgatcaa cggattgtcc aactggtagt agaaccgcga ggcttgatcg tcgcaggccg
     5881 cgcgcaggtc ccgatcgtcg aacccccggc agagcgccgg gagcgccgcg cgggcgtggt
     5941 ggtccttttt catcaacgcg agcccggccg cgcaatagac gcgatccgcg ggctcggccg
     6001 cgccgcacac gcgccccgcg tcggcgggat ggcgcgtgag gtactgccgg gacacgtacc
     6061 cggccgcata gacgcatcct cgccgcgcct ggccgtccat tcgcccgcac gcgcgcacga
     6121 ggacgcgcgc gtcgtcggca tcctcggccc agggccacac gaaccgcaaa cacgcgccag
     6181 gcgcggacgc gtggaccgag caatagtcga ccccggcctt tgcgcgcgcc gcgaacgaca
     6241 tccgttcccg aatgatcccg gcccggatgt cgcggcgcgc ctccatgaac acgccggtct
     6301 cgcagtaaaa gcgcatctcc cccggctcga acacccggca ccacgccgcg gcccccggcg
     6361 cgtccgaggc gaccgccgcg aacgcgtgcc ccacgctgtg cgcgcaattg ccccggtaaa
     6421 atccgggaag aaccgtggag tcctcgcggc acagatcaga aatctcctgc gccacgtccg
     6481 gcgccacggc ggtcgctccc gcccgaccga gccgcttcga aaaatacgcc ctgaacgcgc
     6541 cgtgcacgca cccgaacgtg caccgatcgc tgcacgcgac catcgcctcg gtcagcgtcg
     6601 ccgcgcgctc ggcaatggcc gcgcccacgt cgtgcgacgt gccgtgacac gagggctcca
     6661 tcccctcgga ggcctcggcc gcgtgcgcgt cgatcccgtc gctcggcgcc gcggacccat
     6721 ggtcggccga aaccgttggg acagccggga gcgcgaaccc gatggtcaca gcgacagcgg
     6781 cgaccacggc gcgaagtcgc tcaagcaaag cggaccgtcc gcgagcgcct atttcgacag
     6841 ccaccccagc gcctccagct tccgaatcac tttgcccgcg ctctcctcca cgctttcgcg
     6901 atccgtgtgc aga
//
LOCUS       DBNGTT010000077         3075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1805441, whole genome shotgun sequence.
ACCESSION   DBNGTT010000077 DBNGTT010000000
VERSION     DBNGTT010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1805441"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="AC16BC_01795"
     CDS             <1..430
                     /locus_tag="AC16BC_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR3240375.1"
                     /translation="ARQAGLGLVCRNPFQSIVVRSVEILYACDEALRIIDQYQPPDAP
                     AIEVRPRAGTGYGCTEAPRGLLYHRYRIDEQGSILDAKIVPPTSQNQRTIEDDLRDVV
                     PRYLDLDKDKLTWRCEQTVRNYDPCISCATHFLTVNVTGT"
     gene            448..918
                     /locus_tag="AC16BC_01800"
     CDS             448..918
                     /locus_tag="AC16BC_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MamS/MamX family magnetosome protein"
                     /protein_id="HIR3240376.1"
                     /translation="MRKLLAGVMAVMVLGGASLALAGGGSKSTKSMKGMGNEGWGTGG
                     AYHRMYNPRTVESFAGDVVSVDYVSPMKGMSAGVHLMVRTDKETLSVHLGPAWYIDNQ
                     DVRVAPNDRLDIKGSRVAFNGQPAIIAAEVKKGDQVLALRDDAGVPAWSGWRRR"
     gene            968..1111
                     /locus_tag="AC16BC_01805"
     CDS             968..1111
                     /locus_tag="AC16BC_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240377.1"
                     /translation="MRCVLAVGLGVGWLGTATASFAIDAATLFSKKCAMCHTREGGRG
                     SHR"
     gene            1256..1549
                     /locus_tag="AC16BC_01810"
     CDS             1256..1549
                     /locus_tag="AC16BC_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240378.1"
                     /translation="MPADQERAQEQDKSQMQEQEQIYGSQMMTDQERTEYRAKMHAAK
                     TAEEREKIRKEHHERMKARAEERGMTLPDEPPMKGSGMGPGGGMGRGPGGGRP"
     gene            1809..3056
                     /locus_tag="AC16BC_01815"
     CDS             1809..3056
                     /locus_tag="AC16BC_01815"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3240379.1"
                     /translation="MARIVIIGASIGGLPAAYECRKAVGKAHTVTVVSNVDYFHFVPS
                     NPWVAVGWRTRKDISLGLAPVLKKKGIEFIHAAAERIDPARNAVVTARGDVPYDYLLI
                     ATGPKLNFGAVPGLGPSGHTQSICTVDHAEQTWGRYQGFLKDPGPIVVGAAQGASCFG
                     PAYEMAFILDADLRKRRIRRKVPITFVTAEPYAGHMGLAGVGKSRRLMEDEFAERSIT
                     PVTNASIREVQPGRVVLESGQEIPFRYAMFIPPFAGVDAVAGTSGLCNPKGFVNVDAY
                     QANPTYRNIYAVGVCVAIPPVEQTPVPTGAPKTGYMIESMVAAAVHNIKADIENDPKR
                     ETATWNAICLADMGDTGMAFVALPQMAPRDVTWAKKGKWVHLAKAGLEKYFLHKMKRG
                     VSEPFYERAILRAMGITKLRESA"
BASE COUNT          589 a          895 c         1136 g          455 t
ORIGIN      
        1 ggcgcggcag gccgggttgg gcctggtgtg ccgcaatccg ttccagagca tcgtggtgcg
       61 gagcgtggag atcctctacg cgtgcgacga ggcactgcgg atcatcgacc agtatcaacc
      121 gcccgacgcg ccggcaatcg aggtgcggcc gcgggccggc acgggatacg gttgcaccga
      181 agcgccgcgc gggctgctgt accaccggta ccggatcgac gagcagggat cgatcctgga
      241 cgcgaagatc gtgccgccca cgtcgcagaa ccagcgcacg atcgaggacg atctgcgcga
      301 cgtggtgccg cgttacctgg atctggacaa ggacaagctg acgtggcgct gcgagcagac
      361 cgtgcgcaat tacgacccgt gtatctcgtg cgccacgcat tttctgacgg tcaacgtgac
      421 cggaacctga caaccacgga ggacgcgatg cgaaagttgt tggcgggtgt gatggcggtc
      481 atggtgctgg gaggggcgtc gcttgccctg gccggcggcg gatccaagag cacgaagagc
      541 atgaagggga tggggaacga ggggtggggc acggggggcg cgtatcaccg gatgtacaac
      601 ccccggaccg tggagtcgtt tgccggcgac gtggtgagcg tggactacgt ctccccgatg
      661 aaggggatgt ccgccggggt tcacctcatg gtcaggaccg acaaggagac gctgtcggtc
      721 cacctcggcc cggcgtggta catcgacaat caggacgtca gggtcgcgcc caacgaccgc
      781 ctcgacatca aaggctcccg ggtcgcgttc aacggccagc ccgcgatcat cgcggccgag
      841 gtcaagaaag gcgaccaggt cctggcgttg cgggatgacg cgggcgtgcc ggcctggagc
      901 ggctggcgac ggaggtaggt cgggagggcc gacgggctcg atgcccgctt ccggaaggag
      961 gggaaacgtg agatgcgtgc tggcggtggg gctgggggtc gggtggctcg gtacggcgac
     1021 cgcgagtttc gcgatcgacg cggcgacgct cttcagcaaa aagtgcgcga tgtgccacac
     1081 acgggaaggc ggtcgaggga gtcaccggta gttcacaggg gaggttgacg cggtgaggga
     1141 gcgggcgcgt ctctcgccga gcaacctcaa aaccaggaaa ggagatccca tgcaacgaac
     1201 agggtggata gtctctgtcg tcacgagcgc actctttctc tcggctggag tcgcaatgcc
     1261 ggcagaccag gagcgcgcgc aagagcaaga caagtcgcaa atgcaagagc aggagcaaat
     1321 ctacggtagc cagatgatga cggatcagga acggaccgag tatcgggcca agatgcacgc
     1381 cgcaaaaacc gctgaagagc gagaaaaaat ccggaaggag caccacgagc ggatgaaagc
     1441 gcgcgccgag gagcgtggga tgacgcttcc cgacgaacct ccgatgaaag gaagcgggat
     1501 ggggcctgga ggcgggatgg ggcgtggtcc tggtggtggg cggccttgac gccggtcggc
     1561 gggtcgcaac cgcacgaagc ggccgaggga cgcgcacgcg tctctcggcc gcacagcccg
     1621 atcgtggaaa cccggttgac gcgacgcccc acacggtgtc ggcgatgtgg gaacatgaga
     1681 ccagctattc cctggcggtg tagccagagt acgcgcacgc ggtcatcttg tctgatccgg
     1741 gcgaggcgca ggacaatctc gtgtgggcga gcgtggcgta tcgatattga ggtcgaggag
     1801 ggatcggcat ggctcgaatc gtgatcatcg gggcgtcgat cggcgggttg cccgcggcgt
     1861 acgagtgccg caaggcggtg gggaaggcgc acacggtcac cgtggtgtcc aacgtggact
     1921 atttccactt cgtgccgtcc aacccctggg tggcggtggg atggcgcacg cgcaaggaca
     1981 tcagccttgg attggcgccc gtcctgaaga agaagggcat cgaattcatc cacgccgcgg
     2041 ccgagcggat cgacccggcc cgcaacgcgg tggtcacggc gcggggcgat gtgccgtacg
     2101 actacctcct catcgccacg ggcccgaagc tcaacttcgg cgcggtgccg gggttggggc
     2161 ccagcgggca cacgcagtcg atctgcaccg tggaccacgc ggagcagacc tggggccggt
     2221 accaggggtt cctgaaggat ccggggccga tcgtggtcgg cgcggcccag ggcgcgtcgt
     2281 gtttcggtcc cgcgtatgag atggcgttca ttctggacgc cgacctgcgc aagcggcgca
     2341 ttcggcggaa ggtccccatc accttcgtca ccgcggaacc gtacgcggga cacatgggcc
     2401 ttgccggcgt ggggaagtcc cgccggctga tggaggacga attcgccgaa cgctcgatca
     2461 cgccggtcac gaacgcgtcc atccgcgagg tccagccggg acgggtggtg ctcgagtcgg
     2521 gccaggagat tcccttccgg tacgcgatgt tcatcccgcc gttcgcgggc gtggacgcgg
     2581 tggcggggac gtcggggttg tgcaacccca aggggttcgt gaacgtggac gcgtaccagg
     2641 ccaatccgac ctatcggaac atctacgccg tgggcgtgtg cgtcgcgatc ccgccggtgg
     2701 agcaaacgcc ggtcccgacc ggcgcgccca agaccgggta catgatcgaa tccatggtcg
     2761 cggccgcggt gcacaacatc aaggccgaca tcgagaacga tcccaaacgc gagaccgcga
     2821 cctggaacgc gatttgtctg gcggacatgg gcgacaccgg catggcgttc gtcgcgctgc
     2881 cgcagatggc gccgcgggac gtgacgtggg cgaagaaggg caagtgggtg cacctcgcca
     2941 aagccgggct ggagaagtac ttcctgcaca agatgaagcg cggcgtgagc gagccgttct
     3001 acgagcgggc gatcctgagg gccatgggga tcaccaagct gcgtgaatcg gcgtagacaa
     3061 cgaacgtgac acagg
//
LOCUS       DBNGTT010000078         2843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1166950, whole genome shotgun sequence.
ACCESSION   DBNGTT010000078 DBNGTT010000000
VERSION     DBNGTT010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1166950"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2616
                     /locus_tag="AC16BC_01820"
     CDS             <1..2616
                     /locus_tag="AC16BC_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3240380.1"
                     /translation="GLAAEDAGRRLVEFGPNQVERVARRPLIVRFAKGLTHFFALILW
                     LAAGLAFIAEWSEPGQGMAALGAAVIAVILINGVFSFWQEYRAERAILALRDLLPHRV
                     RALRDRAVVEIPTADLAPGDVILLREGDDVPADCRVIEAFGLRVNNATVTGESLPKAR
                     DARPSAEDDPLRANNVLLAGTSVVSGEARAVVFATGMRTAFGAIAHLTQTAGETLSPL
                     QREIVRLSRVVAIVAVALGVVFFVIGRAIGLPFWANFIFAIGIIVANVPEGLLPTVTL
                     SLAMASQRMAKRRALVRRLASVEALGCATVICTDKTGTLTQNRMAVKRALVSGESLDA
                     DGLVARAGASVAARRVLEIARSCHDLKTVDRNGRSELAGDPMEIALVETAQRAGLAVD
                     RAPKIDEVPFDADRKRLSVLSRTPAGLILSTKGAPETVLPLCDRVREGDAVLPLSDER
                     RTRLLRGVEQMADRGLRVLAFADRGASEGEDRSTLERGLVLAGLVGLEDPPRPEVPAA
                     IRTCREAGIKVVMVTGDHPHTALAIAREIGLVRAASPTVITGDEIRRSSDTQLQLAVA
                     APEIVFARVTADQKMRIVAAFQARREIVAVTGDGVNDAPALKKADIGIAMGLSGTDVA
                     REAADMVLLDDNFASIVAAVEEGRAVFDNIRKFLTYILTSNIPEVVPYLAFVLFKIPL
                     PLTVIQILAVDLGTDMLPALALGAEPPNHAVMRRPPRARAERLLDRGLLLRAYVFLGG
                     LEAVAAMSAFFFVLTAGGWDYGDRLAGTDPLYLRATTACLSAIIVMQVVNVFACRDHR
                     ASAFAFGLFSNPLILAGIGAELALILLIDYTPWGHALFGTAPIPGTVWLFVVPFALGM
                     LALEEGRKWLVRRRR"
     gene            complement(2632..>2843)
                     /locus_tag="AC16BC_01825"
     CDS             complement(2632..>2843)
                     /locus_tag="AC16BC_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB family protein"
                     /protein_id="HIR3240381.1"
                     /translation="PKPGMVLLKIDVDAKGRVAAVEVVESTNVLLESAARQAALQWSF
                     EPAMRHGFPVPATIVVPVEFTQGER"
BASE COUNT          384 a          943 c         1034 g          482 t
ORIGIN      
        1 gggctcgccg cggaggacgc gggccgtcgg ctcgtcgagt tcggccccaa tcaagtcgaa
       61 cgggtggcgc ggcgccccct gatcgtccgg ttcgccaagg gcctcaccca ctttttcgcg
      121 ctgattctct ggctggccgc tggcctcgcc ttcatcgccg aatggagcga gcccggccag
      181 ggtatggccg cgttgggcgc ggccgtgatc gcggtgatcc tgatcaacgg cgtgttttcc
      241 ttctggcagg agtaccgcgc ggagcgcgcg attctcgcgc tgagggatct cttgccccat
      301 cgggtccgcg cgctgcgcga tcgggcggtg gtcgagattc ccaccgcgga tctggcgccc
      361 ggcgacgtga tcctgctccg ggagggcgac gacgtgccgg cggactgccg ggtgatcgag
      421 gcgttcggcc tgcgggtcaa taacgccacg gtgaccgggg aatccctgcc caaggcgcgc
      481 gacgcccgtc ccagcgcgga ggacgacccg ctccgcgcga acaacgtgct gctcgcgggc
      541 acgtccgtgg tttcgggcga ggcgcgggcg gtggtgttcg ccaccggcat gcgcaccgcg
      601 ttcggcgcca tcgcgcacct cacgcagacc gcgggcgaga cgctctcgcc cctccagcgg
      661 gagatcgtcc ggctcagccg ggtcgtggcg atcgtcgcgg tggcgctcgg cgtggtcttt
      721 ttcgtgatcg gacgcgcgat cgggctgccg ttctgggcca acttcatctt cgcgatcggg
      781 atcatcgtgg ccaacgtgcc cgaggggttg ttgcccacgg tcacgttgtc gctcgccatg
      841 gcctcgcaac ggatggccaa acgccgggcc ctggtgagac ggctcgcctc ggtcgaggcg
      901 ctgggctgcg ccaccgtgat ctgcacggac aagaccggca ccctcacgca gaaccggatg
      961 gccgtgaagc gcgcgctcgt gtccggcgaa tccctggacg cggacggtct cgtggcgcgc
     1021 gcgggggcgt ccgtcgcggc gcgacgggtc ctggagatcg cgaggtcctg tcacgatctc
     1081 aagaccgtgg atcgcaacgg ccgatcggag ctcgcgggcg atcccatgga gatcgcgctg
     1141 gtggagacgg cgcaacgggc cggattggcc gtggaccgcg cgccgaaaat cgacgaagtg
     1201 ccgttcgacg cggatcggaa gcgcctctcc gtgttgtccc ggacgccggc gggcctgatc
     1261 ctctcgacca agggggcgcc ggaaaccgtg ctgcccttgt gcgatcgggt ccgtgagggc
     1321 gacgcggtgt tgcccttgag cgacgagcga cggacgcggc tcctccgcgg cgtggagcag
     1381 atggccgacc gcgggttgcg ggtgctggcg ttcgcggatc ggggcgcgtc ggagggcgaa
     1441 gaccggtcga ccctggaacg ggggctcgtg ctcgccggac tggtgggcct ggaggatccg
     1501 ccgcgtcccg aggtgcccgc cgcgatccgc acgtgccgcg aggccggcat caaggtggtg
     1561 atggtcaccg gggatcatcc ccataccgcg ctcgcgatcg cgcgcgagat cgggttggtg
     1621 cgcgccgcct cgccgacggt gatcaccggc gacgaaatcc ggcggtcgtc cgacacgcag
     1681 cttcaactcg cggtggccgc gccggagatc gtctttgccc gggtcaccgc ggatcagaaa
     1741 atgcgcatcg tggccgcgtt ccaggcgcgc agggagatcg tggcggtcac gggcgacggg
     1801 gtgaacgacg cgcccgcgct gaagaaggcg gacatcggca tcgccatggg cctgtccggc
     1861 accgacgtgg cgcgcgaggc cgcggacatg gtgttgttgg acgacaattt cgcgagcatc
     1921 gtggccgcgg tcgaagaagg ccgcgcggtg ttcgacaaca tccgcaagtt cctcacgtac
     1981 attctcacgt cgaacattcc ggaagtcgtg ccgtacctcg cgttcgtgtt gttcaagatt
     2041 ccgttgccgc tcaccgtgat ccagatcctg gccgtggacc tggggaccga catgctgccc
     2101 gcgctcgcgc tgggcgcgga gccgcccaac cacgcggtga tgcggcgccc gccgcgcgcg
     2161 cgcgccgaac gcctgctcga tcgggggctg ctcctgcgcg catacgtgtt cctcggcggg
     2221 ctggaagcgg tcgcggccat gagcgcgttc ttcttcgtcc tgaccgcggg aggctgggac
     2281 tacggagacc gtctggccgg gacggatccc ctctatcttc gggccaccac ggcgtgtttg
     2341 tccgcgatca tcgtgatgca ggtggtgaac gtgttcgcct gccgcgacca tcgcgcgtcg
     2401 gcgttcgcgt tcggcctctt cagcaatccc ctgattctgg cgggaatcgg cgcggaactc
     2461 gcgttgatcc tgctgatcga ctacaccccc tggggacacg cgctgttcgg caccgccccg
     2521 atcccgggca ctgtctggtt attcgtggtc ccctttgccc tcggcatgct ggcgttggag
     2581 gaggggagga agtggctggt gcggcggcga cggtaagcgc ggggtccgga gtcaccgttc
     2641 gccctgcgta aactcgaccg gcaccacgat ggtggccggc accgggaacc cgtgccgcat
     2701 ggcgggctcg aacgaccact gcagcgcggc ctgtcgcgcc gcggattcga ggagcacgtt
     2761 tgtggattcg acgacctcca cggccgcgac ccggcctttg gcgtccacgt cgatcttgag
     2821 caggaccatt ccgggtttgg ggc
//
LOCUS       DBNGTT010000079         4124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_17621, whole genome shotgun sequence.
ACCESSION   DBNGTT010000079 DBNGTT010000000
VERSION     DBNGTT010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_17621"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC16BC_01830"
     CDS             complement(<1..484)
                     /locus_tag="AC16BC_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR3240382.1"
                     /translation="MPEIPDVNAIVAYLRTQILGATITDASLPLAWMLRNATADPAEQ
                     ALRGETIDAVDRRGKHVLFSLRRTSLVINPMLVGRFYYQLPEAKPPRQTVMDLRLSTG
                     KALRYADERQMGRLYLVPDRDYAKIPGFLEQGPEPLGEDFTFERFLDRLKGRYGEIKG
                     L"
     gene            967..3210
                     /locus_tag="AC16BC_01835"
     CDS             967..3210
                     /locus_tag="AC16BC_01835"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR3240383.1"
                     /translation="MSLSAIQAVYKKIAETAPKARKKFGRPLTLTEKILVSHADDFDS
                     QVWDRGKAQLLLRPDRVAMQDATAQMALLQFMQAGKKRVAVPSTVHCDHLIRAQSGAK
                     ADIERAIEENKEVYNFLRSAAAKYGIGFWKPGAGIIHQVVLENYGFPGGLIIGTDSHT
                     PNGGGLGMLAIGVGGADAGEVMAGLPWEVLHPKLIGVRLTGQLQGWASAKDVILYLCG
                     LLTVKGGTNKIIEYFGPGAATISATGKGTITNMGAELGATTSIFPFDDKMVAYLNITE
                     RADIAKLAQANRELITADPEVLQSPEKYYDQIVEINLSELEPHVVGPHTPDLARPVSK
                     LPAEAKEKGYPMTLTAALIGSCTNSSYEDISRAAHIARQGLKAGLKAKAHFLVTPGSE
                     RIYHTMQRDGFMKTFEDMGGTVLANACGPCIGQWKRDDIKQGEKNSIISSFNRNFPAR
                     NDGNAETLSFLSSPEVVTAMAFAGTLDFNPVSGTLTTTDGTKIKFEPPVGEELPAKGF
                     AKGEEGFIAPAENGDAVKIEVAPGSERLALLEPFPKWDGKDFAKLPILLKAKGKCTTD
                     HISPAGPWLKYRGHLDKISDNMFLGATNAFTGQAGKVNNVLTGDTNKAPSDVARAYKA
                     QGIGSIVIGDENYGEGSSREHAAMSPRFLGVKVVITKSFARIHETNLKKQGILPLTFA
                     NAADYDKFEEKDRVSITGLSNLAPGKPVDVTIHRASGDVKIQANHSMTAQQIGWFKAG
                     SALNALG"
     gene            3248..3484
                     /locus_tag="AC16BC_01840"
     CDS             3248..3484
                     /locus_tag="AC16BC_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HIR3240384.1"
                     /translation="MPTVIRSGPYRFFFYAGDRDEPPHVHIERDDKVAKFWLDPIRLQ
                     SSGGFSRVDIVWIERLVRTNRTKLIEAWNEYFGR"
     gene            3465..3812
                     /locus_tag="AC16BC_01845"
     CDS             3465..3812
                     /locus_tag="AC16BC_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR3240385.1"
                     /translation="MSISAVEIEVPLAQKVTVTDDTLCVDLSDGRSISVPLAWFPRLQ
                     HASSTERKRWRLIGKGHGIHWGDIDEDISVEGLQAGRPSGESQLSFKRWLETRTPRRP
                     KKRSNGHGKNARR"
BASE COUNT          872 a         1297 c         1291 g          664 t
ORIGIN      
        1 gcaggccttt gatttcgccg taccggccct tgaggcgatc cagaaaccgc tcgaacgtga
       61 agtcttctcc cagcggctcc gggccttgtt cgagaaaccc cgggatcttg gcgtagtcgc
      121 gatccgggac caggtagagc cgtcccatct gacgctcgtc cgcgtagcgc aaggccttgc
      181 ccgtggacag ccgcaggtcc atcacggtct gccgcggcgg cttcgcctcc gggagctggt
      241 agtagaagcg gcctaccagc atgggattga taaccagact ggtccgccgc agcgagaaca
      301 ggacgtgttt gccgcgccgg tcaacggcgt cgatggtctc gccgcgcagc gcctgctcgg
      361 cggggtcggc ggtcgcattg cgcaacatcc acgcgagcgg gagcgacgcg tcggtgatcg
      421 tggcgccgag gatctgcgtg cgcagatacg ccacgatggc attcacatcg ggaatctcgg
      481 gcacgcccga gtctaccgcg tcgctcccgc gggatcaacg cgcgttcggt tccggatggc
      541 aggcatcgtg tgcaggccgc agttcgtcat gatgagggtt tcaagttccg tcggcatcgt
      601 cgctcctccc cgcacggtac acaccagcca gctgaaattg agtatggcgg cggtcgtgga
      661 cattgccaaa acgcatggtt attcaggctg ctcaaaaagc tccagatgca aggccgcagg
      721 gaggagggaa cccggagcgt actgtgtcgt acgtgaggat tcccgacgac cgagcacgca
      781 gcagatggac tttttcagca gcctctgtgc cgcgcccggg gcgcggcaca gggaccggta
      841 ggcctacgcc ctctggtccg ttgacagggt tgtcgagcct tccttagaat gagccgctcc
      901 ggggatgttt ccccgtcacg tccgacgttc actctcagat cgatatttat ttaaaggagg
      961 actcgtatgt cgctgtccgc tattcaggcc gtctacaaaa agatcgcgga aaccgcgccc
     1021 aaggcccgca agaaattcgg ccgcccgttg acgttgaccg aaaagattct ggtctcgcac
     1081 gcggatgatt tcgacagcca ggtgtgggac cgcggcaagg cgcagttgct gttgcggccg
     1141 gatcgcgtgg ccatgcagga cgcgaccgcg caaatggcgt tgctgcagtt catgcaggcc
     1201 ggcaagaagc gcgtggccgt gcccagcacc gtgcactgcg accatttgat ccgcgcgcag
     1261 tccggggcca aggcggacat cgaacgcgcg atcgaagaaa acaaagaggt ctacaacttc
     1321 ctgcgttcgg ccgcggccaa atacgggatc gggttctgga agcccggcgc cggcatcatc
     1381 caccaggtgg tcctggaaaa ctacgggttc cccggcggcc tgatcatcgg caccgactcg
     1441 cacaccccca acggcggcgg gctcggcatg ctggccatcg gggtgggcgg cgcggacgcg
     1501 ggcgaagtca tggctggcct gccctgggaa gtgctccatc cgaaactgat cggcgtgcga
     1561 ctcaccggcc aattgcaggg ctgggcctcg gccaaggacg tgatcctgta cctgtgcggc
     1621 ctgctcacgg tcaaaggcgg caccaacaag atcatcgagt acttcggccc cggcgccgcc
     1681 acgatcagcg caaccggtaa gggcaccatc accaacatgg gcgcggagct gggcgcgacc
     1741 acctcgatct tcccgttcga cgacaagatg gtggcgtacc tgaacatcac tgagcgcgcg
     1801 gacatcgcga aactggcgca ggccaaccgc gaattgatca ccgcggaccc cgaagtgctg
     1861 caatcgcccg agaagtacta cgaccagatc gtggagatca atctgtcgga acttgagccg
     1921 cacgtggtgg gtccgcacac gcccgacctg gcgcgcccgg tctccaagct cccggccgag
     1981 gccaaggaga agggctaccc catgaccttg accgcagccc tcatcgggag ctgcaccaac
     2041 tcctcgtacg aagacatcag ccgcgccgcg cacatcgcgc gccagggctt gaaagccggc
     2101 ctcaaagcca aggcgcactt tctggtcacg ccgggctcgg agcgcatcta ccacaccatg
     2161 cagcgcgacg ggtttatgaa aacgttcgag gacatgggcg ggacggtcct ggcaaacgcg
     2221 tgcggcccgt gcatcggcca gtggaagcgc gacgacatca aacagggcga gaagaactcg
     2281 atcatcagct cgttcaaccg caacttcccg gcgcgcaacg acggcaacgc agaaaccctg
     2341 tcgttcttgt cgagccccga ggtcgtgact gcgatggcgt tcgcgggaac cttggacttc
     2401 aaccccgtga gcggcacgct gacgaccacg gacggcacca agatcaaatt cgagcctccg
     2461 gtcggcgaag aactgccggc caagggattt gccaagggcg aagaaggctt catcgccccg
     2521 gctgagaacg gcgacgcggt aaaaatcgaa gtagcccccg gcagcgagcg cctggccctg
     2581 ctggaaccgt tcccgaaatg ggacggcaaa gacttcgcca aattgccgat cctcttgaag
     2641 gccaagggca agtgcaccac cgaccacatc tccccggccg gcccctggct caagtaccgc
     2701 ggccacctgg acaagatcag cgacaacatg ttcctgggcg ccaccaacgc gttcaccgga
     2761 caagccggca aggtcaacaa cgtgctgacc ggcgacacca acaaagcccc cagcgacgtg
     2821 gcccgcgcgt acaaggcgca aggcatcggc tcgatcgtga tcggcgacga aaactacggc
     2881 gagggcagca gccgcgaaca tgccgccatg tccccgcgct tcctgggcgt gaaagtcgtg
     2941 atcaccaaga gcttcgcccg catccacgaa acgaacctga aaaagcaggg catcctgccc
     3001 ctgaccttcg cgaatgccgc ggactacgac aagttcgaag aaaaagaccg cgtgagcatc
     3061 accggcctct caaacctcgc gcccggcaag ccggtggatg taacgatcca cagggccagc
     3121 ggggacgtga agatccaggc caaccattca atgaccgcgc agcagatcgg gtggttcaag
     3181 gcggggtcgg cgctgaacgc gctggggtaa gccgtttgcg gacgtcgaat gtccacggaa
     3241 ccggtagatg cccaccgtca taaggtccgg gccctaccgg tttttctttt atgcgggcga
     3301 ccgcgatgaa cctccgcacg ttcatatcga gcgagacgac aaggttgcca aattctggct
     3361 cgatccgata cgcttacaga gtagcggcgg gttctctcgg gtggacatcg tgtggatcga
     3421 acgactcgtg aggaccaacc gaacgaaact gatcgaggcg tggaatgagt atttcggccg
     3481 ttgaaatcga agtccccttg gcccagaagg tcacggtcac cgacgacacg ctctgcgttg
     3541 acctgagtga cgggcgtagc atctcagtcc cgttggcctg gtttccccga ttgcagcacg
     3601 cgtcatcgac cgaacgcaaa cgctggcgcc tgatcgggaa agggcacggc atccactggg
     3661 gagacattga tgaagacatc agcgtcgaag ggctgcaggc aggtcggcct tccggcgaga
     3721 gtcaactctc tttcaagcgt tggctggaga cccgcacgcc tcgtcgcccc aagaagcgtt
     3781 cgaacggaca cgggaaaaac gcacggcggt gacgcgggtg cgtcgcaatc actaaagtca
     3841 acaagtcaag tctgattaga tgaaaaaggg ctgcgcctcc tggcgcgaag ggtctcgaag
     3901 accacggccg gtataatccg gcccacgagc tgaccacggg ggtcgctcac gccacaggag
     3961 ggcagccatg gggcactcta tcggaatcga tttgcacaag gcaacctcgt acgtgaccac
     4021 agtggacgag acgggacgga ttctggacca acgcaacctg cgcaatgagc cggagacgct
     4081 gcgcgcgtac tttgcggcgc agcccgcgga tgcgcggctg gtgg
//
LOCUS       DBNGTT010000080         2478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3211583, whole genome shotgun sequence.
ACCESSION   DBNGTT010000080 DBNGTT010000000
VERSION     DBNGTT010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3211583"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="AC16BC_01850"
     CDS             <1..1324
                     /locus_tag="AC16BC_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3240386.1"
                     /translation="TWKVDNTLGGTTVIGTIDSSGLYLAPVKIPTASTVVGAGDVAGV
                     TVSASGATNSAAVTLVSRFEAAAPLPVSTCTPCSELPSAVVAADLDNDTYADIITANP
                     GNGTVSVLLATGAGGFSTPVSSPVANGDSEPDALVVSSFTSDANLDVVVADGSFVSPA
                     VRTLPGTGTATFGPEQGRSLPAGSDPLSMASGRFNANDVYPDIVVANFLANTIQVLLG
                     DGNGGFGAATSILGGTFDSPLDVAAGDFNHDGVDDLAIASNGFLNPQASLNKLVIAIN
                     DGSGGFLAPEYYDIPGSPSAVALADLNGDTYLDALVTDGGGDTLTIYLNRAGAAGKIL
                     NTSWSDSLATGRNPLAVAAGDFNQDGAQDAVVVNNLSGTITTYFGRNNGTLTPSETYR
                     VRETDPGSAEPLAVAVSDFDRDGWPDLVVANSGDDSVTVFRNRGAATP"
     gene            1410..2390
                     /locus_tag="AC16BC_01855"
     CDS             1410..2390
                     /locus_tag="AC16BC_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3240387.1"
                     /translation="MPTVLVTGNAGFIGSHLTERLLALGHSVVGFDNFDPFYDPAIKR
                     KNLAIVTSHPHFTFVEGDLRRREQVDALFAGCRIDRIFHGAARAGVRPSLKDPLLYEE
                     VNVRGTLHLLEAATRHRVDNFVFASSSSVYGEQKKVPFSETDPVDHPISPYAATKKAC
                     ELMCFTYHHLYSLPTTCLRFFTVYGPRQRPEMAIHAFTRAIERGEPLKLFGDGSARRD
                     FTYIDDIIAGVTAALDRPHPYEVINLGESQTIALRDLIARLEQLLGKRANIQALPAEP
                     GDVPITYADVSKARALLDYNPSTPVEDGLARFVAWYRTTAGSASRKDESR"
     gene            2387..>2478
                     /locus_tag="AC16BC_01860"
     CDS             2387..>2478
                     /locus_tag="AC16BC_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018581813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3240388.1"
                     /translation="MRILVTGGAGFIGSHLVDRLIEEGHQVAVV"
BASE COUNT          461 a          738 c          807 g          472 t
ORIGIN      
        1 gacatggaag gtcgacaaca cgctaggcgg caccacggtc atcggaacga ttgattcgag
       61 cgggctctac ctcgcgccag tgaaaattcc caccgcgagc accgttgtgg gagccggcga
      121 cgtggcaggc gtgacggtta gtgcgtccgg cgcaacgaac tccgcggccg tgacccttgt
      181 gagccggttc gaagccgctg ctccactacc ggtgagcacc tgtaccccgt gttccgaact
      241 gcccagcgcg gtggtcgctg ccgatctgga taatgacacc tatgccgaca tcatcacggc
      301 caacccggga aacggaacgg tgtccgttct cctggcgact ggcgcaggag ggttttcaac
      361 tccagtgagc agcccggtgg cgaatgggga tagtgagccg gacgcattag tcgtatccag
      421 tttcacgtcg gatgccaatc tggatgtcgt cgtggccgat ggaagttttg tctcgcctgc
      481 cgtccgtacc cttccgggaa ccggaaccgc aacctttggg ccggagcagg gaaggagcct
      541 gccggccggc agtgatccat tatccatggc gtcgggacga ttcaatgcga acgacgtcta
      601 tcctgacatc gtggtcgcga actttctggc gaacacgatc caagttttgc ttggagacgg
      661 taacggggga ttcggagccg cgacatctat tttgggtgga acctttgact cgccgctcga
      721 cgtggcggcg ggtgatttca accacgacgg ggtcgacgat ctcgcaattg cgagtaacgg
      781 cttcctgaat cctcaggcga gcttgaacaa gttggtgatc gccattaacg acggaagcgg
      841 cggttttctg gcgcccgagt actacgatat cccgggatcc cccagcgcgg tggctcttgc
      901 tgacttgaat ggggacacat atttggacgc gctggtcact gatggagggg gtgacacgct
      961 cacgatctat ctcaatcgcg caggggcggc gggaaagatc ctcaatacgt cgtggtcgga
     1021 cagtcttgca accggtcgta accccttagc ggtcgctgcc ggggatttca accaagatgg
     1081 agcgcaagac gctgtggtgg tcaacaacct gtcaggcaca attaccacct actttggccg
     1141 caataatggg acgctcacgc cctcggaaac gtacagggtg cgggaaaccg atccggggag
     1201 cgcggagcct ctggccgtgg cagtaagcga tttcgaccgc gacggctggc cggatctggt
     1261 ggtggccaat agcggcgacg actcggtgac cgtctttcgc aaccgcggcg cggcgacgcc
     1321 ctgatccgtt tgacatcccc tggccgtcct gatagggtac gaccggcgcg cggggcggcc
     1381 cgcgcagact gattcggtat acggaggaca tgcccaccgt tctcgtcacg ggcaacgcgg
     1441 ggttcatcgg atcgcacctg accgagcgtc tgttggcgtt ggggcacagc gtggtcgggt
     1501 tcgacaactt cgatccgttc tacgaccccg cgatcaagcg caagaacctc gcgatcgtga
     1561 cgtcgcaccc ccacttcacg ttcgtggaag gggatctccg ccgccgcgag caggtggacg
     1621 cgctgtttgc cgggtgccgc atcgaccgca tttttcacgg cgcggcgcgc gcgggcgtcc
     1681 ggccgtcgct caaagatccc ctgttgtacg aggaggtgaa cgtgcgcggg acgctccatt
     1741 tattggaagc cgcgacgcgc caccgcgtgg acaacttcgt gttcgcgtcg tcctcctcgg
     1801 tctacggcga gcagaagaaa gtgccgttct cggagaccga tcccgtggat catccgattt
     1861 ccccgtacgc cgcgaccaag aaggcgtgtg agctgatgtg cttcacctat caccatttat
     1921 atagcctgcc cacgacgtgc ctgcggtttt tcacggtgta cggcccgcgg cagcggccgg
     1981 agatggccat tcacgcgttc acgcgggcca tcgaacgagg cgaacccctc aaattgttcg
     2041 gggacggcag cgcgcggcgc gactttacgt atatcgacga catcatcgcg ggggtgacgg
     2101 ccgcgctgga ccggccgcac ccgtacgaag tgatcaactt gggtgagtcg caaaccatcg
     2161 cgttgcggga cttgatcgcg cgattggaac aattactcgg caaacgcgcg aacatccagg
     2221 cgctgccggc cgagcccggc gacgtgccca tcacgtacgc ggacgtgtcc aaggccaggg
     2281 cgttgctgga ctacaacccg tcaacgcccg ttgaggacgg gttggcgcgt tttgtcgcgt
     2341 ggtatcgaac gactgcgggc tcggcgtcac gcaaggacga gtcacgatga ggattctggt
     2401 cacgggcggg gcggggttca tcgggtcgca tctggtcgac cggctgatcg aggaaggcca
     2461 ccaggtggcg gtcgtgga
//
LOCUS       DBNGTT010000081         5274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1295156, whole genome shotgun sequence.
ACCESSION   DBNGTT010000081 DBNGTT010000000
VERSION     DBNGTT010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1295156"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="AC16BC_01865"
     CDS             <1..660
                     /locus_tag="AC16BC_01865"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR3240389.1"
                     /translation="VYRIIMLVIAVTFVITMGWWGFSDDDRQVVARVGHRTITMDEYE
                     RAYRNYERAYRDALKEQFTPEFAKQLDLKHVVVNALVERQLWLDTAHSLGLTVTPGEL
                     AKSIAAISAFHNERTGQFDADAYRRVLAANRLSPEAFESAQREDLLITKVKALVSEST
                     ALSESEWDEARTRQAAQNPGPTSDTMLASPLPQKQERALRAYMTQVRRHADVEIHDNM
                     L"
     gene            complement(673..1239)
                     /locus_tag="AC16BC_01870"
     CDS             complement(673..1239)
                     /locus_tag="AC16BC_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240390.1"
                     /translation="MNPSSRIGPDHPLRGFFSVLTEKSFYEQLGWPDHRVIRYVANLL
                     ADFADVNRVYLFDDESGQRRVRLADMLRDAEAGPSGLPEREAHRHIGDFTLFMMGLFP
                     EHLMRLKTQVIAANADTLLDYVKIGKRSYRIVAEIPDEEGADADAVLFRKLSEHFELC
                     VAGLGWVREGLRVHRPMSFDRFRERFLN"
     gene            complement(1337..2251)
                     /locus_tag="AC16BC_01875"
     CDS             complement(1337..2251)
                     /locus_tag="AC16BC_01875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3240391.1"
                     /translation="MSPPPNADRLGKVVPLLVGATAFLASVAWAAGDVPAPTKTVKQG
                     DVFALAIDPGVMDGASDTSGLVARFGDHEWPLFRTSGGVGVLIGVDMETPPGVREFVV
                     ERKSGENPGVMTRGTVTVEKGDFGIQTLSLPDRQVDLDPQTLRRVEEDQRVMLASMQE
                     VTPRLWDGAFVLPNEGPLQHTFGRRRVINGQPRRPHSGEDITAPAGATVVAINHGVVR
                     LVEEQFFSGKSIVVDHGTGLYSMYFHLSETDVKVGDHVAKNQVIGKVGATGRASGPHL
                     HWGVRFNGARVNPLSLNAALNGLLTAAK"
     gene            complement(2229..3605)
                     /gene="pafA"
                     /locus_tag="AC16BC_01880"
     CDS             complement(2229..3605)
                     /gene="pafA"
                     /locus_tag="AC16BC_01880"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018219798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HIR3240392.1"
                     /translation="MPPRIFGLESEYGLIFSPNGRVYLPMEKILGYIFEGLIPNSWPS
                     NAFLCNGARFYQDTGCHPEYSTPECDRLHDLVVHDKAGERLLEACLPMAEKRLREEGL
                     VGEIYIFKNNTDSMGNTYGCHENYLMRRDLDFWKVTEQIIPFFVTRQIFAGAGKVLKV
                     SGRTHFFLSQRAQHIHEKTSSSTTSSRSIINTRDEPHADAEKYRRLHIIVGDSNMSEL
                     STYLKVGTIALVLSMIEEGYTVKGMELEDPVKAIRDISRDPQLKKRVRLEDGRELTAL
                     EIQMAYWERAQAYVAAMGSSADETTKDLVAYWGSVLERLAEDPMQLATELDWVIKKNL
                     MESFIDRKKCGWDDPRVALMDLQYHDVKRDRGLYYLLERQGLVDRVTTESEIQYAMAE
                     PPQTTRAKVRGDFIKFAKDKNRSYTVDWTYLKLNGYWEETILCMDPFASSNKRVEELI
                     AGVAASQR"
     gene            complement(3614..4291)
                     /gene="prcA"
                     /locus_tag="AC16BC_01885"
     CDS             complement(3614..4291)
                     /gene="prcA"
                     /locus_tag="AC16BC_01885"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019773 - proteasome core complex,
                     alpha-subunit complex [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HIR3240393.1"
                     /translation="MALPYYVSPELIMQDKAEYAKKGIARGRSTVVMEYEHGLLLMAD
                     NPNVSLNKISEIYDHIAFAGAGKYSEFENLRKAGIRHADMKGYLYSREDVTTKSLANA
                     YSQTLGTVFSQEIKPLEVEILVIGLGEGAATEIYRISFDGSIFDERLVAVIGGRSEAI
                     LSYLKQHVNGPRPLAAALALGLEALESVGHQGLRLSQLEVAVLDRRRTGRKFRRVPAA
                     ELNALTG"
     gene            complement(4292..5110)
                     /gene="prcB"
                     /locus_tag="AC16BC_01890"
     CDS             complement(4292..5110)
                     /gene="prcB"
                     /locus_tag="AC16BC_01890"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HIR3240394.1"
                     /translation="MVRDPGWGETNGLGSSFYDLLITQYSYLQPHRPADATPSGGAGF
                     SLAASVPHGTTVLALRYADGVVVAGDRRATEGYQISGRRIEKVFKVDEWSAMAISGAA
                     GPCLEMARLFQTELEHYEKIEGTTLSVDGKANKLAQMVKGNLPAAFQGLVVIPLFAGF
                     DHRKSVGRIFKYDVAGGRYEEADYHATGSGGKDARTTIKQYFKAGLNERDALNLSLEA
                     LFNAAEEDVATGSPDPTRGIFPSVKLVTAQGITDVDVDRIAPVYAAFLEKRRES"
     gene            complement(5114..>5274)
                     /locus_tag="AC16BC_01895"
     CDS             complement(5114..>5274)
                     /locus_tag="AC16BC_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031386 - protein tag activity [Evidence
                     IEA];
                     GO_function: GO:0070628 - proteasome binding [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HIR3240395.1"
                     /translation="EGGGQDGEGAQKLAEKGAELKEEMDNLLDEIDAVLEENAEEFVK
                     NYVQKGGE"
BASE COUNT         1026 a         1725 c         1481 g         1042 t
ORIGIN      
        1 gtctaccgga tcatcatgct ggtcatcgca gtgacgttcg tcatcacgat gggatggtgg
       61 ggatttagcg acgacgaccg ccaggtcgtc gcccgcgtcg gacatcgcac gattaccatg
      121 gacgaatacg agcgcgccta ccgcaactac gaacgggcct accgggatgc gctgaaagag
      181 caattcaccc ccgagttcgc caagcagctt gatctgaaac acgtggtagt caacgcgctg
      241 gtcgaacgcc agctgtggct cgacaccgca cacagcctcg gcctgaccgt cacccccggc
      301 gaactggcga agtccatcgc cgccatcagc gcgtttcaca acgaacgcac cggacaattc
      361 gacgccgacg cttatcgtcg ggtcctggcc gccaaccgtc tcagtcccga agccttcgag
      421 tccgcccaac gggaagacct cctgatcacg aaggtcaaag ccctcgtgag cgagtccacc
      481 gccttgtcgg aatccgaatg ggatgaggcg cgtacgcgtc aggccgcgca aaatccgggt
      541 ccgacctccg acacgatgct ggcgtcgccc ttgccgcaaa aacaagagcg cgcgcttcgc
      601 gcctacatga cccaggttcg ccgccatgcc gacgtggaga tccatgacaa catgctgtaa
      661 ccgcgttata ggttaattga ggaaccgctc acggaaccga tcaaacgaca tcgggcggtg
      721 aactctcaag ccttctctga cccaccccag ccccgccacg cacagttcaa agtgctcgga
      781 cagcttccga aataacaccg cgtcagcgtc agccccctct tcatcgggaa tctccgccac
      841 gatacgatac gaccgtttgc cgattttcac gtaatcgagc agcgtgtcgg cgttggccgc
      901 gatgacctgg gttttcaggc gcatcaggtg ttcgggaaat agccccatca tgaagagcgt
      961 gaagtcgccg atgtgacgat gagcttcccg ctcgggcaga cccgacggtc ccgcttcggc
     1021 gtcgcgcagc atgtccgcca gccgaacccg gcgttgcccg ctctcgtcat caaacaagta
     1081 cacccgattc acgtccgcga aatccgcgag cagattcgcc acgtatcgaa tgacgcggtg
     1141 atccggccac ccaagctgct cataaaaact cttttccgtg aggacgctga agaatccccg
     1201 caaggggtgg tcgggaccga tacgcgacga ggggttcatc acctgctccg cgattcgacg
     1261 ggattacacg acatctgagt atagtgggcc gcgtggaatc cgacaagcgg acggagccgc
     1321 tcccgccgcg gcggacctac ttcgcagcgg tcaggaggcc gttgagcgcg gcattgagcg
     1381 agagaggatt gacgcgagct ccgttaaacc gaacgcccca gtgcaggtga ggacccgagg
     1441 cgcgtccggt cgccccgacc ttcccgatca cctggttctt tgccacatga tctccgacct
     1501 tcacgtcggt ctcggaaagg tgaaaataca tcgaatacag tcccgtcccg tggtccacca
     1561 cgatgctctt gccgctgaag aactgctcct ccacgagacg gacgacccca tgattgatgg
     1621 ccaccaccgt cgctccggcc ggcgccgtga tgtcttctcc ggagtggggc cgtctcggtt
     1681 gtccgttgat cacgcgtcgc cggccgaacg tgtgctggag cggcccttcg ttcggcagca
     1741 caaacgcccc atcccagagt cgcggggtca cctcctgcat cgacgccaac atcacgcgtt
     1801 gatcctcttc aacccgtcgc agcgtttgtg gatccagatc cacctgccga tcaggaaggg
     1861 acaaggtttg tatgccgaag tctccctttt caaccgtcac ggtgccgcgc gtcatcactc
     1921 cggggttttc gccgcttttg cgttccacca cgaattcccg aacgccgggg ggggtttcca
     1981 tatcgacgcc gatcagcacg ccgacgcctc ccgacgtgcg gaacagcggc cactcgtgat
     2041 cgccaaaacg cgcgacaaga ccactcgtgt ccgatgcgcc atccatcacc ccggggtcaa
     2101 tggcaagcgc gaacacgtcc ccctgcttga cggtcttggt cggagctggg acatcgccag
     2161 cagcccaggc cacgctcgca aggaacgcgg tcgcgccaac gagcaggggg acgaccttcc
     2221 ccagccgatc agcgttggga ggcggcgaca ccggcgatga gttcttcaac gcgcttgttc
     2281 gagctggcga atgggtccat gcagaggatg gtttcctccc agtacccgtt cagtttgaga
     2341 taggtccaat cgacggtata cgaccggttc ttgtccttgg cgaatttgat gaagtcgccc
     2401 cggaccttgg cacgcgtcgt ctgcggcggc tccgccatcg cgtattgaat ctcggactcc
     2461 gtggtcaccc gatcaaccag cccctgacgt tcgagcaggt agtagaggcc acggtctctc
     2521 ttcacgtcgt gatactgcag gtccatcaag gccacgcggg ggtcatccca cccgcacttc
     2581 ttgcggtcaa taaacgactc catcagattc ttcttgatca cccagtccag ttcggtggcg
     2641 agctgcatcg gatcctccgc caaccgttcc aacaccgatc cccagtacgc cacgagatct
     2701 ttggtggtct catccgccga ggaccccatc gcggccacat aagcctgggc gcgctcccag
     2761 tacgccatct gaatttcgag cgccgtcaac tcccggccat cctccagccg gacccgcttc
     2821 ttgagttggg ggtcgcggga aatatcgcgg atcgctttca cgggatcttc gagctccatc
     2881 cccttgacgg tgtagccttc ctcaatcatg gaaagcacca gcgcgatcgt gccgaccttg
     2941 agataggtcg acaattccga catgttggaa tcgccgacga tgatgtgtaa ccgccgatat
     3001 ttctccgcgt cggcgtgggg ctcgtcacgg gtgttgatga tgctccgcga cgaggtcgtc
     3061 gacgacgacg ttttctcgtg gatgtgttgc gcccgttgtg acaggaaaaa atgcgtccgc
     3121 ccggaaacct tcaagacctt tccggctccg gcgaagatct ggcgcgtcac gaagaacggg
     3181 atgatctgct cggtcacttt ccagaaatcc aggtcccgcc gcatgagata attttcgtgg
     3241 caaccgtagg tattgcccat cgagtcggtg ttgttcttaa aaatatagat ctcgccgacc
     3301 aggccctcct cacgcaaccg tttctcggcc atcgggaggc acgcctccag caggcgctcg
     3361 ccagccttgt cgtgcaccac cagatcgtgc aggcggtcgc actcgggagt cgagtattcg
     3421 gggtgacagc cggtatcctg gtaaaatcgc gcgccgttgc acaagaaagc gttggacggc
     3481 cagctgttgg ggatcagtcc ctcgaagata taccccagga tcttctccat ggggagatac
     3541 actcggccgt tgggcgaaaa gatcaggcca tactcgcttt cgagcccaaa aatccgaggg
     3601 ggcatcatgc ggactatccg gtcaaagcgt tcaactcggc ggctgggaca cgacggaatt
     3661 tccgcccggt gcgtcgacgg tccaggaccg ccacttccag ttgagacaac cgcaggcctt
     3721 ggtgcccgac gctctccagg gcctccaagc ccaatgcaag ggcggctgcc agaggacgcg
     3781 ggccgttcac atgttgtttg aggtacgaga gtatggcttc ggatcgaccg ccgatcacgg
     3841 cgaccaaccg ctcgtcaaag atgctgccgt cgaacgaaat ccgatagatc tcggttgcag
     3901 ccccctcgcc caacccgatg accagaattt ccacttcaag cggtttgatc tcctggctga
     3961 acaccgtccc cagggtctga gaataggcgt tcgccagtga cttggtggtc acgtcctcgc
     4021 gactatagag gtagcccttc atatccgcgt ggcgaatgcc ggcctttctg agattttcaa
     4081 attcgctgta tttcccggcg ccagcaaacg cgatgtgatc gtagatttcc gaaatcttat
     4141 tgagggacac gttgggattg tccgccatca acaggagccc gtgttcgtat tccatcacca
     4201 cggtcgaccg gcctctcgcg atgcccttct tcgcgtactc cgccttgtcc tgcatgatga
     4261 gttcaggcga cacatagtac ggcaaggcca tttacgactc ccgtcgtttc tcgaggaagg
     4321 ccgcgtacac gggcgcgatc cgatcgacgt ccacgtcggt gatcccttga gcggtcacca
     4381 gcttcacgga ggggaagatg cctcgagtcg gatccggaga tcccgtcgcc acgtcttcct
     4441 cggccgcgtt gaacaacgcc tccagcgaga ggttcagcgc gtctcgctcg ttcaatcccg
     4501 ctttgaaata ctgcttgatc gtggtccggg cgtctttccc gccggagccg gtggcatggt
     4561 aatcggcttc ttcgtatcgc ccacccgcca cgtcgtactt gaaaatccgg ccgacgctct
     4621 tgcggtgatc gaagcccgcg aacaagggaa tcaccaccag cccttgaaag gccgcgggga
     4681 ggttgccctt caccatttgg gcgagcttat tggccttgcc gtcgacggag agcgtcgtcc
     4741 cctcgatctt ctcgtagtgt tcaagttcgg tctggaacag acgcgccatt tcaagacagg
     4801 gccccgccgc acccgagatc gccatcgccg accattcatc gaccttgaac accttctcga
     4861 tgcgtcggcc tgaaatctgg tagccctcgg tcgcccgccg gtcacccgcg accacaaccc
     4921 cgtcggcata ccgaagcgcc agcaccgtcg tgccatgcgg gaccgacgcg gcgagcgaaa
     4981 agcccgctcc gcccgacggg gtcgcgtcag ccggtcggtg aggctggagg tacgagtact
     5041 gagtgatcag caggtcataa aaactcgacc cgaggccatt cgtctccccc catccggggt
     5101 cgcgcaccac gggctactcg cccccctttt gcacgtagtt cttgacgaac tcctcggcat
     5161 tctcttcgag gacggcgtcg atctcgtcga ggaggttgtc catctcctcc ttcagctcgg
     5221 cgcccttctc ggcgagcttc tgggcgcctt caccgtcctg gcctccgcct tcgt
//
LOCUS       DBNGTT010000082         2541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3467243, whole genome shotgun sequence.
ACCESSION   DBNGTT010000082 DBNGTT010000000
VERSION     DBNGTT010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3467243"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="AC16BC_01900"
     CDS             complement(<1..357)
                     /locus_tag="AC16BC_01900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HIR3240396.1"
                     /translation="MQIRVYYEDTDCGGVVYYANFLRYFERARTEYLRDRGIEVAAYA
                     TAGLLFVVSHAEVSYRSPGRYNDVLEVETDVVDLTRTSLTFGHEIHRVGDDRLIVEGA
                     ARLVCVDERGKPRRLPE"
     gene            complement(357..1262)
                     /locus_tag="AC16BC_01905"
     CDS             complement(357..1262)
                     /locus_tag="AC16BC_01905"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HIR3240397.1"
                     /translation="MSARSHAPILLVHGGCGSGRPGPRALAVIARALDAGYGLLERGN
                     SPVDAVEAAVVVLESSGRFNAGVGAYRQLDGVARRDASIMDGCSLAAGAVASITGVRN
                     PVRAARRVMDASPHVLLAGSWAERFARHHKIPRSSEPRPATSTSWNALYRRIMKSAKP
                     ASGTVGAVAIDARGHVAAATSTGGIARMLPGRVGDSPLIGAGTYADDQAGAVSMTGTG
                     ETIIRAGLARLIAADMARGIAPKTAGRRALAWMRRRIGGDAGAIILSRTGALALLHTT
                     LHMACGVRDRSGRRVAPHGDRLEER"
     gene            complement(1259..>2541)
                     /locus_tag="AC16BC_01910"
     CDS             complement(1259..>2541)
                     /locus_tag="AC16BC_01910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3240398.1"
                     /translation="PWRTLLTWSADDSLNSQPVGFSKDGTAMYLEDASGSDTNQLVEL
                     RLTDGARRVLAHDERFDIGGVMVHPDTYEIQAVAFDRARTEWIVLDPSIRKDFDAIRG
                     LHHGDVSVISRDDADRTWIVAFTSDREPTAFFAYDRRTKRGRLLFHSKPDLLNYTLAP
                     MEPIDLTSRDGLTLHGYITYPPGLERRGLPLVVNVHGGPHARDAWGIDPEVQWLANRG
                     YACLQINFRGSTGFGKRFLNAGDKEWGGKMHDDLVDAVQWAVGAGIADPKRVAIYGGS
                     YGGYAALAGATFTPDLFCCAVDIVGPSNLITMIETIPPYWEAFRAIEHRRVGDPATEA
                     EFLKSRSPLFFVDRIKAPILIAHGANDPRVKQAESEQIVDAMKRKGIPYEYVLFPDEG
                     HGFAKPENRLRFYAAAERFLAKHLGGRVEAEATA"
BASE COUNT          411 a          870 c          835 g          425 t
ORIGIN      
        1 ctcgggcagg cgacgcggtt tgccgcgttc gtccacgcaa accaggcggg ccgcgccctc
       61 gacgatgagc cgatcgtcgc ccacgcggtg aatctcgtgg ccaaaggtga ggctcgtccg
      121 ggtcaggtcc acgacgtcgg tctccacctc caacacgtcg ttgtatcggc cgggagagcg
      181 gtacgagact tcggcgtgcg agaccacgaa gagcagaccc gcggtcgcgt acgccgcgac
      241 ctcgatcccg cggtcgcgta aatactcggt gcgcgcgcgc tcgaagtagc gcaggaagtt
      301 ggcgtagtac accacgccgc cgcaatcagt atcctcgtaa tacacgcgaa tctgcattac
      361 cgttcctcca atcggtcgcc gtgtggcgcg acgcgtcggc ccgagcggtc tcggaccccg
      421 caggccatgt gcaacgtggt gtgaagaagc gcaagcgctc cggtgcgcga gaggatgatg
      481 gcgcccgcgt ctccgccgat cctccggcgc atccaggcca aggcccgacg acccgcggtc
      541 ttcggcgcga tgccgcgcgc catgtccgcc gcgatcaacc gtgcgagtcc ggctcggatg
      601 atggtttcgc ccgtgcccgt catggacacc gcgcccgctt gatcgtccgc gtaggtaccc
      661 gcgccgatca acgggctgtc ccccacgcgg cccggcaaca tgcgggcgat gcctccggtg
      721 gaggtcgcgg ccgcgacgtg tccgcgcgca tcgatcgcga ccgcgccgac cgtgcccgag
      781 gcgggcttgg ctgacttcat tattcggcga taaagcgcgt tccaggaggt cgacgtcgcc
      841 ggacgcggct ccgacgaacg gggaatcttg tggtggcgcg cgaaccgctc ggcccatgaa
      901 cccgccagga gcacgtgcgg cgatgcgtcc atcacccgcc gcgccgcgcg caccggatta
      961 cggacgcccg tgatcgaggc cacggccccg gccgcgaggg agcagccgtc catgatcgag
     1021 gcgtcgcgtc gcgccacgcc gtccaattgc cgatacgcgc ccacgcccgc gttgaatcgt
     1081 ccgctggatt ccagaaccac caccgcggcc tccacggcat ccaccgggga gttgccgcgt
     1141 tcgagcagtc cgtaccccgc gtccaaggcc cgcgcgatca cagccagcgc gcgtgggccg
     1201 ggtctgccgc tgccgcaacc cccgtgcacg agcaagatcg gcgcgtggct ccgcgcgctc
     1261 acgccgtggc ctccgcctcg acgcggccgc cgaggtgttt ggccaggaat cgctccgcgg
     1321 ccgcgtaaaa ccgcagacgg ttctcgggtt tggcaaagcc gtggccctcg tccgggaaca
     1381 acacgtactc gtacggaatc cccttgcgtt tcatggcgtc cacgatctgt tcggactcgg
     1441 cctgtttcac gcgcgggtcg ttcgcgccgt gggcgatcaa aatcggcgcc ttgatgcgat
     1501 ccacgaagaa caacggggag cggcttttga ggaactcggc ttcggttgcg gggtctccca
     1561 cccggcggtg ttcgatggcg cggaacgcct cccagtacgg cgggatggtt tcgatcatgg
     1621 tgatcaggtt gctgggccct acgatgtcca ccgcgcagca aaagaggtcc ggagtaaatg
     1681 tcgcgcctgc cagcgcggca tacccgccgt acgatccgcc gtaaatggcc acgcgcttcg
     1741 ggtccgcgat cccggctcca accgcccatt gcacggcgtc aaccagatcg tcgtgcatct
     1801 tgccgcccca ttccttgtcc cccgcgttca aaaaccgttt gccgaatccg gtcgagccac
     1861 ggaaattgat ctgtaaacac gcgtagccgc ggttggcgag ccattggact tcggggtcga
     1921 tgccccacgc gtcccgtgcg tgcgggccgc cgtgcacgtt gacgaccagg ggcaacccgc
     1981 ggcgttccag cccgggcggg tacgtgatgt agccgtgcaa tgtgagaccg tcgcgcgacg
     2041 tcaggtcgat cggctccatc ggcgccaagg tgtaattgag caggtccggc ttgctgtgaa
     2101 aaagcagccg gccgcgtttg gtgcggcggt cgtaggcgaa aaacgcggtg ggttcgcggt
     2161 cggacgtgaa ggccacgatc caggttcgat ccgcgtcgtc gcggctgatg acggacacgt
     2221 cgccgtggtg gaggccccgg atcgcgtcga aatctttacg gatcgacgga tcgagcacaa
     2281 tccactcggt gcgcgcgcgg tcaaaggcca cggcctggat ttcgtacgtg tccgggtgga
     2341 ccatgacccc gccgatgtcg aaccgttcat catgggccag cacgcggcgc gcgccgtcgg
     2401 tcaaccgcaa ctcgaccaac tggttcgtat cactcccgct ggcgtcttcc agatacatcg
     2461 ccgtgccgtc tttcgagaag cccacgggtt ggctgttgag gctgtcgtcc gcgctccacg
     2521 tcaggagcgt tcgccaaggg g
//
LOCUS       DBNGTT010000083         3824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1039793, whole genome shotgun sequence.
ACCESSION   DBNGTT010000083 DBNGTT010000000
VERSION     DBNGTT010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1039793"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..586)
                     /locus_tag="AC16BC_01915"
     CDS             complement(365..586)
                     /locus_tag="AC16BC_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3240399.1"
                     /translation="MSGNGARHGADPSIRNAVERLERDLITRSLADANGSQSAAARRL
                     GISERVLRYKMKKYGIVRSTKVSVVDKSV"
     gene            complement(594..3569)
                     /locus_tag="AC16BC_01920"
     CDS             complement(594..3569)
                     /locus_tag="AC16BC_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3240400.1"
                     /translation="MTTRVVVVGNGMAGIAAVEAMVGRPHPPRITVFGAEPYVNYNRI
                     LLSDVLAAAKSVQDIILNPREWYDRHGIDLYTGTSIASLDLANRAVVTQEGARVRYDR
                     LLLATGSTPFLPVINGLDKPGVFTFRNIDDTEAMIQWAAAHRRGVVVGGGLLGLEAAR
                     GLTNRGMQVTVVHLAGHLMEQQLDAGAGALLRRRIEKMGIAVRPSCTVEAVLGEDAVA
                     AVRLSTGEVIETDLVLITAGIRPNVQLARDAGLAVNRGVLVNDAMATSVDGVYAVGEC
                     VEHRGQTYGLVAPLIEQAKVAADAIVGEGRLRYEGSLTHATLKVAGIELTSMGQVTER
                     SDADEVLLACDTGLETYKKLVIRDQRIVGAIFLGDSSGSHVIREKMTAGTDVSAERAT
                     LLAGGEAAPVSVVDLPDGATICNCNGVSKGDIMAAIQEQGCTSRQQIAECTRATTGCG
                     TCAGLVDELVALLAPSPAQGATGAVKPPNKVEQLKKTKPGLDVLPDLSRYAKEGWEAI
                     TEDDVQRLKWYGVFLRTPTPGQFMLRVRVTNGIATALQFREFARIARESGRGFAELTT
                     RQQVQLRWMTIDQVPAILDALAAVGLTTMQTGMDNIRNVMGCSVTGLAPTEVVDTASI
                     TRDLTGRFINNREFTDLPRKFNIVITGCRENCTHSESQDVAMTPASRTIDGETVWGFN
                     VAVGGKQGSGGFTAATPLDLFVRPDDAVDVASAIILAFRDHGSRESRNKSRLAFLVQA
                     WGAKRFRADIEQRVGRPLLRAGTDERNQRKKNTHIGVFRQKQAGLNYVGLAVPIGRLT
                     TEQLDELARLAEEYGTGELRITPEQNVVLVNVPDRKLGALLADEPLLRDVPYNPSEIM
                     RSLVACPGTDYCGLALIETKARALAIARSLEQKLSINKPVSIHWSGCPSGCGNHLMAD
                     IGLLGKRAKIGGRIVDAVDIFVGGRSGPRTEPGTKIMEDVPCDGLEVVLEGLARHVIR
                     DKSVEVVHGDGGERERNGA"
BASE COUNT          663 a         1330 c         1126 g          705 t
ORIGIN      
        1 gcttcgggtg cggccggttc ctccgccggg gccggagctt cgaccgcggc tggcgcctcc
       61 ccgccgccct cctgcgccat cccgaccagc gggacgacca gacccaacag gatgattgca
      121 agacccagca gacttgagac cactcgcttc ctacccatag accgtactcc ttccgtcgaa
      181 cgttcgtgaa agggtgaaca ccactcgccc gtcaccatca ccccgcagtc aggtagagct
      241 atagcaagcg cggggccaac attgggcacc gggatgaggt cgtcctatca cccggaaaat
      301 cctcgtgaaa tccgattcgg gattacgcct ccgacttgtg agcgtcgtgc atgcgacaac
      361 gaggtcagac acttttgtcg acaacggaca cttttgtcga acgcacgatg ccgtacttct
      421 tcatcttgta acgcagcacg cgttcgctga tcccgagtcg gcgcgccgcc gcggactgac
      481 tgccgttggc gtcggccaat gaccgcgtga tcagatcgcg ctccaaccgc tccaccgcgt
      541 tgcgtatcga gggatcggca ccatgtcgcg ctccgttgcc gctcataacg tcatcacgct
      601 ccattccgct cgcgttcgcc cccgtcgcca tgcacgacct cgacggattt atcccggatc
      661 acatggcgcg caaggccctc gagcacgacc tctaatccgt cacacggcac gtcttccatg
      721 atcttggtac cgggctcggt ccgcggccct gatcgtccac cgacaaagat atccaccgca
      781 tccacgatgc gaccgccgat cttggcgcgt ttgcccagca agccgatatc cgccatcagg
      841 tggttgccgc acccggaggg acagccggac cagtggatgg agacgggttt attgatcgag
      901 agtttttgtt ccaacgaccg cgcgatcgcc agcgcccgcg ccttggtttc gatcaacgcg
      961 agaccgcaat agtccgtccc cggacaggcc accaaactcc gcatgatttc ggacggattg
     1021 tagggcacgt ccctgagcag gggttcgtcc gccagcaagg cgcccaattt gcggtccgga
     1081 acgttgacca gcaccacgtt ctgttccggc gtgatgcgga gttcgccggt gccatactcc
     1141 tcagccagtc gggccagttc atcgagttgt tcggtggtca gacgcccgat cggcaccgcc
     1201 agccccacgt agttgaggcc tgcttgcttc tgccggaaca cgccgatgtg cgtgttcttt
     1261 ttgcgctggt tgcgttcgtc ggtcccggcc cgcaagaggg gccggccgac ccgttgttcg
     1321 atgtcggcgc gaaaccgctt ggctccccac gcttgaacga gaaacgcgag gcgcgacttg
     1381 ttgcgagact cgcgcgaacc gtggtcgcgg aacgccaaaa taatggcgct cgccacatcc
     1441 accgcgtcgt cgggacgcac aaacagatcc agtggggtcg cggcggtgaa cccgccggag
     1501 ccctgcttcc cgccaaccgc cacattgaat ccccacacgg tttcgccgtc gatggtccgg
     1561 gaggccggcg tcatcgcgac gtcctgggac tcggagtgcg tgcagttctc ccggcagccc
     1621 gtgatcacga tgttgaactt gcgcggcaga tccgtgaact cgcggttgtt gatgaaccgc
     1681 ccggtgagat cccgcgtgat cgacgcggtg tccacgacct cggtgggcgc aaggcccgtg
     1741 accgaacacc ccatcacgtt ccggatgttg tccatgccgg tctgcatcgt ggtcagcccc
     1801 acggccgcga gcgcgtccag gatcgcgggg acttgatcga tcgtcatcca ccgcaactgg
     1861 acttgctgcc gggtggtcag ttccgcgaag ccacggccgg actctcgggc aatcctggcg
     1921 aattcgcgga actgcagggc ggtggcgatg ccgttggtca cccgcacgcg cagcatgaac
     1981 tggccggggg tgggcgttcg caggaagacc ccgtaccact tgagacgctg cacgtcgtcc
     2041 tccgtgatcg cctcccaccc ttccttggcg tagcgggaca ggtcgggcag cacgtccagg
     2101 ccgggtttgg ttttcttgag ttgttcaacc ttgttcgggg gtttgaccgc cccggtcgcg
     2161 ccctgcgcgg gggacggcgc caagagggcg accagttcat cgaccaaccc ggcacaggtc
     2221 ccgcatccgg tggtggcgcg cgtgcactcg gcgatctgct ggcgcgacgt acacccctgc
     2281 tcctgaatcg cggccatgat gtcgcctttg ctcacgccgt tgcagttgca gatcgtggcg
     2341 ccgtccggca ggtcgaccac gctcacgggc gcggcctccc cgccggccaa caacgtcgcg
     2401 cgttccgcgg agacgtcggt ccccgccgtc atcttctccc ggatcacgtg actgcccgac
     2461 gaatcgccca agaaaatcgc gcccacgatg cgttggtcgc ggatgaccaa ttttttgtag
     2521 gtttccagtc cggtatcgca agcgagcagg acctcgtccg cgtcggatcg ctcggtcacc
     2581 tgccccatcg acgtgagttc aatcccggcc actttgagcg tggcgtgagt cagcgatccc
     2641 tcgtagcgca ggcgtccctc acccacgatc gcatccgccg ccactttggc ctgctcgatc
     2701 agcggcgcca ccaaaccgta ggtttgaccg cggtgttcca cgcactcgcc caccgcgtag
     2761 acgccgtcaa ccgacgtggc catggcgtca ttgaccagca ccccgcggtt cacggccagc
     2821 cccgcgtcgc gggcgagttg cacgttgggc cgaatgccgg cggtgatcag caccagatcg
     2881 gtctcgatca cttcgccggt tgacaaccgc acggctgcga ccgcgtcctc gcccaagacc
     2941 gcctccacgg tgcacgacgg ccgcaccgcg atccccatct tctcgatccg tcgacgcaac
     3001 aacgccccgg ctccggcgtc gagttgttgc tccatgaggt gcccggcaag atgcaccacg
     3061 gtcacctgca tcccgcggtt ggtcagacca cgcgccgctt caaggcccag cagccctccg
     3121 cccaccacca ccccgcgccg gtgggccgcg gcccactgaa tcatcgcttc ggtgtcatcg
     3181 atattccgaa acgtgaacac ccccggctta tccaacccgt tgatcacggg aagaaacggc
     3241 gtgctgccgg tcgcgagcag cagccggtcg tacctcaccc gcgcgccctc ctgcgtgacc
     3301 acggctcgat tcgcgagatc cagcgaggca atgctcgtcc cggtatataa atcgatcccg
     3361 tgtcggtcgt accactcacg gggattgagg atgatatctt gcacggactt ggcggccgcg
     3421 agcacgtccg agagcaggat gcggttgtag ttgacgtacg gctccgcgcc gaacaccgtg
     3481 atccgcggcg gatgggggcg acccaccatc gcctccaccg cggcgatgcc ggccatgccg
     3541 ttaccaacca ccacaacgcg tgtggtcatc tcatgttctc cattggtatc atcaccagag
     3601 caaaccgcgt accaccgtca catccgatca tcagcgctca aaccgagcgt tgagtctctt
     3661 cctaaccggg tcccgggcgc gttcgacgat aatgtcgaac acttctgtga aacctgaatc
     3721 gacaattctg tcggaagggc gcgccctgag ccgctcagtg cgatgaggcg gcgcgcatga
     3781 cctcgtcggg agtattcaaa ctctgcgcag cgcgtcgcaa cgtc
//
LOCUS       DBNGTT010000084        14140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2317567, whole genome shotgun sequence.
ACCESSION   DBNGTT010000084 DBNGTT010000000
VERSION     DBNGTT010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 14140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14140
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2317567"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..2380
                     /locus_tag="AC16BC_01925"
     CDS             251..2380
                     /locus_tag="AC16BC_01925"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240401.1"
                     /translation="MLDTPVWRGVLLSAVLLLTAFTYRGVTANGYVLDDAHLVASNPS
                     IVSFASAGRWFTTPYATSTLREHPGYRPITVASYAIDRALWPNPAVGFHRTNLLIHLI
                     AVVLVALLARLLWADGTAALAVAAVMALHPINAEAVNYLSARSSSLAALFVLTSIWAY
                     DRAMRPSHGRGASAVWFGAALAAGALALGAKEIAVALPVLILIWDRARFGDHTSWRTS
                     ATRSLPFWAITAAFLAARSIVMGQAPVPSAGASGQTALFAMKIVLTSFGQWFVPVGLA
                     VDRGWPWTIGAAEAAWLSLGFASVAAVTAWAFRYDRRIGWCLIWSWGALAPLAALPAV
                     SRVTLYQDHRVYLAGIGLAWALGGLIAAATRAWTGRAFRWIAAATAAVLVTAMIRADL
                     ARSAVWVDETRLWEDVLSKYPNSVVALNARGLQSLNAGRFDDAERDFQTTLRLVPGSS
                     EAHKNLGLVYAEAGDLDRAVREMNAALALSPGSPGILVELGFVYERAKDWASAKQAYE
                     QVLQSSPSEVLALKGLARAAEREQRFDEAAPRYRAALAVDPRDDHAWLSLGAVLVRLE
                     RWSEARDAFTAVLSRHPGNPQARFNLGAALDGLGDAAGATQAYAQAAASLPRDADLAF
                     RIGLMHAGHGRWAEAAAAYEDALRRDPRHAAGHLGLARVAEQLGDPQRALTHYRAALA
                     SEPPNASVALREQATAAIKRLEGGKGR"
     gene            2461..3843
                     /gene="argH"
                     /locus_tag="AC16BC_01930"
     CDS             2461..3843
                     /gene="argH"
                     /locus_tag="AC16BC_01930"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3240402.1"
                     /translation="MDKPWGGRFSEPTKSTVETFTASIPFDWRLYRHDIAGSVAHCKA
                     LAEAKLIAPAERDQIVSGLKAIEADIAAGKFAFSIAHEDVHMNVERALTERIGPVAGK
                     LHTGRSRNDQVALDMRLYVREEIDALIDLVRGVQKALAEAASRHLDVILPGYTHLQRA
                     QPVLLAHHLLAYYEMLLRDAERLRDARNRVNRMPLGSGALAGTNYPINRELVAEYLDF
                     PIATRNSLDAVSDRDFVIESLAACALIMAHLSRFAEELVIWSTSEFGFIELPDGFCTG
                     SSMMPQKKNPDVPELIRGKTGRVYGSLMALLTTVKALPLAYNKDMQEDKEPLFDAVDT
                     VKACLAITAEMIPGIRFKTDAMTAAASDSFLLATELADALALKGLPFREAHEVVGKLV
                     RYCIETKQPLTQLSLDEFRRFSPLIDAAMVAGLTPQAAIARKSQIGGTAREAVQQRLK
                     ELEKGGRRGG"
     gene            4231..6180
                     /locus_tag="AC16BC_01935"
     CDS             4231..6180
                     /locus_tag="AC16BC_01935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3240403.1"
                     /translation="MASSTLVEPTEKRSSVSVQSRKRRLLLIEPYPEDSPYRFTQGER
                     RALWFPKLSLPVIAAYTPDHWDVTFVDEAVDRVDFSLDVDLVGISAQMTCYAPRSYQL
                     AERFRARGIKTVIGGTHVSYCPDEALQHADTILIGESEAIWPQVIKDFEAGLMQRTYR
                     MAEFPSMDDYRLPRVDLLKHEEYMTTSCIQTTRGCHFDCEFCSVSPYNGKTSRRRPVS
                     HVVAEIQRIKDRRRSLIVDKMTSGPYGRRFLASVKLLSGIEDGTIFAFVDDLHNSSRA
                     YCKDLWTALIPLKIKWGAQCTLFLGDEPEMVKLAAESGCVSMFVGLESIIEESIDETN
                     KPFNRVAQYEREIKCFHDHGIMLNPGIIFGFDNDDESVFARTVEFLNRNRMELAYFNI
                     LTPLPGTALFERMKGEGRIFDFNWANYDGKHVVFWPKRMSPKTLQDGFFWANHEFFSW
                     PSIARRILPTGQRLAARTAMNLAFRHLVRRTAPKGSISPLSEVIQSLRLKLPTLATEN
                     LIPNALHALKEKATGAAQQIDRFLQVQVKRSDVARAADEAAPPRPALHLELVGTLDEL
                     GAKELRKRLLDAVREAKLEIVVNFEHVRHATPQAVQSLIDGEYLKALKAHASVKFINL
                     KTAFQAAVERISPPRPGVWGDETIG"
     gene            6336..7601
                     /gene="lysA"
                     /locus_tag="AC16BC_01940"
     CDS             6336..7601
                     /gene="lysA"
                     /locus_tag="AC16BC_01940"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3240404.1"
                     /translation="MHDFPDIDGQLYCEQVPVAKIAKAVGTPCYIYSHQTLTRHFQAV
                     DGAFASIPHVTAFAMKANSNLAILRLFASLGGGADIVSGGELYRALKAGVPRNKIVFA
                     GVGKTREEIAEALKAKILSFNVESAQELATIDEVARKLKTRARISLRVNPHIDPKTHP
                     YISTGLKKSKFGIGIDQALDHYKQASTLKGIEIVGVHHHIGSQLTQVQPFVEALARVL
                     ELVDALRTQGVVIRSLDMGGGLGIPYKDETPPSPRDLANAVLPLLRGRDLTLIMEPGR
                     VLVGNAGILVTKVLYTKEGAEKNFVVVDAGMNDLLRPSLYEAYHEIRPVRRAQRSPRV
                     VDVVGPICESGDFLAQDRELPSVEPGELLAVMSAGAYGFSMASRYNSRPLIPEVMVKG
                     NRFVVIRERETYRDLVRGETIPAFLKGGR"
     gene            7601..8017
                     /locus_tag="AC16BC_01945"
     CDS             7601..8017
                     /locus_tag="AC16BC_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240405.1"
                     /translation="MATAAETTAQAKPGDRKGRGFFLTLIIASGLVILGLAFVYFKGK
                     DFFIFTWQKFVIEKALVRALPPDYSVEQVEDIRRALIDFYAAGGRHEIPDEALYGMSQ
                     GIKTILADQVVTKQEAEALMAFIREHGPKTEGAAAP"
     gene            8021..8854
                     /gene="dapF"
                     /locus_tag="AC16BC_01950"
     CDS             8021..8854
                     /gene="dapF"
                     /locus_tag="AC16BC_01950"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR3240406.1"
                     /translation="MARTSKIPFAKMSGSGNDFILIDNRKEIVPRRKMRAFVKSVCRR
                     GLSVGADGVIFIQPSRRADFRWHYFNSDGGEAEMCGNGSRCAARFATLKKIAPKQMTF
                     ETLAGIIHADVGAKGVTVTLPLPHDLRLNVTVEVGGKSYDAHFLNTGVPHTVLFLDDV
                     EDVDVQGLGRAIRFHPLFAPTGTNANFVRIDGPRHLTIRTYERGVEAETLACGTGSVA
                     AAVLAGLLGKVSPPVTLTTRGGPPLIVGFELEGHAIRRVELTGEARLVYEGTLSPEAL
                     L"
     gene            8854..9726
                     /gene="dapA"
                     /locus_tag="AC16BC_01955"
     CDS             8854..9726
                     /gene="dapA"
                     /locus_tag="AC16BC_01955"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3240407.1"
                     /translation="MFTGSYVALVTPFKNGKIDEKALGDLIEWQIDRGTNGLVPCGST
                     GESATLTHEEHDRVIELTVEVARRRVPVIAGTGSNATHEAIRLTKHAKSVGVDAALLV
                     TPYYNKPTQEGLFRHFEAIARAVDLPLVLYNIPGRSVVNMTPQTVARLSAITNIVGIK
                     EASGNLSQMSDVIAACGERFVVLSGDDALTLPLMAVGGKGVISVTANVAPAETADMVS
                     AALKGDWDRAKRVHYRLLPLSNSMFLETNPIPVKAALAMMGRCADELRLPLTTMSDAP
                     KAQLRQALLQFGLV"
     gene            9908..10717
                     /gene="dapB"
                     /locus_tag="AC16BC_01960"
     CDS             9908..10717
                     /gene="dapB"
                     /locus_tag="AC16BC_01960"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3240408.1"
                     /translation="MKPTRLIIMGAGGKMGQRLVALATETPGLALGAAVERAGHQAVG
                     RDAGEAAGVGRLGIAISDDVKRALSDGEVVIDFTSPAATAALIPLALATKRPLVIGTT
                     GMSPEGLRAIRDAAASLPIVFAPNMSLGMNVMFKIVADVARALGPGYDLEIIEAKHRL
                     KKDAPSGTALRLAEVLADATKRRLDEVGVYARHGEIGARTPNEIGIQTIRAGDIVGDH
                     TVLFGGLGERLEITHRASSRDTFARGALRAAQWLAGKAPGLYDMQDVLGLR"
     gene            11092..11835
                     /locus_tag="AC16BC_01965"
     CDS             11092..11835
                     /locus_tag="AC16BC_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3240409.1"
                     /translation="MDLLTDPNAWLALITLTALEIVLGIDNIIFISILAGKLPEAQRA
                     RARTIGLAFAMGTRLALLASLVWMMRLTAPWFIVLDQEISGRDLILIAGGLFLLAKST
                     MEIHDKLEGEEESSSGRSATTFASVIVQIAILDIVFSLDSIITAIGMANQLPVMVAAV
                     IIAVIFMMFFSGAVSAFIDRHPTVKVLALSFLLMIGLALIGDGLDMHIPKGYIYFAMA
                     FSVFVEMLNIRVRKKVEPVHLRGPHAPSK"
     gene            complement(11836..12906)
                     /locus_tag="AC16BC_01970"
     CDS             complement(11836..12906)
                     /locus_tag="AC16BC_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR3240410.1"
                     /translation="MDPQHPTPRGVHGRGAAGNPRNRFEGVERVPEPIDAADAEQPLP
                     VTQFLSDASKTIIARNNSPDVGFDAGVNPYRGCEHGCAYCYARPTHEYLGFSAGLDFE
                     TKIMVKHDAPELLRRELSKRSWTPRLIAMSGVTDCYQPIERKLRLTRQCLEVLADFRN
                     PVVIITKNALVTRDADVLSRLAEHHAVAVVLSITTLDADLCGALEPRTARPAKRLAAI
                     EALAQAGVPVGVNVAPVIPGLNDAEIPKITQAAADAGATFAGLVPLRLPYGVAPLFEA
                     WLDQHRPGMKEKVLHRMRDLRGGSLNDPNFGSRMTGQGPFADQIHALFAMACKRAGLT
                     GWPELSTAAFRRPTAQASLFDG"
     gene            complement(12907..13263)
                     /locus_tag="AC16BC_01975"
     CDS             complement(12907..13263)
                     /locus_tag="AC16BC_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR3240411.1"
                     /translation="MLTFYGYAKCSTCVNAKKFLAKAGHRFRDIDVTTSPPSADELRG
                     LIARSGRPYTDFLNRSGLQYRALNMKDKVKTLPEDAIIKQLASEGRLIKRPIVTDGTH
                     VTVGYSPEEFQKIWGR"
     gene            13410..13850
                     /locus_tag="AC16BC_01980"
     CDS             13410..13850
                     /locus_tag="AC16BC_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3240412.1"
                     /translation="MFPPFVLWLSLGVVLLIVEVVTGGFWIGFFGVGGLVTALALWTD
                     AIAGLNAQLAVFLIASVLPLVVLRKPLMAWLNRDVQTSRFNDAVGQIAVVVQDIVPPG
                     VGRVEYQGSTWEADSAAGERLAANTRVQIVRQDGTRLSVRAISE"
BASE COUNT         2420 a         4390 c         4764 g         2566 t
ORIGIN      
        1 cgtcgatctc gccacgtttg aggaagaggc cgtgtacgat caaaaagacg cggcggggtt
       61 catccggctc aacgcattgc ggttgaaggt cggcagccga acccgacgga agtagccccg
      121 gactcgctgg cgcgagtccg aacgtggtca ccgtggtcac cgtgagtaca cgaggggctc
      181 agtacgaatg tccgcgccgg cgtcgacagt ctaattcgct ccccggccgc ccgtgctttc
      241 cttccgcatg atgctcgaca ctccggtgtg gcgaggggtg ttgctgagcg cggtcctgtt
      301 gctcacggcg ttcacgtatc gcggggtgac cgcgaacggg tacgtgctcg acgacgcgca
      361 cctggtggcg agcaacccct cgatcgtttc gttcgcatcg gccggccggt ggttcaccac
      421 gccctacgcg accagcacgc tgcgggagca tccggggtac cgcccgatca cggtggcgag
      481 ttacgccatc gaccgcgccc tgtggcccaa cccggcggtc gggtttcacc gaaccaatct
      541 tctgattcac ctgatcgcgg tggtgttggt tgcgctgctg gcccgtctgt tgtgggccga
      601 tggcacggct gcgttggcgg ttgccgcggt gatggcgctg caccccatca acgccgaggc
      661 cgtcaactac ctctcggcgc ggtcgtcgtc gcttgccgca ctgttcgtgc tgacgtcgat
      721 ctgggcgtac gaccgggcga tgcgtccatc ccatgggcgg ggcgcctcgg cggtctggtt
      781 cggcgcggcg cttgcggccg gtgcgctggc actgggcgcg aaagagatcg cggtagccct
      841 gccggttctg atcctgatat gggatcgcgc gcggttcggg gatcacacct cgtggcggac
      901 gagcgcgacg cggtcgctgc cgttctgggc gatcaccgcg gcgtttctgg cggcgcgttc
      961 gatcgtgatg ggacaggcgc cggtcccgtc cgccggtgcg tcgggtcaga ccgcgttgtt
     1021 tgcgatgaag atcgtgctga cctcgttcgg tcagtggttc gtgccggtcg gtttggcggt
     1081 cgatcgcggg tggccgtgga ccatcggcgc ggccgaagcc gcctggctgt cgcttggttt
     1141 cgcgtccgtg gcggccgtca cggcgtgggc gttccgttac gatcgccgga tcgggtggtg
     1201 tctgatttgg tcctggggcg cgctcgcgcc gttggccgcg ttgcccgcgg tgagccgggt
     1261 cacgttgtac caggatcacc gcgtgtactt ggccggcatc ggactggcgt gggcgctcgg
     1321 cgggctcatc gccgcggcga ctcgggcgtg gacggggagg gcattccgct ggatcgccgc
     1381 cgcgaccgcc gcggttctcg ttactgccat gatccgcgcc gacctcgccc gcagcgcggt
     1441 gtgggtggat gagacgcggc tgtgggagga cgtgttgagc aagtacccga acagcgtggt
     1501 ggcgctcaat gcacggggcc tccaatccct caatgccggt cggttcgacg acgcggagcg
     1561 ggactttcaa accacgctcc ggctggtgcc gggatcgtcg gaagcgcaca agaacctggg
     1621 gctggtgtac gcggaagcgg gggacctgga tcgagcggtc cgtgagatga acgccgccct
     1681 cgcgctcagc cccgggtcgc cggggattct ggtcgaactg ggttttgtct acgagcgcgc
     1741 caaggactgg gcgtcggcga agcaggccta tgaacaggtt ctgcaatcca gccccagcga
     1801 ggtgctcgcg ctcaaaggct tggccagagc ggcggaacgg gaacaacgat tcgacgaagc
     1861 cgcgccgcgc taccgcgccg cgctggccgt ggacccgcgg gatgaccacg cgtggctgtc
     1921 gttgggcgcg gtgctggtgc gcctggaacg atggagcgag gcgcgcgacg cgttcaccgc
     1981 ggtgctctcg cgccatcccg gcaacccgca ggcccgcttc aatctgggcg cggcgctcga
     2041 cggtttgggc gacgccgcgg gcgccacgca ggcctacgcc caggccgccg cgagcttgcc
     2101 gcgcgacgcg gacctggcgt ttcgcatcgg cctcatgcac gcgggccacg ggcgctgggc
     2161 cgaggccgcg gcggcctacg aagacgcgct gcgccgcgat ccgcgtcacg cggccgggca
     2221 cctgggcctg gccagggtcg cggaacaact cggcgacccg cagcgcgcgc tgacccatta
     2281 ccgcgcggcg ctggcgagcg agccgcccaa cgcgagcgtc gcattgcgcg aacaggccac
     2341 ggccgcgatc aagcgtcttg agggcggcaa ggggcgatga tccgtggacg cccgcctcgt
     2401 tgacagtgag aggcgttcga ccctaggatg aacggcactc acgggagcca aggccggaat
     2461 atggacaagc cctggggcgg acggttttcc gagcccacga aatcaacggt tgaaaccttc
     2521 accgcgtcga ttcccttcga ctggcgcctg tatcgtcacg acatcgcggg cagcgttgcg
     2581 cactgcaagg ccttggccga ggccaagttg atcgcgccgg cggagcggga tcagatcgtc
     2641 agcgggctca aggcgatcga ggcggatatt gcggcgggga aattcgcgtt ttcgatcgcg
     2701 cacgaagacg tccacatgaa cgtggagcgg gcgttgaccg agcgcatcgg cccggtcgcg
     2761 ggcaagctgc acaccgggcg ctcgcgcaac gaccaggtcg ccttggatat gcggctgtac
     2821 gtgcgcgagg agatcgacgc gctcatcgac ctggtgcgcg gggtgcagaa ggcgttggcc
     2881 gaggccgcgt cgcggcatct ggacgtcatc ctgcccggct acacccattt gcagcgcgcc
     2941 cagcccgtgc tattggcgca ccatctgctc gcgtattacg agatgctgct gcgcgacgcg
     3001 gagcggctgc gggacgcgcg caaccgggtg aaccggatgc cgttgggttc gggcgcgttg
     3061 gcggggacca attatccgat caatcgcgag ttggtggcgg agtatttgga cttcccgatc
     3121 gccaccagga acagcctgga cgcggtgagc gatcgggact tcgtgatcga aagcctcgcc
     3181 gcgtgcgcgc tgatcatggc gcatctgtcg cgattcgccg aggaactggt catctggtcc
     3241 acgagcgaat tcgggttcat cgaactgccg gacgggtttt gcacgggcag cagcatgatg
     3301 ccgcaaaaga agaacccgga cgtgccggaa ttgatccggg gcaagaccgg ccgggtctac
     3361 ggctcgctga tggccttgct caccacggtc aaagccttgc cgctcgcgta caacaaggac
     3421 atgcaggaag acaaggagcc gctgttcgac gcggtggata cggtcaaggc gtgtttggcg
     3481 atcacggcgg agatgatccc gggcatccgg ttcaagaccg acgccatgac cgcggccgcg
     3541 tccgacagtt tcctgctcgc caccgaattg gccgacgcgt tggcgctcaa gggtttgccg
     3601 tttcgcgagg cgcacgaggt cgtgggcaag ttggtgcggt attgcatcga gaccaaacag
     3661 ccgctgaccc agttgtcgct cgacgagttc cggcggttct cgccgttgat cgacgcggcc
     3721 atggtggccg gcctcacgcc ccaagccgcg atcgcgcgca aatcccagat cggcggcacc
     3781 gcgcgcgaag ccgtgcagca gcggctcaag gaacttgaaa aaggcgggag gcgtggggga
     3841 tgaaagagat gtatgcccga tccctcgatg tcgtggaacg aactgaccct ggggccgcaa
     3901 ggccctggcc cgacgagaag gcggcgaatg caaggcggag cgaggctttg ctccgcagcg
     3961 tactgggtcg tacgtgagga agcaaagccg aagcgacaac gcagcaggcg ccccttgtcg
     4021 tcgggccatg ctgcttgcat tgttgtgcgg gattttgcta gcattcaccg tcagttgcgg
     4081 ccgaaagggc gctccaattg cgccggaaga cgtgcctaag ccggcgaagc acttctcgca
     4141 gtctggttag acggttggcc cgcagcgacc cgcgtcgtcg tgagcggcga tgcgtccacg
     4201 cgccgtccag ggacggacga aggaggcgtt atggcgtcat cgacgctcgt tgaaccgact
     4261 gaaaagcgct catcggtgag cgtgcaatcg cgcaagcggc gactgcttct gatcgagccc
     4321 tatccggaag acagcccgta tcggttcacc cagggcgaac gtcgagccct gtggttcccc
     4381 aaactctcat tgcccgtgat cgcggcctac acgcccgatc actgggatgt cacgttcgtc
     4441 gacgaagccg tggatcgggt ggatttctcg ctcgacgtgg atctggtcgg catctccgcg
     4501 cagatgacct gctacgcgcc gcgctcgtat cagctcgccg agcggtttcg cgcgcgcggc
     4561 atcaagaccg tgatcggcgg cacgcacgtc agctactgcc cggacgaggc gcttcaacac
     4621 gcggacacga ttttgatcgg cgagtccgag gccatctggc cgcaggtcat caaggacttt
     4681 gaagccgggc tgatgcagcg cacctatcgc atggcggaat tcccgagcat ggacgactac
     4741 cgcctgccgc gcgtggatct gttgaagcac gaggagtaca tgaccacctc gtgcatccaa
     4801 accacgcggg ggtgccattt cgattgcgag ttctgcagcg tgtcgccgta caacggcaag
     4861 acctcgcggc ggcgcccggt ctcgcacgtg gtggcggaga tccagcggat caaggaccgg
     4921 cgccgcagcc tgatcgtgga caagatgacg tcgggaccgt acggccgacg tttcctggcg
     4981 tcggtcaaac tgctgtccgg gatcgaggac ggcacgattt tcgcgttcgt ggacgacctg
     5041 cacaactcca gccgggctta ctgcaaggac ttgtggaccg cgttgatccc gctcaagatc
     5101 aagtggggcg cgcagtgcac gctgttcctc ggggacgagc cggagatggt caaactcgcg
     5161 gcggaaagcg ggtgcgtgtc catgttcgtg ggcttggagt cgatcatcga agaaagcatc
     5221 gacgagacca acaagccgtt caaccgggtc gcgcaatacg agcgggagat caagtgcttc
     5281 cacgaccacg ggatcatgct caacccgggc atcatcttcg gctttgacaa cgacgacgag
     5341 tcggtattcg cgcggaccgt ggaattcttg aaccgcaacc gcatggaact ggcctacttc
     5401 aacattctca ccccgttgcc gggcacggcg ttgttcgagc gcatgaaggg cgagggacgg
     5461 atcttcgact ttaactgggc gaactacgac ggcaagcacg tggtgttctg gcccaagcgc
     5521 atgagcccaa agacgttgca agacggcttt ttctgggcca accacgaatt cttctcgtgg
     5581 ccgtcgatcg cgcggcggat cctgcccacg ggacaacgcc tggcggcccg taccgccatg
     5641 aatctggcgt tccggcatct ggtgcgccgc accgcgccca agggcagtat ctcgccgttg
     5701 tccgaggtga ttcagagcct gcggctgaag ctgccgactt tggccacgga aaatttgatt
     5761 cccaacgcgt tgcacgcact caaggagaag gcgaccggcg cggcgcagca gatcgatcgg
     5821 ttcctgcagg tacaggtcaa acgcagcgac gtggcgcggg cggcggacga ggccgcgccg
     5881 ccgcggcccg cgttgcacct cgaattggtg ggcacgctgg acgaattggg cgccaaggaa
     5941 ctgcgcaaac gcctgctcga cgcggtgcgc gaggccaagc tggaaatcgt ggtgaacttc
     6001 gagcacgtgc ggcacgccac gccgcaggcc gtgcagtcgc tgatcgacgg ggagtatctc
     6061 aaggcactga aggcgcacgc ctccgtgaaa tttatcaacc tcaagaccgc gtttcaggcc
     6121 gcggtggagc ggatctctcc gccgcgaccc ggcgtgtggg gcgacgaaac cataggatag
     6181 gcgcccgtcc atgagcggtc atctgctgcg ttgtcgcttc gaaatccctc ctcacgtacg
     6241 acccagtacg ctccgggggg atttctcgct ccgccttgca tctgacgcgc tcctgaacgg
     6301 gcttcgatgc gttatgacgc aatggggatt tccatatgca tgattttcct gatatcgacg
     6361 ggcagttgta ctgtgagcag gttccggtgg ccaagatcgc caaggcggtc ggcacgccgt
     6421 gttacatcta cagccaccaa acgttgacca ggcactttca ggccgtggac ggggcgtttg
     6481 cgtcgattcc gcacgtgacc gcgttcgcga tgaaggccaa ttccaacctg gcgatccttc
     6541 ggctcttcgc gtcgctgggc ggcggggccg acatcgtgtc gggcggggag ctgtatcgcg
     6601 cgctcaaggc gggcgtgccg cgcaacaaga tcgtgttcgc gggcgtgggc aagacccgcg
     6661 aagaaatcgc ggaagcgctc aaggccaaga ttctgtcgtt caacgtagaa tccgcgcagg
     6721 aactcgcaac gatcgacgaa gtggcgcgca agctcaaaac gcgcgcgcgg atttcgctgc
     6781 gcgtgaaccc gcacatcgac cccaagaccc atccctacat ttccaccggg ctcaagaagt
     6841 ccaagttcgg gatcgggatc gaccaggcgc tcgatcacta caagcaggcg agcacgctta
     6901 aaggcatcga gatcgtgggc gtgcaccacc acatcggctc gcagctcacc caagtccagc
     6961 catttgtgga ggcgctcgcg cgcgtgcttg aattggtcga cgcgctcagg acgcaaggcg
     7021 tggtcatccg cagcttggac atgggcggcg ggttgggcat tccctacaag gacgaaaccc
     7081 cgccctcgcc ccgggacttg gcgaacgcgg tgctgccgtt gctgcgcggg cgcgatctca
     7141 cgttgatcat ggagccgggg cgcgtgctgg tgggcaacgc cggcatcctg gtaaccaaag
     7201 tgctgtacac caaggaaggc gcggaaaaga acttcgtggt ggtggacgcg ggcatgaacg
     7261 atctgctccg gcccagcctg tatgaggcgt atcacgagat ccggcccgtg cgtcgcgccc
     7321 aacgctcgcc gcgcgtggtg gacgtggtcg gcccgatctg cgagtccggc gatttcctcg
     7381 cgcaggaccg cgaactgccg tcggtcgagc cgggcgagct gctggcggtg atgagcgcgg
     7441 gcgcgtacgg gttttccatg gcgtcgcggt acaattcgcg gccgctgatt cccgaggtga
     7501 tggtcaaagg caaccgcttc gtggtgatcc gcgaacgcga aacctatcgc gacctggtgc
     7561 ggggcgaaac gattcccgcg tttttgaaag gcgggcgtta atggccaccg cggccgaaac
     7621 cacggcacag gccaagcccg gcgaccgcaa ggggcgcggg ttttttctga ccctgatcat
     7681 cgcgtcgggt ctcgtgatcc tggggctcgc gttcgtgtac tttaagggga aggacttctt
     7741 cattttcacc tggcagaagt tcgtaatcga aaaagccctg gtgcgcgcat tgccgcccga
     7801 ttacagcgtc gaacaggtgg aggacatccg ccgcgcgctc atcgactttt acgcagcggg
     7861 cggccgccac gagatcccgg acgaggcgct ctacggcatg agccagggga tcaaaaccat
     7921 tttggccgac caggtggtca cgaagcagga agccgaggcg ctcatggcgt tcatcagaga
     7981 acatgggccc aagacggagg gcgccgcggc gccttaattc atggcacgga cgagcaagat
     8041 cccgttcgcc aagatgtcgg gcagcggcaa cgacttcatc ttgatcgaca accgtaagga
     8101 gatcgtgccg cgccgcaaga tgcgggcgtt cgtgaaatcc gtgtgccgac gggggttgtc
     8161 cgtgggcgcg gacggggtga tcttcatcca gccgtcgcgg cgcgcggatt ttcgctggca
     8221 ctatttcaat tcggacgggg gcgaggccga aatgtgcggg aacggcagcc gctgcgccgc
     8281 gcggttcgcc acgctcaaga agatcgcgcc aaaacaaatg acctttgaaa cgctggcggg
     8341 catcattcac gcggacgtcg gcgccaaggg cgtgaccgtc acgttgccgc tgccgcacga
     8401 tctgcggttg aacgtcaccg ttgaggtcgg cggcaagtcc tacgacgccc acttcctcaa
     8461 caccggcgtg ccgcacaccg tgctgtttct cgatgatgtg gaggacgtcg acgtgcaggg
     8521 gctggggcgc gcgatccggt tccatcccct gttcgcgccc acgggcacga acgcgaattt
     8581 cgtgcggatc gacgggccgc gccacttgac catccggacc tacgagcgcg gggtggaagc
     8641 ggagaccctg gcctgcggca cgggctcggt ggccgccgcc gtgctcgcgg ggttgctcgg
     8701 caaggtgtcg ccgcccgtca ccctgaccac gcgcggcggt ccgccgctga tcgtcgggtt
     8761 cgagttggag ggacacgcga ttcggcgcgt ggagttgacg ggcgaggctc ggttggtgta
     8821 cgaagggacg ctcagtccgg aggcgttgct ctgatgttta ctggttcgta cgttgcgctc
     8881 gttacgccgt tcaagaacgg caagatcgac gaaaaggcgt tgggcgatct gatcgaatgg
     8941 cagatcgatc ggggcaccaa cggcttggtg ccgtgcggca gcacgggcga gtccgccacg
     9001 ctcacccacg aagagcacga tcgcgtgatc gagttgaccg tcgaagttgc gcgtcggcgc
     9061 gtgcccgtga tcgcgggcac cggctcgaat gccacgcacg aggccattcg cctgaccaaa
     9121 cacgccaagt ccgtgggcgt ggacgccgcg ttgctggtca cgccgtacta caacaagccc
     9181 acgcaggaag gcctgttccg gcattttgaa gccatcgcgc gcgcggtgga tctgcccttg
     9241 gtgttgtaca acattccggg ccggtccgtg gtcaacatga cgccgcagac cgtggcgcgt
     9301 ttgagcgcga tcaccaatat cgtgggcatc aaagaagcct ccgggaacct gtcgcagatg
     9361 agcgacgtga tcgccgcgtg cggggagcgg tttgtggtgc tctccggcga cgacgccttg
     9421 accctgccgc tcatggcggt gggcgggaag ggcgtgatct cggtgaccgc gaacgtggcg
     9481 cccgccgaga ccgcggacat ggtcagcgcc gcgctcaagg gcgattggga ccgggccaag
     9541 cgagtacact accgactgct gcccctgtcc aactccatgt tcctcgagac caacccgatc
     9601 ccggtgaaag ccgcgctcgc catgatgggc cggtgcgcgg acgaactgcg gttgccgctc
     9661 accacgatgt ccgacgcacc caaggcgcaa ctccgccagg cgcttctcca gttcggtctt
     9721 gtgtagcctg tccatgaatg gccgtctgcg gcgttgccgc tgcgcatccg gtgctcacgt
     9781 acccccctcg tacgctacgc tccggtgctc gcggcgcctt gcatccgaca cattcctgaa
     9841 caggctcgat cggccgtatc gccgattaac ttcgtagctc acacaacaaa tgttaaagtc
     9901 cccctccgtg aagccgaccc gcctcatcat catgggggcc ggggggaaga tggggcaacg
     9961 ccttgtcgcg ctcgcgacgg aaacgcccgg tcttgcgctc ggcgcggccg tggagcgcgc
    10021 gggccatcag gccgtgggcc gcgacgcggg cgaagccgcg ggcgtgggac gactggggat
    10081 cgcgatcagc gacgacgtaa agcgcgccct gtcggacggc gaggtggtga tcgacttcac
    10141 ctccccggcc gcgaccgccg cgttgatccc tctggcgctc gcgaccaagc ggccgctggt
    10201 gatcgggacc accgggatgt cccccgaggg attgcgcgca attcgggacg ccgcggccag
    10261 cctgccgatc gtgttcgcgc ccaatatgag tctcggcatg aacgtgatgt tcaagatcgt
    10321 ggccgacgtc gcgcgcgctc tcggccccgg ctacgacctg gagatcatcg aagccaaaca
    10381 tcgtctcaaa aaagacgcgc ccagcggcac cgcgctgcgg ctggccgaag tgctggccga
    10441 cgcgacgaaa cgccgtctcg acgaagtcgg cgtgtacgcg cggcacggcg agatcggggc
    10501 gcgcacgccc aatgagatcg gcatccagac catccgcgcc ggcgacatcg tgggcgacca
    10561 cacggtcctc ttcggcggcc tcggcgagcg gctggagatc acgcaccgcg cctccagccg
    10621 cgacacgttc gcgcgcgggg ccctgcgcgc ggcgcaatgg cttgccggaa aagctccggg
    10681 tctctacgac atgcaggacg tgctcggtct tcgttgagcc tgctcatcag gggccgtctg
    10741 cggcgttgcc gctgcgcatg cttcgccaag cgagcggaac acactattgc cagcgccaag
    10801 atgcggcgca gtgcgctttc cgctgctcac gtacgaccca gtatgctgcg ctgcgggtgc
    10861 tcgcggcgcc ttgcatccgg cccgctgctg cgcaggctga cggcgtggga gcgctccacg
    10921 ccggaactgc ggtcattccc gcgtaggcgg gaacttagcg cgttcgtggc gcttgagatc
    10981 ggtgatcttt cgtatccaag ttttatcccc cctttgaaaa aggggggaag gggggatttg
    11041 ccttgcgtct gacgatatat ccgctccgtc gattctctca cgggtagcct gatggaccta
    11101 ctcaccgatc ccaacgcctg gcttgcgctg atcacgctga cagccctcga aatcgtcctg
    11161 ggcatcgaca acatcatctt catctcgatc ctggcgggta aactgccgga agcccaacgc
    11221 gccagggccc gaaccatcgg cctcgctttc gcgatgggca cccgtctggc gttgctggcc
    11281 tcactggtgt ggatgatgcg tttgaccgct ccatggttca tcgtgcttga tcaggagatc
    11341 tcggggcgcg atctcatttt gattgccggc ggattgttcc tgctcgccaa gagcacgatg
    11401 gagatccacg acaagcttga aggggaagag gaatcgtcca gcggccgctc ggccacgacg
    11461 ttcgcaagcg tgatcgtcca aatcgcgatt ttggacatcg tgttctccct ggattccatc
    11521 atcaccgcga tcggtatggc caaccagttg ccggtcatgg tcgccgcggt gatcatcgcc
    11581 gtcattttca tgatgttctt ctccggcgcg gtcagcgcgt tcatcgaccg gcaccccacg
    11641 gtcaaggtgt tggcgttgag ttttttgttg atgatcggct tggcgctgat cggcgacggg
    11701 ttggacatgc atattccgaa gggctacatc tatttcgcga tggcgttttc ggtgttcgtc
    11761 gagatgctga atatccgggt caggaagaag gtggagccgg tgcatctgcg cggcccccac
    11821 gccccgtcga aatagtcagc cgtcgaacaa cgacgcctgc gcggtcgggc gccggaatgc
    11881 cgcggtggag agttccggcc agccggtgag gcccgcgcgt ttgcacgcca tcgcaaacaa
    11941 ggcgtggatc tggtccgcga acgggccttg gccggtcatg cgcgagccga agttggggtc
    12001 gttcagtgat ccgccgcgca ggtcacgcat gcggtggagc accttttctt tcatgccggg
    12061 gcgatgttga tccagccacg cttcgaatag cggcgccacg ccgtagggga ggcgcaaggg
    12121 caccaggccc gcgaatgttg cgcccgcgtc ggccgcggcc tgggtgatct tggggatttc
    12181 cgcgtcgttc agccccggaa tcacgggggc gacgttcacg cccaccggaa cgccggcctg
    12241 tgccagggct tcgatcgcgg ccaggcgttt tgccgggcgc gcggtgcgcg gctccaatgc
    12301 gccgcagagg tccgcgtcca gcgtggtgat cgacaacacc acggcgaccg cgtgatgctc
    12361 ggccaaccgc gacaagacgt ccgcgtcgcg cgtgaccagc gcgtttttgg tgatgatcac
    12421 caccggattg cgaaagtccg ccaggacttc aaggcactgc cgcgtcaatc gtagcttgcg
    12481 ctcgatgggt tggtagcagt ccgtcacccc gctcatggca atgagccgtg gggtccaact
    12541 ccgcttggac aattcacgac gcaacaactc cggcgcgtcg tgtttcacca tgatcttggt
    12601 ttcgaaatcc aatcccgcgg agaaccccaa gtactcgtgg gtgggtctgg cgtagcagta
    12661 cgcgcacccg tgctcgcacc cgcggtaggg attcacgccc gcatcgaacc ccacgtccgg
    12721 actgttgttg cgggcgatga tcgtcttcga tgcgtcggag agaaattggg tgaccggcaa
    12781 cggctgttca gcgtccgccg catcaatcgg ttcaggcacg cgctcaacgc cctcgaaccg
    12841 attcctggga tttcccgccg cgccgcggcc gtgtacgccg cgtggagtgg gatgttgcgg
    12901 gtccacctat cgcccccaga ttttctggaa ctcctcgggc gagtacccga cggtcacatg
    12961 cgttccgtcc gtcacaatcg gtcgtttgat cagccgaccc tcggaggcga gctgtttgat
    13021 gatggcgtct tccggcaatg ttttgacctt gtccttcatg ttgagcgcgc ggtactgcag
    13081 gccgctgcga ttcaagaagt cggtgtatgg ccggccgctc ctggcgatca gcccgcgcaa
    13141 ctcgtcggcc gatggcggcg acgtggtgac gtcgatgtcg cgaaaccggt gcccagcctt
    13201 ggcgagaaat ttcttggcgt tcacacacgt gctgcacttg gcatagccat agaacgtcaa
    13261 catgggagtg ctccggaagc ggaggggatg ctaccaagga gtcggtcgtc gatgcaacgc
    13321 acggccggga gcgtcgcgcg atagtgggtt ggcaaatcag attgcgctcg gtatactcct
    13381 gagcactttc cccaaccggg atgcccatga tgtttcctcc attcgtactg tggctgagtc
    13441 tcggcgtcgt tctgctcatc gtggaagtgg tgaccggggg cttttggatc ggtttcttcg
    13501 gcgtgggcgg tctggtcacg gcgctggcgc tctggaccga cgccattgcc ggactgaacg
    13561 cgcagctcgc ggtgttcctg atcgcgtccg ttctcccgtt ggtcgtactt cgcaagccgc
    13621 tcatggcgtg gctgaatcgg gacgtccaga ccagccgatt caacgacgcc gttggccaga
    13681 tcgcggtggt cgtgcaggac attgtcccgc ccggcgtggg acgggtagag tatcagggca
    13741 gcacgtggga ggcggactcg gccgcgggtg agcggttggc cgccaacacg cgcgtgcaaa
    13801 tcgttcgaca ggacggcacg cgcctgtcgg tgcgcgcgat ttcggaatga ccaagacggt
    13861 gtccgcacaa tttggtgtag agcctgttca ggaaaggtca gatgccaggc ggagggagga
    13921 ccttgcgcgc ggagcgtact gggtcgtacg ttagcacgca aggccgaccg acaacgccgc
    13981 agatggcctt tcatggacag gctcctagga ggacgacatg ctcgaatcca taagcccggc
    14041 ctggatggcg ctgatcgcgt tggcgatcat cttctacgcg ctggcgattc gcattgtgcc
    14101 ccaacagagc gccttcatcg ttgaacgtct gggccgctac
//
LOCUS       DBNGTT010000085         2152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1934897, whole genome shotgun sequence.
ACCESSION   DBNGTT010000085 DBNGTT010000000
VERSION     DBNGTT010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1934897"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /gene="pdxS"
                     /locus_tag="AC16BC_01985"
     CDS             <1..772
                     /gene="pdxS"
                     /locus_tag="AC16BC_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR3240413.1"
                     /translation="AVAVMALERVPADIRAQGGVARMADPKIIKGIMEAVTIPVMAKC
                     RIGHLAEARILQQLGVDFIDESEVLTPADEHYHVAKQAFTVPFVCGARDLGEALRRIG
                     EGAAMIRTKGEAGSGNVVEAVRHIRQLTDHMRRLTVAPPETLMAEAKALGAPYELLTD
                     VAKTGKLPVPNFAAGGVATPADAALLRLLGAEAVFVGSGIFKSRDPKARARAIVEATT
                     YFDQPEVLARVSEDLPGAMDGLDIRRLDEKELLQSRGW"
     gene            766..1359
                     /gene="pdxT"
                     /locus_tag="AC16BC_01990"
     CDS             766..1359
                     /gene="pdxT"
                     /locus_tag="AC16BC_01990"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIR3240414.1"
                     /translation="MVTSGSRRIGVLALQGDMDPHRAVLARVGADSVSVKTPSDLDGL
                     DGLILPGGESTAIGLLLVASGLDHAIREHVARRGLPLFGTCAGLILLARTIKETPNQP
                     RLALLDVVVQRNAFGRQQHSCEALVDAPRLGPEPLRALFIRAPLITETGPTVDVLGRW
                     NDRIVLVQQGPILAAAFHSELTDDDRVHRYFLGRTRA"
     gene            1549..>2152
                     /locus_tag="AC16BC_01995"
     CDS             1549..>2152
                     /locus_tag="AC16BC_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3240415.1"
                     /translation="MLVVNATPQLRLAFQDDQHGVTVTRKRLGQVPPTDGDVYGLIVA
                     ETPTKWEKDRTRIQRLLERSATGCMIIAPAPLLQRTVPAIQALGQAICAEPSQQTRTD
                     GDSTAAKIDQSSDGDFLQGYIERKLLKFVRAYSESGGRDLYGFMMKEFERPLLESALA
                     QTGGNQRRAADLLGMSRNTLRKRMQLCGIAQKSSTPRVRRR"
BASE COUNT          395 a          745 c          673 g          339 t
ORIGIN      
        1 ggcggttgcg gtcatggcgc tcgagcgcgt gcccgcggat atccgtgcgc agggcggcgt
       61 cgcgcgcatg gccgacccca aaatcatcaa gggcatcatg gaagcggtca cgattccggt
      121 catggccaag tgccggatcg gacacctcgc cgaagcccgg atcctccagc agttgggcgt
      181 ggatttcatc gacgagagcg aggtcctcac gccagccgac gagcactacc acgtggccaa
      241 acaggcgttc accgtgccgt tcgtgtgcgg cgcccgcgac ctcggagagg cgctgcggcg
      301 cattggagaa ggcgcggcca tgatccgcac caagggcgag gccggctcgg gcaacgtggt
      361 ggaagcggtc cgccacatcc gacaactcac ggatcacatg cgacggctca ccgtggcgcc
      421 gccggaaacc ctgatggccg aagccaaggc cctcggcgcg ccgtacgagc tcctgaccga
      481 cgtggcgaaa accggcaaac tccccgtgcc aaatttcgcc gccggcggcg tggccacgcc
      541 cgccgacgcc gcgttgctcc gcctcttggg cgcggaagcg gtgttcgtcg ggtccgggat
      601 cttcaagtca cgcgacccca aggcccgcgc gcgggcgatt gtcgaggcaa ccacctattt
      661 cgaccaaccc gaagtcctgg cgcgcgtgtc cgaagacctc ccgggcgcga tggacggcct
      721 ggatatccgt cgcctcgacg aaaaggaact gctccagagc cgcggatggt gacgagcggg
      781 tcgcgccgaa tcggcgtgct cgcgttgcag ggcgacatgg acccgcatcg cgcggttctt
      841 gcccgcgtgg gcgccgactc ggtgtccgtc aaaactccgt ccgacctgga cggactcgac
      901 ggcctgattc tcccgggcgg cgaaagcacc gcgatcggcc tgctcctggt cgcgagcggc
      961 ctcgatcacg ctatccgcga gcacgtcgct cgccgcgggc tcccgctctt cggcacgtgc
     1021 gcgggactga tcctgctcgc ccggacgatc aaagaaacgc cgaatcagcc ccggttggcc
     1081 ctgctcgatg tggtcgtaca acgcaacgcg ttcggccgac aacaacacag ctgcgaagcc
     1141 ctggttgacg ccccccgcct cggaccggaa ccgctccgcg cgttgtttat ccgcgcgccg
     1201 ttgatcacgg agaccggacc gaccgtcgac gtcctgggac ggtggaacga ccgcatcgtc
     1261 ctggtccaac aagggccgat cctggccgcc gcgttccatt cggaactgac ggacgacgat
     1321 cgggtgcacc gctattttct cgggcgcacg cgcgcctgac ccgtcgccgc ccgtccgcgc
     1381 ccccgcgatc gatcatccca tatcggaatg gttcgagccc gttgacagac tctttcgacg
     1441 tgtgatacaa ggtggccttc atttttggat cggacgcatt catttcccaa catgctcgct
     1501 cgtccccgac caaaatcagc tcctcgccgc gaggccaagc ccctccggat gttggtggtc
     1561 aacgccaccc cgcagttgcg actcgcgttc caagacgatc agcacggcgt cacggtgacg
     1621 aggaaacgac tgggccaggt tccgccgacc gatggagacg tctacgggct gatcgtcgcc
     1681 gaaaccccga ccaaatggga aaaggaccgg acccgcatcc aacgcctgct cgaacgatca
     1741 gcgaccgggt gtatgatcat tgcgcccgcc ccgctgctgc aacgcacggt gccggccatt
     1801 caggcgttgg ggcaagccat ttgcgcggag ccttcgcagc agacgagaac cgatggcgac
     1861 tccaccgccg ccaagatcga ccaatcatcc gacggtgatt ttctccaagg ctacatcgag
     1921 cgtaaactgc tgaagttcgt cagggcctac agcgaaagcg gcggccggga cctgtacgga
     1981 ttcatgatga aggaattcga gcgccccctg ctcgaatccg cgttggccca gaccgggggg
     2041 aatcagcgac gcgccgcgga cctgctcggg atgagccgca acacgctccg aaaacgtatg
     2101 cagctctgcg gcatcgcgca gaaatcctca acgcccaggg ttcgtcgccg ca
//
LOCUS       DBNGTT010000086         6168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2318194, whole genome shotgun sequence.
ACCESSION   DBNGTT010000086 DBNGTT010000000
VERSION     DBNGTT010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2318194"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /gene="polX"
                     /locus_tag="AC16BC_02000"
     CDS             <1..1549
                     /gene="polX"
                     /locus_tag="AC16BC_02000"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR3240416.1"
                     /translation="IPGVGTHTADKIAALLDTGRLPYYDELRAKTPVDLAGLSAIEGL
                     GPKMIKALYDQLGVKTVDDLEAAAQAGKVRRLPHFGEKSEQKILKGIAFVKQRAGRVP
                     LGDAWPLMHELAERLRAVRGVARIEVAGSIRRRRETIGDGDLLAVSHEPERVMDALAG
                     LPEVADVIAHGPAKTSVKLRSGMDVDMRVVPAESFGAALLYFTGSKAHNIALRKLAIA
                     KGLKLSEYGVFRGKRAVAGLTEEDVYAALGLPYIPPELREDAGEVEAGLAGTLPDLIG
                     YDDLRGDLQTQTDWTDGADSIAAMAAAARRRGLEYIAITDHTKGLAMTNGSDEKKLRK
                     QMAAIDALNAEGPGIAILKGAEVNINKDGTLDIDDATLAMLDVVGVAVHAHFHLPRAE
                     QTRRVIRAMENPHADILFHPTGRLIGKREAYDIDIDAVIAAAKRTGTVLEIDAFPDRM
                     DLKDDHARKAASAGVKLVIDSDAHATGHFQVLHYGIAAARRGWVTKADVINTRPLREF
                     LRALKGA"
     gene            complement(1563..2327)
                     /locus_tag="AC16BC_02005"
     CDS             complement(1563..2327)
                     /locus_tag="AC16BC_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3240417.1"
                     /translation="MYDCREIRQLVHASLDRELDVTASLLVQDHVAWCGACREILLDE
                     QTFLTLTTIVLEPPRAPDRTRSAVRETLSREVASVQRTRRRRHGLLSRGTLAAGLVLA
                     VFFSVPHMRVPDLVKIAVAEHRLYLQDPARLPLATSDVREAQHWLDRRLPFSARIPDR
                     TTPRARLVGASARTGPNASAILTYTWDGMPISLLIAPARTVQFSGADAVTFRNVVFQT
                     ALVESRRVLRWSDDRHTYVMVACGDVPIESLPFAVP"
     regulatory      complement(2423..2503)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2688..3602
                     /locus_tag="AC16BC_02010"
     CDS             2688..3602
                     /locus_tag="AC16BC_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR3240418.1"
                     /translation="MLSVLAQTAALIAVGVVWRAVRPWGLDPDVTRRVLTGLVYVLLL
                     PALVLDVLWRAPLGLDAGRTAASAAVGVFAGLAAAWAWTRVRRMPSAERGALLLAAAF
                     GNVTYLGLPVLESAFGGWARTVAIEYDLFACTPLLLTVGIVIARAHGTGVDDEHLLAG
                     LAKVPALWAALVAVGLNAFDVPTPATVGDALSRLGGGVMPLMLISIGLALQWERDWRG
                     RVPAVLPVLAIRFAIAPLMVWGAAAASGLEGDVLGAVVLEAAMPSMVLGVVICDRYGL
                     DTAWYAAAVTFSTAASVVALPLWHAIVA"
     gene            3694..4641
                     /locus_tag="AC16BC_02015"
     CDS             3694..4641
                     /locus_tag="AC16BC_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3240419.1"
                     /translation="MSSAKTGMSRCGIVLAGGDGRRLEEFVRRLRGDALPKQYVDFTG
                     QGSLLEQTFQRAERLIPARRLFTVVTRSHFAFPEVWRQLADRPGGRVVVQPENKDTAP
                     GLLLPLVRLVRECGDSTVAVFPSDHAIEDDNAFMSYVEQAYEAVERRSSRIVLLGVSP
                     TAPEADYGYVVSGEATSDGLRAVTRFVEKPGTTRANELIERGALWNTFVMVFRAGTLL
                     AYLRDAAPTLVGAFDRLALARTERAERAELDEVYQRIQPLNFSSAFLRRLPGAAHPAL
                     TVLAMDGVGWNDCGVEARLPHAVRDAVREPIAEPVPLCA"
     gene            4908..5711
                     /locus_tag="AC16BC_02020"
     CDS             4908..5711
                     /locus_tag="AC16BC_02020"
                     /inference="COORDINATES: protein motif:HMM:NF025670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HIR3240420.1"
                     /translation="MRCKRLSDLRIFPELRGFVGRGGVLVLILWATVACNGDGPASGS
                     GPTLSVGFGLKQLQLTWTAVADADYYRLLESLDSGASFSQLGGQIAAGTTSQTIDIAV
                     HRHDWANARYKLEACSTSGCIASNTVATLDGVLRTIGYVKASNTETEDGFGSAVAVSS
                     DGNTLAVGATGEDSSATGIDGTAGNNGALNAGAVYVYTRDTAGAWSQQAYVKASNTET
                     EDGFGGAVAVSSDGNTLAVGATGEDSSATGIGGTAGNNGALNAGAVYLY"
     gene            5923..>6168
                     /locus_tag="AC16BC_02025"
     CDS             5923..>6168
                     /locus_tag="AC16BC_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HIR3240421.1"
                     /translation="MTAKEVLEYAKKNKAVMVDLKFVDLPGIWQHFSIPIEELSEGIF
                     KEGLGFDGSSIRGWQAINNSDMLVMPDPGTAVMDPFMK"
BASE COUNT         1009 a         1881 c         2247 g         1031 t
ORIGIN      
        1 gattcccggg gtcgggacgc ataccgcgga caagatcgcc gccctgctcg acaccggccg
       61 gctcccgtac tacgacgaac tccgggccaa gacgccggtg gacctcgccg gcctgtccgc
      121 gatcgagggg ctggggccca agatgatcaa ggcgctctac gaccagctcg gggtgaagac
      181 cgtggacgac ctggaggccg cggcccaggc cgggaaggtc agacgcctcc cgcacttcgg
      241 ggagaagagc gagcagaaga tcctcaaggg catcgcgttc gtgaaacagc gcgccggacg
      301 cgtcccgctg ggcgacgcgt ggccgctgat gcacgaactg gccgaacgcc tgcgcgcggt
      361 gcgcggggtg gctcgcatcg aggtggcggg gtcgatcagg cgtcgccggg agaccatcgg
      421 cgacggcgat ctcctggcgg tctcccacga gcccgaacgc gtgatggacg cgctggcggg
      481 gttgcccgag gtggcggacg tgatcgcgca cgggcccgcc aagaccagcg tgaagctgcg
      541 ctcgggtatg gacgtggaca tgcgcgtggt cccggccgag agtttcggcg cggcgctgct
      601 gtatttcacc gggagcaagg cgcacaatat cgcgttgcgc aagctggcga tcgccaaggg
      661 cctgaaactc tccgagtacg gcgtatttcg cggcaagcgc gcggtcgcgg ggcttaccga
      721 ggaggacgtg tacgcggcgc tcggcctgcc gtacatcccg ccggagttgc gcgaggacgc
      781 gggggaagtc gaagccgggc tggcgggcac gttgcccgac ctgatcgggt acgacgacct
      841 gcggggcgac ctccagaccc agacggactg gaccgacggc gcggacagca tcgcggcgat
      901 ggcggccgcg gccagacgcc ggggcctcga gtacatcgcg atcaccgacc ataccaaggg
      961 cctggccatg acgaacggct cggacgagaa aaaactccgc aaacagatgg ccgcgatcga
     1021 cgcgttgaac gccgagggcc ccggcatcgc gatcctgaag ggcgcggagg tcaacatcaa
     1081 caaggacggc accctggaca tcgacgacgc cacgctggcc atgctcgacg tggtgggggt
     1141 ggcggtgcac gcgcactttc acctcccgcg cgcggagcag acgcgccgcg tcatccgcgc
     1201 catggagaac ccgcacgcgg acatcctgtt tcacccgacc ggccggctca tcggcaagcg
     1261 ggaggcgtac gacatcgaca tcgacgcggt catcgcggcc gccaagcgga ccgggacggt
     1321 gttggagatc gacgcgtttc cggaccggat ggacttaaag gacgaccacg cgcgcaaagc
     1381 cgcgtccgcg ggcgtcaaac tcgtgatcga ctcggacgcg cacgcgaccg gccatttcca
     1441 ggtcctgcac tacggcatcg cggcggcgcg gcggggctgg gtgacgaaag ccgacgtgat
     1501 caacacgcga ccgctccggg aattcctgcg cgcgctcaag ggcgcgtaac gagccgcgcg
     1561 gtttacggca cggcaaacgg cagcgactcg atcgggacgt cgccgcaggc gaccatgacg
     1621 taggtatgtc ggtcgtccga ccaccgcagg acccggcgac tttcaacgag ggccgtctgg
     1681 aacacgacgt ttcggaacgt gaccgcatcc gcgccgctga attggacggt ccgcgcgggg
     1741 gcgatcagca gcgagatcgg catgccgtcc cacgtgtagg tcaggatcgc ggaggcgttc
     1801 ggtccggtcc gcgcggaggc gccgaccaga cgcgcgcgcg gcgtggtccg gtccggaatg
     1861 cgggcggaga aagggagccg ccggtcgagc cagtgttggg cctcgcgaac atcggaggtg
     1921 gcgagcggaa gccgcgcggg atcttgcagg tagagccggt gttcggccac ggcgattttc
     1981 accaggtcgg ggactcgcat gtgtggcacg gagaaaaaga ccgccaagac caggccggcc
     2041 gccagcgtgc cgcgggacag gagtccgtgt cgacggcgtc gcgttcgttg cacgctcgcg
     2101 acctcccgcg acagggtttc ccgaaccgcc gaccgcgtgc gatccggcgc gcgcggcggc
     2161 tcaagaacga tggtggtcag ggtgagaaac gtctgttcgt cgagcaggat ctcgcggcac
     2221 gccccgcacc aggccacgtg atcctggacg agcagcgacg ccgtgacgtc cagttcccgg
     2281 tcaagggacg cgtggacgag ttgtcgaatc tccctgcagt cgtacaccgc gagcccccct
     2341 ctcgagatgt cgaatgcgaa agcccgatac aatacagccg ggcttccgac atggcaataa
     2401 tcgtggtctt gattattgtc acctcggcgg cccggctgtc tttgatcagc aaagatccgg
     2461 tgctttgcgt cctaccgtcg ccggtagttt gccctgatgg aaggtggttg ctcgtgtgtc
     2521 cgcgcgatta gcctctcgag tgagcgaatg cctcgtaatt ttgctcatgc tcataacacg
     2581 atggagttag gcgtgtcaag cgatcagttc gcggtctgca ctcgcgttcc gcgacgtgca
     2641 tcgggtgatc ccgtgcgcgc ggcgatgacg cgcccgcctg ggcgatcgtg ctgagcgtgc
     2701 tcgcgcagac cgccgcgttg atcgcggtcg gcgtggtctg gcgggcggtc aggccgtggg
     2761 gcctggatcc ggacgtgacc cggcgggtgc tcaccggcct ggtctatgtg ttgctgctgc
     2821 cggcgctggt gttggacgtg ttgtggcggg cgccgctcgg cctcgacgcg gggcgcaccg
     2881 cggcctccgc cgcggtcggg gtatttgcgg ggctggccgc ggcctgggcg tggacgcggg
     2941 tccgacggat gccgtcggcc gaacggggag ccttgttgtt ggccgcggca ttcgggaacg
     3001 tgacgtatct cggactgccc gtgctggaaa gcgcgttcgg cgggtgggcg cggaccgtgg
     3061 ccatcgagta cgacctgttc gcgtgtacgc cgttgttgct gaccgtgggc atcgtgatcg
     3121 cgcgcgccca cggcacgggc gtcgacgacg agcacctgct cgcggggttg gccaaggtgc
     3181 cggcgttatg ggccgcgctc gtcgcggtgg gactcaacgc gttcgacgtc ccgacacccg
     3241 ccacggtcgg ggacgccttg agtcggttgg gcggcggggt catgcccttg atgctgatct
     3301 cgatcgggtt ggcgctccaa tgggagcgcg actggcgtgg tcgcgtgccc gcggtactgc
     3361 cggtgttggc gatccggttc gcgatcgcgc cgctcatggt gtggggcgcg gccgccgcgt
     3421 ctgggttgga gggcgacgtg ttgggcgcgg tggtgctgga ggcggccatg ccgagcatgg
     3481 tgttgggcgt ggtgatctgc gatcggtacg gcttggacac cgcgtggtac gcggcggccg
     3541 tcacgttctc cacggcggcg agcgtggtcg cgttgccgct gtggcacgcg atcgtcgcgt
     3601 gagtttacag aaatcccacc ctcggctatt ctgaaccgct gtcgtcctcg gatcatcgtt
     3661 cgggttgatt ccatatctta taaaggagga ggcatgagca gcgcgaagac agggatgtct
     3721 cggtgcggca ttgtgttggc cggcggcgac ggccgtcgat tggaggagtt cgtgcggcgg
     3781 ctgcgcggcg acgcgttgcc caagcaatac gtggatttca cggggcaagg atcattgctc
     3841 gaacagacgt ttcagcgcgc cgagcggctg attcccgcgc ggcggctgtt cacggtcgtc
     3901 acccgcagcc acttcgcgtt tcccgaagtc tggcgacagt tggcggatcg cccgggcgga
     3961 cgggtggtgg tccaaccgga aaacaaggat acggcccccg gcctgctcct gccgctcgtg
     4021 cgtctcgtgc gcgaatgcgg cgactccacg gtggcggtgt ttccctcgga ccacgcgatc
     4081 gaggacgaca acgcgttcat gtcgtacgtt gagcaggcct atgaggcggt cgagcgccgg
     4141 tcgtcgcgca tcgttctgct cggggtttcg cccaccgcgc ccgaagcgga ctacggctac
     4201 gtggtctccg gcgaggcgac cagcgacggc ctgcgcgcgg tgacccggtt cgtggaaaag
     4261 ccgggcacga cccgcgcgaa cgaactgatc gagcgcggcg cgctgtggaa cacgttcgtg
     4321 atggtgtttc gcgccggcac gctgttggcg tatctgcgcg acgcggcgcc gaccctcgtg
     4381 ggggccttcg atcgcctcgc gctggcgcgg accgaaagag cggagcgcgc ggagctcgac
     4441 gaggtgtatc agcgcatcca gccgctcaat ttctcgtccg cgttcctgcg tcgtcttccc
     4501 ggcgcggcgc accccgcgct cacggtcctc gcgatggacg gcgtgggctg gaacgactgc
     4561 ggcgtggaag cccgcctgcc ccacgcggtc cgcgacgcgg tccgcgaacc gatcgccgag
     4621 ccggttccgc tctgcgcctg atcgaaggac gttaaatccg gcacgcgtca atcgcgctgc
     4681 tgatctccgc gattgacgcg tccttgccat ttggaagcta ttcccgacgc tccgtgcacg
     4741 agggcggccg gggaaggaac tgtgatacat ggttcctgac ccctgccccg tcgcattgcg
     4801 agggcctcgt caaggtgctc gcagatcatc ggaccgattg actccaactt cgagtttggg
     4861 gcaaactggc catagttcat ggatcgtcgt gacttggaga ccttctcatg cgctgtaagc
     4921 gcttgtccga cttgcggatt ttccccgagc tccgtggttt cgttggccgc ggaggcgtgc
     4981 tggtcctgat cctgtgggca actgtggcgt gtaacggcga cgggcccgct tcagggtcag
     5041 gaccgaccct cagcgtaggc ttcggcctca agcagctgca gcttacgtgg accgccgtcg
     5101 cagacgcgga ctactaccgt ctcttggaga gcctcgacag cggcgcgagt ttctcccagc
     5161 tcggaggtca gatcgcggcg ggaactacca gccagactat agacatcgcg gtgcatcgtc
     5221 atgactgggc gaacgcccgg tataagctgg aggcttgcag caccagcggc tgtatcgctt
     5281 cgaacaccgt tgccacgctg gatggcgtgc tccggacgat cggctatgtg aaggcctcga
     5341 acacggagac ggaggatggg ttcgggagcg cggtggcggt gagcagcgac gggaacacgc
     5401 tggcggtggg cgccacgggc gaggacagca gcgcgacggg gatcgacggg acggcgggca
     5461 ataacggcgc gctcaacgcg ggagcggtgt acgtgtacac gcgggacact gccggcgcgt
     5521 ggagccagca agcgtacgtg aaggcctcga acacggagac ggaggatggg ttcgggggcg
     5581 cggtggcggt gagcagcgac gggaacacgc tggcggtggg cgccacgggc gaggacagca
     5641 gcgcgacggg gatcggcggg acggcgggca ataacggggc gctcaacgcg ggagcggtgt
     5701 acctctattg acacccgcga ccgaagcggg ggggccgttg ccgccggtcg cttgacactc
     5761 gtctttccca cgtgatataa gcggcgcacg tgtccggatt ttccggccgg aaccaggagg
     5821 acaaagacgt ccttcaggcg tcggtatgcg cctggggcgt cttttttgtt ttagaggcgg
     5881 tgcgcgcgta gcggggcaga ttgaatcaca aaggagaggg caatgacggc gaaggaagtc
     5941 ttggagtacg cgaagaagaa caaggcggtg atggtcgatc tgaaattcgt cgaccttcct
     6001 ggcatctggc agcacttttc cattccgatc gaagaactga gcgaagggat cttcaaggaa
     6061 gggttggggt ttgacgggtc gtcgatccgc ggctggcagg ccatcaacaa cagcgacatg
     6121 ttggtgatgc cggatccggg gaccgcggtg atggatccgt tcatgaag
//
LOCUS       DBNGTT010000087         2873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_274077, whole genome shotgun sequence.
ACCESSION   DBNGTT010000087 DBNGTT010000000
VERSION     DBNGTT010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_274077"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..>2873
                     /locus_tag="AC16BC_02030"
     CDS             100..>2873
                     /locus_tag="AC16BC_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR3240422.1"
                     /translation="MTDRDLEPEIHRIGRDILDRVGERRAAVFDPSRWAGQIMDRVIR
                     DEPFKLALFRFVDVLPALSDSADAAAVFAQYMKGLSLPAVFSWGLTHLESDSLAGRLA
                     GRALQEQVRALASLFIAADAIDRVGPSLAALRASGRGATVALLGETALSEPEADAYRD
                     RYHDAIAAVAEASRETRAFAASHPLARVPTANVSIKASSLDARLDPADRDGSLARLAG
                     RLGPIMTAARRAGAHAHLDMEHFALKDLLLALAERLFARGDGAGADAGLVIQAYLRDA
                     ETDLHTVLGWASRRPRPLTIRLVKGAYWDAEVMTARQKGWPTPVLTDKAATDAQFERL
                     TRVLLDHAPAVRPAIGSHNVRSLAAAIAYAERLGLPRSAYELQLLYGMGDPIAETLAD
                     MGYGVRVYAPVGELIPGMAYLVRRLLENSANESFLRRRFQDRQDADELLKPPIMPLVG
                     TTGRSPRPDGHRVPPGEAFSNEPPLDFSRNDAREAMRTAITRARETLGHSYPLIIDGR
                     RVETRRTATSVNPAAPDEIIGHVAQAGHDQADPAVAGAAHALTTWRAVPVPERAALLQ
                     RVAERLRASRFDLAALEVLEVGKSWREADADVCEAVDFLEYYAREMLRLAPPRRMSDV
                     PGEDNLYWYVPRGVGVVVAPWNFPLAIPAGMIAAALVAGNVVIFKPSGLSPVLGARLV
                     DAFTAAGLPPGALQFLPGPGSEIGDLLVDHPLVDFITFTGSKEVGLRILERAARTPPG
                     QRRVKRVIAEMGGKNAVVVDRTADPDEAIAGILTSAFGYQGQKCSACSRVIVEEAIAG
                     TLIARLTRAIESLAVGPPEDPANRVGPVINAAARDRIQATIARGVADGLALRVAHPPP
                     LPGYYVAPALFGPVPAHHPLAQEEIFGPVLSILTARNFDHALALANDVEFALTGGLYS
                     RSPANIRTAG"
BASE COUNT          410 a         1046 c          979 g          438 t
ORIGIN      
        1 acttgttcga aggggtccag cgcggcgata tcgtggacag caagactctg ctcgccgcac
       61 tgtggtatcg caccttccgt tcgcagagcc ggtgacgccg tgacggaccg ggatctcgaa
      121 ccggagatcc accgcatcgg ccgcgacatc ctggaccggg tgggcgaacg gcgcgccgcg
      181 gtgttcgacc cttcccggtg ggcgggacag atcatggacc gggtgatccg ggacgagccg
      241 ttcaagctcg ccctgtttcg cttcgtcgac gtcctcccgg cgttgtccga ctccgctgac
      301 gcggccgcgg tgttcgcgca gtacatgaaa ggactctcgc tccccgcggt tttctcgtgg
      361 ggactcacgc atctcgaatc ggactccctc gccggtcggt tggccggccg cgccctccag
      421 gaacaggtcc gggcattggc ctcgctgttc atcgcggccg acgccatcga ccgcgtgggg
      481 ccttcgctgg ccgcgctccg ggccagcggc cgaggcgcga ccgtcgcgtt attgggcgag
      541 accgcactca gcgagccaga ggccgacgcc taccgcgacc ggtaccacga tgcgatcgcg
      601 gcggtcgcgg aggcctcccg cgaaacgcgc gccttcgccg cgtcccaccc cttggcccgc
      661 gtcccgacgg ccaacgtgtc gatcaaggcc tcgtcgctcg acgcgcgtct cgatccggcg
      721 gatcgcgacg gcagcctggc gcgactcgcc ggtcgcctgg gaccgatcat gacggccgcg
      781 cgccgggccg gggcgcacgc gcacctcgac atggaacact tcgcgctcaa agacctgctc
      841 ctggccctgg ccgagcgcct gttcgcgcgc ggcgacggcg ccggcgccga cgccggcctg
      901 gtgatccagg cgtacctgcg cgacgcggaa acggatttgc acacggtgct cgggtgggca
      961 tcgcgccgac cgcgtccgct cacgattcgt ttggtcaagg gcgcgtattg ggacgcggag
     1021 gtcatgaccg cgcggcaaaa gggctggccg acccccgtgc tgaccgacaa ggccgccacc
     1081 gacgcgcagt tcgagcgtct cacccgggtc ctgttggacc acgcccccgc cgtccgtccc
     1141 gcgatcggct cgcacaacgt gcgcagcctc gcggcggcca tcgcgtacgc cgaacgattg
     1201 gggctgccgc gctcggcgta cgaactgcag ctcctctacg gcatgggcga tccgatcgcc
     1261 gagaccttgg ccgacatggg ctacggggtc cgcgtctacg cgccggtggg agaactgatc
     1321 cccggcatgg cgtacctggt ccgccggttg ctggagaaca gcgcgaacga gtccttcttg
     1381 cgccggcggt tccaggaccg acaagacgcc gatgaactgc tgaaaccgcc gatcatgccc
     1441 ctcgtgggga cgaccggccg ctcgccccga ccggatggac accgcgtccc gcccggcgag
     1501 gccttcagca acgagccgcc gctggatttc tcccggaacg acgcccggga ggcgatgcgg
     1561 accgcgatca cccgcgcgcg cgagaccctg ggacactcgt acccgctgat catcgacggc
     1621 cgccgcgtgg agacccgccg caccgcaacc tccgtcaacc ccgcggcgcc ggacgagatt
     1681 atcggccacg tggcgcaagc gggacacgat caggccgatc cggccgtggc cggcgccgcc
     1741 cacgcgttga ccacgtggcg cgcggtcccg gtgcctgagc gggcggccct gttgcaacga
     1801 gtcgcggagc gcctccgggc gtcgcggttc gacttggccg cgcttgaggt gttggaggtc
     1861 ggtaaaagct ggcgcgaagc cgacgcggac gtctgcgaag ccgtcgactt tctcgagtac
     1921 tacgcgaggg agatgctccg ccttgcgccc cccaggcgga tgagcgacgt tcccggcgag
     1981 gacaacctgt attggtacgt gccccgcggc gtaggggtcg tggtcgcgcc gtggaatttc
     2041 ccgctcgcaa ttcccgccgg catgatcgca gccgcgctcg tcgccgggaa cgtcgtcatc
     2101 ttcaaaccct cggggttgtc gcccgtgctc ggcgcgcgcc tggttgacgc gttcaccgcg
     2161 gcaggccttc cgcctggcgc gttgcagttt ctgccgggcc ccggaagcga gatcggcgac
     2221 ttgctggtcg atcacccgct cgtcgacttc atcaccttta ccgggtccaa agaggtgggc
     2281 ctgcgcatcc tcgagcgcgc ggcgcgcacc ccgcccggtc agcgccgcgt caaacgcgtc
     2341 atcgccgaaa tgggcggcaa aaacgcggtg gtcgtggacc gcaccgccga ccccgatgaa
     2401 gccatcgcgg ggatcctgac gtccgcgttc ggctatcaag gccagaagtg ttcggcctgt
     2461 tcccgcgtga tcgtcgagga ggcgatcgcg gggacactga tcgcccggtt gacgcgcgcg
     2521 atcgagagcc tggccgtcgg cccgccggag gaccccgcga accgcgtcgg accggtcatc
     2581 aacgcggcgg cccgcgaccg catccaggcg acgatcgcgc gcggcgtggc cgacggcctg
     2641 gccctgcgcg tcgcgcaccc accgccgctc ccgggctact acgtcgcgcc ggccctgttc
     2701 ggtccggtcc cggcgcacca cccgttggcg caggaggaga tcttcgggcc cgtgctgtcg
     2761 attctcacgg cgcggaactt cgaccatgcg ctcgccctgg cgaacgacgt cgagttcgcg
     2821 ttgaccggcg gtctgtactc gcgcagcccg gccaacattc ggaccgccgg cga
//
LOCUS       DBNGTT010000088         2922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1423910, whole genome shotgun sequence.
ACCESSION   DBNGTT010000088 DBNGTT010000000
VERSION     DBNGTT010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1423910"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="AC16BC_02035"
     CDS             <1..915
                     /locus_tag="AC16BC_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR3240423.1"
                     /translation="YIFLKDKYQEEHRVLGAALEAARQKGYWGPSVLGSSFGVTVHLF
                     RGPDAYIAGEETAMLEAIEGKTPRPRQKPPYYPIQYGLFGLPTLVNNVETLANIPKII
                     LRGGAWFAAVGLPTSPGTMLFSLTGAVARPGVYELPLGTPLRSLIYDVGGGIAGGRSL
                     KAVFPGGPSQTVLTGKDLDVTLDFDSLKKIGSGLGTAGVMVYDDQTCMLRLAIEFARF
                     YQEGSCRQCPPCELGTQNIAKLVSKIEEGQAEPKDLDFLTQVFGLVRGRGYCDLISSA
                     VRSVESTVKVFRDEYEAHITGHGCPYPR"
     gene            1084..1407
                     /gene="erpA"
                     /locus_tag="AC16BC_02040"
     CDS             1084..1407
                     /gene="erpA"
                     /locus_tag="AC16BC_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR3240424.1"
                     /translation="MVVITEKAGAKIKDLLAGEEKPSQGLRIYVSGGGCQGFQYGMAF
                     EEQANADDTVIEAHGVRVFVDAQSAPLLSGAEVDYIDSLEGSGFSIKNPQAKSSCGCG
                     QSFNA"
     gene            1422..1589
                     /locus_tag="AC16BC_02045"
     CDS             1422..1589
                     /locus_tag="AC16BC_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240425.1"
                     /translation="MASARYQPEWNSTEAVLADRLRADADEFCTVAWREWLTAMVVVE
                     MARSETRVSWH"
     gene            1702..2049
                     /locus_tag="AC16BC_02050"
     CDS             1702..2049
                     /locus_tag="AC16BC_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3240426.1"
                     /translation="MSLRITEECISCGACLPECPNEAIFDSRASAESAGWHISEGDGM
                     ADGLYVITYDRCTECVGHYDAPQCAAVCPVPDCCISDPQYPEAKDVLLDKARKLNPGK
                     EIDSNKVWSGVRG"
     gene            2128..2775
                     /locus_tag="AC16BC_02055"
     CDS             2128..2775
                     /locus_tag="AC16BC_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240427.1"
                     /translation="MDTFSREEIDFLKQAGLFAWKERLTRRLQTELTALHEALVSRVV
                     PDAILAPDEIDWTRWQLVRGERFHDRPYAYLDYPQYFARATKFAYRSMFWWGEGVFFA
                     MILEGALLDRYVERLDRAYAEIADRDLSVSLADTPWEWARAGRAVLPIRSDTKDAVLA
                     AARSRPFLKLQRAIALDRLVEPDAIVREGAATFDALVPVIARKTFPGNHGGRGGI"
     gene            complement(2772..2921)
                     /locus_tag="AC16BC_02060"
     CDS             complement(2772..2921)
                     /locus_tag="AC16BC_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3240428.1"
                     /translation="MTLDIKKSAVEANALMSERAIRHLVITEEGKIVGVLSVRDLLVY
                     FKNQF"
BASE COUNT          528 a          830 c         1019 g          545 t
ORIGIN      
        1 tacatctttc tcaaggacaa atatcaggag gagcaccggg ttctcggcgc ggcgctggag
       61 gcggcgcgcc agaaaggcta ctgggggccc tcggtcctcg gctcctcgtt cggcgtgacc
      121 gtgcatctgt ttcgcgggcc cgacgcgtat atcgcgggcg aagaaaccgc gatgttggag
      181 gccatcgagg gcaagacccc gcgcccgcgg cagaagccgc cgtattaccc gatccaatac
      241 gggctgttcg gattgcccac attggtcaac aacgtggaga cactggccaa tatccccaag
      301 atcatcttgc gcggcggggc atggtttgcc gcggtgggat tgcccacgag tccgggcacc
      361 atgctgttca gcctgaccgg cgcggtggcg cggcccggcg tgtacgagtt gccgttgggc
      421 acgccgttgc gctcgctgat ctacgacgtg ggcggcggga tcgcgggcgg gcgctcgttg
      481 aaggccgtgt tccccggcgg accgtcgcag accgtgctga cgggcaagga tctggacgtg
      541 acgctcgact tcgactcgct caaaaaaatc gggtcgggcc ttgggaccgc gggcgtgatg
      601 gtgtacgatg atcagacgtg tatgctgcgg ctggccatcg aatttgcgcg attttatcag
      661 gagggctcgt gccgccagtg tccgccgtgc gagttgggca cgcagaatat cgccaaactc
      721 gtgtccaaga tcgaagaggg gcaggccgag cccaaggact tggattttct gacccaggtt
      781 ttcggcctgg tccggggacg cgggtattgc gacctgatca gcagcgcggt gcgctcggtg
      841 gagagcacgg tgaaagtgtt tcgcgacgag tacgaagccc atatcacggg gcacggctgt
      901 ccgtaccccc gatgaccggc gcgcccaaag gccctatggg gcgtgggcgc gtggtccagg
      961 tggcctaggg ctccgcgctc attgacaagg tttccggcgc gatgctacga tacgcaagct
     1021 cggtacagat aaacattttc tgctgacgtt tcaactagtt cggcggagac aaggagtcac
     1081 gatatggtgg tcatcacgga aaaagcgggc gcaaaaatca aagatctcct ggccggcgaa
     1141 gagaaaccaa gccaggggtt gcgaatctac gtgtccggcg gcggctgtca gggatttcag
     1201 tacgggatgg cgttcgagga gcaggccaac gcggatgaca cggtcatcga ggcccacggg
     1261 gtccgcgtgt tcgtggatgc ccagagcgcg cccttgttga gcggcgcgga agtcgactac
     1321 atcgacagcc tcgaaggctc ggggttttcg atcaagaacc ctcaagccaa gtcgtcgtgc
     1381 ggctgcgggc agtccttcaa cgcgtaagcc gttccggcac catggcgagc gctcgatatc
     1441 aaccggagtg gaacagcacg gaggcggtgt tggcggatcg cttgcgcgcc gatgccgacg
     1501 agttttgcac cgtggcttgg cgggaatggc tcacggcgat ggtggtggtg gagatggcgc
     1561 ggtcggaaac ccgcgtctcg tggcactaat cgaatcatcg ttggacggcg ccgcttccca
     1621 gtgagcggtg ccagcgagta aggccctcgg cggcaggaga cgccgcgagt cggcgtgacg
     1681 acaacagaag gaggagagcg tatgtcgctc aggattactg aagagtgtat ttcatgcgga
     1741 gcgtgcctgc cggagtgtcc gaacgaggcg attttcgaca gccgggcgtc ggccgaatcc
     1801 gcggggtggc acatctcgga gggagacggg atggccgacg gcctgtacgt gattacctac
     1861 gatcgttgca ccgagtgcgt gggccattac gatgccccgc agtgcgcggc cgtgtgtccg
     1921 gtgcctgact gctgcatctc cgacccgcag tatcctgaag ccaaggatgt cctgctcgac
     1981 aaggcgcgga agttgaatcc gggcaaagag atcgactcga ataaggtctg gtccggcgtg
     2041 cgcggataac cggatctgag aacttgtgag gggccgggcg gacgcgttcc gcccggcccc
     2101 tcgttgtttg aggcggacgg agcggccatg gacacgttct cccgcgagga gatcgacttt
     2161 ctcaagcagg cgggactgtt cgcgtggaag gaacgcctga cgcgccgtct ccagaccgag
     2221 ttgaccgcgc tgcacgaggc gctcgtttcg cgcgttgtcc ccgacgcgat cctggcgccc
     2281 gacgaaatcg actggacgcg ctggcaactc gtgcgcggcg agcgattcca cgatcgccca
     2341 tacgcctatc tcgactaccc gcagtacttt gcccgcgcga cgaagttcgc ttaccggtcg
     2401 atgttctggt ggggtgaagg cgtgttcttc gccatgattc ttgaaggcgc gttgctcgat
     2461 cggtacgtcg agcgtctcga ccgggcgtac gcggagatcg cggatcgcga tctgtcggtg
     2521 tcattggcgg acacgccatg ggagtgggcg cgagccggac gcgcggtgtt gccgatccga
     2581 tccgacacca aggacgcggt gttggccgcg gctcggagcc gaccatttct gaagctccag
     2641 cgggcgatcg cgctggatcg gttggtcgaa cccgacgcga tcgtgcgcga gggcgcggcg
     2701 acgtttgacg ctctggtgcc ggtgatcgcc aggaaaacct tccccggtaa tcatgggggg
     2761 cgagggggga tttagaactg gttcttgaag tagacgagca ggtcgcggac cgagagcacg
     2821 cccacaattt tgccttcctc ggtgatcacg aggtggcgaa tggcgcgctc gctcatcaac
     2881 gcgttggcct cgaccgcgct cttcttgata tcgagggtga tg
//
LOCUS       DBNGTT010000089         2682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_402712, whole genome shotgun sequence.
ACCESSION   DBNGTT010000089 DBNGTT010000000
VERSION     DBNGTT010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_402712"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /gene="lpxB"
                     /locus_tag="AC16BC_02065"
     CDS             complement(<1..1030)
                     /gene="lpxB"
                     /locus_tag="AC16BC_02065"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR3240429.1"
                     /translation="MASLFIIAGEASGDGHGGKLAAALLARDPSLVLEGIGGAAMEKA
                     GVRLSHDIRHLGVVGVVEVLSQWRTIWRAYDGVRRRLREAPPAAIVLIDYPDFNLRIA
                     REASRLGIPVVYYVSPQIWAWRPGRIGAIKACVDLMLVLFQFEAALYERAGVPCRWVG
                     HPLLDDPSPDADHAAFVRRHRIERDQRVLALLPGSRAQEVGRLLPTMLEAAGRLRASG
                     ATDRVLIAAAPSVDARTIDDAVSRAGDAASAVTVVRGEAASVLRASRAALVASGTATL
                     QAAVVGTPLVMAYRVSPLSAWIGRRLITTPHQALVNVVAGRRVVPELVQDQVTAAGLA
                     AAVAPLLTD"
     gene            complement(1118..2071)
                     /locus_tag="AC16BC_02070"
     CDS             complement(1118..2071)
                     /locus_tag="AC16BC_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3240430.1"
                     /translation="MSERIKVGVIGVGYLGRHHARLYAELPGAELVGVADTNAARAQE
                     IAAASGARAFADDRELVPLVDAVSVVVPTSLHYAVTADCLAAGLDVLLEKPMTVTLDE
                     ADRLTALADSKQRLIQIGHLERFNGAVRAVSSRLSAPRFIESHRLGPFVGRGTDVHVV
                     LDLMIHDLDIILSLVRSPIADIRAVGVPVLTSNIDIANTRLEFADGCVANVTASRVSK
                     EPMRKLRIFQPDAYFSLDYQKQEVVMARRLEAPPAAGLPAIDVQTLVIEKEEPLKAQL
                     AAFLECVATRQAPLVSGREGREALRVALDVLKCIEGGRSPS"
     gene            complement(2249..>2682)
                     /locus_tag="AC16BC_02075"
     CDS             complement(2249..>2682)
                     /locus_tag="AC16BC_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HIR3240431.1"
                     /translation="YLPSLIAEPGVLTSNPSKEQWEDIRFGVEMVRAIGRIEIGQCVV
                     VKNRTVLAVEAIEGTDEAIRRGGRLAHGGAVVVKMAKPQQDLRFDIPAVGPATIASMR
                     EVGAAVLAVEAGKTLMLDKAELLAAACEAKIAVVGISPDDS"
BASE COUNT          431 a         1027 c          844 g          380 t
ORIGIN      
        1 catcggtcaa caagggcgcc accgcggccg ccaggcccgc ggccgtcacc tgatcctgca
       61 ccagctcggg caccacgcgc cgtcccgcca ccacgttgac cagcgcctgg tgcggggtgg
      121 tgatcaaccg gcggccgatc caggccgaga gcggggacac gcggtacgcc atcaccaacg
      181 gcgtgcccac cacggccgcc tgcagcgtgg cggtacccga cgcgaccaac gccgcccgcg
      241 acgcccgcag caccgacgcg gcttcgcccc gcaccacggt cacggccgag gccgcgtcgc
      301 ccgcgcgcga gaccgcgtcg tcgatcgtgc gcgcgtccac ggagggcgcc gcggcgatca
      361 acacccgatc cgtcgccccc gaggcgcgca gccgcccggc cgcctccagc atggtgggca
      421 gcagccgccc gacctcctgc gcgcggctgc ccggcagtaa cgccaacacc cgttggtcgc
      481 gctcgatccg atgccgccgc acgaacgccg cgtggtccgc gtctggcgac gggtcgtcca
      541 gcagcggatg cccgacccac cggcagggca cgcccgcgcg ttcgtacaac gcggcttcga
      601 attgaaacag gaccagcatc agatccacgc acgccttgat cgctccgatc cgccccggtc
      661 gccatgccca gatctgcggg ctgacgtaat acaccaccgg aatccccagg cgcgacgcct
      721 cgcgcgcgat ccgcaggttg aagtcggggt agtcgatcag cacgatcgcg gcgggcggcg
      781 cctcgcgcag gcggcggcgc acgccgtcat acgcgcgcca gatcgtccgc cattgcgaca
      841 acacctcgac cacgcccacc acgccgagat ggcggatgtc gtgggacagg cggaccccgg
      901 ccttttccat ggccgcgccg ccgatgcctt ccagcaccag cgacggatca cgggccagga
      961 gggccgccgc gagttttccg ccgtggccgt cccctgacgc ttcgccggcg atgatgaaca
     1021 gcgatgccat gcgtgaaacg atcctcttat ggaggggcga accttgtgat cgcccgcgtg
     1081 acggaatcgc agggccgggc gaatacacga ttcgccccta cgatggactc cgtcccccct
     1141 cgatgcattt gagcacgtcc agcgccaccc gcaatgcttc gcggccctcc cgcccggaaa
     1201 ccagcggcgc ctgccgggtg gccacgcact ccagaaacgc cgccagttgg gccttgagcg
     1261 gctcttcctt ttcgatgacc agcgtctgca cgtcgatcgc cggcaacccc gcggccggcg
     1321 gcgcctccaa gcgacgcgcc atcaccactt cctgtttctg atagtccagc gaaaagtacg
     1381 cgtcgggctg gaagatccgg agctttcgca tcggctcctt cgagacccgg ctggccgtca
     1441 cgttggccac gcacccgtcc gcgaactcca agcgggtatt ggcgatgtcg atattggacg
     1501 tgagcacggg cacgcccacc gcccggatgt ccgcgatcgg ggaccggacc aggctcagga
     1561 tgatgtccag atcgtggatc atcagatcca gcaccacgtg cacgtccgtg ccccgcccca
     1621 cgaacggccc caggcggtgc gactcgatga accgcggcgc ggacagccgc gaactcaccg
     1681 cgcgcaccgc gccgttgaac cgctcgaggt gtccgatctg gatcaaccgt tgcttggagt
     1741 cggcgagcgc cgtcaagcgg tcggcctcgt cgagcgtgac ggtcatgggc ttttccagca
     1801 acacgtccag ccccgcggcc agacagtccg ccgtgaccgc gtagtgcagg ctcgtgggca
     1861 ccaccacgct caccgcgtcc accaacggca ccagctcgcg gtcgtcggcg aacgcgcgcg
     1921 cgccggaggc ggccgcgatt tcctgggcgc gcgccgcgtt ggtgtcggcc accccgacga
     1981 gttcggcgcc gggcaactcc gcgtacaacc gggcgtggtg tcggccgagg tagcccacgc
     2041 cgatcacgcc gaccttgatc cgctcactca tggcgttcgt ccctccagcc cggatgattc
     2101 atgaacgttc gtcgcgaacg cgcgcagacg ccagcgagcc gggcgctcgg aaccgcgagg
     2161 gagggaggcg cactctctgc ggtacgtcga ccgactgagc ggcgagaccg cccggcgcag
     2221 cggcatctgc gcgggtgcag cagaatggtc atgagtcatc cgggctaatt cccaccaccg
     2281 cgatcttcgc ctcgcacgcc gcggccagga gttcggcctt gtccagcatc aaggtttttc
     2341 ccgcctccac ggccagcacc gcggcgccga cctcccgcat cgacgcgatc gtggccggcc
     2401 ccaccgccgg gatgtcgaac cgcagatcct gttgcggctt ggccatcttg accaccaccg
     2461 cgccgccgtg ggccagccgg ccgccgcgac ggatcgcctc gtcggtcccc tcgatcgcct
     2521 ccaccgccag caccgtgcgg ttcttcacca ccacgcattg tccgatttca atgcggccga
     2581 tggcgcggac catctccacg ccgaaccgga tgtcctccca ctgttccttg gagggattcg
     2641 aggtcaacac gcccggctcc gcgatcaaac tgggcaggta cc
//
LOCUS       DBNGTT010000090         4033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1168912, whole genome shotgun sequence.
ACCESSION   DBNGTT010000090 DBNGTT010000000
VERSION     DBNGTT010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1168912"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1318
                     /gene="ispG"
                     /locus_tag="AC16BC_02080"
                     /gene_synonym="gcpE"
     CDS             200..1318
                     /gene="ispG"
                     /locus_tag="AC16BC_02080"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3240432.1"
                     /translation="MNIARHKTRQIKVGNVAIGGDAPISVQSMTTTDTSQIDKTVAQI
                     AELERAGCEIVRVAVPDVPAAEALPEIRRQTTLPLIADVHFDYKLGLAALEAGVDCLR
                     INPGNIGDRDKVERVVKAAKERRTPIRIGVNAGSLERELLEKYGYPTPEAMVESALGH
                     IRILEDLDFSDIKVSLKASHVGLAVAAYRAFSAVSDYPLHLGITEAGTAFSGGIKSAV
                     GLGILLAEGIGDTIRVSLAADPVEEVKTGFEVLKALDLRRRGVNVVACPTCGRLEIDV
                     IRMANEVERRLAHLKEPLTVSILGCVVNGIGEGKEADIGIAGGRGVGLLFKHGEIVRK
                     VPETELMDVLLAEIDAMLAERGVSPPPALVDRDLPVVS"
     gene            1335..3053
                     /locus_tag="AC16BC_02085"
     CDS             1335..3053
                     /locus_tag="AC16BC_02085"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3240433.1"
                     /translation="MARYSNLLIPTLREDPGEAETVSHRLLLRAGMIRKVAAGIYNFL
                     PLGYRVLKRVERIVREEMDRAGAQEILMPFVQPADLWEETGRWGQYGKELARLKDRHD
                     RNFCLGPTHEEVVTDLVRREVKSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFLMKD
                     AYSFDRDDAGSEKSYEGMYRAYERIFERCGLTFRAVEADAGLIGGNFSHEFMVLAETG
                     EDAIAACDRCGYGANVERAEAKAPAAEPGNRSHVPPAEEVSTPRQHTVAEVAERLGVP
                     AARLIKTMLYQADDDVVAVLIRGDHEANEVKLKRYLKVDHVTLASDAAITRVTGGPKG
                     FSGPVGLNDVKIIADHAAAAVSDAVVGANKAGTHLAHVEPFRDFQINAITDLRNVVTG
                     DPCPRCADGHLTVSRGIEVGHVFRLGTKYTDKMKAVFLDQDGKEQTIIMGCYGIGVSR
                     IMAAAVEQNHDAKGIIWPAPLSPYAAHIIPLSDKPAVAEATARIEAELVKAGVEVLID
                     DRSERPGVKFNDADLLGISLHVVLGDKSLAQGEVEFKERRTGNVRKIRLDDAAGAIIA
                     WANASQ"
     gene            3089..3973
                     /locus_tag="AC16BC_02090"
     CDS             3089..3973
                     /locus_tag="AC16BC_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019272.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR3240434.1"
                     /translation="MSTVRSHCAAAVHYNRIYVFGGGGHAFAALNSAECYDPKADRWS
                     PIAAMPTARSGIVAVPFQNKIYVMGGGFKKPDGTFQFLTVVEIYDPEKDSWTKGPDLL
                     RRHDAPAATVMNGRIYLVGGHLPDTPGGPLTDPAFNFSEVFDVFQNRWLELTPMPTPR
                     FSLAAVPWEGKLLAMGGGAFRDGAFRNFDVIEAYNPTKGAWEPSDIGPLPWPTAGLAA
                     LVVDNAIHAFGGNDGHGIQPKAARYTREAGWAMLPPMPEPRAAAAAVPLGGAIYLIGG
                     RDAGGKTPTETILLWRPD"
BASE COUNT          736 a         1278 c         1339 g          680 t
ORIGIN      
        1 gtgttcttct tgatcgaagc cgtgatcggg catccgctga gccggcgggg gttggagatt
       61 gcgcaaaccg tgggcatggt cttgctcatc acgctggcat tggttgtttc ttataacgat
      121 attatgagaa tcatcggtta actcctgttc ccttatcccc ctttgaaaaa ggggggaagg
      181 gggaatttgc ttctgacaga tgaacatcgc gagacacaaa actagacaga tcaaagtcgg
      241 taacgtggcc atcggcggcg acgcgccgat ttcggtgcag tccatgacca ccaccgacac
      301 gtcgcagatc gacaagaccg tggcgcagat cgcggagctc gaacgcgcgg ggtgcgagat
      361 cgtgcgcgtg gcggtgccgg acgtgcccgc ggccgaggcg ttgccggaga ttcgccggca
      421 gaccacgctg ccgctgatcg cggacgtgca cttcgactac aaactcgggc tcgccgcgct
      481 ggaggcgggc gtggattgtc tgcggatcaa ccccggcaac atcggggatc gcgacaaggt
      541 cgagcgcgtg gtcaaggcgg ccaaggagcg ccgtaccccg atccgcatcg gcgtgaacgc
      601 gggttcgctg gagcgcgaat tgctcgaaaa gtacgggtac cccacgccgg aggccatggt
      661 ggaatccgcg ctcggccata tccgcattct ggaagacctg gacttttccg acatcaaagt
      721 gtcgctcaag gcctcgcacg tgggcctggc cgtggccgcg taccgcgcgt tctccgcggt
      781 cagcgactac ccgttgcatc tgggcatcac cgaggccggg accgcgttct cgggcggcat
      841 caagtccgcg gtgggcctgg gtatccttct ggccgagggc atcggcgata cgatccgggt
      901 ctcgctcgcg gccgatccgg tcgaagaggt caagaccgga ttcgaagtgc tcaaggcgct
      961 cgacctgcgc cgtcgtggcg tcaacgtggt ggcctgtccc acttgcgggc ggttggagat
     1021 cgacgtgatc cgcatggcca atgaagtcga gcggcgcctc gcgcacctca aggagccgtt
     1081 gaccgtctcg attctcggct gcgtggtcaa cggcatcggc gagggcaagg aagccgacat
     1141 cggcatcgcg ggcggtcgcg gcgtgggcct gctcttcaag cacggcgaga tcgtgcgcaa
     1201 ggtgcccgag accgaattga tggacgtctt gctcgcggag atcgacgcga tgctggcgga
     1261 gcgtggcgtg tcccccccgc cggcgttggt tgaccgcgac cttcctgtcg tttcgtagac
     1321 cccggcatcg gatcatggct cggtattcca atttattgat tcccacgctt cgggaagatc
     1381 ccggcgaggc cgaaaccgtc agtcaccggc tcctgctgcg cgcgggcatg atccgcaaag
     1441 tcgcggccgg gatctacaac tttctgccgc tcggctatcg cgtgctcaag cgcgttgagc
     1501 gcatcgtgcg cgaggagatg gatcgcgcgg gtgcgcaaga gatcctgatg ccgttcgtgc
     1561 agcccgcgga cctgtgggag gagaccggcc ggtgggggca gtacggcaag gaactcgcca
     1621 ggctcaagga ccgccacgac cgcaactttt gtctgggtcc cacgcacgaa gaggtggtca
     1681 cggacctggt gcgtcgcgag gtcaagtcct accgccagtt gccggtcacg ctgtatcaga
     1741 tccagtccaa gttccgcgac gagatccgcc cgcgcttcgg cttgatgcgc ggccgcgagt
     1801 tcttgatgaa ggacgcgtac agcttcgacc gggacgacgc ggggagcgag aagagctacg
     1861 aaggcatgta ccgcgcgtac gaacggattt tcgagcgctg cggcctgacg tttcgggccg
     1921 tggaggcgga tgcgggactg atcgggggaa acttttcgca cgaattcatg gtgctggccg
     1981 agacgggcga ggacgcgatc gccgcatgcg atcgctgcgg ctacggcgcc aacgtggagc
     2041 gcgcggaggc caaggctcct gcggctgagc ccggcaatcg atcccatgtc ccgcccgccg
     2101 aggaagtgag cacgccgcgt caacacaccg tggcggaggt ggcggagcgt ctcggcgtgc
     2161 ccgccgcgcg actgatcaag acgatgttgt atcaggcgga tgacgacgtc gtggcggtgt
     2221 tgattcgcgg cgaccacgag gccaacgagg tcaaactcaa acggtatttg aaagtcgatc
     2281 acgtgacgtt ggcgtcggac gccgccatca cgcgcgtcac gggcgggccc aagggctttt
     2341 cgggtccggt cggactcaac gacgtgaaga tcatcgcgga ccatgccgcg gccgcggtga
     2401 gcgacgcggt ggtgggcgcg aacaaggccg gcacgcacct cgcgcacgtg gagccgttcc
     2461 gggatttcca gatcaacgcg atcacggact tgcgcaacgt ggtcacgggc gacccgtgcc
     2521 cgcgctgcgc cgacggccat ttgaccgtct cgcgcgggat cgaagtgggc cacgtgttcc
     2581 gcctgggcac caagtacacg gacaagatga aggccgtgtt cctggatcag gacggcaagg
     2641 aacagacgat catcatgggc tgttacggca tcggcgtgag ccgcatcatg gcagcggccg
     2701 tggagcagaa ccacgacgcc aaaggcatca tctggcccgc gccgctctcg ccctatgccg
     2761 cgcacatcat tccgctgagc gacaaacccg cggtggcgga ggccacggcg cgtatcgaag
     2821 ccgagctggt caaagccggc gtcgaggtgt tgatcgacga tcgctccgag cggcccggcg
     2881 tgaaattcaa cgacgcggat ctgctcggca tctcgctgca cgtggtgctc ggcgacaaat
     2941 cgctcgccca gggcgaagtc gagttcaaag agcgccgcac cggcaacgtg cgcaaaatca
     3001 gactcgacga cgcggccggc gccatcatcg cctgggccaa cgcttcgcaa tgaccgcgcg
     3061 cgcgggcacc tggacgctcg cgcgacccat gtccacggtc cgctcgcact gcgccgcggc
     3121 cgttcattac aatcgcatct acgtgttcgg gggcggcggg cacgccttcg ccgcgctcaa
     3181 cagcgcggaa tgctacgacc ccaaggctga tcgctggtcg ccgatcgccg ccatgcccac
     3241 cgcgcgatcc ggcatcgtgg ccgtcccgtt tcagaacaag atctatgtga tgggcggggg
     3301 cttcaagaaa cccgacggca cgttccagtt cctcaccgtg gtcgagatct acgaccccga
     3361 gaaagactcc tggaccaaag gcccggactt gctccgccgc cacgacgcgc ccgccgccac
     3421 cgtgatgaac ggccgcatct acctggttgg cggccacttg cccgacactc cgggcgggcc
     3481 attgaccgac cccgcgttca atttctccga agtgttcgac gtgttccaaa accgctggct
     3541 ggaactcacc cccatgccca ccccgcggtt ctccctcgcc gcggttccat gggaaggcaa
     3601 gctgctagcc atgggcggcg gcgcgtttcg cgatggcgcg ttccgcaact tcgacgtgat
     3661 cgaagcgtac aacccaacga agggcgcctg ggagccatcc gacatcggtc cattaccctg
     3721 gcccacggca ggcctagccg cgctcgtcgt cgataacgca atccacgcgt tcggcggcaa
     3781 cgacggccac ggcatccagc ccaaagccgc ccgctacacg cgcgaggctg gctgggccat
     3841 gctcccccca atgcccgaac cgcgtgctgc ggccgcggcg gtcccgttgg gtggagcgat
     3901 ctatctgatc ggggggcgtg atgcgggtgg gaagacgccg acagaaacga tactgctctg
     3961 gcgaccggac taaaggcatt cgatgttgtg cgatggcgtc aacaacatga gagcggattg
     4021 acaccccagt caa
//
LOCUS       DBNGTT010000091         1683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2829524, whole genome shotgun sequence.
ACCESSION   DBNGTT010000091 DBNGTT010000000
VERSION     DBNGTT010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2829524"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="AC16BC_02095"
     CDS             complement(<1..212)
                     /locus_tag="AC16BC_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HIR3240435.1"
                     /translation="MKTWLEGPGFLSPYGTMGADLSFVLAVGFTVMFLVGWRKAKQHK
                     GQAHHVVTLWAMLAMIAYFVAYYVNRG"
     gene            complement(364..1443)
                     /locus_tag="AC16BC_02100"
     CDS             complement(364..1443)
                     /locus_tag="AC16BC_02100"
                     /inference="COORDINATES: protein motif:HMM:NF037020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3240436.1"
                     /translation="MLPASWRAHPAARYLPATLAILSIAAAPFGLIALLGESSTSSGA
                     PELVGLQGWIGSRPLTLAGLRGSVVLVDFWTYSCINCLRTIPYLNAWYDRYRDDGLVV
                     IGIHSPEFAFERDPARVESEVRRLGVSYPVAIDSEKRTWAAYANQYWPHKYLIDRGGH
                     IRHTQIGEGGYELMESHIRALLAEQGHVVSAESASVSPEAVDAKAIATPEIYFGSYFG
                     QFLGNPLGLRGMRGEAEYREPDALEPNLFYLSGRWTIGEEAVTSVGRDAHRVSLHYTA
                     KAVNVVAGAPGRRVLVEVLRNGRPVDEAEWGTDVRRDERGRTVVEVSDHRLYRLINDR
                     AGYGTHTITLGIDEPGLDVYTLTFG"
BASE COUNT          308 a          589 c          498 g          288 t
ORIGIN      
        1 ccccggttta cgtagtaggc cacgaagtag gcgatcatcg ccagcatggc ccaaagcgtc
       61 accacgtggt gcgcctggcc cttgtgctgt ttcgccttgc gccagcccac cagaaacatg
      121 acggtaaaac cgacggccaa cacaaaactc agatccgcgc ccatggtccc gtacgggctc
      181 aggaacccgg gcccctccag ccatgttttc atccgcccct cctctcctcg cttgcgaagc
      241 gcaacgcagg cgcgcattcc gcgccgagaa cctgcaccgt gccaacggca cggaatgcga
      301 atcgccactt cctaccaggt ggcgaagcac tgcgagatgt ctttgcccct cgggttcttc
      361 tgtctatccg aaggtcaagg tgtacacgtc gagtcccggc tcatcgattc ccagcgtgat
      421 cgtgtgtgtt ccgtaccccg cgcggtcatt gatgagtcga tacagccgat ggtcgctcac
      481 ttccaccacc gtgcgcccgc gttcgtcgcg tcgcacgtcc gtgccccact cggcctcgtc
      541 caccggtcgc ccattgcgca ggacttcgac cagaacccgc cgtcccggag cgcccgccac
      601 cacgttgacc gccttggccg tgtagtgcaa cgacacccgg tgcgcgtccc ggcccacgga
      661 ggtcaccgct tcctcgccga tcgtccaccg accagacaga taaaagagat tcggttccag
      721 cgcgtccggt tcccggtact cggcctcccc gcgcatcccg cgcagcccga gcggattgcc
      781 cagaaactgc ccgaaatacg atccgaagta gatctccggc gtggcaatgg ccttggcgtc
      841 cacggcctcg ggcgacaccg acgcgctctc ggccgacacc acgtgcccct gctccgccaa
      901 caacgcgcga atgtgcgatt ccatcagctc atagccgccc tcgccgatct gggtatggcg
      961 gatgtgcccg ccgcggtcga tcagatattt gtgcggccag tactgattcg cgtacgcggc
     1021 ccacgtgcgc ttctcgctgt cgatcgccac cgggtacgag acgcccagcc ggcggacttc
     1081 ggactcgacg cgggccggat cgcgctcgaa cgcgaactcc gggctgtgaa tcccgatcac
     1141 gaccaaaccg tcgtcgcgat accggtcgta ccacgcgttg agatacggga tggtccgcag
     1201 acaattgata caactatacg tccagaaatc gacgagcacg acgctgccgc gcaatccggc
     1261 cagcgtcagg ggtcgcgagc cgatccagcc ctggagcccg accaactcgg gcgcgccgct
     1321 cgacgtcgaa gactcgccca acaacgcgat caagccaaac ggcgcggccg cgatactcag
     1381 aatcgccaga gtcgcgggga ggtatcgagc ggctgggtgg gcgcgccacg acgcagggag
     1441 catcgtcagc agggaatcga agagttcgct tgcgcgggtt gcgggggttg ccccggctgt
     1501 ccagcctgcg gttcatattg gaacttccaa tcgcggtacg tgcccgcaga gcgaaactgc
     1561 tcgtatgcgg ccgggaagtt atcgtgtttg agcgggatcg cgtcactggg actgacgacc
     1621 cccctgatcc tatccctgca gggaatcgcg tggaacggcc cgcgggtgat cgggtccagg
     1681 tag
//
LOCUS       DBNGTT010000092         4071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2830163, whole genome shotgun sequence.
ACCESSION   DBNGTT010000092 DBNGTT010000000
VERSION     DBNGTT010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2830163"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="AC16BC_02105"
     CDS             <1..296
                     /locus_tag="AC16BC_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HIR3240437.1"
                     /translation="TEEARKDLNRNLFRGVNELELSVRAANCLKNANIKTIADLVQKT
                     EHEMLKTKNFGRKSLNEIKDLLHEMGLSFGMNIKDLLPAEEGVEAEAKPEGVA"
     gene            298..744
                     /gene="rplQ"
                     /locus_tag="AC16BC_02110"
     CDS             298..744
                     /gene="rplQ"
                     /locus_tag="AC16BC_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR3240438.1"
                     /translation="MRHRKAGRQLGRNSGHRRALFRGLVTSLLEHERIETTVPKAKEL
                     RSFAEKMITLGKKGDLAARRRALAFIRKESVVRSLFSDIAPRFANRAGGYTRVVHSRR
                     RLGDGAPMAIIELTELKPKVTTAGPEKKAKGAKKEKPEAEKAKAAG"
     gene            complement(1303..1845)
                     /locus_tag="AC16BC_02115"
     CDS             complement(1303..1845)
                     /locus_tag="AC16BC_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3240439.1"
                     /translation="MGKLHDHIDDDLAAWIHRQRVFFVATAPLSPDGHVNCSPKGGDA
                     FRVLGPHTVAYQDLTGSGVETIAHLRENGRIVIMFCAFEGPPSIVRLHGRGDALTPDH
                     PKFASLRKHFPDHAGTRAIIRVHVTRVSDSCGFGVPLFDHRCDRDALDTWAESKGPEK
                     LQEYRRAKNARSVDGLAGLE"
     gene            2030..2653
                     /locus_tag="AC16BC_02120"
     CDS             2030..2653
                     /locus_tag="AC16BC_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR3240440.1"
                     /translation="MDALTFLQDPDALDRFVLVFLPIFVAIDVIGVLPTYYSLTHALP
                     AKDKPRIARQSVVTAFSVTVMFILLGKATFSVLGVRVEDFMIAGGVLLLVFAIWDLLR
                     DGTRASSPKSQQTLGVVPLGTPIIAGPATLTTSLMLMGTYGVGLVALSLGLNLLLAWV
                     VVRYADRVIGLLGVNGATAIAKVFYLLLAAIAVSMIRRGVMGMLGVA"
     gene            2655..4031
                     /locus_tag="AC16BC_02125"
     CDS             2655..4031
                     /locus_tag="AC16BC_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR3240441.1"
                     /translation="MEPVATHQGRIGAYPLTPSQILVLWFAGAISIGTFALSLPVASA
                     PGHSIRFVDALFMATSAICVTGLAVKDLATDFSTQGQIMILVLVQLGGLGYMVLSTVI
                     IVLLGKKVGIKQRLVMQEALNVLSVEGLIRLLKGILWMTVVMEGLGAVLLATRFSFDF
                     PVPEAIYLGVFHSVTAFNNAGFSLFPTSLVGYQHDVLVNVVVTVLIISGGIGFIVYRD
                     LIRRIKRETYRLTVHTWMVLSLTGALILGGTLVFWALEAGNPKTIGTLPLADQFMASY
                     FHSVSSRTAGFNTVDLGAGTPATLYILVLLMFVGASPGGTGGGIKTSTFGTMLVGIWA
                     GIRGRSDVTAFHRRIPQETVIRAFSLALVAFALTTGMTLVLEVSERQDFLRTLFEVAS
                     AFGTVGLSTGDGGVLSYSALFSDFGNLMIMLTMFIGRIGPLTVSVALVRLEETRRFRY
                     PPERVLIG"
BASE COUNT          700 a         1171 c         1322 g          878 t
ORIGIN      
        1 tcacggaaga ggcgcgcaag gacctcaacc gcaatttgtt ccgcggggtc aacgagctgg
       61 agttgtcggt gcgcgcggcc aactgcctga agaacgcgaa catcaagacc atcgcggatc
      121 tggtgcagaa gaccgagcac gaaatgctca agaccaagaa cttcggccgc aagtcgctca
      181 acgagatcaa ggacctgctg cacgagatgg ggctctcgtt cgggatgaac atcaaggatc
      241 tgctcccggc ggaagagggt gtggaagccg aagccaagcc cgagggggtg gcctgacatg
      301 agacaccgta aagccgggcg ccagttgggg cgcaacagcg gccaccggcg cgccctgttc
      361 cgggggttgg tgacctcgct cctcgaacac gagcgaatcg agacgaccgt gccgaaagcc
      421 aaagagctcc ggtcctttgc cgagaaaatg atcacgctcg gcaagaaggg cgacttggcc
      481 gcgcgccgtc gcgcgctggc gttcatccgg aaggaatcgg tggtgcggtc gctcttctcg
      541 gacatcgcgc cgcggttcgc caaccgcgcg ggcggctaca cccgcgtggt gcactcccgc
      601 cgccgcctgg gcgacggcgc cccgatggcg atcatcgaac tgaccgaact caaacccaaa
      661 gtcaccaccg ccgggccgga gaaaaaggcc aagggcgcga agaaggaaaa gcccgaagcg
      721 gaaaaggcca aagctgcggg gtgaggtgat ccgacgatca gaaggtcaac gaacggcgca
      781 gcctgaaggt tgcgccgttc gtgttttgaa cgaggaccct tggacttttc agatgtcaaa
      841 gtcaagacgt gaccccggcg ggtgtgccac tcagagacag atgcctacta aaagattctt
      901 ctttgtagtt ggttatattt tgatcgtctt tgtaatcgtg gtcctgcttg cttcgaagtt
      961 cggctatatc aaacctctat atgattatat ttctcgtatt atgagcgatc gagcttcgtt
     1021 tgaaagtagt cgcctcatag gatttgttct atggttctcc gtaacgattc tgttcgtagt
     1081 cttaggcgac ggaatggcca aacggaaaag gcggcgccga catgtctgga ttatcttatg
     1141 tgcattattc aatttctggg cgtggctcta tctctattat ttgccaactg cgtcagagaa
     1201 cacttcaact cgttagcaat acaatcgcac aacatctctg cggtagccct gattctctgg
     1261 acggattgcc gagcacacgc ctcgaccctg gcagggatgc ttctattcca atccggctaa
     1321 tccgtccacg ctcctggcat tcttggcgcg gcgatattcc tgcaatttct ccggtccctt
     1381 agattctgcc cacgtgtcca gcgcatcacg atcgcaccgg tgatcgaaca gcggcacgcc
     1441 gaacccgcat gaatccgaca cgcgtgtgac gtgaacgcgg atgatggctc gcgttccggc
     1501 gtggtctggg aagtgttttc gcagcgaggc gaacttcgga tggtccgggg tgagcgcatc
     1561 gccgcggccg tggagccgga cgatggaggg tgggccttcg aaggcgcaga acatgatgac
     1621 gatgcggccg ttctcccgga ggtgcgcgat ggtttccacg ccgctgccgg tcagatcctg
     1681 atacgccacg gtgtgagggc ccagcacgcg gaacgcgtcg ccgcctttgg gcgagcagtt
     1741 cacgtggccg tctggcgaga gcggcgccgt ggccacgaag aagacgcgct ggcggtggat
     1801 ccaggccgcg aggtcgtcgt cgatgtggtc gtggagtttg cccatggcgt gtgaggggtt
     1861 tctctcacgt ataagccgtt tcaacccggg agtcaatcgc ccgcccgcgt caagtttgcg
     1921 gccggacatt ccttgacagc atggcccggc gggcctatga tgcccgccgt atcgcgaggg
     1981 gtcgcgcatc ggccggacag ggaagtgacc ggccgcggct cacgctcaca tggacgcgct
     2041 gacgttcctc caagatcccg acgcgctcga tcgcttcgtc ctggtgttcc tgccgatctt
     2101 cgtggcgatc gacgtcatcg gggtgttgcc cacgtattac tcgctcacgc acgcgctccc
     2161 ggccaaggac aagccgcgca tcgcgcggca gtccgtggtc accgcgtttt ccgtgaccgt
     2221 gatgttcatc ctgctgggca aggccacgtt ttccgtgctt ggggtgcggg tcgaggactt
     2281 catgattgcg ggcggcgtgc tgctgctggt ctttgcgatc tgggacctgc tgcgggacgg
     2341 cacccgcgcg tcctcgccca agtcgcagca aaccctgggc gtggtgccgc tgggcacgcc
     2401 cattatcgcg ggccccgcca cgctcaccac gtcgctgatg ctgatgggca cgtacggggt
     2461 cgggctggtg gcgctctcgt tggggctcaa cctgctcttg gcgtgggtcg tggtgcggta
     2521 cgcggacagg gtgatcgggt tgctgggcgt caacggcgcg accgccatcg ccaaggtgtt
     2581 ctatctgctg ctggccgcga tcgcggtgag tatgattcga cgcggcgtga tgggtatgct
     2641 gggagtggcc tgagatggag ccggtcgcca cccaccaagg ccgcatcggg gcgtatccgc
     2701 tgacgccgtc gcagattctt gtcttgtggt tcgccggggc gatctcaatc gggacgttcg
     2761 ctctctcgct tccggtggcg tcggcgccgg gtcattcgat ccggttcgta gacgcgttgt
     2821 tcatggccac gtccgccatc tgcgtcacgg gtcttgcggt caaggatctt gccaccgact
     2881 tcagcaccca gggccagatc atgattttgg tcctggtgca gttgggcggg ttgggataca
     2941 tggtgttgtc cacggtcatc atcgtccttt tgggcaagaa ggtcgggatc aaacagcggc
     3001 tggtcatgca ggaggcgctc aacgtcttat ccgtggaggg gctgatcagg cttctcaagg
     3061 gcattttgtg gatgacggtg gtgatggaag gcctcggcgc ggtgctgctc gcgacccggt
     3121 tctcctttga tttcccggtt ccggaggcga tctacctcgg ggtgtttcat tccgtgaccg
     3181 cgtttaacaa cgcggggttc tccttgtttc ccaccagtct ggtcgggtat cagcacgatg
     3241 tgttggtcaa tgtcgtggtg acggtcctta tcatctcagg cggcatcggg tttatcgtgt
     3301 accgggattt gattcgccgg atcaaacgcg agacgtaccg tttgaccgtg cacacgtgga
     3361 tggtgttgag cctgaccgga gcgctgattc ttggcggcac gctggtattc tgggcacttg
     3421 aagccggcaa ccccaagacc atcgggaccc tgccgttggc ggaccagttc atggcgtcct
     3481 atttccattc ggtgtcctcg cgcaccgcgg gattcaatac ggttgacctg ggagccggca
     3541 cgcccgccac cctgtatatt ttggtcctgc tcatgttcgt gggcgcgtcg ccgggcggca
     3601 ccggcggcgg gatcaagacc tcgacgttcg gaaccatgtt ggtggggatc tgggccggca
     3661 tccgcgggcg aagcgacgtg accgcgttcc atcgacggat tccgcaggag accgtgatcc
     3721 gggcgttctc gctggcgttg gtggcgttcg ccctgaccac ggggatgacg cttgttcttg
     3781 aagtcagcga gcggcaggac ttcttgcgta ccttattcga ggtcgcgtcg gcgttcggaa
     3841 ccgtggggct ctccaccgga gatggcggcg tgctgagcta ttccgcgctg ttttcggatt
     3901 tcggcaatct catgatcatg ctgaccatgt tcatcggccg gatcggcccg ctgaccgtca
     3961 gcgtggcgtt ggtgcggttg gaagaaacgc ggcgctttcg gtatcctcca gagcgcgttc
     4021 tcatcggcta ggggcacacc atggctcgcc agtttgcggt gatcggtctg g
//
LOCUS       DBNGTT010000093         6437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_20245, whole genome shotgun sequence.
ACCESSION   DBNGTT010000093 DBNGTT010000000
VERSION     DBNGTT010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_20245"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="AC16BC_02130"
     CDS             complement(<1..511)
                     /locus_tag="AC16BC_02130"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3240442.1"
                     /translation="MDDHASSETPDARPPIASDLRETLLGARPVYEGRYLRVEERTVR
                     LPDGREGRREIVMPPNAVGVLPVTDAGIVYLVRQYRTAIERAILEIPAGILEPGEDPA
                     QTARRECAEEIGLRPDRLDPLFGYYHSVGFSTGRIEVFLGRDLRPAPHAKPDGTEFLE
                     VVTMPFDDLF"
     gene            620..1180
                     /locus_tag="AC16BC_02135"
     CDS             620..1180
                     /locus_tag="AC16BC_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HIR3240443.1"
                     /translation="MGDERVMKTWERVFEGALWRSRFIVLLAVVASLASAVILFLIAT
                     VDVASLGVKAVGYAGGGPHSAEEYDAFHSMVVGHVISAVDDYLLATVLLIFSLGLYEL
                     FISKIDVAQEDARHSSKILLIQTLDDLKDRLAKVVLMILIVTFFKNVIHTSFSEPLGI
                     LYLGVGILFVALALYFTARSGHGSEK"
     gene            1177..2100
                     /locus_tag="AC16BC_02140"
     CDS             1177..2100
                     /locus_tag="AC16BC_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR3240444.1"
                     /translation="MSWARLPDLSVARSHPAVAVHDGRLYAIGGGGPGFASLDSVEVF
                     DPATERWRTARPMPTKRSGAMAVSVGGRIAVIGGGFKKPDGKFRFLRTVELYDHATDQ
                     WSQGPDMFEPHDYPACAVLDGWIYIATGHHPGATEGGPQTDPGFSFTERWRPGEDAWE
                     ALPPAPTPRFAAAAVALGGRVAVAGGVAFTPDGFTEYDRVEMYDPARATWERAGWSLP
                     WTAAAHSLTIVGRRLVLCGGFSGETGIGAAASICDLDASDRPGAWRPLPAMPEPRAAM
                     GAAVLDGRLVLVGGWAADRSVMAGVRAWRDE"
     gene            2093..2734
                     /locus_tag="AC16BC_02145"
     CDS             2093..2734
                     /locus_tag="AC16BC_02145"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3240445.1"
                     /translation="MSDPAIPACVVGIVIFHGVEEIDFAAPYAVFSALRRLQIESGAS
                     DLPETHVLTISRTPGIIHGANGLRWLPDFDLSMCPDLSVLVVPGCGEIGEPTVPEMEE
                     GGLWDFVHEQGTRARCVGGVGTGAFVLAKAGVLAGKRATTRARFRADLARFAGVTVAD
                     GPLVEDGAVWTAASGAWGVGLGLAVIARLYGRAAANRTAAELDAAWDESLIAP"
     gene            2770..4206
                     /gene="mpl"
                     /locus_tag="AC16BC_02150"
     CDS             2770..4206
                     /gene="mpl"
                     /locus_tag="AC16BC_02150"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR3240446.1"
                     /translation="MPAIKHVHLIAICGTGMGSLAGMLKASGYHVSGSDQNVYPPMST
                     WLAEQGIVPYSGFAPEHLDGADLVIVGNAVSKANPEVVAMLERGLPYRSFPQALAEFF
                     IRDRHSMVVAGTHGKTTTTALLAWVLTSAGRDPGAMIGGWAKNFDGNFRVGGGGVVAV
                     EGDEYDTAFFDKGPKFLHYRPRTAILTSIEFDHADIYRDLAHVVSAFERFVELIPRDG
                     LLVAAAGEETVARVAGRAASRVETYGFAAGSDWSAEGLSAREGGVSFDVRYAGARFGR
                     FDLPLIGRHNVLNALAVIAAAHGAGLDAREIAEGFRTFRGIKRRQDVVGVANDVVVMD
                     DFAHHPTAIRETLAAVKMGYPSRQLWAIFEPRSATSRRAVFQTQFAEAFDLADRVVIA
                     EPFASEKLPVEQRLDAKRLVDDLGRRGVHAAHLANADDIVRTVAPQLAPGDVICVMSS
                     GGFDGIHAKLLAALRAAEVRSLRPPKSA"
     gene            4203..5123
                     /locus_tag="AC16BC_02155"
     CDS             4203..5123
                     /locus_tag="AC16BC_02155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HIR3240447.1"
                     /translation="MTPLRKPPALRKGDLIGVVAPAGAVKTEDLNAGVKRLEELGYRV
                     LVGRSVYRAARYLAGRDAERAGDLMEMFANAEVRAIVAARGGYGTSRVIPLLDPRAIA
                     RSPKIVVGSSDVTLLLHFLQTACAFVTFHGPMVSPNFGKYSSPATNDSFVRVLGATAP
                     PGRFDVRGVTTLKGGSAEGVLTGGCLSLVCQTIGTPYEIQTDGAIVFLEDINEPPYRI
                     DRMLTYLKQLGKFDRARAVIFGLMPDCHPAPQESYRLHDVIRDVLGDLPCPILLDFPS
                     GHGGTNVTLPFGVRAAVDGATLSILEAPVS"
     gene            complement(5262..>6437)
                     /locus_tag="AC16BC_02160"
     CDS             complement(5262..>6437)
                     /locus_tag="AC16BC_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240448.1"
                     /translation="TYTHTGLDPGTPYYYVVTAVVGTDGESAASAEVQAIPAQTTGGV
                     TATASQGQVTITWPAGDTAAISVARSEVTYVSPFPTFPLTIGDLENGRRHAIWIRPLF
                     AGGLGPASPTMYAVPKASADGVPKTVTLSANRGVNTLSWDEIIGAAAYDVTWFAQDVD
                     GSPIAGTRTVSAPPFRHVGLRMCSVLGDACPTYTYRVRVAQSTGIAAEVAAQSADLRP
                     TPPIITNDDFVIITGVKPVGSRVEIDGVVTVPFDYDTGWTATLPLAGAADRLFTFRMV
                     AIDADGLVSTETTYQVTRDTAPPAPPQSVTLTACDPATDSPRGITLSGTKDAESAVFR
                     VMEPTAADQAIVGSTGQTTWSGVIELDDQPAAISLVAKDAAGNVSSPAISIALSACP"
BASE COUNT          938 a         2123 c         2340 g         1036 t
ORIGIN      
        1 cgaacaagtc gtcgaacggc atggtcacga cttcgagaaa ttcggtgccg tccggtttgg
       61 cgtggggcgc gggccgcagg tcgcgcccga gaaagacctc gatgcggccg gtggaaaacc
      121 cgaccgaatg gtagtagccg aacagcgggt ccagacggtc cgggcgcagc ccaatttcct
      181 cggcgcactc gcgccgggcg gtctgcgcgg ggtcttctcc gggttcgaga atgcccgcgg
      241 gaatctccag gatcgcgcgt tcgatcgcgg tgcggtattg ccggacgagg tacacgatgc
      301 cggcgtcggt gaccggcaac acgcccaccg cgttcggggg catgacgatc tcgcggcgcc
      361 cctcgcgacc gtcggggagc cgcacggtgc gttcctccac acgcagatag cggccctcgt
      421 acaccgggcg cgcgccgagc agcgtctcgc gaagatcgga cgcaatgggc ggccgggcat
      481 ccggcgtctc ggatgaggcg tggtcgtcca tgacgcgggt gatcgtacca cacgcgcggg
      541 cgcgccgcgc ggccgatcga caaccggact ccgctcccgt ataatgccgg tctcgcggtc
      601 gggagcggga tcacgaacca tgggcgacga acgggtcatg aagacgtggg aacgggtgtt
      661 cgagggcgcg ttgtggcgca gccggttcat cgtgctgctg gccgtggtcg cgagtctcgc
      721 gtccgcggtg atcctgttct tgatcgccac cgtggacgtc gcgagcctcg gggtcaaggc
      781 ggtcggctac gccggcggcg gcccgcactc cgccgaggaa tacgacgcct tccacagcat
      841 ggtggtggga cacgtgatca gcgcggtgga cgactacttg ctcgccaccg tgctgctgat
      901 cttttcgctc gggctctacg agctctttat cagcaagatc gacgtggccc aggaggacgc
      961 ccggcacagt tccaagatcc tgttgatcca gacgctcgac gatctcaagg accgtttggc
     1021 caaggtcgtc ctgatgatcc tgatcgtgac cttcttcaag aacgtgatcc atacgagctt
     1081 cagcgagccg ctcgggatcc tgtacctggg ggtcggcatc ttgttcgtgg cgctggcgtt
     1141 atacttcacg gcccggtccg ggcacgggag cgagaagtga gttgggcgcg cctccccgac
     1201 ctgtccgtgg cccggtccca tcccgcggtc gcggtccatg acggccgcct ctacgccatc
     1261 ggcggcgggg gacccggctt cgcgagcctt gatagcgtcg aagtcttcga tcccgcgacc
     1321 gaacggtggc ggaccgcgcg ccccatgccg accaagcggt cgggcgccat ggccgtgtcc
     1381 gtcggcgggc ggatcgcggt gatcggcggc gggttcaaga aacccgacgg caagttccgc
     1441 tttctgcgga ccgtggaact ctacgatcac gcgacggacc agtggtctca gggcccggac
     1501 atgttcgagc cgcacgacta tcccgcgtgc gccgtgttgg acgggtggat ctatatcgcg
     1561 accggtcatc accccggggc gaccgagggc gggccgcaaa ccgatccggg attttcgttc
     1621 accgaacgct ggcgtcccgg cgaggacgcg tgggaggcgt tgcccccggc gcccaccccg
     1681 cggttcgccg ccgcggcggt cgcgctcggc gggcgggtgg cggtggcggg cggcgtcgcg
     1741 ttcaccccgg acgggtttac cgagtacgac cgcgtcgaga tgtacgaccc ggcccgcgcg
     1801 acgtgggagc gcgccggatg gtcgctcccg tggaccgcgg cggcgcacag cctcacgatc
     1861 gtcggtcgac gcctcgtgct ctgcggaggg ttctccgggg agacggggat cggcgcggcc
     1921 gcgtcgatct gcgacctgga cgcctcggac cgtcccgggg cgtggcgtcc gctgccggcg
     1981 atgcccgagc cccgcgccgc gatgggcgcg gccgtgctcg acggacggct cgtcctggtg
     2041 ggcgggtggg cggccgaccg gtcggtcatg gccggcgtac gcgcgtggcg cgatgagtga
     2101 tccggcgatt ccggcctgcg tggtcgggat cgtgattttt cacggcgtgg aggaaatcga
     2161 cttcgccgcg ccctacgcgg tgttctccgc gctccggcgt ctccagatcg agtccggcgc
     2221 ttccgacctg ccggagaccc acgtcctgac gatcagccgc acgcccggca tcatccacgg
     2281 cgccaacggg ctgcgctggc tcccggactt cgacttgtcc atgtgcccgg acctctcggt
     2341 gctggtcgta cccggttgcg gggaaatcgg cgagcccacg gttccggaaa tggaagaggg
     2401 cgggctctgg gactttgtcc acgagcaggg gacgcgggcc cggtgtgtgg ggggcgtggg
     2461 gaccggcgcg ttcgtgctcg ccaaggccgg cgtattggcc gggaaacgcg cgaccacccg
     2521 cgcgaggttt cgcgctgact tggcgcgttt cgcgggcgtg acggtcgcgg acgggccgct
     2581 ggtcgaggac ggcgcggtct ggaccgcggc gagcggagcc tggggcgtgg ggctggggct
     2641 cgccgtcatc gcgaggctgt acgggcgcgc ggcggccaac cgcaccgcgg cggagttgga
     2701 cgcggcgtgg gacgagtcgc tgatcgcgcc gtgacatccg accggcgagc gcctataata
     2761 gctgcgccga tgcccgccat caaacacgtc cacctgatcg ccatctgcgg aaccggaatg
     2821 ggatcgctcg cggggatgct caaagcctcg ggctaccacg tgtcggggtc cgatcagaac
     2881 gtgtacccgc cgatgagcac gtggctggcg gagcagggca tcgtcccgta ttccgggttc
     2941 gcgcccgagc acctcgacgg ggcggacctg gtcatcgtgg gcaacgcggt ctccaaggcc
     3001 aaccccgagg tggtcgcgat gctcgagcgc ggcctcccgt accgctcgtt cccgcaggcg
     3061 cttgccgagt ttttcatccg ggaccggcat tccatggtcg tggccggcac ccacggcaag
     3121 accacgacca ccgcgctgct cgcgtgggtg ctgacctcgg ccgggcggga cccgggcgcc
     3181 atgatcggcg gctgggccaa gaacttcgac gggaatttcc gggtcggcgg ggggggcgtg
     3241 gtggcggtgg agggcgacga atacgacacc gcgtttttcg acaaaggccc caagttcctt
     3301 cactaccggc cgcgcaccgc gatcctgacc agcatcgagt tcgaccacgc cgatatctac
     3361 cgcgacctgg cgcacgtggt gtccgcgttc gaacggttcg tggagttgat cccgcgcgac
     3421 ggattgctgg tcgccgcggc gggcgaggag accgtggcgc gcgtcgcggg tcgcgcggcg
     3481 agccgggtcg aaacgtacgg cttcgccgcg gggagcgact ggtcggccga ggggctgtcc
     3541 gcccgggagg gcggcgtctc gttcgacgtg cgttacgccg gcgcgcggtt cggccggttc
     3601 gatctgccct tgatcggacg gcacaacgtg ctcaacgcgc tggccgtgat cgcggcggcg
     3661 cacggcgcgg ggctcgatgc gcgcgagatc gccgagggct tcaggacctt ccgcgggatc
     3721 aaacgacgtc aggacgtggt cggggtcgcg aacgacgtgg tggtgatgga cgacttcgcg
     3781 caccatccca ccgcgatccg ggagacgttg gccgcggtca agatgggcta cccgtcccgc
     3841 cagctctggg cgatcttcga accgcggtcg gccaccagcc ggcgcgcggt ctttcagacg
     3901 cagttcgcgg aggcctttga tctggccgat cgcgtggtga tcgccgagcc gttcgcgtcc
     3961 gagaaattgc cggtcgagca gcgcttggac gccaaacgcc tggtggacga tctggggcgg
     4021 cgcggcgttc acgcggcgca cctggcgaac gcggacgaca tcgtccgcac cgtggccccg
     4081 cagttggctc ccggcgacgt catctgcgtg atgtccagcg gcggcttcga cggcatccac
     4141 gccaaactgc tcgcggccct gcgggccgcc gaggtccgat cgctgaggcc gccgaagtcg
     4201 gcatgacccc gctgcgtaag ccgcccgcgc tgcgcaaggg agatctcatc ggcgtggtcg
     4261 cgccggccgg cgcggtgaaa accgaggacc tcaacgccgg cgtcaaacgc ctcgaagaac
     4321 tcgggtaccg cgtgctcgtg gggcggtcgg tgtaccgcgc ggcgcggtat ctggcgggcc
     4381 gcgacgccga gcgcgcgggc gatctcatgg aaatgttcgc gaacgccgag gtccgggcca
     4441 tcgtcgccgc ccggggcgga tacggcacgt cgcgagtgat cccgctgctc gacccccggg
     4501 ccatcgcgcg ttccccgaaa atcgtggtgg gcagcagcga cgtcacgctg ctgctgcatt
     4561 tcctgcaaac cgcctgcgcg ttcgtgacgt tccacgggcc gatggtcagc ccgaatttcg
     4621 gcaagtactc ctcgcccgcc accaacgact cgttcgtgcg cgtgctgggc gcgaccgcgc
     4681 cgcccggtcg gttcgacgtg cgcggggtga cgacgctcaa gggcggcagc gcggaaggcg
     4741 tgttgaccgg cgggtgcctg tcgctggtgt gtcagaccat cgggaccccg tacgagatcc
     4801 aaaccgacgg ggcgatcgtc tttctcgaag acatcaacga gccgccgtac cggatcgacc
     4861 ggatgttgac ttacctcaag caactcggca agttcgaccg ggcgcgcgcc gtgatcttcg
     4921 ggctcatgcc ggattgccat cccgcgcccc aggagtccta ccggctccac gacgtgattc
     4981 gggacgtgct cggcgacctg ccctgtccga ttctcctgga tttcccctcc ggccacggag
     5041 ggaccaacgt cacgctgccg ttcggggtgc gggcggccgt ggatggggcg acgctgagca
     5101 tcttggaagc gccggtcagt taggcgcgcc ccgcaacact ccccaaggtc ttcaggccgc
     5161 tcgaaacact cgcgacgctc ccggttccgc gaactcccgc ggctggacac agatccgcgc
     5221 ggctcgcaat gcaacacgaa cggggctgat tcaggacgga gctaggggca cgcgctgagc
     5281 gcgatcgaga tcgcggggct cgacacattg cccgccgcgt ccttggcaac caggctgatc
     5341 gcggccggct gatcatcgag ttcgatgacg ccggaccacg tggtctggcc cgtcgagcct
     5401 acgattgcct gatccgcggc ggtcggttcc atcacccgga acaccgcgct ctccgcgtcc
     5461 ttggttccag agagtgtgat gccacgcggc gagtcggtgg cggggtcgca ggccgtcagg
     5521 gtcacggatt gcggcggcgc ggggggggcg gtgtcgcgcg tgacctgata ggtggtctcg
     5581 gtgctgacca ggccgtcggc gtcgatcgcg accattcgaa acgtaaacag gcggtcggcg
     5641 gcgccggcga gcgggagcgt ggcggtccat ccggtgtcgt agtcgaacgg gacggtcacg
     5701 acgccgtcga tctccacgcg tgaccccacg ggtttcacgc ccgtgatgat cacgaagtcg
     5761 tcgttggtga tgatcggcgg ggtcgggcga aggtccgccg actgcgcggc gacttctgcg
     5821 gcgatgccgg tcgactgggc cacccggacg cgataggtgt acgtcggaca ggcgtcgccg
     5881 agcacggaac acatgcgcaa gcccacgtgc cgaaacggcg gcgcggagac cgtccgggtg
     5941 ccggcgatgg ggctgccgtc gacgtcctgg gcaaaccacg tgacgtcgta ggccgcggcg
     6001 ccgatgatct cgtcccacga gagggtattg acgccccggt tcgccgaaag ggtcacggtt
     6061 ttcggcacgc cgtcggccga agctttgggt acggcgtaca tggtgggcga cgcggggccc
     6121 agcccgcccg caaagagcgg gcggatccag atcgcgtggc gccgaccgtt ctcgagatct
     6181 ccgatggtga ggggaaatgt cggaaacggc gacacgtagg tgacttccga cctcgccacg
     6241 gagatcgccg cggtgtcgcc agccggccac gtgatggtga cctggccctg cgacgcggtg
     6301 gcggtcacgc cgccggtcgt ctgcgccgga atcgcctgga cctcggccga tgcggcgctt
     6361 tcaccgtcgg tccccacgac cgcggtgacc acgtagtaat acggagtccc gggatccagg
     6421 ccggtgtgcg tgtacgt
//
LOCUS       DBNGTT010000094         2189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2319522, whole genome shotgun sequence.
ACCESSION   DBNGTT010000094 DBNGTT010000000
VERSION     DBNGTT010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2319522"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="AC16BC_02165"
     CDS             <1..545
                     /locus_tag="AC16BC_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240449.1"
                     /translation="RRVYAGTDEKTALHKKIFLEAFDFDLFSINGVDLKRAAKALENG
                     DDRACRDCGNRTAGRMATFSYRKAFSINQFQTIWDRAHAVLRDSDLWLFIGYSMPEAD
                     FEFRHLLKSAQLARREHSSWSCKVILKRDQPDQQNSLSEAAAQYRRFFGIDSAMIYRG
                     GLSTWVQDQFDSFCSENGER"
     gene            652..1032
                     /locus_tag="AC16BC_02170"
     CDS             652..1032
                     /locus_tag="AC16BC_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016370.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR3240450.1"
                     /translation="MPGRALPPHLLQRCRRQHPHGSFIVDFYCHAARLAIELDGGVHA
                     LPEQSWRDSQRTRALEAEGIHVLRFWNHEVLKNTEGVLETILQARAFPHPSPLPRGEG
                     RDVVDDEKRLLNADVADRSVRGVE"
     gene            complement(1041..>2189)
                     /locus_tag="AC16BC_02175"
     CDS             complement(1041..>2189)
                     /locus_tag="AC16BC_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3240451.1"
                     /translation="MEVIAANAVILATGGYGRVFSLSTNALINTGDGMSMAYRAGAPL
                     MDMEMVQFHPTTLKRTGILISEGARGEGGYLLNAAGERFMANYAPNKLELASRDVVSR
                     AEQEEIDAGRGVNGCVLLDLRHLGRQKILERLPQIRELALTYVGVDAIEAPIPIRPGA
                     HYSMGGIKTDSWGRTAVPGLYAAGECACVSVHGGNRLGGNSLLDTIVFGRRGAQDAIT
                     QVRGKAAPRDVGRDALVRDRDRIEALRNRKSGERVAAVRDALGQIMSEDVGVFRTREK
                     LESAREALRAIRPRVESVMIQDKSRVFNTELISALELGSLMDLAETIVVGAIARQESR
                     GAHARRDFPTRDDANWLKHTMAYYTPSGPRLDYLPVTITKYPPKERTY"
BASE COUNT          381 a          677 c          678 g          453 t
ORIGIN      
        1 gccgaagagt ttatgcgggt actgatgaga aaacagccct tcacaaaaag atttttctgg
       61 aggccttcga cttcgacttg ttcagtataa atggggtcga tctgaagagg gccgccaaag
      121 ccctcgaaaa tggtgatgac cgcgcgtgtc gtgattgtgg gaaccgcacc gctggtcgca
      181 tggccacgtt cagctaccgc aaggcgtttt cgatcaatca gtttcaaacg atatgggacc
      241 gggcccatgc tgtcctgcgt gattcagatc tctggctgtt tatcggctac tcgatgccag
      301 aggccgactt cgagtttcgg catttgctta aatccgcgca acttgcccgc agggaacatt
      361 cctcctggtc gtgcaaagtc attcttaaga gggaccagcc agatcaacag aattcgctgt
      421 ccgaagccgc agcgcaatat cgccggtttt tcggtattga ctcagccatg atctatcggg
      481 gaggactctc cacttgggtt caggaccagt tcgactcatt ttgttcagaa aatggtgaac
      541 ggtgattccg aagaaaagta aacgcgcccg atgtggtcct ttcgctgttg tcgttaaggg
      601 gagaaagccc cttgctccct ctcccttggg gagagggttg gggtgaggga aatgcccgga
      661 cgcgcgctcc caccccatct gttgcaacgc tgccgacgcc agcatcctca tggaagtttt
      721 atcgtggatt tttactgtca tgcagcgcga ctcgcgatcg agcttgatgg cggtgtccat
      781 gcacttcctg aacagtcgtg gcgtgattcc caacggacac gcgcgctgga agcggaaggc
      841 atccacgtac tccggttctg gaaccacgag gttttgaaaa acacggaggg tgtgctggag
      901 accattttac aggcccgtgc cttccctcac cctagccctc tcccacgggg agagggaagg
      961 gatgtcgtcg acgatgagaa gcggttgttg aatgccgatg tcgccgaccg ctccgtgcga
     1021 ggcgtggaat agttggtgaa tcagtacgtc cgttccttcg gcgggtactt ggtaatcgtc
     1081 accggcaggt aatcgagccg cgggcccgac ggcgtgtagt acgccatcgt gtgtttgagc
     1141 cagttggcgt cgtcgcgagt gggaaagtcg cgccgtgcgt gggcgccgcg actttcctgc
     1201 cgcgcgatgg cgccgaccac gatggtctcc gccaagtcca tcaacgagcc caattccagg
     1261 gctgaaatca actcggtgtt gaacacgcgg ctcttgtcct ggatcatcac ggactccacc
     1321 cgggggcgga tggcccgtaa cgcctcgcgc gcgctctcca gtttttcacg ggtgcggaac
     1381 acgcccacgt cctcgctcat gatctgtccc agggcgtcgc gcaccgccgc cacccgctcc
     1441 ccggatttcc ggttgcggag cgcttcgatg cggtcgcggt cgcggaccag cgcgtcgcgg
     1501 cccacgtccc ggggcgcggc cttgccccgg acctgtgtga tcgcgtcctg cgcgccgcgc
     1561 cggccgaaca cgatcgtgtc cagcaacgag ttgccgccca ggcggttccc gccgtgcacg
     1621 ctcacgcacg cgcactcgcc ggccgcgtac aggccgggga ccgcggtgcg tccccacgag
     1681 tcggtcttga tgccgcccat gctgtagtgc gcgccggggc ggatgggaat cggcgcctcg
     1741 atcgcgtcga cgcccacgta ggtcaaggcc agttcgcgga tttggggcag gcgctccagg
     1801 attttttgcc ggccgaggtg tcgcagatcc aacagcacgc agccgttcac tccgcgcccc
     1861 gcgtcgatct cttcctgctc cgcgcgcgag accacgtcgc ggctcgccag ttccagtttg
     1921 ttgggcgcgt agttcgccat gaaccgctca cccgccgcgt tgagcaagta tccgccctcg
     1981 ccgcgcgcgc cctcgctgat caggatgccg gtgcgcttga gcgtcgtggg gtgaaactgc
     2041 accatctcca tatccatgag cggcgcgccc gcgcggtagg ccatgctcat gccgtcgccg
     2101 gtgttgatga gcgcgttggt gctgagcgag aacacgcggc cgtagccgcc cgtggccagg
     2161 atcacggcgt tggctgcgat gacctccat
//
LOCUS       DBNGTT010000095         5508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1041783, whole genome shotgun sequence.
ACCESSION   DBNGTT010000095 DBNGTT010000000
VERSION     DBNGTT010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1041783"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="AC16BC_02180"
     CDS             <1..277
                     /locus_tag="AC16BC_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3240452.1"
                     /translation="VLDAAAALNLYVMGSAALYQSRLAQDLPGEIRNRVTGFATDAQR
                     AVQFARSTPGIGTALVGMKAVGHVDENLGTLAAPVLSRADLDRLVPR"
     gene            361..741
                     /locus_tag="AC16BC_02185"
     CDS             361..741
                     /locus_tag="AC16BC_02185"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3240453.1"
                     /translation="MSESGELVNLKNVTEATWDAVVTSSELPVVVNFFGPACEHSQKL
                     GPVFGALSHRFVNKMRFAKVDVVAERGLAARYAVLSTPTLLFFVSGRPYFMAIGDAPK
                     HQLESELDHILAQNKRCMARSSPL"
     gene            965..2242
                     /locus_tag="AC16BC_02190"
     CDS             965..2242
                     /locus_tag="AC16BC_02190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR3240454.1"
                     /translation="MPLHPSPPSESVLAPIVLLLGPSPNRSTLTRALEHEGYRVEHAT
                     PGSDAAGRAAETGAALIILDGDGAARCADLRRDPRSHLVPILVLVGHTDWDERAAAIE
                     AGADAVFSYVPDHTRLLHTVDELIGAACPNEPPVALVFGDTRDSLNHLIDALQRAGVH
                     VLTCQNPADVFTVLGAKIPDAVIIGSRCGGLSGLDAYEALKRQDGLHHAPALFVTDEE
                     SHAERLVALRLGFDDYLHRADRHEIVRRVLSRVSRGRFFKHIANRDPLTGVLNYRAFI
                     DRLTHELERASRYDAPLTLILLDVDRFKQLNDRFGHLAGNRALQELVFFLRQRVRKSD
                     LIARLGGDEFAVLMIEAPKDAVASKWEHLSQGFRTTPLPLAGDESPAYVGFSFGMASW
                     PQDGDGIETLIARADTDLYRFKARARVATPDAA"
     gene            complement(2254..3078)
                     /gene="proC"
                     /locus_tag="AC16BC_02195"
     CDS             complement(2254..3078)
                     /gene="proC"
                     /locus_tag="AC16BC_02195"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3240455.1"
                     /translation="MTRRAIDRLGVLGAGRMGAALVRGALRAGLCTPDRVRVADPAVD
                     TVRSLTADTGVTASDNRHAVAASSLVVLAVKPQVIDGVLDDIRDAVTGGHLVVSIAAG
                     VPLARLAAHLPPKTRLARVMPNTPLLIGAGATAFAMGETAREEDAAAVETLFGALGLA
                     VRVPEEWLDAVTGLSGSGPAFVFSMIEALIDGGVKAGLPAETARALAVQTAVGAARLV
                     AETGSSPAALRDQVVSPGGTTQAGLTALQARGFREAVIAAVEAATLRSRELGRPRQ"
     gene            complement(3075..4112)
                     /locus_tag="AC16BC_02200"
     CDS             complement(3075..4112)
                     /locus_tag="AC16BC_02200"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3240456.1"
                     /translation="MAKKTKPQAVKELETVAAELSALAQASGDTDLFADLAGTILRLL
                     TEKADRGDVKLIARAFRELRYAFKTFTPYRNIRKVTVFGSARTKEDAPTYRQAVAFAR
                     GMVEQGYMVITGAGDGIMKGAQEGAGRERSFGVNIELPFEQLANEFIHADPKLIDCKY
                     FFTRKLIFVKESHALALFPGGWGTMDEGFEVLTLQQTGKSPPMPIVFVDEPGGSYWAE
                     WREYNVRHLLERGLISAEDLHLFAITDRPDVAVKEIMTFYKNYHSSRYVHGRLVLRLN
                     RPVPEALLDAINVEFADVVGQGKIEQGAALPEERNEHEVLHLPRLLLWFNRKNSGRLR
                     QLIDRINLGYE"
     gene            complement(4081..4557)
                     /locus_tag="AC16BC_02205"
     CDS             complement(4081..4557)
                     /locus_tag="AC16BC_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240457.1"
                     /translation="MSSGECSAAPSPEEKWAANRAKVEFAMTFPGLIREWTDAQGKTV
                     DTIVESGDTRVVIFTDHTFAFVPSRDLDPAAVVAALAAARPRLERVHAGAYAELDRLA
                     ARDRELSRRARLENILGAIRHNAADIPELKDAVRRLAASLPDHTETDGQEDQTAGR"
     gene            complement(4564..>5508)
                     /locus_tag="AC16BC_02210"
     CDS             complement(4564..>5508)
                     /locus_tag="AC16BC_02210"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR3240458.1"
                     /translation="RQFDAPFEQRLVVQKMVDAVLDVDPRYRTILEEPLDEAQTPEAA
                     FFAGSPVDSATARAFCAEFDAQLDGRTSKLVDYKAQNERVLADAVREVLGLPAARLSD
                     DHAIDLVLNPAQDRLLGESLNLTTHGKLTRAMVHPSYTFKKKLSHTADSQDQRHRMTP
                     ASRPVLAAHLFDAPDVIVPELIREEPALERDYHAVMAETWDAIGRLRRLGTPDEFAMY
                     LLPNAAAVRFTESADLLNLRHKHAMRLCYNAQEEIWRASLDEAVQVREVNPRIGRFLL
                     PPCTLRDMAEALPKCPEGERYCGVKVWRLDLSEFQRLV"
BASE COUNT          936 a         1908 c         1734 g          930 t
ORIGIN      
        1 cgtgctcgat gcggcggccg cgttgaattt atacgtcatg gggagcgcgg cgctgtatca
       61 gagccggctg gcgcaggatc tgcccggcga gattcggaac cgcgtcacgg gcttcgccac
      121 cgacgcgcag cgagccgtcc agttcgcccg gtccacgccc gggatcggca cggcgctcgt
      181 cgggatgaag gccgtcggcc acgtggacga gaacctgggg accctcgcgg cccccgtcct
      241 gtcccgagcc gatctcgatc gcctggttcc gcgttgacca atgggcactg caccccacaa
      301 ccacccgaca ggccctgacc gcatgggacc agggactgtt cagaggagga ggaaatccgc
      361 atgagcgagt ccggagagtt ggtgaacctc aagaacgtga ccgaagccac ctgggatgcg
      421 gtcgtcacgt cgtccgagtt gccggtcgtg gtcaatttct ttggacccgc gtgcgagcac
      481 agccagaaac tgggcccggt cttcggagcg ctctcccatc gattcgtgaa caagatgcga
      541 ttcgccaaag tcgatgtcgt cgcggaacgc ggcctcgcgg cgcggtacgc ggtgttgagc
      601 acacccaccc tgctgttctt cgtctcgggc cgaccgtatt ttatggcgat cggcgacgcg
      661 cccaaacacc agctcgaatc cgaattggat cacatcctcg cgcagaacaa acgctgcatg
      721 gcgagatcgt cgccgctcta atgcggggac gaacggcgca cggtccggca cgtgctcctc
      781 ggccgttgga ccaccaccca ccgtgaccct gcggtccgtg ccggaccggg gaatggacac
      841 attgcgaccc cgcgccgtcc gctccaccgc gccacgacca tgaggcgttg ttcagcctct
      901 gctatactct ccccgcaaca aatgagcccg cgccgcaccg acgtcacctg acaggagatc
      961 aaccgtgccg ctccatccgt ccccgccatc cgagagtgtc cttgcgccca ttgtgctcct
     1021 gctgggtcct tctccgaatc gttcgacgtt gacccgtgcg ctcgaacacg aaggctatcg
     1081 cgtcgagcac gcgacgcccg gttcggacgc ggctggacgc gcggccgaga ccggcgccgc
     1141 gttgatcatc ctcgacggag acggcgcggc gcggtgcgcc gatctgcgcc gggaccctcg
     1201 ctcccatctc gtcccgatcc tcgtgctggt cggccacacg gattgggacg agcgggcggc
     1261 cgcgatcgaa gccggagccg acgcggtgtt ttcgtacgtg ccggaccaca cgcgcctgtt
     1321 gcacaccgtg gacgaactca tcggcgcggc gtgtccgaac gaaccgccgg tggccctggt
     1381 gttcggcgac acgcgggact cgttgaatca cttaatcgat gcgctccaac gcgcgggcgt
     1441 tcatgtattg acgtgtcaga atcccgccga cgtctttacg gtccttggcg ccaaaatccc
     1501 cgacgccgta atcatcgggt cacgctgcgg agggctgagc ggattggacg cgtatgaggc
     1561 gctcaagcgc caggacgggc ttcatcacgc ccccgcgctg ttcgtgaccg acgaagagtc
     1621 ccacgcggaa cgcttggtcg cactgcgcct ggggttcgac gattacctgc accgcgccga
     1681 ccgccacgaa atcgtgcgcc gcgtgctcag ccgggtctcg cgcggtcgat tcttcaaaca
     1741 catcgccaac cgcgatccgc tcaccggcgt gctcaactac cgcgcgttca tcgaccgcct
     1801 cacccatgaa ttggaacggg cctcgcggta cgacgcgccg ctgaccctga tcctgttgga
     1861 cgtggaccgc ttcaaacaac tcaacgatcg tttcggacac ctggccggca atcgtgcgct
     1921 tcaagaattg gtgttcttcc tgaggcagcg ggtccggaaa agcgatttga tcgcgcgcct
     1981 cgggggagac gaattcgcgg tgctgatgat cgaagccccg aaagacgccg tggcgtcgaa
     2041 gtgggagcat ctctcacagg gtttccggac cacgccgctt ccgcttgccg gggacgagag
     2101 tccggcctat gtggggttct ccttcgggat ggcgtcgtgg ccccaagacg gcgacggcat
     2161 cgaaacgctg atcgccaggg ccgacaccga tctgtatcgg ttcaaggcgc gggcccgtgt
     2221 cgccacgccg gacgccgcgt aagtcgccgc gcgctactgc cggggacggc ccagttcccg
     2281 ggaacgaagc gtcgcggctt ccaccgcggc aatcacggcc tcgcggaatc ccctggcctg
     2341 caacgcggtg agcccggcct gcgtagtgcc tccgggggag acgacctggt cgcgcagcgc
     2401 ggccggcgac gaaccggtct cggccacgag acgcgccgcg cccaccgcgg tttgcacggc
     2461 cagcgcgcgc gcggtttcgg ccggcagtcc ggccttgacc cctccatcaa tcagcgcctc
     2521 gatcatggag aagacgaacg ccgggccgct cccgctcaaa ccggtcaccg catcgagcca
     2581 ctcctccggg actcggacgg ccagccccaa cgcgccgaac aacgtctcca ccgcggccgc
     2641 atcctcctcg cgcgccgtct cacccatcgc gaacgcggtg gcgccggcgc cgatcaacaa
     2701 cggcgtgttg ggcatcaccc gcgccaagcg ggtcttcggc ggcaggtgcg cggccaatcg
     2761 cgccagcggc acccccgcgg cgatcgacac caccagatgc cccccggtca ccgcgtctcg
     2821 gatgtcgtcg agcacgccgt cgatcacctg gggcttgacc gccagcacca ccagcgacga
     2881 tgccgcgacc gcgtggcggt tatcggacgc ggtcacaccc gtgtccgccg tcagggatcg
     2941 gaccgtatcg acggccggat cagccaccct cacccgatcc ggagtgcaca acccggcgcg
     3001 gagggctccc cgcaccaatg ccgcacccat gcgacccgcg ccaagtacgc ccaaccgatc
     3061 gatcgcgcgc ctggtcattc gtacccgagg ttgatccggt cgatgagttg acgcaaccga
     3121 ccggagttct tccggttgaa ccacaacagc agacggggca ggtgcagcac ctcgtgttcg
     3181 ttgcgctcct cgggtaatgc cgcgccctgc tcgatcttgc cttgacccac cacgtcggcg
     3241 aactccacgt tgatcgcgtc cagcaaggcc tccggcaccg gtcgattcag ccggagcacc
     3301 aatcggccgt gcacgtagcg cgaggaatgg tagttcttat agaaggtcat gatctccttg
     3361 accgcgacgt ccggtcgatc cgtgatggca aagaggtgca aatcctccgc cgagatcagc
     3421 ccgcgctcca acaggtgacg gacgttgtac tcgcgccact cggcccagta ggacccgccg
     3481 ggttcgtcca cgaacacgat gggcatgggc gggctcttgc cggtctgctg cagcgtgagc
     3541 acctcgaacc cctcgtccat ggtgccccac ccgcccggga ataatgcgag cgcgtgactc
     3601 tctttgacga agatcaactt ccgggtgaag aaatatttac agtcgatcaa cttggggtcg
     3661 gcgtggatga actcattggc caactgctcg aatggcaact cgatgttcac gccgaagctg
     3721 cgctcgcgtc cggccccctc ttgcgccccc ttcatgatgc cgtccccggc gccggtgatc
     3781 accatgtagc cctgctccac catgccgcgc gcgaacgcca cagcctggcg gtaggtgggc
     3841 gcgtcttcct tggtgcgcgc ggagccgaac accgtgacct tgcggatatt gcggtagggc
     3901 gtgaaggtct tgaacgcgta ccgcagctcg cggaacgcgc gggcgatcaa cttgacgtcg
     3961 ccccggtcgg ccttttccgt gagcaaacgg aggatcgtcc ccgcgagatc cgcgaacagg
     4021 tccgtgtccc cggacgcttg cgccaacgcc gacaattcgg cggcgacggt ctccagctct
     4081 ttaacggcct gcggtttggt cttcttggcc atcagtctcc gtgtggtcgg gcaacgatgc
     4141 ggccaaccgg cgcaccgcgt ctttgagttc ggggatatcc gccgcgttgt ggcggatcgc
     4201 ccccagaatg ttctccagcc tggcgcgccg cgacaactcg cggtcgcgcg ccgccaagcg
     4261 atcgagctcg gcatacgccc cggcatgcac gcgctccagc cgcggcctcg ccgcggccaa
     4321 cgcggccacc acggccgcgg gatccaggtc ccgcgatgga acgaacgcga acgtgtggtc
     4381 cgtaaaaatc acgacgcgcg tgtccccgct ctcgacgatc gtgtccacgg ttttcccttg
     4441 cgcgtcggtc cattcgcgga tgaggccggg aaaggtcatg gcgaactcga ccttagcccg
     4501 attggccgcc cacttctcct cgggcgacgg cgcggcggag cactctccag aactcacgga
     4561 atcctagacc aaccgctgga attcggataa atcgaggcgc cacaccttca cgccgcaata
     4621 tcgctcccct tcagggcatt tagggagggc ctcggccatg tcgcgcaggg tgcagggcgg
     4681 caggaggaat cggccgatgc gcgggttgac ctcgcggacc tggacggctt catccagcga
     4741 ggcgcgccag atttcctcct gcgcgttgta gcagaggcgc atggcgtgtt tgtggcgaag
     4801 gttcaacaaa tcggccgatt cggtaaagcg caccgccgcg gcattgggca acagatacat
     4861 ggcgaattcg tcgggcgttc ccaaccggcg caaccgcccg atcgcatccc acgtctcggc
     4921 catcaccgcg tggtagtcgc gttccaacgc cggctcctcg cgaatcagtt ccggcacgat
     4981 cacgtcgggc gcgtcgaaga ggtgcgccgc gagcacgggc cgcgacgcgg gcgtcatgcg
     5041 gtggcgctgg tcctgcgagt ccgcggtgtg gctcagcttc tttttgaacg tgtacgacgg
     5101 gtggaccatg gcgcgcgtga gtttgccgtg cgtggtgaga ttgagcgatt cgcccaacaa
     5161 tcgatcctgg gccggattaa gaaccaggtc gatcgcgtga tcgtcgctca accgcgccgc
     5221 gggcagcccg agcacctcac gcaccgcgtc ggccagcacg cgttcgttct gcgccttgta
     5281 atcgaccagc ttggacgtgc ggccgtcgag ctgcgcgtcg aactccgcgc agaaggcgcg
     5341 ggccgtggcg gaatcaacgg gcgaccccgc gaaaaacgcg gcctccggcg tttgggcttc
     5401 atccagcggt tcttcgagaa tcgtccggta gcggggatca acgtccagca ccgcgtcgac
     5461 catcttctgc accaccaggc gttgctcgaa cggcgcgtcg aactggcg
//
LOCUS       DBNGTT010000096         3412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_148062, whole genome shotgun sequence.
ACCESSION   DBNGTT010000096 DBNGTT010000000
VERSION     DBNGTT010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_148062"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="AC16BC_02215"
     CDS             <1..780
                     /locus_tag="AC16BC_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3240459.1"
                     /translation="PFVAVNCAALSEGLLESELFGHERGAFTGALFQKKGRFELADRG
                     TLFLDEIAELSPSLQVKLLRVLQEKSFERVGGTKTITVDVRILAATNREIRREIAERR
                     FREDLFFRLNVVTISLPALRERLDDIPELVEYFLNRYSTALHKPVTVAPEVLTGLMSY
                     AWPGNIRELENLIERLIVLADGDRIEYADLPEEIFGPAPQSTAADGLSDRISQLEKEM
                     IRRTIEDCGHNQTRAAKLLGLKRSSLQYKLKKYGLHSPPAS"
     gene            896..1243
                     /locus_tag="AC16BC_02220"
     CDS             896..1243
                     /locus_tag="AC16BC_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3240460.1"
                     /translation="MAAMKTATYVLRMVVTALGGLAILPLPALAAGDIQRGKAVYEKN
                     CQGCHGRAGQGVGGATPSLADPARMSALTDQDLFEAVTKGRPGTGMPSWNSILSEQDR
                     RDVVGYLRTFSRR"
     gene            1343..2356
                     /locus_tag="AC16BC_02225"
     CDS             1343..2356
                     /locus_tag="AC16BC_02225"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3240461.1"
                     /translation="MSAPRLLVIDHDPEVQHAVETALGPEGFEVTAVGDGLSALDVAL
                     ATTPDVVLAEYRMEGINVFRFLEKLKHKNSLKSVALLLLVSPGDVYDELTLRLVGVTD
                     FLPKPLNPKETLERVKRYRPLPLSVPASSAIPKPTEQAPGKIEDLLGWSQAAAPSPFS
                     ELSQDHAIGMDFNLAGSDAPENAPESIEATEFLARNEITPSDHPVVANETEQAFAELQ
                     DGPLAPSVPVFEAPVDPLFGEAEPSSPTAVSPASEPPSQPGPARNGSSGPQPELVERL
                     ARNIARDAVEKVAWDVVPDLAKQSVERVITAVVERVVWDIVPTIAEAAVKQEIERLTR
                     DNG"
     gene            2468..>3412
                     /locus_tag="AC16BC_02230"
     CDS             2468..>3412
                     /locus_tag="AC16BC_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3240462.1"
                     /translation="MVMADLDTVYHPKDVEARWTRFWLERDLFRADPASTGTPYCVVI
                     PPPNVTGSLHVGHALNNSLQDILVRWHRMRGDATLWQPGTDHAGIATQNVVERQLKAE
                     GLSRDDLDRKQFIERVWAWKQESGGRIVEQLKRLGASCDWSRERFTMDAGLSAAVREV
                     FVRLYHEGLIYRAERLINWCWRCGTALSDIEVEHEEVAGKMYYIRYPLAEDPTQSLTV
                     ATTRPETLLGDTAVAVHPDDPRFNAWIGRRVALPLTHRTIPVVGDPILVDREFGTGAV
                     KITPGHDFNDEKAGRRHNLDVVSLLNSRGRIDRTSYPAV"
BASE COUNT          676 a         1093 c         1018 g          625 t
ORIGIN      
        1 cctttcgtag cggtcaactg cgcggcgctg tcggaggggc tcctcgagag cgagttgttc
       61 ggccacgagc ggggggcgtt caccggtgcg ttatttcaaa agaaaggccg attcgaactg
      121 gcggaccgag gcaccttgtt cttggacgag attgcggaac tgtcgccatc cctccaggtg
      181 aagcttctcc gcgtgttgca ggaaaagagc ttcgagcggg tcgggggcac caagacgatt
      241 accgtcgacg tgcggatcct cgccgccacc aaccgcgaga ttcgtcggga gatcgccgag
      301 cgccggtttc gagaagacct gttcttccgc ctcaacgtgg tgacgatctc ccttcctgcg
      361 cttcgggagc ggctcgatga tattccggag ctcgtggagt atttcctcaa ccgttactcc
      421 accgcattgc acaaaccagt gacggtggca ccggaagtgc tgaccgggtt gatgtcgtac
      481 gcgtggccgg gcaacatccg cgaattggag aatctcatcg aacgactcat cgtgttggcg
      541 gacggcgatc gcatcgagta cgcggatctc ccggaggaaa tcttcggccc ggcgcctcaa
      601 tccacagcgg cggacggcct ctcggaccga atcagccaac tggaaaagga gatgatccgt
      661 cggaccattg aagactgcgg tcacaaccag acccgtgccg cgaaactgct tggcctcaag
      721 cgcagctccc tgcagtacaa attgaaaaag tacggactcc actcaccgcc agcgtcataa
      781 tctgcccgtt cgtcgcaaaa agctcacgag aagagccatc agcaccccca ttggatatca
      841 ccgtggcgcc gcttctcgcg gaaatcgctt gacaggtctg gcacagcgcg agagaatggc
      901 cgcgatgaag acggcaacat acgttctgcg tatggtcgtc accgcactgg gcggcttggc
      961 gatccttccc cttccggcac tcgcggccgg ggacattcaa cggggaaaag ccgtgtacga
     1021 aaagaattgc cagggctgcc acggccgcgc cgggcagggt gttggcggag cgaccccgag
     1081 cctcgctgac ccggcccgca tgtcggccct caccgaccaa gaccttttcg aggcggtcac
     1141 caaaggccgg cctggaaccg ggatgccctc ctggaattcg atcctgtcag agcaggaccg
     1201 ccgggatgtc gttggttatt tgaggacatt ctcgcgacgg taacgacgcg cctcaggcct
     1261 tcctgccttt ggtcccgcgt ttgacaagct ataccacgaa tgctagctta aggaccgtgt
     1321 cgtctttcct tggtaaccta caatgagtgc acctcgactc ctcgtaatcg atcacgatcc
     1381 tgaagtgcag cacgcggtcg aaaccgctct cggccccgaa ggattcgagg ttaccgctgt
     1441 cggggacggc ctgagcgctc tggatgtcgc cttggccacc acaccagacg tcgtgctggc
     1501 ggaataccga atggaaggca tcaatgtctt tcgattcctt gaaaaactga agcacaagaa
     1561 ctccctgaag agcgtcgcgc tgctcttgct cgtcagcccc ggggacgtct acgatgagtt
     1621 gacgctgcgc ttggttggcg tgactgattt cctcccaaag ccgctgaatc ccaaggaaac
     1681 gctggagcgc gtcaaacggt accgcccgct tcccctttcc gtccctgcat cctcggcgat
     1741 ccccaaacca acggaacaag cgccgggaaa gatcgaggac ttgctgggat ggtcgcaagc
     1801 cgcggcgccc tcgccgtttt cagagctgtc gcaggaccac gccatcggga tggatttcaa
     1861 cctcgcggga tcggacgcgc cagaaaacgc tccggaatcg atcgaggcca ctgaattctt
     1921 ggcgcgcaat gaaatcactc ccagcgatca tccggtggtg gccaacgaga ccgagcaagc
     1981 gttcgccgag ttacaggatg gcccgctcgc gccatccgtg cccgtattcg aagcgccggt
     2041 tgaccccctt tttggggaag ccgaaccctc ctcccccacg gcagtctcgc cggcttcgga
     2101 accgcccagc caaccaggac ctgcgcggaa cgggagttcg ggcccgcaac cagagcttgt
     2161 ggaacgcctc gcacggaaca ttgctcgcga cgcggtggaa aaggtggcgt gggatgtcgt
     2221 gccggacctc gccaaacaat cggtggaacg cgtgatcacc gccgtggtcg aacgcgtggt
     2281 ctgggacatc gtccccacca tcgccgaagc cgccgtcaag caagagatcg agcgcttgac
     2341 gcgggacaac ggttagcccg gtcctcgggc caaccgctct ctactatccc atcaagaaat
     2401 atctgatccg cggtttcttt acaagtttta cggggcgatg gtatgatccc cgcgttctcg
     2461 tccccgcatc gtcatggccg acctcgacac cgtgtaccac cccaaagacg tggaagcccg
     2521 ctggactcgc ttctggcttg agcgcgacct ctttcgggcg gacccggcat cgacaggcac
     2581 gccgtactgc gtcgttattc cgccgccgaa cgtcacgggg tcgctgcacg tcggccacgc
     2641 actgaacaac tcgctgcagg acattctggt tcgctggcac cgcatgcgcg gcgacgccac
     2701 gctgtggcag cccggaacgg atcacgccgg catcgcgacg cagaacgtcg tcgaacgcca
     2761 gctgaaggcc gaaggcttgt cacgggacga cctcgaccgg aagcagttta tcgaacgcgt
     2821 gtgggcgtgg aagcaggagt ccggcggccg gatcgtggaa caactcaagc ggctcggcgc
     2881 gtcgtgcgat tggagccgcg aacgcttcac catggacgcg gggttgtccg ccgcggtccg
     2941 cgaagtcttt gtccgcttgt accacgaggg tctgatctac cgagccgaac gcttgatcaa
     3001 ctggtgttgg cgttgcggaa ccgcattgtc cgacatcgag gtcgaacacg aggaggtcgc
     3061 gggaaagatg tactacatcc gctaccccct cgccgaagac ccgacgcagt ccctcaccgt
     3121 cgccacgacc cgtcccgaaa ccctgctcgg agacacggcc gtcgccgtcc atcctgacga
     3181 cccgcgattt aacgcgtgga tcggccgacg tgttgccctg cccctgaccc accggacgat
     3241 tcccgtggtc ggcgacccga ttttggttga ccgcgagttt ggaaccggcg cggtcaagat
     3301 cactcccggg cacgacttta acgacgagaa ggcggggcga cggcataacc tcgacgttgt
     3361 ttcgctcctc aattcccgag ggcgaatcga ccgcaccagt taccccgcgg tg
//
LOCUS       DBNGTT010000097         4938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_148139, whole genome shotgun sequence.
ACCESSION   DBNGTT010000097 DBNGTT010000000
VERSION     DBNGTT010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_148139"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..618
                     /locus_tag="AC16BC_02235"
     CDS             184..618
                     /locus_tag="AC16BC_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3240463.1"
                     /translation="MAQIIVRELMTPNPITVEHGQTVGKAAELMARFDVRRLPVLREG
                     KLVGIISDRDVRQMSGRPSVKLQKTAADEAYLQLPVEEAMTLNVITIRESQPVKDAIA
                     LLLKHKIGGLPVLGRDGGLVGVLSEQDVLKHCLGILEREADR"
     gene            complement(637..912)
                     /locus_tag="AC16BC_02240"
     CDS             complement(637..912)
                     /locus_tag="AC16BC_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240464.1"
                     /translation="MSPEERERIKKLVRELSQCVNSALTDSSDVKDALKDIEEEGYQV
                     DLLFALFTRILRKKDDTPQLEAPPLSADDSAFDEQFLKTLRLNWSNE"
     gene            1057..2853
                     /gene="lepA"
                     /locus_tag="AC16BC_02245"
     CDS             1057..2853
                     /gene="lepA"
                     /locus_tag="AC16BC_02245"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR3240465.1"
                     /translation="MLETTRNFSIIAHIDHGKSTLADRLLEQTGALTARELREQVLDD
                     MDLERERGITIKAHAVRLRYRAADGRTYDFNLIDTPGHVDFTYEVSRSLAACEGALLI
                     VDATQGVEAQTIANAQLAFSSNLAIVPVINKIDLPSADVDKVKREIEDVLTIDASEAI
                     LASAKEGIGISEILEAIVRRIPPPRGDDAAPLKALIVDSWFDNYQGAIILARVMDGRV
                     RAGTKILLMATGKTYIVDSVGVFTPKRLPTEELSAGDVGYIVAGIKNVADTKIGDTVT
                     DAARPAAEPCPGYTEVKPMVFCGLYSVDSADFEALRDALEKLRLNDSSFSFEPETSLA
                     LGFGFRCGFLGLLHMEIIRERLEREYHLALISTAPTVVYRVTTLKGEVVHIENPAKLP
                     DVQKIATFEEPMIRATIITPEEYLGALLRLCEERRGTQLDMKYLDVKRLMLTYRLPLN
                     EVILDFYDRLKSISRGYASFDYEVIGYEPSDLIKLDILLNGETVDALSCIAHRSKAAT
                     RGRQLVEKMRELIPRQMFEIAIQAAIGSRVIARETVGALKKNVTAKCYGGDISRKRKL
                     WEKQKEGKKRMKQVGRVEIPQEAFLALLKVTD"
     gene            2862..3467
                     /gene="lepB"
                     /locus_tag="AC16BC_02250"
     CDS             2862..3467
                     /gene="lepB"
                     /locus_tag="AC16BC_02250"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3240466.1"
                     /translation="MGARKKSVVREYAEAFALAILLALAIRTFVVQAFKIPSGSMIPT
                     LSIGDHILVNKFIYGVKIPFTDQIVIPVSLPERGDIIVFKFPGDESKDYIKRVVGTPG
                     DVVEVRNKAVYVNGTLADTTHIQHIDPEVHQDRRDNFGPATVPPDSYFVMGDNRDQSL
                     DSRFWGFVKLNKIRGKAFLVYWSWDGADTWVRWSRIGRVIQ"
     gene            3532..4536
                     /gene="rfaE1"
                     /locus_tag="AC16BC_02255"
     CDS             3532..4536
                     /gene="rfaE1"
                     /locus_tag="AC16BC_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HIR3240467.1"
                     /translation="MTGDAARLRGWATGLSGARVAVLGELMLDRFIWGRVSRISPEAP
                     VPVVEVTSESVLLGGAGNVFLNLQSLGASVSLCGVVGDDAPGSALIDLLRARGASLDG
                     VLAESGRPTTQKTRVIAHQQQVVRVDRERRAPLAAKTRDAIIEAVDAALDRANCLVVS
                     DYAKGVVSPELMDRVRTAASRRGVEILVDPKVSNMPCFTGVHVVTPNHLEASQASGVE
                     IVDAETLAQAGRTLVERFRIAAVLITRGEHGMSLFERNGGVTHIPTVAKQVFDVTGAG
                     DTVIATLAAARSIGAPLADAARLANCAAGYVVGVVGTAAVTPDALRRQLDEYLAEVPA
                     "
     gene            4533..>4938
                     /locus_tag="AC16BC_02260"
     CDS             4533..>4938
                     /locus_tag="AC16BC_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014409.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR3240468.1"
                     /translation="MKVLVVIPARYASTRLPAKPLADIGGKSMIQRVWEQGRAARGVE
                     DVVVATDDERIVRVVQAFGGRAVMTSDAHASGTDRLAEVASRHHADVYVNLQGDLPFA
                     PPAMIEAVVRAFDDPSVRLSTLARAITDAADVY"
BASE COUNT          912 a         1515 c         1644 g          867 t
ORIGIN      
        1 atacgcgcag gaacggaatc cgcaacacga cctcgtggac atcggctaag tgctcgcgca
       61 gatcccgcgc gtagacgggg cgacacgaca ttcgggttga aaccgactgc ggggttgggt
      121 gaagatcacc cacggtccgc cgtacggcca gacggacgac tcacatgagg ggataagacg
      181 cccatggcac agatcattgt tcgggaactc atgacgccca accccatcac cgtcgagcac
      241 ggccagacgg tcggcaaagc cgcggaactg atggcgcggt ttgatgtccg gcgcctcccg
      301 gtcctgcggg agggcaaact ggtcggcatc atttcggacc gcgacgtgcg ccagatgtcc
      361 gggcgcccga gcgtcaagct ccagaaaacc gcggcggacg aagcctatct gcagttgccg
      421 gttgaagagg ccatgacgtt gaacgtgatc accatccggg agagtcaacc cgtgaaggac
      481 gcgatcgcgt tgctcctcaa acacaaaatc ggcggacttc ccgtgcttgg ccgcgatggc
      541 gggctcgtcg gagtcctgtc ggaacaggac gtcctcaagc actgtctcgg tatcctggag
      601 cgagaagccg accggtgacg gcttcgaggc ggggcgttac tcgttggacc agttcaggcg
      661 cagcgttttg aggaactgct cgtcgaacgc cgagtcgtcg gccgacaacg ggggcgcttc
      721 gagctgcggg gtgtcgtcct tttttcgcag aatgcgggta aacagcgcaa agagcaggtc
      781 gacctggtag ccctcttcct cgatgtcctt gagcgcatcc ttcacgtcgc tggaatcggt
      841 caacgctgag ttgacgcatt gcgacaactc gcgaaccagc tttttgatgc gttctcgttc
      901 ctccgggctc atccgtgtcc cctcgcagtg tgagaaccag cttatcccac gaacggctgg
      961 tgtcaagacg acgcgcgcag ggcctcatgt tctttgactt tggtccctga aattcctata
     1021 atttgacgat ttttcgacca tccgcgcaga accccgttgc ttgaaaccac cagaaatttt
     1081 tcgattattg cccatatcga ccatgggaaa tcgacgcttg cggaccgttt gctcgagcag
     1141 accggcgccc tgaccgcgcg cgagctgcgt gaacaggtcc tggacgacat ggatctggaa
     1201 cgcgaacgcg gaatcacgat caaggcgcac gcggttcggt tgcgctaccg cgctgccgac
     1261 ggccgcacct acgatttcaa cctgatcgac acgccgggcc acgtggactt cacctacgag
     1321 gtgtcgcgga gccttgccgc gtgcgagggc gcgctcctga tcgtggacgc gacccagggc
     1381 gttgaggcgc agaccatcgc caacgcgcag ctcgcgttct ccagcaacct cgccattgtc
     1441 ccggtcatca acaagatcga cctgcccagc gccgacgtgg acaaggtcaa gcgcgagatc
     1501 gaagacgtgc tgaccatcga cgcgtccgag gcgatcctgg cgagcgccaa ggaaggcatc
     1561 gggatctcgg agattttgga ggcgatcgtg cgccgcatcc cgccgccgcg cggcgatgac
     1621 gcggcgccgc tcaaagcgct gatcgtggac tcctggttcg ataattatca gggcgcgatc
     1681 attctcgcgc gcgtgatgga cggccgcgtc cgggccggca ccaagattct cctcatggcg
     1741 accggcaaga cctacatcgt ggattcggtc ggcgtgttca cgcccaagcg tttgccgacc
     1801 gaggagttgt ccgcggggga cgtgggctac atcgtggccg ggatcaagaa cgtggcggac
     1861 accaagatcg gagacacggt caccgacgcg gcgcgtccgg ccgccgagcc gtgcccgggg
     1921 tacaccgagg tcaagcccat ggtgttctgc ggcctctaca gcgtggacag cgcggacttc
     1981 gaggcgctgc gagacgcgct ggaaaaactg cggctcaacg actcgtcgtt ttccttcgag
     2041 cccgaaacgt cgttggcgct cggcttcggg tttcggtgcg gattcctcgg cctgctgcac
     2101 atggagatca tccgcgagcg gttggaacgc gaataccacc tggccttgat cagcaccgcg
     2161 cccaccgtgg tctaccgggt gacgactctt aagggcgagg tcgtgcacat cgaaaatccg
     2221 gccaagctgc cggacgtgca gaagatcgcc acgttcgagg agccgatgat ccgcgccacc
     2281 atcatcacgc cggaggagta tctgggcgcg ttgctgcggc tgtgcgaaga gcggcgcggc
     2341 acgcaactcg acatgaaata tctggacgtc aaacgcctga tgttgacgta ccggttgccg
     2401 ctcaacgaag tgatcctgga tttttacgac cgcctcaagt cgatcagtcg cgggtacgcg
     2461 tcgttcgatt acgaggtcat cgggtacgaa cccagcgatc tgatcaaatt ggatattctc
     2521 ttaaacgggg aaaccgtgga cgcgttgtcc tgcatcgcgc accgcagcaa ggccgcgacc
     2581 cgcggccgcc aactggttga gaagatgcgg gaactgatcc cgcggcagat gttcgagatc
     2641 gcgatccagg ccgcgatcgg gagccgcgtg atcgcgcgcg aaaccgtcgg tgcgctcaag
     2701 aaaaacgtca ccgccaagtg ttacggcggc gacatcagcc gcaaacggaa gctatgggaa
     2761 aaacagaagg aaggcaagaa gcgcatgaaa caagtcggcc gggtcgagat ccctcaggaa
     2821 gcgttcctcg cgctcttgaa agtgaccgac tgaccccgca catgggggcg cgcaagaaat
     2881 ccgttgtccg cgagtacgcg gaagcctttg cgctggcgat cctgctggct ctggcgatcc
     2941 ggaccttcgt ggttcaggcg ttcaagatcc cgtcggggtc catgatcccg acgctttcga
     3001 tcggcgacca catcctcgtc aacaagttca tctacggggt gaagatcccg tttacggacc
     3061 agatcgtcat tccggtgtcc ctgcccgagc ggggagacat catcgtcttc aagtttccgg
     3121 gagacgagag caaggactac atcaaacgcg tggtgggcac cccgggcgac gtggtggaag
     3181 tgcgcaataa agcggtgtac gtcaacggca cgttggcgga caccacgcat atccagcaca
     3241 tcgatcccga ggtccatcag gaccggcggg acaatttcgg gcccgcgacc gtgccgccgg
     3301 actcctactt cgtgatggga gacaatcggg atcagagttt ggacagccgg ttctggggtt
     3361 tcgtcaagct caacaagatc cggggcaaag cgtttctcgt ctattggtcg tgggacggcg
     3421 ccgacacgtg ggtgcgatgg tcccggatcg gacgggtgat tcagtgatcc gacttgtggt
     3481 acgctgcggc cgcgacggac gttcggacaa cgtcatcggg agggacacgg cgtgacgggg
     3541 gacgcggcgc gattacgcgg gtgggccacg ggattgtcgg gagcccgggt ggcggtactg
     3601 ggcgagttga tgctcgaccg cttcatctgg ggccgcgtct cgcgcatttc acccgaggcg
     3661 ccggttccgg tggtcgaggt cacctcggag tcggtgctcc tcggcggcgc cggcaacgtg
     3721 ttcctgaacc tgcagtcgct gggcgcctcg gtgtcgctgt gcggggtggt gggcgatgac
     3781 gcgcccggct ccgcgttgat cgacctcttg cgggcgcgcg gcgcgtcgct cgacggcgtg
     3841 ctcgccgaga gcggacggcc gaccacccaa aaaacccggg tgatcgcgca ccaacagcag
     3901 gtggtgcggg tggaccgcga acgccgcgct ccgctcgcgg ccaagacccg cgacgcgatc
     3961 atcgaggcgg tggacgccgc gctcgatcgc gcgaattgcc tggtggtctc ggactacgcc
     4021 aagggcgtgg tctcgcccga actgatggat cgcgtgcgaa ccgccgcgtc ccgacgcggg
     4081 gtcgaaatcc tcgtggatcc caaggtttcg aacatgccgt gttttaccgg cgtgcacgtg
     4141 gtcaccccca atcatctcga agcctcccag gcgagcgggg tggagatcgt ggacgccgaa
     4201 acgttggcgc aggccgggcg cacgctggtc gagcgattcc ggatcgcggc cgtcctcatc
     4261 acgcgcgggg aacacggtat gtccttgttc gaacgcaacg gcggggtgac tcacattccg
     4321 accgtggcga aacaagtgtt cgacgtgacc ggagccggcg acaccgtgat cgccacgctg
     4381 gcggccgcgc gttccatcgg cgcgccgctc gcggacgcgg ctcgactggc caactgcgcg
     4441 gccgggtacg tggtgggcgt ggtcgggacc gcggccgtga ctcccgacgc cttgcggcga
     4501 caactcgacg agtatctggc ggaggtgccg gcgtgaaggt gctggtggtc atccccgcgc
     4561 gttatgcctc gacgcggttg ccggccaaac ccttggccga catcggcggc aagtccatga
     4621 tccagcgggt gtgggaacag ggacgcgccg cgcgaggggt ggaggacgtc gtggtggcca
     4681 cggacgacga gcgcatcgtc cgcgtggtgc aggcgttcgg tggccgggcg gtgatgacct
     4741 cggacgcgca cgcgagcggg accgatcggc tggcggaggt ggccagccgc catcacgcgg
     4801 acgtgtacgt gaatcttcag ggcgatctgc cgttcgcgcc gcccgcgatg atcgaagcgg
     4861 tcgtccgcgc gttcgacgac ccgtcggttc ggctctccac gctggcccgc gcgatcaccg
     4921 atgccgcgga tgtctaca
//
LOCUS       DBNGTT010000098         3916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2703589, whole genome shotgun sequence.
ACCESSION   DBNGTT010000098 DBNGTT010000000
VERSION     DBNGTT010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2703589"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="AC16BC_02265"
     CDS             complement(<1..213)
                     /locus_tag="AC16BC_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240469.1"
                     /translation="MLRFARMGAIVLCLALSAPASWASPPIESVLPNGLRVILIPEAK
                     APVVSVQVWYRVGSRDEITGKTGLSHL"
     gene            382..603
                     /locus_tag="AC16BC_02270"
     CDS             382..603
                     /locus_tag="AC16BC_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240470.1"
                     /translation="MQCSRCRGLMVVDRFTDLLDETGQITFGGWRCLACGEIVDPVIM
                     SNRQRKVALPTSKPRHTRYLQWIGRPPSA"
     gene            complement(931..1521)
                     /locus_tag="AC16BC_02275"
     CDS             complement(931..1521)
                     /locus_tag="AC16BC_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3240471.1"
                     /translation="MAVEYLPVAEAAEVPKGGVKVVTVAGTPIALLNVGGEIFALEHS
                     CPHQGGPIGEGEICGMTITCPWHEWKFDIRTGANDKDPTILAKTYAVRVAEGLVLVEV
                     SRVLAGARRNREILRRLTAGEPPEAIGRDLGLSEQEVTRTADAARISERLHYLAEAYL
                     RKGRVGGSDVRGMPYRRSKEADGEMLSALDALTRLL"
     gene            complement(1603..1752)
                     /locus_tag="AC16BC_02280"
     CDS             complement(1603..1752)
                     /locus_tag="AC16BC_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240472.1"
                     /translation="MNAITPPLLRQARGGLRRIDVARWALLRHARSGLRRIDMARHYV
                     ARWAF"
     gene            1791..3047
                     /locus_tag="AC16BC_02285"
     CDS             1791..3047
                     /locus_tag="AC16BC_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3240473.1"
                     /translation="MTTPHAIPADLQADLVIVGGGMGGAVLALLSAAKRRRVVVLDRQ
                     PTGPTDPNRGEIVQPNGLAVLDRLGLLAGLPATGVSRVRRYHFSRIGGSRLVTVDYGA
                     LPAPYNYTLVGHPRALMTTVRARLAESPFIEMRWGAQVVGLVRERRRVVGVEADLDGV
                     RTRVLASVVVGGDGSASPVRAALRIRTRVRRYADGYLTVVLPRPAGFGEDGRYYVGRR
                     EILGVFPLGDDFVYAFYLWPAGQREALEARGLPAFTSRIAAIDPDLRGALDALTRWDQ
                     VGWMPCVKVTPRAWVADGAALLGDAAHALNPHVAQGRNQALEDAVALDAVLDSCFAAG
                     NFSRRALALYERLRRPRAAELQRLGDEMAFFWNAGHPLVTGIRDRVFATLDRKDDLRH
                     KMLRLVAGLSDQRFTLWDRVRALGWP"
     gene            3038..3754
                     /locus_tag="AC16BC_02290"
     CDS             3038..3754
                     /locus_tag="AC16BC_02290"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR3240474.1"
                     /translation="MAVTPLRTLVIVPTYNERENVDALVAAALSALPDGDILVVDDAS
                     PDGTGESADALAARSARVHVLHRLRKEGLGPAYHAGFRWAVERGYDHVVEMDCDFSHD
                     PADLPRLVAAVRHGADLAIGSRYIPGARIEGWPWHRHALSAAANLYARAMLGRAVRDW
                     TSGFKCFRRAAVQAVLDAGAPANGYIFQVQGTYRVLRRGFSVVELPIAFRERTRGDSK
                     VRYNSAREAVLAVWTLRRNG"
     gene            3730..>3916
                     /locus_tag="AC16BC_02295"
     CDS             3730..>3916
                     /locus_tag="AC16BC_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240475.1"
                     /translation="MDASPKRIGDRPVAAWAAPAAVGLATLAAFVPVLSNAFIDWDDG
                     ANFLDNPSYRGLGWSYLT"
BASE COUNT          570 a         1387 c         1335 g          624 t
ORIGIN      
        1 gaggtgggac aagccggtct tgcccgtgat ctcgtcgcgg gaaccgacgc ggtaccagac
       61 ttgcacggag acgaccgggg ctttggcttc cgggatgaga atgacccgca gcccgttcgg
      121 aagcaccgac tcaatcgggg gagaggccca ggatgcgggt gcggacaggg ccaggcacag
      181 cacgatcgcg cccatccggg cgaatcgcaa catggcggga tgctaacgga acccggaaga
      241 tcgtgtcaag acgccgcggc ccgcgggaat aaacgcatcc agtgccggtc tttctcctta
      301 caggacggcc cgccccggtg ccctcggcat ccagggggtc cgaggcagtc gccgcgtcgg
      361 cggacgtccg ggaggggtgc catgcagtgc tcgcgctgtc gagggttgat ggtggtggac
      421 cgcttcacgg acttgttgga cgagaccggg cagattacgt tcgggggctg gcgatgtctg
      481 gcctgcggcg agatcgtgga cccggtgatc atgtccaacc gtcagcgcaa agtcgcgctc
      541 cccacgtcca aaccgcgaca cacacgctac ttgcagtgga tcggccggcc gcccagcgcc
      601 tgacccacgg gctgctgaaa aagtccatct gctgcgttct cggtcgtcgg aactcctcac
      661 gtacgacccc gtacgctccg ggttcctcct ccctgcggcc ttgcagctgg agctttttga
      721 gcagcccgta gcgccatgat tgtttcagcg ccctgccaac gcgtcgcaca tcgttgatct
      781 caaccggtat ccgaagtccg gatactggag gacgctcaga aatgcgtcag ctacaaggcg
      841 ccgcgagcac cggagcgagc gtacggggta ggtacgtgag caccggatgc gcagcggcaa
      901 cgcagcagct gaccatttat gagcgacctc ttacagcagg cgggtgaggg cgtccagcgc
      961 cgacaacatc tcgccgtccg cctccttgct ccgccggtac ggcatcccgc gcacgtccga
     1021 tccgccgacc cgccccttgc gcagatacgc ctcggccagg tagtgcagcc gttcgctgat
     1081 ccgcgccgcg tccgccgtcc gcgtcacttc ctgttcggac aaccccaggt cgcgtccgat
     1141 cgcctcggga ggttcgcccg ccgtgagccg acgcaagatt tcccgattgc gtcgggcgcc
     1201 ggccagcacc cgcgagacct ccaccaacac cagcccctcg gccacccgca ccgcgtacgt
     1261 cttggccagg atcgtggggt ccttgtcgtt ggctccggtc cggatgtcga acttccactc
     1321 gtgccacgga caggtaatgg tcatcccgca gatctcgccc tcgccgatgg gaccgccctg
     1381 gtgggggcaa ctgtgctcga gcgcgaagat ttcgccgccc acgttcagca aggcgatggg
     1441 tgtgccggcg accgtgacga ccttgacgcc gcccttcggg acctccgccg cttcagccac
     1501 tggaagatat tcaaccgcca cggatgcgtc ctttgcgtcg accaatggat gtgactcggc
     1561 gcgcgccgac cgcgggattc tacgcatccg cggcgcggcc tgtcaaaacg cccagcgcgc
     1621 cacgtagtgg cgcgccatgt caatgcgccg caggccactc ctggcgtggc gcagcaacgc
     1681 ccagcgcgcc acgtcaatac gccggaggcc acccctcgcc tggcgcagca acgggggcgt
     1741 gattgcattc atccggcgac gcctctacaa tcgagcctcg cacacgcccc gtgaccacgc
     1801 cccacgcgat acccgccgac ctgcaggccg accttgtgat cgtcggcgga ggcatgggcg
     1861 gcgccgtgct cgcgctgctc tccgcggcca agcggcgccg cgtggtggtg ctcgaccgcc
     1921 aaccgaccgg ccccaccgac cccaaccgcg gggaaatcgt ccagccgaac ggcctcgccg
     1981 tcctcgatcg gttgggactg ctcgccgggc tgcccgcgac cggggtctcc cgcgtgcgcc
     2041 ggtatcactt ctcccgcatt ggcggatcgc gactggtcac ggtggattac ggcgcgctgc
     2101 ccgcgccgta caactacacc ctggtgggcc atccccgcgc gctcatgacc acggtccggg
     2161 cgcgcctggc cgagtcgccg ttcatcgaga tgcggtgggg cgcgcaggtc gtcggcctgg
     2221 tgcgcgagcg ccgccgggtg gtcggggtgg aagcggacct cgacggcgtc cggacgcgcg
     2281 tcctggcgtc ggtcgtggtg ggcggcgacg ggtcggcctc gccggtgcgc gcggcgctcc
     2341 gaatccgcac ccgggtgcgc cggtacgcgg acggctatct caccgtcgtg ctgccccggc
     2401 ccgcggggtt cggcgaggac gggcggtatt acgtgggccg acgggaaatc ctgggggtgt
     2461 ttcctctggg cgacgatttc gtctacgcgt tctatctgtg gcccgcggga caacgcgagg
     2521 ccctggaggc ccgcgggctc cccgccttta cgagccggat cgcggcgatc gaccccgacc
     2581 tgcgcggcgc gctcgacgcg ctgacgcgat gggatcaggt cggctggatg ccgtgcgtca
     2641 aggtgacgcc ccgagcgtgg gtggccgacg gcgccgcgct gctgggcgac gccgcgcacg
     2701 cgctcaaccc ccacgtggcg cagggccgca accaggcgct cgaggacgcg gtggccctgg
     2761 acgcggtgtt ggactcctgc tttgccgcgg gcaacttcag ccggcgggcg ctcgcgctgt
     2821 atgagcgcct gcgacggccg cgcgccgcgg agctgcagcg cctcggcgat gagatggcgt
     2881 tcttctggaa cgcgggccac ccgctcgtga ccggcatccg cgaccgcgtg ttcgccacgc
     2941 tcgaccgcaa ggacgacctc cgccacaaga tgctgcgcct ggtggccgga ctgtccgacc
     3001 aacggttcac gctctgggat cgcgtccgcg cgctgggatg gccgtgacgc cgctccgaac
     3061 cctggtcatc gtccccacgt acaacgagcg ggagaacgtc gacgcgttgg tggcggccgc
     3121 gttgtccgcg ttgccggacg gggacattct ggtggtggac gacgcgtcgc ccgacggcac
     3181 cggcgaatcg gcggacgccc tggccgcccg ttccgcgcgc gtccacgtgc tccaccggct
     3241 ccgcaaggaa ggattgggcc cggcgtacca cgcgggattt cggtgggcgg tggaacgcgg
     3301 gtacgaccac gtcgtcgaga tggactgcga tttctcgcac gatcccgcgg acctgccgcg
     3361 actggtcgcc gccgtccgcc acggcgcgga tctcgcgatc ggctcgcgat acatccccgg
     3421 agcgcggatc gagggctggc cctggcatcg tcacgcgctg agcgccgcgg ccaatctgta
     3481 cgcgcgcgcg atgctcggcc gcgcggtccg cgactggacc agcggcttca agtgtttccg
     3541 ccgggccgcg gttcaagccg tgctggacgc gggcgcgccc gcgaacggct acatcttcca
     3601 ggtccaaggc acgtaccgcg tcctgcgtcg cggattctcg gtcgtcgagc tccccatcgc
     3661 gttccgggag cggacccggg gcgactccaa ggtccgttat aacagcgcca gagaagctgt
     3721 cctcgcggtg tggacgcttc gccgaaacgg ataggcgacc ggcccgtcgc ggcctgggcg
     3781 gcaccggccg cggtcggact cgccaccttg gcggcgttcg tcccggtcct gtccaacgcg
     3841 ttcatcgatt gggacgacgg cgcgaatttt ctcgacaacc cgtcgtatcg cggcctcggg
     3901 tggtcctacc tgacct
//
LOCUS       DBNGTT010000099         3250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2065061, whole genome shotgun sequence.
ACCESSION   DBNGTT010000099 DBNGTT010000000
VERSION     DBNGTT010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2065061"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="AC16BC_02300"
     CDS             complement(<1..254)
                     /locus_tag="AC16BC_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240476.1"
                     /translation="MSDLLSTLCPMFKEEVYRRTSRISNITMFGATALVAGMLWFATW
                     ADEFGGLERVLCAVGVIVFVGALLHQVSPDATRHPGAQDA"
     gene            468..>3250
                     /locus_tag="AC16BC_02305"
     CDS             468..>3250
                     /locus_tag="AC16BC_02305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3240477.1"
                     /translation="MPLSAFHPVINDWFQQRFGEPTEAQRLGWPHIVAQRDTLIAAPT
                     GSGKTLAAFLAAIDGLLRLAIDDRLIDETRVLYVSPLKALSHDVERNLTGPLEAIIER
                     AAATADRRLTPIRVAVRTGDTPAAERQRMTRRPPHIVVTTPESLYLLLTSVKGRAMLR
                     TVETVIVDEIHAVARDKRGSHLALSLARLDALCARRPVRVGLSATQAPIQEIARFLVG
                     TDRVKLDRTPDCAIVDVGHRRELDLAIDVPPSDLSAVCSNEQWEEVYARVAELIAAHR
                     STIVFVNTRRLAERITLRLTERLGEGAVLSHHGSLSKKIRRNTELRLKSGDLKAVVAT
                     ASLELGIDVGFIDLVVQIGSPRSIAVFLQRVGRSGHAVGHVPRGRLFPLTRDELMECA
                     ALIRSVRRGELDRVVIPEAPLDILAQQIVAETACEEWQEDDLYQRFRSAWPYRNLSRD
                     RFDRVVTMLAEGYATRVGRAGAYLHHDRIGKRIKARRAARLTALTSGGAIPELADYRV
                     VAEPDQTVVGSVDEDFAVESMSGDIFLLGNTSWQIRYVRAGDVVVHDAHGAPPTIPFW
                     RGEAPSRTAELSIALSDLRRELATRVTDVDAAALWLTTECLVKESAAREMVSYIQTQL
                     AAVGLLPTRDHLLIERFFDESGGMQLVIHSPYGGRINRAWGLALRKRFCRTFDFELQA
                     SADDDGIVLSLGPQQSFPLSDVPVMLPSSQAEHILTQAVLGSPIFATRWRWNANRALA
                     VPRQQHGRRVPPPLQRMRSDDLMVAVFPQQMACPENLTGPDIEIPDHPLVWQTMHDCL
                     HEACDLDGLSDLLRGIESGAIAITTIDTREPSPFAYEILNANPYAFLDDAPLEERRAR
                     AVATRRTLTVESVRDLGTLDPEAIERVRREAWPLVRDADELYDALMLIGLVSSDEARA
                     WGSQFEELVRAKRA"
BASE COUNT          552 a         1102 c         1048 g          548 t
ORIGIN      
        1 aacgcatcct gggctcccgg gtggcgcgtg gcatcgggcg agacttggtg cagcaacgcg
       61 cccacgaaga cgatcacacc caccgcgcac aacacgcgtt ccaagccccc gaactcatcg
      121 gcccaggtcg cgaaccacaa catcccggca accaacgcgg tggccccgaa catcgtgata
      181 ttgctgatcc gggacgttcg acggtagacc tcttccttga acatcgggca caacgtggac
      241 aatagatcgc tcatgcatcc tccggaataa ccgtcgtggg gtagagccca atcctaggcg
      301 cttgagggat cgagtgtcaa ccgcttactc ggaagatcag aagtttggta acaggtgtat
      361 ccattcgccc cccgtccccc ctttgaaaaa ggggggacgg ggggatttgc cgtgctcgcg
      421 ttcccccgtg cctttcgcct atcggagtcg gtatgataca gactgccatg ccgctctccg
      481 cctttcaccc ggtgatcaac gactggtttc aacagcggtt cggcgagccc accgaggctc
      541 agcgccttgg ctggccgcac atcgtcgcac aacgcgatac gctgatcgcc gcgccgacag
      601 gttccgggaa aacgcttgcc gcgttcctgg ccgccatcga cggacttttg cggcttgcaa
      661 tcgacgaccg gctgattgac gagacccgcg tgctctacgt gtctccgctg aaagcgctca
      721 gccacgacgt ggaacgaaac ctgaccggcc cgttggaagc gatcatcgaa cgcgcggccg
      781 cgacggccga ccgtcgcctc acgcccatcc gcgtggcggt ccgcaccggg gacacgccgg
      841 ccgcagaacg gcagcgcatg acccgtcgcc cgccgcacat cgtggtcacc accccggagt
      901 cgctctatct cctgctgacg tcggtcaaag gccgcgccat gctgcggacc gtggagaccg
      961 tcatcgtgga cgagatccac gcggtggcgc gggacaaacg cggctcgcac ctggcgctgt
     1021 cgctggcccg gctcgatgcc ttgtgcgcgc ggcggcccgt gcgcgtgggg ctgtcagcaa
     1081 cgcaggcgcc gatccaggag atcgcgcggt tcctggtcgg aacggatcgc gtgaaactcg
     1141 accgcacgcc ggattgcgcg atcgttgacg tggggcaccg gcgcgagttg gatctggcga
     1201 tcgacgtgcc cccgtccgac ctttccgcgg tctgttccaa cgaacagtgg gaggaggtct
     1261 acgcccgggt cgcggaattg atcgcggcac accgcagcac catcgtgttc gtgaatacgc
     1321 gtcgtcttgc tgaacgcatc accttgcggc tcaccgagcg tctgggtgaa ggcgccgtgc
     1381 tctcgcacca cggcagcctg tccaagaaga tccggcggaa cacggagctg cgcctcaaat
     1441 ccggcgatct caaggccgtg gtggccaccg cgtcgctcga actcggcatc gacgtggggt
     1501 tcatcgacct ggtggtgcag atcggatcgc cccgttccat cgccgtgttc ctccagcgcg
     1561 tgggccggtc cggccacgcc gtgggccacg tcccgcgggg acgtctgttc ccgttgacgc
     1621 gggacgagtt gatggagtgc gcggcgttga tccggtcggt gcgtcgcggc gagctcgatc
     1681 gcgtggtgat cccggaggcg ccgctcgaca tcctcgcgca gcagatcgtg gcggaaaccg
     1741 cgtgcgagga atggcaggag gacgacctgt atcaacgatt ccgctcggcc tggccgtatc
     1801 ggaacctgtc ccgcgatcgc ttcgaccggg tcgtaaccat gctcgcagaa ggctacgcca
     1861 cccgcgtggg ccgagcgggc gcgtacctgc accacgaccg catcggcaaa cgcatcaagg
     1921 cccgccgcgc cgcgagactc accgcgctca cctcaggcgg cgcaatcccg gagctggccg
     1981 actaccgcgt ggtggcggag ccggatcaaa ccgtggtggg atcggtcgac gaagacttcg
     2041 cggtcgaaag catgtcgggc gacatctttc tgctcgggaa cacgtcgtgg cagatccggt
     2101 acgtgcgcgc gggcgacgtg gtggtgcacg acgcgcacgg cgcgccgccc accatcccgt
     2161 tctggcgcgg ggaagcgccg tcgcgcaccg ccgaattgtc catcgcgttg tcggaccttc
     2221 ggcgcgaact ggcgactcga gtgaccgatg tggatgcagc ggctctgtgg ctgaccaccg
     2281 aatgcctggt caaggagtcc gcggcgcggg aaatggtctc gtacatccag acgcaacttg
     2341 cggccgtcgg actcctgccc acgcgcgacc acctgctgat cgaacgcttc ttcgacgagt
     2401 ccggcgggat gcagctcgtg atccattcgc catacggcgg acgcatcaac cgcgcgtggg
     2461 gcctggccct gcgcaagcgc ttttgccgaa cgttcgactt cgaactgcaa gccagcgcgg
     2521 acgatgacgg catcgtcttg tcattgggcc cgcagcagag tttcccgttg tcggacgtcc
     2581 cggtcatgtt gccgtcgtcc caggccgagc atatcctgac ccaggccgtg ctgggctccc
     2641 cgatctttgc cacgcgctgg cgctggaacg cgaaccgcgc gctcgcggtc ccgcgccagc
     2701 agcacggccg ccgcgtgccg ccgccgctcc agcgcatgcg ttctgatgac ttgatggtgg
     2761 cggtgttccc ccaacaaatg gcgtgccctg agaatctcac cggcccggac atcgagatcc
     2821 ccgaccaccc gctcgtgtgg cagaccatgc acgactgttt acacgaagcg tgcgatctcg
     2881 acggcttgag cgatttgctg cgcgggatcg agtccggcgc gatcgcgatc accacgatcg
     2941 acacgcgaga gccttccccg ttcgcgtacg agatcctcaa cgccaacccc tatgcgttcc
     3001 tggacgacgc gccgctggag gagcgccgcg cccgcgccgt ggccacgcgc cgcacgctca
     3061 ccgtggaatc ggtccgcgac ctgggcacct tggaccccga agccatcgaa cgcgtccggc
     3121 gcgaagcctg gccattggtt cgcgacgcgg acgagctgta cgacgcgctg atgctgatcg
     3181 ggctagtgtc gagtgatgaa gcacgggcgt ggggttcaca gtttgaggaa ctggtacgag
     3241 caaagcgcgc
//
LOCUS       DBNGTT010000100         3485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_659876, whole genome shotgun sequence.
ACCESSION   DBNGTT010000100 DBNGTT010000000
VERSION     DBNGTT010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_659876"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="AC16BC_02310"
     CDS             <1..582
                     /locus_tag="AC16BC_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3240478.1"
                     /translation="HRAGGLPSRPLIRRLAALFALDSFAGGLVIQSLVVYWFHTHFGL
                     GLASLGWIAFGTQVLSGLSLLLAPPLARRFGLVPTMVFSHLVSNCFLIALALAPTVAV
                     AVSLLLARHLLSQIDVPTRQTFLMLAVEDHERETAAVVTNTSRTLVQAVSPALTGWVM
                     QVISLSAPFIVGGGLKIAYDIMLYAAVRNVRHK"
     gene            complement(931..1006)
                     /locus_tag="AC16BC_02315"
     tRNA            complement(931..1006)
                     /locus_tag="AC16BC_02315"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(971..973),aa:Phe,seq:gaa)
     gene            complement(1084..3027)
                     /gene="metG"
                     /locus_tag="AC16BC_02320"
     CDS             complement(1084..3027)
                     /gene="metG"
                     /locus_tag="AC16BC_02320"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR3240479.1"
                     /translation="MTTQQPPRIDSRHTYYITTPIYYVNDVPHIGHAYTTVAADVLAR
                     YHRLLGDDVFFLTGTDEHGQKVHQAATKRGVPPQQHVDELVGRFQDLWKRLDISNDDF
                     VRTTQARHTAVVQGVLIRLRDRGELYQQTYQGWYCLPDERFWADDEVVDGLCPECRRP
                     VERLAETNYFFRMSKYHRRLLQHIETHPEFIRPESRRNEVLGFLAQSLNDLCISRPKA
                     RLPWGVPFPFDDAFVTYVWVDALVNYISIPGYASDDARFARYWPANVHLVGKDILTTH
                     AVYWSTLLMALDLPLPKTIFAHGWWTVDGEKMSKSRGNVVDPGAVVNEFGVDPFRYFV
                     LREVPFGQDGDFSRDAFIGRYNSDLANDLGNLVSRTLTMIERYAGGKIPAQGPDDGFE
                     EAVAAAVREVHNSLENLQFHRALQELWMLVDRANRYIESTAPWSLAKDPAQRVRLDDV
                     LYVLAETLRHVARLISPFMPGTSRAIADSLGLSKVPNGGPATWGYLPKDQQISKPPAL
                     FPRRTSVSDTPPVAAPAAGQPVQAAAPPAPQTTQISIDDFAKVELRVGKILAAERVPK
                     SSKLLKLQVDLGTETRQIVAGIGTKYEPDALIGRLVAVVANLKPAKLMGIESQGMILA
                     AGDKEVTALLAYVEPVEPGMRIK"
     gene            complement(3176..>3485)
                     /gene="ricT"
                     /locus_tag="AC16BC_02325"
     CDS             complement(3176..>3485)
                     /gene="ricT"
                     /locus_tag="AC16BC_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HIR3240480.1"
                     /translation="VDFRALVKDLAGKFRARIEMKQIGVRDEAGLLGGIGSCGLQLCC
                     SLFLTKFHPVTTKMVKAQNLPVNNSQLLGSCGRLKCCLAYEYYGPEPKQPKLVQIQKL
                     "
BASE COUNT          609 a         1038 c         1138 g          700 t
ORIGIN      
        1 caccgcgccg ggggtctgcc gtcacggccc ctcattcggc ggctggcagc cctctttgcg
       61 ctcgattcgt ttgccggtgg actcgtcatt caaagcttgg tcgtgtactg gttccataca
      121 cacttcggtc tcgggttggc gtcgctcggc tggattgcct tcgggactca ggttctgtcc
      181 ggcttgtcgc tgctgttggc cccgccgctg gcgcggcgtt ttgggcttgt gccgacgatg
      241 gtgttttctc atctcgtgtc caattgtttc ctgattgcac tggcgctggc tcctaccgtc
      301 gccgtggccg tctcgttgct gctggcgcgg catctgctct cgcagattga tgtgcccacg
      361 cgccagacgt ttctgatgct cgccgtggaa gaccacgagc gggaaaccgc agccgttgtc
      421 accaacacga gccggaccct ggtgcaggcc gtcagtccgg ccctgactgg ctgggtgatg
      481 caggtgatct cgctctccgc gccgttcatc gtaggaggtg ggctcaagat cgcctacgac
      541 atcatgctct acgccgcggt ccggaacgtt cgccacaagt gaggcacgtt ggagttggaa
      601 ttgtcgcagc gtattgcaga ggaggttggc aagaggcttg tgggagggtg agacagacac
      661 tcgcaccacc acgtctgcga taggcagagc cgtctggtgg cgcaataaat atgggtttcc
      721 aaggcgaaaa accggacact cggacacaag gtctcttaca cccgagagca actcctaaat
      781 gggatattca gatcctcccg aggggttttt cggggacctt ttggtctctt gatcaccgaa
      841 atttacggaa agtggtagca aaccggtggc agggagagtg agggtaaaaa cagatggctt
      901 cgcgaacccg ttgaaaaccg aaaagaaaag tggtgccgag ggggagaatc gaactcccga
      961 cacaaggctt ttcaggcctc tgctctaccg actgagctac ctcggcgcca tgcggcgttc
     1021 aggcgggaaa aatcaaaggt cgtaccatag caaacgcgtt gttgcgaagt caacgcgcgg
     1081 gcgttatttg attcgcattc ccggttccac gggttcgacg taggcgagga gggcggtgac
     1141 ttccttatcg ccggccgcga ggatcatgcc ctgggattcg atgcccatga gtttggcggg
     1201 cttgagattg gccacgaccg cgactaaccg gccgatcaac gcatcgggtt cgtacttggt
     1261 tccgatgccg gccacgatct ggcgggtttc ggttcccaga tccacttgca gcttgagcag
     1321 cttggaggac ttgggcacgc gttccgcggc gaggatcttg cccacgcgca attcgacctt
     1381 ggcgaagtcg tcgatgctga tctgagtggt ctgcggagcg ggcggcgcag cggcttggac
     1441 aggctgaccg gccgcgggtg cggcaacagg cggtgtgtcg gacacggacg ttctccgggg
     1501 aaacaatgcg ggtggtttcg agatctgttg gtccttggga agataccccc acgtggcggg
     1561 cccgccgttc gggaccttcg acagccccag gctgtccgca atggcgcgcg acgtcccggg
     1621 catgaagggg gagatcaagc gcgcgacgtg gcgcaatgtt tccgccagca cgtagagcac
     1681 gtcgtcgagc cgcacgcgtt gcgccgggtc cttggccagg ctccacgggg ccgtggactc
     1741 gatgtagcgg ttggcgcgat ccacgagcat ccacagctcc tgcaacgcgc gatggaactg
     1801 aaggttctcc agcgaattgt ggacctcgcg gacggccgcg gccacggcct cttcgaaccc
     1861 gtcgtccggg ccctgcgccg gaatcttgcc gccggcgtag cgttcgatca tggtgagggt
     1921 ccggctcacc aggttgccga ggtcattggc caggtcgctg ttgtagcggc cgatgaacgc
     1981 gtcgcgcgaa aagtcgccgt cctgtccgaa cggcacttcg cgcagcacga aatagcggaa
     2041 cgggtccacg ccgaactcgt tgaccaccgc gcccgggtcc accacgttgc cgcggctctt
     2101 ggacatcttc tcgccgtcca cggtccacca cccgtgggcg aagatcgtct tgggcaacgg
     2161 caggtcgagc gccatgagca gggtggacca gtagaccgcg tgcgtggtca ggatgtcctt
     2221 gcccaccagg tggacgttcg cgggccagta gcgtgcaaac cgcgcgtcat cggacgcgta
     2281 ccccgggatc gaaatgtaat tgaccagcgc gtccacccac acgtaggtga cgaacgcgtc
     2341 gtcgaacgga aacggcacgc cccatggcag ccgcgccttc ggacgggaga tgcacaggtc
     2401 gttgagcgac tgcgccagga atcccagcac ctcgttgcgc cgactttccg ggcggatgaa
     2461 ctcgggatgg gtctcgatgt gctggagcag ccgccggtgg tacttgctca tccggaagaa
     2521 atagttggtc tcggcaaggc gctccaccgg gcgccggcac tccggacaca acccgtcgac
     2581 cacttcgtcg tcggcccaga agcgttcgtc gggcaggcag taccagcctt gatacgtctg
     2641 ctggtacagt tccccccggt cgcgaagcct gatcaacacc ccctggacca ccgcggtgtg
     2701 gcgcgcctgc gtggtgcgca cgaagtcgtc gttggagata tcgagtcgct tccacaggtc
     2761 ctgaaaccgg cccaccagtt cgtccacgtg ttgctgcggg ggcacgccgc gtttggttgc
     2821 ggcctggtgc actttctgac cgtgttcgtc cgtcccggtc agaaaaaaca cgtcgtcgcc
     2881 gagcaaacgg tgataccgcg ccaacacgtc ggccgcgaca gtggtgtagg cgtggccgat
     2941 gtgcgggacg tcgttgacgt agtagatggg ggtggtgatg tagtaggtat gccggctgtc
     3001 gatacggggc ggctgctgcg ttgtcacgtc agcctcgcat tgcttcgcca cccacaagtc
     3061 gggttctcac gtacgaacca gtacgctccg gtgctcggct tcgctccgcc ttgcatacgc
     3121 cgccttctcg acagccggtt gggacgagtc ggtgtcttgg actgggacag aaatttcata
     3181 atttctgaat ctgtaccagc ttgggctgtt tgggttccgg gccgtagtac tcgtaggcca
     3241 ggcagcactt gaggcggccg cacgagccga ggagctggga gttattgacc gggaggttct
     3301 gcgccttgac catcttggtg gtcacggggt ggaacttggt caggaagagc gaacagcaca
     3361 gttgcagccc gcaggagccg atgccgccga gcaggcccgc ttcgtcgcgc acgccgatct
     3421 gtttcatttc gattcgcgcg cggaacttgc cggccaggtc tttgaccagc gcgcgaaaat
     3481 ccacg
//
LOCUS       DBNGTT010000101         2804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1809413, whole genome shotgun sequence.
ACCESSION   DBNGTT010000101 DBNGTT010000000
VERSION     DBNGTT010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1809413"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="AC16BC_02330"
     CDS             <1..634
                     /locus_tag="AC16BC_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240481.1"
                     /translation="GLVYKRQDKLDPAEMEFTAVLTANPEMAAAFFELGQVYARMGKT
                     GAAAAAYREVAAINPYVGNVNTRLGEMALADNKLDSAIVYLKKALERDTEPFMIYFLL
                     ANAYQKRGNLPEAAAAYQHVIKLFPVLFDARYNLGIVYGKQHRWSDAATEFEETLKLN
                     ARFSPAHYQLATAQTKLGKRKEAKQHLQEYLKNAPDGEYVADAKKLLASL"
     gene            638..1357
                     /locus_tag="AC16BC_02335"
     CDS             638..1357
                     /locus_tag="AC16BC_02335"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR3240482.1"
                     /translation="MTRQKPVRPDAAAARAFQRRLLSWYRRHGRDLPWRKTSDPYEIL
                     VSEIMLQQTQVERVIPKYHEFLGKYPTLEELAAARVEEVTETWYPLGYNIRPVRLHGI
                     AREAVARYGGEIPSSEEDLLSMKGIGRYTAGAVRSFAFGQDAPILDTNVARVLSRVFL
                     PKADPKRGADKTKLWQLSEALIPSGKGYDFNQALMDFGATVCTARKPACPTCTMRPIC
                     KSYQRPARPRVSRKPRTRSRG"
     gene            1320..1730
                     /locus_tag="AC16BC_02340"
     CDS             1320..1730
                     /locus_tag="AC16BC_02340"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIR3240483.1"
                     /translation="MSPASPGPAPAVEIAAAVIGNHGRYLIARRPDHVVLGGYWEFPG
                     GKRRVDESFEDCVRREVREEVGVEVAVGDLIERVVHRYPHGTVDITFLNATLVRGEPV
                     ARGCAEIRWVTPEELPAYRFPPANDTVIRRLTGR"
     gene            1778..>2804
                     /gene="thiI"
                     /locus_tag="AC16BC_02345"
     CDS             1778..>2804
                     /gene="thiI"
                     /locus_tag="AC16BC_02345"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR3240484.1"
                     /translation="MRYALVHYHELILKKGNRAYFVRRLIRNLQKGLKGTGVSEITQE
                     GARLLIGLHDRADEGEIRARLARTFGVANFAIARWLPLDTAVLSQAVAEAVKARSFAS
                     FRISAKRSNKAFPLTSNQINVELGALVQRITGARVDLDHPEFTVYAEVIDRGILLSLD
                     RQSGPGGLPAGVSGRVACLMSGGLDSPVAAYRMMKRGAYVVFVHFHSHPFVSRASQEK
                     AKELAELLTKYQYASRLFLVPFGEVQREVVLAVPGPLRVIVYRRLMIRIAEAIARTVR
                     AQALVTGEALGQVASQTLSNMTTVDAVATIPVLRPLVGMDKEEIVHQARALGTYEISI
                     QPDEDCCT"
BASE COUNT          512 a          917 c          896 g          479 t
ORIGIN      
        1 gggcctggtg tacaagcgac aagacaaact cgaccccgcg gaaatggagt ttacggccgt
       61 gttgaccgcg aatccggaga tggccgccgc gtttttcgaa ttgggacagg tgtacgcgcg
      121 aatgggaaag accggcgcgg cggcggccgc ctaccgcgag gtggccgcaa tcaatccgta
      181 cgtgggcaac gtcaacaccc gtctgggaga aatggcgttg gccgacaaca agctcgactc
      241 cgcgatcgta tatctcaaaa aggcgctcga acgggacacc gaaccgttca tgatttactt
      301 cctactcgcc aacgcttacc agaaacgcgg gaacctcccg gaagcggccg ccgcgtatca
      361 gcacgtgatc aaattgttcc cggtgctgtt tgacgctcgc tacaacctcg gcatcgtgta
      421 cggcaagcaa catcgctggt cggatgcggc cacagaattc gaagaaacct tgaaactcaa
      481 cgcgcgattc agcccggccc actatcagtt ggccacggcg cagaccaagc tcggtaagcg
      541 gaaggaagcc aaacaacacc ttcaggagta tctcaagaac gcgccggacg gtgagtacgt
      601 cgccgacgcc aagaaactcc tcgcgtcgct gtaacggatg acgcgccaga aaccggtacg
      661 cccggatgcc gccgcggcgc gcgcgttcca gcgacgcctg ttgtcctggt atcgccgcca
      721 cgggcgggat ctcccgtggc gcaagacctc cgacccctac gaaattcttg tctcggagat
      781 catgctgcag cagacccagg tcgagcgcgt gattccgaag tatcacgagt tcttggggaa
      841 atacccgacg ctcgaagagt tggccgccgc gcgggtcgaa gaggtcacgg aaacctggta
      901 tcccctcggc tacaacatca ggcccgtccg cctgcacggc atcgcccgcg aggccgtggc
      961 gcgctacggc ggtgagatcc cgtcgtccga ggaagacctg ctgtcgatga agggcatcgg
     1021 ccgctacacc gcgggcgcgg tgcgcagttt cgcgttcggc caggacgccc cgattctcga
     1081 caccaacgtg gcgcgggtgc tctcgcgggt gtttcttccc aaggccgatc ccaagcgcgg
     1141 ggcggacaag accaagctgt ggcagctctc cgaagcgttg atcccgtccg gcaaggggta
     1201 cgacttcaat caggccttga tggatttcgg cgccaccgtg tgcaccgcgc gaaaacccgc
     1261 gtgcccgacc tgcaccatgc ggccgatttg taagagctac caacgcccgg cgcgcccgcg
     1321 tgtctcccgc aagccccgga cccgctcccg cggttgaaat cgccgcggcc gtaatcggga
     1381 accacggccg ctacctcatc gcccgacgcc ccgaccacgt ggtgctgggc gggtactggg
     1441 agttccccgg cggcaaacgc cgcgtcgacg aatcgttcga ggactgcgtg cgtcgcgagg
     1501 tgcgcgagga agtgggcgtc gaggtcgcgg tcggcgacct gatcgagcgc gtcgttcacc
     1561 ggtaccccca cggcaccgtg gatatcacct ttctgaacgc caccctggtg cgcggagaac
     1621 ccgttgcgcg cggctgcgcc gagattcggt gggtgacgcc ggaggaactc ccggcgtacc
     1681 ggttccctcc ggccaacgac accgtgattc gccggctgac cggacgctga ccgtcggctc
     1741 gcttgcaacc cgccccacgc ttcggtatcg taccggcgtg cgctacgcat tggtccacta
     1801 tcacgaattg atcctgaaga agggcaaccg cgcgtatttt gtccgccggt tgatcagaaa
     1861 ccttcagaag ggcctcaagg gcacgggcgt cagcgaaatc acccaggagg gtgcccgatt
     1921 gctgatcggc ctgcacgacc gcgcggacga aggagagatc cgtgcccggc tggctcgcac
     1981 cttcggggtc gccaatttcg ccattgcgcg gtggctcccc ctggacacgg ccgtgctgag
     2041 ccaagcggtc gccgaggcgg tgaaggcgcg ctccttcgcc tcgttccgta tctccgccaa
     2101 gcgaagcaac aaggcgtttc cgctcacatc caatcagatc aatgtcgagc tcggcgcgct
     2161 tgtccagcga atcaccggcg cgcgcgtgga tctcgaccac cccgagttca cggtgtacgc
     2221 ggaagtgatt gaccgcggca tcctgctctc gctcgatcgc caatcgggac ccggggggct
     2281 gccggccggc gtgtcgggcc gggtcgcgtg tctgatgtcc ggcggtctcg actccccggt
     2341 ggccgcgtat cgcatgatga aacgcggggc gtacgtggtg ttcgtgcact tccacagcca
     2401 tccattcgtg tcccgcgcct cgcaggaaaa ggccaaggag ttggccgagt tgctcacgaa
     2461 gtaccagtac gcctcgcggt tgttcctggt cccgttcggc gaggtgcagc gcgaggtcgt
     2521 gctggccgtt ccgggaccct tgcgcgtgat cgtgtatcgt cgactcatga ttcggatcgc
     2581 cgaggccatt gcccgcacgg tgcgcgcgca ggcgttggtc acgggcgagg ccttggggca
     2641 ggtggcgtcg cagaccttgt ccaacatgac caccgtggac gccgtggcca ccataccggt
     2701 gctcaggccc ctcgtgggta tggacaagga ggagatcgtg caccaggcgc gggcgctggg
     2761 cacgtacgag atttcgatcc agcccgacga ggattgctgc acct
//
LOCUS       DBNGTT010000102         4159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1298531, whole genome shotgun sequence.
ACCESSION   DBNGTT010000102 DBNGTT010000000
VERSION     DBNGTT010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1298531"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="AC16BC_02350"
     CDS             <1..260
                     /locus_tag="AC16BC_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240485.1"
                     /translation="TATTLSFVDISGNAVTYTSGGGSVTRNGAMLASSGSTTLTFSYL
                     QQNGTVAAAAAQVWVIQMAFTFGVGSETQSFRVRVHPRNFT"
     gene            257..1156
                     /locus_tag="AC16BC_02355"
     CDS             257..1156
                     /locus_tag="AC16BC_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240486.1"
                     /translation="MITARINRERGMSLIAVIFFVVIMGFLGVTLASLMSTQASTSAG
                     ELSSTQALYVAEGGGEFAQRALAQNLDWYRSAADPIVTPATVLGPGSFTGNIFLPATE
                     LSRRVTPASGTIRVFSTARFPASGILQLGHDIGSGEFVQYTVADATTFNVVARDVTVG
                     TVNGGAAGTYARGTAVFPVTMLNALLSAVSATCSPTPTAAAITLAANTKFLPTGTITV
                     DSEEITYTGSSTSGGIATLTGVTRCVNSQSSGASHPALYPVTPLLDDNAAPDFEVFMM
                     STGSVGSAPLSVAARVVQKTVQR"
     gene            1203..2840
                     /locus_tag="AC16BC_02360"
     CDS             1203..2840
                     /locus_tag="AC16BC_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1470 family protein"
                     /protein_id="HIR3240487.1"
                     /translation="MILGTWNGQAEATRNFVATQTPAPNVPVSMGTTGTLTYRITNSN
                     TNPNTGERIYEVRFRMSGGRSTFSSSVAAPAGWTRTSYSTTSVTFRAASWANTISTGS
                     YLDFPIIFNFRQTTADTNELLRDIRARNTTSSGGPPFTNLGSDTDSNQGGWTLKSLSI
                     TSFQTTDLSGVPVSAVAYGAQFRLVMTIRNNSTTTQTAIVSNTNPPGRVAGSLTPTGP
                     SSTVYSPNPLNLAPGASGTITFTYTAPSGSPGTIQFTAAARNGANTATSPIVLSNILV
                     VSPLNASIAITPTCLFSGDTATFRMTVTNNTGSAVSNVTPSALTRVTYDSAAIGAFSA
                     SSPASFNLNAGASNTFTWTATVTGTVLAAGPKPSFEVTGSASANGGLVTPVATSNRED
                     IDGYVVSVAPSDTNASSTNQELTWTVANRGCAAVNSVSILIDPDWTYANDAYSAVDVS
                     AVNTVETWSAGTPNPLQFTAAPTAAERLPIDFTGDFSLVFSTLPVTPETSNFQVTITD
                     AAVPPNTRIIPMSVNVLAFDPTGPNAAGTLIWQEQVR"
     gene            2950..3219
                     /locus_tag="AC16BC_02365"
     CDS             2950..3219
                     /locus_tag="AC16BC_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3240488.1"
                     /translation="MKTNDLLKQIDIPRHKLYYLEQKGYVTPTRIPMGDLEAREYSTA
                     DVELVAAIWKYLKKGFKHRVAYEKALDDIGRVPPVARRVRRREAA"
     gene            3231..>4159
                     /gene="pilM"
                     /locus_tag="AC16BC_02370"
     CDS             3231..>4159
                     /gene="pilM"
                     /locus_tag="AC16BC_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIR3240489.1"
                     /translation="MRRSRVGCDFGHSAVKLVQLKRQGQSWSVVRAELRELTPEACTG
                     DDWPSRQAVQVVSECQWAQGEDLAVSLQRRPALVRYLSLPEMPNRELREALQWEAKKA
                     ASQAVEELVVDYVSGPPVKGEQGRTMPVTMVVADRRAVEDEFAVYRETGLRVKAMDVN
                     PLSFYYAAHRLGRDQTGPGFVAFVDIGASRLDINIAKQGTLRFSRSVALGGDTLTENL
                     ARALGVDQAQAEGIKREEGLSGQAKVLDVLGPDVDRLVVEIQRSVDYYRAQAHDGALE
                     ALWIGGGTGLIPGFVAYVGKFFDANVRLFNPFEG"
BASE COUNT          847 a         1246 c         1236 g          830 t
ORIGIN      
        1 tgaccgcgac cacgctgagc ttcgtggaca tctcggggaa tgccgtcacc tacacatcag
       61 gcggcggttc ggtgacgcga aacggcgcaa tgctcgcctc ctccggcagc actacactga
      121 ccttttcgta cttgcaacaa aacggaacag tggcggccgc cgcggctcaa gtatgggtaa
      181 tccaaatggc attcacattc ggcgtcggga gtgagaccca gagctttcgc gtccgcgttc
      241 acccaaggaa ctttacgtga ttacggcgcg catcaacagg gagcgcggga tgtcgctcat
      301 cgcggtgatc ttcttcgtgg tcatcatggg gtttttgggc gtcacgctgg cgtccttgat
      361 gagcacgcag gcgagcacgt cggccggcga gttgagttcg acgcaagcgc tctatgtagc
      421 ggaagggggg ggcgagtttg cacagcgggc tctcgctcag aacctagatt ggtaccgcag
      481 tgcggcagat cccatcgtca cacctgccac agtactgggt cccggttcat tcaccggcaa
      541 catatttctc ccagcgaccg agctgtcgcg acgcgtcacg ccggcctcgg gaaccattcg
      601 agtcttctcc accgcgcgtt tcccggcgtc aggaattctc cagcttgggc acgatatcgg
      661 gagcggcgag ttcgtccagt acactgtcgc ggatgcgacc accttcaacg tcgtggctcg
      721 ggacgttacc gtcggtacgg tgaacggagg tgcggccggt acctatgcgc gaggaacggc
      781 cgtttttccc gtgacgatgc tgaatgccct cctctcggcc gtgagcgcga cgtgcagccc
      841 gacacctaca gcggcagcga tcacccttgc cgccaacacg aaattccttc cgaccggaac
      901 tatcactgtt gattcggaag agattacgta cacgggatcg agtacatcgg gggggattgc
      961 gacgctgacc ggcgtcacgc gttgcgtcaa ttcccaatcc agcggagcaa gtcacccagc
     1021 gttatatccc gtgactccgc tccttgacga taatgctgct ccagactttg aagtgttcat
     1081 gatgtccaca ggctcggttg gatccgctcc gctcagtgta gcggctcggg tcgtgcagaa
     1141 gaccgttcag cgttgagagg ggggatatgc gacgactggc catcacgatc gggaccgtaa
     1201 tgatgattct cgggacctgg aacggccaag ccgaagcaac gcgaaatttc gtcgccacgc
     1261 agaccccggc gcccaatgtg cctgtctcca tgggaaccac gggaacgctg acgtaccgca
     1321 tcaccaattc gaacaccaac cccaacaccg gtgagcgtat ttacgaggtt cgattccgca
     1381 tgtcgggcgg gcgaagcact ttttcatcat ccgttgccgc gccggccggg tggaccagaa
     1441 cctcgtatag cactacctcg gtgacgttcc gcgccgcttc ttgggccaac acgatttcaa
     1501 ccggatcgta tctggacttt ccgatcatct ttaatttccg acagaccacc gccgacacca
     1561 atgaactgct gcgcgacatc cgtgccagga acaccacgag ctccggcggg ccaccgttta
     1621 ccaacctcgg ctccgataca gacagcaacc agggtgggtg gacactcaag tcgctgtcca
     1681 tcacgtcttt ccaaacgacc gacctctccg gcgtccccgt gagtgccgtt gcctatgggg
     1741 cgcaattccg tctcgtgatg acgatccgaa acaactccac aacaactcaa acggcgattg
     1801 tctcgaacac gaatcctccc ggccgtgtgg cgggctccct cacgccgacc ggcccgtcat
     1861 ccacggtcta tagccctaac ccattgaact tggcaccggg ggcgtctggg accattacct
     1921 tcacttacac ggctccgtcg ggtagtccag ggacgattca atttaccgct gccgcgcgca
     1981 acggcgcaaa cacggccacg tcgccgatcg tcctttccaa tatcctggtc gtgagtccct
     2041 tgaacgccag catcgcgatc acgccaacgt gtctgttttc gggggatacc gcgaccttta
     2101 gaatgacggt gaccaataac accggtagtg cggtcagtaa cgtgacgcca tcagcgctga
     2161 cgcgagttac gtatgattct gccgccatcg gagcgtttag tgcatcaagt ccggcttctt
     2221 ttaatctcaa tgccggggca tcaaacacgt tcacctggac cgcaacagtg accggtactg
     2281 ttctggcggc cggccccaag ccatcgttcg aagtgacggg gtctgcgtcg gcaaacggcg
     2341 gcctcgtaac gccagtggcg acgtcgaatc gtgaggatat cgacggatac gtggtcagcg
     2401 tggcgccgtc tgacacgaat gcctcaagca cgaatcaaga actcacctgg accgtcgcca
     2461 accgagggtg cgcggcggtt aatagcgttt ctatcttgat cgacccagat tggacttacg
     2521 ccaatgatgc ctattcggcg gtggacgtca gcgcggtaaa cacggttgaa acctggagcg
     2581 cgggcacacc aaatcccctg caatttaccg cggcaccgac agccgcggaa cggcttccga
     2641 tcgactttac aggtgatttc agtttggtgt tctccacact gcccgttaca cctgaaacca
     2701 gtaactttca ggtcacgatc accgacgcag cggttcctcc caataccaga atcattccca
     2761 tgtcggtaaa cgttctagcc tttgatccaa cgggacccaa cgcggccggt acgcttattt
     2821 ggcaggaaca ggttcgctaa ccggcaattc atcgacaaga ttcgcctctg tcaatttctt
     2881 gacacgtttg gcatcacgac tagacttgcc tctcagtgta gaaaaataga tttgtcagtc
     2941 gagggaacgg tgaagaccaa cgacctgctc aagcaaatcg atatcccaag gcataagctc
     3001 tattacctcg aacaaaaggg gtacgtgact cccacaagaa ttccgatggg ggatctcgaa
     3061 gctcgggagt actcgactgc cgacgtggag ttggtggccg ccatctggaa gtacctcaag
     3121 aagggattca agcaccgagt tgcatacgag aaagcgctcg atgacatcgg ccgcgtgccg
     3181 ccggtcgcgc gccgcgttcg acgacgggaa gccgcgtaat gatgccgtgg atgcgtcgct
     3241 cgcgtgtggg gtgcgatttc gggcacagcg cggtcaagtt ggttcagttg aagcgtcagg
     3301 ggcagtcgtg gtccgttgtg cgcgcggaat tgcgtgaatt gacgcccgag gcgtgcaccg
     3361 gtgatgactg gccgagccgt caggcggtgc aggttgtctc cgagtgtcag tgggcgcagg
     3421 gcgaagacct cgcggtcagc ctgcaacgcc gacccgcgct ggtgcgttat ctgagccttc
     3481 cggagatgcc caaccgcgag ttgcgtgagg cgctgcaatg ggaagcgaaa aaggccgcgt
     3541 cccaagccgt ggaggaattg gtggtggatt acgtcagcgg acccccggtc aagggggagc
     3601 aagggcgcac gatgccggtg acgatggtcg tggcggaccg ccgggccgtc gaggacgaat
     3661 tcgccgtgta tcgcgagacc ggactccgtg tgaaggccat ggacgtgaac cccctctcgt
     3721 tctactacgc cgcgcaccgt ctcgggcgcg atcagaccgg gcccggattc gtcgcgttcg
     3781 tcgacatcgg cgcgtcgcgc ctggacatca acatcgccaa acagggcacg ctccgtttct
     3841 ctcggagcgt tgcgttgggc ggcgatacgt tgacggagaa cctcgcccgc gctctcggcg
     3901 ttgatcaggc gcaagccgag gggattaaac gggaggaagg cctgtccggg caggccaaag
     3961 tactcgatgt gctcgggccg gacgtggatc gattggtggt ggagattcag cggtccgtag
     4021 attactaccg cgcgcaagcg cacgacgggg ctcttgaagc gctctggatc ggcggaggca
     4081 cggggttgat ccccggattt gtcgcgtatg tcgggaagtt tttcgacgcg aatgtccgtc
     4141 tgttcaaccc attcgaagg
//
LOCUS       DBNGTT010000103         8216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2193221, whole genome shotgun sequence.
ACCESSION   DBNGTT010000103 DBNGTT010000000
VERSION     DBNGTT010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2193221"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="AC16BC_02375"
     CDS             <1..1091
                     /locus_tag="AC16BC_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3240490.1"
                     /translation="GTTSVARCGLATTAKTWHLFWRIARPAHRSPFQWVVQPVSVEER
                     EITVSGTTFRVREYRPRTHAATILLNGGFVVESIDDARLINFAMALAEIGFLVMTPDY
                     PAVRSLEFDPKTIDQIAGVIARLDQSATQGGRTPLAVLGLSYMGTLSLKAALRPELPY
                     SPELVAVLGGYADFGDLMHDVFRDVYRTDGVKVHVDPYGRFLVLRSAIGYLDAPPAER
                     ERIREILLAMGRQLDSTGVERAVQALSPEGAACVEALRRFHPDRQTQQWRRIMEGSRE
                     LIEALSVTESPERLRSRLMILHSVYDHILPCSGSIALYRRFPTADLVLTTLFTHVNVR
                     FSPRALWTQIRELRALFRMFGRIIALQG"
     gene            1221..1604
                     /locus_tag="AC16BC_02380"
     CDS             1221..1604
                     /locus_tag="AC16BC_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008556570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3240491.1"
                     /translation="MSQIAAIGLVATLVMMSGCRGGGNPASEAVAAAEVPTEHADGKR
                     LFDTYCARCHGVHAAGTGRGPSLLSKIYEPSHHGDEAFVLAAKRGVSAHHWGFGNMPP
                     IPGISDDMAKIIGYVRWAQRVAGIS"
     gene            1668..2696
                     /locus_tag="AC16BC_02385"
     CDS             1668..2696
                     /locus_tag="AC16BC_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3240492.1"
                     /translation="MIPPPTRSLTRRHLLAATAALVGAGLTGPAEAGVFFWGRLFSVP
                     PRDTPFLTPNDQFYRVNYSDHSLELAATVRSADWSLRLHGAVEHPRTFQYVDLLEQRT
                     MEQMVTLQCIDNEPGGSLMSNALWTGFPLARVLEAAKPLDTAMDVVFHSLDGYHDSIT
                     LDRALRGDALLAHSMNGVPLPKDHGYPLRAIVPGVFGIKNVKWLTEIEVVERDHKGYW
                     QERGWTDEGLIPVTSRIDSPGHYQLLRGGRQEIRGIAFAGLHGIKRVEVSVDGGRTWR
                     DAILAPVPRYAWVPWSYEWRVPAAGAHTIAVRAEGLDGVRQVEVASRAYPSGTSGLHT
                     IVALVESV"
     gene            2737..5037
                     /locus_tag="AC16BC_02390"
     CDS             2737..5037
                     /locus_tag="AC16BC_02390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3240493.1"
                     /translation="MDDLGFVTIEGLVAFGEALARREPRVAGVSRPALVPRSDRVSRA
                     VEAMPAVRAFVERVSTGAPDAEGYRAACRSLLESGCGGDLLVLFAAWNRALADGALAP
                     LLRASIGHTAKPNRRRPVAIVPRAQLTPQLIGERIVLDLGDDRFWLLPRDLSGRAVLF
                     ALRHGASMVESGTYRVGRRLANTLDPARGVVGADSVGRALAQMVGIVGRQLDFLRVPN
                     YLDPGTFTHYVSSSPNTTQLYERVAVALALQPAPPPRADAALESQDFGWATGLEKVIE
                     IEEAARAFGVDPTIGKRLIKHPLYGYPGGHSFFDLYVDVVDGIHRLGVTHQGGVACLY
                     THSSTLRALIVYLDARPFREAFADFGEYKEGQDNVVLLTSERGELSGYSTAVGLSAHE
                     RTAREAWATVERERRDRVILAPRQIKRVVALVSGGDFAGAGAALKELRVSGEQHGVAV
                     HFVRHGFLGLVNNWIEPVSEADTRGMANHSSSPIGSSRFEEFKDERVQRIAMTHLEPY
                     ATDGALVVIGGDGSLRGARALFEGFGMQVVGLPGSIDNNLDRTTSLGFHSAVALANQS
                     LESLKATSAAMGSIFLVEVMGAGSGHLALACAYQARAEGVLVNEHPEPDAYIDEVMLG
                     TLRRSLGVPNKSHLFVVAERTPHRHHPDGGVHGLVNYVAGTIADWPQLRMTPNGYPLS
                     VATKATILGHTLRGAPPVPEDKALAQFLAYETVRLLVEAPDTIVGCLASSRGEGDFAA
                     VPLHAVSPKPFDWAVFARMHGVVHRT"
     gene            5184..5390
                     /locus_tag="AC16BC_02395"
     CDS             5184..5390
                     /locus_tag="AC16BC_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3240494.1"
                     /translation="MPPTRKRSYKAQEICDTFDISKATLFRWERQGLIGGVGRDWRNW
                     RIYSEKNCREIERLIKRRVVQRER"
     gene            5404..6468
                     /gene="pilM"
                     /locus_tag="AC16BC_02400"
     CDS             5404..6468
                     /gene="pilM"
                     /locus_tag="AC16BC_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIR3240495.1"
                     /translation="MLFHRAQPVIGLDIGSNAIKLLQLKSTKKGYVVEKFGIKTIDPE
                     LIVDGAVMDAGRVVDILKELLREQTVKAKDVVISVSGHSVIVKKISVPPMTEEELDES
                     IKWEAEQYIPFDVNDVNLDFHILPSSDVPEGKEAMTVVLAAVKKDRLSEYTALVSEAG
                     LNPVVVDVDAFTLENVYDLAYGSTGSEVVALVNIGASVMNIHIVKGGNFSFTRDISTG
                     GNRYTETIQRDLNVSYEAAERAKRGENVEGVNPNALAEVINTMNGELAAEIGRSFDYF
                     RSTSTQETIDRVLLSGGTAKLAGLVPFLSERLGVPVELLDPFRNIRVNPKTVSPDLIA
                     EVGPQATVVVGLATRRPDDR"
     gene            6465..7013
                     /locus_tag="AC16BC_02405"
     CDS             6465..7013
                     /locus_tag="AC16BC_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR3240496.1"
                     /translation="MIKVNLLGVERPKRARRSIKGPSRLVGVGVIAVGLLGGMGYVWW
                     SMSARISSLEQQKVQRNQELATLKAKVKEVENFEANKKSYEEKIAIIEQLRKNQSGPV
                     RLLDEVSKSLPSRVWLTSLTEQAGKVEVEGRAVTNAEIVDFINNLKASRFIREILLIE
                     SRQVSEGGIPVYNFKLKCDLVV"
     gene            7018..7701
                     /locus_tag="AC16BC_02410"
     CDS             7018..7701
                     /locus_tag="AC16BC_02410"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR3240497.1"
                     /translation="MAFNVDLSSFKALPNYQKAVLVAVPVIMLLAAFVWFVYMPKNAQ
                     IVAIEQQIAQINNEINVNEAKARRLDELKRENAALDKHLTLLKEQLPPESEVATLLKQ
                     VSDLGIKTGLDFKLWKPADRRPGSSGLYTEIPVEVEVAGGYHALGAFFDRIGKLPRIV
                     NVSGLKMSNVKSDKDRLMIQTTFRATAFAAIDEAAAPTGGKLPAGAKPPEGQKAPVPA
                     PPKPGGKGA"
     gene            7592..>8216
                     /locus_tag="AC16BC_02415"
     CDS             7592..>8216
                     /locus_tag="AC16BC_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HIR3240498.1"
                     /translation="MKPRRRPAASFRPGPSRRKDKRRPCPPPPNREERVRNRAVLSPL
                     AWFLGLALLASCSESAPPQPTAKTTPAKPPGPVAVSPVTTPSAVPGGETVVALEAAVP
                     PPAQEAYSYNPSGRRDPFKSLIVASAQKKNIDLLPPLQRREVADLKYVAVVWGELGTY
                     AMLEMPDGKGYAVRVGTRVGPNRGVVKRITARDLTVVERYVDFFGETR"
BASE COUNT         1512 a         2356 c         2804 g         1544 t
ORIGIN      
        1 ctggcacaac ctcggtcgca cgatgtggct tggcgacgac ggccaagacg tggcatctgt
       61 tctggcggat cgcgaggccg gcgcatcgct cgccatttca gtgggtggtt cagcccgtgt
      121 cggtggagga gcgcgagatc acggtctcgg gcacgacctt tcgggttcgg gagtaccggc
      181 cgcggaccca cgccgccacg atcctgctga acggcggttt cgtcgtggag tccatcgatg
      241 atgcgcgcct gatcaacttc gcaatggcat tggcggagat cgggtttctg gtgatgaccc
      301 ccgactatcc cgcggtccgc tcgttggaat tcgatccgaa gacgatcgat cagatcgccg
      361 gggtgatcgc tcggctcgat cagtccgcga cgcagggtgg gcggaccccg ttggcggtac
      421 tcggcctgag ttacatgggg acgctcagtc tcaaggcagc gctgcgaccg gagttgccgt
      481 attctccgga gctggtcgcg gtgctgggcg ggtatgccga cttcggcgat cttatgcacg
      541 atgtctttcg cgacgtctat cgcaccgacg gcgtcaaagt gcacgtcgat ccgtacgggc
      601 gattcctggt gttgcggtcg gcgatcgggt acctggatgc gccgccggcg gaacgggaac
      661 ggatccgcga gattctcttg gcgatggggc gccaactcga ttccaccggg gtcgagcggg
      721 cggtccaggc gttgtcgccc gagggcgccg cgtgcgtgga ggcgctgcgg cgtttccacc
      781 cggatcggca gacgcagcaa tggcgccgta tcatggaggg gtcgcgtgag ctgatcgagg
      841 cgctgtctgt gaccgagtct ccagaacgcc tccgcagtcg cctcatgatt ctccacagcg
      901 tctatgacca cattcttccg tgctccggca gcattgccct ctatcggcgc ttcccaacgg
      961 ctgacttggt gctcacgacg ctgtttaccc acgtgaacgt gcggttttcg ccccgggcgc
     1021 tctggacgca aatacgcgag ttgcgcgcgc tgtttcggat gttcggccgg atcatcgcgc
     1081 tgcagggatg acccggcgtt gcctttcgtc gggttcgtcg actatcatgg cgcgcgatga
     1141 tcaggggggt gccaatggat gtcagccgca agggggccat tgtgcggggc cgtggggacc
     1201 gtcgctgttt cggaacgcga gtgagccaga tcgccgcgat cggtctcgta gcaacactcg
     1261 tgatgatgtc gggttgtcgc ggcgggggca atccggcctc ggaggcggtc gcggccgccg
     1321 aggtgccgac ggagcacgcg gacggcaaac ggttgttcga cacgtattgc gcgcgttgcc
     1381 acggcgttca cgcggcgggg accggccgag gcccctcatt gctgtccaag atttatgagc
     1441 cgtcccacca cggggacgaa gccttcgtgc tcgccgcgaa gcgcggcgta agcgcccacc
     1501 actggggctt cggcaatatg ccgccgatcc cgggaatctc cgacgacatg gcgaagatca
     1561 ttggctacgt gcggtgggct cagcgcgtcg cggggatatc gtaacccccc gcggcaccgg
     1621 cagggcctga accgccggcc cacttcgcag gacgcaagga gccgcggatg atccctccgc
     1681 cgacgcgttc actcacccgt cgccatctac tcgcggcgac cgccgcactg gtcggcgccg
     1741 gactcactgg gccggccgaa gcgggggtgt ttttctgggg gcggttgttt tcggtcccgc
     1801 cccgcgacac gccgtttctg accccgaacg atcagttcta ccgcgtcaat tacagcgatc
     1861 actccctgga gttggccgcc accgtgcggt ctgccgactg gtcgctcagg ttacacgggg
     1921 cggtggagca tccacgcacg ttccagtacg tggacctttt ggagcaacga accatggagc
     1981 agatggtgac gctccagtgt atcgacaacg aacctggcgg aagcctgatg agcaacgccc
     2041 tctggacggg atttccgttg gcgcgtgtgc ttgaagccgc caagccgctc gatacggcga
     2101 tggacgtggt gtttcacagc ctggacggct accacgacag catcacgttg gatcgcgcgc
     2161 tgcgaggcga cgcgctcctg gcgcactcga tgaatggcgt tcctctcccg aaggaccacg
     2221 ggtacccact gcgcgcgatc gtgccgggag tattcggaat caagaacgtc aagtggttga
     2281 ccgaaatcga ggtcgttgag cgggatcaca aggggtattg gcaagaacga gggtggaccg
     2341 acgaaggcct catcccggtg acctcgcgga tcgatagccc cggacactac cagctcttgc
     2401 gaggcggccg gcaggaaatc cgtggcatcg cctttgcggg actgcacggc atcaaacggg
     2461 tggaagtgag cgtggacggc ggccggacat ggcgcgatgc gatactggcg cccgttccgc
     2521 gatacgcatg ggtgccctgg tcgtacgagt ggcgggtgcc ggccgcgggg gcgcacacga
     2581 tcgcggttcg cgccgaagga ctggacgggg tgcgacaggt ggaggtggcg tcccgcgcgt
     2641 atccgtcagg gacatcgggc ctgcatacga tcgtggccct ggtggagtcg gtatgagatg
     2701 ccgcgtgctg gtggcggcgg ggcggggaga ttcgtgatgg acgatttggg attcgtcacc
     2761 atcgaggggt tggtggcgtt cggcgaagcg ttggcgcgtc gtgagccgcg cgtggcgggg
     2821 gtcagtcgtc ccgcgctggt gccccgatcg gatcgggtga gccgggccgt cgaggcgatg
     2881 ccggcggtgc gggcgttcgt ggagcgggtg agcaccggtg caccggatgc ggaggggtat
     2941 cgcgcggcct gccgatcgct gttggagtcc gggtgtggcg gggacctgct ggtcttattc
     3001 gccgcgtgga accgcgcgct cgccgacggg gcgctggcgc cgctgctgcg agcgtcgatc
     3061 gggcacacgg cgaagccgaa ccgccggcgt ccggtggcga tcgtcccgcg cgcgcagctg
     3121 accccgcagt tgatcgggga gcgaatcgtg ctcgacttgg gggacgaccg gttttggttg
     3181 ttgcctcgtg atctgtcggg ccgggccgtg ttgtttgcgc tgcgacacgg ggcgtccatg
     3241 gtggagagcg ggacctaccg agtagggcga cggttggcca acaccctgga tccggcgcgg
     3301 ggagtagtcg gggcggattc cgtgggccga gccctcgcgc aaatggtggg aatcgtcgga
     3361 cggcaactgg atttcctccg cgtccccaat tatctcgacc cggggacgtt cacgcactat
     3421 gtgagttcga gccccaacac cacgcagctg tacgaacgcg tggccgtggc gctcgccctc
     3481 caaccagcac caccgcctcg cgcggacgcg gcgttggagt ctcaggattt cggatgggcc
     3541 acgggtcttg agaaggtcat cgaaatcgaa gaggccgcgc gtgcgttcgg cgtcgatccg
     3601 acaatcggta aacgcttgat caagcaccct ctctacgggt acccgggcgg gcactcgttc
     3661 ttcgatctgt acgtggacgt ggtcgacggc attcaccggc ttggagtgac gcaccaaggc
     3721 ggcgttgcct gcctgtacac gcacagctcc accctgaggg cattgatcgt gtatctcgat
     3781 gcccgcccgt ttcgcgaggc gttcgcggac ttcggcgaat acaaggaagg acaagacaac
     3841 gtcgtgctct tgacgagcga acggggcgaa ctctccggct actccacggc ggtggggctg
     3901 tccgcgcacg aacgaaccgc gcgtgaggcg tgggccacgg ttgaacgtga acgacgggac
     3961 cgggtcatcc tcgcaccccg ccaaatcaaa cgcgtggtgg cgttggtatc gggaggagat
     4021 ttcgcggggg ccggcgcggc gctcaaggaa ctgcgggtaa gcggcgaaca acatggcgtg
     4081 gccgtccatt tcgtgcgcca cgggttcctg gggctggtca acaactggat tgagccggtg
     4141 agtgaagcgg acacgcgcgg catggcgaat cattccagca gtccgatcgg cagtagccgc
     4201 tttgaggaat tcaaggacga gcgcgtccag cggatcgcca tgacgcacct ggaaccctac
     4261 gcgacggatg gcgcgctggt ggtgatcggc ggtgacggca gtctgcgtgg cgcgcgggcg
     4321 ctcttcgagg ggttcggcat gcaagtggtg ggtctcccgg gcagtatcga caacaacctc
     4381 gatcgcacca cgtcgctggg attccactca gccgtggcgt tggccaatca gtcgctggaa
     4441 tcgctcaagg cgaccagcgc ggccatgggc agcattttcc tggtcgaagt gatgggcgcg
     4501 ggctctggcc acttggcact ggcctgcgcc tatcaggcgc gagccgaagg ggtgctggtc
     4561 aacgaacatc cggagcccga tgcctatatc gacgaggtga tgctcgggac gctgcgtcgg
     4621 agcttgggag tgcccaataa gagtcatttg ttcgtggtgg ctgagcgcac cccgcatcgg
     4681 caccatccgg acggcggcgt acacggactg gtgaactacg tcgcggggac catcgcggac
     4741 tggccccaac ttcgcatgac gccgaacggg tatccgttga gcgtggcgac caaagccacg
     4801 atcttggggc acacgttgcg cggcgcgcct cccgttcccg aagacaaggc cctcgcgcag
     4861 ttcttggcct atgaaaccgt tcgtctgctg gtggaagctc ccgacacgat cgtcgggtgt
     4921 ctcgcgagtt cccgtggaga aggagacttc gcggcggtcc ccctgcacgc ggtgtctccc
     4981 aagccgtttg actgggcggt gtttgctcgg atgcacgggg ttgtgcatcg gacataacgg
     5041 cccgtgtgcg gacccgggag tcgggctcag gcgaatcttc tttgcggtgc cggaattccc
     5101 cttgacagga tgagcatact aagtatactt acagccgttt cgtggaagat cccaaaacgg
     5161 attgccgacg agaatcgaca acgatgccgc caacccgtaa acgatcgtat aaggcacaag
     5221 aaatttgcga cacgttcgac atctccaagg cgacgctgtt tcggtgggag cgccagggtc
     5281 tgatcggcgg ggtcggccgc gattggcgca attggcggat ctatagcgag aagaattgtc
     5341 gggagatcga acgcttgatc aaacgacggg ttgtccaacg agaacgatag gaacgcgacg
     5401 tctatgctct ttcaccgcgc gcaaccggtc atcggactcg acatcggatc caatgcgatc
     5461 aagctgctcc agttgaagtc cacgaagaag gggtacgtgg tggaaaagtt cgggatcaaa
     5521 acgatcgatc ccgaactcat cgtggacggg gccgttatgg acgcgggccg cgtcgtggac
     5581 atcctcaagg aactgctccg cgaacagacg gtcaaggcca aggacgtggt gatttcggtg
     5641 tccgggcact ctgtgatcgt gaaaaagatc agtgtgccgc cgatgacgga ggaagaactc
     5701 gacgagtcca ttaaatggga ggcggaacaa tatatcccct ttgatgtgaa cgacgtcaat
     5761 ttggacttcc atattctgcc gtcgtcggat gtaccggaag gcaaggaggc gatgacggtg
     5821 gtgctcgccg cggtcaaaaa ggatcgactg tcggagtaca ccgcgttggt gtcggaggcc
     5881 ggactgaacc cggtcgtggt ggatgtcgat gcctttacct tggagaatgt ctatgacttg
     5941 gcctacggat cgacggggtc cgaggtcgtc gccctcgtca acatcggcgc aagcgtcatg
     6001 aacattcaca ttgtcaaggg cgggaacttt tccttcaccc gcgacatctc gacgggcggc
     6061 aatcgatata cggaaacgat ccagcgggat ttgaacgtca gttacgaggc cgccgagcgc
     6121 gccaagcgcg gtgaaaacgt ggagggcgtc aatcccaatg cgctggccga agtgatcaac
     6181 acgatgaatg gagaactcgc ggcggaaatc ggccgctcgt tcgattactt ccgctcgacg
     6241 tcgactcagg agacgatcga ccgggtgttg ctcagcggcg gtaccgcgaa gcttgccggg
     6301 ttggtcccgt tcttgtccga acgactcgga gtcccggtcg agctgttgga cccctttcgt
     6361 aatattcgag tcaatccgaa aacggtgagt cctgacctga ttgcggaggt tggcccgcag
     6421 gccacagtgg tggttggcct cgcgactcgc cggcccgacg accgatgatc aaagtcaatc
     6481 tcctgggcgt tgagcgcccg aaacgagcgc ggcggtcgat caaggggccg agccgccttg
     6541 tcggcgttgg agtcatcgcg gtcggtctgt tgggcggcat gggatacgtg tggtggtcga
     6601 tgtcggcccg gatctcttct cttgagcaac agaaggttca gcggaaccaa gagcttgcga
     6661 cgttgaaggc gaaagtcaaa gaggtcgaga atttcgaggc gaacaagaag agttatgaag
     6721 aaaagatcgc gatcatcgaa caactccgga agaaccagag cgggccggtc cgactcctgg
     6781 atgaagtgag taagagcctg ccgagccggg tgtggttgac gtcgttgacg gagcaggctg
     6841 ggaaggttga ggtggagggg agggcggtta ccaacgcgga gatcgtggac ttcatcaata
     6901 atctcaaagc gtcccgtttt atccgcgaga ttctgctgat cgaatcccgg caggtcagtg
     6961 agggaggtat ccccgtgtac aacttcaagc ttaagtgcga cttggtggta tagcctgatg
     7021 gctttcaatg tagacctcag ctctttcaag gccctgccca actatcagaa ggccgtgctg
     7081 gtggcagttc ccgtcatcat gttgttggcg gcgttcgtct ggttcgtgta catgccgaag
     7141 aatgcccaga tcgtcgccat cgaacaacag attgcccaga ttaataatga aatcaacgtg
     7201 aacgaggcga aggcccgacg actggacgag ctgaaacggg agaacgccgc gttggataag
     7261 catctgacat tgttgaagga gcagctccct ccggaatccg aagttgccac tctcttaaaa
     7321 caggtttccg atctcggcat caaaaccggt ctggacttca aactctggaa gccggcggat
     7381 cgacgaccgg gctcttcggg cctctatacg gagatcccgg tcgaagtgga agtcgccggc
     7441 ggctaccatg ccttgggggc attcttcgac cgcatcggga aattgccgcg gattgtcaat
     7501 gtgtcgggtc tcaaaatgtc caacgtgaaa tctgacaagg accgtctgat gattcagaca
     7561 acctttcgcg caaccgcgtt tgccgcgatt gatgaagccg cggcgccgac cggcggcaag
     7621 cttccggccg gggccaagcc gccggaagga caaaaggcgc ccgtgcccgc cccccccaaa
     7681 ccgggaggaa agggtgcgta accgcgcggt cctcagcccg ctggcgtggt ttcttggcct
     7741 cgcgctgctt gcgagttgct ccgagagcgc gccgccgcag ccgacggcca agacgactcc
     7801 cgcaaagccg ccgggcccag tggcggtgag tccggtaacg acgccatccg cggttccagg
     7861 cggggagacg gtggttgcgc ttgaagcagc agtgccgccc ccggcccagg aggcgtactc
     7921 gtataaccct tccggtcgcc gtgatccctt caagtcgctg atcgtggcta gcgcacagaa
     7981 gaagaacatc gacctgctgc cgccgcttca gcggcgtgaa gtcgctgatc tgaaatacgt
     8041 cgcggtggta tggggagaac ttgggaccta cgccatgctg gaaatgccgg atggaaaggg
     8101 atacgcggtg agagtgggga cgcgggttgg ccccaaccgt ggggtggtca agcggatcac
     8161 ggcgagagat ctgaccgtgg tggagcggta cgtggatttc tttggcgaga cacgat
//
LOCUS       DBNGTT010000104         3926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2448544, whole genome shotgun sequence.
ACCESSION   DBNGTT010000104 DBNGTT010000000
VERSION     DBNGTT010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2448544"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16BC_02420"
     CDS             <1..237
                     /locus_tag="AC16BC_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240499.1"
                     /translation="GNANESLIRNMNTNGTASSDTWCAGGRAQIAPDTLGAAPPEIKK
                     LLAEFGRDLASGRLATGAARGAQLAADAHRHPDP"
     gene            249..1046
                     /locus_tag="AC16BC_02425"
     CDS             249..1046
                     /locus_tag="AC16BC_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3240500.1"
                     /translation="MDKGWLVVAAVVIAVAAAVLFTTSSPGEPPEGMVLIPGGTFLMG
                     SRGDEGKLGFEVGVDEMPQHSVRVRSFFMDRTEVTNAQYQKFIEVSGRPAPADPKDVE
                     YYAWVDGKPPAGQEEHPVCYVAWADADAYCRWVGKRLPTEAEWERAARGDDNRIWPWG
                     NTFDPTRCNTTEAGFRWSTPVGSFPEGASPYGVLDLCGNVAEWTSSWYEPYPGSTLQR
                     AAFGRKMRVARGGAWVLTAQPWSRTTNRNLAQPPDLRHRTLGFRCVK"
     gene            1268..1906
                     /locus_tag="AC16BC_02430"
     CDS             1268..1906
                     /locus_tag="AC16BC_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3240501.1"
                     /translation="MGSSPNPKLAWFEQGVDEAPQHRVKVPAFWIDRYEVTEQTYAAF
                     VSATGHRAPRVWADGRQPRRDHPVVDVSWDDADAYCRWAGKRLPTEAEWEKAARGDDN
                     RIWPWGNEYKRGAALLQDTASGDTAAVGSHPEDKSPYGVYDMAGNAMEWTASWYEKYP
                     ASTLDRPDFGEKYRVLKGGAWTTPPLPFSRAANRHAISPFWDHPHFGFRCAK"
     gene            1894..2340
                     /locus_tag="AC16BC_02435"
     CDS             1894..2340
                     /locus_tag="AC16BC_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3240502.1"
                     /translation="MREMMEPGKGGTGEPGKVRKGEPGKLIESFKELRVYQGAMDAAM
                     KVFEATKTFPPEEKYAMVDQIRRSSRSVCANLAEAWRKRRCPAAFVAKLNDAESEACE
                     TQVWIEFARKCRYLESSTSNELDQVYDRLIAQMVRMIDESPKWLIK"
     gene            2466..3179
                     /locus_tag="AC16BC_02440"
     CDS             2466..3179
                     /locus_tag="AC16BC_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3240503.1"
                     /translation="MILIPAGPFWMGSDAGEGAVGIDVGVDESPRHQVNLPAFYIDRY
                     EVTNAQYADYVNAVGSAYRPAPWRERDALEKEPNHPVADTDYYDAEAYCRWAGKRLPT
                     EAEWEKAARGPDGATYPWGSTFSRDKANTQESGLAWSRPVGSYPQGASVYGVEDLIGN
                     VWEWTSSWYERYPGSTVQRAAFGKKYHVLRGGSWGAPAVPFARAANRHAPELFAVNDR
                     DEDWHTGYDVGFRCVKDAR"
     gene            complement(3211..>3926)
                     /locus_tag="AC16BC_02445"
     CDS             complement(3211..>3926)
                     /locus_tag="AC16BC_02445"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF018533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR3240504.1"
                     /translation="ALRGETIDAVDRRGKHVLFSLRRTSLVINPMLVGRFYYQPPEAK
                     PPRQTVMDLRLSTGKALRYADERQMGRLYLVPDRDYAKIPGFLEQGPEPLADDFTFER
                     FLDRLKGRYGEIKGLLTNAKVIAGIGNAYADEILFEAGIYPFRRKKDLSVEELRRVYD
                     AIRSVLPRASRIVAERMGAQIHLKIRDFLAVHGKGEQPCPRCGQRIATIGRERATNFC
                     RACQPGLMVAPQRSLPLGE"
BASE COUNT          709 a         1264 c         1329 g          624 t
ORIGIN      
        1 ggcaatgcga atgagagtct gatccggaac atgaacacca acggcacggc ctcgagcgac
       61 acgtggtgcg cgggcggacg agcgcagatc gcgccggata cattgggcgc tgcgccgccg
      121 gagatcaaga aactgctggc cgaattcggc cgcgaccttg cgtcgggacg actggccacg
      181 ggcgccgcgc gcggagccca gctcgcggcg gatgcgcacc ggcacccgga tccgtagcgc
      241 gatgatccgt ggataagggg tggctggtcg tggccgcggt cgtcatagcc gtggccgcgg
      301 cggtcctgtt caccacgtcg agccccggcg aaccgcccga gggtatggtg ctcattcccg
      361 gcggaacgtt tctgatgggc agccgcggcg acgaaggcaa gctgggattc gaggtgggcg
      421 tggacgagat gccccagcac tcggttcgcg tgcggtcgtt cttcatggat cggaccgaag
      481 tcaccaacgc gcagtatcag aaattcatcg aggtcagcgg ccgtcccgcg cccgccgacc
      541 ccaaggacgt ggaatactac gcatgggtgg acggcaagcc gccggccgga caagaagagc
      601 atcccgtgtg ctacgtggcc tgggcggacg cggacgcgta ctgccggtgg gtgggcaagc
      661 ggttgccgac cgaggccgag tgggagcgcg cggcgcgggg cgacgacaac cggatctggc
      721 cctggggcaa cacgttcgat cccacgcgat gcaatacgac cgaagcggga tttcggtggt
      781 ccacgcccgt gggcagcttt cccgaaggcg ccagtccgta cggcgtgctg gatctgtgcg
      841 gcaacgtagc ggagtggacc tcgtcctggt acgagccgta tcccggcagc accctgcaac
      901 gcgcggcgtt cggccggaag atgcgggtcg cgcgcggcgg cgcctgggtg ctcaccgccc
      961 agccctggtc gcgcaccacg aaccgcaacc tggcgcagcc gccggatctg agacatcgca
     1021 ccttgggatt caggtgcgtg aagtgatcga accggggaat cggggaaccg gggaaccgga
     1081 gagggactac aggagcaatg acgaaccggt cctcgccttt acgccgattc cccgattcgc
     1141 cggttccccg gttcgtctgt ccgtccttac attaatgatt gcctgctctc atctcccctt
     1201 cgccggttcc cccgctcccc ccaccgctcc ggagggaatg gtgttcattc cctccggagc
     1261 ctttcagatg ggtagctccc ccaaccccaa gctcgcctgg ttcgagcagg gcgtggacga
     1321 ggcgccgcag catcgcgtga aggtgcccgc gttctggatc gaccgctacg aagtgaccga
     1381 acaaacgtac gccgcgttcg tgtccgcgac cggacaccgc gcgccgcgcg tgtgggccga
     1441 tggccggcag ccgcgccggg atcatcccgt ggtcgatgtc tcgtgggacg acgcggacgc
     1501 gtactgccgg tgggcgggca agcggttgcc caccgaggcc gagtgggaaa aagccgcgcg
     1561 gggcgacgac aaccggatct ggccgtgggg caacgagtac aaacgcggcg ccgcgttgct
     1621 gcaggacacg gccagcggcg acaccgccgc ggtgggcagc caccccgagg acaagagccc
     1681 ctacggcgtg tacgacatgg ccggcaacgc catggagtgg accgcgtcgt ggtacgagaa
     1741 atatccggcc agcacattgg accggccgga tttcggcgaa aaataccgcg tgctcaaagg
     1801 cggcgcctgg accacgcccc ccctgccctt cagccgcgcg gccaaccgcc acgccatctc
     1861 cccgttctgg gaccacccgc attttggatt cagatgcgcg aaatgatgga accggggaag
     1921 gggggaaccg gagaaccggg gaaagtgcgt aaaggcgaac cggggaaact gattgagagt
     1981 ttcaaagaac tacgagtcta ccaaggcgcg atggatgcgg ccatgaaggt attcgaggcg
     2041 accaagacct ttcctccaga agagaagtac gccatggttg atcagatccg gcgctcatca
     2101 cgttccgtgt gcgccaatct cgctgaggcg tggcgcaaac ggaggtgccc ggcagccttc
     2161 gttgccaagc tcaacgacgc ggagagcgaa gcttgcgaga cccaggtgtg gatcgagttt
     2221 gcgcgaaagt gtcggtatct ggaatcctcc accagcaatg aacttgacca agtctacgat
     2281 cgcttgatcg cgcaaatggt tcgcatgatc gacgagtcgc cgaaatggct aatcaaatga
     2341 agggacccct catgcctaac cgacttattg tcctcgtcct gagcgcaagc ctgatgttca
     2401 tttcctgcac ttctctcccc atcccccctt ccccccaccc cccgctcgcc tctggcccgg
     2461 cgggaatgat cctcattcca gccgggccct tctggatggg cagcgacgcg ggagaaggcg
     2521 cggtggggat cgacgtcggc gtggacgaga gcccgcggca tcaggtcaat ctcccggcgt
     2581 tctacatcga ccgctacgaa gtgaccaacg cgcagtacgc ggactacgtg aacgccgtgg
     2641 ggtcggccta tcgccccgcg ccgtggcgcg aacgcgacgc gttggaaaag gagccgaatc
     2701 acccggtcgc ggacaccgac tactacgacg cggaagccta ctgccgttgg gcgggtaagc
     2761 gtctgccgac agaggccgag tgggagaagg ccgcgcgcgg tccggacggc gcgacgtatc
     2821 cgtggggatc gaccttttcg cgcgacaagg ccaacaccca ggagagcggc ctggcctgga
     2881 gccgacccgt gggcagctac ccgcagggcg cgagcgtgta cggggtggaa gatctgatcg
     2941 gcaacgtgtg ggagtggacc agttcgtggt acgagcggta ccccggcagc acggtgcagc
     3001 gcgccgcgtt cgggaagaag taccacgtgc tgcgcggcgg ctcgtggggc gcgcccgccg
     3061 tgccgttcgc gcgcgcggcc aatcgccacg cgccggagtt gttcgcggtc aacgaccgcg
     3121 atgaggactg gcacacgggg tatgacgtgg gctttcgctg cgtgaaggac gcgcgctgag
     3181 gaactcgcgg aggccgttgc tcgaccgtct ctactcgccc aaaggcaatg accgctgcgg
     3241 cgccaccatg agccccggtt ggcacgcgcg gcaaaagttc gtggcgcgtt cgcgtccgat
     3301 ggtcgcgatc cgctgcccgc agcgcgggca gggctgctcg cccttgccgt gcaccgcgag
     3361 gaagtcgcgg atcttgaggt ggatctgcgc gcccatgcgc tcggccacga tccggcttgc
     3421 gcggggcaac acgctgcgga tcgcgtcgta cacgcgccgc aattcctcga ccgacaaatc
     3481 ctttttcctg cggaacggat agatccccgc ctcgaacagg atttcgtccg cgtaggcgtt
     3541 gccgatcccg gcgatcactt tcgcgttggt cagcaggcct ttgatttcgc cgtaccggcc
     3601 cttgaggcga tccagaaacc gctcgaacgt gaagtcatcc gccagcggct ccgggccttg
     3661 ttcgagaaac cccgggatct tggcgtaatc tcgatccggc accaggtaga gccgtcccat
     3721 ctgacgctcg tccgcgtagc gcaaggcctt gccggtggac agccgcaggt ccatcacggt
     3781 ctgccgcggc ggcttcgcct ccgggggctg gtagtagaag cggcccacca gcatgggatt
     3841 gataaccaga ctggtccgcc gcagcgagaa caggacgtgt ttgccgcgcc ggtcaacggc
     3901 gtcgatggtc tcgccgcgca gcgcct
//
LOCUS       DBNGTT010000105         1876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1299292, whole genome shotgun sequence.
ACCESSION   DBNGTT010000105 DBNGTT010000000
VERSION     DBNGTT010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1299292"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="AC16BC_02450"
     CDS             complement(<1..212)
                     /locus_tag="AC16BC_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3240505.1"
                     /translation="MAKRLNIRISGLGGQGAVTSAHLLAMAANKDGKFAISNPFFGAE
                     KRMAPAESYARIGSERIYDRGEIVYP"
     gene            complement(227..1120)
                     /locus_tag="AC16BC_02455"
     CDS             complement(227..1120)
                     /locus_tag="AC16BC_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3240506.1"
                     /translation="MGKDKIRINEEFYDIMPVEYRDMVEDATYGKEDRGWKDIGVHKE
                     LIEEHSLCAGCPESMAFRYILASLPAPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIATGLKRALTVRFPNKVKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKKFDKVRLPEIARESGCHYVVNMTVSQPGRVEKV
                     IKNAVHIAREIGPTYVQLYTPCILEIGKQSMEGLQEMRDSEKPAQRFAYKEYISDEAK
                     AYLADLDAKRKAAAAQLAAKS"
     gene            complement(1113..>1876)
                     /locus_tag="AC16BC_02460"
     CDS             complement(1113..>1876)
                     /locus_tag="AC16BC_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3240507.1"
                     /translation="LEIGLIIDTGMMIWHAETAQDFFDWILMGYMVAEQPDVHIPLAL
                     CCDGFFVTHTKDTVNLTPADMTLPPYDPYRSPVPCMDMECPPVRMMRDPFIMKSNYIS
                     YATHASWQQEVRAACERSRKHANALLGGLIDTENVDAEILIVTSGTAVSQGREAIRLL
                     EAEGHKVGLVKVKALRPFPTEEIRQATKHAKWIIVPEFNVVGWMAREIAAVIERNDRV
                     VAGPHVAGGMTMPPEVIVEQIHKVIKLGKGALVHG"
BASE COUNT          322 a          620 c          539 g          395 t
ORIGIN      
        1 tcggggtaga cgatctctcc gcggtcgtag atgcgctccg acccgatgcg cgcgtaactt
       61 tcggccgggg ccatgcgttt ttccgcgccg aagaacgggt tggaaatcgc gaactttccg
      121 tccttattgg cggccatcgc caacaggtgc gccgaggtga ccgcgccctg cccaccgagg
      181 cccgagatcc ggatgttgag tcgttttgcc atcgccgtct cgctcctcag ctcttggccg
      241 ccaattgggc cgcggcggct ttccgcttgg cgtccagatc ggcaaggtag gccttcgctt
      301 cgtcgctgat gtattccttg taggcaaacc gctgggcggg cttctcggag tcacgcatct
      361 cctgcaaccc ctccatgctc tgcttgccga tctccaaaat gcagggcgtg taaagctgga
      421 cgtaggtggg accgatctcg cgcgcgatgt gcaccgcgtt cttgatcacc ttctccaccc
      481 ggcccggctg gctgacggtc atgttgacga cgtagtgaca gccggactcc cgcgcgatct
      541 ccgggagccg gaccttgtca aacttcttgc ccaccggcgc catcttggcc acgaagccct
      601 tctgcatcag gcccgactcc tgcccgccgg tgttggcgta cagctcgttg tcgaaacaga
      661 tggtcgtaaa cttctcctgc cggaaccacg cctgcagggt catgtccaaa ccgatatcca
      721 cggtcgcgcc gtcgccggcc agcaccacca cgtctttcac tttgttcggg aaacgcaccg
      781 tgagcgcgcg tttcagcccc gtggcgatcg cgttctggtt gccgaacagc gagtggatgt
      841 tgtggaccgc gaccatcggg aacaccaggc tggtgcagcc ggtcgagccg accatcaccg
      901 tgtcttccgg cgcgggcagc gacgcgagga tgtagcggaa cgccatggac tccggacacc
      961 ccgcgcagag cgagtgttct tcgatcaatt ccttgtggac accgatatcc ttccacccgc
     1021 ggtcttcctt accataggtg gcatcttcca ccatgtcccg atattcgacg ggcatgatgt
     1081 catagaactc ttcattgatc cggattttat ctttacccat ggacgagggc ccccttcccg
     1141 agtttgatga ctttgtggat ctgttcgaca atgacctcgg gcggcattgt catcccgccc
     1201 gccacgtgcg gtcccgccac aacgcggtcg ttgcgctcga tcaccgcggc aatttcgcgc
     1261 gccatccatc ccaccacatt gaactccggc acgatgatcc acttggcgtg tttggtggcc
     1321 tggcgaatct cctcggtcgg gaacggccgg agcgctttga ccttcaccag ccccaccttg
     1381 tggccctcgg cctccagcag acggatcgct tcccggccct gcgacaccgc ggtgccggac
     1441 gtcacgatca ggatctcggc gtcgacgttc tcggtatcga tcaagccgcc caagagcgcg
     1501 ttggcgtgtt tccgcgaccg ctcgcaggcg gcccggactt cctgctgcca cgacgcgtgc
     1561 gtggcgtagc tgatgtagtt ggacttcatg ataaacggat cacgcatcat gcgcaccggg
     1621 ggacattcca tgtccatgca cggaaccggc gaccggtacg gatcgtacgg cgggagcgtc
     1681 atgtcggccg gcgtcaaatt gaccgtatcc ttggtgtggg tcacgaaaaa cccgtcgcag
     1741 cacagggcca gggggatgtg gacatccggt tgctcggcca ccatataccc catcaaaatc
     1801 caatcgaaga aatcctgggc cgtctccgcg tgccagatca tcatcccggt atcgataatc
     1861 agcccgattt ccagcg
//
LOCUS       DBNGTT010000106         2246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2321638, whole genome shotgun sequence.
ACCESSION   DBNGTT010000106 DBNGTT010000000
VERSION     DBNGTT010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2321638"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..1029
                     /locus_tag="AC16BC_02465"
     CDS             481..1029
                     /locus_tag="AC16BC_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240508.1"
                     /translation="MNVLTPPTVYGRSIWSFLAPLTAMASLLALLTRRAASGWRRGIG
                     QGALLVILGVWSLGVALLAYHQGIALASDFGVFGRAARNEQYRFIDGAPLWEDMRDVH
                     ATLQTGDRVQFVVAGEAAAAFGGFWSLRAAYYLAPFPVGEINPHVRLVYFGTRHPSCE
                     EIGPGLAILQDGERFCLFRVQS"
     gene            1026..>2246
                     /locus_tag="AC16BC_02470"
     CDS             1026..>2246
                     /locus_tag="AC16BC_02470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3240509.1"
                     /translation="MTVLTAAGGIIVLVALGGVWRRGLAVFDRVSCIGLAFGLGTVYV
                     SAVLGAMALARIPLTPMAAIPATTIPAVVSWLLGRRTRDARTPYGTPYGVSVGHEWSR
                     RVDPWWWVGGVVLTLLGATAVVRALIKPIVGSDAWAEYARKAKWIYSESAIPRSMFES
                     VGYPNYPLGLPLQEVWMAWFAGSWDDVAIKLLFPAYLLALCLIVYGTIRDKAGPRLAM
                     MGTLFVAALPLMVQHAQDAYTDLPLAYFVLAGAVCLTRFVSSREPSALILASVFGMGA
                     IAMREDGVIPVAANALILAFVLIRSGHASTRSVLRDVVLYVGPPGAVWLTWSLIKLNL
                     GITSSVSLDLVEFPALLTRLLVLSKAVIFSFFVSGNWILLWVLFFIAVLAEIRSTLRK
                     EWVFMVWPVLLYLPA"
BASE COUNT          328 a          601 c          764 g          553 t
ORIGIN      
        1 actaaggatg cgtggacatt cgccgaatcc tcggagcccc tatggacctc tccgaatggg
       61 atggttacga ggatcgagcg ggggatgttg gtcactccga atcgtgagga agccgttgtg
      121 ttatttagcg gtccaaccac ccttaagatt gaccgataca tgtccgtgcg gattcgtctc
      181 gaaacggaac acccgtctga tgggcgcctt gcattgatga tccagacccc tgcggggact
      241 caacggaacg tcctgtcgtt tgccgtgcgg gggggggctg caggggacga gattcgtctc
      301 acgctgggtg acgagctcgg tcgaggtatg ctacgcgaaa tggcgcttct cccctcggca
      361 atcccgcagc gagtggtcgt ggcgtccatc gtattcgaac ctgctcggcc catcgaggcg
      421 atttggaggg aggtgacgtc gccggagcct ggatgggatg ccgcccgcgg gggacattcg
      481 atgaacgtgc tcaccccgcc gacagtgtat ggccggtcga tatggtcctt tcttgcacca
      541 ctcaccgcaa tggcatcctt gctggcgctg ctgacccgca gggccgcatc aggttggcga
      601 cgtggcattg gacagggagc ccttctggtc atcctcggcg tgtggagtct cggcgtcgcg
      661 ttgctggcgt atcatcaggg cattgcgctc gcgtccgatt ttggcgtctt cggtcgggct
      721 gcgcgaaatg aacaatatcg attcatcgat ggtgcgcctt tgtgggaaga catgcgagac
      781 gtccatgcca cgcttcagac gggtgaccgg gttcagtttg tcgtggcggg ggaggccgcg
      841 gcggcgttcg gcggtttttg gtcgttgcgg gcggcctatt acctggcgcc gtttcccgtc
      901 ggcgagatca atccgcatgt gcgactggtg tattttggaa cgcgtcatcc ctcttgcgaa
      961 gagatcggtc ccggcctggc catcctccaa gacggtgaga ggttttgttt gttccgggtg
     1021 caatcgtgac ggtgctgacc gcggccggcg gcatcattgt gctcgtggcg ttgggcggag
     1081 tgtggcgccg ggggctcgca gtgttcgacc gtgtgagttg catcgggttg gcgttcggcc
     1141 tcggaacggt gtacgtctcg gcggtgttgg gtgcgatggc gctggcacgg attcctctca
     1201 ccccgatggc cgcgatcccg gccacaacga ttccggcggt ggtgtcgtgg ttgttgggcc
     1261 ggcgaacgcg tgatgcgcgg acgccatacg gtacgccgta tggtgtgtcc gtgggccatg
     1321 agtggtcgag acgtgtggat ccgtggtggt gggtgggcgg cgtggtcctc acgctcttgg
     1381 gagcgacagc ggtcgttcgc gcgttgatca aacccattgt cggatcggat gcctgggccg
     1441 agtatgcgcg gaaagccaaa tggatttatt ccgagtcggc gattcctcgt tcgatgttcg
     1501 agtcggttgg atatccgaat tacccgcttg gtctccctct tcaagaagtg tggatggcct
     1561 ggtttgccgg atcatgggat gacgtggcga tcaaattgct ctttccggcc tatctgctgg
     1621 ccctgtgcct gatcgtctat ggaacgatac gcgacaaggc cggacctcgg cttgcgatga
     1681 tggggacgct cttcgtggcc gcgctgccgc tgatggtcca gcatgctcag gatgcctaca
     1741 cggacttgcc gctcgcctac ttcgtgttgg ccggcgccgt ctgtttaact cgttttgtgt
     1801 cttcccgcga accctccgcg ttgatcctcg catcggtctt cggaatgggg gcgattgcga
     1861 tgcgggagga cggcgtgatt ccggttgcgg ccaatgcgct gattttggcg ttcgtgttga
     1921 ttcgatcggg acacgcttcg acacgctccg tcctcaggga cgtggtcctg tatgtggggc
     1981 cacccggtgc ggtgtggctg acctggtctc tgatcaagct gaacctcggg atcacgtcga
     2041 gcgtgtcgct cgatctcgtg gaatttcccg cgcttctgac tcggttgctc gtgttgagta
     2101 aagcggtcat tttctcgttc tttgtgtcgg ggaattggat tcttctgtgg gtgttgttct
     2161 tcattgcggt tctggcggaa atccgatcca cgctgaggaa agaatgggtg ttcatggtct
     2221 ggccggtgct gctgtatctc ccggcg
//
LOCUS       DBNGTT010000107         4634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1043815, whole genome shotgun sequence.
ACCESSION   DBNGTT010000107 DBNGTT010000000
VERSION     DBNGTT010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1043815"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1020
                     /locus_tag="AC16BC_02475"
     CDS             136..1020
                     /locus_tag="AC16BC_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3240510.1"
                     /translation="MSLETLKISPGFESYMPKEYVDLVERGPYGREVKVSEMGTFKEV
                     LEEHPMCAGCAMTLFIRTALIGLPAPEHTVILGTAGCGRLALSQASIPFIYGNYGDTN
                     GVASGLKRGLEIRFPNQQKDVVVMAGDGGLSDIGFSCVMHSWFRKEKFTTVMLDNEVY
                     GNTGGQESGMTNKGVVLKMAPHGKKFEKMDMIGLAKTAGVAYIARLAPTNPTRIANVI
                     RKAVLIAREVGPTYVQAYTSCNIEYAIPTAKVMDDARDVEKDRYGFMEIISDDAKAYL
                     ERIEQEKKAKKAVSAPKA"
     gene            1061..1780
                     /locus_tag="AC16BC_02480"
     CDS             1061..1780
                     /locus_tag="AC16BC_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3240511.1"
                     /translation="MKRYNIRMAGIGGQGVVTASHVMSNGVIIAGGESTLVPFFGSEK
                     RMAPVESYVRVAEGRIYEIGEIIYPNVIMIFHTQVITHGKSYTMPFYSGLKDDGVVLI
                     NSEHPLTLEPDQERELREKRAKVLYIPASRLASEIGGTELATNMGMVGAVAGTIGLPD
                     LASLEQSVKERFLGKGFVVSGGTASLDNVIEKKFGKKVELIQKNMDVIKAAHKYALDH
                     KWNELTATQAAAANTLAAAAR"
     gene            1811..2074
                     /locus_tag="AC16BC_02485"
     CDS             1811..2074
                     /locus_tag="AC16BC_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3240512.1"
                     /translation="MYAVAQIDVDICSKTSCRLCTQFCPESNTINYDVKRNTAYVAVD
                     RCKGCEICVGICDNLAKHHAIKMVPITDVVGGSEISKLGFHKF"
     gene            2164..2364
                     /locus_tag="AC16BC_02490"
     CDS             2164..2364
                     /locus_tag="AC16BC_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR3240513.1"
                     /translation="MSPVVCPRCGRVNDTRSSFCEGCLHEFPSSIWDARTLICPECRY
                     ENSYSSDHCERCHEPLKPGQAE"
     gene            complement(2376..2798)
                     /locus_tag="AC16BC_02495"
     CDS             complement(2376..2798)
                     /locus_tag="AC16BC_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HIR3240514.1"
                     /translation="MLFDRDNQWRFTTIAQKRRQDAPLEGEDELIAQAMDLHPELDPI
                     WELGEMSATPQEVNGTVVNPFIHTALHVLVEKQLQTKSPPETMETLNRLTASGIERHE
                     AVHAIASVYAELYFTTFRRGQQFEELTYVELLKQLDAE"
     gene            complement(2810..3094)
                     /locus_tag="AC16BC_02500"
     CDS             complement(2810..3094)
                     /locus_tag="AC16BC_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240515.1"
                     /translation="MDPIELNDPEAIEELLGKISLHGRGFQTDCLLADVYDAGLTYPD
                     FLKAHGTDPEAKYGDQSPAWANYHVRQGKKVFMVYGGDGGVRRTHTTDTP"
     gene            3374..4093
                     /locus_tag="AC16BC_02505"
     CDS             3374..4093
                     /locus_tag="AC16BC_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240516.1"
                     /translation="MTGVGSRHALAVVAAFKRRGWLVVGVDAELHPRHVNQFYLVSRG
                     DLPGFADEMLMLVRRERTTLVIPTCDVDCLAVSRRSAEFRRLGAGAYLPDPATVEALT
                     GSGGAVSALRRSGLQACDPLGFPSPVIPGVQWFEVSLCRDARLPASAVTCSVHELAAA
                     DGSAEVHAERCKGEVDVEELAARSARALSLPGPATLLIRREVDGRLGVVRVMLRPCEY
                     APLTDDVLDALLSLWERDQPP"
     gene            complement(4101..>4634)
                     /locus_tag="AC16BC_02510"
     CDS             complement(4101..>4634)
                     /locus_tag="AC16BC_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3240517.1"
                     /translation="SVRASVPPPLEPGVEYPYRIRGVDGSGETGDVPVVSVEPSDDGR
                     ARDLSWTPPAASAATSQRLYRGDAPELIATFQDLTTSGYRDKGIAPYPVPTGLTITPE
                     DAAHLVTWSPVLNASWGYRLYWWEQAVNGAVSTGSEDVVDTQYRRTGLHAGSIYRYAV
                     QVVGNPGVSPASSVTAP"
BASE COUNT          872 a         1319 c         1549 g          894 t
ORIGIN      
        1 cgcgtgttcg gcggcatgtc gatgccgacc gagttgattc tggagtatct gtacaagtcg
       61 ccgaacgcgg atcgcatgac gaccaaaccc ctgcaggtat agcgcatcgg gtagccaccg
      121 aacaggagga tagacatgtc gcttgagaca ttaaagattt cacccggctt tgagtcgtac
      181 atgccgaagg aatacgtgga cctggtcgaa cgcggaccgt acggccgcga ggtcaaggtc
      241 agcgagatgg ggacgttcaa agaagtcctt gaagagcacc ccatgtgcgc gggttgcgcc
      301 atgacgttgt tcatccggac cgcgctcatc gggttgccgg ctccggaaca caccgtgatt
      361 ctgggcacgg ccgggtgcgg ccgccttgcg ctgtcgcagg cgtcgatccc gttcatctac
      421 ggcaactacg gcgataccaa cggggtggcg tccgggttga agcggggctt ggagatccgc
      481 ttcccgaatc agcagaagga cgtggtggtt atggccggcg acggcggact ctccgatatc
      541 gggttcagct gcgtcatgca ttcctggttc cgcaaggaaa agttcaccac cgtgatgctg
      601 gacaacgagg tgtacggcaa caccggcggc caggagagcg ggatgaccaa caagggtgtc
      661 gtcctcaaga tggcgccgca cggcaagaag ttcgagaaga tggacatgat cggtctcgcg
      721 aagaccgcgg gtgtggcgta catcgcgcgg ctcgccccga cgaatccgac ccggatcgcg
      781 aacgtgatcc gcaaggcggt gttgatcgcg cgcgaagtgg ggccgaccta cgtgcaggcc
      841 tacacgtcgt gcaacatcga atacgcgatc ccgaccgcga aagtgatgga cgacgcgcgc
      901 gacgtggaga aggatcgcta cggtttcatg gagatcatct cggacgacgc caaggcctat
      961 ctggagcgga tcgagcagga aaagaaggcc aagaaggcgg tatcggcgcc gaaggcctag
     1021 tggtcggacg gcagggtaac ggacgggagg agcgggcgtc atgaagcgat ataacatccg
     1081 gatggccggc atcggcgggc agggcgtggt gacggcctcg cacgtgatga gcaacggggt
     1141 gatcatcgcc gggggcgaaa gcaccctcgt gcccttcttc ggatcggaaa agcgcatggc
     1201 tccggtggag agctacgtgc gggtggccga aggacgcatc tacgagatcg gggagatcat
     1261 ctacccgaac gtcatcatga tcttccacac ccaggtgatc acgcacggca agtcgtacac
     1321 gatgccgttt tattcggggt tgaaggacga cggcgtcgtg ttgatcaact ccgagcatcc
     1381 gctgacgctc gagccggacc aggaacgaga gcttcgtgaa aaacgggcca aggtgttgta
     1441 tatccccgcc agcaggttgg cgtccgaaat cggcggaacc gagctggcga ccaatatggg
     1501 catggttggc gcggtggccg ggaccatcgg tttacccgac ctggcgtctc ttgagcagtc
     1561 cgtcaaggaa cgctttctcg ggaagggctt tgtggtgtct ggcggcacgg cgtcactcga
     1621 taacgtgatc gagaagaaat tcgggaagaa ggtggaattg attcaaaaaa atatggacgt
     1681 gatcaaggcg gcgcacaaat acgcgttgga tcacaaatgg aacgaactca cggcgactca
     1741 ggcggctgcg gccaacacct tggccgcggc cgctcgttag tccgccatca cgttgacaca
     1801 ggggtgattc atgtacgcag tggcgcaaat cgatgtggat atctgctcga agacgagctg
     1861 ccggctctgc acccagttct gtccggagtc caatacgatc aattacgacg tcaagcgcaa
     1921 caccgcctac gttgcggtgg atcgctgcaa agggtgcgag atctgcgtgg gcatctgcga
     1981 caacctcgcc aagcaccacg cgatcaagat ggtgccgatt accgacgtgg tcggcgggtc
     2041 tgaaatctcc aagctcggct tccacaagtt ttagtgaacg atcacgcggg tgagtccggc
     2101 cgggttcccg acaaggggcc cggccgattt gtttcactcc ccatcggtca aaggatgtcc
     2161 gatatgtcgc ccgtcgtgtg tccgcgctgc ggccgcgtga acgacacgcg aagcagcttc
     2221 tgcgaagggt gtttacacga attcccgtca tccatctggg atgcgcgaac gctgatctgt
     2281 cccgagtgcc gctacgagaa ttcgtattcg agcgaccact gcgagcggtg tcacgaaccg
     2341 ctcaagcccg gacaagccga gtaacggcgg ttgagttact cggcgtcgag ctgtttgagc
     2401 agctcgacgt aggtgagttc ttcgaactgc tgcccgcgac ggaacgtcgt gaaatagagt
     2461 tcggcgtaca ccgaggcgat ggcatggacc gcctcgtggc gctcaatccc gcttgcggtc
     2521 agccgattca gcgtttccat ggtttcgggt ggggacttgg tctgcagttg tttttccacg
     2581 aggacgtgca gcgcggtgtg gatgaacggg ttgactaccg tgccgttgac ctcctgcggc
     2641 gtcgcggaca tctcgcccaa ttcccagatc ggatcgagtt caggatgcag gtccatggct
     2701 tgggcgatca gttcgtcttc accctcgagc ggggcgtctt gacgacgttt ctgggcgatg
     2761 gtcgtaaatc gccattggtt gtctcgatcg aacagcacgc ggttctctcc tagggggtgt
     2821 cggtggtgtg ggtccgccgg acgccgccgt ctccgccgta gaccatgaag accttcttgc
     2881 cctggcgcac gtgataattc gcccaggcgg ggctttgatc gccgtatttc gcctcgggat
     2941 cagtcccatg ggctttaaga aaatccgggt aggtgaggcc ggcgtcgtac acatccgcga
     3001 gcaaacagtc ggtctgaaag ccgcggccgt gaagcgagat tttcccgagg agctcttcga
     3061 tggcttcggg gtcgttcaat tcaatggggt ccattgtaca ctcttgagga tccagtgcgc
     3121 gcccgtgttt catacatgca attttacggg accgatggga tggtaagcaa gatcgccgga
     3181 atcctggcgc aatgtcgcgc agcgaggccc gaaatccggc aatcaatagc tcatgaccgg
     3241 ctataccgca atgggcctgg cacaactctt gaagatccta cgccgggcga cgtgcgttgc
     3301 cgtcgggtac gtcatgggcg cacgtcaatg aggcgcggtg tgggtgatta ggcgtttgat
     3361 caagagagtg gtcgtgaccg gagtcggatc gcgccacgcg cttgccgtgg tcgcggcgtt
     3421 caaacggcgg ggctggttgg tggtcggcgt cgatgccgag ctccatcccc gccacgtcaa
     3481 tcagttttat ctggtgtcgc gtggagacct ccccggattc gccgatgaga tgctcatgtt
     3541 ggttcggcgc gaacgcacca cgctggtcat tccgacctgc gacgttgact gcctggccgt
     3601 ctcgcgacgc tcggccgaat tccgtcgttt gggagccggc gcgtatctgc ctgatcccgc
     3661 gaccgtcgag gcattgaccg gctccggagg cgcggtctcc gcccttcggc gttcaggctt
     3721 gcaggcgtgc gatccgctcg gtttcccctc gccggtgata cccggcgtgc agtggttcga
     3781 ggtgtcgctg tgtcgggacg cgcgtctgcc tgcttcggcc gtgacgtgct ccgttcatga
     3841 actcgccgcc gcggatggat ccgctgaggt gcatgccgag cgctgcaagg gggaagtgga
     3901 cgttgaagaa ctggccgcgc gctcggcccg cgcactcagc ctgcctggcc cggccacgtt
     3961 gttgattcgt cgggaggtgg acggccgtct gggcgtggtg cgggtgatgt tgcggccgtg
     4021 cgagtatgcg ccgttgaccg acgacgtgtt ggacgccttg ctgtcgctct gggagcggga
     4081 tcagccgccg taaacagcgg ttacggggcc gtgaccgacg aggcgggcga cacgccggga
     4141 ttccccacga cctgcacggc gtacctgtag atggaacccg cgtgaaggcc cgtgcgtcgg
     4201 tactgcgtgt cgaccacgtc ttctgatccg gtcgaaacgg ctccattcac ggcttgctcc
     4261 caccaataca ggcggtaccc ccaggacgcg ttgagcaccg gactccaggt cacaaggtgg
     4321 gccgcgtctt ctggggtgat cgtcagcccg gtgggcacgg gatagggggc gatccccttg
     4381 tcgcgatagc cggatgttgt gaggtcctgg aacgtggcga tcaattcggg cgcatcgccg
     4441 cggtagaggc gttgcgacgt ggccgcgctc gcggcgggcg gagtccacga gaggtcgcgc
     4501 gccctgccat cgtcagacgg ctcgacgctc acgaccggga cgtctccggt ctcgccggag
     4561 ccatcaacac cacgaatccg ataagggtac tccacgccgg gctcaagggg gggagggacc
     4621 gacgcgcgga cgct
//
LOCUS       DBNGTT010000108         7393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1427177, whole genome shotgun sequence.
ACCESSION   DBNGTT010000108 DBNGTT010000000
VERSION     DBNGTT010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1427177"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /gene="aspS"
                     /locus_tag="AC16BC_02515"
     CDS             complement(<1..1707)
                     /gene="aspS"
                     /locus_tag="AC16BC_02515"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR3240518.1"
                     /translation="MKRTHYCGDLRAQHAGATVTLVGWVHRRRDHGGLAFIDLRDRSG
                     LVQIVVTPDTEGGTAVAHEVRSEFVLEVTGQVIARPSGTENTSLPTGQVEVSVKRLSI
                     LNEAKTPPFQIDAPGDVAETVRLKYRYLDLRRPEIQQHFLVRHRVTSLVRAFLNQHGF
                     LDVETPVLTKSTPEGARDYLVPSRVNPGQFFALPQSPQLFKQLLMVAGFDRYYQIVKC
                     FRDEDLRADRQPEFTQIDIEMSFIDREDVLALMEEMIASLFAEIKGVTLPRPFPRLSY
                     AEAMTRYGVDKPDTRFGVELRDVSGIVKASAFQVFRQTLDKGGIVKGINARGQAHWSR
                     KDLDDVTNEAKALGAKGLAWLKVTDSGVESPIAKFFDDATLSAVVRALDGKPGDLLLF
                     VADAPATTHAVLGALRLSLGKRLNLIDESKFNVLWVLDFPLLEFDAEEKRWVAMHHPF
                     TAPMDEDVPKLESDPGSARAKAYDMVLNGSEIGGGSIRIHRQDVQSRMFSLLGIGKDD
                     AEAKFGFLLEALQYGAPPHGGIAFGLDRLVMLLVGAESIRDVIAFPKTQKAVCPMTNA
                     PSP"
     gene            complement(1712..2512)
                     /locus_tag="AC16BC_02520"
     CDS             complement(1712..2512)
                     /locus_tag="AC16BC_02520"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3240519.1"
                     /translation="MSEERLQKLIARAGIASRRASEQLILDGEVTVNGRVVTELGSKA
                     DPDKDHIKVRGKLLQFKGPKVYAMLHKPKGYVTTMSDPEGRPTIADLIRGIRGRVFPV
                     GRLDFDTEGLLLLTNDGELANALMHPSHEVPKVYAVKIKGKAKDEEIAKLEAGGIYIG
                     PGRSAPCRVRRMRQAEQNEWLEITLHEGKKRQIRVMLDRLDHSVLKLIRTHYAGIPLG
                     DLPRGETRLLAPGEVKRLQTLALAPAKPQKREGPPRRPATRRVPAGAR"
     gene            complement(2739..2945)
                     /gene="iscX"
                     /locus_tag="AC16BC_02525"
     CDS             complement(2739..2945)
                     /gene="iscX"
                     /locus_tag="AC16BC_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HIR3240520.1"
                     /translation="MKLTWNDTEDIAIALADKFPGLDPLTVRFTDLHKWVIALPDFAD
                     DPAKSNEGILEAIQMAWLEESKNS"
     gene            complement(3011..3349)
                     /locus_tag="AC16BC_02530"
     CDS             complement(3011..3349)
                     /locus_tag="AC16BC_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3240521.1"
                     /translation="MTSPQSTDHATARAAITKHRVTFLPANLTVEVDEGTSILDAALN
                     HAIDLEHNCGGNCACSTCHVIVREGMEHLSERTEEEEDQLDDADGLTTHSRLGCQAKI
                     SGPVVVVIPD"
     gene            complement(3346..5187)
                     /gene="hscA"
                     /locus_tag="AC16BC_02535"
     CDS             complement(3346..5187)
                     /gene="hscA"
                     /locus_tag="AC16BC_02535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HIR3240522.1"
                     /translation="MGRIVGIDLGTTNSLVAYMDHGRPRIIADASDARLVPSVVSIYG
                     DGRVEVGVSAKRRLLTRPQETIYSIKRFMGKGWSDVQAEAASLPYRVSTDPGHRDVAR
                     VMAGGRAWTPPEISAFILRELKTQAEAFLGEPVTQAVITVPAYFNDSQRQATKDAGRL
                     AGLEVLRIVNEPTAASLAYGLQQRKQGVIAVYDLGGGTFDVSILKLKDGIFEVLATNG
                     DTHLGGDDFDHRVAAVLADDIKRRHGVGVAGSPELMQEVRVAAEAAKCRLSELDRTVI
                     EVPLPGGVTFSREITREEFEGMTGDLVAATLEPCRRALADAKVSPEQIDEVVLVGGST
                     RVPLVRRRVQELFGKVPHSELNPDEVVALGAAVQADILAGGIRDMLLLDVTPLSLGIE
                     TMGGIMSVLIPRNITIPTSAKETFTTSVDGQTAVAIHVLQGERELVKDNRSLARFELR
                     GVDPMPAGLPRVEVTFLIDANGILQVSATELRTGKFQSIEVKPSYGLTEAQIEQMLVD
                     SFKHAKSDLEQRALIEAKNEANEIFNASERALQRGRHLLETGEAERIEAAMAALKAAM
                     AGTDRTTIKKSKIALDEVTRHLAEVLINATMEEALRDRNVSEILPKV"
     gene            complement(5187..5792)
                     /locus_tag="AC16BC_02540"
     CDS             complement(5187..5792)
                     /locus_tag="AC16BC_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR3240523.1"
                     /translation="MPETPTGAVSPQPDYFAVFGLPRHLSIDAAALEAAYHRLSREVH
                     PDFHLTKPADERERLLARSALVNQAYRTLRDFHARVEYLVELEGGDALKPKAPPELLE
                     DVLEVQDLLEECRAQGRLGTDDPLTGRLSAEAARLQRALAALDAELVERSKAWDAAIG
                     ANGAGSPARGEVLARLQGLLSQRVYVVNLLRDINGVRTQGA"
     gene            complement(5836..6207)
                     /locus_tag="AC16BC_02545"
     CDS             complement(5836..6207)
                     /locus_tag="AC16BC_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR3240524.1"
                     /translation="MEISLNMVDEPKVEQPALKVTPEAAKEAKRLLGLQGTPDMVVRI
                     SVQGGGCSGFTYQMKFDTKVSEFDEVIQTDDAKFVVDAKSALFLKGTTLDYVNALMGG
                     GFKFVNPNATSTCGCGESFSA"
     gene            complement(6260..6667)
                     /gene="iscU"
                     /locus_tag="AC16BC_02550"
     CDS             complement(6260..6667)
                     /gene="iscU"
                     /locus_tag="AC16BC_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HIR3240525.1"
                     /translation="MAYSDKVVDHYNNPRNVGSLDKSDPSVGTGVVGAPECGDVMKLQ
                     IKVNQQGVIEDAKFKTFGCGSAIASSSLATEWLKGKTIDEASQIKNTDIVQELALPPV
                     KIHCSVLAEDAIKAALADVKKKQESLTSQGAGS"
     gene            complement(6695..>7393)
                     /locus_tag="AC16BC_02555"
     CDS             complement(6695..>7393)
                     /locus_tag="AC16BC_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3240526.1"
                     /translation="VIFHCDATQGVGKIPVDVQAMGIDLMSFSSHKIYGPKGVGALYV
                     RRRNPRVRLAAQMDGGGHERGMRSGTLPVPLIVGFGEACRIAGEVMPTESVRLAALRD
                     RLRDGIFKGLDEVYVNGHQTKRLPHNLNVSFAYVEGEAMLMGLKDIALSSGSACTSAT
                     LEPSYVLRSLGVGSDLAHSSIRFGLGRFNTQEEIDFVIDRVVQAVTKLREMSPLYEMA
                     KEGIDLKAVNWSAH"
BASE COUNT         1226 a         2552 c         2227 g         1388 t
ORIGIN      
        1 cgggctcggc gcgttggtca tggggcagac cgccttttgg gtcttgggga acgcgatcac
       61 gtcgcggatc gactcggcgc cgaccagcag catgacgagg cgatccagac cgaacgcgat
      121 cccgccgtgc ggcggcgcgc cgtactggag cgcttccaac aagaagccga acttggcctc
      181 cgcgtcgtcc ttgccgatcc cgagcagcga gaacatgcgc gactgcacgt cctgccgatg
      241 gatgcggatg ctgccgccgc cgatctccga gccgttgagc accatgtcgt aggccttggc
      301 gcgcgcgctg ccggggtcgg attcgagctt cggcacgtct tcgtccatgg gcgcggtgaa
      361 ggggtggtgc atcgcgaccc agcgcttctc ctccgcgtcg aactccaaca acggaaagtc
      421 caacacccag aggacgttga acttcgactc gtcgatcaga ttgagtcgct tgcccagcga
      481 caggcgcaac gcgcccaaca ccgcatgcgt ggtcgcgggc gcgtccgcca cgaacagcag
      541 gagatcgccc ggcttcccgt ccagcgcgcg gaccacggcg ctcagcgtgg cgtcgtcgaa
      601 aaacttggcg atcggagatt cgacgcccga gtccgtcacc ttgagccatg ccagcccctt
      661 ggcgccgagc gccttggcct cgttggtgac gtcgtccagg tccttgcgcg accagtgcgc
      721 ctggccccgg gcattgatcc ccttgacgat cccgcccttg tccagggttt gtcgaaacac
      781 ctggaacgcg gaggccttca cgatccccga tacgtcgcgc agctccacgc cgaaccgcgt
      841 gtcgggcttg tccacgccgt agcgcgtcat ggcctcggcg taggacagcc ggggaaacgg
      901 ccgcggcaac gtgacgccct tgatctccgc gaacagcgac gcgatcatct cttccatcaa
      961 cgccaacacg tcttcgcgat cgatgaagga catctcgata tcgatctggg tgaactcggg
     1021 ctggcgatcc gcacgcaggt cctcgtcgcg gaaacatttc acgatctggt agtagcgatc
     1081 aaagcccgcc accatcagca attgcttgaa gagctgcggc gactgcggca gcgcgaaaaa
     1141 ctggccggga ttcacccggc tcggcaccag atagtcccgc gcgccctcgg gcgtgctctt
     1201 ggtcagcacc ggcgtctcca cgtcgaggaa cccgtgctga ttgaggaacg cgcgcaccag
     1261 gctcgtcacc cggtggcgca ccaggaagtg ttgctggatc tcgggccgcc gcagatccag
     1321 atagcgatac ttgaggcgca cggtctcggc cacgtctccg ggcgcgtcga tctgaaacgg
     1381 cggggtcttg gcttcgttca gaatcgacaa ccgcttgacc gacacttcca cctgccccgt
     1441 gggcaggctc gtattttcgg ttccggacgg acgcgcaatg acctggcccg tgacttccaa
     1501 tacgaactcg ctccgcacct cgtgggccac cgcggtgccg ccctcggtgt cgggcgtgac
     1561 cacgatctgg accaggccgc tgcgatcgcg caggtcgata aacgccaacc cgccgtggtc
     1621 gcgccggcgg tgcacccagc cgaccaacgt gacggtcgcg cccgcgtgtt gcgcccgcaa
     1681 gtcaccgcaa tagtgcgtgc gcttcatgcg gtcaccgcgc ccccgcggga acgcgacgag
     1741 tcgccggccg tcggggcggt ccctctcgtt tctggggctt ggcgggcgcc agggccaagg
     1801 tctgaagccg tttgacctct cccggcgcca acaaccgcgt ctcgccgcgc ggcaagtccc
     1861 cgagcgggat acccgcgtag tgcgtgcgga tcaacttcaa caccgaatga tcgaggcggt
     1921 ccaacatgac ccgaatctgt cgcttcttgc cttcgtgcaa ggtaatctcc aaccattcgt
     1981 tctgttcggc ctggcgcatc cggcgcacgc ggcagggcgc ggatcgcccc ggaccgatgt
     2041 agattccccc cgcttccagt ttggcgatct cctcgtcctt ggccttgcct ttgatcttca
     2101 ccgcatacac cttgggcacc tcgtggcttg ggtgcatcag ggcattggcc agctccccgt
     2161 cattggtcag cagcaacagg ccctcggtgt caaaatccag tcgccccacc ggaaacaccc
     2221 ggccgcgaat gccccggatc aagtcggcga tggtgggtcg gccctcggga tcgctcatcg
     2281 tcgtgacgta ccccttgggc ttgtgcagca tggcgtagac cttcgggccc ttgaactgca
     2341 acagcttgcc gcggactttg atgtgatcct tgtccggatc agccttactg cccaattcgg
     2401 tcaccacgcg gccgttgacc gtgacctcgc cgtcgaggat cagttgttcg gacgcgcggc
     2461 gagacgcgat accggcgcga gcgatcagct tttgcaaacg ttcttcggac atgggatcgg
     2521 gtgacgtcac gcgccgaagc gcagaggcgt ggcgaagacg tgcgcggatt gtgtcacagg
     2581 ccagcgggca gtgtcaacga cggacgcgct cagggcgcgg cgccgatgat cgcgatcgca
     2641 ggcggcccgc caccggctct ggtccgccat gggctccgca ggtacatgtt gagtgaggcc
     2701 ttacgagtgt tgaggcgcga ctacgttgcg aggtcgctct aactattctt ggactcctcg
     2761 agccacgcca tctggatcgc ctccagaatg ccttcattgg acttggccgg atcgtcggca
     2821 aaatcgggca gcgcgatcac ccacttgtgc aggtcggtga accgcacggt cagcgggtcg
     2881 aggccgggaa atttgtcggc caacgcgatg gcgatgtctt cggtgtcgtt ccaggtcagc
     2941 ttcacgcgac tccctcaaat cccccctgcc cccccctttc acaaaggggg atggggggat
     3001 ttctcaattg ttaatccgga atcacgacga caaccgggcc cgaaatcttc gcctggcatc
     3061 ccagccggga gtgcgtggtc aggccgtccg cgtcgtcgag ctggtcttct tcttcctcgg
     3121 tacgttccga caagtgctcc atcccctccc gcacgatgac gtggcaggtg gaacacgcgc
     3181 agttgccgcc gcaattgtgt tcaaggtcga tcgcgtgatt gagcgccgca tccaggatcg
     3241 acgtgccttc gtcgacctcc acggtcaggt tcgcgggcag aaacgtgacg cggtgtttgg
     3301 tgatcgccgc gcgagccgtg gcgtggtcgg tggattgggg actggtcata ccttgggcag
     3361 gatttccgag acgttgcggt cgcgcaaggc ttcttccatg gtcgcgttga tcagcacttc
     3421 ggccagatgc cgcgtgacct cgtcgagcgc gatcttgctc ttcttgatcg tggtgcggtc
     3481 agtccccgcc atcgcggcct tcagcgcggc catggcggcc tcgatacgct cggcctctcc
     3541 ggtctccaac aggtggcgcc cgcgctggag cgcccgttcg gacgcgttga agatctcgtt
     3601 ggcctcgttc ttggcctcga tgagcgcccg ttgctccaag tcgctcttgg cgtgtttgaa
     3661 cgagtccacc agcatctgtt cgatctgcgc ctcggtcagc ccgtacgacg gtttgacttc
     3721 gatcgactgg aactttccgg tccgcaattc ggtggcgctg acctggagga tgccgttggc
     3781 gtcgatcagg aacgtgacct ccacccgcgg caagccggcc ggcatggggt ccaccccgcg
     3841 caactcgaag cgcgccaggc tgcggttgtc cttgaccagt tcccgttcgc cttgcagcac
     3901 gtggatggcg accgcggttt gcccgtcgac cgacgtggta aacgtctctt tggcgctggt
     3961 cgggatcgtg atgttgcgcg ggatgagcac gctcatgatc ccgcccatgg tctcgatgcc
     4021 cagcgagagc ggcgtgacgt ccagcagcaa catgtcgcgg atcccgccgg ccagaatatc
     4081 ggcctggacc gccgcgccca acgccacgac ttcgtcgggg ttcaattccg aatgcggcac
     4141 ttttccgaag agttcctgga cccgccggcg gaccaacggc acgcgcgtgg accccccgac
     4201 caggaccact tcgtcgatct gttccggcga gaccttggca tcggcgagcg cgcgccggca
     4261 cggctccagc gtcgccgcga ccaaatcacc cgtcatgccc tcgaactcct cgcgggtgat
     4321 ctcccgggag aacgtgaccc cgccgggcaa cggcacctcg atcaccgtgc ggtcgagctc
     4381 ggacagccgg cacttggcgg cttcggccgc gacgcggacc tcttgcatca gctccggcga
     4441 ccccgcgacc cccactccgt gccggcgttt gatgtcgtcg gccagcaccg cggccacccg
     4501 gtggtcgaag tcgtcgccac caaggtgggt gtcgccgttg gtggccagga cttcgaagat
     4561 cccgtccttg agtttgagga tcgacacgtc gaacgtgccg ccgcccaaat cgtacaccgc
     4621 gatcacgccc tgcttgcgct gctggaggcc gtaggccagc gaggcggcgg tcggctcgtt
     4681 cacgatccgc agcacctcga gcccggcgag ccgtcccgcg tccttggtgg cctgccgttg
     4741 cgagtcatta aaataggcgg gcaccgtgat cacggcctgc gtgacgggtt cgcccagaaa
     4801 ggcttcggcc tgggtcttga gttcgcgcag aatgaacgcg gaaatttccg gcggggtcca
     4861 cgcccggccg cccgccatga cgcgcgccac gtcccggtgt ccgggatccg tcgacacccg
     4921 gtacggcagc gacgcggcct cggcctggac gtcggaccac cccttgccca tgaaccgttt
     4981 gatcgaataa atcgtctctt gcggacgtgt cagcagacgg cgtttggcgg acacgccgac
     5041 ctccacgcgt ccgtccccgt agatcgagac caccgacggg accaggcgcg cgtcggacgc
     5101 gtccgcgata atgcggggcc ggccgtggtc catgtacgcg accaagctgt tggtcgtccc
     5161 caggtcaatg cccacgatcc gtcccatcac gccccctgcg tcctgacccc gttgatgtcc
     5221 cgaagcaaat tcaccacata cacccgctgc gagagcaggc cttgcagacg cgcgagcacc
     5281 tcgccgcgcg ccgggctccc cgccccgttc gcgccgattg ccgcgtccca ggcctttgac
     5341 cgttcgacca actccgcgtc cagcgcggcc agcgcccgct gcaaacgggc ggcctccgcg
     5401 ctcagtcgac cggtcaaggg atcgtccgtt cccaaccgcc cctgcgcccg gcactcctcc
     5461 agcaggtcct gcacttcgag cacgtcttcc aacaattccg gcggcgcctt gggtttgagc
     5521 gcgtcgccgc cctccagttc gaccagatac tccacgcgcg cgtgaaaatc cctgagcgtg
     5581 cggtaggcct gattcacgag cgcggagcgc gccaacaggc gctcgcgttc gtcggccggt
     5641 ttggtcaggt gaaagtcggg atgaacttcg cggctcaacc gatggtacgc cgcctcgagc
     5701 gccgcggcgt cgatgctcag atggcgcggc aatccaaaca ccgcaaagta atccggctgc
     5761 gggctcaccg cgcccgtcgg cgtttcaggc atggtcgcgt ccctaccttt ggtcagacgg
     5821 cctgccctgc cggactcacg ccgagaagga ctccccgcac ccgcacgtgc tggtcgcgtt
     5881 gggattgacg aacttgaacc cgccgcccat cagcgcgttc acgtaatcca acgtcgtgcc
     5941 cttgagaaac aacgcgctct tcgcgtccac gacgaatttc gcatcatcag tctgaattac
     6001 ttcatcgaat tcagatactt tcgtatcaaa tttcatctga tacgtgaacc cagagcaccc
     6061 gccgccctgc accgagatac gcaccaccat atcgggcgtg ccctgcaacc ccagcaggcg
     6121 cttggcttcc ttcgccgcct ccggcgtcac cttcagggcg ggctgttcga ccttgggctc
     6181 atccaccatg ttcaacgaga tttccattgt ctcctctcca tgctccgagt gcgaaagccg
     6241 gtctacgccg ccacgtcggt tatgatcccg caccttgcga cgtgagcgat tcttgtttct
     6301 tcttcacgtc ggccagcgcg gccttgatcg cgtcttcggc cagcaccgag cagtggatct
     6361 tgaccggcgg cagggccaat tcctggacga tatccgtgtt cttgatctgc gaggcctcgt
     6421 cgatggtctt gcctttgagc cactcggtcg cgagactcga gctggcgatc gcgctgccgc
     6481 agccgaaggt cttgaacttg gcgtcttcga tcacgccctg ctgattgacc ttgatctgca
     6541 gcttcatgac gtcgccgcat tccggcgcgc ccacgacccc ggtgcccacg ctgggatcgc
     6601 tcttgtccag actccccacg ttccgcgggt tgttgtagtg atcaacgacc ttatcgctgt
     6661 atgccatgac gcctccccct tgcgcgcgct cgtgttaatg agccgaccag ttcaccgctt
     6721 tcagatcaat cccctctttc gccatttcat acaacggcga catctcacgc aacttcgtca
     6781 ccgcctgtac cacgcggtcg atcacaaagt cgatttcctc ttgcgtgttg aaccgcccca
     6841 gcccgaaccg gatcgacgag tgcgccaagt ccgatcccac cccgagcgac cgcagcacgt
     6901 aggacggctc caacgtcgcg gaggtgcatg cggaaccgga cgacaacgcg atgtccttca
     6961 ggcccatcaa catggcttcg ccctcgacgt acgcgaaact gacattgagg ttgtggggca
     7021 accgcttggt ctggtggccg ttcacataga cttcatccaa acctttaaag attccgtcgc
     7081 gcagccgatc gcgcaacgcc gccagacgaa ccgactcggt cggcatcacc tcgcccgcga
     7141 tccggcaggc ttcgccgaac ccgacgatca gcggcaccgg caacgtgccg gaccgcatgc
     7201 cgcgctcgtg cccgccgccg tccatctgcg cggccaatcg cacccgcggg ttgcggcgac
     7261 gcacgtacaa cgcgccgacg cccttgggac cgtagatctt gtgcgaggag aacgacatga
     7321 ggtcgatccc catcgcctgc acgtccaccg gaatcttgcc cacgccctgg gtcgcgtcgc
     7381 agtgaaagat cac
//
LOCUS       DBNGTT010000109         2562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_661591, whole genome shotgun sequence.
ACCESSION   DBNGTT010000109 DBNGTT010000000
VERSION     DBNGTT010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_661591"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /gene="hemG"
                     /locus_tag="AC16BC_02560"
     CDS             complement(<1..1303)
                     /gene="hemG"
                     /locus_tag="AC16BC_02560"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIR3240527.1"
                     /translation="MAAVRVAVIGGGISGLGVAYALAREGVPFRLIEAAPSVGGMIRS
                     ERVDGFLIEHGPNSALNASVDVEAVITDLGLLPDRVFAAPASRKRYIVKHEQLVPLPM
                     GLIEFLRTPLWSTRAKARLLKEPFAAPPPAGEESIASFVSRRLGREFLEYAVDPFVSG
                     VYAGDPERLSVQATFPKLAALEREHGGLVRGAIAGIGKSRAPRPSQRGIYSFREGLSQ
                     LPVAISARLGESLWCHARVAALTRRVDGFRVDVERHRERVTLDADRVVIATPADVAAA
                     LVRPLASHLADELAAIEYAPVAVVATAFARTAVQHPLDGFGCLVPRSERRTLLGSLWN
                     SSLFPGRAPAGAVLLSNFVGGARHADLVDRPDDELTRLVVEDLGRLLGVNGPPRWTRI
                     ARYRRAIPQYLLGHASRVARIEHATAGIPGLSLVGNYLRGVA"
     gene            complement(1309..2265)
                     /gene="hemH"
                     /locus_tag="AC16BC_02565"
     CDS             complement(1309..2265)
                     /gene="hemH"
                     /locus_tag="AC16BC_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3240528.1"
                     /translation="MLFNLGGPDTLDDVKPFLQNLFSDPEVIPLPPPFRQILAWWIAT
                     RRAPTAKGYYEKIGGGSPLRRLTADQAAALEKALSGRGRHKVVVAMRYWKPDTEDAVT
                     ELAAARPSRIVLLPLYPQYSFATTRSSAEYFRRVWGQRGLDGVPIVSIDQWHTHAGYL
                     DAMAEQISATRAAMPNGDGSPVHLVYSAHGLPMKLIEQGDPYQRQVEEQVRILTGRLP
                     EWASDPARVHLGYQSRVGRDKWLEPSMEELIHRLGSEGATRVLVVPVSFVSDHSETLY
                     EIDIQYRQLAAESGIRVFARSASLNDSPTFIRALADLVASAA"
     gene            complement(2298..>2562)
                     /locus_tag="AC16BC_02570"
     CDS             complement(2298..>2562)
                     /locus_tag="AC16BC_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3240529.1"
                     /translation="VDWRIDLAEAKRRVGSRVALQGNLDPCVLYAQPDVIRRHARRVL
                     DAYGREPGHVFNLGHGILPDVSVAHAKSLVDAVHEESRALRSS"
BASE COUNT          446 a          898 c          855 g          363 t
ORIGIN      
        1 ccgccacgcc ccgcaagtag ttcccgacca gcgataagcc cgggataccc gcggtcgcgt
       61 gctcgatccg cgcgacgcgc gacgcgtggc cgagcaggta ctgcgggatc gcccggcggt
      121 agcgagcaat gcgcgtccat cggggcggcc cgttgacgcc gagcagccgg ccgaggtcct
      181 cgaccaccag acgcgtcagc tcgtcgtccg ggcgatcgac caggtctgcg tgccttgcac
      241 cgccgacgaa attcgacaac aacacggcgc ccgccggggc acgcccggga aagagcgacg
      301 agttccacag cgaccccaac agcgtgcgac gctccgaccg tgggaccaaa caaccgaacc
      361 cgtccagtgg atgttggacc gcggtgcggg caaacgcggt ggccacgacc gcgaccggag
      421 cgtattcgat cgcggccaac tcgtccgcca ggtgggacgc cagcgggcgc acgagagcgg
      481 ccgcaacgtc cgcgggcgtc gcgatgacca cgcggtcggc atcaagcgtg acccgctccc
      541 ggtgccgctc gacgtcaacg cggaacccgt ccacccgacg ggtcagcgcg gccacccgcg
      601 catgacacca gagcgactcg cccaaccgcg cgctgatcgc gactggaagt tggctcagcc
      661 cttcccgaaa cgagtagatg cctcgttggc tcgggcgcgg cgctcgggac ttgccgatcc
      721 cggcgatggc gccgcggacg agcccgccgt gctcgcgttc caacgccgcg agtttcggga
      781 aggtcgcctg cacggagagg cgctcggggt ccccggcata caccccggac acgaacgggt
      841 ccaccgcgta ctccagaaac tcgcgcccga gccggcgcga gacgaatgac gcgatcgatt
      901 cctcgcccgc tggcggcggc gcggcaaacg gctctttgag caggcgtgcc ttggcgcgag
      961 tgctccacaa cggcgtacga agaaactcga tcagccccat ggggagggga acgagctgtt
     1021 cgtgtttgac gatatatcgt tttcgcgagg caggcgcggc gaacacgcga tccgggagca
     1081 ggcccagatc agtgatcacg gcctcgacgt ctaccgacgc gttcagcgcc gaattcgggc
     1141 cgtgctcgat cagaaatcca tcgacccgct cgctgcggat catgccgccc acggatggag
     1201 cggcttcgat gagtctgaag ggaacgcctt cgcgggccaa cgcatacgcc acgccgagcc
     1261 cggaaatccc gccgccgatc accgccaccc ggaccgcggc catcggaatt aggccgcgga
     1321 cgcgacgagg tcggccaacg cgcggataaa ggttggcgag tcgttcagcg acgccgagcg
     1381 ggcgaacacg cggataccgg attcagccgc cagctgtcgg tattgaatgt cgatctcgta
     1441 cagggtttcg gagtggtcgg agacaaaact caccggcacc accaacacgc gcgtcgcgcc
     1501 ctcggagccg aggcgatgga tcagttcttc catggacggc tccaaccatt tgtcccgccc
     1561 cacgcggctc tgatacccca ggtgcacgcg tgcggggtcc gacgcccact ccgggagccg
     1621 gcccgtgagg atgcgcacct gctcctccac ctgccgctgg tagggatcgc cctgttcgat
     1681 caacttcatg ggcagcccgt gcgcggaata gaccaagtgc acgggcgatc cgtccccgtt
     1741 cggcatggcc gcgcgcgtcg cactgatctg ctccgccatg gcgtcgagat acccggcgtg
     1801 cgtgtgccat tggtcgatcg acacgatggg caccccgtcg aggccgcgtt gaccccacac
     1861 gcgccggaag tactcggcgc tggagcgcgt ggtggcgaac gaatactgcg ggtacagcgg
     1921 caagagcacg atccggctcg gccgcgccgc ggccagctcc gtcaccgcgt cttcggtgtc
     1981 gggtttccaa taccgcatgg ccaccaccac cttgtgccgg ccgcggccgg acaacgcctt
     2041 ttccagcgcc gcggcctggt ccgcggtcag gcgccgcaac ggcgacccgc cgccgatttt
     2101 ttcgtagtaa cccttggcgg tcggcgcgcg tcgcgtcgcg atccaccatg cgaggatctg
     2161 ccgaaacggc ggggggagcg ggatgacctc gggatcggag aacaggtttt gaagaaaggg
     2221 cttgacgtcg tcgagcgtgt cgggcccccc gaggttgaag agcacgaccc ccgtcagctg
     2281 agcggacggc aatccgctca agacgaccgc agcgcgcggc tttcctcgtg caccgcgtcg
     2341 accaggcttt tggcgtgcgc caccgacacg tcggggagaa tcccgtggcc gagattaaag
     2401 acgtggccgg gttcgcggcc gtacgcgtcg agcacccgcc gcgcgtggcg acggatcacg
     2461 tccggctgcg cgtagagcac gcacgggtcc aggttgcctt gcagcgcgac ccgcgagccc
     2521 acgcggcgct tggcctccgc gagatcgatc cgccaatcca cg
//
LOCUS       DBNGTT010000110         1563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_406417, whole genome shotgun sequence.
ACCESSION   DBNGTT010000110 DBNGTT010000000
VERSION     DBNGTT010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_406417"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..169
                     /locus_tag="AC16BC_02575"
     CDS             2..169
                     /locus_tag="AC16BC_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HIR3240530.1"
                     /translation="MAYATNDGITQELEYCRSLFTANRGWRVLDVTTRAVEEIAADIM
                     SALPSPTRPGS"
     gene            450..>1563
                     /locus_tag="AC16BC_02580"
     CDS             450..>1563
                     /locus_tag="AC16BC_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3240531.1"
                     /translation="MSQPEIESVLTERRVFKPKAAFSRAAHVKSLKEYDKLAKLAQKN
                     PEKFWSDLAKELYWFKRWKKVLEWKPPFAKWFVGGQTNVSVNCLDRHLHTWRRNKAAI
                     IWEGEPGDTRTLTYQDLHREVCRFAAVLKQLGVEKGDRVAIYMPMIPELPIAMLACAR
                     IGATHSVVFGGFSAEALKDRINDAQAKLVVTADGGYRKGAVVALKEQVDRAVEHCPSV
                     TKVVVCRRTNSAVTMKPERDVWWHDVMRDAPLRCEPEHLDSEHPLFILYTSGTTGKPK
                     GIVHTTAGYLLGAAVTTKWVFDLKDDDTYWCTADIGWVTGHSYIVYGPLANGATVVMF
                     EGVPNHPDASRFWQVVDKLGVTIFYTAPTAIRALIRH"
BASE COUNT          321 a          525 c          485 g          232 t
ORIGIN      
        1 catggcctac gccaccaacg acggcatcac acaagaactg gaatactgcc gatcattgtt
       61 cacggccaac cgcggctggc gcgtcctgga cgtgaccacg cgcgcggtcg aggaaatcgc
      121 cgcggacatc atgagcgcgc tgccctcccc cacgcgacca ggctcctgac tttcaagtaa
      181 cagagcaacg ccagatggag cctgttcagg gacggccaga tgcaaggcgg aggaaggacc
      241 ttgcgcgcgg agcgtactgg gtcgtacgtg agcacgcaac gccgaccgac aacgccgcag
      301 atggccgttc atggacaggc tcccaggact gattgacaag tcgagcgacc cttccctaca
      361 atgctcgcct caacgcgccg gccgcatgat tcgcgcgact cggcgcgaat tgcctcgcag
      421 acgccactga cctcgaagga gactccacga tgtcgcaacc cgagatcgag tccgttttaa
      481 ccgaacgccg cgtcttcaaa cccaaggcgg ccttcagccg ggcagcccac gtcaaaagcc
      541 tcaaggagta cgacaaactc gccaaactgg cgcagaagaa cccggaaaaa ttctggagcg
      601 atctggccaa ggagctctat tggttcaaac ggtggaaaaa ggtgctcgag tggaagccgc
      661 cgttcgccaa gtggttcgtg ggcggccaga ccaacgtgtc ggtcaattgc ctggaccggc
      721 acctgcacac atggcggcgc aacaaggccg cgatcatctg ggaaggcgaa cccggcgaca
      781 cccgcacgct cacctatcag gatttgcacc gcgaggtgtg ccggttcgcc gccgtgctca
      841 aacagttggg cgtggagaag ggcgaccggg tcgcgatcta tatgcccatg attccggaac
      901 tgcccatcgc catgctcgcg tgcgcccgga tcggcgccac gcacagcgtg gtgttcggcg
      961 gattctccgc ggaggcgctc aaggaccgca tcaacgacgc gcaggccaaa ctcgtggtca
     1021 cggccgacgg cgggtaccgc aaaggcgcgg tggtggcgct caaggaacag gtggaccgcg
     1081 cggtcgagca ctgcccgtcc gtcacgaagg tcgtggtctg ccgtcgcacc aactcggccg
     1141 tcacgatgaa gccggaacgc gacgtgtggt ggcacgacgt gatgcgcgac gcgccgctgc
     1201 gctgcgagcc cgagcacctg gactcggagc atccgctctt catcctgtac acgagcggca
     1261 ccacgggcaa acccaagggc atcgtgcaca ccaccgcggg gtacctgctc ggcgcggccg
     1321 tcaccaccaa gtgggtgttc gacctcaagg acgacgacac ctactggtgc accgcggaca
     1381 tcggctgggt cacgggccac agctacatcg tgtacgggcc gctcgccaac ggcgcgaccg
     1441 tggtgatgtt cgagggcgtg cccaaccacc ccgacgcctc gcgcttctgg caggtcgtgg
     1501 acaagctcgg cgtcaccatc ttctacaccg cgcccaccgc gatccgtgcg ctgatccgcc
     1561 acg
//
LOCUS       DBNGTT010000111         3874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2833710, whole genome shotgun sequence.
ACCESSION   DBNGTT010000111 DBNGTT010000000
VERSION     DBNGTT010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2833710"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /gene="secD"
                     /locus_tag="AC16BC_02585"
     CDS             <1..1559
                     /gene="secD"
                     /locus_tag="AC16BC_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR3240532.1"
                     /translation="TTPWFKSLPGWWQRYLPHKGITLGLDLQGGMHLVMEVDVEKAVD
                     NSLDRMALDLGDRLKDQQIAATAARQGRSVVVTMTDEADREKISKIVDERFGALTLGD
                     LKDKVLTLNLRSGEAERVQKAAVSQSIETIRNRIDQFGVTEPLIQLQGERQILIQLPG
                     EKDPQRALNVIGKTALLEFKLLDEDSPVLQQLPELVPAGQEQSWLAEWRAKIPPGDEI
                     LFERVAEEKGPVNKRPYLVKQRAMITGESLRDALVTIGDFNQAQVSITFDAAGSAQFE
                     RVTGENVKKRLAIVLDNSVYSAPVIQERIAGGRAQISGSFTMDEANDLAVVLRAGALP
                     APVNVIQNVTVGPSLGQDSIDAGIHAAWIGTLLVVAFMAIYYRLAGVIADFALALNVI
                     LLIGALAALNATLTLPGIAGIILTIGMSVDSNVLIFERIREELRQGKPVRLAVDAGYE
                     KAFLTIVDAHVTTLITAVVLFLFGTGPIKGFAVTLTLGVTINLFTALVGTKVVFDLMN
                     TRAKVARLSI"
     gene            1568..2488
                     /gene="secF"
                     /locus_tag="AC16BC_02590"
     CDS             1568..2488
                     /gene="secF"
                     /locus_tag="AC16BC_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR3240533.1"
                     /translation="MFELLGKTNIDFIGKRHYAFLLSGVLALLGVITVIQIARGQANT
                     GIDFAGGTAVQLQFEKPVQLDEARALLEREGIPDAELQQFTGGNKLLIRVKKQTTIQE
                     KTADRLVAVFKKGFESNPFVVDSTTEIGPTVGAKLQQDAVKAITIAMIGIIIYIALRF
                     EFRFGVAAALATFHDVLAVLGIMGLLGKEINLLIVTALLTLAGYSLTDTVVVFDRIRE
                     ILRTRRRDPLGQVINQGVNQVLSRTIVVSLTVFLVLLALFFFGGEVIHDFSLALLMGV
                     VIGTYSSIFVASPILMLWPGAQGKLLKRTA"
     gene            2664..3251
                     /locus_tag="AC16BC_02595"
     CDS             2664..3251
                     /locus_tag="AC16BC_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240534.1"
                     /translation="MLVLAALGACATRPPVFEPVPVGSRSGAHDVVVQIQPIDTQLGS
                     EDRRRYGVDFSAYFAAFRVSVDNRTAHAVAADLAPSTLGPSAGPITGVLSDEELVRIY
                     RRGGMDSDAVELVAKAPSVVKRELEQIRAARIQAARLEPGGRVEGVLFFRPPAGGECV
                     PSVLTIKGIAILDEPQTMEFAFPLDPCGGAERKAP"
     gene            complement(3258..3416)
                     /locus_tag="AC16BC_02600"
     CDS             complement(3258..3416)
                     /locus_tag="AC16BC_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240535.1"
                     /translation="MTCPKCNGMMYSERLSDYFLTFYAWKCVNCGSIVDHTILENKSK
                     DAPVAASR"
     gene            3601..>3874
                     /locus_tag="AC16BC_02605"
     CDS             3601..>3874
                     /locus_tag="AC16BC_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HIR3240536.1"
                     /translation="MSLAGKSNQTAGGVPTRSYPISRWPSEERPRERLLERGADGLSD
                     AQLIAILLRTGRKQQSAVELAARILSEFGDLRAVAQASTTELCRIDG"
BASE COUNT          703 a         1149 c         1323 g          699 t
ORIGIN      
        1 cgaccacgcc gtggttcaaa tcgttgccgg gctggtggca gcgctatctc ccgcacaagg
       61 gcattacgtt gggattggat ctgcagggcg gcatgcacct ggtgatggag gtcgacgttg
      121 agaaggcggt cgacaacagc ctggaccgca tggcgctcga cctgggggat cgactcaagg
      181 atcagcagat cgcggcgacc gccgcgcggc agggccggtc ggtcgtggtg accatgaccg
      241 acgaggcgga ccgggaaaaa atttccaaaa tcgtggacga acggttcggc gccttgaccc
      301 tcggtgacct caaagacaag gtcctcacct tgaatctgcg cagcggcgag gcggaacgcg
      361 tgcaaaaagc cgcggtgtcg cagtcgatcg aaaccatccg caaccggatc gaccagttcg
      421 gcgtgaccga acccttgatc cagctccaag gcgagcggca gatcctgatt cagttgccgg
      481 gcgaaaaaga tccccagcgc gcgctgaacg tgatcgggaa gaccgcgctc ctggaattca
      541 aactgctgga cgaggacagc ccggtgctcc agcaattgcc cgagttggtg ccggccggcc
      601 aggaacagtc gtggctcgcg gagtggcggg cgaaaattcc tccgggcgac gagatcctgt
      661 tcgaacgggt ggcggaggag aagggcccgg tgaacaagcg gccctacctg gtgaaacaac
      721 gggcgatgat cacgggggag tccctgcggg acgcgttggt cacgatcggc gatttcaacc
      781 aggcgcaggt gtcgattacg ttcgacgccg cggggtccgc ccaattcgag cgcgtgaccg
      841 gggaaaacgt gaagaaacgg ctggccatcg tcctggataa ctcggtctac tccgcgccgg
      901 tcatccagga gcgcatcgcg ggcgggcggg cccagatctc ggggtcgttc acgatggacg
      961 aggccaatga cctggccgtg gtcctgcgcg ccggcgcgct gcccgctccg gtgaacgtga
     1021 tccagaacgt gacggtgggg ccgtcgctgg gccaagactc gatcgacgcc gggattcacg
     1081 cggcctggat cgggaccctg ctggtggtcg cgttcatggc gatctactac cggctggcgg
     1141 gcgtcatcgc cgatttcgcg ctggcgctca acgtcatcct gttgatcggc gcgctggcgg
     1201 cgctcaatgc cacgctgacg ttgccgggca tcgcgggcat catcctcacc atcgggatgt
     1261 ccgtggactc caacgtgctg atcttcgagc gcatacgcga agaactgcgg cagggcaagc
     1321 ccgtccggct ggccgtggac gccggctacg agaaggcctt cctgaccatc gtcgacgcgc
     1381 acgtaaccac gctgatcacc gcggtggtgc tctttctgtt cggcaccggc ccgatcaagg
     1441 gctttgcggt gacgctgacc ctgggcgtca ccatcaacct gttcaccgcg ctggtcggca
     1501 cgaaggtggt cttcgacctc atgaacacac gggcgaaggt cgcccgactg agcatctgag
     1561 gagccccatg ttcgaactac tcggcaagac caacatcgat ttcatcggga aacgccatta
     1621 cgcgtttctc ctctccggcg tgctcgcgtt gctcggggtg atcacggtga tccagatcgc
     1681 ccgcgggcag gccaacacgg ggatcgactt tgccggcggc accgcggtgc aactccagtt
     1741 cgagaaaccg gttcagctgg atgaagcgcg ggcgttgctg gagcgcgagg gcatcccgga
     1801 cgccgagctc cagcagttca cgggcggcaa caaacttctc atccgggtga aaaaacagac
     1861 cacgatccag gagaagaccg cggatcggtt ggtggcggtg ttcaagaaag ggttcgagtc
     1921 caatccattc gtggtggaca gcacgaccga gatcgggccg acggtcggcg caaagctcca
     1981 gcaggacgcg gtgaaggcga tcacgatcgc catgatcggg atcattatct acatcgcgct
     2041 ccggtttgaa tttcgcttcg gcgtggccgc ggcgctcgcc acgttccacg acgtgctcgc
     2101 ggtgctgggg atcatggggt tgctgggcaa ggagatcaat ctgctgatcg tgaccgcgtt
     2161 gctcaccttg gccgggtact cgctgacgga tacggtggtc gtcttcgatc ggatccgcga
     2221 aattctccgg acccgacgac gcgacccgct cgggcaggtg atcaatcagg gggtcaacca
     2281 ggtgttgtcg cgcacgatcg tggtgtcgtt gacggttttt ctcgtcttgc tcgccttgtt
     2341 ctttttcggg ggcgaggtga tccacgactt ttcgttggcg ctgttgatgg gcgtggtcat
     2401 cggtacgtac tcgtcgatct tcgtcgcgag cccgatcctg atgttgtggc ccggcgccca
     2461 agggaagttg ctcaaacgaa ccgcgtagcg tcggtggggc gaggaggccg gtgtgcgcgg
     2521 gttttgacag cggccgttgg gctccgttat catcccgcgc gtccgtgacc atcatccacc
     2581 gacacatgag gggtgaacgc cccgcgctcg aggacaggac gggacgcccc gcccgatgtc
     2641 gatccggccg atggtgggcg ctcgtgctcg tgctggccgc gttgggggcc tgcgcgaccc
     2701 gtccgccggt gttcgaaccg gtgcccgtcg gctcgcggtc cggagcccac gacgtcgtgg
     2761 tccaaatcca accgatcgac acgcagttgg gcagcgagga ccggcggcga tacggcgtgg
     2821 acttcagcgc ctatttcgcc gcgttccgcg tgagcgtgga caatcgcacc gcgcacgcgg
     2881 tggccgcgga tctcgcgccg tcaacactgg gcccgtcggc ggggccgatc accggggtct
     2941 tgagcgacga ggaattggtc cgcatctatc gacgcggggg gatggacagc gacgcggtgg
     3001 aactcgtcgc caaggcgccg tcggtggtca aacgggagct ggagcagatt cgggcggcgc
     3061 gcattcaggc ggctcgcctc gaaccggggg ggcgcgtgga aggggtgttg ttcttccgtc
     3121 cccccgccgg cggagagtgc gtgccctcgg tgttgaccat caagggcatc gcgatccttg
     3181 atgagccgca gacgatggag tttgcgttcc cgctcgatcc atgcggcggg gccgaacgga
     3241 aggcgcccta atcggaatta ccggctggct gcaaccggcg cgtctttact cttattctcg
     3301 agaatcgtgt gatcgacgat cgacccgcaa ttgacgcact tccaggcata gaaggtgagg
     3361 aagtaatcgg acaaccgctc gctgtacatc atcccgttgc attttggaca ggtcatagat
     3421 ctctcctcct tgaggggtaa tggcccggat gactggggag tgaacgcacg ggggacgtaa
     3481 gtgagcaagt gatatgccaa cggacgatct tgcggggatg ggcaaaactg tgctcagggg
     3541 aatgaaaccg gccgtgattc aggcccgtta tgtcaattgc ttgacgcgcg gtgtcaaaaa
     3601 atgtcgctag ccggtaaatc gaaccagaca gcgggcggcg tcccaacccg gtcgtacccg
     3661 atttctcggt ggccgagcga ggagcgtccg cgggaacgac tcctggagcg gggagcggac
     3721 ggactcagcg acgcgcaact catcgcgatc ctgctccgaa ccggtcgcaa acagcaaagc
     3781 gcggtggagt tggcggcgcg gatcctctcg gagttcggcg atctgcgcgc ggtggcccaa
     3841 gccagcacga cggagttgtg tcggatcgac gggg
//
LOCUS       DBNGTT010000112         4551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2067949, whole genome shotgun sequence.
ACCESSION   DBNGTT010000112 DBNGTT010000000
VERSION     DBNGTT010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2067949"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="AC16BC_02610"
     CDS             complement(<1..530)
                     /locus_tag="AC16BC_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3240537.1"
                     /translation="MHATTTKRDFSAQRLALGASLLAGGLVMALSGCTQAAGSPPAPP
                     VPEVVVATVQPRDLPEVFEYVGQVAGVREVEVRARVSGILERWNYTEGAAVRAGESLF
                     TIDQAPFQAELARAEADSARAQANLAQATRTAGRFKPLWEARAVSQNEYDDALSAEQV
                     AGANVKAAEAAVTRARL"
     gene            complement(563..856)
                     /locus_tag="AC16BC_02615"
     CDS             complement(563..856)
                     /locus_tag="AC16BC_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3240538.1"
                     /translation="MLKITRRDEAGTAVIQVEGRLVGPWVKELEECWRSVAAKRALPV
                     RVDLTAVSYIDEAGADLLRTMCRERVDLRVKGCFTTCLIQTIRQAFRRSERNR"
     gene            complement(850..1050)
                     /locus_tag="AC16BC_02620"
     CDS             complement(850..1050)
                     /locus_tag="AC16BC_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240539.1"
                     /translation="MNMTFRMADGTRAGIVPSRELREPAAKQLGVEWTRPDRTVPVVF
                     HVRNQEALDRVLTRFGAAQHAC"
     gene            complement(1597..4194)
                     /locus_tag="AC16BC_02625"
     CDS             complement(1597..4194)
                     /locus_tag="AC16BC_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3240540.1"
                     /translation="MAPHAPQADFRQYRTLLDVSESIAAHRDLKALLRELAQRLLSVA
                     QLEFIGLILHDAERGTMRAYYLGTGEAESVTGLEIPLAESASGWVYNTQQVLVVRNLH
                     EEPRFPAVKAVLDQMGVKSYCLFPLTTAVRRLGAIGFGRKSLSAFPEAELEFLQQVAK
                     QVAIAVDNVLNFESAQVAQQQLAVERDRLRLLLEVSESIASHRDLDELFQDLAQRIPQ
                     VVPFDYINVVLHDARRNVMRLCFLVTAEPSTFSTGLEIPMDESPGALAWKTQQPVTVN
                     DLAQETRFPMLTVKLRENGIQSFCVVPLTTAHRRLGAMGFGSRQPRTYQPAEIAFMRE
                     VAKQVAVAVDNALNAEAAQASQQQLARERDRQQLLLEINNAVVSNLALDDVFTAVSAC
                     LRKVIRHDGSTLVLYNPDTQVCRVHMLDFVNNHNVVEEKQAGPACSSPATIAIKSGKP
                     TLFTERDLTEMSSGSEMAQCILAAGAKSLCCVPLISHQRVLGTLNLARRSEGAFTQED
                     IELLSQVGQQIAIAVENGLVYREIAELKEQLNKEKLYLEEEIRTQYNFEEIIGESAAL
                     KRILKQVETVAPTDSTVLIQGETGTGKELIARAIHNLSGRRERTFVKMNCAAIPTGLL
                     ESELFGHEKGAFTGAIAQKIGRFELAHQGTIFLDEVGDIPLELQSKLLRVLQEQEFER
                     LGSTKTVKVNIRLVAATNRDLTQMVADKEFRSDLFYRLNVFPITVPSLRERREDIPLL
                     VRYFAQKYSQRMDKRVETIPADALAALSKYHWPGNIRELENLIERSVILSHGPDLRVP
                     LGELKVRNAAPADGDATLDGAERRHILRVLKETDWTVGGASGAAVRLGMKRTTLLSRM
                     RKLGITRPS"
BASE COUNT          790 a         1472 c         1408 g          881 t
ORIGIN      
        1 agccttgccc gagtgaccgc ggcctctgcg gccttcacgt tggcgccggc gacttgttcc
       61 gcggacaagg cgtcgtcgta ctcgttctgg ctcacggccc gggcctccca caacggcttg
      121 aaccggcccg cagtgcgcgt ggcctgcgcg aggttcgcct gggcgcgcgc cgaatccgcc
      181 tcggcgcggg caagttcggc ctggaacggc gcctgatcaa tggtaaagag actctcaccg
      241 gctctcaccg cagcgccttc cgtatagttc caacgctcca agatcccgga gacccgcgcg
      301 cggacctcca cctctcggac cccagcgact tgtcccacgt attcgaacac ctcgggcagg
      361 tcgcgcggtt gcaccgtcgc caccaccact tccggaaccg gaggagccgg aggcgaaccc
      421 gcggcctgtg tgcaccccga caacgccatc accaagccgc ctgccagaag gcttgcaccg
      481 agcgcgagtc gttgggcgct gaaatctctt ttggtcgtcg ttgcgtgcat cgcgagtcct
      541 ttcgtcgaaa aactgcgtga agtcatcggt tccgctccga gcgtcggaat gcttgtctga
      601 tggtttggat gaggcaggtg gtgaaacagc ccttgaccct caggtcgacg cgctcccggc
      661 acatggtccg caacagatcc gccccggctt cgtcgatgta actcaccgcg gtcagatcca
      721 cccgcacggg aagagcccgt ttcgccgcga ccgaacgcca gcactcttcg agttccttga
      781 cccagggccc gacgagcctt ccttcgactt ggatcaccgc ggtcccggcc tcatctcgac
      841 gcgtaatttt cagcatgcgt gctgcgccgc tccgaaccgc gtgagcacgc ggtccaacgc
      901 ctcttggttg cggacgtgga acaccacggg aaccgttctg tcgggtcgcg tccactcgac
      961 ccccaactgt tttgcggccg gctcccgtaa ctcccggctc ggcacaatcc ccgccctggt
     1021 tccgtccgcc attctgaatg tcatgttcat cgcctcctcc tttccccgtc acgtggtctc
     1081 gcccgtctct atagcgactc tcgtgccaag acgcgcaacg cggggacaac atcgcggaag
     1141 tgacggaaac tacgagcgag ttgcgagatc gtggtgccgg aggcccgctt gaacagaaag
     1201 accgtgtcga cgcggtgatg tcgaaatgcc gacagatgtc gagagatcga cagtgccaca
     1261 cgtcctcgcg catggggcca tcctgatttt ggggaatctg gtgtttcacg ccctatagtc
     1321 gttcggcaga cgccgaaatc gacatcgagg ttttaggagt ggggtcggcg cggagcgtgg
     1381 ccgactcagc cgtggcgagt gtcgacggat tgtcagttcg aggcgaagcc tcgcaagctg
     1441 ctcaaaaagc tccagccgca aggccgcagg gaggaggcaa cgcggagcgt actgggtcgt
     1501 acgtgaggcg agtggcgcca cgagcgccgc cgagacggtg aggcggccga gactcctcag
     1561 ccctcaacgc cgcggatgga ctttttcagc agcttgctac gaggggcggg tgatgccgag
     1621 cttgcgcatc cgcgacaaca acgtcgtgcg tttcatgccc agtcggaccg cggccccgga
     1681 ggcgcccccg acggtccagt cggtttcttt caagacgcgg aggatgtgac gacgctcggc
     1741 cccgtccagc gtggcatccc catccgcggg cgccgcgttc cggactttca attcgccgag
     1801 cggcacgcgg aggtccggac cgtgggagag aatcacggag cgttcgatca ggttttcgag
     1861 ttcccggatg ttgcccggcc agtggtactt ggagagcgcg gccaatgcgt cggccgggat
     1921 ggtctcgacc cgcttgtcca tgcgctgcga gtatttctgc gcgaagtagc ggacgagcaa
     1981 ggggatgtct tcgcggcgct cacgcagcga cggcacggtg atggggaaca cgttgaggcg
     2041 gtagaaaagg tcgctgcgga attccttatc cgcgaccatc tgcgtgaggt cccggttggt
     2101 ggccgccacc aggcggatgt tgaccttgac ggtcttggtg ctccccagcc gctcgaactc
     2161 ctgttcctgc aacacgcgaa gcaactttga ttgcagctcc agcgggatgt ccccgacttc
     2221 gtcgaggaag atggtgccct gatgcgccag ctcgaatcgg ccgattttct gggcgatggc
     2281 accggtgaac gcgcccttct catggccgaa caactcgctt tcgagcaggc cggtggggat
     2341 cgcggcgcag ttcattttca cgaaggtccg ttcgcggcgc ccgctcaaat tgtggatcgc
     2401 gcgtgcaatg agctccttgc cggtgccggt ttccccctgg atcaacaccg tggaatccgt
     2461 gggcgccacg gtctcgacct gcttcaatat acgcttgagc gccgcgctct cgccgatgat
     2521 ttcttcgaag ttatattgag tgcggatctc ttcttccagg tagagctttt ccttgttgag
     2581 ctgttccttc agctccgcga tctcccggta cacgagtccg ttctcgaccg caatcgcgat
     2641 ctgttgcccc acctggctca acagctcgat gtcctcctgg gtgaatgccc cttcgctgcg
     2701 tcgggcgagg ttgagcgtgc ccagcacgcg ctggtgcgat atcagcggca cgcagcagag
     2761 ggacttcgcg cccgcggcca ggatgcactg cgccatttcc gatccggacg acatctccgt
     2821 caggtcccgc tcggtgaaca gcgtcggttt gcccgacttg atcgcgatgg tcgccggaga
     2881 actgcaggcc ggacccgctt gtttctcttc caccacgttg tgattattca cgaagtccag
     2941 catgtggacg cggcacacct gggtgtccgg gttgtacagc accaaggtcg atccatcgtg
     3001 ccggatgact ttccgcaagc aggcgctgac cgcggtgaac acgtcatcca acgcgaggtt
     3061 ggagaccacg gcgttgttga tctccaggag caactgctgg cgatcccgct cgcgcgccag
     3121 ttgctgctgg gacgcctgcg cggcctccgc gttgagcgcg ttgtccaccg cgaccgccac
     3181 ctgcttggcg acctcccgca tgaacgcgat ctccgcgggt tggtacgttc gcggttgcct
     3241 gctgccgaat cccatggccc ccagtcgacg gtgcgccgtg gtcagcggca ccacacagaa
     3301 cgactggatg ccgttctcgc gaagcttcac ggtcaacatc gggaaccggg tttcctgcgc
     3361 gaggtcgttg acggtcaccg gttgctgggt tttccaggcc agcgcgccgg gcgactcgtc
     3421 catgggaatt tccaaacccg tgctgaacgt gctgggttcc gcggtcacca ggaaacacag
     3481 gcgcatgacg ttgcggcggg catcgtgcag caccacgttg atgtaatcga acggaacgac
     3541 ctgcggaatc cgttgagcga ggtcctgaaa cagctcgtcg aggtcgcggt gcgacgcgat
     3601 ggattccgag acttccagca ggagccgtag ccggtcccgc tcgaccgcca attgttgctg
     3661 ggcgacctgg gcgctctcga aattgagcac attgtccacc gcgatcgcca cttgtttggc
     3721 gacctgttgt aggaactcca gttccgcctc cggaaacgcg ctcaagctct tcctgccgaa
     3781 cccgatcgcg cccaggcgtc gcaccgcggt ggtcagcggg aacaggcagt aggacttcac
     3841 gcccatttga tcgaggaccg ccttgaccgc cgggaagcgc ggctcctcgt gcaaattccg
     3901 cacgaccaat acctgctgcg tgttgtacac ccatccgctg gcggactccg ccagcggaat
     3961 ctccagcccg gtgacgctct cggcttcccc ggtcccgagg tagtacgccc gcatcgtgcc
     4021 ccgctcggcg tcgtgcagaa taagtccgat gaattcgagt tgcgccaccg acagcagccg
     4081 ctgggccagt tcgcgcaaca gcgccttgag gtcgcggtgc gccgcaatgg attccgaaac
     4141 atccaggagg gtccggtact gccgaaaatc ggcctgcgga gcgtgaggag ccacgacgag
     4201 tcctcggatt cgagggaacg ggtctaggta tcggttctaa catcgaccgg cgaagttttg
     4261 caagattgaa cggaaaattg aatggaagac cgtatcaaaa aagggggctt ggcctacctc
     4321 gacgtcgtgg cctctggggg cctcagggac gttacggctt cagtggcatt cgcctcgtcc
     4381 cttccgcccc catacagtcc acttcggtca aatggtgaag aacaccccgg gcgggctgac
     4441 gaacgctccc gatcgcccga accaccggag cagcgcgcgc ttgagagatt ctcgctccaa
     4501 cggtttcggg aggacctcgt taaactcggg gccggcgaac ttccagggct c
//
LOCUS       DBNGTT010000113         5452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3344717, whole genome shotgun sequence.
ACCESSION   DBNGTT010000113 DBNGTT010000000
VERSION     DBNGTT010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3344717"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /gene="holB"
                     /locus_tag="AC16BC_02630"
     CDS             complement(<1..672)
                     /gene="holB"
                     /locus_tag="AC16BC_02630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR3240541.1"
                     /translation="MSAPTRFHDVVGHGPIIARLRAALAGQRLGSAYLFVGEPEIGKG
                     ALALIWARLIQCEAPIGSAQELEPCGACRSCRQHDAAGHPDLFVLEPDDASVITIDQV
                     RRLQAELPYRPLTSTRRVVIIPEAARLNAESANGLLKILEEPPGHAMFLLVTSQRDRL
                     LPTILSRCQMIRCAPPPPEAILGHLTGRLGLAPAEARRVLALSQGRIGPAIVAAANPG
                     ADAAGF"
     gene            complement(669..1343)
                     /gene="tmk"
                     /locus_tag="AC16BC_02635"
     CDS             complement(669..1343)
                     /gene="tmk"
                     /locus_tag="AC16BC_02635"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3240542.1"
                     /translation="MSGRPTSPGRFPGRFITFEGVDGSGKSTQLAALASALTARGIPV
                     VTTREPGGTPLGEAIRRVVLDQSFAGMSARAELLLYLASRAEHVDRVIRPALERGALV
                     LCDRFTEATLAYQAYGRGLPIDDVTALTRFAAADIEPDLVVLLDLPVEDGLARVRERR
                     GLNRLDRETVAFHGRVRDGYLALAESAPDRFKVFDARRPVNELHAGIAAAVEPLCGVT
                     ASGGRG"
     gene            complement(1340..3175)
                     /locus_tag="AC16BC_02640"
     CDS             complement(1340..3175)
                     /locus_tag="AC16BC_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3240543.1"
                     /translation="MIIIELACLGIRSFRQVTKLTLKPGLSIIQGRTGSGKTTLVECL
                     KVLLFGAPAETLGPLSIPAGGTAQAAVTVKLRNGDIYRVIRDFGKDMLQILKFDMGTR
                     AFAPVASDPSALAALWSAECDGLTLADAEAVSVWSPRTSLPTGAAAETPPWGSDSLSA
                     GPAPAASIVLTPEQRAAKVEQLAQLQGRLARAEQQAESADERANAVAQEADVRRKLAA
                     LDALAARREGAAARKDEMAPFLQGPENLDALLDGYIKAVPAVETERTQLTEESAALAI
                     RIEEAASRPFLKAPMFWAGAGVTALSFIVALTVALSGWFRAVYLVGLVAGLGLLVTSL
                     VMDFRRLAAKRAAEDKLADAQKKLARLDDRLKKTYAAPVALIAQTNSGDAETFKAKRR
                     AAKEWAAELQAFDEQEKAMLAGKTRSDLDAHWQAAKTRADALVRQGGDEVDVESLRDA
                     IRKLTQELESAPAPSSARSAAVQPAAAGAGFGFAPVRDHAADIGRCLAKLSNARLDAV
                     CELNGSVGVRRRGTTDAAPIESLSSGEAIETRLAITLGSWAARRAGLGLPLILDDPLS
                     GLDQEARHALIATLAAVVGDRQILLLTNAPVPAAAGLTQIPLPAA"
     gene            3261..3995
                     /locus_tag="AC16BC_02645"
     CDS             3261..3995
                     /locus_tag="AC16BC_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR3240544.1"
                     /translation="MHHVEKLSRVVARGASRVPGVDRGQGGRARGVGGGIGIRSRLKR
                     YFVTGLLVVTPLWGTYLILKGLFFSLEGVLGNLLASYTQFYIPGLGIAVLVVLLLFVG
                     MMATNIFGRKLVQSWEELLKHVPVVKTIYQVLKAVVDTFSIHNREQFSRVVLVEFPRK
                     GQYSIAFVTGITRGEIQRITPDRLVNVYVPTTPNPTSGYFLFVPEQEIIPLSMSVEDG
                     MKMIISGGMYTPASGEEPVASGSGAQ"
     gene            3992..5440
                     /gene="glmU"
                     /locus_tag="AC16BC_02650"
     CDS             3992..5440
                     /gene="glmU"
                     /locus_tag="AC16BC_02650"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR3240545.1"
                     /translation="MSGAKAGVAVVVMAAGQGKRMKSALPKVLHPVGGRPMVEYALRA
                     ADALAPAHVVVVVGHGGDAVAEWVGTRALIARQDPPRGTGDAVRCALSRIPGFSGTVA
                     VLSGDTPLVRAETVQRMVARHRESGAAVTLATMRIEHPTGYGRVLAGPDGRVTGVVEE
                     RDATTDQRAIRDVNAGLYALDAAFLRGALESLKPDNAQGEYYLPDVIGLAVEAGLAVR
                     GEAVDPEEVLGVNTRAELARVEAVMRERIRRRWMAEGVTLLDPARIWSDDAVAIGPDT
                     VIAPGAWLEGETVVGSGCRIGPSSHLTDSRLGDRVTVKDGCVIDDAVIEDGASVGPFA
                     HLRPGTILRRDARVGNFVELKKADLGAGSKANHLSYLGDAVIGADVNIGAGTITCNYD
                     GVKKSKTVIEDGVFIGSDSQLVAPVTVGKGAIVAAGTTVTQDVPPDALVIGRVRQTVK
                     TGWVKTRMAAARKAAGRGRESSARGRKTKKSP"
BASE COUNT          814 a         1789 c         2030 g          819 t
ORIGIN      
        1 gaacccggcc gcgtccgcgc cggggttggc ggccgcgacg atggcgggtc cgatccgtcc
       61 ctgcgacagg gccaggacgc gccgcgcctc cgcgggcgcg agcccgagcc gtccggtgag
      121 gtggcccagg atcgcctcgg gcggcggggg cgcgcaccgg atcatctggc agcgcgacag
      181 gatcgtgggc agcaggcggt cgcgttgcga ggtgacgagc agaaacatcg cgtggcccgg
      241 cggttcctcc agaatcttga gcagcccgtt cgccgattcc gcgttcaatc gcgcggcctc
      301 cgggatgatc acgacgcggc gcgttgacgt gagcgggcga taggggagtt cggcctgcag
      361 ccggcggacc tgatcgatgg tgatgaccga cgcgtcgtcg ggctccagca cgaagaggtc
      421 ggggtgcccg gccgcgtcgt gctgccggca cgagcggcac gcgccgcacg gttcgagctc
      481 ctgggcggag ccgatcggcg cttcgcactg aatgaggcgc gcccagatca gggccagcgc
      541 gcccttgccg atctcgggtt cgcccacgaa caggtaggcg gagccgagtc gttgtccggc
      601 cagcgcggcg cgcagcctgg cgatgatcgg tccgtgcccg accacgtcgt gaaaacgcgt
      661 gggcgcgctc acccgcgtcc tcccgacgcc gtgaccccgc acaacggttc cacggccgcc
      721 gcgatgccgg cgtggagttc gttcacggga cggcgggcgt cgaagacctt gaaacgatcg
      781 ggcgcggatt ccgcgagcgc gaggtatccg tcgcgcaccc ggccgtgaaa cgcgacggtc
      841 tcgcggtcca ggcggttgag gcctcggcgc tcgcgcacgc gggccagccc gtcctccacc
      901 ggcaggtcca ggagcaccac caggtcgggc tcgatgtcgg ccgccgcgaa ccgggtcaac
      961 gccgtgacgt cgtcgatcgg caggccgcgc ccataggcct ggtaggcgag ggtggcctcc
     1021 gtgaaccggt cgcacaacac cagggccccg cgctccagcg ccgggcggat cacccggtcc
     1081 acgtgttcgg cgcggctggc caggtacagc agcagctccg cgcgggcgga cattccggca
     1141 aaggattggt cgagcaccac gcgtcgaatc gcctcgccca gcggggtccc gcccggttcg
     1201 cgggtggtca ccaccgggat gccgcgcgcg gtcagcgcgg acgcgagcgc cgcgagctgc
     1261 gtggacttgc cgctcccgtc gacgccttca aaggtgatga accggccggg gaaccggccc
     1321 ggcgaggtgg gacggccgct catgccgcgg gcaacggaat ctgcgtgagt ccggccgcgg
     1381 ccggaaccgg cgcgttggtc aggagcagga tctggcggtc gcccacgacc gcggccagcg
     1441 tggcgatcag ggcgtggcgc gcttcctggt ccagaccgga gagcgggtcg tccaggatca
     1501 agggcaggcc gagccccgcg cgccgcgcgg cccatgagcc caacgtgatc gccagccgcg
     1561 tctcgatcgc ttcgcccgag ctgagcgatt cgatcggggc cgcgtcggtc gttccgcgcc
     1621 gtctcacgcc caccgagccg ttcagttcgc agaccgcgtc gagtcgcgcg ttggaaagtt
     1681 tggcgaggca ccggccgatg tcggccgcgt gatcgcgcac cggagcgaat ccgaagcccg
     1741 cgcccgccgc ggccggttgg accgcggcgc tccgggcgga cgagggggcc ggcgcgctct
     1801 ccaactcctg ggtcagcttc cgaatcgcgt cgcgcaggct ttccacgtcg acctcgtcgc
     1861 cgccctggcg tacgagcgcg tcggcgcgcg ttttggcggc ctgccagtgg gcgtcgaggt
     1921 ccgagcgcgt tttgcccgcg agcatcgcct tctcctgctc gtcgaaggct tgcaactccg
     1981 cggcccattc cttggccgcg cggcgtttgg ccttgaacgt ctccgcgtct ccggaattgg
     2041 tctgcgcgat cagcgccacg ggcgccgcgt aggttttttt gaggcggtcg tcgagacgcg
     2101 cgagtttttt ctgcgcgtcg gccagcttgt cttcggccgc gcgtttggcg gccaggcgac
     2161 ggaagtccat caccagcgag gtcaccagca ggccgagccc cgcgactagg ccgaccaggt
     2221 agacggcgcg gaaccagccg gagagcgcca cggtcagggc tacgatgaag gacaaggcgg
     2281 tcacgcccgc gccggcccag aacatcggag ccttcaggaa gggccgggac gcggcctcct
     2341 cgatcctgat cgcgagcgcg gccgattcct cggtcaactg agtgcgctcg gtctccacgg
     2401 cgggcacggc tttgatgtag ccgtcgagca gcgcgtcgag gttctcgggg ccttgaagga
     2461 acggcgccat ctcgtccttg cgcgcggccg cgccttcccg gcgggccgcc agcgcgtcca
     2521 gcgcggccag tttgcggcga acgtccgcct cctgcgccac ggcattggcc cgttcgtccg
     2581 cggattcggc ctgctgttct gcgcgcgcca gacgtccctg cagctgcgcg agttgttcga
     2641 ctttggccgc gcgttgctcg ggggtgagga cgatcgaggc cgctggcgcg ggtccggccg
     2701 acaagctgtc cgagccccac ggcggcgtct cggccgcggc gcccgtcggc agggacgttc
     2761 gcggcgacca gaccgagacg gcctccgcgt cggcgagcgt caacccgtcg cactcggcgc
     2821 tccacaacgc ggccaacgcg gacggatcgg acgccaccgg cgcgaacgcg cgcgtgccca
     2881 tgtcgaattt gagaatttgc agcatgtcct tgccgaaatc gcggatcacc cgataaatgt
     2941 cgccgtttcg cagtttgacc gtcaccgcgg cctgcgccgt gccgcccgcg gggatcgaca
     3001 acggccccag cgtctccgcc ggagccccga acaacaacac cttcaggcac tcgaccagcg
     3061 tggttttgcc cgagccggtt cgtccctgaa tgatgctgag ccccggtttc agggtcaact
     3121 tggtcacctg gcggaaggag cggatcccca ggcaggcgag ctcgatgatg atcatccgat
     3181 cggggcggcg tgcgtcagca cgcgcggaat cctacgtgac ttcgtggaaa attgcaaacc
     3241 gcgcgcaacc gcgatatgtc ttgcatcacg ttgaaaaact tagtagagtg gtcgcgcgcg
     3301 gcgcgagtcg ggtcccgggc gtggaccgcg gccagggggg ccgtgcgcgc ggcgtcggcg
     3361 gagggatcgg catccggagt cgactgaaac ggtatttcgt gacgggcctg ctggtcgtca
     3421 ccccgctgtg ggggacgtac ctgattctga aaggtttatt cttctccctg gaaggcgttc
     3481 tcggcaatct cctcgcgtcg tacacgcaat tttatatccc cgggctgggg atcgcggtgc
     3541 tggtggtgtt gctgctgttc gtgggcatga tggccaccaa catcttcggc cgcaaactcg
     3601 tgcagtcctg ggaggagctg ctcaaacacg tgccggtggt caagacgatc tatcaggtgt
     3661 tgaaagccgt ggtggatacg ttttccatcc ataatcgcga acaattcagc cgggtggtgc
     3721 tggtggaatt cccgcgcaag gggcagtact cgatcgcgtt cgtgaccggg atcaccaggg
     3781 gcgaaatcca gcggatcacg cccgatcgtc tggtcaacgt gtacgtgccc accacgccga
     3841 atccgacgtc gggctatttc ctgttcgtgc cggagcagga gatcattccc ctctccatga
     3901 gcgtggagga cggcatgaag atgatcatct ccggcggcat gtacacgccg gcgagcggcg
     3961 aggagccggt cgcgtccggc tcgggcgcgc aatgagcggg gcgaaggcgg gcgtggccgt
     4021 ggtggtgatg gccgcggggc agggcaaacg catgaagtcg gccctgccca aggtgctgca
     4081 cccggtgggc gggcgcccga tggtggagta cgccctgcgg gccgccgacg cgctcgcgcc
     4141 cgcgcacgtc gtggtggtgg tggggcacgg cggcgacgcg gtcgcggaat gggtcgggac
     4201 gcgcgcgctg atcgcgcggc aggatccgcc gcgaggcacc ggcgacgcgg tgcggtgcgc
     4261 cttgtcgcgg atcccggggt tctcgggcac ggtggcggtg ttgagcgggg acacgccgct
     4321 ggtgcgggcc gagaccgtgc agcgcatggt cgcccgccac cgcgagtccg gcgccgcggt
     4381 gacgctggcg accatgcgga tcgagcatcc gaccgggtac gggcgcgtgt tggcgggccc
     4441 ggacggacgc gtgaccggcg tggtggaaga gcgcgacgcc accaccgacc agcgggccat
     4501 ccgcgacgtc aacgccgggt tgtacgcgct ggacgcggcc ttcctgcgcg gcgcgttgga
     4561 atcgctcaag cccgacaacg cgcagggcga gtattatctg ccggacgtga tcggcctcgc
     4621 ggtcgaagcg ggcctggcgg tgcgcggcga ggcggtggac cccgaggaag tgctgggggt
     4681 gaacacgcgg gcggagttgg cccgggtgga ggccgtgatg cgcgagcgca tccggcggcg
     4741 ctggatggcc gagggcgtga cgttgctcga cccggcccgg atctggagcg acgacgcggt
     4801 cgcgatcggc ccggacaccg tgatcgcgcc cggggcgtgg ctcgaaggcg agaccgtggt
     4861 gggatccgga tgccgcatcg gcccgtcgag ccatctcacc gacagccggc tcggggaccg
     4921 ggtcacggtc aaggacggct gcgtgatcga cgacgcggtg atcgaagacg gcgcgtcggt
     4981 cgggccgttc gcgcacctgc gaccgggcac gatcctgcgg cgcgacgcgc gggtgggcaa
     5041 tttcgtcgaa ttgaagaagg cggacctggg ggcggggtcc aaggcgaacc atttgagtta
     5101 tctgggcgac gccgtgatcg gcgcggacgt gaacatcggc gcggggacga tcacgtgcaa
     5161 ctacgacggg gtgaagaagt cgaagaccgt catcgaggac ggcgtgttca tcggcagcga
     5221 cagccagttg gtggcgccgg tgacggtcgg caagggcgcg atcgtcgcgg ccggcaccac
     5281 ggtgacgcag gacgtgccgc cggacgcgct ggtgatcggc cgcgtgcggc aaacggtaaa
     5341 aacggggtgg gtcaagacgc ggatggccgc ggcgcgcaag gccgcggggc gcgggcggga
     5401 atcctccgcg cgcgggcgaa agaccaagaa atccccgtaa tccagaaacg ga
//
LOCUS       DBNGTT010000114         1749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_662873, whole genome shotgun sequence.
ACCESSION   DBNGTT010000114 DBNGTT010000000
VERSION     DBNGTT010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_662873"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="AC16BC_02655"
     CDS             <1..391
                     /locus_tag="AC16BC_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3240546.1"
                     /translation="AMPKARRIGWMMGTVMWMVSALPAIATELTTIDGPQLQALMADG
                     KQVVIVDVREPELFAKGHIPGAINIPYDDAKPRILRELRPQDRIVFVCHGGPMGDELG
                     RLLAGRGYPSVYNLKGGMKKWKGAEEK"
     gene            complement(497..1474)
                     /locus_tag="AC16BC_02660"
     CDS             complement(497..1474)
                     /locus_tag="AC16BC_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240547.1"
                     /translation="MTVIQRFPWSSWKVTGWSHRPAFTPGRVTIPRGGWCVPSAIFVA
                     SLVAVTPELSVAAPSVFAPAPMVLGHTGVVAINSTDVLAPGAIRAGIGALFATGNGGN
                     RVDLLLDDDASRDRGMTARASLALGLPGAIEAALTVPYSNNETGNVSIDGLGDATVSA
                     KLRLWAQRGWRPATAASVSWIPETAREPEVRSVTTNGYLATMSTQLGLITGDWTWTII
                     GELGGFWRDPGRAESDSSFIYGLAGVVPLTRTGLVEDTGELQLIAEVTGSSARRSLAH
                     EPDDAVSFAPGFRYLSPSWGVTVTGVFTRYERPTKNAGAGGMMTFHAVF"
BASE COUNT          337 a          537 c          583 g          292 t
ORIGIN      
        1 ggctatgccg aaagcgcggc ggatcggatg gatgatgggg acggtgatgt ggatggtctc
       61 cgcgttgccg gcaatcgcga ccgaactcac gaccatcgac gggccgcagc tccaggcgtt
      121 gatggccgac gggaagcagg tcgtgatcgt ggacgtgagg gaaccggagc tatttgcgaa
      181 aggccatatt cccggagcca tcaatatccc ctacgacgac gcgaaaccgc ggatcctgag
      241 agaactccga ccccaggatc gcatcgtctt tgtctgccac ggggggccga tgggcgacga
      301 attggggcgg ttgctcgcgg ggcgagggta tccgtcggtc tataatctca agggcggcat
      361 gaagaagtgg aaaggcgcgg aggaaaagtg acgtcgcagc gggctgaacg gcgatccaag
      421 cacgaggcct tggtgatcga cccaacagaa ggcttgagga cgtcggtccg cgccatgacg
      481 ggcggtgctc gcccgctcag aacaccgcgt ggaatgtcat cataccgccc gcgccggcat
      541 ttttcgtggg gcgttcgtag cgcgtaaaga ctccggtgac cgtcacgccc catgatgggg
      601 aaaggtaccg aaatcctgga gcgaaggaaa ccgcgtcatc cggttcatgc gcgaggctgc
      661 gtcgggccga cgagccggtg acctcggcga tcaattggag ttcccccgta tcttcaacga
      721 ggccggtccg ggtcaacgga acgacgccgg ccaaaccgta gatgaacgat gagtcggatt
      781 cggctcgccc cgggtctcgc caaaatccgc ccaactcgcc gatgatcgtc cacgtccaat
      841 cccccgtgat caggccgagc tgggtcgaca tcgtggcgag gtatccgttg gtggtgacgc
      901 tgcggacttc cggctcgcgt gccgtttccg ggatccaaga aaccgacgcg gcggttgcgg
      961 gacgccaacc gcgttgagcc cataggcgca gcttggccga cacggtcgcg tctccgagtc
     1021 cgtcaatgga cacgttgccg gtctcattgt tgctgtaggg gacggtcaac gcggcctcga
     1081 tggctcccgg cagccccaag gccagcgacg cccgcgcggt catcccccga tcgcggcttg
     1141 cgtcgtcatc caacagcagg tcgacgcggt ttcccccgtt tccggtggcg aagagggctc
     1201 cgattccggc gcggatcgct ccgggggcca atacatcggt cgaattgatg gccacgacgc
     1261 cggtatgtcc cagcaccatg ggggctggcg cgaacactga cggcgcggcg acgctgagtt
     1321 caggagtcac ggcgactaac gacgccacga aaatggccga cgggacgcac catcccccgc
     1381 gagggatcgt cacacgcccg ggagtgaacg cgggacgatg cgaccatccg gtcaccttcc
     1441 agcttgacca cgggaaccgt tggatgacgg tcatgagaag gacctcgaca aatctgcgcg
     1501 atcgtagcga tcgaagcatg ggggtgtcaa ggggagccac tcaggctgcg tgtgcgtgtc
     1561 acgtggtctg cccgcgtgac gagcaaggcc tcctccgggc acatgccggc cgcgagtgat
     1621 ttgacgaacc gcgcgcccct gactaccctt cccccgtcac cgggcgggag acttcaccgt
     1681 gatccagaac cgcatcgttc tccactatag cgacgggcgt atcgttaagg gcagcaccgg
     1741 ggatttctt
//
LOCUS       DBNGTT010000115         3010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1045467, whole genome shotgun sequence.
ACCESSION   DBNGTT010000115 DBNGTT010000000
VERSION     DBNGTT010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1045467"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /locus_tag="AC16BC_02665"
     CDS             complement(<1..1978)
                     /locus_tag="AC16BC_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3240548.1"
                     /translation="MVGADPGSPVRRSVQGLFGRLWSVVPQGGSLPDEIWQERHRFMV
                     GFTWFHALLIAVLGPTLGARGHDGVILQSVIGAGVVAGFAALAGVRALGRIGRAAAVG
                     VGLMTSSALLVHLSGGYIELHFHFFVMIIFMALYQDWIPYLLAILYVAIHHGVVGVLW
                     PTMVYNHPAAINAPWTWAGIHAFFVLWASVGSIVAWRYTEHAFGRARLLLESTGEGVF
                     GLNRDGHVMFINSAATRMLGLRGVRGMGQPITRLVRHTKANGAPYPDRDNPMLNPLNT
                     GIACVVVDELFWREDGTSFAVDFRSSPIMERGAVTGVVVTFSDVTERKKATAAIQASE
                     ARYAGILDIAHEAIISIDEAQRIVVFNQGAEKIFGYSQAEILGAPLDRLLPARFHDAH
                     RAHVDGFAGRGEGSRRMQERREVSGLRKDGTEFPAEASISNLELNGERICTVVLWDIT
                     SRHHADAERKTSLSLLSATVESTADGILVVNQAGKIIHYNSKFMAMWRIPEAILAAGD
                     DQRAIECVLDQLEDPPAFVEKVMALYATPEAESHDRLAFKDGRVFERYSQPQRIDGRS
                     VGRVWSFRDITERVRAEEEVRLLHTMTRLISEADDLHTALQRVVQTVCEATGWLGGQA
                     WTPRADGRLECSPAWYDAAASLQIIRSASETVAFG"
     gene            complement(2343..2693)
                     /locus_tag="AC16BC_02670"
     CDS             complement(2343..2693)
                     /locus_tag="AC16BC_02670"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3240549.1"
                     /translation="MPEPERRYFRRVQKVIEFHCTSHGPPLTGRIRDLSEGGVFVDTA
                     NPLPAGTPVTFTFVLQPISKKTILGRGLVRWCEDEVGMGIQFLALSEDDQELIRRFVA
                     LDDRTSPGPGSPPP"
BASE COUNT          554 a          981 c          963 g          512 t
ORIGIN      
        1 ccccgaacgc gacggtttcg ctcgcggacc ggatgatctg caggctggcg gcggcgtcgt
       61 accacgcggg gctgcactcg agtcgaccat cagcccgggg ggtccaggct tgtcctccca
      121 accacccggt cgcctcgcac acggtctgca ccacccgctg caacgcggtg tgcaggtcat
      181 ccgcctcgct gatcaggcgc gtcatcgtgt gcaggaggcg gacttcttcc tcggcgcgga
      241 cccgctcggt gatgtcccga aaactccaga cgcgtcccac gctgcggccg tcgattcgct
      301 ggggctgcga gtaccgttcg aacactcggc cgtctttgaa cgccaagcga tcgtgactct
      361 ccgcctcggg cgtggcgtac aacgccatga ccttttcgac gaacgcgggg gggtcctcca
      421 gttggtccaa cacgcactcg atcgcccgct gatcgtcccc cgcggccagg atggcttcgg
      481 ggatgcgcca catggccatg aacttgctgt tgtagtggat gatcttcccg gcctggttga
      541 ccaccaggat gccgtcggcg gtggactcca cggtggccga caacagcgag aggctcgttt
      601 tgcgctcggc gtccgcgtgg tgccgactcg tgatgtccca gagcaccacg gtgcagatcc
      661 gctctccgtt caattcgaga ttggagatgg aggcctcggc cggaaattcc gtgccgtcct
      721 tgcgcaggcc cgagacttcc cgccgttcct gcatgcggcg gctgccctcg ccgcggccgg
      781 cgaacccgtc cacgtgcgcg cggtgcgcgt cgtggaatcg cgccgggagc agccgatcga
      841 gcggcgcgcc cagaatctcg gcctgggaat agccgaaaat tttctcggcg ccctgattga
      901 acaccacgat gcgctgggcc tcgtcgatcg agatgatggc ctcgtgggcg atgtccaaga
      961 tccccgcgta tcgggcctcg ctcgcctgga tcgccgcggt ggctttcttg cgttcggtca
     1021 cgtcgctgaa cgtcaccacc accccggtca cggcccctcg ttccatgatc ggggagctgc
     1081 gaaaatccac ggcgaacgag gtgccgtctt cgcgccagaa caactcgtcc accacgacgc
     1141 acgcgatccc cgtgttcaac gggttcaaca tcggattgtc gcggtccgga tacggcgcgc
     1201 cgttcgcctt ggtatggcgg accaatcggg tgatcggctg tcccatcccg cgaacgcccc
     1261 tgagcccgag catccgggtc gccgcggaat tgatgaacat cacgtggccg tcccggttga
     1321 gcccgaacac gccttctccg gtggactcga ggagcaatcg cgcccgtccg aacgcgtgtt
     1381 ccgtgtaccg ccaggcgacg atgctgccga ccgaggccca caacacgaaa aacgcgtgga
     1441 tgccggccca ggtccacggc gcgttgatcg cggccggatg attgtacacc atggtcggcc
     1501 acagcacgcc caccacgccg tgatggatcg ccacgtacag gatggccagg aggtagggaa
     1561 tccaatcctg gtacagcgcc atgaagatga tcatgacgaa gaaatgaaag tggagttcga
     1621 tatacccgcc cgacaggtgg acgagcaggg cggacgacgt catgagcccg acgcccaccg
     1681 ccgcggcccg accgatgcgg ccgagcgcgc ggaccccggc caacgccgca aacccggcga
     1741 ccacgcctgc gccgatgacc gactgcagga tcacgccgtc gtggcctcgg gcgcccagcg
     1801 tcggaccgag gaccgcgatc aacagggcat ggaaccaggt gaagccgacc atgaagcggt
     1861 gccgctcttg ccagatttcg tccggcaaac tgccgccttg cgggacgacg gaccacaagc
     1921 gaccgaacag tccctgcacg gaacggcgca ccggcgatcc ggggtcagcg ccgaccacga
     1981 cgcgcccttt caccgatggt ggtgtgaccg atcaagacgt cttgcccatc aagcggcacc
     2041 cctgaacaca gcaccgtgga gtcgtccaag aaaacgtccg agcgaccgtg aatcgcgcca
     2101 ccgcgggcgc aggctgattc cgcgtccgcg gtggatgttt cgtcaatttt ctctaatgat
     2161 agtggagcgg cctcgggttg aacgtagcac cgatggtggc gatgtcaatc ggagccgcgc
     2221 tgacggatcg ctccgatgtc gccttgctcg tggttgagcg cgggcaatgc gggagtatcg
     2281 ggatcaggaa caaacgaacc tgcagcgttc ggtccgtgtc gccgttctgt cttgggttcg
     2341 cgctagggag ggggcgagcc ggggccgggg gatgtccggt cgtccagggc aacgaaccgc
     2401 cggatcaact cttggtcgtc ttccgacaaa gcgagaaact ggatgcccat gcccacttcg
     2461 tcttcgcacc agcggaccaa gccccgcccc agaatggttt tcttcgagat cggttgcagg
     2521 acgaaggtga acgtcacggg agttccggcc ggcagcgggt tcgccgtatc gacaaacacg
     2581 ccgccctcgc tcaggtcccg tatccggccg gtgagcggcg gcccatggct cgtgcagtgg
     2641 aactcgatga ccttctgaac acgccggaag taccgtcgct cgggctcggg catcgcgcac
     2701 cctccgcgct tgggtgagaa ggcctgattc gggatggcat ggtagcacac tggtcgcaaa
     2761 agatcctgat cagggctatg ccttgggtgg tgtcagggga ctcgaccagg agcgcaaaac
     2821 ggctcgcgac ggacagtgtc gattcaggag cctttgtccg gcgcgcaacg aagaaaacaa
     2881 accagtgagg tcaagcgaag acgggaatag gcggcttcga tcatgtgagg tctgaacagg
     2941 acaggcggat tcgattgtca ccaagccgca attggtttaa accattcagc atgcgcaggc
     3001 tcacacaccc
//
LOCUS       DBNGTT010000116         3451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3218346, whole genome shotgun sequence.
ACCESSION   DBNGTT010000116 DBNGTT010000000
VERSION     DBNGTT010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3218346"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2577
                     /locus_tag="AC16BC_02675"
     CDS             <1..2577
                     /locus_tag="AC16BC_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3240550.1"
                     /translation="AAIDPPSNTPGGLIYTVQYKRRVDAGFSSMPSGIGGKSVTLTGL
                     VASTEYQIRLVVSDQSANQADVVLEAAPTFTPSAGRLYVANKVYNAVGVFSDVGQASG
                     NKPGGLVTADQTGLVAPTGIAVDRDPADPTQLDPDGYVYVAEAAANRIAAYKLCPTGN
                     CQALQLGQFSIGHNVAPAWTIEGDLASTDLTELCGPATLRLERRADGRKVLYAANSLV
                     LPVGSSSPACVPNKIVAFDVTRPPQGTNQPPMTRIERPRSFFSPLAFTLNEQAKLLFV
                     ANRDDLNGVGNNAGSTIDVYSYAMYMESADQTPHRTFWGSADNPCVAPASEPGDHLCG
                     PTALAYDALYDRLYVINRGKSDLLVFSDVSSPLTVGRQVPAVVQGANTGLDGARPVGI
                     FLDSVTRRVYVTTDTGQSVVIFDADELAVGGNVPPRRVIKGTKTLLGQPTKDSTNQSS
                     GPFAVTVVGTEAFVVTPGIYPSGTGLTPLPSMTVFNVSDGADPTVLATDPLSARTTNT
                     VPSRALVDPMLGASAVALDPQSGRLYVAGFDANMILVFDDPQSFATGQIAPDRILAGP
                     STRLDHPVALLFRRQDASANGSLYVVNQSSHSVSVFLEGDGAAAPLLAGDRAPARYIG
                     PPDGTDPFSVTANVPQMVFPTGLTIDPGVAADLTDDILYVSNRDAESFQDLVGRRIVA
                     FQDANTVNGAVAPTWKVEGDRPPTGSAKATDKTTLKRPAGLWMIPDPLPNDGTPDDRL
                     VVANRDGKSVLIFRGLRALVAQAAANPGAPPEDNRAPAWTIKHFKLVAPFGVVFEQAS
                     RQLYVSDIAGRILAFDLTDLDTDLADLDIDTPEPALTPRVVEGASTGLAAPLGLALDP
                     QN"
     gene            2643..>3451
                     /locus_tag="AC16BC_02680"
     CDS             2643..>3451
                     /locus_tag="AC16BC_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240551.1"
                     /translation="MSVALAAALSGCAGPAPLREVRSPEPPGASRALDQCAERCFAEA
                     KAALAAGDVATAQDRLRGVRRAAPNTRWAGRAALLLARVDAERDPEAGLRYALQAAAD
                     LPELGDHALAVAADAAQHGGRPVQAAGYLHELARRYPQSSLVPTALWSAAALWAPLDG
                     RQGEAIAALTALSTDFPSDPRVPEALAKIVSLAAPSGRREEAALACRRLVVDFPASPD
                     AASVAESCLKALRDDGAPLTFSELRRRAEGLARGAKFSEALDVWQDLKRSAP"
BASE COUNT          535 a         1152 c         1181 g          583 t
ORIGIN      
        1 gcggccatcg acccgccgag caacaccccg ggcggattga tctacaccgt ccagtacaag
       61 cgccgggttg acgcggggtt ttcgtccatg ccgtccggca tcggtggaaa gagcgtcacg
      121 ctcaccggct tggtggcgag caccgaatat cagatccgtt tggtcgtcag cgatcagtcc
      181 gccaaccagg cggacgtggt cctggaagcc gctcccacgt tcacgccgtc cgctggccga
      241 ctgtatgtcg ccaacaaggt gtacaacgcg gtgggggtct tctcggacgt cggccaagca
      301 tcgggcaata agccgggtgg gctggtgacc gcggatcaaa ccggcctggt ggcgccgacc
      361 gggatcgcgg ttgaccgcga tcccgccgac ccgacgcagc ttgatcccga cgggtacgtg
      421 tacgtggcgg aggccgcggc gaaccggatc gcggcgtata agctctgtcc caccgggaat
      481 tgccaggccc tgcaattggg ccagttcagc atcggccaca acgtggcgcc cgcatggacc
      541 atcgagggcg atctggcgag cacggacttg accgagttgt gcggacccgc gacgttgcgt
      601 ctggagcggc gcgccgacgg ccggaaagtg ctctatgccg cgaactctct ggtcttgccc
      661 gtgggcagtt cgtcgccggc ctgcgtgccg aacaagatcg tggcgttcga cgtcacgagg
      721 ccgccgcagg gaaccaatca gccgcccatg acgaggattg agcgtccgcg gtccttcttc
      781 agcccattgg cgtttacgct caacgaacag gccaaactcc tgttcgtggc gaatcgcgac
      841 gacctcaacg gggtgggaaa caacgccggg tccacgatcg acgtgtactc gtacgccatg
      901 tatatggaga gcgcggatca aactccccat cgaacgttct ggggctcggc ggataatccg
      961 tgtgtggctc cggcctccga accgggcgac cacctgtgcg gtcccaccgc gttggcatac
     1021 gacgcattat atgatcggtt gtatgtgatc aaccggggca agagcgacct cttggtcttc
     1081 tcggacgtct cgtctccgct gaccgtcggc cgacaggttc cggcggtggt gcagggggcg
     1141 aatacgggcc ttgacggggc gcggccggtc gggatcttcc tggattccgt cactcgccgc
     1201 gtctacgtga cgacggacac cgggcagtcg gtggtgatct tcgacgcgga cgaactggcg
     1261 gtcggcggaa acgtgccgcc gcggcgcgtg atcaagggca cgaagacgtt gctcgggcaa
     1321 cccaccaaag actcgaccaa ccagagcagc gggccgtttg cggtcaccgt ggtgggcacc
     1381 gaggcgttcg tcgtgacgcc gggcatctac ccgagcggga ccggcctcac gccgctcccc
     1441 tccatgaccg tcttcaacgt gtccgacggc gctgatccga cggtgcttgc caccgatccg
     1501 ctgtcggccc ggaccaccaa caccgtgccg agtcgcgcgc tggtcgaccc catgctcggc
     1561 gccagcgcgg tggcgttgga tccgcagagc ggacgcctgt atgtggcggg ttttgacgcg
     1621 aatatgatcc tggtgttcga cgatccccaa tcgtttgcga ccggccagat cgctccggat
     1681 cgaattcttg ccggaccatc cacccggctc gaccaccccg tggcgttgtt gtttcgtcgc
     1741 caagatgcgt ccgccaacgg atcgttgtac gtggtgaacc aatccagcca ttccgtctcg
     1801 gtgttcttgg aaggggacgg cgcggcggct ccgctgttgg cgggagatcg ggcgccggcg
     1861 cgctacatcg gaccgccgga cggcactgat ccgttctccg tgaccgcgaa cgttccccag
     1921 atggtcttcc ccacgggcct gactatcgat cccggcgtgg cggccgattt gacggacgat
     1981 atcctgtacg tctctaaccg ggacgccgag tccttccagg atctggtcgg tcggcggatc
     2041 gtggcgtttc aggatgcaaa cacggtgaac ggcgcggtgg ctccgacgtg gaaagtcgaa
     2101 ggcgatcgcc cgccgacggg atcggcaaag gccacggata agaccacgct gaaacgtccg
     2161 gcgggattgt ggatgatccc ggatccgctc ccgaacgatg ggacgccgga tgaccggctg
     2221 gtggtggcga accgggacgg caagagcgtg ctgattttcc gcgggctccg agcgctggtg
     2281 gcgcaggccg ccgcgaaccc gggggcgccg ccggaggaca accgcgcacc cgcgtggacg
     2341 atcaaacatt tcaagctggt ggcgcccttc ggggtggtgt tcgaacaagc cagccgccag
     2401 ctgtacgtgt cggatatcgc cggccggatc ctggcatttg atctcaccga cctggatacg
     2461 gatcttgccg acctggatat cgacaccccg gagcccgcgc tcaccccgcg cgtggtcgaa
     2521 ggggcgagca cgggcttggc cgcgccgctc ggcctggccc ttgatccgca aaactaacgc
     2581 ccgctacaat tcatcctgtg atgtcggaat gccgttccag gggcaggcga ctgcccgtcg
     2641 cgttgtccgt cgctctcgcc gcggcgcttt ccgggtgcgc ggggccggcc ccgttgcgcg
     2701 aggtgcgctc tccggagccg ccgggtgcga gccgcgcgct cgatcaatgc gccgaacggt
     2761 gcttcgcgga ggcgaaagcc gcgctcgcgg ccggtgacgt cgccaccgct caggaccggt
     2821 tgcggggcgt ccgtcgcgcc gcccccaaca cgcgatgggc gggtcgcgcc gccctgcttc
     2881 tggcccgggt ggacgcggag cgcgaccccg aggccgggct ccgttatgcc ctgcaggctg
     2941 cggcggatct gccggaactc ggagaccacg cgttagccgt cgccgcggac gcggcgcagc
     3001 acggaggccg accggtccaa gcggccggct acctgcacga actcgcccgc cgctacccgc
     3061 aatcatcact cgtgcccacc gcgctgtggt cggccgccgc gttgtgggcc ccgctggacg
     3121 gtcggcaagg tgaggccatc gcggcgctga ccgccctctc cacggatttt ccgtcggacc
     3181 cgcgcgtccc ggaggcgctg gcgaaaatcg tctcgctggc cgcgccttcg ggccggcgcg
     3241 aggaggccgc gctggcctgt cgacgtttgg tggtggactt ccccgcgtcg ccggacgccg
     3301 cgtcagtggc cgagtcctgc ctgaaggcgc ttcgcgatga cggggcgccg ctcacgttct
     3361 cggagctgcg ccgtcgggcc gaggggctcg cccgcggcgc aaaattttcg gaggcgctgg
     3421 acgtctggca ggacctgaag cggtccgcgc c
//
LOCUS       DBNGTT010000117         4668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1557044, whole genome shotgun sequence.
ACCESSION   DBNGTT010000117 DBNGTT010000000
VERSION     DBNGTT010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1557044"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="AC16BC_02685"
     CDS             <1..261
                     /locus_tag="AC16BC_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3240552.1"
                     /translation="PRVAQLVRPGGGIIALIKPQFEVGREHVGSGGIVRDSAQRARAV
                     QEVRIAAEALGLRCEGTIPSPIEGRDGNQEYLIALAAVARDA"
     gene            293..1432
                     /locus_tag="AC16BC_02690"
     CDS             293..1432
                     /locus_tag="AC16BC_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase
                     domain-containing protein"
                     /protein_id="HIR3240553.1"
                     /translation="MRGASVVVWGVLLGLTVGCTLPSTFHAIRRELAALPAGSAVPDP
                     PGLSRYKGVIHVHTRLSHDSRGEESEIARAAKSAGLDFVMLTDHNSPAIFEPGSRSEQ
                     DGVLFIRGAEIGCEGRYILAVGLQRSIDSQKMTCADVTAAVTEQGGVPFAAHPGKSTQ
                     WDDPSLAGVEVWDLYDEAKVDRWRYLAWAPDILVWYGTYPDEILSRIIRRPDQALAAF
                     DAQTVRRRLTAIGTPDAHQNIRIPGRQLDPYPLAFRLVPVYLLAEDGSRDALLGALRR
                     GRAYFAFEVFKPAPSFTFRAVDAAGAVWSMGDEVSQSPGLTVEVFAPSRGRITIVRDG
                     RVLSRIEGTYASAPVEGPAVYRAEVEISVQGRWRSWIFSNPIYVR"
     gene            1399..2763
                     /locus_tag="AC16BC_02695"
     CDS             1399..2763
                     /locus_tag="AC16BC_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HIR3240554.1"
                     /translation="MDFFKSHLRPLIAAAWLVGFTTACLPIDRTPVDATPDLTAATAQ
                     ADRIAVPAEAAIHAGAARVEMTPSGVPPLAGYGNRLGRPARGVHDPLYARALALRAGD
                     RTIVLVSLDLLAVTDDMVHAVEARIRREVSLSDDSLILTATHTHSSFGAVARRLWESF
                     AAGEYDEPMFRFLVERAAAAALGALEQLAPAQIAWGETSAPERIANRMIPGGPTDPAV
                     PFLSVARPNGDPVATLVNFAAHATVLKADNFLLSADYPGAVASALEQQGGVALFTAGA
                     VADQTGVPPAASDRFAAMDAMGADLAARVAATRRALPATAWVDHAAIDAWRLAIPLPA
                     PQLKVSPHRRLPKAVGDLLFDRRTTLTVAVVGEGLVIAVPCDLSATIGARWKAEAARL
                     GRRAVVVGFADDYVGYVIPAEYYETSHYEARMSFNGPYMDQYLTGVVTRALSRLGPLH
                     RASS"
     gene            2760..4094
                     /locus_tag="AC16BC_02700"
     CDS             2760..4094
                     /locus_tag="AC16BC_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240555.1"
                     /translation="MISRRALLLLAVAAAGCRPRLSMPGLNQHTPFSGAARAADGSLL
                     AKALEFEVAMADHLTPDGLVITERPGTPVPNDPLNAQDSAIWTGCYVAAEAFRWTATH
                     DPGAKDRLDHGLQALHLLQDVTGVPGVLGRMAKQADHAMPGERPTWRQGAGAWRAYRW
                     MGDVSVDQYAGVFFGYAAAFEAVGDADQRAAIAERVARLVDRLLAHAWRIVDETGRPT
                     KHSDLSGGRFTEPLNALLALSFAKVAAVTTGEARFEREYAALLERGYGRAAVRARDPW
                     WEYLFGVNHSDNNLAYLGLYPLLRLEPDPARRALFLRALRRVWGVVRAEVNPFLSFET
                     RALEDPAWRDDDAVARAVDTLWRFPFPKRDEPRRNSARRDICRSWFDDRHGDPQSCSP
                     LPIDQRPRSSFEWASNPYRLDRRGDPRTSLAGVDYLLAYWMGRRHGPLTDAQ"
     gene            4084..>4668
                     /locus_tag="AC16BC_02705"
     CDS             4084..>4668
                     /locus_tag="AC16BC_02705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3240556.1"
                     /translation="MRSNRSVPRASTGLLIVLLLAGCAGAIPPTRSAGPIPERETALF
                     APPAEDLHPRVDPPKRRGTYSVQRLSFDSVLRAPGLPPVEAFLYLPNEGRRFPLVIIL
                     PITRGDFFSTEFAVYFAERGLASIRFRSQGDIGRLYGDPASLSFFRDLLRARVIDTRR
                     VLDWLSRRQEIDVERVALVGFSHGAIVGSLVTAVD"
BASE COUNT          666 a         1544 c         1606 g          852 t
ORIGIN      
        1 ccgcgcgtcg cgcaactcgt ccgtcccggg ggggggatca tcgccttgat caagccgcaa
       61 ttcgaggtgg gacgcgagca cgtgggttcg ggagggatcg tgcgggactc ggcgcagcgc
      121 gcccgcgcgg tgcaggaggt gaggattgcg gccgaggcgc tgggtctccg atgcgagggg
      181 actatcccgt cgccgatcga ggggcgcgac ggcaatcagg aatacttgat cgcgcttgcg
      241 gcggtggctc gcgacgcttg atcgctccac aacgttccat tactatttcg gtatgcgcgg
      301 tgcatccgtg gtcgtgtggg gcgttctcct cggcctcacg gtgggatgta ccctcccgtc
      361 gacgttccac gcgatccgtc gagaactcgc cgccctgccc gcgggttccg cagtgccgga
      421 ccctcccggc ctctcacgat ataaaggcgt gatccacgtc cacacgcggt tgtcgcacga
      481 cagtcgtgga gaagaatcgg agatcgctcg cgcggcgaaa tcagcgggcc tcgacttcgt
      541 catgctcacc gaccacaaca gtccggccat cttcgaaccg gggtcgcgca gcgagcagga
      601 cggcgtgttg ttcatccgag gcgctgaaat tgggtgcgaa ggccgctaca ttctggcggt
      661 cgggttgcag cgctccatcg acagtcagaa gatgacgtgc gcggacgtga ccgcggccgt
      721 gaccgaacag ggaggagtcc cgtttgccgc gcaccccggg aagtcgaccc agtgggacga
      781 tccttcgctg gccggagtgg aggtctggga tctctacgac gaagccaagg ttgatcggtg
      841 gcgatatctc gcgtgggcgc ccgatatcct ggtctggtac ggcacctatc ccgatgaaat
      901 cctgtcgcgc atcattcgac gcccggatca ggcgctcgcc gcgttcgacg cgcaaaccgt
      961 ccgccgccgt ttgaccgcga tcgggacgcc cgacgcacac cagaatatcc ggattcccgg
     1021 ccggcagctg gacccctatc cattggcgtt ccgtctcgta cccgtctacc tgttggccga
     1081 ggacgggtcg cgcgatgcgc tgctgggggc actccgtcgc ggccgcgcct attttgcgtt
     1141 cgaggtgttc aagcccgctc cgagctttac gttccgggcg gttgacgccg cgggggccgt
     1201 gtggagcatg ggggacgagg tttcgcagtc accggggctc acggtcgagg ttttcgcgcc
     1261 gtcccggggg cggatcacga ttgtgcggga cgggcgggtc ttgagccgaa tcgaagggac
     1321 gtatgcttcg gcgccggtcg agggccccgc ggtgtaccgc gcggaggttg aaatttcggt
     1381 gcaaggacga tggcgttcat ggattttttc aaatcccatt tacgtccgtt aatcgcggcg
     1441 gcgtggctcg tgggctttac cacggcttgc ctgcctattg accgcacgcc cgtggatgcg
     1501 acaccggact tgaccgccgc gacggcgcag gcggatcgca tcgctgttcc cgccgaggcg
     1561 gccatccacg cgggcgcggc gcgggtagag atgacgccgt ccggcgttcc cccgctcgcg
     1621 ggatacggca atcgcctcgg ccggcccgcg cgcggcgtgc acgatcccct gtacgcccga
     1681 gccctggcgt tgcgggctgg cgaccgaacg atcgtactgg tgtcgctcga cttgttggcc
     1741 gtcaccgacg acatggtcca cgcggtggag gcgcgcatcc ggcgcgaagt ttctctgtcc
     1801 gacgacagct tgatcctcac ggcgacccac acgcattcca gtttcggcgc ggtcgcgcgc
     1861 cgactgtggg agagtttcgc tgcgggtgaa tacgatgaac ccatgtttcg ttttctggtc
     1921 gaacgcgcgg ccgcggccgc cctcggcgcg ctcgaacagc tggcgccggc gcagatcgcg
     1981 tggggggaaa catccgcgcc cgaacggatt gccaatcgga tgatcccggg agggccgacc
     2041 gacccggccg ttccgttcct gagcgtggct cgacccaatg gcgatccggt cgcaaccctg
     2101 gtcaacttcg cggcccacgc caccgtgctc aaggcggaca acttcctgct ctcggccgac
     2161 taccccggcg ccgtggccag cgcgctcgaa cagcagggag gggtggccct gtttaccgcc
     2221 ggcgcggtcg ccgatcaaac cggcgtgcct cccgccgcga gcgatcggtt cgccgcgatg
     2281 gacgccatgg gcgcggacct ggcggctcgc gtcgccgcga cgcgccgcgc gcttcccgcg
     2341 accgcatggg tggatcacgc ggcgattgac gcctggcgcc tggcgatccc gttgccggct
     2401 ccgcaactca aggtctcgcc gcaccgccgt ctcccaaagg cggtgggcga tctcttgttc
     2461 gatcgccgga ccacgctcac ggtcgcggtc gtcggggagg gactggtcat cgccgtgccg
     2521 tgcgacctgt cggcgacgat cggcgcgcga tggaaggccg aggccgcgcg gctgggacga
     2581 cgagccgtgg tcgtgggatt tgccgacgac tacgtggggt acgtgatccc cgccgagtac
     2641 tatgagacgt cgcactatga ggcgaggatg tctttcaacg ggccgtacat ggaccagtat
     2701 ctgaccggcg tggtgacccg cgcgctgagc cgtctcggtc cgcttcaccg ggcgtcgtcg
     2761 tgatctctcg acgcgccctg ctgctcctgg ccgtggccgc ggcgggctgt cgtccccggc
     2821 tcagtatgcc cggtttgaac cagcacaccc cgttctcggg agcggcccgc gcggcggacg
     2881 gctcgttact cgccaaggcg ctggaattcg aggtcgccat ggcggatcac ttgacgcccg
     2941 acggtctggt catcaccgaa cggcccggga cccccgtgcc caatgatccg ctcaacgcgc
     3001 aggacagtgc catttggacc ggatgttatg tggccgcgga agcctttcgc tggaccgcca
     3061 cccatgatcc cggcgccaaa gaccggctcg atcacggtct ccaggcgctg cacttgttgc
     3121 aagacgtgac cggggttcct ggagtcctgg gccggatggc caagcaggcg gatcatgcga
     3181 tgcccggcga gcgtcccacc tggcgtcagg gcgcgggggc gtggcgcgcg taccgatgga
     3241 tgggcgacgt gagcgtggat cagtacgccg gcgtgttctt cggatacgcc gccgcgttcg
     3301 aggccgtggg cgacgcggat caacgggcgg cgatcgccga gcgcgtggcc cgactcgtcg
     3361 atcgactgct cgcgcatgcc tggcggatcg tggatgaaac cgggcgcccc accaaacaca
     3421 gcgatctgtc cggcggtcgg ttcaccgaac cgctcaatgc cttgttggcg ctcagctttg
     3481 cgaaggtggc ggcggtgacg accggtgagg cgcgcttcga gcgcgagtac gccgctctgc
     3541 tcgaacgcgg atacggccgc gccgcggtgc gcgcgagaga cccttggtgg gagtacctct
     3601 tcggcgtcaa tcactcggac aacaatttgg cgtatctcgg actctacccc ctcctgcgac
     3661 tggaacccga ccccgcgcgg cgcgcgctgt tccttcgcgc gttgcgacgc gtgtgggggg
     3721 tcgttcgcgc cgaggtgaat ccgtttttgt cgttcgaaac ccgcgcgctc gaggacccgg
     3781 cctggcggga cgacgatgcg gtcgcccgtg ccgtggacac gctgtggcgc tttccgtttc
     3841 ccaagcgtga tgagccgcgc cgcaacagcg cgcggcgcga tatctgccgt tcgtggttcg
     3901 atgatcggca cggagatccc cagtcgtgtt cgccgttgcc gattgaccag cggccacgct
     3961 cgtcgtttga gtgggcgtcg aacccgtatc gcctggaccg gcggggcgat ccgcggacgt
     4021 cgctggccgg cgtggactac ctgctggcct actggatggg gcgccgccac gggccgttga
     4081 ccgatgcgca gtaaccggtc ggtcccgcgc gcgtcgacag ggttgctgat cgttctcctc
     4141 ttggccggct gcgccggggc gatccccccg acgcgttcgg cgggaccgat cccggagcgc
     4201 gaaaccgcgc tcttcgcgcc tccggccgag gaccttcacc cacgggtcga tcctcccaag
     4261 cgccgcggca cgtattcggt ccagcgactc tcgttcgact cggtcttgcg cgcaccgggg
     4321 cttccgccgg tcgaggcgtt cttgtatctc ccgaacgagg gccgccggtt tccgttggtg
     4381 atcatcctcc ccattactcg gggcgatttt ttctccacgg agttcgccgt gtatttcgcc
     4441 gaacgcgggt tggcgagtat tcgctttcgc agccaaggcg atataggacg gctttacggg
     4501 gacccggcca gtctctcgtt ctttcgcgac ctgctcaggg cccgagtgat cgacacccgg
     4561 cgcgtgctgg actggctttc acgacgccag gagatcgacg tcgagcgcgt cgcgctggtg
     4621 ggtttttccc acggagccat cgtcggcagc ctggtcacgg ccgttgat
//
LOCUS       DBNGTT010000118         9025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2068937, whole genome shotgun sequence.
ACCESSION   DBNGTT010000118 DBNGTT010000000
VERSION     DBNGTT010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2068937"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..928)
                     /gene="rfbD"
                     /locus_tag="AC16BC_02710"
     CDS             complement(92..928)
                     /gene="rfbD"
                     /locus_tag="AC16BC_02710"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR3240557.1"
                     /translation="MRIFVTGATGQLGLALQRVLAAHEVTAPPESESDITRTTIVDRI
                     TAARPEAVIHAAAYTDVDGAEANPDRAYAVNAEGSRLVAQAAAKAGARLIAISTDYVY
                     DGAKTAPYREDDPVAPLGVYGASKLAGEREALAAKPDAVILRTAWLYGEGKNFVRTIL
                     RLAGERDELRIVSDQIGSPTWAEDLAVAVRALLAVRASGIYHAVNSGACSWHEFAVAI
                     LQSAGYERRVVPILTAELGRPAKRPARSVLDCSKLAALGIRMRPWRDALSDYLRTPAA
                     RP"
     gene            complement(971..1978)
                     /gene="rfbB"
                     /locus_tag="AC16BC_02715"
     CDS             complement(971..1978)
                     /gene="rfbB"
                     /locus_tag="AC16BC_02715"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR3240558.1"
                     /translation="MRLLVTGGCGFIGSNFIRHVLAAHPEDSVVNLDKLTYAGNPENV
                     KDAARDPRYRFIQGDICDPEVVDRAAKDVDAIVNFAAETHVDRSLFDPGSFIKTDVDG
                     TRVLLEAARRYRHQRYVQISTDEVYGSTLGAPFRETDPLTPSSPYAASKAGGDLMALA
                     YHTTYGVPVIITRASNNFGPYQYPEKLIPLFITNAIDDQPLPLYGDGLNVRDWLAVED
                     HCEAVDAVLRRGAVGEIYNIGGGNERTNLEITDLILAGLNKPKSLIKPVADRPGHDRR
                     YAISAEKLAALGFRPRRRFDDAIRATIDWYRTHESWWRPIKSGEFRTYYEKQYRAIAL
                     S"
     gene            complement(1975..3054)
                     /locus_tag="AC16BC_02720"
     CDS             complement(1975..3054)
                     /locus_tag="AC16BC_02720"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HIR3240559.1"
                     /translation="MKALITAGGKGTRLRPITHTSNKHLIPIANKPMIAYALEAVAEA
                     GIREVGIVINPDTGEEIRQALGGGEAWGLKLTYVMQDAPRGLAHVVRVAQDFIGREPF
                     VFYLGDNVIVGGIRQFIDEFSRDGDHCRLVFARVRDPERFGVPELRNGKLVGVEEKPA
                     HPKSDFAVTGIYVYDHHIFEAVNAIAPSPRGELEISDAHQYLLEHGHRVGYSEITGWW
                     KDTGKPEDLLEANRLALDKVREQPEPFRPGQVDAKSDIAGKVILEQGATVIGSQIRGP
                     AVIGARTVIENSYVGPFTSIGADCVLRNSEVEYSIVLERGRIIDADIRIERSLLGREV
                     EILRCTSKPMTQKFIVGDQSKIELT"
     gene            complement(3137..3886)
                     /locus_tag="AC16BC_02725"
     CDS             complement(3137..3886)
                     /locus_tag="AC16BC_02725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3240560.1"
                     /translation="MAIEKERLSPDAAVPRWVWLEHLARYDFGARFAAGKVVLDCACG
                     AGIGSETFARAGAASVAAFDVSREEVDRARARTALPNLTFQHGSALALPVASHSIDVY
                     ISFETIEHIDEDRAFLREAQRVLKTDGVFVCSTPNRAVTNPGASLAHRPWNRFHVREY
                     SRDEFVALLREFFPRVELYGQNPKPAWLAQSVEGLGRILPMHGAVRLNQVMKLPQLVV
                     DSQARHAVRALEPGCAYEYLVAVCRGSGAAP"
     gene            complement(3921..4856)
                     /locus_tag="AC16BC_02730"
     CDS             complement(3921..4856)
                     /locus_tag="AC16BC_02730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3240561.1"
                     /translation="MYRGRSIGVVIPAYNVERSIRKVVEGLPSWVDRVIVVDDASRDG
                     TLKEAQSIVADNLIVLHHEVNQGVGGATMTGFRRALEEGVKVAVKMDGDGQMDPAHLP
                     ALLDAVTVQGYDSSKGNRFLHTTELSAMPRHRLLGNFVLTFLTKLTSGYWNVFDPQNG
                     YLAIRREPLSRLSFDRIAKRFFFENDLLIHLNILNYRVKDVPIPAIYGDEVSNLKVSR
                     VLGSFPLYLFRRFWYRIYQKYVLRDFSPIAVFLFLGLSFFLWGLGFGFYTWWRSAMTG
                     HFASTGTVMLSVLPFLIGFELILQAVILDIQATPK"
     gene            complement(4862..5884)
                     /locus_tag="AC16BC_02735"
     CDS             complement(4862..5884)
                     /locus_tag="AC16BC_02735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3240562.1"
                     /translation="MSGSGAPPLASVIVVNWNGESYLAECVDSLLAQTYPRLEIIVVD
                     NGSTDGSVRLLTKRYGAKIRLVLSPANLGFTGGNNLGMAAAGGAYVLLLNNDAVADPE
                     WVHALVRDAEADSRIGMCASKIVSYDDPSVIDSAGLLLARDGLARGRGRLARDESRFS
                     RPEDVLMPSGCAGLYRRAMLDEVGVFDERFFMYCDDVDLGLRGRVAGWRCRYVPSAVV
                     RHRYSLSAGKYSPRKIFLVERNRVWVMLKSFPWTLIALSLPWTALRLWWHAYAAWRGR
                     GGAGRAAEHVSAATLVATVLRAYAAALGGAFAALRQRRARASVVEFARWLRYDGVSAR
                     DVAMTE"
     gene            complement(5881..7992)
                     /locus_tag="AC16BC_02740"
     CDS             complement(5881..7992)
                     /locus_tag="AC16BC_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240563.1"
                     /translation="MAADVIPARTDWRRLAAAMVLALPLVAAVYANSLRNPFQFDDRH
                     AIVENPALQTSAVDWSWWGARAGTIGAGHYRPVTFLSYAFNLRVGGLDPFGFHLVNVG
                     LHWIAVATLMGALWLLGGRAGPAIVGGLVFAVTPANSEAVNYLAARSSLLVGLWSVTA
                     VAAFALMRRCQAQARPRSTLAAGAVAVVALGLGLASKETAVTVPLLWICYDVGWSRRI
                     GRGAVLRPYAVVAVLAVGYFAATGYWRALWTVLSGAQTGERAVWPNLWSQLAAFPLHA
                     TVFAWPFSLTVLHDVPTVDSPWRLSVFVGLVIALVACAGGAHWLVRASDARRPAGFLL
                     LWWVIALVPLMLYPLHLMFQEHRDYFSWMALAGLAGLGLSAVWDHAARRPAGRWALGA
                     AVVAVLAIWTVSTVERNRVWSDAMRLWSDAATKSPENAVAHVNLGTEYARRGDIDRAL
                     AEYRDVIRLQPDYGLAYHNVGLLYLGRGDYAEARPAFERAAELTPDAAEPLAALGTVY
                     TALGERALADDALLRAAAALDRRPHPVSVRLTVADALAKTGRVREAVTHYQAVLARER
                     GRPSFLSAKAYLGLGFLAERVGRREEALAAYTEALRIDPRLNDARYNMANVLLSAGRL
                     PDAMAAYERLLAAEPKFFQARFNLGRIYEQAGRLDDARGQYRDFLRDAPEGPLYAGAR
                     QYAASRVASVEPEPATGKLAP"
     gene            complement(8028..>9025)
                     /locus_tag="AC16BC_02745"
     CDS             complement(8028..>9025)
                     /locus_tag="AC16BC_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240564.1"
                     /translation="AAWINRAALWEDVVAKYPSSLMAHNGLGMVAFEAGRVEEARAWY
                     ERALRLSPGFTEGHKNLGVTFAAVGEWDQAIAAFERALAIDPGYVEARVNLGKVYERQ
                     GRWELALEAYDRILRDDPGNASVLARAAWLLGAAGRLPEAADRYARLRASGRADADAL
                     AAYGAVLVRLERWDEGERVLSELLARVPDSYPAQFNLAAALEGRGAWDRAADEYRRAA
                     ALNVADPDPLFRIGVLLSKQERWQQAAAAYDEALRRDSRHTPAHLNAGMVAERTGDVP
                     QALRHYDSVIAMTGGRPGDDGLRERAGEAAARLRRLAGGIEPPVAVRAGSPDGTK"
BASE COUNT         1545 a         3301 c         2812 g         1367 t
ORIGIN      
        1 cccgcggaac gccgagccac cccgcgatca ggaccaccgc gcaggcagcc ggtccgatcc
       61 tcgttctgag accgggcgcc gacatcgccg gtcagggccg agcggccggg gtgcggaggt
      121 agtcggacag cgcgtctcgc cacggccgca tccggatgcc cagcgcggcc agcttggaac
      181 agtccaggac cgagcgcgcg ggccgcttgg cgggacgccc caactccgcg gtcaagatgg
      241 ggaccacccg gcgctcgtac ccggccgact ggagaatcgc gaccgcgaac tcgtgccacg
      301 agcacgcgcc cgagttgacc gcgtggtaga tcccggacgc ccgaacggcc aacagcgcgc
      361 ggacggcgac cgccagatcc tcggcccacg tgggcgagcc gatttgatcg ctcacgatgc
      421 ggagttcgtc gcgctcgccg gccaatcgaa ggatcgtgcg cacgaaattt ttcccctccc
      481 cgtacaacca ggccgtgcgc agaatcaccg cgtcgggctt ggccgccagc gcctcgcgtt
      541 cgcccgcgag tttgctcgcg ccgtagacgc ccagcggcgc gaccgggtcg tcttcgcggt
      601 acggcgcggt cttggcgccg tcgtacacgt aatccgtgga gatcgcgatc aagcgtgcac
      661 cggctttggc cgcggcctgc gccaccagcc gcgacccttc ggcgttcacg gcgtaggccc
      721 ggtcgggatt cgcctcggcc ccatccacgt cggtgtaggc cgcggcgtgg atcaccgcct
      781 cgggcctggc ggccgtgatc cggtcgacga tcgtcgtccg cgtgatgtcg gattcggatt
      841 ccggcggcgc ggtcacctca tgtgcggcga gcactcgttg cagggcgagg cccaactgcc
      901 ccgtcgcgcc ggtgacgaag attcgcatgg tccctcgggc agtccgggaa atggcgcgcg
      961 gccgcgcggc ctacgataac gcgatcgccc ggtattgctt ctcgtagtac gtccgaaact
     1021 ctccggactt gatcggccgc caccaggact catgcgtccg gtaccagtcg atggtcgcgc
     1081 ggatcgcgtc gtcgaaccgc cggcgcggac ggaacccgag cgcggcgagc ttctccgcgc
     1141 tgatggcgta ccggcggtcg tgacccggcc ggtcggccac gggtttgatc aggctcttgg
     1201 gcttgttcag gcccgccagg atcaggtcgg tgatttccag attggtgcgc tcgttgccgc
     1261 cgccgatgtt gtagatctcc cccaccgcgc cgcgccgcaa caccgcgtcc acggcctcgc
     1321 aatgatcctc caccgcgagc cagtcgcgca cgttgagccc gtccccgtac agcggcagcg
     1381 gctgatcgtc gatggcgttg gtgatgaaca acgggatgag tttctccggg tactggtagg
     1441 gcccgaaatt gttcgaggcc cgcgtgatga tcacgggcac gccgtacgtg gtgtggtagg
     1501 ccagcgccat caaatcgccg ccggccttgg acgcggcgta ggggctgctc ggggtgagcg
     1561 ggtcggtctc ccgaaacggc gcgcccagcg tggagccgta gacctcgtcg gtggagatct
     1621 gaacgtaccg ctggtggcgg tagcgcctgg cggcctccag caacacccgc gtgccgtcca
     1681 cgtcggtctt gatgaacgag ccgggatcga acaaggaccg gtcaacgtgg gtttcggccg
     1741 cgaagttcac gatcgcgtcc acgtccttgg ccgcgcgatc cacgacctcg ggatcgcaga
     1801 tgtcgccttg gatgaagcgg tagcgcggat cgcgcgccgc gtctttcacg ttctcgggat
     1861 tgccggcgta ggtcagctta tcgaggttga cgaccgaatc ctcggggtgc gcggccagca
     1921 cgtgccggat gaaattgctg ccgatgaatc cgcagccgcc ggtgaccagc agtctcatgt
     1981 gagttcgatc ttgctttgat cgccgacgat gaacttctgg gtcatcggct tgctcgtgca
     2041 ccggaggatt tcgacctccc gacccagcag gctgcgttcg atgcggatat ccgcgtcgat
     2101 gatccgcccg cgttccagca cgatgctgta ttccacttcg ctgttgcgca ggacgcagtc
     2161 ggccccgatc gaggtgaacg gtcccacgta cgaattctcg atgaccgtcc gagccccgat
     2221 gaccgccggc ccgcgaatct ggctgccgat cacggtcgcg ccctgttcca ggatgacctt
     2281 tcccgcgatg tcggacttgg cgtccacttg cccgggccgg aacggttcgg gttgctcccg
     2341 aaccttgtcg agcgcgaggc ggttcgcttc caacagatct tcgggcttcc ccgtgtcctt
     2401 ccaccacccc gtgatctccg agtacccgac ccggtgtccg tgttccaaca ggtattggtg
     2461 ggcgtccgag atctccaact cgccccgcgg gctgggcgcg atggcgttga cggcctcgaa
     2521 gatgtggtga tcgtagacgt agatcccggt caccgcgaaa tcgcttttgg ggtgcgcggg
     2581 tttttcctcg accccgacca gcttgccgtt gcgcagttcc ggcacgccga agcgctccgg
     2641 atcgcgcacg cgcgcgaaca ccaaccggca gtgatcgccg tcccgcgaga actcgtcgat
     2701 gaactggcgg atgcctccca cgatcacgtt gtcgcccagg tagaacacga agggttcgcg
     2761 gccgatgaaa tcctgggcca cgcgcaccac atgcgccagg ccgcgcggcg cgtcctgcat
     2821 cacgtacgtg agcttgagtc cccacgcctc gcccccgccc agcgcctgac gaatctcctc
     2881 gccggtgtcg gggttgatca cgatgcccac ctcgcggatc ccggcctcgg ccaccgcctc
     2941 cagggcgtag gcgatcatgg gtttgttcgc gatcgggatc agatgtttgt tggacgtgtg
     3001 ggtgatggga cgcaggcggg tccccttgcc gcctgccgtg atgagggctt tcaccggttg
     3061 aactcgcgcg gcctcaaatt gcccgccatc ttagagagcg ctccaaggcc tgtcaagccg
     3121 gccgcgtcgc gggcggtcat ggcgccgcgc cgctcccacg gcacaccgcg accagatatt
     3181 cgtaagcgca acccggctcc aacgcccgca ccgcgtggcg cgcctgacta tcgacgacca
     3241 gttgcggcaa tttcatcacc tggttcagac ggaccgcccc gtgcatgggg aggatccggc
     3301 ccagcccctc gaccgactgg gccaaccagg ccggcttcgg attctggccg taaagttcca
     3361 cgcgcgggaa gaattcgcgc agcagcgcga cgaactcgtc ccgcgagtac tcccgaacgt
     3421 gaaaccggtt ccagggtcga tgcgcgagcg acgcgcccgg gttggtcacc gcccgattgg
     3481 gcgtggaaca cacgaacacc ccgtcggttt tgagcacccg ctgcgcctcc cgcaaaaacg
     3541 cgcgatcctc atcgatgtgc tcgatggttt cgaacgagat gtacacgtcg atcgaatgcg
     3601 acgcgaccgg caacgcgagg gcgctcccgt gctgaaaagt caggttcggc aacgcggtcc
     3661 tggcccgagc ccggtccacc tcttcccgcg acacgtcgaa cgcggccacc gacgccgcgc
     3721 cggccctggc aaaggtctcg gacccgatgc ccgcgccgca cgcgcagtcc aacacgacct
     3781 ttcccgccgc gaaccgcgcg ccgaagtcat accgggccag gtgttccagc cacacccacc
     3841 ggggcacggc cgcgtcgggg ctgaggcgtt ccttttcgat cgccatcagc cgcccgtcgt
     3901 caatcgtcgc gcgcggctcg tcatttgggc gtcgcttgaa tatcgaggat cacggcctgc
     3961 aatatcaact cgaacccgat cagaaagggc aacacgctga gcatcacggt gccggtggag
     4021 gcgaaatgcc ccgtcatcgc ggaacgccac cacgtgtaga acccgaaccc cagcccccag
     4081 agaaaaaagc tcagcccgag gaacaggaac accgcgatgg gcgagaaatc ccgcaacacg
     4141 tacttctgat aaatccggta ccagaatcgg cgaaagagat acaggggaaa cgagccgagc
     4201 acgcgcgaga ccttcagatt ggagacctcg tccccgtaga tcgcggggat cggcacatcc
     4261 ttgacgcgat agttcaggat gttgaggtga atcaacagat cgttctcgaa gaaaaaccgc
     4321 ttcgcgatcc gatcgaagct caggcgcgag agcggttcgc ggcggatcgc gaggtatccg
     4381 ttctgcgggt cgaacacgtt ccagtagccg gacgtcagct tggtcagaaa cgtcaacacg
     4441 aagttgccca acaaccggtg gcgaggcatc gccgagagtt cggtcgtgtg caggaagcgg
     4501 ttgcccttgg aggaatcgta gccttggacc gtcaccgcat cgagcaacgc gggcaggtgc
     4561 gccgggtcca tctgcccgtc cccgtccatt ttcaccgcga ccttgacacc ctcctccaac
     4621 gcgcggcgaa acccggtcat ggtcgcgccg cccacgccct ggttcacctc gtgatggagc
     4681 acgatcagat tgtcggcgac gatcgattgc gcctccttga gggttccgtc ccggctggcg
     4741 tcgtccacca cgataacccg gtccacccac gacgggaggc cctcgaccac cttccggatc
     4801 gagcgctcca cgttgtaggc ggggatgacc acgccgatgg atcgaccgcg atacatagtc
     4861 gctactccgt catcgccacg tcgcgcgctg aaactccatc atagcggagc cagcgcgcaa
     4921 actccaccac ggacgcgcgg gcccggcgct gtcggagcgc ggcaaacgcg ccgccgagcg
     4981 cggccgcgta tgcccgaagc acggtcgcga ccagcgtggc cgcggacacg tgctccgccg
     5041 cgcgaccggc cccgccgcgc ccgcgccacg ccgcgtaggc gtgccaccac aggcgcagcg
     5101 cggtccacgg caacgacagc gcgatcagcg tccagggaaa cgacttgagc atcacccaca
     5161 cccgattgcg ctccaccaga aagatcttgc gcggcgaata cttccccgcg gacagcgaat
     5221 accggtgccg caccaccgcg gagggcacgt accggcaccg ccagcccgcc acccgcccgc
     5281 gcaggcccag gtccacgtcg tcgcagtaca tgaagaaccg ctcgtcgaac acccccactt
     5341 catcgagcat ggcccggcga tacaacccgg cgcatccgct gggcatcaac acgtcttcgg
     5401 gccggctgaa ccggctctca tctcgcgcca ggcggccccg gccccgcgcc aacccgtcgc
     5461 gggcgagcaa cagccccgcg ctgtcgatca cggacggatc atcgtacgac acgatcttcg
     5521 aggcgcacat gccgatccgt gaatccgcct ccgcgtcccg caccagggcg tgcacccact
     5581 ccggatcggc caccgcgtca ttgttgagca gcagcacgta cgcgcccccc gccgccgcca
     5641 tccccagatt gttccctccg gtaaatccga ggttggccgg gctgagcacc agacgaatct
     5701 tcgcgccgta ccgtttggtg agcaatcgaa ccgatccgtc ggtcgagccg ttgtcgacca
     5761 cgatgatctc aagcctgggg taggtctggg ccaacaacga gtcgacgcac tcggcgagat
     5821 acgactcgcc gttccagttc accacgatca cggacgccag cggcggcgcg ccggacccgc
     5881 tcacggcgcc agcttccccg tcgcgggttc cggctcgacg gaggcgaccc gggacgccgc
     5941 atactgccgc gcgcccgcat acagcggccc ctccggcgca tcgcgcagga aatcgcggta
     6001 ttgtcctcgg gcgtcatcga gccgcccggc ctgctcgtaa atgcgcccca ggttgaaccg
     6061 ggcctgaaag aatttgggct ccgccgcgag caaccgttcg taggcggcca tcgcatccgg
     6121 gagtcgtccc gcggacaaca acacgttcgc catgttgtag cgcgcgtcgt tcaggcgagg
     6181 atcgattcgg agggcttcgg tgtaggccgc cagcgcctcc tcgcggcgtc ccacgcgctc
     6241 ggccaggaag cctaggccca gataggcctt ggccgagagg aacgacggcc ggcctcgttc
     6301 ccgcgccagc accgcctggt agtgcgtcac cgcctcgcgc acgcgcccgg tcttggccaa
     6361 tgcgtcggcc accgtgagac ggacgctgac cgggtgcggc cgccgatcca acgccgcggc
     6421 ggcgcgaagc agcgcgtcgt cggccaacgc ccgctcaccc agcgccgtat acaccgtccc
     6481 cagggccgcc aacggctcgg cggcgtccgg cgtcaactcc gcggcccgtt caaacgccgg
     6541 ccgcgcctcc gcgtaatctc cgcgccccaa gtacaacaac cccacgttgt ggtaggccag
     6601 gccgtagtcc ggttgcaggc gaatcacgtc gcgatactcg gccaacgccc gatcaatgtc
     6661 tcctcgtctc gcgtattcgg ttcccaagtt cacgtgcgcc accgcgttct ccggagactt
     6721 cgtcgccgcg tcgctccaca accgcatcgc gtcgctccac acccgattgc gctccaccgt
     6781 gctcaccgtc cagatcgcga ggaccgccac cacggcggcg cccaacgccc agcgcccggc
     6841 ggggcggcgc gcggcgtgat cccagaccgc gctcagcccc agtccagcca ggccggccag
     6901 cgccatccac gagaaatagt cccggtgctc ctggaacatg aggtgcaacg gatacagcat
     6961 caagggcacg agcgcgatta cccaccacag caacaagaat cccgcgggcc ggcgcgcgtc
     7021 cgacgcgcgg accagccagt gcgcgccgcc ggcgcacgcc acgagcgcga tcaccagccc
     7081 gacgaagacg ctcaggcgcc agggcgagtc gaccgtcggc acgtcgtgca ataccgtgag
     7141 tgaaaacggc cacgcaaaca ccgtcgcgtg cagcgggaac gccgccaact gactccagag
     7201 attcggccac accgcgcgtt ccccggtctg cgccccggac aacacggtcc agagcgcgcg
     7261 ccagtatccg gtcgcggcga aatacccgac cgccaggacc gccaccaccg cgtacggtcg
     7321 cagcaccgcc ccgcggccga tccgccggga ccagcccacg tcgtaacaga tccataacaa
     7381 cggcacggtg accgcggttt ccttgctcgc caagccgagc cccaacgcga caaccgcgac
     7441 ggcgccggcc gccagcgtgc tcctcggtcg cgcctgggcc tggcatcgcc gcatgagcgc
     7501 gaacgccgcc accgccgtga cgctccacag tcccacgagc agcgacgagc gggccgcaag
     7561 gtagttcacc gcctcgctgt tggccggtgt cacggcaaac accagcccgc ccacgatggc
     7621 cgggcccgcg cgacccccca gcagccagag cgcacccatc aaggtcgcga ccgcaatcca
     7681 gtgcagtccc acgttgacga gatgaaaccc gaacgggtcg agccctccga cccgaagatt
     7741 gaaggcgtac gagagaaacg tcaccggtcg gtagtgcccc gcgccgatgg ttccggcccg
     7801 tgccccccac catgaccaat ccacggccga cgtctgcaac gcggggttct cgacgatggc
     7861 gtgacgatca tcgaattgga agggattccg gagcgaattg gcgtacaccg ccgccaccag
     7921 cgggagcgcc aacaccatcg ccgccgcgag tcggcgccag tcggtacgcg ccgggatcac
     7981 atcagccgcc atgaccacct cccctcactt cgagaccacg cacgccctta tttcgttccg
     8041 tccggcgacc cggcgcggac agcaaccggg ggctcgattc cgccggcgag gcggcgcagg
     8101 cgagccgcgg cctcgccggc ccgctcccgg agaccgtcgt cgccgggacg cccgccggtc
     8161 atcgcgatca ccgaatcgta atgccgcagc gcctgcggca cgtcacccgt tcgttcggcg
     8221 accatccccg cgttcagatg cgcgggggtg tggcgggaat cccgccgcag cgcctcgtca
     8281 tacgcggccg ccgcctgttg ccaacgctct tgcttgctca gcaacacgcc gatccgaaac
     8341 agcggatcag ggtccgcgac gttcagcgcg gccgcccgtc ggtactcgtc ggccgcgcga
     8401 tcccacgctc cacgtccctc caacgccgcc gcaaggttga attgcgccgg gtatgaatcc
     8461 gggacccggg ccagcaattc cgacaacacc cgttcgccct cgtcccagcg ttccagccgg
     8521 accagcaccg ccccgtacgc cgccagcgcg tccgcatccg cccggccgct cgccctcaga
     8581 cgggcgtacc gatccgccgc ttcgggaagg cggccggccg cgccgagcaa ccacgcggcc
     8641 cgggccagca cggacgcgtt gcccgggtca tcgcggagga tgcggtcgta cgcctccagg
     8701 gccagttccc atcgcccttg gcgttcatac accttgccca ggttgacgcg cgcctcgaca
     8761 taccccggat cgatcgcgag cgcgcgctcg aacgcggcga tcgcctggtc ccactcgccg
     8821 accgcggcga acgtcacgcc gagattctta tggccttccg tgaatcccgg agacagccgg
     8881 agcgcgcgct cgtaccacgc ccgcgcctcc tccacccgtc ccgcttcaaa ggccaccatc
     8941 cccagaccgt tgtgcgccat caaactggag gggtatttgg ccacgacgtc ctcccacagc
     9001 gcggcgcggt tgatccacgc cgcgg
//
LOCUS       DBNGTT010000119         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2963071, whole genome shotgun sequence.
ACCESSION   DBNGTT010000119 DBNGTT010000000
VERSION     DBNGTT010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2963071"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="AC16BC_02750"
     CDS             <1..341
                     /locus_tag="AC16BC_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3240565.1"
                     /translation="ASYMESVPGARQYPQRIEDLLKDPRTSKRHLRTGYLDPITGSSF
                     HAIPCKDRIKGVVSASDAVPLKRDNFPAAYEQFRSAGTYQDWKFQYEPQTAQAGQPGQ
                     PKVAAIPIPC"
     gene            499..>2017
                     /locus_tag="AC16BC_02755"
     CDS             499..>2017
                     /locus_tag="AC16BC_02755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3240566.1"
                     /translation="MSRPPHTPIGIALGLILIAAAWPRLPLAVAATPPVHHELSVVLE
                     PEQGRLRVEDAVTLPRTGDTRALRFRLHSGWSAQALDAGVRLTRVEQPPDADRSAEIS
                     GDEYEVSIPATAKRFRVAYEGSIERSDDPGSHGALTTDVVFLDGGSLWYPVFDEELVT
                     FTLEVRAPAGWDVVSQGRRASRRKDGDSGVVRWTSPEPQDDIFLVGGPLTEYSRSAGK
                     VTAMALLRSPDPDLAKTYLDATARYLAMYSTLFGPYPYPKFAMVENAWETGYGMPSFT
                     LLGSSVIRFPFILTSSYPHEILHNWWGNGVFVDAAKGNWSEGLTAYLADHLMAEQRGA
                     GAEYRRATLQKYADYVTHGRDFPLAEFRSRHDPATEAVGYGKALMVFHMVRRQIGDDA
                     LIHGLRKFFKEFRFQRATFEDLDHALAAVAGRTTVMMSPWVEQAGAPALRVSNAEANP
                     LGDRYLLTALVEQTQPGAAYRLRVPVAVTLDGREQAYQTSFQLETKHRGLELIVPG"
BASE COUNT          340 a          623 c          689 g          365 t
ORIGIN      
        1 tcgcgagtta tatggagtcg gtgcccgggg cgcgacagta cccccagcgg atcgaggacc
       61 tgctcaagga cccgcggaca tccaagcggc atctccggac cgggtacctt gatccgatca
      121 cggggtcgtc gttccacgcg attccgtgta aggaccggat caagggcgtg gtcagcgcca
      181 gcgacgcggt gcctctgaag cgcgacaact tccccgcggc gtacgaacaa tttcgctccg
      241 ccggcacgta tcaagactgg aagtttcagt acgaaccgca gacggcacag gcgggtcagc
      301 ctgggcagcc gaaggtcgca gccattccga tcccctgttg attcggcacg atgagccggg
      361 ggcggttcca agtacggaag gcgctttcgg agccacgggt gagcgcattg ccttcccgta
      421 ttgagtagct tccctatccg ggtttcatcc gcgtcgaatc gcttgacgcc ggccgccctc
      481 ttgtgcagga atagcgccgt gtcgcgcccg cctcatacgc ctatcggcat tgccttgggc
      541 ttgattctga tcgccgcggc gtggccccgc ctgccgttag cggtcgcggc cacgccgccg
      601 gttcaccacg aattgtcggt cgtgctggag cctgaacagg gccgtctgcg cgttgaagac
      661 gcggtcacgc tgcctcggac cggagacaca cgggcgcttc ggttccggct gcactccggg
      721 tggagcgcgc aggcgcttga tgccggcgtg cggctgacgc gggtcgaaca gcctccggac
      781 gccgaccgct cggctgaaat ttccggcgat gagtacgagg tatccatccc ggccacggcg
      841 aagcggtttc gcgtggcgta cgaggggagc atcgaacggt cggatgatcc cgggtcgcac
      901 ggcgcgctga ctacggacgt ggttttcctc gatggcggca gtctgtggta cccggtgttc
      961 gacgaggagt tggtgacgtt cacgctggag gtgcgcgcgc ccgcgggttg ggacgtggtc
     1021 agccagggta ggcgggcgag tcgccgcaag gacggcgact ccggtgtggt gcgatggacg
     1081 tcgccggaac cccaggacga catcttcctg gtcggcgggc cgttgactga atacagccgt
     1141 tccgcgggca aggtcacggc catggcgttg ctgcggagcc ctgatcccga cctggccaag
     1201 acgtacctgg acgcgaccgc gcggtatctg gccatgtaca gcacgctgtt cgggccgtat
     1261 ccctatccca agttcgccat ggtggagaac gcgtgggaaa ccggctacgg catgccgtcg
     1321 ttcacgctgc tcggatcatc ggtgatccga ttcccgttca ttctcacgtc gtcgtacccg
     1381 cacgagatcc tgcacaactg gtggggcaac ggggtgttcg tggacgccgc caaaggcaac
     1441 tggtcggaag ggctgaccgc gtatctggcg gatcatttga tggctgagca gcggggagcc
     1501 ggcgcggaat accgccgggc cacgctccag aagtacgcgg actacgtcac gcacggccgg
     1561 gactttccgc tcgcggaatt tcgatcccgg catgatcccg cgaccgaggc ggtgggatac
     1621 ggcaaggcgc tcatggtctt tcatatggtg cgccggcaga tcggcgacga cgcgttgatc
     1681 cacggcctcc ggaaattttt caaagaattt cgctttcaac gggccacgtt cgaggatctg
     1741 gatcacgcgt tggcggccgt ggcagggcgg acgaccgtta tgatgtcgcc gtgggtcgag
     1801 caagcgggcg cgccggcgct ccgagtcagc aacgcggagg ccaatccgtt gggcgaccgg
     1861 tacctgctga ccgcgcttgt cgagcaaacc caaccagggg ctgcgtaccg cctgcgcgtg
     1921 ccggtcgccg tcacactcga tggacgcgag caggcgtacc agacgtcgtt tcaactcgaa
     1981 accaagcacc gtgggttgga gctgattgtg ccgggaa
//
LOCUS       DBNGTT010000120         1651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_408124, whole genome shotgun sequence.
ACCESSION   DBNGTT010000120 DBNGTT010000000
VERSION     DBNGTT010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_408124"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..390
                     /locus_tag="AC16BC_02760"
     CDS             148..390
                     /locus_tag="AC16BC_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240567.1"
                     /translation="MRLRFYVAMAVLVVGAAFFVDYLMGKLYVRYMILFSGAIFLWGM
                     VVGQRSEQAWMRRRVQRAAGATPRPPFGHSDEEVLR"
     gene            387..596
                     /locus_tag="AC16BC_02765"
     CDS             387..596
                     /locus_tag="AC16BC_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240568.1"
                     /translation="MKHLFYAFYVSFYAGAMFVAGLFAGQFMERRWAVKEREQAAEAA
                     RRADDQGLVLDKAPVALSNQSQLTR"
     gene            669..1574
                     /locus_tag="AC16BC_02770"
     CDS             669..1574
                     /locus_tag="AC16BC_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3240569.1"
                     /translation="MSPLLKWLYLAVVLVIVGILMVGIKRTGKVPGEESESSGYTPLA
                     VKIKAAEEQMKNAEGQLSTVTIDPEFDRMIRIPGGPFTMGDRMGGAIEQPERTITLSP
                     FWIDAYETTFAHYYAFMSATGHRKPRLAGYLAVDSSALPYLMNPYNPVVGVFWDDANA
                     YCIWKGKRLPSEVEWERAAKGPNQRLYPWGNDPIATNANLVDDADGFKYTSPVGAFRN
                     DRSPEGVFDLAGNAMEWTSDWFDERYYATMSDRDPLGPPNGNERVIRGASWNDSVSRA
                     KATTRFKMRPDYRDVTIGFRCAKSS"
BASE COUNT          274 a          491 c          542 g          344 t
ORIGIN      
        1 tatgtacgaa ttcggcttcg ggcacctcgt cttatatccc ctcggcattt tcgcgttgag
       61 cgcggtgttc tttctctggg gctaccgggt cggccgtcgt cgtgaacgca cgcggctcga
      121 tgtgccggtg ccgtcgccgg ggcgctgatg cggctgcggt tctacgtcgc catggccgtc
      181 ctggtggtgg gggccgcgtt cttcgtggac tacctgatgg ggaagttgta cgtgcggtac
      241 atgatcctct tttcaggcgc catttttctc tggggcatgg tggtggggca gcgatcggag
      301 caggcatgga tgcggcggcg cgtgcagcgc gcggccggag cgacgccccg tcctccgttc
      361 ggtcattcgg atgaggaggt tctgcggtga agcacctgtt ctacgcgttt tacgtctcgt
      421 tttacgcggg cgccatgttc gtggcgggat tgttcgccgg gcagttcatg gaacgtcgct
      481 gggccgtcaa agagcgcgag caggccgccg aggcggctcg tcgcgccgat gaccagggtc
      541 ttgtcctcga caaagccccg gtggcgttgt ccaaccagtc gcagttgacg cgataagccc
      601 tcgcgactcc ttccgcgcgg cgggcccgac atgaggtccg ccgccttgct ccgggacgat
      661 cactcgccat gagtcccctc ctgaaatggt tgtacctcgc ggtggtcctc gtgatcgtcg
      721 ggattctcat ggtcggcatc aaacggaccg ggaaggtccc gggcgaggag tccgagtcgt
      781 ccgggtacac gccgctggcg gtcaaaatca aggccgcgga agagcagatg aagaacgccg
      841 aggggcaact ctccacggtg accattgatc cggagttcga tcgaatgatc cggattcccg
      901 ggggcccgtt caccatgggt gaccggatgg gaggggcgat cgagcaaccg gagcgaacaa
      961 tcacgctgtc tcccttctgg atcgacgcct acgaaaccac gttcgcgcat tactacgcgt
     1021 tcatgtcggc caccggccac cggaaaccac gccttgccgg gtatcttgcg gtggacagca
     1081 gcgcgctgcc gtacttgatg aacccgtata atcccgtggt cggggtgttc tgggacgacg
     1141 ccaacgccta ttgcatctgg aaaggcaagc ggctcccgag cgaggtcgag tgggagcggg
     1201 ccgccaaagg tccgaatcag cggctgtacc cgtggggcaa cgacccgatc gccacgaacg
     1261 cgaatctggt cgatgacgcg gacgggttca agtacacgtc gccggtcggc gcgttccgaa
     1321 acgaccgcag ccccgaaggc gtgttcgatc tggccgggaa tgccatggaa tggacgtccg
     1381 attggttcga tgagcggtat tacgccacca tgtccgatcg cgatcccctg ggccctccaa
     1441 acgggaacga gcgggtgatt cgcggcgcgt cctggaatga ctcggtttcc cgggcaaagg
     1501 ccaccacccg attcaaaatg cggcccgatt atcgggacgt gaccatcggt tttcgctgtg
     1561 caaagtcctc gtaatcgttg cggttgagcc tcaagtatgc gttgagtcac ggttcggttt
     1621 ttttcgcttg actccctatt tcgccttccc t
//
LOCUS       DBNGTT010000121         2784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2324146, whole genome shotgun sequence.
ACCESSION   DBNGTT010000121 DBNGTT010000000
VERSION     DBNGTT010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2324146"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..262
                     /gene="rpsU"
                     /locus_tag="AC16BC_02775"
     CDS             71..262
                     /gene="rpsU"
                     /locus_tag="AC16BC_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3240570.1"
                     /translation="MKIVVSDNQVDKALRVLKKKLIKEGLLKEMKKREYYEKPSVKRK
                     RKEKESRKRLRKLQAKKTP"
     gene            270..1328
                     /locus_tag="AC16BC_02780"
     CDS             270..1328
                     /locus_tag="AC16BC_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240571.1"
                     /translation="MIARLIAAVFGLILSAAPSPSIATSFSPYPDDSLDGPASPVQIR
                     LRSIQVQLFRDDGDQLPDGNAIGGGSGTGRSSVLLRRAELGVTGGLTPRARYLVVADL
                     AASPVLRDAFIELGAGPQFQVRAGQLRLPFGIETQLPPHRLPAINRMLMTFLAEQPDE
                     IPGFLQEWDRGIEVLGEPISGPLNLSYAFAVVNGTGPNAPDSNDAKDLVGRVGLRLAG
                     LQVGTSWYRGRAPDSAGFDRARNRAGWDLEFSPNPLKALLIRGELIHGHDETAANRGW
                     YLLASYTIRDRWTPLVRIERWDPDLDTGADTITRTTLGLTLAAWDIAAFSLNYEYRRD
                     AAHPGVGNLALAQAQISF"
     gene            1540..1941
                     /gene="acpS"
                     /locus_tag="AC16BC_02785"
     CDS             1540..1941
                     /gene="acpS"
                     /locus_tag="AC16BC_02785"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR3240572.1"
                     /translation="MNGAIVGIGIDLVRISRVREALGRWADRFLSRVLSQEETAYCRA
                     RVDPAPHVAARFAAKEAAMKALGVGWAGASWREFEVVNTPSGQPLIRFSGRAAQRLAN
                     LGADTAFVSMTHDGDYAAAQVVLSRTAPSPL"
     gene            1938..>2784
                     /locus_tag="AC16BC_02790"
     CDS             1938..>2784
                     /locus_tag="AC16BC_02790"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR3240573.1"
                     /translation="MNIVTAEEMRRLDDATIVNYGLPVTILMEQAGMGVVREMERRFG
                     DLAGRSVVVLAGKGHNGGDGLVAARHLIQRGAHVRVFLTVPADAGRGESQLQTVIYRN
                     IGGRLLDPSSYDRTVFRAALDQADLVIDALVGTGLSAPITGLAADLVADVNASGKPVT
                     AVDIPSGIGADTGEALGAAVRATLTVTFALPKRGHFLPPGSDHTGELRVVDIGIPQRA
                     IDQAALPLSLFTETAAAACVPARPREGHKGTFGHVLVVAGSLGKSGAAMLTARAALRT
                     GAGLTT"
BASE COUNT          465 a          987 c          893 g          439 t
ORIGIN      
        1 ccgcggaagg ttccggtatc ctgcgccgtc gttgttgagg agccgaccac ggaggttgtg
       61 acaccaggca atgaagatcg tcgtcagcga taatcaggtc gacaaggccc tccgggtcct
      121 caagaaaaaa ctcatcaaag agggcctcct caaagagatg aagaagcgcg agtactatga
      181 aaagccgagc gtgaaacgca aacgcaagga aaaggaatcg cgcaaacgac tccgcaagct
      241 ccaagccaag aagaccccct aacctcccca tgatcgcgcg gctgatcgcc gcagtgttcg
      301 ggctgatcct gtcggctgct ccaagccctt cgatcgccac ctcgttttca ccctaccccg
      361 acgactcgct cgacggcccg gccagcccgg tgcagatccg tctgcgcagc atccaggtcc
      421 agctctttcg cgatgacggc gaccagctcc cggacggcaa cgccatcggc ggcggatcag
      481 gcaccggccg cagcagcgtc ctactgaggc gggcggagct cggcgtcacc ggcgggctca
      541 cgccgcgcgc gcggtatctg gtggtcgcgg atctggccgc atccccggtc ctgcgtgacg
      601 cgttcatcga gctcggcgcc ggtccccagt ttcaggtacg cgccggccaa ctccgcctcc
      661 ccttcgggat cgaaacccaa ctgccgcccc atcgcctccc cgccatcaac cgcatgctca
      721 tgacgttcct cgccgagcaa cccgacgaga tccccggatt tctccaggaa tgggatcgcg
      781 gaatcgaggt gttgggcgag ccgatcagcg ggccgctgaa cctgtcttac gcgttcgccg
      841 tggtcaacgg caccggcccc aacgccccgg actccaacga cgccaaggac ttggtcggtc
      901 gcgtcggcct gcgcctggcg gggctccagg tcggcacctc gtggtaccgt ggccgcgccc
      961 cggactccgc cgggttcgat cgcgcccgca accgcgccgg gtgggatctg gaattcagtc
     1021 ccaatccgct caaagcgctc ctgatccgcg gcgagctgat ccatggacac gacgagaccg
     1081 cggccaaccg aggctggtac ctcttggcca gctacaccat ccgcgatcgc tggacccccc
     1141 tggttcgaat cgagcgctgg gacccggacc tcgacaccgg cgcggacacg atcacccgaa
     1201 cgaccctcgg cctgaccctc gccgcctggg acatcgccgc cttctccctc aactacgaat
     1261 accgccgcga cgccgcccac cccggcgtgg gcaacctcgc cctcgcccaa gcccaaatca
     1321 gtttttaagc gcattgttct gaaaccgctc tccctcttct ggagagcgaa gcctgtcgag
     1381 gagcggggca gatgccaggc gctgcgagca ccggagcgga gcgtactccg tgtgtacgtg
     1441 agcaccggat gcgcagcagc aacgccgcag atgccccctc atcggcaggc ttccccgcgg
     1501 cttgatttgg ggatagcggc gctgccataa tccgccccga tgaacggagc cattgtgggg
     1561 atcggcatcg acttggtcag gatttcccgc gtgcgcgagg ccttgggccg gtgggcggat
     1621 cgcttcctgt cccgcgtgtt gtcccaggag gaaaccgcgt actgccgggc ccgcgtcgac
     1681 cccgcgccgc acgtcgccgc gcgcttcgcg gccaaagaag cggccatgaa ggccctgggc
     1741 gtgggatggg ccggcgcctc gtggcgcgag ttcgaagtgg tcaacacccc gtccggccaa
     1801 cccctgattc gcttttccgg acgcgcggcc caacgcctgg cgaatcttgg agcggacacc
     1861 gcgtttgtca gcatgaccca cgacggggat tatgccgcgg cgcaggtcgt cctcagtcgc
     1921 accgcgccgt cgccgctatg aacatcgtca ccgcggaaga aatgcgtcgc ctcgacgacg
     1981 ccacgatcgt caactacggc ctccccgtga ccatcctcat ggagcaagcg gggatgggag
     2041 tggtgcgcga aatggaacgc cgattcggcg atctcgcggg ccgctcggtg gtggtgctcg
     2101 cgggcaaggg gcacaacgga ggcgacggcc tggtggcggc tcgccacctc attcagcgcg
     2161 gcgcgcacgt tcgcgtgttc ctgaccgttc ccgccgacgc gggccgcgga gagtcccaac
     2221 tccaaaccgt catctatcgc aacatcggag gacgactgct cgatccttcg tcgtacgatc
     2281 ggaccgtgtt tcgcgccgcg ctcgaccagg ccgatctggt gatcgacgct ttggtcggca
     2341 ccggcctgtc cgccccgatc acgggcctgg ccgcggacct ggtggcggat gtcaacgcgt
     2401 cgggcaaacc cgtgaccgcg gtcgacatcc cgtcggggat cggcgccgat accggcgagg
     2461 cgttgggcgc ggccgtgcgc gcgacgctga cggtcacctt tgcattgccc aagcgcggcc
     2521 acttcctgcc cccggggtcg gaccacaccg gcgagttgcg ggtggtggac atcgggattc
     2581 cccaacgggc gatcgaccag gccgcgctgc ccttgtccct gtttaccgag accgccgcgg
     2641 cggcctgcgt gcccgcgcgt ccgcgcgagg ggcacaaggg cacgttcggg cacgtgttgg
     2701 tggtcgcggg ttccctgggc aagagcggag cggcgatgct gaccgcgcgc gccgcgctga
     2761 gaaccggggc cgggctgacc acgc
//
LOCUS       DBNGTT010000122         3401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1685390, whole genome shotgun sequence.
ACCESSION   DBNGTT010000122 DBNGTT010000000
VERSION     DBNGTT010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1685390"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="lysS"
                     /locus_tag="AC16BC_02795"
     CDS             complement(<1..1121)
                     /gene="lysS"
                     /locus_tag="AC16BC_02795"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR3240574.1"
                     /translation="MPHQMDDLNDQTRSRLAKLDTLKSLGIAPFGRRFVPDDTAAGVH
                     QRFGATPRERLDSEVPEACVAGRVVGLRRFGKAAFAHLQDASGKIQVYLKRDALSPDD
                     FAVVEQLDLGDFIGVRGRVFRTKTDELTVEGKRIVHLSKALRPLPEKWHGLTDVEIRY
                     RQRYLDLIANPEVKDTFVRRSRIIDGIRKFFNGRGFLEVETPMMQGMAGGAAARPFIT
                     HHNALSTDLYLRIAPELYLKRLIVGGFERVYEINRNFRNEGISTIHNPEFTMLEFYLA
                     YADVTDLLPMTEELITSIAMDVCGTLELTYQGKAIDLRPPWKRIGMLDAIAERHGVDR
                     DAVRDRDSASRLARSVGVPVKPDDTLGQIQAALFEHSVE"
     gene            complement(1228..1503)
                     /locus_tag="AC16BC_02800"
     CDS             complement(1228..1503)
                     /locus_tag="AC16BC_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240575.1"
                     /translation="MNRMIVMLAALALFASPAMAGSMKAYQVTGPVLDLTDDSITVQK
                     GNEKWQIARDASAKITGDLKVGSKVTVEYRMTATSIEVKAGGKGPKK"
     gene            1878..2108
                     /locus_tag="AC16BC_02805"
     CDS             1878..2108
                     /locus_tag="AC16BC_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240576.1"
                     /translation="MKTMLAAVFAVMIGATFVGAAFAEDVKSEAPASAPAAPPAAGGQ
                     MKKAEVKTEKKKAKKKKAPKDAAEKKDATEKK"
     gene            complement(2186..3299)
                     /gene="prfB"
                     /locus_tag="AC16BC_02810"
     CDS             complement(join(2186..3229,3231..3299))
                     /gene="prfB"
                     /locus_tag="AC16BC_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107563908.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR3240577.1"
                     /translation="MLEDYQSRFESLRQRTIALRGYLDPARLTDRLDELERLTAEPDF
                     WNDQRKARSLTQEKARLEREMGQWRAVESMVDDVTAAIELSKGGDDSLLAELSTQLDS
                     LERTISLLEQEKLLSGEKDPNSAIVTIHPGAGGTESQDWAQMLMRMYTRWAERAGYKV
                     QVLDLQPGDEAGIKSVTFTVDGEYAYGRLRAEAGVHRLVRISPFDANKRRHTSFASVF
                     VYPEIEDDIDIVIDEKDLRIDTYRASSAGGQHVNKTSSAIRITHIPTGVVTQCQNERS
                     QHQNKEVAMKVLRAELYALEQQKKEEEMAKLTGEKKDIAWGSQIRSYVFQPYQMVKDH
                     RTGKEVGQVSAVMDGEIDPFIEAYLMQRAAGAAAKK"
BASE COUNT          554 a         1041 c         1139 g          667 t
ORIGIN      
        1 tcctcgaccg aatgttcgaa caacgcggcc tggatctggc ccaacgtgtc gtcgggtttg
       61 accggaacgc cgaccgagcg cgcgaggcgg gaggccgagt cgcgatccct gaccgcgtcg
      121 cgatccacgc cgtggcgctc cgcgatcgcg tccagcatgc cgatgcgttt ccacggcggg
      181 cgcagatcga ttgccttgcc ttggtaggtc agctccagcg tgccgcagac gtccatggcg
      241 atggacgtga tcaattcctc ggtcatgggc agcaggtcgg tcacgtccgc gtacgcgaga
      301 tagaactcca gcatcgtgaa ttcggggttg tggatcgtgg agatgccttc gttgcggaaa
      361 ttgcggttga tctcgtagac ccgctcgaac ccgcccacga tcaggcgttt gaggtacaac
      421 tcgggcgcga tgcgcagata caggtccgtg ctcagcgcgt tgtggtgcgt gatgaacggc
      481 cgcgccgcgg cgccgccggc catgccctgc atcatcgggg tttccacctc gagaaagccg
      541 cggccgttga agaacttgcg gatgccgtcg atgatgcggc tgcggcggac gaacgtgtct
      601 ttgacctcgg gattggcgat caggtccaga tagcgctggc ggtagcggat ctccacgtcg
      661 gtgaggccgt gccacttttc cgggagcggg cgcagggcct tggacaggtg cacgatccgt
      721 ttgccctcca cggtcaattc gtcggtcttg gtccggaaca cgcggccgcg cacgccgatg
      781 aaatcgccca gatcgagttg ctcgacgacg gcgaagtcgt ccggggagag cgcgtcgcgt
      841 ttgaggtaca cctggatctt gccgctcgca tcttgcaaat gcgcgaacgc ggccttgccg
      901 aaccggcgca aacccaccac gcgtccggcc acacacgcct ccggcacctc ggagtcgagc
      961 cgctcgcgcg gggtggcgcc gaatcgttgg tggacgccgg ccgcggtgtc gtccggcacg
     1021 aaccggcggc cgaacggggc gatgcccagg gacttgagcg tgtcgagttt ggcgagacgc
     1081 gagcgggttt ggtcgttcag gtcatccatc tggtggggca ctataggcca tcccacggcc
     1141 gattatcaag cggcgcgcgg actgggcgag cacaacaaaa tcacggcctg tcgatgctcc
     1201 gccgggccgg cattccgcaa cgggttgtta ctttttcggt ccctttccgc cggctttgac
     1261 ctcgatcgac gtggcggtca ttcggtactc caccgtgacc ttggaaccga ccttgaggtc
     1321 gccggtgatc ttggccgacg cgtcgcgggc gatctgccat ttctcgttgc ccttctgcac
     1381 tgtgatcgag tcgtccgtga gatcgagcac cggtccggtg acctggtacg ccttcatgga
     1441 gccggccatt gccggcgacg cgaacaatgc gagtgcggcc aacatcacga tcattcgatt
     1501 catggtttct cctcccgtgt gagttgagag cccaccgacc tgtcggtgtt tgcgatcgcg
     1561 aacccgcaag aagcttgccg ctccgtcagg gggcgttatc acacgcggag gcgaggaatc
     1621 atgcgcaggg aaccccgggc cggcgaactt ggtgcgacgt atgacgcaat tagggtgtcg
     1681 attgcctcaa atccggtggt cggatgtggt tgtgagcgcc gccacgggat atcgatcgga
     1741 acgtccggcg cagaatccgc atgccggccg gaacgcgggg ttcggcggat ggtggcatgg
     1801 gagatgcttt cttgtccggc ggttcgttgt gcatcaggga tgacacggga atcaaccgga
     1861 aggaggtgat cgagacgatg aagacgatgc ttgcggcggt gttcgcggtc atgatcggcg
     1921 cgacctttgt cggcgccgcg ttcgcggagg acgtcaagag cgaggcgcca gcttcggctc
     1981 cagcggcgcc tcccgcagcc ggcggtcaga tgaagaaggc cgaggtgaag accgagaaga
     2041 agaaggcgaa gaaaaagaaa gcgccgaagg acgcggctga gaaaaaagac gcgaccgaga
     2101 agaagtaatc gcgagaagcc cgcgtctgtc gaactgggac gccgacggcg gccggaacgt
     2161 gtcgctgtcg gcgtccgtgt cttgactact tcttcgccgc cgccccagcg gcccgctgca
     2221 tcagatacgc ctcgatgaac gggtcgatct cgccgtccat gaccgcggaa acctggccga
     2281 cctccttgcc ggtgcggtgg tccttgacca tctggtaggg ctgaaacacg tacgagcgga
     2341 tctggctgcc ccaggcgatg tcctttttct cgccggtgag tttggccatc tcctcctctt
     2401 ttttctgctg ctcgagcgcg tacaattcgg cgcggagcac cttcatggcg acttccttgt
     2461 tctggtgctg cgaccgctcg ttctggcact gggtgacgac gcccgtgggg atgtgcgtga
     2521 tacggatcgc ggacgaggtc ttgttgacgt gttgtccgcc cgcgctggac gcgcggtagg
     2581 tgtcgatgcg caggtccttc tcgtcgatca cgatgtcgat gtcgtcctcg atctccgggt
     2641 acacgaacac cgaggcgaag ctggtgtggc ggcgtttgtt cgcgtcgaac ggggagatgc
     2701 gcaccagtcg gtgcaccccg gcttcggccc gcaggcgccc gtacgcgtac tctccgtcga
     2761 ccgtgaacgt cacgctcttg atcccggcct cgtcgcccgg ctgcagatcg agcacctgca
     2821 ccttgtagcc cgcgcgttcg gcccagcgcg tgtacatgcg catcagcatc tgggcccagt
     2881 cctgcgattc ggtgccgccc gcgcccgggt ggatcgtcac gatcgcgctg ttggggtcct
     2941 tctcgccgga caggagcttt tcctgttcca gcagtgaaat ggtgcgctcc aggctgtcga
     3001 gttgggtgga gagttccgcg agcagggaat catcgccgcc cttggagagc tcgatggccg
     3061 cggtcacgtc gtcgaccatc gattcgacgg cgcgccactg ccccatctcg cgctccaggc
     3121 gggccttttc ctgcgtgagg ctcctggcct ttcgctggtc gttccagaaa tccggttcgg
     3181 cggtgaggcg ctcgagttcg tcgaggcggt cggtcaggcg agccgggtca aagatacccc
     3241 cgcagggcga tggtgcgctg gcggagcgat tcgaatcggc tttggtaatc ttcgagaatc
     3301 acggcatccc ttccttagga gcctgtccac gcaaggccat ctgcggcgtt gtcggtcggc
     3361 ctcgcgtgct cacgtacgac ccagtacgct ccgcgcgcaa g
//
LOCUS       DBNGTT010000123         2604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_24957, whole genome shotgun sequence.
ACCESSION   DBNGTT010000123 DBNGTT010000000
VERSION     DBNGTT010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_24957"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /gene="ydiK"
                     /locus_tag="AC16BC_02815"
     CDS             complement(<1..883)
                     /gene="ydiK"
                     /locus_tag="AC16BC_02815"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HIR3240578.1"
                     /translation="MRNADRDLPRDILAVLFIGTLMGASLWILRPFLVAIIWATTIVV
                     ATWPAMLAVQARLWGKRALAVMVMTLVLLAVLIVPSVALLGTIVANVEPIVGWVQSIA
                     IDTLPPAPAWVRDLPLIGELAAGTWEQIAAKGVQELLVRAAPYAGEVTTWFVGQVGHL
                     GLVVVQSVLTIVIAVILYAYGEPVAGGVRRFASRLAGAPGEAAVGLSARAIRGVALGV
                     VVTAMVQAGLGGLGLAVAGVPFAAVLTTVMFLFAVAQIGAVPVLVPPVIWLYWSGDAT
                     WGTFLLIVTIIVGTLDNV"
     gene            1207..1977
                     /locus_tag="AC16BC_02820"
     CDS             1207..1977
                     /locus_tag="AC16BC_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240579.1"
                     /translation="MSNEDIHSQAAAPSPAFRRGEIFWHRGQLEAARQCFEEALTVER
                     RLGEPARVMPVLLSLGSVHGALGNRKAARACLQEILGLQREAPDAQLAGQALVNLGNL
                     AREEGERERARAYYLEALDLLSPLGDTRSLGILHSNVGLLELDAGRLDDAISSLKTAI
                     ELHKRSGFEEGLADTWDQLGRAFLRSGRPDKAETCFNFSSSHFISLGNPAGEAEALRG
                     LTAVHEARGDAELVRRCRERIAEIQIRYGLAPGDRSSR"
     gene            complement(1991..>2604)
                     /locus_tag="AC16BC_02825"
     CDS             complement(1991..>2604)
                     /locus_tag="AC16BC_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3240580.1"
                     /translation="LDGQSREFREAGASMYVAFALALAFLYLVLAAQFESFVDPFIIM
                     LSVPLAVAGALLSLELTGNTLNVYSQIGMVMLVGLIAKNGILIVEFANQLQERGRSVR
                     DAVVEAASLRLRPILMTTAAMILGSLPLALAHGAGAESRQQIGWVIVGGLLLGTLLTL
                     FVIPTAYTLLARRRVGPDESAGQDIEVRALAGNGEGAGVGIEV"
BASE COUNT          506 a          886 c          825 g          387 t
ORIGIN      
        1 ggacgttgtc gagcgtgccg acgatgatgg tgacgatgag cagaaaggtt ccccacgtcg
       61 cgtccccgct ccaatacagc cagatgacgg gcggcacgag caccgggacc gctccgattt
      121 gcgccacggc gaacagaaac atgaccgtgg tcaggaccgc cgcgaacggc acgccggcca
      181 ccgcgaggcc gagtccaccc agtcccgcct gcaccatcgc ggtgaccacg accccgagcg
      241 ccaccccgcg aatcgcgcgc gcggacaggc ctaccgccgc ctcgccgggc gcgccggcga
      301 ggcggctcgc gaagcgtcga acacccccgg ccacgggctc accgtacgcg tacaggatca
      361 ccgcgatcac aatggtcaac acggactgaa cgaccaccaa gcccagatgc ccgacttggc
      421 cgacgaacca tgtcgtcacc tccccggcgt acggcgcggc ccgtaccagg agttcctgga
      481 cccccttggc cgcgatctgc tcccacgttc ccgccgccag ttcgccgatc agcggaaggt
      541 cccgcaccca ggccggcgcc ggcggcaggg tgtcgatcgc gatcgactgg acccagccca
      601 cgatcggctc gacgttcgcc acgatcgtgc cgagcagggc cacggagggc acgatcagca
      661 ccgccaacag cacgagcgtc attaccatca ccgcgagcgc ccgcttgccc cagagacggg
      721 cctgcaccgc gagcatggcg ggccacgtcg ccaccacaat ggtcgtggcc cagatgatcg
      781 ccaccagaaa cggccgcaga atccacaacg acgcgcccat cagcgtgccg atgaagagta
      841 cggcgaggat gtcgcgggga agatcgcggt cggcgttacg cacggtccgt catcacatgg
      901 cgcgtctgtt ggagtgatgg gcgtgactcc cgatcatgtc tgcaccatgt gggggatgat
      961 tcgcccgcaa tccgcatcga gtcgctgaac ggcgcgtcac tgcgcacggg tgtcctcgct
     1021 cctgtcggtt gcgacatcct cgggtctccg aacgacgaag tctctgatgc gacggaaccc
     1081 accctagcat aggggtgaaa aatgattcaa gcgcgacgcg acgcaccacc ctaggctccg
     1141 cctgcgtgga gcgtgcgggt ggaacgcgga ttgacaggcg tctagttcgg gtgttacagg
     1201 caaaccatgt cgaacgagga catccattcg caggcggccg cgccatctcc cgcgtttcgc
     1261 cgcggcgaga tcttttggca ccgcggtcag ctcgaagcgg cgcggcagtg tttcgaggag
     1321 gcgctgaccg ttgaacgccg actcggcgag cccgcgcggg tgatgccggt gttattgagt
     1381 ctcgggagcg tacacggggc gctcggcaac cgcaaagccg cgcgcgcctg cctccaagaa
     1441 atactgggac tgcagcgcga ggctccagac gcgcaactcg cggggcaggc gctggtcaac
     1501 ctgggcaacc tagcgcgcga ggagggcgag cgcgaacggg cgcgggcgta ctatttggaa
     1561 gcgctggatc tcctctcccc gctcggcgat acccggtcgc tgggcattct gcacagcaac
     1621 gtggggttgc tggaacttga cgcggggcgg cttgatgacg ccatttcctc gctcaagacc
     1681 gcaatcgaac ttcataaacg ttcgggattc gaggaaggcc tggccgacac ctgggaccaa
     1741 ctgggacggg cgttccttag atcgggcagg cctgataagg cagagacgtg ttttaacttc
     1801 tcgtccagcc atttcatcag cttgggcaac ccggcgggcg aggccgaagc cctgcgggga
     1861 ttgactgcgg tccatgaagc gcggggtgac gcggaactgg tcaggcggtg ccgagagcgg
     1921 atcgcggaaa ttcagattcg atacggactc gcgcccgggg accgctcgtc ccgctgactc
     1981 gatcgaagcg tcagacctcg atcccgaccc ccgcgccttc accattgccc gccaacgcgc
     2041 gcacttcgat atcctggcca gccgactcgt cgggtccgac gcgtcgacga gccaacaacg
     2101 tgtacgcggt cggaatcacg aacagggtca acaacgtccc cagcagcagc ccgcccacga
     2161 tgacccagcc gatctgctgg cggctctccg cgcccgcgcc gtgtgccaat gccagcggga
     2221 gcgagcccag gatcatcgcc gccgtggtca tcagaatcgg gcgcaaccgc agcgatgcgg
     2281 cttccaccac cgcgtcccgc accgagcgcc cccgttcctg gagttgattg gcgaactcca
     2341 cgatcaagat cccgttcttg gcgatcaaac ccaccagcat gaccattccg atctggctgt
     2401 acacgttcag ggtgttgccg gtcaattcga gcgacagcag cgcgccggca acggccaagg
     2461 ggacggagag catgatgatg aacgggtcga caaaactctc aaactgcgcg gcgagcacca
     2521 gatacaaaaa cgccagcgcc aaggcgaatg cgacgtacat gctggcaccc gcctccctga
     2581 attcgcgtga ctggccgtcg agcg
//
LOCUS       DBNGTT010000124         4190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_664078, whole genome shotgun sequence.
ACCESSION   DBNGTT010000124 DBNGTT010000000
VERSION     DBNGTT010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_664078"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /gene="rpsU"
                     /locus_tag="AC16BC_02830"
     CDS             complement(<1..150)
                     /gene="rpsU"
                     /locus_tag="AC16BC_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3240581.1"
                     /translation="MKIVVSDNQVDKALRVLKKKLIKEGLLKEMKKREYYEKPSVKRK
                     RKEKES"
     gene            complement(247..1434)
                     /locus_tag="AC16BC_02835"
     CDS             complement(247..1434)
                     /locus_tag="AC16BC_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3240582.1"
                     /translation="MSEPRRALFSLPRTVVMLGVVSLLTDASSEMIYPLLPIFLATTL
                     GAGATVIGLIEGVAESTASLVKLGSGWWSDRLGRRKAIVVAGYTLSACARPLVALAGA
                     GWHVLVIRFADRVGKGLRSAPRDALLAASTPAQSWGKAFGFHRAMDHTGAIIGPLIAT
                     ALLAWGLTDLRVLFALAAIPGILAVVVLVSAVREHAPVDRAAAAPPRLSLAPFSPRFR
                     AVLGVVALFTLGNSSDAFLLLKANAAGIGPAWIPLLWILLHITKAASSTPAGTLSDRI
                     GRPRLILAGWLVYAAVYAGFGLATAAWQIWALFAIYGLFFGLTEGVEKAYVADLAPAE
                     HRGTAYGLYHVAVGLAALPASVMMGVVWEHVGPGAAFGLGAGLALLASLLFLAALRVP
                     SRT"
     gene            2310..2963
                     /locus_tag="AC16BC_02840"
     CDS             2310..2963
                     /locus_tag="AC16BC_02840"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3240583.1"
                     /translation="MAKNGVLYGVMAVLFLLAGPVSAQMGKDGKGQAGMMGEGMMGGG
                     MGMEMMGAHGGGMGMGMGGMDDMMKMMRAASKLNLTGEQQKKLQLSTLQHQKEAIPML
                     GQIRMAGVEIQELLLSDPVNLDKVKSKVKEKYDAAAKLEMSHLTLTQDVKKMLTPEQR
                     RQMESMMMRMGPMMGGMPMMDSMMGAPSGPATAPQAPQDAPAKPSDDASDSKDPHGH"
     gene            2976..3260
                     /locus_tag="AC16BC_02845"
     CDS             2976..3260
                     /locus_tag="AC16BC_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HIR3240584.1"
                     /translation="MTTDHPWRTGATLALTMTITYTVCALAYALAPERGVEFLNALFH
                     GLDFHKLETAAPFTFLMFMYPLVVFVVWGFAVGTLFSWLHQALHRVRGPG"
     gene            3297..>4190
                     /locus_tag="AC16BC_02850"
     CDS             3297..>4190
                     /locus_tag="AC16BC_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR3240585.1"
                     /translation="MAIDPVCGMTVDEDSAEGKTVFDGRTYYFCSAHCLRAFEANPRP
                     FLSVRPGDEAHTVRVGHAMPAGPAATKTTKPSKDMAKDPICGMVVDKATALKTERGGR
                     AYYFCSAGCQRTFESPEQELKSMRTRVTIALTGVLALAVLRAAAFLALATGATIVTWA
                     PIPQLPWFTWGVWLFLLVTPVQFIGGWSFYKGSWSAIRTRTINMDFLIALGTTVAYVY
                     SVAVIFVPWVLPVKVEERDVYFEVSAVIIAFVLLGKYMEEMIKKRSSAAVRKLLDLKP
                     ATARVIREGADMEVPAETVMVG"
BASE COUNT          803 a         1234 c         1388 g          765 t
ORIGIN      
        1 cgattccttt tccttgcgtt tgcgtttcac gctcggtttt tcgtagtact cgcgcttttt
       61 catctccttg aggagcccct ccttgatgag cttctttttg aggacccgga gggccttatc
      121 gacctgatta tcgctgacga cgattttcat tgcctggtgt cacaacctcc gcggtcggct
      181 cctcaacaac gacggcgcag gataccggaa cattctgcgg gatgtcaagt ggtttttcgc
      241 ctttttttat gtccgggacg gaactcgaag cgcggcaagg aacaacaacg aggccaacag
      301 cgccaagccg gcgccgagcc cgaacgcagc gcccggtccc acgtgctccc agaccacacc
      361 catcatcacg ctggccggga gggcggcgag ccccaccgcg acgtgataca ggccgtaggc
      421 ggtgccgcgg tgttcggcgg gcgccaggtc cgccacgtag gccttctcaa cgccctcggt
      481 caagccgaaa aagagcccgt agatcgcaaa cagcgcccag atctgccagg ccgcggtggc
      541 cagtccaaag ccggcgtaga ccgcggcgta gaccagccac cccgcgagga tgagacgcgg
      601 ccggccgatt cgatccgaca gggtgccggc gggggtggaa ctcgcggctt tggtgatgtg
      661 caggaggatc cacaacaagg ggatccacgc cggcccgatg cccgcggcgt tggctttgag
      721 cagcaaaaac gcatcgcttg agttgccgag ggtgaagagc gcgaccacgc ccagcaccgc
      781 ccgaaaacgc ggggagaacg gggccaggct gagccgaggg ggcgcggcgg ccgcgcgatc
      841 aacgggtgcg tgttcgcgca cggcggacac caacacgacc accgcgagaa tcccggggat
      901 cgcggcgagc gcaaagagca cccgcagatc ggtcaacccc caggccagga gcgcggtagc
      961 gatgagcggg ccgatgatgg cgccggtgtg atccatggcc cggtgaaacc cgaaagcctt
     1021 gccccaggac tgggcgggcg tggacgcggc caacaacgcg tcgcgcgggg ctgatcgcaa
     1081 ccccttgccc acgcggtcgg cgaatcgaat caccagcacg tgccaccccg cgccagccag
     1141 cgccaccagc ggtcgcgcgc aggccgacag cgtgtacccc gcgaccacaa tagccttgcg
     1201 gcggcccagc cggtccgacc accaccccga cccgagcttg accaagctgg ccgtgctctc
     1261 cgccacaccc tcgatcagcc cgataaccgt cgcgcccgcg ccgagcgtgg tggccaggaa
     1321 aatgggcagg aggggataaa tcatttcgct gctcgcgtcg gtcaacaagc tgaccactcc
     1381 gagcatgacc acggtacggg gcaaggagaa gagggcgcgg cggggctcgg acatgggagg
     1441 cggagtatgg cagcgcggtg cgggaggagt caaacgctgt tggattttgc ataattccgc
     1501 gcggcagcag gtgccctggt gagaaaccat ggtttggtag ctcgttccgg aagccgaccg
     1561 tggtgctggt tccgaaaata cttgccggtg tcgggacctg gaggttgtcg cccaattggg
     1621 gtgagccgaa ctcgctccta atctcgcggg aggggcctcc tcatgggtat ggcaatgttg
     1681 tagcctctgt cctagactgg atctgaaaaa gggaaagtag tatagtcacg acgcctatca
     1741 cgagtctgca gaaggggggg gatgtcgata gtcgctcgtc aagagtttcg caccccgcga
     1801 aatgccgcgt tgttcttgat cgcgtttttt gtcgtctgtc tgctctgcca ggctttccct
     1861 gaaaccgctg ccgcgcatca ttccggctcg tcggaatctg accatacctg tgccaccgtc
     1921 gcagaggttt cgcccgtctc tccggtcggt cttttggtcg gatcattcgc cactgccggt
     1981 tcgagctgtt tcccggcgga ggtgttccca tccggtcctg catcgtgggg cgggggagac
     2041 ctgagcggcc cgatgtgtcc gcagccggac gcaatgtccc caccgatctt tcccgccatc
     2101 tattgtctca actgtacgta ccggctttaa ctcgttgttg aactgatttt ccggtcggtc
     2161 ctgctccgac cagtgtgacc acgtggtcgc cggagtcgac cgactccggt cgcggctgtg
     2221 acttgtcatg cggcgggatc tgcgcgaatc atttgatggg gatgtcacgg acaccaaacc
     2281 gggggtattc agagaaagga gaatcggcga tggcgaaaaa cggtgtgctc tatggtgtta
     2341 tggcggtgtt gttcttgttg gcggggccgg tctcggccca gatgggaaag gacggtaagg
     2401 gacaggccgg tatgatgggc gagggcatga tgggcggtgg aatgggcatg gagatgatgg
     2461 gcgcccatgg tggaggcatg ggtatgggca tgggcgggat ggacgacatg atgaagatga
     2521 tgcgggcggc ctcaaagctg aacctgaccg gcgagcaaca gaagaagctc caactctcca
     2581 ccctccaaca tcagaaagag gcgattccga tgttggggca gatccgcatg gcgggcgtcg
     2641 agatccagga actgctcctc tcggatccgg tgaatttgga caaggtcaaa tccaaagtga
     2701 aagagaagta tgacgccgcc gcgaagcttg agatgagcca tcttacgctg acgcaggacg
     2761 tcaagaagat gctgacgccg gaacagcggc ggcaaatgga atccatgatg atgcggatgg
     2821 gaccgatgat gggcggcatg ccgatgatgg attccatgat gggggcgccg tcggggcccg
     2881 ccaccgcgcc gcaagcgccc caggacgcgc ccgccaagcc ttccgacgac gcgtcggact
     2941 ccaaagaccc gcatggccat tgaggaaggg atcagatgac aaccgatcac ccgtggagaa
     3001 caggggcgac attggccctg acaatgacga tcacgtatac ggtgtgcgcg ttggcctacg
     3061 cgctggcgcc cgagcgcgga gtcgagttct tgaacgcgtt gtttcacgga ctcgactttc
     3121 acaaactcga aaccgcggcg ccgttcacgt ttctgatgtt catgtacccg ttggtggtgt
     3181 tcgtggtctg ggggttcgcc gttggaacgc tcttttcgtg gttgcatcag gcgcttcatc
     3241 gtgttcgcgg gcccggctga gcgttgaacc ggacaaagag gatccgagga ggcgcgatgg
     3301 cgattgatcc ggtgtgcggg atgacggtgg acgaggattc cgccgagggc aagacggtct
     3361 tcgacgggcg gacgtactac ttctgctccg cccactgttt gcgggcgttt gaagccaacc
     3421 cgcggccatt tttaagcgtg cggcccgggg atgaagcaca cacggtaagg gtgggccacg
     3481 caatgccggc gggtcccgct gcgaccaaaa caacgaagcc gtccaaggac atggccaagg
     3541 acccgatctg cggcatggtg gtggacaaag ccacggcgct caagacggag cgaggcggac
     3601 gggcctacta cttttgcagc gcggggtgtc agcgcacgtt cgagtcgccc gaacaggaac
     3661 tcaaatccat gcgcacccgc gtcacgatcg cgctcaccgg cgtgctcgcc ttggctgtgc
     3721 tccgtgcggc cgcgttcctg gccttggcca ccggagcgac catcgtcacg tgggcgccga
     3781 tcccgcaatt gccctggttc acgtggggcg tgtggctgtt cctgttggtc acgccggtgc
     3841 agttcatcgg cgggtggagt ttctacaaag ggtcctggag tgcgatccgc acccgcacga
     3901 tcaacatgga cttcctgatc gccctgggaa caaccgtggc ctacgtgtac agcgtggccg
     3961 tgatcttcgt tccctgggtg ctgccggtga aggtcgaaga gcgggacgtg tacttcgaag
     4021 tctcggccgt gatcatcgcg tttgtactgc tcggaaaata catggaggag atgatcaaga
     4081 agcgctcatc ggccgcggta cgcaagctgc tcgacctcaa gcccgcgacc gcacgagtga
     4141 tccgcgaagg cgctgacatg gaggtgccgg ccgagaccgt gatggtcggc
//
LOCUS       DBNGTT010000125         1637 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_408511, whole genome shotgun sequence.
ACCESSION   DBNGTT010000125 DBNGTT010000000
VERSION     DBNGTT010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_408511"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..>1637
                     /locus_tag="AC16BC_02855"
     CDS             70..>1637
                     /locus_tag="AC16BC_02855"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR3240586.1"
                     /translation="MTKYLFITGGVVSSLGKGLASASIGNLLESRGIKVAFLKLDPYI
                     NVDPGTMNPYQHGEVFVTEDGTETDLDLGHYERFTTVTTGRQNNFTTGRIYYNVIQKE
                     RRGDYLGGTVQVVPHVTDEIKRSITALGDSTDVVIVEIGGTVGDIESLPFLEAIRQFP
                     FDVGRENVLYVHLTLVPYVAASGELKTKPTQHSVNKLREIGIQPHILLCRSERAVPDD
                     LKAKIALFCNVDRDAVIAARDVETIYDVPLVFHQQGLDELIARHLHLPSRPANLGDWE
                     RLVERIKHPHGSVNIALIGKYLGLKDSYKSLSEALHHGGFAHTTRVDISWIDAENLLA
                     DGPEPHLTDMDGILVAPGFGNRGIEGKIEAIRYARERHAPFLGICLGMQCAVIEYGRH
                     VLGLSAANSTEFQATPDPVIDLMRDQHAVEGKGGTMRLGSYPCRVAKGSRAFEAYGRE
                     EVSERHRHRYEFNNGYRERFEQGGLALSGINPQRDLVEIVELPSHPWFVGVQFHPEFR
                     SRPLDPHPLFTAFVG"
BASE COUNT          333 a          502 c          515 g          287 t
ORIGIN      
        1 caacacccct gaggatcttg aacgcgccag ggcggaagct gccgcggtaa cgacggcggg
       61 gaggatgtca tgacgaaata cttgttcatc acgggcgggg tggtgtcgtc gctgggcaaa
      121 ggcctggcgt cggcctcgat cggcaatctc ctcgagagcc gcggcatcaa ggtggcgttt
      181 ctcaaactcg atccctatat caatgtcgat cccggcacca tgaatcccta ccaacacgga
      241 gaagtcttcg tcaccgagga cgggaccgag accgatctgg atctgggaca ctacgagcgg
      301 tttacgacgg tgacgaccgg ccggcaaaac aatttcacca ccggacgcat ctattacaac
      361 gtgattcaga aggaacgccg gggcgattac ctcggcggaa ccgtgcaggt ggtgccccac
      421 gtcaccgatg aaatcaaacg ctcgatcacg gcgctggggg attcgaccga cgtggtgatc
      481 gtcgagatcg gcggaaccgt cggcgatatc gagagtctgc cgtttctgga agcgatccgc
      541 cagttcccgt tcgacgtggg gcgggagaac gtgctctacg tccatctcac gctcgtgccg
      601 tacgtggcgg catccggcga actcaagacc aaacccacgc aacacagcgt gaacaaactg
      661 cgcgagatcg gtattcagcc gcacatcctg ctctgccggt ccgagcgggc ggtgccggac
      721 gacttgaaag ccaagatcgc gttgttctgc aacgtggacc gcgacgcggt aatcgcggcg
      781 cgcgatgtgg aaaccatcta cgacgtgccg ctggtgtttc accaacaggg tctggacgag
      841 ttgatcgccc gtcatctcca cctcccgtcg cgccccgcga acctggggga ttgggagcgg
      901 ttggtcgaac ggatcaaaca tccgcacggc tcggtgaaca tcgcgctcat cggcaaatac
      961 ctaggactca aggattcgta caagagcctg tcggaggccc tgcaccacgg cggattcgcc
     1021 cacacgacgc gcgtggacat ctcctggatc gacgccgaaa acctcttggc cgacgggccg
     1081 gagccgcatt tgaccgacat ggacgggatc ctggtcgccc cgggattcgg caaccgcggc
     1141 atcgaaggaa aaatcgaagc cattcggtac gcgcgcgaac gacacgcgcc gtttctcgga
     1201 atctgcctcg gcatgcaatg cgcggtcatc gaatacggac gtcacgtgct cggactgtcc
     1261 gcggccaaca gcaccgagtt tcaggccacg cccgatccgg tgatcgacct gatgcgcgac
     1321 cagcacgcgg tggaaggcaa gggcgggacc atgcgtctgg gctcgtaccc gtgccgggtc
     1381 gcaaagggca gccgcgcgtt cgaggcatac ggtcgcgaag aagtcagcga gcggcatcgc
     1441 catcgctacg agttcaacaa cggctaccgg gagcggttcg agcagggcgg gttggcgctc
     1501 agcggcatca acccccaacg cgatctggtt gaaatcgtcg agttgccgtc tcatccgtgg
     1561 ttcgtggggg tgcagtttca tccggagttt cgttcgcggc cgttggatcc tcacccgctg
     1621 ttcaccgcgt tcgtcgg
//
LOCUS       DBNGTT010000126         8159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2709010, whole genome shotgun sequence.
ACCESSION   DBNGTT010000126 DBNGTT010000000
VERSION     DBNGTT010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2709010"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC16BC_02860"
     CDS             <1..231
                     /locus_tag="AC16BC_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240587.1"
                     /translation="IESTWGGVLMLKLFLVLVLIGLSVIHDFILDPYGPGADRGPAAR
                     IAARARTAARVQHAIIVVSIAVLLVAAYLARM"
     gene            261..746
                     /locus_tag="AC16BC_02865"
     CDS             261..746
                     /locus_tag="AC16BC_02865"
                     /inference="COORDINATES: protein motif:HMM:NF017913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HIR3240588.1"
                     /translation="MSRVTTRQSSIELMERLETYGYVSVGVSFLVLGMAVFAYGWFDF
                     IQSVQGHVPRAVLVLLNDLLLVVILLELFHTIVNFLRTREISLEPFLHVGIIAAVRKI
                     LTVGAEMVLVETIDHERFNQYLFDVGVHGLVVLALVTGLYFYRASRRQLPASAPPRTT
                     P"
     gene            947..1234
                     /locus_tag="AC16BC_02870"
     CDS             947..1234
                     /locus_tag="AC16BC_02870"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3240589.1"
                     /translation="MTVERVVKVRVQDGERAATLQDLSLTGCFLATSAAYAVGTILSL
                     RFELEGGTPQPVATEARVVRRTPTGVGVRFVFTDPQTPAAIKRWIADRRPG"
     gene            complement(1231..2295)
                     /locus_tag="AC16BC_02875"
     CDS             complement(1231..2295)
                     /locus_tag="AC16BC_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240590.1"
                     /translation="MREHRWETRTPIGFDDVLALCRRLEGLGLPVRPADRVGACYFEE
                     CWVDRLEDIGRLDAWPIDEVTLIEVDDTWTGDFFVLAGRHHELYRERASMEAYLSLSH
                     PWRIPLDGDLTAHHRDAMFWIGFRDTHGFIRVRVVPREIIAPREGEQDARRDEWVRER
                     AEAFTAALEALGLPLFVDWSEGMPTVGSAEPGCRISGSWPDAFGPCQFEYVVPDRYEL
                     LVPAARLVERLGAPRPVLRTFLSGWPPADLEAFHRLQPTVRLLYRGFVHASLNDLPAI
                     ASAIAPRGRALATVCEFPTSGLLPDGRDAYAIIGVIGGQDGFSVEIRLSRMPLSVGAT
                     GGWIEDLVKAAVDYSPLGLY"
     gene            complement(2420..3853)
                     /locus_tag="AC16BC_02880"
     CDS             complement(2420..3853)
                     /locus_tag="AC16BC_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3240591.1"
                     /translation="MKGFSVEARVGAVVALALGALLWASFQLKDFAFRSVEGFRVSTL
                     FESAAGLDKNAPVKMAGVPIGEVEAMALEDSRAKVSMRIRPGYRIPNGSRAVVKAAGL
                     LGDKYVEILPGREPGFVGDGAELPQAEVTADLDTLINRFGAIADDVKAVTASLRNALG
                     TGEGERSLKEIVSHFRNVAENLDHIVAENREGVKATVAHAEEFMRALREDGPKLVATL
                     NRIADRLERGDGTLGKLLTDEAAYKKLDDALASLNTVSKNLESGKGTLGKLINDDQAY
                     EKLNTALTGLSGTLNKIDQIRLTLDARNEYQFSEGDNKGYFGLHVTPRENKSYIVEVV
                     DDPHGKVTKTTTVRTTNGVTDTIEDLKFERKIKFSVEYYRRFGNLGLRGGLMENSFGV
                     GADYFLGVPGLELSLDFWDFNSDDPLNDNVHAKATARYRFLTYLYLQAGYDNPFNRDL
                     DTAFVGGGLTFDDEDLKYLIGSGMLRR"
     gene            complement(4010..4753)
                     /locus_tag="AC16BC_02885"
     CDS             complement(4010..4753)
                     /locus_tag="AC16BC_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240592.1"
                     /translation="MIEIEDLHKSFGAKVVLAGLTLTIERGETMVVIGGSGTGKSVLL
                     KHVIGLMKPDQGRVVVDGQEVGALNETQLTELRKKFGMLFQGAALFDSLSVWENVGFW
                     LTQHAKRSPAAIREIAREKLALVGLKGVEDLMPAELSGGMKKRVGLARAIAHDPQIML
                     YDEPTTGLDPIMADVINELIIRLKHQLKMTSIAITHDMTSAYKIADRIAMLYQGRIQE
                     VGTPDEIRASRNPIVRQFITGSAEGPIAV"
     gene            complement(4755..5525)
                     /locus_tag="AC16BC_02890"
     CDS             complement(4755..5525)
                     /locus_tag="AC16BC_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR3240593.1"
                     /translation="MIRWLERLGASAITLLHEFGNLSLLLWNVVRGALVRPLDFRLHI
                     KQMEEVGTKSIPVVAITGLSTGMVLALQSYTGFQRFQAESLVGTVVALSMTRELGPVL
                     TALMVAGRAGAAMAAELGTMRVTEQVDALASMGVNPVKYLVLPRFLAGLLMLPVLTVF
                     SDIIGVAGGLGVSVFVLDANPTIYMKRTFDYLEVADVYGGLLKAIVFGGIIALVSCQR
                     GFHARGGAEGVGQATTGAVVVSCVLILISDYIMTALLY"
     gene            complement(5522..6709)
                     /gene="alr"
                     /locus_tag="AC16BC_02895"
     CDS             complement(5522..6709)
                     /gene="alr"
                     /locus_tag="AC16BC_02895"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3240594.1"
                     /translation="MTHATPALRPTVARVDLGAIRFNVGQARTLAGGRHLIAVVKADA
                     YGHGAAAVARAIEADGVRRFGVALVEEGRVLREAGVRGDIIVLGGFAPEQARDVVDLG
                     LSAAIFHPEHAAALDAAAVRAGRVSPVHVKVDTGMGRLGFPVEAAAQAVERVAQRPGL
                     RLDGLMTHFADADLADPAYAREQIARFDAVSEAVARAGIKIPIRHAANSAALIKGTRS
                     LGAAPGGRPSDGPLYDAARPGIMLYGCRPGPDVDGVALKPALSLTTTILLLKRVPPNT
                     PISYGRTFVTRRESLIAVLPIGYADGYPRALSNRGAVLIRGRKAPVAGRVCMDLTMVD
                     ATDVPGVAEGDEVVLIGSQDGASLPAEDVAETAGTIAYELLCGIGPRVPRRYLNGEGA
                     GSR"
     gene            complement(6706..7878)
                     /locus_tag="AC16BC_02900"
     CDS             complement(6706..7878)
                     /locus_tag="AC16BC_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240595.1"
                     /translation="MNRIVPAWIMRRSAGSVALAVGWLLVASGCGVLGGVHVVTLHDP
                     LSPDEHVALGVAYERDGKLDRAADEYKSALRAQPDHAQALVNLGNLAANAGAYADAEA
                     WYARAVRTGAPLAAPGANNLAWLYLTQGKRLASAESLARKALAWDPRPEYLDTLVDVL
                     LKLGKPLDASRAIDDGLNQWRDDASLLAKVRDMLADDHQEIARLAFEQGKAKAAAGRY
                     DEAERLLREAVRRDTERTADYPVLLAQVLLDSGRPEEAVRVIDEARTGAAAAAEFAAK
                     ARAFAQTAHARSQKLFQEGAVEPAESLVRGALRWDPDRAPYYLDTLAQVLIAREKPYE
                     AGKVIDEAEALAPVGDAELRARLFDRKAELFASKGLAAEAKAAAQQADAIRKAATQ"
     gene            complement(7875..>8159)
                     /locus_tag="AC16BC_02905"
     CDS             complement(7875..>8159)
                     /locus_tag="AC16BC_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240596.1"
                     /translation="AVRAEAGTLAWLRARVADDHPVIAFLDLGFGPVRQGHFVVVVGY
                     DDADERVLLYSGRDAGATMSYRRFASSWGRGGWWALAPTERRDSAVAPGS"
BASE COUNT         1390 a         2855 c         2513 g         1401 t
ORIGIN      
        1 atcgaatcca cgtggggcgg cgtgttgatg ctcaagctgt tcttggtctt ggtgttgatc
       61 gggttgtcgg tcatccacga ttttatactc gatccgtacg gtccgggggc cgaccgggga
      121 ccggccgccc ggatcgccgc gcgcgcgcgg accgcggcgc gggtgcagca cgcaatcatc
      181 gtcgtctcga tcgcggtgct gttggtcgcc gcgtatttgg caaggatgta actccccggt
      241 accggctggt ttggagcgtt atgtcgcgcg tcacgacacg gcaatcgtcg atcgaattga
      301 tggaacggct cgagacctac ggctacgtgt ccgtcggcgt cagcttcctg gtactcggga
      361 tggcggtgtt cgcgtacggc tggttcgatt tcatccagtc cgtgcagggc cacgtacccc
      421 gggcggtcct ggtgttgctc aacgacctgc tgttggtggt catcctgctc gaactgtttc
      481 acaccatcgt caacttcctc cgaacccggg aaatcagcct cgaaccgttt ttgcatgtcg
      541 gaatcatcgc cgcggtccgc aaaatcctca ccgtcggggc cgagatggtg ctcgtggaaa
      601 cgatcgacca cgagcgattc aatcagtacc tgttcgacgt cggagtccac ggcctggtcg
      661 tgctggcgtt ggtcaccggc ctgtattttt accgagcaag cagacgccaa cttccggcga
      721 gtgccccgcc ccggacaaca ccctgaggga tcacatgact ccggcccgcg ggctgcccgc
      781 gtcgagcggg gcagaacaaa caaccggata accaggcggc gtgggtcggt gtcgccggaa
      841 cggcatgcgt gacgccgcga aacagcctcc tatttggtaa ggataagcaa tgggagagca
      901 aggaccccga tctgacaccc cctccgacgc tcggtggggc gaccgcttga cggtcgaacg
      961 cgtcgtcaaa gttcgcgttc aggacggcga gcgagcggcg acattgcagg acctcagttt
     1021 gaccgggtgt ttcctggcca cgtcggccgc ctacgcggtg gggacgattc tctcccttcg
     1081 gttcgaattg gaaggcggaa cgccccaacc cgtcgccact gaggcgcggg tggtcaggcg
     1141 cacgccgacc ggcgtcgggg tgcgattcgt gtttaccgat ccgcagactc cggccgcgat
     1201 caaacgctgg atcgcggacc gccggccggg ctaatacaac ccgagcggcg aatagtcgac
     1261 cgccgctttg acgaggtcct cgatccatcc gccggttgcg ccgaccgaga gcggcatgcg
     1321 gctcagtcgg atctcgacgg aaaacccgtc ttgcccgccg atcaccccga tgatcgcgta
     1381 cgcatcccgg ccgtccggga gcaacccgct ggtgggaaat tcgcagaccg tcgcgagcgc
     1441 gcgtccgcgc ggggcgattg ccgaggcgat cgcgggcaga tcgttcaggg acgcgtggac
     1501 gaacccgcgg tagaggagtc tcacggtcgg ctgcaatcgg tggaacgcct ccaagtcggc
     1561 cgggggccat ccggacagaa acgttcgcaa caccggtcgt ggcgccccaa ggcgttcgac
     1621 caacctggcg gcgggcacga ggagttcata ccgatccggc accacatatt caaactggca
     1681 cgggccgaac gcgtcgggcc acgatcccga gatgcggcag ccgggctcgg ccgaacccac
     1741 cgtcggcatc ccttcggacc aatccacgaa cagcggcaac cccagcgcct cgagcgccgc
     1801 ggtaaacgcc tcggctcgct cccgcaccca ctcatcgcgc cgcgcatcct gttcgccttc
     1861 ccgaggcgca ataatttccc tcggcacgac gcgaacccgg atgaacccgt gggtgtcgcg
     1921 aaacccgatc cagaacatcg cgtcccgatg gtgggcggtg aggtccccgt ccagcggaat
     1981 ccgccacgga tgactgagcg acagatacgc ttccatggac gcgcgctcgc gatacaactc
     2041 gtggtggcga ccggccagga caaaaaagtc gcccgtccat gtgtcgtcga cctcgatgag
     2101 cgtcacttcg tcgatcggcc aggcatccag gcgcccgatg tcctccagcc ggtcgaccca
     2161 acactcttcg aagtaacacg cgcccacgcg gtcggccggc cgcaccggca aacccaatcc
     2221 ctccaaccgc cggcacaatg cgagcacgtc atcgaagccg atcggcgtgc gggtttccca
     2281 acggtgttca cgcatgggga ggatcgaggt cagaccgctt cacgccgcgg cggcatcgcg
     2341 tgatttgtca tgcaccctct cggcgacaac cctgatatcg agagagccga cgcgcggaca
     2401 cgcgggcggg gcaccggttc tatcgccgaa gcataccgct gccgatcagg tacttcagat
     2461 cctcgtcgtc gaacgtcaac ccgccgccca cgaacgcggt atccagatct cgattgaagg
     2521 ggttgtcgta accggcttgg aggtagaggt acgtgaggaa ccggtaccgt gccgtggcct
     2581 tggcgtgcac gttgtcgttg agcggatcgt cgctgttgaa gtcccagaag tccaggctca
     2641 gttcaagacc gggaacgccg aggaagtagt cggcgcccac gccgaagctg ttttccatca
     2701 accctccgcg tagcccgagg ttgccgaacc ggcgatagta ttccacggaa aatttgatct
     2761 tccgctcgaa tttgaggtcc tcgatggtat ccgtcacccc gttcgtcgtg cggaccgtcg
     2821 tggtcttcgt gactttgccg tgcgggtcgt ccaccacttc cacgatgtaa ctcttgttct
     2881 cgcgcggggt gacgtgcagc ccgaagtacc ctttattgtc cccctcgctg aattggtact
     2941 cgttgcgagc gtccagcgtc agccggatct ggtcgatctt gttgagcgtg ccgctcaagc
     3001 cggtgagcgc ggtgttgagc ttctcgtagg cttgatcgtc gttgatcaat ttgcccagcg
     3061 tgcccttccc gctctccaag ttcttggaca cggtgttgag gctggccaag gcgtcgtcca
     3121 gcttcttgta cgcggcctcg tcggtcaaca gcttgcccag cgtgccgtcc ccgcgctcga
     3181 ggcgatccgc gatgcgattg agcgtggcca ccagtttggg gccgtcttcc cggagcgccc
     3241 gcatgaattc ctcggcatgc gccaccgtgg ccttcacacc ttcgcgattc tccgcgacga
     3301 tgtgatccaa gttctccgcc acgttgcgaa aatgcgagac gatctccttg agcgagcgtt
     3361 ccccctcgcc agttccgagc gcattgcgca gcgatgcggt cacggccttc acgtcgtccg
     3421 cgatcgcgcc gaaccggttg atcaacgtat ccaagtccgc ggtgacctcg gcttgaggga
     3481 gttcggcgcc gtcgcccacg aatccgggct cacggccggg aagaatctcg acgtacttgt
     3541 cgcccagcaa accggcggcc ttgaccacgg ctcggctgcc gttggggatt cgatatcccg
     3601 gccggatccg catcgagacc ttggcgcggg agtcttccaa cgccattgct tcgacttcgc
     3661 cgatcggcac gcccgccatt ttgaccgggg cgttcttgtc cagtcccgcc gcggattcaa
     3721 agagggtgga aacccggaag ccttcgacac tccggaatgc gaaatctttg agttggaacg
     3781 acgcccagag aagagctccc agcgccagcg ccaccacggc gcccacgcgc gcttccacgc
     3841 tgaatccctt catgctccgc gctccgacgg ttgggcacta ggataacgga caaatccgtg
     3901 gatgtccttc gacacgccca ggagaatcgg atgtcgcgta cgtggtttga tgatcattcc
     3961 gttcgtggcg agcgcgttga accgcgtttc gtccgcttcc aacaatcggc tagaccgcaa
     4021 tgggaccctc cgcgctgccc gtgatgaact gtcggacgat cgggttgcgg ctcgcccgga
     4081 tttcgtccgg cgtgccgacc tcttgaatcc ggccttggta caacatggcg atccggtcgg
     4141 cgatcttgta tgcgctggtc atgtcgtgcg taatcgcgat cgacgtcatt ttaagttggt
     4201 gtttgagtcg aatgatcaac tcattgatca cgtcggccat gatcggatcc agacccgtgg
     4261 tcggctcgtc gtacagcatg atttgcgggt cgtgcgcgat cgcgcgcgcc aaccccaccc
     4321 gcttcttcat cccgccggac aattcggcgg gcatgaggtc ctccaccccc ttgagcccga
     4381 ccaacgcgag tttctcccgc gcgatctctc gaattgcggc gggcgagcgt ttggcgtgct
     4441 gcgtcagcca gaagcccacg ttttcccaga cggacaacga gtcaaacagg gccgcgcctt
     4501 gaaacaacat gccgaacttt ttgcgcaact ccgtcaattg cgtttcattg agggcgccca
     4561 cttcctggcc gtccaccacg acccgccctt gatccggctt catcagaccg atcacgtgtt
     4621 tgagcagcac gcttttcccg gtgccgctgc cgccgatgac caccatggtt tccccgcgct
     4681 cgatggtcaa ggtcaacccc gcgagcacga ccttggcgcc gaagctcttg tgcaggtcct
     4741 cgatctcgat catgtcaata gagcagcgcc gtcataatgt aatccgaaat caaaatcaag
     4801 acgcacgaga cgaccaccgc ccccgtggtc gcttgtccca cgccctccgc gccgcctctg
     4861 gcgtgaaatc cgcgttgaca actgaccaac gcgatgatgc cgccgaacac gatggccttg
     4921 agcaacccgc cgtacacgtc ggccacctcc aggtagtcga acgtgcgctt catatagatg
     4981 gtcgggttcg cgtcgagcac gaacaccgag actcccaacc ccccggcaac gccgatgatg
     5041 tcggaaaaca ccgtcaacac gggcagcatc aacaggcccg cgagaaaccg cggcagcacc
     5101 aaatacttga ccgggttcac gcccatcgac gccagcgcgt cgacctgctc ggtcacgcgc
     5161 atggtcccga gctcagccgc catggccgca cccgcccgcc ccgcgaccat caaagccgtg
     5221 aggaccggtc ccaactcgcg ggtcatcgac aaggcgacca ccgtgcccac cagactttcg
     5281 gcctggaacc gctgaaagcc ggtgtagctt tgcagcgcca gcaccatccc cgtggagagg
     5341 ccggtaatgg cgaccaccgg aatcgacttg gttcccacct cctccatctg tttgatatgc
     5401 agccggaagt ccaacggcct gaccagcgcg cctcgcacca cgttccacaa caacagggac
     5461 agattgccga actcgtggag cagcgtgatc gccgacgctc ccagccgttc caaccatcgg
     5521 atcatcgcga gcccgccccc tcgccgttga gataccgacg gggcacccgc ggtccgatcc
     5581 cgcagagcag ttcatacgcg atggtgcccg cggtctcggc cacgtcttcc gctggcaacg
     5641 acgccccgtc ctgcgatccg atcaagacca cctcatcacc ttccgccacg ccgggcacgt
     5701 cggtggcatc gaccatggtg aggtccatgc acacgcggcc ggcaaccggc gccttccggc
     5761 cgcggatcag caccgcgccg cggttggaca acgcgcgcgg gtacccgtcc gcgtacccga
     5821 tcggcagcac ggcgatgagg ctctcccgac gcgtcacgaa cgtgcggccg taactgatcg
     5881 gcgtgttcgg cggcacccgc ttgagcagca ggatggtggt cgtcagcgac agggcgggtt
     5941 tgagggcgac cccgtccacg tccggacccg gacggcaccc gtacaacatg attccgggac
     6001 gcgcggcgtc gtacaacggg ccgtcgctgg gccgcccgcc cggcgcggcc ccgagcgatc
     6061 gcgtcccctt gatcaacgcg gcgctgttgg ccgcgtgccg aatcgggatc ttgatcccgg
     6121 cgcgcgcgac cgcctccgac acggcatcga accgcgcgat ctgttcgcgg gcgtaggcgg
     6181 ggtctgcgag gtccgcgtcg gcgaagtggg tcatcagccc gtcgaggcgc aggccggggc
     6241 gttgggcgac ccgctcgacg gcctgcgccg cggcctcgac cgggaacccc agacgcccca
     6301 tcccggtatc gactttgacg tgcaccggcg agacgcggcc ggcgcgcacg gcggccgcgt
     6361 ccaacgcggc cgcgtgctcc gggtgaaaaa tcgcggccga cagccccaga tccaccacgt
     6421 cgcgggcctg ttcaggcgcg aagccgccca ggacgatgat gtccccgcgc acaccggcct
     6481 cgcgcaggac gcggccctct tcgaccaacg ccacgccgaa ccgccgcacg ccgtccgcct
     6541 cgatcgcccg cgccaccgcc gcggcgccgt ggccgtacgc gtcggccttg accacggcga
     6601 tcaggtgccg tccgccggcc aacgtccggg cctggccgac gttgaaacga atcgccccga
     6661 gatcgacccg cgcgacggtc ggccgcaggg cgggcgtggc atgggtcact gggtggccgc
     6721 cttgcgaatg gcgtcggcct gctgcgcggc ggccttggcc tccgcggcaa gccccttgga
     6781 cgcgaacagt tcggccttcc ggtcgaacag gcgcgcgcga agctccgcgt cgccaaccgg
     6841 cgccaacgcc tcggcttcgt cgatcacctt gcccgcttcg tagggcttct cccgggcaat
     6901 cagcacctgc gccagcgtgt cgaggtaata cggcgcccga tccgggtccc agcgcaacgc
     6961 gccgcgcacc agcgattccg cgggttcgac cgcgccctcc tggaacaact tctgacttct
     7021 ggcgtgcgcg gtctgcgcga aggcgcgcgc cttcgcggca aactcggcgg ccgcggccgc
     7081 cccggtccgt gcctcgtcga tcacgcgcac ggcctcctcg ggtcgccccg agtccaataa
     7141 gacctgagcc aggagaaccg gatagtccgc cgtgcgctcg gtatccctcc gaacggcctc
     7201 gcggagcagg cgctcggcct cgtcgtaccg ccccgccgcc gccttcgcct tgccctgctc
     7261 aaacgccaaa cgggcaatct cctgatggtc gtccgcgagc atgtcccgca ccttcgccaa
     7321 caacgacgcg tcgtcccgcc actgattcag cccgtcgtcg atcgcccggc tggcgtcaag
     7381 cggtttgccc agtttgagca acacgtccac gagcgtatcg aggtactccg gtcgcgggtc
     7441 ccacgccaac gccttacgcg ccagggattc ggcgctcgcg agccgcttgc cctgcgtgag
     7501 gtacaaccac gccaggttgt tggcgccggg agcggcgagc ggcgccccgg tgcgaaccgc
     7561 gcgggcatac cacgcctccg cgtcggcata ggcgcccgcg ttcgcggcga ggtttccgag
     7621 attcaccagc gcctgcgcgt gatccggctg ggcgcgcagg gccgatttat actcgtcggc
     7681 cgcgcggtcg agttttccgt ctcgctcgta cgccacgccg agcgccacgt gttcgtcggg
     7741 gctgagaggg tcgtggagcg tcacgacgtg cacccctccg agcactccgc agcccgacgc
     7801 gaccagcagc cacccgaccg cgagcgcgac cgaccccgcg gaccgccgca tgatccacgc
     7861 cggcacgatg cggttcacga gccgggcgcg accgccgagt cgcggcgttc ggttggcgcc
     7921 aatgcccacc atcccccgcg gccccaggac gacgcgaacc gtcggtaaga catggtggcg
     7981 ccggcgtcgc gcccggaata cagcaggacg cgttcatcgg catcgtcata ccccaccacc
     8041 acgacgaagt gcccttgccg cacgggtccg aatccgagat cgaggaacgc gatcacgggg
     8101 tggtcgtcgg ccacgcgcgc gcgcagccac gccagggtcc ccgcctcggc gcgaacggc
//
LOCUS       DBNGTT010000127         2196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2581295, whole genome shotgun sequence.
ACCESSION   DBNGTT010000127 DBNGTT010000000
VERSION     DBNGTT010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2581295"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="AC16BC_02910"
     CDS             complement(<1..355)
                     /locus_tag="AC16BC_02910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013662.7"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HIR3240597.1"
                     /translation="MSAVTTILLEGVPPGGSLDLAAYEKIGGYEALRKAVAGLAPADI
                     VEEIKRSGLRGRGGAGFSTGKKWEMALGKRGRLKYVCCNGSEGEPGTLKDRHLIRANP
                     HVLIEGVILAAYTTGA"
     gene            complement(418..588)
                     /locus_tag="AC16BC_02915"
     CDS             complement(418..588)
                     /locus_tag="AC16BC_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240598.1"
                     /translation="MARENFLDLNDDTGTLAFAGWRCMICGEILDAVIMSNRTSRPAP
                     LHNRNRKFLASR"
     gene            complement(816..1754)
                     /gene="mdh"
                     /locus_tag="AC16BC_02920"
     CDS             complement(816..1754)
                     /gene="mdh"
                     /locus_tag="AC16BC_02920"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR3240599.1"
                     /translation="MGRRKISVIGAGNVGGTTAQRLAERDLGDIVLLDINEGVAKGKA
                     LDIMESAPLEGFDARVIGTSNYDETTDSSLVVISSGVPRKPGMSRDDLLRINAEIVQS
                     VVREVAKRSPNAVLIIVSNPLDAMSYVAFRESGFPRERVMGMAGGLDSARLRAFIAME
                     LGVSIANVHALVLGGHGDTMVPLMRYTTVNGIPVEQLIPAQRLEALIKRTREGGAEIV
                     NLLKTGSAFYAPSAAAVEMAEAVLADRHKVIPSAVYCDGEYGIRGLFVGVPARLGARG
                     VESIVQVELTAAERALLDQSAAAVRELCEKVDAARK"
     gene            complement(1880..>2196)
                     /locus_tag="AC16BC_02925"
     CDS             complement(1880..>2196)
                     /locus_tag="AC16BC_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3240600.1"
                     /translation="TECCGFHTAAHDERVAIKLTGQHIKSARKNGAATMVTPCPLCHM
                     SLDIYQERAGEQRGERLDMPVFHLPQLVGLALGLSEKDLGLSRHLAPVAKALDQLTSP
                     QP"
BASE COUNT          379 a          762 c          682 g          373 t
ORIGIN      
        1 ccgcgccggt ggtgtacgcg gcgaggatca cgccctcgat caacacgtgg gggttggcgc
       61 gaatgagatg gcggtctttc agggtgcccg gctcgccctc cgaaccgttg cagcacacgt
      121 acttgaggcg cccgcgtttg cccaacgcca tttcccactt cttgcccgtg gaaaaccccg
      181 cgccgccccg cccgcgcagg cccgagcgct tgatctcctc gacaatgtcg gcgggagcca
      241 agccggccac ggccttgcgc aacgcctcgt acccgccgat cttctcgtag gccgcgagat
      301 cgagcgaacc gccgggcggc accccttcca gtaaaatcgt cgtgacggct gacatggcgc
      361 ggcgaaacct ctagcgcgga tccgcatcca cgccgacgcg ccgggcgcgg agcgccgtta
      421 gcgggacgcg agaaacttgc ggttgcggtt gtgaagcggg gccggacggc tggtgcgatt
      481 ggacatgatc acggcgtcca ggatttcgcc gcaaatcatg cagcgccacc ccgcgaacgc
      541 cagagttccg gtgtcgtcgt tcaagtccaa aaaattttcg cgggccatgt gaccctggca
      601 acgagggcaa tccattccag gctccccaag cgacctcgct ccggcagggc cgccccccgg
      661 tccgtgtgat gacggatatc gggatacgac cgcccgagac aagaatccct atggagagat
      721 tatgaagcaa acctaatgcc atgattgttc gttccctgga gtacaacgaa gccgccgcgc
      781 ggacagcccg cggcggctcg aggataaaac ttttcctatt ttcgggcggc gtccactttt
      841 tcacacaact cgcgcaccgc cgccgcggat tgatcgagca acgcgcgctc cgccgcggtt
      901 aattccacct ggacgatcga ctccacgccg cgcgcgccga gccgcgccgg cacgcccacg
      961 aacagaccgc ggatgccgta ctccccgtcg cagtacaccg cgctgggaat caccttgtgc
     1021 cggtccgcca acaccgcctc ggccatttcc accgcggcgg ctgacggcgc gtagaacgcg
     1081 ctcccggttt tcagcaaatt gacgatctcg gccccgccct cccgcgtccg cttgatcagg
     1141 gcctccaatc gctgcgcggg gatcagttgt tcgaccggga taccgttgac cgtggtgtac
     1201 cgcatcagcg gcaccatggt atcgccgtga ccgcccagca ccagggcgtg cacgttcgcg
     1261 atcgacacgc ccaactccat ggcgatgaac gcgcgcagcc gcgccgaatc cagcccgccc
     1321 gccatgccca tcacgcgttc cctcgggaac ccgctctcgc ggaacgccac gtacgacatc
     1381 gcgtccaggg ggttggacac gatgatcagc accgcgttcg gcgagcgctt ggcgacctcg
     1441 cggaccacgg actgcacgat ctcggcgttg atccgcagca agtcgtcccg gctcatcccg
     1501 ggtttgcgcg gaacgcccga ggaaatgacc accagcgacg agtcggtcgt ttcgtcgtag
     1561 ttcgaagtgc cgatgacgcg cgcgtcgaac ccctcgaggg gcgcggactc catgatgtcc
     1621 agcgccttgc ccttggccac gccctcgttg atatcgagca gcacgatatc cccgaggtcg
     1681 cgctccgcca ggcgttgcgc cgtggtcccc ccgacgttgc cggctccgat cacgctgatt
     1741 tttcgacgtc ccatctcccg ctcctccgga cgttccgacc cagccgccca agagcggcca
     1801 tcttagccca ccggcgggga attctgcaag cgagcgggga tcggtcccgc cgaaggattt
     1861 ttcgagattg ggagatgact tacggctgcg gcgacgtcag ttggtcgagg gctttggcga
     1921 ccggggccag gtggcgcgag aggccgaggt ctttctcgga gaggccgagc gccaggccca
     1981 cgagctgcgg gaggtggaag accggcatgt cgaggcgttc gcctcgctgc tcaccggcgc
     2041 gttcctggta aatatccaaa ctcatgtgac agagcgggca cggcgtgacc atggtggcgg
     2101 caccattctt gcgcgccgac ttgatgtgct ggccggtcag cttgatcgcg acacgctcgt
     2161 cgtgagcggc ggtatggaac ccgcagcact cggtcc
//
LOCUS       DBNGTT010000128         3882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1175813, whole genome shotgun sequence.
ACCESSION   DBNGTT010000128 DBNGTT010000000
VERSION     DBNGTT010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1175813"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="AC16BC_02930"
     CDS             complement(<1..249)
                     /locus_tag="AC16BC_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240601.1"
                     /translation="MNGFWWKTWVVFLGVALAMPGAAYAERPFLATEHATLLEHGEAR
                     FDFGVEAARFSSRTTRYTLKSELTSGILPNLNFRVEAPY"
     gene            382..792
                     /locus_tag="AC16BC_02935"
     CDS             382..792
                     /locus_tag="AC16BC_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3240602.1"
                     /translation="MEQAVRENINVLVVDDEVGPRESLKMILKPFYHVYTAENGNDAL
                     ALMKVHKIDLVTLDLKMPGLHGTDVLQAIKREHGETEVVIVTGFGGLKTAVDGIRFGA
                     SDYLLKPFNITEMLTTIRRVLDRRQSPPSLAANA"
     gene            complement(789..2534)
                     /gene="recN"
                     /locus_tag="AC16BC_02940"
     CDS             complement(789..2534)
                     /gene="recN"
                     /locus_tag="AC16BC_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR3240603.1"
                     /translation="MLCDLRVQRFALIDDLQLAFGPRLNVLTGETGAGKSILLDAVTL
                     VLGGRASPDMVRSGCEDAVVEASFDVAANSPAAAAFVDMGYPPDEDLVVQRVVLATGK
                     GRVYLNGRPVTASVLAQVTGHLIDLHSQHDYQSLMRADTPLTVLDAFAKADDLRRECR
                     VAWSAWTELDQALSSQSARATDRAREIALLTHEVEELDRAELAPGEADALERERTVLR
                     HAERLLAASRAAQERLDDQGVLEGAGAIAAELKAAAGLDPKLAEASELAASALVQLQE
                     LASTLRRYSDGGGPEEGRLDAIESRLAELQRLRRKYGVESEELADLAARKREQLRTLE
                     TEVESSHTLGARVDETRRRLETLADQLHGRRVEAAEKLASLVDEELARLRMSARLHVE
                     VAPMDDQGGIGPLGRDRADFLIRTNPGEPAKSLRKVASGGELSRVMLGLKSVLASVDA
                     APTLIFDEADAGVGGAVAEVVGRRFRAIADHRQVLCVTHLPQVASGADTHILVEKTVD
                     RGRTNTKARRLTDRERIREVARMLAGEEITATALRHAEEMIRLVSAPPVETQAPKGRS
                     PRGDSVTRPGARVSR"
     gene            complement(2564..2893)
                     /gene="trxA"
                     /locus_tag="AC16BC_02945"
     CDS             complement(2564..2893)
                     /gene="trxA"
                     /locus_tag="AC16BC_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011373481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3240604.1"
                     /translation="MGQAVKVETASWDATVLKAKGLVMVDFWAVWCRPCQMIAPIIEE
                     LAKEYDGRLTVAKLNTDENPDLASRYQIMGIPTLMFFRDGKAIEKIVGAASKQQFKEK
                     IDTLLAS"
     gene            complement(3047..>3882)
                     /gene="groEL"
                     /locus_tag="AC16BC_02950"
     CDS             complement(3047..>3882)
                     /gene="groEL"
                     /locus_tag="AC16BC_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3240605.1"
                     /translation="RGTLQVCAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGLKLEN
                     VKVTDLGRAKRVTIDKDNTTIVEGAGDSSKIQGRVKQIKAQIEETTSDYDREKLQERL
                     AKLVGGVAVINVGAATETEMKEKKARVEDALHATKAAVEEGIVPGGGVALLRCLPALD
                     KLKLEHDQQIGVNIIRRSLEEPIRQIVANAGLEGSVVVDKVRNSSKASQGFDASSETY
                     VDMIEAGIIDPTKVTRTALQNASSVAGLMLTTEVMVAEIPEKEPKAPAMPGHGGMGDM
                     Y"
BASE COUNT          674 a         1358 c         1138 g          712 t
ORIGIN      
        1 gtacggcgcc tcgacccgga agttcagatt gggcaggatg cccgaggtga gttcgctctt
       61 gagcgtgtac cgcgtggtgc gcgaggaaaa ccgggcggct tccaccccga agtcgaaacg
      121 cgcctcccca tgttcaagga gcgtcgcgtg ctcggtcgcc agaaaggggc gctccgcgta
      181 cgccgctcct ggcatcgcca gggcgacgcc cagaaagacc acccaggtct tccaccagaa
      241 cccgttcatg cgggcggatt gtacgggttt cgatccacag cgtcaagaaa ccgctcgacg
      301 ttccatcttc ttttgctatg cttagcgcca tcgcacgcgg gacgaacccg acaatccaca
      361 tcgaccgaga agggggcgca catggaacag gctgttcgcg aaaatatcaa cgtgttggtc
      421 gtcgacgacg aggtcggccc gcgcgaatca ctcaagatga tcctcaaacc cttttatcac
      481 gtttacaccg cggaaaacgg gaacgatgcg ctcgcgttga tgaaagtcca caagatcgac
      541 ctggtgacgc tcgacctcaa gatgccgggg ctgcacggga ccgatgtgtt gcaggccatc
      601 aaacgcgagc acggcgaaac ggaagtggtg atcgtaacgg gtttcggcgg actgaaaacc
      661 gcggtcgacg ggattcgctt cggggcgtcc gactacctgc tcaagccgtt caacatcacc
      721 gagatgctca ccacgatccg gcgggtgctt gaccgtcggc aatctccgcc gtccctcgcc
      781 gcgaacgcct aacgcgacac ccgggccccg gggcgggtga ccgagtcgcc gcgaggtgac
      841 cgcccctttg gggcctgcgt ctcaaccggc ggagcggaaa ccaagcgaat catttcctct
      901 gcgtggcgga gcgcggtcgc ggtgatctcc tccccggcca gcatccgcgc cacttcacga
      961 attcgctcgc ggtcggtcag ccgccgggcc ttggtgttgg ttcgaccgcg atccacggtt
     1021 ttctcgacca aaatgtgggt gtccgccccg ctcgccacct ggggaagatg ggtgacgcac
     1081 aacacttgtc ggtgatccgc gatggcccgg aacctccggc ccaccacctc cgccaccgcg
     1141 cccccaaccc ccgcgtccgc ctcgtcgaaa atcaacgtgg gcgccgcatc cacgctcgcc
     1201 agcaccgact tgagccccaa catcacccgc gagagctcgc cgcccgacgc gaccttgcgc
     1261 aacgatttgg cgggttcacc cgggttggtc ctgatcagaa aatccgcgcg atcgcgaccg
     1321 agcgggccga tcccaccctg atcatccatt ggcgcgacct cgacgtgcag cctggccgac
     1381 atacgcaggc gcgccaattc ctcgtcgacc agtgacgcga gtttctccgc cgcttcgacg
     1441 cggcgcccat gcagttggtc cgcgagggtt tccagccggc gccgtgtctc gtcaacgcgc
     1501 gctcccaacg tgtggctcga ctccacctcg gtttcaagcg tgcgcagttg ctcgcgcttg
     1561 cgcgcggcca ggtccgccaa ttcctcggac tccaccccgt attttcttcg cagccgctgg
     1621 agttcggcca ggcgcgactc gatcgcgtcc agccgcccct cctcaggccc acccccgtcg
     1681 ctgtatcgtc gcaacgttga cgcgagttcc tggagctgca ccagggccga cgccgccaac
     1741 tcggacgcct cggccagttt cggatcaagc ccggccgcgg ccttcaattc ggccgcgatc
     1801 gcgccggccc cttccagcac gccctgatcg tccagccgct cctgcgcggc ccgcgaagcg
     1861 gcgagcaatc gttcggcgtg ccgcaacacc gtacgctcgc gttcgagcgc gtcggcttcg
     1921 ccgggcgcga gctccgcgcg atccagttcc tcgacctcgt gtgtcaacag ggcgatctcg
     1981 cgcgcccggt cggtcgcccg cgcggactgc gacgacagcg cctgatccaa ctcggtccac
     2041 gcggaccagg caacgcgaca ctcacgccga agatcatccg cctttgcgaa cgcgtccaac
     2101 accgtcaacg gcgtgtccgc gcgcatcagc gactgatagt cgtgctgact gtgtagatca
     2161 atcaggtgtc cggtcacctg cgccaggacg gacgcggtca ccggccgccc gttgagatac
     2221 acccgtccct tgccggtcgc caacaccacg cgctgaacca ccagatcctc atccggagga
     2281 tagcccatgt cgacaaatgc agcggccgcc ggggagttcg cggctacgtc gaacgaggcc
     2341 tcgaccaccg cgtcctcgca gccggatcgg accatgtccg gcgacgcgcg gcccccgagc
     2401 accagggtga ccgcgtcgag cagaatggat ttaccggcgc cggtctcgcc ggtcagaaca
     2461 ttgagacggg ggccgaacgc gagttgcaga tcgtcgatga gggcgaagcg ttggacccgc
     2521 agatcacaga gcatggcgcg ccctcacgaa ggggatgacg acgtcaggac gcgaggagcg
     2581 tgtcgatctt ttctttgaac tgctgtttgg acgcggcgcc gacgattttc tcgatcgcct
     2641 tgccgtcgcg gaaaaacatc agcgtgggga tgcccatgat ctgataccga ctcgcgagat
     2701 ccggattttc atcggtgttg agcttggcga cggtgagtcg cccgtcgtat tctttggcca
     2761 attcctcgat aatcggcgcg atcatctggc acggacggca ccacaccgcc caaaaatcca
     2821 ccatcaccag ccccttggcc ttcaacaccg tcgcgtccca cgacgcggtt tccaccttca
     2881 ctgcctgtcc catgtacccc tccgaccgtt tcggcacatc gtaacgtaac gaattcgagt
     2941 gtgtcaacgc gacagatccg aacgcccatc ttccgggatg gtgcgcaaaa aaaccgcggc
     3001 ccctcacatg aggggccgcg gtgcgtgcat ctgattgctg tggaacttag tacatatccc
     3061 ccatgccgcc gtgtccaggc atcgcggggg ccttgggttc tttttccggg atctccgcga
     3121 ccatcacttc ggtcgtcagc atcaagcccg ccacgctcga ggcgttttgc agcgcggtgc
     3181 gggtgacctt ggtcgggtcg atgatgccgg cttcgatcat gtccacgtag gtctcggagg
     3241 acgcgtcgaa tccctggctc gccttggagc tgttccggac cttgtccacg accaccgagc
     3301 cctcgagccc cgcgttcgcc acgatctgac ggatcggctc ttccaaggag cgacggatga
     3361 tgttcacgcc gatctgctga tcgtgctcca acttcaactt gtccagcgcc ggaagacacc
     3421 gcagcaacgc cacgccgccg ccgggcacga tgccttcttc cacggccgcc ttggtggcgt
     3481 ggagcgcatc ttccacgcgc gccttcttct ctttcatttc ggtctcggtc gcggcgccca
     3541 cattgatcac cgccaccccg ccgaccagct tggccaggcg ttcctgcagc ttctcgcggt
     3601 catagtccga cgtggtctct tcgatctggg ccttgatctg cttcactcgg ccctggatct
     3661 tcgaggaatc gccggcgcct tcgacgatcg tagtgttgtc cttgtcgatg gtgacgcgct
     3721 tggcccggcc caggtccgtg accttgacgt tctccaactt caaaccgaga tcctcggaga
     3781 tcacctgtcc gcccgtcagg atcgcgatgt cttccagcat ggccttgcga cgatcgccga
     3841 agccaggcgc cttcacggcg cagacctgca gcgtgccacg ca
//
LOCUS       DBNGTT010000129        15386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2709502, whole genome shotgun sequence.
ACCESSION   DBNGTT010000129 DBNGTT010000000
VERSION     DBNGTT010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 15386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15386
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2709502"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="AC16BC_02955"
     CDS             complement(<1..1354)
                     /locus_tag="AC16BC_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240606.1"
                     /translation="MTRRRADGTRRAATISAPSASSGQSVAVRHGLAAVLILGLVAIG
                     YGNSLDGAFHLDDQNVTADSALHLRELSPAALAGALWHGRPVASLTFALNYYWGEHRV
                     FGYHLVNVAIHAATALALYAFLVLLFDLPRTRERIGDLAAPLALGAALLWAVHPVQTQ
                     AVAYIVQRMTSLAALFYLLALVAYLRGRRAHGRPRVLWWIGALGCGALAIGSKEIAAT
                     LPVALFLIEWCLCEPDPRTVKRAMAVAGLLAIPILAVAALVVHDRAGGSFWAQLTTHR
                     SVIQSFTLTEHFLTEGRVIIHYLTLMVWPHPSRLSIEYDFPLSRGWFDPPTTAVSWLV
                     IAGLIAAGLGLRRRAPLAASGILWFFLQLAIESSVIPLDLVFEHRLYLPSMGLAILAA
                     AASAWVWQRWPERGARWIPAALAALLLVVWTGWTIQRNRVWETEWSLWTDAAAKAPTN
                     P"
     gene            complement(1564..2067)
                     /locus_tag="AC16BC_02960"
     CDS             complement(1564..2067)
                     /locus_tag="AC16BC_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240607.1"
                     /translation="MSAPGAARLSQCRARQQERDDVSHTAPRHLTPEDHSVRQRALAL
                     RKALLALHKALIDSERVGFEKTMGEIKSPNHFLQLLTGDPWFAWLHPLSRLIVALDEA
                     LDDKEPLTLAGAEALMSEFSRLLIPTEHGDGFSGHYFEALQRDPDVVLAHAEVTKHLA
                     QKRPSAA"
     gene            complement(2098..2526)
                     /locus_tag="AC16BC_02965"
     CDS             complement(2098..2526)
                     /locus_tag="AC16BC_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3240608.1"
                     /translation="MAVRSAEAVWTSGLKDGKGTMKLGSGAYEGAYSFASRFEHGTGT
                     NPEELIGAAHAGCFSMALSAGLERAGHPPRSVRTNARVHLEKVGDGFQITKIQLQTDA
                     DVPGITPEKFREQAEAAKQGCPVSRALAGVRIELDAKLTV"
     gene            complement(2699..3667)
                     /locus_tag="AC16BC_02970"
     CDS             complement(2699..3667)
                     /locus_tag="AC16BC_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240609.1"
                     /translation="MLEEKVWYIDDPDDPPIVSRTQTAAKPSRPQKNPAVAFSWSLVI
                     WGAGQFYNRQGAIGLLLLLLMVNCFATPALLWVSWDALPVLQIPISLTTSQFMLMIGT
                     IYLSGLIVWFVGAVQAYYRTSRARTAAFQGIANRFLPMFCSLLVPGWGQFLNGQAKKG
                     VFFLFIAVITFFAVPILVMILWAWPMLDAAEDRLFWEPVLIAVAALAPAALLIWPVAW
                     FDALVVSRDDTKKEPVLKRLEYANNRRRMYGLGRGVFPYFKQTLMLGLLLALFAIVTY
                     NYVPRDYYIARVQELRDASAEHHMVLIPRLLDRALHDVLQRRPGPG"
     gene            complement(4017..4538)
                     /locus_tag="AC16BC_02975"
     CDS             complement(4017..4538)
                     /locus_tag="AC16BC_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR3240610.1"
                     /translation="MADQRKRLPANASGNFFVDATCINLDAVRHVGASGVSGNFFVDA
                     TCINCDTCRQLAPTTFREVDDYSAVARRPQDDAELHRAYQALLACPTGSIGADVSDAA
                     RLQNAMAAFPSHLDRGVFYCGFNSEKSFGGNSYFIRHPGGNWLIDSSGCCRATVTAST
                     SRPPRCTRTSSVC"
     gene            4868..5407
                     /locus_tag="AC16BC_02980"
     CDS             4868..5407
                     /locus_tag="AC16BC_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240611.1"
                     /translation="MLKEAMAPVVPGLLMSFLAIVVGTGLGAAFGLFEDRIKDGLSAD
                     VQAHSQIHEQTPDNIKKQVGDGWRYYQRAHFHAQGLGALGVAIIVVLGLSWVSAPVKK
                     WLALATGLGALLYPFCWLLMGLRIGSVGKQAAHASVDWLAALSVPLFFGGMMVTLLIL
                     FLGWLFPRELPRGLRRFAA"
     gene            complement(5498..6313)
                     /locus_tag="AC16BC_02985"
     CDS             complement(5498..6313)
                     /locus_tag="AC16BC_02985"
                     /inference="COORDINATES: protein motif:HMM:NF026037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3240612.1"
                     /translation="MNALKFVTGVALSTVVALSVGSVRAEEYAVVPVPDGGALTGTVT
                     FKGTPPPPKQFDLAKFPNMAFCAKFDSDGEGHRLVQQVNLGKRNALKDVVVYMEVKQG
                     KPFDFTWVAVKADGCRFLAQGPSTLAGVVVKKKEVRIENMDADPSDPKAATGVLHNPH
                     GYEVYGSNNASLFNLPLPEKGQIIRKQVMLRKKDSVLKIECDQHNYMQVFFHPVDNPY
                     YAVVGDDGTFQIDQIPPGEYEVHAWHPMLGTQETTVTVAANGQATADFAFAAK"
     gene            complement(6611..7477)
                     /locus_tag="AC16BC_02990"
     CDS             complement(6611..7477)
                     /locus_tag="AC16BC_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240613.1"
                     /translation="MPINTATGWSDSPHITRDGNRLYFMYSRYDFGPWILSGGATAPV
                     LAGPDRPGLHHSANPFDESDVYLATRNPNGTWSEAVNLGFNGAYGDSSGMEINGGDAF
                     VWLQGNGATNNIVTAAKNPDGTWGAPVDLGPEVNDHGAGVFQDNPHLSPDGLGLWFTS
                     NRAGGTGARDIWFSLYASGNWSSPVNMGAPFNSAGDEDQIWIAPVGANPDIYWNGPAG
                     INHCTWTGATCAAPPDAVTIPGCAVAAEVSVTDDGQTMYFACGNPTTGRVSIMYSVKQ
                     PGGGWGTAVPVD"
     gene            8182..9540
                     /locus_tag="AC16BC_02995"
     CDS             8182..9540
                     /locus_tag="AC16BC_02995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3240614.1"
                     /translation="MWGRVVREATPDAAGTRERDGYLFGIAFTSDPASRVLSHAQWWL
                     PWLAIGLFIAGGVNVLYLKTFNVYYFWYKPIFNAYSLLISIYIVSRFALASLYRPPRD
                     VGHCPSVSAVIVCKNEEASIGRTVDCIARSFYPADRLELIAVNDGSTDATLAEMERAR
                     ERYPELKIINFEKNRGKRHGMAAGARLARGEILVYVDSDTFVRRDAIRKIVQGFADPE
                     VGAVCGHANVANARKNLLTKMQEVRYFVAFRVVKAAESLFSTVSCCSGCLAAYRRSYV
                     MAILDRWQHQRFLGKEATFGDDRSLTNFMLRRHPVLYHSEAVCTTIVPETYSNFFRQQ
                     LRWKKSWLRESFLASLFMWRRHPFAALLFYLGVTFPICSPLIVANALVLPLVGYGDIS
                     TLYVYGSLLMAIMYGLVYLAKYRNGLWVYGVLFAVFYMLILVWQTYYALCTVRKNHWG
                     TR"
     gene            9544..11472
                     /locus_tag="AC16BC_03000"
     CDS             9544..11472
                     /locus_tag="AC16BC_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3240615.1"
                     /translation="MDTRADPLSARTFRRDRIAWYGLFVLLAVLGGGGYWAWNRTVVA
                     PPDLSRADSTDPRILVLAYDRVNNRGTETAVTPRALREQLEALRQEGFDPVTLQELTR
                     FYYEGRPLPPKPILLTFDFGYLDTYDAVDPVLRAMGWRAVMFLVTSRQARRDAAYLYW
                     DRVRRMVNSGVWEIGAQGHLSYDPIPLDERGAVGSFLLDRMWLGVAGRHESLAEFTDR
                     VRGDYAVSKQAIESNVRGDRMLACASRIGIAGTTTRDAFQANRDAMSSLCPLGFVEDR
                     LGVNDRLSDPRGLKRLRVNPDWSVGDIIQRLRAALDDPPFGSSDKHRAPPAWVADVGE
                     VSVEHGDLVMIGSPRVDLWLPGSQWPLDWVIEADLWSNGADVWLVQESDTPGKSWRWG
                     GNDRAIVLQRSQWGRPPETVATFPRGITSGVWHHVKLVKRGAGVWVEWDGRPLRKRPV
                     YLSGMGRGVVGVVGWRSEGGATVRMRHLTFSRYPFEMRSVSANPSEHEVTALIRDAPT
                     IAALSPLGGVMSGDRVQDVRFDRELLAILSHRYGWEVVPMIRVLPGREAAAFGASEGA
                     PSRPSVQWSATLLDYIERNGWSGIGLDLRHLPPSARPELGDGIDQLDRRLRAQGRRFV
                     QTTGGDKRLHASPAYASR"
     gene            11501..14560
                     /locus_tag="AC16BC_03005"
     CDS             11501..14560
                     /locus_tag="AC16BC_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240616.1"
                     /translation="MVTLGLIGTVALLTFASPAWAASPGQKALDKGVELYRNGRFTAA
                     ADAFREAIQRDPGLLKARENLGWAYWRAGRLAECRKVWTQYAALYPDRPEPFNLLTQL
                     HLAQKKYPAAIQTIKKSLALAPDQPKERLKLAKALLWDRQFDQAKRYAALHATAYPDD
                     VAIQVFWGEVLMRYHDFQAAEAQWRKVLALGPPSPRVRYYWIRALYNLGQYDAALLEA
                     RKAIATDGPQQDLLQLLIEDALARDDAAEAVRWYELAVEQFPERPAFWVELAELYRER
                     GMMRASDATLRRAKQRHPEHFEIFINVADNHRLGKRYDKALREYRELFARYPQNRDAF
                     IGLFNTLVEANHGHEALDLLEHNQTTFLTDSELDMDRARVLLAQGQTSRAQALFMRVA
                     SPEKQYVPIVLYHGLGGHPRSANLAVALFDSQLKALRDAGYTAITVRELAEMIDGRQP
                     FPRQPIVITFDDGRLDSFQLGDPVLAKYGMKATMFVPSAHMIDRHPFFADWDLIRRYA
                     RTGRWDMQGHGHEAHDPIPIDESGQTGGFLVNRQWLEADQRLETPEEYAERLEADYQE
                     TIRLLTRNVPNLEVVAYAFPYSEAGQSSVGNASDAATLNERLLFRYFRYGFVQDSIGY
                     NEVNPTVPTMLRRLNVPRSWDGERLLAHLATQHPRHKAQMHIGTSWMWAGQYGKSRRV
                     FEALAAENVLLKGPSEYQLAEVAFQEGQFREARRHLETAIEYGERSSRGEQLMARLRW
                     ENQPRVDARFGTSHDSDERVTWWNTLRVMYPLTNPVTLWTDVGVIRLTEDGLPALSGR
                     EVGVGTEWRGTGGLGLRAQLRHRSIEKSPNSLNVWLDGMYERGPHELRLRWASEDVDT
                     VQAHEKGLQSRGYTAAYRLRPTPNWDGRIQASIRSYDDGNDRVDWRTAITRRLPTQAQ
                     WRVGGALSYSDTGVQSLRYYTPEDLIVGRAIVAYRQRWESGWELAGEAGVGWADDALR
                     GNRMVSDVSLDALQVWGERLQSQLGWAYARNPGYQSWWLEGSVSLRF"
     gene            complement(14557..>15386)
                     /locus_tag="AC16BC_03010"
     CDS             complement(14557..>15386)
                     /locus_tag="AC16BC_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR3240617.1"
                     /translation="LPVESAVARVRAAGARPWLTIVNDVASGHGIALKDPQVVHDLLS
                     GEERRAAHRAAIMDLASRYGMSGVDIDYENLRARDRALFTTFVRELAADARRQGVLLS
                     VTVQPKLGDRWSDGSGAMDWGPLCATADRLQIMLYNLHSRATGPGPMATGRWIRGVLN
                     YANSQCDPKRVVPVLKVSGMRWADGTVEGIQYDQAIALAQEHGASISRDADDQVPYFT
                     YRVNEQPHTVYYEDAVSLTEKLEVIRSLGYRTVLLWSLGRQDPALLPPFAAEESRGR"
BASE COUNT         2746 a         4751 c         5079 g         2810 t
ORIGIN      
        1 ggggattggt cggcgccttg gccgcagcgt cggtccagag cgaccattcg gtctcccaca
       61 cccggttgcg ctggatcgtc catccggtcc agaccacgag aaggagcgcc gcgagcgcgg
      121 cggggatcca ccgcgcgccg cgctcgggcc atcgttgcca cacccacgcg ctcgccgccg
      181 cggccaggat ggcgaggccc attgacggga ggtacaggcg gtgttcgaac accaggtcga
      241 gcgggatcac gctcgattca atggcgagct ggagaaaaaa ccacaggatg ccggacgccg
      301 cgagcggggc gcggcgccgc aaccccaggc ccgcggcgat cagcccggcg atcaccagcc
      361 aggacacggc cgtggtgggc gggtcgaacc agccgcggga cagggggaag tcgtactcga
      421 tgctgagtcg cgacgggtgc ggccagacca tgagggtcag ataatggata atcacgcgtc
      481 cttcggtgag gaagtgttcg gtgagggtga agctctggat cacggatcgg tgcgtggtca
      541 actgggccca gaacgacccg cccgcgcgat cgtgcaccac cagcgcggcc accgccagga
      601 tgggaatcgc caacaggccc gcgaccgcca tcgcgcgttt cacggtccgt gggtccggct
      661 cgcacagaca ccactcgatc aagaagagcg cgacggggag cgtggccgcg atctccttgc
      721 tgccgatggc caacgccccg caccccaggg ctccgatcca ccataagacg cgaggccgcc
      781 cgtgggcgcg gcggccccgg aggtacgcga ccaacgccaa cagatagaac aacgccgcga
      841 ggctcgtcat gcgctgcacg atgtacgcga cggcctgggt ctgcaccggg tgcacggccc
      901 agagcagggc ggcgcccagc gcgagcggcg cggccaggtc gccgatgcgc tcgcgcgtcc
      961 gcgggaggtc gaacagcagc accaagaacg catacagcgc caaggccgtg gccgcgtgga
     1021 tcgccacgtt gaccaggtgg tacccgaaga cgcgatgctc gccccaatag taattcaacg
     1081 caaaggtcag gctggccacg ggtcgcccgt gccacagggc gccggccaac gcggcggggc
     1141 tgagttcgcg caggtgcaac gccgagtcgg cggtcacgtt ctgatcgtcc aggtggaatg
     1201 ctccgtcgag actgttgccg tagccgatcg ccaccagacc aagaatcagc accgccgcga
     1261 gtccgtgccg gaccgccacg gattgacctg acgatgcgct cggggcggag atcgtggcgg
     1321 ctcggcgagt cccgtcggct cggcggcgag tcatcaccgt aatccttccc ggttctgcgt
     1381 gtcgtgcgtg gtcatggttt gtgcggaccg cggtaaacgc cctacggtag ccaaagcctg
     1441 ccgcccacgc aagtcccgtc aggatcgtcg cgtatcgagt tggagaggtt cggtccgaaa
     1501 ggatgacgga atgacgtgcc gcgacggacc gggacggatc cgtcgggaaa aaatcctgta
     1561 ccgctacgcg gccgacggtc ttttctgcgc gaggtgcttg gtcacctcgg cgtgcgcgag
     1621 caccacgtcg gggtcgcgtt gcagcgcttc gaagtaatgc ccggaaaacc cgtccccgtg
     1681 ttccgtgggg atgagcaggc gactgaattc actcatcaat gcctcggccc cggccagggt
     1741 cagcggctcc ttgtcgtcca gcgcttcgtc cagcgccacg atcaggcgcg acaacgggtg
     1801 cagccaggcg aaccacgggt cgccggtgag cagttgcaga aaatgattgg gcgacttgat
     1861 ctcgcccatg gttttttcaa agcccacgcg ttcggagtcg atcagcgcct tgtgcagggc
     1921 gagcaacgcc ttgcggagcg caagcgcgcg ttgacggacc gagtgatctt ccggcgtaag
     1981 gtggcgtggt gcggtgtggc tcacgtcgtc tctctcctgt tggcgcgccc ggcattgcga
     2041 caaccgcgcg gcgccgggcg cggacaccct atcacatccc cgcgcgttgt gccggggcta
     2101 gaccgtcagc ttcgcgtcca gctcgatccg cacgccggcc agggcacgcg agaccgggca
     2161 gccctgcttg gccgcctcgg cctgctcgcg aaatttttcc ggggtaatgc cgggcacgtc
     2221 ggcgtcggtt tgaagctgga tcttcgtgat ctgaaagccg tcgccgactt tttccagatg
     2281 cacccgggcg ttggttctga cgctcctggg cgggtgtccc gcgcgttcga gcccggcgga
     2341 gagcgccatg gagaaacacc cggcgtgcgc cgcgccgatc aactcctccg ggttggtccc
     2401 ggtgccatgc tcaaagcgcg acgcgaacga atacgcgcct tcgtatgcgc cgctgcccaa
     2461 cttcatcgtg ccctttccgt ccttcaaccc cgacgtccat acggcttccg ctgatcgaac
     2521 ggccataacg tcctccaggt gtgacgctgc tccagacgac tccgtcccaa ccagccggag
     2581 tatatggttc ttgaatggaa atttcccggg cgtggaaatg gaggccgaaa cggtggctgt
     2641 cggctgagtg accaggtggg tgagtcagtc gtcgcgaggg tacgggctgc gaatggcgtt
     2701 aaccagggcc gggtcggcgt tgcagcacgt cgtgcagcgc gcgatcgagc aggcgcggga
     2761 tgaggaccat gtggtgctcg gccgacgcgt cgcggagttc ctgcacgcga gcgatgtagt
     2821 agtctcgcgg gacgtagtta taggtcacga tcgcaaacaa ggccaagagc aatccgagca
     2881 tgagcgtctg cttgaaatac gggaacacgc ctcgtcccaa gccgtacatg cgccggcggt
     2941 tgttggcgta ctccagtcgc ttcagcacgg gctctttctt ggtgtcgtcc cggctcacca
     3001 ccaaggcgtc aaaccaggcc acgggccaga tcaacagcgc ggccggcgcg agcgccgcga
     3061 ccgcgatgag cacgggttcc cagaacagac ggtcctcggc cgcgtcgagc atgggccagg
     3121 cccagaggat catcacgagg atgggaacgg caaaaaacgt gatgacggcg atgaacagga
     3181 agaacacgcc cttcttggcc tggccgttca gaaattgtcc ccaacccggg accagcaggg
     3241 agcaaaacat ggggaggaac cgattggcaa tcccttggaa tgccgcggtt cgcgcgcggc
     3301 tggtgcggta ataggcctgc accgcgccca cgaaccacac gatcaggccc gacaaataga
     3361 tggtgccgat catcaacatg aactgggacg tggtcagcga aatcgggatc tggagcacgg
     3421 gcagggcgtc ccaagacacc cacaacaagg cgggcgtcgc gaaacaattc accatcagca
     3481 ggaggagcag caacccgatc gcgccctggc ggttgtagaa ctgtccggcc ccccagatga
     3541 cgagcgacca ggagaacgcg acggccggat ttttttgcgg ccgcgagggc tttgccgcgg
     3601 tctgtgttcg actgacgatc ggcggatcgt cgggatcatc gatgtaccag actttttctt
     3661 ccaacatgga ccggcctcca ccccctcccg tttgcgcggt cggaaatata gcaccgggtc
     3721 acgctggcgt cgccccggtt ttcccgcggc cgcgatgggg tctggggggg cgcgttcccg
     3781 cgagggcctc gatcggttgg gaacgccccc caaaggaccg tgggagcggc ctgcaacaag
     3841 gctgagcgag ggggagatcg tgcgatggat tggtcagaaa tgggcaacag gaactgcggg
     3901 gaaccgttcg tacctatttg atagggaacc tgttttcgga cggccccgag gcgctatcgc
     3961 caccggggcg ccccggcgtc acgccctgcg tcctgtcacg gtggttcgac ggcgggtcag
     4021 cagacgctcg atgtgcgcgt gcatctcggc ggccgggagg tggatgcggt caccgtggcc
     4081 cggcagcacc cactcgaatc gatcaaccag ttcccgccgg gatggcggat gaagtagctg
     4141 ttgccgccga acgatttctc cgaattgaac ccgcagtaaa acaccccacg gtccagatgc
     4201 gacggaaacg ccgccatcgc gttttgcagc cgcgccgcgt cgctcacgtc cgcgccgatg
     4261 gagccggtcg ggcaggcgag cagggcctga tacgcccgat gcagttccgc gtcgtcttgc
     4321 ggccgccgcg cgaccgccga atagtcatcc acctcccgga aggtcgtcgg cgcgagttga
     4381 cggcacgtgt cgcagttgat gcaggtcgca tccacgaaga agttcccgga gacgccgctc
     4441 gcgccgacgt gtcgcaccgc gtcaaggttg atgcaggtcg catccacgaa gaagttcccg
     4501 gaggcgttgg cggggagacg ttttctctga tcagccatcg tcgaatcgcg gttcgcaggc
     4561 tctctggttc gtgtggatga tcccattata cgggcggcct tgagaccatg tcagggcgcg
     4621 acctaaccac aaatttcccg aaaacgattc tttcggggca tagccacgcc cccctgattg
     4681 cctccatcat ggcggtcaat gcgtgcggct ccctcgtacc ggcgatggac aacaacggag
     4741 accggaacgt tgacgcggcg attcccgcgg tgtgggtcga tctgaacgag gccaccggga
     4801 tcgggaaggc cgtgccggcg aagcttgccg gataaacaga gccagtgatc aagaggggag
     4861 gatgtcgatg ttgaaagaag ccatggcgcc ggttgtcccc ggcctcttga tgtcgtttct
     4921 ggccattgtc gtgggcaccg gattgggggc ggcattcggt ctctttgagg accggatcaa
     4981 agacggcctg tccgcggacg tgcaagccca ttcgcagatc cacgagcaaa cgccggacaa
     5041 tatcaagaag caggttggag acggctggcg ctattaccag cgcgcgcact ttcacgcgca
     5101 ggggctgggc gccctggggg tcgcgattat cgtggtgctg ggactgagtt gggtctcggc
     5161 cccggtcaag aaatggctgg cgctcgcgac cgggctcggg gcgttgctct atcccttctg
     5221 ctggttgctc atgggactcc gcatcggttc ggtgggcaaa caggcggcgc atgcgtcggt
     5281 cgactggctc gccgcgctga gcgtcccgct ctttttcggc gggatgatgg tgacgttgct
     5341 gattctgttt ctgggctggc tgttcccgcg ggagctgccc agagggttgc ggcgattcgc
     5401 cgcgtaagcc gaccgccacc cacgactgcg cggcatcgat catcgggcgc gaggttgccc
     5461 cggatgaccg ggacgctcgc ggtgcgtctg cagccggcta tttcgcggcg aacgcaaagt
     5521 ccgctgtggc ttgtccgttg gccgcgaccg tgaccgtggt ttcctgtgtg ccgagcatcg
     5581 ggtgccacgc gtgcacttca tactcgcccg gagggatctg gtcgatctga aacgtcccgt
     5641 cgtcgccgac aacggcgtag tacgggttgt cgaccgggtg aaaaaacact tgcatgtagt
     5701 tgtgttgatc gcactcgatc ttgagaacgc tgtccttctt tcgcagcatc acctgttttc
     5761 tgatgatctg tcccttctcc ggcaagggca ggttgaagag cgaggcgttg ttgctgccgt
     5821 acacctcgta gccgtggggg ttgtgcagca cgccggtggc cgccttgggg tcgctggggt
     5881 cggcgtccat gttttcgatt cggacttcct ttttcttcac caccactccg gcaagcgttg
     5941 aaggaccttg cgccaggaaa cgacaaccgt cggccttgac cgcgacccac gtaaaatcga
     6001 acggtttgcc ctgtttgacc tccatgtaga cgacgacatc cttgagggca ttgcgtttgc
     6061 cgaggttcac ctgttgcacc agccggtgac cctcgccgtc ggaatcgaac ttggcgcaaa
     6121 atgccatgtt gggaaatttc gcgaggtcaa attgcttggg cggaggcggc gtccccttga
     6181 acgtgacggt tccggtcagc gcgccgccgt caggcactgg aaccactgca tactcctccg
     6241 cccgcaccga cccgactgag agcgcgacca ccgtcgatag cgcgacgcct gtgacgaatt
     6301 tcagcgcgtt catgaccgtt cctccttgga ggagagacca cttcgtgatc gagagcggca
     6361 gagtacgtgt ggacagcgag tcccgggggg ctggcgtgcg gacgcggtcg agcgatgaag
     6421 aacgtcgacc aacggggcgg ctgatcgtga agtcagcatc gctggtcctc gggagggacg
     6481 tggtgtgagg atacgccatc agcgtatgaa aaggagttga ttgttgtcaa cgaggggccg
     6541 gtccataacc agacgtcgat cgatgatttc acgacgtcca cgaactccgg cgctctcagc
     6601 ccatgggttg ttaatccacc ggaacggccg tcccccaacc tccgcccggc tgtttgacgg
     6661 aatacatgat gctgacccgg ccggtggtcg gattgccgca cgcgaaatac atggtttggc
     6721 cgtcgtcggt cacggagact tccgccgcga cggcacatcc ggggatcgtg acggcatcgg
     6781 ggggcgcggc gcacgttgcc ccggtccagg tgcaatggtt gatgccggcg ggaccgttcc
     6841 agtagatgtc gggattggcc ccgaccgggg cgatccagat ctggtcctcg tcccccgcgg
     6901 agttgaacgg agcgcccatg ttgacgggag acgaccagtt cccactcgcg tataacgaga
     6961 accagatgtc tcgggcgccg gtgccgcccg cgcggttcga ggtgaaccaa agcccgagtc
     7021 cgtccgggga gagatgcggg ttgtcctgga agaccccggc tccgtgatca ttgacctcgg
     7081 ggcccaggtc aacgggggcg ccccatgtgc cgtcggggtt cttggccgcc gtgacgatgt
     7141 tgttcgtcgc cccgttgccc tgaagccaga caaaggcgtc gccgccgttg atctccatgc
     7201 cgctggaatc gccgtaggcg ccgttgaagc cgaggttgac ggcctccgac cacgtgccat
     7261 tgggattcct ggtcgccagg tacacgtcgg actcgtcaaa tggattggcg ctgtggtgca
     7321 ggcccggccg atccggtcct gccaacacgg gcgcggtcgc tccgccgctg agaatccacg
     7381 gcccgaaatc ataccgggaa tacatgaaat agagacgatt cccgtcgcgg gtaatgtggg
     7441 gagaatccga ccagccggtc gctgtattga tcggcacgcg cgtccgggac tccgaaccgc
     7501 cgctcgagca gcttgccaag gcccctgcgg tgagcgcgag agccaggcaa cgaatcccgg
     7561 cccgactccg ggatgcggca acgcgatggg atgtcaacgg atcagacctc ctggaggttt
     7621 cgcgcgacgc gattcacaaa tcataatata tcaacgctcg aaaaccggtc gatagggttt
     7681 cgcagccgcc gcacatagtc cggcgagaac tcgcgaattg gttgccccct accttcgtag
     7741 cgatccaacc cccaacacag actgagctac tacccaattt gagggatatc ccccgaattg
     7801 gcgtccacgt atactgcctg aggctgtgcc ttgcccgtcc cctcgatcct tccccgcgtg
     7861 ccaggaaggt cagtgacaag gacagcacct cctatggagg atgaaaacgt gttccgttct
     7921 cgcggtcctc tcgggactcg gcaaaacggg gaacaacggg catcccctcg ctctccgatg
     7981 ccattgcgaa cctgttgtgc caggctgaac cgtttgggat ttccccgtcg tgaaaccgca
     8041 ctgctcaccg attggaacgt gcggggaggg cgcctccatt ccagccgtcc cctcgacgtg
     8101 ggcgaacgcg tacgcgtgag gatggagccg acgccggctc agcgccaggc gattccgcct
     8161 ggtctccgga cacatcgctg gatgtgggga cgcgtggtgc gcgaagccac gccggatgcc
     8221 gcagggaccc gcgaacgcga cggatatctc ttcgggatcg cattcacaag cgatccggcc
     8281 tcgcgggtgc tgagccacgc gcagtggtgg ctcccgtggc tcgccatcgg gttgttcatc
     8341 gcgggcgggg tgaacgtgct ctacctcaag accttcaacg tctactattt ctggtacaag
     8401 ccgattttca acgcgtacag tctgttgatt tccatttaca tcgtctcgcg ttttgcgctg
     8461 gcgtctctct accgcccgcc gcgtgacgtg gggcactgcc cgtccgtcag cgcggtgatc
     8521 gtgtgcaaga acgaagaggc ctcgatcggg cggaccgtgg attgtatcgc ccgatcgttt
     8581 tatcccgccg atcgtctcga actcatcgcg gtcaacgacg gttccaccga cgcgacactc
     8641 gcggagatgg agcgtgccag ggagcgctat ccggaattga agatcatcaa ttttgaaaag
     8701 aaccgcggca aacgtcacgg catggcggcc ggagcgcgct tagcgcgcgg cgagatcttg
     8761 gtgtacgtgg actccgatac gttcgtgcgg cgcgatgcga ttcgaaagat cgtgcaaggg
     8821 ttcgcagatc ccgaggtagg ggcggtctgc ggtcacgcca acgtggcgaa cgcccgaaag
     8881 aacctcctga ccaagatgca ggaggttcgg tattttgtgg cttttcgcgt ggtcaaagcc
     8941 gctgaatcgt tgttctcgac ggtgtcgtgc tgctccgggt gtctcgccgc gtacaggcgc
     9001 agttacgtga tggccattct ggatcgttgg cagcatcagc ggtttctggg gaaggaggcc
     9061 acttttggcg acgaccggag tctgacgaat ttcatgttgc ggcgacaccc cgtgctctat
     9121 cactccgaag ccgtctgtac cacgatcgtc cccgaaacct actcgaactt cttccgccaa
     9181 caactacgtt ggaagaaatc ctggctgcgc gagagttttc tcgccagcct gttcatgtgg
     9241 cggaggcatc cgttcgcggc ccttctcttc tatctcggcg tcacgtttcc gatctgttcg
     9301 ccgttgatcg tcgcgaacgc actggtgctg ccgctggtgg ggtacggcga catctcaacc
     9361 ctctacgtct acgggtcgct gttgatggcc atcatgtacg ggctggtgta tttggcgaaa
     9421 tatcgaaacg gtctctgggt ctacggcgtg ctgttcgccg tcttctacat gctgatcctc
     9481 gtctggcaaa cgtactacgc gctctgcacg gtcaggaaga accattgggg cacgcgctga
     9541 gtcatggaca cccgggccga tccactgtct gcgcggacgt tcaggcgaga ccggatcgcc
     9601 tggtatgggc tgttcgtgct cctcgcggtg cttggtgggg gcgggtattg ggcgtggaac
     9661 cggaccgtcg tcgcgccccc ggatctatcc agggctgatt ccactgatcc gcggatcctc
     9721 gtgctggcgt atgaccgcgt caacaatcga gggaccgaaa ccgcggtgac ccccagggct
     9781 ctacgcgagc agcttgaggc ccttcggcaa gaaggattcg atcctgtcac gctgcaggaa
     9841 ctgacgaggt tctattacga gggccgtccg ctcccgccca agcccatcct cctgaccttt
     9901 gactttgggt acctcgatac gtacgacgcg gtcgatccgg tgttgcgcgc gatgggctgg
     9961 cgtgcggtga tgtttctggt caccagtcgt caagcgcggc gcgatgccgc gtacctctat
    10021 tgggatcgcg tgcggcgcat ggtgaacagc ggggtctggg agatcggggc tcagggacat
    10081 ctcagttacg atccgattcc tctcgatgag cggggcgccg tcgggtcgtt tttgctggac
    10141 aggatgtggt tgggcgtcgc gggacgacac gaaagcttgg cggagttcac cgatcgcgtg
    10201 cgtggagatt acgcggtcag caagcaagcg atcgagtcca atgtccgagg cgatcggatg
    10261 ctcgcgtgcg cctcgcgcat cggcattgcg ggcaccacga cgcgcgacgc ctttcaggcc
    10321 aatcgcgacg cgatgtcgtc gctctgtcca ctggggtttg tcgaggatcg gttgggggtg
    10381 aacgatcggc tctcggatcc tcgcggcctg aaacggctcc gagtcaaccc ggactggtcc
    10441 gtcggggaca ttatccagcg cctccgcgcg gcgttggacg acccgccatt cggttcctcg
    10501 gacaagcacc gtgcgcctcc cgcatgggtc gccgacgtcg gggaagtcag cgtggaacat
    10561 ggcgatctgg tgatgatcgg atcgcctcgg gtcgatctgt ggttgccggg cagccagtgg
    10621 ccattggact gggtgatcga ggccgacctg tggagtaatg gggcggacgt gtggcttgtc
    10681 caggagtcgg acacgcctgg gaagtcctgg cgctggggcg gaaacgaccg cgcgattgtt
    10741 ctccagcgaa gtcaatgggg acgcccaccc gagacggtgg ccacatttcc tagggggatc
    10801 accagcggcg tctggcacca cgtgaagttg gtcaagcgcg gcgccggtgt gtgggtggag
    10861 tgggatggac gccccttgag gaagcgtccc gtgtacctgt ccggaatggg gagaggcgtg
    10921 gtcggggtcg tcgggtggcg aagcgagggc ggggcaaccg ttcgaatgcg acacctcacg
    10981 ttttcccgtt accccttcga gatgcgatcg gtcagcgcga acccctcgga acacgaagtg
    11041 acggcattga tccgcgatgc accgaccatt gccgcgctgt cacccttggg cggagtgatg
    11101 tccggagatc gggtacagga cgtgcgcttc gaccgggagc tgctcgcgat cctgtctcac
    11161 cgctacgggt gggaagtggt ccccatgatc cgcgtgctgc ctggccggga ggccgccgcc
    11221 tttggtgcgt ccgagggcgc gccgagcagg ccgtcggtcc aatggtccgc cacgctgctt
    11281 gactatatcg aacggaatgg ttggagcggg attggtctcg atctgcggca tcttccccct
    11341 tcggctcgtc cggaactcgg cgacggtatt gaccaacttg atcgacggct ccgcgcccag
    11401 gggcgacgat tcgtccagac caccgggggg gacaaacgtc tccacgcgtc gccagcctat
    11461 gccagccgtt gacccgccaa agcgctccgt ctatcgcgta ttggtcacgc tgggattgat
    11521 cggtacggtg gcgttgctga cgttcgcatc gccggcgtgg gcggcctctc ccggccaaaa
    11581 ggccctggac aagggcgtgg agctgtatcg gaacgggcga ttcaccgcgg cggccgacgc
    11641 gttccgagag gcgattcaac gcgatccggg gctgctcaag gcgcgggaga acctgggctg
    11701 ggcatactgg agggcaggcc gtttggcgga gtgccggaag gtgtggacgc agtacgccgc
    11761 gctgtatccc gaccgacccg agccgttcaa tctcttaacc cagcttcatc ttgcccaaaa
    11821 gaaatacccg gctgcgatcc aaaccatcaa gaagagtttg gccctcgcgc cggatcaacc
    11881 gaaagaacgg ctcaagctgg ccaaggcgct gctctgggac cgtcaattcg atcaagcgaa
    11941 acgctacgcc gcgctccacg cgaccgcgta tccggatgat gtcgccattc aagtgttctg
    12001 gggagaagtg ctcatgcgat atcacgattt tcaagcggcc gaggcgcagt ggcgcaaggt
    12061 gctggcgctc ggtcctccct cacccagggt gcggtattac tggatccgag cgctctacaa
    12121 cctgggacag tacgacgccg ccctgctcga agcccggaag gcgatcgcga cggacggtcc
    12181 gcagcaggac ctgctgcaac tgttgatcga ggacgctttg gcgcgagacg acgcggccga
    12241 ggccgtgcgt tggtatgaac tcgcggtcga acaattcccc gagcgccccg cgttctgggt
    12301 cgagttggcg gagttgtatc gcgagcgcgg gatgatgagg gccagcgacg cgacgctccg
    12361 tcgcgcgaag cagaggcacc cggagcactt cgaaatcttc atcaacgtag ccgacaacca
    12421 ccgcctgggg aagcggtacg acaaggctct tcgcgagtac cgggaactct tcgcgcggta
    12481 cccgcagaat cgcgatgcgt tcatcggcct cttcaacacc ctggtcgaag ccaaccacgg
    12541 gcacgaggcg ctcgacctgc tcgagcacaa tcagacgacg tttctgaccg attccgagct
    12601 ggacatggac agagcgcgcg tgctgctcgc gcaaggacag acctcccgcg ctcaggcgct
    12661 gttcatgcgc gtggcgtcgc cggagaagca gtacgtcccc attgtgctgt atcacggatt
    12721 gggcgggcac ccgcggagcg cgaacttggc ggttgcgctg ttcgattccc aactcaaggc
    12781 cttgcgcgac gcgggctaca ccgccatcac ggttcgcgaa ctggcggaga tgatcgacgg
    12841 tcgacagccg tttccgcgcc agccgatcgt catcaccttc gacgatggcc gcttggactc
    12901 ctttcaactc ggcgaccccg tgttggcgaa atacgggatg aaagcgacca tgttcgtgcc
    12961 cagcgcgcac atgattgacc ggcacccatt ttttgccgat tgggatctga tccgacggta
    13021 cgctcgtacc ggccggtggg atatgcaggg ccacggccac gaggcccatg atccgattcc
    13081 catcgacgag tccgggcaga ccggaggatt tctcgtcaat cgacagtggc tggaagccga
    13141 ccagcggctg gagaccccag aagagtacgc tgagcgcctg gaggctgact accaggagac
    13201 gatccgtctc ctgacacgaa acgttccaaa cctcgaggtc gtcgcctacg cctttcccta
    13261 ctcagaggcg ggccagtcga gcgtcggcaa cgcatctgac gccgcaaccc tcaatgagcg
    13321 gttgctcttt cgctattttc gctacggctt cgtgcaggac tccatcggct ataacgaggt
    13381 gaatcccacg gtgccgacca tgctgagacg gttgaacgtc ccgcgctcct gggacggcga
    13441 gagactcctc gcgcacttgg caacccagca tccacgccac aaggcccaga tgcacatcgg
    13501 tacgtcgtgg atgtgggccg ggcagtatgg caaatcgcgg cgggtgttcg aggccctggc
    13561 tgcggaaaac gttctgctca aaggcccgag cgaataccag cttgctgaag tggcctttca
    13621 ggaggggcag ttccgtgaag cccggcgaca tctggaaacc gccatcgagt acggtgagcg
    13681 atccagtcgg ggcgagcaac tgatggcgcg tctgcggtgg gaaaaccagc cgcgcgttga
    13741 tgcccgattc ggcacctccc atgattccga tgaacgggtc acctggtgga acacgctacg
    13801 ggtgatgtat cccttgacga accccgtcac gctgtggacc gacgtggggg tgattcgcct
    13861 gaccgaggac ggtttgcccg ccctctcggg ccgtgaggtc ggcgtgggca cggagtggcg
    13921 cggaacgggt ggcctgggcc ttcgtgctca gcttcgccat cgatcgattg agaagagccc
    13981 gaacagtctg aacgtgtggc tcgatggcat gtacgagcgg ggtccccacg agctcagatt
    14041 gcgatgggcg tccgaagacg ttgacacggt tcaggcccat gagaagggcc tgcaaagtcg
    14101 cgggtacacg gcggcctatc gccttcgacc gacaccgaat tgggacggac gaatccaggc
    14161 gtcgatccgc tcctacgacg atgggaatga tcgggtcgac tggcgcaccg cgatcacgcg
    14221 ccgcctccca acgcaggcgc aatggcgcgt ggggggcgcg ctctcgtaca gcgacaccgg
    14281 ggtgcagtcc ctgcgatatt acactccgga agacttgatc gtcggacgcg cgatcgtggc
    14341 ctaccgccaa cggtgggagt cgggatggga gctggcgggc gaagccgggg tggggtgggc
    14401 ggacgatgcg ctccgcggca atcgcatggt cagcgatgtc tcgctcgacg ctctccaggt
    14461 gtggggcgag cggctgcaat ctcaactggg atgggcttac gccaggaacc cggggtatca
    14521 aagctggtgg ctggaggggt cggtgtcgct tcgattctaa cggccgcggg attcctcggc
    14581 cgcgaacggg ggcaggagcg ccggatcctg gcgtcccagg ctccacagca gcacggtccg
    14641 atacccaagg gaccggatca cctccagctt ttccgtcagg ctgaccgcgt cctcgtagta
    14701 cacagtgtgc ggctgctcgt tcacccgata ggtgaaatac ggaacctgat cgtccgcgtc
    14761 gcgcgagatc gacgcgccgt gctcctgcgc cagcgcgatg gcttgatcgt actggatacc
    14821 ctcgaccgtg ccgtccgccc agcgcatccc gctcactttc aacaccggga ccacgcgctt
    14881 gggatcgcac tgcgagttcg cgtagttcag aacgcccctg atccatcgtc cggtggccat
    14941 gggccccggg ccggtggctc ggctgtgcag gttgtacaac ataatctgga gccggtccgc
    15001 ggtcgcgcag agcggccccc agtccatcgc gccggagccg tcggaccagc ggtccccgag
    15061 cttgggttgc accgtcacgg acagcaagac gccctgccgt cgcgcgtcgg cggccaattc
    15121 acggacgaac gtcgtgaaca aggctcgatc gcgggcccgg aggttctcgt agtcgatatc
    15181 cacgcccgac atgccgtatc gcgaggccag gtccatgatc gcggcgcgat gcgccgctcg
    15241 tcgttcttcg ccgctgagga ggtcgtgcac aacctgcgga tccttcagcg cgatgccgtg
    15301 gcccgacgcc acgtcgttga cgatggtgag ccagggccgc gccccggcgg ccctgacgcg
    15361 cgcgaccgcg gactccacgg gcaggt
//
LOCUS       DBNGTT010000130         4691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1303805, whole genome shotgun sequence.
ACCESSION   DBNGTT010000130 DBNGTT010000000
VERSION     DBNGTT010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1303805"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC16BC_03015"
     CDS             complement(<1..209)
                     /locus_tag="AC16BC_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3240618.1"
                     /translation="MIHTTIVFLIVLGFLIFVHELGHFLVARWTGVRVLRFSLGFGPK
                     IFSKQVGDTEYMVSAIPLGGYVKMAG"
     gene            complement(206..1417)
                     /locus_tag="AC16BC_03020"
     CDS             complement(206..1417)
                     /locus_tag="AC16BC_03020"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR3240619.1"
                     /translation="MPRTRVIILGATGSIGRSALDVAAAFPEEFEVTALAAGRGGDAF
                     ERQIRAVRPRVASVASAEEAARLRVRCADLPTEILGGPDAAATVAQCVDGDVVVSAIV
                     GAAGLLPTVAAIRAGKTVALANKETMVMAGALVTAEAARRGTEILPVDSEHSAIFQVL
                     AGHRREDVRRLILTASGGPLRDLPSDRLARVTPEDALRHPNWSMGPKITIDSATLMNK
                     GLEVIEAHWLFGVPAERIDVVIHRQSIVHSMVEFCDGAILAQMGVADMRGPIAYAMSH
                     PRRLPLPVAPLDVLGLGALTFEAPDTARFPCLGYAYAALRAGGTMPAALNAANEEAVA
                     AFLDGRIGFLRIAETVDRVLHETSTGPTATVDDVLEADAAARRSARRFLGSNPTEAPV
                     LGNGSLREVRA"
     gene            complement(1425..2282)
                     /locus_tag="AC16BC_03025"
     CDS             complement(1425..2282)
                     /locus_tag="AC16BC_03025"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3240620.1"
                     /translation="MKEHAGRIVTALGALPLLYAVIVWSPAPLFAVVIGLVTALAQVE
                     LYGMALPAGAARTWGGGRLQVFAVGGLAGVALLGLIAWSPASALPPAMVTVAVLGIAV
                     TALMFGRDLRAAGADAAFALFGVWYVAWLLGYVVRLRALPDGAWLVVFALWVTWIADA
                     AAYYVGKTWGRTPLAPRVSPKKTVEGAVGALAGGVLASVAAAAWFVGAFSWGEAAVLG
                     GIVAVAGMVGDLFESLIKRGAGVKDSGGLIPSHGGMLDKIDGLLFTAPLIYHYMAWVK
                     RAGASAAAP"
     gene            complement(2279..3064)
                     /locus_tag="AC16BC_03030"
     CDS             complement(2279..3064)
                     /locus_tag="AC16BC_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR3240621.1"
                     /translation="MPTSVVGHESDEELQARVLRGPLPRHVAIIMDGNGRWAASRSLP
                     RVAGHREGIKSVRETVTACRELGIAGLTIYAFSAENWRRPAFEVRELMMLLELYLQRE
                     LNTLLEKDIRFRTIGRIERLPATVVDWIRRVERETAGCSEMTLTLALSYSGRSEIVDA
                     ARRMVADARAGALDPSSIDEDTFARYLDTREFEDPDLVIRTSGECRISNFLLWQIAYA
                     ELYFTKTLWPDFRRRDLLTALLDYQRRERRFGRTGEQLQGSPG"
     gene            3706..4644
                     /gene="sppA"
                     /locus_tag="AC16BC_03035"
     CDS             3706..4644
                     /gene="sppA"
                     /locus_tag="AC16BC_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3240622.1"
                     /translation="MRSPLSLLACALISLLAGGCVTVNVRPATQPLQEVRLSGEGRDK
                     LLLIEITGFLSGEPRGGKILGEETPSLVSEVTEIVRKAERDPRIKGVILRINSPGGTV
                     TASDTIYHELLRYKQRARIPMVAHILDLGTSGAYYIAQAADRIVAQPTTVTGSIGVIM
                     VRPSVSGLLEKIGVETAEITSGSHKAMGSPFRPLQDGDRALFQSVIDDLYGRFVDAVA
                     AGRPKLARERIVELADGRIYTAEQAKSAGLVDGLGYLDDTIATLRDAVGLTSATVVTY
                     SRPGEYKGSIYAAPTVNIDLKLWPTQHAGFLYLWQP"
BASE COUNT          845 a         1689 c         1466 g          691 t
ORIGIN      
        1 cccgccatct tcacgtatcc gcccagcggg atcgcggaga ccatgtactc ggtgtccccg
       61 acctgcttgc tgaaaatctt gggaccgaac cccaacgaaa agcgcaacac gcggactccg
      121 gtccagcggg cgaccaggaa atgcccgagt tcgtggacga aaatcaagaa tcccagaacg
      181 atcagaaaga cgatggtggt atggatcatg ccctcacctc acggagactc ccattgccca
      241 ggaccggcgc ctcggtcggg ttcgacccga ggaagcggcg cgccgaacgc cgcgcggccg
      301 catcggcctc caacacgtcg tcgaccgtcg cggtcggtcc ggttgaggtc tcatgtaaca
      361 cgcgatcaac cgtctcggca atccgaagga agccgatccg tccgtccaga aaggccgcga
      421 ccgcctcttc attggccgcg ttgagcgcgg cgggcatggt ccccccggcg cgcagcgcgg
      481 cgtaggcgta ccccaggcac gggaatcgcg cggtatccgg ggcttcgaac gtcagcgcgc
      541 cgagcccgag cacgtcgagc ggcgccaccg gcagcggcag ccggcgggga tgactcatcg
      601 cgtacgcgat ggggccgcgc atgtccgcca cgcccatctg cgcgaggatc gcgccgtcgc
      661 agaattcgac catggagtgc acgatgctct gccgatggat gaccacgtcg atccgctcgg
      721 ccggcacacc gaacagccag tgcgcttcga tgacttccaa ccccttgttc atgagcgtcg
      781 cagagtcgat cgtgatcttc gggcccatcg accaattggg gtggcgcagc gcgtcctcgg
      841 gcgtcacgcg ggcgagccgg tcgctcggca gatcccgcaa cggcccgccc gacgcggtca
      901 gaatcaggcg ccgcacgtcc tcgcggcgat gacccgccag cacttggaaa atggcgctgt
      961 gttcgctgtc cacgggcagg atctccgtcc cgcggcgcgc ggcctcggcc gtcaccagcg
     1021 cccccgccat caccatggtt tctttgttgg ccaacgccac cgtcttgccc gcgcggatcg
     1081 cggccaccgt cgggagcagc cccgccgcgc ccacgatcgc ggacaccacg acgtcgccgt
     1141 ccacgcactg ggccacggtg gccgccgcgt ccggcccacc caggatctcg gtgggaagat
     1201 ccgcgcagcg cacgcgcaac cgcgcggcct cctcggccga cgccaccgac gccacccttg
     1261 gccggacggc gcggatctgc cgctcgaacg cgtcgccgcc ccgtccggcc gccagcgccg
     1321 tcacctcgaa ttcctcggga aacgcggccg ccacgtcgag cgcggagcgg ccgatcgatc
     1381 cggtcgcgcc cagaatgatt acgcgcgtgc gaggcatcgc ttcctcaggg cgcggccgcg
     1441 gatgcgcccg ccctcttgac ccacgccatg taatggtaga tcagcggcgc cgtgaacagg
     1501 agcccgtcga tcttgtcgag catgccgccg tgcgacggga tgaggccgcc ggaatccttg
     1561 accccggctc cgcgtttgat cagcgactcg aacaggtcgc cgaccatgcc cgcgaccgcc
     1621 acgatccccc ccagcaccgc ggcctcgccc caggagaacg cgcccacgaa ccaggcggcc
     1681 gcggccacgg atgccagcac cccgccggcc agcgcgccca cggcgccctc cacggttttc
     1741 ttggggctca cgcgcggcgc gagcggtgtc cgaccccacg tcttacccac gtagtacgcg
     1801 gcggcgtccg cgatccaggt cacccacaac gcgaacacca ccaaccacgc cccgtccggc
     1861 agcgcccgca atcgcacgac atacccgagc agccaggcga cgtaccatac cccgaacagg
     1921 gcgaacgcgg cgtccgcgcc ggccgcccgg aggtctcgcc cgaacatcag ggccgtgacc
     1981 gcgatcccca gcaccgcgac cgtgaccatc gccggcggca gggcgctcgc cggggaccat
     2041 gcgatcaggc ccaagagggc cacgccggcg agcccgccga ccgcgaagac ctgcaggcgg
     2101 ccgccgcccc acgtccgggc ggccccggct ggaagggcca tgccatacaa ttccacttgg
     2161 gccaacgcgg tcacgaggcc gatgaccacg gcaaacagcg gagcgggaga ccaaacaata
     2221 acggcataca gaagcgggag ggcacccagg gcggtgacga tccgccccgc gtgttccttc
     2281 acccggggct cccctgaagt tgttccccgg tgcggccgaa gcgtcgctct cggcgctgat
     2341 agtccagcag cgccgtcaac agatcgcggc gacggaaatc cggccacaag gtcttggtaa
     2401 agtacagctc ggcgtacgca atctgccaca acaggaagtt gctgatgcgg cattcaccgc
     2461 tggtgcggat cacgaggtcg gggtcttcga attcccgggt gtccaggtac cgagcaaacg
     2521 tgtcttcgtc gatcgacgag ggatccagtg cgccggccct ggcatccgcg accattcgcc
     2581 gggcggcatc gacgatttcg ctccgcccgc tgtagctgag cgccaacgtc aaggtcattt
     2641 cgctgcaccc ggcggtttcg cgttcgaccc gacgaatcca atccaccacg gtcgcgggaa
     2701 ggcgttcgat ccgcccgatg gtccggaacc ggatgtcttt ctccagcagc gtgttcaact
     2761 cccgctggag gtacaactcc aacaacatca tcagctcgcg gacttcaaag gccggacggc
     2821 gccagttttc cgcggaaaac gcgtagatag tcaggccggc gatgccaagt tcgcggcacg
     2881 cggtgacggt ctcgcggacc gatttgatgc cctcccgatg gcccgcgacg cgcggaaggc
     2941 tccgcgaggc ggcccaacgc ccgttgccat ccataataat ggccacgtgc cggggcagcg
     3001 gaccgcgcag gactcgcgct tgtaattctt cgtcggattc gtgcccgacc actgaagtgg
     3061 gcatcgtctc taggtaaaaa tcatgaacgg cggtagtgta acgtgcgcca aaagtgaagt
     3121 caaacttgcc ggggcaccac cacgtggtgc cccttgtcaa tgcgccgcag gcaaccgttc
     3181 aagtggcgca gcaacccggg gcgagccagg ggctcgcaat ggaaaccggt atcatcaccc
     3241 tcctctcggg tggcgcggca acgcgcagca ggccgcgaag cagcctccga tggccatagc
     3301 gccgccaggc gcccgatcgc gtggcgcagc aacccgcagt gcgccgcggg cgcaccgtga
     3361 aaaaggtcat tttctcgaca catagccaag cggcgcgcga tggccatgac gcccacggcg
     3421 tccagttgac acgggtttcc cgggcgctat actctcggca ccatttgaca ccccggatcg
     3481 cccatgaaga gccctgagat ctctcgccag accgcccccg tcaacgtcga acgcggattg
     3541 ggttttgtcc tgctcgcgct cagccttgtt ggattgctga tcaacttcct ccagtaagtc
     3601 gtcattcctt accccgccgg cgccctacgt accgcgggcg accgcgtacc cgttgcacgg
     3661 gcggacccga cacggtagaa tgccggtccg gcctctgaca tgatgatgcg atccccactc
     3721 tcgcttctcg cgtgcgccct gatcagcctg ctggcgggcg gctgcgttac ggtcaacgtc
     3781 cgccccgcga cccaaccgct ccaggaagtt cgcctcagcg gcgaaggccg cgacaagctg
     3841 ttgctcatcg agatcacggg gtttctctcc ggagagccgc ggggaggcaa gatcctgggc
     3901 gaggaaaccc cgagcctggt gtcggaagtc acggagatcg tccgcaaggc cgagcgtgac
     3961 ccgcgcatca aaggcgtgat ccttcgcatc aacagccccg gcggcacggt caccgcgtcg
     4021 gacaccatct accacgagtt gctgcgctac aagcaacgcg cgcgcatccc catggtcgcc
     4081 cacatcctgg atctgggcac gtccggggcg tattacatcg cgcaggccgc ggaccgcatc
     4141 gtggcccagc ccaccaccgt gaccggcagc atcggcgtca tcatggtccg gccgtccgtg
     4201 tccgggctgc tcgaaaaaat cggcgtggaa accgcggaga tcacctccgg atcgcacaag
     4261 gccatgggct cgccgtttcg cccgctccag gacggcgatc gcgccttgtt tcagtccgtg
     4321 atcgacgacc tgtacggacg gttcgtggac gcggtggccg cgggccggcc caaactcgcg
     4381 cgcgagcgga tcgttgaatt ggccgacgga cggatctaca cggccgagca ggccaagtcc
     4441 gccggcctgg tggacggcct cggatacctc gacgacacga tcgccacgct gcgggacgcg
     4501 gtcgggctga catccgcgac ggtggtgacg tattcgcgac ccggggaata caaagggagc
     4561 atctatgccg cgcccacggt caacatcgac ctgaaactct ggccgaccca gcacgcggga
     4621 tttctgtatc tctggcaacc gtgatggacg accgcgagca cgcccatcaa cccgttgcgc
     4681 cggaatccgg c
//
LOCUS       DBNGTT010000131         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_921618, whole genome shotgun sequence.
ACCESSION   DBNGTT010000131 DBNGTT010000000
VERSION     DBNGTT010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_921618"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..633
                     /locus_tag="AC16BC_03040"
     CDS             229..633
                     /locus_tag="AC16BC_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR3240623.1"
                     /translation="MWEPWLLFRKPLEGRVQDNLRKWRTGGFRRPSSEKPFGDVVPSS
                     PTHKSERSLAPHPSLKPQGFLRVVVRSVLPLGEGVVLDPFAGSGSTLAAAEAVGYQSI
                     GVEKDSHYFDMARKAIPKLTMFKNGAHYGFTL"
     gene            complement(692..1222)
                     /locus_tag="AC16BC_03045"
     CDS             complement(692..1222)
                     /locus_tag="AC16BC_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240624.1"
                     /translation="MAIVSNTVDSTKFNRGATLPFELRLKDFEIAMQDIYDFFYDVNR
                     GLVDRGLERLDDMLRPAIMSGLLSDMLTASVAKHSRSLTENLYHNGHPDLIVKGVYPG
                     NSKNQVPMGLKSRRLENPAGPSIRMALAISGCVFLCTMSMLLRSPHVIVLQWLSRRST
                     SRRLHGRIFGRIFEAN"
     gene            complement(1292..1903)
                     /gene="tpx"
                     /locus_tag="AC16BC_03050"
     CDS             complement(1292..1903)
                     /gene="tpx"
                     /locus_tag="AC16BC_03050"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HIR3240625.1"
                     /translation="MKRWAMFVSALTILTACASPKPPAMTVATTSGTPGDGQTVTFKG
                     DALALEGTAIKVGDTLPSAVVTSNDLKPVNLAETKGKVRIISLVPSIDTPVCEEQTHE
                     LSEKNGGLDKQVELITVSMDLPFAQKRFAKEAKINNVTFLSDYKSAEFAKNTGLLIQP
                     LHLLARTVLVVDKDNVIRYMQVVPEITNLPDMQAAMKAAKGLV"
     gene            complement(2006..>3178)
                     /gene="purT"
                     /locus_tag="AC16BC_03055"
     CDS             complement(2006..>3178)
                     /gene="purT"
                     /locus_tag="AC16BC_03055"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HIR3240626.1"
                     /translation="SATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQLAH
                     RSHVINMADPAQLRVVIEKERPRLVVPEIEAIATDALVDIEHEGWTEIIPTARATRLT
                     MNREGIRRLAAEELKLPTSRYAFAESRDALQHAIDSGIGYPCVVKPVMSSSGKGQSVI
                     KTPADVDAAWTYAMTGTRVSLGKVIVEAFIDFDFEITQLTVRAIGESGKVETFFCEPV
                     GHVQIKGDYVESWQPQAMTPAALAKSREIAKLVTDNLGGRGIFGVELFVKGDQVWFSE
                     VSPRPHDTGMVTMISQMQSEFELHARAILGLPVSPALRAPGASAVIYGGVEAKAIAFD
                     GLAEALRVPQTEIRLFGKPESYTKRRMGVALAIGKDTDEARQRAKLAASLVKPVVV"
BASE COUNT          587 a          950 c          936 g          705 t
ORIGIN      
        1 cggttctttt tcgaatgggg ccgctgcctc ttgccggtcc tcgtccctgg cgcaaatgtt
       61 gtcattgcga gcaatccact cctgtcatat attgttgcaa ccgccgtagc ccgagcaggc
      121 cttgaacgac gaggagagat cgtccgcctc gtcatgacta tgcgtggggg cgatcgcccg
      181 aaggcggctc acgaagaatt ttctcttgtc agcgtcatgc cgcgctcgat gtgggagccg
      241 tggctcttgt ttagaaaacc attagaggga agggttcagg ataacctacg gaagtggaga
      301 accgggggat tccggagacc gtcaagtgag aagccgtttg gagatgtagt cccctcgtct
      361 ccgacccata aatctgagcg ctcgctggct ccgcatccaa gtcttaaacc tcagggattt
      421 ctacgagtag tcgtacggtc agtgcttccc cttggagagg gtgtggtgct cgacccattt
      481 gccggttccg gatcgactct tgccgccgcg gaagccgtcg gatatcagag cattggagtc
      541 gaaaaggatt cacactattt cgatatggcc cgcaaagcaa taccgaagct gacgatgttt
      601 aagaatggtg cgcactatgg gttcactctg taaatccaat tccgccgcag tttgtccacc
      661 ccagctcggt gtaaagtcgt tgtgcgagtc cctaattcgc ttcgaagatt cttccgaaaa
      721 tcctcccgtg taacctgcgc gatgtagacc tccgtgaaag ccattggagc acgatcacgt
      781 gcgggctccg taacaacatc gacatcgtac acaaaaacac acatccactg atcgcgagcg
      841 ccatgcgtat cgacggcccc gccggatttt cgagtcgcct tgatttcaac cccatcggaa
      901 cctgattttt actgtttccc ggatacaccc ccttcacaat caagtccggg tggccattgt
      961 gatacagatt ctccgtaagg cttctggagt gtttggccac gctcgccgtg agcatatccg
     1021 acaaaagtcc tgacatgatt gccggacgga gcatgtcatc caatcgctcc agccctctat
     1081 ccacgagacc tcggttcacg tcataaaaga aatcgtagat atcctgcatg gcaatctcaa
     1141 aatccttcaa gcgcaactca aaaggcaatg ttgcgccacg gttgaacttg gtgctgtcga
     1201 cggtattgct aacaatggcc attctgacct ctcatcaaaa ccggaacggc aacaacagac
     1261 atatttttgg cgtacttcgc tcgatttaat ctcacaccag ccccttcgcc gccttcatcg
     1321 ccgcctgcat gtccggaagg ttcgtgatct ccggtaccac ctgcatgtac cgaatcacgt
     1381 tgtccttgtc caccaccagc acggtgcggg ccaggaggtg caggggttgg atcaggaggc
     1441 cggtgttctt cgcgaactcg gcggatttgt agtcggagag gaaggtcacg ttgttgatct
     1501 tggcttcttt ggcgaatcgt ttctgcgcga acggcaggtc catgctcacg gtgatgagtt
     1561 cgacctgctt gtccaaaccc ccgttctttt cggacagttc gtgggtctgc tcttcgcaca
     1621 cgggcgtgtc gatcgagggg acgaggctga tgatccggac cttgcctttg gtttcggcca
     1681 ggttcacggg tttgaggtcg ttgctggtga cgactgcgga cggcagggtg tcgccgactt
     1741 tgatggccgt gccttcgagc gccaaggcgt cgcccttgaa agtcacggtt tgaccgtcgc
     1801 cgggtgtgcc gctggtggtg gccacggtca tggccggggg tttgggtgag gcgcaggcgg
     1861 tcaagatcgt gagtgcgctg acgaacatgg cccatcgttt catggaagca cctccatgtg
     1921 tggttcgact ggctcaccac gaacggttcg ttgtctagaa ccgttcaccc tgagcattgt
     1981 cgaagggtcg cgatctccaa ccttgttaca ccactaccgg cttcaccagg ctcgccgcca
     2041 gcttcgcgcg ctgcctggcc tcatctgtat ccttgccgat cgccagcgcc acgcccatgc
     2101 gtcgcttggt gtacgactcg ggtttgccga acaggcggat ttcggtttgc ggcacgcgca
     2161 gggcctcggc caatccgtcg aacgcaatgg ccttggcttc aaccccgccg tagatcaccg
     2221 cgctcgcgcc gggcgcgcgc agcgcggggg agacgggcag gcccaggatc gcgcgggcgt
     2281 gcaactcgaa ctcgctttgc atttggctga tcatggtgac catgccggtg tcgtgcggac
     2341 gcgggctgac ttcggagaac cagacctgat cgcctttcac aaagagctcc acgccgaata
     2401 tgccgcggcc gcccaggttg tcggtcacga gcttggcgat ctcgcgcgac ttggccaagg
     2461 ccgctggggt catagcctgc ggctgccagc tctccacgta gtcgcccttg atctgcacgt
     2521 ggcccacggg ctcgcagaag aatgtttcga ccttgcccga ctcgccgatc gcgcgcacgg
     2581 tcagttgcgt gatctcgaag tcaaagtcga tgaacgcttc cacgatcacc ttgcccaggc
     2641 tcacccgcgt gccggtcatg gcgtaggtcc aggccgcgtc cacgtcggcg ggggttttga
     2701 tcacggactg gcccttgccc gatgacgaca tcacgggttt gaccacgcag gggtagccga
     2761 tgcccgagtc aatggcgtgc tgcaatgcgt cgcggctttc cgcgaacgcg tagcgcgagg
     2821 tgggcagttt gagttcttcg gcggccaggc gccggatgcc ttcgcggttc atggtcaggc
     2881 gcgtcgcgcg ggccgtgggg atgatctcgg tccagccctc gtgttcgatg tcgaccaacg
     2941 cgtcggtggc aatggcttcg atctcaggca ccaccagacg cggccgctcc ttctcgatca
     3001 ccacgcgcag ttgcgcgggg tcggccatgt tgatcacgtg ggagcggtgc gccagctggt
     3061 ggcctggggc gttggggtag cggtccacgg caatgacctc cacgcccaac cgttgcaacg
     3121 cgatgatgac ttccttgccc aactcgcccg cgcccaacaa catgacgcgc gtggccga
//
LOCUS       DBNGTT010000132         5360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2710231, whole genome shotgun sequence.
ACCESSION   DBNGTT010000132 DBNGTT010000000
VERSION     DBNGTT010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2710231"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="AC16BC_03060"
     CDS             <1..391
                     /locus_tag="AC16BC_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240627.1"
                     /translation="LLLSGLSSPVLHINIDIKPGNGDSAAPINPKAKGKIPVALLGSR
                     DFSVIDVNVSSLTFGHTGDEASLSRCGDREDVNDDGILDLVCHFENQLAAFSTTDEEA
                     VLKGRLDDGRLFEGRGLLKVVPAKAVY"
     gene            652..1275
                     /locus_tag="AC16BC_03065"
     CDS             652..1275
                     /locus_tag="AC16BC_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240628.1"
                     /translation="MAAVAMALCLLPACMSLRIPRAGEPVVVRQDEALVFGRIRMLDA
                     KSETIEYSPFWFNPWDQPFFAPGPRMTLELRRLYPSGKAFTYKAYPAPAIEKTGSFFW
                     ILPAGEYVLLGNPRLLGSERFTEGETGTLARFTVPASGGTIYLGTLIIDIVFDFEDVV
                     RAWRTDEAEYAISGSRVVDDREQELTQLRARFPAIPEPVITEQMRIE"
     gene            1317..1868
                     /locus_tag="AC16BC_03070"
     CDS             1317..1868
                     /locus_tag="AC16BC_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240629.1"
                     /translation="MRAITVFLLVGVLWHADPVLAKNAGDHTVDPDRTSEERDLSRLA
                     HVQADPANTIAEFTTDGCSGGLSDGWVTLSRVLPAFRSAFGDRPPYEFCCTTHDRAYW
                     RGETEHGYAKRLEADETLRRCVIDYGTQHRAEFAERFRLPEDTIVTNFQAIGDLMYLA
                     VRAGGKPCSSFPWRWGYGWPKCP"
     gene            1914..3635
                     /locus_tag="AC16BC_03075"
     CDS             1914..3635
                     /locus_tag="AC16BC_03075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240630.1"
                     /translation="MRLLFRVLTYLSPYRGLAAATFACAVLATLVDLAPPWIIKLVID
                     TVVRPGDVGPLPWLMLSLAGVYVARNLLSGARIWFNNRLEQSVIYDLRKAVYDKLQSL
                     SVRYYDQRATGELASRVTGDTNNLERIFIDGVESLLMAGLTLIGITIILFTMHWRLAL
                     LALVPIPFLILGASRFTRRIHGLYRIIRRESGQLNAVLQDQLSGIREILAFNREAHAS
                     SRFDTHNRAYAHEQLHAARVWSVYSPAMVFLGSAGTVLILWVGSREVVAGAMTLGELV
                     AFLSYLALFYVPINQIHSVNHMLQHALASGERVFEVLDAKPDVTDRPGAAALTRASGQ
                     IRFEAVSFGYRPDVPVLQDLEFTAESGETIALVGPSGAGKSTVIKLLLRYYEVGAGRI
                     LLDGRDIRDLTLASLREQIGIVAQDPFLFNGTVRENLLFGRLDATVAEVEAAAAAAHA
                     HEFIVAMPEGYDTLIGERGVKLSGGQKQRMAIARALLKDPPIIIFDEATSNVDSETEA
                     KIHDALERLIAGRTTLIIAHRLSTLRAVDRLIVLDHGRLVETGRHHDLLARGGLYAAL
                     YDAQARV"
     gene            3787..4731
                     /locus_tag="AC16BC_03080"
     CDS             3787..4731
                     /locus_tag="AC16BC_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240631.1"
                     /translation="MKVVMALATAAIILETAGASATQARSVPLIEEIPVHPAAAPAPR
                     DDSLSAPQLGFTLETERLYIVKASPEAVVKFYQSRLSAREVTEEQFDAAIDQAEAGAL
                     RGALFTFESHDLKSPALRGIKDAIQKRPPHRPGQWIAWARFVWSRRQSNDEVAQFGIG
                     IVDGLDMRRTEPQTVLLLAAGVPGGDADDTGEAEDEAATQQEHVAPQASAPEPSAAEL
                     GVPRYPGAVFDGLNSAGMSSDEERYFIYTTGDPQDKVVAFYERQTGKKALKTEGGAMI
                     PVEGTGLFPELGVTIQPNTGSYPRAVRTIVTVRNGRGQ"
BASE COUNT          948 a         1731 c         1703 g          978 t
ORIGIN      
        1 gctgctgctc tcgggtttga gctccccggt gctgcacatc aacatcgaca tcaagcccgg
       61 aaatggcgac tccgctgccc caatcaaccc taaggcaaaa ggcaagatac ccgttgcgct
      121 gcttggatcc cgggacttca gcgtgattga cgttaatgtt tcgtcactca cgttcggaca
      181 cactggcgac gaagccagcc tgagcagatg cggcgaccgt gaggacgtca acgacgacgg
      241 cattctggac ctggtctgcc acttcgagaa ccagctcgcg gcattctcaa caacagacga
      301 agaagccgtt ctcaagggac ggttggatga tggccgcttg tttgaagggc gcggtttgct
      361 aaaggtggta ccggcgaaag ccgtgtacta accgcatggg gtaaaaaggg ccccggcgtc
      421 cccagcgagc cattgcgcga tggggcgccg gggcctcttc tcttgtcaga caccactgga
      481 ctgcccccgt cttctggcat aatggggggc agagtacttt tgtcttgttg acaccacccc
      541 ccctggccca ctaacaagat gtgccgccga gcaaaagcac gttggcgaat gagctgggaa
      601 tgttccttgt ttgggtggga ccgtccccgg ccctatcccg ctgccgcgtt ggtggctgcg
      661 gtcgcaatgg cgctctgtct gctgcccgcg tgcatgtccc tccgcattcc ccgcgctggg
      721 gagcccgtcg tggtccggca ggacgaagcg cttgtctttg gccgcattcg catgctcgac
      781 gcgaagagcg agaccatcga gtattcgccg ttctggttca atccgtggga ccaaccgttc
      841 ttcgcaccgg ggccgcgcat gaccctggag ctgcgtcggc tgtatccatc cggcaaggcg
      901 ttcacataca aggcgtaccc ggcgcccgcg atcgagaaga ccggttcgtt cttctggatc
      961 ctgcccgccg gggaatacgt gttgctcggc aacccgcgat tgctcggatc agaacgattc
     1021 accgaggggg aaaccgggac actggcccgc ttcacggttc ccgcgtccgg cgggacgatc
     1081 tacctcggga cgttgatcat cgatatcgtc ttcgatttcg aggacgtggt ccgcgcctgg
     1141 agaacggacg aagcggagta cgccattagc ggctcccgcg tggtggatga tcgcgagcag
     1201 gaactgaccc agttgcgtgc gcgcttcccg gcgatcccgg aaccggtgat caccgaacag
     1261 atgcgcatcg aatgattcag ggcccgtaag ggacgttgcc tggtgatgag acccacatga
     1321 gagcgatcac ggtcttcctg ctggtcggtg tactctggca cgccgatccg gtcctggcca
     1381 agaacgcggg cgaccacacc gtggaccctg accgcacgtc ggaagagcgg gacctgtcgc
     1441 gtctggcgca cgtgcaggcc gacccggcca acacgatcgc ggagtttacg accgacggtt
     1501 gcagcggcgg cttatcggac gggtgggtga cgttgagccg agtattgccc gcgttccgga
     1561 gcgcgttcgg ggaccggccg ccgtacgagt tttgttgcac aacgcacgat cgcgcgtact
     1621 ggcgcggcga gaccgagcac gggtatgcca agcgcctgga ggccgacgaa accttgcgcc
     1681 gctgcgtgat tgattacggg actcaacatc gcgccgaatt cgccgaacgc tttcgtctgc
     1741 cggaagacac gatcgtcaca aactttcagg ccatcggcga cctgatgtat ctggccgtgc
     1801 gcgcgggcgg caagccgtgt tcgtccttcc cctggcgttg gggatacggt tggccgaaat
     1861 gtccctaagc ccccctaccg ccagaccgcg ccggaccatg gtataaccca acgatgcgcc
     1921 tcctcttccg tgttctgaca tacctctcgc cgtatcgcgg cctggccgct gccacgttcg
     1981 catgcgcggt gctcgccacg ctcgtggacc tggcgccgcc gtggatcatc aagctcgtga
     2041 tcgacaccgt ggtgcgcccg ggcgacgtgg gaccgttgcc ctggctgatg ctgtcgctgg
     2101 ccggggtcta tgtggcgcgc aacctcctgt ccggcgcgcg gatctggttc aacaaccggc
     2161 tcgaacagtc cgtgatctac gacctgcgca aggcggtcta cgacaagctc cagtccttgt
     2221 ccgtgcgcta ctacgatcag cgcgccacgg gcgagctggc gtcgcgggtc accggcgaca
     2281 ccaacaacct ggagcggatc ttcatcgacg gcgtggaatc gctgctgatg gccgggctga
     2341 cgctcatcgg catcaccatc attctgttca ccatgcactg gcggctggcc ctgctggccc
     2401 tggtcccgat tccgttcttg atcctgggcg cctcgcgctt cacccgccgc atccacggcc
     2461 tgtaccggat catccgccgc gagtcgggcc agctcaacgc cgttctgcag gatcagctct
     2521 ccgggatccg ggagatcctg gcgttcaacc gcgaggcgca cgcctcgtcg cgcttcgata
     2581 cgcacaaccg cgcatacgcc cacgagcagc ttcacgcggc gcgggtgtgg tcggtgtact
     2641 cgcccgccat ggtgttcctg ggttcggcgg gcacggtgtt gatcctgtgg gtcggctctc
     2701 gcgaggtggt ggccggggcc atgacattgg gcgaactcgt ggcgtttctg tcgtacctgg
     2761 cgttgttcta cgtgccgatc aaccagatcc actcggtcaa tcacatgctg caacacgcgc
     2821 tcgcgtccgg cgagcgcgtg ttcgaggtgc tggacgcgaa gcccgacgtg accgaccggc
     2881 ccggggccgc cgcgctcact cgcgcgtcgg gccagatccg gttcgaggcc gtgtccttcg
     2941 ggtaccggcc ggacgtcccg gtgttgcagg acctcgagtt cacggcggaa tccggtgaga
     3001 cgatcgcatt ggtggggccc agcggcgcgg gcaagagcac ggtgatcaaa ctgctcttgc
     3061 gctactacga ggtgggcgcg ggacgaatcc tgctcgacgg acgcgacatc cgggacctga
     3121 cgctcgcgag cctgcgcgag cagatcggga tcgtggccca ggacccattt ttgttcaacg
     3181 gcacggtgcg ggagaatctg ctgttcggcc ggctggacgc gaccgtggcc gaggtggaag
     3241 ccgcggcagc cgccgcgcac gcccacgagt tcatcgtcgc aatgcccgag ggctacgaca
     3301 ccttgatcgg cgaacgcggc gtgaagcttt ccggcggcca gaaacagcgg atggccattg
     3361 cccgcgcctt gctcaaggat cctccgatca tcatcttcga cgaagccacc tcgaacgtgg
     3421 acagcgagac cgaagctaag atccatgacg cgctggaacg cctgatcgcg ggccgcacca
     3481 cgctgatcat cgcccaccgc ctgtccacct tgcgcgcggt ggaccggctg atcgtcctgg
     3541 accacggccg gctcgtggaa accggccggc accacgacct gctcgcgcgc ggcggcctct
     3601 acgccgcgtt atacgacgcg caagcgcgcg tgtgacccga cgaaccagac tggtcgggag
     3661 aaccgatggg ccggtcacga gtgagaagat catctgtggc cgataaccgg tcgcggattg
     3721 atttcgagac cgacacgatc tatattcggc tatcggcaat gccgctcgtc gcgtgaaggg
     3781 agcgctatga aggttgtcat ggccctggcg actgctgcga tcatcctcga aaccgccggc
     3841 gcttccgcga cccaggcgcg gagcgtcccg ctcattgagg agattcccgt tcatcccgcg
     3901 gcggcgccgg ccccgaggga cgattcgctg tccgctccac agctcgggtt cacgctcgag
     3961 accgaacgcc tctacatcgt caaggcctct ccggaggccg tggtcaagtt ctaccagtcc
     4021 cgcctgtcag cgcgcgaggt caccgaggag cagttcgacg ccgccatcga tcaggcggag
     4081 gcgggtgcgc tccggggagc gctcttcacc ttcgaatcgc atgacctgaa atcgcccgcg
     4141 ctgcggggca tcaaggacgc gatccagaag cggccgcccc atcggccggg tcagtggatc
     4201 gcctgggcgc gctttgtctg gtcccgacgc caatccaacg acgaggtggc gcaattcggg
     4261 atcgggatcg tggacggctt ggacatgcgc cggaccgagc cgcagaccgt cctcctgctc
     4321 gccgccggcg tacccggtgg tgatgccgac gacacgggcg aggcggaaga cgaggctgcg
     4381 acgcagcaag agcacgtcgc gccccaggcg tccgcgccgg aaccgagcgc cgcagaactc
     4441 ggcgtgccgc gctacccggg ggccgtcttc gacggtctca actcggctgg aatgtcctcc
     4501 gatgaggagc gctatttcat ctacaccacg ggcgatccgc aggacaaagt ggtggcgttc
     4561 tatgagcgcc agactggaaa gaaggccctg aagacggaag gcggcgccat gattcccgtg
     4621 gagggcaccg gcctgtttcc tgaactgggg gtcacaatcc agcccaatac gggttcctat
     4681 ccccgggcag tgaggacgat agtcactgtg cgcaacggca ggggccaata gaaccgacca
     4741 tctccacccg tcgcacgccg cccaacggaa ccgatcgggg acgttcctac ctcattgagc
     4801 cagtaatttc tgattctgat ttcccagccc tccccgtcgt cccgctgcgc acggccatca
     4861 cggtatacat ctccgggtta cacgggctcc aatttcacgc cccttgatcg ccgtcgaagt
     4921 aggaacgtcc ccaaagcacg agagtgggtt atacttcaag gtcgctcgcc gagggaggcc
     4981 gacgtgagaa cttggcgtaa acacgtcacc actgcagacg gtgaacgatc ggtctccgag
     5041 cgcctcttcc gggatgggca atggtttcct tccacgaaac cgggaagaaa cgtgctcgcg
     5101 ccgacgctgt tttttgcttg cctgatcgcg tccgactgta ccgggtcacg cgaccgtaac
     5161 atggtgggca gcggtcgggg ccacggctca aacagcaatc cggttgtcag cgccgaacag
     5221 gtctccctcc aaaagaaaag caactgtctt gcgagtcaac acgtttttta ggcatgacta
     5281 gtccgccacg gggtgcgatg tttgagcatg gcgttgagga tcgtgaggag cttgcgcatg
     5341 caggccacca gggcgacctt
//
LOCUS       DBNGTT010000133         2431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2454019, whole genome shotgun sequence.
ACCESSION   DBNGTT010000133 DBNGTT010000000
VERSION     DBNGTT010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2454019"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16BC_03085"
     CDS             <1..375
                     /locus_tag="AC16BC_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017971995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HIR3240632.1"
                     /translation="KGSLFLCSLVLSASLMPVSSLPDPSWQTAFGLGFVSAVFDNIPL
                     TALALFQGGYDWGVLAFAVGYGGSMIWFGSSAGVAICNLFPEARNTGRWLAEGWHVAV
                     AYVIGFFALLAVFGWHPDILPR"
     gene            558..1079
                     /locus_tag="AC16BC_03090"
     CDS             558..1079
                     /locus_tag="AC16BC_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240633.1"
                     /translation="MAQVTRKDLVRHERWGVGRVEAVFGVDTSARCGVYFTRTDQQVV
                     VPMGELERLSPTESAVYDLAKLALYDLRAEDAPDTELADRWKGGELVLKPAKAELEAK
                     IVPLDAFFHKIVMARDRLRVLEQHVNAHKGLSDADKVQLQQYITRIYGSLTTYNVLFK
                     NRDDQFVGQKGEE"
     gene            1120..1842
                     /locus_tag="AC16BC_03095"
     CDS             1120..1842
                     /locus_tag="AC16BC_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR3240634.1"
                     /translation="MTAANGFTRYQKFLLATFVVLWVALAIAPWYRQDWLLENILVAV
                     LVPTGLVIVRYFRLSDLSYTLITLFLILHITGAHYTYEKVPFGETLSAWFGASRNHYD
                     RVVHFSFGFLLAYPIREMFLRIASVRGFWGYVLPLDVTLSCSAIFEIFEWLVAAVVNP
                     QAGLAYLGSQGDMWDAQKDMALAGLGALITMTIIALVNWRYDDSFWQEWRDSLRVKSH
                     MPLGEVRLRELIKAREQQRRKG"
     gene            1913..2107
                     /locus_tag="AC16BC_03100"
     CDS             1913..2107
                     /locus_tag="AC16BC_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240635.1"
                     /translation="MAYKRTIVPVEHIESKILVIRTQKAMLDIDRAALYDAQFKIVFD
                     AIRELMTSPKTERRRIGFEV"
BASE COUNT          444 a          738 c          777 g          472 t
ORIGIN      
        1 aaaggcagct tgttcctgtg ttcgttggtg ttgtccgcga gcctgatgcc ggtgagttca
       61 ttgcccgacc cgtcgtggca gaccgcgttc gggctggggt tcgtgagcgc ggtgttcgac
      121 aacattccgt tgaccgcctt ggccctgttt caaggcgggt acgattgggg cgtgctcgcg
      181 ttcgcggtgg ggtacggcgg ttccatgatc tggttcgggt cgtccgcggg cgtggccatt
      241 tgcaacctgt tccccgaggc ccgcaacacc gggcgctggt tggccgaggg ctggcacgtg
      301 gctgtggcgt acgtcatcgg gttctttgcg ttgctggcgg tgtttgggtg gcatccggac
      361 attttgcccc gctaggggga gcctgtccat gaagggccat ctgcggcgtt gctgctgcgc
      421 ttccggtcct cacgtacgac ccagtacgct ccggtccggt tctcgcagcg ccttgcatct
      481 gacccttcct gaacaggctc cgggtggaga tccgggacaa cggacatcca aaagtgcatg
      541 cgataaggga ggagtcaatg gctcaggtga cgcgtaaaga tctggtacgg catgaacgct
      601 ggggggtggg gcgggtggag gcggtgttcg gggtggatac gagcgcgcgc tgcggggtgt
      661 acttcacgcg gaccgatcaa caggtcgtgg taccgatggg cgaactggaa cggctctcgc
      721 ccaccgaaag cgcggtgtac gatctggcca aactcgcgtt gtacgacctg cgcgccgagg
      781 acgcgccgga caccgaactc gcggatcgct ggaaaggcgg ggaactggtt ctcaagccgg
      841 ccaaggccga attggaggcc aagatcgttc cgctggacgc gttctttcac aagatcgtca
      901 tggcccgtga ccggttgcgc gtgctggagc aacacgtcaa cgcgcacaag ggcttgtcgg
      961 acgcggacaa ggtccagttg cagcagtaca tcacccgcat ctacggctcg ctcaccacct
     1021 acaacgtgct gttcaagaac cgcgacgatc agttcgtggg gcagaagggc gaggagtaga
     1081 tcctccccac ccacacctgg cgccggtact ggagggccca tgaccgcagc caacggcttc
     1141 acgcggtatc agaaatttct gctggccacc ttcgtcgtcc tctgggtggc gcttgccatc
     1201 gcgccctggt accgacagga ctggctgctg gaaaacatcc tggtggcggt tctggtcccc
     1261 accgggctgg tgatcgtgcg ctacttccgc ctgtccgacc tttcctacac cctgatcacc
     1321 ctgttcctca ttctccatat caccggcgcg cactacacgt acgagaaagt cccgttcggc
     1381 gaaacgctca gcgcatggtt cggcgcgtcg cgcaaccact acgaccgggt cgtgcatttc
     1441 tcgttcggat tcctgctcgc gtatccgatc cgcgaaatgt tcctgcgcat cgcgtcggtg
     1501 cgcggcttct ggggctatgt gcttccgctc gacgtcacgc tctcgtgcag cgccatcttc
     1561 gagatcttcg agtggctggt ggccgccgtg gtcaatcccc aggccggcct cgcctacttg
     1621 ggctcgcaag gggacatgtg ggacgcccag aaagacatgg ccttggcggg cctgggcgcg
     1681 ctgatcacca tgaccatcat cgcgttggtg aattggcgat acgacgactc gttctggcag
     1741 gaatggcgcg acagcctgcg cgtcaagagc cacatgccac tgggcgaagt gcggctgcgc
     1801 gaattgatca aagcgcgcga gcaacaacgg cgcaagggct gaggcgagcg gctgccccgg
     1861 acgtaagccc gaagatccgg ggcgagcgca gctacggtgg agggccttgg caatggcgta
     1921 caagagaacg atcgtcccgg ttgagcacat cgaatcgaaa attctggtca ttcgaacaca
     1981 aaaggccatg ctcgatattg accgcgctgc gttgtacgac gcccagttca aaatcgtctt
     2041 cgacgccatc cgcgaactca tgacctcgcc caagaccgag cgccgacgaa tcggattcga
     2101 ggtctgaaaa cgccgaccgc gctacgggcg atacgcatcg gctaaccgcg gggtgtgaca
     2161 ctcggcatgg ctcttctaaa gggtcgtcac gggtcaagat ttggcccact cacgttgttc
     2221 gagaggcaac cacggtccat cgagcgccta tagatttcgc agaccaaatt gggtaggctc
     2281 accgcactgg cggccggacc aaacacgatg ggcgacctac ctgaaaagct cgacctccgt
     2341 tcccaccaca tcgccgacga caagcggcag gagctgctgc gcctgtttcc ggaaatccgc
     2401 accgagggcg gcaagctcga cttcgaacgg c
//
LOCUS       DBNGTT010000134         3875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1816163, whole genome shotgun sequence.
ACCESSION   DBNGTT010000134 DBNGTT010000000
VERSION     DBNGTT010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1816163"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="AC16BC_03105"
     CDS             <1..300
                     /locus_tag="AC16BC_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HIR3240636.1"
                     /translation="LAAKLSPEKYQLFTEVMGGARSECKQIRQQIRASREHALGILTA
                     PEFDEASFQREVDTLHELRGRVADRLAGATLELAKQFDPEERKLLGKYLERPPPP"
     gene            399..1616
                     /locus_tag="AC16BC_03110"
     CDS             399..1616
                     /locus_tag="AC16BC_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3240637.1"
                     /translation="MRQGRFASVKWVVVVAVGLVVGGGLWYWMARNDAAAEYQTAVVT
                     RGALTQVVTATGQLNPVVSVQVGSQISGIIQKLFVDFNSRVKAGQVIAQLDSSTFVAN
                     LRQAEGELANARAGLELARVKATRAEELRAKDLIPQAEHDQAQADLHQAEAAVVIREA
                     AVRKAAVDLERCTIEAPIDGVVISRSVDVGQTVAASLSAPTLFVIANDLTKMQINASV
                     AEADIGGVEVGQDVNFTVDAFPSQRFHGRVVQVRNAPVTVQNVVTYDTIIEVANPELR
                     LKPGMTANVSIVVANREGVLTVPNAALRFRPPDAATSVAPSTAGGGTNATTKTRGGTS
                     AGRAVYLAPQGKDRPAPERVPVKTGITDGVVTEVLEGLQEGDAVVIGVRFDQSNSDSR
                     SGGSNPFGGPRRF"
     gene            1633..2382
                     /locus_tag="AC16BC_03115"
     CDS             1633..2382
                     /locus_tag="AC16BC_03115"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240638.1"
                     /translation="MSGLIVLEHVSKTYRSGDIDVHAVRDVSAEIARGEFVAVMGASG
                     SGKSTLMNIVGCLDRPSSGRYVLDGVDVSGLDGDQLADLRNAKLGFVFQGFHLLPRTS
                     ALENVELPMLYARGVNRAQQRERAMRALEIVGLTARADHHPNQLSGGQQQRVAIARSL
                     VNEPVVLLADEPTGNLDTRTSLEIMGVFQRLNEQGLTIIMVTHEHDIARCATRTVVMR
                     DGRIISDSPVTDRLNAGEALRQLPPQEVAGA"
     gene            2387..3604
                     /locus_tag="AC16BC_03120"
     CDS             2387..3604
                     /locus_tag="AC16BC_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3240639.1"
                     /translation="MRPSATAKLAWRALRRNPMRSALTALGIVVGVAAVIAMVAVGNG
                     AKAQVEAQIASLGRNVIQVFSGSTTSSGIRTGWGGAGTLTIEDMEAIAREIPGVAGAS
                     PEVRSVAQVVAENQNWSTQILGVSPEYFAIRQWALVEGEPFTERDVRAAAKIAVIGRT
                     TAQQLFAGSSPVGRIVRIKNVPFVVVGLLAPRGFSMHGTDQDDVLVLPYSSAMKRVLG
                     VTTVRSITIQAAGASLLDSLSDQITSLLRQRHRIGPGRDDDFTVRTQEDIAEAATETS
                     RVMTTLLGAIAGVSLIVGGIGIMNIMLVSVTERTREIGIRMAVGARARDILTQFMLEA
                     VALSMVGGAIGIVLGIVAAKVLSALAEWPTVISAGPIAVAVLFSAAVGIFFGFYPARK
                     ASRLDPITALRYE"
BASE COUNT          661 a         1190 c         1376 g          648 t
ORIGIN      
        1 cttgccgcca aactctcccc tgagaagtac cagctgttta ccgaggtgat gggcggggcg
       61 cgctcggagt gtaaacagat ccgtcagcag attcgcgcga gccgggagca cgcattgggc
      121 attctgaccg cgccggaatt cgatgaggcg tcgtttcagc gcgaggtcga caccttgcac
      181 gaattgcgcg gccgggtggc ggaccgactc gccggcgcca cgttggaact cgccaaacag
      241 ttcgacccgg aagaacggaa actgctcggg aagtatctgg agcggccacc gccgccctga
      301 cgggccgtcg acgtagtcgg tacgccgcgt tcgcggccgg ctggggtgtc cgcggtcggc
      361 cgcgtagtcg tcatgcacat ggatcgggat ctgatgccgt gaggcagggg agattcgcgt
      421 cggtgaaatg ggtggtggtc gtggccgtgg gcctggtggt cggcggcggt ctctggtatt
      481 ggatggcgcg caatgacgcc gccgcggagt atcagaccgc cgtggtgacg cgaggcgcgt
      541 tgacgcaagt ggtcaccgca acgggccaac tgaacccggt cgtcagcgta caggtcggca
      601 gtcagatttc cgggatcatt cagaagctgt tcgtcgactt caactcgcgg gtgaaggcgg
      661 gacaggtgat cgcccaactg gattcgtcca cttttgtcgc gaatcttcgc caggcggaag
      721 gcgaattggc caacgcgcgg gcggggctcg aattggccag ggtcaaggcg acccgggccg
      781 aggaactccg ggccaaagac ctcattccac aggctgaaca cgaccaagcc caggccgacc
      841 tgcaccaagc cgaggccgcc gtcgtcatcc gtgaggccgc cgttcgcaaa gccgcggtgg
      901 atctcgagcg ctgcaccatc gaggccccga tcgacggcgt ggtgatttcc cgcagcgtgg
      961 acgtggggca gaccgtggcc gcgagcctca gcgcgccgac gctcttcgtg atcgccaacg
     1021 acctgaccaa gatgcagatc aacgcgagcg tggccgaggc cgatatcggc ggcgtcgagg
     1081 tcggccagga cgtcaatttt acggtggacg cgtttccctc tcagcggttt cacgggcggg
     1141 tggtgcaagt gcgcaatgcg ccggtcacgg tgcagaacgt ggtcacgtac gacacgatta
     1201 tcgaagtggc caacccggaa ttgcgactca agcccggcat gaccgcgaac gtgtccatcg
     1261 tggtggcgaa ccgggagggc gtgctcacgg ttcccaacgc ggcgttgcgc ttccgtccgc
     1321 cggatgccgc gaccagcgtc gcgccgagta cggcgggcgg cgggaccaac gcaaccacga
     1381 agacgcgcgg tggtacgtca gcggggcggg ccgtatactt ggcaccccaa ggaaaggacc
     1441 ggccggcacc cgaacgggtg ccggtcaaga ccggaatcac cgacggggtc gtcaccgaag
     1501 tcctggaagg gctccaggaa ggtgatgccg tggtcatcgg agtgcgcttc gatcagtcga
     1561 actccgactc gcggtcgggc ggaagcaatc cgttcggcgg gcctcggcgg ttctaggatt
     1621 cacggtgtcg cgatgagcgg actgatcgtt ctggagcacg tgtccaaaac gtaccgcagc
     1681 ggcgacatcg acgtgcacgc ggtgcgggac gtcagcgcgg agatcgcgcg cggggaattc
     1741 gtggcggtga tgggcgcgag cggatcgggg aagtccacgt tgatgaacat cgtggggtgt
     1801 ctcgaccggc ccagcagcgg ccggtacgtg ctcgacggcg tcgacgtgtc cgggctggac
     1861 ggcgatcaac tggcggacct ccggaacgcg aagctgggat tcgtgttcca ggggtttcat
     1921 ctgctgccga ggacgtcggc cctcgaaaac gtggaactcc cgatgttgta cgcgcgcggc
     1981 gtgaacaggg cccaacagcg cgagcgcgcg atgcgggccc ttgagatcgt gggattgacg
     2041 gcccgagccg atcaccaccc caaccaactg tcggggggac aacagcagcg ggtggccatc
     2101 gcgcggtcgt tggtcaacga accggtggtg ctgctggccg acgagccgac cggcaatctc
     2161 gatacccgaa ccagcttgga gatcatgggg gtgttccagc ggctgaacga acagggcctg
     2221 acgatcatca tggtcaccca tgaacacgac atcgcgcgat gcgccacgcg caccgtggtg
     2281 atgcgcgacg gacggatcat ctcggactcg ccggtcacgg atcgtctcaa cgccggtgag
     2341 gccttgcgtc aactgccccc tcaagaggtc gcgggcgcgt gacggcatga gaccgtcggc
     2401 caccgccaaa ctcgcgtggc gcgcgttgcg ccgcaacccg atgcgctcgg cgttgaccgc
     2461 gctcggtatc gtggtgggcg tggccgccgt cattgcgatg gtggccgtcg gcaacggggc
     2521 gaaggcgcaa gtggaggcgc agatcgccag cctgggccgc aatgtcatcc aggtgttctc
     2581 cgggagcacc acgagcagcg gcattcgcac gggatggggc ggggccggca cgctcacgat
     2641 cgaggacatg gaagccatcg cgcgggagat cccgggcgtg gcgggcgcca gccccgaagt
     2701 gcgcagcgtc gcgcaggtgg tcgccgaaaa ccagaactgg tcgacccaga tcttgggggt
     2761 ttccccggag tacttcgcga tccggcaatg ggcgctggtg gagggcgaac ccttcacgga
     2821 acgcgacgtg cgcgccgccg ccaagatcgc ggtgatcggc cgaaccacgg cgcaacagct
     2881 cttcgcgggg agcagcccgg tcgggcgcat cgtgcggatc aagaacgtgc cgttcgtggt
     2941 ggtcggcctg ctcgcgcccc gaggattttc catgcacggc acggatcagg acgacgtgct
     3001 cgtcctgccg tacagctccg cgatgaagcg ggtgctgggc gtgacgacgg tgcgttcgat
     3061 cacgattcaa gccgcgggcg cgtcgctcct ggactccttg tcggaccaaa tcacgtccct
     3121 gttgcgtcaa cgtcaccgga tcggcccagg tcgggacgat gatttcacgg tccggaccca
     3181 ggaggacatc gcggaggccg cgaccgagac ctcccgcgtg atgaccacac tcctgggcgc
     3241 gatcgccggc gtctcgctga tcgtcggagg catcgggatc atgaacatca tgctggtgag
     3301 cgtgaccgag cgcacgcgcg aaatcgggat tcgcatggcg gtgggagccc gcgcgcggga
     3361 cattctgacg cagttcatgc tggaggccgt tgcgctcagc atggtgggcg gggccatcgg
     3421 gatcgtgttg ggcatcgtgg cggccaaggt gctgtccgcg ctggcggagt ggcccacggt
     3481 gatctcggcc ggaccgatcg cggtggcggt cctgttcagc gcggcggtcg gaatcttctt
     3541 cggcttttat ccggcgcgaa aggcgtcccg cctcgatccc atcaccgcgc ttcgctatga
     3601 gtgaggggag cctgtccatg aggggtcatc tgcggcgttg tcgctgcgcg tccgctcctc
     3661 acgtacgacc cagtacgctg cggtgcgggc gctcgctccg ccttgcagct ggcgcgctcc
     3721 tgaacaggcg ccgcgctgcg aatagcgcgg agcgtaccta gatgctctct gtcgtcctta
     3781 ttcccctcct gccgttagtc gccgttctga tcaacggcgt gttcgggcgt aagtatctca
     3841 aggaagcctc gcactatttt gcggtcgggt cggtc
//
LOCUS       DBNGTT010000135         2951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_794542, whole genome shotgun sequence.
ACCESSION   DBNGTT010000135 DBNGTT010000000
VERSION     DBNGTT010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_794542"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..744
                     /locus_tag="AC16BC_03125"
     CDS             34..744
                     /locus_tag="AC16BC_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3240640.1"
                     /translation="MAPVWSTTLRPDTMIGQPLEKADHEQATMRGRKSLLGTLILCRH
                     GATDYHPDRFYEAGDGPSLSSEGLGHAEALGRWFERQGLPVAGLYVSPTLRTRQTAAS
                     IERALGVQGTAWPELTERSMGRWNGRLVDEVRRDDPDGWMAWKADPLGFTPMGGESLE
                     TFGQKVEATVADLIARHPGGIIVAVTHVGTIRAALCAALGCALEHGKRLVIEPGSLTR
                     MDYTTGWPNVVWMGVQPT"
     gene            787..1110
                     /locus_tag="AC16BC_03130"
     CDS             787..1110
                     /locus_tag="AC16BC_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240641.1"
                     /translation="MCNTHSNGHAHGVRSDMDKAVADLRDDLIEELKMINKYEGQIPV
                     IASEDFPGVDDVVHVLGHIRDEHKHAVATLMEFIAKMDAGQKAAAGHGHEEEHAHGSD
                     HAHHH"
     gene            1220..2593
                     /locus_tag="AC16BC_03135"
     CDS             1220..2593
                     /locus_tag="AC16BC_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240642.1"
                     /translation="MGGRHFQLRWGVVLALVIVDGLWHDAWAIPAFTRKFDLPCSQCH
                     TIPPTLNDVGETFRTSGYRMAGLAETVPEALREQVRKELPEDLHPAYLPLSARAVMGY
                     RYRSRDHQDTDLGEAKLQTRTSGLERFDLMAGGLLTPDVNFYLTYQPTVTNADLETSA
                     GQDGRVESAWIRFNELPFGVLKWNLKVGSFELDVPASRYRRYTLSTYPIFGYFSPGSA
                     AADDPDTTLDWSRAQLGGEASGHGPGNTSLSLALINGTNGHADSNKAFDYYVRGTLPV
                     AENRFGGFLYWGTAPSNFQFTPAGDPIAGTGISNHTFYRVGLDGDVKHAPLRVLGLLA
                     YGSDSAGLFGGSDPQRASFGGGYIEVQYDLLKERSLMGVLRYDLIRNSGRFLFLDFQQ
                     GDARTDAQKGDLDAITLAARYDLIETTRLSLIIHGEYSHEKTKGTSVDGHDETDNRMT
                     VAVDLML"
     gene            2622..>2951
                     /locus_tag="AC16BC_03140"
     CDS             2622..>2951
                     /locus_tag="AC16BC_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240643.1"
                     /translation="MKLAISSILCAGMTLAAVGSVAEDASLVIVSPEDGAVVESSNVP
                     IETRFVRGTRGDHLHVYVDGVFAKTTKRETLTVWDVRDGEHTIEVRAASREKDEKGVE
                     HKEIGVNA"
BASE COUNT          574 a          807 c         1022 g          548 t
ORIGIN      
        1 cgatgtggat gttcatgcga gaacgagcct gagatggcgc cggtctggtc gactacgttg
       61 cgccccgata ccatgattgg tcagccgctg gaaaaggcgg atcacgaaca ggccaccatg
      121 cgcggccgca aatcgctctt gggcacgctg atcttgtgtc ggcacggggc cacggattat
      181 catccggatc ggttttatga ggccggcgac gggccgtccc tcagttcgga agggcttggt
      241 catgccgagg ccttggggcg atggttcgag cggcaagggc ttccggtcgc ggggctctac
      301 gtcagcccga cgttgcgaac gcgtcagacc gcggcgtcga ttgaacgggc gttgggggtt
      361 cagggaacgg cctggcccga gttgacggaa cgcagcatgg gccggtggaa tggtcggttg
      421 gtcgacgaag tcaggcggga cgatcccgac ggctggatgg cctggaaggc cgacccgttg
      481 ggatttacgc ccatgggcgg agagtcgctg gagacattcg gccaaaaagt tgaagcgacg
      541 gtggcggatc tgatcgcccg gcatcccggc ggcatcatcg tggccgtgac gcacgtcggc
      601 acgatccgcg ccgcgctctg cgcggccttg ggctgcgcat tggaacacgg caaacgtctg
      661 gtgatcgaac ccggcagtct gacccggatg gattacacca ccggctggcc gaatgtggtc
      721 tggatgggcg ttcaacccac gtagcgcggg cgtacccgcg gatttcttct cacgaggagg
      781 tgtcggatgt gtaacacgca cagtaatggt cacgcccacg gcgttcgcag cgacatggat
      841 aaggcggtcg ccgatttgcg ggacgacctg attgaagagt taaagatgat caataagtat
      901 gagggacaga ttccggtcat cgcgagcgag gacttccccg gggtcgacga cgtcgttcac
      961 gtgttgggtc atatccgtga cgagcacaaa cacgccgtgg ccacgctgat ggagttcatc
     1021 gccaagatgg atgccgggca gaaagccgcg gccggacacg gccatgagga ggagcacgcg
     1081 cacggctcgg atcacgcgca ccatcactga cgcgcggtcc gtaccgcatc gactgcagag
     1141 cgggaacccc ggggggcgtt caggtgaacg cccctcggtg cgactatcgg ggagtcacct
     1201 cagggaggga tcgacgcgaa tgggtggacg gcatttccaa ctgagatggg gcgtggtgct
     1261 cgcgctcgtg atcgttgacg gactgtggca cgacgcgtgg gccattcccg cgttcaccag
     1321 gaaattcgat cttccctgca gccaatgcca cacgattccc ccgacgctga atgacgttgg
     1381 agaaaccttc cgcacgagcg gctatcgcat ggccggtctg gcggagactg ttcccgaggc
     1441 gctccgcgag caagtccgca aggaactccc cgaagacctg caccccgcct atttgccgct
     1501 gagtgccagg gccgtgatgg gttatcggta ccgatcgcgc gatcatcagg acacggattt
     1561 gggcgaggca aaactccaga ctcggacatc gggcctcgag cggtttgacc tgatggcggg
     1621 cgggctgttg accccggacg tcaatttcta ccttacctat cagccgacgg tcacgaacgc
     1681 ggatctggag acgtcggccg ggcaagacgg ccgcgtggag tccgcgtgga ttcgattcaa
     1741 cgagttgccg ttcggcgtgc tcaagtggaa cttgaaggtc ggatcgttcg agctggacgt
     1801 gccggcatca aggtatcggc ggtacaccct gtcgacgtat ccaattttcg ggtatttctc
     1861 gccaggcagt gcggcggccg atgatcccga caccacgttg gattggagcc gcgcgcagtt
     1921 ggggggcgag gcgtcagggc acgggccggg gaacacctcc ctgtctctgg ccctgattaa
     1981 cgggacgaac ggccatgccg acagcaataa agcgtttgac tattacgtgc gggggactct
     2041 ccccgtggcc gagaatcggt tcggcggatt cctgtattgg ggaacggctc cgagcaattt
     2101 tcagttcacc ccggcgggcg atccgattgc cgggactggc atatcgaatc acaccttcta
     2161 tcgtgttggg ctggacgggg acgtgaagca cgcgccgctc agggtgctcg ggctgctcgc
     2221 gtacgggagc gacagcgcgg ggttgttcgg cgggagtgat ccccaacgag cgtcgtttgg
     2281 aggcgggtat attgaggtgc aatacgattt gttgaaggaa cggtcgctga tgggggtgtt
     2341 gcggtatgac ctcatccgca attcagggcg gttcttgttc ctagactttc agcagggcga
     2401 cgcgaggacc gacgcccaga agggcgatct cgacgcgatc acgctggcgg cgcggtacga
     2461 cctgatcgag accacccgct tgtcgttgat catccacggc gagtacagtc acgagaaaac
     2521 caaagggacc agcgtagacg gccacgacga gactgataac cgcatgacgg tggcggtcga
     2581 tctcatgttg taggacgaac cgcatcggga gaacgcaggt gatgaagttg gcgatttcga
     2641 gtatactgtg cgccggtatg acgctcgcgg cggtcgggag tgtggccgag gatgcctcgt
     2701 tggtgatcgt cagtcccgag gatggcgcgg tagttgagtc ctcgaatgtt ccgattgaaa
     2761 cgcgattcgt ccgcggtacg cgtggggatc atctccatgt gtacgtcgac ggggtgtttg
     2821 ccaagaccac gaaacgggaa accttgacgg tctgggacgt gcgggacgga gagcatacga
     2881 tcgaagtgcg cgcggcgagt cgggaaaagg acgaaaaggg agtcgagcat aaggagatcg
     2941 gcgtcaatgc c
//
LOCUS       DBNGTT010000136         6681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_666883, whole genome shotgun sequence.
ACCESSION   DBNGTT010000136 DBNGTT010000000
VERSION     DBNGTT010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_666883"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..2030
                     /locus_tag="AC16BC_03145"
     CDS             303..2030
                     /locus_tag="AC16BC_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HIR3240644.1"
                     /translation="MEQRSEWLSLCLRACVVACLVFLADWASAQQPPDAGSTLKDLKN
                     LNEPARPAKPAPQLEVAPPSSSAERNDEAVKLTVRAFRIVGNTVYSEQQLKTILHLTE
                     GRDLTLADLQQATGTITAYYRRHGYFLARAYLPVQEIQGGVVVIAVLEGRVGRALPEF
                     GTGLRLSESSARSFLAPIQPGSVIHERTLERPLLVLNDVPGVTVKATLAPGEKLGESD
                     VFVHVDKDGRLVSGAVQIDNWGNRFLGQYRVTPTLLLTNLAGIGDQLSVQGSISERSG
                     LLLGRLSYVLPAGHHGTTVGAGYTHLTYTLGEDFDILDAHGTAQVASLFASHPVVRSR
                     RLNLYVQSAVEYKQLEDRIDAVASDVKKRAIVWSARLSGDARDGWAGGGVNTFMLDYT
                     LGGLSMRTPDAVTQDAPPSGPDTEGLYQKVNYRLTRLQHVVKDVFFYVSASGQYAVKN
                     LDSSEKFILGGPEGVRAYPAGELPADVANLATAELRWSPSLPWIPGHAVGFLFTDGGW
                     AQINKEQFAGVSGQVNHRQLVGYGGGLTWGLADNVSVGATIAWHGHEDPTSDIDRSPR
                     VWVVGQTRF"
     gene            2046..4865
                     /locus_tag="AC16BC_03150"
     CDS             2046..4865
                     /locus_tag="AC16BC_03150"
                     /inference="COORDINATES: protein motif:HMM:NF017658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion domain-containing protein"
                     /protein_id="HIR3240645.1"
                     /translation="MLTRFGTRVMPRPGLHSLLGFAVLACVASSAFALPSDGDVVSGS
                     VSIRQVDPLSMQIYQTSDKAIVNWRGFSIAQGETLQFLQPGADAIFLNRVLGSDPSSI
                     FGQLSANGHVFLVNPNGVLFGPSASVNVGGFVASTLTIRDEDFLASRYTFTAEGYAGN
                     VVNQGQLTAAEGGYVSLIGTHVINDGVITARLGTVALGAGQRLTLDFAGDDLIRVAVD
                     EAAVDALVANGNLIRAGGGRVFLNASATNGLLNTVLNVDGVIEATSLSEHNGVIRLDG
                     GSSGVVAIHGKLDASGREANQTGGTVAVLGDKVGLFDGAAIDVSGGVGGGTVLVGGNL
                     HGQGPEANANYTYVDQGAAIHADALQQGDGGGVVVWADQVTRFGGAISARGGIESGNG
                     GLVEVSGKTGLVYQGLADLRAPSGGPGTLLLDPKNITIAVGGLDVINLADAFSELLST
                     DVTFSPASLQAALNLGNVVLQANNDITLSDPVTTLLLLTNNSLTLEAGRSIAINANLS
                     LRGDFTATANTNNAAVVESDRTANTAATFAMADNVTINTSTNGGDITITMSTGDPAGT
                     GFASGDLTIANLNAGTGDVLVSQGGQTAGSNILRTSNSLITANSVALDITNTGNTTGT
                     IGVGGAPINVAVTNLEARTQGGDIFINAPTQGLNIGSASLGGLTGLDTNGGSLTLTTL
                     GTITESETLTVAGTTTLAAGNSNDITLDSAGNDFGGTLSIGSGNTVTLVDANALNLGT
                     SAVAGNLVVTTAGAITDSGTLTVAGTATLAAGSANDITLDSALNDFLGAVSISSGRNV
                     TLVDTNALDLGASTIAGNLLALAGGPITLSGALSVGGTTDLISLLSDDVDSISNQLDS
                     AVATVQGTLPDALSGPLGGLPPVPPLDTPPIELPPILEPPGGTTDASDVLNIKNRDCE
                     ELILTERQSYVCREKSAKRPASPDE"
     gene            5417..5719
                     /locus_tag="AC16BC_03155"
     CDS             5417..5719
                     /locus_tag="AC16BC_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240646.1"
                     /translation="MSCAGLKDFSASLPALATDQGDGEKLVSIQRFDGRQVVNYVYLV
                     SGPSGAGFYLATLDAGTGRYISTGEKFVPVNPKKSGADMKNALSVQFNQAGLVPRP"
     gene            5875..>6681
                     /locus_tag="AC16BC_03160"
     CDS             5875..>6681
                     /locus_tag="AC16BC_03160"
                     /inference="COORDINATES: protein motif:HMM:NF028237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HIR3240647.1"
                     /translation="MITALLQLLNPDNYAFNIHALPTFVTSLAIFLLGLFVLARERGS
                     RISVLFLATTLAVMVWQLAFSWMYCATDGQVAFIWAKIAYVSVPLIPAVICHFTVVVL
                     GAYQRAKTLLWTGWIGSLSFSALDMTTSGLVDAVYRQWWGFYPKYSWLGLPFLAFFFY
                     MMVASLYLYWTEYWRAKPGLRKTRIRMLFISFAIAYLGSVDFQVQQGHIIYPFGFIPI
                     FGFVVVTAWTIWRYRLVDLTPSFAAAQILNTMDGAVLVSDADGNIRVINRA"
BASE COUNT         1299 a         2055 c         2017 g         1310 t
ORIGIN      
        1 gcccgccaca cgcgtgaaca cccccgagaa ggggaccggc tgcctacaca acctccgccg
       61 cgatgacgtt cgcaccggcc tagcctcatt tttttgcacc gggaacgtaa gcgcacgtta
      121 cttgacagca gtgcattgct aggctatggt tacgccgaat tccaaaaaag gataccatct
      181 aatcgttact gcaacttagg gcacccagga taccaggaca caatctcata ttagcaacct
      241 gagagcacct cacgttgacc accagatacc ggatacgatc gcaccggatt gaagagagtt
      301 tggtggaaca acgatcggag tggctgagtc tgtgtttgag agcttgcgtg gttgcctgcc
      361 ttgtgttcct cgccgattgg gcgtcggctc agcagccccc tgatgccggg agcacgttaa
      421 aggacttaaa aaatctgaac gagccggccc gtcccgccaa gccggcgcca caactggagg
      481 tcgcacctcc gtcgtcgtcc gccgaaagaa acgacgaagc cgtcaaactg acagtgcgcg
      541 ccttccgcat tgttggcaac accgtctact cagagcagca gcttaaaacc atactccacc
      601 tgaccgaagg acgcgactta acgttggcgg acctccaaca ggcaacgggg actatcaccg
      661 cgtactatcg gcgccacggc tatttcctgg cccgtgccta tttgccggtg caggaaatac
      721 agggaggtgt tgttgtcatc gcggtactgg agggtcgggt cggccgggcg ctcccggagt
      781 ttggcacagg cctgcgtttg tccgaatctt cggcgcggag tttcctggcc cctatacagc
      841 cggggagtgt catccacgag agaacgctcg aacggccctt gttggttctc aacgacgtgc
      901 cgggcgtcac cgttaaggcc acgctcgcgc cgggggaaaa gcttggcgag tccgacgtct
      961 tcgtgcacgt cgataaagat ggaaggttgg tctcgggcgc cgtgcaaatc gataactggg
     1021 gaaaccgctt tctggggcaa taccgggtga cgccaacact cttgctgacg aatctggctg
     1081 ggatcgggga tcaactgtca gtacagggat cgatctctga aaggagtgga ctgcttcttg
     1141 ggcggctctc ctacgtcctc ccggcaggac accacggaac cacggtgggt gcagggtaca
     1201 cccatctgac ctatacgctg ggggaggact ttgacatcct cgatgcacac gggaccgccc
     1261 aggtcgcaag cctcttcgcg tcgcaccccg tcgtccgttc acgccggctc aatctctacg
     1321 tccagtccgc cgtcgagtac aaacagcttg aggaccgcat cgatgctgtc gcgagtgatg
     1381 taaagaaacg tgccatagtc tggtcggcga gattatctgg agacgcaaga gatgggtggg
     1441 ctggaggtgg agtgaacacg ttcatgctgg attacacgct cgggggcctc agcatgcgga
     1501 cgccggatgc cgtgacgcag gacgccccac cgtccggacc tgacaccgag ggtctctacc
     1561 agaaagtcaa ctatcgtctc acgcggctgc agcacgtcgt taaggacgtc tttttctacg
     1621 tatcggcatc aggccagtac gccgtcaaaa acctcgactc gtccgaaaag ttcattttgg
     1681 gcgggcccga gggggtgcgg gcataccccg ccggagaact gccggcggat gtcgccaatc
     1741 tcgcgacggc cgaactccga tggagtccgt ccctgccctg gatccctggt cacgccgtcg
     1801 ggtttctgtt cacagatggt ggctgggctc agatcaacaa ggagcaattc gccggtgtca
     1861 gcggccaggt aaatcatcgg cagctggtcg gttatggcgg aggactcacg tggggcctcg
     1921 ccgacaacgt atcggtgggc gccaccatcg cctggcatgg tcacgaggac ccgacatcgg
     1981 atatcgaccg gtcgccacgc gtctgggtag tcggccagac gcggttttaa atggggagga
     2041 gagccatgct gaccagattt ggtacgcgcg tcatgccccg tcctggcctt cactctctgc
     2101 tcgggttcgc cgtgctcgca tgcgtggcga gcagcgcctt tgcgctccct tccgacggcg
     2161 acgtggtcag cggcagcgta tccattagac aggtcgaccc gctgagtatg cagatctacc
     2221 agacttccga caaagcgatc gtgaattggc gaggattcag catcgcccag ggggagacac
     2281 tgcagtttct tcagcctggt gcagacgcca ttttcctgaa ccgtgtgctc ggcagcgatc
     2341 catcgagcat cttcgggcag ttgtcggcca acggccatgt gttcctcgtc aaccccaatg
     2401 gggtgttgtt cggaccctct gccagcgtga atgttggcgg gttcgtcgcg tcaacgctca
     2461 caatccggga tgaggatttt ctcgccagcc ggtatacctt cacagcggag ggatacgccg
     2521 ggaacgtggt gaatcaaggc caactcaccg ccgccgaagg cggctacgta tcgttgatcg
     2581 gcacgcatgt gattaacgac ggcgtgatca ctgcccggct cggcaccgtg gcccttggag
     2641 ccggtcaacg cctcaccctg gatttcgccg gcgacgatct gatccgcgtt gccgtggatg
     2701 aggctgcggt cgatgccctc gtcgccaacg ggaacctgat ccgtgcgggg ggaggacgcg
     2761 tattcctcaa cgccagcgcg acgaacggct tgctcaacac cgtcctcaat gtcgacgggg
     2821 tgatcgaagc gacaagtctc tcggagcaca acggcgtcat ccggctggac ggaggctcca
     2881 gcggtgtcgt tgccatccat ggaaagttgg acgcatccgg cagggaagca aatcagaccg
     2941 gtggaaccgt tgccgttctg ggcgacaagg tcggcctgtt cgacggtgct gcgattgacg
     3001 tgtcgggagg tgtcggcgga ggcaccgtac ttgtcggtgg gaatcttcac ggccagggcc
     3061 cggaggccaa cgccaactac acgtatgtcg atcagggagc cgccattcat gccgatgcgc
     3121 tccagcaggg cgacggcggc ggcgtggtcg tgtgggccga tcaggtcacg cgattcggcg
     3181 gcgcgatcag tgcccgcgga ggcatcgagt ccgggaacgg cggcttggtc gaagtctcgg
     3241 ggaagaccgg cttggtctat cagggacttg cggatttgcg ggcgcccagc ggcgggcccg
     3301 gcactttgct gctcgatccg aaaaacatca ccattgccgt tggcgggctg gacgtgatca
     3361 acctggcgga cgcgttctcg gaactgctgt cgaccgatgt gacgttttct cccgcaagcc
     3421 tgcaggccgc cttgaatctc ggcaacgtcg tgttgcaggc caacaacgac attacgttgt
     3481 cggatcccgt gaccacgttg ttgttgctga cgaacaatag ccttacgctg gaggctgggc
     3541 gcagcatcgc catcaacgcc aatctctccc tgcgtggaga cttcaccgcc actgcgaata
     3601 caaacaatgc cgcggtggtg gagagcgatc gcacggcgaa caccgctgcg acctttgcca
     3661 tggccgacaa cgtgaccatt aacacgtcga ccaacggcgg tgacatcacg atcactatgt
     3721 cgacgggaga tcccgctggc acggggttcg cctccgggga cctcacgatc gccaacctca
     3781 atgctggaac gggcgatgtc ctggtcagtc agggaggcca aaccgccggc agcaatattc
     3841 ttcggacatc gaattcgttg atcaccgcga actcggtcgc gctggacatc accaatacag
     3901 gcaatacgac cggcacaatc ggggttgggg gtgccccgat caatgttgcg gtgaccaatc
     3961 tggaagcgcg cacgcaaggc ggcgatatct tcatcaacgc acccactcag ggcctgaaca
     4021 taggctccgc ttccctggga ggactcactg gtcttgatac caacggcggc agtctcacgc
     4081 tgacgacgtt ggggacgatt actgagagtg aaacgctgac ggtggcgggc accaccacat
     4141 tggcggcggg caacagcaac gacatcacgc tggatagcgc gggcaatgac tttggtggca
     4201 cgctgtcgat tggctccggg aacaccgtca ccttggtgga tgccaatgcc ttaaacctgg
     4261 ggaccagcgc ggtggcgggc aacctggtgg tcacgaccgc cggcgcgatc acggactcgg
     4321 gcacgttgac ggtcgcgggg accgccacgc tggcggcggg cagcgccaac gacatcacgc
     4381 tcgattcggc ccttaacgat tttcttggag cggtatcgat cagctcgggc cgcaatgtca
     4441 ccctggtcga tacgaacgcc ctggatctgg gggcgtctac cattgcggga aacctcttgg
     4501 cgctcgccgg ggggccgatc accttaagcg gagcgctcag cgtgggcggc acgacggatc
     4561 ttatatcctt gctctcagat gacgtcgact cgatctcaaa tcagctcgac tcggccgtgg
     4621 cgaccgttca gggcacgttg ccggacgcgc tgtcaggccc gctcggcgga ctaccaccgg
     4681 ttccgccgct ggatacgccc ccgatcgaac tgccgccgat tctcgagccg cccggtggca
     4741 ctacggacgc gagcgacgtt ctgaatatca agaataggga ttgcgaagag ttgatcctca
     4801 cggaacgaca gtcctatgtc tgtcgggaga aatccgcgaa gcgcccggca tcgccggacg
     4861 aataacgcag tgcgccgatg atcccgcggc caggcgtggg gaggacacca ccgtggaaaa
     4921 actatttccg atcgtcatcg tggctctcat catcatttcg atcgggatat ggagttacag
     4981 caaccagggt gagcgttctg ctgcgccgct aaacgcccca gagggcctac cagccccacc
     5041 ccccgctcgc cctgtgcccg agccgccgcc ccctccgccc ggagaagtcc acgagccggc
     5101 ccccgagccg gccgtcacgg agacccgccg atcgccatcc aagccggctg ccacgaaagc
     5161 ccgtcagccg gcgcccaaac caccagccgt ggtcgccaca aagcctgccg cgccaccatc
     5221 cgaatcccca gcaaggcagg aaagtatccc acagccggag ctgcaggcct cgccgacccc
     5281 ggttccgccg aagcctgtaa cgcaaccggc aagagctcgg ttgggtatcg tgggaacagg
     5341 ctattacacg ctggtatacg acagggtggc cgacacgtgg tatgaatgca gtactcccac
     5401 atgggatatc aaaggcatga gttgcgcagg cctaaaggac ttttccgcgt ctcttcccgc
     5461 gttggccacg gatcagggcg acggcgaaaa gctggtcagc atccaacgct ttgacggtcg
     5521 tcaggtcgtg aattacgtct acctcgtctc cggcccatcc ggcgcgggct tttatctcgc
     5581 cacgctcgac gccgggacgg gacggtacat ttccaccgga gagaaatttg tcccggtcaa
     5641 ccccaagaag agcggcgcgg acatgaagaa cgcactctcc gtccaattta accaagccgg
     5701 cttggttccc aggccgtgat cgcattccga cccacgctag gtcctggcct tcgtcatgac
     5761 gccagcctcc cgccttgtgg acacccgccg cactggatgt gatctccagg agtccgctcc
     5821 ctctccgcat ttcggacctc gcatgcgtga gacggcgcca accgtgggcc tcccgtgatt
     5881 accgcccttc tgcaattgct caaccccgac aactacgcat tcaacatcca tgcgctccca
     5941 acgttcgtca cgtcgttggc catcttcctc ctcggcctct tcgtattggc tcgggagcgt
     6001 ggctcgcgga tcagcgttct ctttcttgcg accacgctgg cggtcatggt gtggcaattg
     6061 gcattctcgt ggatgtactg cgccacggac gggcaggtcg cgtttatttg ggcgaagatc
     6121 gcgtatgtga gtgtgccgtt aattccggcg gtcatctgcc actttaccgt cgtcgtgctc
     6181 ggggcgtatc agcgtgcaaa gacgctcctg tggacgggct ggatcggttc gctgagtttc
     6241 tcggcgctgg acatgacgac cagcggattg gtcgatgccg tatatcggca atggtgggga
     6301 ttttatccga agtactcgtg gctgggcctg ccgttcctcg cgttcttctt ttacatgatg
     6361 gtagccagcc tgtacctgta ttggaccgaa tactggcgcg caaagccggg cctgagaaag
     6421 acccgcatca ggatgctgtt catctcattt gccatcgcct atctggggtc agtcgatttt
     6481 caagttcaac agggacacat catctaccca tttgggttca ttcccatttt cggctttgtc
     6541 gtggtgaccg cctggaccat ctggcgctat cgactggtag atctcacccc atcctttgcc
     6601 gccgcccaga ttctcaacac catggacggt gcggtcctgg tctcggacgc cgacgggaac
     6661 attcgtgtga tcaatcgcgc c
//
LOCUS       DBNGTT010000137         3467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1433327, whole genome shotgun sequence.
ACCESSION   DBNGTT010000137 DBNGTT010000000
VERSION     DBNGTT010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1433327"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1776)
                     /gene="rpiB"
                     /locus_tag="AC16BC_03165"
     CDS             complement(70..1776)
                     /gene="rpiB"
                     /locus_tag="AC16BC_03165"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR3240648.1"
                     /translation="MKIAIGSDHAGVGLKDRIGALLTELGQSVEDLGTNGIEPVDYPV
                     YGAKVAEAVSTGAVERGILVCGSGVGMSIVANKFPGVRAALCTDPQLARSSRAHNDAN
                     VLVLGERVLDPENGMAIVKAWLETPFDGGRHARRLQKIEELERRFHPRPERAPMSALR
                     TVDPDVYEAIQSEARREEEKIILIASENYVSPAVMEAQGSVLTNKYAEGYPAKRYYGG
                     CEFVDRAESLAIERAKAVFGAEHANVQPHSGSQANMAVYLAMLKPGDTVMGMSLAHGG
                     HLTHGSPVNFSGLLFRIVSYGVNKDTGLIDDDEVERLAVEHRPKMIIVGGSAYARIPD
                     FKRFRAAADKVGALLMADIAHPAGLIAAGLHPSPVPYADFVTTTTHKTLRGPRGGMVL
                     CKAAHARAIDKVLFPGIQGGPLMHVIAAKAVALKEAMSEDFIAYQRRTLENARALASG
                     LTSRGYQLVTGGTDTHLFLVDLRSKGLTGKDAEAALDAAGITLNKNAVPFDDKPPAVT
                     SGIRIGSPSVTSRGMGVSEMEQIAAMIDRVLTKGSDPAVIADTAASVKRLCERFPVYK
                     RH"
     gene            complement(1845..>3467)
                     /gene="acs"
                     /locus_tag="AC16BC_03170"
     CDS             complement(1845..>3467)
                     /gene="acs"
                     /locus_tag="AC16BC_03170"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR3240649.1"
                     /translation="YWDLFCEVRKFANVLKQLGVGKGDRVGIYLPMIPEAAIAMLACA
                     RIGAIHSVVFGGFSPESLRDRMNDAEAKVVITADGGYRRGQVVALKRNTDRALEGVPS
                     VRHVVVVQRHPGGAVDETFADMQEGRDHWWHHLMQHASMDSEPEPMDAEDVLFILYTS
                     GTTGKPKGIVHTTGGYLTGTYATTKLVFDLKEDDVYWCTADIGWVTGHSYVVYGPLAN
                     GATVVMYEGAPTHPQPDRLWEIVDKYRVTVFYTAPTAIRALLNLGDEWPKRRELKSLR
                     LLGTVGEPINPEAWMWYYEVIGKSRCPIVDTWWQTETGAIMITPLPGATPTKPGSATR
                     PFPGISAEVVSKDGKPTKPNEGGYLVITKPWPSMLRTIYGDAERYKKQYWSEIPGAYF
                     TGDGARRDKDGYFWVMGRVDDVINVAGHRLGTMEIESALVSHEAVAEAAVVGRPDEIK
                     GTGICAFVTLEHTHQPSEELRSALRAHVVKEIGALARPDDIRFTENLPKTRSGKIMRR
                     LLRDIAAGKETIGDVTTLEDLAVLAKLRQDEE"
BASE COUNT          535 a         1142 c         1124 g          666 t
ORIGIN      
        1 accggagaaa gttccgttca agcgattcgc tctggcgccg tttcaccggt tcaccggctc
       61 gccctaccgt cagtgcctct tgtagacggg aaaccgctcg cacaaccttt tgacgctcgc
      121 ggcggtgtcg gcgatgacgg ccggatcaga gcccttggtc aacacgcgat cgatcatggc
      181 cgcgatctgc tccatctccg agacgcccat gccgcggctg gttacgctgg gggatccgat
      241 gcggatgccg ctggtgacgg ccggcggttt gtcgtcgaac ggcaccgcgt tcttgttcaa
      301 cgtgatcccc gcggcatcca acgcggcttc cgcgtccttg ccggtcagcc ccttggagcg
      361 caggtccacg agaaacaggt gcgtatcggt gccgccggtg acgagttgat acccgcgcga
      421 ggtcagcccg gacgccagcg cgcgcgcgtt ctccagggtg cggcgttggt aggcgataaa
      481 gtcctcggac atggcctcct tgagcgccac ggccttcgcg gcgatcacgt gcatcagcgg
      541 cccgccctgg atgccgggga acaggacctt gtcgatggct ctggcgtgcg cggccttgca
      601 gaggaccatg cccccgcgcg ggccgcgcag ggtcttgtgc gtggtggtgg tcacgaaatc
      661 cgcgtagggc accgggctgg ggtgcaggcc cgcggcgatc aagccggccg ggtgcgcgat
      721 gtccgccatc agcagcgcgc ccaccttgtc cgcggcggcg cgaaagcgct tgaagtcggg
      781 gatccgggcg taggcgctgc cgcccacgat gatcatcttg gggcggtgct ccacggccag
      841 gcgttcgacc tcgtcgtcgt cgatcagtcc cgtgtccttg ttcaccccgt acgagacgat
      901 gcggaacaag agacccgaga agttcaccgg gctgccgtgg gtcagatgcc cgccgtgcgc
      961 gaggctcatg cccatgaccg tgtcgccggg tttgagcatg gccaggtaca ccgccatgtt
     1021 ggcctgggac ccggagtggg gttggacgtt ggcgtgctcc gcgccgaaca cggccttggc
     1081 ccgttcgatc gcgagcgatt ccgcgcggtc cacgaactcg cacccgccgt agtaccgttt
     1141 ggccggatac ccttcggcgt atttgttggt cagcaccgac ccctgcgctt ccatcaccgc
     1201 ggggctgacg taattttccg acgcgatcag gatgatcttc tcttcctcgc gacgggcttc
     1261 tgactggatc gcttcgtaca catcgggatc aacggtgcgt aacgcggaca tgggcgctct
     1321 ttcgggacga gggtggaagc gacgttcgag ttcttcgatc ttctgaagcc gtctggcgtg
     1381 gcgtccgccg tcgaacggcg tctccagcca ggctttgacg atggccatgc cgttctcggg
     1441 atcgagcacg cgctcgccga ggacgaggac attcgcatcg ttgtgcgcgc ggctggagcg
     1501 ggcaagctgc ggatccgtgc acaacgcggc gcgcacgccg gggaacttgt tggccacgat
     1561 ggacatgccc acgccggacc cgcacaccag gatgccgcgt tcgaccgcgc ccgtggacac
     1621 ggcctcggcc accttggccc catagaccgg gtaatccacg ggctcgatgc cgttggtccc
     1681 cagatcctcg acggattgac ccagttcggt caacaaggcg ccgatgcggt ctttgagccc
     1741 gacgcccgcg tggtcactgc cgatcgctat cttcatggtt aaatcatcat aatcaatcgg
     1801 agcgcgggta atcaatccgg agcagggcac ggcatgccgt gcccctactc ttcgtcttga
     1861 cggagcttcg ccagtaccgc gaggtcttcc aacgtcgtca cgtcgccgat ggtctccttg
     1921 cccgcggcga tgtcgcgcag caaccgccgc atgatcttgc cgctgcgggt cttgggaagg
     1981 ttctccgtga accggatgtc gtccggacgg gccaatgccc cgatctcctt gaccacgtgg
     2041 gcgcgcaagg cggagcgcaa ttcctcggac ggctggtgcg tgtgctccaa cgtgacgaac
     2101 gcgcagatgc ccgtgccctt gatctcgtcc gggcggccca ccaccgcggc ctcggccacg
     2161 gcttcgtggc tgaccagggc gctttcgatc tccatcgttc ccaatcggtg gcccgccacg
     2221 ttgatcacgt cgtccacgcg gcccatgacc cagaagtacc cgtccttgtc ccgtcgcgcg
     2281 ccgtcgcccg taaaatacgc gccggggatc tcgctccaat attgtttctt gtaccgctcg
     2341 gcgtccccgt agatcgtgcg cagcatcgag ggccacggct tggtgatgac cagatacccg
     2401 ccttcgttgg gcttggtggg tttgccgtcc ttggagacca cctcggccga gatgccgggg
     2461 aacggtctgg tcgcggagcc cggcttggtg ggggtggcgc ccggcaacgg ggtgatcatg
     2521 atggcgccgg tctcggtttg ccaccaggtg tccacgatcg ggcagcggct cttgccgatg
     2581 acctcgtaat accacatcca cgcttcgggg ttgatgggtt cgcccacggt gccgagcagg
     2641 cgcaggctct tgagttcgcg gcgcttgggc cactcgtcgc ccaaattgag caacgcgcgg
     2701 atcgcggtgg gtgcggtgta gaacacggtg acgcggtatt tgtccacgat ctcccacagc
     2761 cggtcgggtt gaggatgcgt gggcgcgccc tcgtacatca ccacggtcgc gccgttggcc
     2821 aacggtccgt agaccacgta gctgtggccc gtgacccatc cgatatccgc ggtgcaccag
     2881 tagacgtcgt cctctttgag atcgaacacc agcttggtgg tggcgtacgt gccggtcagg
     2941 taaccgcccg tcgtgtgcac gatgcccttc ggctttccgg tcgtgcctga cgtgtacagg
     3001 atgaacagga catcctccgc atccatcggc tcgggctccg agtccatgga ggcgtgttgc
     3061 atcaggtgat gccaccagtg gtcgcgtccc tcctgcatgt cggcgaaggt ctcgtcgacg
     3121 gctccgcccg ggtgccgctg gaccacgacg acatgcctga ccgacggaac gccctcgagt
     3181 gccctgtccg tgttgcgctt gagcgcgacc acctggcccc ggcgatagcc gccatcggcg
     3241 gttatcacga ccttcgcctc ggcgtcgttc atgcggtcgc gcaacgactc cggcgagaac
     3301 cccccgaaga cgacggagtg aatggcgccg attcgtgcac aggccagcat cgcgatggcg
     3361 gcttcgggaa tcatcggcag gtagatgccc actcgatcgc cttttcccac cccgagctgc
     3421 ttgagcacgt tggcgaactt ccggacttca cagaagagat cccagta
//
LOCUS       DBNGTT010000138         1900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2966861, whole genome shotgun sequence.
ACCESSION   DBNGTT010000138 DBNGTT010000000
VERSION     DBNGTT010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2966861"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="AC16BC_03175"
     CDS             complement(<1..320)
                     /locus_tag="AC16BC_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR3240650.1"
                     /translation="MSDHAPNAESSFRLVSEFHPKGDQVGAIETLAERFESGVSRQVL
                     LGVTGSGKTFTMANVIARLNRPTLVLVHNKTLAAQLYHEFKTFFPGNAVGYFVSYYDY
                     YQPEA"
     gene            496..>1900
                     /locus_tag="AC16BC_03180"
     CDS             496..>1900
                     /locus_tag="AC16BC_03180"
                     /inference="COORDINATES: protein motif:HMM:NF021619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3240651.1"
                     /translation="MDGELHRFTQWKPLQDGLANATGLSLLTYGPSRAVDLNRTLCEP
                     SHDNPICRLLQDQYGQRAKCDADCGRHVAQAFATGRGAAFTCHANLHTFTVPVTDERG
                     VSMVILGGKSFSSYEDFFTFREAAQRYGVDANTLLNLVKDIQFKEPSYLESASALLEG
                     IARSVIRSLASRQRQEASAARLMTLLSIGAEMRHQLDQPSLFRLVLNTVGVLFNANTA
                     LILGADARGERFHVLEGFGVKREALARLSVPRRQGLLADVGTARGHVSCDSPVELAQA
                     GFPADVRSAHIFPLVNDGQCALAVLDTALSAEDVKVLMAFCEMFAVVLENTRLRAQVT
                     GRQQGLSTLTELMRKSASTLTVSDLFREVLERSTESVEAEQGSLMLLDEDRNELVVKA
                     IKGLNPKIVEALRIRPGEGISGIVFAEGKPLLVSNLETDERVLQEKRPRYRTRSFVSL
                     PLRADERTIGVLNVADKA"
BASE COUNT          336 a          600 c          622 g          342 t
ORIGIN      
        1 gcctcgggct ggtagtaatc gtagtaactc acgaagtagc ccaccgcgtt cccggggaaa
       61 aacgtcttga attcgtgata gagctgcgcg gcaagggtct tgttgtggac caagaccagg
      121 gtcggccggt tgagccgggc gatcacgttc gccatggtga acgtcttgcc cgatcccgtg
      181 acccccagga gcacctgccg ggaaaccccg gattcaaacc gctccgcgag cgtctcgatg
      241 gccccgacct ggtcgccctt ggggtgaaat tcggagacca gccggaacga ggactcggcg
      301 ttgggcgcgt gatccgacac ggcggcattg taccacggcc gatccggggt ccgcgcgagg
      361 ccatgaatcg tcgcaaatcc aggcagggat tgacttcgcg agcaaacggg gcgtatagat
      421 agaggtattg aaacggcgtt cccgccacgc gttcccgccg gttcccggcc gatctgtggg
      481 gttctgattc tggatatgga tggggagttg cacaggttta cccagtggaa gccgctccaa
      541 gacggcctgg ccaacgccac cgggctgtcg ctgctcacct acggaccgtc acgcgccgtt
      601 gatctgaacc gcacgctctg cgagccgagc cacgacaacc cgatctgccg cctgctgcag
      661 gaccaatacg gccagcgggc caagtgcgac gcggattgcg gtcgccacgt ggctcaggcc
      721 ttcgcgaccg ggcgcggcgc ggcgttcacg tgccacgcca acctgcacac gttcaccgtg
      781 cccgtgaccg atgagcgcgg ggtttcgatg gtgattctgg gcggcaaatc gttctcctcc
      841 tacgaagatt tcttcacgtt ccgcgaagcc gctcagcgct acggagtgga cgcgaacact
      901 ttgctgaacc tggtcaagga cattcaattc aaagagccct cgtatctgga atccgcgtcg
      961 gcgctgctgg aaggcattgc gcggtccgtg atccgcagcc tcgcctcccg ccagcgtcag
     1021 gaggcctcgg ccgctcggct catgacgttg ttgtccatcg gcgcggagat gcgccaccag
     1081 ctcgatcagc cgtccttgtt ccggctggtg ctcaataccg tgggcgtgtt gttcaacgcg
     1141 aacaccgcgc tgattctcgg cgcggacgcg cgcggtgagc ggttccacgt gctggagggg
     1201 ttcggggtca agcgcgaggc gctggcgcga ttgagcgtgc cgcggcgcca gggactgctc
     1261 gccgacgtgg gcacggcgcg cggacacgtg tcgtgcgact cgcccgttga actggcacag
     1321 gcgggattcc cggccgatgt ccggtcggcc cacatcttcc cgctcgtgaa cgacggccag
     1381 tgcgcgctcg cggtccttga taccgccctc tcggccgaag acgtcaaagt attgatggcg
     1441 ttttgcgaaa tgttcgcggt ggtcctggaa aacacgcgcc tgcgcgccca ggtgacaggc
     1501 cggcaacagg gcctcagcac gctcaccgag ctcatgcgga aatccgcgtc cacgctcacc
     1561 gtgtccgacc tgttccggga agtcctggag cgatccaccg agtccgttga ggcggagcaa
     1621 ggttcgctga tgttgctgga tgaggatcgg aacgaattgg tggtcaaggc catcaagggc
     1681 ctcaacccaa agatcgtgga ggccctgcgc attcgtcccg gtgaggggat ctccggcatc
     1741 gtctttgccg agggcaaacc cctgctggtg tcaaacctgg aaaccgacga acgcgtgctg
     1801 caggagaaac gcccgcgcta ccgcacgcgc tcgttcgtca gtttaccgct gcgcgcggac
     1861 gagcggacca tcggcgtgct caacgtggcg gacaaggcga
//
LOCUS       DBNGTT010000139         4603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_539269, whole genome shotgun sequence.
ACCESSION   DBNGTT010000139 DBNGTT010000000
VERSION     DBNGTT010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_539269"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="AC16BC_03185"
     CDS             <1..283
                     /locus_tag="AC16BC_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3240652.1"
                     /translation="FLDEFAAMAKGRVSQMAGASQSLTTREEEILCQLANGATNKEIA
                     NALNISEHTVKIHLKNILKKLHMSNRTQAAIYAQRHGLVAAAPQAGPTR"
     gene            345..794
                     /locus_tag="AC16BC_03190"
     CDS             345..794
                     /locus_tag="AC16BC_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3240653.1"
                     /translation="MQAESECEIPSKNLSDAEARRVMGRGTTIAVVGCSTNPEKPAHF
                     VPKYLLEQGYNIIPVNPSAKGEILGKTVYPSLRAVPVPVDIVDIFRPAADVPPIVDDA
                     IAIKAKAVWMQEGIVHNAAADRARAAGLQVVMNKCLKKVHGLPLEAE"
     gene            1001..1510
                     /locus_tag="AC16BC_03195"
     CDS             1001..1510
                     /locus_tag="AC16BC_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3240654.1"
                     /translation="MAEQKTLEVGEIEKAPAKREESLWTSLTKLERDMERMVEGFWGR
                     PFPLARDLERFLGFPSMREMPVEMYDDKDDLVVKAELPGVTKQDLEVTLADNRLTISA
                     EKKRETEKREKGRYMAEVGYGRCVRTLELPTGIFPDKAKASLKNGLLEIRIPRTEEAK
                     RREVKIAVE"
     gene            1580..2068
                     /locus_tag="AC16BC_03200"
     CDS             1580..2068
                     /locus_tag="AC16BC_03200"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR3240655.1"
                     /translation="MSVTKRLKDYLDENNVEYEVIAHPPTLSAPATAEALHVSGKTVA
                     KVVMIKDGPADVMTVLPASLKVDVLRIQEVLGHRWNHRIRLATESEFANLFPDCEVGM
                     MPPFGNLYGLDVLVDSSLTEDDEIVFPAGNSRESIRMRYDDYERLVVPRVVDFGSSVL
                     GR"
     gene            2238..3233
                     /locus_tag="AC16BC_03205"
     CDS             2238..3233
                     /locus_tag="AC16BC_03205"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3240656.1"
                     /translation="MSGKRRVVIVAIVVAAAAIAWFTARRGDHAGDGLIASGTVEATD
                     AQLGFQTAGRVSDIAAREGDVVKAGDELAHLDRTEMDARRAQAAAEAAAARAMLRESE
                     RGFRSEEVEQGRAALAAATERLNDARRDLDRVERLREGGAASQESLDKARTAFDVATS
                     QQRQAQEQVRMLERGTRAETIEARRAQLAQAEAAVATIDATLANMTVRAPFAGLVTVR
                     HREPGEIVSAGAPILTVMNPDDRWVKIYVPETRIGAVRVGQPATITTDTFPDKPYRGT
                     VVFVASEAEFTPKTVQTAEERVKLVYAVKVRITDDPTRDLKPGMPADVHLGEPPP"
     gene            3230..4168
                     /locus_tag="AC16BC_03210"
     CDS             3230..4168
                     /locus_tag="AC16BC_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240657.1"
                     /translation="MSDDATAIRVEGLTRRFEAVTAVDGLTFDVRRGELFGLVGPDGA
                     GKTTTLRMLAGVLRPTAGDAVINGVSVGRDPEAVKPDLAYMSQRFGLYTDLTVLENLT
                     FYADLYQVPREERRARLDRLFRFSNLEPFQHRLAGQLSGGMRQKLGLSCALIHQPKVL
                     LLDEPTFGVDPVSRRDLWLIVHDMVAHGVTAVVSTAYMDEAERFDRVALIQSGTLLAM
                     DTPEALQRALSDRVLMVRVDRVRDAKVRVASLPAVSRAVAFGDRLHVTVASAARDAAV
                     VEAALRESGFAVQEIRMIAPSMEDVFIDRLAGVGRT"
     gene            4212..>4603
                     /locus_tag="AC16BC_03215"
     CDS             4212..>4603
                     /locus_tag="AC16BC_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3240658.1"
                     /translation="MSVRSDSPDPAVAVSRLTKVFNRFTAVDAVSFEVRRGEIFGFLG
                     PNGAGKTTTIKMLAGLLLPTSGGGTVAGLDLLTETEAIKRRVGYMSQLFSLYGDLTVE
                     ENIAFFSGLNNVPAARRGERRDWVLEMAG"
BASE COUNT          852 a         1391 c         1658 g          702 t
ORIGIN      
        1 gtttctggac gaattcgcgg ccatggccaa ggggcgagtc tcgcaaatgg ccggagcgtc
       61 gcagtcgctc accacgcgcg aggaggaaat cctgtgtcag ttggcgaacg gagccacgaa
      121 caaggaaatc gccaacgcgc tcaatatttc cgagcacacc gtcaagattc acttgaagaa
      181 tattctcaag aaactgcaca tgagcaatcg cacgcaagcc gcgatctatg cgcagcgcca
      241 cgggctcgtg gccgccgcgc cgcaagccgg accgacgagg taggtgtcgg gagacgagaa
      301 gacagcgaat cggcgaaccg gagaatcggg gaagagggga gggtatgcag gcagaatcgg
      361 agtgtgagat cccgagcaag aacctctcgg acgccgaggc gcggcgcgtc atgggacgag
      421 ggaccaccat cgcggtggtg gggtgttcga ccaatcctga aaagcccgcg cacttcgtgc
      481 ccaagtattt actggaacag ggctacaaca ttattccggt gaacccctcg gccaaggggg
      541 agattctggg gaaaaccgtg tacccgtcgt tgcgggcggt gccggtcccg gtggacatcg
      601 tcgatatctt caggccggcc gcggacgtgc cgccgatcgt ggacgacgcg atcgcgatca
      661 aggccaaggc ggtctggatg caggaaggca tcgtgcacaa cgccgcggcc gaccgcgcca
      721 gggccgcggg cctacaggtc gtcatgaaca agtgtttgaa aaaagtgcac ggcctcccac
      781 tggaggccga atgaagatgg cggcccgcag ggccgtcggc tcacttggcg cagcaaagat
      841 ccaggccgcc tctcccggtg gcgggtaacg tcggtcacac gcatcccccc accctcccct
      901 cccccctcaa ggggggaggg gagggtgggg gtggctcttt ggatgttctg aacggggggc
      961 ttgggttctt cgcttcatca taatatggat ggaggacgtc atggcggaac agaaaacgct
     1021 cgaggtgggg gaaattgaaa aggctccggc aaaacgcgaa gagtcgttgt ggacgagcct
     1081 gaccaagctg gaacgggaca tggagcggat ggtcgagggc ttctggggac gaccgttccc
     1141 gttggcccgg gatctcgagc gcttcttggg attcccctcg atgcgcgaaa tgcccgtgga
     1201 aatgtacgac gacaaggatg atctggtggt gaaggcggag ctgcccggcg tgaccaagca
     1261 ggatctggag gtcacgctcg cggacaaccg gttgaccatc tcggcggaga aaaaacggga
     1321 aaccgagaag cgagaaaaag gccggtacat ggccgaggtg ggatacggcc gctgcgtgcg
     1381 gacgctcgaa ctgccgaccg ggatctttcc ggacaaggcc aaggcgtcgc tgaagaacgg
     1441 cctgctcgag atccggattc cgcggaccga ggaggccaag cggcgcgagg tgaagatcgc
     1501 ggtggagtag acagcgatac gtggggcaaa tcccccccag ccccctttgt caaagggggg
     1561 tgacaatgag gagccgaaga tgagcgtgac caagcggtta aaagactacc tcgatgagaa
     1621 caatgtggag tacgaggtca tcgcgcaccc gcccacgctc agcgcgccgg caacggccga
     1681 ggccctgcac gtgtcgggca aaaccgtggc gaaagtggtg atgatcaagg acggcccggc
     1741 cgacgtgatg accgtgttgc cggcgagcct gaaggtcgac gtgctccgca tccaggaggt
     1801 gctcggccac cggtggaacc atcgcatccg gctggccacg gaatccgaat tcgccaacct
     1861 gttccccgac tgcgaggtcg ggatgatgcc gccgttcggc aacctctatg gcttggacgt
     1921 gctggtcgat tcgtcgctga ccgaggacga cgagatcgtg ttcccggccg ggaattcccg
     1981 cgagtcgatt cgcatgcggt acgacgacta cgagcggctg gtggtcccgc gcgtggtgga
     2041 tttcgggtcg agcgtgctcg gccgctgatc cgaccacggc gacggggagt cacgtcgctc
     2101 cgacgatgaa cgaggcgcgc cgtcctttgg aaggacggcg cgcctttctt gtgcgcggat
     2161 tgattccccg gcgcgcgccc ggtatgctgc ccgcaaccat cgagtccgag tccacgcgcc
     2221 gcggacggag aatccgagtg agcggaaaac gaagagtcgt gatcgtggcg atcgtggtcg
     2281 cggcggccgc gatcgcatgg ttcacggccc gccgtggcga ccacgccggc gacgggctga
     2341 tcgcgtcggg gaccgtggaa gcgaccgacg cgcaattggg gtttcaaacc gcggggcgcg
     2401 tcagcgacat cgcggcgcgc gagggcgatg tggtgaaggc cggcgacgag ttggcccatc
     2461 tcgaccggac cgagatggac gcgcgccgcg cccaggccgc ggccgaggcg gcggccgccc
     2521 gcgccatgct ccgggagtcg gagcgcgggt ttcgcagcga ggaagtggag caggggcggg
     2581 ccgcgctcgc ggccgcgacc gaacggctca atgacgcgcg ccgggatctg gaccgcgtgg
     2641 aacggctgcg cgagggcgga gccgccagcc aggagagtct ggacaaggcg cgcaccgcgt
     2701 tcgacgtggc gaccagccag cagcgtcagg cgcaggagca ggtgcggatg ctggagcgcg
     2761 ggacgcgagc ggaaaccatc gaggcccggc gcgcgcaatt ggcccaggcc gaggccgcgg
     2821 tggccaccat cgacgccacg ctggccaaca tgacggtgcg ggcgcccttt gccgggctgg
     2881 tgaccgtgcg gcatcgcgaa cccggcgaga tcgtgtcggc cggcgccccg atcctgaccg
     2941 tgatgaatcc ggacgaccgg tgggtcaaga tctacgtccc ggagacccgg atcggcgcgg
     3001 tgcgcgtcgg ccagcccgcg accatcacca ccgacacgtt ccccgacaaa ccctaccgcg
     3061 gcaccgtggt gttcgtcgcg agcgaagcgg agttcacgcc caagacggtg caaaccgcgg
     3121 aggagcgggt caaactggtc tacgcggtca aggtgcggat caccgacgat ccgacccgcg
     3181 acctcaagcc cggcatgccg gccgacgtcc atctgggtga accgccgccg tgagcgacga
     3241 cgcgaccgcg atcagggtcg agggcctgac ccgtcgattc gaggccgtga ccgcggtgga
     3301 cggcctgacg ttcgacgtcc ggcgcggaga attgttcgga ttggtcgggc ccgacggcgc
     3361 gggcaagacc acgaccctcc gcatgttggc cggcgtcctc cgccccaccg cgggcgacgc
     3421 ggtgatcaac ggggtcagcg tggggcgcga ccccgaggcg gtcaaacccg accttgcgta
     3481 tatgtcgcag cgattcggcc tgtacacgga tctgacggtc ctggagaacc tgacctttta
     3541 cgcggatctc taccaggtgc cgcgggagga gcggcgggcg cggctcgacc ggctgtttcg
     3601 tttttccaac ctcgaaccgt tccaacaccg gctggccgga cagctttccg gcggcatgag
     3661 acagaaactg gggttgtcgt gcgcgctgat ccaccaaccc aaagtcctgc tgttggacga
     3721 acccacgttc ggggtggacc ctgtctcgcg gcgggacctg tggttgatcg tgcacgacat
     3781 ggtcgcgcac ggggtcacgg ccgtggtgag caccgcgtac atggacgagg cggagcgctt
     3841 cgaccgggtg gcattgatcc aatcgggaac gctgctggcc atggatacgc cggaggcgtt
     3901 gcagcgcgcg ttgagcgacc gcgtgttgat ggtgcgggtg gatcgcgtcc gcgacgcgaa
     3961 ggtccgggtg gcgtcgttgc ccgcggtcag ccgcgcggtg gcgttcgggg accgcttgca
     4021 cgtcacggtc gcatcggcgg cacgcgacgc cgcggtcgtc gaggccgcat tgcgcgagtc
     4081 cggattcgcg gtccaggaga tccgaatgat cgcgccgtcg atggaagacg tgttcatcga
     4141 ccggctggcg ggcgtcgggc ggacgtgacg cgtaggggca cggcatgccg tgcccctact
     4201 aggagcggca gatgagcgtg cggtcggatt cgccggatcc cgcggtcgcg gtttcgcggc
     4261 tgaccaaggt gttcaaccga ttcaccgcgg tcgacgccgt gtcgttcgag gtgcggcgcg
     4321 gagaaatctt cggttttctc gggccgaacg gcgcgggaaa gaccaccacc atcaagatgc
     4381 tcgccggact cctgctgccg acttccggcg gcgggacggt cgcgggactc gatctgctca
     4441 ccgagaccga ggccatcaag cgacgcgtcg ggtacatgtc ccagttgttc tcgctgtacg
     4501 gcgatctgac ggtcgaggag aacatcgcgt ttttctccgg gctcaacaac gtgcccgcgg
     4561 ccagacgcgg ggagcggcgc gactgggtgt tggagatggc ggg
//
LOCUS       DBNGTT010000140         1668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2711508, whole genome shotgun sequence.
ACCESSION   DBNGTT010000140 DBNGTT010000000
VERSION     DBNGTT010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2711508"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..848)
                     /locus_tag="AC16BC_03220"
     CDS             complement(69..848)
                     /locus_tag="AC16BC_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240659.1"
                     /translation="MFEEPYRWTEAVGNRRAYVDAQFAQGSPVAALSYRDGVVLATFQ
                     RGIPKLYEVYDRIAMGGLGHPADLESLRGAALDMAHVEGFNRAPSDVTAMRLVKYGLA
                     PIAKSAYEELFRAPLLARVVLAQVGASRAQDVLVTLDYDGGFESVSGRAAVAPSSEAA
                     QAMLARLDAAGPWDDAPLAQALPAALRAWAVGDLAQSADFAAPAPDDARLTEHLKACC
                     KDRRIEVAVLDRTVPGASKYRALTPGEVSQILVPWLGEAVR"
     gene            complement(841..1593)
                     /locus_tag="AC16BC_03225"
     CDS             complement(841..1593)
                     /locus_tag="AC16BC_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HIR3240660.1"
                     /translation="MKAQGYGWTPQVTSGAIDPAAITHGTTVLAMKYRDGVIVAGDRR
                     ATAGNVVMHDRTEKVLDLDRHSVLAIAGVPATAFEMARVLDHSFKYYRRSQLQDLSLE
                     GKLRALSKLLRENVPMAVQGIGAVAPIFAGYDLDRGEAKIFFYDILGAEFEGVEYAVS
                     GSGSPTIRGIVYYLHRWGPQRVADLGADDATVLALRLLDSAAEFDSATGGVKGTDGMY
                     PVVKLITRDGIAPVPAEHLRRLYADQVEARDV"
BASE COUNT          282 a          615 c          512 g          259 t
ORIGIN      
        1 gtccgtcttc gccaggaccc cgtattcggt ttcaacgccg acgatgcggt cgagcatgga
       61 gcgtcagcct aacgaaccgc ttcgcccagc cacggcacca ggatctgcga cacttcgccg
      121 ggcgtgagcg cgcggtactt ggaggctcct ggcacggtgc ggtccagcac cgcgacctcg
      181 atccggcggt ccttgcaaca cgccttgagg tgttcggtca gccgcgcgtc gtccggcgcg
      241 ggcgcggcga agtccgcgga ctgcgccaga tctcccacgg cccaggcgcg caacgcggcg
      301 ggcagggcct gcgcgagcgg cgcgtcgtcc cacggtccgg ccgcgtccaa ccgcgcgagc
      361 atggcctgag ccgcctcgga cgacggcgcc accgccgcgc gtccggacac ggactcgaac
      421 ccgccgtcat aatccaatgt caccaagaca tcctgggcgc gcgaggcccc gacctgggcc
      481 aacacgaccc gcgccagcag cggagcgcga aacaattcct cgtacgcgct tttggcaatg
      541 ggcgcgaggc cgtatttgac caggcgcatg gcggtcacgt cggacggcgc tcggttgaac
      601 ccttccacgt gcgccatgtc cagtgcggcc ccgcgcagtg attccaggtc agccgggtgg
      661 ccgaggccgc ccattgcgat gcggtcatac acctcgtaga gcttcggtat cccgcgctga
      721 aacgtggcaa ggaccacgcc gtcgcggtac gacaaggccg ccaccgggct gccctgcgcg
      781 aactgcgcgt caacgtacgc gcgccggttg ccgaccgcct cggtccagcg atacggctcc
      841 tcaaacatcg cgcgcctcca cctgatcggc gtacaaccga cggaggtgtt cggccggaac
      901 cggcgcgatc ccgtctcgcg tgatcaattt cacgacggga tacatgccgt cggtgccctt
      961 gaccccgccg gtggcggagt cgaactccgc ggcgctgtcc aacaatcgga gcgccaatac
     1021 ggtggcgtcg tccgcgccca gatcggccac gcgttgcggt ccccagcggt gcaggtaata
     1081 cacgatgccg cggatcgtgg gcgacccgga ccccgacacc gcgtactcca cgccttcgaa
     1141 ctccgcgccc agaatgtcgt agaaaaagat cttggcctcg cccctatcga gatcgtaccc
     1201 ggcgaagatc ggcgccaccg cgccgatgcc ctgtaccgcc atgggcacgt tctcacgcag
     1261 gagcttggac aacgcgcgca atttaccctc caggctcaag tcctggagct ggctgcggcg
     1321 atagtacttg aacgagtgat ccagcacccg cgccatctcg aacgcggtgg ccggaacccc
     1381 cgcgatggcc agcacgctgt gccggtccag atccagcacc ttctcggtcc gatcgtgcat
     1441 caccacattg cccgcggtgg cccgccgatc cccggcgaca atgacgccgt cacggtactt
     1501 catggccagc accgtggtcc cgtgcgtaat cgccgccgga tcaatggccc cgctcgtgac
     1561 ctgcggcgtc cacccgtacc cctgcgcctt caaaacctct agaaaatccc ctcgcaccga
     1621 ttgcctctcc cgtaggggcg cacggcgtgc gcccgatgct tattcgaa
//
LOCUS       DBNGTT010000141         5766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2838900, whole genome shotgun sequence.
ACCESSION   DBNGTT010000141 DBNGTT010000000
VERSION     DBNGTT010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2838900"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /gene="atpD"
                     /locus_tag="AC16BC_03230"
     CDS             complement(<1..1283)
                     /gene="atpD"
                     /locus_tag="AC16BC_03230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR3240661.1"
                     /translation="MQTGQIVQVIGPVVDVEFPRGALPLIYNALKVEQPADEKAGRAA
                     INLTLETAQHLGENRVRAVAMSSTDGLIRGMEVSDTGAPISMPVGPEVLGRILNVVGD
                     PVDQKGPVKAKTRLPIHRPAPSFEEQNTSAEVFETGIKVVDLLEPYTRGGKTGLFGGA
                     GVGKTVIIQELIHNIAKEHGGYSVFAGVGERTREGNDLWLEMKESGVIDRTALVYGQM
                     NEPPGARLRIGLSGLTIAEYFRDQEGRDVLLFVDNIFRFTQAGSEVSALLGRMPSAVG
                     YQPTLSTEMGTLQERITTTKKGSITSVQAIYVPADDLTDPAPANAFAHLDATTVLSRQ
                     LAELGIYPAVDPLDSTSRILDPKIVGEEHYNVARSVQSILQRYKDLQDIIAILGMDEL
                     SEDDKLTVARARKIQRFLSQPFFVAEAFTGTPGRYV"
     gene            complement(1318..2181)
                     /gene="atpG"
                     /locus_tag="AC16BC_03235"
     CDS             complement(1318..2181)
                     /gene="atpG"
                     /locus_tag="AC16BC_03235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR3240662.1"
                     /translation="MPSLQQTRRRIGSVKNTQKITKAMKLVSAAKLRRAQERILKARP
                     YAQKMEALLANLGGRVSRDVHPLLAVRPIKKVDIVVVAADRGLCGPFNAQIMRRTMEL
                     ARVQQAQGRQVSLTCVGRKSRDFFRRRQIPIRHTAVGVFDKLSFEHAAELAKNVMDGY
                     LGGAMDECWLIYSEFKSAMASQIQVKRLAPIQPEGESASDVGPDYMYEPSDEALLVDL
                     VPRAVEIGIWRALLESAASEQGARMMAMDSASRNAKEMIAKLTLVYNKTRQASITKEL
                     MDIVGGAEALK"
     gene            complement(2342..3865)
                     /gene="atpA"
                     /locus_tag="AC16BC_03240"
     CDS             complement(2342..3865)
                     /gene="atpA"
                     /locus_tag="AC16BC_03240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3240663.1"
                     /translation="MQMKAEEITEILKGQIKGFDGAVQAAEVGTVLQVGDGIAKIYGL
                     EHAMAGELLEFPGGVRGIVLNLEEDNIGAVLLGGDTVIKEGDTVKRTGRIAEVPVGDA
                     MIGRVIDPIGVPVDGKGPIAAKETRRVEVIAPGVVQRQPVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKGQDVICIYVAIGQKRSTVARVVSTLEAAGAM
                     AYTIVVSATASDPAPSQFLAPYSGCAIGEYFRDNGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYVHSRLLERAAKLSAELGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGADLFYSGIRPAINVGLSVSRVGGSAQIKAMKQVAGTLRLDLAQYREM
                     AAFAQFGSDLDKATQAQLARGTRMVELLKQGQYEPLSVELQIIAIFAGINGFVDDIPV
                     NEVRRFETELLKYIDGEQKALRQDIAAKKALDADLTARLKAAISEFKAKRFILRAGA"
     gene            complement(3895..4437)
                     /gene="atpH"
                     /locus_tag="AC16BC_03245"
     CDS             complement(3895..4437)
                     /gene="atpH"
                     /locus_tag="AC16BC_03245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR3240664.1"
                     /translation="MQGEVLGRRYAKALFELTKTDPQRGAIGTLLSALGEQLDTRSPL
                     ADACLNPRYPVESKRAVLAKFAERAGAGDMAVKFLELLLKKNRLSFLSAIASAYQTLL
                     DEEAGRRRVTVTAAKPLDEQEQAALKRRLESVTGRTVSLEVTVDPAVLGGVVIRSGSL
                     YYDASLKGQLERLRRELVSG"
     gene            5032..5598
                     /locus_tag="AC16BC_03250"
     CDS             5032..5598
                     /locus_tag="AC16BC_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3240665.1"
                     /translation="MKRVLAVLVSPIAMGVALLAAPVPVSAFSFNHGSLIVTKDGGGD
                     RNVNVRFSSGGSNKYEQGYFLNGDSQFRSVRDNDVKSFRDGDVVDLALYDRSTGKIHS
                     LSKERDDYEFHASMNFDERPDRHDGEVSWHFDKDDHHTNIHFEDGKDCAVPSHGVPEP
                     GSLVLIGMGLTGIALLQWRRLRAATDKI"
BASE COUNT         1054 a         1847 c         1740 g         1125 t
ORIGIN      
        1 acgtatcggc ccggcgtgcc ggtgaacgct tcggccacga agaacggttg cgacaggaag
       61 cgctggatct tgcgcgcgcg cgccacggtc agcttgtcgt cctccgagag ttcgtccatg
      121 cccaagatcg cgatgatgtc ctgcaggtcc ttgtaccgct gcaggatcga ctgcacgctg
      181 cgcgcgacgt tgtagtgttc ttcgcccacg attttgggat cgaggattcg cgacgtggaa
      241 tccaacgggt ccaccgccgg gtagatgccc aactccgcga gttggcgcga caacacggtg
      301 gttgcgtcca aatgcgcgaa cgcgttggcc ggcgccgggt cggtcaagtc gtcggcgggc
      361 acgtagatgg cctgcaccga ggtgatcgat ccctttttgg tggtggtgat ccgttcttgc
      421 aacgtcccca tttccgtgga cagggtcggc tgatagccca ccgcggacgg catacgtccc
      481 agcaacgcgg acacttccga gcccgcctgg gtgaaccgga agatgttgtc gacgaagagc
      541 agcacgtcgc ggccctcttg atcgcggaag tattccgcga tggtcagccc ggacaggccg
      601 atccgcagcc gcgccccggg cggctcgttc atctgaccat agaccagcgc ggtccggtcg
      661 atgacgcccg attccttcat ttccagccag aggtcattgc cttcgcgcgt gcgctcgccc
      721 acgcccgcga acaccgaata accaccgtgc tccttggcga tgttgtggat caactcctga
      781 atgatcacgg tcttgcccac gccggcaccc ccgaacaacc cggtcttgcc gccgcgggtg
      841 tagggttcga gcaggtcgac gaccttgatg ccggtctcga acacttcggc cgaggtgttc
      901 tgctcttcga acgacggcgc gggccgatgg atcggcagcc gagtcttggc cttgaccggc
      961 cccttctgat ccaccggatc acccaccacg ttgagaatcc tgcccaggac ctccggcccc
     1021 accggcatgg aaatcggcgc acccgtgtcc gagacctcca tgccgcggat cagcccgtcg
     1081 gtggaggaca tcgccaccgc ccgcacgcga ttttcaccca ggtgctgggc ggtttccagc
     1141 gtgaggttga tcgcggcccg gcccgccttc tcatcagcgg gctgttcgac cttgagcgcg
     1201 ttgtaaatca acggcaacgc gccacgggga aactccacgt ccacgaccgg accgatcacc
     1261 tgaacgattt gacctgtttg cacggcagtc atccttctgt gtttggagcg cgagcgctta
     1321 cttcagcgcc tcggcgccgc cgacgatgtc catcagttct ttggtaatgc tggcctgtcg
     1381 ggtcttgttg tagaccagcg tgagtttggc gatcatttct ttggcgttgc gcgaggccga
     1441 atccatcgcc atcatgcgcg cgccctgttc gctggccgcg gactccagca gcgcgcgcca
     1501 gatcccgatc tccacggcgc ggggcaccag atcgaccagc agggcttcgt ccgagggctc
     1561 gtacatataa tcagggccca catcagacgc gctctcgccc tcgggctgaa tcggcgccaa
     1621 ccgtttgacc tggatctgcg acgccatggc cgacttgaac tcgctgtaga tcaaccagca
     1681 ttcgtccatc gcgccgccga ggtagccgtc catcacgttc ttggccaact cagccgcgtg
     1741 ctcgaacgac agtttgtcga agacgccgac cgcggtatgc cggatcggaa tctgacggcg
     1801 gcggaagaag tcgcgcgact tgcggccgac gcacgtgagc gacacctggc gcccctgcgc
     1861 ctgctggacg cgcgcgagtt ccatcgtgcg ccgcatgatc tgcgcgttaa acggcccgca
     1921 caagccccga tcggccgcca ccaccacgat gtccactttt ttgatcggcc gcaccgcgag
     1981 caacgggtgc acgtcgcggc tcacgcgtcc gcccaaattc gccagcaacg cttccatctt
     2041 ttgcgcgtag ggacgggcct tgagaatccg ctcctgcgcg cgacgcagct tggccgcgga
     2101 caccaacttc atggccttgg tgatcttctg ggtgttcttg acgctgccga tgcggcggcg
     2161 ggtctgctgc agactcggca tggatcaggc gaccgttgat gagcggccat ctgcggcgtt
     2221 gccgctgcgc atccggtgct cacgtaccca cctcgtacgc tgcgctccag tgctcgcggc
     2281 gccttgcatc tggcgcgctc ctgaacgggc ttccactctc attagcaaca tagtggtttg
     2341 attaggcgcc ggcgcgcagg ataaaccgct tcgccttgaa ttcggaaatg gccgccttca
     2401 agcgcgcggt caggtcggcg tcgagcgcct tcttggcggc gatgtcctga cgcaaggctt
     2461 tttgctcgcc gtcgatgtac ttgagcagtt cggtctcgaa ccgacggact tcgttcaccg
     2521 ggatatcgtc cacgaatccg ttgatgcccg cgaagatcgc gataatctgc agttccacgg
     2581 agagcggctc gtactggccc tgcttgagca gctccaccat gcgggttccg cgggcaagct
     2641 gcgcctgcgt ggccttgtcg aggtcgctgc cgaactgcgc gaacgcggcc atttcacggt
     2701 attgcgccag gtccaggcga agcgtgcccg cgacctgctt catggccttg atctgcgccg
     2761 aaccgcccac gcgcgagacg gacaagccga cgttgatggc cggacgaatg ccggagtaga
     2821 agagatccgc gcccagatag atctggccgt cggtgatcga aatcacgttc gtgggaatgt
     2881 acgcggacac gtcgccggcc tgggtctcga tgatcggcag cgcggtcagc gatccgccgc
     2941 ccaactcggc cgacaacttg gccgcgcgct ccaaaaggcg cgagtgcacg taaaacacgt
     3001 cgccgggata cgcctcgcgg cccgggggcc ggcgcaacag aagcgacaat tggcgatagg
     3061 ccaccgcgtg tttggacaag tcgtcgtaca cgatcaacgc gtgcttgccg ttgtcgcgga
     3121 aatactcgcc gatcgcgcag ccggaatagg gcgccaggaa ctgcgagggc gccgggtcgc
     3181 tcgccgtggc cgacaccacg atcgtatacg ccatggcgcc ggcggcttcg agcgtgctca
     3241 ccacgcgcgc cacggtggaa cgcttctggc cgatcgccac gtagatgcag atgacgtcct
     3301 ggcccttttg attgatgatc gtgtcgatgg cgatcgcggt cttgccggtc tggcggtcgc
     3361 cgataatcaa ctcgcgctgg ccgcggccga tcggaatcat cgcgtcgatg gccttgatcc
     3421 cggtctgcaa cggctcgcgc acgggttggc gttgcaccac gcccggcgcg atgacttcga
     3481 cgcggcgggt ttccttggcc gcgatgggcc ccttgccgtc caccggcacg ccgatgggat
     3541 cgatcacgcg gccgatcatc gcgtcgccga ccggaacctc cgcgatgcgc ccggtgcgct
     3601 tgaccgtgtc gccttcttta atgacggtgt cgccgcccag cagcacggcc ccgatattgt
     3661 cttcttcgag attgaggacg atgccccgca cgccgccggg gaattccaac agttcgccgg
     3721 ccatggcgtg ctcaaggccg tagatcttgg cgatcccgtc tccgacttgc agcacggtgc
     3781 cgacctcggc ggcctgcacc gcgccgtcaa agcccttgat ctggcccttg agaatctcgg
     3841 tgatttcctc agccttcatt tgcatgcagg tgactccttc atgaagatcc gccgctagcc
     3901 ggaaaccaac tcgcggcgca aacgttccag ctggcccttg agactcgcgt cgtaatagag
     3961 gctgccggag cgaatcacca cgccgccgag cacggcggga tcgaccgtga cctcgaggct
     4021 gacggtccgt cccgtgaccg actccagacg gcgcttcagc gcggcttgct cctgttcgtc
     4081 caatggcttg gccgcggtca cggtcacccg gcggcgaccg gcttcttcgt ccagcagtgt
     4141 ctggtaggcg cttgcgatcg ccgagaggaa cgacaagcgg ttcttcttca acaacaactc
     4201 gaggaacttc accgccatgt ccccggcgcc cgcgcgttcg gcgaatttcg cgaggaccgc
     4261 gcgctttgat tcaaccgggt agcgtggatt gagacacgcg tccgccaacg ggcttcgcgt
     4321 atcgagttgc tcgcctaacg cggataaaag agttccgatc gcgcctcgct gtggatcggt
     4381 tttggtcaat tcgaacagcg ctttcgcgta gcgccgcccc aagacttcgc cttgcatcga
     4441 tatccccaaa acaaaggcgg tacggtagca cagcgcgttg gacgaagtca aacgttccgc
     4501 ggacctgagt cgaccggtcc gacacgatcc aaacgcacga gagacggtaa gtccaattcg
     4561 ggataaatga ctgcgtccga ccgtatgcgt tgtccgcgaa aaattggccg agagagcgac
     4621 tattcatgtc caaggcgacc tcctgtgatc gaagattcac gccgttttgg tatcagaaaa
     4681 cccgacgcgc aggaaaccgt gcttgcgaac cgccgataca tcacaaccac atgaaagtac
     4741 taataaaacc aagttgaata cacctttgaa gtcgcattgg ctcgtgttcg cgtcagaagt
     4801 ctttacaacg tgcgcgaaat tccccggtca agccacttca aaacccgacg tctatttcgg
     4861 gctgtatatt acgaaatcac tcagtaaatc atacgtctat aagactatga ctcctgacct
     4921 cagaccgtct gataaacaga atgatcttcg gtactgaact tgcgtcgtct acgtacagga
     4981 agggggctat ccagcagttg gatagccggt tgtatcgact acggggtgac gatgaagaga
     5041 gtgctggcgg ttctggtttc accgatcgcg atgggagtgg cgttgcttgc ggcgcccgtg
     5101 cccgtatcgg cgttttcctt caaccacggc tcactgatcg tcacaaaaga cggcggcggg
     5161 gaccgcaacg tcaatgtccg attctccagc ggcggctcga ataagtatga gcagggatac
     5221 ttcctcaacg gcgactcgca gttccggtcg gtgcgagaca acgacgtgaa atcgttccgc
     5281 gacggagacg tggtggattt ggctctgtac gatcggtcaa ccggcaagat ccactcgctc
     5341 tcgaaggaac gagatgacta cgagtttcac gcgtcgatga acttcgacga gcggccggac
     5401 cggcacgacg gcgaggtctc gtggcatttc gacaaggacg accaccacac caacatccat
     5461 tttgaggacg gcaaggactg cgccgttccg tcccatgggg ttccggagcc gggctcgctc
     5521 gtgctcatcg gcatgggatt gaccgggatt gcgcttctgc aatggcggcg tttgcgcgcg
     5581 gccacggaca agatctgatc tagtccagaa ccatcgcact ctaagacaac ggccggagga
     5641 aatcttcctc cggccgttgt cttttcccat gggagcgatc gaacccgcag cctcaagacg
     5701 cgactgcgtc agcgccacgc gacgatattc ggcgccttgc ggggcgtcgg agaggtgtcc
     5761 ccccgc
//
LOCUS       DBNGTT010000142         2227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2839403, whole genome shotgun sequence.
ACCESSION   DBNGTT010000142 DBNGTT010000000
VERSION     DBNGTT010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2839403"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="AC16BC_03255"
     CDS             <1..696
                     /locus_tag="AC16BC_03255"
                     /inference="COORDINATES: protein motif:HMM:NF020752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HIR3240666.1"
                     /translation="KMSKSLGNFFTIREILDKSPWERAVTAEALRYLLLGTHYRSPID
                     FSDEGLRGAKASLDGFYDLLGRLVEREGKTDGGEGNEIARRVLDRLEDDFHVAMDDDL
                     NTAQALGVLHHARAELNRALGRGLGLALAERAVQALRGIGHVLGLEFRATHRGQVPSG
                     EAHLTLTATVKGTGTVIVGRMSDEEVARLVDERNAARARRDWAAADAVRKQLADAGVV
                     LEDRPDGTTRVKR"
     gene            693..1442
                     /gene="rlmB"
                     /locus_tag="AC16BC_03260"
     CDS             693..1442
                     /gene="rlmB"
                     /locus_tag="AC16BC_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR3240667.1"
                     /translation="MSAPREEIVFGVNAVLEALRAGRLFRRILIQRGRRGGDIDDIEQ
                     AARAAGVPVHAEPREALDRATGVLKHQGVAAFLTEGAYRAFDDMLAHAGRQPGGPFLV
                     VLDGVEDPRNLGAIARTAEAAGVHGLIMPQRRAAGVTGVAMKASAGALAHLPVAQVVN
                     LARAMEQMKTAGCWLVGLDAKGDSDYTAVDYRSPLAIVVGHEGEGLRASTLKACDFRV
                     RIPMRGRVSSLNVSVAAAVVLYEALRQRTLH"
     gene            1653..>2227
                     /locus_tag="AC16BC_03265"
     CDS             1653..>2227
                     /locus_tag="AC16BC_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR3240668.1"
                     /translation="MQQQLSEITDTRETVAPVQSAVASPIVTRELAAVEALNREAEER
                     WRSKLLLEVALSRSLVSFQANKGRAVYRWFKYKEGFSAGLLEYLLNRYRITNGVLLDP
                     FAGSGTALFAAGAFGLRAEGIELLPIGQKIIETKQILDWNFLPEDAERLTCWLLEQPW
                     RNHVVEQSLNELRITRGAYPIETVSAMGKFL"
BASE COUNT          417 a          677 c          733 g          400 t
ORIGIN      
        1 aagatgtcca agtcgctggg caacttcttt acgatccgcg agatcctgga caagtcgccc
       61 tgggagcggg cggtcacggc cgaggccttg cgctacctgc tgctcggcac gcactaccgg
      121 agcccgatcg atttttccga cgaaggcctg cgcggggcca aggcgagcct tgacgggttc
      181 tacgacctgt tgggccgcct cgtggagcgc gagggcaaga ccgacggcgg ggaaggcaac
      241 gagatcgcgc gccgggtgct ggatcgcctt gaggatgatt ttcacgtcgc gatggacgac
      301 gacctcaaca ccgcgcaggc gctcggggtc ctgcaccacg cccgcgccga attgaaccgg
      361 gcgttgggcc gcggcctggg gctcgcgctc gccgagcgag cggttcaggc gctgcgaggc
      421 attgggcacg tgttgggctt ggagtttcga gctacacacc gtggccaggt tccatcaggc
      481 gaggctcatc tgaccttgac ggctaccgtg aaaggtacag gaacggtaat cgtcggaagg
      541 atgtccgacg aagaggttgc ccgattggtc gacgaacgca acgcggcgcg ggcgcggcgc
      601 gattgggccg cggccgacgc ggttcgcaag cagctggcgg acgcgggcgt cgtcctcgag
      661 gatcggcccg acggcaccac ccgtgtgaag cggtgagcgc gccgcgcgaa gaaattgtct
      721 ttggcgtcaa cgccgtgctc gaggcgctcc gggcgggccg tttattccga cggatcctga
      781 tccagcgggg acgacgcggc ggggacatcg acgacatcga acaggcggct cgcgcggccg
      841 gcgtcccggt tcatgcggag ccccgcgagg cccttgaccg cgccaccggc gtgctcaaac
      901 accagggcgt ggcggcattt ctcaccgaag gcgcgtatcg cgcgttcgac gacatgctcg
      961 cgcacgccgg ccggcagccc ggggggccgt tcctggtcgt gctcgacggg gtggaggacc
     1021 cgcgcaatct cggcgcgatc gcccgcaccg ccgaagccgc gggcgtgcac gggctcatca
     1081 tgccccagcg tcgagcggcc ggcgtgaccg gcgtggccat gaaggcctcg gccggcgcgc
     1141 tcgcgcacct gcccgtggcc caggtcgtga acctcgcgcg cgccatggag cagatgaaaa
     1201 ccgccgggtg ctggctggtg ggtttggacg cgaagggcga ctcggactac accgcggtgg
     1261 attatcgctc gcccctcgcg atcgtggtcg gccatgaagg cgaaggcctg cgcgcctcca
     1321 cgctcaaggc ctgcgacttc cgcgtccgca tccccatgcg cggccgcgtg tcctcgttga
     1381 acgtctcggt ggccgccgcg gtggtgttgt acgaagcgct gcgacagcgg acattacact
     1441 gaccccaagc aacgcttgaa gggcatctct tgcttgtgtt acggtggcgc gactccacac
     1501 acgatttacg ttcggcgctt gaccatgcgc agaccgtgtg cgcaaagact cgctactaaa
     1561 gtttctgatt gagtcggcga gataccaaaa atcttgccaa ttcaggggaa ccactttgaa
     1621 cgaataatgt tcgttctctt gtgattgtga acatgcaaca acaattgagc gaaataaccg
     1681 acactcgtga aaccgtggct cccgttcaat cggcagtcgc tagcccgatc gtaacgcgtg
     1741 aacttgcagc ggtcgaggca ttaaaccgcg aagcggagga gcggtggcga agcaagttgt
     1801 tgctagaagt cgcgctctct cggtcattgg tgagcttcca agctaacaag ggtcgagccg
     1861 tctaccggtg gtttaaatac aaagaaggct tttccgctgg gctgcttgaa tacctcctca
     1921 accgataccg gattaccaat ggcgtgttgc tcgacccgtt cgcgggaagc ggcaccgcgc
     1981 tattcgccgc cggagctttc ggcctccgcg ctgaaggtat cgagttattg cccatagggc
     2041 aaaaaatcat agaaacaaag cagatccttg attggaattt cctgccagag gacgccgagc
     2101 gactgacatg ctggcttcta gaacagcctt ggcggaatca tgtagtggag cagtccctca
     2161 acgagcttcg gatcactcga ggcgcgtatc cgattgaaac cgtttcagcc atgggaaaat
     2221 tcctcga
//
LOCUS       DBNGTT010000143         2437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2073927, whole genome shotgun sequence.
ACCESSION   DBNGTT010000143 DBNGTT010000000
VERSION     DBNGTT010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2073927"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1343
                     /gene="dnaJ"
                     /locus_tag="AC16BC_03270"
     CDS             216..1343
                     /gene="dnaJ"
                     /locus_tag="AC16BC_03270"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011169455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3240669.1"
                     /translation="MAKTRDYYELLGVPRTASEDDLKKAYRKLAMKYHPDRNPGDKAA
                     EAHFKEINEAYSVLSDAEKRQTYDRYGHEAFSAGAGGPGGFGGGFDANVGDIFNDIFE
                     DFFGASTGRSRRRRGAQGSDLKYTLEVTLEDVLTGKDAKIKVPHWENCSACRGTGAKS
                     ATAIATCPACKGAGQIRFQQGFFAVSRTCSQCHGQGKIITEKCPTCHGERRIQHDRTL
                     SVWIPAGVEDGTNLRLAGKGEPGDGGGPSGDLYVVIAVKPHAVFTRDGANLICEAPVS
                     FAKAALGGKLEAATLEGKPVTLKFPAGTPHGKILTLRGQGLPDMHGGGRGDLLVRIRL
                     VVPTKLTPRQRELVEELAKLEGEEVNGEPVGIFAKVKNLFD"
     gene            1346..2122
                     /locus_tag="AC16BC_03275"
     CDS             1346..2122
                     /locus_tag="AC16BC_03275"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR3240670.1"
                     /translation="MPHFFLAPGSIQGDAVTITGTLARHLAGPLRHRAGDRITVVDEG
                     GRRYRIDLVDASTQRIGGRVVESLADEATPALRVTLAHAILKGDHMDVVLQKATEIGV
                     HRIVPLITQRTVVRPKHDRVSRQIERWRAIALEAAQQSERLTIPNVEDAQPLTEWLRS
                     GSGNHPQRWLLGERETRHYFRTELSRHAPATEVTLVVGPEGGFDPEEVGLARAAGFVV
                     VSLGRTILRAETAGVAALTILQYAWGTLGGPQPRAEHAHG"
     gene            2130..>2437
                     /locus_tag="AC16BC_03280"
     CDS             2130..>2437
                     /locus_tag="AC16BC_03280"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3240671.1"
                     /translation="MPDDPTVATEFGQPAGVFRRLVAMLIDWAIVSLLYSGFLVLGVW
                     GASLGARASGAHFLSTDLAETLTGPFILLWLGLTWVYIGWFTRHGGQTPGKRLLGIRI
                     V"
BASE COUNT          457 a          799 c          796 g          385 t
ORIGIN      
        1 ctcccacaag ttggcggagg aaatgtataa gaagaccgcg gcagccggag ctcagccggg
       61 cgccggggcc gccgagggag ccggcgggga cggaaaaccc aaggacgaca acgtggtcga
      121 cgcgcagtac gaggaagtcg acaaagacaa gaagtaacca cctctcaacc ctgcgccggg
      181 cgttcacgtg aacgcccggc gttcggcagg accccgtggc aaaaacccgc gactactacg
      241 aactgctcgg cgtgcctcgc accgcgagcg aggacgacct caagaaagcg taccgcaaac
      301 tcgccatgaa gtaccacccg gaccgcaacc cgggcgacaa ggcggcggaa gcgcacttca
      361 aggaaatcaa cgaagcctac agcgtcttgt ccgacgcgga gaagcgacaa acctacgacc
      421 gctacgggca cgaggcgttc tcggccggcg cgggagggcc cggcggcttc ggaggcgggt
      481 ttgacgcgaa tgtcggcgac attttcaacg acattttcga ggattttttc ggcgcctcca
      541 ccggccgctc gcgccgacga cgcggcgcgc aaggttcgga tctcaaatac acgctcgaag
      601 tcacgctcga agacgttctg accggcaaag acgccaagat caaggtgccg cattgggaaa
      661 actgctcggc gtgccgcgga accggggcca agagcgccac cgccatcgcg acctgcccgg
      721 cctgtaaagg cgccgggcag attcgattcc aacaagggtt cttcgcggtc agccgcacgt
      781 gttcgcagtg ccacggccag ggcaagatca tcaccgagaa gtgtccgact tgccacggcg
      841 aacggcgcat tcagcacgat cgcacgttgt cggtgtggat tcccgccggg gtcgaggacg
      901 gtaccaatct ccgcctggcc ggcaaggggg aaccgggcga tggcggcggg ccgtccggcg
      961 atctctacgt ggtcattgcg gtcaaaccgc acgccgtctt cacgcgtgac ggggccaact
     1021 tgatctgtga agccccggtg tccttcgcca aagccgccct gggcggcaag ctcgaagccg
     1081 ccacgctgga aggaaaaccc gtaacgctca agttcccggc gggaaccccg cacggcaaga
     1141 tcctgaccct gcggggtcag gggctccccg acatgcacgg cggcggccgc ggtgacctcc
     1201 tggtccgcat ccggctcgtg gtgccgacca aactgacccc acgccaacga gagctggtcg
     1261 aggaactggc caaactcgag ggcgaggagg tcaacggcga gcccgttggc attttcgcga
     1321 aggtcaaaaa cctcttcgac tgaccatgcc gcacttcttc ctcgccccgg ggtcgatcca
     1381 gggcgatgcg gtcacgatca ccggcaccct cgcccgtcac ctcgcgggcc cgcttcgcca
     1441 tcgcgcaggc gaccggatca cggtggtgga cgaaggcgga cgccgttatc ggattgacct
     1501 tgtggacgcc tccacgcaac gcattggcgg acgagtggtc gaatcgctgg cggacgaagc
     1561 gacaccagcg cttcgcgtca ccctggccca cgcgatcctc aagggcgatc atatggacgt
     1621 ggtgttgcag aaggccaccg agatcggcgt tcaccggatc gtccccctga ttacccagcg
     1681 caccgtcgtg cgccccaaac acgatcgggt atcgcgccag atcgagcgat ggcgggccat
     1741 tgcgttggaa gcggcccagc agtccgagcg tctcacgatt ccgaacgtgg aggatgcgca
     1801 gcccctgacc gagtggctcc ggtccggatc aggcaatcat ccccagcgat ggctcctcgg
     1861 cgaacgcgag acccggcatt atttccgaac cgagttgtcg cgccacgcgc cagccaccga
     1921 ggtcacactc gtggtcggtc cggaaggagg gttcgaccct gaggaggtgg gccttgcccg
     1981 cgccgcgggt ttcgtcgtgg tctcgctggg tcgtaccatc cttcgcgcgg agaccgccgg
     2041 cgtggcggca ttgacgattc tccagtacgc ctggggaacc ctgggcggcc cccaaccacg
     2101 tgctgagcac gcgcatggtt gacgcagcga tgcctgacga tccgacggtc gcgaccgagt
     2161 tcggccagcc cgcgggcgtg tttcgacgtc ttgtggccat gctcattgat tgggccattg
     2221 tgtcgctgct ctacagcgga ttcctggtgc tcggggtctg gggcgcgtcc ctgggcgcgc
     2281 gagcgtccgg cgcgcacttt ctctccacgg atctcgcgga aacgctgacc ggtccgttca
     2341 ttctgctgtg gctcgggctt acatgggtgt atatcgggtg gttcacccgc cacggcgggc
     2401 agacgcccgg caagaggctt ctggggatcc gaatcgt
//
LOCUS       DBNGTT010000144         6048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3479245, whole genome shotgun sequence.
ACCESSION   DBNGTT010000144 DBNGTT010000000
VERSION     DBNGTT010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3479245"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="AC16BC_03285"
     CDS             <1..1481
                     /locus_tag="AC16BC_03285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3240672.1"
                     /translation="FAPTRRLLAWVAGIGLVAIGLLGTLGYVAAIRIVRPIRRLQQGA
                     ALIGRNELKEPIVVRTGDEIQGLAEEINRMNSRLQDAFSGLNQEVRSLQAYNEQILNS
                     VPNPIIMLDHERVQYVNRAARRAFGLNDRCERGLGLFDVVAVDERSRERLRGEIRACD
                     ASRPWSNEDADVSSSSTSPGDPLGPRRPADADPGGKELTINRVTYRYEIFRIDTPTTD
                     AGWTGLVLWDTTLDSQRQAQLIQAEKLAGLGVLTSGIGHELNNPLFGILSLSEAIRDE
                     RDPARVRTHANEILSIAKRMARIIRDFAGSSRSDQTELRDDVNVNEQLDLALRVVGLI
                     HEAAGLHVRRQYQPVRPIRAVPGEIGQVFVSVITNAIQAMKGKGVIDLCTETSADAVT
                     VTIRDQGPGVANAHASRIFDPFFTTKAPGQGTGLGLTMARRILMRCGGQIRLDNPGGP
                     GATFVLTFPVSQPEPPDSGDVTTHVHLNPPVSTIRGGQVFNR"
     gene            complement(1536..1868)
                     /locus_tag="AC16BC_03290"
     CDS             complement(1536..1868)
                     /locus_tag="AC16BC_03290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3240673.1"
                     /translation="MTGETSRHVYRVDQFVVPDSAREEFLGKVRGTHRLLKAQPGFVR
                     DVVLEQTAGPGRFNFVTLVEWRSQSAMDAAKAVVTAAHAKSGFNPQELFARLGIQADI
                     ANYTAIDA"
     gene            complement(1870..2304)
                     /locus_tag="AC16BC_03295"
     CDS             complement(1870..2304)
                     /locus_tag="AC16BC_03295"
                     /inference="COORDINATES: protein motif:HMM:NF022592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HIR3240674.1"
                     /translation="MNARRPSENVLGRPRSGSAEPRVRIGPARQAGRESLFQRLAAWL
                     AGMTVRMRSRRSASQAREAVVRLDSNQVWTHRVPRGLDLSVTCVRGQVWMTREGDSRD
                     HFLRGEDTYSSREPGLVCVQAIAPAQLLVTYKRSSRSIVRVR"
     gene            complement(2282..3010)
                     /locus_tag="AC16BC_03300"
     CDS             complement(2282..3010)
                     /locus_tag="AC16BC_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240675.1"
                     /translation="MNVDLLASIEMTACAAVAIAVLAIGFGEDVSTRIRLATGLAAWF
                     MLVTAMAATEVLHDQRGFGVPGLGVAVILPLVILSVGVFRSPTLYQALRAIPLSALIV
                     VNVIRVFGVMFLLLYAAGRLPAPFAPVAGWGDILVGLFAAPVAWLAHKKGATAASRVR
                     IWNTIGLLDLIAAVGLGVASSSGPLQPIFTEPGSGIMTTLPWLLIPGFLVPLLASTHL
                     AVFYRLSTAGERRNHRTHERPATV"
     gene            complement(3026..4480)
                     /locus_tag="AC16BC_03305"
     CDS             complement(3026..4480)
                     /locus_tag="AC16BC_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR3240676.1"
                     /translation="MIVHRVAIAALIATLLTGCFTLGPDYRRQAIDTPDAWPTPTAAA
                     SIPDEWWRGYGDPALDTMIQEALGHNADLRLAIARVDEARASLGVARADQWPGVSANA
                     AASRNRVSEDSALALPGVDPIYNNVRGTLDASYEIDFWGKYRRATEAARAELLATEFS
                     RDAVRLALIADVSRGYFNLRALDAQVAITKRTVSTRVDSLALQRQRFAAGVASELELR
                     QVEAEAAAAQALLPALENQLARQETALSVLLGRSPREIVGSTPERGAAIEALTVPHAV
                     PAGLPSDLLEHRPDLRQAEQQLIAANARIGQAKAAYFPSITLTGLLGGESTKLVDLFS
                     APARIWQASGSAAQLVFDAGRTGSRVNAAKALEQQALARYQSAIQNAFRDTLDALVAQ
                     RKARETLDAEQARVAALQGALDLARLRYQNGVSSQLDVLDAERGLLAAELNRVEAQRA
                     QLAATADLFKALGGGWRSDGQSQQRTASATGAGG"
     gene            complement(4477..>6048)
                     /locus_tag="AC16BC_03310"
     CDS             complement(4477..>6048)
                     /locus_tag="AC16BC_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008219380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3240677.1"
                     /translation="VGMVVITAGLWRATPGSLVPDEDQGYYIGAVFLPDGASLQRTDK
                     VVDEVVRAIRSNPANEYAIAFTGLDFLGGGFRNSAATIFVTQKHWDERQVYTPQLVGE
                     FFMKTAHIKEALVLAFGPPPIFGLGNSGGFEFYVQNRGEGGPQRLAQVMGEFLAAANQ
                     EPMLAGAQTLWRSNVPQLRVDVDREKAKALGVPINDLFDTLSATLGSYYVNDFNRFGR
                     TWQVLMSAEPSYRSTPDSVGELYVRSDQGAMVPVKALATVSYTAGPETLDRFNNLPAV
                     KILGQAAPGVSSGQAIQTVERVAEKVLPTDFSFDWGGASFQEKRSSGTSGLALGLAVI
                     MVFLILAAQYGKWSLPISVLLALPFGTFGALAAVWLRGLTNDVYFQIGLVTLLGLAAK
                     NAILIVEYAVLKHREGLPPADAALEAARLRFRPILMTSLAFILGVAPLAFSTGAGAGA
                     RISVGTGVMGGMLAATFLAIFFVPMFFKLIADRRSEGKRGTPGVAIDVEYQKAMVGGN
                     GAAAPAALTPQGGRS"
BASE COUNT         1065 a         2084 c         1954 g          945 t
ORIGIN      
        1 tgttcgcgcc gacgcgccgg ctgctggcgt gggtcgcggg tatcgggctg gtcgccatcg
       61 gcctgctcgg caccctcgga tacgtcgccg cgattcgcat cgtgcggccc atccggcgcc
      121 tgcaacaagg cgccgcgttg atcggtcgga acgaattgaa ggagccgatc gtggtccgca
      181 ccggcgacga gatccaggga ttggccgagg agatcaaccg gatgaacagt cggttgcagg
      241 acgccttttc cggtctgaac caggaagtgc gttccctcca ggcctacaac gagcagattc
      301 tgaacagcgt gcccaacccg atcatcatgc tggaccacga acgcgtgcag tacgtcaacc
      361 gcgcggcgcg ccgcgcgttc ggcttgaacg atcggtgtga gcgcgggctg gggctgtttg
      421 acgtggtcgc ggtggacgag cgctcgcgcg agcggttgcg cggagagatc cgcgcctgcg
      481 acgcgagccg gccgtggtcc aacgaggacg ccgacgtctc gtcctcgtca acatcgccgg
      541 gtgacccctt ggggcctcga cgcccggcgg acgctgatcc gggcggcaag gaattgacga
      601 tcaaccgcgt gacctatcgg tacgaaatct tccggatcga cacgccgacc accgacgcgg
      661 ggtggaccgg actcgtgttg tgggacacga ccttggacag ccagcggcag gcccagttga
      721 ttcaagccga gaaactcgcc gggctgggcg tcctgacgtc gggcatcggc cacgaactca
      781 acaacccgct gttcggaatc ctgagcttga gcgaagcgat tcgcgacgag cgggaccccg
      841 cgcgcgtcag gacccacgcg aacgagatcc tgtccatcgc gaaacggatg gcccgcatca
      901 tccgggactt cgcggggtcg tcgcggagcg accagaccga actgcgggac gacgtgaacg
      961 tgaacgagca actcgacctg gcgttgcggg tggtcggatt aatccatgaa gcggcggggc
     1021 tccacgtgcg ccgccagtac cagccggtcc gaccgatccg ggcggtgccc ggcgaaatcg
     1081 ggcaggtatt tgtcagcgtg atcaccaacg ccatccaggc catgaagggc aagggcgtga
     1141 tcgatctctg caccgagacc tccgcggacg cggtcacggt cacgatccga gaccagggtc
     1201 cgggcgttgc gaacgctcac gcctcgagaa ttttcgatcc gttttttacc accaaggcac
     1261 cgggccaggg gaccggcctg ggcctgacca tggcccgccg gattctcatg cgctgcggcg
     1321 gacagatccg gctcgacaat ccgggcggcc ccggcgcgac ctttgtgttg acgttcccgg
     1381 tgtcgcagcc cgagccgccg gactcgggcg acgtgacaac ccatgtccac ctcaatccgc
     1441 ccgtgagtac catccgagga gggcaagtct tcaatcggta gttcggttct ctccgatacg
     1501 cgactcatac gccggcggcc ggtcaaggaa acgggtcagg cgtcaatggc cgtgtagttc
     1561 gcaatatccg cttggattcc cagccgcgcg aacagttctt gcggattgaa gccgctcttg
     1621 gcgtgggcgg ccgtcaccac ggccttggca gcgtccatgg cggattggct ccgccactcc
     1681 accagcgtga cgaagttgaa tctgcccgga ccggctgttt gttcgagcac gacgtctcgc
     1741 acaaaccctg gttgtgcctt caagagccgg tgcgttccgc gcactttgcc gaggaactcc
     1801 tcacgcgccg agtcgggcac cacgaactga tcgacacggt acacatgccg cgatgtctca
     1861 cccgtcatgt catcgcaccc ggacgattga tcgagaggaa cgcttgtacg tcacgagcaa
     1921 ctgcgccggg gcgatcgcct gcacgcacac cagcccgggt tcgcgactgc tgtacgtgtc
     1981 ctcgccgcgc agaaaatggt cacgcgagtc tccctcgcgg gtcatccaca cctgaccgcg
     2041 cacgcacgtc acgctgaggt ccaaaccccg cggtactcga tgcgtccaaa cctggttgga
     2101 gtcgagccgc accactgcct cgcgggcttg agacgctgat cgtcgcgaac gcatccggac
     2161 agtcattccc gccaaccacg cagcgagccg ctggaagagc gactctcggc ccgcctgccg
     2221 cgccggccca atgcgaacgc ggggctccgc cgatccggac cgcgggcgac ccaaaacgtt
     2281 ttcagacggt cgccgggcgt tcatgggttc ggtggttgcg gcgctccccc gccgtgctca
     2341 ggcggtagaa caccgcgaga tgcgtggacg ccagcagcgg gaccaggaaa cccgggatca
     2401 gcagccacgg cagcgtggtc atgatgcctg atcccggctc ggtgaagatc ggttggagcg
     2461 gaccggacga cgaggccacg cccagtccca ccgccgcgat cagatcgagc agtccgatgg
     2521 tattccagat ccgcaccctc gaagcagcgg tcgccccctt cttgtgcgcc agccacgcga
     2581 ccggcgccgc aaagaggccg accaggatat cgccccagcc cgccaccggc gcaaagggcg
     2641 cgggcaagcg gcccgcggca tacagcagca ggaacatcac gccgaagacg cggatcacgt
     2701 tgaccacgat gagcgcggac aacgggatcg cgcgcagggc ttggtataac gtcggcgagc
     2761 gaaacacgcc gacactgagg atcacgagcg ggaggatcac cgcgactccg aggcccggca
     2821 ccccgaaccc acgctgatcg tgcagcactt cggtggccgc catcgccgtg accagcatga
     2881 accacgcggc gagcccggtc gcaagacgaa tgcgcgttga gacgtcttcc ccgaatccga
     2941 tggccaacac cgcaatggcc acggccgcgc aggccgtcat ttcaatcgac gcaagcagat
     3001 cgacgttcat gccccacctc cggtctcaac cacccgcacc cgtcgcggat gcggtccgtt
     3061 gttgcgattg gccatcactc cgccaccccc cgcccaacgc cttgaacagg tccgcggtgg
     3121 ccgcgagttg cgcgcgttgc gcctccacac ggttgagttc cgcggccagc aacccgcgct
     3181 ccgcgtccag cacgtcgagc tggctcgaca cgccgttttg gtagcgcagc cgcgccagat
     3241 ccagcgcgcc ctggagcgcg gcaacccgcg cctgctccgc gtccagcgtc tcgcgcgcct
     3301 tgcgctgcgc caccagggcg tcgagcgtgt cccgaaacgc gttttggatc gcggactggt
     3361 accgcgcgag cgcttgctgc tccagcgcct tcgcggcgtt gacccgcgac ccggtgcggc
     3421 ccgcgtcgaa caccagttgc gcggctgatc ccgaggcctg ccagatccgg gcgggcgcgc
     3481 tgaacaggtc aaccagcttg gtgctctcgc cccccagcaa gccggtcagg gtgatcgacg
     3541 gaaaatacgc ggccttggcc tggccgatcc gggcgttggc cgcgatcaac tgctgctccg
     3601 cctgtcgaag gtccggccgg tgctccagga gatccgacgg caacccggcg ggcaccgcgt
     3661 gaggcaccgt gagcgcctcg atcgcggcgc cccgctccgg ggtggagccc acgatctcgc
     3721 gcgggctgcg acccagtaac acggaaagcg cggtttcctg ccgcgcgagt tgattctcga
     3781 gcgcgggcag taaggcctgc gccgccgcgg cctcggcttc cacctgccgc agttcgagtt
     3841 cggacgccac gccggccgcg aaccgctgtc gttgcaaggc cagcgagtcc accctggtgg
     3901 agaccgtacg cttggtgatc gccacctgcg catcgagcgc gcgaagattg aaatagcccc
     3961 gcgacacgtc cgcgatcaat gccaggcgca ccgcgtcgcg actgaactcg gtggcgagca
     4021 gctcggcccg cgcggcttcg gtggcgcggc ggtacttgcc ccagaagtcg atttcatacg
     4081 acgcgtccaa cgtgccgcgc acgttgttgt aaatcgggtc cacgcccggc agcgccaagg
     4141 cgctgtcttc gctcacccgg tttctcgacg cggcggcatt ggccgacacg cccggccact
     4201 ggtcggctcg cgccacgccc agactcgcgc gcgcctcgtc cacccgcgcg atggcgagcc
     4261 gcaggtccgc gttgtggccg agcgcttcct ggatcatggt atcgagcgcc ggatcaccgt
     4321 agccgcgcca ccattcgtct ggaatcgacg ctgcggcggt cggcgtgggc caggcgtccg
     4381 gcgtgtcgat ggcctgccga cggtaatccg ggcccagagt gaagcagccc gtgagcagcg
     4441 tggcaatcaa cgcagcgatg gcaacacgat gcactatcat gatcgtcctc cttgcggcgt
     4501 cagcgcggca ggggccgcgg cgccgtttcc cccgaccatg gctttttggt attcgacgtc
     4561 gatggcgacg cccggggtcc cacgcttccc ctcggagcgc cggtccgcga tgagcttaaa
     4621 gaacatgggt acgaagaaaa tggccaggaa cgtggcggcc agcatgccgc ccatcacgcc
     4681 ggtgccgacc gagatacgcg cccccgcgcc cgcaccggtg gagaacgcga gcggcgcgac
     4741 ccccaagatg aacgccagcg aggtcatcag gatcgggcga aagcgcagtc ttgcggcctc
     4801 caacgcggca tccgcaggag gcaacccctc gcggtgcttc aagaccgcgt attcgacgat
     4861 caggatcgcg ttcttggcgg ccagccccag taacgtcacc aagccgatct ggaagtacac
     4921 gtcgttggtc aagccacgca accacaccgc ggccaatgcg ccgaacgtgc cgaagggcaa
     4981 tgccagcagc accgagatcg gcagcgacca tttcccatac tgcgcggcca ggatcagaaa
     5041 caccatgatc accgccagcc cgagcgcaag gcccgacgtg ccgctggatc gcttttcctg
     5101 gaacgatgcg ccgccccagt caaagctgaa atccgtgggc agcaccttct ccgcgacccg
     5161 ctcaacggtc tgaatggcct ggcccgagga cacgccgggc gcggcttgcc ccaggatctt
     5221 gaccgcgggc aggttgttga accggtcgag cgtctcgggc ccggcggtgt agctgaccgt
     5281 ggccagggct ttgaccggca ccatcgcgcc ctggtcggat cgcacgtaga gctcgccgac
     5341 cgagtcgggc gtgcttcgat aggagggttc cgcggacatc aacacctgcc aggtccggcc
     5401 gaatcggttg aagtcattga cgtagtacga gcccagggtc gcggacaacg tgtcgaacag
     5461 atcgttgatc ggcaccccga gggctttggc tttttcgcga tccacgtcca cgcgcagttg
     5521 cggcacgttt gagcgccaca gggtctgcgc gcccgcgagc atgggctcct ggttggccgc
     5581 ggccagaaac tcgcccatca cctgggccag gcgctgcggc ccgccttcgc cgcggttctg
     5641 tacgtagaac tcaaacccgc cggagttccc cagcccgaag atcgggggcg ggccgaaggc
     5701 gagcaccaag gcttctttga tgtgcgcggt cttcatgaag aactcgccca ccagttgcgg
     5761 ggtgtagacc tggcgctcgt cccagtgctt ctgggtcacg aaaatggtgg ccgcgctgtt
     5821 gcggaacccg ccgccaagaa aatccaatcc ggtaaaggcg atcgcgtatt cattggccgg
     5881 gttggagcga atggcccgca ccacctcgtc gaccaccttg tccgtgcgct gcagggacgc
     5941 gccgtccggg agaaagaccg cgccgatgta gtagccctgg tcctcgtccg gtaccaggct
     6001 ccccggcgtg gcgcgccaga gtccggccgt gatcaccacc atgccgac
//
LOCUS       DBNGTT010000145         5140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1946633, whole genome shotgun sequence.
ACCESSION   DBNGTT010000145 DBNGTT010000000
VERSION     DBNGTT010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1946633"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC16BC_03315"
     CDS             <1..183
                     /locus_tag="AC16BC_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240678.1"
                     /translation="HARALLLDNVCRDNDHWGVVITPDSETTPPRDELIAANSFASNP
                     RGAMTVTDAPLADIGR"
     gene            303..1040
                     /locus_tag="AC16BC_03320"
     CDS             303..1040
                     /locus_tag="AC16BC_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240679.1"
                     /translation="MSEPVIEVEGVGKRFKDVWAVADLSFTVGRAEIVGLLGPNGAGK
                     TTTLAMLLALLVPTAGRIRLFGLDVASHRQAVLGRMNFSSPYADLPHRLTVRENLSVY
                     GRLYGVHRLRDTSTELGQRLDLAALMDRRFGELSSGQRTRVAIAKALINAPELLLLDE
                     PTASLDPDIADRVRAWLADYRTQTGAAILLSSHNMAEVERLCDRVVVLQHGRCVAQDT
                     PARLVAQYGRRSLEDVFLAITRKEHDP"
     gene            1037..1828
                     /locus_tag="AC16BC_03325"
     CDS             1037..1828
                     /locus_tag="AC16BC_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3240680.1"
                     /translation="MNPHRVLAMVRRHVYLYRSSWTRLVELVYWPAMELFVWGFLTIY
                     LRGLGPGLPDFVVLFLGGLILWDVLFRSQIGVSLAFLEEIWSRNLSNLFASPLTPLEF
                     VASQIIVALARTVTASLFMALLAALLYHYNLFTMGLPLAAFFVNLLIMGWSIGLLVSA
                     LILRFGQGAESLAWAVIFLFQPVAAVFYPVAVLPPWLQPIALATPAAHVFEGMRALLL
                     DGRFDVGHIIGAFVLNIVYLVLGCAGFLGMFRVARRKGLLLQVGE"
     gene            1851..2156
                     /locus_tag="AC16BC_03330"
     CDS             1851..2156
                     /locus_tag="AC16BC_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240681.1"
                     /translation="MTIGEIEEFLWSLSTCLEGEIGNWRLRFGGAWVSVRADPDADEV
                     VISAAVSGHESIARRGSPASATTTARLSVLTINALQAAFQHVVDLAHQTARSDPPEP"
     gene            2153..3736
                     /locus_tag="AC16BC_03335"
     CDS             2153..3736
                     /locus_tag="AC16BC_03335"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR3240682.1"
                     /translation="MTPAVIRRQYAALLALLGIAIVAGCAAWTRSDETRTFTIVQLND
                     VYDVFPVSVEANGHTELRGGLAYAATMIREERRRGPLLVLHAGDLLGPSVLSTALKHR
                     GAQMIEALNALGVDAATFGNHEFDDRCRVLAQRIHESRFLWLSANVAFPPEAELAADR
                     VARRRIVVVNGLRVGLFGLSLPMSPVPGCGDGVITFHDPIEAAREAVDALTRDGADVI
                     IAVTHLPMAVDRALAEALPAIDLIVGGHEHEPMDAKIGRTLITKAGANAEALGVITVT
                     VAKTRGKPEINARWTRRAVDPASVAPDPEMTRVLAPYARELEPFAAVIGRSAVPLDAR
                     EEVVREAESNLGNYVADAVREAMRADAALINGGAFRDDRIIPAGPLTLGDVHTVLPFV
                     NQLVTVRVTGAELLAALENGVSLAGHKAGRFPQVSGVRFAFTPHAAPGQRVIHARVND
                     APVEPERAYTLATTDFLIRKNTMDGYTFPENAQERGGLLTEVVLERIRMGSPIQPRVE
                     GRIERVEAGPPTAPGSMRP"
     gene            complement(3737..4015)
                     /locus_tag="AC16BC_03340"
     CDS             complement(3737..4015)
                     /locus_tag="AC16BC_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIR3240683.1"
                     /translation="MTKAELIEDVSQKVKGLTKRQTEIIINAILDGIKETLARGDKIE
                     IRGFGSFRLRSRRTREGRNPKTGTAVHVPAKRVPFFKAGKELKELVDR"
     gene            complement(4032..4916)
                     /gene="sppA"
                     /locus_tag="AC16BC_03345"
     CDS             complement(4032..4916)
                     /gene="sppA"
                     /locus_tag="AC16BC_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3240684.1"
                     /translation="MARRPLLTGFMVFLILAVAFFLGTYLTARLVERPAGAANQIALV
                     RVEGVILSADEAVEQLRDFAENEAIRAILIRIDSPGGAVVPSQEIYDQVKKIRAEGRQ
                     KVVVSMGSVAASGGYYIASASDRIVANPGTLTGSIGVILEMPNFEELMKKIGVENTVV
                     KSGAHKDLISPFRKLGDEERAILQRVMDDVHDQFIQAVSDGRGLAKDTVAAIADGQVF
                     TGRQAKDKGLVDEIGSFEDAITITGELAGIVGRPVVVEPKRPFSLTDLIRTAFRGRLG
                     ALVDPASTIRLNYLWTWG"
BASE COUNT          800 a         1750 c         1669 g          921 t
ORIGIN      
        1 cacgcccggg ccctgctgct ggacaacgtg tgccgcgaca acgaccactg gggagtcgtg
       61 atcacgcccg actccgagac caccccgccg cgtgacgaac tgatcgcggc caactcgttc
      121 gcgtcgaatc cgcggggcgc catgacggtc acggacgccc ccctggccga catcggtcgg
      181 tagcgcggtg gatgtactca ccttccacgt aatcgccgca acgctccggc gccgggcgcg
      241 atacctccgt ccctcctttg aaaaaggggg acgagggggg atttgctttt gacccgctct
      301 ccatgagcga acccgttatt gaagtcgagg gcgtgggcaa acggttcaaa gacgtctggg
      361 ccgtcgcgga cctgtcgttc accgtggggc gcgcggaaat cgtgggactg ctcggcccga
      421 acggagccgg caagaccacc acgctcgcca tgctgctcgc gctgctcgtc cccaccgccg
      481 ggcggatccg actgttcgga ctggacgtgg cctcgcatcg gcaggccgtg ctgggccgga
      541 tgaacttttc gtcgccctac gccgacctgc cgcaccggct gaccgtgcgc gagaacctgt
      601 cggtctacgg ccgcttatac ggcgtccacc gactccgcga caccagcacg gaattgggac
      661 aacggctgga cctcgcggcg ctgatggatc gtcgcttcgg agaactgtca tccggccagc
      721 gcacgcgcgt ggcgatcgcc aaggcgttga tcaacgcccc cgaactcctg ttgctggatg
      781 aaccgaccgc gagcctggac ccggatatcg cggaccgcgt gcgggcgtgg ctggcggact
      841 atcggaccca aaccggcgcc gccatcctct tatcctcgca caacatggcg gaagtggagc
      901 ggctgtgcga ccgggtcgtg gtcctgcaac acggtcgctg cgtggcccag gacacgcccg
      961 cccggctggt cgcgcaatac ggtcgccgat cgcttgaaga cgtatttctc gccatcaccc
     1021 gcaaggagca cgacccgtga atccgcaccg cgttctggcc atggtgcggc gtcacgtcta
     1081 cctctaccgc agctcgtgga cccgcctggt cgaactcgtc tactggcccg cgatggagtt
     1141 gttcgtgtgg ggctttctca cgatctacct ccgggggctc gggcccggac ttccggactt
     1201 cgtggtgctc ttcctcggcg gcctgatctt gtgggacgtg ctgtttcgtt cgcagatcgg
     1261 cgtgtcgctc gcgttcctgg aggagatctg gtcgcgcaac ctgtcgaacc tgttcgccag
     1321 ccccctgacc ccgctggagt tcgtcgccag tcagatcatc gtcgcgctcg cccgtaccgt
     1381 caccgcgtcg ctgttcatgg cgctgctggc ggcgctgttg tatcactaca acctgttcac
     1441 gatggggttg ccgttggccg cgttcttcgt caacctgctg atcatgggct ggtccatcgg
     1501 cctgctggtg tccgcgttga tcctgcgatt cgggcaaggc gccgagagtc tggcctgggc
     1561 cgtgatcttt ctgttccagc cggtcgcggc cgtgttctac cccgtcgcgg tgctcccgcc
     1621 gtggctgcag ccgatcgcgc tggccacgcc ggccgcccac gtgttcgagg gcatgcgcgc
     1681 attgttgctg gatggacgct tcgacgtcgg ccacatcatc ggcgcattcg tcctcaacat
     1741 cgtgtatctg gtcctggggt gcgcgggatt tctcggcatg ttccgcgtcg cgcgccgcaa
     1801 ggggctgtta ctgcaggtcg gggaatgatc gatccgggtg cgcaccgtcg atgaccatcg
     1861 gcgaaatcga ggagtttctg tggagcctct ccacctgtct ggagggcgag atcggaaact
     1921 ggcgtcttcg gttcggcggc gcctgggtgt cggtccgggc ggatccggac gcggatgagg
     1981 tggtgatcag cgccgccgtc agcggccacg agtctatcgc gcgccgcgga tcgcccgcgt
     2041 cagccacgac caccgcgcgg ctctcggtgc tgaccatcaa cgcgctccag gccgcgtttc
     2101 agcacgtcgt cgatctggcg caccagaccg ctcgctccga cccacccgaa ccatgactcc
     2161 cgcggtgatt cgtcgccagt acgcggcgct gctcgccctg ctcgggatcg cgattgttgc
     2221 gggctgcgcc gcctggaccc ggtcggatga aacccggaca ttcaccatcg tgcagctcaa
     2281 cgacgtgtac gacgtcttcc cggtgtcggt cgaggctaac gggcataccg aactgcgcgg
     2341 cggactggcg tacgcggcca ccatgatccg cgaagaacgg aggcgcggcc ccttgctggt
     2401 gctgcacgcc ggcgatttgc tcgggccgtc cgtgttgtcc accgcgctca aacaccgcgg
     2461 cgcccagatg atcgaggcgc tcaatgcgct gggcgtggac gccgccacgt tcggcaatca
     2521 cgaattcgac gaccgctgcc gcgtgctggc gcaacggatt catgaatcgc gatttctctg
     2581 gctgtccgcg aacgtcgcat tcccgcccga ggccgaactc gcggccgatc gcgtggcgcg
     2641 ccgccggatc gtcgttgtca acggtcttcg cgtcggcttg ttcggactct ccctgcccat
     2701 gagtccggtt cccggatgcg gagacggggt cattacgttc catgatccga tcgaggcggc
     2761 gcgggaagcc gtggatgccc tgacccgcga cggcgcggac gtgatcatcg cggtgacgca
     2821 ccttccgatg gccgtggacc gcgccctggc cgaagccctg cccgcgatcg atctgatcgt
     2881 gggcgggcac gagcacgagc cgatggacgc gaagatcggc cgcacgttga tcaccaaggc
     2941 cggcgccaat gccgaagccc tcggcgtgat cacggtcacc gttgcgaaga cgcgcgggaa
     3001 gccggagatc aacgcgcggt ggacacggcg cgcggttgat cccgcctccg tggcgccgga
     3061 ccccgaaatg acacgggtgc tcgcgccgta cgcgcgggaa ctggagccgt tcgccgcggt
     3121 gatcggccgc agcgcggtcc cgttggacgc gcgcgaggag gtggtgcggg aagccgagag
     3181 caatctggga aactacgtgg ccgacgcggt gcgcgaggcc atgcgcgccg acgcggcgct
     3241 gatcaacggc ggcgcgttcc gggacgaccg catcatcccg gcgggccccc tcaccctggg
     3301 cgacgttcac accgtgctgc cgttcgtcaa tcaactcgtc accgtgcgcg tgaccggcgc
     3361 ggagctgctg gccgcgctgg agaacggcgt gagcctcgcc ggtcacaagg ccggacggtt
     3421 tccccaggtc tccggggtgc ggttcgcgtt caccccccac gcggcgcccg gacaacgcgt
     3481 gattcacgcc cgggtcaacg acgcgcccgt ggagccggag cgcgcctaca ccctggcgac
     3541 cacggacttt ctcatccgga aaaacacgat ggacggctac acgttcccgg agaacgcgca
     3601 ggaacgcggg ggattgctga ccgaggtggt gctggagcgg atccgaatgg gatctcccat
     3661 ccagccccgc gtggaaggca ggatcgagcg cgtggaagcg gggccgccaa ccgcgcccgg
     3721 ctcgatgcga ccgtgatcag cggtcgacga gttctttgag ttctttcccg gccttgaaaa
     3781 acggcacgcg tttggccggt acgtgcaccg cggtcccggt cttggggttt cggccctcgc
     3841 gcgtgcggcg gctgcgcagg cggaagctgc cgaatccgcg gatctcgatt ttgtcgccgc
     3901 gcgccagggt ctccttgatg ccgtcgagaa tggcgttgat gatgatctcg gtctgacgtt
     3961 tggtcaggcc tttgaccttt tgcgaaacat cctcgatcaa ctcggctttg gtcatcgcgt
     4021 gcgctccccg actatcccca ggtccacaga tagttcaacc ggatggtcga agccgggtcc
     4081 accaacgccc cgagtcgacc gcgaaatgcc gtgcggatca gatcggtcag cgaaaacggc
     4141 cgcttcggtt ccacgaccac gggacgcccc acgatcccgg ccagttctcc ggtgatcgtg
     4201 atcgcgtcct cgaagctgcc gatctcgtcg accaatccct tgtctttggc ctgccgcccg
     4261 gtaaacacct ggccgtcggc gatggccgcc accgtgtcct tggccaggcc ccggccgtcc
     4321 gacacggctt gaatgaactg atcgtgcacg tcgtccatca cgcgctggag aatcgcccgc
     4381 tcctcgtcgc ccagcttgcg aaacggcgag atcaggtcct tgtgcgcgcc gctcttgacc
     4441 accgtgtttt ccacgccgat cttcttcatg agctcttcga agttcggcat ctcgaggatc
     4501 acgccgatgc tgccggtcag ggtgccgggg ttggccacga tgcggtccga cgcgctcgcg
     4561 atgtagtacc cgccggacgc cgcgaccgac cccatcgaca ccacgacctt ctgccgccct
     4621 tcggcgcgaa tcttcttgac ctgatcgtag atttcctggg acgggaccac ggccccgccg
     4681 gggctgtcga tccggatcaa aatggcgcgg atggcctcgt tctccgcgaa atcgcggagt
     4741 tgctccacgg cttcatccgc gctcagaatc acgccctcga cccgcaccag cgcgatctga
     4801 ttggccgcgc ccgccggccg ctccaccagc cgcgcggtga ggtacgtccc gagaaaaaac
     4861 gccacggcca aaatcaggaa caccatgaac ccggtcagca gcggtcgtct ggccatacga
     4921 gtggtcggtt cggactcttt aatatgccag ttagcccact accaaagccg gttcaggggt
     4981 ggtcagatgc aaggcgccgc gagcaccgga gcggagcgta cgtggtgagt acgtgagcac
     5041 cggaagcgca gcggcaacgc cgcagacggc cgttcatgaa ccgactaacg gcgcttagcg
     5101 gtgtggcggt cgccgccctc cgagagcccc cagtcgccca
//
LOCUS       DBNGTT010000146         4235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_924443, whole genome shotgun sequence.
ACCESSION   DBNGTT010000146 DBNGTT010000000
VERSION     DBNGTT010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_924443"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="AC16BC_03350"
     CDS             complement(<1..823)
                     /locus_tag="AC16BC_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3240685.1"
                     /translation="MRVLLVTPPLTQLNTPYPATAYLAGFLASRGIDAAQADLSLETA
                     LAVFSRSGLTDLFARLRERGPEWAATSERALALAEAYLATIDPVVAFLQGRDPTLAVR
                     IADGAFLPEGPRLAAREDDRWAFGAMGTADRAKHLASLYLDDLSDLITATVDPLWGLS
                     RYAERLAASAASFDPLEKALREPPGFAAAFLESRAREMARGPRPDLVAITVPFPGNLY
                     GALRTASVLRSEWSGVPIVLGGGYLNTELRELDEPRLFDYVDYVTLDAGERPLLCL"
     gene            complement(869..>4235)
                     /locus_tag="AC16BC_03355"
     CDS             complement(869..>4235)
                     /locus_tag="AC16BC_03355"
                     /inference="COORDINATES: protein motif:HMM:NF051835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR3240686.1"
                     /translation="FTARVVGGSTSEVTWSVEPAGGGTITSDGLYTAPAQTGTFTVAA
                     ETKAAPITRKTVFVTVVDQPVPGVEIDDLSADPAKPMVNGETRLFQARLTNASLVEVF
                     WGVTPVDPSATANSLPMPSVTPTGSYTQFTASDRPGIYRLRAEVRDRATGDFIVATDR
                     LVDAITVGVCVYTGSPPLATDGSVAPCDGTTWATRTVTLPAKGTVTFGATVTTNDGIL
                     QPRTYTAPTPGLITWTWAVQDLSGGDIVATSGAYTAPVLGGTYHVAATLDQYSKSGSA
                     TVAVNRVKVAVGPQSASVRAGDTLDLTATVTDVVNDYGDVLWSIQEGSVGGTLGAAVF
                     TPTSPTSSTSQVTYRAPAGGGGRVFHIVATSASDRAVRATATVTVLGVWLSAAAGTHH
                     TVAVKSDGTLWAWGNNYYGQLGDGTTVNRPSPVQIGTATDWDSVAAGADFTLALKRNG
                     TLYAWGANAAGQLGDGTFTDRNVPVKIGADFEWHAVSAGGRHTAGIKINGTLWTWGDN
                     TYGEAGNNTSGYIIPTPWPVTDATGVPLAMQWSAVAAGTDHTLAIATDGSLWGWGRNT
                     AGQVGDTAVPGGQPVDRLVPTRVGIETDWIAIDAGGSHSAGVRRTALGAAAGTLWVWG
                     DNADGQLGVPAVTGALAPMQVGTGTDWASVVLGETDTLAITGDQTLWGWGNNAYGQLG
                     LGESAPDLVNEPTLIGTGWFAAAAGYFHSVRLTANGRLFVTGGNLYGEIGNGAVATKY
                     VPVRAGGTSVDWSGATVAAGGFHTAAVKADGSLWTWGSNTLGQLGNGTPYDSETPARV
                     GTETWTHVATGAFHTAAIRSDGRLFTWGDNSSGQLGVGIQVDQSSVPMEVPEFLAFDP
                     WVAVSAGGYHTVAVTRQGKLFAWGNNAVGQVGASTGGSSIEYLPVRLIPTSDSWHPAV
                     AGGYHTLGVKKHVVDGIASFSLWAWGSNASGQLGDGSVTDQFAPVEITSALMNAATLP
                     DGGIKIAAGAQHTVAVNPNGELWVWGNNFFGQVGLIGATSSSMPIRLDAATNWALPAA
                     GFGHTVAIKTDGSLWTWGRNTLGQLGNGTTSARDVPGQIGLGASWSTVSTFGDHTVAV
                     RADGTLWAWGANDSGQLGDGSAWVDTPVEVAAP"
BASE COUNT          677 a         1524 c         1365 g          669 t
ORIGIN      
        1 gcagacagag caacgggcgt tcacccgcgt ccaacgtgac ataatccacg tagtcgaaca
       61 gacgcggctc gtccaactcg cggagttcgg tgttgaggta cccgccgccg agcacgatgg
      121 gcacgcccga ccactcactc cgcagcaccg acgcggtacg caatgcgccg tacaggttgc
      181 cggggaacgg cacggtgatc gccaccaggt cgggacgcgg ccctcgggcc atttcccgcg
      241 cccgcgactc caggaaggcg gccgcgaacc ccggcggctc ccggagcgcc ttctccaaag
      301 ggtcgaacga tgcggcggag gcggccaggc gttcggcgta gcggctcaat ccccacaggg
      361 gatccacggt cgccgtgatg aggtcgctca ggtcgtcgag gtacagactg gccaagtgtt
      421 tggcgcggtc cgcggtgccc atggcgccga aggcccatcg gtcgtcctcg cgggcggcga
      481 gccgcgggcc ctcgggcaga aacgcgccgt ccgcgatgcg caccgcgagc gtggggtcgc
      541 ggccttggag gaaggccacc acgggatcga tggtcgccag gtacgcctcc gcgagcgcca
      601 gcgcccgctc ggacgtcgcg gcccactccg gaccccgctc gcgaagacgc gcgaacaggt
      661 cggtcaaccc ggatcgtgag aagaccgcca gcgcggtttc aaggctcagg tcggcctggg
      721 ccgcgtcgat cccgcgcgat gcgagaaaac ccgccaggta ggccgtggcg ggatatgggg
      781 tgttgagttg ggtcagcggc ggcgtgacga gaagaacgcg cacgccggga ttatacgatc
      841 cgacgcctgt tcaggggaag aacgcgggct acggcgcggc gacctcgacc ggggtgtcca
      901 cccaggccga gccgtcccca agctgtccgg agtcgttggc accccaggcc cagagcgtgc
      961 cgtccgcgcg taccgccacg gtgtgatcgc cgaacgtcga cacggtgctc cacgacgccc
     1021 cgaggccaat ctgccctgga acgtcccggg cactggttgt gccgttaccc aattggccga
     1081 gcgtgttccg accccacgtc cacaggctgc cgtcggtctt gattgcaacc gtgtgcccga
     1141 atcccgccgc cgggagcgcc cagttggttg ccgcgtcgag acggatcggc atggaagacg
     1201 atgtggcccc tatcagtccg acctgaccga agaagttgtt cccccacacc cacaactcgc
     1261 cgttgggatt gaccgccacc gtatgttgcg cgccggccgc gatcttgatc cctccgtccg
     1321 gcagcgtcgc cgcgttcatc aaggcactgg taatttcgac gggcgcaaac tggtcggtga
     1381 cgctcccgtc gccgagttgg cccgacgcgt tactccccca ggcccacaac gaaaaactcg
     1441 cgattccgtc caccacgtgc tttttgaccc ccagtgtgtg atacccgccg gccacggccg
     1501 ggtgccaact gtccgaggtt gggatcaggc gaaccgggag gtactcgatc gaggagccgc
     1561 ccgtcgacgc ccccacctgt cccaccgcgt tgtttcccca cgcaaacagc ttgccttgtc
     1621 gcgtgaccgc cacggtgtga tagccgcccg cggagaccgc gacccacggg tcgaacgcca
     1681 ggaattcggg cacctccatc gggaccgacg actgatcgac ctggatgccc accccgagct
     1741 gtcccgatga attatcgccc cacgtgaaca gtcgaccgtc ggaccggatg gccgcggtgt
     1801 gaaacgcacc ggtcgcaacg tgagtccacg tctcggtccc cacgcgcgcg ggcgtttcgc
     1861 tgtcgtaagg cgtcccattg ccgagttgcc cgagcgtgtt gcttccccag gtccacaacg
     1921 atccgtcggc cttgaccgcc gcggtgtgga atccacccgc cgccaccgtg gcgccgctcc
     1981 agtcgacgct cgttccaccg gcgcgcaccg gcacgtattt cgtcgccacg gccccgttgc
     2041 cgatttcacc gtagagattc ccgcccgtca cgaacaatcg gccgttggcc gtgagccgaa
     2101 ccgagtgaaa atagccggcg gccgccgcga accaaccggt cccgatcagc gtcggctcgt
     2161 tcacaaggtc cggcgccgac tcacccaggc ccaattgtcc ataggcgttg ttgccccagc
     2221 cccacaacgt ctgatcgccg gtaatcgcca gggtgtcggt ctcgcccaga accacgctcg
     2281 cccaatccgt gcccgtcccg acctgcatcg gagcgagcgc tccggtcacg gccggaacgc
     2341 ccaattgtcc gtcggcattg tccccccaca cccacagcgt gcccgcggcc gcgccgagag
     2401 ccgtgcgtct gacgcccgcg ctgtgcgagc cgcccgcgtc gatggcgatc cagtcggtct
     2461 cgattcccac ccgggtcggc accaggcggt cgacgggctg gcccccggga accgcggtat
     2521 cgcccacctg gcccgcggta ttgcgccccc atccccagag cgagccgtcc gttgcgatgg
     2581 ccaaggtgtg gtcggtcccg gcggccaccg ccgaccattg catggccagc ggaacccccg
     2641 tcgcgtcggt cacgggccac ggcgtcggga tgatgtatcc gctcgtgttg ttcccggcct
     2701 cgccgtaggt attgtccccc caggtccaca gggtcccgtt gatcttgatc ccggccgtgt
     2761 gacggccgcc cgcagacacg gcgtgccact cgaagtcagc gccgatcttg accggcacat
     2821 tgcgatccgt aaatgtgccg tcgccgagtt ggccggcggc attcgccccc cacgcgtaca
     2881 acgtgccgtt gcgcttgagc gccagggtaa agtcggcgcc ggccgccacg ctgtcccagt
     2941 ccgtggcggt gccgatttgg accgggctcg gccggttgac ggtggtgccg tcgccgagtt
     3001 gcccgtaata attgttgccc catgcccaca acgtgccgtc gctcttgacc gccacggtat
     3061 ggtgcgtgcc cgccgcggcg gagagccaca cacccagcac cgtaaccgtg gccgtggccc
     3121 ggaccgcgcg atccgacgcg ctggtggcga cgatgtggaa gacgcgaccg cccccgccgg
     3181 ccggcgcgcg ataggtcacc tgagacgtgg atgacgtcgg cgaggttgga gtgaacaccg
     3241 ccgcgcccaa cgtgcctccc acgctccctt cttgaatcga ccacaggacg tcgccgtagt
     3301 cattgaccac gtcggtcacc gtcgcggtca ggtcgagcgt gtccccggcc cggaccgacg
     3361 ccgattgggg accgaccgcg accttcaccc gattgaccgc gaccgtggcc gacccgctct
     3421 tcgaatactg atccagcgtg gccgccacgt gatacgttcc tccgagcacg ggcgcggtgt
     3481 acgcccccga ggtcgcgaca atgtcgcccc cgctgagatc ctgcaccgcc caggtccacg
     3541 tgatgaggcc gggggtaggc gccgtatacg tgcgcggttg caaaatgccg tcgttcgtcg
     3601 tgaccgtcgc gccgaaggtg accgtgccct tggcgggcaa tgtcacggtc cttgtggccc
     3661 aggtcgtccc gtcacagggc gcaaccgacc cgtcggttgc cagtggaggg ctgcccgtat
     3721 acacgcagac gccgacagtg atcgcgtcga cgagacggtc ggtcgccacg atgaagtcgc
     3781 cggtcgcccg atccctcact tccgcccgaa gccggtagat gccgggacga tcactggcgg
     3841 tgaactgcgt gtagcttccg gtcggcgtca cgctcggcat cggcagactg ttcgcggtcg
     3901 ccgacggatc aaccggcgtc acgccccaga acacctcgac cagggacgcg ttggtcagac
     3961 gcgcctgaaa cagccgcgtc tccccgttca ccatcggctt ggcgggatcg gcggacaagt
     4021 catcgatctc cacgccggga accggctgat cgaccacggt cacgaacacg gttttcctgg
     4081 tgatcggcgc ggccttggtt tccgccgcca cggtgaaggt cccggtttgg gcgggcgcgg
     4141 tgtagagacc gtcggaagtg atggtccccc cgccggcggg ctcaacgctc caggtgactt
     4201 ccgacgtgga gccccccacc acgcgcgcgg taaac
//
LOCUS       DBNGTT010000147         4222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_540861, whole genome shotgun sequence.
ACCESSION   DBNGTT010000147 DBNGTT010000000
VERSION     DBNGTT010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_540861"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..2754
                     /locus_tag="AC16BC_03360"
     CDS             112..2754
                     /locus_tag="AC16BC_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR3240687.1"
                     /translation="MKYLLNLIASADVPVALHGGRLTLTGASVATATGRVTVTGAVTT
                     TGDADLALDADGQDIAAIAGALALPFPTAGVGTFRGRLTGPLASPLVDGRASIRDLAL
                     RGHPAGNLAATLRYAERRLTIRDTDWRKGDARYDTVGTLDWTGPSRPAFDLIATLRRA
                     RVDEVLPIAFRELPIITAADGTFTFKGAPADFVLAGDLTVGSGSIWGQTFDDGVVRMT
                     IDHQRVTFLRAALRRGESEVRGRGSIRYHEGFEARFEAPKVYLQSLNIFALNRLPISG
                     TASASLDMHGAFDRPEMHGTATIAYLEGAGQALGAGTAKLDVADHRLALAVSLDDQHA
                     KLSATLRWEPSFPWSGDLALENSSLIPLLRPWLPPALAGLNASLTGRVAIEGPLRDPL
                     HWKVRARISQLSADVGEYVVENQDEIALALDGGRFTVESCRLRGTDTTLTISGGADLF
                     KEYHLLIVGEADLRLTRLFVPTITSGRGKTYLVLKISDRWDAPKIQGGVTIQSAWIQT
                     RTLRQRITIETVGLFFNERQILLESLEGSVGNGRVSASGRIELAGFRPNSIGVLIDIA
                     GVQFPLTETLIPTFSGQLALTITPKEQHLRGELTVDRAIYNTRIDVQQWLVDFRKRAD
                     IRQDLDVPALTRGVALNIHFSGRDQIGIKNNLADVDLDVDVFLRGSVDRPFLTGKIET
                     RDGRMTFRNNVFEIQGATVDFTDPARFNPRLDLHATTKVQSYNIAMHLNGTINHFDLD
                     LTSDPSLSETDILALLAVGRTAEQVSRSQTTVGRDEAVSIAMQKLLEEGIQRLPGVDR
                     VVDVFQIDQEYDPTLDVSSPRLSVGKQLFNDRLILRYSTTLDQSARQGIRLEYELSRK
                     VFLSGEQDSTRGFGGDIRFRFDFR"
     gene            2751..>4222
                     /locus_tag="AC16BC_03365"
     CDS             2751..>4222
                     /locus_tag="AC16BC_03365"
                     /inference="COORDINATES: protein motif:HMM:NF018899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR3240688.1"
                     /translation="MRARAPLIAAAALSALLVAPDGAAADDPSPSLVSFEGRPIVSIE
                     VRGPTAPTADIVEQKTPLRTGRSYSAVAIRGAMDTLMKLGDWTDIRVEADPHDAGVAL
                     TLAFIPRLRISAVEFRGHHQSTAALRKAAGLRPGDPVTDAALSAAVARVRAWYEQFGY
                     VVDITARTASDAQNTRARVTFAIRERDRLRLRSITFPGTPAFSRLRHLVALRTAPGEY
                     VSRSHLERGIDRLRRLYHGKGYLTAVIGPMAWTATPAGLAIVVPIQQGPQYRVAVSGF
                     DDEITAQMTFFDDPRDDEDSLAEESARLTRYLSDQGYRRAAVRMHREVAPDGTVVIRV
                     AVDAGPRFPLREILITGNVAASDRDLQQVLETRPGGWWRARYIQRDVLEADGERIQAW
                     YRAHGFLSTSVTVGATEADAPPRAAAVFTVDEGPQTRIGLLTIEGREAVSLDDVLSNY
                     DLREGSPYVEARFRAARAAMLERYSERGYIYAKIDGTPAI"
BASE COUNT          744 a         1441 c         1342 g          695 t
ORIGIN      
        1 gctacgaagg caagtcgcgc gagcttatgt ttccggccac actggattcc gcacttgcct
       61 ggcaaccgcc gagagtcgag acggaggaat ccagtgagga aatgaaccgc gttgaaatat
      121 ttgctgaacc tgatcgcgtc ggcggatgtg cccgtggcgc ttcacggcgg acgactgacg
      181 ctgaccggcg cgtcggtcgc caccgccacc ggtcgcgtca cggtgaccgg cgcggtgacg
      241 acaaccggcg acgcggatct ggcgctcgac gccgacggcc aggacatcgc cgcgatcgcc
      301 ggcgcgctcg cgctgccctt ccccacggcc ggggtgggga cgttccgcgg tcgtttgacc
      361 ggcccgctcg cctcgccgct cgtggacggc cgcgcgagca tccgcgacct ggccttgcgg
      421 ggccatcccg ccggcaatct cgccgccacc ctgcgctacg cggaacgccg gttgaccatt
      481 cgcgacacgg actggcgcaa aggcgacgcg cgatacgaca cggtgggaac cctcgactgg
      541 accggtccgt cgcgtccggc cttcgacctg atcgccacgt tgcggcgggc ccgcgtggac
      601 gaggtattgc ccattgcatt tcgggagttg ccgatcatca ccgccgcgga cggcacgttc
      661 accttcaagg gcgcgcccgc cgactttgtc ctggccggcg acttgaccgt gggctcggga
      721 tcgatctggg gtcagacgtt cgacgacggc gtggtccgca tgaccatcga ccaccaacgc
      781 gtgaccttcc tccgcgcggc gctgcgacga ggcgaaagcg aggtgcgggg acggggatcg
      841 atccggtacc acgagggatt cgaggcccga ttcgaggcgc ccaaggttta tctccagagc
      901 ctgaacatct tcgcgctgaa ccgcctcccg atctccggca ccgcgtcggc ctccctggac
      961 atgcacggcg cgttcgaccg acccgagatg cacggcaccg cgaccatcgc ctacctggaa
     1021 ggcgcggggc aagccctcgg cgcgggcacc gcgaagctcg acgtcgcgga ccaccgactc
     1081 gccctcgccg tctcgttgga cgaccagcac gccaaactct ccgcaacact gcgctgggag
     1141 ccgtcgttcc cgtggtcggg cgacctggcg ctcgagaaca gctcgctcat tccgctgctt
     1201 cgaccgtggc tgccccccgc gctggcgggc ctgaacgcat ccttgaccgg acgcgtcgcg
     1261 atcgagggcc cgctgcgcga tcccttgcac tggaaagtca gggcccgcat cagtcaactc
     1321 agcgcggacg tgggcgaata cgtggtggag aaccaggacg agatcgccct ggcgctcgac
     1381 ggcggccgct tcaccgtgga atcgtgtcgc ctgcggggca cggacaccac cctgaccatc
     1441 tcgggcggcg ccgatctctt caaggagtat cacctgctga tcgtggggga agccgacctg
     1501 cgactgaccc gcctgttcgt ccccacgatc acctcgggac gcggcaagac ctacctggtg
     1561 ctgaaaatct cggaccggtg ggacgccccc aagatccagg gcggcgtcac gatccagagc
     1621 gcgtggatcc agacccgcac actccgtcaa cgtattacga tcgaaaccgt gggcttgttt
     1681 ttcaacgagc gccaaattct gctggagtct ctcgaaggct cggtcggcaa cggccgcgtc
     1741 tcggcgagcg ggcggatcga actcgcgggg ttccggccca acagcatcgg cgtactcatc
     1801 gatatcgcgg gcgtgcagtt cccgctcacg gagacgctca tccccacgtt cagcgggcaa
     1861 ctcgcgctca ccatcacgcc gaaggagcaa cacctccgtg gcgaactcac ggtggatcgc
     1921 gccatctaca acacccggat tgacgtccag cagtggcttg tcgactttcg gaaacgggcc
     1981 gatataaggc aagatctaga cgtacccgct ctcacgcgcg gcgttgccct aaacatccat
     2041 ttctccggtc gagaccagat cggcatcaaa aacaacctcg cggacgtcga cttggacgtc
     2101 gatgtatttc tgcgaggaag cgttgaccgt ccgtttctaa ctgggaagat cgaaacgcgg
     2161 gacggccgta tgacgttccg caacaacgtg ttcgaaatcc aaggagcgac ggtcgatttc
     2221 accgatcccg cacgattcaa tccgcgactc gacctgcacg ccaccaccaa ggtccagtcg
     2281 tacaacatcg ccatgcatct aaacggcacc attaaccact tcgatttgga tttgacctcg
     2341 gacccgtctt tgagcgaaac ggacattctc gcgcttctgg ctgtgggtcg aaccgccgaa
     2401 caagtctctc ggtcccaaac caccgtgggg cgtgacgaag ccgtgtccat tgccatgcag
     2461 aaactgctgg aggaaggtat tcaacggctc ccgggagtcg accgggtggt cgacgtcttc
     2521 cagatcgatc aggagtatga tccgacgctt gacgtgagtt cgccgcggct ctcagtcggc
     2581 aaacaactct tcaacgaccg tttgatactt cgatacagta caacgctgga ccaatcggct
     2641 agacaaggga ttcggcttga atacgaactt agccggaagg tctttctgag tggcgagcag
     2701 gactccacgc ggggattcgg gggagacatc cgattccgat tcgactttcg ttgagagccc
     2761 gcgcccccct gatcgcggcc gccgcgttga gcgcgttgct cgtcgcgccc gatggagcgg
     2821 ccgccgacga tccgagtccg tccctcgtgt cgttcgaggg gcgcccgatc gtgtcgatcg
     2881 aggtgcgcgg tcccaccgcg ccgaccgcgg acatcgtcga gcaaaagacc ccgctccgaa
     2941 ccggacgctc gtactcggcc gtggcaatcc gcggcgccat ggacacgctg atgaaactcg
     3001 gggactggac cgacattcgc gtggaggccg atccgcacga tgccggggtc gcgctgaccc
     3061 tcgccttcat tccccggctt cggatcagcg cggtggagtt tcggggccac caccaatcga
     3121 ccgccgcgct gcgaaaagcc gcgggactcc gccccgggga tccggtgacc gacgccgcgc
     3181 tctcggccgc cgtcgctcgc gtgcgagcct ggtacgagca gttcggatac gtggtggaca
     3241 tcaccgctcg gaccgcgtcg gatgcccaaa acacccgcgc gcgggtgacg ttcgcgatcc
     3301 gggagcggga ccgcctccgg ctccgcagca tcacattccc aggcacgccg gccttctcgc
     3361 ggctccgaca cctggtcgcg ctccggaccg cccccggaga gtacgtctcg cgcagccact
     3421 tggagcgggg catcgaccgc ctccgccggc tctatcacgg caagggatac ctcaccgccg
     3481 tgatcggccc gatggcgtgg accgccacgc cggccggcct ggcgatcgtg gttccgatcc
     3541 aacagggtcc ccaataccgg gttgcggtgt ccgggttcga cgacgagatc accgcccaga
     3601 tgacgttctt cgatgatccg cgggacgacg aggactcgct cgccgaagaa agcgcgcggc
     3661 tcacgcggta tctctccgat caagggtatc gccgcgccgc ggtgcgcatg caccgcgagg
     3721 tcgctccgga cggcaccgtg gtcatccggg ttgcggtgga cgccgggccg cggttccccc
     3781 ttcgggagat cctgatcacc ggcaatgtcg ccgcgtcgga ccgcgacctg cagcaggtgc
     3841 tggagacgag gcccggtggg tggtggcgcg cccgctatat ccagcgcgac gtgctggagg
     3901 ccgacggaga acggattcag gcgtggtatc gcgcgcatgg attcctctcc acctcggtga
     3961 ccgtcggggc gacggaagcc gacgcccccc cacgggccgc ggcggtgttt acggtcgacg
     4021 agggtccgca aacccgcatc ggactcctca cgatcgaggg gcgcgaagcc gtctcactcg
     4081 acgacgtgtt gtcgaactac gacctgcgcg agggatcgcc atacgtcgaa gcgcggtttc
     4141 gcgcggcgcg cgcggcgatg ctcgaacgct acagcgaacg gggatacatc tacgcgaaaa
     4201 tcgacggcac gcccgcgatc ag
//
LOCUS       DBNGTT010000148         3865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_669263, whole genome shotgun sequence.
ACCESSION   DBNGTT010000148 DBNGTT010000000
VERSION     DBNGTT010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_669263"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="AC16BC_03370"
     CDS             <1..804
                     /locus_tag="AC16BC_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3240689.1"
                     /translation="VTSLPVGPDGTLDLDVLKDMVTEHTALVSMMWANNETGVVFPVA
                     EAVGVAKNKGAMFHTDAVQAAGKIPVNLAETPADFLSFSGHKLHAPKGIGVLFVRKGL
                     TLPPLLFGHQERGRRGGTENVPGIVALGKACELATGRSGDMERVRTLRDRLEKGLLRA
                     VPTAVVNGRRDVRVPNTANIRFGTIDAEAVLIRLNRDGIAAAPGSACSSGGTEPSHVL
                     TAMGLSREQALASVRFSLSGYTTLVEISRVLEVLPTIVEELTALELVRG"
     gene            806..1009
                     /gene="nifT"
                     /locus_tag="AC16BC_03375"
     CDS             806..1009
                     /gene="nifT"
                     /locus_tag="AC16BC_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HIR3240690.1"
                     /translation="MKVMIRKNSEGVLSAYVSKKDLEEPIVATEKETLWGGVVTLANG
                     WKLALPEMAHDTRLPITVEARRL"
     gene            1054..2145
                     /locus_tag="AC16BC_03380"
     CDS             1054..2145
                     /locus_tag="AC16BC_03380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3240691.1"
                     /translation="MAEVLRSSRFGSGAMGEAFEEVFSEYAGREYAAAVSSGTLALLL
                     CLRGYGIGPGDEVVVSPFSWYQIAHAVSLAGAEAVFADIDYWTCTLDPAKAEQKITEK
                     TKAILVGNTNGHPADWDAFRALARRYHLRLIEDSTEAIGSLYKGMAVGRFGDCAIFSL
                     CAPGPLTTGGCAVVVTDDGDLARAVRHGRSRRLEDRFSVVVTGTLPLEARVNDLSAAL
                     GLAQLSRPEEILAQRKAVEAWYAREMQSFEGIKDPFVAPYATEVHWFLYMVHLGTRFG
                     RSSRDAIIEDLLTEEVEGAAYCQPAHLQRFYIERGSRKGACAIAEKNGDRAIALPFHG
                     HLTEDQVVFIVKTLKDASINVGAGSAIYL"
     gene            2158..2385
                     /locus_tag="AC16BC_03385"
     CDS             2158..2385
                     /locus_tag="AC16BC_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240692.1"
                     /translation="MDSNQIMIHVRFAPDGTVTEIGERPTGITAQEWFNHLSRNTENG
                     YQALSGGRGIFRLPREQVEAFKRLSAVKEPA"
     gene            2382..3266
                     /locus_tag="AC16BC_03390"
     CDS             2382..3266
                     /locus_tag="AC16BC_03390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3240693.1"
                     /translation="MTTGRDRDIFEEIAVGLVEGTIIPYLGPGVFQTQAGFPVGPTEL
                     ADWLCHKVAVPGRIRGNLTAVAQYIENFKHRKTLTHLMNEAFTPETVPAPAHRLLSAL
                     PRLPLVVDTWYDGTTTRVLRGSSEHGRTVCQITGVSRSEHPNDWVRYTGGGDSPEAAE
                     MAETVLYKPHGSVHPEPNFLISDTDYVEVLTEIDIQTPIPPVVQRLRTERHFLFLGCR
                     FNQQLDRIFARQIMKRSSDRHWAILDEEVTKKEARFLAVQNIAPIRVGLGEFIEELTS
                     RLAVASKAAEEPVPCKSA"
     gene            3251..3700
                     /locus_tag="AC16BC_03395"
     CDS             3251..3700
                     /locus_tag="AC16BC_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3240694.1"
                     /translation="MQIGVEASRAVENKRIFVVEPDEVTRAALQFMLHDENETHEVRD
                     LETAYRKGRDSQPDLLLLGMGIVRKKGTTVLKEIKSVFPGAKLIPVAEADEALAVEEA
                     LRAGADDVLLKPLRVEAVRETVDRHLGRNGISHGGESNPHSERNSRL"
BASE COUNT          752 a         1085 c         1303 g          725 t
ORIGIN      
        1 gtgacgtccc ttcccgtggg gccggatggc accctggatc tcgacgtcct caaagacatg
       61 gtcacggaac acacggccct ggtctccatg atgtgggcga ataacgaaac cggagtagtg
      121 ttcccggtcg ctgaggcggt cggggtggca aagaacaaag gagccatgtt tcacaccgac
      181 gcggtccagg ccgctgggaa gattccggtc aacctggcag agactcccgc tgacttcctc
      241 tcattttccg gccataaact gcacgccccc aaggggatcg gcgtcctctt tgtccggaag
      301 ggactgacgc tcccgcccct gttgttcggt caccaggagc ggggaaggcg agggggaacc
      361 gaaaacgttc cggggatcgt ggcgctggga aaggcctgtg agctggcaac cggcaggagc
      421 ggcgatatgg aacgggtgcg tacgctgaga gatcggttgg agaagggctt gctccgtgct
      481 gttccaaccg ccgtggtcaa cggccgccgc gacgtgcggg tccccaacac cgccaatatt
      541 cgattcggaa cgatcgatgc agaagccgtc ttgatccggc tgaaccggga cgggatcgcc
      601 gctgcccccg gttcggcctg ttcctccggc ggtacggagc cgtcgcacgt gttgaccgcg
      661 atggggctgt cgcgcgaaca ggcgctcgcc tcggtccgat tttcgttgag cggctatacg
      721 acgctcgtgg agatctccag ggtcctggag gtgctgccga cgattgtgga ggaattgacc
      781 gcgctggagt tggtacgggg ctgagatgaa ggtgatgatc cgaaaaaatt ccgagggcgt
      841 cctctcggcc tacgtgtcga agaaggatct ggaggagccg atcgtggcga cggagaagga
      901 gactctctgg ggaggggtgg tgacgctcgc caacggatgg aaactggcgt tgccggagat
      961 ggcccatgac acccggctgc cgatcaccgt ggaagcgcgc cgactatgat cgccctatcg
     1021 gaaccagacc tctcgccttt ggagtcagag gtggtggccg aggtcctccg ctcctcccga
     1081 ttcggttccg gggcgatggg ggaggccttc gaggaggtgt tttcagagta tgccggccgg
     1141 gagtatgcgg cggcggtcag cagtgggacc ctggccttgc tcctttgttt gagggggtat
     1201 ggaatcgggc cgggcgatga ggtcgtggtc tcgccgttct cctggtacca gatcgcccac
     1261 gccgtctcgt tggcgggtgc cgaagcggtg tttgccgaca tcgactattg gacctgcacc
     1321 ctcgaccctg ccaaggctga acagaagatc acggagaaga cgaaggccat tctggtgggc
     1381 aataccaacg gccaccccgc agactgggac gcctttcggg cgctggccag gcggtatcac
     1441 ctgcggttga tcgaggactc cacggaagcg atcggctcgc tgtacaaagg gatggcggtg
     1501 ggtcggttcg gggactgcgc gatcttctcc ctctgcgcgc ccgggccctt gacgactggt
     1561 ggctgcgcag tggtggtgac ggacgatggc gatctggccc gggccgtcag gcatggacga
     1621 agtcggcgcc tggaggaccg gttctccgtg gtcgtgaccg gcacgctccc cctagaggcc
     1681 agggtcaacg atctctcggc ggctctgggg cttgcgcaac tgagccggcc cgaagagatc
     1741 cttgcgcagc ggaaagcggt ggaagcctgg tatgcccggg agatgcaatc ctttgagggg
     1801 atcaaggacc ccttcgtcgc tccgtacgct accgaagtcc actggttcct ctacatggtt
     1861 cacctgggga cccggttcgg ccggagcagc cgggacgcga tcatcgaaga cctgctgacc
     1921 gaagaggtcg agggggcggc gtattgccaa ccggcgcatc tacaacggtt ttatatagag
     1981 cgcggaagtc gtaagggagc gtgcgccatt gcggagaaaa atggggatcg ggcgatcgct
     2041 ctccccttcc atggacatct gacggaggat caggtggtgt ttatcgtgaa aaccttgaag
     2101 gatgcctcga tcaacgtggg ggccgggtcg gccatttatt tgtaagacga gggagcgatg
     2161 gactcaaacc agatcatgat tcacgtccgg ttcgccccgg acggcaccgt gacggagatc
     2221 ggcgagaggc ctaccgggat cacggcgcag gagtggttca accacttgag ccggaacacg
     2281 gagaatggct atcaagccct ttccggagga cgggggatct tccgtctgcc ccgagaacag
     2341 gtggaggcgt tcaaacgcct ctcggcggtg aaggagccgg catgacgacg ggaagagacc
     2401 gggacatatt tgaagagatt gccgtcggtc ttgtggaggg aacgatcatt ccctacctcg
     2461 gtccgggagt cttccagaca caggccggct ttcccgtcgg tcccacggag ctggcggact
     2521 ggctctgtca caaggtcgcg gttcccggcc ggatccgggg gaatctgacg gccgtggccc
     2581 agtacatcga gaacttcaag caccggaaga ccctcaccca tctgatgaac gaggccttca
     2641 ctccggagac cgtccccgct ccggctcacc ggcttctgtc ggcgctgccg cgcctgccgt
     2701 tggtggtgga cacctggtat gacggcacca cgacccgggt gcttaggggt tccagtgaac
     2761 acggtcgaac cgtgtgtcag atcactgggg tgagccgctc ggagcatccg aatgattggg
     2821 tccgatacac cggaggtggt gactccccgg aggcggccga gatggcggaa acggttctct
     2881 acaagcccca tgggtccgtt catccagagc ccaactttct gatttccgat acggactacg
     2941 tcgaggtgtt gaccgagatc gatatccaga ccccgatccc tccggtcgtc cagcggcttc
     3001 ggacggaacg acacttcctt tttctgggat gccgattcaa ccagcaattg gatcggatct
     3061 tcgcccgaca gatcatgaag cgttcctccg atcggcattg ggccatcctt gacgaggagg
     3121 taaccaagaa ggaggcccga tttctggctg ttcagaatat cgcgccgatc agggtcggcc
     3181 tgggggagtt cattgaagaa ctgaccagcc ggttggccgt tgcgtccaag gctgcggagg
     3241 agccggttcc atgcaaatcg gcgtagaagc ctcacgcgcg gtcgagaaca aacggatctt
     3301 cgtggtggag ccggatgagg tgacccgtgc ggccctccaa tttatgctcc acgatgagaa
     3361 cgagacccac gaggtacgcg acttggagac ggcctatcga aagggccggg acagtcagcc
     3421 ggacctgctg cttctgggaa tgggcatcgt gcggaagaaa gggacgacgg ttctgaaaga
     3481 gatcaaaagc gtgtttcctg gcgcgaaact catcccggtg gccgaggccg atgaggcgct
     3541 tgccgtcgaa gaggcgcttc gggccggggc ggatgatgtg ttgctcaaac ccctgcgggt
     3601 ggaagcggtg agggaaacag tggatcgcca tcttggacgg aacgggatct ctcacggcgg
     3661 ggaatcgaat ccgcacagcg agcgtaattc ccgcctgtaa aggcggggtc agcgcgcgta
     3721 tacaaatacg gctttctcca tccggattct tcaataaagg aacgagtcag atgaacgcga
     3781 cggaaatggt caccggcgtc acccagggcg ggaaggtctg gattccccaa ttcgtccaga
     3841 gcatcgattc gtcaaagtgt attgg
//
LOCUS       DBNGTT010000149         4893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3480032, whole genome shotgun sequence.
ACCESSION   DBNGTT010000149 DBNGTT010000000
VERSION     DBNGTT010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3480032"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="AC16BC_03400"
     CDS             complement(<1..336)
                     /locus_tag="AC16BC_03400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3240695.1"
                     /translation="MTPQDLAKQLAAGRIDPLYLVVGEESWLADEALSALRRYAAPPD
                     DLMNSHVFSGSEIAPAELVAIAETLPAMAPRRFIMVRDAERLASSDVLSGYCSDPSPT
                     TCMVFLSAKL"
     gene            complement(333..887)
                     /gene="lptE"
                     /locus_tag="AC16BC_03405"
     CDS             complement(333..887)
                     /gene="lptE"
                     /locus_tag="AC16BC_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HIR3240696.1"
                     /translation="MSWDSLTAAALATALVAGCGYGILSSRDGTGATSVAVPPFENRT
                     FEPYVDARVTERVRSRLLTAGPWRLVSTPESADVVIRGIVTNFSVITLSFDAANRPVE
                     QRVAIAADVTAAPKQGVPFHNTITATAEYRESADSLRTRTAKNRAIEEAAENVAQELT
                     ARLSMHLQAQGTSAPRPAPLPAAP"
     gene            complement(874..3354)
                     /gene="leuS"
                     /locus_tag="AC16BC_03410"
     CDS             complement(874..3354)
                     /gene="leuS"
                     /locus_tag="AC16BC_03410"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3240697.1"
                     /translation="MNPKYDPKAIETRWQTAWDEARLFTTPAPDARPKFYCLAMFPYP
                     SGRIHMGHVRVYTIGDVIARYQRMRGRAVLHPIGWDAFGMPAENAAIKQGVHPATWTY
                     DNIAHMRTQLKRLGLSYDWSREVTTCDADYYRWNQWFFVKMFERGLAYKKFSAVNWCP
                     SCETVLANEQVVDGGCWRCDTPVTSRELSQWFFKITAYAEELLAGCDRLTGWPERVLT
                     MQRNWIGRSEGVEVEFPIEGASQSVRIFTTRPDTIFGVTFMTLAPEHPLVESLIAGKP
                     EAAAVRAFVERVRAQDPRARTADTATKEGVFTGAYAVNPLSGDRVPIWIGNFVLMGYG
                     TGAVMAVPAHDQRDFEFATRHGLPVRVVIQNPAGTLSASDLPAAYVEEAGRLVESGEF
                     CGLPPIDAQRRIAEAVEQRRLGRRVVNYRLRDWGVSRQRYWGTPIPIVYCDRCGMVPV
                     PEDQLPVTLPHDVPFTGRGGSPLAESRAFVETACPTCRGPARRETDTMDTFVDSSWYF
                     LRYTDPPPRGPRALDVDAAARWMPVDQYVGGIEHAVLHLLYARFFTKVIRDLGMIHVD
                     EPFTGLLTQGMVIKGGAKMSKSKGNVVDPDQLVSTYGADTARLFILFAAPPEKDLEWS
                     DEGVEGVHRFLGRVWRLVASWLETGAPQTTGTHDTDEQVSRIRRAMHRTIRRVTGDIE
                     DGYHFNTAVAGLMEYVNALSEFKAPESPALRPAMIQALDTLAVLLAPFAPHLAEELWA
                     ALGHQPSVAQQSWPVADPAWLVDARVTVPVQINGKLRGTIVVATAASQDDVVAAAFAD
                     PKIRGWLGDRAPKKVVYVPGRLVNLVLG"
     gene            3491..3826
                     /locus_tag="AC16BC_03415"
     CDS             3491..3826
                     /locus_tag="AC16BC_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HIR3240698.1"
                     /translation="MATSYTPVEIAKEGEGVIRITWDDGHQGRYGTRYLRAACQCAVC
                     VDEWTGERKVTIESIPEDIRALKIAPVGQYGIHIDWSDGHGTGIYAFDVLRKLCPCSD
                     CMVRPISAL"
     gene            3900..4148
                     /locus_tag="AC16BC_03420"
     CDS             3900..4148
                     /locus_tag="AC16BC_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240699.1"
                     /translation="MDEWDEGKQRLTELVKNTASDVTVVIPTRPTGGMFLIALARGKA
                     KKFVSVSEDDLIDLVEDHAVETDVMATVNGALRELAGG"
     gene            4151..4762
                     /locus_tag="AC16BC_03425"
     CDS             4151..4762
                     /locus_tag="AC16BC_03425"
                     /inference="COORDINATES: protein motif:HMM:NF025948.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3240700.1"
                     /translation="MREVVPGVYEWSWFSDDKQINFNGHLVIEGRDRVLIDPPPMTEE
                     DRAEIARQGGVTAIVLTNRDHVRESAACQRSYGAPVWVPAADTAWVDVRFDRTYEHAS
                     HVPGGLVAIRVPDAKSPGESALWSASKHALIVGDAVIGKPPGALGMLPDEKFADPARA
                     LAGLRVLLDARYDYDAVLVGDGVSIPTGGHAELERLAVAGRAA"
BASE COUNT          848 a         1565 c         1702 g          778 t
ORIGIN      
        1 aagcttggcg gacaggaaga ccatgcaggt ggtcggcgag ggatcgctgc agtagccgga
       61 taagacatcg gaggacgcga ggcgttccgc gtcacgcacc atgatgaagc gacgcggagc
      121 catggccggg agcgtctcgg cgatggccac caactccgcg ggcgcgatct ccgaacccga
      181 gaacacgtgc gagttcatca ggtcgtccgg cggcgccgca taccgccgca aggcggagag
      241 cgcctcatcg gccagccacg actcctcgcc cacaaccagg tacaacgggt cgatccggcc
      301 ggcggcaagc tgcttggcga ggtcctgggg ggtcacggcg cggcgggaag gggcgcgggc
      361 cttggcgcgg aggttccttg tgcttgcaga tgcatggaca gtcgagcggt gagttcctgc
      421 gccacgttct ccgccgcctc ctcgatcgcg cggttcttgg cggtccgcgt ccgcaagctg
      481 tccgcggact cccggtattc agccgtcgcg gtgatggtat tgtgaaacgg cacgccctgt
      541 ttgggcgcgg ccgtgacgtc cgcggcgatc gcgacgcgct gttccaccgg acgattggcg
      601 gcatcgaacg acagggtgat gacgctgaag ttggtcacga tgccgcggat caccacgtcg
      661 gcggattccg gcgttgagac gagcctccac ggaccggcgg tcagcaggcg cgagcggact
      721 cgttcggtga cgcgcgcgtc cacgtacggt tcgaacgtgc ggttctcaaa cggcgggacc
      781 gccaccgatg tcgctccggt cccatcgcga gacgacagga tgccgtagcc gcaccccgca
      841 acgagcgccg tcgcgagcgc ggcggccgtc aggctatccc aggacaagat tcaccaggcg
      901 gccggggacg tagaccacct tcttgggcgc gcggtcgccg agccatccgc ggatcttggg
      961 gtccgcgaac gccgccgcca ccacgtcgtc ctgcgaggcc gccgtggcga ccacgatggt
     1021 gccgcgcagc ttgccgttga tctgcaccgg gaccgtcacc cgggcgtcga ccagccacgc
     1081 gggatcagcg accggccacg attgctgcgc gaccgacggc tgatgcccca acgcagccca
     1141 cagttcttcg gccaagtgcg gcgcaaacgg ggcgagcaac acggcgagcg tgtcgagcgc
     1201 ctggatcatc gcgggacgga gcgcgggaga ttccggggcc ttgaactccg acaaggcatt
     1261 gacgtattcc atgagcccgg cgaccgcggt gttgaagtgg tacccgtcct cgatgtcgcc
     1321 ggtgacccgg cgaatcgtgc ggtgcatggc gcggcggatg cgcgacacct gctcgtcggt
     1381 atcgtgggtg cctgtggtct ggggcgcgcc ggtctcgagc cacgaggcca ccaaacgcca
     1441 cacgcgcccc aggaatcgat gcacgccctc cacgccttcg tcgctccact ccaagtcctt
     1501 ttccggaggc gccgcgaaga gaatgaagag cctcgcggtg tccgcgccgt aggtggacac
     1561 gagttggtcg gggtccacca cgttgccctt ggacttggac atcttggccc cgcccttgat
     1621 gaccatgccc tgcgtgagca ggccggtaaa cggctcgtcc acgtgaatca tccccaggtc
     1681 gcgaatcacc ttggtgaaaa aacgggcgta gagcaggtgg agcacggcgt gttcaatccc
     1741 gcctacgtac tgatccaccg gcatccaccg ggccgctgcg tcgacgtcca gcgcccgcgg
     1801 gccgcgcggc ggcggatcgg tataccgcag gaaataccag gacgagtcca cgaacgtatc
     1861 catggtgtcg gtttcccggc gcgccgggcc gcgacacgtc gggcacgcgg tctccacgaa
     1921 cgcgcgactc tcggcgagcg gggagccgcc gcgtccggta aacggcacgt cgtgcggcag
     1981 cgtgaccggc agttggtcct cgggaaccgg caccatcccg cagcggtcgc aatacacgat
     2041 cgggatcggg gtgccccagt agcgttgccg cgagacgccc cagtcacgaa ggcgatagtt
     2101 gaccacgcgg cggccgaggc ggcgctgctc cacggcctcc gcgatgcggc gttgcgcgtc
     2161 gatcggcggc agcccgcaga actccccgga ctccaccagc cgaccggcct cttcgacgta
     2221 cgccgccgga agatcggacg cgctcagggt gcccgcgggg ttttggatca cgacgcggac
     2281 cggcaggccg tgccgggtgg cgaattcaaa gtcgcgctga tcgtgcgcgg gcacggccat
     2341 caccgccccg gtgccgtacc ccatcagcac gaagttgccg atccagatgg ggacgcgatc
     2401 gccgctcaag gggttgaccg cgtacgcgcc ggtaaagacg ccttctttgg tcgcggtgtc
     2461 cgcggtgcgc gcccggggat cttgagcccg cacgcgttcc acgaacgcgc ggaccgccgc
     2521 ggcctccggt ttgcccgcga tcaacgactc caccagaggg tgttcgggcg ccagggtcat
     2581 gaaggtgacc ccgaagatcg tgtcgggccg ggtggtgaag atgcggaccg actggctggc
     2641 gccctcgatc gggaactcca cctccacgcc ctcgctgcgt ccgatccaat tgcgctgcat
     2701 ggtgagcacg cgctcgggcc agcccgtgag ccggtcgcac cccgcgagca gttcctccgc
     2761 gtaggccgtg atcttgaaga accactgcga gagttcgcgc gaggtgaccg gcgtgtcgca
     2821 gcgccagcaa ccgccgtcca cgacctgctc gttcgcgagc acggtttcgc aggaggggca
     2881 ccaattcacg gccgaaaact tcttgtacgc gagcccgcgc tcgaacatct tgacgaagaa
     2941 ccactggttc cagcggtagt agtccgcgtc gcaggtcgtg acttcgcgcg accagtcgta
     3001 ggacaggccc aggcgtttga gctgggtgcg catgtgcgcg atgttgtcgt aggtccacgt
     3061 ggccgggtgc acgccctgct tgatcgccgc gttttccgcg ggcatgccga acgcgtccca
     3121 cccgatcgga tgcagcacgg cccgtccgcg catccgctgg tagcgcgcga tcacgtcgcc
     3181 gatggtgtac acccgcacgt gccccatatg aatgcggccc gacggatacg ggaacatggc
     3241 gagacagtag aacttcggtc gcgcgtccgg cgcgggggtg gtaaacaggc gagcctcgtc
     3301 ccacgcggtt tgccaacgcg tttcaatggc cttcggatcg tacttggggt tcatcgcggc
     3361 ggcgctcgaa tggcctgtta cataacacag tcgcgcgcaa cgcggcaagc aggccgcgac
     3421 gcaggtcccg gagttgcgcg aaaacgaagg cttggagtag agtacccatc tcgaaccgac
     3481 acggatggcc atggcgacat cgtatacgcc ggttgaaatc gcgaaagaag gagagggcgt
     3541 gatccgcatc acctgggacg acggtcacca gggccgatac ggcacgcggt acctcagggc
     3601 ggcgtgccag tgcgcggtgt gcgtggacga gtggaccggc gaacgcaagg tcacgatcga
     3661 gtcgatcccg gaggacatcc gcgcgctcaa gatcgcgccc gtgggccagt acgggattca
     3721 tatcgactgg agcgacgggc acggcacggg catttacgcg ttcgacgtgt tgcgaaagct
     3781 ctgtccgtgt tcggactgca tggtccggcc gatctcggcg ctgtaggtcg gcgtccgtcc
     3841 ggtgaaccgg cgtggcggat cgagacgaga ctcaggcaac catgagacgg taggcgacaa
     3901 tggatgagtg ggacgagggg aaacaacgat tgaccgaact ggtgaagaat accgcgtccg
     3961 acgtgaccgt ggtgattccc actagaccga cgggcgggat gtttctgatc gcgctcgcgc
     4021 ggggcaaggc caagaagttc gtctcggtgt ccgaggacga cttgatcgat ctggtggaag
     4081 accatgccgt ggaaaccgac gtgatggcga ccgtcaacgg cgcgctgcgc gagctggcgg
     4141 gagggtgacc gtgcgcgagg tggtgcccgg cgtctacgaa tggtcgtggt tttccgatga
     4201 caagcagatc aatttcaacg gccatcttgt cattgaggga cgcgaccgtg tgctgatcga
     4261 tccaccgcct atgacggagg aggatcgcgc cgaaatcgct cggcaaggcg gggtgacggc
     4321 gatcgtgctc accaatcgcg accacgtgcg tgaaagcgcg gcgtgccagc ggtcgtacgg
     4381 cgcgcccgtc tgggtgcccg cggcggacac cgcgtgggtg gacgtgcggt tcgatcgcac
     4441 ctacgaacac gccagccacg tgccgggcgg tctcgtggcg atccgtgtgc ccgacgccaa
     4501 gtccccgggt gaatccgcct tgtggtcggc cagcaaacat gcgttgatcg tgggcgacgc
     4561 ggtcatcggt aaaccgccgg gtgccttggg catgttgccc gacgagaagt tcgcggatcc
     4621 ggccagggcg ctcgccggcc tacgcgtact gctcgacgcc cggtacgact acgacgcggt
     4681 gttggtgggg gacggggtgt cgattccgac cggcggccat gcggaactcg aacgcctggc
     4741 ggtcgcgggt cgcgccgcat aacgggaggt ctcgatgttc agtattttca accgatacga
     4801 tcaaacgccc ggcggggtca atatggccgc gagcgaggtc aaacaacgcc tggaccgcgg
     4861 ggacaaggtc gtgctcttgg acgtgcgcga aca
//
LOCUS       DBNGTT010000150         2654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2969188, whole genome shotgun sequence.
ACCESSION   DBNGTT010000150 DBNGTT010000000
VERSION     DBNGTT010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2969188"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /gene="tsaA"
                     /locus_tag="AC16BC_03430"
     CDS             <1..302
                     /gene="tsaA"
                     /locus_tag="AC16BC_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HIR3240701.1"
                     /translation="HLFVLFAFHLSTAKPLRVYPPGQTRERGVFATRSPHRPNGIGIL
                     TVELLRREGNRLFVKGLDVVDGTPVLDIKPYLGHFDCRPQATLGWMPDKPDAGQF"
     gene            362..625
                     /locus_tag="AC16BC_03435"
     CDS             362..625
                     /locus_tag="AC16BC_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR3240702.1"
                     /translation="MFGGVGIYLDRVFFALIANDVLYFKVDDSNRADYETSGMTPFMP
                     HPTRRTTMPYYEVPASVLEDVDQLTAWGRKAYSVASRNRSRGS"
     gene            complement(898..1986)
                     /locus_tag="AC16BC_03440"
     CDS             complement(898..1986)
                     /locus_tag="AC16BC_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3240703.1"
                     /translation="MNGIAGRVALAAVAVTALIATVAGGCVHVLQPPAFDDAAWMRDS
                     RASARATLYAPHERDGRFFNPWRPDDRGFLTLLRWRLSERTRAYPDPVPPVDVVANDG
                     AYLNRSREPASLTWIGHATFAIQINDQVWVTDPMFSDRAVLPKRAAPPGIPISAIPEG
                     AFALISHNHYDHLDAPSVAALPPSTRWFVPKGLGPYVRHAGATTVEELDWWDRVEVGD
                     TTLICLPAQHWSNRLDMGRNRSLWAAWLLITPTRKIYFGGDSGSFHGFAEIGERFGPI
                     DDAIIPIGAYEPRWFMAYSHMDIDESLRAFEALRATRMIPMQFGIFQLGDEPAAFPAW
                     ELRRALDARSDLAARVHIPAVGARLMMD"
     gene            complement(1983..>2654)
                     /locus_tag="AC16BC_03445"
     CDS             complement(1983..>2654)
                     /locus_tag="AC16BC_03445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3240704.1"
                     /translation="YLPNPAARYTVLDFHGNGGNLGDRVTLYARWHCLGLAVFAIDYR
                     GYGRSQGKPSEQGLYRDARAAWSDLTGDLAVPPDRIVVAGRSLGSGPAVQLATEVRPA
                     GLVLESPMTNIPDMARVVYPFLPVSLLVRTRFDNAQKIGRVTCPVLVVHAGQDDIIPP
                     AMGRRIFETARDPKHWALLPGRHNDFDDVSTDAYLAAWRSFLDSLPVQEPIPPPSTLS
                     NGGDE"
BASE COUNT          441 a          864 c          914 g          435 t
ORIGIN      
        1 cgcacctgtt cgtgctgttt gcctttcatc tgagcacggc gaagccgttg cgcgtgtacc
       61 cgccgggcca gacccgcgag cggggcgtgt tcgccacgcg ttcgccgcat cggcccaacg
      121 ggatcgggat tctgaccgtg gaactcctca gacgcgaggg gaatcgtttg ttcgtcaaag
      181 gtctcgacgt ggtggacggc acgccggtgc tcgatatcaa accgtatctg ggacatttcg
      241 actgccgccc tcaagccacg ctggggtgga tgccggacaa gcccgatgcc ggtcagttct
      301 gagtatcgcg actacgtgct cgaaacagct cgcgtgcctc gggagcgtca cgggtcgccc
      361 gatgttcggc ggcgtgggga tctacctcga tcgggtgttc ttcgcgttga tcgcgaacga
      421 cgtcctgtac ttcaaagtcg atgactcgaa tcgggccgac tatgaaacct cggggatgac
      481 gccgttcatg cctcatccca cccgacgcac caccatgccg tactacgaag tccccgcgtc
      541 ggtgcttgag gacgtcgatc agttgaccgc gtggggccgc aaggcctatt cggtcgcctc
      601 acgcaatcgc tcacgcggtt cctaggaacc ggtacgagtc atcccggttg gagcctgttc
      661 agggaaggtc agatgccggg cgaacccgcc ttgcgccaac ggcgcaaaat acaaacggcg
      721 tggtcctctc gggtggcgaa gcaagggagg accttgcgcg cggagcgtac tgggtcgtac
      781 gtgagcacgc aaggccgacc gacaacgccg cagatggcct ttgcttcgcc acctgcaagg
      841 tgcctgcggc cgaatcagct tcgcgccttc ggcgcgcgca ggttcatgga caggctccta
      901 atccatcatc aggcgcgcgc ccaccgcggg aatgtgcacc cgggccgcca ggtccgaccg
      961 cgcgtccagg gcgcggcgca gttcccacgc ggggaacgcg gcgggttcgt cgccgagctg
     1021 aaagatcccg aactgcatcg ggatcatgcg cgtggcgcgc agagcctcga acgcgcgcaa
     1081 cgattcgtcg atgtccatgt ggctgtacgc catgaaccag cgcggctcgt acgcgccgat
     1141 cggaatgatc gcgtcgtcga tgggcccgaa ccgctcgccg atctccgcga aaccgtgaaa
     1201 ggaccccgag tccccgccga agtaaatctt ccgagtcggg gtgatgagca gccacgcggc
     1261 ccacaagctg cgattcctgc ccatgtcgag gcgattggac cagtgctgcg cggggaggca
     1321 gatgagcgtg gtgtcgccca cttccacccg gtcccaccaa tcgagctcct ccacggtggt
     1381 cgcgccggcg tgtcggacgt atggaccgag ccctttcggc acgaaccagc gcgtgctcgg
     1441 gggaagcgcg gccacgctcg gcgcgtccag gtggtcgtag tggttgtgcg agatcagggc
     1501 aaacgcgccc tcggggatcg cggaaatcgg gatgcccggc ggcgccgcgc gtttgggcag
     1561 gaccgcgcga tcgctgaaca tggggtccgt cacccacacc tggtcgttga tctggatggc
     1621 gaacgtggcg tggccgatcc atgtgaggga tgcgggttcg cggctgcggt tcagatacgc
     1681 gccgtcgttg gccaccacgt ccaccggcgg cacggggtcg gggtacgcgc gcgtgcgctc
     1741 cgagagccgc cagcgcagga gcgtcaggaa tcctcggtcg tcgggtcgcc acggattgaa
     1801 aaaccggccg tcgcgttcat gcggcgcgta cagcgtcgcg cgcgcggacg cgcgtgagtc
     1861 gcgcatccag gccgcgtcgt cgaacgcggg gggctgaagg acgtggacgc agccgccggc
     1921 cacggtggcg atcagcgcgg tcaccgccac ggccgccaac gccacgcggc ccgcgatccc
     1981 gttcactcgt ctcccccgtt tgacaacgtg gagggagggg gaatcggctc ctggaccggc
     2041 aacgagtcga ggaacgacct ccacgccgcc aggtacgcgt cggtcgagac gtcgtcaaaa
     2101 tcattgtgac ggcccggcag caacgcccag tgcttggggt cgcgcgcggt ctcgaatatt
     2161 cggcggccca tggcgggcgg gatgatgtcg tcctgtcccg cgtggacgac gagcacgggg
     2221 cacgtcacgc gtccgatttt ttgcgcattg tcgaaccggg ttcgcaccaa cagcgagacc
     2281 ggcaggaacg ggtagaccac gcgcgccatg tcgggaatgt tggtcatggg gctttccagc
     2341 accaggcccg cgggccggac ttcggtggcg agttggaccg cgggaccgct gcccagactc
     2401 cggcccgcca ccacgatccg gtcgggtggg acggcgaggt cgccggtgag gtcgctccag
     2461 gcggcgcgcg cgtcgcggta caacccctgt tcggacggct tgccctggct ccggccgtac
     2521 ccgcggtaat cgatcgcgaa cacggccaat cccaggcagt gccaccgcgc gtagagcgtc
     2581 acacggtcgc cgagattgcc gccgtttccg tggaaatcga gcacggtata ccgcgcggcg
     2641 gggttcggca ggta
//
LOCUS       DBNGTT010000151         4486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1308194, whole genome shotgun sequence.
ACCESSION   DBNGTT010000151 DBNGTT010000000
VERSION     DBNGTT010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1308194"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2473)
                     /gene="rpoC"
                     /locus_tag="AC16BC_03450"
     CDS             complement(<1..2473)
                     /gene="rpoC"
                     /locus_tag="AC16BC_03450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3240705.1"
                     /translation="MEGLYNFFEKPKDTISFDAIRIRVASPEKIRSWSYGEVKKPETI
                     NYRSFKPERDGLFCAKIFGPIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERM
                     GHIELASPVAHIWFLKGVPSRIGTLLDMTLKHLERILYFESYVVIEPGEAPVKEKELV
                     TEDRYRQLMQEHGDTFRAEMGAEAIRELLAKIELEGHYAELQAKIGQASSAAVKKKLT
                     KRLKVIEAFRKSGNKPEWMIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPAVIIRNEKRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKKMALELFKPFIFQKLEQRGYVT
                     TIKSAKKMVERERPEVWDVLDEVIKEHPILLNRAPTLHRLGIQAFDPILIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSVEAQIEARVLMMSINNILSPANGKPIMTPSQDMVL
                     GCYWLTKERAGSKGQGKVFYGPEEVRVAYDAGEVEEHARIKVRRDGALVETTVGRVIL
                     GEILPTQLPFSAVNRELTKKELMKLIDLCYRGAGHRETVILLDRIKSIGYQYATRAGL
                     SICIDDMHIPTQKESMIGKAKKEVAEIEKQYSEGLITNGERYNKVVDIWAHTTEQVAS
                     EMMKELGEEEESIKKGTDVVLQTKRRDRQQGQAADGERRDFNSIFMMADSGARGSAQQ
                     IRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSG
                     YLTRRLVDVAQDAIITEDDCGTLDGI"
     gene            complement(2675..>4486)
                     /gene="rpoB"
                     /locus_tag="AC16BC_03455"
     CDS             complement(2675..>4486)
                     /gene="rpoB"
                     /locus_tag="AC16BC_03455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3240706.1"
                     /translation="GYVVLAQRSGVVESVDATRIVVRSDVGKKAAEDEDGDAGADVYR
                     LIKCQRSNQNTCINQKPVVRVGQRVKRGQVLADGPAIDHGELALGRNVLVAFMPWGGY
                     NFEDAILVSEALVREDQFTSIHIEEFELESRDTKLGKEEITRDIPNVSEEALRNLDES
                     GIIRIGAEVRPGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVKDASLYVPPGIEG
                     VIVDVKIFSRKGVDKDERAKTIESEDIHRLQRVHQDEIRILEEETIKKVRRYLVGKTV
                     AKDLVDRDSGEVILKKKKTITKEVIDALSDDDLRNIVLSEPSEQDRIEELQRTAKEQI
                     DVLQTAYDEKIGRLKKGDELPPGVIKLVKVYVAMKRKLQVGDKMAGRHGNKGVVARVM
                     PVEDMPYLPDGRPIEIVLNPLGVPSRMNVGQILEIHLGWAARALGLHVSSPVFDGATE
                     KEIKELLSQANLPTSGQTTLFDGRSGEPFKRPVTVGYMYMLKLHHLVDDKIHARSIGP
                     YSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASTLQEFLTVKSDDVPGRSRIYEAI
                     VKGENFLEPGLPESFNVLIKELQSLGLDVELIKKEKD"
BASE COUNT          717 a         1504 c         1306 g          959 t
ORIGIN      
        1 tgatgccgtc gagggtcccg cagtcgtcct cggtaatgat cgcatcctgg gcgacgtcga
       61 ccagacggcg agtcaggtaa ccggagttgg cggtcttcag cgccgtgtcg gccaaaccct
      121 tgcgggcacc gtgagtcgag atgaagtact ggagaacggt caagccctcg cggaagttgg
      181 cggtaatcgg cgtctcgatg atttctcccg acggcttggc catcaggccg cgcatcccgc
      241 cgagctgccg gatctgctgg gcgctgccgc gcgccccgga gtcggccatc atgaagatcg
      301 agttgaaatc gcgccgctct ccatcggcgg cttgaccctg ctgccgatcg cgtcgcttcg
      361 tctggaggac cacatccgtg cccttcttga tgctctcctc ctcctcgccc agctccttca
      421 tcatctcgct cgccacctgt tcggtggtgt gcgcccaaat gtccacgacc ttgttgtagc
      481 gctcgccgtt ggtgatcaac ccttcggagt actgcttctc gatctccgcg acctctttct
      541 tggctttccc gatcatcgac tccttttgcg tcgggatgtg catgtcgtcg atgcagatcg
      601 acagcccggc gcgggtcgcg tactgatagc cgatgctctt gatgcggtcc agaaggatca
      661 cggtttcgcg gtgccccgcg ccccggtaac acaggtcgat gagcttcatc aactcttttt
      721 tagtcaactc gcggttgacg gccgagaacg gcagctgcgt cggcaggatc tcgccgagga
      781 taactcgacc caccgtggtc tcgaccaacg cgccgtctcg acgcaccttg atccgcgcgt
      841 gctcctccac ctcgccggcg tcgtaggcca cccgcacttc ctcgggtccg tagaacactt
      901 tgccctggcc cttggacccg gcgcgttcct tggtcagcca gtagcagcca agcaccatgt
      961 cctgcgacgg ggtcatgatc ggcttgccgt tcgcgggcga cagaatgttg ttgatggaca
     1021 tcatcagaac gcgcgcctcg atctgcgctt ccaccgagag cggcacgtgc accgccatct
     1081 ggtcgccgtc gaagtccgcg ttgaacgcgg cgcagaccaa cgggtgcaac tggatcgcct
     1141 tgccctcgat caggatcgga tcgaacgcct gaatacccag gcggtgcaag gtgggggcgc
     1201 ggttgagcag gatcgggtgc tccttgatca cttcatccag cacgtcccac acctcgggcc
     1261 gttcgcgctc caccatcttc ttggcgctct tgatcgtggt gacgtagccg cgctgttcca
     1321 gcttctggaa aatgaacggt ttgaacagct ccaacgccat cttcttgggc aatccgcact
     1381 ggtgcaaccg gagctccggc cccaccacga tcacggaccg gcccgaatag tccacgcgtt
     1441 tgccgagcag gttctgacgg aaccgccctt gcttgccctt gagcatgtcg gacaaggact
     1501 tgagcggccg cttgttcgga ccgcggatcg cccggccgcg ccgcccgttg tcgaagagcg
     1561 cgtccaccgc ttcctgcaac atgcgcttct cgttgcggat gatcacggcc ggcgccttca
     1621 gttccagcaa ccgcttgaga cggttgttgc ggttgatcac gcgccgatac aggtcgttga
     1681 ggtcggacgt cgcgaagcgg ccgccgtcca gcggcaccaa cggccgcaat tcgggcggca
     1741 acaccgggat cacgtccatg atcatccact cgggcttgtt gccggacttg cggaacgctt
     1801 cgatgacttt gagccgcttg gtcagcttct tcttgaccgc cgcggagctc gcctgcccga
     1861 tcttggcctg cagctcggcg tagtgtcctt ccagttcgat cttggccagg agttcgcgga
     1921 tcgcttccgc gcccatctcg gcccggaacg tgtcgccgtg ctcctgcatc aactggcgat
     1981 atcggtcttc ggtgacgagt tccttttcct tgaccggcgc ctcgcccggc tcgatgacca
     2041 cgtagctctc gaagtacaag atccgctcaa ggtgcttgag ggtcatgtcg agcaaggttc
     2101 cgatccggct gggcacgccc ttcaagaacc agatgtgcgc gaccggcgaa gccagttcga
     2161 tgtggcccat gcgttcgcgg cggaccttgg attggatgac ctcgacgccg cacttgtcgc
     2221 acacgatccc gcggtgcttc atccgcttgt atttgccgca gttgcactcc cagtccttga
     2281 tcggaccgaa gatcttcgcg cagaacagcc cgtcccgctc cggcttgaac gaccggtagt
     2341 tgatggtctc gggctttttg acttccccgt acgaccacga gcgaatcttc tcgggcgacg
     2401 cgacccggat gcgaatcgca tcgaacgaga tggtgtcttt gggcttctcg aaaaagttat
     2461 agagtccttc cacgttcccc ccctttagtg agcggttagc tcacagctca taaggaaccg
     2521 ccacgggcac ctgactgagc aaccagatcc cgggcctgtt caggagcgcg ccagatgcaa
     2581 ggcgccgctg agcaccggac cggagcgtac tccgtacgta cgtgaggacc ggagcacaag
     2641 cggcaacgcc gcagatggcc gttcatggac cggcctaatc cttctctttc ttgatcaatt
     2701 ccacatcaag cccgaggctc tgcaactcct tgatgagcac gttgaacgac tcggggagcc
     2761 cgggctccag gaagttttcg cctttgacga ttgcttcgta gatgcgggaa cgccccggca
     2821 cgtcgtcgga cttgaccgtc aggaactcct gcagggtgga agccgcgccg tacgcttgca
     2881 gcgcccagac ttccatttcc ccgagacgtt gcccgccgaa ctgcgccttg ccgcccagcg
     2941 gctgctgcgt caccaacgaa tacggcccga tcgagcgggc gtggatcttg tcgtccacca
     3001 agtgatggag cttcaacatg tacatgtagc ccacggtcac cggccgcttg aacggctcgc
     3061 cgctgcgacc gtcgaacagc gtggtttgac ccgacgtggg caggttcgcc tggctcagga
     3121 gctccttgat ctccttttcc gtggccccgt cgaacaccgg agaactcacg tgcaggccca
     3181 acgcccgcgc cgcccagccc aggtggatct ccaggatctg gccgacgttc atacgcgagg
     3241 gcacgcccaa cgggttcagc acgatttcga tcgggcgacc gtccggcagg tacggcatat
     3301 cctcgaccgg catcacgcgc gccaccacgc ccttgttgcc gtgacggccc gccatcttgt
     3361 cgcccacctg cagtttgcgc ttcatggcga cgtacacctt gaccagcttg atcacgccgg
     3421 gcgggagctc gtcgcctttc ttgaggcggc cgatcttctc gtcgtacgcg gtctggagca
     3481 cgtcgatctg ctccttggcc gtccgttgca gctcttcgat ccggtcctgc tcggacggtt
     3541 cgctcagcac gatgttgcgg aggtcgtcgt cgctcagcgc gtcgatcacc tctttggtga
     3601 tggtcttctt ctttttgagg atgacttcac cgctgtcgcg gtccaccagg tccttggcca
     3661 cggtcttacc gaccagataa cgccggactt tcttgatggt ttcttcctca agaatccgga
     3721 tttcgtcctg gtgcacgcgt tgcaggcgat ggatgtcctc gctctcgatc gttttcgcgc
     3781 gctcgtcctt gtccacgccc ttgcgcgaga agatcttcac gtccacgatc acgccttcga
     3841 tcccgggcgg cacgtagagc gacgcgtcct tcacgtcgcc ggccttttcg ccgaaaatcg
     3901 cgcgcaacag tttttcctcg ggcgtgagct gggtctcgcc cttgggcgtc accttgccga
     3961 ccaggatgtc gccgggacgg acttcagccc cgatgcggat gatgccgctc tcgtcgaggt
     4021 tgcggagcgc ttcctcgctg acgttgggga tgtcccgggt gatctcttcc ttgccgagtt
     4081 tggtgtcgcg ggattcgagc tcgaattcct cgatgtggat cgacgtgaac tgatcctccc
     4141 ggaccaaggc ctcgctgacc aggatggcgt cttcgaagtt gtacccgccc cacggcatga
     4201 acgcgaccag gacgttgcgg cccaacgcca actcgccgtg gtcgatcgcg ggaccatcgg
     4261 ccaacacctg gcccctcttg acccgctgcc cgacccgcac caccggcttt tggttgatgc
     4321 aggtgttctg gttggaccgc tggcatttga tcagccgata cacgtcggcg cccgcgtctc
     4381 cgtcctcgtc ctccgccgcc ttcttgccga cgtcggaccg gaccacgatc cgggtggcgt
     4441 ccacgctctc caccacgccc gaccgctgcg ccaggacgac gtaccc
//
LOCUS       DBNGTT010000152         2387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1308206, whole genome shotgun sequence.
ACCESSION   DBNGTT010000152 DBNGTT010000000
VERSION     DBNGTT010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1308206"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="AC16BC_03460"
     CDS             complement(<1..215)
                     /locus_tag="AC16BC_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR3240707.1"
                     /translation="MTTGDLDDVLVIERASFSKPWTRTMFLAELSENPCARFFVAVTG
                     AELVGYIGCWVVAVEMQVVNLAVRMDA"
     gene            complement(280..1038)
                     /gene="tsaB"
                     /locus_tag="AC16BC_03465"
     CDS             complement(280..1038)
                     /gene="tsaB"
                     /locus_tag="AC16BC_03465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3240708.1"
                     /translation="MKWLAIDTSLDAVSAAVWEGEGVPGHGTVGEVVCPERGAGSRDL
                     LPAIDRLLAGHGWSVANLDGVAVTIGPGSFTGLRIGVSLVKGWAAARPLRVIAVGTLD
                     AVAAASGQSGLVAALVDAGRGEVYARVFDCRDGGVVPMGEEALALPDAWAATLPGDEP
                     LWCVGSGAVRHADALRAALGARAVMLSDLKTSAAAGALRLGVPRLLAGEETPVGELLP
                     RYLRRSTAETNWERGVVGSRRRKLLGWDPAHGRT"
     gene            complement(1049..1792)
                     /locus_tag="AC16BC_03470"
     CDS             complement(1049..1792)
                     /locus_tag="AC16BC_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240709.1"
                     /translation="MTSRWLLVAAAFWVALLSGCASHRHVVETESLPKREATLAELER
                     ILAERSQVGGYRALVTFEVSRGGRSGSVKGTVRFAPPRTYEVRGLDPLGRDVFALNAE
                     GEHITLRRADGVPALNGSDAIDAELAPWIGSLRVADVLRVVGAGQGPSVDPLDMLALE
                     RGEDYYTLYVLVFDDGRARLDRKIRFERTRFLAVSEEWFDVNGSRRLRVEFDRYREVG
                     GQWRPFLVSASNGAGTLKIDFHEVAAEPS"
     gene            complement(1833..>2387)
                     /locus_tag="AC16BC_03475"
     CDS             complement(1833..>2387)
                     /locus_tag="AC16BC_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR3240710.1"
                     /translation="LSEVANPSELFLAERSHGTSGSAILASAEGTRPILIELQALVTP
                     PAAGTPRRSANGIEPARLGLLLAVLEKRVGLGVGAQDVFVNVAGGLSLDEPAADLAVA
                     AAVASSWTDRALDPKTVVFGEVGLAGEIRAVPQADIRIREAAALGFARCVMPRRNADR
                     AEASGVSVIGVEDVHEALEALGIR"
BASE COUNT          409 a          952 c          711 g          315 t
ORIGIN      
        1 cacgcgtcca tgcgcaccgc caggttcacg acctgcattt caaccgcgac cacccagcac
       61 ccgatatagc ccaccagctc tgcgccggtc accgccacga aaaatcgcgc gcacgggttc
      121 tcggacaact cggccaagaa catcgtccgg gtccagggtt ttgaaaacga cgcgcgctcg
      181 atcacgagca cgtcgtccag atcacccgtc gtcatggggc gaacgtcaac gcgcaaatca
      241 gcgacggatc gagacccggt ccgatcaccc atcaatgcgc tacgtccgac catgcgctgg
      301 atcccagccc aagagtttcc gtcgccgact ccccaccacg ccgcgctccc agttcgtctc
      361 ggccgtggac cggcggaggt agcgcgggag caactccccg accggcgtct cctctcccgc
      421 gagcagccgc ggaacgccca gacgcaacgc ccccgcggcc gcgctcgtct tcagatcgga
      481 cagcatcacg gcgcgcgcgc ccagggccgc gcgcagcgcg tccgcgtgcc gcaccgcgcc
      541 cgagcccacg caccacaggg gttcatcgcc cgggagcgtc gcggcccacg cgtccggcaa
      601 cgccagcgct tcttctccca tcggaacgac gccgccgtct cgacagtcga acacgcgcgc
      661 gtagacctca ccgcgacccg catcgaccag cgccgcgacc agacctgatt gtcccgacgc
      721 cgcggccacg gcatccaacg tgcccaccgc gatcacgcgc aacggccgcg ccgcggccca
      781 ccctttgacc agactgacgc cgatgcgaag cccggtgaac gagccgggac cgatcgtcac
      841 cgccacccca tccagattcg ccacggacca cccgtggccc gcgagcaatc ggtcaatggc
      901 cggcaacagg tcgcgcgagc ccgcgccgcg ctcgggacag accacttcac cgaccgtccc
      961 gtggcccggt acgccctcgc cctcccacac cgcggcgctc accgcgtcca gcgaggtatc
     1021 gatggccaac cacttcatcc cgatcgcctc acgacggctc cgccgcgact tcgtgaaagt
     1081 cgattttcag cgtgcccgcc ccgttgctgg cgctcacgag gaacggccgc cactgtccgc
     1141 ccacttcgcg gtaccggtcg aactccaccc gcagccgtcg ggacccgttt acgtcgaacc
     1201 actcctcgct cacggccagg aaccgcgtgc gttcgaaccg gattttccga tccagacgcg
     1261 cccggccgtc atcgaacacg agcacgtaca gcgtgtaata gtcctccccc cgttccagcg
     1321 cgagcatgtc cagcgggtcc accgacggtc cctgccccgc gcccaccacc cggagcacgt
     1381 ccgcaacgcg cagcgacccg atccacggag cgagttcggc gtcgatcgcg tcggacccgt
     1441 tgagcgcggg gacgccgtcg gctcgccgga gcgtgatgtg ttcgccctcg gcgttcagcg
     1501 cgaacacgtc ccggcccaac ggatcgaggc cgcgaacttc gtacgtgcgc ggcggggcaa
     1561 accgaaccgt gcccttcacg ctgccggagc gaccgccgcg cgatacctcg aaggtgacca
     1621 gcgcccgata gccgcccacc tgcgatcgct cggccaatat ccgttcgagc tccgcgagcg
     1681 tggcctcgcg cttgggcagc gattccgtct ccaccacgtg gcggtgggac gcgcagccgc
     1741 tgagcaacgc cacccagaag gccgccgcca cgagcaacca tcggctcgtc atgcggcgga
     1801 cacccacggt cggacttgtg gcgaacgacg tttcagcgaa tgcccaaggc ctcgagcgct
     1861 tcatgcacgt cctcgactcc gatgaccgac acgcccgagg cctcggcccg atcggcgttg
     1921 cgacgcggca tcacgcagcg ggcaaaaccc aacgcggcgg cttctcggat gcgaatgtcc
     1981 gcctgcggca ccgcgcggat ttcccccgcc agaccgactt caccgaacac cacggttttc
     2041 gggtcgagcg cgcggtccgt ccaactcgag gccacggccg cggccacggc cagatccgcc
     2101 gcgggttcgt cgagcgacaa tccgcccgcc acgttgacga acacgtcttg cgcgcccacg
     2161 cccaggccca cgcgtttctc gagcacggcc agcaacaggc ccaaccgcgc gggctcgatc
     2221 ccgttggccg accgtcgcgg ggtcccggcc gccggcggcg tgaccagggc ctgcagttcg
     2281 atcaggatcg gccgcgtgcc ttcggcgctg gccaggatcg cggagccgga cgtgccgtgc
     2341 gagcgctcgg ccaagaacaa ctccgagggg ttggccacct cggacag
//
LOCUS       DBNGTT010000153         2544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1052574, whole genome shotgun sequence.
ACCESSION   DBNGTT010000153 DBNGTT010000000
VERSION     DBNGTT010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1052574"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="AC16BC_03480"
     CDS             complement(<1..889)
                     /locus_tag="AC16BC_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR3240711.1"
                     /translation="MPLATLRRLSAPRMTFALAVLAAVTACSPAPRSQGKPVWTNFET
                     GANVKSIAIDGRYLWLGLANGLIRYDSATHDSHEIYTPERTNGGLLSNGVYAVRRDPS
                     GRVWVGTYGGGLSAFDGSVWTTFTPYGAGHPATYGANWTRYPRGRGLGDLWVYDVAFD
                     GKGATWVATWKGLSRFDGADCFPLGASSATPTPPCGFVTYTVDDGLVDKWVYAIAVDR
                     DGSLWLGTEGGVNRFDGAAWAAYTHRDGLGADPTTLPSKTAGEAPSPHHTTAYKNIGQ
                     ANPNYVLAIAIDRTGAKWFG"
     gene            1016..1088
                     /locus_tag="AC16BC_03485"
     tRNA            1016..1088
                     /locus_tag="AC16BC_03485"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1049..1051,aa:Val,seq:tac)
     gene            1205..1281
                     /locus_tag="AC16BC_03490"
     tRNA            1205..1281
                     /locus_tag="AC16BC_03490"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1239..1241,aa:Asp,seq:gtc)
     gene            1487..2188
                     /locus_tag="AC16BC_03495"
     CDS             1487..2188
                     /locus_tag="AC16BC_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3240712.1"
                     /translation="MNSSIDLSDLITSASPVSQAVLAVLLALSIFSWAVILYKWRLFR
                     VVRAQDRRFLGVYEALDDPTEVRRRSATIARSPMAELFAIAHRTALSDRSVEPAGGGP
                     SVRRLERALRQASERHLMAEEEYLSALATVGNVSPFVGLFGTVLGIIQAFQSIGRQGT
                     ASIAAVAPGVAEALVATAAGLFAAIPAVVAYNYFLTQVRRHASQLDVFRQDLLGLVES
                     RVPSASTLPETSGRL"
     gene            2192..>2544
                     /locus_tag="AC16BC_03500"
     CDS             2192..>2544
                     /locus_tag="AC16BC_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3240713.1"
                     /translation="MWADRRRTDPGRLLAEINVVPLVDVVLVLLVIFMITAPLMLRGI
                     DIDLPKSSVNTIKPEERMMLTVSRDRAVYFDKERIARVSLDEKLTRIAREKPDVTLYL
                     RADRDVPYGAVVEVM"
BASE COUNT          391 a          816 c          870 g          467 t
ORIGIN      
        1 tcccgaacca cttggctccg gttcggtcga tcgcgatcgc caggacgtaa ttggggttgg
       61 cctgcccgat attcttatac gcggtcgtgt ggtgcgggct gggcgcttcg cccgcggtct
      121 tggacggcag cgtggttggg tcggcgccga gcccgtcgcg gtgcgtgtag gcggcccacg
      181 cggcgccgtc gaatcggttc acgccccctt cggtgccgag ccagagcgag ccgtcgcgat
      241 cgaccgcgat cgcgtacacc cacttgtcca cgaggccgtc atcgaccgtg tacgtgacga
      301 acccgcaagg cggtgtgggc gtcgcggatg acgcgccgag cggaaaacag tcggcgccgt
      361 cgaaccggct caatcccttc caggtcgcca cccaggtagc gcccttgccg tcgaacgcca
      421 cgtcgtacac ccacaaatcg ccgagcccgc ggccgcgcgg atagcgggtc caattcgcgc
      481 cgtaggtggc ggggtgaccc gcgccatagg gcgtgaacgt ggtccagacc gatccgtcga
      541 acgcggacag cccgccgccg tacgtgccga cccagacgcg gccgctcgga tcgcggcgca
      601 cggcgtagac cccgttgctg agcaacccgc cgttggtgcg ctccggcgtg tagatctcgt
      661 gcgagtcgtg ggtggccgag tcgtatctga tcaggccgtt ggccagtccc agccagagat
      721 agcggccgtc aatggcgatg gacttcacgt tcgcgccggt ttcaaagttc gtccacaccg
      781 gttttccctg ggagcgcggc gcgggcgaac aggcggtaac cgcggcgagg accgccaggg
      841 cgaacgtcat ccgcggggcc gagaggcggc gtagcgtggc gagtggcatc aggcagaaca
      901 gtccgcgtcg atcgcgtgtg cgtggcgtgc tcgttgacaa tgcgcgagtc aggttgctat
      961 agtctacgcg gtttttcgac gcgtcgcaac gcggcgagcc gtgaacgcat cccacgggcg
     1021 gatagctcaa ttgggagagc gctgccctta caaggcagag gtcaggcgtt cgatccgcct
     1081 tccgcctatg gtcgcgcgaa aggcgagcag tcagctcaca cgcgaccgct cacggggaga
     1141 cgaaccgaca ggcgtcgggg gtgttccgtg agcggtcgcg tgtgagctgt gagctatgat
     1201 gtgcggggcc gtagttcagt tggttagaac gccggcctgt cacgccggag gtcacgggtt
     1261 cgagccccgc cggccccgcc attcctcgct tgcgaagcgc aacgtagggc ccgttttccc
     1321 ggcccgaggc tgcgagacgt tgttgaattt cgccgtgctc ttatcctcgc ttgcgaagcg
     1381 caacataggc gcgctttccc gcgacgaggc tgcgagatgc gtttgacgtt cgcagcgtct
     1441 tcggtaagtg tagtccgtat gccttccgtc cactccgaga ccccgcatga attcttcgat
     1501 cgatctgagt gatctcatta cgtccgcgag cccggtgagt caagcggtcc tcgcggtgct
     1561 cctcgcgctc tcgatttttt cctgggccgt gatcctgtat aaatggcggc tctttcgcgt
     1621 ggtgcgcgcc caggatcggc gatttctggg cgtgtacgag gcgctcgacg accccaccga
     1681 ggtgcggcgc cgcagcgcga ccatcgcccg cagtcccatg gccgaactgt tcgcgatcgc
     1741 gcaccgcacc gcgttgagcg acaggagcgt cgagccggcg ggcggcgggc ccagcgtccg
     1801 ccggttggag cgcgcgctcc ggcaagcgtc ggaacgccat ctcatggccg aggaggagta
     1861 cctgtccgcg ctcgccacgg tcgggaacgt gtcgccgttc gtgggactct tcggcaccgt
     1921 gctcggcatc atccaggcgt ttcaatcgat cggccgacaa ggcaccgcga gcatcgcggc
     1981 ggtggcgccc ggggtggcgg aggcgctggt ggcgaccgcg gccgggttgt tcgccgcgat
     2041 tcccgccgtg gtggcctaca attattttct gacccaggtg cggcgccacg cgagccaact
     2101 ggacgtgttc cgccaggatc tcctcggcct ggtggagtcg cgcgttccgt ccgcctcgac
     2161 gcttcctgaa acgtcgggtc gactgtgagc catgtgggct gaccgacgcc gcaccgatcc
     2221 gggacgactt ctcgccgaga tcaacgtggt gccgctcgtt gacgtggtgc tcgtcctgct
     2281 ggtgatcttc atgatcacgg ctccgctcat gttgcgcggc atcgatatcg acctccccaa
     2341 gtcgtcggtg aacaccatca agccggagga acggatgatg ctcaccgtgt cgcgcgatcg
     2401 ggcggtctat ttcgacaagg aacgcatagc ccgcgtgtcg ttggacgaga agttgacccg
     2461 gatcgcccgc gaaaaacccg acgtgacgtt gtacctgcgg gcggatcgcg acgtgcccta
     2521 cggcgcggtg gtcgaggtga tgga
//
LOCUS       DBNGTT010000154         1882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1308595, whole genome shotgun sequence.
ACCESSION   DBNGTT010000154 DBNGTT010000000
VERSION     DBNGTT010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1308595"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1238
                     /locus_tag="AC16BC_03505"
     CDS             240..1238
                     /locus_tag="AC16BC_03505"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3240714.1"
                     /translation="MTAISVKGLNKTFDYFKKEPGLKGSLKSLFWRETLYHHAVKDVS
                     FAIEEGELVGFLGPNGAGKTTTLKILSGILYPTSGEVSVLGHVPWKREPAYQRQFAIV
                     MGQKNQLWWDLPARESFELNRDIYEIDRSAYQRTLNELVDILGVGQLLDVQVRKLSMG
                     ERMKCELIAALLHQPRVLFLDEPTIGLDVVSQENIRAFIDAYNRTNKTTVILTSHYMR
                     DVERLCRRVLVISDGRIIYDGSLAELADRYVDHKIVTLRLTQPVAPQALARFGEIAEH
                     NGLSVTLNVPKKDLAQTAQGLLSALPVEDLSIEEVAIESVIATMFAEQSGGERETN"
     gene            1225..>1882
                     /locus_tag="AC16BC_03510"
     CDS             1225..>1882
                     /locus_tag="AC16BC_03510"
                     /inference="COORDINATES: protein motif:HMM:NF020028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR3240715.1"
                     /translation="MKRIDVPSWSLADLYAGPDDPGLTREVTETLAQADAFERRYRGR
                     VAPGTLSPAELAEAVAQLEAMNERLRKIGTYASLLFAADMGVPRHGALYQASQEQGTA
                     LRQRLTFFDLEWSAVPEARAKDYLAAPELSRYRHYLEIERRLAPYRLSEPEELVLETK
                     ANTGAHAFQRLFDEVLARARFDVVVGRKRERLSEQETLARLHAPDRATRKAAARGLTA
                     G"
BASE COUNT          360 a          569 c          631 g          322 t
ORIGIN      
        1 ggaatgatga ccctggcgac gctggtgtgg aacgtcagtt tgcgccacta ctcgtcggcg
       61 agctcgtagg catagatcac agtgaccact caccgtacac gacgtcaata caccccccac
      121 ccacccctcc ccctcaaggg gggaggaacc gaaatggtca ccctccccct tgacggggga
      181 gggcaaggga gggggtggcc tttagttgtc gttcccgtaa accgcatgcg tggccccgca
      241 tgactgccat cagcgttaaa ggcctcaaca agaccttcga ctactttaag aaggagcccg
      301 ggctcaaggg ctctctaaag agtctgttct ggcgggagac gttgtatcac catgcggtca
      361 aggacgtgtc gttcgccatc gaggaaggcg agttggtcgg gtttctgggg cccaacggcg
      421 cgggcaagac caccacgctc aagatcctgt ccgggattct ctatccgacg tctggcgaag
      481 tctcggtgct gggccacgtg ccgtggaagc gcgagcccgc gtaccagcgg caattcgcca
      541 ttgtcatggg gcaaaagaac caactgtggt gggatctgcc tgcgcgcgaa tcattcgaac
      601 tcaaccgcga tatctatgaa atcgaccggt ccgcgtacca acggacgttg aacgaactgg
      661 tggacatcct cggggtcggc cagctcctcg acgtgcaggt ccggaaattg tcgatgggcg
      721 agcggatgaa gtgcgaactg atcgcggcgc tgctgcacca gccgcgcgtg ctgttcctgg
      781 acgaacccac gatcggcttg gacgtggtgt ctcaggagaa catccgggcg ttcattgacg
      841 cctacaaccg caccaacaag accaccgtga tcctgaccag ccactacatg cgcgacgtgg
      901 agcggctgtg ccggcgcgtg ctggtgatca gcgacggacg gatcatctac gacgggtcgt
      961 tggccgagtt ggccgaccgg tacgtggacc ataagatcgt gacgttgcgc ttgacgcagc
     1021 cggtggcgcc gcaggcgctc gcgcggttcg gagaaattgc cgagcacaac ggattgagcg
     1081 tgaccctgaa cgtgcccaag aaagatctgg cccagaccgc gcaaggcctc ctctcggcgc
     1141 tgccggtcga ggatttgtcg atcgaagagg tggcgatcga atccgtgatc gccacgatgt
     1201 tcgcggaaca atccgggggg gaacgtgaaa cgaattgacg tgccgtcgtg gagtttggct
     1261 gatctgtacg ccgggccgga tgacccggga ttgacgcgcg aggtgacgga gaccttggcg
     1321 caggccgacg cgtttgagcg ccggtaccgc ggccgcgtgg cgccgggaac tctgtcgccc
     1381 gcggaactgg ccgaggccgt ggcgcaactc gaggccatga acgaacggct ccgcaaaatc
     1441 gggacgtacg cgtcgttgct ctttgccgcg gacatgggcg tgccgcgcca cggcgccttg
     1501 taccaggcgt cccaggaaca gggcaccgcg ctgcggcagc gcctgacgtt tttcgatctg
     1561 gaatggagcg cggtgccgga ggctcgggcc aaggactacc tggcagctcc cgaattgtcc
     1621 cggtatcgcc attatctcga aatcgaacgg cgcctcgcgc cgtatcgact gtcggagccc
     1681 gaggaactgg tcctggagac caaggcgaac accggcgcgc acgcgttcca gcggttgttc
     1741 gatgaggtgt tggcgcgcgc gcgattcgac gtggtcgtcg gccgcaagcg ggaacggctg
     1801 agcgagcagg aaacgctggc gcgtctccac gcgcccgatc gcgcgacgcg caaagccgcg
     1861 gcccgggggc tgaccgcggg gc
//
LOCUS       DBNGTT010000155         1672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_671149, whole genome shotgun sequence.
ACCESSION   DBNGTT010000155 DBNGTT010000000
VERSION     DBNGTT010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_671149"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="AC16BC_03515"
     CDS             complement(<1..121)
                     /locus_tag="AC16BC_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR3240716.1"
                     /translation="MAKVVNAIVKLQIPAGKANPAPPVGPALGQHGVNIMEFCK"
     gene            complement(169..702)
                     /gene="nusG"
                     /locus_tag="AC16BC_03520"
     CDS             complement(169..702)
                     /gene="nusG"
                     /locus_tag="AC16BC_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3240717.1"
                     /translation="MGASWYVVHTYSGFENRVKQSIEERAKTYGLTEQIRKVMIPTEE
                     VVEIREGKRRMSTKKFFPGYVLVEMDMNDATWQLVKNTPKVTGFVGGGDTYPTPITED
                     ELSTLMSQMDSTAVKPRSKTQFQKGENVRIIDGPFLGFNGIVDEVHAEHSKVKVLVSI
                     FGRSTPVELGFLQVEKT"
     gene            complement(786..980)
                     /gene="secE"
                     /locus_tag="AC16BC_03525"
     CDS             complement(786..980)
                     /gene="secE"
                     /locus_tag="AC16BC_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR3240718.1"
                     /translation="MKNLYLRSKEFFAEVGSELKKVSFPTRTETMGSTSVVLIFVAAV
                     AIFLSAMDAVLVKLVAALLQ"
     gene            complement(1136..1212)
                     /locus_tag="AC16BC_03530"
     tRNA            complement(1136..1212)
                     /locus_tag="AC16BC_03530"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1176..1178),aa:Trp,seq:cca)
     gene            complement(1237..1386)
                     /gene="rpmG"
                     /locus_tag="AC16BC_03535"
     CDS             complement(1237..1386)
                     /gene="rpmG"
                     /locus_tag="AC16BC_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR3240719.1"
                     /translation="MREIITLACLDCKERNYSTMKNRRNTPDRLELSKYCGRCRKHTA
                     HKEVK"
     gene            complement(1536..>1672)
                     /locus_tag="AC16BC_03540"
     CDS             complement(1536..>1672)
                     /locus_tag="AC16BC_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR3240720.1"
                     /translation="VMPGDNVRMEVDLITPIAMEEGLRFAIREGGRTVGAGVITGIIA
                     "
BASE COUNT          303 a          582 c          434 g          353 t
ORIGIN      
        1 tcttgcagaa ctccatgata ttcacaccgt gctgacccaa cgccgggcca accggaggcg
       61 ccggattcgc cttcccggcc ggaatttgca gcttcacgat tgcgttcaca acctttgcca
      121 tagcgtccct ttcgcgtgtc tatcgaggtg cggccccttg atgcgcccct aggtcttttc
      181 gacctgcaag aaacccaact cgaccggggt ggagcgccca aagatgctca ccaacacctt
      241 gaccttcgaa tgttcggcgt gcacctcgtc gacgatgccg ttgaacccca ggaacggtcc
      301 gtcgatgatc cgcacgttct cacccttttg aaactgcgtc ttgctccgcg gtttgaccgc
      361 ggtggagtcc atctggctca tcaacgtgga cagttcgtcc tcggtgatcg gcgtggggta
      421 cgtgtcgccg ccgccgacga accccgtcac cttcggcgta ttcttcacga gctgccaggt
      481 ggcgtcgttc atgtccatct cgaccagcac gtatcccggg aaaaacttct tcgtggacat
      541 ccgccgcttg ccttcgcgaa tctcaacgac ctcctcggtc gggatcatga ctttgcgaat
      601 ctgctcagtc agtccgtacg tcttggcccg ctcttcgatg gactgcttca ctcggttctc
      661 aaaccccgag tacgtgtgca cgacgtacca cgacgctccc atgatgaccc ctcggctccc
      721 tgcctcttga tccgcggccc ccgtccggtc cgcggcgtcc gctccaacct gcccgcccgt
      781 catgctcatt gcagaagcgc ggccaccagt ttcaccagca ccgcgtccat cgccgacaag
      841 aagatggcga ccgcggcaac gaaaatcagc accacggacg tggaccccat ggtctcggtc
      901 cgagtgggaa acgacacttt cttcagttcg ctccccacct cggcaaagaa ctccttggac
      961 cgaaggtaca gattcttcat cgtctcatcc tcaatcgcct ttttcccgtg ggccaaccgc
     1021 ctgacctgaa aggggaagcc cctccctgta attccgtccg cccctcgtga caaattcccc
     1081 tgatcccaac cccgtccacc cgccgtggag caacggcgct ctccgcgagc gaggatggca
     1141 ggccaggagg gattcgaacc cccatcccgc ggttttggag accgctgctc tagccgttgg
     1201 agctactggc ctatcaaccg tcgccgacgg gatgtcctac ttgacttctt tatgcgccgt
     1261 gtgttttcgg caacgcccgc aatacttgct caactccagt cgatccgggg tgttccgccg
     1321 gttcttcatc gtggagtaat tgcgctcttt gcaatccaaa cacgccagcg taatgatctc
     1381 tcgcatagtt attccttttt acctttgccc aggagcctgg tcaggagcgc gccagatgca
     1441 aggcggagcg agacccggac cggagcgtac tgagtcgtac gcgaggaccg gaagcgcagc
     1501 gacaacgccg cagatggccg ctcatggaca ggctcctacg cgataatgcc ggtgatgacg
     1561 ccggcgccca cggtgcggcc gccctcgcgg atggcaaacc gcagcccctc ttccatcgcg
     1621 atcggcgtga tcaggtccac ttccatccgc acgttgtccc ccggcatcac ca
//
LOCUS       DBNGTT010000156         1874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1949673, whole genome shotgun sequence.
ACCESSION   DBNGTT010000156 DBNGTT010000000
VERSION     DBNGTT010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1949673"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..793)
                     /locus_tag="AC16BC_03545"
     CDS             complement(92..793)
                     /locus_tag="AC16BC_03545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3240721.1"
                     /translation="MNSLKFYVGKRLFRRAFRQVARPMLRGKVLDIGAGIAPYKNELA
                     HCRVVTLEVDPRLSPAVVGSAEALPFAAESFDAVICTEVLEHLREPGCALAEAFRILK
                     PGGGLYITTPMTWHLHYEPADYFRFTPHGVKYLLEKHRFRVTALAPLGGLSAVLSMVV
                     LEKVFNLCFKALFVVPKAHRARILAIVFVPCSWTVYQAAGLMDRWSKRWIFSVCAAAE
                     KPGSGASTDGGSAAQ"
     gene            complement(799..>1874)
                     /locus_tag="AC16BC_03550"
     CDS             complement(799..>1874)
                     /locus_tag="AC16BC_03550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3240722.1"
                     /translation="RGIDRKRFSPLVCCLGEPGAIGREIEQLGTEVIALHRMRHKRWD
                     WGIVRDLVELMKGRGVDIVHSHLYHANTYARIAAKIAGVKGVVATVHNVYAETKLKRR
                     WINYALAKGTDRLIAVSNAVRDDMVRYDWIDAHRIDVIHNGIDTAAFRPGGNRETVRR
                     DLDVPSDAFVIGCVSRLEPQKGHVHLLRAVQALASRHPSVHLVLVGDGSLREPLRNEA
                     DALGILSRTRLLGVRRDIPALFGAMDVFAMGSLWEGLPVAVLEAMASGLPVIATRVGG
                     LGEVIDDGRSGRLVPPADPQALAAAIESVLQHPEARRAMVAAGRRRVEEQFSTQAMVG
                     RLERLYDEIAARKGLRTSDGAAS"
BASE COUNT          339 a          669 c          567 g          299 t
ORIGIN      
        1 tggagcccct tcagggtctc cacgctcacc aagccgagtc cgctggcccc caacttggta
       61 aaacaggagt agatgacccg caccggcatc ctcactgggc cgcggatccg ccgtccgtcg
      121 acgcgcccga tcccggtttt tcagccgccg cgcagaccga gaagatccac cgtttcgacc
      181 accgatccat caatccggcc gcctggtaaa cggtccacga gcacggcacg aacacgatcg
      241 ccaagatccg ggcgcggtgg gctttgggaa cgacgaacag cgccttgaaa cacagattga
      301 acaccttttc caacaccacc atcgacagca ccgccgacag cccgccgagc ggcgcgagcg
      361 cggtgacgcg gaaccggtgt ttctcgagca aatatttcac gccatgcggc gtgaaccgga
      421 agtagtcggc gggctcgtaa tggaggtgcc aggtcatcgg cgtggtgata tagaggccgc
      481 cgcccggttt gagaatccga aaagcctcgg ccagcgcgca ccccggttcg cgcaggtgtt
      541 ccagcacctc ggtgcaaatc accgcgtcga agctctcggc cgcaaacggc agggcttccg
      601 cggaccccac gaccgcgggg gacaagcggg gatcgacttc cagcgtgacc acccggcagt
      661 gggccagttc gttcttatac ggcgcgatcc cggcgccgat gtccaacacc tttcctcgca
      721 gcatcggacg cgccacctgc cgaaacgccc gccggaagag acgcttcccc acgtagaatt
      781 tcagcgagtt cacgtccatc acgatgccgc cccgtccgac gttctgaggc cctttcgcgc
      841 ggcgatctcg tcatagagac gctccaatcg cccgaccatc gcttgcgtgc tgaactgctc
      901 ctcgacgcgg cgtcggccgg ccgcgaccat ggcccgacgg gcttcgggat gttgcagcac
      961 cgactcgatc gcggcggcca gcgcctgagg gtcggccgga ggcaccaggc ggcccgagcg
     1021 gccgtcatcg atcacttcgc ccagtccgcc gacgcgggtg gcgatcaccg gaaggcccga
     1081 ggccatggct tccaacaccg ccaccggcaa gccctcccac aaggagccca tggcgaacac
     1141 atccatggcg ccgaaaagcg cggggatatc gcgtctgacc ccgagcaatc tcgtgcgtga
     1201 caggatcccc agcgcgtcgg cttcgttccg caacggctcg cgaagcgacc cgtccccgac
     1261 gaggaccagg tgcaccgacg gatgacggct cgccagcgct tggaccgccc gcagcaaatg
     1321 cacgtggccc ttctgcggct ccaggcggct cacgcagccg atcacgaaag cgtccgaggg
     1381 cacgtccagg tctcgccgca ccgtctcccg atttcccccg gggcggaacg cggcggtgtc
     1441 gatcccgttg tggatgacgt cgatgcggtg cgcgtcgatc cagtcgtatc gcaccatgtc
     1501 gtcgcgcacc gcgttcgaca cggcgatgag ccggtcggtc cctttcgcca gcgcgtaatt
     1561 gatccatcgc cgcttgagct tggtttcggc gtagacgtta tgcacggtcg cgaccacgcc
     1621 cttgaccccg gcgattttcg ccgcgatgcg ggcgtaggta ttcgcgtgat acagatggga
     1681 gtggacgatg tcgacgccgc gccccttcat cagttcgacc aggtcgcgga caatccccca
     1741 atcccagcgt ttgtggcgca tccggtgcaa cgcgatcacc tcggtgccga gttgctcgat
     1801 ctctcgcccg atcgcccccg gctcgccgag gcaacacacc aacggggaga accgtttccg
     1861 gtcgatcccc cgca
//
LOCUS       DBNGTT010000157         2563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3354194, whole genome shotgun sequence.
ACCESSION   DBNGTT010000157 DBNGTT010000000
VERSION     DBNGTT010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3354194"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="AC16BC_03555"
     CDS             <1..419
                     /locus_tag="AC16BC_03555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3240723.1"
                     /translation="HYLEHLLDATRFYPGVEAVLAHFADKKKGVVTNKPIEYTIKLIE
                     GLGALERFDVILGGNSTPYLKPHPAMVVAALEAVGVAPDRAVMIGDNVNDIVAARAAG
                     VRSVAVGYGLGDPSILRAANPDFFCEQIQDVTELFS"
     gene            422..1120
                     /locus_tag="AC16BC_03560"
     CDS             422..1120
                     /locus_tag="AC16BC_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240724.1"
                     /translation="MPLIPRAVSVTLWLALILVAAGCATTRQSAVELNAGLLLVVPSS
                     TAQGWPRASDVAVRLERTPKVAVGWSRPVRSGDVEVRPTKAAREAWIAAVTRKLAESG
                     RVAGAVAIAPDRFDEDLTLAAVQHVAAEQGADVVLLFSFEVTKRRYHAFGAMADHADV
                     ESQVEVVTLAHAAGFTPSGVPVVTETQKGFASGTPERRSFEELQATSQRVAVNALSDA
                     IVARLRVIAPEAAR"
     gene            1117..>2563
                     /locus_tag="AC16BC_03565"
     CDS             1117..>2563
                     /locus_tag="AC16BC_03565"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3240725.1"
                     /translation="MTAPRSVFVLIIAGLLWVVPASAPAGSLLVDRVVDRTLDNGLRV
                     LIVKRSAVPTVSYNLTVRAGSVDEPGGESGLAHLFEHMAFKGTETIGTSDFSRERPLL
                     DELDRLEAEIQRAARSPGGGGSERLAELRRRFEETQAKARALVVPNEYGALYDRNGAV
                     GFNASTGADLTRYTVSLPANRLPLWIALEADRFSRPVFREFYTERDVVLEERRMRVEN
                     NPVGKLYEALFGAAFTAHPYRVPTIGWSSEVSALSAPDARAFFAGHYGPGNAVVAIVG
                     DVDVDDVLARVTASFGAIPARPRPMPIATREPSQSGERRIDVEADAQPETLVAYHRPG
                     IDDPDDPVFDVIESLLGSGRRSRLYTELVTTRQVAVAASASASEPGVRYPPVFVFELV
                     PRAPHTLDEVEAALSEQLERLQREPVSERELARVVNRLDADLIRSLQSNQGLASQLSY
                     FQAVAGDWRYVLTIRDRVAAVTPQDIQRVAST"
BASE COUNT          376 a          824 c          935 g          428 t
ORIGIN      
        1 gtcattacct ggagcatctg ctcgacgcga cgcggttcta ccccggggtg gaggcggtgc
       61 tcgcgcactt tgccgacaag aagaagggcg tcgtgaccaa caagccgatc gagtacacca
      121 tcaaactcat cgagggactg ggcgcgctcg agcgattcga cgtgatcctg ggcgggaaca
      181 gcacgccgta cctcaagccg catcccgcga tggtggtcgc ggcgctcgag gcggtgggcg
      241 tcgcgccgga tcgggcggtg atgatcggcg acaacgtcaa cgacatcgtg gccgcgcgcg
      301 cggccggggt ccggtccgtg gccgtggggt acgggttggg tgacccctcg atcctgcgcg
      361 cggcgaatcc cgactttttc tgcgaacaga tccaggacgt gaccgagctc ttctcctgac
      421 gatgcccctg atcccgcgcg ccgtatccgt cacgttgtgg ctggcactga tcctggtcgc
      481 cgcggggtgc gcgaccacgc gccagtccgc cgtggaattg aacgcgggcc ttctgctcgt
      541 ggtcccgtcg tccacggctc aagggtggcc gcgagcgtcc gacgtcgcgg ttcggctcga
      601 acggacacca aaagtggccg tgggctggtc tcgtccggta cggtcgggcg acgtcgaggt
      661 gcggcccacg aaagccgcgc gcgaggcctg gatcgcggcc gtcacgcgca aattggcgga
      721 gagcgggcga gtggccggcg cggtggcgat cgccccggat cggttcgacg aagacctgac
      781 gctcgccgcg gtgcaacacg tggccgccga gcagggcgcg gacgtggtcc tgctgttttc
      841 tttcgaggtg acgaaacgac ggtaccacgc gttcggggcc atggcggatc acgccgatgt
      901 ggagagccaa gtggaggtcg tgaccttggc tcacgcggct ggtttcaccc cgtccggggt
      961 ccccgtggtg accgagaccc agaagggctt tgcgtccggc accccggaac gacgcagttt
     1021 cgaggaactc caggcgacgt cccagcgtgt cgcggtgaac gcgttgtccg acgcgatcgt
     1081 cgcgcggttg cgggtgatcg ctccggaggc cgcgcgatga cggcgccccg ctccgtcttc
     1141 gtcctgatca tcgcgggtct gctgtgggtc gtccccgcgt cggcgccggc cggctcgctg
     1201 ctggttgatc gcgtggtgga ccggacgctg gacaatgggc tgcgggtgct gatcgtgaaa
     1261 cgctccgcgg tgcccaccgt gtcgtacaac ctcaccgtgc gcgcggggtc ggtggatgaa
     1321 ccgggcggcg agagcgggtt ggcccacctg ttcgaacaca tggcgttcaa gggaaccgag
     1381 acgatcggca ccagcgactt ttcgcgcgaa cgaccgttgc tggatgaatt ggatcggctc
     1441 gaagccgaga tccaacgcgc ggcgcgttcg ccgggaggcg ggggctccga gcggctggcg
     1501 gagttgcgac ggcggttcga ggagacgcag gcgaaggcgc gcgccttggt ggtgcccaac
     1561 gaatacggcg cgttgtacga ccgcaacggc gcggtcgggt tcaacgccag cacgggcgcg
     1621 gatctgaccc gctacacggt gagcctcccg gccaaccggc tcccgttgtg gatcgcgttg
     1681 gaggccgacc ggttcagccg gcccgtgttc cgcgagtttt acaccgagcg cgacgtggtc
     1741 ctggaggagc ggcggatgcg ggtggagaac aacccggtcg gcaagctcta cgaggccctg
     1801 ttcggcgcgg cctttaccgc gcatccctat cgcgtgccca ccatcggctg gagttcggag
     1861 gtgtccgcgc tctccgcgcc ggacgcccgg gcctttttcg ccggccacta cggaccgggc
     1921 aacgcggtgg tcgcgatcgt cggcgacgtg gacgtggacg acgtgctcgc gcgggtgacg
     1981 gcgtccttcg gcgcgattcc cgcgcgtccg cggcccatgc cgatcgcgac gcgggaaccc
     2041 tcgcaatccg gcgagcggcg gatcgacgtc gaggccgacg cccagccgga gacgctcgtg
     2101 gcctatcacc gccccgggat cgatgatccc gatgatccgg tgttcgacgt gatcgaatcg
     2161 ttgctcgggt ccgggcgacg gtcgcgcctc tacacggagc tggtgaccac gcgtcaagtc
     2221 gccgtggccg cctcggcctc cgcgagcgag ccgggagtcc ggtatccgcc ggtgttcgtg
     2281 ttcgagctgg ttccccgggc gccgcacacg ctcgacgaag tcgaagccgc gttgtcggag
     2341 caactcgaac gtctgcagcg cgaaccggtg tccgaacgcg aactggcccg ggtggtgaac
     2401 cggttggacg cggacctgat tcgttcattg cagtccaacc agggcttggc gtcgcagttg
     2461 agctactttc aagccgtggc cggggactgg cggtacgtgc tgaccatccg ggatcgcgtg
     2521 gccgcggtga cgccgcagga tatccagcgg gtggcgtcga cgt
//
LOCUS       DBNGTT010000158         6183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1182314, whole genome shotgun sequence.
ACCESSION   DBNGTT010000158 DBNGTT010000000
VERSION     DBNGTT010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1182314"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="AC16BC_03570"
     CDS             <1..1645
                     /locus_tag="AC16BC_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3240726.1"
                     /translation="QELATSTSANIDTLFSHHSEEARLLASIQSVLTVVEESNAFFGS
                     DPPETVHARIRDIDERWRTAKGVDAFFLEIQGNRATASLKEFVAQTPNPELYQAILVT
                     NLYGALVAAAGNTTRYDYGQTSWWTHAYHEGAGRLFISGGETPPGGDAAVIVIATPII
                     KNGRAIGVLALIQRADPLLRGAASAKIGKTDNTMILAKDGTVLFCPIAPAQSHSLTPA
                     HIAAVTQDQAGWVATRDDVHFPGREALNGHAPVAITQRLGADNFGGQPWYIVTSQDPA
                     ESYASIYTLLRWIGLAGVTGTAIFVLLGLLIARRIVQPIQTLQAGAELIGQGNLNHRI
                     EISTGDEIEDLANQFNKMAHKLKLFYIGLEENVKEKSWKIEHQNKELSILYAIAATLN
                     QALPIRDLLDQTLNKMLDVMEADGGLIWMAQPPSGSSPITATKLPTLSPAQMSSLIEL
                     IHHISLQIRRNGELWATENLAVDDRIESVRSSDPGFISLVGIPLNAHDHVLGILFLLY
                     RDIRALTSREEKLLTSVGSQIGITIEHSAAAPSHGPLTP"
     gene            1701..2303
                     /locus_tag="AC16BC_03575"
     CDS             1701..2303
                     /locus_tag="AC16BC_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3240727.1"
                     /translation="MTIFRVGGQERSRKTEFEDQTLPYLDSLYGFAVVLTGNSDDAHD
                     LVQDAYLRAFRFYPRYEHIANYKAWLFTLTKNLFINRYHQRAREISLADLDIVDDDRY
                     ESAAAIVLSARDRHERGLFRQDFESALAAMPEKLRVAVLLKDVEGFDYKEIAEIMGCP
                     LGTVMSRLSRGRNLLKRTLSDYWNARESGRLDAGAHTHSS"
     gene            2305..3123
                     /locus_tag="AC16BC_03580"
     CDS             2305..3123
                     /locus_tag="AC16BC_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3240728.1"
                     /translation="MDPCDSIRKLMTPYLDGELDVKESLRVDAHLQECEDCRETFLAD
                     KAFLTIVHDAAVVAPAPEFTARCLRAALDREVRQSPPRRRSRLPWIAAVVGLATAATL
                     LLNLGGSRHPVPDLVQLAVNEHRAFLRAPDGLDIRSDDGALVSSWLQSRLHFDIDIPK
                     TTPPGYHLEGARIAADREPRAAYVAYTVGDETVSLLVTPPQEIRFAGREVISFRNMLF
                     HPADVDGYHTLEWSDSRHTYVLVASSPRLVSDACQLCHSSNRDRRVIDGFANGV"
     gene            complement(3292..4554)
                     /gene="clpX"
                     /locus_tag="AC16BC_03585"
     CDS             complement(3292..4554)
                     /gene="clpX"
                     /locus_tag="AC16BC_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR3240729.1"
                     /translation="MDDKRKSDRIETLRCSFCGKSRDEVRKLIAGPTVYICDECIELC
                     NDIIAEESEGQKQVAASATLLKPIEIKRILDQYVVGQERAKKVLAVAVHNHYKRVNAG
                     SSTSDVELQKGNILLIGPTGTGKTLLAQTLARILDVPFTIADATTLTEAGYVGEDVEN
                     IILKLLQAADYDVEKADRGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGT
                     VANVPPQGGRKHPHQEFIQVDTSQILFICGGAFVGIESLVGQRLQTRVMGFGADIPSR
                     EDRRVGELLSQVEPEDLLKFGLIPEFVGRLPVHATLDDLDEQALIKILSEPKNALVKQ
                     YERLFAFEKVRLKFTAGALAEVARRAHAQRTGARGLRAILERVMLELMYEIPSAKNVK
                     ECIITEEAIQNTAQPILVYDQDMGVKSA"
     gene            complement(4602..5198)
                     /gene="clpP"
                     /locus_tag="AC16BC_03590"
     CDS             complement(4602..5198)
                     /gene="clpP"
                     /locus_tag="AC16BC_03590"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR3240730.1"
                     /translation="MLVPMVIEQTNRGERAYDIYSRLLKDRIILVGTPIDDTSANLII
                     AQLLFLEAEDPAKDISMYVNSPGGLVTAGLAIYDTMQYIKPDVSTICIGQASSMGAVL
                     LAAGARGKRFALPNARIMIHQPLGGFQGQATDIGIHAKEILRTRERLNQILAKHTGQT
                     LERIQTDTERDYFLSGEESKDYGLIDNVLERGPAVPKT"
     gene            complement(5242..>6183)
                     /gene="tig"
                     /locus_tag="AC16BC_03595"
     CDS             complement(5242..>6183)
                     /gene="tig"
                     /locus_tag="AC16BC_03595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3240731.1"
                     /translation="LTRRRVQVTDADLAEALESVRLRQAELVSYDPEHVIADGDFAVI
                     DFEGVSDGQPVPGAGATGYLVHVGAQSVIPEIEAALIGKRSGDRVEAEVTFPADYQNA
                     RVAGRRVLFRISIGDVKQRVLPALDDELAKDLGLASIGELRERIRTELEARQRAEHRA
                     EERSAVLKQLSDRHQVDLPPSLVQREAELLRDRVERHLGGAVPGQGAPGGDAETFKRE
                     VVGAAEERVKGDLLLEAIAEREGLSVGADEIEREVQRIAAQTRSDVREVRHLLAGESG
                     EFVGLTATIRREKTLDWLIDHAEIREEDGSTGGQEPR"
BASE COUNT         1135 a         2078 c         1751 g         1219 t
ORIGIN      
        1 ccaggagctc gcgacttcga cgagcgccaa catcgacacg ctgttctccc accatagcga
       61 ggaagcgcga ctgctggcgt cgatccagtc cgtcttgacc gtggtggagg agtcgaacgc
      121 gttcttcggc agcgatccgc ccgaaacggt gcacgcccgg atccgcgaca tcgacgagcg
      181 ttggcgtacc gcgaagggcg tggacgcctt cttcctcgaa attcagggca accgggcaac
      241 cgcctcgctc aaggaatttg tcgcgcagac gccgaatcct gagctctacc aagccattct
      301 cgtcaccaat ctctacggcg cgctcgtcgc cgccgccgga aacacgaccc gttacgacta
      361 cggccagacg tcgtggtgga ctcacgcgta ccacgaggga gcggggcgcc tgttcatcag
      421 cggcggcgaa accccgccgg gcggagatgc cgccgtcatc gtgatcgcga ccccgatcat
      481 caagaatggc cgcgcgatcg gcgtgttggc gctcattcaa cgcgccgacc cgctgctgcg
      541 cggagccgcc tcggccaaga tcggaaagac cgacaacacc atgattctcg ccaaggacgg
      601 gaccgttctc ttctgcccga tcgcgccggc ccaaagccat tcgttgacgc ccgcgcacat
      661 cgccgcggtc acccaggatc aggccggatg ggtcgcgacc cgcgacgacg tccactttcc
      721 cgggcgcgaa gccctcaacg gccacgcccc ggtcgcgatc acgcagcgtc tgggcgccga
      781 caacttcggc ggccagccct ggtacatcgt gaccagtcag gaccccgcgg aaagttacgc
      841 ctccatctac acgctgctcc gctggatcgg tttggccggc gtgaccggaa ccgcgatctt
      901 cgtccttctc ggcctgctga tcgcgcgccg catcgtgcaa ccgattcaaa ccttgcaggc
      961 cggcgcggag ttgatcgggc agggaaacct caatcaccgg atcgagatct ccaccggcga
     1021 cgaaatcgag gacctcgcaa accaattcaa caaaatggcc cacaagctca aactgtttta
     1081 catcgggctc gaagaaaacg tgaaagaaaa gagctggaag atcgagcatc agaataaaga
     1141 gctctcgatc ttgtacgcca tcgccgccac cttgaaccaa gccctcccca ttcgggacct
     1201 gcttgatcag accttgaaca agatgctcga cgtgatggag gccgacgggg gcctcatctg
     1261 gatggcgcag ccgccgtcag gatcatcgcc catcacggcc accaaactgc cgactctttc
     1321 gccggcgcaa atgagcagcc tgatcgaatt gatccaccac attagcctgc aaatccgacg
     1381 caacggggag ctctgggcca ccgagaacct cgccgtggat gaccgcatcg aatcggtgcg
     1441 gtccagcgat ccgggcttca tttctttggt cggaattccc ttgaacgccc atgatcacgt
     1501 cctcggcatc ctgttcttgt tgtaccgcga tatccgggcg ctcacgtccc gggaggaaaa
     1561 gcttctgacg tccgtgggca gtcaaatcgg cataactatt gaacatagtg cggcggcgcc
     1621 cagtcatggg ccgctgacgc cgtagccgaa aagacgcgct cgcggaagat tttggcggcc
     1681 tgggaggtca tttctgtaca atgacgatct ttcgagttgg cggccaagag cggtctcgga
     1741 agaccgaatt tgaagaccag acgcttccct atctggactc gttgtacggt tttgccgtgg
     1801 tcctgaccgg caacagcgac gacgcgcacg acctggtcca ggatgcctac ttacgggcgt
     1861 ttcgcttcta tccccggtat gagcacatcg ccaactacaa ggcatggctg tttaccctca
     1921 cgaaaaacct ctttatcaac cggtatcatc agcgggcgcg ggaaatctcc cttgcggatc
     1981 tcgacattgt tgacgacgac cgctatgagt cggccgccgc gattgtcctc tccgcgcgcg
     2041 accgccatga gcggggactc tttcgccagg atttcgagtc cgcgctggcc gccatgccgg
     2101 agaagctccg ggtggcggtc ctcttgaaag atgtcgaggg ctttgactat aaagaaatcg
     2161 cggagatcat gggctgtccc ttggggaccg ttatgtcgcg cctctcgcgc ggacgaaacc
     2221 tgctgaagcg gacgttgagc gactactgga atgcccgcga gtcgggtcgc ctggacgccg
     2281 gcgcccacac ccactcgagt tgacatggac ccctgcgact cgatcaggaa gttgatgacg
     2341 ccgtacctgg acggggagct ggacgtgaag gaatccctgc gcgtcgacgc gcatctccaa
     2401 gaatgcgagg attgccggga aaccttcctc gcggacaaag cgttcctgac catcgtccat
     2461 gacgccgccg tggtggcccc cgcgcccgag tttaccgcgc gctgcttgcg agccgccctc
     2521 gatcgcgagg tccgtcaatc gccgcctcgg cgccgatccc ggctaccatg gatcgcggcc
     2581 gtcgtcggtc tcgcgaccgc cgcaactctc ttgctcaacc tcggagggtc gcgtcaccca
     2641 gtgccggatt tggtgcaact cgccgtaaac gagcaccgag catttttacg cgccccggac
     2701 ggtctcgaca tacgaagtga tgacggagcc cttgtgtcgt catggctgca atcccgtctt
     2761 catttcgata tcgacatccc gaagaccaca ccgccgggtt atcatctgga gggtgctcgg
     2821 atcgccgccg atcgcgaacc ccgagccgcc tacgtggcct acacggtggg agacgagacc
     2881 gtctcgttgt tggtcacccc gccgcaggaa atccgcttcg cgggccgtga ggtgatcagt
     2941 ttccgaaaca tgctctttca ccccgcggac gtcgacggct accacacgct ggagtggtcg
     3001 gacagccgac acacctacgt cctggtggct tccagcccgc gcctggtttc ggacgcctgc
     3061 caactctgcc acagctcaaa ccgagaccgt cgggtcatcg acggattcgc caacggcgtc
     3121 taacgatcga ggggagcggg aaacgcgcgc ccgcacccgc caccatcgca accctcttca
     3181 cggcttgata accgacggga ccggactgac gcgtcggtgc gacgtcgcac ggcggaagcc
     3241 ccctgtcacg aagtccgtac tgacgaagga aggcgagcgc ccgccattgg gctaggcgga
     3301 cttcacgccc atgtcctggt cgtagaccag aatgggctgc gcggtgtttt gaatggcttc
     3361 ttcggtgatg atgcactctt tgacgttctt tgccgaagga atctcgtaca tcaattccag
     3421 catcacccgt tccagaatcg cgcggagacc gcgcgccccg gttcgctggg cgtgcgcgcg
     3481 ccgcgccacc tccgccaacg cgccggccgt aaatttcagg cgcaccttct cgaaggcgaa
     3541 cagtcgctcg tattgtttca ccagcgcgtt cttcggttcg ctcagaatct tgatcagcgc
     3601 ttgttcgtcc agatcgtcga gcgtcgcgtg cacgggaagg cggccgacaa attccgggat
     3661 caacccgaat ttcagcaagt cctcgggctc gacctgcgac agcaactcgc cgacgcgtcg
     3721 atcctcacgg ctcggaatgt ccgcgccgaa tcccatcacg cgggtctgca gccgttggcc
     3781 gacgagcgac tcgattccca caaacgcgcc gccgcagata aacaagatct ggctcgtgtc
     3841 gacctgaatg aattcctgat gcgggtgttt ccgcccgccc tgcggaggaa cattcgccac
     3901 ggttccttcg atgagcttca gcaacgcttg ctgtacgccc tcccccgaaa cgtcccgggt
     3961 gatcgacggg gaatcgcttt tgcggctgat cttgtcgatc tcgtcgatgt agacgatgcc
     4021 acggtccgcc ttctccacat cgtagtccgc cgcctgaagg agcttgagga tgatgttctc
     4081 gacgtcttcg cccacatacc cggcctcggt caaggtggtg gcgtccgcga tcgtaaaggg
     4141 aacgtcgagt atccgggcca gcgtttgcgc caacagcgtc ttgccggtcc ccgtcggtcc
     4201 gatcagcagg atattgccct tctggagttc gacgtcgctc gtgctggacc ccgcgttcac
     4261 gcgtttatag tggttgtgaa ccgcgaccgc gagcactttc ttggcgcgct cctgacccac
     4321 gacatattga tcaaggattc gtttgatctc gatcggtttg agcagcgtgg ccgacgcggc
     4381 cacttgcttc tggccctcgg actcctcggc gatgatgtcg ttgcagagtt cgatacactc
     4441 gtcgcagata tagacggtcg gccccgcgat gagtttgcgg acttcgtccc ggcttttccc
     4501 gcagaatgaa cagcgcagcg tttcgatccg gtccgatttc cgtttgtcgt ccaccgtgtc
     4561 tccctctctc gcgcggcccg cggccttttc gagccctctt gttacgtctt cggtacggcc
     4621 ggaccccgtt caagaacgtt gtcgatcagg ccatagtcct tggactcttc cccggagagg
     4681 aagtagtccc gttccgtatc cgtttgaatc cgctccagcg tctgcccggt gtgtttggcc
     4741 aggatctgat tcaaccgctc gcgcgtccga agaatctctt tggcgtgaat gccgatatcg
     4801 gtcgcctgac cttggaatcc gcccaacggt tgatgaatca tgatccgggc gttcgggagc
     4861 gcgaaccgct ttccgcgcgc tccggccgcc aacaacacgg cccccatgct cgaggcctga
     4921 ccgatacaaa tcgtcgacac gtcgggcttg atgtactgca tcgtgtcgta aatcgcgagc
     4981 cccgcggtca ccagcccccc cgggctattc acgtacatgc tgatgtcctt ggcgggatcc
     5041 tccgcctcga gaaataacaa ctgcgcaata atcaaattcg ccgaggtgtc gtcgatcggg
     5101 gtgcccacca gaatgatccg atctttcagg agacgcgaat agatatcgta ggcccgctct
     5161 ccacggttcg tttgttcaat caccatcggt accaacatgc tcgctcctta cgaaaacgaa
     5221 aggggttccg tccccccgtg tttatctagg ctcctgaccg ccggtggagc cgtcttcttc
     5281 cctaatctct gcgtggtcga tcaaccaatc caaggttttt tctcggcgga tcgtggccgt
     5341 gaggcccacg aactcgccgc tctccccggc aagcagatgg cgcacttccc ggacgtccga
     5401 ccgcgtttgc gccgcgatcc gctgaacctc gcgttcaatt tcgtccgctc caaccgacaa
     5461 cccttcccgc tccgcgatcg cttccaacag caagtcgccc ttcacccgtt cctcggccgc
     5521 gcccacaacc tctcgcttga acgtctccgc gtcccccccg ggcgctccct gtcccggcac
     5581 ggcgcctccc agatggcgtt ctacgcggtc ccgcagcaat tccgcctccc gttggacaag
     5641 gctcggcggc aaatccacct gatggcgatc gctcaactgt ttcaataccg cgctacgctc
     5701 ttccgcccgg tgttcggccc gctggcgagc ttcgagttcc gtccggatac gttcgcgcaa
     5761 ctctccgatc gacgcgaggc cgagatcctt ggccaattca tcgtccaacg ccggaagcac
     5821 gcgctgcttc acgtctccga tgctgatgcg aaagaggacc cgtcggcccg cgacccgtgc
     5881 gttctgatag tccgccggga acgtcacctc ggcctcgacg cggtccccgc tgcgcttacc
     5941 gatgagggcc gcctcgatct ccggtatcac ggattgggct ccgacgtgca cgagataccc
     6001 ggtggccccg gcccccggca ccggctgacc gtccgacacg ccttcgaagt cgatcaccgc
     6061 gaagtcgccg tccgcgatca cgtgctccgg gtcataactc accaattccg cttggcgcag
     6121 ccgcacgctt tccagggctt cagccagatc ggcgtccgtg acctggacgc gtcggcgagt
     6181 caa
//
LOCUS       DBNGTT010000159         1620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_672261, whole genome shotgun sequence.
ACCESSION   DBNGTT010000159 DBNGTT010000000
VERSION     DBNGTT010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_672261"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1291)
                     /locus_tag="AC16BC_03600"
     CDS             complement(143..1291)
                     /locus_tag="AC16BC_03600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3240732.1"
                     /translation="MKIALIRKSYTPFGGAERHVDRFLDALRERGHDVHLFAARWNGE
                     TRRGLALHRVPVIGATRWAEAWSFAVRASRLAAAGGFDLVHSFDRVWSCDVYRAGDGV
                     HREWLQRRRAAQPAWRRAIPSVNPLHRVYLALDRGLFEGRCKVVIANSARGKAEILAH
                     YRADPDRIRVVYNGVDLERFRVPSPNERDAARQAASLAPSERLAILVGSGFERKGAAA
                     AVRAVASLAKTRAAVTRLAVIGRGRTEPYQRLARALGVADRVSFVGPVVDVRPWYAAA
                     DALILPTIYDPFANVCLEALASGVPVVTSAANGAAEILNKDVGAVIEDASDAQAFAGA
                     LDRLWTDARNRAPACRAVAERFSEDRQVAETLGVYDELLSGAKTSAAR"
     gene            complement(1370..>1620)
                     /locus_tag="AC16BC_03605"
     CDS             complement(1370..>1620)
                     /locus_tag="AC16BC_03605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3240733.1"
                     /translation="VGAVSEVVQDARTGVLARARDPKSLADGVVRLLTQAELRRSVAA
                     AGRALVEKNYTLAAMLDHTEALYQRCLSGPPSRAAARV"
BASE COUNT          270 a          601 c          515 g          234 t
ORIGIN      
        1 acaataagtg aatagtcatg catgccccca gcctgtgcat cctgcattgc ggggcagccc
       61 cgtggcgcta acctgcttga tgtcaaatac aagacgtgac cccggcgact agaaagcaca
      121 tggcatgact ttagaggccg gtctaccgcg ctgcgctggt cttggctcca ctcagcaatt
      181 cgtcgtacac gcccaaggtc tccgccacct gccgatcctc tgaaaagcgt tccgccacgg
      241 ctcgacaggc gggcgcgcgg tttcgcgcat cggtccagag ccgatcaagt gcgccggcga
      301 acgcctgggc gtccgacgca tcctcgatca ccgcgcccac gtccttgttg agaatttccg
      361 cggcgccgtt ggccgcgctg gtgaccaccg gcaccccgct ggccagggct tccagacaca
      421 cgttggcaaa gggatcgtag atcgtgggca ggatcaacgc gtccgcggcc gcgtaccacg
      481 gccgcacgtc aacgaccggc cccacgaacg acacgcgatc cgccaccccg agcgcgcgcg
      541 ccaggcgctg atacggctcg gtccgcccgc ggccgatcac ggccagccgg gtcacggcag
      601 cgcgggtctt ggcgagcgag gccacggcgc gcaccgcggc ggccgcgcct tttcgctcga
      661 atcccgaacc caccaggatc gcaaggcgct cactcggggc caggcttgcg gcttgtctgg
      721 ccgcgtcgcg ctcgttcggg cttggcacgc ggaaccgctc caggtccacg ccgttgtaca
      781 cgacccgaat ccggtctgga tcagcccggt agtgcgcgag gatctcggcc ttgccgcgcg
      841 ccgaattcgc gatcacgacc ttgcaacgcc cttcgaacaa tccgcgatcg agcgccagat
      901 acacgcggtg caagggattc accgacggga tcgcgcgccg ccacgcgggt tgcgcggcgc
      961 ggcgacgctg caaccactcg cggtgcacgc cgtcgcccgc gcggtacacg tcgcacgacc
     1021 acacccggtc gaagctgtgc accaggtcga atccgcccgc ggccgccagg cgcgaggcgc
     1081 gtaccgcgaa gctccaggcc tcggcccagc gcgtcgcccc gatgaccggc acccggtgca
     1141 aggcgaggcc gcgacgcgtc tccccgttcc agcgcgccgc gaacaggtgc acgtcgtgtc
     1201 cccgctcgcg cagcgcatcg aggaaccgat ccacgtgccg ttccgcaccg ccgaacggcg
     1261 tatacgattt acggatgagg gcgatcttca cggaatttgg gacgtgtcgg tcatccggca
     1321 atcgcgcgaa cgcttcgcga ccggacgctc gtcgcggacg ccgcggctct cagacacggg
     1381 ctgcggcgcg ggacggcggg ccggacagac accgttggta gagcgcctcg gtgtgatcca
     1441 acatggcggc cagcgtatag tttttctcga ccagcgcgcg tcccgcggcc gccacggatc
     1501 gccgtaactc cgcctgcgtc aacaggcgca ccaccccgtc cgccagcgac ttcggatcgc
     1561 gcgcgcgcgc cagcacgccg gtccgagcgt cctgaaccac ctcggacacg gcgccgacat
//
LOCUS       DBNGTT010000160         2070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3227267, whole genome shotgun sequence.
ACCESSION   DBNGTT010000160 DBNGTT010000000
VERSION     DBNGTT010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3227267"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="AC16BC_03610"
     CDS             <1..227
                     /locus_tag="AC16BC_03610"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240734.1"
                     /translation="FRAAIQVNPGFAMGFFNLGRALQETGARDEAIAAFQRFLALAPS
                     QPGAMPSIPQARQRLGELESGVPAQSPPPN"
     gene            284..1486
                     /locus_tag="AC16BC_03615"
     CDS             284..1486
                     /locus_tag="AC16BC_03615"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR3240735.1"
                     /translation="MEFDTIEAAVADIRAGRMVILIDDEDRENEGDLVLAADKVTPEA
                     INFMARHARGLICVAITPERAEALQLTPMASENTATFGTAFTVSVDARVGVTTGISAS
                     DRAATIQAIIDPSTKPADLARPGHVFPIRAQPGGVLKRAGQTEGSVDLATLAGLEPAG
                     VICEIMNEDGTMARVPQLREFAVRHGLKIVTIKHLIEYRMKRESFVRRLAEAELPTRY
                     GEFRVVVYENTIDHGTHVALIKGEVGGSDGTLVRVHSGCVTGDVFSSMRCDCGEQLHA
                     AMAQIQKEGRGVLLYLNQEGRGIGLVNKIKAYALQDQGHDTVEANLALGFAPDLRDYG
                     IGAQILVNLGIRRIRLMTNNPRTVVGLDGYGLTVTERVPLETLPHESNREYLRTKKHK
                     LGHMLNEV"
     gene            1528..1992
                     /gene="ribH"
                     /locus_tag="AC16BC_03620"
     CDS             1528..1992
                     /gene="ribH"
                     /locus_tag="AC16BC_03620"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3240736.1"
                     /translation="MARAAANRGLRVAIAASRFNETITARLVSAARAELTAGGVDRRA
                     VTVVYVPGAFELPLAAQWLCRAGRYDAVICLGTVIRGDTPHFDFIARAASQGIMDVGL
                     AEGRPVVFGVLTTETVEQAEERADASRLNRGAEAARTAIEMARLAKRFRREG"
BASE COUNT          378 a          605 c          729 g          358 t
ORIGIN      
        1 agtttcgtgc cgcgatccag gtcaaccccg gttttgcgat gggttttttc aacctgggga
       61 gggcccttca ggaaacgggc gcgcgggacg aggcgatcgc ggcgtttcaa aggtttttgg
      121 ccctggcgcc gagtcagccg ggtgcgatgc cgagcattcc tcaggcccgt cagcggttgg
      181 gcgaactcga aagcggggtg cccgcgcagt cgccgcctcc gaattgacaa agtccacagg
      241 ctttcttatg atagcaatct cggtaaaact ccaagaaaaa tcaatggaat ttgacacaat
      301 cgaagccgct gtcgcggaca tccgggcggg gcgcatggtc atcctcatcg acgatgagga
      361 tcgggaaaac gaaggcgacc tggtgttggc cgccgataaa gtcacgcccg aggccatcaa
      421 cttcatggcg cgccacgcgc gggggctcat ctgcgtggcc atcacgccgg aacgcgcgga
      481 ggcgctgcag ttgacgccga tggcgtcgga gaacacggct acctttggca cggccttcac
      541 cgtctccgtg gatgcgcgcg tcggcgtgac caccgggatc tcggcgagcg atcgcgccgc
      601 gacgattcag gcgatcattg atccgtccac caaacccgcg gacttggccc gcccgggtca
      661 cgtgttcccg atccgtgcgc aacccggcgg ggtgctcaaa cgcgccgggc aaaccgaagg
      721 gtcggtggac ctcgccacgt tggccggatt ggagccggcc ggcgtaattt gcgagatcat
      781 gaacgaagac gggaccatgg cgcgcgtgcc gcagctcaga gagtttgcgg tccggcacgg
      841 gctgaagatc gtgacgatca aacatttgat cgaataccgc atgaagcgcg agtcgtttgt
      901 gcggcggctg gccgaggccg aattaccgac ccggtacgga gagttccggg tggtggtcta
      961 cgaaaacacc atcgaccacg gcacccacgt ggccctgatc aagggggagg tgggcggatc
     1021 agacggcacg ctggttcgcg tgcattcggg ctgcgtgacc ggcgacgtgt tcagttccat
     1081 gcggtgcgac tgcggcgagc aactccacgc cgccatggcg cagatccaga aggagggccg
     1141 cggcgtcctg ttgtatctca atcaggaagg gcgcggcatc gggttggtca acaagatcaa
     1201 ggcctacgcg ctccaggacc aagggcacga taccgtggaa gccaacctgg cgttggggtt
     1261 cgcgcctgat ttgcgcgact acgggatcgg agcccaaatc ctggtcaacc tcggcattcg
     1321 acggattcgg ctcatgacca acaacccgcg gacggtggtt ggcctggacg gctacgggtt
     1381 gacggtgact gagcgtgtcc cgttggaaac gctcccccac gagagcaatc gcgagtacct
     1441 ccggacgaag aagcacaaac tcggtcacat gttgaacgag gtatgaccgc ccgccacgcg
     1501 gtcaaagggc ccgggcgctc gcgcgccatg gctcgtgcgg cggcgaaccg tggtcttcgc
     1561 gtggcgatcg cggcgagccg gttcaatgag accatcacgg cgcgactggt gagcgccgcg
     1621 cgcgccgagc tgacagcggg cggggtggat cggcgggctg tgaccgtggt ctacgtgccg
     1681 ggggcgttcg agttgccgtt ggcggcgcag tggctctgcc gcgcggggcg atatgacgcg
     1741 gtgatttgtc tgggaacggt gatccgcggc gatacgcctc attttgattt catcgcgcgg
     1801 gccgcgtcgc aggggatcat ggacgttggg ctggccgagg ggcgtcccgt ggtgttcggc
     1861 gtcttgacca cggagaccgt ggagcaggcg gaagagcggg ccgatgcctc gcggctcaat
     1921 cgcggagccg aggcggctcg aaccgcaatc gagatggcgc gattggccaa acgcttcagg
     1981 cgcgaagggt gatgcaacgt gggctttcgt cgtaaagggc gcgagtacgc gcttcaagtg
     2041 cttttcgggt cggatctggc ctgcgccgag
//
LOCUS       DBNGTT010000161         7297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_288325, whole genome shotgun sequence.
ACCESSION   DBNGTT010000161 DBNGTT010000000
VERSION     DBNGTT010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_288325"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="AC16BC_03625"
     CDS             <1..854
                     /locus_tag="AC16BC_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HflX-like GTP-binding protein"
                     /protein_id="HIR3240737.1"
                     /translation="AAFDAIEAWLRVTGRSVVYRVWQRRRAPDTATYIGRGKAVQLRE
                     LCERAGVDGVLINGTLSRSQRDGLLRLLDRPVWDRTVWGATQKPPVPVSRARELARAA
                     RRRNGTFTVVLCGAAGAGKSTLFAALTRGAVSPPSSWPVGPRAAGPGVVSRRLRFAPG
                     TGDVVVTDTPGLIRNPDSAAWIVPDETLDECRGADLVVHVIDASHPEANRRAREVDAA
                     LVRAGIHPALSVRTQADRISTGSDGIAPGLASGRTGCGCDALRALVRRMAAHRAPRLV
                     RPRGFML"
     gene            887..1459
                     /gene="lptC"
                     /locus_tag="AC16BC_03630"
     CDS             887..1459
                     /gene="lptC"
                     /locus_tag="AC16BC_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR3240738.1"
                     /translation="MAARWTAWRALPLWLLIIGGLVALGVVLVSHVRVRGTAVPLSPS
                     RVDGDLILTGFSLSTLLNGEREWEVSAARARLFEPDHHAVLDEVRGTVRLRNGSLMQF
                     EGVSAIFDTVSHDLDLEGQDLGAVVTLPNGFVLRANRLRWTQEQGELSSDDSIVLVGP
                     RLSIRGVGLRAWPSSQEFTILNSVRADVSS"
     gene            1446..2039
                     /locus_tag="AC16BC_03635"
     CDS             1446..2039
                     /locus_tag="AC16BC_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR3240739.1"
                     /translation="MCRRKHAVLWVLALLTAASAGWARGAEPPPADAITARPMAITSR
                     SMTVKNLDQRVIFDRDVTIVKGDLDLHADRVEVILSRSSAGAAKAPPPTATAGPDVLS
                     EENIDRIVADGRVKVVQGKKTATADHAVYHRAEETIVLTGHPETWEEGVRIQGSKITI
                     LLKEQRSIVEESRVVIYPDAEDGLNSASAESQAPHAR"
     gene            2029..2829
                     /gene="lptB"
                     /locus_tag="AC16BC_03640"
     CDS             2029..2829
                     /gene="lptB"
                     /locus_tag="AC16BC_03640"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR3240740.1"
                     /translation="MPGDPVLTLTNGDASPDALSAVGLEKTFRRRRVVKGVSVTIKPG
                     EVVGLLGPNGAGKTTVFNMIVGVIAPDRGNILLNGRSITALPMHQRARLGVSYLPQEA
                     SVFRKLSVADNVMAILETLPLTREQRQARLQELLEELGVAHLADQKGYTLSGGERRRV
                     EIARALVLSPKFVLLDEPFAGIDPLAVIDVQKVVAHLRSLGIGVLITDHNVRETLEIT
                     QRAYIINNGEILQEGTPVDIASSPVVRSLYLGEHFTWQRSAPAQETTR"
     gene            2826..4292
                     /gene="rpoN"
                     /locus_tag="AC16BC_03645"
     CDS             2826..4292
                     /gene="rpoN"
                     /locus_tag="AC16BC_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR3240741.1"
                     /translation="MRPRLELRLQQKLVMTPQLQQAIRLLQLSRLELSQTITNELMEN
                     PVLEEELSTDAPEDEGPSGEESPAAKTEGETETPEREAPESAELDPQEFNWDSYFEQD
                     DFGLSGEGDYGKASSEDLPSYEQTLSKPTSLEEHLLWQLRLSNVPDEDRVVGETIIGN
                     VDDDGYLRVELEEIAKTAQCSLPKVEQVLKLIQGFDPVGVAARDLRECLQIQIRQLGL
                     QGSLVESIVANHLPDLEKKRFPAIAKSLGVSADAVFQAAKVIEHLEPKPGRPFGSSDN
                     VYITPDVFVVKIEGKYAVLLNDDGLPRLRINQYYRRLLKNKVQGVDPTKTYVEDKLRS
                     ALWLIRSIEQRNRTIVRVAESIVKFQYEFIEKGINFLKPLILKQVAEDIGMHESTISR
                     VTTNKYMYTPQGIFELKFFFNSGISRVNGAGDDLSSVTVRERIRQLVANEDPRHPLTD
                     HELVERLSGEQIAIARRTVAKYRGWLRIPPANQRKRAF"
     gene            4338..4859
                     /gene="hpf"
                     /locus_tag="AC16BC_03650"
     CDS             4338..4859
                     /gene="hpf"
                     /locus_tag="AC16BC_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR3240742.1"
                     /translation="MKTTVTGRHIEITPGLRTHVEERVEHLARYSGRATEATVVLSVE
                     KYRHQAEVSLNVNGVLIQAKEETSEMYASIDEAVAKIERQLKRYKDRQQTRRQRVSTK
                     SRPVEAAEPSQPPASIERERPRLKAMTPDQAAAALDGGRTPLIVFRHAETDATNVVYR
                     RGDGSVAWIDPAP"
     gene            4838..5767
                     /gene="rapZ"
                     /locus_tag="AC16BC_03655"
     CDS             4838..5767
                     /gene="rapZ"
                     /locus_tag="AC16BC_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR3240743.1"
                     /translation="MDRPRAVAESEPAGPPRSEPSRGPRIVIVTGLSGAGKSHVLHCF
                     EDVGFFCVDNLPPPLIPTFVHLYTRGEPTNYRIALGIDIRGREFLSQSFTAIEQLRQS
                     GFCVEMLFLDARDDVLIRRFSETRRPHPLAHARPVSEGIVQEREELRELRDRADLIID
                     TSDYSVHQLKAVIAQRYVERRHGLAVTVISFGYKFGVPREADLVFDVRFLKNPNFVEA
                     LRPLSGQDSAVSAYVVNLPETAGFLARLEDLLDFLLPLYESEGRTYLTIGIGCTGGRH
                     RSVAVAEQLAGLLAQRGLHPTVQHRDIDRQIAG"
     gene            5779..6399
                     /locus_tag="AC16BC_03660"
     CDS             5779..6399
                     /locus_tag="AC16BC_03660"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR3240744.1"
                     /translation="MASYTVAITGASGAIYGVTLLGRLAAAGHEVALVVSPDGVTVIR
                     EETGLDWGGEATVVRRALDRRYRGRVTWWEPANFYAPIASGSHLSDGMIIAPCSMKTA
                     AAVAHGLSASLIERAADVMLKERRPLIVVPRETPLSSIHLENLLTLSRAGAHVVAAMP
                     AFYHRPKSIDDVVAFVVGRVLDHLGIPHDLVPRWGSDRPPRRTCER"
     gene            6389..6916
                     /locus_tag="AC16BC_03665"
     CDS             6389..6916
                     /locus_tag="AC16BC_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240745.1"
                     /translation="MSAEPARPLIPDDQPVSDLEADAEGAVPDTAADGEEHLTLVQRV
                     SRMNVAQKVKFAFRADKEGRTLLLKESSRLVVMAVLNSPKITEQEIETLAKSRNVSEE
                     ILRAIARRREWMAKYSIKHGIAGNPRTPISIAMSQLPLLTTRDLNFLAKDKGVPEPVR
                     NAASRLAKQRSERGG"
     gene            complement(6930..>7297)
                     /locus_tag="AC16BC_03670"
     CDS             complement(6930..>7297)
                     /locus_tag="AC16BC_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240746.1"
                     /translation="QYKRIIGVELSDLIYVARQNVDKMKHRRTRQIDLIQCDAQDFTV
                     PSEVNVIYFYSPFAGPLLEKVTANIYASFRKTLRRIFIIYFNNDHFDRVINRQNWLTK
                     IDQSSLHPHNSCGLYVTRP"
BASE COUNT         1354 a         2265 c         2444 g         1234 t
ORIGIN      
        1 acgcggcgtt cgacgcgatc gaagcctggc tgcgggtcac gggacggtcc gtggtctatc
       61 gcgtatggca gcggcgtcgg gctccggata ccgccacgta catcggtcgg ggcaaggcgg
      121 tccaactgcg ggagttgtgc gagcgggctg gcgtcgacgg cgtcctgatc aacggcacgc
      181 tgagtcgctc gcaacgagac gggttgctcc ggctgctcga ccgaccggtg tgggatcgca
      241 cggtctgggg cgcgacgcag aaaccgccgg tcccggtttc gcgcgcgcgg gaattggcgc
      301 gggcggcgcg gcgccgaaac gggacgttca ccgtggttct gtgcggcgcg gccggcgcgg
      361 ggaagtccac gttgttcgcg gcgctgaccc gcggcgcggt gtcgccgcct tcgtcgtggc
      421 cggtcgggcc ccgagcggcc ggtccggggg tggtcagcag acggttgcga ttcgcgccgg
      481 ggaccggtga cgtggtggtg accgacacgc ccgggctgat ccggaatccg gattcggccg
      541 cgtggatcgt tccggacgaa accctggacg aatgtcgcgg cgcggacctg gtggtccacg
      601 tcatcgacgc gtcgcacccc gaggcgaacc gacgggcgcg ggaggtggac gcggcgctgg
      661 tccgcgcggg tattcacccc gcgctgtcgg tccggacgca ggccgaccgg atctcgaccg
      721 ggtccgacgg gatcgcgccg gggctcgcat cgggccggac cgggtgcgga tgcgacgcgt
      781 tgcgggcgct ggtccggcgc atggccgcgc atcgcgcccc acggctcgtc cggccgcgcg
      841 ggtttatgtt atagtgagcc tcggtgcgtc cggaatgacc aacaacatgg ccgcacgttg
      901 gacggcgtgg agggcccttc cgctctggct gctgatcatc gggggactgg tcgccctggg
      961 agtcgtgctg gtatcgcacg ttcgcgtccg cgggacggcg gtgcccttgt cgccgtcccg
     1021 ggtcgacggc gacctcatcc tcacgggctt cagtctctcc acgttgctca acggcgaacg
     1081 cgagtgggag gtgtcggcgg ctcgggcgcg cctcttcgaa cccgaccatc acgcggtgct
     1141 tgacgaagtg cgcggcacgg tgcggctgcg caatggatcc ttgatgcagt ttgaaggagt
     1201 ctcggcaatt ttcgataccg tgagccatga cctcgatctc gaggggcagg acctcggcgc
     1261 cgtggtgacg ttacccaatg gattcgtcct tcgggccaac cggttgcgct ggacccagga
     1321 acagggcgag ttgtcgtccg acgattcgat cgtgctggtc ggccctcggc tgtccatccg
     1381 gggcgtggga cttcgcgcct ggccgtcgtc ccaggaattc accattctga acagcgtgcg
     1441 cgcggatgtg tcgtcgtaaa cacgccgtac tctgggtgct ggcgctcctg accgcggcgt
     1501 ccgccgggtg ggcgcgaggc gcggagcccc ctcccgcgga cgcgatcacg gcacggccga
     1561 tggccatcac gtcgcgcagc atgaccgtca aaaacctcga tcaacgggtg atcttcgatc
     1621 gtgatgtgac gattgtaaaa ggtgatttag acctccatgc cgaccgcgtg gaggtcatcc
     1681 tctcgcgatc atccgccggc gccgccaagg ccccgccgcc gaccgcgacc gccgggcccg
     1741 acgtcttgtc ggaagagaac atcgatcgga tcgtggcgga tggccgcgtc aaggtggtgc
     1801 aaggcaaaaa aaccgccacg gcggatcatg cggtctacca tcgcgcggaa gaaaccattg
     1861 tcttgaccgg acatcccgaa acgtgggagg agggagtccg catccaaggg tccaagatca
     1921 cgatcctcct gaaggaacaa cggagcatcg tggaagagag ccgcgtggtc atctacccgg
     1981 acgcggaaga cgggttgaat tcggcgtccg cggagtccca ggcaccccat gcccggtgat
     2041 ccggttctca ctctgaccaa cggcgatgcg tcgcccgacg ccctctcggc ggtcgggctg
     2101 gagaagacgt ttcgccggcg tcgggtggtc aagggcgtgt cggtgaccat caagcccggc
     2161 gaggtggtcg ggttgctggg gccgaacggg gcgggcaaga ccacggtctt caatatgatc
     2221 gtgggcgtga ttgcgccgga ccgcgggaat atcctgctga acggccgatc catcaccgcg
     2281 ttgccgatgc accagcgggc gcgcctcggc gtgagttatt tgccgcagga ggcgtcggtc
     2341 tttcggaagc tcagcgtcgc cgacaacgtg atggcgattc tcgagaccct gccgctgacg
     2401 cgcgagcagc gccaggcccg gctccaggaa ttgctggagg aactcggggt cgcgcacttg
     2461 gcggaccaga aggggtacac gttgtcgggc ggcgagcgac gccgggtcga gatcgccagg
     2521 gcgctggtgt tgtcccccaa gttcgtgctg ttggacgaac cgttcgcggg gatcgatccg
     2581 ctggcggtca tcgacgtcca aaaggtcgtg gcccacttgc gcagcctcgg aatcggcgtc
     2641 ctgatcacgg atcacaacgt gcgcgagacg ctggaaatca cgcagcgagc gtatatcatt
     2701 aacaacggcg agatcctcca ggaaggcacc ccagtggaca ttgcgtcgag cccggtcgtg
     2761 cgatccctgt atcttggcga gcatttcacg tggcaacgct ccgcccccgc gcaggaaacc
     2821 acccggtgag accgcgtctc gaactgcggc tgcaacaaaa gctggtgatg acgccgcagt
     2881 tgcagcaggc gatccgcttg ttgcagcttt cccgcctgga gttgagccag accatcacca
     2941 acgaactgat ggagaatccg gttctcgaag aggagttgtc gaccgacgcg ccggaggacg
     3001 agggacccag cggcgaggag tcgccggcgg cgaaaaccga aggcgaaacc gagacgcccg
     3061 aacgcgaggc cccggaaagc gccgagctgg acccccagga attcaactgg gacagctatt
     3121 tcgaacaaga cgatttcgga ttgtcggggg agggcgacta cggcaaagcc tcctccgaag
     3181 atctcccctc atacgaacag accctcagca aacccacgtc gctggaagaa cacctgttat
     3241 ggcagcttcg gctgtcgaac gtgcccgacg aagaccgggt ggtgggtgag acgatcatcg
     3301 ggaacgtgga cgacgacggg tatctgcgcg tggaactcga agagatcgcg aagaccgcgc
     3361 aatgctcgtt gcccaaagtc gagcaggtct tgaaactgat tcagggattc gacccggtgg
     3421 gggtcgcggc gcgggacctg cgcgaatgcc tgcagattca gatccgacaa ctgggcctcc
     3481 agggctcgct cgtcgagtcc atcgtggcca accacctgcc ggatttggaa aagaagcggt
     3541 ttcccgcgat cgccaaatcg ctgggcgtgt ccgcggacgc ggtgtttcag gccgcgaaag
     3601 tcatcgaaca tcttgaaccc aagccgggcc gcccgttcgg atcgtccgac aacgtctaca
     3661 tcacgccgga cgtgtttgtc gtgaagatcg agggcaaata cgccgtgctg ctgaacgacg
     3721 acggcctgcc gcggctgcga atcaatcagt actaccgccg tctgctcaaa aacaaggtcc
     3781 aaggcgtgga cccgaccaag acctacgtcg aggacaaact ccgatccgcg ttgtggctca
     3841 tccgcagcat cgaacagcgc aaccgcacga tcgtgcgcgt ggccgagagc atcgtgaaat
     3901 tccagtacga attcatcgaa aaaggcatca attttctcaa gccgttgatc ctcaagcagg
     3961 tcgcggaaga catcggcatg cacgagtcca cgatcagtcg cgtcacgacc aataaataca
     4021 tgtatacgcc gcaggggatt ttcgagctca agtttttctt taacagcgga atcagccgcg
     4081 tgaacggggc gggcgacgat ctctcgtcgg tcacggtgcg cgaacggatc agacaactgg
     4141 tcgcgaacga agacccgcga catccgttga cggaccacga actggtcgag cggctctcgg
     4201 gcgaacaaat cgccatcgcg cgccgcaccg tggccaaata ccgcggctgg ttgaggattc
     4261 cccccgcgaa tcagcgcaag cgcgcgtttt aagcgggaca agaccacccg gtatccacgg
     4321 aaggagcaat cggcacgatg aagacgacgg tgacagggcg gcacatcgaa atcacccccg
     4381 gacttcgcac gcacgtggaa gagcgcgtcg aacacctggc tcggtactcc ggccgggcga
     4441 ccgaggccac ggtcgtcctg tcggtggaaa agtaccggca ccaagccgag gtgtccttga
     4501 acgtgaacgg ggtgctgatt caggccaaag aagaaacctc ggaaatgtac gcctcgatcg
     4561 atgaagccgt cgccaagatc gaacgccaac tgaaacgata caaagaccgt cagcagacgc
     4621 gccgccaacg cgtgtccacg aagtcccgac cggtcgaagc cgcggaaccg tcgcaaccgc
     4681 ccgcgtcgat cgagcgcgag cggccgaggc tcaaggccat gacgcccgac caagcggccg
     4741 cggcgttgga cggagggagg acgcccttga tcgtgttccg ccacgcggaa acggacgcga
     4801 ccaacgtggt gtatcgccgc ggcgacgggt cggtggcatg gatcgacccc gcgccgtagc
     4861 ggagtccgaa cccgccgggc ctccgcgctc ggaaccgtcg cggggccccc ggatcgtgat
     4921 cgtcaccggc ctgtccggcg cgggcaagag ccacgtcctg cactgtttcg aggacgtggg
     4981 attcttctgc gtcgacaatc tcccgccgcc gctcattccg acgttcgtcc atctgtacac
     5041 gcggggcgaa cccacgaatt accgcatcgc gctcggcatc gacatccgcg gccgcgagtt
     5101 tctctcgcag agctttaccg cgatcgaaca actccggcag tcggggtttt gcgttgaaat
     5161 gttgttcctc gatgcgcgcg acgacgtgtt gatccggcgc ttctccgaga cccgccgacc
     5221 gcatccattg gcccatgccc ggccggtcag cgagggcatc gtgcaggagc gggaggaact
     5281 ccgggaattg cgcgatcggg ccgatctgat catcgacacc tcggactact ccgtccacca
     5341 actcaaggcg gtcattgcgc agcggtacgt ggagcgcagg cacggtctgg ccgtcaccgt
     5401 catctcgttc ggctacaagt tcggcgtgcc gcgggaggcg gatctggtgt tcgacgtgcg
     5461 attcctcaag aaccccaatt tcgtggaagc gctccgcccg ctgtccggcc aggactccgc
     5521 cgtgtccgcc tacgtcgtga acctacccga aaccgcgggg ttcctggcgc ggctggagga
     5581 cctgctcgat tttctgctcc ccctctatga aagcgagggc cggacgtatc tgaccatcgg
     5641 catcgggtgc acgggcggcc gtcaccgatc cgtggcggtg gccgagcaac tggcgggcct
     5701 gctggcgcag cggggattgc acccgaccgt ccagcatcgg gatatcgacc ggcaaatcgc
     5761 gggctgacga ggcgaggcat ggcgtcctat acagtggcga tcaccggggc aagcggcgcc
     5821 atctacggcg tgacgttgct gggacggttg gccgccgccg gtcacgaggt ggcgttggtg
     5881 gtgtcgccgg acggcgtgac cgtgatcagg gaggaaaccg ggctcgactg gggcggggag
     5941 gcgaccgtcg ttcgtcgcgc gctcgatcgc cgctaccggg gtcgggtcac ctggtgggag
     6001 cccgcgaact tctacgcgcc catcgccagc ggctcgcacc tgagcgacgg catgatcatc
     6061 gcgccctgct cgatgaaaac cgcggccgcg gtggcgcacg gcctctccgc gtcgttgatc
     6121 gagcgggccg cggacgtgat gctcaaggag cggcgcccgt tgatcgtggt gcctcgcgaa
     6181 acgccgctct cctcgattca cctggagaat ctgctaacct tgtcgcgggc gggcgcgcac
     6241 gtggtggcgg cgatgcccgc gttctatcac cgccccaagt cgatcgacga cgtggtggcg
     6301 ttcgtggtcg ggcgggtgct ggaccacctc ggcatcccgc acgatctcgt tccacggtgg
     6361 ggctcggacc gcccgccaag gaggacctgt gagcgctgaa cccgcacgac cgttgatccc
     6421 cgacgatcaa cccgtctccg atttggaggc cgacgccgag ggcgccgtcc ctgacaccgc
     6481 ggccgacggg gaggagcacc tgacgctggt tcagcgcgtg tcgcggatga acgtggccca
     6541 gaaggtgaaa ttcgcgtttc gcgccgataa ggaaggccgg accttgttgc tcaaggaatc
     6601 cagccggctc gtggtgatgg cggtgctcaa ctcgcccaag atcacggaac aggaaatcga
     6661 aacgctggcg aaatcgcgca acgtctcgga agagatcctg cgcgccattg cgagacggcg
     6721 cgagtggatg gcgaagtact ccatcaaaca cgggatcgcc ggcaacccca ggaccccgat
     6781 ttcgatcgcg atgagtcagc taccgctgct cacgacgcgc gatctgaatt ttctcgccaa
     6841 ggacaagggc gtgcccgaac cggtccggaa cgccgcgtcg cgactggcga agcagcgcag
     6901 cgaacgcggc ggctaagact ccgtggccct cacggacgcg tgacgtagag gccgcacgaa
     6961 ttgtgcgggt gtaggctgga ctggtcgatt ttggtcagcc aattctgacg gttaattact
     7021 cggtcaaagt gatcgttgtt gaagtagatg atgaaaattc tgcgcagagt cttccggaat
     7081 gaagcataga tattcgcggt taccttctcc aagagagggc ctgcaaacgg actgtagaag
     7141 tagatgacgt tgacctctga gggaacggta aagtcctgcg catcgcattg gatcaggtca
     7201 atttggcgcg tccggcgatg cttcatcttg tcgacatttt gcctggcgac atagatcaag
     7261 tcggaaagtt ctaccccgat gatccgtttg tactgac
//
LOCUS       DBNGTT010000162         1826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3100062, whole genome shotgun sequence.
ACCESSION   DBNGTT010000162 DBNGTT010000000
VERSION     DBNGTT010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3100062"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="AC16BC_03675"
     CDS             <1..603
                     /locus_tag="AC16BC_03675"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR3240747.1"
                     /translation="GGVLLAATLVGCDERPAAAPLPESKAQAVREGTRVGFLAPSFTL
                     ERLGGGASSLSEFRGKVVLLNFWATWCGPCRAEMPSLEALSHQFPSQDFMVVGIATDY
                     EGAQIVQPFMESFGLTFPLLLDPEMQVNDRFEIRSLPTSIVLDRRGVIRHKFFGAMDW
                     NTVKNRDLVRVLVGESTGAPAPAAGAGLSRPAPTPSGIEG"
     gene            607..1347
                     /locus_tag="AC16BC_03680"
     CDS             607..1347
                     /locus_tag="AC16BC_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR3240748.1"
                     /translation="MDASVPSFAFAFSAGFLSFVSPCVLPLVPAYVSFVTGLSFDELT
                     RGTDRYRVKWTTLVTSLLFIAGFSTVFILFGASASLMGQVVLTHQKLLRWVGGAVIVF
                     FGLYVMGAFNLPALAADRRLHLQGRPAGYLGAYVVGVAFAAGWTPCVGPILGSVLLLA
                     STKESLWQGVQLLGVYSVGLGLPLLITALGLNTFLSRMKAIGPYMPVVTKASGVLLVA
                     VGILIATNSLSILSSFLTQNGIGVSVGQ"
     gene            1431..>1826
                     /locus_tag="AC16BC_03685"
     CDS             1431..>1826
                     /locus_tag="AC16BC_03685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3240749.1"
                     /translation="MGPSSTYDAIVVGGGPAGASAAYEIATAGRAVLLLERETFPRYK
                     VCGGGLSARLDRVIGTAYHETVEATTRGMEFVYHGGDAFTVDADGPVAHFVMRDRFDA
                     WLVDRARRAGAEVRFGEPVTEIADPDASGV"
BASE COUNT          251 a          545 c          630 g          400 t
ORIGIN      
        1 ggaggcgtgc ttctggcggc cacgctggtc ggctgcgacg aacgccccgc tgccgcgccc
       61 ttgccagaat cgaaagccca ggcggtgcgt gaagggacgc gcgtgggatt tctggcgccg
      121 tcgttcacgt tggagcgtct tggcggcggc gcgtcgtcgc tgagcgagtt ccgaggcaag
      181 gtggtcttac tcaatttttg ggcgacgtgg tgtggtccgt gccgtgcgga aatgccctcg
      241 ctcgaggccc tctcgcacca attcccgtcg caggatttca tggtcgtggg catcgccacg
      301 gattacgagg gcgcccagat cgtccagccg ttcatggagt cgttcggcct cacctttccg
      361 ctcctgctgg acccggagat gcaagtgaac gaccggtttg agatcaggtc gcttccgacc
      421 agcatcgtgt tggatcgccg aggcgtgatc cggcacaagt tctttggtgc gatggattgg
      481 aacaccgtca agaaccggga cctggtgcgc gtgctcgtcg gcgaatccac gggcgcgccc
      541 gcgccggccg ctggcgccgg tttgagccgt ccggcgccga caccttccgg catcgaaggt
      601 tagttggtgg acgcgtccgt tccgtcgttc gcgttcgcgt tttcagcggg atttttgtcc
      661 ttcgtgtcgc cttgcgtgct cccgttggtt cccgcatacg tctcattcgt gacggggctg
      721 tcgttcgatg aactgacgcg cggaaccgac cggtatcggg tgaagtggac gacgttggtc
      781 acgtcgctgt tgttcatcgc cgggttttcc acggtgttta ttctcttcgg cgcgtccgca
      841 tccttgatgg gccaggtggt gctcacccac cagaaattat tgcgatgggt cggcggcgcg
      901 gtcattgtat ttttcgggct ctacgtgatg ggcgcgttca atctgccggc attggcggcg
      961 gatcgacgtc tgcacctaca gggccggcca gcgggctatc tcggcgcgta cgtggtcggg
     1021 gtggcgttcg ccgcggggtg gacgccgtgc gtgggaccga tcctcggatc ggtgttgctg
     1081 ctcgcgagca ccaaagaatc gctctggcag ggcgtgcagt tgttgggcgt gtattcggtt
     1141 gggctgggcc tgccgttgct catcaccgcg ctcggtctca acacctttct gtcgcggatg
     1201 aaagccatcg gcccctatat gcccgtggtg accaaggcga gcggggtgct cctggtggcc
     1261 gtcggcatcc tcatcgccac caattcattg tccatcttga gttcattcct gacgcagaac
     1321 gggatcgggg tgtcggtcgg ccagtaacct gccttcttgg cggcccgatt tccggtttgg
     1381 ccgcgtaacg tcgtagaaca aatggcttac cgggatccat gagcgtccgt atgggccctt
     1441 catccacgta tgacgcgatc gtggtgggcg gcggacccgc gggggcttcc gccgcgtacg
     1501 agatcgcgac cgcgggccgc gctgtcctcc tgctcgaacg cgagacgttc ccccggtaca
     1561 aagtgtgcgg cggtggactc tcggcccggt tggatcgggt catcgggacg gcgtaccacg
     1621 aaaccgtgga ggccaccacg cgaggtatgg agttcgtgta ccacggcggc gacgcgttca
     1681 ccgtggatgc cgatggcccg gtcgcgcatt ttgtgatgcg cgaccgcttt gacgcctggc
     1741 tggtggatcg ggcgaggcga gccggcgccg aggtgcgttt cggcgagccc gtgaccgaga
     1801 ttgccgaccc cgatgcgtcg ggggtc
//
LOCUS       DBNGTT010000163         7204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2333436, whole genome shotgun sequence.
ACCESSION   DBNGTT010000163 DBNGTT010000000
VERSION     DBNGTT010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2333436"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..654
                     /gene="rpsE"
                     /locus_tag="AC16BC_03690"
     CDS             142..654
                     /gene="rpsE"
                     /locus_tag="AC16BC_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR3240750.1"
                     /translation="MERVNPDELTLKDKVVFINRVAKVVKGGKRFSFAAVVVVGDGQG
                     YVGVGKGKAAEVPEAIRKAVEHAKKSLVRVPITEDTIPYETVGRCGAERILMKPAAEG
                     TGIIAGGAVRSVMEISGVRNILCKSLGSGNPYNVVKATLNGLTSLRDPETIARMRGVR
                     VSEAAEVGAS"
     gene            651..857
                     /gene="rpmD"
                     /locus_tag="AC16BC_03695"
     CDS             651..857
                     /gene="rpmD"
                     /locus_tag="AC16BC_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR3240751.1"
                     /translation="MSAKPGFIAIKLVRSPIGRPAKHKLVVKGLGFRKLQQTVIRPDT
                     PAIRGMVYEIRHMVRVEPHTGERA"
     gene            857..1315
                     /gene="rplO"
                     /locus_tag="AC16BC_03700"
     CDS             857..1315
                     /gene="rplO"
                     /locus_tag="AC16BC_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3240752.1"
                     /translation="MRLDQLAPASGAKKKEKRIGRGIGSGHGKTSTKGHKGQGARSGG
                     TKAPGFEGGQNPIIRRAPKRGFVNIFRQAPAVVNLDRLAGLDASQGVTPERLKAEGVI
                     KASAQTVKILGDGTLSAPLTVQAHAFSRSAKEKIEAAGGKAVLIETATRG"
     gene            1330..2643
                     /gene="secY"
                     /locus_tag="AC16BC_03705"
     CDS             1330..2643
                     /gene="secY"
                     /locus_tag="AC16BC_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3240753.1"
                     /translation="MFERLLTNLQNVFKIPELRQRILFTLGMLAVYRVAAHVPTPGIN
                     GEQLSTFLHQQGGALMGFLDMFSGGALAKVTIVALGIMPYISASIILQLLTVVVPSLT
                     KLAKEGERGRKKIIQYTRYGTIVIGLVQSFGIAVGLEKMNGGAFVQHPGWSFRLLTMV
                     TITAGTAFLMWLGEQITERGIGNGISLIIFSGIVANVPNAMIKTYSLWDTGQLNSLFL
                     LALTIMMVGVTAAIVFLESGRRKIPVQYAKRVVGRKVYGGQNTHIPLKINTAGVIPPI
                     FASSLIAFPATIAGFIQIAWVQSLSQQLAPGSLLYTILYVTLIIFFAFFYTAVVLNPV
                     DMADNMKKYGGFIPGIRPGQKTSDYIYKVLTRVTFAGSVYLAVVCVIPELLIYQAGLP
                     FFFGGTSLLIVVGVGLDTAQQIETHMITRNYEGFMRKGRLKGRSG"
     gene            2849..3505
                     /locus_tag="AC16BC_03710"
     CDS             2849..3505
                     /locus_tag="AC16BC_03710"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3240754.1"
                     /translation="MRVVFIGPPGVGKGTQAQRLAAAKGIAKISTGDVLREAVRNGTP
                     LGKKAKAFMDSGALVPDDLVVDMLAERLKAADTKPGYLLDGFPRTLAQAEALEKMLAS
                     RGERLDQAVAFEAAADVIVARLSGRRSCPACGKVYHVSNDPSPRGSACGACGTELVQR
                     DDDREDTIRKRLTVYERETSPVLSYYQGRGLLTRLDGTAPIDQVAARVEAALGGRRSA
                     "
     gene            3502..4254
                     /gene="map"
                     /locus_tag="AC16BC_03715"
     CDS             3502..4254
                     /gene="map"
                     /locus_tag="AC16BC_03715"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3240755.1"
                     /translation="MIILKTPQEIALIAKASRVVALTIAFLRERVKPGITTADLDRWA
                     EEFIVREGARPAFKGYRGYPATLCTSVNEEVVHGIPSTRKRLREGDIIGVDVGAVVEG
                     FHGDAAVTLPVGEISEEAARLIRVTDASLAAGLAQVKVGNRLSDVSHAIQTVAEGEGY
                     SVVTDFVGHGIGRNLHEDPQVPNFGRPGEGPRLKEGLVLAIEPMVNAGRSEVEVLADR
                     WTVVTKDRKLSAHFEHTIALTADGPKVLTLAA"
     gene            4482..4703
                     /gene="infA"
                     /locus_tag="AC16BC_03720"
     CDS             4482..4703
                     /gene="infA"
                     /locus_tag="AC16BC_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3240756.1"
                     /translation="MPKEDTIEVQGTIVEPLPNAMFRVALENGHKVLAHISGKMRMHY
                     IKILPGDKVTVELSPYDLTRGRITYRFSK"
     gene            4718..4831
                     /gene="rpmJ"
                     /locus_tag="AC16BC_03725"
     CDS             4718..4831
                     /gene="rpmJ"
                     /locus_tag="AC16BC_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3240757.1"
                     /translation="MKVRSSVKAICAKCKIIKRAGVVRVICDNPRHKQRQG"
     gene            4841..5218
                     /gene="rpsM"
                     /locus_tag="AC16BC_03730"
     CDS             4841..5218
                     /gene="rpsM"
                     /locus_tag="AC16BC_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3240758.1"
                     /translation="MARISGIDLPRDKRVDIGLTAIYGIGRSSALKIVRDAKVDPATR
                     IKALQEAEVVRLREIIDRDYKVEGDLRRETAMNIKRLMDIGCYRGLRHRRGLPVRGQR
                     THTNAKTRKGPNRRGIGAKKKEK"
     gene            5300..5686
                     /gene="rpsK"
                     /locus_tag="AC16BC_03735"
     CDS             5300..5686
                     /gene="rpsK"
                     /locus_tag="AC16BC_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3240759.1"
                     /translation="MVNKKVGKKKERKHVQTGVAHVQASFNNTIVTVTDLAGNVVVWA
                     SAGNQGFKGSRKSTPFAAQRAADTAAKKAMEHGMRQVDVYVKGPGSGRESAVRALQAA
                     GLKINLIRDVTPIPHNGCRPPKRRRV"
     gene            5751..6377
                     /gene="rpsD"
                     /locus_tag="AC16BC_03740"
     CDS             5751..6377
                     /gene="rpsD"
                     /locus_tag="AC16BC_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3240760.1"
                     /translation="MGRYVGPVCRLCRREGTKLFLKGTRCYTDKCAVDRRGYAPGQHG
                     QGRMRTSEYSLQLREKQKLKRIYGLLERQFRGYFHRAERMKGITGENLLVLLERRLDN
                     VMYRMGFGASRREARQLVLHGHCLVNGKKVNIPSFLVLPGATVSIHESSRGLLPIQKA
                     LEAVDGRGIPSWLEVDKTHCTGRVKELPTKEEIALPVNEQLVVELYSR"
     gene            6429..>7204
                     /locus_tag="AC16BC_03745"
     CDS             6429..>7204
                     /locus_tag="AC16BC_03745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3240761.1"
                     /translation="MATMIKMNDFQAPRKLECDKESLTDTYGKFTAEPFERGYGTTIG
                     NALRRVLLSSIVGAAVTSIKFEGVQHEFSTIPGVKEDVTDVVLNLKRLRVKLHGDKPK
                     TITISKKGPGDLTGEDIIHDADVEILTPDVHIATLDKDAHVKAELTVKYGRGYVPADR
                     NKEEGMPIGTIPIDAIFTPVKAVNFVIEPARVGRITDYDRLVMEVWTDGSITPEDALG
                     HASKIVKDHMQIFINFDEGDEVAMPVTEEARQDLNRNLFRG"
BASE COUNT         1442 a         2020 c         2529 g         1213 t
ORIGIN      
        1 ggttcatggg ttcttgagtt taaatggttc gagggggcaa agaggaaaag gggttggaaa
       61 cgacggacca agggttcgcg tcgttgaact ccttgaactc gttgaaccac ttgaacgtgt
      121 tttcacaacg ggggtgaggg tttggaacgg gtaaatccgg acgagctgac gctgaaagat
      181 aaggtcgtct tcatcaaccg cgtggccaag gtggtcaagg gcggcaaacg attcagtttc
      241 gccgcggtcg tggtggtggg ggacggccaa gggtacgtcg gggtcggcaa aggcaaggcg
      301 gccgaggttc ccgaagccat ccgcaaggcc gtggagcacg cgaaaaagag cctggtgcgg
      361 gtgccgatca ccgaagacac catcccgtat gagacggtgg gccggtgcgg cgccgagcgg
      421 attctcatga agcccgcggc ggaagggacg ggcatcatcg cgggcggcgc ggtgcggtcg
      481 gtgatggaga tctcgggggt ccggaatatt ctctgcaagt cgttgggcag cgggaatccg
      541 tacaacgtgg tcaaggcgac gctgaacggt ctcacgtcgc tgcgcgatcc ggagacgatc
      601 gcccggatgc ggggcgtgcg cgtgagcgag gccgccgaag tgggggcatc atgagcgcga
      661 aaccgggatt catcgccatt aagctggtgc gaagccccat cggtcgtccc gccaagcaca
      721 aattggtggt caagggcctg ggatttcgca agctgcagca gaccgtgatc cgtcccgaca
      781 ctcccgcgat tcgtgggatg gtgtacgaga ttcgtcatat ggtgcgggtt gaaccccaca
      841 ccggagagcg ggcatgattc gattggatca actggctccg gccagcggcg caaagaaaaa
      901 agagaagcgg attgggcgcg ggatcgggtc cgggcacggc aagacctcga ccaagggcca
      961 caagggccag ggcgcccgct ccggcggcac caaggcgccc gggttcgagg gcggacagaa
     1021 cccgatcatt cggcgcgcgc ccaagcgcgg cttcgtgaat atcttccggc aggctccggc
     1081 cgtggtgaat ctcgaccggt tggccgggct ggacgcgtcg cagggcgtga ccccggagcg
     1141 gctcaaagcc gagggggtga tcaaggcgtc ggctcaaacc gtcaagatct tgggggacgg
     1201 caccttgtcc gcgccgttga ccgtgcaggc ccatgcattc agccggagcg ccaaagagaa
     1261 aatcgaggcg gccggaggaa aggcggtgtt gatcgaaacc gcgactcgtg gttaagaagg
     1321 aacgcccgcg tgtttgaacg gctcctcacc aacctgcaaa acgtcttcaa gatccccgag
     1381 ctccgacagc ggattctgtt cacgctgggg atgctggcgg tctatcgcgt cgcggcccac
     1441 gtgcccacgc cgggcatcaa cggcgaacaa ctgagcacat tcctccatca gcagggcggg
     1501 gcgctgatgg ggtttttgga tatgttctcc ggcggcgcgt tggcgaaggt gaccatcgtg
     1561 gcgctgggga tcatgccgta tatctccgcg tcgatcatcc tgcagttgct gacggtggtc
     1621 gtgccgtcgc tgaccaagct ggcgaaagaa ggggaacgcg ggcgcaagaa gatcatccag
     1681 tacacgcgct acggcaccat cgtcatcggg ttggtccaat cgttcggcat cgcggtcggg
     1741 ttggagaaga tgaacggggg cgcgttcgtc cagcaccccg ggtggtcgtt ccggttgctg
     1801 accatggtca cgatcacggc cgggaccgcg ttcctcatgt ggctcggcga gcagatcacg
     1861 gagcgcggga tcggcaacgg gatttcgttg atcattttct cgggcatcgt ggcgaacgtg
     1921 cccaacgcga tgatcaagac gtacagtctg tgggacacgg gccaattgaa cagcctcttt
     1981 ttgctcgcgc tcacgatcat gatggtgggg gtgacggccg cgatcgtgtt tctggaaagc
     2041 ggacggcgca agattccggt gcagtacgcc aaacgggtcg tgggccggaa agtgtacggg
     2101 gggcagaaca cgcacatccc cctgaagatc aacaccgcgg gcgtgatccc gccgattttc
     2161 gcgtccagct tgatcgcgtt tcccgcgacc atcgcgggat tcatccagat cgcgtgggtg
     2221 cagagcctgt cgcagcagtt ggcgccgggc tcgctgctct atacgattct gtacgtgacg
     2281 ttgattattt tcttcgcgtt cttctacacc gccgtggtgt tgaacccggt ggacatggcg
     2341 gacaacatga agaagtacgg cggcttcatc cccgggattc gtccggggca gaagacctcc
     2401 gattacatct ataaagtcct cacgcgcgtt acgttcgccg ggtcggtgta cctggcggtg
     2461 gtgtgcgtga tccccgagct gctgatctac caggccgggc tgccgttttt cttcggcggc
     2521 acgtcgctcc tgatcgtggt cggcgtgggc ttggacaccg cgcagcagat tgaaacgcac
     2581 atgatcaccc gcaactatga aggcttcatg cggaagggcc ggctcaaggg ccggagcggg
     2641 taagccgggc gatgaatggc catctgctgc gttgccgttg cgcgtccggt gctcacgtac
     2701 tcacaacgta cgctccgctc cggtgctcac ggcgtcttgc atctggcgca ttgctccgcc
     2761 acctgcaagg aatggacagc caagttggca ctcgcgtcgt tgacgcgtcc aggttcctca
     2821 cccggcttga tcggaagggc cgatagggat gagagtcgtg tttatcggcc ctcccggcgt
     2881 gggcaagggc acgcaggcgc agcggttggc cgcggccaag ggcatcgcca agatttcgac
     2941 cggcgacgtg ctgcgggaag cggtccgaaa cggcacgcca ttggggaaga aggccaaggc
     3001 gttcatggat tccggcgcgc tggtccccga cgacctggtg gtcgacatgt tggccgaacg
     3061 tttgaaggcg gccgacacca agcccggcta tttgctcgac ggctttcccc ggacgctggc
     3121 gcaggccgag gcgctcgaaa agatgttggc gtcgcgcggg gagcgattgg accaggcggt
     3181 ggcgttcgaa gcggcggcgg acgtgatcgt ggcgcggttg tcggggcggc gatcgtgtcc
     3241 cgcgtgcggc aaggtctatc acgtgagcaa cgacccgtcg ccgcgcggca gcgcgtgcgg
     3301 cgcgtgcggg accgagctgg tccagcggga cgacgaccgg gaagacacga ttcgcaaacg
     3361 attgaccgtg tacgagcgtg aaacttcgcc cgtattatcg tattatcagg ggcgcggatt
     3421 gctgacccga ctcgacggga cggcgccgat cgaccaggtg gcggcgcgcg tcgaggcggc
     3481 gctcggcggt cgccggtcgg catgattatt ttgaagacgc cgcaggaaat cgcgttgatc
     3541 gccaaggcca gccgggtcgt ggcgttgacg atcgcgtttc tgcgcgagcg ggtcaagccg
     3601 ggcatcacca ccgcggattt ggaccgctgg gccgaggaat tcatcgtgcg cgagggcgcc
     3661 cggcccgcgt tcaagggcta ccgcgggtac ccggccacct tgtgcacctc ggtgaacgaa
     3721 gaggtggtgc acgggatccc gtcgacgcga aagcgtctgc gcgagggcga catcatcggc
     3781 gtggacgtgg gcgccgtggt cgagggattt cacggcgacg cggcggtcac gctgccggtc
     3841 ggggagatct cggaagaggc cgcgcggctg atccgcgtga ccgatgcgtc gttggcggcc
     3901 ggcctggccc aggtgaaggt gggcaatcgg ctgtcggacg tgtcgcacgc gatccagacc
     3961 gtggccgaag gcgagggata ttcggtggtc acggatttcg tgggccacgg tatcgggcgc
     4021 aatctccatg aagacccgca ggtcccgaac ttcggccggc ccggcgaggg gcctcgcttg
     4081 aaagaggggc tggtgctggc gatcgaaccc atggtgaacg ccggacgcag cgaggtcgaa
     4141 gtgttggcgg accggtggac cgtggtgacc aaggaccgca agttgtcggc gcactttgag
     4201 cacacgatcg cgctgaccgc agacggtccc aaagtgttga cgctggcggc gtagtaatta
     4261 ggatgctgaa aaggtcgaag ggaaattcag gctgctcaaa aagctccggc tgcaaggccg
     4321 cagggagaag gaaaccggag cgtactgggt cgtacgtgag gatttccgac gaccgagaat
     4381 ctgcgccgcg ccaaaggcgt ggtatggcaa tgatcgcttc cctacagatg gcgaagcaac
     4441 gcggcagccg gactttttca gcagccggct aacgaggatt gatgccgaag gaagacacga
     4501 tcgaagtgca gggcacgatt gtcgaaccgc tccccaacgc catgttccgc gtggccttgg
     4561 agaacggaca caaggtgctg gcgcatatct cgggcaagat gcggatgcac tacatcaaga
     4621 ttcttcccgg ggacaaagtg acggtggaac tctcgcccta cgatttgacg cgggggcgaa
     4681 ttacgtaccg gttcagcaag taaggacgag ggacgacatg aaagtacggt catcggtcaa
     4741 ggccatttgc gcaaagtgca agatcatcaa gcgtgccggc gtggtgcggg tcatctgcga
     4801 taacccccgg cacaagcagc gacagggata ggggacgagt atggcgcgta tttctggaat
     4861 cgatttgccg cgggacaagc gggtcgacat cgggctgacc gcgatctacg ggatcggtcg
     4921 gtcgtcggcg ctcaagatcg tgcgcgacgc gaaagtggat ccggcgacgc ggatcaaagc
     4981 gcttcaggaa gccgaagtgg tgcgtttgcg cgaaatcatc gaccgcgact acaaggtcga
     5041 gggcgatctg cgacgcgaga ccgcgatgaa catcaagcgc ttgatggaca tcggctgtta
     5101 ccgcggcttg cggcaccggc gcgggctgcc ggtgcgcggc cagcgcacgc acacgaacgc
     5161 caagacccgc aagggtccca accgacgcgg gatcggggcg aagaaaaaag agaagtaggg
     5221 ccggaagcgt ctcgctgctc ggcccgggaa ggcgtggcct gcgcggcgtt tcgcgagcga
     5281 ggaagcgagg atctgacgga tggtgaacaa gaaggtcggc aagaagaaag aacgcaaaca
     5341 cgtgcagacg ggcgtggccc acgtccaggc ctcgttcaac aacacgattg tgacggtgac
     5401 ggacctggcc ggcaacgtgg tggtgtgggc gagcgcgggg aaccagggct tcaagggctc
     5461 gcgcaagagc acgccgttcg ccgcgcagcg ggccgcggat accgccgcca aaaaagcgat
     5521 ggaacacggg atgcggcagg tggacgtgta cgtcaagggg ccggggtccg ggcgggagtc
     5581 ggcggtgcgc gcgttgcagg ccgcgggctt gaagatcaac ttgatccgcg acgtgacgcc
     5641 gattccgcac aacgggtgtc ggccgcccaa acgtagaagg gtctagtaca acgtcgaacc
     5701 ggggagccgg agagccggcg aaccggggag tacacaatta ggagaagagc gtgggacgtt
     5761 atgtcgggcc ggtgtgccgg ctgtgccgtc gtgaaggcac gaaactgttt ctgaagggca
     5821 cgcgctgtta cacggacaag tgcgccgtgg accgccgcgg ttacgccccg ggccagcatg
     5881 ggcagggccg gatgcgcacc tcggagtaca gcctccagct gcgtgaaaag caaaaactca
     5941 agcgcattta cggcctcctg gagcgccagt tccgcgggta cttccatcgg gcggagcgca
     6001 tgaagggcat caccggcgag aatttgctcg tgttgcttga acggcgtttg gacaacgtca
     6061 tgtaccggat gggattcggc gcctcgcgcc gcgaggcccg gcagttggtg ttgcacggcc
     6121 attgcctggt caacgggaaa aaggtcaata tcccgtcgtt tctggtgctg cccggcgcga
     6181 ccgtctcgat tcacgagtcg tcccgaggcc tgctgccgat ccagaaggcg ctggaggccg
     6241 tggacgggcg cgggattccg agctggcttg aggtggacaa gacccactgc acggggcggg
     6301 tgaaggaact gccgaccaag gaggagattg ccctgccggt caacgagcag ctcgtcgttg
     6361 agctgtactc gcgataagtc gattgacggc acgcgatggc gaacgcaggc tagggtgggg
     6421 agaattctat ggcaaccatg atcaaaatga atgattttca ggcaccgcgg aaactcgaat
     6481 gcgacaaaga gagcttgacc gatacgtacg gcaagttcac ggcggagcct tttgagcgcg
     6541 gatacgggac cacgatcggc aacgcgctgc gtcgcgtgct gctctcgtcg atcgtgggcg
     6601 ccgccgtcac ctcgatcaag ttcgagggcg tgcagcacga attctcgacg attcccgggg
     6661 tcaaagagga cgtcaccgac gtggtcctga acctcaaacg gctgcgcgtg aagctgcacg
     6721 gggacaagcc caagacgatc acgatcagca aaaagggccc cggcgatttg accggcgagg
     6781 acatcatcca cgacgcggac gtggagatcc tgacgcccga cgtgcacatc gcgaccctgg
     6841 acaaggacgc gcacgtgaag gcggagttga ccgtcaaata cggccgcgga tacgtcccgg
     6901 cggaccggaa caaggaagag ggcatgccga tcgggacgat cccgatcgac gcgattttca
     6961 ccccggtcaa ggccgtgaac ttcgtgatcg agcccgcgcg cgtgggtcgg atcacggact
     7021 acgatcgcct ggtcatggaa gtctggaccg acggcagcat caccccggag gacgcgctgg
     7081 gccacgcgtc gaagatcgtc aaggatcaca tgcagatctt catcaacttc gacgaaggcg
     7141 acgaagtggc catgccggtc accgaagaag cgcgccagga cctcaaccgg aacttgttcc
     7201 gcgg
//
LOCUS       DBNGTT010000164         7069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1439307, whole genome shotgun sequence.
ACCESSION   DBNGTT010000164 DBNGTT010000000
VERSION     DBNGTT010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1439307"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..1215
                     /locus_tag="AC16BC_03750"
     CDS             445..1215
                     /locus_tag="AC16BC_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3240762.1"
                     /translation="MVARRTSLMTSTLSRRSSLRPMVILSGALHAVFAVLLVWGPSWI
                     PRRPAMPVYEVQLVSVPVEAPRPPRQPPPPRVEPKPPPPPPKPVKAKPVKKPVQAAVE
                     PAPTKAHPAPAPVPEPAKTPPQPERQPDPPAPEPQAKAAEPTEPGIQLMTPLMEAVAL
                     KYPFYMNALKRKINENWSPPGAGFAQTRATLVVFTIQRDGSVRGMNVEESSGDAFYDQ
                     AAVRSVLRASPFPPLPPGYTEESMKIFFSFSLDPDRPS"
     gene            1262..2509
                     /gene="tolB"
                     /locus_tag="AC16BC_03755"
     CDS             1262..2509
                     /gene="tolB"
                     /locus_tag="AC16BC_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR3240763.1"
                     /translation="MLCAWCAVAFGSDVYLGLSRSAGAKIPVAVLGVQAAPDLADEAS
                     ALRSVIESDLRRSLLFTVVTLPVEDTAKVPDVARIKEAGAAGADVVIWGRLSRDRDGR
                     LVWEGKGYDGGSGAVVVGKLLRVPEKYLRSMAHSFSDALVAGFTGEKGIAQTRIAFVS
                     DRSGKKELYLMDYDGANVIPITRDRSIALAPRWSPDGKTITFVSYRDGTPALHSYLPV
                     SGRRWPIVALPGTTMSPAWSPTGDRLALSSSQAGDPDIYLADAEGKNLRRLTFGLGAD
                     LSPSWSPNGKQIAFNSDRGGSAQIYLMNADGSDQRRLTFDGTYNTTPAFSPKGDWIAH
                     TCRVEGRDQICLISPDGANRVQVTNSPGDNDSPSWAPGGRHIVFASNRQGDTHLYVIG
                     VDGTGEEALTSGAGNDQTPGWSP"
     gene            2538..2876
                     /locus_tag="AC16BC_03760"
     CDS             2538..2876
                     /locus_tag="AC16BC_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240764.1"
                     /translation="MSRTRRDLDLKLTKKWLSSNAVREGLLTSLVLALREAGEPGDME
                     GLVDRAAQEEFKRYGADEEYAPPATVRNVLAALETRFGVDRLPADVLARHREVCAALL
                     SKVDQQSGYR"
     gene            complement(3160..3624)
                     /locus_tag="AC16BC_03765"
     CDS             complement(3160..3624)
                     /locus_tag="AC16BC_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240765.1"
                     /translation="MTAMPYVKLLCIAALAAIGSFMAADGVSAAPNDLVFVASSETPV
                     QSLKTDEILNIYLGKQRVANGFPVSAIDHSETETIKRTFLERVMRMTHAQYREQLMRR
                     RFKEGAVTPKFVTSADEALNVARETPGAIAYVYESDAGTLRGLKVLATIPAK"
     gene            complement(3636..4721)
                     /locus_tag="AC16BC_03770"
     CDS             complement(3636..4721)
                     /locus_tag="AC16BC_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240766.1"
                     /translation="MGNRTPLWSICFAILAAFILSTSRPAAAFELHGFADVSYQHDIH
                     DNVAPDENNGAFVIGPLDFYVAESLNPRVDVLAEFAIESGVVDLERLQIGYLFSDLLK
                     VYAGRFHTSLGYWNTAYHHGTFLYTTIERPFFLQFEDDAGILPTHSVGLLLTGRQFLP
                     SGELSYSAVVSNGSSVTVEDGANILDPNNESDPNHNKAVGLRVAFAPSGLSGWAVGAS
                     AMTNHVINSTPLVGPPTDIDVTQTIVGADVTHTQGPWELLAEYFLLRDKDKVGADTAT
                     NHMYYLQVGREFRGTVTPYARHEQMSFDENDPYLNVLGAADKRTNTMGVRVRLGEQSV
                     LKFEGRFITDNGIDSHQEYGAQWAFAF"
     gene            complement(4778..6064)
                     /locus_tag="AC16BC_03775"
     CDS             complement(4778..6064)
                     /locus_tag="AC16BC_03775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR3240767.1"
                     /translation="MTTSGERLSLTVDKVIHGGRGMGRSAGCPVFVGGTLPGERIEAR
                     VTARRKGYLEAAVERVLAPSTDRIEAPCAAYPACGGCQLQHAGYSAQLALKRALVQET
                     LQRVGGVALDVPPLIPSPEPFGYRLRAQIKIDRRSGPPVLGFYAAGSHRVVPAPTCLV
                     LHPRLQEALSILQGFAARDDPRLNGVREVELQTTSCADDGLIVLHGDSFRPASLRTLA
                     RDLRASAPVRGLVAYSRRGRRVEGADWLDETVDGVRCRVSDRTFLQINAGVNAALIAT
                     VAAWAALTGRERVVDLYAGFGNLSLPLARHGVVTAVESSPSAVADARWNARASGRPVH
                     VVGQSVDEWSPKPDDRRPDLVVMDPPRSGLTRAALSRVLMLAAPRLLYVSCEPATLAR
                     DVKRLVQAGYAVRRIVGFDMFPQTAHAETVAELSKI"
     gene            complement(6061..>7069)
                     /locus_tag="AC16BC_03780"
     CDS             complement(6061..>7069)
                     /locus_tag="AC16BC_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3240768.1"
                     /translation="NRRLRATITRRLTLVGDSPLMTRLKSEIATAAASHSRVLISGEN
                     GTGKEVVARLIHAQSPRASGPFVDVNCAAIPEALIESELFGHERGAFTGATAMKRGKF
                     EEADGGTLFLDEVADMSLATQAKVLRVLQEQRFSRVGGTRPIDVDVRVISASNKDLRE
                     AIGRGAFREDLFYRLNVIPLHVPPLRDRRDDIPALVDHFLREIAADQGLKPKTIHPQA
                     LDALMQYRWPGNVRELRNTIERLLIMVAGAAIQPEDVAPSLSGAARSGGAASAAPDGL
                     TLLKDARAAFERDYIARSLRVHDFNVSKTAEALGLERSHLHRKIKMLGIEVRPDAGGE
                     A"
BASE COUNT         1176 a         2230 c         2374 g         1289 t
ORIGIN      
        1 tcgctccaga atttttccgg gttcttctgc gcgagcttgg cgagtttgtc gtattccttg
       61 atgttcttga tatgggcgga gcgactgaac gcggctttgg gtttgaacac gcgccgttcg
      121 ttcagcacgg actcaatctc gggctgggac atcgtggagt ctccttcgag gtcagtgacg
      181 tctgcgaggc actggacgct ccgattcgcg cggatcacgc ggccggagcg ttgaggcgag
      241 cattgtaggg aacgctcgac tgacttgtca atcagtcgtt aggagcctgt ccatgaaagg
      301 ccatctgcgg cgttgtcggt cgaccttgcg tgctcacgta cgagccagta cgctccgcgc
      361 gcaaggctct ccctccgcct ggcatctggc cttccctgga ctggctccta aaatgggtta
      421 ctcgggacag gctatagccg cgacgtggtg gcccgccgaa ccagtctcat gacgtccacg
      481 ctgtcccgcc gatcatcctt gcgtccgatg gtcattctct cgggcgcgct ccacgcggtg
      541 tttgccgtgc ttctcgtgtg ggggccgtcg tggatcccga ggcgccccgc gatgccggtg
      601 tacgaggtgc agttggtcag cgtcccggtc gaagcgccgc ggcctcctcg tcaaccgccg
      661 cccccgcgcg tcgagccgaa accgccgcct ccgccgccca agcccgtcaa ggcgaaaccg
      721 gtgaagaagc ccgtgcaggc cgcggtggaa cccgcgccga ccaaggcgca tccggcgccg
      781 gcgccggtcc ctgagcccgc gaagacgccg ccccagcccg agcggcagcc cgatccgccc
      841 gcgccggaac cccaggccaa ggccgcggaa ccgaccgagc ccggcattca gttgatgacg
      901 ccgttgatgg aagcggtcgc gctgaagtat ccgttctaca tgaacgcgct caagcgcaag
      961 atcaatgaga actggtcgcc gcccggcgcg ggattcgccc aaacccgagc caccttggtg
     1021 gtgtttacca ttcaacgcga tggcagcgtc cgcggcatga atgtcgagga gagctcaggg
     1081 gacgcgtttt atgatcaggc ggcggtccgc tccgtgttgc gggcgagccc gtttccgcct
     1141 cttccacccg gatacaccga ggagagcatg aaaatctttt ttagcttttc gctcgacccg
     1201 gatcgaccgt cgtgaccgca tgagcgtaaa acggtggatg atctggtcat cgatgccggc
     1261 gatgctgtgc gcctggtgcg cggtcgcctt cggctcggac gtctatctgg gcttgtcgcg
     1321 atccgcgggc gcgaaaattc cggtggccgt gctcggcgtg caggctgctc cggatttggc
     1381 cgacgaagcc tcggcgctgc gatcggtgat cgaatcggac ctgcgtcggt cgttgctgtt
     1441 tacggtcgtg acgttgccgg ttgaagacac cgcgaaggtg ccggatgtcg cgcggatcaa
     1501 ggaagcgggc gcggcaggcg cggatgtggt gatctggggg cgcttgtcgc gtgatcgcga
     1561 cggacgccta gtgtgggagg gcaaggggta cgatgggggc agcggggccg tcgttgtggg
     1621 gaagcttctt cgcgtgcccg agaagtatct gcgctcgatg gcgcacagct tttcggatgc
     1681 cctggtcgcg gggttcacgg gagaaaaagg gattgcccag acgcggatcg cctttgtctc
     1741 tgaccggtct gggaaaaaag agttgtatct gatggactat gacggcgcga acgtgattcc
     1801 gattacgcgc gaccgcagca tcgcgctcgc gccccggtgg agtcctgacg ggaaaacgat
     1861 caccttcgtg tcgtaccggg atgggacccc ggctcttcat tcgtaccttc cggttagcgg
     1921 tcgccggtgg ccgatcgtcg cgttgcccgg caccaccatg tcgcccgcgt ggtcgccgac
     1981 cggcgatcgg ctcgccttgt cgtcgagcca ggcgggcgat cccgacattt acctggcgga
     2041 tgccgagggc aagaatctcc gccggctgac gttcgggctt ggcgcggacc tctcgccgtc
     2101 gtggtcgccc aacggaaaac agatcgcgtt caactcggac cgcggcgggt cggcgcagat
     2161 ctatctgatg aacgccgacg gcagcgatca acgccggctc acgttcgacg gaacgtacaa
     2221 caccacgccg gccttctcgc ccaagggcga ctggatcgcc cacacctgtc gcgtcgaggg
     2281 gcgggatcag atctgtctga tcagtccgga cggcgccaac cgggtgcagg tgaccaactc
     2341 gcccggagac aacgacagtc cgtcgtgggc gcccggcggc cggcacatcg tgttcgcctc
     2401 gaaccggcag ggcgataccc atctctacgt gatcggcgtg gatgggaccg gggaggaggc
     2461 gctgacctcc ggagccggca acgatcagac gccggggtgg tcgccgtagt cgggccgacg
     2521 ggaaggagcg cagaccagtg agtcgcacgc gacgggatct ggacctcaaa ttgaccaaga
     2581 agtggctgtc gagcaacgcg gtgcgggagg gcctgctgac cagtctcgtg ctcgcattgc
     2641 gggaggcggg ggagcccggc gacatggagg ggctggtgga ccgcgcggcg caggaggaat
     2701 tcaagcgcta cggcgcggac gaagagtacg cgccgcccgc cacggtgcgc aacgtgctgg
     2761 ccgcgctgga aacgcgtttc ggcgtggatc gactgcctgc ggatgtcctg gcgcggcacc
     2821 gcgaggtttg cgcggcgctg ctctcgaaag tggatcagca gagcgggtac cgctaagagc
     2881 ccgtccagga agaaccatct gcggcgttgc cgctgcgcat ccggtgctcg cggcgccttg
     2941 catctggtcc ttcctgaacg ggctccaccc tgacattatt catgcgggtg agcagcccgc
     3001 ttgccccccc ttttcaaagg ggggaggttc atgccgcgct ggtgggccgc acctatgacc
     3061 acctgtctca aatcctccgt cccccccctt tgttaacggg gggacggagg atttccttgc
     3121 gcttcggaac gagtggtgtc ttccttcgtg accggttcgt tatttggcgg ggatcgtggc
     3181 caggactttc aagccccgca aggttccggc gtccgactcg tagacgtacg cgatcgcgcc
     3241 cggagtctcg cgcgcgacat tcagggcttc gtccgcgctc gtcacgaact tcggggtgac
     3301 cgcgccttcc ttaaaccggc gtcgcatcaa ctgttcccgg tattgggcgt gcgtcatgcg
     3361 catgacgcgt tcgaggaagg tccgtttgat ggtttcggtt tcgctgtgat cgatggcgga
     3421 cacgggaaac ccattggcca cgcgctgttt tcccaggtag atgttcagga tctcgtccgt
     3481 cttgagggat tgaacggggg tttccgacga ggccacgaac accaggtcgt tgggagcggc
     3541 cgagacgccg tccgccgcca tgaacgaacc aatcgccgcg agcgcggcga tgcagagcaa
     3601 cttcacgtaa ggcattgctg tcatgctggc ggctcctaaa acgcgaacgc ccattgggcg
     3661 ccgtattctt gatgggaatc gatcccgttg tcggtgatga accgaccctc gaacttcaga
     3721 accgattgct ctcccaatct gacccggacg cccatggtgt tcgtgcgttt gtctgccgct
     3781 ccgagcacgt tcagatacgg gtcgttctcg tcaaacgaca tttgttcgtg gcgcgcatat
     3841 ggggtgaccg tgccgcggaa ctcgcgcccg acctggaggt aatacatgtg gttggtcgcc
     3901 gtgtccgcgc ccaccttgtc tttgtcgcgc aggagaaagt attcagccag cagttcccac
     3961 ggcccctgag tgtgcgtgac gtcggccccg acgatggtct gggtcacgtc gatgtcggtc
     4021 ggcggtccga ccagcggcgt cgaattgatc acgtggttgg tcattgcgct ggcacccacg
     4081 gcccaaccgg acaagccgga gggcgcaaag gccacccgca gcccgaccgc cttgttgtgg
     4141 ttcgggtctg actcgttgtt cgggtcgaga atattcgcgc cgtcttcgac cgtgaccgat
     4201 gagccgttgc tgaccacggc gctgtaggag agttccccgc tgggaaggaa ttggcgaccg
     4261 gtcaacaata acccgacgga gtgcgtcgga aggattcctg cgtcgtcttc gaattggagg
     4321 aagaacggtc gttcgatcgt ggtgtaaaga aatgtgccat ggtggtatgc ggtgttccaa
     4381 taccccagcg acgtatggaa acgcccggcg tagaccttga ggagatccga gaacaagtat
     4441 ccgatctgga gacgttcgag gtccaccacg cccgattcga tggcaaattc cgcgagcacg
     4501 tccacccgcg ggttgagcga ctcggcgacg tagaagtcga gcggtccgat cacgaacgcg
     4561 ccgttgtttt cgtccggagc aacgttgtcg tggatgtcgt gttgatacga gacgtcggcg
     4621 aatccgtgaa gctcgaaggc ggctgccgga cggctcgtac tgaggatgaa cgccgccagg
     4681 atcgcgaaac agatactcca gagtggtgtc cggtttccca ttgttggctc cctcctgact
     4741 gtacgctgtt tgtgtatagt ccacgagggg aatgctgtca aattttagat agctctgcca
     4801 ccgtctcagc gtgcgcggtc tggggaaaca tgtcgaagcc gacgatgcgg cgtactgcgt
     4861 agccggcttg aaccaagcgc ttgacgtccc gcgccaacgt ggcgggctcg cacgacacgt
     4921 acaggaggcg cggagccgcg agcatcaaca cgcgggacaa cgcggcgcgg gtcaggccgg
     4981 atcgcggggg atccatgacc acgaggtccg ggcgacgatc atcgggtttc ggcgaccact
     5041 cgtcaacgga ttgtccgacc acgtgaaccg gacggccact cgctcgcgcg ttccatcgcg
     5101 cgtcggcgac ggcgctcggg gatgattcca cagcggtcac gacgccgtga cgcgcgagcg
     5161 ggaggctcaa gttgccgaag cccgcgtaca aatccaccac gcgttcgcgg cccgtcagcg
     5221 ccgcccacgc cgccacggtg gcgatcaacg cggcgttgac gccggcgttg atctgcagga
     5281 acgtgcggtc gctcacccgg caacgcacgc cgtcgaccgt ctcgtcgagc caatccgcgc
     5341 cctccacgcg gcgtccgcgg cgggagtacg cgacgaggcc gcgcaccggc gccgaagccc
     5401 gaaggtcgcg tgccagcgtc ctcaacgacg ccgggcggaa tgaatcaccg tgcagcacga
     5461 tcaggccgtc gtcggcgcac gacgtggtct ggagttcgac ttcgcgcacg ccgttgagac
     5521 gcggatcgtc ccgcgccgcg aatccttgga ggatgctcag ggcttcctga agacgcgggt
     5581 ggaggacgag gcaggtgggc gcgggcacca cgcggtggct gcccgcggcg tagaatccga
     5641 gcacgggtgg tccggaacgt cggtcgatct tgatctgggc gcgcaggcga tacccgaacg
     5701 gttcgggtga tggaatgagc gggggcacgt cgagcgccac gccgccgacc cgctgaaggg
     5761 tttcctggac cagggcgcgt ttgagtgcca actgggcgga ataccccgcg tgctgcaact
     5821 ggcacccccc gcacgcggga taggccgcgc acggcgcctc gatgcggtcc gttgacggcg
     5881 cgagcacgcg ctcgaccgcg gcctcaaggt atcccttgcg ccgcgcggtg acgcgcgcct
     5941 cgatccgctc gcccgggagc gtgccgccca cgaacaccgg gcatccggcg gagcgcccca
     6001 tcccccgtcc cccgtgaatc accttgtcga cggtgaggga caggcgctct ccggacgtgg
     6061 tcacgcctct ccgccggcat ccggccgcac ctcgatcccc agcatcttga tcttgcgatg
     6121 caggtggctg cgttccagcc ccaaggcttc ggcggtcttg ctgacattga agtcgtgaac
     6181 gcggagcgat cgggcaatgt agtcgcgttc gaacgcggcc cgcgcgtcct tgagcagcgt
     6241 gaggccgtcg ggagccgccg acgcggcccc tccggatcgc gccgccccgc tcaacgaggg
     6301 cgcgacgtct tccggttgga tggcggcccc cgcgaccatg atcagcagac gctcgatcgt
     6361 gttgcgcaac tcgcggacat tgccgggcca ccggtactgc atcaaggcgt cgagggcctg
     6421 cgggtggatg gttttgggtt tgagaccctg atcggccgcg atctcccgga gaaagtggtc
     6481 taccaacgca gggatgtcgt cgcgccggtc ccgcaacggc ggcacgtgaa gcggaatcac
     6541 gttgaggcga tagaacagat cctcgcgaaa cgcgcctcgc ccgatggcct cccgcagatc
     6601 cttatttgag gcgctgatga cccgcacgtc cacgtcgatc ggccgagttc cgccgactct
     6661 ggagaaccgc tgctcctgca gcacgcggag caccttggcc tgggtggcga ggctcatgtc
     6721 ggcgacttca tcgagaaaca acgtgccgcc gtcggcttcc tcgaatttgc cccgcttcat
     6781 cgcggtggcg ccggtgaacg cgccgcgctc gtggccgaac agctcgcttt caatgagcgc
     6841 ctccggaatc gccgcgcagt tcacgtccac gaacgggccc gaggcccgcg ggctctgggc
     6901 gtggatgagg cgcgcgacca cttccttgcc ggtgccgttt tccccggaga tcaagacccg
     6961 gctgtgtgag gcggcggccg tcgcgatctc ggacttgagt cgcgtcatca acgggctgtc
     7021 gccgaccagc gtcagccgac gggtgatcgt ggcccgcagg cgccgattt
//
LOCUS       DBNGTT010000165         1941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_801101, whole genome shotgun sequence.
ACCESSION   DBNGTT010000165 DBNGTT010000000
VERSION     DBNGTT010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_801101"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC16BC_03785"
     CDS             complement(<1..365)
                     /locus_tag="AC16BC_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240769.1"
                     /translation="MTTPVHTPYSQKKLPLLRTEELMLNMGPQHPSTHGVLKVVLELE
                     GERIMKSTPVMGFLHRGVEKLAEDGTYHQFIPHTDRLDYVCAMYNNFAYVRAVEKLMG
                     ITVPPRSEYLRTIVAEVQR"
     gene            complement(369..887)
                     /locus_tag="AC16BC_03790"
     CDS             complement(369..887)
                     /locus_tag="AC16BC_03790"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3240770.1"
                     /translation="MHPTADQIKSKFPDAFVRATEFRGDLAVEVTPGSVLDVARFLRE
                     DPGMDYDYIVHVSSVDWLGEPVRFEVVWEVYSVRRRHRLRLKTRVPESEPVVESLTPF
                     WKGCDFMEREVYDMMGIRFLHHPDLRRILMPDDYTEGFPLRKDFPMVGKGWRDTFDFL
                     DAPAQPVPPGSS"
     gene            complement(891..1421)
                     /locus_tag="AC16BC_03795"
     CDS             complement(891..1421)
                     /locus_tag="AC16BC_03795"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3240771.1"
                     /translation="MGLINISPRSGDGKTGDVGFVTLKLEQAVNWARKGSLWPMTFGL
                     ACCAIEMIAAVSSRYDMDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKVYDQMPE
                     PRYVIAMGSCATSGNIYNSYPVVQGVDRFVPVDIYVPGCPPTPEALFDGILRLQEKIM
                     QQRVFVQRESFGAIKT"
     gene            complement(1412..1813)
                     /locus_tag="AC16BC_03800"
     CDS             complement(1412..1813)
                     /locus_tag="AC16BC_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3240772.1"
                     /translation="MVFDARMGLDTVLSAYLANYGPVVIFMAIAAAFGAGTLVFSYVI
                     QPRIYSSEKLSAYECGSEPISDARMPFPVRYYIIAMLFVIFDVELIFLYPWAVAFDKI
                     GLFGLIEMIIFILLFVAAYAYAWRKGALEWD"
BASE COUNT          403 a          607 c          588 g          343 t
ORIGIN      
        1 atgcgctgca cttccgccac gatcgtgcgc aggtactcgg atcgcggggg caccgtgatg
       61 cccatgagct tttccacggc ccggacgtac gcgaaattgt tgtacatcgc gcacacgtaa
      121 tcgagccggt ccgtgtgggg aatgaactgg tggtacgtgc cgtcctcggc gagtttctcc
      181 accccgcggt gcagaaaacc catgaccggc gtggatttca tgatccgctc gccttccagc
      241 tccaacacca ctttcaacac gccgtgcgtg ctggggtgct gcggccccat gttgagcatc
      301 aactcctcgg tgcgcaggag cggcagtttc ttttgcgaat acggggtgtg aaccggcgtg
      361 gtcatggctt acgaggaccc gggcggaacg ggctgcgcgg gcgcgtcgag gaaatcgaac
      421 gtgtcgcgcc agcccttgcc caccatgggg aagtctttgc gcagggggaa accttcggtg
      481 tagtcgtccg gcatcaggat gcgccgcagg tcgggatggt gcagaaaccg gatgcccatc
      541 atgtcgtaga cctcgcgctc catgaagtcg caccctttcc agaacggggt gagcgactcc
      601 accaccggct cggactccgg cacgcgcgtc ttgaggcgca ggcggtggcg acggcgcacg
      661 gagtacacct cccagacgac ctcgaaccgc accggctcgc ccagccaatc caccgagctc
      721 acgtgcacga tgtagtcgta gtccatgccc gggtcttcgc gcaagaaccg cgcgacgtcc
      781 agcaccgacc cgggtgtcac ctccaccgcc aaatcccccc gaaactccgt ggcccgcaca
      841 aacgcgtcgg gaaacttgga tttgatctga tcagcagtcg gatgcatggc tcaggtctta
      901 atcgcgccga aggactcgcg ttgaacgaag actctttgtt gcatgatttt ctcctgcagc
      961 cgcagaatgc cgtcgaacaa tgcttccggc gtcggcgggc agccgggtac gtagatgtcg
     1021 accggcacga accgatccac gccttggaca accgggtagc tgttgtaaat gttgccggac
     1081 gtggcgcatg aacccatggc gatcacatac cgcggctccg gcatctggtc gtagaccttg
     1141 cggatgaccg gcgccatgcg acggcagacc gtgcccgcca cgatcatcag gtcggactgc
     1201 cgcggcgacg cccggaacac gcccgcgccg tagcggtcca tgtcatagcg cgacgatacc
     1261 gccgcgatca tttcgatcgc gcaacacgcc agtccgaatg tcatgggcca cagcgagccc
     1321 ttgcgcgccc agttcacggc ctgttcgagc ttcaacgtga caaaccccac gtccccggtc
     1381 ttgccgtcgc ccgagcgcgg gctgatattg atcaatccca ttcgagcgct cccttgcgcc
     1441 acgcataggc gtacgcggcc acgaacaaga ggatgaaaat gatcatctcg atcaagccga
     1501 acagcccgat cttgtcgaac gccacggccc agggatagag aaagatcagt tccacgtcga
     1561 agatcacgaa caacatggcg atgatgtagt agcggaccgg gaacggcatg cgcgcgtcgg
     1621 agatcggctc gctgccgcac tcatacgcgg agagcttttc ggaggaatag atccgcggct
     1681 ggatgacgta actgaagacc agggtcccgg cgccaaaagc cgccgcaatc gccatgaaaa
     1741 tgacgaccgg accatagttt gccaggtatg cggagagtac cgtgtcgagc cccatacgcg
     1801 cgtcaaagac cattgatttt tctactggag cgcaggttgt agcacgccct attttaggca
     1861 gtcaagcggc taaaggacct aaggacctct ttggggcgaa gggcctagtg tcagaacggc
     1921 ggagaaacag agacggggcg a
//
LOCUS       DBNGTT010000166         3040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3355779, whole genome shotgun sequence.
ACCESSION   DBNGTT010000166 DBNGTT010000000
VERSION     DBNGTT010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3355779"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="AC16BC_03805"
     CDS             <1..1060
                     /locus_tag="AC16BC_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR3240773.1"
                     /translation="VDTLPQVLEFLEGRQPLVRATAGSVEASVPIEADDFGDVKGQEH
                     AKRALEVAAAGGHNVLMVGPPGSGKTMLARALAAILPKMTVDEALECTKIHSVMGLLA
                     PETPLLSARPFQAPHHTISDAGLIGGGQIPKPGEVSLAHHGVLFLDELPEFRRNVLEV
                     LRQPLEQRRVTIARSSASVTYPAGFMLVAAMNPCPCGHFGDRTHQCTCTPVQIQRYRA
                     RVSGPLLDRIDLHVDVPAVPFGDLSSDHAREDSSRVRARVMKAREIQQTRYAGEGLYC
                     NAQLRPRLLKRHCEINADARRLIEQAMTKLGLSARAYHRILKVARTVADLDGSGPVLP
                     GHVAEAIQYRNWDRRVVH"
     gene            1074..>3040
                     /locus_tag="AC16BC_03810"
     CDS             1074..>3040
                     /locus_tag="AC16BC_03810"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR3240774.1"
                     /translation="MDWERMNPGWTGRSWRRLAPWLMAAGVLAAVSAVAGGAAVWALS
                     RNLPDIGTLRSYQPSLVTRVYADDNRQIGQFFVEKRVLTPLARIPKSLINAVIAVEDS
                     RFYQHEGLDVLRIVKALVVDVVSMQMREGASTITQQLARSLFLTPEKSLKRKAKEMLL
                     ALKIERMLSKDEILEMYLNQIYFGHGSYGVQAAARTYFAKDVGDLTLAEMAFVAGLPK
                     APTNYSPYFNPERAKQRQGVVLKRMLDEGFLTDAEFRSAYRQDLYFAKYQPQEQIAPY
                     FLEYLRQQLTATYGEELVYKGGLNVYTTLNVEMQTAAVDALRDGLRAIDKRQGFRGAL
                     AQGQEPPDAAASNANVSPLKPGDIVEGVVTKVGPQHAAVAVAGGSGVLAMEDAVWARK
                     RLLPPDFRTAEFRAGAPLNKILAAGDRILVRVKRRDDRGGFSFALDQEPIVEGAVIAL
                     DPRTGAIRAMVGGYDFKRSEFNRAVSARRQPGSAFKPFIYAAAIEAGWTPASILVDSP
                     VIYDDPVLQRVWKPTNYEARFYGPITMRDALIHSRNVATVKLLDQIGIQPAIDFAHRV
                     GITSPLARDLSLALGSSSLGLLEVTSAMGVFAAEGRRVEPAGLRSVTDHAGNVLAYYE
                     PAPVDVVSRETAYLITNMLEDVVQSGTGMRAR"
BASE COUNT          533 a          900 c         1074 g          533 t
ORIGIN      
        1 ggtcgacacc ctcccgcagg tgttggagtt tctcgaaggc cgacagccgt tggttcgcgc
       61 gacggccgga tcggtggaag cctcggtgcc gatcgaagcg gacgacttcg gcgacgtgaa
      121 gggccaggaa cacgccaagc gcgcgctcga agtggcggcc gccggcgggc acaacgtgtt
      181 gatggtcggg cctccggggt ccgggaagac catgttggcc cgggcgttgg cggcgattct
      241 gccgaagatg accgtggacg aagcgttgga gtgcaccaag atccacagcg tcatggggtt
      301 gcttgcgccg gagacgcccc tgctgtcggc gcgaccgttc caggcgcccc accacacgat
      361 ctcggacgcg ggactgatcg gcggcggaca gattccgaaa cccggcgagg tcagcctcgc
      421 ccatcacggc gtcctgtttt tggacgaatt gccggagttt cggcggaacg tgttggaagt
      481 cctgaggcag ccgctggaac agcggcgggt gacgatcgcg cggtcctcgg cgtcggtgac
      541 gtatcccgcg ggttttatgc tcgtggccgc gatgaacccg tgtccgtgcg gacatttcgg
      601 ggatcgcacg catcagtgca cgtgtacgcc ggttcagatc caacgctacc gcgcgcgggt
      661 ctcgggaccg ttgctcgatc ggatcgatct gcacgtcgac gtgccggccg tgccgtttgg
      721 agacctgagc agtgatcacg cgcgcgagga ctcgtcgcgg gttcgcgcgc gggtcatgaa
      781 ggcgcgcgag attcagcaga cccggtacgc cggggagggg ttatactgca acgcacagtt
      841 gcgaccgcgc ctgctgaaac gccactgcga gatcaacgcg gacgctcggc gactgatcga
      901 gcaggcgatg accaagctcg gcctgtcggc gcgggcgtac catcggatct tgaaggtggc
      961 gcgcaccgtg gccgacctcg atgggtcggg cccggtgttg cccggtcacg tggcggaagc
     1021 cattcagtat cgcaactggg accgacgggt ggtgcattga cggtgagacg catgtggact
     1081 gggaacgtat gaatccgggt tggacgggac gatcgtggcg ccggctcgcg ccgtggctga
     1141 tggccgccgg agtgctggcg gcggtgtcgg cggtggcggg cggggccgcg gtctgggccc
     1201 tgtcgcgcaa tctcccggat atcgggacac tgcggtcgta tcagccgagc ctcgtcaccc
     1261 gggtgtacgc ggacgacaat cgccaaatcg ggcaattctt cgtcgaaaaa cgcgtcctga
     1321 ctcccctggc gcggattccg aagtcgctca tcaacgcggt gatcgccgtc gaggactcgc
     1381 gattctacca gcacgagggg ctggacgtgc tgcgcatcgt gaaagccttg gtggtggacg
     1441 tcgtgtcgat gcagatgcgc gagggcgcga gcacgatcac gcagcagctg gctcggtcgc
     1501 tcttcttaac cccagaaaag agtctgaagc gcaaggcgaa agaaatgctt ctcgccctca
     1561 agatcgagcg gatgctctcg aaggacgaga ttctggaaat gtatctcaac cagatctact
     1621 tcggacacgg ttcctacgga gtgcaggcgg ccgcgcgcac gtatttcgcc aaggacgttg
     1681 gagacctcac gctggccgaa atggcctttg tcgcgggact gccgaaggcc ccgaccaact
     1741 actcgcccta cttcaatccg gaacgggcca agcagcgtca aggcgtggtc ctcaaacgaa
     1801 tgctggacga agggtttctg actgacgccg aatttcgctc cgcgtaccga caggacttgt
     1861 atttcgccaa atatcagccg caggaacaga ttgccccgta cttcttggaa taccttcgac
     1921 aacaactgac cgcaacctat ggggaagagc tcgtctacaa gggcggcctc aacgtgtaca
     1981 ccacgctcaa cgtcgagatg cagacggcgg cggtggacgc gttgcgggac ggcctgcggg
     2041 cgatcgacaa gcggcaaggc tttcgcgggg cgctggctca gggccaggag ccccccgacg
     2101 cggcggcgtc gaacgccaat gtctcgccgc tgaaaccggg cgacatcgtg gaaggcgtgg
     2161 tcaccaaggt cgggccgcag cacgccgcgg tcgcggtggc cggcggatcg ggagtgttgg
     2221 ccatggagga cgcggtctgg gcccgcaagc ggttgctgcc cccggatttc cggaccgcgg
     2281 agttccgggc cggagcgccg ctcaataaaa tcctggccgc gggcgatcgc atcctggtgc
     2341 gagtgaagcg gcgcgatgat cgaggcgggt tctcgtttgc gctcgaccag gaaccgatcg
     2401 tggaaggcgc ggtgatcgcg ctggacccgc ggaccggagc catccgcgcg atggtcggcg
     2461 ggtatgactt caagcgcagc gaattcaatc gggccgtgtc ggcgagacga cagccgggct
     2521 cggcgttcaa gccgtttatt tacgcggccg cgatcgaggc cgggtggacg ccggcgtcca
     2581 ttctggtgga ttcgcccgtg atctacgacg acccggtgct gcagcgggtg tggaagccga
     2641 cgaattacga agcccgtttt tatggcccca ttacgatgcg ggacgcgttg attcattccc
     2701 gcaacgtcgc gacggtcaag ttgttggatc agatcggcat ccagccggcg atcgattttg
     2761 cacaccgagt cggtattacg agcccgttgg ccagggatct gtcgctggcg ctggggtcat
     2821 cgagtctggg gttgctggag gtgacctcgg ccatgggcgt gttcgcggcg gaggggcggc
     2881 gggtcgagcc tgccgggctg cggtccgtga cggaccacgc tggaaacgtg ctcgcctatt
     2941 acgaacccgc gccggtcgac gtggtctcgc gggagaccgc atatctgatc acgaacatgt
     3001 tggaggacgt ggtgcagtcc ggaaccggga tgcgggcccg
//
LOCUS       DBNGTT010000167         2571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1951455, whole genome shotgun sequence.
ACCESSION   DBNGTT010000167 DBNGTT010000000
VERSION     DBNGTT010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1951455"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="AC16BC_03815"
     CDS             <1..1171
                     /locus_tag="AC16BC_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3240775.1"
                     /translation="EVVSLLDMLVLPLKERGEKTYSAPFATHLYAKETIERPDGTQTR
                     DFPRLRYGGAHLADPFADRGRDIATTALAGAFQGLLLWLPIVGLVVTWQARRNRSAWG
                     ATLGDVTTGRTDTPWRVILATLGVLLVFGFVIAHWAVKYHVLGTDKVGTDVLYLSLKS
                     IRTGIVIGTLTTLIMLPFAVLLGIMAGYLKGWADDVIQYVYTTLSSIPAVLLIAAAIL
                     TLQAYMALHPDIFDTAAIRADTRLLFLCAILGVTSWTGLCRLLRGETLKLREAEYIQA
                     ARAYGAGSLTIMLRHILPNVMHIVLITVVLDFSGLVLAEAVLSYVGVGVDPTTISWGT
                     MINSARLEMAREPMVWWSLAAAFTFMFSLVLAANLFADVVRDAMDPRASRDRTTA"
     gene            1245..1574
                     /locus_tag="AC16BC_03820"
     CDS             1245..1574
                     /locus_tag="AC16BC_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GroES family chaperonin"
                     /protein_id="HIR3240776.1"
                     /translation="MKIRPLHDWAVIKQSSGDERTAGGLYIPDSAKEKPQQGEVIAIG
                     EGRWKSKDEDLRSTKKKGADEKTFVKTTLKPGDKVLYERYAGSKVTINNEEFVMVREE
                     NVLGILA"
     gene            complement(1635..2216)
                     /locus_tag="AC16BC_03825"
     CDS             complement(1635..2216)
                     /locus_tag="AC16BC_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR3240777.1"
                     /translation="MKDGSGRFFVLQDALRGRIDALQAKINAGMEFALDHLFEHQIDH
                     YFRHEEALEELEALCQRVTADFDSLDSFDALKRFEARFVILEDRCEEVDAALRQRPMR
                     RRQRINLSDFFRQSQGGNGRAEASHPEVSSLTEAYAILGVEPDTRPALITAMFRKLVK
                     ELHPDANNGDRSHEPRLRKLMAAYQLIKAQVTK"
     gene            complement(2370..>2571)
                     /locus_tag="AC16BC_03830"
     CDS             complement(2370..>2571)
                     /locus_tag="AC16BC_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240778.1"
                     /translation="LILLESTLDRRTIVAPEDYDEIELPPLVIPKRDAPECPFCGEKM
                     IPVDGDYICLDCNGGSYGPETG"
BASE COUNT          482 a          820 c          765 g          504 t
ORIGIN      
        1 cgaagtggtg agcttgctcg acatgctggt tctcccgctc aaggagcgcg gggagaagac
       61 ctactccgcc ccgttcgcca cgcacttgta cgccaaggaa accattgaac ggcccgacgg
      121 cacgcaaacg cgagactttc cccgcctgcg ttacggcggc gcgcacctgg ccgatccctt
      181 cgcggatcgc gggcgggaca tcgcgaccac ggcgctcgcg ggcgcgttcc aaggcctgct
      241 cctctggctg ccgatcgtcg gactcgtcgt gacgtggcag gcgcggcgca atcggagcgc
      301 ctggggcgcc actctcggcg acgtgaccac gggccgcacg gacacgccgt ggcgtgtgat
      361 cctcgcgacc ctcggcgtcc tgctcgtgtt cggattcgtg atcgcccact gggccgtgaa
      421 gtaccacgtg ctcggcaccg acaaggtggg caccgacgtg ttgtacctgt cgctcaagag
      481 catccgcacc ggcatcgtga tcggcactct gaccacgctc atcatgctgc cgttcgcggt
      541 cctgctcggg atcatggccg gctatctgaa gggctgggcg gacgacgtga tccaatacgt
      601 gtacaccacg ttgagttcga ttcccgcggt gttgctcatc gccgcggcca tcctgacgct
      661 gcaggcctac atggcgctcc atcccgacat cttcgacacc gcggcgatcc gcgccgatac
      721 gcgtctgttg ttcctgtgcg ccatcctcgg agtgacgagt tggaccggct tgtgccggtt
      781 gctgcgcggc gaaacgctca aattgcgcga agccgaatac atccaggccg cgcgggcgta
      841 tggcgcggga tccctgacga ttatgttgcg acatattctt ccgaacgtca tgcatatcgt
      901 gctgatcacc gtggttctgg atttttccgg tctggtgctg gccgaggccg tgctttcgta
      961 cgtgggcgtg ggcgtggatc ctaccaccat cagttggggc accatgatca acagcgcccg
     1021 cctggaaatg gcgcgcgagc ccatggtctg gtggtcgctt gccgcggcgt ttactttcat
     1081 gttcagcctg gtcctggccg cgaatctctt cgccgacgtg gtgcgcgacg ccatggaccc
     1141 tcgcgccagc cgcgaccgca ccacggcttg attcgagccc gtcggcgttt ctataatcta
     1201 cgcgctgacg ttcggacgtg ggaccaaatc aacaggaggc agtcatgaag attcgaccgt
     1261 tgcacgactg ggccgtgatc aaacaatcat ccggcgatga gcgcacggcc ggcggcttgt
     1321 acatcccgga ctcggccaag gagaaaccgc agcagggcga agtgatcgca atcggcgagg
     1381 gccgctggaa atccaaagac gaggacctca gatccaccaa gaaaaagggc gcggatgaga
     1441 agaccttcgt caaaaccacg ctcaagcccg gggacaaggt cctctacgag cgctacgcag
     1501 gctcgaaggt cacgatcaac aacgaagagt tcgtgatggt gcgggaggaa aacgtcctgg
     1561 ggattctcgc ttaaaacacg ttcaagggtt caacttgaac tcttgaaccc cttgaaccct
     1621 tgaacgttct ttgactattt cgtgacctgc gccttgatca actgatacgc cgccatcaat
     1681 ttgcgcagac gcggctcgtg gctgcggtcc ccgttgttgg cgtccgggtg cagctccttg
     1741 accaacttcc ggaacatcgc cgtgatgagc gcgggccggg tgtccggctc cacgccgagg
     1801 atggcgtacg cctcggtcag cgacgagact tcagggtgcg acgcctccgc tcttccattg
     1861 ccgccctggg attggcggaa aaaatccgac aggttgatgc gctgccggcg ccgcatcggg
     1921 cgttggcgca aggccgcgtc cacctcctcg cagcggtctt ccagaatcac gaaccgggcc
     1981 tcgaaccgct tgagcgcgtc gaagctgtcc agcgaatcga aatccgcggt cacgcgctgg
     2041 cagagcgctt ccaactcttc gagcgcctct tcgtgccgga agtaatgatc gatctggtgt
     2101 tcaaatagat gatcgagggc aaactccatc cccgcgttga tcttggcttg aagcgcatca
     2161 atgcgccccc gcaacgcgtc ctgcagcaca aaaaaccggc cggaaccgtc tttcatcgaa
     2221 cgaacaacac gcgtgtttgc gtctaaactt tcatgagtcg gtgactgacc agcacccagc
     2281 aaatcccccc ttgcccccct ttttcaaagg ggggcaagga gacgtctctg gcggcactgg
     2341 ccgcgcaggc cgttcaacag cggcctcggc tacccggttt cgggcccgta actcccccca
     2401 ttacaatcca ggcaaatgta gtccccgtcc acggggatca tcttctcccc gcaaaacgga
     2461 cactccggtg cgtcgcgttt ggggatgacc agcggcggga gttcgatttc gtcataatcc
     2521 tcgggtgcca cgatggttct cctatcgagc gtcgattcta acagaatcaa t
//
LOCUS       DBNGTT010000168         2430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1567844, whole genome shotgun sequence.
ACCESSION   DBNGTT010000168 DBNGTT010000000
VERSION     DBNGTT010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1567844"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="AC16BC_03835"
     CDS             <1..367
                     /locus_tag="AC16BC_03835"
                     /inference="COORDINATES: protein motif:HMM:NF023432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HIR3240779.1"
                     /translation="HEIETYNQLIPGAGELSATVFIEVDNPQAIKPTLDRLRGLDDGR
                     SLVLRIDGVPPIYAAFEAGHSNEEKISAVHYVRFLLDAAAQRALREGAPAALASVHPR
                     YQAETPLSEETRQQLLDDL"
     gene            369..1799
                     /locus_tag="AC16BC_03840"
     CDS             369..1799
                     /locus_tag="AC16BC_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3240780.1"
                     /translation="MDATPFLLDGRWVTGPAIAPVRNPHTGEVIAHVCQADRAQLDQA
                     IAAAERAFRETRNAPRHVRAGWLSKTAAGLAEHKTDLARLIASESGKPMQFAAGEVDR
                     AVTTFTVAAEEAKRLAGEWIPTDSTPAGEGYVAVTKRVPLGPIAAFAPFNFPLNLVAH
                     KIAPGLASGNTMVLKPPPQAPLTALRLGRILSDAGVPPGVINILPCDIPMAEALVADP
                     RFHFVTFTVSATVGWRIKTNAGKKGVLLELGGNAAAVVHVDADLPWAAKRLAVGAFAY
                     AGQICISVQRILIHEALYERFAEQFLAEVEALPVGDPLDAKTVVGPLIDQAAADRVER
                     WVADAVAGGAKALTTPARRGNVIAPIVLTNTRPAMAVSCEDVFGPVVTLAPYRAFDDA
                     LAAVNDSRYGLQAGVFTQDLRRAFESFDRLDVGGVIINDYPTFRVDHTPYGGMKDSGL
                     GREGVRYAMDAMTQIKTAVFRTGLTA"
     gene            1868..>2430
                     /locus_tag="AC16BC_03845"
     CDS             1868..>2430
                     /locus_tag="AC16BC_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3240781.1"
                     /translation="MSFTTDTRMADQTDDSSLAYQVKLPVFEGPLDLLLHLIKEHRID
                     IYDIPIALITRQYLEYLDLMKDLNLSIAGEFLVMAATLVQIKSRMLLPRDEQPESGEP
                     EEDPREELVRRLVEYQRFKDAAEALEGREAAWRDVFRREPMPMPQQEFEEYELGELSV
                     FDLLTALRDVLDRVPDPTIMEVTVEALT"
BASE COUNT          420 a          771 c          818 g          421 t
ORIGIN      
        1 ccacgaaatc gagacctaca accagttgat tccgggagcg ggcgagctct ctgccaccgt
       61 gttcatcgag gtcgataatc cgcaggcgat caaacccact ctcgatcggc tccgcggact
      121 cgacgacggt cggtcgctgg tgctgcgcat cgacggggtg ccgccgatct acgccgcctt
      181 tgaagccggg cacagcaacg aggagaagat cagcgcggtc cactacgtcc gattccttct
      241 ggacgcggcc gcgcaacgcg cgttgcgtga gggggcgccc gcggccctgg ccagcgtcca
      301 cccacggtac caggccgaga ccccgctgtc ggaggagact cgacagcagt tgttggacga
      361 tctttaacat ggacgcgacc ccgtttcttc ttgatgggcg gtgggtgacg ggcccggcca
      421 ttgcgccggt tcgcaatccg cacacgggcg aggtgatcgc tcacgtgtgt caggcggatc
      481 gcgcccagct cgaccaagcc attgccgcgg cggagcgggc gtttcgcgag acgcgaaacg
      541 cgcctcggca tgtgcgcgcc ggatggttgt ccaagaccgc ggctggcttg gctgaacaca
      601 agacggatct ggcccggctg atcgcctccg agtccggcaa gcccatgcag ttcgccgccg
      661 gtgaagtgga ccgcgcggtg accacgttta ccgtggcggc ggaagaagcc aagcgattgg
      721 ccggcgaatg gatcccgacc gattccacgc cggccggcga ggggtatgtg gccgtcacca
      781 agcgggtgcc gctcggcccg atcgcggcgt tcgcgccgtt taactttccg ctcaatcttg
      841 tggcgcacaa gatcgcgccc ggtctggcgt caggcaacac catggtgctc aagccgccgc
      901 cccaggcgcc cctgaccgcg ttgcggctcg ggcggatcct gtcggacgcg ggcgtgccgc
      961 ccggcgtcat caacatcctt ccatgcgata tcccgatggc cgaagcattg gtcgcggacc
     1021 cgcgctttca tttcgtgacg tttacggtaa gcgccacggt ggggtggcgc atcaaaacca
     1081 atgcggggaa gaaaggcgtg ttgctcgaat tgggcggcaa cgccgcggcc gtggtccacg
     1141 tggacgcgga tctcccgtgg gcggccaagc gcctggctgt tggcgcgttc gcgtacgcgg
     1201 ggcagatctg catctcggtc caacggattc tgattcacga ggcgttgtac gaacggttcg
     1261 cggagcagtt cctggccgaa gtcgaggcgc tgccggtggg ggaccctttg gacgccaaga
     1321 ccgtggtggg tccgttgatc gatcaggccg cggcggaccg cgtcgagcgg tgggtggcgg
     1381 acgccgtcgc gggcggggcc aaggctctga ccacaccggc gcgccgaggc aacgtgattg
     1441 cgccgatcgt gctgaccaac acgcggccgg ccatggccgt gagctgcgag gatgtcttcg
     1501 gcccggtggt cacgctcgcg ccgtatcgcg cgttcgatga cgccctggcc gcggtcaacg
     1561 actcgcgcta cggcctgcaa gccggcgtct tcacccagga cctgcgacgc gccttcgaat
     1621 ctttcgaccg cctggacgtg ggcggggtga tcatcaacga ctaccctacg tttcgggtgg
     1681 atcacacccc ctacgggggc atgaaggact ccgggctggg ccgcgaaggc gtcaggtacg
     1741 ccatggacgc catgacccag atcaaaaccg ccgtcttccg gaccggtctg acggcgtaaa
     1801 cggccgcggg tcggccttga cctcgtcgcc tgaaagctga taaggtccgg cccaaatcaa
     1861 ccacgtcttg agttttacga ccgacacccg catggcggat cagaccgacg actcttcatt
     1921 ggcctaccag gtcaagcttc ccgtgttcga gggaccgctc gacctcctcc tgcatttgat
     1981 caaagaacac cggatcgaca tctacgacat tcccatcgcg ttgattaccc gccagtacct
     2041 ggagtatttg gatttgatga aggatctgaa cctctcgatc gccggcgagt tcctggtgat
     2101 ggcggccacg ttggtgcaga tcaagtcgcg catgttgctg ccgcgggacg agcaacccga
     2161 gtccggcgag ccggaagagg accccaggga ggaactcgtg cggcggctgg tcgaatacca
     2221 gcgattcaag gacgcggccg aggcgttgga gggacgcgaa gccgcgtggc gggacgtgtt
     2281 ccggcgcgaa cccatgccga tgccgcagca ggagttcgaa gaatacgaac tgggcgagtt
     2341 gtcggtgttc gatctgctca ccgcgctgcg cgacgtgctc gatcgcgtgc cggacccgac
     2401 catcatggaa gtgacggtgg aggcgttgac
//
LOCUS       DBNGTT010000169         2343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3101253, whole genome shotgun sequence.
ACCESSION   DBNGTT010000169 DBNGTT010000000
VERSION     DBNGTT010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3101253"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="AC16BC_03850"
     CDS             complement(<1..840)
                     /locus_tag="AC16BC_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3240782.1"
                     /translation="MKRLLSSLLAIAVLLGSLSLAWSYQVGEVTNGGTITGTVTLRGD
                     VPQPRAFPLVLYPHGDYCKSLSDGNGHILLKEFNIDAAGRLQDAVVAVQNVSRGKPFR
                     YRDTALETTNCMFHPADVPESEQFAHHEGHLVHIHPLVTVMRDPSVLSVTNRDPIVHA
                     AQVYQAEKGNRVLSFPIPVSYKPNSGYVDLEKGKRIAQIICGMHEYMQTWAWVVDNPY
                     YAKTHKGGGFAIDKLPPGTYRVLAWHPHMTPIVKEVVVPPDGAVTLDFEFDASQVVRP
                     IYET"
     gene            1015..1980
                     /locus_tag="AC16BC_03855"
     CDS             1015..1980
                     /locus_tag="AC16BC_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240783.1"
                     /translation="MTGLALIGLIRAAHGAGDGRVTGTVAINGEAAARAVVFLQPTGG
                     PPAAMAPRERTIRQQDLRFDPDFVVVPVGTTIAFENLDKEIHNIHSRSRANRFDTGAH
                     MPETTTRVTLNNPGAVPLRCRTHERMRGLIYVAPSAYFAVTDTRGAFELTGVPAGAYR
                     AEVWHPRLSSEEQARAGTTVTVTADAAVSAVFQLHAAALPGADLTDSTDQDWRGVTDE
                     LREQLFKAVALWKSGAITAATIRVTTAQSRLYGESGLRDAIAKGLSRERAAEHEQRMD
                     HLRKRVQGLVDGSDTEASLTRAVDEIVGELNRDVGTLLHPAPDRG"
     gene            complement(2018..>2343)
                     /locus_tag="AC16BC_03860"
     CDS             complement(2018..>2343)
                     /locus_tag="AC16BC_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3240784.1"
                     /translation="TVRLSEYQYAPARIEVRKDRPVELRLVNEGNVLHEFVTDALVDT
                     TIDVEGEGVVVAARGLEELEVPAGSTVVLRFTPGKAGEFGFRCDAENPVSHHDSGMKG
                     VLTIR"
BASE COUNT          419 a          812 c          713 g          399 t
ORIGIN      
        1 ggtttcataa atcggccgga ccacctggct cgcgtcgaac tcaaagtcca gcgtgaccgc
       61 gccgtccggc gggaccacga cctctttgac gatcggcgtc atgtgtggat gccacgccaa
      121 cacgcggtag gtcccgggcg gaagtttgtc gatggcgaag ccgccgccct tgtgcgtctt
      181 ggcgtaatag gggttgtcca cgacccacgc ccacgtctgc atgtattcgt gcatgccgca
      241 gatgatctgc gcgatccgct tgcccttctc caagtccacg tacccgctgt ttggcttgta
      301 cgacacggga atcgggaagc tcagcacccg attgcccttt tccgcctgat acacctgcgc
      361 ggcgtggaca attgggtcgc ggttcgtcac ggacaacacg ctcggatccc gcatcaccgt
      421 gaccagcgga tggatgtgca cgaggtgccc ttcatggtgg gcgaactgtt cgctctccgg
      481 cacgtccgcc ggatggaaca tgcagttcgt cgtttccagc gccgtgtccc ggtaacggaa
      541 cggtttgccg cgcgagacgt tctgcaccgc caccaccgcg tcctggagcc ggccggctgc
      601 atcaatgttg aactccttga gcagaatgtg gccgttgccg tccgacagcg acttgcagta
      661 atccccgtgc ggatacagca ccaggggaaa ggcgcgcggc tggggaacgt ctccccgcaa
      721 cgtcacggtt ccggtgatgg tccctccgtt cgtcacctcg ccgacctgat acgaccacgc
      781 aagggacagg ctccccaaca gcacggcgat cgccagcaac gaagacagca gacgtttcat
      841 cggccactcc cgggtcgaac cttttacata aactaacccg gccgcgatca aatatcaacc
      901 gaccggtgga tcgttcgaat cacgcccgct ccgtggtaag atcggcccga tatgacctcc
      961 gcgccaacac ccttcacgtt cctccgcctc cgggttcccc tcgtcgtcct ggccatgacc
     1021 gggctcgcgc tcatcggact gatccgcgcg gcccacggcg cgggagatgg ccgggtcacg
     1081 ggcacggtcg cgatcaacgg agaggccgcg gcccgcgccg tggtgtttct ccaacccacg
     1141 ggcgggccac ccgcggcaat ggcgcccagg gagcggacca ttcgtcagca agaccttcga
     1201 ttcgatcccg actttgtcgt ggtcccggtc gggaccacga tcgcgttcga aaatctggac
     1261 aaggagattc acaacatcca ctcccggtcc agggccaacc gcttcgacac cggcgcccac
     1321 atgccggaga ccacgacccg cgtcacgctg aacaatcccg gggccgtgcc cctgcggtgc
     1381 cgcacgcacg agcggatgcg cgggctcata tacgtggcgc cttctgcgta ttttgccgtc
     1441 acggacacgc gcggcgcgtt tgagctgacc ggcgttccag cgggcgcgta ccgcgccgaa
     1501 gtctggcacc cgcgcctctc ttccgaagag caggctcgag ccggaacaac cgtgacggtg
     1561 accgcggacg ccgccgtctc agccgtcttt caattgcacg ccgcggcgtt gccgggcgcg
     1621 gatctgaccg actccacgga ccaggactgg cgcggcgtga cggatgagct ccgcgaacag
     1681 ctgtttaaag ccgtggcgct gtggaagagc ggcgccatca cggccgcgac catcagggtg
     1741 acgaccgcgc aatcacggct ctacggcgag tccgggctgc gcgacgccat cgccaaaggg
     1801 ctcagccgtg agcgggccgc ggaacatgaa cagcgcatgg atcatctccg caagcgcgtt
     1861 caagggctgg tggacgggtc ggacaccgag gcgagtctga cccgcgcggt ggacgagatc
     1921 gtgggcgaat tgaaccggga cgtcggaacc ctgctccacc cggcgcccga tcgcgggtga
     1981 cgctggggac aacgatcccg ccgcgacaac gcgttgctta tcggatggtc aacacgccct
     2041 tcattccgga gtcgtggtgc gagaccggat tctcggcgtc gcagcgaaaa ccgaattctc
     2101 cggccttgcc cggagtgaat cgcaagacca ccgttgaccc ggcgggcacc tccaattctt
     2161 ccagcccccg agccgccacc accacgccct caccctccac gtcgatcgtg gtgtccacca
     2221 gcgcgtccgt gacgaactcg tgcagcacgt tcccctcgtt cacgaggcgc agttcaaccg
     2281 gtcgatcctt gcgaacctcg atccgcgcgg gcgcgtactg atactcgctc aaccggaccg
     2341 tga
//
LOCUS       DBNGTT010000170         6255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1568029, whole genome shotgun sequence.
ACCESSION   DBNGTT010000170 DBNGTT010000000
VERSION     DBNGTT010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1568029"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="AC16BC_03865"
     CDS             complement(<1..1825)
                     /locus_tag="AC16BC_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3240785.1"
                     /translation="MKRIAVIGGGPHGLETLAHAVRQAGTQVAVVCDPDPGALVFRLA
                     DYGYEFAEGPRMTLAHTVEAIRAAMPLDLAVDATPVRSIRRAIAQVVPELDVIGDVAA
                     RFLWALADLPPAERPAYGLKRFKSIADEIDLSTPGELACVITETARLVTGADTVRMSV
                     WDDARQRLAALGSRPDPSPLAARAARERRTIEVSAGAAAAGWLLEDAGAAAALAAPIT
                     SEDEVLGVIEIWRAESRGAFGAEASAWLTEFVHHAVRAIKKLRTLREVRQLAQSEAAR
                     RETKTLLAADSPIHERLQRAIDSLGAILQAGAVHVYVRAPQTGDLLLQASTTLRFDQA
                     GSARLRWGAGLVGEVARLNRPAVLREDVPGDAGSGGTRGLIAVPLAAGKEAVGVLVVE
                     TPPTVEVTPRVTALLTEVGEILGASIASDAERHKMSQKVVKLSAVNEEGLQILALTDR
                     ERVLMTGAAATSMILDAEAVVIRVRQRRGERLLVGGTYGLHRDEIDAALIRLDQAITT
                     QVAQSKGFLRADRLDTFGVELPAVFPYRSVLVGPLISGDEIVGTVSAYNKVLYHSFVC
                     GAFEQLLHARRFSRVRARADREFDLDRLPVHGPEPERAERLL"
     gene            complement(1822..2649)
                     /locus_tag="AC16BC_03870"
     CDS             complement(1822..2649)
                     /locus_tag="AC16BC_03870"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3240786.1"
                     /translation="MAAKILLLDSNLRLLAALSERLTHLGYEVTTASRVHDAVLKAIV
                     VPPDLLICDVMMPELNGWEIKRLASQIPSLAATPVLFLGAQDALPVEFYDPDVGLVEA
                     AKKPIAVDALAAAVAALLARAVARARVMRHPLAGVLPLADASLIDLCQVLARRGAHGV
                     LRLTAPAWSAELTWSGGALVDAAMNGTRGNIAACAALASPAGDVEAAMEPPPAVPPRP
                     TITKPLPELLADAVGRARGAADAERSQPGPPSPPESDEEFLARLAATGLLRRTAVTR"
     gene            2839..4050
                     /locus_tag="AC16BC_03875"
     CDS             2839..4050
                     /locus_tag="AC16BC_03875"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HIR3240787.1"
                     /translation="MATDEWIDEAQRYLMATYAHVRFPVVLVKGRGTRVVDRSGREYL
                     DFAAGVAVTNLGHCHPNVTVALQKQAQRLVHTSNLYYNEPQIRLAKALVTHSFADKVF
                     FCNSGAEANEAAIKLVRKFARMSGPADRYEIITALGSFHGRTMATISASGQPKLHAGF
                     EPLLPGFVWVPYDDLGAVEKAMTDKTAAVMVEPVIGEGGVRVPHPGYLAGLRKLCTDR
                     GALLILDEVQTGVGRTGKLFAYEHAGITPDIITLAKGLANGVPIGAMLATDRVAAAFT
                     PGAHGSTQGGNPLSCAAALAVLETVLDDGYVLDNCRRMGARLVSGLKALAPRHAVIAE
                     VRGLGLLVGLALSVPARPVAMACLERGLLVIPTAETVLRLVPPLIVTHEEIDAAVGII
                     DAALGATTAHG"
     gene            4043..4972
                     /gene="argF"
                     /locus_tag="AC16BC_03880"
     CDS             4043..4972
                     /gene="argF"
                     /locus_tag="AC16BC_03880"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR3240788.1"
                     /translation="MAERDLLALRNLSSREIRRLIDRAKARTPSRVLAGKSVGLFFEK
                     ASTRTRVSFEVAIAQLGGHPVFLPAREIQLGRGETVADTARVLSRYLDALVIRTFGQD
                     RVEEWARHATIPVINGLTDAHHPCQILADLLTIDEHKGRLNGIVVAYVGDGNNIAHSL
                     LEGCAMTGMTCRVAAPAGYHPDAEIVKWAELEAHRSGGAVWIGEDPREAARGADVLYT
                     DVWTSMGQEAETATRLGVFRPYQINADLMRLAKPDAIVMHCLPAHRGEEITDDVMEGP
                     RSVIFDQAENRLHIQKAILEMLVGDGTNEGGKR"
     gene            5100..>6255
                     /locus_tag="AC16BC_03885"
     CDS             5100..>6255
                     /locus_tag="AC16BC_03885"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR3240789.1"
                     /translation="MRKLVLAYSGGLDTSVAIEWIKERYGCEVIAFCADLGQGEDLQA
                     VKAKALATGAKKVVVQDLRETFVADFVWPMLRANAVYEGSYLLGTSIARPLIAQAQMA
                     VATSERADAVGHGATGKGNDQVRFELAYQTINPSIKIVAPWREWPFKSRQDLIDYAEK
                     HEIPVTATKAKPYSVDKNLFHTSYEGGILEDPWAEPPADMFAMTVDPRRAPERAEYVE
                     IEYRAGNPVAVNGKQFSPARLLSTLNDIAGRHGIGRVDLVENRYVGMKSRGVYETPGG
                     TMLHAAHRAVESLTLDREVLHQRDALIPRYAELVYYGYWFSPERRMLQAAIDEAQRHV
                     TGTARLKLYKGAITVAGRKSPVSLYRVDLATFEEEAVYDQKDAAGFIRLNA"
BASE COUNT         1041 a         2033 c         2240 g          941 t
ORIGIN      
        1 gcaacaggcg ctcggcgcgt tccggctccg gaccgtggac ggggagccgg tccaggtcaa
       61 actcgcggtc ggcacgtgca cgtacccggg agaagcggcg agcgtgcagg agttgctcga
      121 acgcgccgca gacaaaggag tgatacaaaa ccttgttata ggccgacacg gtgccgacga
      181 tttcgtcgcc cgagatcaac gggccgacga gcacggatcg gtacgggaac acggcgggca
      241 gttccacgcc gaaagtgtcg aggcggtcgg cgcgcaggaa gcccttggac tgcgcgacct
      301 gcgtcgtgat cgcctggtcg agccggatca acgccgcgtc gatctcgtcg cggtgcaggc
      361 cgtaggtccc gcccaccaac aggcgttcgc cgcgacgttg ccgaacgcgg atgaccacgg
      421 cttcggcgtc gaggatcatc gacgtggcgg ccgcgcccgt catcagcacc cgttcgcggt
      481 cggtcaacgc gaggatttgg agcccttctt cgttgaccgc ggacaacttc acgactttct
      541 gcgacatttt gtgacgttcg gcgtcgctgg cgatcgacgc gcccagaatc tcgcccacct
      601 cggtcaacaa ggccgtgacg cgcggggtga cctcgaccgt gggcggggtt tccacgacca
      661 acacgccgac ggcctccttt ccggccgcga gcggcaccgc gatcaggccc ctcgttcccc
      721 cgcttcccgc gtctcccggg acgtcttccc gcaggaccgc cggtcggttg agcctggcca
      781 cctcgccgac cagccccgcg ccccagcgaa gacgcgcgga cccggcctgg tcgaagcgca
      841 gcgtggtgga ggcctgcagg agcaggtcgc cggtctgggg cgcgcgtacg tacacgtgca
      901 cggcgccggc ctggaggatc gcgcccagcg aatcaatggc ccgttgcagg cgctcatgga
      961 tggggctgtc cgcggcgagc agggttttgg tttcgcgccg ggccgcttcg ctctgggcga
     1021 gttgccgcac ctcgcgcagg gtgcgcagtt tcttgatcgc gcgaaccgcg tggtggacga
     1081 actcggtgag ccaggccgac gcctcagcgc cgaacgcgcc ccgactctcg gcccgccaga
     1141 tttcgatcac gcccaacacc tcgtcttcgc tggtgatcgg cgcggccagc gccgcggcgg
     1201 cgcccgcgtc ttccaacaac catccggccg cggcggcgcc ggccgaaacc tcgatcgtcc
     1261 gccgctcccg cgccgcccgg gccgccaacg gcgaagggtc gggtcgggac cccagcgccg
     1321 ccaagcgctg ccgcgcgtcg tcccacacgg acatgcggac cgtgtccgct ccggtcacca
     1381 ggcgcgcggt ttccgtgatg acgcacgcga gttcgcccgg ggtcgagagg tcgatctcgt
     1441 ccgcgatcga cttgaaccgc ttgaggccgt aggcggggcg ctcggcgggg gggaggtcgg
     1501 cgagcgccca gagaaatcgc gccgccacgt cgccgatcac gtcgagttcc ggaaccacct
     1561 gggcgatcgc gcggcggatg gatcgcaccg gggtcgcgtc cacggcgagg tccaagggca
     1621 tcgcggcccg aatcgcctcc acggtgtgag cgagcgtcat ccgcggcccc tcggcgaatt
     1681 cgtagccgta gtccgcgagc cgaaagacca gcgcgccggg atcgggatcg cagaccaccg
     1741 ccacctgcgt gcctgcctgg cggacggcgt gcgcgagggt ctccagccca tggggccctc
     1801 ccccgatgac cgcgatccgt ttcaccgggt gacggcggtt cggcggagca agccagtggc
     1861 ggccaaccga gcgaggaact cctcgtcgga ttccggcggc gacggcggac ccggttgcga
     1921 gcgctcggca tcggcggcgc cgcgcgcccg gccgacggcg tcggccagca gttcgggaag
     1981 cggtttggtg atggtcggtc gggggggcac ggccggcggc ggttccatcg cggcctcgac
     2041 gtcccccgcg ggactggcca acgcggcgca ggccgcgatg ttgccgcgcg tcccgttcat
     2101 ggcggcgtcc acgagggcgc cgccggacca ggtcagttcg gccgaccacg cgggcgcggt
     2161 gagtcgcaac acgccgtggg cgccgcgccg ggcgagcact tgacacagat cgatcagcga
     2221 ggcgtcggcc aggggcagga cgcccgccag cggatgccgc atgacgcgcg cgcgcgcgac
     2281 ggcgcgcgcc agcagggcgg ccaccgccgc cgccagcgcg tcgaccgcga tgggtttttt
     2341 ggcggcctcc accaggccca cgtcggggtc gtaaaattcc acgggcaagg cgtcctgcgc
     2401 gccgaggaag aggaccggcg tggcggccaa cgacgggatc tgggacgcca gccgcttgat
     2461 ttcccacccg ttgagttcgg gcatcatgac gtcgcagatc agtaggtccg gcgggaccac
     2521 gatcgccttg agcaccgcgt cgtggacgcg cgacgccgtg gttacctcgt agccgagatg
     2581 ggtcagccgc tcagagagcg cggctaacag acgaagattg ctatccagaa gaaggatttt
     2641 cgcggccact gagccgccct ccggcgggcg gggtcaatgt acagcggcgt cggcgggaag
     2701 tcaagaattt tcgggtgtta cgagggggaa gcccccatcc gccgacccat gaagccggtt
     2761 gcacccggca aagcgggtgt ggtacaacga gcgcgcggcc ggtcagcggc cgtcttcccg
     2821 aacaacgtga cgcgagacat ggcgacggac gagtggatcg acgaggcgca gcgttacctg
     2881 atggcgacgt acgcgcacgt gcgttttccg gtggtgctgg tcaagggccg ggggacgcgt
     2941 gtggtcgacc ggagcgggcg cgagtacctt gattttgccg cgggcgtggc cgtcaccaac
     3001 ctgggacact gccatcccaa cgtgaccgtt gcgctgcaaa agcaggcgca gcgactcgtc
     3061 cacacgtcca atctctacta caacgagccg cagatccggc tggccaaggc attggtgacg
     3121 cactcgttcg ccgacaaggt gtttttttgc aacagcggcg cggaggccaa cgaggcggcg
     3181 atcaagctcg tgcgcaagtt cgcgcggatg tccgggcccg cggaccgcta cgagattatc
     3241 accgcgctgg ggtcgttcca tggacggacc atggcgacga tcagcgcgtc gggccaacct
     3301 aaactgcacg cagggttcga gccgctgctc ccggggttcg tgtgggtccc gtacgacgat
     3361 ctcggcgcgg tggaaaaagc tatgaccgac aagaccgcgg ccgtgatggt ggagccggtg
     3421 atcggcgagg gcggcgtgcg agtgccccat cccgggtatc tggcgggcct gcgcaaattg
     3481 tgcaccgacc gcggcgcgct gttgatcttg gacgaagtgc agaccggggt agggcgaacc
     3541 ggcaagctgt ttgcctacga gcacgccggc atcacgcccg acatcatcac attggccaag
     3601 gggctggcca acggcgtgcc catcggcgcg atgctggcca cggaccgggt ggccgcggcc
     3661 ttcacccccg gggcgcacgg gtcgacccag ggcggaaacc cgctctcctg cgccgcggcg
     3721 ttggccgtgc tggagacggt gctcgacgac ggctacgtgc tggacaattg ccggcgcatg
     3781 ggggcgcgcc tggtgagcgg cctgaaggcg cttgcgccgc gccacgccgt gatcgctgaa
     3841 gtgcgaggcc tcggtctgct cgtcgggctg gcgctgtcgg tcccggcgcg cccggtggcc
     3901 atggcgtgcc tggaacgcgg cctgctcgtg atccccaccg cggaaaccgt gctgcgtctg
     3961 gtgccgccct tgatcgtgac gcacgaagaa atcgacgcgg cggtcggtat tatcgacgcg
     4021 gcgttgggag cgaccaccgc gcatggctga acgagatttg ctggcgctcc ggaacctgtc
     4081 ctcccgggag atccggcgcc tgatcgaccg cgccaaggcg cggaccccgt cgcgcgtgct
     4141 ggcgggcaag agcgtgggcc tgtttttcga aaaggcctcg acccgcaccc gcgtgtcgtt
     4201 cgaggtcgcg atcgcgcaac tcggcggtca cccggtgttc cttcccgcgc gggaaatcca
     4261 actcggccgg ggggaaaccg tggcggacac ggcgcgggtg ctctcgcggt acctggacgc
     4321 gttggtcatc cgcaccttcg gccaagaccg cgtggaggag tgggcgcgcc acgcgacgat
     4381 tcccgtgatc aacggtctga ccgacgcgca ccacccctgt cagattttgg ccgacctgct
     4441 gaccatcgac gaacacaagg ggcggctgaa cggcatcgtg gtcgcctacg tgggggacgg
     4501 gaataacatc gcgcactcgt tgctcgaagg gtgcgccatg acgggcatga cgtgccgggt
     4561 cgcggcgccg gcgggctacc atcccgatgc cgagatcgtg aagtgggcgg agttggaagc
     4621 gcaccggtcg ggcggcgcgg tgtggatcgg cgaggatccg cgcgaggcgg cgcgcggggc
     4681 ggacgtgttg tacaccgacg tgtggaccag catgggacag gaggccgaaa ccgcgacccg
     4741 actcggcgtg ttccggccct accagatcaa cgccgacctg atgcgcctgg ccaagcccga
     4801 cgcgatcgtg atgcactgct tgcccgcgca ccgcggcgaa gagatcacgg acgacgtgat
     4861 ggaaggcccg cggtcggtga tcttcgatca ggcggaaaac cgattgcaca ttcagaaggc
     4921 gatcttggag atgctggtgg gtgatggaac aaacgaaggg gggaaacggt gaaacgggga
     4981 accggtgaga aaagtactag gacgatgggg gaagtgggtg agggggtgat ggggtgagaa
     5041 gtcagaaaaa gagaacaagt agcgtccttt ctcccactct cccgctcacc cactcgccca
     5101 ttcggaagtt ggtcctcgcc tactccgggg ggctcgacac gtccgtcgcg atcgagtgga
     5161 tcaaggaacg ctacggctgc gaggtcatcg cgttttgcgc ggacctcggg cagggcgagg
     5221 atctgcaggc ggtcaaggcc aaggccctgg ccaccggggc gaagaaggtc gtggtgcagg
     5281 atttgcgcga gacgttcgtg gcggatttcg tgtggcccat gctccgcgcc aatgcggtgt
     5341 atgagggctc gtatttgctg gggacttcga tcgcccggcc cctgatcgcg caggcgcaga
     5401 tggccgtggc gaccagcgag cgcgccgacg cggtggggca cggcgcgacg gggaagggca
     5461 acgaccaggt gcgttttgaa ctcgcgtatc agaccattaa tccctcgatc aagatcgtgg
     5521 cgccgtggcg ggaatggccg ttcaagtcgc gccaggatct gatcgactac gcggagaagc
     5581 acgagatccc ggtcacggcg accaaggcca aaccgtacag cgtggacaag aacctgtttc
     5641 acaccagcta tgaaggcgga attctggaag acccgtgggc cgagcccccc gcggacatgt
     5701 tcgcgatgac cgtggatccc cggagagcgc cggagcgcgc cgaatacgtg gagatcgaat
     5761 accgcgccgg caacccggtc gcggtcaacg gcaagcagtt ctcgcccgcg cgcctcttgt
     5821 ccacgctcaa cgacatcgcc ggtcgccatg gcatcggccg ggtggacctg gtggagaacc
     5881 gctacgtggg catgaaatcg cgcggcgtgt acgagacgcc gggcggcacc atgctgcacg
     5941 cggcgcatcg cgcggtggag tcgctgacgc tggaccggga ggtgttgcat cagcgcgacg
     6001 ccctgattcc ccggtacgcc gaactggtgt actacggcta ctggttctcc ccggaacgcc
     6061 ggatgctgca ggccgcgatc gacgaggcgc agcgccacgt gacgggcacg gcccgcctca
     6121 agctctataa gggcgcgatc accgtcgcgg gccggaagtc tccggtctcg ctgtaccgcg
     6181 tcgatctggc cacgtttgag gaagaggccg tgtacgatca gaaagacgcg gcgggcttca
     6241 tcaggctcaa cgcgt
//
LOCUS       DBNGTT010000171         3867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1568249, whole genome shotgun sequence.
ACCESSION   DBNGTT010000171 DBNGTT010000000
VERSION     DBNGTT010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1568249"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..448
                     /locus_tag="AC16BC_03890"
     CDS             155..448
                     /locus_tag="AC16BC_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240790.1"
                     /translation="MNATHATTGLDDLLSTLEERVRRAATVVERLRAENARLARELES
                     AAARLGAWERRGADWERERDALGTRLEHLVRDIDDMARAHEEHEEATLSGANP"
     gene            445..756
                     /locus_tag="AC16BC_03895"
     CDS             445..756
                     /locus_tag="AC16BC_03895"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR3240791.1"
                     /translation="MTPRTVTVEIFGERYLLKGDGDPEYVRRLAGIVDRKIREAAGQL
                     PGTPLAKLAILAAVNLAHDYVKIAEDASKKDSAIEHASKRTKDLIDSIEEQFDDLDLS
                     L"
     gene            808..991
                     /gene="ssrS"
                     /locus_tag="AC16BC_03900"
     ncRNA           808..991
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16BC_03900"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1385..2932
                     /gene="rny"
                     /locus_tag="AC16BC_03905"
     CDS             1385..2932
                     /gene="rny"
                     /locus_tag="AC16BC_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR3240792.1"
                     /translation="MLLVGIVAVAGGLGLGFFISRMLTQQSAAQAKQEAGRLIEDARK
                     DIERLQKEAALEAKDKLYQARQAFEQENRDRRNELVEQERRLNQREQSLDKKMSTLDR
                     KDSDLTRKERELTARERAVTEKEAQVEQAHLQQRAQLEALSGMSAEEAKKSLIAAMEQ
                     DARFEAVKLTKRIEDEAKESAERTAREIIVSSLQRYAHDYVQEATVSVVQLPNEEMKG
                     RIIGREGRNIRALEAATGIDLVIDDTPEAILISGFDPFRREVAKVALERLMHDGRIHP
                     ARIEEVVEKVRKDTEKLMIEEAEKVLFDLGIQNMHPEMVKLLGKLKYRTSYGQNNLVH
                     AREAAIICGIMATELGLDVKLAKRAAILHDVGKAISHEEEGTHPNLGGEYARTCGEHP
                     HVINAIMAHHGDIEWTSPEGVWVAVAEGLSAARPGARREAYESYIKRLENLEQIATSF
                     KGVDKAYAIRAGREIRVIVNQDGVSEAESAQISREMAKRVENELTYPGQIKITVIRES
                     RYVEYAK"
     gene            2990..3778
                     /locus_tag="AC16BC_03910"
     CDS             2990..3778
                     /locus_tag="AC16BC_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR3240793.1"
                     /translation="MNLLLIGDIIGEPGRRILSQHLHVLKHEHRIDLAVGNAENAAGG
                     FGVTPKIAEELFAMGFDVLTTGNHVWDKKEILGYLRDHPRLLRPANYPDGVPGAGRAM
                     VTTAAGEKVGVLHLMGRVYMPGALDCPFRVGERELEKLRTETHVILVDMHGEATSEKS
                     AIGWHFDGRVSAVLGTHTHVQTADERILPQGTAFLTDVGMTGPLNSIIGVKRDEAIAK
                     FLTQMPTRFETASGPSVLSGAVVEVDGSTGRATSIRRIRITDPL"
BASE COUNT          821 a         1223 c         1268 g          555 t
ORIGIN      
        1 gcaacaggcg ctcggcgcgt tccggctccg gaccgtggac ggggagccgg tccaggtcaa
       61 actcgcggtc ggcacgtgca cgtacccggg agaagcggcg agcgtgcagg agttgctcga
      121 acgcgccaac cgacttacgc ccctggactg accgatgaac gcgacgcacg ccacgaccgg
      181 cctcgatgac ctgctcagca cgttggaaga gcgggtgcgc cgggcggcca ccgtcgtcga
      241 gcggctacgg gccgagaacg cgcggctggc gcgtgaactc gagtcagccg cggcgcggct
      301 cggcgcgtgg gaacggcgcg gcgcggattg ggaacgggaa cgggacgcgc tcgggacgcg
      361 cctggaacac ctggtgcgcg acatcgacga catggcgcgc gcccacgaag aacacgagga
      421 ggccacctta tccggagcca acccatgacc ccacgaaccg tcacggtcga aatttttggc
      481 gaacggtatc tcctcaaggg tgacggcgac cccgagtatg tccgccggct cgccgggatc
      541 gtcgaccgca agatccggga agccgccggc cagttgcccg gcacgcccct ggccaaactc
      601 gcgattctgg cggccgtcaa cctcgcccac gattacgtga aaatcgccga agacgcgtcg
      661 aaaaaagaca gcgccatcga acacgcgtcc aaacggacga aggatctcat cgacagcatc
      721 gaggagcaat tcgatgacct cgatctctcg ctgtagcgcc tcgaattgct tgaattcccc
      781 ccgacctttt gctaagctcg gattcgtgtg cccctgctgt gtgcgcgagc cggcgggatg
      841 tttgtgccaa cagtttcgaa tcgggagctt tcccggcaga gatggtgtgc acgccccgcg
      901 gagcggggaa gcctaaagtc tctccgaagg cacccactga aggccctttc ggcttcaaac
      961 gatttcgccg ccacggcatg gcgggggcca ctcttgaacg gacaaacggg gttgtcggag
     1021 gacgacgccc gggacggata gagagggtga tggacgctgc gttcttcgcc ggatcagcga
     1081 gtggaaaatc agccgagacc ggcacggttt cggctagtgg tataaacgtc aggtccgtgg
     1141 tggctccaca cgagtgcgtg gactgcaatt cgtcgccacc cacgatctga tcgcgtctcc
     1201 acaccacgtc cccgctcgcc accctcccgc gccccacgtc tcgccgacga cctcgcacag
     1261 gggtctgtgc agtgccatga aggccaacgt caacgacgtc ttgcagcctc ggcgcgggac
     1321 agcgcgccta cgttgcatca cgcaagcgag gtagggagga ctaaccatta ctacgacact
     1381 cggaatgttg ctggtcggga tcgtcgcggt ggccggaggg ctggggctcg ggtttttcat
     1441 ctcgcgcatg ttgacccagc aatcagcggc ccaggccaag caggaggccg gacggctcat
     1501 cgaggacgca cgcaaggata ttgaacggct gcagaaggaa gcggcgctcg aagcaaaaga
     1561 taagctctac caagcccgac aggcgtttga acaggaaaac cgcgaccgac ggaacgaact
     1621 ggtcgagcag gaacgccgtc tgaaccagcg cgagcagtcg ctcgacaaaa aaatgagtac
     1681 gctcgaccgc aaggattccg acctcacccg gaaagagcgc gaactgaccg cccgcgagcg
     1741 cgcggtgacc gagaaggaag cccaggtcga gcaggcgcac ctccagcagc gggcgcaact
     1801 cgaagcactc agcgggatga gcgccgaaga agccaaaaag agcctgatcg ccgccatgga
     1861 acaggacgcg cggttcgagg cggtcaagct caccaagcgc atcgaggacg aggccaagga
     1921 atccgcggaa cgcacggccc gcgagatcat cgtgtcgtcc ctgcaacgct acgcccacga
     1981 ctacgtccag gaggccacgg tctccgtcgt ccagctgccc aacgaagaaa tgaaggggcg
     2041 gatcatcggc cgcgaagggc gcaacatccg cgcgctggaa gccgcgaccg gcatcgacct
     2101 ggtcatcgac gacacgccgg aggccatcct catctcgggc ttcgacccgt tccgacgcga
     2161 agtggccaag gtcgcgctgg aacgcctgat gcacgacgga cgcattcacc cggcgcgcat
     2221 cgaggaggtg gtggagaagg tccgcaagga caccgaaaaa ctcatgatcg aagaagccga
     2281 aaaggtcctg ttcgacctgg gcatccagaa catgcacccg gagatggtca agttgctggg
     2341 caaactcaag taccgcacca gctacgggca gaacaacttg gtccacgcgc gggaagctgc
     2401 gatcatttgc ggcatcatgg cgaccgaact ggggttggac gtcaaactcg ccaaacgcgc
     2461 ggcgatcctg cacgacgtgg gcaaggccat cagccacgaa gaggaaggca cgcaccccaa
     2521 cctgggcggc gaatacgcgc gcacgtgcgg cgagcatccg cacgtcatca acgcgatcat
     2581 ggcgcaccac ggcgacattg agtggacctc gcccgaaggc gtgtgggtcg cggtggcgga
     2641 aggcctgtcg gcggcgcgcc ccggggcgcg ccgcgaagcg tacgagtcct acatcaagcg
     2701 cttggaaaac ttggagcaga tcgccacctc gttcaaaggc gtggacaagg cgtacgcgat
     2761 ccgtgcgggc cgcgagatcc gcgtcattgt gaatcaggac ggcgtgtccg aagccgagtc
     2821 cgcgcagatt tcgcgcgaga tggccaaacg cgtggaaaac gagctgacgt acccgggaca
     2881 gatcaagatc accgtgatcc gcgaaagccg atacgtggag tatgcgaaat agggcatccg
     2941 gccgtgtagg ggcgcggcat gccgcgcccc tacacccaaa ccgcaaccaa tgaatcttct
     3001 tctcatcggc gacatcatcg gcgaacccgg ccggcggatc ttgagccagc acctccacgt
     3061 gctgaagcat gaacaccgca tcgacctggc ggtcggcaac gcagaaaacg cggcgggcgg
     3121 gttcggcgtc accccgaaaa tcgcggagga gttgttcgcg atggggttcg acgtgttgac
     3181 caccggcaac cacgtctggg acaagaagga gatcctcggc tacctgcgcg accatcctcg
     3241 gctgctgcgg cccgccaact atccggacgg tgtgccgggc gccggacgcg cgatggtgac
     3301 cacggcggcc ggcgagaagg tcggcgtcct gcacctgatg ggccgcgtgt acatgccggg
     3361 cgcgctcgac tgcccgtttc gcgtgggcga acgggaactg gaaaagcttc gcaccgaaac
     3421 gcacgtgatt ctggtggaca tgcacggcga ggccacgtcc gaaaagtccg cgatcggctg
     3481 gcacttcgac gggagagtca gcgcggtgct cgggacccac acgcacgtac agaccgcgga
     3541 tgagcgcatc ctgccgcagg gcacggcgtt tctgaccgac gtgggcatga ccggaccgct
     3601 caattcgatc atcggcgtga agagggacga ggccatcgcc aagtttttga cgcagatgcc
     3661 gacgcgattt gaaaccgcca gcggcccgtc ggtgctgtcg ggcgccgtcg tggaagtcga
     3721 cggttcgacc ggccgcgcca cgagcatccg tcgcatccgc atcaccgacc cgttataggg
     3781 tgccgaggaa gcggtgaccg tggttgcttc gccacgcaga gggcgcgttc gacaggctca
     3841 ccacgaacgg cgtattcgcc gggctcg
//
LOCUS       DBNGTT010000172         2377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2974146, whole genome shotgun sequence.
ACCESSION   DBNGTT010000172 DBNGTT010000000
VERSION     DBNGTT010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2974146"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC16BC_03915"
     CDS             <1..187
                     /locus_tag="AC16BC_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240794.1"
                     /translation="SNLFVRFIQRGGESLAVLHEPVAFFIPEHIPDPSRRTPDEQLCV
                     EVTVPQKGPPRPIRLGV"
     gene            352..1002
                     /locus_tag="AC16BC_03920"
     CDS             352..1002
                     /locus_tag="AC16BC_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3240795.1"
                     /translation="MAMRWLALTLVLMAAVLSDLDGIAAEAGSMEMPMSGGGMRGHMM
                     GGRLPPGIEPDALPEPQSEGAALLSRYCTQCHNLPSPGLHSADEWFSVTTRMFHRMVM
                     TSNPRHRGGMMRRRMPHVLAPTSAEQAQIVAYLTQHALRPAPPRTPETPETPEMALFR
                     QTCSRCHALPDSASHTADEWPLVVERMLKNMAKMGKAGVSDQERDAIVAYLQGRAR"
     gene            complement(1060..2088)
                     /locus_tag="AC16BC_03925"
     CDS             complement(1060..2088)
                     /locus_tag="AC16BC_03925"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3240796.1"
                     /translation="MIVKKSKYTVAVIGATGAVGKESLEILEERKFPLAGLRLFASKR
                     SAGGVLTCQGRNWTVEELTPTSDLTGVDFAFISATDAISREWGPRLGAAGVVAIDDSA
                     VFRMDPGVPLVVPEVNAQALASASRGIVAIPNCTTTPLVMALKPLHEAATIKRVVVTT
                     FQSVSGTGAAAMDELVEQTKALLTFQEVKAEVYPYQIAFNLLPHVGSFGEGGDCSEEV
                     KIVRETQKILGEPAMRITATTVRVPVMRCHSEAVNVELARPLTANDARAALSEMPGVV
                     VFDDPLRKLYPMPLDASGKDEVFIGRIREDVSVTNGLNLWLVSDNLRKGAALNAIQIA
                     ETLIARAS"
     gene            complement(2171..>2377)
                     /locus_tag="AC16BC_03930"
     CDS             complement(2171..>2377)
                     /locus_tag="AC16BC_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3240797.1"
                     /translation="GAVNDKGEPIEFNGTYTWVTSSTLRFKPDGTLKPDSVYQVTVWS
                     AKAKDGSELQTAPYRLPFSTTGAK"
BASE COUNT          422 a          783 c          737 g          435 t
ORIGIN      
        1 ctctaatctg ttcgtgcggt ttatccagcg cggaggcgaa tccctggcgg tgctgcatga
       61 acccgtagcg ttttttatcc ccgagcacat cccggatccc tcgcgtcgca cgccggacga
      121 gcaattgtgt gtggaagtga ccgttcccca gaaaggcccg cctcggccga ttcggttggg
      181 agtatagaag ggcgtcgtcc cgatctaacc gatgaacctg taacacccca cctctgggct
      241 tttctgaagg gcggaccggt cacccaacgc gcggcacaac cccggcggtc tccgctggat
      301 tctccggccg tgatctcgta gcatcggggt caatcggacc gaggagacgg tatggcgatg
      361 cgatggctcg cgctcacgtt ggtactgatg gccgccgttc tcagcgatct cgatgggatt
      421 gcggccgagg cgggttcaat ggagatgccc atgagcggcg ggggcatgcg cgggcacatg
      481 atgggtggtc ggctcccgcc aggcatcgag cccgacgcat tgcccgagcc ccagagcgaa
      541 ggcgcggccc tgttgagccg ttactgcacc cagtgccaca accttcccag cccgggtctg
      601 cactcggctg atgagtggtt ctccgtgaca acacgaatgt ttcaccggat ggtgatgacc
      661 tcgaatccac gccaccgtgg cggcatgatg cggcggagaa tgccgcacgt ccttgctccc
      721 acctccgcgg agcaggcaca gattgtcgct tacctcacgc agcacgccct gcgccccgca
      781 ccgccgcgca cgcccgagac tcccgagacc cctgaaatgg ccctcttccg gcaaacgtgc
      841 tcgcgctgtc acgcccttcc cgacagcgcc tcgcatacgg ctgacgagtg gccgttggtg
      901 gtggagcgga tgctcaagaa catggcgaag atgggaaagg cgggggtcag cgatcaggaa
      961 cgggacgcaa tcgttgccta ccttcagggg cgggctcgtt agcgcggggc ggagcgcctt
     1021 ccggcgcaag ttcggcgttt acgagacgag ttgccgggct caggacgccc gcgcgatcag
     1081 cgtttcggcg atctggattg cgttgagggc cgcgcccttg cggaggttgt cggagacgag
     1141 ccagagattg aggccgttgg tcaccgacac atcctcgcga atgcgaccga tgaagacctc
     1201 gtcttttccg gaggcatcca gcggcattgg atagagcttt cgcagcgggt catcgaacac
     1261 caccacgcca ggcatctccg aaagcgccgc gcgcgcgtcg ttggccgtga gcggcctcgc
     1321 cagctccacg ttcacggctt cggaatggca gcgcatcacc gggacccgca ccgtggtcgc
     1381 cgtgatccgc atcgcgggct cgccgaggat cttctgtgtc tcgcgcacga ttttgacttc
     1441 ctcggaacaa tctcccccct cgccgaagga ccccacgtgc gggaggaggt tgaacgcgat
     1501 ctgatacgga tagacctcgg ctttgacctc ctgaaacgtg agcaaggcct tggtctgctc
     1561 gaccaattcg tccatggccg cggccccggt cccagacacg gattgaaacg tcgtcaccac
     1621 cacacgcttg atagtcgcgg cttcgtgaag gggcttgagc gccatcacca gcggcgtggt
     1681 cgtacagttc ggaatggcga cgatcccgcg agaggccgag gccaatgcct gagcattgac
     1741 ttccggcacc accagcggca ccccggggtc catacggaac acggcgctgt catcgatggc
     1801 caccacgccg gccgctccca ggcgaggccc ccattcccgg ctgattgcgt ccgtcgccga
     1861 gatgaaggcg aaatccacgc cggtcaaatc gctcgtcggc gtcaactctt ccacggtcca
     1921 attgcggccc tgacaggtca ggaccccgcc cgccgaacgt ttggaggcga acaaccgcaa
     1981 acccgccagc gggaacttgc gttcctccag gatttccagg ctctctttgc caacggcccc
     2041 ggttgcgccg atcacggcta ccgtgtattt ggatttcttg acgatcatgg gctccatccc
     2101 ctgagaacaa cgcgaccgga gcgcgcggcc gcgcgctccg gtcgatcacg catccgcgac
     2161 gaaatcgtcg ctatttggct cccgtcgttg agaacggcag gcggtagggc gcggtctgga
     2221 gttcggaccc gtccttagcc tttgccgacc acaccgtgac ctgatagacc gaatcgggct
     2281 tgagggtgcc gtccggtttg aaacgcaacg tggaggacgt cacccacgtg tacgtcccgt
     2341 tgaactcgat cggctcaccc ttgtcgttca cggcccc
//
LOCUS       DBNGTT010000173        10238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_802182, whole genome shotgun sequence.
ACCESSION   DBNGTT010000173 DBNGTT010000000
VERSION     DBNGTT010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_802182"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1052)
                     /gene="argB"
                     /locus_tag="AC16BC_03935"
     CDS             complement(192..1052)
                     /gene="argB"
                     /locus_tag="AC16BC_03935"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR3240798.1"
                     /translation="MKRLIEKANTLVEALPYLRAFAGKTVVIKYGGHAQVDEALKAGF
                     AEDVVLMKYIGVNPVVVHGGGPQITDMMARLGKKPTFVDGVRVTDRETMEIVEMVLGG
                     VINKEIVELISRHGGRAVGLSGKDGRLISAKPLKSSGPKRVDLGQVGEVESVDPQLLI
                     DLTSNRYIPVIAPIGVDRDGRTYNINADLVAGAVARALKAEKLLVLTDVAGILDAKRA
                     LIPTVSRKEIQRLIKSGTITSGMLPKVQACLTAVEGGVAKAHIIDGRVPHALLLEIFT
                     KEGIGTEIIA"
     gene            complement(1208..2572)
                     /gene="hslU"
                     /locus_tag="AC16BC_03940"
     CDS             complement(1208..2572)
                     /gene="hslU"
                     /locus_tag="AC16BC_03940"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR3240799.1"
                     /translation="MTPRQIVEALDRYVVGQHAAKRMVALALRNRWRRRRLAPDLRDE
                     VLPKNIIMMGPTGVGKTEISRRLAKLAHAPFLKVEASKFTEVGYVGRDVESMVRDLVE
                     LTITMVKTESTGRVQDKAAVQAEERLLDLLLPPPALRHPTGVFDASRETAAPPGYSDT
                     YDQTRQKLRQQLRDGKLEERTVELEVKEKAAALPVGIISNVGMDEIEGNLRDMLGNLF
                     PGKKKPRRMTVAEARQVLVQEEAAKLIDMDEVTREAIRRAEQDGIIFLDEIDKIAGRE
                     RGQGPDVSREGVQRDLRPIVEGSTVNTKHGPVKTDHILFIAAGAFHISKPSDLIPELQ
                     GRFPLRVELEPLTKADFVRILTEPRNALIRQYIALLATEDVDLTFTPDAIEEIAAIAV
                     MANERMENIGARRLFTVMERLLDAVSFDAPELADKKIAIDAAYVKQALADVVKDQDLS
                     RYIL"
     gene            complement(2626..3168)
                     /gene="hslV"
                     /locus_tag="AC16BC_03945"
     CDS             complement(2626..3168)
                     /gene="hslV"
                     /locus_tag="AC16BC_03945"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR3240800.1"
                     /translation="MTPRIRSTTVLCVRLDKDVAMACDGQVTMGTTVMKSNARKIRRM
                     SNDRVLAGFAGSTADALALFDKFEAKLSEFRGNLSRAAVELAKDWRTDRVLRRLEALL
                     AVADLSGSLLISGTGDVIEPEDGILAIGSGGPYALAAARALLGQPGMTAETIATRAMK
                     IAADIDIYTNDSIVVETLRG"
     gene            complement(3165..4100)
                     /locus_tag="AC16BC_03950"
     CDS             complement(3165..4100)
                     /locus_tag="AC16BC_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HIR3240801.1"
                     /translation="MEDPISRVLNILAMERGASGETLRAYASDLRQFAAFLRDPDRAM
                     DASQPLGTGPVADAGRILAASRLDIRAYLACMKHRGAKKSSMMRKLAAVRTLYRYLWR
                     DGTIASNPASSVATPKQDKPLPKALSVDAAAAVVEAPSGSGANVLRDRAVLETFYSTG
                     ARLSELTGLNVGDIEWDAGLVRLFGKGRKERVVPIGSRALAAIRAYREAIGMERATGA
                     VFVGESGRRLSSRTIARIVKSSARAAGEPDASPHTLRHSFATHLLEGGADLRAIQDML
                     GHASLATTQRYTHLTVDHLMSVYDAAHPRARRKAS"
     gene            complement(4073..5398)
                     /gene="trmFO"
                     /locus_tag="AC16BC_03955"
     CDS             complement(4073..5398)
                     /gene="trmFO"
                     /locus_tag="AC16BC_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR3240802.1"
                     /translation="MAQTSPELTVLGGGLAGCEAAWQAAERGVTVVLYEMRPARPTAA
                     HKTDQLAELVCSNSLGSAEITNAAGILKEEMRRLGSLVVRVADTCRVPAGSALAVDRV
                     EFAARITKEISSHPNITLIREELPSIPEQGPVIVATGPLTSDALAESLKKLTKAERLY
                     FFDAISPIVDGETIDRSIAFAASRYNKGGDDYLNCPMTEAEYNDFYAALLAAEKVPTR
                     EFEKTPYFEGCMPIEVQAERGRQTLLFGPMKPVGLVDPRSGQRPFAVVQLRPEDVHGQ
                     AFNIVGFQTKLKWPEQKRVFRMIPGLEQAEFLRFGSLHRNTFINAPLLLRETLQLRYD
                     SRIFFAGQIVGVEGYTESAGMGLLAGINAANLIHHKALVIPPPTTTLGTLITYLTTSD
                     PRHFQPMNVNLGLFPPLPTRVAQRDVRRQKIVDRALSDLDRWKTQSLAS"
     gene            5606..6502
                     /locus_tag="AC16BC_03960"
     CDS             5606..6502
                     /locus_tag="AC16BC_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240803.1"
                     /translation="MKLRRLVVLPFLGAVGVAMLNVSRDQALSNALSWGPVWILLALG
                     VWWALAPYIRPHLAPFFVARAGMMVIVALLAIITVSAIETYREVRPLPSNPGIDITEA
                     PPGVFPDVPEMFLIDFGSNVSKVDRRLLEWQVPFEDMSFLRSFHGLLRGRIPLQIFFD
                     EHGALKINATIYGSNREKVAEIENSFLKIRNPKWDYNSDSTAFEIVDADGIPYFQVYR
                     YEANAIAIRGVFYTSAGERIISTDRTVIFDDKNAVMRPQPLFLYPRRFHWGERVRRPF
                     IVLDVDRQKSMSPVDSRPNRQM"
     gene            complement(6771..7049)
                     /locus_tag="AC16BC_03965"
     CDS             complement(6771..7049)
                     /locus_tag="AC16BC_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240804.1"
                     /translation="MSEQEQDLDELLGRTRCARCGQVLDGQIECPFCSIFPDPPRAQR
                     LPTWVFFTACFLTSPLSLPVILTTRRLTGFQKVLATSGLFGWLGLWWV"
     gene            complement(7053..8087)
                     /locus_tag="AC16BC_03970"
     CDS             complement(7053..8087)
                     /locus_tag="AC16BC_03970"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3240805.1"
                     /translation="MNNLIELVSFDGITPGQERTPDAASDEELLDAYSRAVTGAADRV
                     SPSVVNIDVHRHVAGSARRRAFEAQGSGSGFLFTPDGFIVTNSHVVRGADQLTVTLVD
                     GRRAQADLVGDDPDTDLAVIRVGAPGLPAVTFGDSHAVRVGQLAIAIGNPYGFQCTVT
                     AGVVSALGRSLRSTSGRLIDNVLQTDAALNPGTSGGPLVDSRGRVIGVNTAVIRGAQG
                     LCFAIGINTAAFVAGRLIKDGRITRGYLGLAGQDTPLHRRLVRFHGLAVERGILVASV
                     EPGSPAERAGILPGDVLIAFGDRPVDGIDALHRLLADTAVGVPSPVTVIRGAERLAVT
                     IVPEAARPRQ"
     gene            complement(8242..8976)
                     /locus_tag="AC16BC_03975"
     CDS             complement(8242..8976)
                     /locus_tag="AC16BC_03975"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008127 - quercetin 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3240806.1"
                     /translation="MPATLTHIQRSADRFVTSIDWLHGKHSFSFGPHYDPKNTHHGLL
                     LVNNEDVVRPGTGFRTHPHQDMEIVTWVLDGELEHKDTLGNKGIIYPGLAQRMSAGTG
                     IWHSEMNPSGDAPVHFVQMWVIPDTERINPAYEQLDINPQIAKGGLVPLASGRGHDAA
                     ISIRQKDAVLWVGRLKPGETVTIPDARFIHVFVPKGAAELEGVGRLEAGDSVRLVAAG
                     ARRFTADAAGGAEVLVWEMNSHLRGW"
     gene            complement(9051..9404)
                     /locus_tag="AC16BC_03980"
     CDS             complement(9051..9404)
                     /locus_tag="AC16BC_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240807.1"
                     /translation="MHEAERAGAQTFFLAHVFFLVVFMALVAALVVVLITRRKPLPLL
                     LVILPLGLIFATAFLARPFPLAHQIVNIGYDALLVFNTVYFWRTGPRFVGPLFLVGVL
                     GTALDFAMHFVINIA"
     gene            complement(9610..9999)
                     /gene="crcB"
                     /locus_tag="AC16BC_03985"
     CDS             complement(9610..9999)
                     /gene="crcB"
                     /locus_tag="AC16BC_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR3240808.1"
                     /translation="MRQLIPYLLVGIGGFLGANARFIVGTWVTSTLGSRVPLGTFVVN
                     VSGCLLIGILGTVIVDKLVARPEHVQWLLAIGFLGAYTTFSSFAWENHLLFLEGRWLT
                     AALYMALSVFAGLIAVQLGVLLARAWF"
BASE COUNT         1870 a         3227 c         3199 g         1942 t
ORIGIN      
        1 cggattttga gtccgccgtg tctgccattc caccacttcg gcgtctggtc cgtcgatagg
       61 gggccatctg cggcgttgtc gcttcggaag cttgtcctca cgtacgactc agtacgttcc
      121 gggaagcttc ctcgctccgc cttgcatctg gccccctctc aacggccaaa ctcggcggac
      181 aaggacaaca ctcaggcgat gatctcggtg ccgatgccct ctttcgtaaa gatttccaac
      241 aataaggcat gtggcacgcg tccgtcaatg atgtgggcct tggccacgcc gccttccacc
      301 gcggtgaggc aggcctggac cttgggcagc atgccggagg tgatggtgcc ggacttgatc
      361 aggcgctgga tctccttgcg cgacacggtg gggatgagcg cgcgtttggc gtccaggatg
      421 ccggccacgt cggtcaacac cagcaatttt tccgccttca gcgcgcgcgc caccgcgccc
      481 gccaccaggt ccgcgttgat gttgtaggtg cggccgtcgc ggtccacgcc gatgggcgcg
      541 atcacgggga tatagcgatt ggacgtgagg tcgatcaaga gttgcgggtc caccgattcg
      601 acctcgccca cctgacccaa gtccacgcgt ttggggcccg aggatttgag gggcttggcc
      661 gagatcaacc ggccgtcctt gccgctcaac cccacggcgc ggccgccgtg acgggagatc
      721 aactccacga tttccttgtt gatgaccccg cccaggacca tctccacgat ctccatggtc
      781 tcgcggtcgg tgacgcgcac gccgtccacg aacgtgggct tcttgcccag gcgcgccatc
      841 atgtccgtga tctggggccc gccgccgtgc accaccaccg ggttcacccc gatgtacttc
      901 atgagcacca cgtcttcggc gaatccggct ttgagcgctt cgtccacctg cgcgtggccg
      961 ccgtatttga tgacgactgt ttttcccgcg aaggcgcgta aatacggaag cgcttcgact
     1021 aaggtattgg ctttttcgat cagtcgcttc atatggctaa gagcccgtcc atgaaaggcc
     1081 atctgcggcg ttgccgctgc gcatccggtg ctcacgtacc cacctcgtac gctccgctcc
     1141 ggtgctcgcg gcgcctggca tctggcgctt tcctgaacgg gcttcgctcc cgcgatattc
     1201 aaacatatta cagaatgtag cggctcagat cttggtcttt gacgacatcg gccaaggcct
     1261 gtttcacgta ggccgcgtcg atggcgattt tcttgtccgc cagttccggc gcgtcgaagg
     1321 acacggcgtc gagcagacgt tccatcacgg taaacaagcg ccgcgcgccg atgttttcca
     1381 tccgctcgtt ggccatcacc gcgatcgccg cgatttcttc gatggcgtcg ggcgtgaacg
     1441 tcaggtccac gtcctcggtg gcgagcaggg cgatgtactg acggatcagg gcattgcgcg
     1501 gctcggtcaa gatgcggaca aagtcggcct tggtgagcgg ctcgagttcc acccgcaacg
     1561 ggaagcgtcc ctgcaactcg gggatcagat ccgacggctt gctgatgtgg aacgcgcccg
     1621 ccgcgatgaa caggatgtgg tcggtcttga ccggtccgtg tttggtgttg accgtggagc
     1681 cttccacgat gggccgcagg tcgcgctgca caccctcgcg cgagacgtcg ggcccctggc
     1741 cgcgttcgcg gcccgcgatc ttgtcgatct catcgaggaa aatgatgccg tcctgctccg
     1801 cgcgccggat cgcttcgcgc gtcacctcgt ccatgtcgat cagtttggcg gcttcctcct
     1861 ggaccagcac ctggcgcgct tcggccacgg tcatgcgacg cggcttcttt tttcccggaa
     1921 acagattgcc cagcatgtcg cgcaggttcc cttcgatctc gtccatcccg acattggaaa
     1981 tgatgcccac cggcaaggcc gcggccttct ctttgacctc gagttccacg gtccgctctt
     2041 ccagtttgcc gtcgcgcaac tgttggcgca gcttctgccg cgtctggtcg taggtgtccg
     2101 agtacccggg cggggccgcg gtttcccgcg acgcgtcgaa cacgccggtg gggtgtcgca
     2161 gcgcgggcgg cggcaagagc aggtcaagca ggcgctcctc ggcctgcacc gcggccttgt
     2221 cctggacgcg ccccgtggat tcggtcttga ccatggtgat ggtcagctcc actaggtcgc
     2281 gcaccatcga ctccacgtcg cgccccacgt agccgacctc cgtgaacttg gaggcttcga
     2341 ccttgaggaa cggcgcgtgc gcgagtttgg ccagccgccg cgaaatttcg gtcttgccca
     2401 cgccggtcgg gcccatcatg atgatgttct tgggcaacac ctcgtcgcgc aggtccgggg
     2461 ccaggcggcg ccgtcgccat cggttgcgca aggccaacgc gaccatgcgt ttggccgcgt
     2521 gctggcccac cacgtaacga tccagcgcct ccacgatctg gcggggcgtc atgtcgtcga
     2581 tcgcgggcaa cgaatccgta ttgctggatg ttgacgaatc cacggctaac ccctcagcgt
     2641 ctcgaccaca attgaatcgt tcgtgtagat atcgatatcc gcggcgatct tcatggcgcg
     2701 cgtcgcaatc gtctcggcgg tcatgcccgg ctggcccagc aacgcgcgcg ccgcggcgag
     2761 cgcgtagggt ccgccggagc cgatggccag aatcccgtcc tcgggctcga tcacgtcgcc
     2821 ggtgcccgag atcagcagcg acccggacag gtccgcgacc gcgagcaacg cttcgagccg
     2881 gcgcaggacg cggtccgtgc gccagtcctt ggccagttcc accgcggccc ggctcaaatt
     2941 gccgcggaac tccgacaact tggcttcgaa cttgtcgaag agggccaatg cgtccgcggt
     3001 ggacccggca aaacccgcca gcacgcgatc gttggacatc cgccggatct tgcgcgcgtt
     3061 ggacttcatc acggtcgtgc ccatggtgac ctggccgtcg caggccatgg ccacgtcttt
     3121 atcgaggcgc acgcagagca ccgtggtgga tcgaatccgc ggggtcatga cgctttgcgc
     3181 cgcgcgcggg ggtgcgccgc gtcgtacacc gacatcaaat gatccacggt caaatgcgtg
     3241 tagcgctgcg tggtggccaa tgacgcgtgg cccagcatgt cctggatcgc acgcagatcc
     3301 gcgccgccct ccaggaggtg tgtggcgaaa ctgtggcgca aggtatgcgg gctggcgtcg
     3361 ggttctccgg cggctcgcgc cgaggatttg acgatgcgcg caatggtccg gctcgaaagc
     3421 cgccgcccgg actcgccgac gaacaccgcg cccgtggccc gttccatgcc gatcgcctcg
     3481 cggtaggcgc ggatcgcggc caaggccctg gacccgatcg ggaccacgcg ctccttgcgt
     3541 cctttcccga ataggcgcac gagccccgcg tcccactcga tgtcgccgac gttgaggccg
     3601 gtcaactcgg acaggcgcgc gcccgtggaa tagaaggttt cgagcaccgc gcgatcgcgc
     3661 aatacgttcg cgcccgagcc gctcggggcc tcgaccacgg ctgctgccgc gtccaccgac
     3721 aacgccttgg gcagcggttt gtcctgtttg ggcgtggcca cgctcgacgc cggattcgag
     3781 gcgatcgtgc cgtcgcgcca gagataccga tagagcgtcc gcacggccgc gagttttcgc
     3841 atcatggacg attttttcgc gccccgatgt ttcatgcacg ccaggtacgc gcgaatgtcc
     3901 aggcggcttg ccgccagaat ccggccggcg tccgcgacag ggccggttcc caggggctgc
     3961 gacgcgtcca tggcgcggtc cgggtcgcgc agaaacgccg cgaactgccg aaggtccgag
     4021 gcgtaggccc gcagggtctc gcccgaggcg ccgcgctcca tcgccaggat gttcaggacg
     4081 cgagagattg ggtcttccac cggtccaggt cgctcaatgc gcggtccacg atcttttgcc
     4141 gccgcacgtc tcgctgcgcc acgcgcgtgg gcagcggagg aaacaaaccc aggtttacgt
     4201 tcatgggctg gaaatgccgg ggatcgctgg tcgtcaggta cgtgatcagc gtccccaggg
     4261 ttgtggtcgg cggcgggatc accagcgctt tgtggtgaat gagattcgcc gcattgatgc
     4321 cggcgagcag gcccatgccc gcggattcgg tgtagccttc cacgcccacg atctggcccg
     4381 cgaagaaaat ccgggagtcg tagcgcagtt gcagggtttc gcgcagcagg agcggcgcgt
     4441 tgatgaacgt gttgcggtgc aggctgccga agcgcaggaa ctcggcctgc tccagccccg
     4501 ggatcatgcg gaacacgcgc ttttgttccg gccacttgag tttggtctga aagcccacga
     4561 tgttgaaggc ctggccgtgc acgtcttcgg gtcgcaactg cacgaccgcg aacggccgtt
     4621 ggccgctgcg cggatcgacc aggcccaccg gcttcatcgg gccgaagagc agggtctgcc
     4681 ggccgcgctc ggcctgcact tcgatcggca tgcagccctc gaaatacggc gtcttctcga
     4741 actcccgggt cggcaccttt tccgcggcca gcaatgcggc gtagaagtcg ttgtactcgg
     4801 cctcggtcat ggggcagttc aagtaatcgt cgccgccctt gttgtagcgg gacgcggcga
     4861 acgcgatcga gcggtcgatg gtttcgccgt ccacgatggg cgagatcgcg tcgaaaaagt
     4921 acaggcgctc ggccttggtc aatttcttga gcgactcggc caacgcgtcg gaggtcaacg
     4981 gcccggtcgc cacgatcacc ggaccctgtt ccggaatgct cggcagctcc tcgcggatga
     5041 gtgtgatgtt cgggtgcgag gagatctcct tggtgatgcg cgcggcgaac tccacgcggt
     5101 ccacggcgag tgcggagccc gcgggcacgc ggcacgtgtc ggccacgcgc accaccagcg
     5161 agcccagccg ccgcatctct tccttgagga tgccggcggc gttggtgatc tccgcggaac
     5221 ccaacgagtt cgagcagacc agttcggcga gctgatcggt cttgtgcgcg gcggtgggcc
     5281 gggcggggcg catctcgtac aacacgaccg tgacgccgcg ttccgcggcc tgccacgccg
     5341 cttcgcagcc cgccaatccg ccgccgagga cggtcaattc cggagaagtc tgtgccatga
     5401 agtatcccgc gcgggacaag cgattctacg ggccgggcgg aaagcgtgtc aagcaaacgc
     5461 cgccctgtgt actgtcctcg gactgtgttg atctttctaa aaaagaggag aaaccgagtt
     5521 ggagcattgt ctcttcaaca ggatatattt acgaaaatct tgctaaatgt tagggtggcg
     5581 tctccgatca ttggaagacg catatatgaa attgcggcga ttagtagtgc tgccattcct
     5641 gggcgctgtc ggagttgcca tgcttaacgt gagcagagat caggccctca gcaatgcgct
     5701 ttcttgggga cctgtgtgga tacttcttgc cttgggggta tggtgggcat tagcaccgta
     5761 tatacgacca catcttgcac cgttctttgt ggcacgagcg ggcatgatgg tgatagtggc
     5821 ccttctggct atcatcactg tttcagcgat tgagacatat cgtgaagtac gtcctctgcc
     5881 atcgaaccct gggatagata tcacggaagc acctcctgga gtgtttccag acgtgccaga
     5941 aatgtttttg atcgatttcg gctcaaatgt cagcaaagtt gatcgacggc ttcttgagtg
     6001 gcaggtgccg ttcgaggata tgtcttttct aagatcattt cacggtttac tacgtggacg
     6061 aattccactt caaattttct ttgatgagca cggggcactc aaaattaatg cgacgatcta
     6121 tggtagtaat agagaaaagg tcgcggaaat tgagaacagt ttcttaaaga tccgtaatcc
     6181 caagtgggac tataatagcg attcgacggc atttgaaata gtcgatgcag atggaattcc
     6241 ctatttccag gtttatcgat acgaagcaaa cgcgattgca atacgtggtg tcttttatac
     6301 gtcggctggc gagcgcataa taagcaccga tcgaacagtt attttcgacg acaagaacgc
     6361 ggtgatgcgc cctcaaccat tatttctata tccccgccgg tttcactggg gtgaacgagt
     6421 cagacggcca tttatcgtcc tcgatgtcga ccgacagaaa tctatgagtc cggttgattc
     6481 aagaccaaat cgccaaatgt aaatagtcgt tccgtaattc cagtctccat cgctggtgtt
     6541 gagcgaatat agtcgtttac aagacaataa gcacagctgg attaaaatgg tccgcccaca
     6601 cgaaaaacca tgcaagcacc aaaatcttcg acaagagatt ttggacggtc aaagaagtga
     6661 cgacttccaa tgtattagct gccaaacttc ctttccacgt gattcccccg tagtccaatg
     6721 ctggcagcga cagcttagat caacacgcaa ataggggatt cccctgcgcg tcacacccac
     6781 cagaggccta gccatccgaa caatcccgat gtcgccaaga ccttctggaa accggtcaat
     6841 cggcgcgtgg tgagaatcac ggggaggctc agcggcgacg tgagaaagca ggcggtgaaa
     6901 aagacccacg tgggcagccg ttgcgcgcgg ggcgggtcgg gaaaaatcga acagaacggg
     6961 cattcgatct ggccgtccag cacctggccg caccgcgcgc aacgggtgcg gccgagcagt
     7021 tcgtcgaggt cctgctcttg ctcgctcatt ccttactgac ggggtctggc cgcttccggc
     7081 acgatagtca ccgcgagtcg ctcggcgcct cgaatcacgg tcaccggcga tggcacgcca
     7141 acggcggtgt ccgccaacaa tcgatggagc gcgtcgatcc cgtcaaccgg ccgatcgccg
     7201 aaggcgatga gcacgtcgcc cggcagaatg cccgcgcgtt cggccgggct cccgggctcc
     7261 acggacgcga ccaggatgcc gcgttccacc gcgaggccgt gaaagcggac cagtcggcgg
     7321 tgcaggggcg tgtcctgccc cgccagaccg aggtagccgc gggtaatgcg gccgtccttg
     7381 atcaaccgcc cagccacgaa cgccgcggtg ttgatcccga tcgcgaaaca caagccttgc
     7441 gccccacgga tcacggcggt gttcacgccg atcacccgtc cgcggctgtc cacgagcggg
     7501 cctccggagg tgccgggatt gagcgccgcg tcggtttgga gcacgttgtc gatgaggcgt
     7561 ccggaggttg agcgcagcga gcggccgagc gcgctgacca cgcctgccgt caccgtgcac
     7621 tgaaagccgt acgggttgcc gatcgcgatc gccagttgcc ccacgcgcac cgcgtgcgaa
     7681 tccccgaacg tcacggcggg gagccccggc gcgccgacgc ggatcacggc caggtccgtg
     7741 tccggatcgt cgccgaccag gtccgcctgc gcgcgccggc cgtcgaccag cgtgacggtc
     7801 agttggtcgg cgccgcgcac cacgtggctg ttggtcacaa tgaatccgtc gggggtgaac
     7861 aagaatcccg atccgctgcc ctgggcctcg aacgcgcggc gtctcgcgga acccgccacg
     7921 tggcggtgca cgtcgatatt cacgaccgag gggctcaccc gatccgccgc gcccgtgacc
     7981 gcgcgcgagt acgcgtcgag gagctcctcg tcggacgcgg catcaggcgt gcgttcctgg
     8041 cccggcgtta ttccatcaaa ggaaaccagt tcgatgaggt tgttcatggc cgattccatt
     8101 aaccgctccg ggggaggtgc cccctgtcct tcgacaagct caggacgaat ggaacatcaa
     8161 atctttcgtc cgtggtgagc cgtttgaaaa ctccgttcgt ggtgagcccg tcgaaccacg
     8221 ctcgattgaa taccggaccg ctcaccaccc tcgcaagtgg gagttcatct cccacaccaa
     8281 cacctcggcc ccgccggccg cgtcagccgt gaacctgcgc gcccccgccg ccaccaggcg
     8341 caccgaatcg ccggcctcga gccgcccgac gccttccagt tccgccgcgc ctttgggcac
     8401 gaacacgtgg atgaaccgcg cgtcgggtat ggtcacggtt tccccgggct tgagtcgccc
     8461 cacccacagc accgcatcct tctgccggat tgaaattgcc gcgtcatgcc cgcgtccgga
     8521 cgccagcggc accaagccgc ccttggcgat ctgcgggttg atgtccaact gctcgtacgc
     8581 gggattgatc cgctccgtgt ccgggattac ccacatctgc acgaagtgca cgggcgcatc
     8641 gccgctggga ttcatctcag agtgccagat tcccgtgccc gcgctcatgc gctgcgcgag
     8701 cccgggataa atgatgccct tgttgcccaa tgtgtccttg tgctccagct cgccgtccag
     8761 tacccacgtc acgatttcca tgtcctggtg cgggtgggtg cgaaacccgg tgcccggtct
     8821 gaccacgtct tcgttgttca cgagcagcag accgtggtgc gtgttcttcg ggtcgtagtg
     8881 cggcccgaag ctgaaactgt gtttgccgtg cagccagtcg atcgacgtga caaatcgatc
     8941 ggcgctgcgc tgaatgtggg tcaaggtggc aggcatgggc tcccccccga tcacgaggtc
     9001 gtgattcgac tctacgacga ccggaggggc ttcgtaaagg tcggggccgg ttacgcgatg
     9061 ttgatgacga agtgcatcgc aaagtccagc gcggtgccga gcacgcccac caggaacaag
     9121 ggaccgacga accgcggtcc ggtgcgccag aagtagacgg tgttgaacac cagcaacgcg
     9181 tcgtacccga tgttgacgat ctgatgcgcg agggggaacg ggcgcgcgag gaacgcggtg
     9241 gcgaagatga ggccgagcgg gaggatgacg agcagcaggg ggagcggctt ccgacgtgtg
     9301 atcaggacca ccaccaacgc agccacgagc gccatgaaca cgaccagaaa gaagacatgc
     9361 gcgaggaaga aggtctgggc gccggcgcgc tcggcctcgt gcatcgtcta ggactttatc
     9421 gtgccccagc gcagctggag cctgttcagg aatgagtcag atgccccctt cggggacttc
     9481 ctccgtgtcc attcttggcg gtgggtcgca aggcagcgcc gcgagaaccg aaccggagcg
     9541 tacaggtccc gtacgtgagc atcggaagcg cagcggcaac gcagcagatg accattcatg
     9601 gacaggctcc tagaaccagg ccctggcgag caacacgccg agttgcacgg cgatgaggcc
     9661 cgcgaacacg ctcagcgcca tgtagagcgc ggccgtgagc caacggccct ccaggaacag
     9721 gaggtggttc tcccacgcaa atgaggagaa cgtggtgtag gccccgagaa acccgatcgc
     9781 caacagccac tggacgtgct cgggtctggc gaccagttta tccacgatca cggttcccag
     9841 gatcccgatc agcaaacagc ccgagacatt aaccacgaac gtgcccaacg gcacgcgcga
     9901 gcccaaggtc gaagtgaccc acgtgcccac gataaatcgc gcattggcgc cgagaaaacc
     9961 gccgataccg accagcagat aggggatgag ttggcgcata ggttcctcgg atcatcttgt
    10021 ccggttgaaa ccggactacc ttaaaacaaa tgccagccgg ggtccagacc gaattatcta
    10081 cagggcgagg gggcgtcttg acttgaaggg ccgatggttt atagtggcgc gcgtgccgca
    10141 tcccgaaaag gagctctgat ggcgtaccgg ttgatctggc ttatcgtgat gatcgcaccg
    10201 tggcttctcg acgcgccggc gcgggcaatc gagtacgg
//
LOCUS       DBNGTT010000174         1648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_163593, whole genome shotgun sequence.
ACCESSION   DBNGTT010000174 DBNGTT010000000
VERSION     DBNGTT010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_163593"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC16BC_03990"
     CDS             <1..278
                     /locus_tag="AC16BC_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013879913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HIR3240809.1"
                     /translation="DGKIVGPGDSYAQTKQALKNIAAALEKAGARVNHVVRTRIFATD
                     ITAWEQIGKAHGEVFGAIRPATTMVEVKRLISPEMVVEIEADAVIEG"
     gene            298..840
                     /gene="yjjX"
                     /locus_tag="AC16BC_03995"
     CDS             298..840
                     /gene="yjjX"
                     /locus_tag="AC16BC_03995"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0046128 - purine ribonucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HIR3240810.1"
                     /translation="MSIAPLIVHVGSTNPVKIDAVREVFSESGRFPAFTLVPRDVLTG
                     VPEQPTSLDDTLCGAINRARNSFEACDLSIGIEDGLFAVPHTQSGYMNVCACAIFDGR
                     RDHLGLASAFEYPLEVVRLVIDEGLDVTQAFVKAGLSNNTKLGAAEGAIGVLTDGRLT
                     RRAYAKQAVIMALIHLPHTP"
     gene            882..>1648
                     /locus_tag="AC16BC_04000"
     CDS             882..>1648
                     /locus_tag="AC16BC_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3240811.1"
                     /translation="MTIAQPPLIPRTVLFGNPVRTSPQISPDGTRLAFLAPVHDVLNV
                     WVGSNDGTDAQPVTDDRDRGIRFYLWAADGQHILYLQDIGGDENWRLYAVHVDTKVVR
                     DLTPFADVQVQIVDRDKHFPHELLIAMNKEDARYHDVYRLDVRTGEMRIVAKNPGNVA
                     SWVTDARFDVRGALKATPDGGFELLLRDSPETTWRTLLTWSADDSLNSQPVGFSKDGA
                     AMYLEDASGSDTNQLVELRLTDGSRRALAHDPQFDIGG"
BASE COUNT          314 a          533 c          531 g          270 t
ORIGIN      
        1 cggacggcaa aatcgtggga cccggagatt cctacgcgca gaccaaacag gcgctcaaaa
       61 acatcgcggc ggcgttggag aaagcgggcg cgcgcgtgaa ccacgtggtg cgcacgcgga
      121 tctttgcgac cgacatcacc gcgtgggaac agatcggcaa agcccacggc gaggtattcg
      181 gagccatccg acccgccacc accatggtcg aagtcaaacg cctcatctcc ccggagatgg
      241 tggtggaaat cgaagcggac gcggtcatcg agggttgacc cgccgcgatc gatcggcatg
      301 tccatcgctc cgctcattgt ccacgtcggc tccacgaacc cggtgaagat cgacgcggtc
      361 cgcgaggtct tctccgagtc cggacgtttt cccgcgttca ccctggtccc gcgcgacgtg
      421 ctcacgggcg ttcccgagca gcccacgtca ttggacgaca cgctgtgcgg cgccatcaac
      481 cgcgcgcgca attcgtttga agcgtgcgat ctgagcatcg gcattgaaga cggcctcttc
      541 gcggtcccgc acacgcagag cgggtatatg aacgtgtgcg cgtgcgcgat ttttgacggc
      601 cgtcgcgacc acctcgggct ggcctcggcg ttcgagtatc cgctggaggt ggtgcggctg
      661 gtgatcgacg aaggcctgga cgtgacccag gcgttcgtga aggcggggtt gagcaacaat
      721 acgaagctgg gcgcggcgga aggcgcgatc ggggtgttga ccgacggccg cctcacgcgc
      781 cgcgcatacg ccaaacaagc cgtgattatg gcattgatcc atttgccgca caccccgtag
      841 gggcacggca tgccgtgccc ctacactacc atccgacgcc aatgacgatc gcccaaccgc
      901 ccctgatccc gcgcaccgtg ttgttcggca atccggtgcg aaccagcccg caaatttcgc
      961 ccgacggcac gcgcctcgcg tttctcgcgc cggtgcatga cgtgctgaac gtgtgggtcg
     1021 gttcgaacga tgggaccgac gcgcaaccgg tgacggacga ccgcgaccgc ggcatccggt
     1081 tctatctgtg ggccgcggac ggccaacaca ttctctacct gcaggacatc ggcggcgatg
     1141 agaactggcg gctctatgcg gtccacgtgg acaccaaggt cgtccgcgac ctcaccccgt
     1201 tcgccgatgt gcaggtccag atcgttgacc gcgataaaca cttcccgcac gagctgctga
     1261 tcgccatgaa caaggaggac gcgcggtacc acgacgtgta ccgtctggat gtgcggacgg
     1321 gtgaaatgcg gatagtggcc aagaaccccg gcaacgtggc atcgtgggtc actgacgcgc
     1381 ggtttgacgt gcgcggcgcg ctcaaggcca cgccggacgg agggttcgag ctattgcttc
     1441 gtgactcccc ggagacaacc tggcgaacgc ttctcacctg gagcgcggac gacagcctca
     1501 acagccaacc cgtgggcttc tcgaaagacg gcgcggcgat gtatctggaa gacgccagcg
     1561 ggagtgacac gaaccagttg gtcgagctgc gactgaccga cggctcgcgc cgggccctgg
     1621 cccacgatcc acagttcgac atcggcgg
//
LOCUS       DBNGTT010000175         2877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1057534, whole genome shotgun sequence.
ACCESSION   DBNGTT010000175 DBNGTT010000000
VERSION     DBNGTT010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1057534"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="AC16BC_04005"
     CDS             <1..1003
                     /locus_tag="AC16BC_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3240812.1"
                     /translation="VLEGVRKAYPGRVIYERLDLIVTRGQRVALVGPNGAGKSTLLKM
                     LAGVLTPDAGTVEYGQNVTVAYYAQHQLEALDPKATVLDEMISAAPDQELPFLRGILG
                     AFLFSGDEVKKRVAVLSGGEKSRLALAKMLVRPANFLLLDEPTNHLDIPSRDVLESAL
                     LEYTGTICFITHDRHFIQSVANTVLDVRDGTAVVYPDGYEYYLEKKARQEAESAAQAI
                     APPPEEEPRVGEPERTGFKSKEQRRRDAEERARRSAQTKPVKTRLDRIERELAEKGKE
                     LERLTAMLADPATYEDKAKFFSTMDDHARLKKAVDELTAEWGDLQEQYEALVNAVDA"
     gene            complement(1019..1423)
                     /locus_tag="AC16BC_04010"
     CDS             complement(1019..1423)
                     /locus_tag="AC16BC_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3240813.1"
                     /translation="MASPLHARLHHVAVQTPDLARARRFYETVMGFTVLKTERTAKGR
                     DIVWLACGDCRIELYGAKPGQTLSAGWTTDAVGPLALGFLVDDLDAEVERLRTLGIPI
                     TKPPYEPVPGERAAMIAGPDGEEIVLLEQPVG"
     gene            1490..2020
                     /locus_tag="AC16BC_04015"
     CDS             1490..2020
                     /locus_tag="AC16BC_04015"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3240814.1"
                     /translation="MRALFRLIVWLAGLALVVLIASKVLLEPTYVVSTQVVIHVAPGK
                     VWEKVGALDAWPAWVDGLQRLSVVKGGGREVGSLADVHVYNGFQGWDMTIQIVETVPD
                     HRLRYQVLGGPQNGVQSTIELTPSQDARSTTVSWNESDTPGGLWGNLLAVGMKLIVET
                     QHDASLNQLKFALERA"
     gene            2027..2641
                     /locus_tag="AC16BC_04020"
     CDS             2027..2641
                     /locus_tag="AC16BC_04020"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR3240815.1"
                     /translation="MTDRELQAARRHLKRADAVLREVIEQVGNYQIRYRDDRFLALVE
                     AIVAQQVSVGAARAILKRLLDQHPKGLTAERLMTTPPESLRAVGLSRQKTDYLIGLAR
                     HVEEGTFDLASIHGLDDEQVAKTLTQVKGIGRWTVEMFLIFCLRRPDVLPALDLGFRK
                     AVQKAYRLRELPAPDKIEALAVPWQPYRSVATCYLWASLEPEPW"
BASE COUNT          543 a          822 c          988 g          524 t
ORIGIN      
        1 ggtgctcgaa ggcgtgcgca aggcctatcc gggtcgcgtg atctatgagc gattggatct
       61 gatcgtcacg aggggccagc gggtcgcgct ggtgggaccc aacggcgctg gcaaatccac
      121 tctcctcaag atgctggccg gcgtgttaac accggacgcg gggaccgtgg agtacggcca
      181 gaacgtcacc gtggcctatt acgcgcagca ccagctcgaa gcgctcgacc ccaaggccac
      241 ggtgttggac gagatgatct cggccgcgcc ggatcaagaa ctgccgtttc tgcgcgggat
      301 tcttggcgcg tttttatttt ccggcgacga ggtcaagaag cgcgtggccg tgctctccgg
      361 cggggagaag agccgtctcg cgttggccaa gatgctggtg cgtccggcca atttcctgct
      421 gctggacgag cccacgaacc atctggacat tccttcgcgc gacgtgctgg agtccgcgtt
      481 gttggagtac accggcacga tctgcttcat cacgcacgat cgccatttca ttcagtccgt
      541 ggccaacacg gtgttggacg tgcgcgacgg gactgcggtc gtgtatccgg acggatacga
      601 gtattacctg gagaaaaagg cgcggcagga agcggaatcc gcggcgcagg cgatcgcgcc
      661 gcccccggag gaggaaccgc gggtcggcga acccgaacgg accgggttca agtcaaagga
      721 acagcgccgg cgggacgcgg aggagcgcgc caggcggtcc gcgcagacca agccggtcaa
      781 gacgcgtctg gacaggatcg agcgcgaact cgcggagaag ggcaaggagc tggagcggct
      841 caccgcgatg ctggcggacc ccgcgacgta cgaagacaag gcgaagtttt tttcaaccat
      901 ggacgatcac gcgcgtctga agaaggccgt ggatgaattg accgcggagt ggggagacct
      961 tcaggagcag tacgaggcgt tggtcaatgc ggttgacgcc tgagccagag gtggtcggtt
     1021 acccgaccgg ctgttcgagt aggacgattt cttcgccgtc ggggcccgcg atcatggcgg
     1081 cgcgttcacc cggcacgggt tcgtacggcg gtttggtgat cgggatgccc agggtgcgca
     1141 gccgctcaac ttcggcatcg aggtcgtcga ccaggaatcc gagggcgagc gggccgaccg
     1201 catcggtcgt ccatccggcg gacagggttt ggcctggctt ggcgccgtac agttcgatgc
     1261 gacagtcgcc gcaggcgagc cagacgatgt cgcgtccctt ggccgtgcgt tcggttttca
     1321 ggaccgtgaa gcccatcacg gtctcataga agcgacgagc gcgagccaag tccggggttt
     1381 gcacggccac gtggtgcagc cgggcgtgga gtggtgacgc catgcgcgca gtatatcaga
     1441 gtccgatggg cggatcagcg ggatcgccct gacagtagga ggaaatacga tgcgcgcgtt
     1501 gttcaggctg atcgtgtggc tcgccgggtt ggcgctggtg gtgttgatcg cctccaaggt
     1561 tctgctggag cccacctatg tcgtctcaac gcaggtagtg attcatgtcg cgcccgggaa
     1621 agtctgggag aaggtcgggg cgctggacgc gtggccggca tgggtggacg ggctgcagcg
     1681 gctcagcgtg gtgaagggcg gggggcggga ggtcgggagc ttggccgacg tccatgtcta
     1741 caacggcttt cagggatggg acatgacgat tcagatcgtc gagaccgtcc ccgatcatcg
     1801 cctccggtat caggtgctgg gcggacccca gaacggcgtc cagtcgacga tcgaactcac
     1861 tccttcgcag gatgcccgat caaccacggt aagttggaac gaatccgata ccccgggtgg
     1921 actctggggc aatctgctgg ccgtgggcat gaagttgatc gtcgaaaccc aacacgatgc
     1981 gagtctcaat cagctcaagt ttgcgctgga gcgcgcttag cccggcatga ccgaccggga
     2041 actgcaagcc gccaggcgtc acctgaaacg ggctgacgcg gtgttgcgcg aggtaatcga
     2101 gcaagtcggg aactaccaga tccggtaccg cgacgaccgg tttctcgctt tggtcgaagc
     2161 catcgttgcc caacaagtgt cggtcggcgc cgcccgcgcc attctcaagc gactcctcga
     2221 tcagcacccc aaaggtctca cggccgaacg attgatgacg acaccaccgg aatcgctgcg
     2281 tgccgtgggt ctctctcgtc aaaaaaccga ctacctgatt ggcctggccc gccatgtgga
     2341 agaaggcacg tttgatctgg ccagtatcca tgggttggac gatgagcagg tcgccaagac
     2401 gctgactcag gtcaaaggca tcggtcgctg gaccgtggag atgtttctga tcttctgtct
     2461 gcgccgcccc gacgtcttgc cggcgctcga tctggggttt cggaaagccg ttcagaaagc
     2521 gtaccgactc agagagctcc cggcgcccga caagatcgaa gcgctcgcgg tcccctggca
     2581 gccgtatcga tcagtggcca cgtgttatct gtgggcgagc ctggagccgg agccgtggta
     2641 agaacgtctc gaaaccacga gcgagcatcc ggcggaatcg ttaaattgcg gcggatgtcg
     2701 tggcgcccgg tactgactct acagcgccgt tgacaggccc aggaggtaca agcgttctcg
     2761 gaactcatcg cgcgggatat tgggatcctt cgggatggtg ctgtcttttc gaaggaactg
     2821 aacctggagg atcaacgccc agcgggtggt ccagtctttt cggagcgagg tgtcggc
//
LOCUS       DBNGTT010000176         4056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1185391, whole genome shotgun sequence.
ACCESSION   DBNGTT010000176 DBNGTT010000000
VERSION     DBNGTT010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1185391"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1224)
                     /locus_tag="AC16BC_04025"
     CDS             complement(91..1224)
                     /locus_tag="AC16BC_04025"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009566821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3240816.1"
                     /translation="MKILIVGGGTGGTILANNLARRLKAEMKTGKARITMLSASDKHM
                     YQPGLLYVAFGRMTTDELYRDQASLLEPGIDFQVDPVEEFSLDKNQVKTKSGKTIGYD
                     LVVIATGSRAVPEEIPGFAEHAIHVYTEEAAVKFYQTINKFQGGRVVIAVGVPHKCPM
                     IPPEVTFCLYDYFKDRNMLDKVQFHYTYPIGRLHSLEPVSKWMEGEFAQRGITSETLF
                     NIKEIDGARKVVRSEEGTETPFDLLVAVPAHKGMQVIEKNSLGKGGWVPVNKFRLNID
                     GRHNEFAIGDTTNIPISKAGSTAHFEAEALAENLASIVKTGEPVRDYDGKVFCFIEAG
                     KDKATYAMFNYTMPPDPKPPTKSLHWFKMAYNQMYWASARGLL"
     gene            complement(1272..1502)
                     /locus_tag="AC16BC_04030"
     CDS             complement(1272..1502)
                     /locus_tag="AC16BC_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR3240817.1"
                     /translation="MAEKQMIDARGAFCPGPLMELIANIKLVNVGDELEVLSTDKGSA
                     TDIPAWVKKVGHQLVSSSEDAGVWHIVVRKAK"
     gene            complement(1517..1912)
                     /locus_tag="AC16BC_04035"
     CDS             complement(1517..1912)
                     /locus_tag="AC16BC_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR3240818.1"
                     /translation="MSDTQKLSVIVVSGSHERLQMAAMVVSVAAVSGDQVSVFLSMNA
                     LGHFRKGQAAAPTPEGDFGRVMAQKKVPKFKDLFAQAVELGDAKIYPCSMAMDVLALQ
                     PGDLEPYLAEPIGLTAFLIDAAGSQAWTF"
     gene            complement(1905..2048)
                     /locus_tag="AC16BC_04040"
     CDS             complement(1905..2048)
                     /locus_tag="AC16BC_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240819.1"
                     /translation="MSSEPLPAPTVPYVARRCRDLKGFRHLVVGSFDRGAEPSFTKAR
                     GHE"
     gene            complement(2157..2414)
                     /locus_tag="AC16BC_04045"
     CDS             complement(2157..2414)
                     /locus_tag="AC16BC_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3240820.1"
                     /translation="MKVLIPSPLLSYTNEREVEAAGATIADLLVDLDRRYPGLRFRVI
                     DEQDRMRAHMRFFVNGEQVFDLAHALRPTDGVQIVQALSGG"
     gene            complement(2463..3665)
                     /locus_tag="AC16BC_04050"
     CDS             complement(2463..3665)
                     /locus_tag="AC16BC_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3240821.1"
                     /translation="MARTIKKSATKTTRKTARSTRSVRRPSRKTLVLLATRKGAWLLR
                     GDAARKTWRVDGPHFLGHIINHLVLDPRDGRTLLAAAKTGHLGPTIFRSTDFGRTWKE
                     AAKPPAFAKAPEGEKGRAVDHTFWLAPCHANEPNAWYAGTSPQGLFRSEDGGVTWEPF
                     SYINDDPQYRVWMGSVQDGTQDGPKMHSIIVDPRDPAHLYFAMSGGGVHESVDRGRTF
                     TPLVNGMEVVAGFDASNIAFHDPHCIRLCPSNPDRLYQQNHCGIYRIDRPSNEWVRIG
                     KNMPKQVGDIGFPMVVHPRDASTVWVFPMDGQTVWPRTSPDGKPAAFVTRNAGKTWQR
                     LDSGFPRSQAWWTVKRQAMTADAQDPVGLYFGTTSGELWASRDEGKRWARIAGNLPEI
                     YAVEAAEV"
     gene            complement(3724..4056)
                     /locus_tag="AC16BC_04055"
     CDS             complement(3724..4056)
                     /locus_tag="AC16BC_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3240822.1"
                     /translation="MTESSPPSKVTMALNMLSPFEAHNIVEFTLALGIGLAELTCVVL
                     YVIPRTAVLGAILLTGYLGGATASHVRIGEPFFMAIVMGVLVWGGLYLRDDRLRALLP
                     LRKEPAQR"
BASE COUNT          703 a         1329 c         1250 g          774 t
ORIGIN      
        1 tctggaccgc tccaaggggg cggttgggaa ccccctccct ccccgcagat cgcgaagcaa
       61 cgccgcagat ggactctttc agcagcccgc ctacagcagc ccgcgcgcgc tggcccaata
      121 catttgattg taggccatct tgaaccagtg cagcgacttc gtcggcggct tgggatcggg
      181 cggcatggtg tagttgaaca tggcgtacgt ggccttgtcc ttacccgctt cgatgaagca
      241 gaagaccttc ccgtcgtagt cgcggaccgg ctcgccggtc ttgacgatcg aggccaggtt
      301 ctccgccagc gcctcggctt cgaagtgtgc ggtggagccg gccttgctga tcgggatgtt
      361 ggtggtgtcg ccgatggcaa attcgttgtg ccgaccatcg atattcaacc ggaacttgtt
      421 caccgggacc cagccgcctt tgccgaggct gtttttctcg atgacctgca tgcccttgtg
      481 cgcggggacg gccaccaaca ggtcgaacgg cgtctccgtg ccctcctcgc tgcgcaccac
      541 cttcctcgcg ccgtcgatct ccttgatgtt gaacaaggtc tccgacgtaa tgccgcgctg
      601 cgcgaactcc ccctccatcc acttgctcac gggttccagg ctgtgcagcc gaccgatcgg
      661 gtaggtgtaa tggaattgca ccttgtcgag catgttgcgg tctttgaagt agtcgtagag
      721 acagaacgtg acctcgggcg ggatcatcgg acacttgtgc ggcacgccta ccgcgatcac
      781 cacccgccca ccctggaact tgttgatggt ctggtaaaac ttgaccgcgg cttcctcggt
      841 atagacgtgg atcgcgtgtt ccgcgaagcc ggggatttcc tcgggcacag cccgagaccc
      901 ggtcgcgatc acgaccaagt cgtacccgat ggttttcccg ctcttggtct tgacctggtt
      961 tttgtccagg gaaaactcct cgaccgggtc gacctgaaag tcgatgcccg gctcgagcag
     1021 gctcgcctgg tcccgataca gctcgtccgt ggtcatgcgc ccgaacgcca cgtacagcaa
     1081 accgggttgg tacatgtgtt tgtccgacgc ggacagcatg gtaatgcgcg ccttgccggt
     1141 cttcatttcg gccttgaggc gccgggccag attgttcgcc aggatcgtgc cgccggttcc
     1201 gccgcccacg atgagaatct tcatgccact gctcctttca tcacccgggg aatttcagtc
     1261 gaatcgatcg gttacttggc tttgcgcacc acgatgtgcc agacccccgc gtcctctgac
     1321 gacgagacga gctgatgccc cacctttttg acccacgcgg ggatgtcggt cgcggatccc
     1381 ttgtccgtgg acagcacttc cagctcatcg cccacgttca ccagcttgat gttggcgatc
     1441 agctccatga gcgggcccgg gcaaaacgcg ccgcgtgcat cgatcatctg tttctcggcc
     1501 atcgggattc ctcgcctcag aacgtccagg cttggctgcc ggccgcgtcg atcaggaacg
     1561 cggtcaaacc gatcggctcc gccagatacg gttccaggtc gcctggctgc agggcgagca
     1621 cgtccattgc catggaacag gggtagatct ttgcgtcgcc caactcaacc gcctgggcaa
     1681 acaggtcctt gaacttgggc accttctttt gcgccatgac gcgcccgaaa tcgccttccg
     1741 gcgttggggc ggcggcctgg cctttgcgaa aatggcccag ggcgttcatg gacaagaaca
     1801 cgctgacctg gtcgccgctc accgcggcca ccgacaccac catcgcggcc atttgcagac
     1861 gctcgtgcga accggagacc acgatcacgc tgagtttttg cgtatcactc atgcccgcgc
     1921 gccttggtga aagacggctc ggccccgcgg tcgaacgatc cgaccaccag gtggcggaac
     1981 cccttcagat cccgacatcg cctcgccacg tacggaacag tcggagccgg aagcggctcc
     2041 gaagacatgc gtctgcatcc tagcgaagag cgttggcggg tgtcaacaaa cggcccagcg
     2101 acggcgttac cggctcggat cagcgccgcc gagcctcgcg ctgaacagac cgcgcctcac
     2161 ccgccgctca acgcctgcac gatctgcacc ccatcggttg ggcgcagcgc gtgcgccaga
     2221 tcgaacactt gctcaccgtt gacgaagaac cgcatgtgcg cccgcatccg gtcctgctca
     2281 tcgatcacac ggaaccgcag gccggggtat cggcgatcca ggtcaacgag caggtccgcg
     2341 atcgtggcgc cggcggcctc gacttcgcgc tcgttcgtgt aggagagcaa cggactgggg
     2401 atcagcacct tcatgggcct gacttcaata cgacgatatg cgtcggcaca acgaccgcgt
     2461 gatcacacct ccgcggcttc caccgcgtag atttcgggga gatttcccgc gatgcgcgcc
     2521 catcgtttgc cttcgtcgcg gctcgcccac aactccccgc tggtggtccc gaagtacagg
     2581 cccacgggat cctgtgcgtc cgcggtcatg gcctggcgct tcacggtcca ccaagcctga
     2641 ctacggggaa aaccggaatc caaacgctgc caggtcttgc cggcgttgcg cgtgacaaac
     2701 gcggcgggct ttccgtccgg gctggtgcgc ggccacacgg tctggccgtc catagggaac
     2761 acccaaacgg tgcttgcgtc gcgcgggtgc accaccatgg ggaacccaat gtcgccgacc
     2821 tgcttcggca tgttcttgcc gatgcgaacc cactcgttgg atgggcgatc gatccgatag
     2881 atgccgcagt gattttgctg gtacagccgg tccgggttag aaggacagag ccggatgcaa
     2941 tgggggtcgt ggaacgcgat attgctcgcg tcaaagcccg cgaccacttc catgccgttg
     3001 accaggggcg tgaaggttcg gccgcggtca acggattcgt gcacgccgcc ccccgacatg
     3061 gcgaagtaga ggtgggcggg atcgcgcggg tccacgatga tcgagtgcat tttcgggccg
     3121 tcctgtgtgc cgtcctgcac gctgcccatc cacacgcggt actgcgggtc gtcgttgatg
     3181 taggaaaacg gctcccacgt cacgccgccg tcttcggagc gaaacaggcc ctgcggcgag
     3241 gtgcccgcgt accacgcgtt gggctcgttc gcgtggcagg gcgcgagcca gaacgtgtga
     3301 tccaccgcac ggcctttctc cccctcagga gctttggcga acgcgggcgg cttggcggct
     3361 tccttccagg tgcggccgaa atccgtggaa cgaaagatgg tcggaccgag gtggccggtc
     3421 ttggccgcgg ccaggagcgt gcgaccgtcc cgagggtcga gcaccaggtg gttgatgatg
     3481 tggccgagaa agtgcggccc atccacccgc cacgtcttgc gcgccgcgtc cccgcgcagc
     3541 agccacgcgc ctttgcgcgt ggccaggagg accagcgtct tacgtgacgg gcgacggacc
     3601 gaccgcgtgc tgcgagcggt ttttcgagtt gttttggttg cggacttctt gatcgtgcgc
     3661 gccataaatc gctccctcta cgtgaccacg tgaaattcgc cgtccatcac agccggaata
     3721 tttttaccgc tgcgcgggct cttttcgcaa cggcaggagc gcgcgcagcc ggtcatcgcg
     3781 caggtacaat ccgccccaga ccagcacgcc catcacgatc gccatgaaga acggctcgcc
     3841 gatccgcacg tggctcgcgg tggcgccgcc cagatacccg gtgagcaaga tcgcgccgag
     3901 caccgcggtg cgcggaatca cgtagagcac cacacaggtc agttccgcga gcccgatgcc
     3961 gagcgcgagc gtgaactcga cgatgttgtg ggcctcgaac ggcgagagca tgttcagcgc
     4021 catcgtgacc ttggacggag gcgaggactc ggtgat
//
LOCUS       DBNGTT010000177         3957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1441206, whole genome shotgun sequence.
ACCESSION   DBNGTT010000177 DBNGTT010000000
VERSION     DBNGTT010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1441206"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="AC16BC_04060"
     CDS             <1..1174
                     /locus_tag="AC16BC_04060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3240823.1"
                     /translation="PDTFLSYNYLCHFAVVRKRILDDLGGFREGYDGAQDYDLLLRVV
                     EKTQAIRRIPRVLYHWRAAAASTAAGVKVKPYAVSAAKRAIAEHLARRGLDAEVLDGD
                     ALGSYRVKYRVLPGQRVCIIVPTRDKVHLLERCVSSVLAKTSHDLFTILIVDNRSREP
                     ETLAYFDRIKEHPKVRVARYDQPFNFSAINNFAVRGTDSDYLVFLNNDTEVIGPEWLD
                     AMLEFAQRDDVGAVGAKLYYPNDTIQHAGTIVGYWGVADHAHKHYARSARGYMGRINI
                     IQNLTAVTAACMMMRRKVFDEVGGYDEAFSHSFNDVDLCLRIRDKGYLVVYTPYAELY
                     HHESVSRGHDEKPGERARLDREIALMKARWKRVFDAGDPYYSPNLTLEKTDFSFRI"
     gene            1215..2264
                     /locus_tag="AC16BC_04065"
     CDS             1215..2264
                     /locus_tag="AC16BC_04065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3240824.1"
                     /translation="MSVALCTYNGGRYLGEQLESLASQTLKPRELVVCDDGSTDDTVA
                     IIRDFALRARFPVRLFLNPANLGSTKNFEHAIQRCEGDLIALSDQDDVWRPRKLERVV
                     AAFEAAPDVGAVFSDGEVVDETLRPLGYGLWDAAGFDAARRERLGQGHALEVLLKNDV
                     VTGAALAFRRTFRDAIVPIPAIWVHDAWIALVIAALGKVGTIDEPLILYRKHRHQQVG
                     VVSRSDEGALAWIWSSLADVRRDVDGYVAIGDEQYTLFAERYEIGGERLAALGARVNP
                     RAIAGCRAKAAHFRARAAMTSHRWRRVPLVLGELARLRYFRYSSGAKSLARDLVRAAL
                     FWKGTVPRASGGETP"
     gene            2261..3208
                     /locus_tag="AC16BC_04070"
     CDS             2261..3208
                     /locus_tag="AC16BC_04070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3240825.1"
                     /translation="MSPNRAQPLVVIIVLNWNGRDDTVACVESVRRLRYGNARALIVD
                     NGSTDDSVAVFAQRFPDIRCIQTGANLGFAEGNNVGMRTALAEGADYVFLLNNDATIH
                     PDALDRLVAAGESRSDIGIVGPKIYYAADPKRIWYAGGTISPLGRLGHEGWNRIDDGR
                     WDAPRETGYVTGCAFLVKRGVLEKVGLFAPEYFLLFEEADLCVRARRAGFRCWVEPRA
                     IVRHKVSSSFGGFSPQYYYYFLRNYLLFQRRHVIPEKGWAARLAIASYLVRVFVYHLF
                     TPHRGGWRNLAAAWEGIRDALRGRWYQRAIHRLIGEDAA"
     gene            3266..3799
                     /gene="cysC"
                     /locus_tag="AC16BC_04075"
     CDS             3266..3799
                     /gene="cysC"
                     /locus_tag="AC16BC_04075"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR3240826.1"
                     /translation="MTSGFTIWFTGLSGAGKTTVAHRVAELLRQRLGRAVEILDGDVV
                     RTHLSKGLGFSREDRDTNIRRIGWVCYVLTKHGVPNIAAAISPYRGVRDEVRKMVGRF
                     VEVYAECPLAVCESRDVKGLYRKARAGELKQFTGIDDPYEEPLDPEVTLFTDQETVEE
                     STQKVIAAIEKLGWVPR"
BASE COUNT          663 a         1153 c         1418 g          723 t
ORIGIN      
        1 gccggacacg tttctgtcct ataactacct gtgtcatttc gcggtggtga ggaagcggat
       61 cctggatgat ctgggcgggt tccgcgaggg atacgacggg gcgcaggact acgacctgct
      121 gctgcgcgtg gtcgagaaga cgcaggcgat tcggcgcatc cccagggtgt tgtatcactg
      181 gcgggccgca gcggcatcga ccgcggccgg ggtgaaggtg aagccgtatg ccgtctccgc
      241 cgcgaaacgg gccatcgcgg agcacttggc tcgccgcggt ctggacgcgg aggtcctgga
      301 cggcgacgcg ctgggaagct accgcgtcaa atatcgcgtg ctgccggggc agcgcgtctg
      361 catcatcgtg cccaccagag acaaggtcca tttgttggaa cgatgcgtgt cgtccgtgct
      421 cgccaagacg agtcatgacc tcttcacgat cctgatcgtc gacaaccgga gtcgggagcc
      481 ggagacgttg gcgtactttg accgcatcaa ggagcacccc aaggtgcggg tggcgcggta
      541 tgaccaacct ttcaattttt ccgccatcaa taactttgcg gttcgcggca cggactcgga
      601 ctacctggtc tttctgaaca atgacaccga ggtgatcggc cccgagtggc tcgacgccat
      661 gttggaattc gcgcagcgcg acgacgtggg cgcggtcggc gccaagctgt actaccccaa
      721 tgacaccatc cagcacgccg ggaccatcgt gggctattgg ggcgtggcgg accacgcgca
      781 caagcattat gcccggtcgg cccgggggta catgggacgg atcaatatca ttcagaatct
      841 gaccgcggtc accgcggcgt gcatgatgat gcggcgaaag gtcttcgacg aggtgggcgg
      901 ttacgacgag gcgttctcgc attcgttcaa cgacgtggat ctctgcctga gaattcggga
      961 caagggctac ctggtcgtct acaccccgta cgccgagctc tatcaccacg agtccgtgag
     1021 ccggggacac gacgaaaaac cgggggagcg agcgcggctg gaccgggaga tcgcgttgat
     1081 gaaggcgcgc tggaagcgcg tctttgatgc cggcgatccg tattacagtc ccaacctcac
     1141 gctcgagaag acggatttta gcttcagaat ttaagagggc ggccggggcc gaacgggctg
     1201 ttcagggcgg gcgcgtgtcc gtcgcgctgt gtacgtacaa cggcggccgg tacctcgggg
     1261 aacagctcga gagcctcgcg tcgcaaacgc tcaagccccg cgagctggtg gtctgcgacg
     1321 acggctcgac cgacgacacg gtcgcgatca tccgggactt cgcgcttcgg gcgcggttcc
     1381 cggtccggct gttcctcaac ccggccaatc tcggttcgac caagaatttc gagcacgcca
     1441 tccaacgatg cgagggcgac ctgatcgcat tgtcggatca ggacgatgtg tggcggccgc
     1501 ggaaactgga acgggtggtg gcggcgttcg aggccgctcc ggacgtcgga gccgtgttca
     1561 gtgacggcga ggtcgtcgac gagaccttgc ggccgctcgg ctatgggctg tgggacgccg
     1621 cggggttcga cgcggcgcgt cgcgagcgtc tgggacaggg ccacgcgctc gaggtgttgc
     1681 tgaaaaacga cgtggtcacc ggcgccgccc tggcgttccg gcggacgttt cgggacgcga
     1741 tcgtgccgat tcccgcgatc tgggtgcacg acgcgtggat cgccttggtg atcgcggcgc
     1801 tgggcaaggt ggggaccatc gacgaacccc tcatcctgta ccgcaagcac cgccaccaac
     1861 aagtcggcgt ggtgtcgcgc tccgatgagg gcgcgctggc gtggatctgg tcgtcgctgg
     1921 ccgatgtgcg tcgcgacgtg gatgggtatg tggcgattgg agacgaacag tacaccctgt
     1981 tcgcggagcg ctacgagatc gggggcgagc ggctggcggc actcggtgcc cgggtgaatc
     2041 cgcgcgcgat cgcgggatgc cgggccaagg ccgcgcattt tcgcgcgcga gccgcgatga
     2101 cctcgcaccg ctggcgccgg gtgcccctgg tgctcggcga gctggcgcgt cttcgctatt
     2161 tccggtattc gagcggagcc aagagtctgg cgagggatct ggtccgcgcg gccttgttct
     2221 ggaaagggac ggtccctcgg gcgtccgggg gcgaaacccc gtgagcccga accgcgcgca
     2281 gcccctggtg gtgatcatcg tgctcaactg gaacgggcgc gacgacaccg tggcgtgcgt
     2341 ggagtcggtc cgtcgcctgc ggtatgggaa cgccagggcg ctgatcgttg acaacgggtc
     2401 cacggacgat tcggtcgcgg tgttcgcgca acggttcccg gacatccgct gcattcagac
     2461 cggcgcgaat ctgggatttg ccgagggaaa caacgtcggt atgcgcacgg cgctcgcgga
     2521 gggcgcggat tacgtgttcc tgctcaacaa cgacgcgacg atccatcccg atgcgctgga
     2581 tcggctggtc gcggcgggcg agtcgcggtc ggatatcggg atcgtggggc ccaagattta
     2641 ttatgcggcc gatccgaagc gaatttggta cgcgggcggc acgatcagcc cgctggggcg
     2701 gctggggcac gagggatgga accggatcga cgacggccgg tgggatgcgc cgcgcgagac
     2761 cgggtacgtg accggatgcg ccttcctggt caagcggggc gtgctggaga aggtcgggct
     2821 cttcgccccc gagtatttcc tgctctttga agaagccgat ctgtgcgtgc gcgcgcgacg
     2881 cgcggggttt cggtgctggg tcgagcctcg ggcgatcgtg cggcacaaag tttcgtcgtc
     2941 gttcggcggg ttttctccgc agtactacta ttactttctg cgcaactatc tgctctttca
     3001 acgccgtcat gtgattccgg agaagggatg ggccgcgcgg ctcgccattg cgagctacct
     3061 ggttcgcgtg tttgtctacc acctcttcac gccgcatcgc ggagggtggc ggaacctggc
     3121 cgcggcgtgg gagggcatcc gcgacgccct ccgtgggcgg tggtatcaac gagcgatcca
     3181 ccggctgatc ggggaggacg ccgcatgagc gtgagtcgac ttgtgcgtgt cgatgagagg
     3241 aaaaggaggg gctgtgggag agacaatcac tagcggtttt acgatctggt tcaccggact
     3301 ctccggcgcg ggcaagacca cggtggcgca tcgcgtggcg gagttgctgc ggcagcggct
     3361 cggtcgcgcg gtggagattc tggacgggga cgtggtccgc actcatctga gcaagggact
     3421 cgggttcagc cgcgaggatc gcgacaccaa tatccggcgg atcggctggg tctgctacgt
     3481 gttgaccaaa cacggggtgc ccaacatcgc ggccgcgatt tcgccgtatc gcggggtccg
     3541 ggacgaggtg cgtaagatgg tcgggcggtt cgtggaggtg tatgccgagt gtccgctcgc
     3601 ggtgtgcgag tcgcgcgacg tgaaggggtt gtaccgcaag gcgcgcgccg gcgaactcaa
     3661 gcagttcacg ggcatcgacg atccctacga agagcctctc gacccggaag tgacgctgtt
     3721 cacggatcaa gagaccgtgg aagagagcac tcagaaggtg atcgccgcga tcgagaaact
     3781 cgggtgggta ccgaggtagg cgcgtagcac ctcatgcggt cgcgatgcgg atcttgatcg
     3841 gttccatgtc gttcccgctg cctcccaagt ccggggggcg gttgcgcgtt ttcaacctgc
     3901 tcaaacacct ggcgtcgcgg catcgcgtca cgttgctcac gctcgcggat tcggggg
//
LOCUS       DBNGTT010000178         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3357486, whole genome shotgun sequence.
ACCESSION   DBNGTT010000178 DBNGTT010000000
VERSION     DBNGTT010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3357486"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="AC16BC_04080"
     CDS             <1..1109
                     /locus_tag="AC16BC_04080"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3240827.1"
                     /translation="FLAENAQFAEACEAAGFKFIGPSPESIALMGDKAKARELMIKYG
                     VPVIPGSTGALRDEAEARETAKRIGYPVIVKASAGGGGRGMRVVNQEDDLLKAIQTAQ
                     TEAKSAFGDDSVYLEKYFIDPRHIEVQILADKKGHTIALGERDCSIQRRHQKLIEESP
                     SPAVSPELRREMMKAAVDAVKAAKYVNTGTVEFLLDKHQRFYFIEMNTRIQVEHPVTE
                     MVVDMDLIKEQIMVAAGRSLAMKQRDVKLRGHAIECRINAEDPERFTPSPGTISVYHV
                     PGGPGVRVDSAAYPGCVIPPHYDSLVAKVIVTGETRLEAIARMKRALAEFQIEGIKTT
                     IPLQRRIMDDPNFVKGKFSTGFLERFLSADEPAA"
     gene            1270..1890
                     /gene="thiE"
                     /locus_tag="AC16BC_04085"
     CDS             1270..1890
                     /gene="thiE"
                     /locus_tag="AC16BC_04085"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3240828.1"
                     /translation="MFSSPLYWIADASLVGERSLVELAEAAIGSGLRAFQYRDKTRSP
                     RDQYPIALALADLAGRTGATFVINDDIDLALVVEAHGVHLGQEDFPVAEARRILGRKM
                     LIGCSVHTIDQAKAAEADGADYLGVGPVYESSTKMARPPLGCEQLRRICESVEIPVYA
                     IGGITVERCRDVLAAGAKGVAVASGMLPNIDRQTAAFLQALDAREP"
     gene            1950..2171
                     /locus_tag="AC16BC_04090"
     CDS             1950..2171
                     /locus_tag="AC16BC_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HIR3240829.1"
                     /translation="MGVRRLFLKLGDQVTHRRYPQWGLGEVVEERTAVTVGGMCFVRI
                     MFADGRERSFINNLDDVNCCYYAGIRVNA"
BASE COUNT          415 a          662 c          736 g          403 t
ORIGIN      
        1 ggttcctggc cgagaacgcc cagttcgccg aagcgtgcga ggcggcgggg ttcaagttca
       61 tcggcccgtc gcccgagagc atcgcgttga tgggcgacaa ggccaaggcg cgcgagttga
      121 tgatcaaata cggcgtgccc gtgattcccg gaagcacggg cgcgttgcga gacgaggcgg
      181 aggcgcgcga gacggccaag cgcatcgggt accccgtgat cgtgaaggcc tcggcgggcg
      241 gcggcgggcg gggcatgcgg gtggtcaacc aggaggacga cctcctcaag gcgattcaga
      301 ccgcgcagac cgaggccaag tccgcgttcg gggacgactc ggtgtatctg gagaagtact
      361 tcatcgaccc gcggcacatc gaggtccaga tcctggccga caagaagggc cacaccattg
      421 cgttgggcga gcgggactgc tccattcaac ggcggcatca gaaactgatc gaagagtcgc
      481 cctcaccggc cgtgagcccg gagttgcgcc gagaaatgat gaaggcggcg gtcgacgcgg
      541 tcaaagccgc caagtacgtc aacaccggga ccgtcgagtt cctgctggac aaacaccagc
      601 ggttctactt cattgaaatg aacacccgga ttcaggtcga acatcccgtg accgagatgg
      661 tcgtggacat ggacctgatc aaggagcaga tcatggtcgc ggcgggccgc tcgctcgcga
      721 tgaaacagcg ggatgtcaag ctgcgcgggc acgccatcga gtgccggatc aacgcggagg
      781 accccgagcg cttcacccca tcgcccggca cgatctccgt gtaccacgtg cccggcggcc
      841 ccggcgtacg cgttgattcc gcggcgtacc ccgggtgcgt gatcccgccg cactacgatt
      901 ccctggtggc caaggtgatc gtgacaggag agacgcgtct ggaagccatc gcgaggatga
      961 aacgcgcgct cgcggagttt cagatcgaag gcatcaagac cacgatcccg ctgcaacggc
     1021 ggatcatgga cgatcccaat ttcgtcaaag gcaagttctc cacgggattt ctggaacggt
     1081 ttctgtcggc cgacgaaccg gccgcgtaag cgcctgtcca tgagcggcca tctgcggcgt
     1141 tgtcgctgcg cgtccgctcc tcacgtacga cccggtacgc tgcggtgcgg gcgctcgctc
     1201 cgcccggcat ctgaccgctc ctgaacaggc ttattggctc cagttatgtc gacgcgaacg
     1261 agtcttccca tgttctcatc gccgctctat tggattgccg acgcctcgtt ggtcggcgag
     1321 cggtcgctcg tcgagttggc ggaagcggcc atcgggtccg gtctacgcgc gtttcagtat
     1381 cgcgacaaga cgcgttcgcc gcgggatcaa taccccattg ccctggcatt ggccgacctc
     1441 gccgggagaa cgggggcaac cttcgtcatc aacgacgaca tcgacctggc gttggtggtc
     1501 gaggcccatg gtgttcatct cgggcaggag gacttcccgg tcgccgaggc ccgccggatc
     1561 ctcggccgga agatgctcat cggctgttcc gtgcatacga tcgatcaagc caaagcagcc
     1621 gaggcggacg gagccgatta tctcggcgtg ggacccgtct acgagtccag cacaaaaatg
     1681 gctcgtcccc cgctggggtg cgagcagctt cgtcgcatct gcgagtctgt ggagatcccc
     1741 gtctacgcga ttggaggcat cacagtggag cggtgtcgcg atgtgctggc ggcgggagcg
     1801 aagggtgtgg cggtcgcgtc agggatgctt ccaaatatcg accggcaaac tgcggcattc
     1861 ctccaggcgt tggatgcccg cgaaccttga caacccgtcg cctgcgcgcc tagcatgaaa
     1921 ttgttgggcg gtgattcgtg gttcaggcga tgggagttcg gaggttattt ctcaaactcg
     1981 gcgatcaagt cacgcaccgg cggtatcctc aatggggcct tggtgaggtg gtggaggagc
     2041 gcacggcggt gacggtcggg gggatgtgtt tcgtgcgcat catgttcgcc gacggccggg
     2101 agcgttcgtt tatcaataac ctcgacgatg tcaattgctg ttactacgcg gggattcgag
     2161 tcaacgcgta atctaatcca gcaggtcctg caacaagccc gctcgttcaa gtcggt
//
LOCUS       DBNGTT010000179         2130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_802939, whole genome shotgun sequence.
ACCESSION   DBNGTT010000179 DBNGTT010000000
VERSION     DBNGTT010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_802939"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /gene="ybgF"
                     /locus_tag="AC16BC_04095"
     CDS             <1..745
                     /gene="ybgF"
                     /locus_tag="AC16BC_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR3240830.1"
                     /translation="VDRIAQQMGAFDKLLQDRSSSGQRSQVDLVVRLDQIGADVQTVQ
                     GRIEEQAHRISDLSAALDEQAFRLNQLSSKLDTADARLTEQERRTASGVAPAPSGTAP
                     PSESASDQERVVLPGRTPISGLSPVEAYNLAYNDFIKGSYDLAIQGFQAFLKQFPTSI
                     LVPHAVYWLGESYYQTKQYPQAIEQYERLTTQFPKNEKLVGALLKEGFAYAAVGDKTK
                     ARAALKRVLEEFPRASEANLAKEKLAELR"
     gene            991..>2130
                     /gene="mutL"
                     /locus_tag="AC16BC_04100"
     CDS             991..>2130
                     /gene="mutL"
                     /locus_tag="AC16BC_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3240831.1"
                     /translation="MPNALVDRIAAGEVIERPASVVKELIENALDAGARRIDVETACP
                     GGLWSITVSDDGEGMAQDDLTLAFERHATSKIGSLEDLDALSTLGFRGEALPSIASVA
                     RVRASSARRGAMDGAEIELDGGRVVRRAPSPPRVGTTMAVTDLFVHTPARRKFLKSAS
                     TELSHVCAAVQQHALARPEVAFSVTHEGRRVAEYPAARDLAERLIQVYGREAARDRLI
                     PVRRAQGPVEVLGYCSRPEIDRATRKGQDWYVNGRPVRHPVFLRAVDQAYATRLMTGR
                     HPVVALFLQMPGSEVDVNVHPAKREVRFAHPDEVARLVTKAIEEALARLDAQPPHATL
                     PGERSTETLSGDGMAAEPLAEYRSLVVDVSDAATPALPALGPLFRS"
BASE COUNT          358 a          680 c          737 g          355 t
ORIGIN      
        1 ggtggaccgc atcgcgcagc aaatgggcgc cttcgacaag ctgctgcagg atcgctcctc
       61 cagcgggcag cggagccagg tggatctggt ggtccggctc gaccagatcg gggccgacgt
      121 gcagaccgtc cagggtcgga tcgaggaaca ggcgcaccgg atctccgacc tgtccgccgc
      181 gctcgacgag caagcctttc gtctcaatca actcagttca aagttggaca ccgcggacgc
      241 ccggctgacc gagcaggagc gccggaccgc ctccggggtc gctccggccc cctccggaac
      301 cgcacccccg tccgaatccg catcggatca ggagcgcgtg gtgctcccgg gccgcacccc
      361 catcagcggg ttgagtccgg ttgaggccta caatttggcc tacaacgatt tcatcaaggg
      421 aagctacgat ctggcgatcc agggattcca ggcgttcctc aagcagttcc cgacgtcgat
      481 cctggttcca cacgcggtgt actggctggg agagtcgtat tatcagacca agcagtatcc
      541 ccaggccatc gagcaatacg agcggctgac cacgcagttc ccgaagaacg agaaattggt
      601 cggggccttg ttgaaggaag ggtttgccta cgccgcggtg ggggacaaga ccaaggcgcg
      661 cgccgcgctg aagcgggtgt tggaggagtt tccgcgggcg agtgaggcga atctcgccaa
      721 ggaaaagctc gccgaactcc gttagtcgga gcctgtccat gaacggccgt ctactgcgtt
      781 gttgctgcgc ttccggtgct cacgtacaca tggagtacgc tccgctccgg ttctcgcaac
      841 gccttgtatc cggccgattc ctggacaggc tcgcctcgaa gaagcccgag gcggtctcct
      901 aaaccaagga gacttgctcc cgttgtcatg accgcattcc caccagaagt gtttcccgcc
      961 acaagttcag ccgccgggcg aatccggcga ttgcctaacg cgttggtgga ccggatcgcg
     1021 gcgggggagg tgattgagcg accggcgtcg gtggtgaagg aactcatcga gaacgcgctg
     1081 gacgccgggg cgcggcggat cgatgtggag accgcgtgcc cagggggctt gtggtcgatt
     1141 acggtatccg acgacgggga ggggatggcg caggatgatc tgacgctcgc gtttgagcgt
     1201 cacgccacga gcaagatcgg atccctggag gatctcgacg cgctgagcac gttggggttt
     1261 cgcggagagg cgctccccag catcgcgtcg gtggctcgag tccgcgcgag cagcgcgcgt
     1321 cgaggcgcga tggacggcgc ggagatcgaa ctggacggcg ggcgagtcgt gcgtcgcgcg
     1381 cccagtccgc cccgggtggg aaccacgatg gcggtcaccg acctgttcgt gcacacgccc
     1441 gcgcggcgaa agtttctcaa gtcggcctcc accgaactct cgcacgtgtg tgcggcggtt
     1501 caacagcatg cgctcgccag gccggaggtc gcgttcagcg tgacccacga ggggcggcgc
     1561 gtggcggagt atcccgcggc gcgggatttg gccgagcgcc tcatccaggt gtacggacgc
     1621 gaggccgcgc gcgatcggct catcccggtc cggcgcgccc agggccccgt ggaggtgttg
     1681 ggatactgtt cccgtccgga gatcgaccgc gcgacgcgta agggacaaga ctggtacgtc
     1741 aacggccggc cggtccgtca ccccgtgttc ctgcgggccg tggatcaggc ctatgccacg
     1801 cgtttgatga ccgggcgcca tcccgtggtg gcgttgttcc tccagatgcc gggatcagag
     1861 gtggacgtca acgtgcatcc ggccaaacga gaggtccgct tcgcccatcc cgacgaggtg
     1921 gccaggctgg tcaccaaggc catcgaggag gcgctggccc ggctcgatgc gcagccgccc
     1981 cacgccacgt tgcccggtga acgctcgacc gaaaccttgt cgggcgacgg catggcggcg
     2041 gagcccttgg cggagtaccg ctcgcttgtg gtcgacgtgt ccgacgccgc cacgccggcg
     2101 ctcccggccc tggggccgct ttttcggtcg
//
LOCUS       DBNGTT010000180         5184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1697074, whole genome shotgun sequence.
ACCESSION   DBNGTT010000180 DBNGTT010000000
VERSION     DBNGTT010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1697074"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..522)
                     /locus_tag="AC16BC_04105"
     CDS             complement(58..522)
                     /locus_tag="AC16BC_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3240832.1"
                     /translation="MLKLSKKIDYGLLAMNYIALSHDSSSVNTKRIADEYHIPVELLA
                     KVLQRLAKTGLIVSQNGPKGGYALARPATEIRVSEIIEAIEGPIMLTTCSLVGKGDGA
                     CDQWSWCSLRSPLHKIQDGIRKLLQGMTLAEMSQISRQEEHSYGLEIAHLPR"
     gene            complement(682..1206)
                     /gene="purE"
                     /locus_tag="AC16BC_04110"
     CDS             complement(682..1206)
                     /gene="purE"
                     /locus_tag="AC16BC_04110"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR3240833.1"
                     /translation="MTTSSRPLVGIVMGSESDLPIMEEAGKALKELGVPYTMTIASAH
                     RAPDLLRSWIRSMEAQGVRVIIAGAGGAAHLAGVTASETLLPVIGVPLEASSLQGLDA
                     LLSTVQMPGGIPVATVAIGKAGAKNAGILAARILALQDDGLAKRLGEYRAKMAQDVEK
                     KAKSLAERLMSNPA"
     gene            1315..2520
                     /gene="pgsB"
                     /locus_tag="AC16BC_04115"
     CDS             1315..2520
                     /gene="pgsB"
                     /locus_tag="AC16BC_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HIR3240834.1"
                     /translation="MVTILLLLAGLTAYGIWEFQTHHRRLRRIPIRIHVNGTRGKSSV
                     TRLIAAGLRTGPRRTVAKCTGTTPRFLLEDGSELPIHRAGSPNIIEQLMIVSVAEARG
                     AEILVIECMAVQPHLQWISEERMIRSTLGVITNARADHLDQMGPTVFNVAEALANTIP
                     KHSTVYTAERAFRSVFERRAAANGSTLVAADADAVSDEEIAGFSYVEHKENVALALAV
                     CAHFGIERATALEAMQRATPDQGALQGYPIHFFAKNLLFYNAFAANDRDSTLMIAKRL
                     GLKNRPEQPVFWIVNNRGDRLQRAEQFGEMIAADIEAEHVFLVGDFTKATHDIAVRRG
                     FPPHRLSDLGHVGADALFEAVVARVERSATILGVGNIGGAGREIVTFFKNRSMEWSNR
                     PSVSGSFSA"
     gene            2478..2942
                     /gene="pgsC"
                     /locus_tag="AC16BC_04120"
     CDS             2478..2942
                     /gene="pgsC"
                     /locus_tag="AC16BC_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HIR3240835.1"
                     /translation="MVEQAIGLGLVLSLIFSESLGLAAGGMVVPGYIALYIHQPFLVV
                     GTVVASLLTLGTVKVLTNYILIYGRRRIVFSIILGFLFGWLAKNVFIISFPDYTVELK
                     SIGYIIPGLIANWMERQGAVKTLCVMVTTAVIVRLILMLISGEVIPIEVAVR"
     gene            2923..4083
                     /gene="pgsW"
                     /locus_tag="AC16BC_04125"
     CDS             2923..4083
                     /gene="pgsW"
                     /locus_tag="AC16BC_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HIR3240836.1"
                     /translation="MKWRSDKASLQALLFITVVSVLALALAEHTVTPVRESHYTQKMQ
                     AAKQALLAQHALRTIRGAQNVVVDPVNDPNGTGMIGSEFTLITTDRGDIGSKLTGTNP
                     NFAAVMVQMLTDAKLKKGDVVAVAMTGSFPALNIAALSAIEALGLRPIVVTSVGSSSW
                     GATDPEFTWLDMERALNEAGVLHTRSVAASIGGGKDVGRGLSPEGRRAIESAIARNKV
                     AFIHEPTVEASIDARLAAYDRHAAGKRIKAFVNIGGGIASLGNAISGTLVPAGLSTGL
                     SGKNFPMKGVLIRMAERDIPVIHILDVQRLAKRYGLPLDPVPLPELGQGPIYFKERYD
                     RVQLGLIIFVLIVLTTAVVRLDMRHYLQQLRAGQYGAGGSTVPPAPPPPKTP"
     gene            4104..4637
                     /locus_tag="AC16BC_04130"
     CDS             4104..4637
                     /locus_tag="AC16BC_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240837.1"
                     /translation="MFSIGTRFVAALIAAGAIAAVLTGTGHAAPRGKSRLISFTPDSY
                     VRTEIVATKSKERKYYVMTADKPIEFSVSGPTTAEISTRLLFSVDMKGEQRYTVSVTQ
                     DTLLDHAQEVAAMTFTTRKSPVSTLAGAGKLVPGEGRRLKLSVPSGRHQYRIHLSGTT
                     GREAGVRILIPRKDIRP"
     gene            4662..>5184
                     /locus_tag="AC16BC_04135"
     CDS             4662..>5184
                     /locus_tag="AC16BC_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240838.1"
                     /translation="MRTRSVPGSIVVGICLLATAHAKAAAPDLRAAVGVKIRFDDNVF
                     QYSDANLGRYDRTLAKFATIESTDDIIVTPSFDVRAVWKGRYATRVFAGVDLNRYTNN
                     TARNSETYTVGLAQNLSKADTLTLRYRFVPTRFGWRLADPPNQTVTYSDADLSTSSWR
                     LSLDHDFTRDLGGE"
BASE COUNT          997 a         1692 c         1534 g          961 t
ORIGIN      
        1 gtgaagtacg gcagcatggc ctggagcacg cgcggatcac acggcgtggt ggagtgatta
       61 tcgaggtaaa tgggcaattt caaggccata cgaatgctcc tcctgccggc tgatctgcga
      121 catctcggcc agcgtcattc cttgaagtag tttgcgaatc ccgtcctgaa tcttatggag
      181 cggcgaccga aggctgcacc acgaccactg atcgcaggcc ccatcgccct tgccgaccag
      241 cgagcaggtc gtgagcatga tcggcccctc gatcgcctcg atgatctccg acaccctgat
      301 ctcggtcgcg ggtctggcca gcgcgtaccc gcccttcggg ccgttctgcg acacgatcag
      361 ccctgtcttg gcgagacgct ggagcacctt ggcgagcagc tcaaccggaa tgtgatactc
      421 atccgcgatc cgcttggtgt tgaccgacga gctgtcgtgg ctcagggcga tatagttcat
      481 cgccaacagc ccgtagtcga tctttttcga gagctttaac acggcgagcc ctccatcatt
      541 atatatgcga ctgctttcat ccccgatcgt ttatatctgc gactaattaa gtcggataca
      601 aatataacga gttgaatgcc ttgaagtcaa gaacaatttg tttttggtga agattcggct
      661 gagcagcggc ctgtagggag gctaggccgg attcgacatc aggcgttcag ctaaagattt
      721 cgcttttttc tcaacgtctt gtgccatctt cgcccggtac tctccgagcc gcttggccag
      781 accgtcgtcc tgaagcgcca gaatacgcgc ggccagaatg cccgcatttt tagccccggc
      841 cttcccgatc gcgaccgtgg ccaccgggat acctcccggc atctggaccg tggacaacaa
      901 cgcgtccaac ccctggagcg aggacgcctc caacggcacc ccgatcacgg gcagtaaggt
      961 ctcggacgcc gtgacgccgg ccaagtgcgc ggccccgccc gcgcccgcaa taatcacccg
     1021 cacgccttga gcttccatac tacgaatcca cgaacgcaga agatccggcg cccgatgcgc
     1081 ggatgcgatg gtcatcgtat acggcacgcc gagttctttg agcgctttgc cggcttcctc
     1141 catgatcgga agatccgact cgctccccat cacgatgccg accaacggcc ttgacgacgt
     1201 ggtcatctga gaccctctct caaaaacatg ttccgatcag cgcgtgaccg tagcacggca
     1261 cgctgacacg ggtcaagctt gagcaagccc cgcccctatg gtagggtaac cgccgtggtc
     1321 acgatcctcc tgctgttggc gggacttacg gcatacggta tctgggaatt ccaaacgcac
     1381 caccgccgtc tccgccgcat tccgattcgc atccacgtca acggcactcg cggcaagtcg
     1441 agcgtcacgc ggttgattgc cgccggtctg cgcaccggcc ccagacggac cgtcgcaaaa
     1501 tgtaccggaa ccacgccccg ttttctcctt gaggatggga gcgagctgcc gattcatcgc
     1561 gccggttcgc ccaacatcat cgagcaactg atgatcgtca gcgtggccga ggccaggggc
     1621 gcggagattc tcgtgatcga gtgcatggcg gtccagccgc acctgcagtg gatctccgag
     1681 gaacggatga ttcgctcgac gctcggcgtc atcaccaacg cgcgcgccga tcacctggac
     1741 cagatggggc cgactgtttt caacgtcgct gaagcgctcg cgaacacgat cccgaaacac
     1801 tccacggtgt atacggccga gcgggcgttc cgctccgtct tcgagcgccg agccgcggcg
     1861 aacggctcga ccctggtcgc ggcggatgcg gacgccgtga gcgacgagga gatcgcgggg
     1921 ttctcctacg tggaacataa ggaaaacgtc gcattggcgc tggccgtgtg cgcccacttc
     1981 gggatcgagc gagccaccgc gctcgaggcc atgcagcgcg ccacgccgga ccagggggcg
     2041 ctccagggat atccgattca ctttttcgcg aagaacctgc tcttttacaa tgcgttcgcg
     2101 gccaatgacc gggattcgac gctgatgatc gcgaaacgac tgggtctgaa aaatcgcccc
     2161 gagcagccgg tgttttggat cgtgaacaac cgtggcgacc ggctgcagcg ggccgagcag
     2221 ttcggagaaa tgatcgccgc cgacatcgaa gcggagcacg tgttcctggt cggcgacttt
     2281 accaaggcca ctcacgacat cgcggtgcgt cgcgggttcc caccccaccg gctcagcgac
     2341 ctcggccacg tcggggcgga tgcgctgttc gaggcggtcg tggcgcgggt cgaacggtcc
     2401 gccacaatcc tgggggtcgg caatatcggc ggcgcgggga gagagatcgt cacgttcttt
     2461 aagaatcgga gcatggaatg gtcgaacagg ccatcggtct cgggctcgtt ctcagcctga
     2521 ttttctcgga atcgctgggg ctcgccgcgg gcggcatggt cgttcccggc tacatcgcgc
     2581 tgtacatcca ccagccgttt ctggtggtcg gaaccgtggt ggcgagtctg ctcacgctgg
     2641 gaaccgtgaa agtcctgacc aactacattc tgatttacgg gcgccggagg atcgtctttt
     2701 ccatcatcct cggttttctc ttcggctggc tcgcaaaaaa cgtcttcatc atctcgttcc
     2761 cggactatac cgtagaactc aaatcgatcg ggtacatcat cccgggcctg atcgcgaact
     2821 ggatggagcg tcaaggcgcg gtcaagacct tatgcgtcat ggtgaccacc gcggtcatcg
     2881 tacgattgat tctcatgctc atttccgggg aggtcattcc gattgaagtg gcggtccgat
     2941 aaagcctcac tgcaagccct gctgttcatc acggtcgtct ccgtgttggc gctcgctctc
     3001 gcggaacaca ccgtcacccc ggtgcgcgaa tcccactaca cgcagaagat gcaggcggca
     3061 aaacaggccc tcctcgcgca acacgcgttg cgcacgattc gaggcgccca aaacgtcgtg
     3121 gtcgatccgg tcaacgaccc caacggcacc gggatgatcg gcagcgaatt cacgttgatc
     3181 accaccgacc gcggggacat cggctccaag ctgaccggca cgaacccgaa cttcgccgcc
     3241 gtgatggtcc agatgctgac cgacgccaaa ctcaagaaag gcgacgtggt ggcggtggcc
     3301 atgaccggat cgttccccgc cctcaacatc gccgcgctct cggcgatcga ggcgttgggc
     3361 ttgcgaccca tcgtcgtcac atcggtcggg tcctcctcgt ggggagccac cgaccccgag
     3421 ttcacgtggc tcgacatgga gcgcgcgctg aacgaggccg gcgtgctcca cacgcgctcg
     3481 gtcgcggcct cgatcggggg cgggaaggac gtgggacgcg gactctctcc cgagggccga
     3541 cgggcgatcg aatccgcgat cgcccgcaac aaggttgcct tcatccatga gccgaccgtg
     3601 gaagcgtcga tcgacgcgcg gttggcggcc tacgaccgcc acgcggccgg caaacggatc
     3661 aaggcgttcg tgaatatcgg aggcggcatc gccagtctcg gcaacgcaat cagcggtacg
     3721 ttggtcccgg ccgggctgtc caccggcctc agcggcaaga acttcccgat gaagggtgta
     3781 ctgatccgca tggccgaacg ggatatcccg gtcatccaca tcctggatgt gcagcgcctc
     3841 gccaagcgct acgggctccc gctcgacccg gtcccgctgc cggaacttgg acagggcccg
     3901 atctacttta aggagcgata cgatcgtgtc caactcggct tgattatctt cgtcctcatc
     3961 gtgttgacta cggccgttgt ccggctcgat atgcgacatt acttacaaca gctccgggcc
     4021 gggcagtacg gggccggcgg ctcgaccgta ccccccgcgc cgcctccgcc gaaaacgccg
     4081 tgaccccaga aaggaaatcg gacatgttct ctattgggac gcgcttcgtc gcggcgttga
     4141 tcgccgccgg cgccatcgcg gcggtactga ccggaaccgg gcacgcggcg ccacggggca
     4201 aatctcggct gatctcgttt acccccgaca gctacgtgcg gaccgaaatc gtggccacca
     4261 agtccaaaga gcggaaatac tacgtgatga ccgcggacaa gcccatcgaa ttttccgtga
     4321 gcggccccac gaccgcggag atttcaaccc ggctgttgtt ctccgtggac atgaagggcg
     4381 agcaacgcta caccgtcagc gtcacgcaag acaccctgtt ggaccacgcg caggaagtcg
     4441 ccgccatgac cttcacgacg cgcaagtcgc cagtttcaac cctggccggc gccgggaaac
     4501 ttgttccggg cgagggacgg cgtctgaagc tcagcgtgcc gtcaggacgc caccagtacc
     4561 ggatccacct gtccggcacg accgggcggg aagccggggt cagaatcttg atccccagaa
     4621 aagacattcg tccgtaacct cgtcattatc gggagaatgg tatgcgcaca cggtctgtcc
     4681 cgggaagcat cgtcgtcggt atctgcctgc tcgccactgc acacgcgaag gccgccgcgc
     4741 ccgatctccg cgccgcggtc ggcgtgaaaa tccggttcga cgacaacgtc ttccagtatt
     4801 cggacgccaa ccttggccgc tacgatcgga cactcgcgaa attcgcgacc atcgaatcga
     4861 ccgacgacat catcgtgacc ccgtcgttcg acgttcgcgc cgtgtggaag ggccggtacg
     4921 cgacccgcgt gttcgcgggc gtggacctga accggtatac gaacaacacc gcgcgaaaca
     4981 gcgagacgta caccgtcggc cttgcccaaa acctctccaa ggccgacacg ctcacgctgc
     5041 gctatcggtt cgtgccgacc cgcttcggtt ggcgcctggc cgatccgccg aaccagaccg
     5101 tgacgtactc cgacgccgac ttgtccacgt cctcatggcg cctcagcctc gatcatgact
     5161 tcacccggga cctcggcggc gaag
//
LOCUS       DBNGTT010000181         1577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2975328, whole genome shotgun sequence.
ACCESSION   DBNGTT010000181 DBNGTT010000000
VERSION     DBNGTT010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2975328"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1492)
                     /locus_tag="AC16BC_04140"
     CDS             complement(218..1492)
                     /locus_tag="AC16BC_04140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR3240839.1"
                     /translation="MTLRHILIVASGAWIVIAVGSLIGMAALFVGSASVTVPTAVVGL
                     VAGVGGFAALTAVAAAVASRRGRSGWTARSLSDRGPDLQQAVHELERSHKEMAAVSRY
                     AESCTKLQEPGELYRTLTRQMAQAIDASLCFVMRYEKAANKMVAQIPGYGVADEILRA
                     TRYTVTPEIKAVWNFSTQGSLLSNDPQGDRRVLREISRNLGLFNCVVVPFFFQERVAG
                     LIVAGNKSKWFTYDDIRLLTAFSSYTSLAVANQQLQQEVRRTIRDGLTGLYTAQYLRS
                     LLDQAAAAPTPLTAGPSLIAIAIDDFQSCLDLYGRSGADRIIKDVAGLLLGYVGPTGH
                     VARYGTHEFIVLLPGSDREDPASAAERIRGLIEEHPVAVDTGIPFAVTVSIGVASSTG
                     AVSSEALLHAAQRALHRARQRGPNQIAAACAE"
BASE COUNT          308 a          520 c          480 g          269 t
ORIGIN      
        1 tccgatccgc cgcactttcg gcatagccgg tcctcctgat tcgccacatt gtacacccct
       61 tgctggatga aaagacgcgg ccagcctact cgccggcggt tcgggaacgg agacggacgg
      121 tcagccgtgc aacgcgatga tcaggccgga cgcgcctgag gcgggtatcg ccgcccgcct
      181 gagcggggtc gagcgctcgc tcgacgaccg cctttcacta ttccgcgcag gccgcggcga
      241 tctggttggg gccgcgctgc ctggctcgat gcagggcgcg ctgggccgcg tggagcaagg
      301 cctcggacga aaccgccccc gtggaggagg caaccccgat acttacggtc acggcaaacg
      361 ggatgcctgt atcgaccgcg accgggtgct cctcgatcaa ccctcggatg cgttcagccg
      421 cggacgcggg gtcctcgcga tcgctcccag gaagcagcac gatgaactcg tgggtgccgt
      481 accgcgccac gtgtccggtg ggaccgacgt acccgagcag cagccccgcg acgtctttga
      541 taattcggtc cgcgcccgac cgtccataca ggtccagaca ggactggaag tcgtcaatcg
      601 cgatggcgat cagggatggc ccggcggtca aaggcgtggg agcggcggcc gcttgatcga
      661 gaaggctacg aagatattgc gccgtgtaga gccccgtgag accgtctcgg atcgtcctgc
      721 ggacttcttg ttgaagctgc tggtttgcca ccgccaacga ggtataactc gaaaacgcgg
      781 tcagcagcct gatatcgtca tacgtaaacc acttggattt gttccccgcc acgatcaacc
      841 cggccacccg ctcctggaaa aagaacggca ccaccacaca attgaacaac ccgaggttcc
      901 gggagatctc tcgcagcact cgccggtctc cctggggatc gttgctcaga aggcttcctt
      961 gggtcgaaaa gttccagacg gctttgatct ccggggtcac cgtgtaccga gtggccctga
     1021 ggatttcatc cgcgacgccg tatcccggaa tttgagccac catcttattg gccgcttttt
     1081 cgtatcgcat gacgaaacag agggaggcgt cgatggcctg agccatctgg cgggtcaagg
     1141 tccgatataa ctcgccgggc tcctgaagct tggtgcacga ctccgcgtag cgggagaccg
     1201 ccgccatctc cttgtgggat cgctccaatt catggacggc ctgctgcaaa tccgggccgc
     1261 gatccgagag ggatcgcgcc gtccaacccg atcgtccacg gcgggacgcg accgccgcgg
     1321 ccaccgccgt cagggcggcg aatccaccca cgcccgccac caagccgacc accgcggtgg
     1381 ggacggtcac ggacgccgac cccacgaaca gcgcggccat gccgatcaac gaccccacgg
     1441 cgatcacgat ccacgcgccc gatgccacga tgagaatatg acggagcgtc attgactttc
     1501 cctctcttca cagatatgaa ccgaccggga aatcacctga accatggcga tctgaaagat
     1561 aattccggag tcatgcg
//
LOCUS       DBNGTT010000182         2086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_676080, whole genome shotgun sequence.
ACCESSION   DBNGTT010000182 DBNGTT010000000
VERSION     DBNGTT010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_676080"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="AC16BC_04145"
     CDS             complement(<1..289)
                     /locus_tag="AC16BC_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240840.1"
                     /translation="MRLVMPREGERALSVLQALSAEVARAPEVAEALNGVLDGLLRLT
                     RADTGAIHLISGDGSDLHLVASRGLSVRFQRMECRIPHGVCLCGRAVDGDEP"
     gene            complement(605..1555)
                     /locus_tag="AC16BC_04150"
     CDS             complement(605..1555)
                     /locus_tag="AC16BC_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3240841.1"
                     /translation="MIAHPLNVLVVHGIGEQPDTFADEFERVLSGHVRRLIGELTARY
                     RLSDPPSPRVLNVIPGLWAPVPEGIFKVLESRLFAGRADRMRALAMRFMGDVIAYQGP
                     AVYEEIHRALAHALRSRLSPESRHLTIVAHSLGTVIASDFVHDHTRRAGTRFRDHFGV
                     DFVNFFTLGSPLALYAARSPLSVMDGDGSVRVLDGFDAPVRVESERGVWVNVYSDDDV
                     VGYPLKHVNDAYARAVTADVVHDAGNLLTRWNLFSHGAYWTEPDVITMIAEKLAIDYA
                     EAHLGLRSPALDEALAGYRGRLAPVKRDERAGSKVQGPRT"
     gene            complement(1562..>2086)
                     /locus_tag="AC16BC_04155"
     CDS             complement(1562..>2086)
                     /locus_tag="AC16BC_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3240842.1"
                     /translation="PPAYGVELTGTYPGIAPDVTKKLAAMMHDWVEQVMVRGARVEWH
                     LEMGSAFAEILAAVERYGADLIVMGTHGRTGLSHLLLGSVAARVVQHAPCPVLTVKAG
                     AAAPAPATAAPAAPAPRRERPSKCMICGAASEETICATCKARVQAEALARKQGAEKEG
                     RSDDWKFAGPGEWR"
BASE COUNT          360 a          763 c          629 g          334 t
ORIGIN      
        1 cgggttcgtc cccgtccacg gcgcgcccgc acaggcagac gccgtgggga atccggcact
       61 ccatccgctg aaaccgcacc gacaacccgc ggctcgccac caggtgcagg tcgctcccgt
      121 ctcccgagat cagatggatg gcgccggtgt cggcccgggt cagacgcaac aacccgtcga
      181 gcaccccgtt taacgcctcc gccacttccg gggcgcgggc cacctccgcg ctcagcgcct
      241 gcaacacgct taatgcgcgc tctccctccc gaggcatcac caaccgcacg aactctccgt
      301 cctcaccgtc gtgaacctga acccgatccc gttccgacat ggcagaagaa agcgccgctc
      361 ccatacgcac acctcctgta caacgagctg cccgttccgg tgatcgatca ccattgcctt
      421 gaatgactcc ttcgagtccg gttcgaggaa ccggccctgc ggggaatgcc agcgtcgtta
      481 ttttggacta gatcacctgg aattgaaaca ccccaaatgg agtgttttgg gaagaaagat
      541 cccgtatttt gaggagggtt tcgcccccct cagggacatc gggtcacgag gcccctcgat
      601 cggactaagt cctgggccct tggacctttg aaccggcccg ttcatcacgc ttcaccggcg
      661 ccaaccggcc ccgatacccg gccagggcct cgtccaacgc cgggctccgg aggcccaaat
      721 gcgcttcggc gtagtcgatg gccagctttt ccgcgatcat ggtgatcacg tccggttcgg
      781 tccaatacgc gccgtggctg aacagattcc atcgcgtcaa caggttaccc gcgtcatgga
      841 ccacgtcggc ggtcacggcg cgcgcgtagg cgtcgttgac gtgtttgagc ggatacccca
      901 ccacgtcgtc gtcgctgtag acattgaccc acaccccgcg ctccgactcc acgcgcaccg
      961 gcgcgtcgaa gccgtcgagc acgcggaccg acccatcccc gtccatcacg gacaggggcg
     1021 accgcgcggc gtacaacgcg agcggcgagc ccagcgtgaa gaaattcacg aagtccacgc
     1081 cgaagtgatc gcggaatcgc gtgcccgcac gccgcgtgtg gtcatgcacg aaatccgacg
     1141 cgatcaccgt ccccagactg tgcgccacga tggtgaggtg acggctctcc ggactgagcc
     1201 ggctccgcag cgcgtgggcg agcgcccggt ggatttcctc gtacacggcc ggtccctggt
     1261 acgcgatcac gtcccccatg aaccgcatgg cgagcgcccg catccggtcg gcgcggcccg
     1321 cgaacagccg cgactccagg accttgaaga tgccttccgg aaccggcgcc cacaagccgg
     1381 gaatcacatt gagcacgcga ggagacggcg ggtccgacag acggtatcgc gcggtcagtt
     1441 cgccgatcag gcgccggacg tggccggaca ggacccgttc gaactcgtcg gcaaacgtgt
     1501 cgggctgttc gccgatgccg tgcacgacca agacgttgag gggatgggcg atcatcggcg
     1561 atcatcgcca ctcgccgggt cccgcgaact tccagtcgtc gcttcggccc tctttttcag
     1621 cgccctgttt tctggcgagc gcctcggcct ggacccgcgc cttgcaggtc gcgcagatgg
     1681 tctcctccga cgcggcgccg cagatcatgc actttgacgg ccgctcccgc cgcggcgcgg
     1741 gcgctgcggg cgcggcagtc gccggagccg gcgcggccgc tcccgccttg accgtcaaca
     1801 ccggacacgg cgcgtgctgc accacccggg ccgccacgct ccccaacaac agatgcgaca
     1861 agccggtgcg gccgtgggtg cccatcacga tgagatccgc gccgtaacgc tcgaccgcgg
     1921 cgagaatttc cgcgaacgcc gagcccatct ccaggtgcca ctccacgcgc gcgccccgca
     1981 ccatgacctg ctcgacccag tcgtgcatca tggcggccag ttttttggtc acgtcgggcg
     2041 caatacccgg gtaggtcccg gtcaattcca ccccgtacgc gggcgg
//
LOCUS       DBNGTT010000183         2791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_547534, whole genome shotgun sequence.
ACCESSION   DBNGTT010000183 DBNGTT010000000
VERSION     DBNGTT010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_547534"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /locus_tag="AC16BC_04160"
     CDS             <1..2052
                     /locus_tag="AC16BC_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240843.1"
                     /translation="HQTGRKLLLYPPTHALIPKAIAGAIQRFAALWAHHPEVTVTVGA
                     KHLLLAGEPLDPQNPALAELTRALSVAHVARVTFRQHLDGDGLMRFFGWLHAKSADVD
                     ARQTNLAAFHQAVPAITIALVSFRDAVADTTEGARGGTWDQLLDALLEAGVGEQSQAE
                     GLAASERERPERVAEMVNRAAASGNGERYEQLILEHLQRLAAPLRDPVTGEARAAALP
                     EAERRRLVTLIERLDPPVRERLFRLAFNPESQGPGALVAVLDQLSEPVLLEVLCQIQA
                     HQGAISIPTLELLRKFVGVSREQPALAPSIREHLDGLDLTDRHALYAELFHKKTEKNF
                     YPESYAATIKGMSGAVPTGLTVPHEPVNDLVDPVGEAEIHRHLVTVMTELIQHPTAAA
                     NHSELIDRLGRALEDPMADGYADMAFHIFESLKHVRPDDPASREAFQATVLRLIAPVV
                     RALPKLDKTRGTAVADHLVAIGEPVVRTLLDILNSAPDMVLRKRLLDILVRIGRPIAP
                     AVMSWLGDQRWFIVRNMIYLLGETKVTEAAPKIIPFAGHSSEQVRIETLRALGQITPL
                     EQTLAIFSSATEDRDERVASCAIGILCQRPTPKVVDCLQRLFEAEDKRLSAPRKLKII
                     EALTRGKDPSCLALLSRLARRRRFILFDLPKQAAIRKAAREALRKHRLMVSTEEHDRA
                     A"
     gene            2036..>2791
                     /locus_tag="AC16BC_04165"
     CDS             2036..>2791
                     /locus_tag="AC16BC_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3240844.1"
                     /translation="MTAQPEWSLSPIAADPRYRLLKHLSRVLRGRSLYPPSHPQLREA
                     SKELVESLDSVFEGRDEVRIVALDDRLFVDDVRSLEVEMIAHDLRAKLKTQGIELLVL
                     AKGLEGHELLALVEGLASGNIPALPHVGTGKIGTDREPSQETKAAVPPVDMRPQVQAL
                     RQIFEGWDEIQGLVMDQVEHIMRVLEDQLFANQSSLISLASLKSYDEYTYTHAINIAI
                     LTMAQAESLGVPKHLIHDFGVAAMLHDIGKTLVP"
BASE COUNT          494 a          950 c          891 g          456 t
ORIGIN      
        1 catcagacgg gtcgcaaatt gctgttgtat ccgccgaccc acgccctcat tcccaaggcg
       61 atcgcgggcg cgattcagcg attcgccgcg ctgtgggcgc accatcccga ggtgacggtc
      121 acggtcggcg ccaagcacct cctgctggcg ggcgaaccgc tcgatccgca gaacccggcg
      181 ctcgccgagt tgacgcgcgc gttgtcggtc gcccacgtcg cccgcgtcac gtttcgccag
      241 catcttgacg gcgacggact catgcggttt ttcgggtggc tccacgccaa aagcgcagac
      301 gtggacgcgc gccaaacgaa cctggcggcg ttccatcagg ccgtccccgc gatcacgatc
      361 gcgctggtca gttttcgcga cgcggtcgcg gacaccactg agggcgcgcg cggcgggacc
      421 tgggatcaac tcctggacgc gctgctcgaa gcgggcgtcg gggagcagag ccaggccgaa
      481 gggctcgccg cgtctgagcg cgaacgcccc gaacgcgtcg cggaaatggt caaccgcgcc
      541 gcggcaagcg gcaacggaga acggtatgag cagctcatcc tcgaacacct gcaacggctc
      601 gccgcgccgc ttcgcgaccc ggtcacggga gaggcccggg ccgccgcgtt gcccgaagcc
      661 gagcgccgcc ggctcgtcac cctgatcgaa cgcctcgacc cgccggtgcg ggagcggttg
      721 ttccgcctcg cgttcaatcc cgaaagccag gggcccggcg cgctggtcgc ggtcctggat
      781 cagttgtccg aaccggtcct gctcgaagtg ctctgccaga ttcaggcgca ccagggcgcg
      841 atctccatcc cgacgctcga acttctgcgg aaattcgtcg gcgtcagtcg ggagcagccc
      901 gcgttggcgc cgtcgatcag ggagcatctc gacggtctgg acctcaccga ccgccacgct
      961 ttgtacgcgg agttgttcca caagaaaacc gagaagaact tctatcccga aagctacgcg
     1021 gccaccatca aagggatgtc gggcgccgtg cccacgggcc tcaccgtccc gcatgagccg
     1081 gtcaacgatt tggtcgatcc ggtcggcgag gcggaaattc accgtcacct ggtgacggtc
     1141 atgaccgagt tgattcaaca cccgaccgcg gcggccaacc attcggaact gatcgatcgg
     1201 ctggggcgcg ccctggaaga cccgatggcc gacggctacg ccgacatggc ctttcacatc
     1261 ttcgagtcgc tcaaacacgt gcgtcccgat gaccccgcgt cccgcgaggc gtttcaagcc
     1321 acggtcctcc ggctgatcgc gccggtggtg cgggcgctcc ccaagctcga taaaacccgc
     1381 ggcacggctg tcgccgacca cttggtggcc atcggcgagc cggtggtgcg gaccctgctg
     1441 gacattttga acagcgcgcc cgacatggtg ctgcgtaaac gcctgctcga catcctcgtg
     1501 cgcatcgggc ggcccatcgc gcccgcggtg atgagctggc tcggggatca gcggtggttc
     1561 atcgtgcgca acatgatcta cctcctgggt gaaacgaagg tcaccgaggc cgcgcccaag
     1621 atcattccct ttgcgggcca ttccagcgaa caggtccgca tcgagacgct gcgggccctc
     1681 gggcagatca cgccgttgga gcagaccctc gccattttct cgtccgcgac cgaagaccgc
     1741 gacgagcgcg tcgcgtcgtg cgcgatcggc attctgtgcc agcgccccac cccgaaggtc
     1801 gtggactgtt tgcagcgcct gtttgaggcc gaggacaaac gactgtccgc gccccgcaag
     1861 ctcaagatca tcgaagcctt gacccggggc aaagatccgt catgcctggc gttgttgagc
     1921 cggttggcca gacgccgccg cttcattctc ttcgacctgc ccaagcaggc cgcgatccgc
     1981 aaggccgccc gcgaggcgct ccgcaaacac cgattgatgg tttccacgga ggaacatgac
     2041 cgcgcagcct gagtggtcgc tctcgcccat cgcggcggac ccgcggtatc ggttgctcaa
     2101 acacctcagc cgggtgctgc gcgggcggtc attgtatccg ccgtctcacc cccagcttcg
     2161 cgaggcgtcg aaggaactgg tcgaaagcct cgacagcgtg ttcgagggac gggacgaggt
     2221 gcggatcgtg gcgctcgacg accggctgtt cgtggacgac gtgcggagcc tggaagtcga
     2281 aatgatcgcg cacgatctcc gggccaagct caagacccag ggcatcgagt tgctcgtgct
     2341 ggccaagggc ctcgaaggcc acgagttgct cgcgttggtc gaggggctgg cctccggcaa
     2401 catcccggcg ttgcctcacg tcgggaccgg caagatcggg accgatcgcg aaccgtccca
     2461 ggagaccaag gccgcggtcc cgcccgttga catgcggccg caggttcagg cgctgcggca
     2521 aatcttcgaa ggctgggacg aaatccaggg cctcgtgatg gatcaggtgg aacacatcat
     2581 gcgggtgctg gaggaccaac tgttcgcgaa ccagtcgtcc ttgatctcgc tggcaagcct
     2641 caagagctac gacgagtaca cgtacacgca cgccatcaac atcgccatcc tcaccatggc
     2701 gcaagcggaa tcgctcggcg tgcccaaaca cttgatccat gacttcggcg tggccgccat
     2761 gctgcacgac atcggcaaga ccctggtgcc g
//
LOCUS       DBNGTT010000184         8293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2209207, whole genome shotgun sequence.
ACCESSION   DBNGTT010000184 DBNGTT010000000
VERSION     DBNGTT010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2209207"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="AC16BC_04170"
     CDS             <1..899
                     /locus_tag="AC16BC_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240845.1"
                     /translation="IPAVVAGALVLVWTGWTIERNRAWKTEWSLWTDTAAKAPTNPRA
                     LATLALAHRDRGELDRAVELLRAATRANPEYHHAYGQLGLALIDQGNLDGALAAFERA
                     HALDPTDPQDTYHLGVIYQKLHRPGEAARAFNDTVRLRPDDAEAHNNLGALHQEAGQW
                     EDALVEYAAAVRLDPALPHAHYGMARVLEHQGRVEEAMREYQSTIQRNPRHVDAHVAL
                     AGLYVARGEKAAAVDTFEAALRLAPDVPEGHYQVGRLLDELDRRPEALAHYRRYLELA
                     PMSQANARAWVQARVRTLEAAR"
     gene            complement(915..1124)
                     /locus_tag="AC16BC_04175"
     CDS             complement(915..1124)
                     /locus_tag="AC16BC_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240846.1"
                     /translation="MYIIKLDGEVVTVTNSFDSAACYADGIRGVGRRVAITLAMPDDL
                     PPALRALEQRSGAARGRSPRMAAAV"
     gene            complement(1449..3287)
                     /locus_tag="AC16BC_04180"
     CDS             complement(1449..3287)
                     /locus_tag="AC16BC_04180"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR3240847.1"
                     /translation="MLRSNPEDRVAEDGMVRLEEPQLSANALETLKARYLLRAPDGAI
                     DETPAQLFGRVARAIADAELRFGSAAQAGAWEERFLAAMTRLEFLPNSPTLMNAGTPI
                     GQLSACFVLPIEDSIDGIFATLRQAALVHQTGGGTGFNFSAIRPAGDQVRGAVGVASG
                     ALSFMRVFNAATEAMRQGGKRRGANMGILNADHPEIEAFIAAKREPGALRNFNLSVGV
                     SDAFMEAVERGGDWVLRNPRNHAVTATRKAPELYQRLCEAAWATGDPGVVFLDAIERA
                     NPTPTAGSIRATNPCGEVPLLPYESCNLGSINLARCLRRVATGYAVDWDKLRELVRLG
                     VRFLDNVVEANRYPIPELERMALRHRKIGLGVMGLAETLIRLGLSYESQAARETGSEI
                     ARVLRETADAASAGLAAERGVFPGWSESVFAASGRRVRNATVLSIAPTGTISLIAGTT
                     SGIEPLFGVAYTRSHVLGGRALPEVSSLFAEAMDARGVPWQALVPVIHKTGSVQGVAG
                     VPEDVQRVFRCALDIAPLDHLRMQAAFQAHVDNAVSKTVNLRFAATVKDVDTVYREGH
                     RMGLKGITVFRYGSRSEQVLSLGAHPHDERFDLAHLASCDPGSCRV"
     gene            3442..3921
                     /locus_tag="AC16BC_04185"
     CDS             3442..3921
                     /locus_tag="AC16BC_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3240848.1"
                     /translation="MPTAKATPFAVKTILVPTDFSEGSQAALDYAVNLAKSFGSKIVL
                     LHVIETTTYTMTEALQLVNVYEVVRTAVEPVLDQMVTDLQQQKLSVSRAVAQGASAHE
                     IVSQAREVGADLIVMGTHGRRGVNHLMVGSVAERVVRTAACPVLTVRQPVTGAGQTK"
     gene            3969..4373
                     /locus_tag="AC16BC_04190"
     CDS             3969..4373
                     /locus_tag="AC16BC_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HIR3240849.1"
                     /translation="MNTIYPRSPKQLLGGYAHLARMIDKARAKAAGTLGEYIYPCPLD
                     QALLEFLGLDADAVFDVAKRGTDDEVLRWGQERATPRSAKDIAAWNQTFFARKPTDEK
                     KLQYFIEIRNRVAPHRTDVTTWVDLLDLEEGR"
     gene            complement(4471..5433)
                     /locus_tag="AC16BC_04195"
     CDS             complement(4471..5433)
                     /locus_tag="AC16BC_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3240850.1"
                     /translation="MSDCRTTGRRANALMGVTVAVGAALAFGWGTAEAKTVKVDFTAV
                     ETQVVVDNKGTTLNAWTFNNQFPGPVVRATEGDTIEFSLTNPKTNRAAHSMDFHAGEM
                     DFLTNHKPVQPGETHKITFTPTKPGIFFYHCGVGPMIQHIARGMMGAIIIDPKDAKAL
                     PKADREYVLVQSELWLNPDDVQAMFDRKYDHAVFNGGVFKYHPFLGGKPLEAKPGERV
                     RIYMVNAGPNNFSAFHPIGEIWENAWASGNPANRLTGVQTVVVGPGDAYTVDVIPQNE
                     GGYPLVTHSLTDALRGAIAVLVVKKDAQTLPLMPYVEQGKPSTP"
     gene            complement(5661..6335)
                     /locus_tag="AC16BC_04200"
     CDS             complement(5661..6335)
                     /locus_tag="AC16BC_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3240851.1"
                     /translation="MLDLPILSALTEAGRKDLSAHMAEVRYEKDEFIFKEGDPAEAFH
                     VLLSGTVKCVKSSPEGKEAVLKVLAPGDLFCCEAAVFDGGEHPGCAKPMGPVKVLKIG
                     KQAYFRLLKDNPEAALEIIRYLGHRLNESQETAKTFALERADQRVASLLARLAARVGK
                     DIPGGLLLDMQLSRQDIADMTGLALETTIRTISKLTRDGLLGKSGRSIVVKDLKALKA
                     LTASPA"
     gene            complement(6398..>8293)
                     /locus_tag="AC16BC_04205"
     CDS             complement(6398..>8293)
                     /locus_tag="AC16BC_04205"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR3240852.1"
                     /translation="YSCEDLFHQQALLDACREALAKVVEASRELPVISIVGLPLQVDA
                     LLFNCAAVVHRGKILGVVPKTYLPNYREFYEMRQFVSADAAHRDAIDLLDQRGVPFGN
                     RLLFHIDNQPLATFHVEICEDLWVPIPPSSYAALAGAAVLINLSASNSTVGKADYRRQ
                     LVAGQSSRNIAAYVYSAAGVGESTTDLAWDGHGMIYENGAPLAEATRFADGPQLITGD
                     VDLDRLAQDRMRQTSFGQAVSRHREAASTFRAISCSPDLPKSGRIPLARRYERFPYVP
                     GDPALRDERCREVYEIQVQGLVTRLRSIGPDAKIVIGVSGGLDSTQALLVCAQAMDRM
                     KRPRAHILGYTMPGFATSDRTLDQAKRLMKAVGCEAHEIDIKPSCLRMFQDLGHPFAQ
                     GKPVYDVTFENVQAGERTSHLFRLANQRGGIVVGTSDLSELALGWCTYGVGDHMAHYH
                     VNASVPKTLIQYLVRWVSRTHQLGENVSGVLDDVLATAISPELLPAGKGERGASQPAQ
                     RTEDVIGPYALQDFTLYYTLRFGYRPSKVAFLAWSAWHDAQEGLWPDIPEAERCAYSI
                     GDIKRWLRVFAQRFFHDSQFKRSCIANAPKVGSGGSLSPRSDYRAPSDADAAVWLRDV
                     ETIPEAE"
BASE COUNT         1469 a         2839 c         2594 g         1391 t
ORIGIN      
        1 ggatcccggc ggtggttgcg ggtgcgctcg tgctcgtctg gacgggatgg accatcgagc
       61 gcaaccgggc gtggaagacc gagtggtcgc tctggaccga caccgcggcc aaggcgccga
      121 ccaatccccg cgcgctcgcc actctggccc tggcgcaccg ggaccgcggc gagttggacc
      181 gcgcggtgga gttgctccgg gccgcgaccc gcgccaatcc cgagtatcac cacgcgtacg
      241 gacaactcgg tctggccctc attgatcaag ggaatcttga cggcgcgctc gccgcgttcg
      301 aacgcgccca cgcgctcgac ccgaccgatc cgcaggacac gtaccacctc ggcgtgatct
      361 atcagaaact gcatcggccg ggcgaggccg cgcgcgcgtt caacgacacg gtccggctca
      421 gaccggatga cgcggaggcg cacaacaatc tgggtgccct gcaccaagag gccggccaat
      481 gggaggacgc gctcgtggaa tacgccgcgg cggttcgcct cgatcccgcg ttgccgcacg
      541 cgcactatgg catggcacga gtcctcgaac accagggacg agtcgaggag gccatgcgcg
      601 aataccagtc aacgatccag cgcaatcccc ggcacgtgga cgcgcacgtg gcgctcgccg
      661 gattgtatgt ggcccgaggc gagaaggccg cggcagtgga cacgttcgaa gcggccctgc
      721 gcctggcgcc cgacgttccg gagggccact atcaagtcgg ccgcctgctg gatgagttgg
      781 atcgccgacc cgaagcgctc gcgcactatc gccggtactt ggagcttgcc ccaatgtccc
      841 aggccaacgc tcgcgcctgg gtgcaagcgc gggtccggac gctcgaggcc gcgcggtgag
      901 gccgacgcgg tggttcagac cgcggcggcc atccgggggg atcgtccccg cgcggcgcct
      961 gatcgttgct ccagggcgcg cagcgcgggg ggcaggtcgt cgggcatcgc gagcgtgatg
     1021 gccacccgcc gccccacccc gcgaatcccg tcggcgtaac acgcggccga gtcgaacgag
     1081 ttcgtgaccg tgaccacctc cccatccagc ttgatgatgt acatgtcgcc tccccgtcgg
     1141 ttcgaaggtg ggaaggaccc ccctcccctc gccccccaga gagagcaaat acagtgccat
     1201 ggcctgagcc ccaaaaggcg cgttccattc actcgcctca gctccgaccg acacaactgt
     1261 ccgaccagca cacacaagtg tcggacgaca gacatccata gtgtgacgca tgattcgacg
     1321 acagtcggag gctgctcaaa aagctccagc tgcaaggccg cagggaggaa gaaaccggag
     1381 cgtactgtgt cgtacgtgag gatttctgac gaccgagaac gccgcagatg gactttttca
     1441 gcagcctcct agacccggca ggagccgggg tcacaactgg ccaaatgcgc caaatcaaac
     1501 cgctcgtcgt gcggatgcgc gcccaatgac aggacctgct cggagcgcga gccgtagcga
     1561 aacaccgtga tccctttgag ccccatgcga tggccctcgc ggtacaccgt gtccacgtcc
     1621 ttgactgtgg cggcaaaccg gaggttgacg gtcttgctca ccgcattgtc cacgtgcgcc
     1681 tgaaacgcgg cctgcatgcg gaggtgatcc aggggcgcga tgtcgagcgc gcagcgaaac
     1741 acacgctgca cgtcctccgg cacgccagcc acgccctgca cgctcccggt cttgtgaatt
     1801 accggaacca acgcctgcca cggcacgcct cgcgcgtcca tcgcttcggc aaagagcgat
     1861 gagacctcgg ggagcgcgcg gcccccgagc acgtggctgc gcgtgtaggc gactccgaag
     1921 agcggctcga tgcccgaggt ggtgcccgcg atcaggctga tggtgccggt cggcgcaatg
     1981 gacaggacgg tggcgttacg cacgcggcgc ccgctggccg cgaacaccga ctcgctccat
     2041 cccgggaaca ccccgcgttc ggccgccagc cccgcgctcg ccgcgtcagc ggtttcccga
     2101 agcacccggg cgatctccga gccggtctcg cgggcggcct gactctcgta tgagagcccg
     2161 agacgaatca gggtttccgc cagccccatc acgcccagtc cgattttgcg gtggcggagc
     2221 gccatccgct ccaattccgg gatcggatag cggttggcct ccaccacgtt gtccaagaac
     2281 cgcaccccga ggcgaaccaa ttcgcgcagc ttgtcccagt ccaccgcgta tccggtcgcc
     2341 acgcggcgca ggcagcgcgc caggttgatc gatcccaggt tgcagctctc gtacggcagc
     2401 aacggcactt cgccgcacgg attggtggcg cgaatgctcc cggccgtggg cgtgggattg
     2461 gcgcgttcga tcgcgtccaa gaacacgaca cccgggtcgc ccgtggccca cgcggcctcg
     2521 cacagccgct gatacaattc cggcgccttg cgcgtcgcgg tcacggcgtg gttgcgcggg
     2581 ttgcgcagca cccagtcccc gccccgctcg accgcctcca tgaacgcgtc ggagacgccc
     2641 accgataagt tgaagttgcg caacgcgccg ggctcgcgct tggccgcgat gaaggcttcg
     2701 atctcggggt gatccgcgtt cagtatcccc atgttcgcgc cgcgtcgctt gccgccttgc
     2761 cgcatcgctt cggtggcggc gttgaacacg cgcatgaacg acagggcgcc cgacgccacg
     2821 cccacggcac ctcgcacctg atcgcccgcc ggccggatcg ccgagaaatt aaagcccgtc
     2881 cccccgccgg tctggtgcac caacgccgcc tggcgcagcg tggcgaaaat gccgtcgatg
     2941 ctgtcctcga tcggcaagac aaagcaggcg gacaactggc cgatgggagt gcccgcattc
     3001 atgagggtgg gcgaattggg aaggaattcc agccgggtca tggctgcgag gaatcgctcc
     3061 tcccacgcac cggcttgcgc cgcggacccg aaccgcaact ccgcatcggc aatggcgcgc
     3121 gccacgcggc caaagagctg cgccggggtt tcgtcgatcg cgccgtccgg cgcccggagc
     3181 agatagcgcg ccttgagcgt ctccagcgcg ttcgcgctga gttggggttc ttccagccga
     3241 accatgccgt cctccgcgac ccgatcctcg ggattcgacc ttagcatccc actccgcgat
     3301 ccaggaatca acccttcagc gtccgtggct gtctcgtgcg cgggcggtgc cgggagaacg
     3361 gcaccttgtc gaattacggc cgggatgcgt acaatggcgc catcgtgggt ttccgtccac
     3421 cctgtccccg agaggaggcg catgccgacc gccaaagcca cgccgttcgc ggtaaagacg
     3481 atcctggtac ccacggactt ttccgagggc tcccaggccg cgctcgatta cgcggtgaac
     3541 ctggccaagt cgtttggctc caaaatcgtg ctcctgcacg tgatcgaaac cacgacctat
     3601 accatgaccg aggccttaca actggtaaac gtgtacgagg tggtcaggac cgcggtggag
     3661 ccggtgctgg accagatggt cacagattta cagcagcaga aactctccgt gtcgcgggcc
     3721 gtggcgcaag gggcctcggc ccatgaaatc gtctcgcagg cccgtgaggt cggcgcggat
     3781 ttgatcgtga tggggacgca cggccgacgc ggggtcaatc acctgatggt cggcagcgtg
     3841 gctgagcgcg tggtgcgcac ggccgcctgc ccggtgctga ctgtgcgcca gcccgtgacc
     3901 ggggccggac aaaccaagtg accgccctac gatcagcggg agatacaccg agcacgcgag
     3961 cgctggactt gaacaccatt tatccgcgca gtccgaagca gctcctcggc ggctacgcgc
     4021 accttgcccg gatgatcgac aaggcgcggg ccaaggccgc gggcaccttg ggggaataca
     4081 tctacccttg cccgctggat caagcgttgc tggaattcct gggcctcgac gcggacgcgg
     4141 tgttcgacgt cgccaagcgt ggaaccgacg acgaggtctt gcgttggggc caggaacgcg
     4201 ccacgccccg cagcgcaaag gacatcgcgg cgtggaatca gaccttcttc gcgcgaaagc
     4261 ccacggacga gaagaaactg caatacttca tcgagatcag aaaccgggtc gccccgcacc
     4321 gcaccgacgt gaccacgtgg gtggatttgc tcgatctcga agaaggccgg taaccgccgc
     4381 ccagatactc accgccaaat ccccccttgc ccccctttgt caaagggggg caagggggga
     4441 ttttcttggc aatcgctcaa ctagcgttga ttacggcgtg ctgggcttgc cctgttccac
     4501 gtacggcatc agcggcaacg tctgcgcgtc tttcttgacc accaataccg cgatcgcgcc
     4561 gcgcagtgcg tcggtcagcg agtgcgtgac caatggatac cccccctcgt tctgcgggat
     4621 cacgtccacg gtgtacgcgt cgcccgggcc gaccacgact gtctgcacgc cggtcaggcg
     4681 gttggccgga ttgccgctgg cccacgcgtt ctcccatatc tcgccgatcg ggtggaacgc
     4741 ggagaagtta ttcggtcccg cgttcaccat gtagatgcgg acgcgttcgc cgggcttggc
     4801 ttccaagggc ttgccgccca gaaacggatg gtacttgaac accccgccgt tgaacaccgc
     4861 gtggtcgtac ttgcggtcga acatggcctg cacgtcatcg ggattgagcc acaactcgct
     4921 ctgcaccagc acgtactcgc ggtccgcctt gggcaaggcc ttggcgtcct tggggtcgat
     4981 gatgatcgcg cccatcatgc cgcgcgcgat gtgctggatc attggcccta cgccgcagtg
     5041 atagaaaaag atgccgggtt ttgtcggcgt gaacgtaatc ttgtgcgtct cgccgggctg
     5101 caccggcttg tggttcgtca gaaaatccat ttcccccgcg tggaaatcca tggaatgcgc
     5161 cgcgcggttg gtcttggggt tggtcaacga gaattcaatg gtgtcgcctt cggtcgcccg
     5221 caccaccggc cctggaaact gattgttgaa cgtccacgcg ttgagcgtcg tgcccttgtt
     5281 gtcgacgacc acctgggtct cgaccgcggt aaagtccacc ttcacggtct tggcctctgc
     5341 cgtgccccag ccaaacgcca acgccgcgcc gaccgccacc gtcactccca tcaacgcatt
     5401 cgcgcgccgt cctgtcgtcc tgcaatcgct catctcatcc tccctatgtg agtgggcaca
     5461 tcgcccatcg ttaccatatg gtgccccgca cgaaaacaat atgatcgaag tcatattttg
     5521 gtaaaagtcg tgaacaaagg atccggccgt cacaatcacg gccgcctgcg cgggcgtgac
     5581 gaaatttctt gtcattcccg caaaggcggg aatccagtga ctggcttttc tttgaaaggg
     5641 tgacgaagga accgcgtgga ctacgcgggc gacgcagtca gcgccttgag tgctttcaag
     5701 tccttcacga cgatggatcg ccctgacttg cccagcagtc cgtcgcgcgt gagcttgctg
     5761 atggtccgga tcgtggtttc gagcgcgagg ccggtcatgt ccgcgatgtc ctgacggctc
     5821 agttgcatat cgagcaacag accgccggga atgtccttgc ccacgcgcgc ggccagccgg
     5881 gccagcaacg acgccacgcg ctggtccgcc cgctccaacg caaaggtctt ggccgtctcc
     5941 tgggattcgt tgaggcggtg ccccaggtac cggatgattt ccagcgcggc ctcggggttg
     6001 tccttgagca ggcggaagta cgcctgcttg ccgatcttga gcaccttgac cgggcccatg
     6061 ggcttcgcgc aacccgggtg ttcgcccccg tcaaagaccg cggcttcgca acagaacaga
     6121 tccccgggag ccagcacctt gagcacggcc tccttgccct cgggcgagga tttcacgcat
     6181 ttgaccgtgc ccgagagcag gacgtgaaag gcctcggccg gatcgccctc cttgaagatg
     6241 aactcgtcct tctcgtaccg cacctcggcc atgtgcgcgc tcaagtcctt ccgaccggcc
     6301 tcggtcagcg cggacagaat cgggagatcg agcaccttgg tactcgtggt cagggacgga
     6361 acgtcccctg gacgccgaag atttttcccg cgcccgatca ctcggcctcc ggaatcgtct
     6421 caacgtcccg cagccacacc gccgcgtccg cgtcgctggg cgcgcggtag tcgcttcgcg
     6481 gcgagagcga gccgcccgat ccgaccttgg gcgcgttggc gatgcaactg cgcttgaact
     6541 ggctgtcgtg gaagaaccgc tgcgcgaaca cgcgcaacca gcgcttgatg tcgccgatgg
     6601 agtaggcgca gcgctcggcc tcggggatat cgggccacaa gccttcctgc gcgtcgtgcc
     6661 acgcggacca ggccagaaac gccaccttgg acggccggta cccgaatcgc agcgtgtaat
     6721 acagcgtgaa gtcctggagc gcgtacggcc cgatcacatc ctccgtgcgt tgcgcgggct
     6781 ggctcgcgcc gcgctcgccc ttgcccgccg gcaacaactc cgggctgatt gccgtggcca
     6841 gcacgtcgtc caacaccccg ctgacgtttt cgccgagctg gtgcgtgcgc gacacccatc
     6901 gcaccaggta ctggatcaat gtcttgggca cgctcgcgtt cacgtggtag tgcgccatgt
     6961 ggtcgcccac cccgtacgtg caccacccca gcgccagctc gctcaaatca ctggtgccca
     7021 ccacgatccc gccgcgctga ttggccagcc ggaacaagtg gctggtccgc tccccggcct
     7081 ggacgttctc gaacgtcacg tcgtacacgg gcttgccctg cgcgaacggg tggccgagat
     7141 cctggaacat gcgcagacaa ctgggtttga tgtcgatctc gtgcgcctcg caccccaccg
     7201 ccttcatcaa gcgcttggcc tgatcgagcg tgcggtcgct ggtggcaaag cccggcatgg
     7261 tgtacccgag aatgtgggcg cgcggccgct tcatccgatc catcgcctgc gcgcacacga
     7321 gcagggcctg agtcgaatcg agcccgccgg acacgccgat gacgatcttg gcgtccggcc
     7381 cgatggagcg cagtcgcgtg accagcccct gcacctggat ctcgtacacc tcgcggcatc
     7441 gctcatcccg cagcgccgga tcacccggta cgtacgggaa gcgctcgtag cggcgcgcca
     7501 gcggaatccg gcccgacttc ggcagatccg gcgaacagga aatcgcgcga aacgtggatg
     7561 cggcctcacg gtgccggctc accgcctggc caaagctggt ctgccgcatc cgatcctgcg
     7621 ccaagcggtc caggtccacg tcgccggtga tgagctgcgg cccgtccgcg aaccgcgtgg
     7681 cctcggccaa gggcgcgccg ttctcataaa tcatcccgtg cccgtcccac gccagatccg
     7741 tggtggactc cccgacaccg gccgcgctgt acacgtacgc cgcgatattc cggctggact
     7801 gcccggccac gagctggcgg cggtaatccg ctttgcccac cgtgctgttg gacgcggaga
     7861 gattgatcag caccgcggcc ccggcgagcg ccgcgtacga cgacggcgga atcggcaccc
     7921 agagatcctc gcagatctcc acgtgaaagg tcgctagcgg ttgattgtcg atgtggaaca
     7981 gcaggcggtt gccgaacggc accccgcgct gatcgagcag atcgatcgcg tcccggtgcg
     8041 ccgcgtccgc ggacacgaac tgccgcatct catagaactc gcgataattt ggcaaatacg
     8101 tcttgggcac cacgcccaag atcttgccgc ggtgcaccac cgccgcgcag ttaaacaaga
     8161 gcgcatcgac ttgcagaggc agcccgacaa tgctgatcac gggcagctcg cgcgaggcct
     8221 caacaacctt ggccaacgcc tcgcgacacg catccagcag cgcctgctgg tgaaacaaat
     8281 cttcgcacga ata
//
LOCUS       DBNGTT010000185         6583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3103487, whole genome shotgun sequence.
ACCESSION   DBNGTT010000185 DBNGTT010000000
VERSION     DBNGTT010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3103487"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1950)
                     /locus_tag="AC16BC_04210"
     CDS             complement(283..1950)
                     /locus_tag="AC16BC_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3240853.1"
                     /translation="MASTQSETGVVRKPFQKGTEMTDIELMYAETFKQLEEGSIVDGT
                     VVAVQPEGVVVDVGYKSEGIVPREEFAPDELNALKVGDRFPVYLEEREDADGNMILSK
                     EKADRMKIWGDLERIFEKGDVIEGKVLNKIKGGMMVDIGIKAFLPGSQIDLRPVRDLD
                     ALVGKTFQMKIIKMNHRRSNVVVSRRVVLEETRDRRKQQAMSTLQEGQIIDGQVKNIT
                     DYGAFIDLGGIDGLLHITDMSWGRVAHPSEMFMVGDRVKVMILKYDRETGRISLGVKQ
                     LKPDPWVQVEDKYPVGSRVRGKVVSLVDYGAFVELEPGIEGLVHISEMTWSHEIKHPS
                     KIVTAGDQVEAQVLNVDRKSRKISLGMKQVVANPWIIVETKYPVGSTIEGKVKSLTEF
                     GVFVGLEEGIDGLIHVSDLSWTKRVVHPSDLFKKGQSVKAVVLRVDKDKERLSLGYKQ
                     LTPDPWEREIPQRYQVGQDVSGKVVKVADFGVFLEFEDGVEGLIHVSEMNLEGQRIDE
                     RYKPGDEVTARVIRIDPAERKIALSIREHVGDWGLSEGGDRQTAKRR"
     gene            complement(1996..2664)
                     /locus_tag="AC16BC_04215"
     CDS             complement(1996..2664)
                     /locus_tag="AC16BC_04215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3240854.1"
                     /translation="MLYEVAHPLVSWVARWCCSLEVVGRERVPADGGVIVAANHVSYL
                     DIPIVGCALARRADFLAKDDLFKHPVAGWFFRRLGGVPIRRGTVDRAALAEVERRLAA
                     GRLVVMYPEGTRSPDERLREAKPGVGMLAVRTGAPVVPAYVAGTGEAWPPGAKWLRCR
                     PITVVFGEPMQWRKNEARGDDEPSSKRRYQEVSQEIMGRIAELQREAQARRPRVSEAS
                     AVPR"
     gene            complement(2668..3357)
                     /gene="cmk"
                     /locus_tag="AC16BC_04220"
     CDS             complement(2668..3357)
                     /gene="cmk"
                     /locus_tag="AC16BC_04220"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3240855.1"
                     /translation="MSKRELIVAIDGPSAAGKSTAGTRVAKRLGYLYIESGAFYRALA
                     WKVREQGIDPSDNGALQRLSGTTVIEVVRRPDGARILVDQRDVTDQLRTPDMSRIAAQ
                     IASNPLVREPMLRLQRTLAKDGGVVMEGRDIGTVVFPEADVKFYLDADTRIRGERRYK
                     ELRAKGMDVELQTTIDEIVARDRKDMDRAVAPLRKADDAIAVDSTGLTLDEVVDTMVA
                     AVERRRTAMSR"
     gene            complement(3354..4664)
                     /gene="aroA"
                     /locus_tag="AC16BC_04225"
     CDS             complement(3354..4664)
                     /gene="aroA"
                     /locus_tag="AC16BC_04225"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3240856.1"
                     /translation="MKAAAAKAVVVKPGAPLQGRIEVPGDKSITHRAIMLGGLARGET
                     LIRGYLPADDCLRTVAAMRALGRVIDTVESDDRPALRIAAGTGALREAEDVIDCGNSG
                     TSIRLLTGLVAGLPMCTVLTGDASLRRRPMARVVEPLRAMGAMIVGRDDGRLAPLAVA
                     GRRLKAGEFRLPVASAQVKSAILLAGLGAEGRTAVSEPSASRDHTERMLREFGVPVAT
                     DGGAVSVTGPVNLTGTTVDVPGDISSAAFLIAAALLVPGSELEIGRVGVNPTRTGFLD
                     ILASMGAAVKLGNAREVSGEPTASLTVRTAPLRGATIKGQLVPRAIDEFPILCVLAAF
                     ANGETIVDDAGELRVKESDRIRVMAEELGKLGITVEERPAGMRIVGPQRATGGRCSSH
                     GDHRVAMALAVAALRATGPTTIDDVECVGTSFPGFFELLDRVRR"
     gene            complement(4661..5506)
                     /locus_tag="AC16BC_04230"
     CDS             complement(4661..5506)
                     /locus_tag="AC16BC_04230"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR3240857.1"
                     /translation="MGRLTVVGVGLVGGSVAWGAKQRGLAREVVGVDTDRAASRLAVD
                     QGIVDAVVSPADGVRGADVVVLAVPVGRLEAVAREVAPFVDRATVVTDVGSVKAPVVA
                     NLEPLFARFVGGHPMAGSERSGVAAASADLFAGARCVLTPTPSTEAGAIAAVEGLWSA
                     LGASVVRMEPAVHDDLVAAVSHVPHLVAAALVNAAAHAADGRALTLAAGGFRDTTRVA
                     GGSPDLWRDICLMNRGPVLAMLKSYLKEVESIRDAIERGDDGALLNALARARAARDRV
                     PGGEA"
     gene            complement(5526..6539)
                     /gene="aroF"
                     /locus_tag="AC16BC_04235"
     CDS             complement(5526..6539)
                     /gene="aroF"
                     /locus_tag="AC16BC_04235"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3240858.1"
                     /translation="MLVVMRQDATAEQLNGVVAAIEARGLQAHAIPGAERTAIGITGN
                     QGPLEPAIFESLPGVLEAIRVSHPYKLVSREVKREDSVVRIGGVPIGGGALVVIGGPC
                     AVESREQTLTVARGIKAAGGHILRGGAFKPRTSPYAFQGLGEEGLRILALAREETGLP
                     IVTEAVDTESLELVERYADAIQIGARNMQNFRLLTEIGKQRKPVLLKRGLSATIKEFL
                     LSAEYIMAGGNHNVMLCERGIRTFETQTRNTLDLSAVPLIQKLSHLPVVVDPSHATGR
                     WDLVVPLARAAVAAGCDGLLVEVHGNPEEALCDGEESLTPAKFTAMMDELRKVAQAVG
                     REI"
BASE COUNT         1050 a         2522 c         1944 g         1067 t
ORIGIN      
        1 gtgcggctgc cgttccacca acaacgcggt gaaatacgtg ccgagaaaaa acgcggcggc
       61 caggatcaag aaaacgataa aaccggtaac cagcgggcgt ttggccatga ctatctttga
      121 cccttaccgc ccttccgact ccgaaggagt cggaggctgt ccgggaaccg gccggatgca
      181 aggcggagcg agcgcccgca ccgcagcgta ctgggtcgta cgtgaggagc ggacgcgcag
      241 cgacaacgcc gcagacggcc gttcatggac agcctcccgc cactaacgcc gcttggcggt
      301 ctggcggtcg ccgccttccg agagccccca gtcgcccaca tgctcccgaa tgctcagcgc
      361 aatcttgcgc tccgccggat cgattcggat cacgcgcgcc gtcacctcgt cgccgggctt
      421 gtaccgctcg tcgatgcgct ggccttccag gttcatctcg ctcacgtgga tcaatccctc
      481 gacgccgtcc tcgaattcca ggaacacgcc gaagtccgcc accttcacga ccttgccgga
      541 gacgtcctga cccacctgat agcgctgcgg aatctcgcgc tcccacggat ccggcgtcaa
      601 ctgcttgtaa cccagcgaca gacgctcctt gtccttgtcc acgcgcagca ccacggcctt
      661 gaccgactgc cccttcttaa agagatccga cggatgcacc acgcgcttgg tccacgagag
      721 gtcggagacg tggatcagcc cgtcgatacc ctcttccagg cccacgaaca cgccgaattc
      781 ggtcaggctc ttgaccttgc cctcgatggt ggagcccacc gggtacttgg tctccacgat
      841 gatccaagga ttcgccacca cctgcttcat gccgagcgag atcttgcggc tcttgcgatc
      901 cacgttgagc acctgcgcct cgacctgatc gcccgccgtc acgatcttgg acgggtgctt
      961 gatctcgtgg gaccacgtca tttccgagat atggaccagg ccctcgatcc cgggctccag
     1021 ttccacgaac gcgccgtagt ccaccaggct gaccaccttg ccgcgcacgc gcgagcccac
     1081 cgggtacttg tcctcgacct gcacccacgg atccggcttg agctgcttga cccccaagga
     1141 aatgcgcccg gtttcccggt cgtacttgag gatcatgacc ttgacgcgat cgcccaccat
     1201 gaacatttcg gacgggtgcg ccacgcggcc ccacgacatg tcggtgatgt ggagcaaccc
     1261 gtcgatgccg cccaggtcga tgaacgcccc gtagtccgtg atgttcttga cctgcccgtc
     1321 gatgatctgg ccttcctgca acgtggacat ggcctgctgc ttgcggcgat cgcgcgtctc
     1381 ctcgagcacc acgcggcgcg acaccaccac gttgctgcgg cggtggttca tcttgatgat
     1441 cttcatctgg aaggtcttgc cgaccaacgc gtccagatcc cgcaccggac gaaggtcgat
     1501 ctgcgagccg ggcaaaaacg ccttgatccc gatgtcgacc atcatcccgc ccttgatctt
     1561 gttgaggacc ttgccttcga tcacgtcgcc cttctcgaag atccgctcca gatcgcccca
     1621 gatcttcatg cgatcggcct tctctttcga gaggatcatg ttgccgtcgg cgtcttcccg
     1681 ttcctccagg tacaccggga accggtcgcc caccttgagc gcgttgagtt catccggagc
     1741 gaactcctcg cgcggaacga tgccttcgga tttatatccc acgtcgacga ccacgccctc
     1801 gggctgcacg gccacgacgg tgccatccac gatgctgcct tcctccaatt gcttgaaggt
     1861 ttcggcgtac atcaattcga tgtccgtcat ttccgtgccc ttttggaagg gcttgcgaac
     1921 gaccccggtt tccgactgcg tgctcgccat acgtctacat cctctcttcg ctgcgatact
     1981 gcggtgaagg gtgaattacc gtggaaccgc cgaagcctcc gacacccgcg ggcgcctggc
     2041 ttgcgcctcg cgctggagct ccgcgatccg tcccatgatc tcctgactga cctcctggta
     2101 ccggcgcttg gacgacggct cgtcgtcgcc gcgcgcttcg ttcttccgcc actgcatcgg
     2161 ttcgccgaac accaccgtga tcggccgaca ccgcaaccac ttcgctccgg gcggccaagc
     2221 ctcgcccgtg cccgccacgt aggcgggcac caccggtgcg ccggtgcgca ccgccaacat
     2281 cccgacgccc ggcttggcct cgcgcaaccg ctcgtcggga ctgcgcgtgc cttccggata
     2341 catcacgacc agccgcccgg ccgccaaccg ccgttccact tcggcgagcg ccgcgcgatc
     2401 gacggttccc cgtcggatcg gcaccccgcc gagccggcgg aagaaccatc ccgccacggg
     2461 gtgtttgaac aaatcgtctt tcgccagaaa atccgcccgt ctggcgagcg cacatcccac
     2521 gatcggaatg tcgaggtaac tgacgtgatt ggcggcgacg atcaccccgc cgtcggccgg
     2581 gacccgttca cgtcccacca cctccagcga gcagcaccac cgcgcgaccc atgagaccag
     2641 agggtgcgcg acctcgtaca gcacaactta ccgcgacatc gcggtccgcc ggcgttcgac
     2701 ggccgcgacc attgtatcga ccacctcatc caaggtcaag cccgtcgagt ccacggcgat
     2761 cgcgtcgtcg gccttgcgca agggcgcgac cgcgcggtcc atgtccttgc ggtcgcgcgc
     2821 cacgatctcg tcgatcgtgg tctgcaactc cacgtccatg cctttggcgc gcagctcctt
     2881 gtaccgccgc tccccgcgaa tccgcgtgtc cgcgtccaga tagaacttga cgtcggcctc
     2941 cggaaacacc acggtgccga tgtcgcgccc ttccatcacc accccgccgt ccttggccaa
     3001 cgtgcgctgc aggcgcaaca tgggctcgcg caccagggga ttggacgcga tctgcgccgc
     3061 gatccggctc atgtccggcg tgcgcaactg gtcggtcacg tcgcgctggt ccaccaggat
     3121 cctggcgccg tccggacgtc ggaccacctc gatcacggtg gtcccgctca ggcgttgcag
     3181 cgcgccgttg tccgacggat cgatgccctg ttcgcggacc ttccacgcca gcgcccgata
     3241 gaaggccccg ctctcgatgt agagataccc caagcgtttg gccacccgcg tgcccgcggt
     3301 gctcttgccc gcggccgagg ggccgtcgat cgccacgatc aactcccgct tactcatcgt
     3361 ctcacccggt cgagcaattc gaaaaacccg gggaacgacg tccccacgca ctccacgtcg
     3421 tcgatcgtgg tcggcccggt cgcccgcaac gcggccacgg ccaacgccat cgccacccgg
     3481 tggtctccgt ggctggagca gcggccgccg gtcgcgcgtt gcggccccac gatccgcatg
     3541 ccggccgggc gttcctcgac cgtgatgccc agtttcccga gttcctccgc catcacccgg
     3601 attcgatcgc tctccttgac ccgcagttcg cccgcgtcgt ccacgatcgt ttccccgttc
     3661 gcgaacgccg ccaacacgca cagaatcggg aactcatcga tcgcgcgcgg caccaactgg
     3721 cccttgatcg tcgcgccgcg aaggggcgcg gtccgcacgg tcaacgacgc ggtgggctcg
     3781 ccggacactt cgcgggcgtt gccgagcttc acggccgcgc ccatgctcgc gagaatgtcg
     3841 aggaacccgg tccgcgtcgg gttcacgcct acccgaccga tctccagctc ggaccccggc
     3901 accaacaacg ccgccgcgat caagaacgcg gcggacgaaa tatcgcccgg cacgtccacg
     3961 gtggtgccgg tcagattcac cggtccggtc accgagacgg cgccgccgtc cgtggccacg
     4021 ggcacgccga actcgcgcaa catgcgttcg gtatgatcgc gcgacgccga cggctcggac
     4081 accgcggtgc gcccctcggc cccgagacct gccagcagga tcgcggactt gacctgcgcg
     4141 ctggccaccg gcaaccggaa ctccccggcc ttgagccggc ggcccgccac ggccagcggc
     4201 gccagtcggc cgtcgtcccg gcccacgatc atcgcgccca tcgcgcgcag cggctccacc
     4261 acccgggcca tgggccgccg ccggagcgac gcgtcgccgg tcaacaccgt gcacatgggg
     4321 agtcccgcga ccaacccggt caacaaccgg atgctggtgc ccgagttccc gcagtcgatc
     4381 acgtcctcgg cttcccgcag cgccccggtc ccggccgcga tccgcagcgc cgggcgatcg
     4441 tcggactcca cggtgtcgat gacgcgaccc aacgcccgca tcgcggccac ggtgcgcaga
     4501 caatcgtccg cgggcaaata gccgcgaatc aacgtttccc cgcgcgccag cccgcccagc
     4561 atgatcgcgc ggtgcgtgat cgacttgtcc cccggaacct cgatccgtcc ctggagggga
     4621 gcgccgggtt tgacgaccac ggccttggcc gccgcggcct tcacgcctcg cctcccggca
     4681 cccggtcgcg cgccgcgcgc gcgcgcgcca acgcgttgag caacgcgccg tcgtcgccgc
     4741 gctcgatcgc gtcgcggatc gactcgactt ccttcagata ggacttcagc atcgccaaca
     4801 ccggtccccg gttcatcaga caaatgtccc gccacaggtc cggcgatccg cccgcgaccc
     4861 gtgtcgtatc gcgaaacccg cccgccgcga gcgtcagcgc ccgtccgtcc gccgcgtgcg
     4921 cggccgcgtt gaccaacgcg gccgcaacca gatgcggcac gtggctcacc gcggcgacca
     4981 agtcgtcgtg cacggccggc tccatgcgga ccaccgacgc gcccaacgcg gaccacaacc
     5041 cctccaccgc cgcgatcgcg cccgcctccg tgctcggcgt gggtgtcaac acgcatcgcg
     5101 cgcccgcgaa caggtcggcc gacgccgcgg ccaccccgga gcgctcgctc ccggccatcg
     5161 ggtgtccacc cacgaaccgc gcgaagagcg gctccaggtt cgcgaccacc ggggccttca
     5221 cgctccccac gtcggtcacg accgtggcac ggtccacgaa cggcgcgacc tcgcgcgcaa
     5281 cggcttcgag tcgccccacc ggaaccgcca gcaccacgac gtccgcgccg cgcacgccgt
     5341 cggccggcga aaccacggcg tccacgatcc cctgatcgac cgcgaggcgg ctcgcggcgc
     5401 ggtcggtatc gacgccgacg acttcccgcg ccagaccgcg ctgcttggcg ccccaggcca
     5461 ccgacccgcc gaccagaccg accccgacca cggtgaggcg gcccatccgc gccgtgcgcg
     5521 agatgtcaga tctcccggcc cacggcctgc gccaccttgc gcaattcgtc catcatcgcg
     5581 gtgaacttcg ccggggtcag cgactcctcg ccgtcgcaca aggcctcttc cgggttgccg
     5641 tgcacctcga ccaacaatcc gtcgcacccc gcggccaccg cggcgcgcgc caacggcacg
     5701 accaagtccc agcgccccgt ggcgtggctc ggatccacca ccaccggcag gtgcgacagc
     5761 ttctggatga gcggcaccgc ggacaaatcg agcgtgttgc gcgtctgggt ttcgaacgtc
     5821 ctgatcccgc gctcgcacaa catgacgttg tgattgccgc ccgccatgat gtactccgcg
     5881 gacaacaaga actccttgat ggtggcggac agtccgcgct tgagcaggac cggtttgcgc
     5941 tgtttgccga tctcggtgag cagccggaag ttctgcatgt tccgagcgcc gatctggatc
     6001 gcgtccgcgt agcgctcgac gagctcgagc gactccgtgt ccacggcttc ggtcacgatc
     6061 ggtagccccg tctcctcgcg cgccagggcc aggatccgga ggccctcctc gcccaacccc
     6121 tggaacgcgt acgggctcgt gcgcggtttg aacgccccgc cgcggaggat gtgcccgccg
     6181 gcggccttga tcccgcgggc caccgtcagc gtctgttctc gcgattccac ggcgcagggg
     6241 ccgccgatga ccacgagcgc gcccccgccg atcggaacgc cgccgatccg gaccacgctg
     6301 tcctcgcgct tcacctcgcg agagacgagc ttgtaggggt gcgagacgcg gatggcttcg
     6361 aggacccccg ggaggctctc gaagatcgcc ggctccagcg gcccctgatt tcccgtaatc
     6421 ccgatcgcgg tgcgctcggc ccccgggatc gcgtgggcct gcaggccgcg cgcctcgatg
     6481 gccgcgacga caccgttcaa ctgctccgcc gtcgcgtcct gacgcatcac cacgagcacg
     6541 ggagacctcc gccgctatct taaggcgaag cggcggcttc ggc
//
LOCUS       DBNGTT010000186         4363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1058989, whole genome shotgun sequence.
ACCESSION   DBNGTT010000186 DBNGTT010000000
VERSION     DBNGTT010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1058989"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..369
                     /locus_tag="AC16BC_04240"
     CDS             10..369
                     /locus_tag="AC16BC_04240"
                     /inference="COORDINATES: protein motif:HMM:NF046205.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6946 family protein"
                     /protein_id="HIR3240859.1"
                     /translation="MKDKRAEESAGQHDRLAYLQSLLGVPQLDDVIRYQLLHRTASVI
                     LTAQEFHAHVAVMIVQSFGKRSSLREDFDRFANALRAEDLPGGMRIVRSFKQPRLFLG
                     WCNGDPKFLQVELPSAV"
     gene            450..866
                     /locus_tag="AC16BC_04245"
     CDS             450..866
                     /locus_tag="AC16BC_04245"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HIR3240860.1"
                     /translation="MGQLLKAGLKKVGAGLLYGVGFGVSAGLIMNFIVDRMTPSFDPT
                     PIDKVSITGHEEVKRAGEVFFLGTLENHASDPVRIVKIQVDLFDKQGKLVEICDEHVR
                     GFLKSTESVNFKVSCGSTGKPVVEHDSYKIHPVGLL"
     gene            1556..2197
                     /locus_tag="AC16BC_04250"
     CDS             1556..2197
                     /locus_tag="AC16BC_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240861.1"
                     /translation="MVKKIKKKSPAGSSGWRGVPLALVVTALAGPRREGRDVLRAFSP
                     AWRLREIFLDHFSLEVVSTDRPLTLAEFMAWPGLADPAWAFKSAGGHGVVLTKAPMEE
                     TSEGPRLGVVPIVVRLMASDLDRLSDLWADDFPEVARVALTAAFTEAMNLIPGYDRAV
                     WSGPRPARDLNALINHLNALVADECAMAGVDAPRRFPLGAEQRIAAIPSVVTL"
     gene            complement(2201..3274)
                     /locus_tag="AC16BC_04255"
     CDS             complement(2201..3274)
                     /locus_tag="AC16BC_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3240862.1"
                     /translation="MTPDAASILASLHEERTSAFLYRSMEAVESDSLRAALFAKLRHA
                     SERQASLWEERLRALGQPVPEFHPPVRARLVARLLRLLGPRPMLPVLAAMKVRGLSIY
                     RSGPVAPGSADADAAFAAATAPTAETWHRAQQGGGALRAAVFGVNDGLVSNASLIVGV
                     AAAGADPHGVVIAGFSGLLAGSLSMATGEYVSVKTQRELLEHQIALEQHELDTMPEEE
                     IDELAAIYEAKGLSAEAARSVASRLIADPKQGLDTLAREELGLNPSDLVSPVSAALAS
                     FASFAAGAVIPLVPFLLPTPQPGLWGSLIAMEAGLLIVGGLMSLFTGRGVLWSAFRMA
                     LLGSAAAAATFVIGRLLGVAVAG"
     gene            complement(3445..>4363)
                     /locus_tag="AC16BC_04260"
     CDS             complement(3445..>4363)
                     /locus_tag="AC16BC_04260"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3240863.1"
                     /translation="YAHEKGVVVLVDEAHGPHLKFHPDLPASALEAGADLCVQSTHKI
                     IGGMTQASMLHARSARVNLSMVSNVLRFVQTTSPSYILMASLDLARMQMATEGEKLLD
                     KAIRLAQDARARINKIPGLFCLGKDNVAGGLFAQMGDLDVTKLTITVTDLGLSGYQVS
                     QLLNTKYHIQVEMADPFHVLVIVSIGDRQDDLRRLVDALRDIAKEYGTRSGPSPLASE
                     PLPAFGHDAVMTPREAFFADQDYIPLDSAVGAVSSEIVTVYPPGIPVLVPGEIITAEA
                     VTYLKRMLVLGGTVDGLDETNSWIGVVKR"
BASE COUNT          767 a         1310 c         1458 g          828 t
ORIGIN      
        1 ggccctctcg tgaaggataa acgagctgag gaatcggccg gtcagcatga ccgcctcgcg
       61 tacctccaat ctctcctcgg ggttccacag ctggatgacg ttattcggta tcaactgctt
      121 caccgcactg cctcggtcat ccttacagca caagagtttc atgcgcacgt cgcagtaatg
      181 atcgttcagt cttttgggaa gagaagttct cttcgcgagg actttgatcg cttcgctaac
      241 gcactgcgtg ccgaggacct ccccggaggt atgagaatcg tccgctcatt caaacagccg
      301 cggctttttc tgggctggtg taacggtgac cccaagttcc ttcaggtgga gctgcccagt
      361 gcggtctaac acggcgctgg agatgacgcg cgaatagcgc gcgcccctca gcgctggtcg
      421 ttagcgctca agttcactga ggaggcactg tggggcaact tcttaaggcg gggctgaaga
      481 aggtcggggc tggcctcctt tatggcgttg gctttggagt gtcggcagga cttattatga
      541 attttatcgt tgataggatg acgccttctt tcgacccgac accgattgat aaggtgagta
      601 tcacgggaca cgaggaggta aagagagccg gggaagtatt cttcctcgga acactcgaga
      661 accacgcttc agatcctgtg cgtatcgtga aaatccaagt ggacttgttc gacaagcagg
      721 ggaaactcgt cgaaatatgc gacgaacatg ttcgcggttt tctaaagtcg acagagtcgg
      781 tgaatttcaa agtctcctgt ggctctacgg gaaagcctgt cgttgagcac gacagctaca
      841 aaatccatcc tgtggggctg ctgtgatcgt ggaaacgcag tcggggtcgc atctttaatc
      901 tttaggcgcg agggagtgta cggcaaatcc ccccttcccc ccctttgtca aaggggggaa
      961 gattctaaac aggcgaagtt ctggaatcga gatttgtttg tatggcgcgc tacgcagtgt
     1021 ccgtatctcg ctgttcgctt gacttgctcc agatgctttc ctagaatccg gccgtgcgat
     1081 ttccgatggc tcgtcgacca aaagcgccag cgtcaggcgg cgcgccgatg aagcagggcg
     1141 cggctcctgg gaccgcaccg cgcggcgcgc ggccggccaa tcccgcggca gcgtcacaac
     1201 gtcctgagca gttccaagag ttgcgggcct cggcgctgta ttgtcctaag tgccgagagg
     1261 ccacgcccac gcgagaagtg ctcttgttgg tccttcccga tggcggcagt ctccttgatt
     1321 accgctgcgc gcgttgcggt acctcgaccg gctcgcgtac ggcgaagccg gcctctgaca
     1381 ttcgcgtctt tcgcggttaa tccgacccag cgcgtcactg aataaaagcg gaagcgtggt
     1441 tcgacaggct caccacgaac ggcgattgaa tcggaacgtc aacacttcat ccgttcgtcc
     1501 tgagctcgtc gaaggacctc cgcaggtttc tgagtgattc cctaggtttc tctccgtggt
     1561 caaaaagatc aagaaaaaat ctccggccgg ctcgtccggc tggcggggcg ttccgctggc
     1621 gttggtggtc acggcgttgg cgggaccgcg gcgcgagggg cgcgatgtgt tgcgggcgtt
     1681 ttcgccggcg tggcggctgc gggaaatctt tctggatcat ttcagtctgg aggttgtctc
     1741 cacggatcgg ccgctcacgc tcgccgagtt catggcatgg cccggtctgg cggaccccgc
     1801 gtgggcgttc aagtccgcgg gcggacacgg agtggtgttg accaaggcgc cgatggagga
     1861 gacgagcgag gggccgcgac ttggtgtggt gccgatcgtg gttcgcctca tggcgtctga
     1921 tctggaccga ctgtccgacc tgtgggccga cgactttccg gaggtggcgc gggtggcgtt
     1981 gaccgcggcg ttcaccgagg ccatgaatct gatcccgggg tatgatcgcg cggtgtggag
     2041 cggcccgcgg cccgcgcgcg acctcaacgc gctcatcaat cacctcaacg cactggtggc
     2101 ggatgagtgc gcgatggcgg gcgtggatgc tccgcggcgg tttccgctcg gggctgagca
     2161 gcggatcgcc gcgattccgt cggtcgtgac gttgtagcgg tcagcccgcg accgcgacgc
     2221 cgagcaatcg gccgatcaca aatgtcgcgg ccgcggcggc cgagcccagc agggccatcc
     2281 gaaacgcgct ccagagcacg ccccgtccgg tgaacaggct catcaagccg ccgacgatca
     2341 ataagcccgc ttccatcgcg atgagcgagc cccacaggcc tggttgcggg gtggggagca
     2401 gaaacgggac gagcggaatg acggcgccgg ccgcgaagct cgcgaacgaa gccaatgccg
     2461 cggagacggg cgagaccaag tcgctggggt tcaggcccaa ttcttcgcgc gccagggtgt
     2521 cgagtccttg tttggggtcg gcgatcaggc gcgaggccac cgatcgcgcg gcctccgcgg
     2581 acaagccttt ggcctcgtag atcgcggcga gttcgtcgat ttcttcctcc ggcatagtgt
     2641 cgagttcgtg ttgctccagc gcgatctggt gttccaacag ttcgcgctgc gttttgaccg
     2701 aaacgtattc gccggtggcc atggacaggc tgcccgcgag caaccccgaa aatcccgcga
     2761 tcaccacgcc gtgcgggtcc gcgccagccg cggccacgcc cacgatcaaa ctcgcgttgg
     2821 acaccaaccc gtcgttgacc ccgaacactg cggcccgcag cgcgccgccg ccctgctggg
     2881 cccggtgcca ggtctcggcc gtgggagcgg tggccgcggc gaacgccgcg tcggcgtccg
     2941 cgctgcccgg cgccactggg cctgatcgat aaatcgacag cccgcggacc ttcatcgcgg
     3001 ccaggaccgg gagcatgggt cgaggaccca ggaggcgaag gagtcgcgcg accaggcgcg
     3061 cgcgaaccgg tgggtggaac tcggggaccg gttggccgag cgcgcggaga cgctcctccc
     3121 acaacgaggc ttgccgttcc gacgcgtgtc ggagtttggc aaagagcgcg gcgcgcaggc
     3181 tgtcggattc cacggcttcc atcgaacgat ataagaacgc ggacgtgcgc tcttcgtgga
     3241 gcgaggcgag aatgctggcg gcgtctggcg tcacggcggg tcctgtgttg ccaggaggcc
     3301 gtctatgtga gcccagttgg agcctgttca ggaggggcca gatgccaggc ggagggagga
     3361 ccttgcgcgc ggagcgtact gggtcgtacg tgagcacgca aggccgaccg acaacgccgc
     3421 agatggcccc tcatggacgg gctcctaccg cttgaccacg ccgatccaac tgttggtctc
     3481 gtccagaccg tccacggtcc caccgagcac caacatgcgt ttcaagtagg tcacggcttc
     3541 cgcggtgatg atctcgccgg gcaccagcac cggaatgccg ggcgggtaca cggtgacgat
     3601 ctcggaggag accgcgccga ccgcggaatc cagggggatg tagtcctggt ccgcgaagaa
     3661 cgcctcgcgc ggggtcatga ccgcgtcgtg cccgaacgcg ggcaatggtt cggaggcgag
     3721 cggtgacggg cccgacctgg tgccgtattc cttggcgatg tcgcggagcg cgtcgaccag
     3781 gcgcctcagg tcgtcctggc ggtcgccgat gctcacgatc accaggacgt ggaacgggtc
     3841 cgccatttcg acctggatgt ggtacttggt gttgagcagt tgggagactt ggtagcccga
     3901 gagcccgagg tcggtcacgg tgatggtcaa cttggtgacg tcgaggtctc ccatctgcgc
     3961 gaacagaccg cccgcgacgt tgtctttgcc caggcaaaag agcccgggga tcttgttgat
     4021 gcgggcgcgc gcatcctgcg ccaagcggat ggccttgtcc aggagtttct cgccttccgt
     4081 ggccatttgc atgcgcgcca gatcgagcga cgccataaga atgtaggacg ggctggtggt
     4141 ctgcacgaac cgcagcacgt tgctcaccat cgagaggttc acgcgggccg aacgggcgtg
     4201 gagcatggac gcctgggtca tgccgccgat gatcttgtgg gtgctctgca cgcacaggtc
     4261 ggctccggct tccagcgccg aggccggcag gtccgggtgg aacttcaagt gcggaccgtg
     4321 ggcctcgtcc accagcacca ccacgccctt ctcatgggcg tag
//
LOCUS       DBNGTT010000187         1892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1442860, whole genome shotgun sequence.
ACCESSION   DBNGTT010000187 DBNGTT010000000
VERSION     DBNGTT010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1442860"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="AC16BC_04265"
     CDS             <1..698
                     /locus_tag="AC16BC_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240864.1"
                     /translation="EYDNLVADLLFSRTLSFHGGKSNRHESITPETYQTIYRRLDAAS
                     ALDPYFVDPYYFGQSVLTQGANMPREANSLLDRSRRYRTDDWVMPFFMGFNEFYFLGD
                     NATAAQYLMESAHRPGSPPLVGLLAARLAARGGTTDVSVAFLDQFAEQVEDPVSKQMV
                     RQRADALRGIAILEHAIARYREMFGAPPSDLRVIVDRGVLLELPTDPYGGTYYLTSQG
                     TVWTTSDLRPIEK"
     gene            695..1222
                     /locus_tag="AC16BC_04270"
     CDS             695..1222
                     /locus_tag="AC16BC_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR3240865.1"
                     /translation="MRLLAPHTRVTCWNGGFTLVELLIVVAILGILAAIGIPQYTQYR
                     ARANNTSALTDIQTVRTAEHALFAEKQQYASTAATGCVGDPICTGTINFGIAGVTDVV
                     LRPGTALEAIGTVTSFTAATKSVSGDRVYCVDSDVSFIRYTNAPINTPLGAPFRAPTP
                     NAGADDCIATFPNVQ"
     gene            1278..>1892
                     /locus_tag="AC16BC_04275"
     CDS             1278..>1892
                     /locus_tag="AC16BC_04275"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3240866.1"
                     /translation="MRVPLLDLKLQYQKIKPDVLQVIHQVCEEQAFILGSQVEAFEQD
                     VAKYLGAAYAIGVTSGTDALLLALMAAGVKPGDEVITTPYTFFATAGSISRVGAVPVF
                     VDIDPATYCLDVAKLGDALTPKAKAVIPVHLYGRPMDLDPVLELAEAEDLLVIEDAAQ
                     AIGATINGKRAGTFGAFGAFSFFPSKNLGGFGDGGLVTTNNPERA"
BASE COUNT          358 a          551 c          593 g          390 t
ORIGIN      
        1 tggaatacga caaccttgtc gcggatctgc ttttttccag aacattgagc ttccacggtg
       61 ggaaatcaaa tcgacatgaa tcgatcactc ccgagacgta tcaaaccatt tatcgcagac
      121 tcgacgcggc atctgccctc gatccgtact tcgttgatcc gtattacttc ggacagtccg
      181 tcctgacgca gggggcgaac atgccgcgcg aggcgaatag ccttctggat cgtagccgcc
      241 gctaccggac cgacgattgg gtgatgccgt ttttcatggg gttcaacgag ttctattttc
      301 tcggcgacaa cgccacggcc gcgcagtacc tgatggagtc cgcccacagg ccgggcagcc
      361 cgccgttggt cgggctgttg gccgcgcggc tggctgcgag gggaggcaca acggatgtct
      421 cggttgcgtt tctcgatcag ttcgcggaac aggtcgagga tcctgtgtca aagcagatgg
      481 tccggcagcg cgcggacgcc ctccggggaa tcgcgattct ggagcacgcc atcgcgcggt
      541 atcgtgagat gttcggggcg ccgccgtccg acctgcgcgt aatagtcgac cgcggcgtgc
      601 tcctggaact gccgaccgac ccctacggcg ggacgtatta tctcacgagc caagggaccg
      661 tctggactac gagcgatctg aggccgatag aaaaatgagg cttcttgcgc ctcatacacg
      721 agtaacatgc tggaatggtg gattcacgct cgtagaatta ttgattgtgg tcgcgatcct
      781 gggaattctg gccgcgatcg gtattccaca gtacacccag tatcgggcgc gggcgaacaa
      841 cacgagcgcg ctgaccgata ttcagacggt tcggaccgcg gaacacgcgc tgtttgccga
      901 aaagcagcaa tacgcatcga cggcggcgac tgggtgcgtg ggcgatccaa tttgcaccgg
      961 cacgatcaac ttcgggattg ccggcgtgac cgacgtggtc cttcggccgg gcacggcgtt
     1021 ggaggccatt ggcaccgtga cgtcgtttac cgcggccacg aagagcgtca gcggagatcg
     1081 tgtctattgc gtggactccg acgtgtcatt catcaggtac accaacgcac cgatcaacac
     1141 gccattgggc gcgcccttcc gggctcctac gccgaacgct ggcgcagatg attgcatcgc
     1201 gaccttcccg aacgtccagt aactcagctc gcggttcggt ttgacacccc cacccccggt
     1261 tgttacaatc cgccccgatg cgtgttcccc tcctggattt aaagctccag tatcagaaaa
     1321 ttaaaccgga cgtcctgcaa gtcattcatc aggtgtgcga ggagcaggcg tttatcctcg
     1381 gatcgcaggt ggaggcgttc gagcaggacg tcgccaagta cctgggcgcc gcctacgcaa
     1441 tcggcgtgac gtcggggacc gacgcgttgt tgctggcgtt gatggcggcc ggcgtgaagc
     1501 ccggggacga agttatcacc acgccgtaca cgttttttgc gactgcgggt tcgatttctc
     1561 gtgtgggcgc cgtgccggtt tttgtggata tcgatcccgc gacgtattgt ctggacgtgg
     1621 ccaagctcgg cgatgcgctc acgccgaagg ccaaggcggt gattccggtg cacttgtacg
     1681 gcaggccgat ggatcttgat cccgtgctcg aattggccga agccgaagac cttctcgtga
     1741 ttgaagatgc ggcacaggcg atcggcgcga cgatcaacgg gaaacgcgcg ggcactttcg
     1801 gcgcgttcgg ggcgttcagt tttttcccgt cgaagaacct cgggggcttc ggtgacggcg
     1861 gcttggtgac cacgaacaat cccgagcggg cg
//
LOCUS       DBNGTT010000188         4431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_932581, whole genome shotgun sequence.
ACCESSION   DBNGTT010000188 DBNGTT010000000
VERSION     DBNGTT010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_932581"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..656)
                     /locus_tag="AC16BC_04280"
     CDS             complement(207..656)
                     /locus_tag="AC16BC_04280"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR3240867.1"
                     /translation="MLRTIQEFFDTRIRSTSGEPASTDRALQLATAALMVEVSRADHE
                     IKDEERRVIGEAVQKTFGLSEHDTEVLLRLAEEEAQTATSTHQFTQLIDKHFPMEQKI
                     HIVELLWQVAFADAYKDRHEEHLIRRIADLLHVSHREFIDAKLRARG"
     gene            818..1978
                     /locus_tag="AC16BC_04285"
     CDS             818..1978
                     /locus_tag="AC16BC_04285"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR3240868.1"
                     /translation="MPAPSSSFWLRLARPIIGLSPMDGVTDAAFRLIAARHGRPDVMM
                     TEFTHVEGICRAIPAVLRHLDYDPAERPVVAQVFGSEPAAFYKAAQVVAELGFDGLDI
                     NMGCPDKAVAAHGCGAALIQTPERAQAIIRAAQAGVRDWAAGRDLASVGLPDETVEAV
                     RRMAMGAGATDRVRRLIPVSVKTRLGYDRVVIDSWINVLLETEPAAVTIHGRTLEQRY
                     RGEADWDAIARAAELGRHTGTLVIGNGDLRSPADVTRRVLQTGVNGVLIGRAALGNPW
                     IFRATDEIRAACLDSEIHAPVAPVVDARERWAVAREHAAAFDRVRGRLAFAVMRKHLG
                     WYVRGNEDRPGLRARLMQVHDLAELDAVLADSAPWAFTTAEDPAPIASPTCV"
     gene            1975..2547
                     /locus_tag="AC16BC_04290"
     CDS             1975..2547
                     /locus_tag="AC16BC_04290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR3240869.1"
                     /translation="MILASTSPRRRELLARLGVPFEVASPKFEEVIDPLASPETQAEQ
                     FALGKARSIVATDAVVLGSDTLVVVDRTILGKPTDAADARRMLRLLCGRSHRVITAVA
                     VVAPGQPDHVFLDEAVVRMRPADDTEIAAYVSTGEPMDKAGAYAAQGAGARFIESITG
                     DETTVIGLPLRRLTEVLRKVGIRIPAEAPV"
     gene            complement(2722..3096)
                     /locus_tag="AC16BC_04295"
     CDS             complement(2722..3096)
                     /locus_tag="AC16BC_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240870.1"
                     /translation="MDSPVLDHVERGVLGHCVEDYCGSSELLWAVQRLTPGLSNRAVL
                     VRRGLDVLERLLTEHLIAAYVKSSDPATPFAPVSDGIDELLAVVEREHVVSPETPHEQ
                     RYWFTATPAGVTRYFDAQQQAS"
     gene            complement(3106..>4431)
                     /locus_tag="AC16BC_04300"
     CDS             complement(3106..>4431)
                     /locus_tag="AC16BC_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3240871.1"
                     /translation="EADRLCRTFTRVKGRVAYVNGAGEPDVAFEEVGTPTYAGLDRSR
                     YLSIVEVPNPMHRLWSDGRWNKLTVAHGCYWKRCTFCDVDLDYIKRYDPASAAVLVDR
                     IDALIAETGETGFHLVDEAAPPARLVDLALALLERGRVVSWWGNIRFETAFTPDVCRL
                     LAASGCIAVSGGLETASDRLLDLMDKGVTVAQAARTARAFTRAGIMVHAYLMYGFPTE
                     TPQETIDALERVRQLFDADLIQSAYWHRFTATRHSPVGRRPSAFGISIEPLPARAFAR
                     NDLVHQDPRSGNLEALGAGLARAVQHFMHGLGLDRDVRTWFEIRVPRPRVSAEWVQRA
                     QRIAEPAILDPTPRAIWLGERVTVERTGSATRLVLTGRAHVERVPLRRPVAEWAAAWL
                     SAAAPGKRGGAGYPTIGELSTSYPGPGSFKEWTQGRAWRSIRSAGLLVI"
BASE COUNT          756 a         1498 c         1426 g          751 t
ORIGIN      
        1 aatccaggag cgcgccaagg cagtgagtga tttggtcgcg ttgtttcgcc gtctttaggg
       61 cccttcagga aacttctgtc ctagagcctg ttcaggaatg cgtcagatgc aaggcgccgc
      121 gagcaccgga ccggaacgta ctgagtcgta cgtgaggacc ggaagcgcag cggcaccgcc
      181 gcagatgacc gttcatggac aggctcctag ccgcgggccc ggagtttcgc atcgataaac
      241 tcgcggtggg aaacatgaag caggtccgcg atccgccgga tgaggtgctc ctcgtggcgg
      301 tccttgtacg catccgcgaa cgccacttgc cagagcaatt ccacgatgtg gatcttctgc
      361 tccatgggga aatgcttgtc gatcagctgg gtgaactggt gcgtggacgt ggcggtctgg
      421 gcctcttcct cggccaggcg aagcagcacc tcggtgtcgt gctcagagag gccgaacgtc
      481 ttttgcaccg cctcgccgat gacccggcgt tcttcgtctt tgatctcgtg gtcagcccgg
      541 gagacctcca ccatcaacgc ggccgtggcc aactgcagcg cgcggtccgt gctcgcgggt
      601 tcgccggatg tggagcgaat ccgtgtgtcg aagaattcct gaatcgtacg caacatgcca
      661 tctacctcct cgtcgtgcaa gtcctcgcgg ccccggcagt ataggagctt cctccgggtt
      721 cttcaagccg tgtgcatgtg gcgtactgtg tcattgacac accggctcgg gctcgcgtac
      781 cgtacggttt gtgacgattg atgagaccat ggcgccaatg ccagcgccgt cctcgtcgtt
      841 ctggctccgg ctggcccgac cgatcatcgg gctgtcgccc atggacgggg tcacggacgc
      901 ggcgtttcgg ctgattgcgg cgcggcacgg acggcccgat gtgatgatga ccgagtttac
      961 acacgtggaa ggcatctgcc gcgccatccc ggccgtgctg cggcatctgg actacgatcc
     1021 ggctgaacgg ccggtggtgg cccaggtctt cggctcggag ccagcggcct tttacaaagc
     1081 cgcgcaggtg gtggcggaac tgggattcga cggcctcgac atcaatatgg gctgcccgga
     1141 caaggcggtc gcggcacatg ggtgcggagc cgcgctcatc cagacgcccg agcgggccca
     1201 ggcgattatt cgcgcggccc aagccggggt gcgtgattgg gccgcggggc gcgaccttgc
     1261 gtcggtgggc ctgccggatg agacggtgga agccgttcgt cgtatggcca tgggcgccgg
     1321 cgcaacggac cgtgtgcgtc ggctcatccc cgtgtccgtc aagacccgtt taggatacga
     1381 ccgcgtggtg atcgattcct ggatcaacgt gctcctggag accgagccgg ccgcggtcac
     1441 gatccacggc cggacacttg aacagcgcta ccgcggcgag gcggattggg acgccatcgc
     1501 ccgagcggcc gaactcggcc gacataccgg caccctcgta atcgggaacg gcgacctgcg
     1561 gtccccggcc gacgtgaccc ggcgcgtcct gcagaccggc gtaaacggcg tattgatcgg
     1621 acgcgccgcc ttgggaaacc cgtggatctt ccgcgcgact gacgagatcc gcgcagcgtg
     1681 cctggattcc gagatccatg cacccgtcgc gccggtggtg gacgcgcgcg aacgctgggc
     1741 cgtggcgcgc gagcacgccg cggcgttcga ccgcgtgcgt ggacgcctcg cgttcgcggt
     1801 gatgcgcaaa cacctgggct ggtacgtgcg cgggaacgaa gaccggccgg gccttcgcgc
     1861 gcgcctgatg caggttcacg acctcgcgga gttggacgcc gtgctggccg actccgcgcc
     1921 gtgggcgttc accaccgctg aagaccctgc tcccatcgca tcgccgacgt gcgtgtgatc
     1981 ctggcgtcta cctcgccccg gcggcgggag ctgctcgctc gcctcggcgt gccgtttgaa
     2041 gtcgcatccc cgaagtttga agaagtgatc gatcccctcg cgtctccgga aacgcaggcc
     2101 gagcagttcg cgctcggcaa agcgcggtcg attgttgcga cggacgcggt cgtgctgggc
     2161 agcgacactc tggtcgtggt ggaccggacc attctcggca agccaaccga cgccgccgat
     2221 gcccggcgga tgctccgtct gctgtgcggc cgctcgcacc gtgtcatcac cgcggtcgcg
     2281 gtcgtggcgc cggggcaacc ggatcacgtc ttcttggacg aagccgtggt ccggatgcgg
     2341 cctgccgacg acaccgaaat cgcggcgtac gtgtccaccg gagagcccat ggacaaggcc
     2401 ggtgcctatg ccgcgcaagg cgccggagca cgattcatcg aatcaatcac cggcgatgaa
     2461 accacggtca tcggcctccc gctccggcgc ctgaccgagg tgttgcgcaa agtcggaatt
     2521 cggattccag ccgaagcacc tgtctagctg cgacgtgtag ccgtcagcga acggccagcg
     2581 cgaatccctc aatgggcatc ccggctgctc aaaaagtccc agatgcaagg ccgcagtgag
     2641 gaggaacccg gagcgtactg ggtcgtacgt gaggagttcc gacgagcgag aacgcagcag
     2701 atggactttt tcagcagccg gctacgaagc ttgttgctgg gcgtcgaagt accgcgtcac
     2761 gcccgctggg gtggccgtga accagtaccg ttgttcgtgc ggcgtttcgg gactcacgac
     2821 gtgctcgcgt tccaccaccg ccaagagttc gtcgatcccg tcgcttaccg gggcgaatgg
     2881 cgtggcggga tcggaagatt tcacgtacgc ggcgatcaag tgttcggtga gtaatcgttc
     2941 gaggacatcg aggccccggc gaaccagcac cgcccggttg gagagccccg gggtcaatcg
     3001 ctgaaccgcc cacaacagtt cgctcgaccc gcaataatct tccacacagt gacccaacac
     3061 cccacgctcc acatgatcca acaccggtga atccatcgtc gctcctcaga tcaccaacaa
     3121 cccggcgctg cggatcgacc gccacgcccg tccctgcgtc cactccttga acgaccccgg
     3181 ccccggatac gaggtcgaca gctccccgat cgtcggatac cccgcgccgc cccgttttcc
     3241 tggcgcggcg gccgaaagcc acgcggccgc ccactcggca acgggccgac gaagcggtac
     3301 gcgttcaacg tgggcccggc cggtcaatac cagccgggtg gcgctccccg ttcgctccac
     3361 cgtcactcgc tcacccagcc agatcgcgcg cggggtgggg tctagtatag ccggttcagc
     3421 gatacgctgc gcgcgctgca cccactcggc cgacacacgg gggcggggca cccggatctc
     3481 gaaccacgtc cgcacatcgc ggtccaagcc cagtccgtgc ataaaatgtt ggacggctcg
     3541 ggccaatccc gcacccaagg cctccaggtt tccggagcgg ggatcttggt ggaccagatc
     3601 gttgcgcgcg aacgcgcgcg ccggcagcgg ttcgatcgaa atgccgaagg ccgacggccg
     3661 gcgccccacc gggctgtgcc gtgtggcggt gaaccggtgc cagtacgcgg actggatcaa
     3721 atccgcgtcg aacaactggc gcacccgctc cagcgcgtcg attgtttcct gcggcgtttc
     3781 ggtcgggaac ccgtacatca ggtacgcgtg gaccatgatg cccgcgcgag taaacgcccg
     3841 cgccgtccgc gccgcctgcg ccacggtcac ccctttgtcc atcaagtcca gcaggcgatc
     3901 ggaggccgtc tccagccctc cgctcaccgc aatgcacccc gacgcggcga gcaaccggca
     3961 cacgtccggc gtaaacgccg tctcgaaccg gatattgccc caccacgaca cgacccgacc
     4021 gcgctccaac aacgcgagcg cgaggtccac caacctggcg ggaggcgctg cttcgtcgac
     4081 caagtgaaag ccggtttccc cggtttcggc gatgagcgcg tcgatacggt caaccagcac
     4141 ggcggccgaa gccggatcgt accgcttgat atagtcgaga tccacgtcgc agaacgtgca
     4201 ccgtttccag tagcagccgt gcgccacggt caatttgttc caccgtccgt cggaccagag
     4261 ccggtgcatg gggttcggca cctccacgat cgacagatat cgcgaccggt cgagccccgc
     4321 gtacgtgggc gtgccgacct cctcgaacgc cacgtccggt tcgcccgccc cgttcacgta
     4381 ggctacccgt cccttcaccc gcgtaaacgt ccggcaaagc cggtccgctt c
//
LOCUS       DBNGTT010000189         4176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_932762, whole genome shotgun sequence.
ACCESSION   DBNGTT010000189 DBNGTT010000000
VERSION     DBNGTT010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_932762"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /gene="rodA"
                     /locus_tag="AC16BC_04305"
     CDS             <1..1032
                     /gene="rodA"
                     /locus_tag="AC16BC_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR3240872.1"
                     /translation="IYSASVHQSGQPSPLYLRQVAWVGLGLVVFLATALIDYHTMALY
                     AYVGYAAIVVLLVVVLAVGRVGMGAQRWISLGFFDVQPSELAKLVLILVLARYCADKG
                     VKGGLGPTQLVRPLLLAFVPIALILKQPDLGTGLTLLAIAMAMILLVGIRYQSIVFLS
                     LFGLMMVPFAWHLFWNSLKEYQRNRLLTFINPSSDPLGTGYHVNQSKIAIGSGGLWGK
                     GFFGSTQSQLDFLPEGHTDFIFAVFAEEWGFLGVVCLVALYGFLLYWIMETAYKAKDR
                     LGAFLVIGVAAMVVFSLVVNIGMTLGIMPVVGVPLLLMSYGGSSMLTVFAALGLVLNV
                     RLRRFMLFY"
     gene            1092..2681
                     /locus_tag="AC16BC_04310"
     CDS             1092..2681
                     /locus_tag="AC16BC_04310"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR3240873.1"
                     /translation="MGIEIVIDPSREETRVAVLEQQVLTELFIDRKRDRGMVGNVYKG
                     RVVKVLPGMQAAFVDIGVERAAFLYVADIVTESGGPPPAVDSADSPENGDEAETGSDA
                     AKGNDAGEGDGEDEEATVKASPRPRRVLRSIEDLIREGQEVVVQVTKDPIGTKGCRVT
                     TYLSFPGRYLVFMPTVNHIGISRRIGDDGERARLREMMSRLRQPGAGYIIRTVSEGTS
                     EDEFRMDIEFLGRLWQTIRDESQRKSAPALLHADLDLSLRTVRDLLTNKVDRLTVGGR
                     AEYDRIRQFVSTYLPTLVSKIHLYAQDEPIFDRYGIEIEISKALGRRVWLKSGGYIVI
                     EHTEALTVIDVNTGRYVGKRDLEETILKTNLEAAKEIAYQLRLRNIGGIIIIDFIDME
                     KEKNRERVFSALRDALATDRARSNILRISELGLVEMSRERVREDLLRILCDSCLYCER
                     RGYVRSATTVCYEIFREIRRAGSARDAGSILVTVHPNVANLLFDEERDGVEALEREFN
                     KRITIKADPHLHQEQYDLVLV"
     gene            2681..3808
                     /gene="dprA"
                     /locus_tag="AC16BC_04315"
     CDS             2681..3808
                     /gene="dprA"
                     /locus_tag="AC16BC_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR3240874.1"
                     /translation="MNADSTGRDLRRGALALTRVPGVGAVTFRALVAALGDAEAVFRA
                     SPSALKDVPGCPRHVADAIRAFDEWAWVDAELDRLASLGGRLLVAGSSEYPVHLGRIH
                     DPPPALYALGDVATEERRVIAVVGSRRATPYGTATASRLAQELAGSGFVVVSGMARGI
                     DAAAHQGAMAGGGRTLAVLGCGVDVAYPPEMRGLKEQVKTHGAVLSEVPLGAPPDAHH
                     FPTRNRIISGMSLGVLVVEATAESGSLITARQALEQGREVFAVPGNVGTPTSAGTNQL
                     IKAGATLVETADDILDHLGGQLGAAPTRSAAVSSIAIELTDDESRVVDLLSWEPSHVD
                     ELTARAGLAPHRLAEVLLGLELKGMARQVPGRCYVRHPADS"
     gene            3874..>4176
                     /locus_tag="AC16BC_04320"
     CDS             3874..>4176
                     /locus_tag="AC16BC_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR3240875.1"
                     /translation="MAKSLVIVESPSKARTLTKYLGKKYTVMASVGHVKDLPKSKFGV
                     DVEHDFAPSYQVIRGKKKVLDDIKAAAKKADVIYLGPDPDREGEAIAWHIAEEFNGA"
BASE COUNT          711 a         1271 c         1428 g          766 t
ORIGIN      
        1 atttacagcg cgagtgtgca tcaatccgga cagccgtccc ccttgtacct gagacaggtg
       61 gcgtgggtcg gcctcgggct ggtcgtcttt ttagcgaccg cgttgatcga ctaccacacc
      121 atggcgctat atgcctacgt cggatacgcc gcgatcgtgg tcctgttggt cgtggtgctg
      181 gccgtcggcc gggtgggaat gggggcgcag cggtggatct ccctcggctt tttcgacgtg
      241 cagccgtccg agctcgccaa actcgtgttg atcctggtgc tcgcgcgcta ttgcgcggac
      301 aagggggtca agggaggcct gggtccgacg cagctggtgc gcccgctcct cctggcgttc
      361 gtgcccatcg cgctcatcct caaacagcct gacctcggga ccggcctgac gttgctggcg
      421 atcgcgatgg cgatgatcct gctggtgggc atccggtatc agtcgatcgt gttcttgtcg
      481 ttgttcggtc tcatgatggt gccgtttgcc tggcatctgt tctggaattc gctcaaggaa
      541 taccagcgca accgcctgct cacgttcatc aaccccagct ccgacccgct gggtacgggt
      601 tatcacgtca accagtcgaa gatcgccatc gggtccggcg ggttgtgggg gaaggggttt
      661 ttcgggagca cgcagagcca gctggatttt ctgcccgagg gtcacaccga cttcatcttc
      721 gccgtgttcg ccgaggaatg gggattcctc ggggtcgtct gcctcgtggc gctctacgga
      781 tttctgttgt attggatcat ggaaacggcg tacaaagcca aggaccggct cggggcgttc
      841 ctggtgatcg gggtcgcggc catggtggtc ttttccttgg tcgtgaacat cggcatgacg
      901 ctcgggatca tgccggtggt cggcgtgccc ctcttgttga tgagttacgg gggctcctcg
      961 atgttgacgg tcttcgcggc cttgggcctg gtgctcaatg tacgcctccg acggtttatg
     1021 ctattttact gagccccgat ggggccgggt gtccgggagt ttgagcgggc ctccgagtga
     1081 gggagttcgg catgggtatt gagatcgtta tcgatccctc gcgcgaagaa acgcgcgtgg
     1141 cggtgcttga acagcaggtg ctgaccgagt tgttcatcga ccgcaagcgg gatcgcggca
     1201 tggtcggcaa cgtgtacaag gggcgcgtgg tcaaagttct ccccgggatg caggcggcgt
     1261 tcgtcgacat cggcgtggag cgcgccgcgt ttctctacgt ggcggatatc gtgaccgagt
     1321 ccggaggacc gccgcccgcg gtggactccg cggattcccc cgagaacggg gacgaggccg
     1381 agaccggaag cgacgccgcc aaaggcaacg acgcgggcga gggggatggc gaggacgagg
     1441 aagcgacggt gaaggcctcg ccgcggccgc gtcgggtgct gcggtcgatc gaagatctga
     1501 tccgggaggg ccaagaggtc gtggtgcaag tcaccaagga cccgatcggc accaagggct
     1561 gccgggttac cacctacctg tcctttcccg gtcgctacct ggtattcatg cccacggtca
     1621 accacatcgg catctcccgt cgcatcggcg acgacgggga gcgggcgcgg ctgcgcgaaa
     1681 tgatgtcccg gctgcggcag ccaggcgccg ggtacattat ccgtacggtc agcgagggga
     1741 cgagcgaaga cgaatttcgc atggacatcg agtttctcgg gcgcctgtgg caaaccatcc
     1801 gcgacgagtc gcagcgcaag tccgcgccgg ccctgctgca cgccgacctc gacctgtcgc
     1861 ttcggaccgt ccgcgatctg ctgaccaaca aggtggatcg gctgaccgtg ggcgggcgcg
     1921 cggagtacga tcggatccgg cagttcgtga gcacgtacct gcccaccttg gtgtcgaaga
     1981 tccatttgta cgcccaagac gaaccgattt tcgaccggta cgggatcgag atcgagatct
     2041 ccaaggcgtt gggacgacgc gtctggttga agtcaggcgg ctacatcgtg atcgagcata
     2101 ccgaggcgct gaccgtgatc gacgtgaaca ccggtcggta cgtgggcaaa cgggacctgg
     2161 aagagacgat cctcaaaacc aatctcgagg ccgccaagga aatcgcctac cagctccgat
     2221 tacgcaacat cggcggcatc attatcatcg acttcatcga catggagaag gagaagaacc
     2281 gcgaacgcgt cttctccgcg ctccgcgacg cgctggcgac cgaccgcgcc aggagcaaca
     2341 tcctgcggat ctcggaattg ggattggtgg aaatgtcccg cgagcgcgtg cgcgaagatt
     2401 tgctgcgcat cctgtgcgac tcgtgtctgt attgcgaacg gcggggctac gtgcggtccg
     2461 ccaccaccgt ctgttatgaa attttccgcg agatccgtcg cgccgggtcc gcccgcgacg
     2521 cgggaagcat cctcgtgacc gttcacccca acgtcgccaa cctgcttttc gacgaggaac
     2581 gggacggggt cgaggccctg gagcgagaat ttaataagcg gatcacgatt aaggccgacc
     2641 cccacctgca ccaagaacag tacgatctcg tcctcgtgta atgaacgccg actccacggg
     2701 tcgcgacctg cgccgggggg cacttgcgtt gacgcgggtg ccgggcgtcg gggcggtgac
     2761 ctttcgggcg ctggtcgccg cgctgggtga cgcggaggcc gtgttccggg cgtcgccatc
     2821 cgccctcaag gacgtgccgg ggtgtcctcg acacgtggcg gacgcgatcc gggcgttcga
     2881 cgagtgggcg tgggtggacg ccgagttgga tcgtctcgcg agcctgggcg gtcggttgct
     2941 ggtcgcgggc tcatccgaat acccggttca cctggggcgc attcacgacc cgcctccggc
     3001 gctctatgcg ctcggcgacg tcgcaaccga agaacgacgg gtgatcgcgg tggtggggtc
     3061 gcgtcgggcc acgccgtacg gcacggcgac cgcctcgcgg ctcgcgcagg aactggcggg
     3121 ctccgggttc gtggtggtga gcggcatggc gcgggggatc gacgccgcgg cgcaccaggg
     3181 cgcgatggcc gggggcggtc gcacgctggc ggtgttgggc tgcggcgtgg acgtggcata
     3241 cccgccggaa atgcgcgggc tcaaggaaca ggtcaaaacc cacggggcgg tgctctcgga
     3301 agtgccgttg ggcgcgccgc ccgacgcgca ccatttcccc acgcgcaatc gcatcatcag
     3361 cggcatgtcg ctcggcgtgc tggtggtcga ggcgaccgcg gaaagcggct cgctcatcac
     3421 cgcgcgtcag gcgctcgaac aggggcgcga ggtgttcgcg gtgcccggca acgtgggcac
     3481 gcccacgagc gccggaacca atcaactgat caaggccggc gccaccctgg tggaaaccgc
     3541 ggacgacatc ctggaccacc tgggcggcca actcggcgcc gcgcccacgc gctccgcggc
     3601 cgtctcgtcc atcgccatcg aattgaccga cgatgaatca cgggtggtgg atctgttgtc
     3661 gtgggaaccc tcgcacgtgg acgaattaac cgcgcgcgcc gggctcgcgc cgcaccggtt
     3721 ggccgaagtg ctgctgggcc ttgaactcaa aggcatggcg cgacaagttc ctggccggtg
     3781 ttacgtccgc caccccgcgg attcgtgacg gtctgacgga atcgggtata atccctccca
     3841 cgagtctcgg ttgggaggat gtcagcgcat cccatggcta aatcactggt catcgttgag
     3901 tcgccgtcca aggcgcgaac gctcaccaag tatctgggca agaagtacac cgtcatggcc
     3961 tcggtcggcc acgtcaagga tttgcccaaa agcaagttcg gcgtggacgt ggagcacgat
     4021 ttcgccccga gctaccaggt gatccgcggc aagaagaagg tcctcgacga catcaaggcc
     4081 gcggccaaga aggccgacgt catctacttg gggcctgatc ccgaccgcga gggcgaggcc
     4141 atcgcgtggc acatcgccga agagttcaac ggcgcc
//
LOCUS       DBNGTT010000190         2836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_38740, whole genome shotgun sequence.
ACCESSION   DBNGTT010000190 DBNGTT010000000
VERSION     DBNGTT010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_38740"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC16BC_04325"
     CDS             complement(<1..704)
                     /locus_tag="AC16BC_04325"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3240876.1"
                     /translation="MAESRPSLLPVDAALKTILSAVRPLGTERVPVRETLGRVLAQDI
                     TARRDVPPWDNSSVDGYAVRAADVASATAERPVALTVIEEIPAGRMPTQTVSDGQASR
                     IMTGAPMPAGADAVVMVEDTALDGDRVMIRAAAPAGDSVRRRGQDVRAGATVLSAGRR
                     VSPAGAGMAASLGYGIVEVGRRPCVGVLATGDELIDVGEPEQADRLYDVNSYSIAAQV
                     AEAGGIAAALGIARDTP"
     gene            complement(697..1233)
                     /locus_tag="AC16BC_04330"
     CDS             complement(697..1233)
                     /locus_tag="AC16BC_04330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3240877.1"
                     /translation="MADLHDVSDPAPLLVEFRDRLPKGAALDLAMGYGRHALYLAAAG
                     WDVHGVERDPDAVSACRREAERRGLSLRIEQADLDAYRIPANAYDLVVVFYFLDRALI
                     PQIRDAIKPGGAIVYETFGIENQRRFGRPRRTDFCFQPGELAAAFAGFRVLASREGLV
                     GGQYVAQLVAIKPEAVGG"
     gene            complement(1446..1631)
                     /locus_tag="AC16BC_04335"
     CDS             complement(1446..1631)
                     /locus_tag="AC16BC_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240878.1"
                     /translation="MTSCRACAASIRGAGELCGGCAAKALSCRVCGHEFRYADILAQL
                     PITQCPTCEGRAIDVSE"
     gene            complement(1628..>2836)
                     /locus_tag="AC16BC_04340"
     CDS             complement(1628..>2836)
                     /locus_tag="AC16BC_04340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3240879.1"
                     /translation="LAPPCIWPVPERADNTISQPQSTRTSSGLVETTGRGLCFGDGLA
                     PQGERVAFALESDRFVLWPDSPREHPVSYEAVRIEPGGFERLQFKLTWAEQDVPWALV
                     LDDTALARRVRANPPPGLRGEVQRLDALFRRGRFLSGIGWTIVGGIALGPVLVLSLLW
                     WQADRIVGLAADRVPVSWERELGESAFKQMTLGATMLTEGPAVDAVAAVGSRLTAQIH
                     SPYTFQWHVIDDRQVNAFAVPGGHVVVFTGLLQEAESAEELAGVLAHETQHVVLRHSV
                     HGLIRSLGWSATLQILFGSSGPLSGVGEAASRLGNLKFNREQETAADVEGVKLLQRAR
                     IDPRGMIAFFEQLSSHENGIALLSTHPMSADRAERLRKEIERAGAWPTEPLPVAWSDV
                     KAALARGGTP"
BASE COUNT          468 a         1067 c          894 g          407 t
ORIGIN      
        1 ggggtatccc gcgcgatgcc cagggccgcg gcgatccccc cggcttccgc gacctgcgcc
       61 gcgatcgaat aactgttcac gtcgtacagg cgatccgcct gttccggctc gcccacgtcg
      121 atcagctcgt cgccggtggc cagcaccccc acgcacggcc tgcgtccgac ctccacgatc
      181 ccgtatccca atgacgcggc catgcccgcg ccggccgggc tcacgcgccg tcccgcggac
      241 aacacggtcg cgcccgcgcg cacgtcctgg ccgcgtcggc gcaccgagtc gccggccggc
      301 gccgcggcgc ggatcatcac gcgatcgccg tcgagcgcgg tgtcctccac catcaccacc
      361 gcgtccgcgc ccgccggcat gggcgcgccg gtcatgatgc gcgaggcctg cccgtccgag
      421 acggtttgtg tcggcatgcg gcccgcggga atttcttcga tcacggtcag cgccacgggg
      481 cgctccgccg tcgcggacgc gacgtcggcc gcccgcaccg cgtaaccgtc cacggacgaa
      541 ttgtcccacg gcggcacgtc ccgccgcgcc gtgatgtcct gagccaggac ccggccgagc
      601 gtttcacgga ccggcacgcg ctccgtcccc agcggccgca ccgcggacag gatcgtcttg
      661 agcgcggcgt ccaccggcag caacgaggga cgcgactcag ccaccgaccg cctccggctt
      721 gatcgccacg agttgcgcca cgtactggcc gccgaccaac ccctcgcggg acgcgagcac
      781 gcgaaacccc gcgaacgcgg cggccaattc tccgggttgg aagcagaagt ctgtccggcg
      841 cgggcggccg aagcgccgct ggttctcgat gccgaacgtt tcgtacacga tcgcgccgcc
      901 gggtttgatc gcgtcccgga tctgggggat caacgcgcga tccagaaaat agaacacgac
      961 cacgagatcg taggcgttcg ccgggatgcg gtaggcgtcg agatccgcct gctcgatccg
     1021 caaggacaac ccgcggcgtt ccgcctcgcg gcggcacgcg gacaccgcgt cggggtcgcg
     1081 ctcgaccccg tgcacgtccc agcccgccgc ggccaaatac agcgcgtgcc ggccgtaccc
     1141 catcgccaaa tcgagcgcgg cgcccttggg cagccggtcg cggaactcca ccaacagcgg
     1201 cgccgggtcc gacacgtcgt gtaaatcagc cactagcaac ctttccgaga agcgcttgtg
     1261 gagcctgttc agggaaggtc agatgcaagg cgaacccgcc tcgcgccaac ggcgcgaaat
     1321 ggaaacggcg tggtccgctc gggtggcgaa gcaagggagg accttgcgcg cggagcgtac
     1381 tgggtcgtac gtgagcacgc aaggccgacc gacaacgccg cagatggcct ttcatgaacg
     1441 ggctcctatt cgctcacgtc gatggcgcgt ccctcgcacg tcggacactg ggtgatgggc
     1501 agttgcgcga gaatgtccgc gtatcgaaat tcatggccgc acacccggca cgacagcgcc
     1561 ttggcggcgc atcctccgca caactctccc gcgccgcgta tcgacgcggc gcacgcgcga
     1621 caactcgtca tggtgtccct ccccgcgcga gcgcggcctt cacgtcggac cacgccaccg
     1681 gcaatggttc ggtcggccac gcgcccgctc gttcgatttc cttgcgcaac cgctccgcgc
     1741 ggtccgcgct catcgggtgc gtggacaaca gcgcgatccc gttttcgtga ctcgacagtt
     1801 gttcgaagaa cgcgatcatg ccgcgcggat cgatcctcgc gcgttgcagc agcttcaccc
     1861 cctccacgtc ggccgcggtc tcctgctcac ggttgaattt caggttgccc agccgcgagg
     1921 ccgcctcgcc cacgccggat aacgggcccg aacttccgaa gagaatctgc agcgtggcgc
     1981 tccagcccag cgaccggatg agtccgtgca cgctgtgacg cagcaccacg tgctgggttt
     2041 cgtgcgcgag caccccggcc agttcctccg cggattccgc ctcttggagc aagcccgtga
     2101 acacgaccac gtgtccgccc ggcaccgcga acgcgttgac ctggcgatcg tcgatcacgt
     2161 gccactgaaa cgtgtaggga gagtggatct gggcggtcag ccgcgagccc accgccgcga
     2221 ccgcgtccac cgcggggccc tcggtcagca tggtcgcccc gagcgtcatt tgcttgaacg
     2281 cggactcgcc gagttcccgc tcccacgaca cgggcacgcg atcagcggcc aaccccacga
     2341 tccgatcggc ctgccaccag agcagcgaca acaccaggac cggccccagc gcgatcccgc
     2401 cgacaattgt ccacccgatc ccgctgagaa accgcccgcg ccgaaacaac gcatcgagtc
     2461 gttggacctc gccccgcagg cccggcggcg gattcgcgcg aacgcgccgc gccagggccg
     2521 tgtcgtccag caccaaggcc cacggcacgt cctgctccgc ccacgtcagt ttgaactgta
     2581 agcgctcgaa cccgcccggt tcgatccgta cggcctcgta ggacacggga tgttcgcgcg
     2641 gcgagtccgg ccacaatacg aagcggtccg actcaagcgc gaacgccacc cgctccccct
     2701 gcggcgccaa gccatcgccg aaacacaacc cgcgcccggt ggtttccact aggcctgaag
     2761 atgttcgcgt cgactgcggc tggctgatgg tgttgtcagc ccgttcagga acgggccaga
     2821 tgcaaggcgg agcgag
//
LOCUS       DBNGTT010000191         2904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2721695, whole genome shotgun sequence.
ACCESSION   DBNGTT010000191 DBNGTT010000000
VERSION     DBNGTT010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2721695"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1480
                     /locus_tag="AC16BC_04345"
     CDS             221..1480
                     /locus_tag="AC16BC_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HIR3240880.1"
                     /translation="MIRPPRVLLRFARLFPPCAVLAAAACAAPHGHAPAAPAAQPAPH
                     VESAESPYRALNGLPEGTILHVPSGVSLTPDQLFDLLGAARVVYVGESHDNLSHHRIQ
                     LDIIRALAERYPGQVAVGMEMFQRPAQPALDRWTRGELSAKEFESLWYENWTEDYAYY
                     RAILEFVRDRRIPLIALNAAERTARALSGRQPDALSPEERAGLPEIDTQDPYHRRQME
                     AVFGGHAKGSGFDPFYRTMLLWDETMAQTVADFVSGPAGRDRRLVVLAGGGHIAYGFG
                     IPRRAFRRAPVPYVTVIPQTEVEMAPADRPDLVMQVESFSVPLPIADIVWAVGYEDLP
                     SPSVRLGVRIEAAEGGVTITSVDSGSAAERAGLREDDVIVSFDGETVRRPADVVRLVR
                     GHGPGDHASLTVTRGAETMTLDVSWPK"
     gene            1477..2340
                     /locus_tag="AC16BC_04350"
     CDS             1477..2340
                     /locus_tag="AC16BC_04350"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HIR3240881.1"
                     /translation="MTVRPTSPERTDAAPGAPFYIFLISDATGETAARIADAALSQFE
                     RRDVVFIRRPHVRSEEHIRQVVREARSVHGTILHTLVSSTTRARLVEATKLYGVPALD
                     LIGPLLAHLESFFGSRPRAEPGLLHRMDEEYVRRIEAVQFTVQHDDGQSVQTLDQSDI
                     VLVGVSRTGKTPLSMYMAQYGWKVANVPIVFGVPPPKELLAMEPSKVVGLTVSPDKLL
                     TVRAARARKFGHLDMAYATNDGITQELEYCRSLFTANRGWRVLDVTTRAVEEIAADIM
                     SALPSPGPRPG"
     gene            2626..>2904
                     /locus_tag="AC16BC_04355"
     CDS             2626..>2904
                     /locus_tag="AC16BC_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3240882.1"
                     /translation="MPQPEIESVLNERRVFKPKAAFSRAAHVKSLKEYDKLAKLAQKN
                     PEKFWSDLAKELYWFKRWKKVLEWKPPFAKWFVGGQTNVSVNCLDRHLH"
BASE COUNT          486 a         1035 c          920 g          463 t
ORIGIN      
        1 cccgccgccc ccaagaccga ccatcccgag cccccaggcg cggatgccga cccgcttcag
       61 cgatggcggg aggcacgcga gatggaagag gagctcaacg agatgttcga ggaagatcgc
      121 gtcacccacc ggcacgggga cgccgagccg gagtcggatt gaccgcggac gccgtccacc
      181 ggcattcccc gcccgccgag gcattcgcac gacgctgatc gtgattcgtc cgccgcgcgt
      241 cttgctccgg ttcgccagac tgtttccccc gtgcgcggtc cttgccgcgg cggcgtgtgc
      301 cgcgccgcac ggacacgccc cggccgctcc cgcggcccag cccgcgccgc acgtcgagtc
      361 ggccgaatcc ccctatcgcg ccctgaacgg actgcccgag ggcacgatcc tgcacgtccc
      421 gtccggcgtt tcgctgaccc cggaccaact gtttgacctg ctgggagccg cgcgcgtggt
      481 gtacgtgggc gaatcccacg acaacctcag ccaccatcgg attcaactcg atatcatccg
      541 cgcgctcgcc gaacggtatc ccgggcaggt cgcggtcggc atggaaatgt tccaacgtcc
      601 ggcgcagccc gcgctcgatc ggtggacccg cggggaactg tccgccaaag agttcgagtc
      661 gctctggtac gagaattgga ccgaagacta cgcgtattac cgcgccatcc tcgagttcgt
      721 ccgtgaccgc cgcattccgc tcatcgcgct caacgcggcg gaacgcaccg cccgcgcgct
      781 ctcgggccgc caacccgacg cgctgtcgcc cgaggaacgc gcggggttgc cggagatcga
      841 cacccaggac ccctatcatc ggcggcaaat ggaagcggtc ttcgggggac acgccaaggg
      901 atcgggcttc gatccgttct accgcaccat gctgctctgg gacgaaacca tggcgcagac
      961 cgtggcggac tttgtctccg gtccggccgg ccgcgacagg cgcctggtcg tgctcgccgg
     1021 gggcggtcat atcgcctacg gattcgggat tccccgccgc gcgtttcgcc gggcgcccgt
     1081 cccctacgtc accgtgattc cgcagaccga ggtggagatg gcgcccgcgg atcgccccga
     1141 cctggtgatg caggtggagt cgttctccgt gccgcttccg atcgcggaca tcgtctgggc
     1201 cgtaggctac gaagatctcc cctcgcccag cgtccgtctc ggcgtgcgca tcgaggccgc
     1261 cgagggcggg gtgacgatca cgagcgtcga ttccgggtcg gccgccgagc gcgcgggcct
     1321 gcgcgaagac gacgtgatcg tgtcgttcga cggagaaacc gtgcggcgcc cggccgacgt
     1381 tgtgcgtctg gtgcgcggcc acgggcccgg ggaccacgcg tccctgaccg tcacgcgcgg
     1441 cgccgagacc atgaccctcg acgtgtcgtg gccgaaatga ccgtccggcc gacctcgccc
     1501 gagcggaccg acgccgcgcc cggcgcgccg ttctacatct ttctgatctc cgacgccacc
     1561 ggggagaccg cggcccggat cgcggacgcg gcgttgtcgc aattcgagcg acgcgacgtc
     1621 gtctttatcc gccgccccca cgtgcgcagc gaggagcaca tccggcaggt cgtgcgcgag
     1681 gcccgctcgg tccatggcac gatcctgcac accctcgtgt cgtcgaccac gcgcgcgcgc
     1741 ctggtcgagg cgaccaaact ctacggcgtg cccgcgttgg acctgatcgg tccgctgctc
     1801 gcgcacctgg agtcgttttt cggatcgcgc cccagggccg aacccgggct gttgcaccgg
     1861 atggacgagg agtacgtcag gcgcatcgaa gccgtgcagt ttacggtcca gcacgacgac
     1921 ggccagagtg tgcaaaccct cgaccagagc gacatcgtgt tggtcggcgt gtcgcggacc
     1981 ggcaagactc cgctctccat gtatatggcg cagtacgggt ggaaagtggc gaatgtcccg
     2041 atcgtgttcg gcgttccgcc ccccaaggag ctcctggcca tggagccctc caaggtcgtg
     2101 ggcctgaccg tgtcgccgga caagctgctc accgttcgtg ccgcccgcgc cagaaaattc
     2161 gggcacctgg acatggccta cgccaccaac gacggcatca cacaagaact ggaatactgc
     2221 cgctcattgt tcacggccaa ccgcggctgg cgcgtcctgg acgtgaccac gcgcgcggtc
     2281 gaggaaatcg ccgcggacat catgagcgcg ctgccctccc ccggcccccg gccggggtag
     2341 cgcctgtcca tgaaaggcca tctgcggcgt tgtcggtcgg ccttgcgtgc tcacgtacga
     2401 cccagtacgc tccgcgcgca aggtcctccc tccgcctggc atctggcctt ccctgaacag
     2461 gctggagtcg caaaccgcaa atccacttct gagacggtat tcccctaatt gacaagtcgg
     2521 gcggcccttc cctacaatgc tcgactcaac gctccggccg catgattcgc gcgactcggc
     2581 gcgaattgcc tcgcagacgc cactgacctc gaaggagact ccgcgatgcc ccaacccgag
     2641 atcgagtccg ttttaaacga acgccgcgtc tttaaaccca aggcggcgtt cagccgggcc
     2701 gcccacgtca aaagcctcaa ggagtacgac aaactcgcca aactggcgca gaagaacccg
     2761 gaaaaattct ggagcgacct ggccaaggag ctctattggt tcaaacggtg gaaaaaggtg
     2821 ctggagtgga agccgccgtt cgccaagtgg ttcgtgggcg gccagaccaa cgtgtcggtc
     2881 aattgccttg accggcacct gcac
//
LOCUS       DBNGTT010000192         2458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2338254, whole genome shotgun sequence.
ACCESSION   DBNGTT010000192 DBNGTT010000000
VERSION     DBNGTT010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2338254"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC16BC_04360"
     CDS             complement(<1..706)
                     /locus_tag="AC16BC_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3240883.1"
                     /translation="MNIDELLKAAVAKRGSDLHLKVGCAPTVRVDGHLEPLDQSIRLT
                     QEDAVAVAFSIMNNTQKQRFKEKSEIDLAYSAPGLGRFRVNCYQQRGTVNMVFRSVPT
                     KILTFQDLHLPSVIEKLATETRGLILVTGTTGSGKSTTLAAIIDHINRTRTENIITIE
                     DPIEFLHRDRKCLVSQREIGADTESFSSALRSALRQDPDVILVGEMRDFETISTALVA
                     AETGHLVMSTLHTVDAT"
     gene            complement(709..1758)
                     /gene="recA"
                     /locus_tag="AC16BC_04365"
     CDS             complement(709..1758)
                     /gene="recA"
                     /locus_tag="AC16BC_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR3240884.1"
                     /translation="MIAKDKEQQGNRMKALDLALAQLEKQYGKGTIMRLGADGAVAET
                     AVIPSGSLGLDIALGVGGLPRGRIVEIFGPESSGKTTLSLQAVAAAQADGGVAAFIDA
                     EHALDMAYAKRLGVNTDDLLVSQPDTGEQALEITETLVRSGALDVVVVDSVAALVPRA
                     ELEGEMGDSHMGLQARLMSQAMRKLTAAISKSHTTVIFINQIRMKIGVMFGNPETTTG
                     GNALKFYASVRLDIRRTDALKDGQDVVGSRVRVKVVKNKMAPPFRQADFDIMFNEGIS
                     RLGEVIDLGVEKGIIEKSGAWYAYKGDRIGQGRENAKHFLREQAPVAKEIESRIREAY
                     GLTPKEKAVHKGADA"
     gene            complement(1954..>2458)
                     /gene="thpR"
                     /locus_tag="AC16BC_04370"
     CDS             complement(1954..>2458)
                     /gene="thpR"
                     /locus_tag="AC16BC_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR3240885.1"
                     /translation="AGGIRWVQPDGAHVTLAFLGDVPAARVPALAARLRDAGRECAPC
                     RVSADAIGVFPHPGRAAVLWLGIKDPAEGLRRLQGRVQVALGAEGFAAEDRPFHPHVT
                     LGRWRTPPSRDRVEALLATPLPLALSGGAGEWVVTEIRLMASQLAPEGPVYTVMAALP
                     LTGSAPG"
BASE COUNT          383 a          883 c          726 g          466 t
ORIGIN      
        1 cggtcgcgtc cacggtgtgc agggtgctca tgaccagatg cccggtttcc gccgccacca
       61 atgcggtgga aatcgtctcg aagtcgcgca tctcgccgac cagaatcacg tcggggtcct
      121 ggcgcaaggc cgagcgcagc gcgctgctga acgactccgt gtccgcgccg atttcgcgct
      181 ggctgaccag acacttgcgg tcgcgatgga gaaactcgat cggatcttca atggtgatga
      241 tattttcggt gcgggtgcgg ttgatgtggt cgatgatcgc ggccagcgtg gtggatttgc
      301 cgctcccggt ggtcccggtc accagaatca acccgcgggt ttccgtcgcc agcttctcga
      361 tcaccgacgg gaggtggagg tcctggaacg tgaggatctt ggtcgggacc gaccggaaca
      421 ccatgttcac ggtcccgcgc tgctgataac aattgacgcg gaatcggccg agccccggcg
      481 cgctgtacgc caggtcgatt tcggactttt ccttgaaccg ctgcttctgc gtgttgttca
      541 tgatcgagaa cgccaccgcc accgcgtcct cttgcgtgag ccgaatcgac tggtcgagcg
      601 gctcgaggtg tccgtcaacg cggaccgtcg gcgcgcaccc cactttcaga tggagatcgg
      661 accctcgctt ggctaccgcc gctttcagca gttcgtcgat gttcaaactc acgcgtcagc
      721 ccccttgtgc accgccttct ccttcggagt cagcccgtac gcctctcgga tgcgggattc
      781 gatttccttc gcgaccgggg cttgctcccg gagaaagtgc ttggcgtttt cccgcccctg
      841 cccgatccgg tcgcccttgt aagcatacca ggcgccgctc ttctcgatga tccccttctc
      901 gacgcccagg tcgattactt cgcccaaccg cgaaatgcct tcgttgaaca tgatatcaaa
      961 atccgcctgg cggaacggcg gcgccatctt gttcttgacc accttcacgc gcacgcggct
     1021 gcccaccacg tcctgcccgt ccttgagcgc gtccgtgcgg cggatatcca ggcgcaccga
     1081 cgcgtagaac ttgagcgcgt tgcccccggt ggtggtctcg gggttgccga acatcacgcc
     1141 gatcttcatc cggatctgat tgatgaagat cacggtggtg tgcgacttgc tgatggcggc
     1201 ggtcagtttg cgcatggcct gggacatcag gcgggcctgg aggcccatgt gcgagtcccc
     1261 catttcgccc tccaattccg ctcgtggcac cagcgcggcc accgaatcca ccaccaccac
     1321 gtccaatgcg ccgctccgca ccagcgtttc ggtaatctcc agcgcctgct cgccggtgtc
     1381 cggttgggaa accaggagat catcggtatt gactcccagt cttttggcgt aggccatgtc
     1441 caacgcgtgt tccgcgtcga tgaacgccgc gaccccgccg tcggcctgcg ccgcggcgac
     1501 cgcttgaagc gaaagcgtgg tctttcccga ggactccggg ccgaagatct caacgatccg
     1561 cccgcgcggc agcccgccga cgcccaacgc gatgtccaac cccaacgacc ccgaggggat
     1621 gaccgcggtt tccgccaccg cgccgtccgc gcccaaccgc atgatcgtgc ccttgccgta
     1681 ttgcttttcc aactgcgcga gcgcgagatc cagcgccttc atccggttgc cttgctgttc
     1741 tttgtctttt gcgatcatcc tcgactcctc cttaagtagg agcgcgttga tgaacggcca
     1801 tctgcggcgt tgtcgctgcg cgtccgctcc tcacgtacga cccagtacgc tgcggtgcgg
     1861 gcgctcgctc cgccttgcat ctggcgcgtt cctgaacgcg ctcaccaaac ccactgctac
     1921 ggcatcctac tcactagaat gacgccctca tgcttaacct ggcgcagacc ctgtcagcgg
     1981 cagcgccgcc atgaccgtgt agaccggccc ctccggggcc agttggctcg ccatcaaccg
     2041 gatctcggtc accacccact ccccggcgcc gccgctcagc gccaggggca atggcgtggc
     2101 gagcagcgcc tcgacgcggt cacgagaggg cggggtgcgc caccgcccca acgtcacgtg
     2161 gggatggaac ggccggtcct cggccgcgaa tccttccgcc cccagcgcga cttgcacgcg
     2221 cccctgcagc cgccgcaatc cctcggcggg gtccttgatt cccagccaca acaccgcggc
     2281 gcgacccgga tgcggaaaca cgccgatcgc gtccgcgctc acccgacacg gcgcgcactc
     2341 ccgccccgcg tcccgcaacc tggccgcgag cgccggcacg cgcgcggcgg gcacatctcc
     2401 caggaacgcc agggtgacgt gcgcgccgtc cggttgcacc catcggatgc ccccggcc
//
LOCUS       DBNGTT010000193         9928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_166827, whole genome shotgun sequence.
ACCESSION   DBNGTT010000193 DBNGTT010000000
VERSION     DBNGTT010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_166827"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="AC16BC_04375"
     CDS             complement(<1..855)
                     /locus_tag="AC16BC_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3240886.1"
                     /translation="MLDQHLLSIILFAPFVGAAALLFVDGKNLPLVRGIAAGSSGISF
                     AASIYLLYAFNYTPGSAQFQFLEQYEWSKDIGISFFLGVDGMSVPLVLASSLLLFAGV
                     FVSWHIEDRAREFYIFLLILASATIGVYVSLDLFFLYFFYEMSVVPMYPLLGVWGSHT
                     KGYLEMKDTAKRDSVGFIFNFASNSKEYAAMKLTLFLSAGAVVALLGIFLTYVGTGLN
                     TFNLIELQGHEDKFSPVMRSAIWLMLFFGFASIAPLWPMHSWSPVGHAAAPAAVSMLH
                     AGVLMKLGH"
     gene            complement(855..2474)
                     /locus_tag="AC16BC_04380"
     CDS             complement(855..2474)
                     /locus_tag="AC16BC_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3240887.1"
                     /translation="MEQMFAMDQLGVPILTIVTFLPLLGALAVWVLRDDAMAWKLGFA
                     IAGADFLVSIPLFTAFIPDSAALQFVEQYPWISTIGVSYHVGVDGISMLFVVLTTLLG
                     LLIVGYSYGVITERVRGFLMALFALQTTMIGVFVSLDLVLFFVFWELMLIPSYFMIRL
                     WGTEGREYAAVKYVVYTLLGSVLMLVGIVILYLQHWRFASFHGTGSALSFDMLDLLRT
                     RAFMSPEVQMAVFVFMFFGFAFKTPIIPFHTWMPNALYSGPIGMSVILAGVKLGTFGF
                     VRYSIPLAPDAARAALPVMMWLGLIGILYGAYIAIMQADFRKLLAYSSISHLGYVAIG
                     IFALTYQGIQGGLIQMINLGISTAGLFFFAGFLWTRRQTLTISEFGGLSKTMPLLATF
                     FLIMIFSSIGLPGTNGFIGEFLILLGAFKAHWLYGAVGVLGVILGAAYMLWFYERAIF
                     GPVTKPINERLPDLTGRELAITLPLVALVFWIGVYPSPFLHRINGSVEALHARLQPPS
                     RTPIPVGASLNPHETPSAEVPAAQYEGEGTP"
     gene            complement(2474..4468)
                     /gene="nuoL"
                     /locus_tag="AC16BC_04385"
                     /pseudo
     CDS             complement(2474..4468)
                     /gene="nuoL"
                     /locus_tag="AC16BC_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
     gene            complement(4688..4993)
                     /gene="nuoK"
                     /locus_tag="AC16BC_04390"
     CDS             complement(4688..4993)
                     /gene="nuoK"
                     /locus_tag="AC16BC_04390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3240888.1"
                     /translation="MEITLTHYLVVSTIIFSLGLLGVLIRRNFIIVLMAVEMMLNAAN
                     LNLVAFSHHLHSLTGQMVALFTIAIAAGEAAVGLAIIVVIFRGKIATNVDQINLLKW"
     gene            complement(4993..5622)
                     /locus_tag="AC16BC_04395"
     CDS             complement(4993..5622)
                     /locus_tag="AC16BC_04395"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR3240889.1"
                     /translation="MSALAFYYLAAVTIVSGVLTITLRSPVHCGLALVAMLVHMAGFF
                     VLLNAEFIAAVQVIVYAGAILVLYLFVIMLLNLKTDERVFHKRYIGMGLITIAIIAQA
                     AVLMLRSPYAGLTGEATPEVVLGVGHTKAIGIVMFRDYLLPFELIGIYLLAAIVGAIV
                     LAKTPAMSGQSQEPSAPPANGSGNGEGPSGPALEEPVETVGAHHGGHGH"
     gene            complement(5628..6179)
                     /gene="nuoI"
                     /locus_tag="AC16BC_04400"
     CDS             complement(5628..6179)
                     /gene="nuoI"
                     /locus_tag="AC16BC_04400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR3240890.1"
                     /translation="MTLKELFDKAVLREILDGMKTTFKHMFVKPVTFQYPHEKRAIPD
                     THRGALCLLRYDDGKDRCVGCDLCEAACPSRCIKVVSEEDPVTPLTRIATEFYIDITK
                     CVFCGFCVEACPVNALAMTKMYEFSTMNKRDLMFDKDRLYQIGDQLHAEAKSYLVSHH
                     QEDSDEAARQYRYKFPHAPSRRG"
     gene            complement(6220..8886)
                     /locus_tag="AC16BC_04405"
     CDS             complement(6220..8886)
                     /locus_tag="AC16BC_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3240891.1"
                     /translation="MAELISITTDKANVQLTIDDEVVSVPEGTSIYDAIVGMGKTIPS
                     MCYHYTFNPFGSCGVCLVEVEGKKAPVRSCTSPVAAGMIVRVDTKTMFDARKKAVEKH
                     LQTHPLDCPVCDADGHCELQDMSFNFGIVQIGEVKQKGLPEDRRSTVLDFNMNRCILC
                     AQCINICKEVVMVDALQFFKKGGFTHVVAKGDNALDCEFCGDCLAVCPVGAITNKFSK
                     YLYKPWQLNRTMTACNYCADGCQMRVEVKDDAVVRVTSQLSWKDKWDDHTSTVDGHGG
                     LCVKGRFGFAYVNSPDRLKKPLMRKGGKLVEASWFEATAEIAKRLRAIKSEYGPDAIA
                     GLITARCTNEDLYAFQRFMRQAVGTNQIDSSARYGHAVYVEAMERALGVVQPTLSFRD
                     LTMARSILVVGSDLTETNPILSLRVKDAFSNFHAQLIVAHSMRTNLTKLAPYPYQAPA
                     GSEGAFVQGVTKAIVESGLVPARVTTEQGAAVSVLASALAALSWDALRVATGLSREQF
                     AEAAGVFLSGETRAIVLGEQIVNRPDGYANVLRIADLMLASGMVGQPGSGVVRTCEEN
                     NERGAIATGALADRLPGGSRYDDAAARSRIESAWGTTLPTNGATLTEILDGIRAGRIK
                     ALYAVGENPLGTLPASAGVREALAKLDLLIVQDAFLTEMGEAAHVVLPAALAAEKSGT
                     FLDGQGSLQPVVRALDPPGEARTDWEIFSELGQLLGASFDYTEAEEIRRELLQVAPAA
                     FEMPGATRPAAGLSADVAARYARAAVSSGATNAEYPVELSLYQVLYHSGKMSTRDKGL
                     LEINAKKLLQISLKDAEALGLADNGRVKVSSPLGSVEVGVETNLDLPAGSCRFPEHFN
                     DPAVKDVLPMLVDPVTKSPVFKAGRVKFVKVR"
     gene            complement(8981..>9928)
                     /locus_tag="AC16BC_04410"
     CDS             complement(8981..>9928)
                     /locus_tag="AC16BC_04410"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR3240892.1"
                     /translation="FAYVRAVEKLMGITVTPRAEYLRTFVAEVQRIIGHLFWLGTQAL
                     DIGAMTVFFYTFREREVLLDLFDKLCGARLTTSWYRIGGVERDFTQDIIDGFNAFIKD
                     FPAKVEEYNVLLEKNRIWIARTKGIAVISAEDALSFGLSGPTLRGSGVEYDLRKAEPY
                     GAYGDVEWNVPIGVNGDTYDRYWIRIEELKESSKIIRQCLDKLPDGPIMADVPQVVAP
                     TKDRVFTNLESMIMQFKFFSVGFPTPKGEIYCGTEAHKGELGFYIVSNGEGKPYRLKI
                     RAPSFIHMGAFDQMARGYMIADVITIFGTYDIVMGECDR"
BASE COUNT         2043 a         3285 c         2974 g         1626 t
ORIGIN      
        1 gtgccccagc ttcatcaaca cgcccgcgtg cagcatggac accgcggcgg gcgcggccgc
       61 gtggcccacc ggcgaccacg agtgcatggg ccagagcggg gcgatcgacg cgaacccgaa
      121 gaacagcatc aaccagatcg cgctgcgcat cacgggcgaa aacttgtcct cgtgaccttg
      181 cagctcgatc aggttgaacg tgttcagccc ggtgcccacg tacgtgagga agatgcccag
      241 caacgcgacc acggccccgg ccgacaaaaa caaggtcagc ttcatggccg cgtactcttt
      301 gctgttggag gcgaagttga aaatgaagcc cacggagtcg cgtttggccg tgtccttcat
      361 ctccaaatag cccttggtgt ggctgcccca cacgcccaag agcgggtaca tcgggaccac
      421 ggacatttcg tagaagaagt acaggaagaa caaatcgagc gagacatata cgccgatggt
      481 ggcggacgcg agaatcaaca ggaagatgta gaactcgcgc gcacgatcct cgatgtgcca
      541 cgacacgaac actccggcga acaacaacaa cgacgaggcc agcaccaagg gcacggacat
      601 accgtccacg cccaggaaga acgagatccc gatgtccttc gaccactcgt attgttcgag
      661 gaactgaaac tgcgcggagc ccggcgtgta attgaacgca tacaacaggt agatcgacgc
      721 ggcgaacgag attccgctcg aacccgcggc aatgcctcgg acgagcggga gattcttgcc
      781 gtccacgaac agcagcgccg ccgcgcccac gaacggcgcg aacaagatga tcgagagcag
      841 atgctggtcc agcatcaggg cgttccctct ccctcgtatt gcgccgcggg aacctccgcg
      901 gacggcgtct cgtgcgggtt caggctcgcg ccgaccggga tcggcgttcg cgacgggggc
      961 tgcagccgcg cgtggagcgc ctccaccgac ccgttgatcc ggtgcaggaa cggcgacgga
     1021 tacaccccga tccagaacac cagggccacc agcggcagcg tgatggccaa ttcacgaccg
     1081 gtcagatccg gcagccgttc gttgatcggc ttggtcacgg gcccgaagat cgcgcgttcg
     1141 tagaaccaca acatgtacgc cgcgcccagg atcacgccca gcaccccgac cgcgccgtaa
     1201 agccagtgcg ccttgaacgc gcccagcagg atcaggaact cgccgatgaa cccgttggtg
     1261 cccggcagcc cgatcgacga aaagatcatg atcaggaaaa acgtggccag caagggcatt
     1321 gtcttggaca atccgccgaa ctccgaaatc gtgagggtct ggcgccgggt ccagagaaag
     1381 cccgcgaaaa agaacagccc ggcggtgctg atacccagat tgatcatctg gatcagcccg
     1441 ccctgaatcc cctggtaggt gagcgcgaag atcccgatcg ccacgtagcc caggtggctg
     1501 atcgacgaat acgccaggag tttgcgaaaa tcagcctgca tgatcgcgat gtacgcgccg
     1561 tagagaatgc cgatcagccc cagccacatc atcaccggca aggccgcgcg ggccgcgtcc
     1621 ggggccaacg ggatcgagta gcgcacgaac ccgaacgtgc cgagcttcac cccggccagg
     1681 atcacgctca tgccgatggg accggaatag agcgcgttgg gcatccaggt gtggaacgga
     1741 atgatcgggg tcttgaacgc gaacccgaag aacatgaaca cgaagaccgc catctggacc
     1801 tcgggcgaca tgaacgcccg ggtgcggagc agatccagca tgtcgaacga cagcgcagac
     1861 cccgtgccgt gaaacgacgc aaagcgccag tgctgcaaat acaggatcac gatgcccacc
     1921 agcatcaaca cgctgccgag caacgtgtac accacgtact tgaccgccgc gtactcgcgg
     1981 ccctcggtgc cccacagccg gatcatgaaa tacgacggga tcaacatcag ttcccagaac
     2041 acgaagaaca gcaccaggtc cagggacacg aacaccccga tcatggtggt ttgcagcgcg
     2101 aacaaggcca tgagaaagcc gcggacccgc tccgtgatca cgccgtacga gtagcccacg
     2161 atcaacagcc ccagcaacgt ggtcaggacc acgaacaaca tgctgatgcc atccaccccc
     2221 acgtggtagc tgacgccgat ggtggagatc cacgggtact gttccacgaa ctggagcgcc
     2281 gcggaatccg gaataaacgc ggtgaacaac gggatgctga ccaaaaaatc agcccccgcg
     2341 attgcgaacc cgagcttcca cgccatggcg tcgtcgcgga gcacccagac cgccagggcc
     2401 cccagcaacg gcaggaacgt gacgatcgtg aggatcggca cgccgagttg gtccatggcg
     2461 aacatctgct ccatcatcgc cgcgccacca gactggacac gttgccgccg gtccaccacc
     2521 agaacacgta cgccaggatg gccaggccca cgacaatgat gaacgcgtag tggtgcacga
     2581 acccggtctg cagccggcga aacgcgcgcg cgcccaggtg attggagtat ccgatcacgt
     2641 tcaaagacgc gtagatcacg tacttctcga tccaggtcga gagccacgct ccgccctgcg
     2701 tcatcctcgc cacgccccgc accaagccgt cgatcaccgt gttgtcgaac caggcccacg
     2761 cctggctcag gcgtttggac ggctccacga tcagcgcatc gtacagttca tccacccagt
     2821 acttgttgag cagtactcgg tgcgcggccc ggtacttctc gccccacgca gaagccatct
     2881 cgggtttacg cacgtacatg gtgtacgcca ggaaccaccc ggcgatggct accagtaccg
     2941 acgcgcccat caagatcaga tcggtctcgc cgaatccgtg gtgcgcttcc ccgccttcgg
     3001 ccgctgccac cggcgcgagg aagtggtgca gccacccgcc ttccggcggc acgccaagca
     3061 ccaggccgcc caacacggac aacccggcaa ggatcatcaa cggcacggtc atgaccagcg
     3121 gcgattcgtg gacgtggtgc gcgacctcgt ggtccatgcg cgtggtgccg gtaaacgtca
     3181 cgtagaacaa acggaacatg tagaacgagg tcaggaacgc gccgctcatc ccgagcaacc
     3241 acaacatctg atgatcgccc ttgaacgcca tccccagaat ttcatccttg ctccagaacc
     3301 cggccaacgg cggcaacccc gcgatcgcga ccgtgccgat gaggaacgtg atcgcggtga
     3361 tcgggatctc ggccttcagc ccgcccatct tgcggatgtc ctgctccccg cccaacgcgt
     3421 gaatgacgct gccggagccc aggaacagca gggccttgaa gaacgcgtgc gtgaccaggt
     3481 ggaagatcgc cgcggtgtac gcgcccaccc cgcagcccag gaacatgtac ccgagctggc
     3541 tgaccgtgga gtacgcgagc acgcgcttga tgtcggtctg caccaggccg atggtggcgg
     3601 cgaaaatcgc ggtgatcccg ccgatcacgc ccaccaccgt cagcgcggtg ggcgccagat
     3661 catagatcgc gtggttgcgg accaccatat acacgcccgc cgtgaccatg gtggcggcgt
     3721 gaatgagcgc gctgaccggg gtggtgcctt acatcgcgta cggcaaccac gtgtacaacg
     3781 ggatctgcgc ggacttgccg atggcgccga tgaacaggca cagggcgatc gcggtggcca
     3841 tgccctcgga cagctcgtgc gcgcggtcga tcacttccag gtagttgagc gtgccgaact
     3901 cctggatgat cagaaagatc gcgatgagaa atcccgcgtc cccgatccgg ttgaccacga
     3961 acgccttggt gccggccttg gtggcggacg gcttgtcgaa gtagtacgca atcagcaggt
     4021 acgagcacag ccccacgccc tcccacccga tgaacaggac caggtaattg tcccccatca
     4081 ccagcaggag catggagacc atgaacagat tgaggtacgt gaagaagcgg tagtaccccg
     4141 ggtccccgtg catgtagccc atagaataga tgtgaatcag caaccccacg cccgtgacca
     4201 ccatcagcat cacggccgag agcgggtcga tcaggaagcc gaggtgcacg tccaagccgc
     4261 cgccgaaaat ccacgagtac acgggcacgg agatcggcga ctcgccgccc gtgacgcgca
     4321 gcaacagcga gatcgacaat acgaacgcga cggccacgga cccgaccgcg aaatagtgcg
     4381 acgcttcctt gagatacttg cggccgaaca ggctgttgac cagaacggcc aacagcggaa
     4441 gcaagggaat tagaacgaca gaaagcatgt taagaattct ttccgacgtt tccacgtcgg
     4501 agcctgttca ggggcgcgcc agatgccagg cggagcgagc gcccgcaccg cggcgtactg
     4561 ggtcgtacgt gagggcgagt ggcgcgacga ataaggagcg ccttgctacg agcgccgccg
     4621 aggcggtgag gcggccgggg agacgcgcag agacaacgcc gcagatggcc gctcatggac
     4681 aggctcgtca ccatttcaag agattgatct gatccacatt cgtcgcgatc ttgccgcgga
     4741 aaatgaccac gatgatggcc agccccaccg cggcctcgcc cgccgcgatc gcaatcgtga
     4801 acaaggccac catctggccg gtgagcgagt gcaggtgatg ggagaacgcc accagattca
     4861 ggttcgcggc gttgagcatc atctcaaccg ccatcagcac gatgatgaaa ttgcggcgga
     4921 tcaacacgcc caacaggccg agcgagaaga tgatggtgct gaccacgaga taatgggtga
     4981 gcgtgatttc catcagtggc cgtggcctcc gtggtgcgcg cccacggttt ccaccggctc
     5041 ctcaagcgcg ggacccgagg ggccctcgcc gttgcccgac ccgtttgccg gcggcgcgga
     5101 cggctcctgc gattggcccg acatggcggg cgtcttggcc agcacgatcg cgcccacgat
     5161 cgcggccagc aggtagatgc cgatcaactc gaacggcagc aaatagtccc ggaacatcac
     5221 gatgccgatg gccttggtgt ggcccacgcc cagcaccacc tcgggcgtgg cctccccggt
     5281 caacccggcg tagggcgacc gcagcatgag cacggccgcc tgcgcgatga tcgcgatggt
     5341 gatcaggccc atcccgatgt agcgcttgtg aaacacgcgc tcgtcggtct tgaggttcaa
     5401 caacatgatc acgaacaggt acaagaccag gatggcgccc gcgtacacga tgacctggac
     5461 cgcggcgatg aattccgcgt tcagcagcac gaagaacccc gccatgtgca cgagcatcgc
     5521 caccaatgcc aggccgcagt gcacggggct gcgcagcgtg atggtcaaca cgccggacac
     5581 aatggtcacc gcggcgaggt aatagaacgc gagcgcgctc acgccgatca gcccctcctc
     5641 gacggcgcgt gggggaactt gtagcggtac tgccgggccg cttcgtcgct gtcttcctgg
     5701 tgatgggaca ccaggtagct cttggcctcg gcgtggagtt gatcgccgat ctggtagagc
     5761 cggtccttat cgaacatcag gtcgcgcttg ttcatggtgg agaactcgta catcttggtc
     5821 atggccaagg cattgaccgg acaggcctcc acgcaaaacc cgcaaaacac gcacttggtg
     5881 atgtcgatgt agaactcggt cgcgatccgg gtcagcgggg tgacgggatc ttcctcgctc
     5941 acgaccttga tgcagcgcga ggggcacgcc gcctcgcaca gatcgcagcc cacgcaccga
     6001 tccttgccgt cgtcgtagcg cagcagacac aacgcgccgc ggtgcgtgtc cgggatcgcg
     6061 cgcttctcgt gcggatactg aaacgtgacg ggtttgacga acatgtgctt gaacgtggtc
     6121 ttcatcccgt ccaagatttc ccgcagcacc gccttatcaa agagctcttt gagcgtcata
     6181 ctgtcacacg ttcccgtagg ggcacggcat gccgttcccc tacctcacct tcacgaactt
     6241 cacgcggccc gccttgaaca ccggcgactt ggtgaccggg tccaccaaca tcggcaacac
     6301 gtccttgacc gccggatcat tgaaatgctc cggaaaccgg cacgacccgg ccggcagatc
     6361 gagattggtc tcgacgccga cttcaaccga tccgagcggc gacgacacct tcacccgacc
     6421 gttgtcggcc agccccaagg cctcggcgtc cttgagtgag atctggagga gtttcttggc
     6481 attgatctcc agcaacccct tgtcgcgggt ggacatcttg cccgagtgat agagcacctg
     6541 atacagcgac agttcaacgg ggtactcggc attcgtcgcg ccggaggaca ccgcggctcg
     6601 cgcgtaccgc gccgccacgt cggcgctcag tccggccgcg ggacgggtcg cgcccggcat
     6661 ctcgaacgcg gcgggcgcca cctgcaacaa ttcgcgccgg atctcttccg cctcggtgta
     6721 atcgaacgac gcgcccaaca gttgccccag ttcggagaag atttcccaat cggtccgcgc
     6781 ttcgcccgga ggatcgagcg cgcgcacgac cggctgtaac gagccctgcc cgtccagaaa
     6841 cgtgccggat ttctcggccg cgagggccgc gggcagcacc acgtgcgctg cctcgcccat
     6901 ctcggtgagg aacgcgtctt gaacgatcag cagatccagc ttggccaacg cctcgcgcac
     6961 gcccgcgctc gccggcaacg tgcccagggg attctcgccc accgcgtaca aagccttgat
     7021 gcggcccgcg cggatgccat cgaggatctc ggtgagcgtg gcgccattgg tcggcagcgt
     7081 cgtgccccag gcggactcga tgcgcgagcg cgccgcggcg tcgtcgtatc gggacccgcc
     7141 aggcagacga tccgccaacg cgccggtcgc tattgcgccg cgctcgttgt tctcttcgca
     7201 cgtgcgcacc acgccggacc cgggctgccc caccatgccg ctcgccagca tcaagtccgc
     7261 gatccgcaac acgtttgcgt acccgtccgg ccggttcacg atctgctcgc cgagcacgat
     7321 ggcgcgcgtc tcgccggaca ggaacacgcc cgcagcctcc gcgaactgct cgcgcgacag
     7381 cccggtggcc acgcggagcg cgtcccacga caaggccgcc agcgcggacg ccagcacgga
     7441 cacggccgcg ccctgctccg tcgtcacgcg ggccggcacc agaccgctct ccacgatcgc
     7501 cttggtcacc ccctgtacga acgcgccctc ggacccggcc ggggcctgat acgggtacgg
     7561 cgcgagcttg gtcaggttgg tgcgcatgga atgcgccacg atcaattgcg cgtggaagtt
     7621 gctgaacgcg tccttcacgc gcaacgagag aatcggattg gtttcggtca ggtccgaccc
     7681 caccaccagg atcgaccgcg ccatggtcaa atcgcggaac gagagcgtgg gctggaccac
     7741 gcccaacgcg cgctccatgg cttccacgta caccgcgtgg ccgtaccgcg cgctcgaatc
     7801 gatctgattg gtgcccaccg cctggcgcat gaagcgctgg aacgcgtaga gatcctcatt
     7861 ggtacagcgg gccgtgatca atcccgcaat ggcgtcgggt ccgtactcgg acttgatcgc
     7921 ccggaggcgt ttcgcgatct cggccgtggc ctcgaaccag gacgcttcga ccagcttgcc
     7981 gcccttgcgc atcaacggct ttttcaagcg atccgggctg ttcacgtacg cgaagccgaa
     8041 ccggcccttc acgcacaacc cgccgtgccc gtcgaccgtg gaggtgtggt cgtcccactt
     8101 gtccttccac gacaactgcg acgtgacccg caccaccgcg tcgtccttga cctccacccg
     8161 catctggcag ccgtccgcgc agtagttgca cgcggtcatg gtgcggttga gttgccacgg
     8221 cttgtacagg tacttggaga acttgttggt gatcgcgccc accggacaga ccgccagaca
     8281 atccccgcag aactcgcagt ccagcgcgtt gtcgcccttg gccaccacgt gggtgaaccc
     8341 gcccttcttg aagaactgca gggcgtccac catcaccact tccttgcaga tgttgatgca
     8401 ctgcgcgcag aggatgcagc ggttcatgtt gaagtcgagc acggtgctgc gccgatcctc
     8461 gggcaatccc ttttgcttga cctcgccgat ttgcacgatc ccgaagttaa aactcatgtc
     8521 ctgcagctcg cagtgcccgt ccgcgtcgca caccgggcaa tccagcgggt gcgtctgcag
     8581 gtgcttttcg accgccttct tgcgcgcgtc gaacatggtc ttggtgtcca cgcgcacgat
     8641 catcccggcc gccaccggcg aggtgcagga gcgcaccggc gccttcttgc cctcgacttc
     8701 cacgaggcac acgccgcacg acccgaacgg gttgaacgtg tagtggtaac acatgctggg
     8761 gatggtcttg cccatcccca cgatcgcgtc gtagatcgac gtgccttccg gcacgctcac
     8821 gacctcgtcg tcgatcgtga gctgcacgtt ggctttgtcg gtggtaatcg aaatcagttc
     8881 agccatataa atcctagaag ttcaacgttc aagggttcaa ggttcaacga gtcgagtcct
     8941 tcacgttgaa cgattgaacg ttgaacagtt tgaacgctca ttatctgtcg cattcgccca
     9001 tgacaatgtc atacgtccca aagatcgtaa tgacgtcggc aatcatgtac ccccgcgcca
     9061 tctgatcaaa cgcgcccatg tggatgaatg agggggcgcg gatcttcagg cggtagggtt
     9121 tgccttcccc gttactcacg atgtaaaagc ccaactcgcc cttgtgcgcc tcggtgccgc
     9181 agtagatctc tcccttgggc gtgggaaaac ccacggagaa gaacttgaac tgcatgatca
     9241 tgctctcaag gttcgtgaac acgcggtcct tggtgggcgc gaccacttgc ggcacgtcgg
     9301 ccatgatcgg gccgtcgggc aatttgtcca gacactgccg gatgatcttg ctgctctcct
     9361 tcaactcttc gatccgaatc cagtaccggt cgtaggtgtc gccgttgacg ccgatcggca
     9421 cgttccactc cacgtcgccg tacgcgccgt agggctcggc cttgcgcagg tcgtactcca
     9481 cgcccgagcc gcgcaacgtg ggaccggaca gaccaaagct caacgcgtct tcggccgaga
     9541 tcaccgcgat gcccttggtg cgcgcgatcc agatccggtt cttttcgagg agcacgttgt
     9601 actcttcgac tttggccgga aagtctttga taaacgcgtt gaacccgtcg atgatgtcct
     9661 gggtgaagtc gcgctccacc ccgccgatgc ggtaccaact cgtggtcagg cgcgcgccgc
     9721 agagtttgtc gaacaggtcc agcagcacct cgcgctcgcg gaacgtgtag aagaacacgg
     9781 tcatggcgcc gatgtcgagc gcctgcgtac ccagccagaa caggtgcccg atgatgcgct
     9841 gcacctcggc cacgaacgtg cgcaagtact cggcgcgcgg cgtcaccgtg atacccatca
     9901 gcttttccac ggcccggacg tacgcgaa
//
LOCUS       DBNGTT010000194         4212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1188708, whole genome shotgun sequence.
ACCESSION   DBNGTT010000194 DBNGTT010000000
VERSION     DBNGTT010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1188708"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..2915)
                     /gene="hrpB"
                     /locus_tag="AC16BC_04415"
     CDS             complement(387..2915)
                     /gene="hrpB"
                     /locus_tag="AC16BC_04415"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HIR3240893.1"
                     /translation="MTTLPIHDILPALRDALRAQSSVVLQAPPGAGKTTHVPLALLDE
                     PWLAGKSLLMLEPRRLAARAAAGRMAEILGESVGDTVGYRIRFEAKVSAKTRIEVLTE
                     GLLTRRLQSDPGLDGVGLVIFDEFHERHLHADLALALCLESRRVLREDLKLLVMSATL
                     DGEAVATVLGKAPVITASGRQFPVDVRYAATDARDRLPDTVAQAVLRALERDPGDVLA
                     FLPGAWEIRRAQQVVENELRGQPIDVHPLYGDLPWEAQDRAIRPGARRKIILATPIAE
                     TSLTIEGIGIVIDSGFVRVPEFDPGSGLTGLATHRISKASAEQRAGRAGRLGPGVCER
                     LWTEHTHRGLIDRPVPEIRAADLAPLALELAQWGIRDAGSLAWLDPPPAGALAQARGL
                     LTQLGALDADGVITPLGRAMAALPLHPRLAHMVHLADAQGLGKAACDVAALVSERDVF
                     TGKETRSCDVLERVEALNAFRSKGREAARAAGADPAACQRVDQAARQYQRVLGSRRAE
                     RDASADDIGILLALAYPDRIARQRAAGDIRYVLASGRGARLPAWEHRLRPPWLVAAHL
                     DAGEVEGTIHLAAALDLDAWRRAVPDAVTTESIVQWDERLERVVARRDERIGELVIES
                     VPLKEPDPEQVRAAMLEGIRRLGLDALPWTTAAREWQARMLSLRHWLPDEAWPDMSDA
                     ALTATLADWLGPFLDQITRREHLNRLDLLAALGSRLDWKQRQRLDEGAPTHLTVPSGS
                     RVRLEYRPGESPVLAVKLQELFGLADTPRIAWGRVPVTLHLLSPARRPIQVTQDLRGF
                     WDRTYPEVKKALKGRYPKHPWPDDPWNAVPTARTKRGSANPRRA"
     gene            complement(2947..>4212)
                     /locus_tag="AC16BC_04420"
     CDS             complement(2947..>4212)
                     /locus_tag="AC16BC_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3240894.1"
                     /translation="ERLARDKEALSQIVTREMGKVIAEARGDVQEAVDMAYYMAGEGR
                     RLAGQTVPSELPNKDAKSVRVPLGVCGLITPWNFPVAIPSWKILPALVAGNTAVIKPS
                     ELTPLCATRFVQILQDSGLPPGVLNLVHGYGEDAGGALVSHPDVNAVSFTGSSAVGEQ
                     VEAACGRLHRPVCTETGGKNPLIVMEDADLDLAVEGALWGGFGTSGQRCTAASRVIVH
                     QAAYDAFLARFVDAAKRLRLGSGLDPKTHVGPVISADQKRMVLEYIAIGKKEGAKLLC
                     GGHAVTDGALANGHFIAPTVFGEVAPAMRIAQEEIFGPVVSVIKARDYAHAIEIANNV
                     AYGLSAAIYTRDVNTSAMAERDLDTGIVYVNASTIGAEIQLPFGGTKRTGLGPREAGG
                     RGGALELYTKWKVIYRDFSGKLQKAQIDA"
BASE COUNT          649 a         1484 c         1389 g          690 t
ORIGIN      
        1 ctgactattt ggccgacgac ccatagcact agaccatcaa acggtgtcat ttttgaaacc
       61 taactacaat tcgacatccc aattatttcg acaaaccaag cgcctaccgc ttcccaaccg
      121 ccccagcagc tatacaacta aattgctgag tctcttccag attacatgca gttacgaccg
      181 cgacctctat caaccgctgg acgctgttat gacgcatctc acgcttccta acacgccgcg
      241 atccattatc tccattccct gaagtgtgtc tcgccatcca gccggatcgg gaggcccttc
      301 gtgactctcg gcgttgagga acgcgccggt tgcgatcagg atgatcggta caggtagcgg
      361 gacaatatct gagtcgcgtc tctcggtcaa gccctgcgcg gattggcgct gcctcgtttc
      421 gtgcgcgcgg tgggcacggc gttccaggga tcgtcgggcc aggggtgttt ggggtagcgg
      481 cctttgagtg cttttttcac ttcggggtag gtgcggtccc agaagccgcg gaggtcctgg
      541 gtaacctgga tcgggcggcg cgcgggggac aagaggtgca gggtgaccgg cacgcggccc
      601 caggcgatgc gcggggtgtc ggccaggccg aacagttcct ggagtttcac ggccaagacc
      661 ggggattcgc cgggccgata ctccaggcgc acgcgcgagc cgctcggcac cgtgaggtgg
      721 gtgggcgcgc cttcgtcgag tcgttggcgt tgtttccagt ccagccgcga gccgagcgct
      781 gcgagcaggt cgaggcgatt gaggtgctcg cgtcgcgtga tctgatcgag gaacgggccc
      841 agccagtctg cgagcgttgc ggtcagcgcg gcgtctgaca tgtcgggcca ggcttcgtcc
      901 ggcagccagt gacggagcga cagcatgcgc gcctgccact cgcgcgcagc agtggtccac
      961 ggcagtgcat ccaggcccag gcggcggatt ccctcgagca tggccgcgcg gacttgctcc
     1021 gggtccggtt cctttaacgg cacgctctcg atcacgagtt cgccgatgcg ttcgtcgcgg
     1081 cgcgcgacca cgcgttccag ccgctcgtcc cactggacaa tggattcggt cgtcaccgcg
     1141 tccgggacgg cgcggcgcca cgcgtccaag tccagggccg cggcgagatg gatggtgccc
     1201 tcgacttcgc ccgcgtccag gtgcgcggcc accaaccacg gtggacgcaa gcggtgctcc
     1261 cacgcgggca ggcgcgcgcc gcggcctgag gccaggacgt agcggatatc tccggctgcg
     1321 cgctgccgcg cgatgcgatc cgggtaggcc aaggcgagca agatgccgat gtcgtccgcg
     1381 ctcgcgtcac gttcggccct tcgggacccc agcacgcgct ggtactggcg cgcggcctga
     1441 tccacgcgct gacacgcggc cgggtcagca ccggcggcgc gggcggcctc gcggcctttt
     1501 gaccggaacg cgttcaacgc ttccacccgt tccagcacgt cgcacgatcg ggtctccttg
     1561 cccgtgaaca cgtctcgctc ggacaccaat gccgccacgt cgcacgccgc cttgcccaac
     1621 ccttgcgcgt cggccaaatg caccatgtgc gccaatctgg ggtgcaatgg caacgcggcc
     1681 atggcgcgtc ccaagggcgt gatcacgccg tccgcgtcca gggcgcccag ttgcgtcagc
     1741 aggccgcgag cctgggccaa tgcgccggcg ggcggagggt ccagccacgc cagcgacccg
     1801 gcgtcgcgaa tcccccactg cgcgagttcc aaggccagcg gcgccaggtc cgcggcgcgg
     1861 atctcgggga caggccggtc gatcagaccg cggtgcgtgt gctcggtcca caaccgctcg
     1921 cacacgccgg gtcccaaccg gcccgcgcgg cccgcgcgct gctcggccga ggccttggaa
     1981 atccggtgcg tggccaaacc cgtcaacccg ctgccgggat cgaactccgg cacccgcaca
     2041 aagcccgagt cgatcacgat gccgatgccc tcgatcgtca aactggtctc cgcgatcggc
     2101 gtggcgagta tgatctttcg gcgcgcgccc ggccggatcg cgcggtcctg cgcttcccag
     2161 ggcagatcgc cgtacaacgg atgcacgtcg atcggttgtc cgcgcaactc gttctcgacg
     2221 acctgttgcg cgcgccgaat ctcccatgcc ccgggaagga acgcgagcac gtccccaggg
     2281 tcccgctcca gcgcgcgcaa cacggcctgc gccacggtgt ccggcagccg atcccgcgcg
     2341 tccgtggccg cgtaccgcac gtccaccggg aattggcgtc ccgacgcggt gatcaccggc
     2401 gccttgccca acaccgttgc cacggcttcg ccgtcgagcg tggcggacat caccagcagc
     2461 ttgagatctt cgcgcaggac gcgccgactc tccaggcaga gcgccagcgc caggtccgcg
     2521 tgcaggtggc gttcgtgaaa ctcgtcgaag atcacgagcc ccacgccatc gagacccgga
     2581 tcggattgca ggcgtcgcgt gagcaggcct tcggtcaaca cctcgatgcg cgtcttggcc
     2641 gacaccttgg cctcgaaccg gatgcggtac cccacggtgt cgcccacgga ctcgcccagg
     2701 atctcggcca tgcggcccgc ggccgcgcgc gcggccaggc ggcggggctc caacatcagg
     2761 agagatttgc cggccaacca cggttcatcc agcaatgcca agggcacgtg cgtggtcttg
     2821 cccgcgccgg gcggcgcctg cagcaccacg gaggattgcg cgcgaagcgc gtcgcgcagc
     2881 gcggggagga tgtcgtggat gggtaacgtg gtcatcggcc gaccggcgtg ggcagccgga
     2941 tctgcgtcac gcgtcgatct gcgccttttg cagcttgccg gaaaagtcgc ggtagatcac
     3001 cttccacttc gtgtacaact ccagcgcgcc cccgcggccg ccggcctcgc gcgggcccag
     3061 gccggtgcgc ttggtgccgc cgaacgggag ctggatttcc gcgccgatgg tcgaggcgtt
     3121 gacgtacacg atgccggtgt ccaggtcgcg ctccgccatg gcgctcgtgt tcacgtcgcg
     3181 cgtgtagatc gcggcggaca ggccgtaggc cacgttgttg gcgatctcga tcgcgtgcgc
     3241 gtagtcccgc gccttgatga ccgagaccac cgggccgaag atttcctcct gcgcgatccg
     3301 catggcgggc gccacctcgc cgaagaccgt gggcgcgatg aagtgtccgt tggccaacgc
     3361 gccgtcggtt acggcatggc cgccgcacag gagctttgcg ccttctttct tgccgatcgc
     3421 gatgtactcc agcaccatgc gcttctggtc cgcgctgatc accggcccca cgtgggtctt
     3481 ggggtcgagc ccggacccca atcgcaggcg cttggcggcg tcgacgaacc gcgcgaggaa
     3541 cgcgtcgtac gcggcctggt gcacgatcac gcggctcgcc gcggtgcagc gctggccgct
     3601 ggtgccgaac ccgccccaca acgcgccttc aaccgccagg tccaggtccg catcctccat
     3661 cacgatcaag ggattcttgc ccccggtttc cgtgcagacc ggccggtgca gtcgcccgca
     3721 cgcggcttcg acctgctcgc ccaccgcgga cgatccggta aacgacaccg cgttgacgtc
     3781 gggatggctc accagcgcgc cgcccgcgtc ctcgccgtat ccgtggacca ggttcaggac
     3841 cccgggcggc agccccgaat cttgaagaat ctgcacgaac cgcgtggcgc agagcggggt
     3901 caattccgac ggcttgatca ccgcggtgtt gcccgcgacc aacgcgggca ggatcttcca
     3961 ggacgggatc gccaccggaa agttccacgg cgtgatcagg ccgcacacgc cgagcggcac
     4021 gcgcacggac ttggcgtcct tgttgggcag ctcggagggc acggtctggc ccgcgaggcg
     4081 gcggccttcg cccgccatgt agtaggccat gtccacggcc tcctgcacgt ccccgcgcgc
     4141 ctccgcgatc accttgccca tctcgcgggt cacgatctgc gacagagcct ccttgtcacg
     4201 cgcgaggcgc tc
//
LOCUS       DBNGTT010000195         3196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_167268, whole genome shotgun sequence.
ACCESSION   DBNGTT010000195 DBNGTT010000000
VERSION     DBNGTT010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_167268"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="AC16BC_04425"
     CDS             <1..636
                     /locus_tag="AC16BC_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240895.1"
                     /translation="ALLASRLESSGGEVVRTPIAALGTGTSPFVETDGRRLDARVLIV
                     NSRGRPGVAGAEERGAGAQTTAAFSVPSSCVPDAMASFLLAADGDGVWSLSRRRGEAG
                     GPAPSEVLTVSCRGRVDGKDAARIEDRLRGLMPFAAGRLTFEGMLRDEDVPDPVEAKM
                     WGEVSWRARRFGWAQAGRTPVWWLADACEPWLGDAGPYRTALAVDRLLRLG"
     gene            681..1709
                     /locus_tag="AC16BC_04430"
     CDS             681..1709
                     /locus_tag="AC16BC_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HIR3240896.1"
                     /translation="MWALAGVLLLGRASALAADDALERARDALERGDLAAAAATAGAF
                     IASSPGDPRLADAQRLLGLARERAGALGDAWQQYTLFLTNFPSHPARDDVAERIDRLL
                     RHIRERADVLPNRWQPVPSWDGPRSPSNDTALVVTFRDVAEVDRVAPRLTRAALDGAR
                     IWVRASLGAPDGVFDPFDVETVTRREQWFHAMASRPIEGFVIDGLGLGPDARPAAAAR
                     AWAELARVIPETPEGRERLAWTWAGTRARASAAALQRFVAAAEGVAPRIGWVVRVSVD
                     VVLRPEQAVREAGEDWGELRIAAPRAVWAMDIPAGLESRLIKAAMDNGSRPPLARRTQ
                     GDGFVLLK"
     gene            1909..2301
                     /gene="gcvH"
                     /locus_tag="AC16BC_04435"
     CDS             1909..2301
                     /gene="gcvH"
                     /locus_tag="AC16BC_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR3240897.1"
                     /translation="MVPDDLRYSTDHEWVKVEGKQALIGITDYAQGELGDIVYIELPE
                     VGIEVAAEEEVTEIESTKTTSPVLAPISGKIIEVNEELKESPQLINEDPYGKGWIAVM
                     TIASADDVDALMSAKEYEEFIEEGEEEE"
     gene            2371..>3196
                     /locus_tag="AC16BC_04440"
     CDS             2371..>3196
                     /locus_tag="AC16BC_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3240898.1"
                     /translation="MPKRLLVLGGGPGGYVAALRAAHLGAQVTVIESRGLGGACLHRG
                     CIPTKALVATADVLRTVRRAKEFGVRGSDQVVVDLAEVVARKNRLVQSQTQGIEQLFK
                     AAKVTLVRGVGALTGPRSALVRDAQGRTRTIEADAVIVATGSEPARPAEFPFDGERVL
                     TTDDALDLTRLPASVVIVGGGVTGCEFACIFRALGADVTVIERLPRVVATEDEAVSSL
                     LLRELKKQGIAVRLDTGVTQVESSARGVRIATSSGDPVSAECVLVAVGRSLNSAGIG"
BASE COUNT          434 a          987 c         1275 g          500 t
ORIGIN      
        1 gcgctgttgg cgtcacgtct cgagtcgtcc ggcggggagg tggtgcggac gccgatcgcc
       61 gcgctcggca cggggacgag tccgttcgtg gaaaccgacg ggcgtcggct ggatgcccgc
      121 gtgctgatcg tgaactcccg gggccggccc ggcgtggccg gggcggagga gcgtggagcc
      181 ggagcgcaga ccacggccgc gttttcggtc ccctcgtctt gtgtcccgga tgcgatggcc
      241 tcgttcctgc tcgccgccga tggcgacggg gtctggtcgt tgtcgcgccg tcgcggcgag
      301 gccggcgggc cggcgcccag cgaggtgttg accgtgtcgt gtcgagggcg ggtggacgga
      361 aaggacgcgg cacggatcga ggatcgactg cgggggctga tgccgtttgc cgccgggcgt
      421 ctgaccttcg aggggatgct tcgcgatgag gatgtccctg atccggtgga ggcgaagatg
      481 tggggcgagg tctcatggcg cgcacggcgt ttcggctggg cgcaggcggg gcggaccccc
      541 gtgtggtggc tggccgacgc gtgcgagccg tggctcggag acgcgggtcc gtatcggacc
      601 gcgctcgcgg tggatcggct gcttcggttg gggtgaggcc gcgctcggtg agccacggga
      661 catggcgccg cggaggagcg gtgtgggcgc tcgcgggcgt attgctgctc ggtcgggcct
      721 cggcgcttgc ggcggacgat gcgttggagc gcgcgcgcga cgcgctcgag cggggcgatc
      781 ttgccgcggc cgcggccacg gccggcgcgt ttatcgcctc ctctcccggg gacccgcgat
      841 tggcggacgc gcaacgcctg ctggggcttg cgcgcgagcg ggcgggggcg ctgggggacg
      901 cttggcagca gtacacgttg ttcctgacca actttccctc gcatccggcg cgtgacgacg
      961 tcgcggagcg catcgaccgt ctgttgcgac acatccggga gcgggccgac gtgcttccga
     1021 accggtggca gccggttccc tcgtgggacg ggccgcgctc gccctcgaat gacacggccc
     1081 tggtcgtgac gtttcgggac gtggccgagg tggaccgcgt ggcgcccagg ctgacgcggg
     1141 cggcgctcga cggcgcgcga atctgggtgc gggcgtcgct gggcgcgccg gacggggtgt
     1201 ttgatccgtt cgatgtcgag acggtcacgc ggcgcgaaca atggtttcac gccatggcct
     1261 cacggccgat cgaagggttc gtgatcgacg gactcgggct ggggccggac gcgcgtcccg
     1321 cggcggccgc tcgcgcgtgg gcggaactgg cccgggtgat cccggagacg ccggagggcc
     1381 gcgagcgtct ggcctggacg tgggcgggga cgcgcgcccg cgcgtcggcg gcggcgctcc
     1441 aacgcttcgt ggcggcggcg gaaggggtgg ccccgcgcat cggatgggtg gtccgcgtgt
     1501 cggtggatgt cgtgctcagg cccgaacaag ccgtgcgtga ggcgggggag gactggggcg
     1561 aactccgcat cgcggcgccg cgcgcggtct gggcgatgga tatccccgcg ggccttgagt
     1621 cccggctcat caaggcggcg atggacaacg ggtcacgccc gcccctcgcg cgacggaccc
     1681 aaggcgacgg gttcgtcttg ctcaagtgac ggccgacgag acggggcgcg gacgacggcg
     1741 ccgtgaggaa ggcaccgatc gtcatggcga gccctcggcc ggcttcttgc gccgattcgc
     1801 cgcgcacgct cgttgacaag caaaaaccgc gtcgggtagt atcgggcctt ctttgcgcgc
     1861 cgccgcggtg ttccggcgcg ccgcgccggc gacagccagg aggacagcat ggtacccgat
     1921 gatttgcggt attccactga tcacgagtgg gtgaaggtgg aagggaagca agcgctgatc
     1981 ggcatcaccg actacgcgca gggcgaattg ggcgacatcg tgtacatcga actcccggaa
     2041 gtcgggatcg aggtcgcggc cgaggaggag gtcaccgaga tcgaatccac caagaccacc
     2101 tcgcccgtgc tcgcgcccat cagcggcaag atcatcgagg tcaacgaaga gctcaaggag
     2161 tcgccgcaac tgatcaatga agatccgtac ggcaagggct ggatcgccgt gatgacgatc
     2221 gccagcgccg atgacgtgga cgcgctcatg agcgccaagg agtacgaaga attcatcgag
     2281 gagggtgagg aggaggaata accctccgcc gctccgatcg gttggcccgg ggcccgtccg
     2341 ccgcgtcccg gattcgctgt acgacttccc atgccaaagc gactgctcgt gctgggtgga
     2401 ggccccgggg gctacgtggc cgcgcttcgt gccgcgcacc tgggcgcgca ggtcaccgtg
     2461 atcgagtcgc gcggcctcgg cggcgcgtgt ctgcaccgcg gctgcattcc gaccaaggcg
     2521 ttggtggcca cggcggacgt gttgaggacc gtgcgccgcg ccaaggagtt cggcgttcgg
     2581 ggctcggatc aggtcgtggt ggacttggcc gaggtggtgg cgcgcaagaa ccgcctggtt
     2641 caatcgcaga cgcaaggcat cgaacagctg ttcaaggccg ccaaggtgac gctcgtgcgc
     2701 ggggtgggcg cgctgaccgg gccgcgatca gcgctggtcc gcgacgcgca ggggcgcacg
     2761 cgcacgatcg aagccgacgc ggtcatcgtg gccacgggct cggagcccgc gcggcccgcc
     2821 gagttccctt ttgacggcga gcgcgtgttg accaccgacg acgcgctgga cctcacgcgt
     2881 ttacccgcga gcgtggtgat cgtgggcggg ggcgtcacgg ggtgcgagtt tgcgtgcatc
     2941 tttcgcgcgt tgggcgcgga cgtcacggtg atcgagcggc tcccgcgcgt ggtggcgacc
     3001 gaggacgagg cggtgtcctc gttgttgctg cgggagctga agaagcaggg gatcgcggtg
     3061 cgtcttgaca ccggggtgac gcaggtcgaa tcgtcggcgc gcggcgtccg gatcgccacg
     3121 tcgtcgggcg atcccgtgag cgcggaatgc gtcttggtcg cggtcgggcg gtcgctcaac
     3181 agcgcgggca tcggcg
//
LOCUS       DBNGTT010000196         9837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2722841, whole genome shotgun sequence.
ACCESSION   DBNGTT010000196 DBNGTT010000000
VERSION     DBNGTT010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2722841"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(820..2262)
                     /locus_tag="AC16BC_04445"
     CDS             complement(820..2262)
                     /locus_tag="AC16BC_04445"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3240899.1"
                     /translation="MKKILIVEDHDSLRKGIAEAFQRSGHRVTEAPDAETAIRLIGEE
                     FFGMVITDVSLPGKSGLEVLKTAKATNPLTTVIVMTAYGTVENAVEAMRLCCDDYIQK
                     PFQIEELELKVSKLLEHQAVRQELDDLRGRIGWPNQLVGTSPALRQLLSIIEKVARGN
                     STVLIEGETGTGKELIANAIHTASPRSGRSLVKVNCAALPEPLLESELFGHERGAFTG
                     AERQRIGRFELANGGTLFLDEIGDMSLPTQAKLLRVMQEQTFERVGGEKTISVDVRII
                     AATNKHLETEVRERRFREDLFYRLNVVTITLPPLRERPEDIPLLARHFLERYARELGK
                     PVGAIEPAACKVLDGYQWPGNIRELENTIERAVLMAEGDTLRVSDLGLASQPAHGTAA
                     GHSALGKGPTVAIHSPGGRAPASNPRSRHLDDVEKSTILDALVESNWVQKDAARILGV
                     SPRVLNYKIKSHGITHPSWLKHRPPETRTA"
     gene            complement(2259..3344)
                     /locus_tag="AC16BC_04450"
     CDS             complement(2259..3344)
                     /locus_tag="AC16BC_04450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3240900.1"
                     /translation="MDRTTVLHHVFAQLRSGIVAVDRGKRVVLMNAAAGAILGLDPAN
                     AEGRPIDEALTAHPNLVGFLADALNLNTVLDRAEMSIGGPHCRTLGVTSTPLPAATGH
                     EGGLVVIFKDLTQVERQEEQAQLKERLMALGQMAAGLAHEMRNPLAGIKVAVGLLRKT
                     LKADPEVDQKLHSIMGDVNRLNQSVTDCLAFARPIRLEPKYVQINDVLEKVLGQTVSL
                     EYTYPFQVARQFGADLPKTWVDPQQLEIVFFNLINNAIDAVRQRHGTITISTAARRDA
                     DTGQDSLVTTIEDNGEGIPRDRLPKVFMPFFTTKGKGSGLGLANAKKIVDHLRGTIEL
                     SSEPGRGTCFAVHLPVLTTPEQGARAA"
     gene            complement(3518..5827)
                     /locus_tag="AC16BC_04455"
     CDS             complement(3518..5827)
                     /locus_tag="AC16BC_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240901.1"
                     /translation="MIRVPRAVTLVLPLILALALGTACSKDPAAQKAAHLSKAEQYMD
                     DRKYAEAVIEFRNALKIDPNDAKAHFRLAVAYLNHDATSYAQQAFIALGKAIELDPEF
                     LDAHLKMGELLVLGQRFDKAREKAEFILAKDPQHLGALMLLANVELATDHPDAALKTM
                     AKAKTAHPGESKPWLAEAAIYARMRNYAAAEVSVKEAARLAPQDTEPVLGLAAIYRAQ
                     GKTAEAETQYRSAIALKPDNLPLYLVLAQFHALNGDQTKADAAYREAVAKFPTQQGAR
                     VAYARYLIAANRADDAATQYRAVIKDYPRDAAAKKQLANLLLDHQHPDDARALVEDLK
                     AERSGDADVLYLDARLLILDKQVDRAVTTLQQAVASDPTHADAHRALGILYARRGDLQ
                     QAQTELAEAVKFRPTAWDARLALAELHLRSKSPDLALEQARAVLAAVPRYPPALQMAG
                     DALLASGKPREAMEQYDALITNVPTSYVGYYRKGIAYRALNKPQAAEAQFRLALDKAP
                     GSVDVVQQVAAMLLQRKDRSGALRLIEGQLDKAEQKAPLHHLLGTVYVALDDATNAET
                     QFKRAIDENKGFLPSYEALGTLYTRTREPAKVIAQYRAMAEAAPDNPSPHFLLGMTYE
                     AQQRSDEAIAEYERALKIDARFAPAANNLAWLYADQGKNIDVALGLAQTAMEQLPNDP
                     GVADTLGWIYYKKSAYLKAIALLRESSDKLPDNATVRYHLGTALYANGDHKAAEQELT
                     KGLRLSPSAPWASDAKKTLAAIQAPTTSN"
     gene            6023..7507
                     /locus_tag="AC16BC_04460"
     CDS             6023..7507
                     /locus_tag="AC16BC_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR3240902.1"
                     /translation="MKRSRNIVLALILVGVGVVLGVTMASDLGWLPLGEAQNETKAAR
                     PVLTGGLPNFVEVSKAVKPAVVNISTTRMVKSEEGQGPGNPFFEDPFFRRFFGDEFYR
                     QFDTPRQRKEQSLGSGVIVDGGYIVTNNHVVAKADEIKVVMSDKREFKGTLVGTDPKT
                     DLAVIKVPATGLQTIPWGDSTTLEVGEYVLAVGNPFGLNQTVTMGIVSAVGRANVGIA
                     DYEDFIQTDAAINPGNSGGALVNVKGELVGINTAIFTRSGGYMGIGFAVPSQMVKSVI
                     ESLVSKGKVVRGWLGVTIQEVSPELAKQFGLKEARGALVSDILEGSPADKAGIKRGDV
                     VIEVDHKSVENASQLRNLVAALSVGSKASVKVIREKKEKVFDVVIGEQPKDLAGKSGE
                     PSEEDGGEGALAGIGVHDLSPEFAKRFGLPEDEEGVVVTRVEPGSAAEDAGVQRGDLI
                     LEINRQPVQTTREFSRIAREIGKDDSILLLVNRQGQALFITITP"
     gene            7548..8573
                     /locus_tag="AC16BC_04465"
     CDS             7548..8573
                     /locus_tag="AC16BC_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR3240903.1"
                     /translation="MFASLVLGAVLARGGGAQGLQALIVGLAIGLALAAVPLGLDALA
                     AAWSWQRVAGSVLGGVAGIAASGVVLWATSRVSLGPTMARYWPVAVLVALGYAGWRFG
                     GQWAAMLMFAASPPSSAPRMPSNVVAGRKLLDTSVIIDGRVADLCDTGFLEGNLLVPQ
                     FILQELQHIADSSDTLKRARGRRGLDILHRIQNMPGVTVTILDEDVPQVKDVDAKLVA
                     LAKKLDAKIVTNDLNLNKVAGLQGVRVLNTNELSNALRPVVLPGEPMRVLVQREGKET
                     GQGVAYLDDGTMIVVDDGRKYIGRHVEVVVTSVLQTAAGRMIFTRLREDADRGESGVR
                     PERSLSA"
     gene            8570..9310
                     /gene="ispD"
                     /locus_tag="AC16BC_04470"
     CDS             8570..9310
                     /gene="ispD"
                     /locus_tag="AC16BC_04470"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR3240904.1"
                     /translation="MSVVAVIPAAGSGSRLGRGSPKQYLPLAGMPVLARTLAVFDAST
                     AIDDIVVVVPPGDESRCRETAVEPYGWKKIRAIVPGGAVRQESVACGLAAVGADAAIV
                     VVHDAARPLVTDDLIRRVVAAAAATGAAIAALPVVDTLKRRVGEKGLVTTVDREGLWT
                     AQTPQAFRVGLLRDAVARARADGFVGTDDASVVERLGLPVALVEGEASNFKITRAEDL
                     GRAEEILLHARDRASRPAALDRVGSRFS"
     gene            9310..9795
                     /gene="ispF"
                     /locus_tag="AC16BC_04475"
     CDS             9310..9795
                     /gene="ispF"
                     /locus_tag="AC16BC_04475"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR3240905.1"
                     /translation="MRIGIGYDVHALVAGRPLVLGGVTIPSERGLDGHSDADALLHAV
                     ADALLGAASLGDIGQHFPDSDPAYRNISSLRLLEAVVERLEEHGFQVGNVDAVVIAES
                     PKLAPYIPRMRTAMAKALRVPETAVSVKATTHERLGSLGRGEGIAAQAVCLLNGKGTP
                     R"
BASE COUNT         1647 a         2804 c         3384 g         2002 t
ORIGIN      
        1 tatggggggg ccataattcc cgggcgttgt cccgtagtaa attttgtagc ccagcaaatc
       61 tggttcggta ttcgggctcc aggacaacgt cgcttctccg gccatgaccg atgtcggcca
      121 gcagagaagc gctacgaata gcaacgacaa gagtagcgag ccgtatccat gcaagtcatg
      181 gcgcatgaaa ctcccccggt cgttcatctt ccaaggtggg cgattcatat tcccgggttc
      241 atccgattgt cgtgtcggcc tgttcccgca tcctgctgac cggtacatat cgaggctccc
      301 tgttctcggc ggtatctagc aaatgccgag ccagctcagt ctcggtgtgc aggagagcgg
      361 aatttgatgt tttggcggtg ttttggctgg cgttcatgac gattcggcga gtctgacgac
      421 gaaattgcaa tgctggttac agattcaggg ggcagaattc ggcagtcgct gctgagtttc
      481 cagcagggtg gagattgatg tggggcgttc ggtggcaatg tcgatggccg gttttgtggc
      541 gtcctgctct ggaaccaaag ggtgatgatg cgccccgttg agtgacgata ttcattcagg
      601 agctgatgca cgctgtcgtc atgcttctcg acgaccgtcc catcggttgt gaaaagacgg
      661 gagtaaccgt tggccttttg aatattgttc ttatctgggt ctggatgcat tactcaagca
      721 gaactacacg tcgtcaattg ctcggtgacg gagaacagta acgaaactgg tcggtgaatt
      781 caagaagtcc ttgggcaatt gtttcgcttg agtggggtgt tatgcggtcc gtgtttctgg
      841 cggccggtgc ttgagccagg aggggtgggt gatgccgtgg ctcttgattt tgtagttgag
      901 gaccctgggg ctgactccga gaatgcgcgc cgcgtccttc tgcacccagt tgctctcgac
      961 gagtgcatcc agaatggtcg atttttcgac atcatcaagg tgtcgcgaac ggggattgga
     1021 tgcgggcgcg cgtcctcccg gtgaatggat ggcgacagtc gggccttttc ccagggccga
     1081 atgaccggcc gccgtcccgt gagctggttg agacgccagt cccaagtcgc tcacgcgaag
     1141 ggtgtcgcct tcggccatga ggaccgctcg ttcgatggtg ttctccaact cgcggatgtt
     1201 tcccggccac tggtagccgt ccaagacttt acatgccgcc ggttcaatcg cgcccacggg
     1261 tttgcccagt tctcgcgcgt agcgttccag gaaatgccgg gcgagcaggg ggatgtcttc
     1321 gggccgctcg cgaagcgggg ggagcgtgat cgtcaccacg ttgagccgat agaacaaatc
     1381 ctcccggaat cggcgctccc gcacttcggt ttcaagatgt ttgttggtgg cggcgatgat
     1441 gcggacgtcc acactgatgg ttttttcgcc gccgacccgc tcaaacgtct gctcctgcat
     1501 cacccgcagt aatttggctt gcgtgggtag gctcatgtcg ccgatttcgt cgaggaacag
     1561 ggtcccgccg ttggccaact caaaccgtcc gatccgctgt cgctccgcgc cggtaaacgc
     1621 gccgcgttcg tggccgaaga gctcgctttc caagagcggt tccggcagag ctgcgcaatt
     1681 gactttgacc agactacgac cgcttcgcgg gcttgccgtg tggatggcat tggcgatcag
     1741 ttccttgccg gttccggttt caccttcgat cagcaccgtg gaattgcctc gggcgacctt
     1801 ttcgatgatc gacagcagtt gtctcagggc ggggctggtt ccgaccagtt ggttgggcca
     1861 gccgatacgg ccgcgcagat catcgagttc ctggcgcact gcttgatgtt caagtaattt
     1921 cgagactttc agttcaagtt cctctatttg aaatggtttc tgtatgtagt catcgcagca
     1981 taaacgcatc gcctcaactg cgttttccac ggtgccgtag gccgtcatga cgatgaccgt
     2041 ggtgagggga ttggtggctt tcgcggtctt gagcacctcc agcccgctct tgccgggaag
     2101 cgacacgtcg gtgatcacca tgccgaagaa ttcttccccg atcaaacgga tcgcggtttc
     2161 tgcgtcgggc gcttcggtca cccgatgtcc cgagcgctgg aaggcctcgg cgatgccttt
     2221 gcgtaacgag tcgtgatctt cgacgatcag gatttttttc acgccgcccg agctccttgt
     2281 tccggtgtag tcaacaccgg gaggtggacg gcaaaacacg ttccccgtcc cggttcgctg
     2341 ctcaattcga tcgtgccgcg gaggtgatcc acgatttttt ttgcgttggc gaggccgagg
     2401 cccgatccct tgcctttggt ggtaaagaac ggcatgaaga ctttcgggag tcggtcgcga
     2461 gggatgcctt cgccgttgtc ctcgatcgtc gtgaccagac tgtcctggcc cgtgtcggcg
     2521 tcgcgccggg cggcggtcga gatcgtgatc gtgccgtgcc gctgccgcac cgcatcgatc
     2581 gcgttgttga tgaggttgaa aaacacgatc tcgagttgtt gcggatccac ccacgttttg
     2641 gggaggtccg cgccaaattg ccgcgcgacc tgaaacgggt acgtgtactc caacgacacg
     2701 gtttggccga gcactttttc cagcacgtcg ttgatctgga cgtatttggg ttccagccgg
     2761 atgggcctgg caaacgccag acagtcggtc acgctctggt tgagacgatt cacgtcgccc
     2821 atgatcgagt gcagcttttg atcgacttcc gggtcggcct tcagcgtctt tcgcaacaag
     2881 ccgaccgcga ccttgatgcc ggccagcgga ttgcgcattt cgtgcgcgag ccccgcggcc
     2941 atttgtccca atgccatgag tcgttctttc agttgcgcct gctcttcctg gcgctccacc
     3001 tgggtcaggt ccttgaaaat gacgaccagt ccgccctcgt gaccggtggc ggcgggcagc
     3061 ggcgtggaag tgacgcccag ggtgcgacag tgaggcccgc cgatggacat ttcggcccgg
     3121 tccagaacgg tattgaggtt gagcgcgtcg gcgagaaacc cgaccaggtt ggggtgcgcg
     3181 gtcagcgcct cgtcaatcgg gcggccctcc gcgttcgccg gatcgaggcc caggatggcg
     3241 ccggcggctg cattcatcaa gaccacgcgc ttgccacgat ccacggccac gatgcccgaa
     3301 cgcaactgcg cgaagacgtg atgcagaacg gttgtgcgat ccatgaaatt ggcccgaccc
     3361 tggagtgtag cagccgtcac gacttcggca aggacaatcg cgccttcggc gggccgtgga
     3421 gggtctcgcc gtgacggcga aggagtcaaa ccagggcgga gggacggata aggcgggggc
     3481 ggccgaacga attgacgacg ccgtatggca gcgggcgtta gttggacgta gtgggtgctt
     3541 gaatggccgc gagcgtcttt ttcgcgtcgc tcgcccacgg cgcggaggga ctgaggcgaa
     3601 gtcccttggt caactcctgc tccgcggcct tgtgatcgcc gttcgcgtac aaggccgttc
     3661 ccagatgata gcgaacggtg gcgttgtccg ggagcttgtc cgagctttcg cgcaacaacg
     3721 cgatggcttt gaggtaggcg ctcttcttgt aatagatcca ccccaaggtg tccgcgaccc
     3781 cggggtcatt cggcaattgc tccatcgcgg tctgcgccaa acccaatgcc acgtcgatgt
     3841 ttttcccttg gtccgcatac agccacgcca ggttgttggc ggcgggcgcg aagcgcgcat
     3901 cgatcttcag ggcgcgttcg tattccgcga tcgcttcgtc cgaccgctgc tgggcctcgt
     3961 aggtcatgcc gagcaggaag tggggcgacg ggttgtccgg ggcggcctcg gccatcgcgc
     4021 ggtactgagc gatgaccttc gcgggctcgc gagtccgggt atagagggtt ccaagggctt
     4081 cataggaggg aagaaaacct ttgttctcat cgatggcccg tttgaactgg gtttcggcgt
     4141 tggtcgcgtc gtccagggcg acatagacgg tgccgagcaa gtggtgaagc ggggcttttt
     4201 gttcggcctt atcgagttgt ccttcgatga gtcggagcgc gccggatcgg tctttgcgct
     4261 gcaacagcat ggccgccact tgttgcacca cgtcgacgga acccggagcc ttgtcgagcg
     4321 cgaggcgaaa ttgcgcttcg gccgcttgcg gtttattcag cgcgcggtac gcgatgcctt
     4381 ttcggtagta cccgacgtag ctggtcggca cgttcgtgat cagcgcgtcg tattgctcca
     4441 tcgcctcgcg gggtttcccg ctcgccagga gcgcgtcgcc ggccatttgc agggccgggg
     4501 ggtatcgcgg caccgcggcc aacaccgcgc gcgcctgttc gagcgccaga tccggggatt
     4561 tggatcgtag gtggagctcc gccaaggcga gacgagcatc ccacgcggtg ggtcggaatt
     4621 tgaccgcctc cgccaattcg gtttgggcct gctgcaggtc gccgcgtctg gcatagagta
     4681 tccccagggc tcggtgggcg tcggcgtggg tcggatctga ggcgaccgcc tgttggaggg
     4741 tggtgacggc gcggtcaacc tgcttgtcca ggatcaagag tcgcgcgtcc agatacagca
     4801 cgtcggcgtc tcctgaccgt tcagccttga ggtcttccac gagcgcacgc gcgtcgtcgg
     4861 gatgctggtg atccagcagg agattcgcga gctgcttttt ggcggccgcg tcgcgcggat
     4921 agtccttgat gaccgcgcgg tactgggtcg ccgcgtcgtc ggctctgttc gcggcgatca
     4981 aatacctcgc gtacgccact cgcgcccctt gttgggtcgg aaacttcgcg accgcctccc
     5041 ggtacgcggc atcggccttg gtctgatcgc cattcagcgc atggaattgg gcgagcacga
     5101 gatacagcgg gaggttatcc ggcttgagcg cgatcgcgga acgatactgg gtttcggcct
     5161 ccgcggtttt cccttgagcg cgataaatgg cggcgaggcc aagcacgggt tccgtgtctt
     5221 ggggggcgag tcgagccgct tccttcacgg agacttccgc ggccgcgtag ttccgcatgc
     5281 gcgcgtagat ggcggcttcg gcgagccagg gtttggattc gccgggatgc gcggttttgg
     5341 ccttggccat ggtcttgagc gcggcatcgg gatgatcggt cgccaattcc acgttggcga
     5401 ggagcatcag cgcgccgagg tgctgcggat ctttggccag gatgaactcg gctttttccc
     5461 gggccttgtc gaaccgttgg cccagcacca ggagttctcc catcttgagg tgcgcgtcca
     5521 ggaactcggg atcgagttcg atggctttgc ccaacgcgat gaaggcctgc tgcgcgtagg
     5581 acgtcgcgtc gtggttcaaa tacgccaccg ccagcctgaa gtgggccttg gcgtcattcg
     5641 ggtcgatttt gagcgcgttg cgaaattcga tcacggcttc ggcatacttc cggtcgtcca
     5701 tgtactgctc ggccttggag aggtgcgcgg ccttttgcgc ggcgggatct ttgctgcaag
     5761 ccgtcccgag cgcgagcgcc aggatcagtg gcaggacgag tgtgacggcg cgtgggacgc
     5821 gaatcattga tcgacggtcg gctggaagac gacgcgtacc ttagcccatg cccgatcaaa
     5881 ggtcaaggaa cggtcaacgt ccgacacggc acggctcaac tttacattcc gaaaaatcga
     5941 ggcgtataat ccgatcgcgt cgcgggatac gtcacgccgt cgcgggcttg ccgcgacaag
     6001 ggacagacag gaagggacaa ggatgaagcg gtcgcgcaat atcgtgttgg cactgattct
     6061 ggtgggagtc ggggtggtcc tgggggtcac gatggcctcg gatctggggt ggttgcccct
     6121 gggtgaagcg cagaacgaaa ccaaggcggc gcgtccggtg ctgaccgggg gcctgccgaa
     6181 cttcgtcgag gtctcgaagg cggtcaagcc ggccgtggtc aacatttcga ccacgcggat
     6241 ggtcaagagc gaagaagggc agggtccggg gaatccattc tttgaagatc cgttctttcg
     6301 ccggtttttc ggcgacgagt tctatcggca attcgatacg ccccgacagc gcaaggagca
     6361 gagtctgggg tccggcgtga tcgtggacgg cgggtacatc gtgaccaata accacgtggt
     6421 cgccaaggcc gacgagatca aggtcgtgat gtccgacaag cgggagttca agggaacgct
     6481 ggtcgggacc gatcccaaga cggatctggc cgtgatcaag gttcccgcga cgggcctgca
     6541 gaccattccg tggggggatt ccacgacgtt ggaggtcggc gagtacgttc tggcggtcgg
     6601 caatccgttc ggactgaacc agaccgtgac catgggcatt gtcagcgcgg tggggcgggc
     6661 caacgtcggc atcgcggatt atgaggactt cattcagacc gacgcggcga tcaatcccgg
     6721 caactccgga ggggcgttgg tcaacgtgaa gggcgaactc gtggggatca acaccgcgat
     6781 cttcacgcgc agcggcggtt atatgggcat cgggttcgcg gttccgagcc agatggtcaa
     6841 gtccgtcatc gaaagcctgg tcagcaaggg caaggtggtc cgcgggtggc tcggtgtcac
     6901 gatccaggaa gtcagccctg agttggccaa acagttcggt ctgaaggaag cccgcggcgc
     6961 gctggtcagc gacattttgg aaggcagccc ggccgataag gccggaatca agcgaggcga
     7021 cgtcgtgatc gaggtcgatc acaagtcggt ggagaacgcg tcccagcttc gcaatttggt
     7081 cgcggcgttg tccgtgggct ccaaggcgag cgtcaaggtg atccgcgaga aaaaagaaaa
     7141 ggtctttgat gtcgtaatcg gcgagcagcc caaagatctc gcgggcaaga gcggagaacc
     7201 gtccgaggaa gacgggggcg agggcgcgct agccgggatc ggcgttcacg atctcagtcc
     7261 ggaattcgcg aaacggttcg gcttgcccga ggacgaggag ggcgtggtgg tcacgcgcgt
     7321 cgagccgggc agtgccgcgg aagacgccgg tgtgcagcgc ggcgacctca ttctggagat
     7381 caatcgccag cccgtccaga caacccgcga gttcagccgg atcgcgcggg agatcggcaa
     7441 ggacgactcg attctgctgc tcgtgaatcg ccagggacaa gcgctgttca tcacgattac
     7501 gccttaggga ggacacggcc tcaatgtgga gcgcgcgaat cgttctggtg ttcgcttcgc
     7561 tcgtgttggg cgccgtgttg gcacggggcg gaggcgccca ggggctgcaa gcgctcatcg
     7621 tcggtctggc gatcggcctt gcgctggccg cggttccctt ggggttggat gcgctcgccg
     7681 cggcgtggtc atggcagcgc gtggcgggct ccgtgctcgg cggggttgcc gggattgccg
     7741 cgtcgggcgt ggtgttatgg gcgacgagtc gggtgtcgct gggcccgacc atggcccggt
     7801 attggcccgt ggcggtgctc gttgcgctgg ggtacgcggg ctggcgcttc ggcgggcagt
     7861 gggccgcgat gttgatgttt gccgcgtctc cgccgagttc cgcgccgcgg atgccgtcga
     7921 atgtggttgc cgggcgcaaa ttgttggaca ccagcgtgat tatcgacggc cgggtcgccg
     7981 acctgtgtga caccggtttt ctggagggta acttgttggt tccgcagttc attttgcagg
     8041 aactccagca tatcgcggac tcttcggata ccctcaaacg cgcgcgcggt cgtcgggggc
     8101 tggatattct ccaccgcatc cagaacatgc cgggcgtgac cgtgaccatt ctcgacgagg
     8161 acgtgccgca ggtgaaggac gtggacgcga aactcgtggc gttggcgaaa aagctggacg
     8221 cgaaaatcgt cacgaatgat ctgaacctca acaaggtcgc cgggctccaa ggcgtgcggg
     8281 tgctcaatac gaacgagctc agcaacgcct tgcggccggt cgtcctgccg ggcgagccga
     8341 tgcgcgtcct ggtccaacgc gagggcaagg agaccggaca aggcgtcgcg tacctggacg
     8401 acgggaccat gatcgtggtg gacgacggca ggaaatacat cgggcgacac gtcgaggtgg
     8461 tggtcacgag cgtgttgcaa accgcggcgg gacgcatgat cttcacccgt ctgcgggaag
     8521 acgcggaccg cggcgaatcg ggcgtccgtc cggaacggtc actgtccgcg tgagcgtggt
     8581 agccgtgatt cccgccgccg gcagcggatc ccggctgggc cgcgggtccc ccaaacaata
     8641 ccttccgctc gccggcatgc ccgtcttggc gcgaaccctg gcggtcttcg acgcgtcgac
     8701 ggcgatcgac gacatcgtgg tcgtggtccc gccgggtgat gaatcgcgat gccgggagac
     8761 cgcggtcgag ccgtacggct ggaaaaagat ccgggccatt gtgccgggcg gcgcggtgcg
     8821 acaagagtcg gtggcgtgcg ggctcgccgc ggtgggcgcg gacgccgcga tcgtggtggt
     8881 gcatgacgcg gcccggcccc tggtcacgga cgatctgatt cgccgggtgg tcgcggcggc
     8941 cgcggcgacc ggcgccgcga tcgcggcgtt gccggtggtg gacacgctga aacggcgcgt
     9001 cggcgagaag gggctcgtga ccacggtgga tcgcgagggg ttgtggacgg cccagacgcc
     9061 gcaggcgttt cgcgtcgggt tgctgcggga cgcggtggcg cgcgcccgcg cggacgggtt
     9121 cgtcgggacc gacgacgcga gcgtggtcga gcggctcggg ttgccagtgg cgctggtgga
     9181 gggcgaggcc tcaaacttca aaatcacgcg cgccgaagac ctcggacgcg cggaagaaat
     9241 cctgctccac gctcgggacc gggcctcgcg tccggccgcg ttggatcgcg tcgggtcccg
     9301 gttctcgtga tgcgcatcgg aatcggctat gacgtgcatg ccctggtcgc tgggcgtccg
     9361 ctggtgctgg gcggggtcac gattccgtcc gagcggggcc ttgacgggca ttccgacgcg
     9421 gatgcgcttc tgcatgcggt ggccgacgcc ctgctcggcg ccgcgtcgct gggggacatt
     9481 ggtcagcact tccccgattc cgatccggcc taccgcaata tctccagtct gcggttgctg
     9541 gaggcggtgg tggagcgcct cgaagagcac ggttttcagg tcggaaacgt ggatgcggtg
     9601 gtgatcgccg agtcgccgaa actggcgccg tatatcccgc ggatgcgcac ggccatggcc
     9661 aaggcgcttc gcgttccgga gacggccgtg agcgtcaagg cgaccaccca cgagcggctc
     9721 ggaagtttgg gccgcggcga aggcatcgcg gcgcaggcgg tgtgcctctt gaacgggaaa
     9781 ggaacgccga ggtgatgtgg cggaccctca agcgtgactt tgccgcggta ttcgaac
//
LOCUS       DBNGTT010000197         4359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2084766, whole genome shotgun sequence.
ACCESSION   DBNGTT010000197 DBNGTT010000000
VERSION     DBNGTT010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2084766"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..795)
                     /locus_tag="AC16BC_04480"
     CDS             complement(154..795)
                     /locus_tag="AC16BC_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3240906.1"
                     /translation="MGKPIRVAVVATSPLLHDGLVLVLREEPTLLVSADMVTCRELAA
                     ETWPIQPDMVVVHVPETPTPAAWREMMMVGLRAKVMMIVRHHDAGLMKRALQLGINGV
                     LTEGVTREAIVNAIHEVMAGAVWCEAGLLAGPHNSAVPMPSKREGEVMALVLEGMSNR
                     DIADRLCISERTVKSHVNRLLQKFHVKNRVQLALHVDEVRPDTTPVTRPPNVQ"
     gene            1312..>4359
                     /locus_tag="AC16BC_04485"
     CDS             1312..>4359
                     /locus_tag="AC16BC_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240907.1"
                     /translation="MSVLLLLGVLGLSSTSFARHGEEGFLTNRIPEPAPYVVEETTSA
                     GGAFGAPHTNAPFESVWLSQNDAGTCDGCHTTLWNQWNGAMMSNAWRDPGWRGAFLLV
                     ARLTSTDGCNDIPAPPDGSQRMDCVTVNPATGQPYKINPFANANGTSTYNMGTSNATY
                     SGSGSLMDDFCSRCHMPSNYVDAVTSVTNDAPSNQEHGRISPLYDPTSLGASQATDPA
                     NGQLTLESFGERTYVDGASRVVNSNTGKAGIFCEVCHTNVATRYTPYHLYNKSGTEYT
                     PAPQNTGRYNQGLTAAQQDMLTVPDAASGNLGYAVAAGAFRLSPHALNMPERFGPLSW
                     NDPCVILPGNCVGGQVLDPYITNVFTGGTRTTNVYFTMANPPGAHDTFYQVKFERAEF
                     CAACHDVTNPMTIKNAAGFWVGGFPIERTYTEWRNSRYSDRPGNPYYDANYKRDCQTC
                     HMEQAYGEPGTALTLYTVSGPTVTPDAPISEPSCDRIVHAPGYSHHFVGGNGYITRLI
                     GADIDGGGTVQPYPELLHTSFSSADANSRYHYARYDTAGTVTGGTTQHERFSWDRMRN
                     VLTMTLTAPGTPVAKGSTAPLTIAVKNEGSGHNFPTGFPEGRAAWLLVKAWDTQNTAS
                     TADDVELMIRDSQKPAGQQDSLGVGYLTAATMTDPTYPQTCPDTEGVATGWKIPEGSI
                     DPWAVTFKAMATVDKVCPTLDLPYATPVNLVVNGNNMPTDAGGVVIDRNNPTGIPRYT
                     DTDPGGNGDVFDDSFLRDTRLQPCRNAGCTNPDNGVSVTRYAVVVPANAVGPIAVTSV
                     VYYQAFEGIVAKKFLGNLANTDDDAPPTLEPCVLKGPCDRASNNTEYRNALKFDPVVV
                     EGAPAIPMEVRNAVIQVSGTTDAVAPTVVINNCKPPKGGGGTCPGTNLTAPIPANGHW
                     SPSPYGGPTGNYATEGYGELSVAQTRIVKVSFSEPVQECDNVPGLVNCGPLSANTYYF
                     TDNRDAAVTFLSDQIDDRTWGIFPFVSGDAIFLSGGGYKIHVAP"
BASE COUNT          814 a         1412 c         1415 g          718 t
ORIGIN      
        1 ccagcggggc tgacctcatg tatggccgac gcgcgaacga cgatgcgcga cggtggcgcg
       61 aggtcagccc cttttttgcg tcgttctgtc gttttacgaa ccggtgagcg ccgccagctc
      121 ggcgtgatgg tcgaagcagc ggaccgggtc gacctactgg acgttcggcg ggcgcgtaac
      181 gggtgtggtg tcgggccgca cctcgtcgac gtggagagcg agttggaccc ggtttttcac
      241 gtgaaacttc tggagcaatc ggttgacgtg gctcttcacc gtccgctcgc tgatgcagag
      301 ccgatccgcg atgtcgcgat tggacatgcc ctcgaggacc aaggccatca cctcgccctc
      361 gcgtttgctc ggcatgggaa cggcgctgtt gtgaggtccg gcaagcagcc ccgcctcgca
      421 ccacacggcc cccgccatga cttcgtggat cgcgttcacg atcgcctcgc gggtcacgcc
      481 ctcggtgagc acgccgttga tgccgagctg cagcgcgcgc ttcatgagcc cggcgtcatg
      541 gtgtcgcacg atcatcatca ctttggcgcg caacccgacc atcatcatct ctcgccacgc
      601 ggcgggcgtg ggcgtttcgg ggacgtggac cacgaccatg tcgggttgga tgggccatgt
      661 ctcagccgcc aactcacggc acgtcaccat gtctgcggat accagaagag tcggttcctc
      721 gcggaggacg aggacgagtc cgtcgtgcaa caagggcgac gtcgccacca cggctactcg
      781 gatcggctta cccataacac gcgctcgccg gtgagaaagc tgaacctgcc gaagacagtg
      841 gaagcgttgt ggcaggaccg tttaccgtac cacgaagaga gccgtcattc tgaggagtga
      901 tgatttctcg caaaatagca caaccgcgag agcgaggtca attcagattt tgtcggcaga
      961 tcgtgcctgc tgccggtgtc ctcctgttgt agcggttctt tccactccgc aaggtcagtc
     1021 gcgtgtcggg ctggtgcgcg tgttgagaaa aactaccacc gcccacttcc actttttccc
     1081 ccggcatctt gcaagaaaat cctaccttcg gacaattgtt cgcggcgggg gtgctccggt
     1141 aaggtgttgg acgttccgta aaagcctgac cggatggcaa gggcacgggc gagctctggg
     1201 cgggttccat caacacgggg tttttggttg ggtaacgacc ggaaacgtga gtgtgacgga
     1261 aagggggcta gcgggcgtat gcagatcgcg gatcagaaat ggcgagtcgg attgtcggta
     1321 ctcctcctgt taggtgtgct tggattatcc tccacgtcgt tcgcgcggca cggggaggaa
     1381 ggatttctca caaatcggat tcccgagccg gcgccgtatg tggtggagga aaccacctcg
     1441 gccggcgggg cgttcggcgc gccgcacacg aacgcgccgt tcgagagcgt ctggctcagt
     1501 caaaacgacg caggtacgtg cgacgggtgc cacaccacgc tgtggaatca gtggaacggc
     1561 gccatgatgt cgaacgcctg gcgcgatccg gggtggcgag gggcgttcct cctggtggcg
     1621 cgtctgacct cgaccgacgg gtgtaacgac atcccggcgc ccccggacgg ttcccagcgc
     1681 atggactgcg tgaccgtcaa tcccgcgacc gggcagccgt acaagatcaa cccgttcgcc
     1741 aacgcgaacg ggacctcgac ctacaacatg ggcacgagca acgcgaccta cagcggctcc
     1801 ggctcgctga tggacgactt ctgctcgcgc tgtcacatgc cgtcgaacta cgtcgacgcg
     1861 gtgacgtcgg tgaccaacga cgccccgtcg aatcaggagc acggacggat cagcccgctc
     1921 tacgatccca cgtcgttggg cgcgtcgcag gcgaccgacc cggccaacgg gcagttgacg
     1981 ctggagtcgt tcggcgagcg gacctacgtg gacggggcca gccgggtggt caacagcaac
     2041 accggcaagg ccggcatctt ctgcgaggtc tgccacacca acgtggccac gcgctacacg
     2101 ccgtatcacc tctataacaa gtcggggacc gagtacaccc cggccccgca aaacaccggg
     2161 cgctacaacc agggcttgac cgcggcgcaa caagacatgc tgaccgtgcc ggacgcggcg
     2221 agcggcaact tgggctacgc ggtggcggcc ggcgcgttcc ggctctcgcc ccacgcgctc
     2281 aacatgccgg agcggttcgg gcccttgtcc tggaacgacc cgtgcgtgat cctgccgggc
     2341 aactgcgtgg gcggccaggt gcttgatccc tatatcacca acgtgttcac gggcggcacg
     2401 cggacgacca acgtgtactt caccatggcc aacccgcccg gcgcgcacga cacgttctat
     2461 caggtcaagt tcgagcgggc ggagttctgc gcggcctgcc acgacgtcac caatcccatg
     2521 accatcaaga acgccgccgg cttctgggtg ggcggattcc cgatcgagcg gacctacacc
     2581 gagtggcgca acagccggta ctcggaccgc ccgggcaatc cgtactatga cgcgaactac
     2641 aagcgcgact gccagacctg ccacatggaa caggcctacg gcgaacccgg gaccgcgctc
     2701 accttgtaca cggtgagcgg acccaccgtg acgccggacg cgccgatcag cgagccgagc
     2761 tgcgaccgga tcgtgcacgc cccgggctat tcgcaccact tcgtgggcgg caacggctac
     2821 atcacccgcc tgatcggggc cgacatcgat ggcggcggca cggtgcagcc gtatccggag
     2881 ttgttgcaca cgagcttctc ctcggcggac gccaacagcc ggtaccacta tgcccggtac
     2941 gacacggccg gaaccgtgac cggcggcacc acccagcacg agcgcttctc gtgggatcgg
     3001 atgcgcaacg tgctgaccat gaccctgacc gcgccgggaa cgcctgtggc caaagggagc
     3061 acggcgccgc tgaccatcgc ggtgaaaaac gagggctcgg gccacaactt cccgaccggg
     3121 tttccggagg gccgggcggc ctggctcttg gtcaaagcct gggacaccca gaacacggcc
     3181 tcgaccgcgg atgacgtgga actgatgatc cgcgacagcc agaaaccggc gggccagcag
     3241 gattccctcg gggtgggata tctgacggcg gcgaccatga ccgatccgac ctacccccag
     3301 acgtgtccgg acaccgaggg ggttgccacg ggttggaaga tccccgaagg ctcgatcgat
     3361 ccgtgggccg tgacgtttaa agccatggcc acggtggaca aggtctgccc gacgctggac
     3421 ctgccgtacg ccacgccggt gaacctggtc gtgaacggca acaacatgcc gacggatgcc
     3481 ggcggagtcg tgatcgaccg gaataatccc accggcatcc cgcggtacac ggataccgac
     3541 cccgggggca acggcgacgt gttcgacgac tccttcctcc gcgacacgcg gctgcaaccg
     3601 tgtcgcaacg cggggtgcac gaacccggat aacggggtga gcgtcacccg ctatgcggtg
     3661 gtcgttccgg cgaacgcggt cgggccgatc gcggtgacct cggtcgtcta ctaccaggcc
     3721 ttcgagggca ttgtggccaa gaagttcctg gggaacctgg ccaacaccga cgacgacgcg
     3781 cctcccacct tggagccctg cgtcttgaaa ggaccctgcg accgagcctc gaacaacacg
     3841 gagtatcgga acgcattgaa gttcgatccg gtcgtagtcg agggcgcgcc ggcgatcccc
     3901 atggaggtca ggaacgccgt cattcaggtg tcgggcacga ccgacgccgt ggcgcccacc
     3961 gtggtcatca ataactgcaa gccgcccaag ggcggcggcg ggacctgccc cggcacgaac
     4021 ctgacggccc cgattccggc gaacgggcac tggtcgccgt cgccgtacgg cgggcccacc
     4081 gggaactacg ccacggaggg ctacggcgag ctgagcgtgg cgcagaccag gattgtgaag
     4141 gtctcgttca gcgagccggt gcaggagtgc gacaatgttc cgggactggt caactgcggc
     4201 ccgttgtcgg ccaataccta ttacttcacc gacaaccgcg acgcggccgt caccttcctg
     4261 tcggatcaga tcgacgaccg cacgtggggg atctttccct ttgtaagcgg cgacgcgatc
     4321 ttcctgagcg gcggcggtta caagattcac gtggcgccg
//
LOCUS       DBNGTT010000198         4344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_167610, whole genome shotgun sequence.
ACCESSION   DBNGTT010000198 DBNGTT010000000
VERSION     DBNGTT010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_167610"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1695
                     /gene="pyk"
                     /locus_tag="AC16BC_04490"
     CDS             307..1695
                     /gene="pyk"
                     /locus_tag="AC16BC_04490"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3240908.1"
                     /translation="MRRTKIVCTIGPASDHPDILRAMMHAGMDGARVNFSHGSGREQS
                     ERIARIRAVAESIGRTIAVIGDLAGPKLRTGALSRESVVLRAGDPFILTVKPVPGDWR
                     AVSIASPDLTNAVKPGDTILLSDGTIELTVEHVGDGDVATRVRVGGLLGAHKGINVPG
                     RSLAIDAFTTKDREDLTAAVEQRLDWIALSFVRHVEDVRTARAWMDRAGHRIPVIAKI
                     EQREAIAAISAISDESDGLMVARGDLGVETALEDVPFYQKTVIANALSRGIPVITATQ
                     MLESMVDAPRPTRAEATDVATAVLDGTDALMLSEETATGKYPVDAVRTMARIAERADR
                     EIDEARFLRREADTPAEAVSRAACALAAEIRASAILVPADDGTEPNQVSAYRPRQPVL
                     APTGDPRVAARLAITWGVVPVIHAADRRTPWFRRGVDHAKASGILLSGDCYVAVRPPD
                     REQAGAIEVAYA"
     gene            complement(1686..2477)
                     /locus_tag="AC16BC_04495"
     CDS             complement(1686..2477)
                     /locus_tag="AC16BC_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR3240909.1"
                     /translation="MAIVTLTTDFGLRDPWVGGVKGVLLSIAPDAVLVDLTHEIPRHA
                     VSAAAYVLAATSGYFPVGTVHLVVVDPGVGGARRALACEAGGARFVGPDNGVFGRVLR
                     RHPDARCVEITDPRVMLKPPGESPTFQGRDVFAPAAAWLARGMAIANLGPAINDPTQL
                     PVSEASRNGTDWTGEVVWVDRFGNLISNIEVPRGVSVDAVRVGETDLRVVTSYQAVVP
                     GTAAALVNSDGLIEMFVNRGDASLALKASIGTRVTAGMSPARGQA"
     gene            complement(2479..3417)
                     /locus_tag="AC16BC_04500"
     CDS             complement(2479..3417)
                     /locus_tag="AC16BC_04500"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR3240910.1"
                     /translation="MSAQSPSLTVDLGDGLVLKNPIIAASGTFGYGEEYAGVLDVNRL
                     GAIVVKGLSVEPKTGNPTPRLIETPSGLLNAIGLANVGLEAFLSDKLPFLRRFDVKVI
                     VNIFGGTIEEFERLTDRLSDAQGVHALEVNVSCPNVKQGGMNFGQSVTGVFEVVRAVR
                     RKTRLPMIVKLSPNVTDIAEIAKAAHAAGASALSVINTVLGMAIDVETRRPKLASWTG
                     GLSGPAIRPIAVRMVWEVSRAVPIPIIGLGGVTCAEDAVEFLLAGASAVAVGTANFMN
                     PRATIDVLDGLTRYMARHNVHDVRQLVGAIDVTGRG"
     gene            complement(3488..3694)
                     /locus_tag="AC16BC_04505"
     CDS             complement(3488..3694)
                     /locus_tag="AC16BC_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240911.1"
                     /translation="MDWPTLRLGLHLALGSRRFGVNGRAREPFDSLMTRPLFGFGWGL
                     AVRPWAWRAAWRVIRLRMDIAGWT"
     gene            complement(3704..4180)
                     /gene="greA"
                     /locus_tag="AC16BC_04510"
     CDS             complement(3704..4180)
                     /gene="greA"
                     /locus_tag="AC16BC_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR3240912.1"
                     /translation="MAKIPMTPRGYQALKDELDRLKRVERPKNIQDIAEARAHGDLSE
                     NAEYAAAKERQSFIEGRMRELETQIADAEVIDTSNMTPDKVVFGATVVLEDTDSGDKK
                     QFMIVGQHEIDLKNGRISVHSPVGKALIGHRVGDIVTIVAPAKTTEYQVLEIRFDA"
BASE COUNT          739 a         1495 c         1381 g          729 t
ORIGIN      
        1 ccggtacatg acagatacct tgtgcgtcgg aatcgccgcc gggtattccc tcgacgaacc
       61 tcctcgccgc gagccgcccg caagcgggta ttgccattga aggcctgtca gaccatacta
      121 tgaaccaagg ctcgtgggta ccagactcca aggaacgagg ttgaactcga cgggggctcg
      181 gagcccagca acacgggaag actatttcaa gcgttcaagc ggcgctgtcc ctgttcggtc
      241 gttgattatg tagtccacgg ccgcggcgga ggctgaacac cgcgggactc cagggaaggg
      301 agtgtcatgc gtcgcaccaa aatcgtctgt accatcggcc cggcaagcga ccatcccgac
      361 atccttcgcg ccatgatgca cgccgggatg gacggggctc gtgtcaattt ctcgcacggc
      421 agcggccggg aacagtccga acggatcgcg cgtatccgag ctgtggccga atccatcggc
      481 cgcaccattg cggtcatcgg agacctcgcc ggccccaagc ttcgcactgg agcgttgagc
      541 cgcgagagcg tcgtgttgcg cgccggagac ccgttcattc tgacggtcaa gcctgttcca
      601 ggggattggc gggccgtgtc cattgcttct ccggatctca ccaacgccgt caaacccggg
      661 gataccattc tcttgagcga tgggacaatc gagctcaccg tggaacacgt tggggacggc
      721 gatgtcgcga cacgtgtacg cgtgggcggc ctcttagggg cccacaaagg gatcaacgtt
      781 ccggggcgca gcctcgccat cgacgcgttc accaccaagg accgcgaaga cctgaccgcc
      841 gcggtggaac aacggctgga ctggatcgcc ctgtcgttcg tccggcacgt ggaggacgtg
      901 cgcaccgcca gagcctggat ggaccgagcg ggacatcgca tccccgtgat cgcgaagatc
      961 gaacaacgcg aggcgatcgc cgccatctcc gccatctcgg atgagtcgga cggcttgatg
     1021 gtggcccgcg gtgacctcgg tgtggaaacg gccttggagg acgtgccgtt ctatcagaag
     1081 acggtgatcg cgaacgcgct gagccgcggc attccggtga tcaccgcgac ccagatgctc
     1141 gagtccatgg tggatgcccc gaggcccacg cgggccgagg cgaccgatgt cgccactgcg
     1201 gtgctggacg gcaccgatgc gttgatgctg tcggaagaaa ccgcgaccgg aaagtatccg
     1261 gtggacgcgg tccggaccat ggcgcgcatc gccgaacgcg cggaccgcga gatcgacgag
     1321 gcgcgattcc ttcggcgaga ggcggatacg ccggccgagg cggtgtcgcg ggcggcctgc
     1381 gcgctcgccg cggagattcg ggccagcgcg atcctggttc ccgccgacga cggcaccgaa
     1441 ccgaatcagg tgtcggcgta ccgtccccgg cagccggtcc ttgcgccaac cggagacccg
     1501 cgcgtggcgg cccgcctcgc gatcacctgg ggcgtggtgc cggtgatcca cgccgcggac
     1561 cggcgaaccc cgtggtttcg ccgaggcgtc gatcacgcga aagcctcggg gatcctcctc
     1621 tccggagact gctacgtggc ggtgcgtccg cccgatcgag agcaagccgg cgcgatcgag
     1681 gtggcttacg cctgaccgcg cgccggcgac atgccggccg tgacccgcgt tccgatcgac
     1741 gccttcaacg ccagggacgc gtcaccgcga ttcacgaaca tttcgatcag cccgtcgctg
     1801 tttacgagcg ccgcggcggt tcccggaaca acggcctgat acgacgtcac cacccggaga
     1861 tccgtctccc ccacccgcac cgcatccacc gagacgccgc gcggcacttc gatattgctg
     1921 atcagattcc cgaaccgatc gacccacacc acttccccgg tccaatctgt cccgttgcgg
     1981 ctcgcctcag aaaccgggag ttgggtcggg tcgttgatcg cggggccgag attcgcgatc
     2041 gccatccccc gagccagcca ggccgcggcc ggggcgaaca cgtcgcgtcc ctggaacgtc
     2101 gggctctcgc ccggtggttt cagcatgacg cgtggatcgg tgatctccac gcatcgcgcg
     2161 tcgggatggc gccgcagcac ccgaccgaac acgccgttgt cgggcccgac gaacctcgct
     2221 cccccggcct cgcacgccaa ggcccgcctc gccccgccca ccccggggtc caccacgacc
     2281 agatgcacgg ttccgaccgg gaagtagccg gacgtcgcgg ccaggacgta cgcggcggcg
     2341 ctcacggcgt gtcgcggtat ctcatgggtg aggtccacca gcaccgcgtc gggtgcgatg
     2401 gagagcagca cgcctttgac gccgcccacc cacggatccc ggagcccgaa gtccgtggtc
     2461 agcgtgacga tcgccacgtc acccgcgtcc ggtgacgtcg atcgcgccga cgagctgccg
     2521 cacgtcgtga acgttgtgcc gggccatgta ccgggtcagg ccgtcgagga cgtcgatggt
     2581 ggcgcgcggg ttcatgaagt tcgcggtgcc cacggccacc gccgacgcgc cggccagcag
     2641 aaactccacc gcgtcctccg cgcacgtcac tcccccgagc ccgatgatcg gaatcggcac
     2701 cgccctggac acctcccaca ccatgcgcac cgcgatcggc cggatggcgg gcccggagag
     2761 ccccccggtc cacgacgcca acttcgggcg cctggtttcc acgtcgatcg ccatgcccag
     2821 cacggtgttg atcaccgaca gcgcgctcgc gcccgccgcg tgcgcggctt tggcgatctc
     2881 cgcgatgtcg gtgacgttcg gggacaattt cacgatcatg ggaaggcggg tttttcggcg
     2941 caccgcgcgc accacctcga acacgcccgt caccgactgc ccgaaattca tgccgccttg
     3001 tttgacgttc gggcacgaga cattgacctc cagggcgtgc acgccctggg catccgacag
     3061 ccgatccgtc aatcgttcga attcctcgat ggttccgccg aaaatgttca cgatgacctt
     3121 gacgtcgaat cgccgcagga acgggagctt gtccgacaaa aacgcttcga gccccacgtt
     3181 ggcgaggccg atggcgttga gcaagcccga cggggtttcg atgagacgcg gcgtgggatt
     3241 gccggtcttg ggctccacgg acaggccttt gaccacgatc gcgcccagcc ggttcacatc
     3301 caggacgccg gcgtactcct cgccgtaccc gaacgtcccc gacgcggcaa tgatcggatt
     3361 cttcagcacg aggccgtcac cgagatcgac ggtcaggctg ggagattgcg cgctcatcgc
     3421 taccgggtga tcgcgaccac gttgctacgt tccgatacag gagaagccga cgaccgcgag
     3481 tcgggcctta cgtccatccc gcgatatcca tccgcagacg gatgacccgc cacgcggcgc
     3541 gccacgccca cggtcgaacg gccaggcccc atccgaagcc gaacaacggc ctggtcatga
     3601 gggaatcgaa tggttcccgc gcgcggccgt tcaccccgaa ccggcgggag ccgagcgcaa
     3661 gatggagtcc gagacgcagc gtcggccagt ccacggcgga acgtcaggcg tcgaaccgga
     3721 tctcgagcac ctgatactcg gtcgtcttgg cgggcgccac gatcgtgacg atgtctccca
     3781 cccggtgtcc gatgagcgcc ttccccaccg gtgagtgcac ggagatgcgt ccgttcttca
     3841 aatcgatctc gtgttgcccc acgatcatga attgtttctt gtcgcccgag tcggtgtcct
     3901 cgagcaccac ggtcgcgccg aacacgacct tgtccggagt catgttggac gtgtcgatca
     3961 cctcggcatc cgcgatctgc gtctccagtt cccgcatccg cccttcgatg aacgattgcc
     4021 gctccttggc cgcggcgtat tccgcgtttt cgctcaggtc gccgtgcgcc ctcgcctccg
     4081 cgatgtcttg gatatttttc gggcgttcga cgcgtttgag ccgatcgagt tcgtccttga
     4141 gcgcctggta cccgcgcggg gtcatcggta ttttcgccat ggtcgtttcc tactttgatc
     4201 gtgagccgag gtcctgcgtt tcccggtaat actcctgcac cgaccggatt tcaaccgcgc
     4261 cgcgcggacg ggcctgcatc gcggccaccg cggccttggc cccggcaagg gtcgtaatgt
     4321 atgggatgcc ggacaacaat gtgg
//
LOCUS       DBNGTT010000199         3018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1573021, whole genome shotgun sequence.
ACCESSION   DBNGTT010000199 DBNGTT010000000
VERSION     DBNGTT010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1573021"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="AC16BC_04515"
     CDS             <1..1835
                     /locus_tag="AC16BC_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HIR3240913.1"
                     /translation="VLWSGKPFFERGWASLVSRNFNMFTLIAVGVGAAYVYSAAATIA
                     PGLFPEGFRVHGAVEPYFDSAVVITILVLLGQVLELRARGRTGAAIRALLGLAPKTAR
                     LVRDGREEDIPLDRVQRDDLLRVRPGEKIPVDGIVIEGRTSVDESMITGEPIPVEKTA
                     GAAVVGGTVNGTGSLVMKAQRVGAETLLAQIVRMVGDAQRSRAPIQRVADTVSGYFVP
                     LVMAVSVLAFIAWWLWGPEPRLALALVNAVAVLIIACPCALGLATPMAVMVGVGRGAE
                     TGVLIKHAEALEILERVDTLVMDKTGTLTEGKPTLTNIETAPGFTGDQILRVAASLER
                     ASEHPLAAAIVAAAKARQLGLADAKDFHSVTGQGVSGVVDGSRVALGTQSFLESQGVV
                     LGDLAAKADNKRREGATVVWLAMDQRPAGLFSVSDPIKASTPEAIALLHRAGLRVVMV
                     TGDSRVTAQAVASKLGLDDVRAEVLPAQKRDIVKALQDDGRIVAMAGDGINDAPALAQ
                     AHVGIAMGAGTDVAMQSAGVTLVKGDLRAIARARGLSRGTMRTIRQNLFFAFVYNVIG
                     VPVAAGVLVPFGGPLISPIWASAAMSFSSVSVIANSLRLRHSSL"
     gene            2064..2927
                     /locus_tag="AC16BC_04520"
     CDS             2064..2927
                     /locus_tag="AC16BC_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3240914.1"
                     /translation="MTDDDWVFNADEANFEERVIARSQTAPVVVDFWAEWCGPCRYLG
                     PILEDAVEDFEGKILMAKVDTDRNFNLAQRFRIQTIPNVKAIFKGKVIDEFVGALPET
                     AIKAFLQRLIPTKADELTDQGAALERGRQWDDALRAYREALGQDARHPVAAVGELRVL
                     VQLARWDDARTAYDRLPGPLQVQPEVTALKARMDLAAGSAGGPSIADLETIAKNNPGD
                     LDARFQLAARYAAEQRYRDALDAYVAILEKDRRFRDAAPRKMMLQIFEVIGMRSPLAE
                     EYREKLARLIY"
BASE COUNT          484 a          982 c         1055 g          497 t
ORIGIN      
        1 tggtgctctg gtccggcaaa ccgttcttcg aacgcggatg ggcttcattg gtgagccgca
       61 acttcaacat gttcacgctg atcgcggtgg gcgtgggcgc ggcgtacgtc tacagcgcgg
      121 ccgccaccat cgcgcccggc ctgttccccg aggggtttcg cgtgcacggc gcggtggagc
      181 cgtacttcga cagcgcggtg gtgatcacga tcctggtcct gctgggccag gtgctggagc
      241 ttcgcgcgcg gggccgcacg ggcgcggcga tccgcgccct gctgggcctc gcgcccaaaa
      301 ccgcgcgcct ggtccgcgac ggccgcgaag aagacatccc gctcgaccgc gtccagcgcg
      361 acgacctcct ccgggtgcgc ccgggtgaaa aaatcccggt ggacggaatc gtcatcgagg
      421 gccgcacctc ggttgatgag tcgatgatca cgggcgagcc cattccggtg gagaagacgg
      481 cgggagcggc cgtggtcggc ggaaccgtga acgggaccgg ctcgttggtc atgaaagccc
      541 agcgggtcgg cgccgagacc ctgttggcgc agatcgtgcg catggtgggc gacgctcaac
      601 gcagccgcgc gccgatccag cgcgtggccg acaccgtgtc cgggtacttt gtgccgctcg
      661 tgatggcggt cagcgtgctc gcgttcatcg cctggtggct gtggggtccg gagccccgcc
      721 tggccttggc cctggtcaac gccgtggccg tgctgatcat cgcgtgcccg tgcgcgctcg
      781 gcctggccac gcccatggcc gtgatggtcg gcgtgggacg cggcgcggaa accggcgtgc
      841 tcatcaaaca cgcggaagcg ttggaaatcc tcgaacgcgt ggacaccttg gtgatggaca
      901 agaccggcac gttgaccgag ggcaagccca cgctcaccaa catcgagacc gcgccggggt
      961 tcaccggcga tcagatcctg cgcgtcgccg ccagcctgga gcgcgccagc gagcacccgc
     1021 tcgcggccgc catcgtggcc gcggccaagg cccggcaact gggcctggcg gacgcgaaag
     1081 atttccactc cgtgaccggc cagggcgtca gcggcgtggt ggacggctcg cgcgtggcgc
     1141 tcggcacgca gagcttcctg gagtcccagg gcgtggtgtt gggcgacctc gcggccaagg
     1201 ccgacaacaa acgacgcgag ggcgccaccg tggtctggct cgccatggat caacggcccg
     1261 cggggttgtt ttcggtgtcc gacccgatca aagcgtccac gccggaagcc atcgcgctcc
     1321 tgcaccgcgc cggactgcgc gtggtgatgg tcacgggcga ctcgcgcgtc accgcgcaag
     1381 ccgtggcgtc gaagctgggg ctcgacgacg tgcgcgccga ggtcctgccc gcgcagaagc
     1441 gcgacatcgt caaggccttg caggacgacg gacggatcgt ggccatggcc ggcgacggga
     1501 tcaacgatgc gcccgccctg gcccaggcgc acgtgggcat tgccatgggc gcgggcacgg
     1561 atgtggccat gcagagcgcg ggcgtcaccc tggtcaaagg cgacctgcgc gcgatcgcgc
     1621 gggcgcgcgg cttgagccgc ggcaccatgc gcacgatccg acagaacctc ttcttcgcgt
     1681 tcgtgtacaa cgtgatcggc gtgcccgtgg ccgccggagt gctggtgccg ttcggggggc
     1741 cgctcatcag cccgatctgg gcgagcgcgg cgatgagttt tagctcggtg tcggtgattg
     1801 ccaactcgct ccgtctccgc cactcctcgc tgtagagcct gacgatgaac ggtcatccgc
     1861 ggcgttgtcg ctgcgcgtcc gctcctcacg tacgacccag tacgctacgg tgcgggcgct
     1921 cgctccgcct tgcgtctgac gcgtccctcg tcaggctccg ctgtcgcggg aacttcgagc
     1981 caccgattta gtgacagcgc cgctttctga tgcgtatttc ctgcttgccg ctcgtcactg
     2041 aactgattta cactgctgcc cttatgaccg acgacgattg ggtgtttaac gccgatgagg
     2101 cgaactttga ggagcgcgtc attgcgcgct cgcaaaccgc gccggtggtg gtggattttt
     2161 gggcggagtg gtgcgggccg tgccggtatc tgggaccgat cctcgaagac gcggtcgagg
     2221 atttcgaggg caagatcctc atggccaagg tggatacgga ccgcaatttc aacctcgcgc
     2281 agcggttccg catccagacc attccgaacg tgaaggccat cttcaagggc aaggtgatcg
     2341 acgagttcgt gggcgcgctc cccgagaccg cgatcaaagc attcctccag cggttgatcc
     2401 cgaccaaggc ggatgagttg accgaccagg gcgcggcgct ggagcgcggc cggcaatggg
     2461 acgatgcgtt gcgcgcgtat cgcgaggccc tcggccagga cgcgcgccac ccggtggcgg
     2521 cggtgggcga gcttcgcgtg ctggtgcagc tcgcgcgctg ggacgacgcg cggaccgcgt
     2581 acgatcggct cccgggcccg ctgcaagtgc agcccgaggt caccgcgctc aaagcccgga
     2641 tggacttggc cgcggggagc gcgggaggac cgtccatcgc ggatctggaa acgatcgcga
     2701 agaacaatcc gggtgacctg gacgcgcggt tccaactggc cgcccggtac gcggcggaac
     2761 agcggtatcg cgacgcgttg gacgcgtacg tggcgattct cgaaaaagac cgccgcttca
     2821 gggacgccgc gccgcgcaag atgatgcttc agattttcga ggtgatcggg atgcgaagtc
     2881 cgctcgcgga agagtaccgc gaaaagctcg cgcgactgat ttactagcga ccatcagtcc
     2941 atgagtcccc aacgcaaaag caaatccccc ctcacccccc atttccaaag gggggaagtt
     3001 catacgttgt ggtcgaac
//
LOCUS       DBNGTT010000200         3715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_40812, whole genome shotgun sequence.
ACCESSION   DBNGTT010000200 DBNGTT010000000
VERSION     DBNGTT010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_40812"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1434
                     /locus_tag="AC16BC_04525"
     CDS             202..1434
                     /locus_tag="AC16BC_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3240915.1"
                     /translation="MARYRYKARDKSGSVMTGVIESSGRDAAAIHLNQLGYFPVRIDE
                     TVGRDWRELFARAEEWFAQVTPQDKIIMTRQMATLIDAGLSFVAVFDALIEQTMNQKL
                     RGILIQVRRDVEGGLPFSNALAKHPAVFEELYVNMVRAGEEAGALDEMLDRLAFLAER
                     DADTRARINAATRYPVIVMVALAVAFGILVTFVIPQFSKLYANAHAALPLPTRMMIGL
                     NSLVQSYGAILLGAVIAGIVGLRAYVNRPTGRVWWDTLKLRIPVFGPLFLKSALSRFA
                     RTFGTLNRCGFPALQALDIVAKTVGNVAVSRGIELIQDGARQGRGLVQPMKASRLFPA
                     GVVQMVAIGEETGQLEAMLMKVSEYYDREVDYSIKNLSSALEPFLLVVIGAAVLFLAL
                     AIFLPWWNLIHVFKGGGG"
     gene            1487..1834
                     /locus_tag="AC16BC_04530"
     CDS             1487..1834
                     /locus_tag="AC16BC_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240916.1"
                     /translation="MVCIVAAGLVLVVMAYFDRLTREARETALRMGLGNIRMGVRLYH
                     ALNERYPHDIRELFATGYLLPAGKDSVFVARYLEDQTLDHDGYPVDPFGHRYRYDPVG
                     GRVLSESKGYEQW"
     gene            1929..2363
                     /locus_tag="AC16BC_04535"
     CDS             1929..2363
                     /locus_tag="AC16BC_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3240917.1"
                     /translation="MKRLKNEAGFTMIELVVVIVILGILAAFAVPQFANLQTEARVAA
                     VNGMAGAIRGAVSIARAKYLVVGNMAATTVDMDGTAVDVIAGTGRPAGSATGIQAALQ
                     DTSGFTIVTGATTTFTPINGGSGTCKVDYVAATGAVTAFPTC"
     gene            2384..2968
                     /locus_tag="AC16BC_04540"
     CDS             2384..2968
                     /locus_tag="AC16BC_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3240918.1"
                     /translation="MRGACEKGSLAPRYFSGCLERRKRRYFVVKERERGFTLIEAVII
                     IVLVAVVAILVVPRLPNLVGTKASGTARKLQSDLAYVQSLSMTTNTRHRLVFLSTTTY
                     EVRDATGTLVANPDGEGGFSIATGSGITLSWNLNGVTVANRGVEFDSLGRPYLYAGST
                     PSTTNLATGTITVTGGGTTQTVTVRAQTGMVSIP"
     gene            2985..3446
                     /locus_tag="AC16BC_04545"
     CDS             2985..3446
                     /locus_tag="AC16BC_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR3240919.1"
                     /translation="MRKRAATNEQGVTLIETVIVIIVLGLSLAVLIPSTVSTQHSATP
                     VLTQQAAALAQEKLEQIFADRLDQTTPRGFTYATTPGNYPAENPIGGVTGFSRSVAIT
                     CVTTANLNAAGSAPSPSCAASAGRTDYARVTVTVTNGAIGSVTAATLLTNY"
     gene            3446..>3715
                     /locus_tag="AC16BC_04550"
     CDS             3446..>3715
                     /locus_tag="AC16BC_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3240920.1"
                     /translation="MATLRRHSRPRIQDQSGVTLIELVITIVIVGIIAATTSLLLLTG
                     VREYSAQDARVTITTQGRLSLERMAREIRLIRSRTAADIPTMTATT"
BASE COUNT          706 a         1049 c         1214 g          746 t
ORIGIN      
        1 ccgcgagcgg catcacgacg ctcgaagaag tttatcgcgt cacccaagac gcggatgccg
       61 acgtggggtg acgatttctg gtctcactgt gaaatgtgtt ccacggccac gtgggtcatg
      121 taaccaaaat tttgaagaga accgtttctt cagataacac aggaagatcc gatggcgcat
      181 cgattgccct gaggcttaca gatggcgcgg tatcgctaca aagcccgtga taaatcaggg
      241 agcgtgatga ccggggtcat cgagtcctcc gggcgcgacg ccgccgcgat ccacctcaac
      301 caactcggct atttcccggt tcgcatcgac gagacggttg ggagggattg gcgggagttg
      361 ttcgcccgtg cggaggagtg gtttgcccag gtcacgccgc aggacaagat catcatgacc
      421 cggcagatgg ccacgttgat cgacgcaggc ctttcgtttg tggcggtgtt tgacgcgctc
      481 atcgagcaga cgatgaatca gaagctgcgc ggcattctca tccaggtccg gcgcgacgtc
      541 gagggggggc tgccgttttc gaacgcactt gcgaagcacc cggccgtgtt cgaggaactc
      601 tacgtcaaca tggttcgggc cggggaagaa gcgggcgcgc tggacgagat gttggaccgg
      661 ctggccttcc ttgccgagcg cgatgcggac acccgggcgc ggatcaacgc ggcgaccaga
      721 tatcccgtga tcgtgatggt ggcgctggcc gtggccttcg gcattctggt gaccttcgtg
      781 attccgcaat tctccaagtt atacgccaac gcccacgcgg ctttgccctt gcccacgcgg
      841 atgatgatcg ggctcaactc gctcgttcag tcgtacggag cgattctatt gggggccgtg
      901 atcgccggca ttgttggtct gcgcgcctac gtcaaccggc ccacgggccg cgtgtggtgg
      961 gatacgttga aacttcgcat ccccgtcttc ggcccgttgt ttctcaaatc cgcgttgtct
     1021 cggttcgccc ggacgttcgg cacgttgaat cgatgtggat tcccggcctt gcaggcgctc
     1081 gatatagtgg caaagacggt cggcaatgtc gccgtgtctc gtgggatcga gctgatccag
     1141 gatggcgcgc gtcagggtcg aggcctggtc cagccgatga aagccagccg attgttcccg
     1201 gccggcgtcg tacagatggt ggcgatcggc gaagagacgg ggcagcttga agccatgctg
     1261 atgaaagtct ccgaatatta cgaccgcgaa gtggactact cgatcaagaa cctgtcgtcc
     1321 gcgctcgaac cgttcttgct cgtggtcatc ggcgccgcgg tgttgtttct ggcgctggcg
     1381 attttcctcc cgtggtggaa tctgatccat gtgtttaagg gggggggagg ttgatcgcgc
     1441 ggtgcgcgtg ccaccaaccg agagacggag ggttcttgat gctctgttgg tctgcatcgt
     1501 ggccgcgggg ctggtgctgg tggtgatggc ctatttcgac cgcctcacac gtgaggccag
     1561 agaaaccgcg ttacggatgg gtttggggaa catcagaatg ggcgtccgtc tataccatgc
     1621 gctcaatgaa cgctatccac atgacatcag ggaattgttt gcgaccgggt atttgctgcc
     1681 cgccggaaag gattcggtgt ttgtggcgcg ctacctggag gatcaaacgc tggatcacga
     1741 cggatacccg gttgacccgt tcgggcaccg ctatcggtac gatccagtcg gagggcgcgt
     1801 cttgtcagaa tcgaaaggat atgaacaatg gtaggggaac gcgggtgcgt cgggagccgg
     1861 agaggcttgg cagtgtcgcg tgtcaacatg ggcagcaggg cagtgaaccc acacatggga
     1921 ggaggggtat gaagcgactc aagaacgagg cgggttttac catgatcgaa ctggtggtcg
     1981 tgattgtgat cctcgggatc ctggcggcgt ttgcggttcc gcaattcgcg aacttgcaga
     2041 ccgaagcccg cgtggccgcg gtcaacggga tggcgggtgc gattcgcggt gcggtgtcaa
     2101 tcgcgcgcgc aaaatacctg gtggtcggca atatggccgc aactacggtt gacatggacg
     2161 gcaccgccgt ggacgtaatc gctggcacag ggcggccggc cggttcggcg actggtatcc
     2221 aggccgcgct tcaggacacg agcggcttta ccatcgtaac tggtgcgacc acaaccttta
     2281 caccgattaa cggcggtagt gggacctgta aggtggacta tgttgctgcc accggcgcgg
     2341 tcacggcgtt tcctacctgt taacacctgc ggagcagatg atcgtgcgag gggcatgcga
     2401 gaaaggctcg cttgcccctc gctatttttc agggtgtctc gaaagaagga agcgacggta
     2461 tttcgtggtc aaggaacgcg agcgcggttt tacattgatc gaagccgtga tcatcatcgt
     2521 cttggtcgcg gtggtcgcga ttcttgtggt cccacggctg ccaaatctgg tcgggacgaa
     2581 ggcgtcgggc acagcgagga agctacaatc ggatctcgcg tatgttcaaa gcttgtccat
     2641 gacgaccaac acccggcatc ggctggtctt cctgtcaacg accacgtatg aagtcaggga
     2701 tgcgaccggg accctggttg ccaatccgga tggggagggc gggttttcta tcgctaccgg
     2761 gtccggcatc acgctaagtt ggaatctcaa tggcgtcacg gtggcgaacc gcggagtcga
     2821 gttcgactcg ctcgggcggc cctatctcta tgccggttcg actccgtcga cgacgaacct
     2881 tgcgaccggg accatcaccg tgactggggg ggggacaacg cagaccgtca ctgtccgcgc
     2941 ccagaccggg atggtgagta ttccgtagag gtcggccgat gacggtgagg aaacgagcag
     3001 ccaccaacga gcaaggggtc acgctcatag aaacggtgat cgtgatcatc gtgttggggc
     3061 tctcgctggc cgttctgatc ccatcgaccg tgagcactca gcacagcgcg acgcccgtgc
     3121 tcacgcagca ggccgcggcg ctggcccaag aaaaactcga gcaaattttc gcggatcgtc
     3181 tggaccagac cacgccccga gggttcacgt acgcgaccac gccgggaaac tacccggcgg
     3241 agaatcccat cggcggggtg acgggctttt cccggtcggt ggcgatcacg tgcgtcacca
     3301 ccgcgaatct caatgccgcg ggatcagcgc ccagtccgtc gtgcgccgca agcgccgggc
     3361 gcacggacta tgcccgcgtc accgtgaccg tcacgaacgg agcgatcggc agtgtcactg
     3421 cagcgaccct gctgaccaat tactgatggc gaccctgagg cgtcacagtc gtccccgcat
     3481 tcaggaccaa tccggcgtca cgctcattga attggtcatc acgatcgtga tcgtgggcat
     3541 catcgcggcc accacttcac tgctgttgct caccggagtc cgagagtact cggcgcagga
     3601 cgctcgggtg acgatcacga ctcaggggcg gttgagtctt gaacgtatgg cgcgcgagat
     3661 tcgtctgatc cggagccgca ccgcggccga cattccgacc atgaccgcga ccacg
//
LOCUS       DBNGTT010000201         1782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1829349, whole genome shotgun sequence.
ACCESSION   DBNGTT010000201 DBNGTT010000000
VERSION     DBNGTT010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1829349"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="AC16BC_04555"
     CDS             <1..290
                     /locus_tag="AC16BC_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240921.1"
                     /translation="ERVFAATAAKGGRTGYVAALAQAGEGLFASVNGRVWRSRDGHAW
                     REVGQLTPFTVEAMHEFGGALYAGTTMPPRAWVYRSRVSDLPSPDDRPAPR"
     gene            343..1737
                     /locus_tag="AC16BC_04560"
     CDS             343..1737
                     /locus_tag="AC16BC_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3240922.1"
                     /translation="MRTHVSSPAGPSSPRLSGLAPARVWGAALVAACLAGCARAPSPP
                     PATSAPVSPPAIFVSQIALHPARPDRVLALTNYSVGLLASRDRGAHWATANRGIRSYS
                     LYRLVVDPRDPDLVYVGAGGGGLYQSVDGAKTFVERNNGLGNTNVGAIVLHPVDPDRV
                     FVVTSTGVFISPDRGATWAAWNQGDDFTQSQQFQDLVILPGELETVLLASNRGVWVRR
                     AGDAAWAPASGDLQGVAITVLIRHPDGRRVFAAALRNGKTLEGGGLYVNDTSGAKWTR
                     WDQDGRLAREWIRRLWFDARSELVYAATSESGVLRSLDGGRTWERRGAGLPIPDIRAL
                     AVDSDHPQRLYAGTYGRGVFVSEDGGGSWRGLNDVPVLSADAIIASLKVRDAGRPPSS
                     LVPPPAFAKCNGCHGWTDPEINLAPHSFWLVPPNRRDWRQTVHRMAPIANLSTAEEDE
                     VAAFLTAYSSQERP"
BASE COUNT          243 a          582 c          690 g          267 t
ORIGIN      
        1 gggagcgggt tttcgcggcc acggccgcca agggcgggcg gaccgggtac gtggccgcgc
       61 tggcgcaggc cggggagggg ctgttcgcgt ccgtcaacgg ccgcgtgtgg cggagccgcg
      121 acggccacgc gtggcgggag gtcggccagt tgacgccgtt taccgtggag gcgatgcacg
      181 aattcggcgg cgcgttgtac gcgggaacca cgatgccgcc tcgcgcctgg gtctaccgaa
      241 gccgggtatc cgatttgccg agccccgacg atcgcccggc gcctcgatga gcgggcgtgg
      301 gccaacggaa tgccctcgcc ggacagcggg ataaaggagt ccatgcgcac tcacgtgtca
      361 tcgccggccg gaccgtcctc gccacgcttg tccgggctcg ctcccgcgcg cgtgtgggga
      421 gcggcgctcg tggccgcgtg tctcgcgggg tgcgcgcgcg cgccgtcccc gccgccggcg
      481 acgagcgcgc cggtgtcgcc gcccgcgatt ttcgtctccc agatcgcgtt gcacccggcg
      541 cgccccgacc gggtgttggc gctgaccaac tacagcgtcg gattgctcgc gagccgggac
      601 cggggcgcgc actgggcgac cgcgaaccgc ggaatccgga gctattcgct ctaccggttg
      661 gtggtggatc cgcgcgatcc cgatctggtg tacgtgggcg cgggcggcgg agggttgtac
      721 cagagcgtgg acggggccaa gacgttcgtg gagcgcaaca acggcctggg caataccaac
      781 gtgggcgcga tcgtgctgca cccggtcgat cccgaccgcg tgttcgtcgt gacctccacg
      841 ggcgtgttca tcagcccgga ccgcggcgcg acgtgggccg cgtggaacca gggcgacgat
      901 ttcacgcaaa gccaacagtt ccaggatctc gtgatcctgc ccggcgagct ggagaccgtc
      961 ctgttggcgt ccaatcgcgg ggtgtgggtg cggcgcgcgg gtgacgccgc gtgggcgccg
     1021 gcgtcggggg atctccaggg ggtggcgatc acggtgttga ttcgacaccc ggacgggcgc
     1081 cgggtgtttg ccgcggcgct gcgcaacggc aagaccctgg aaggcggcgg actgtacgtc
     1141 aacgacacgt cgggcgccaa gtggacgcgg tgggaccagg acgggcgtct ggcccgcgag
     1201 tggattcgcc ggctctggtt cgacgcgcgg agcgaactcg tctacgccgc cacgtcggaa
     1261 tccggggtgt tgcggagcct ggacggcggt cggacgtggg agcggcgcgg cgcagggctg
     1321 ccgatccccg acatccgcgc gctggccgtg gattccgacc atccgcaacg gctttacgcc
     1381 ggcacgtacg gacgaggggt gttcgtgtcg gaagacggcg gcgggtcgtg gcgggggctc
     1441 aacgacgtcc cggtgttgag cgcggacgcg attatcgcgt cgctcaaagt gcgcgacgcc
     1501 ggacgccccc cgtcctcgct ggttccgccc cccgcgttcg ccaagtgcaa cggctgccac
     1561 gggtggacgg accccgagat caacctggcg ccgcacagct tctggctggt gccgccgaac
     1621 cgccgcgact ggagacagac cgtgcaccgg atggcgccga tcgcgaacct gtcaaccgcc
     1681 gaggaggacg aggtggcggc gtttttgacc gcgtactcgt cgcaggagcg accatgatgc
     1741 aggagcgctg gacgtcgtgg gtggtccggg ccgccgggtg ct
//
LOCUS       DBNGTT010000202         1779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1701847, whole genome shotgun sequence.
ACCESSION   DBNGTT010000202 DBNGTT010000000
VERSION     DBNGTT010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1701847"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="AC16BC_04565"
     CDS             complement(<1..984)
                     /locus_tag="AC16BC_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR3240923.1"
                     /translation="MTASNQSDRPTTDRLDTLLIHAGEPKPRIGGAVSMPIFQSANFE
                     YGGEASYDDLKYIRLNNTPNHMALHRKLAALEGGEAGLVTASGMAAISTSLLTVLSSG
                     DHLLAQACLYGGTHDLVTKDLPGFGISHDFIDANDPGSWARLLKPATRAIYVETMTNP
                     LLEVADLKAVVAFATQHKLVSMIDNTLASPINFRPLAMGFDLSIHSATKYLNGHSDIV
                     AGAVIGSKAWITRITHRLNHLGGSLDPHAAFLLHRGMKTLGVRMRHQNESALRIAEFL
                     AQHPAVASVNYPGLKSHPNHARAVELFSGHGGVLSVELRGGVDAADRLISRV"
     gene            complement(1109..>1779)
                     /locus_tag="AC16BC_04570"
     CDS             complement(1109..>1779)
                     /locus_tag="AC16BC_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3240924.1"
                     /translation="LPTMILPKRAVARHIARSWFAVVILGLVLTGPPEASATFQYTDD
                     AGTIHFTDDPGQIPEPIRKRMGIWVEPPETLPDTNLHLGSVDPSLSLDPDEPPNASTS
                     LWDQVVPTNIRSPWVPGLIGLAAAGSLLVGLRASMRPVSRVACKVAIVVVTAVSAAAT
                     VMAALAERGSSLPSMISEAQTTIDRWESRVTSPFKDAERRAKELGKAKREYQETIKQF
                     ESNP"
BASE COUNT          316 a          543 c          575 g          345 t
ORIGIN      
        1 cacccggctg atcagccggt cggccgcgtc cactccgccg cgcagttcaa cgctcaacac
       61 cccgccgtgc ccgctgaaca gctcaaccgc gcgcgcgtgg ttggggtggc tcttcaagcc
      121 cggataattc accgacgcca ccgccgggtg ttgcgccagg aactccgcga tgcgcaacgc
      181 gctctcgttc tggtggcgca tgcgcacgcc gagcgtcttc atgccccggt gcaggagaaa
      241 cgccgcgtgc ggatcgagcg acccgccgag gtgattgagg cggtgcgtga tccgcgtgat
      301 ccaggccttc gacccaatca ccgcgccggc cacgatatcg gaatggccgt tgagatattt
      361 ggtcgcgctg tgaattgaca gatcaaagcc catggcgagc ggccggaaat tgatcgggct
      421 cgccagcgtg ttgtcgatca tggagaccag cttgtgttgc gtggcgaacg ccaccaccgc
      481 cttcaaatcc gccacctcca gcaatggatt cgtcatggtc tcgacgtaga tcgccctggt
      541 ggcgggcttg agcaaccgcg cccacgatcc cggatcattc gcgtcgatga agtcgtgcga
      601 aatgccgaag ccgggcaaat ccttggtgac cagatcgtgc gtgccgccgt acaaacacgc
      661 ctgcgccagc agatgatcgc cggatgagag cacggtcaat agcgaggtgg aaatcgcggc
      721 catcccgctc gcggtcacca agccggcctc gccgccttcc aacgccgcaa gcttccggtg
      781 cagggccata tggttgggtg tgttattgag ccgaatgtat ttgagatcgt cgtagctcgc
      841 ttcgccgccg tattcgaaat tggccgactg aaaaatcggc atggacaccg ccccgccgat
      901 acgcggtttg ggctcgccgg catggatcag cagcgtgtcg aggcggtcag tggtcggacg
      961 gtcggactga ttcgatgcgg tcattgggta ttctctccgg gaaccccaga atatggtgag
     1021 cgggttcccg gggtcaagcg atgttcaagg agcctgttcg cctcagagcg gggtctcgaa
     1081 agaagcgcga cgcgtcagcc gtcgggtgct agggattgga ctcgaactgt ttgatcgtct
     1141 cttggtattc ccgttttgcc ttgcccagct ccttggcccg gcgctccgcg tctttaaatg
     1201 gagatgtcac gcgcgattcc cagcgatcga tggtggtttg ggcttcgctg atcatcgacg
     1261 gtagcgatga ccctcgttcg gcgagagcag ccatcacggt ggccgcggcg ctgaccgcag
     1321 tgacgactac gattgccacc ttgcaggcga ctcgggacac cggccgcatg ctggcgcgga
     1381 gtcccacgag gaggctgccg gctgcggcga ggccgataag tccgggcacc cacggcgagc
     1441 ggatgttcgt cggtaccacc tggtcccaga gtgacgtcga cgcgttcggg ggctcgtccg
     1501 gatccagcga gagcgaagga tccaccgatc ccaaatgcag gttcgtgtcc ggcaaggttt
     1561 cgggtggttc gacccagatc cccatgcgtt ttcgaatcgg ttccggaatc tgtcccgggt
     1621 cgtccgtgaa gtggatcgtc cccgcgtcgt ccgtgtactg gaaggtcgcc gacgcctcgg
     1681 ggggaccggt caagacgagc cccaggatca ctacggcaaa ccatgatcgt gcgatgtgtc
     1741 tggccacggc gcgtttcgga agaatcatgg ttggtaagt
//
LOCUS       DBNGTT010000203         2886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3235209, whole genome shotgun sequence.
ACCESSION   DBNGTT010000203 DBNGTT010000000
VERSION     DBNGTT010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3235209"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..>2886
                     /locus_tag="AC16BC_04575"
     CDS             232..>2886
                     /locus_tag="AC16BC_04575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3240925.1"
                     /translation="MIAVAVIAVRQIHIQRWTTLQTRNTEFHLEVVREISELTADLEK
                     ISHDLYRSPTPTTAQANMLDRALYEVNRRVDAIVVLHQRFAFDDAINALLRMQRAVSQ
                     LGETDRHLEHLDRNLIETVEARTASTRTRLVQLARLHERASDDIRRQLIVQEQRAFVT
                     LATVIVASAMISLAVMMVVLGHIRRLFARQAAADQAFYDSERRLRQILDSMNAFVGLF
                     SLDGRVIEANRTPLVAADLSLEEVVGTPVWETPLVSYSTETQAQVKSALARAAAGETV
                     REDIPVQVTGGQIVILDSTFAPLYNAQGRITMVIGSGVDVSARKQAEARHRTMSIALE
                     QTADNVLITDIHGRIEYVNHAFSHTTGYTAAEAIGRTPNLMKSGKHGTDFYRRLWETI
                     LAGAPFTDVFINRRKDGTFYSEEKTITPLKDAGGRITHFVSTGRDITERIQTQERLRF
                     LANHDALTGLPNRTLFLDRLEQAISRSRWRKRSVAVLFVDLDRFKNINDSLGHTVGDE
                     LLRALATRLSSALRDGDTVARFGGDEFVILLDDVANATDVGVVAKKVLDALTPPVNLG
                     TTLQVGASIGVSICPADGEDPGTLLKNADIAMYRAKEAGRNTCEFYSQEMSTRAFERM
                     TIESTIRQALARKEFTLHYQPQVDVRSGTIVAVEALLRWQHPDLGLVAPNQFIPVLED
                     TGRIVEVGEWLLAEACAQAARWRANGAPDLRMAVNLSGRQFLQPTLAAVVTEALARAG
                     VPPEALEVEITESMLMRPTDLTQQTIKTLDDLGVRFGLDDFGTGYSSLSYLQRFPFDT
                     LKIDRSFVRDLPADEHDAALARAILAMGRSLKLELVAEGVETDAQRDFLLAHQCVMMQ
                     GYLFSRPLPADEIGKLLRDPRRMLPRTDV"
BASE COUNT          548 a          911 c          902 g          525 t
ORIGIN      
        1 ggtttcgcag attcaggact gtctgttggc gctctccacg gatgaggcaa cggggcgccg
       61 cgttctagac gccgcggacg ccgggagctt tcttcccgcg cttgatagcg actttaatcg
      121 cctgcgcccg gcaacggcgg tgttagacgc tccagaactc gtccgatgac acgctccagc
      181 acgcaagcga ccgttttacg ccagatctac ctgcttatcg gcctgataac cttgatcgcc
      241 gtcgccgtca ttgccgtccg tcagatccat atccagaggt ggacgacgct tcagaccagg
      301 aacacggaat ttcacctgga ggtggtgcga gaaatctcgg agctgaccgc ggatcttgag
      361 aagatttccc acgatctgta ccgatcgccg actcccacaa cagcgcaagc caacatgctt
      421 gaccgtgccc tgtacgaggt caaccggcgc gtcgacgcca tcgtcgtgct tcatcaacgg
      481 tttgccttcg acgatgccat caacgccttg ctgcggatgc agcgcgcggt gtcgcaactc
      541 ggtgagaccg atcgtcacct cgagcacctg gaccggaatc tgatcgagac ggtcgaggct
      601 cgaaccgcat ccacgcgcac gcggctggtg caattggcga gactgcatga acgcgcgagc
      661 gacgacattc gccgccagct catcgtccag gaacagcggg cgtttgtcac gttagccacg
      721 gtcatcgtgg cgtctgccat gatcagcttg gcggtcatga tggttgtcct cggacatatt
      781 cgtcgcctgt tcgcgcgtca ggcggccgca gaccaagcgt tttacgacag cgagcggagg
      841 ctccgccaga tcctagacag catgaacgcg ttcgtgggcc tcttttcgtt ggatggccgc
      901 gtgatcgaag cgaatcgaac gccactcgtg gccgcggatc tctccttgga agaggtggtc
      961 gggacgccgg tctgggagac accgttggtc tcctattcaa ccgagacgca ggcgcaggtc
     1021 aaatcggcgc ttgctcgcgc agcggccggc gaaaccgtcc gtgaagatat tcccgttcaa
     1081 gtcaccggcg ggcagatcgt gatcttggac tcgacgttcg cgccactcta caacgctcag
     1141 ggccggatca cgatggtgat cggatccggg gtggatgttt cggcccggaa acaggccgag
     1201 gcccggcatc ggacaatgtc catcgcgctg gagcaaaccg cggacaacgt gctgatcacc
     1261 gacattcacg ggagaatcga gtacgtcaac cacgcctttt cgcatacgac cgggtacacg
     1321 gcggcggagg cgatcgggcg tacacccaac ctgatgaagt ccggcaaaca cggtaccgac
     1381 ttctaccgcc gcctgtggga gacgatcctg gccggcgcgc cgttcaccga cgtgtttatc
     1441 aaccgccgga aggacggcac gttctactcc gaggaaaaga ccatcacgcc gctcaaggac
     1501 gccggcggac gcatcaccca tttcgtgtcc accggacggg acatcaccga gcgcattcag
     1561 acccaggaac ggctccgctt cctggccaac cacgacgcgc tcacgggctt gccaaaccgc
     1621 acgctgttct tggaccgtct ggaacaggcc atcagccgtt cgcgttggcg caagcggtcg
     1681 gtggcggtgc tgttcgtgga cctggaccgc ttcaagaaca tcaacgactc gttggggcac
     1741 accgtgggcg atgaactctt gcgcgccctg gcgacccgtc tgtcgtccgc cttacgggac
     1801 ggagacaccg tggcgcgttt cggcggcgat gaattcgtga tcttgctcga tgacgtggcg
     1861 aatgccacgg atgtcggcgt cgtggccaaa aaggtgctgg atgccctcac tcccccggtt
     1921 aatctgggaa ccacgctgca ggtcggggcc agcatcgggg tgagtatctg ccccgcggac
     1981 ggcgaagatc ccgggacgtt gctcaagaac gcggacatcg ccatgtaccg cgcgaaagaa
     2041 gccggacgca acacctgcga gttctattcg caggaaatga gtacgcgggc cttcgagcgg
     2101 atgaccatcg agagcacgat ccggcaagcg ctcgcccgca aggaattcac tctccattac
     2161 cagccccagg tcgacgtgcg gagcggaacg atcgtcgccg tggaagcgct cttgcgctgg
     2221 caacacccgg atctggggct cgtggctcca aaccagttca tcccggtgtt ggaggacacc
     2281 ggccgcattg tggaggtggg agaatggttg ctcgccgaag cgtgcgcaca ggcggcgcgc
     2341 tggcgcgcga acggggcccc ggatctccgc atggccgtca atctgtcggg gcgccagttt
     2401 ctccagccga cgttggccgc cgtggtgacg gaggccttgg cgcgtgccgg ggtgccgccc
     2461 gaagccctcg aggtcgaaat caccgagagc atgctgatgc gcccgaccga cctcacgcag
     2521 cagacgatca agaccctgga tgacctcggt gtccgtttcg gtctggacga cttcgggacc
     2581 ggatactcgt cgctcagcta ccttcagcgt ttcccgttcg acacgctcaa aatcgaccga
     2641 tcattcgtgc gcgacctccc ggcggacgaa catgacgcgg cgttggctcg ggcgatcctg
     2701 gccatggggc ggagcctcaa actggagttg gtggccgagg gcgtcgaaac cgacgcgcaa
     2761 cgggacttcc tgctcgcgca ccagtgcgtc atgatgcagg gatatctctt cagtcggccg
     2821 ctgccggccg acgagatcgg taaattgttg cgggacccgc gacggatgct cccccgcacg
     2881 gacgtt
//
LOCUS       DBNGTT010000204         5892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2340665, whole genome shotgun sequence.
ACCESSION   DBNGTT010000204 DBNGTT010000000
VERSION     DBNGTT010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2340665"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="AC16BC_04580"
     CDS             complement(<1..677)
                     /locus_tag="AC16BC_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240926.1"
                     /translation="MRHQGRHLRLLLSIAALAAVVTWSQTGLTQPPPPPPPVSQQVLE
                     DITNTKHNLSSIDPAQLLSSGVFPRPGSARDAKAAPGGTTEICVFCHTPHGASATGGG
                     FKAPIWNRSVDYQASQTYQLYDQVWSFSFEGTLNDAAGRRPTGYSRLCLSCHDGTVAL
                     GNLLNAPGSGGYQGRVAMTGNLGAGGTIPHGSGDLTGDTRRLGLDLRNDHPISFAFDQ
                     ALLLQDQ"
     gene            complement(853..1833)
                     /locus_tag="AC16BC_04585"
     CDS             complement(853..1833)
                     /locus_tag="AC16BC_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3240927.1"
                     /translation="MWPLPPDPPRIAYVKALTQPEPSQKPKGFFTRLFERVVGKGEEI
                     APRMIRPFALFTDDAGALFVTDMGLQVVHRFGADGAYRQFFRMDNGRRLLSPTGVTAD
                     RDGVVYVADSQINRVVVFNRDGAYQREFGAGDAVRVSGIAYHRGRDALYVSDAGGHKV
                     RIYSRDGRKLGEFGSRGPDAGQFNFPTHLTVDRAGRLYVTDAMNFRIQVLEPDGRWVR
                     SIGQLGTTLGSFSKPKGVGLDQHGNVYVVDGLYDTVQIFNADGDLLMNFGNAGTTEGA
                     FWLPTGVTVDGQDRIYVADTYNARVQVFRLVDASPRSETPSGGTGEGGSR"
     gene            complement(2106..3980)
                     /locus_tag="AC16BC_04590"
     CDS             complement(2106..3980)
                     /locus_tag="AC16BC_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240928.1"
                     /translation="MNAPRRIPLRPAGLVAVILAAGALFGAPPAQARVLNINGLVELT
                     GRWSRWDHSVVSAALPPPVTNGIQQRYQLGSNGDLYHPNLGTYIANISLIDDVGRVDG
                     DKQQDLTVKDFYVNVSLLPRVSPVTIYAQRIVQDQERIEARTLGVGTTTTTYNLTWDF
                     PVFRNLPRLRLNLSQNEIETATTQIDNLAIPPTQKDVTSGQRTRAAALDVDGQAGNTR
                     YFARTQFTQLHGDFVGTDSVSLTASTDTRFSPALSAATRLNYSTSVETLGVVTPGTGT
                     LQQRSAGASVFYRPSLQTTLSGMYDYYKDPFVRHLATGSAILRPIQELDLSAGYRLSR
                     FDVPFALTTSHYAYAAANYRPFLGLSTNATASVGWTDVTGITDITSLYQSYGYGANYF
                     KTLTLITYRLGYQGNYSQNRLNTDRGTSFDWTNVFTAGLSNTQTRLVSLSGDYALSLV
                     RHRTVGAPTTDQVDHRVQATATSSAPQNLFLTGDFMVLTGMASYTLTQFANFSNHVLI
                     LTTTDTYETGRGVAATVGYTFEQQSQLEYDNKSTSFIQVRWLSYIVQNGSLDLTVKQV
                     WERYAGNQQDVSRSEGGALFSYYLGKVSLSADYRLTYETRPADRQLYQTAFAKVSRPF
                     "
     gene            complement(3977..4927)
                     /locus_tag="AC16BC_04595"
     CDS             complement(3977..4927)
                     /locus_tag="AC16BC_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3240929.1"
                     /translation="MLIVAALAGGPRLGQAADDRVAARVNGVPIMLAHVDRAVDEKVP
                     RITGHGVLSDSRRRVLRAQVLENLIDEELMVQEARRRKLTADASAVDEETAKIRKRFE
                     DSKQYADALSRAGLSESDVRTGVERYLLVKRVTELAVTAKVTVTDASMRAYYDADPSR
                     FVVPEQVRYRQIVIGVDPGGSPAEWDAARQRAAGLAKQARGGKSFADMAKTQSDDAKS
                     RESGGDMGWVHRGRLEHDDDAAIFALPPGGVSDPVRTLYGYAIYRVEEKKKAKALGWD
                     EVNKTRLADELRRAETDRVRAEWLGDLRQRARVEVLPAEP"
     gene            complement(5068..>5892)
                     /locus_tag="AC16BC_04600"
     CDS             complement(5068..>5892)
                     /locus_tag="AC16BC_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240930.1"
                     /translation="DVAGGGAPLWNRALPPSNQFTNYTSPNFDNDLGTETAGPKGVSL
                     ACLSCHDGVIALDALINAPGSGGFNASNRNPLTGTSSGQGLDGITFNSVSPGVDADGS
                     LREGDRPSSTAGGCYNENPNANGLDCLVGGNTGMAPFPNLGRNLTDDHPISMRMPSTD
                     PQFTDALTSSTTYSPGGNVRYVSRSGAQLPPDSRDRIRLYNSGNDGTGTTIDWVECAS
                     CHNPHTPRTTFLRLPSTPAGISAPGDGEVVAAAGATRNLNHEPNQGSLICLTCHQK"
BASE COUNT          928 a         1882 c         2024 g         1058 t
ORIGIN      
        1 tcctggtctt gcagcagcaa cgcctggtcg aacgcgaacg agatcggatg gtcgttgcgc
       61 aggtcgagcc ccaggcggcg ggtgtctccg gtaaggtcgc cgctgccgtg gggaatggtg
      121 ccgcccgcgc ccaggttgcc ggtcatggcg acgcggccct ggtagccgcc ggagcccggc
      181 gcgttgagca ggttgccgag cgccacggtg ccgtcgtggc acgagaggca caggcgcgag
      241 tacccggtgg gccggcgccc cgccgcgtcg ttgagcgtgc cttcgaacga gaagctccac
      301 acctgatcat agagctggta cgtctggctg gcctgataat ccacgctgcg attccagatc
      361 ggggccttga agccgccgcc cgtggcgctg gcgccgtgcg gggtgtggca gaacacgcag
      421 atttcggtgg tgcctccggg cgcggctttc gcgtcgcggg ccgagccggg acgcggaaac
      481 acgccggacg agagcagttg cgcgggatcg atcgacgaga ggttgtgttt ggtgttggtg
      541 atgtcctcca acacttgttg gctcaccggc gggggcggcg gaggcggctg ggtcaggccg
      601 gtctgcgacc acgtcacgac cgccgccagc gcggcgatgg aaagtagaag gcgaagatgc
      661 cgtccctggt gcctcatgcc actctcccgt gtaagagacg aaccggtgaa ccggggaacc
      721 ggcgaaccgg ggaaactgat gaaacatgaa ccggtgaatc gacgaaccgg ggaatcggcg
      781 cagtgacggc gaatcctcgc ttttgacctt tcaccgtttc tccgattcgc ccattctccg
      841 agtcgattgc tctcaccggc tccccccttc cccggttccc ccgctcgggg tttcactccg
      901 aggcgacgca tccacgagcc ggaagacctg cacgcgcgcg ttgtacgtgt cggccacgta
      961 gatccgatcc tgtccgtcca cggtcacgcc ggtggggagc cagaacgcgc cctcggtcgt
     1021 gcccgcgttg ccgaagttca tgagcaggtc gccgtccgcg ttgaaaatct ggaccgtgtc
     1081 gtacaggccg tccaccacgt aaacgttccc gtgttgatcc agccccacgc ccttgggttt
     1141 ggagaaactt cccagggtgg tccccaactg cccgatggac cgcacccagc ggccgtccgg
     1201 ttccaggacc tggatacgga aattcatcgc gtccgtgacg tagaggcggc ccgctcgatc
     1261 cacggtgagg tgggtgggga aattgaactg ccccgcgtcc gggccccggg agccgaattc
     1321 cccgagcttg cggccgtcgc gcgagtagat gcgcaccttg tggcctccgg cgtcgctcac
     1381 gtagagcgcg tcacggccgc ggtggtaggc gatgccggag acgcgcaccg cgtcgccggc
     1441 gccgaactcg cgttggtagg ccccgtcgcg gttgaacacc accacccggt tgatctgcga
     1501 atccgccacg tacacgacgc cgtcgcgatc cgcggtgacg cccgtggggg agagcaggcg
     1561 ccgcccgttg tccatgcgaa agaattgccg gtacgcgccg tccgcgccga accggtgcac
     1621 gacttgaagg cccatgtcgg tgacgaacag ggcgcccgcg tcatccgtga agagcgcgaa
     1681 cggacggatc atgcgcggcg cgatttcttc gcccttgccc acgacgcgtt cgaacaatcg
     1741 ggtgaaaaag cccttgggtt tctggctcgg ctcgggttgc gtgagcgctt tgacgtacgc
     1801 gatccgcggc ggatcaggcg gcagcggcca cacgcgcgcg accgcgggcg cggaaggtgc
     1861 cgccggcgcg cccgcgcagg acgcgaggag cagcagcgaa ccggcgagga ggggaaaaga
     1921 cgagtgggtg agtgggagag ggggtgaggg gggggatggg cgaaaggccg caagaaagcg
     1981 tgagtggacg agagaggtca cggctgtccg ccgtttcacc ggttcaccgg ttctccccat
     2041 cgccccaccg tcctttcccc ggctcaccga ttcccccctt ccccccatcg ctgttccgcc
     2101 gtttactaaa acggtcgcga gaccttcgca aacgcggtct ggtagagctg cctgtcggcc
     2161 gggcgcgttt cgtacgtgag ccgatagtcc gcgctcaggg agaccttccc caggtagtag
     2221 gagaacaagg ccccgccttc ggaccggctc acgtcctgct ggttgcccgc gtaccgctcc
     2281 cacacctgct tcacggtcag atccagcgaa ccgttctgca caatatacga taaccaccgc
     2341 acctggataa agctcgtgga cttgttgtcg tattcgagct gggattgctg ctcgaaggtg
     2401 tacccgacgg tcgcggccac gccgcggccg gtctcgtacg tgtcggtcgt ggtcaggatc
     2461 agcacgtggt tggaaaagtt cgcgaattgg gtgagcgtgt acgaggccat gccggtcagc
     2521 accatgaagt cgccggtcag gaacagattc tgcggcgcgc tgctggtggc cgtcgcctgc
     2581 acgcggtgat cgacctggtc cgtggtcggg gcgccgaccg tgcggtgccg gaccagggac
     2641 agcgcgtagt ccccggagag cgacaccaga cgggtctgcg tgttgctcag cccggcggtg
     2701 aacacgttgg tccagtcgaa cgacgtgccg cgatccgtgt tcagtcggtt ctggctgtag
     2761 ttgccttggt agccgaggcg ataggtgatc agcgtgagcg tcttgaagta gttcgccccg
     2821 tacccgtaac tctgatacag gcttgtgata tcggtgatcc cggtgacgtc ggtccatccc
     2881 accgacgcgg tggcgttcgt cgacaagccc aggaacgggc ggtagttggc ggcggcgtag
     2941 gcgtagtgcg aggtggtgag ggcgaagggc acgtcaaacc gcgacagccg gtaccccgcg
     3001 gacagatcca actcctggat cgggcgcagt atggcgctgc ccgtggccag gtggcggaca
     3061 aagggatcct tgtagtagtc gtacatcccg ctcagcgtgg tctgcagact gggccggtag
     3121 aacacgctgg cgcccgcgct gcgctgctgc agcgtgccgg tgccgggcgt caccacgccc
     3181 agcgtttcca cgctcgtcga atagttcagg cgcgtcgcgg ccgacaacgc gggggaaaaa
     3241 cgggtgtcgg tcgaggccgt caggctgacg ctgtcggtcc cgacgaagtc gccgtgcagt
     3301 tgggtgaact gcgtgcgcgc gaagtaccgg gtgttgccgg cttgcccgtc cacgtccagc
     3361 gcggcggcgc gcgtgcgctg gcccgacgtg acatcttttt gggtcggcgg gatcgccaag
     3421 ttgtcgatct gggtcgtggc cgtttcgatt tcgttctgcg agagattcag tcgtagccgc
     3481 gggaggttgc gaaatacggg gaagtcccag gtcaagttgt atgtggtggt ggtggtccct
     3541 actccgaggg tcctggcctc aatgcgctcc tggtcctgca cgatccgctg ggcgtagatg
     3601 gtcaccgggc tcacgcgcgg gagcaggctc acgttgacgt agaagtcctt gaccgtgagg
     3661 tcctgttgct tgtcgccgtc cacgcgcccc acgtcgtcga tcagggagat gttcgcgata
     3721 tacgtgccga ggttgggatg gtacaggtcg ccgttgctgc cgagttgata ccgttgctgg
     3781 atgccgttgg tgactggggg cgggagggcg gcgctgacca cgctgtgatc ccatcgcgac
     3841 catcgccccg tcaattccac caggccgttg atgttcagga cgcgcgcttg cgccggcggg
     3901 gcgccgaaca gcgctcccgc ggcgaggatg acggcgacga gtccggccgg cctgagcggg
     3961 atgcggcggg gggcgttcat ggttcggccg gtaggacttc caccctggcc cgctgtctga
     4021 ggtcgcccag ccactccgcg cggacccggt cggtttccgc ccgccgcagc tcgtcggcca
     4081 accgcgtctt attgacctcg tcccacccca acgccttcgc cttctttttc tcctcgaccc
     4141 gataaatcgc gtagccgtag agagtgcgca ctgggtcgct cacgccgccg ggtggcagcg
     4201 cgaagattgc cgcgtcgtca tcgtgctcca gccggccccg gtggacccag cccatgtcgc
     4261 cgccggattc gcgactcttc gcatcgtccg actgcgtttt ggccatgtcc gcgaaggatt
     4321 tcccgccgcg ggcttgtttg gccagacccg cggcgcgttg ccgggccgcg tcccactccg
     4381 cggggctgcc gccggggtcc acgccgatca cgatctggcg atagcggacc tgttcgggaa
     4441 ccacgaaccg ggacggatca gcgtcgtagt aggcgcgcat cgacgcgtcg gtgaccgtga
     4501 ccttggccgt gacggccagc tcggtcacgc gtttgaccaa gaggtatcgc tccacgccgg
     4561 ttcgcacgtc ggattcggac aggccggcgc gcgagagcgc gtccgcgtac tgcttggaat
     4621 cctcgaaccg cttgcggatc ttcgcggtct cctcgtccac ggcggaggcg tccgcagtca
     4681 gtttgcggcg cctggcttcc tgcaccatga gttcttcgtc gatcaagttt tccagcactt
     4741 gggcccgcaa cacgcggcgc cgcgagtccg agaggacgcc gtgtcccgtg atgcgcggca
     4801 ccttctcgtc cacggcacga tccacgtgcg ccagcatgat gggcacgccg ttgacccgcg
     4861 ccgcaacccg gtcgtcggcg gcctggccca ggcgcggacc gcctgcgagg gccgcgacga
     4921 tcaacacgcc ggttcgcgct ccggttgcca gcggcccggc cagggcgtgc cgtaaaagcg
     4981 gtttcgccca ggccgaagcc tgggcgaatg gactcggatc agggagcgcg ggtaaagccg
     5041 gggtcgatct cgaccccggt cgacccgtta tttctggtga cacgtgaggc agatcaagct
     5101 cccttgattg ggttcgtggt tcaggttacg cgtggcgccc gccgccgcga ccacctcgcc
     5161 gtcccccggg gccgagattc cggcgggcgt actgggcagg cgaagaaacg tagtccgcgg
     5221 agtgtgcgga ttgtggcacg acgcgcactc gacccaatca atggtggttc cggtcccatc
     5281 gtttccgctg ttgtagagcc ggattcgatc acgggaatct ggcggcaact gggctccgga
     5341 tcgcgagacg taccgcacgt tcccgcctgg ggagtaggtg gttgaactgg tcagagcatc
     5401 ggtaaactga gggtccgtgc tcggcatgcg catcgaaatc ggatgatcat cggtcagatt
     5461 ccggcccaag ttcgggaacg gggccatccc cgtatttccg ccgacaagac aatccaaccc
     5521 attcgcattc gggttctcgt tgtaacagcc gcccgcggtt gaactggggc gatccccttc
     5581 ccgcagggag ccgtccgcgt ccacaccggg gctgacgcta ttgaaggtaa tcccgtccag
     5641 cccctggccc gacgatgtgc ccgtgagggg gtttcggttg gacgcgttga atcccccgga
     5701 tccgggggcg ttgatcaggg cgtccagcgc gatgaccccg tcgtgacagg acaggcacgc
     5761 caacgacacg cccttgggac ccgcggtctc tgtcccgaga tcgttatcga agttgggcga
     5821 cgtgtagttg gtgaactggt tcgagggagg cagcgcccgg ttccacaacg gcgcgccgcc
     5881 cccggccacg tc
//
LOCUS       DBNGTT010000205         4335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1958549, whole genome shotgun sequence.
ACCESSION   DBNGTT010000205 DBNGTT010000000
VERSION     DBNGTT010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1958549"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..563
                     /locus_tag="AC16BC_04605"
     CDS             129..563
                     /locus_tag="AC16BC_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR3240931.1"
                     /translation="MNRELKQRRQSEAGFTLLELLIAVTILAVGLMATVNMETVALRA
                     DAFAQRISSSAAVARGAMEELVSRDGSDAIFQTAQVNAAYDLDLQTAATTVTVQGVTY
                     SATISITPNATVNGVAVTNLTQIALTVTGADRTVTLTELKRT"
     gene            563..1525
                     /locus_tag="AC16BC_04610"
     CDS             563..1525
                     /locus_tag="AC16BC_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR3240932.1"
                     /translation="MKQRIHNARQFPQSGFSLIELLIVLAIFGVVIGAINNLYVVHQR
                     AATVEGEAVDVQQNVRIGLEQMENDISMAGFLVLGADPIAVAADGGAPGIPDTLTLNT
                     GTESLTAATVNTSNPAVVVAAGTVLDLPVIPYGGSIGGFEASADQGVAQVRIVNFRSE
                     PLGVGTTFTVLLVNPAAGACNAVPAPCMSLRANGPGSGSISTGDSIVKTSVLGAAAEA
                     FPHSVTYSVAVCPAPLQGNCLMRTTVPTPAAGPAVVATNVSDLQLRYLLAGNLEVDAP
                     TAAQMASIRAVRITMTGQLVSTVQAAGGNQKPRTLSTVVAIRNT"
     gene            1537..1998
                     /locus_tag="AC16BC_04615"
     CDS             1537..1998
                     /locus_tag="AC16BC_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIR3240933.1"
                     /translation="MNTRTDEHGVALVLALWMLVILGLVGTLLLSSSNVEMRLAANNR
                     NMRTALYTSDAAVQYAKSDSTIYTTIGSGAGAFPAGATPGTYTLNNVPIGANSAQRIN
                     VAYLTVGPPPPGSGIDASKYQTVYFSAQAVGVGPNNSQALVEVQIAKPILK"
     gene            2010..2504
                     /locus_tag="AC16BC_04620"
     CDS             2010..2504
                     /locus_tag="AC16BC_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240934.1"
                     /translation="MTRQHRVARFTRQLSAIAVSVAVFMTVLQVPSHAEQGFGAPIGP
                     QETRHSWNVEAHMNVAPVADSDRRRDEYGLYNADTAYNGVYDRARLYRVDLSSQAWVG
                     SLLPPSLPRVTCRPARAALEHQGTWTGALKSDGSCGVGEPTDWAVGNFLNFDANLHET
                     DSEL"
     gene            2549..>4335
                     /locus_tag="AC16BC_04625"
     CDS             2549..>4335
                     /locus_tag="AC16BC_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HIR3240935.1"
                     /translation="MNTILRTLLLAAVALIAPALATAAPDFYVGDTAIYGGAPANVDP
                     NVVILFDTSLSMNDATLPENPYDPGTTYAAASGCGGQPCVTNTVYKCTAFGLECGNWT
                     TNTVDVSTVTTSCPVGTNPKNSLLTTGQWNSSRKKLQTSGACASGNGIYALGNWINWR
                     SSLPPGAFSPKIDIAKQVVTDLLNSTTGIRFSLFDFNNNQGAKVLDGSNGYRATVKDM
                     DALFDATTTNRDALVYAVNKDIVADSWTPLAESLYEVGRYYQGKVSAFTTESWTSPIQ
                     ASCQKNYVIIVTDGMSTADQDNVLKTICNNGDCDGDGFEPANDPAKTYPNQGSDYLDD
                     VAKYLHDTDVSTTFDGTQNVITFTVGFGLGGSNAGAVKLLSETAANGGGKAYLSNSAS
                     ELSTALQQILGQIMEVNSSFVAPVVPVSPENRTYSSGRVYLGFFKPQSGTSMWLGNLK
                     KYGINDTGVVVDSTGTVSNYVDRNRDGKDDNTGATLPSGATDGSFKATAASYWTSVPD
                     GGDVDNGGAGEVLLNRDFASNPRKIYTYTGTNVSLTDASNAVTTTNAAITPAMVGVAT
                     AADHDKLIKYVHGWDSYDADVDGVVDEKRT"
BASE COUNT          921 a         1326 c         1310 g          778 t
ORIGIN      
        1 tgccccacag tggataagaa cgtcccaatc aggctctcgt ctttgaatgg cagatatatc
       61 aattagttgt gtgcagtcct ggagaggcac agaacgtgct tttcaggtgg gcaactggag
      121 caaacggtat gaacagggaa ctgaaacagc gacgacaatc cgaagccggg ttcactctac
      181 tggaactgct cattgccgtc acaattttgg cagtcgggct tatggccacg gtcaacatgg
      241 aaaccgtcgc ccttcgagcc gacgcattcg ctcaacggat tagctcctca gcagcagtgg
      301 cccgcggcgc gatggaagag ctcgtatcgc gcgacggatc cgacgcgatc tttcaaacgg
      361 cacaagtcaa cgccgcgtat gacctcgatc tccaaaccgc ggccacgacg gtaacggtcc
      421 aaggggtgac ctattccgca accatcagta tcacccccaa tgcgacagtc aacggcgttg
      481 ctgtaaccaa tttgacacaa attgcgttga ccgtcacggg tgccgatcga accgtgacac
      541 tgaccgaact caagaggacg tgatgaaaca acgcattcat aacgccaggc aatttcccca
      601 atccgggttc agcttgattg aactgttgat cgtgctggcg atcttcggcg tcgtgatcgg
      661 cgcgatcaac aacctctacg tggtgcacca gcgggccgcc acggtcgagg gcgaggcggt
      721 ggacgtgcaa caaaatgtcc gaatcggact cgaacagatg gagaacgata tctccatggc
      781 tggtttcttg gtgctcggcg cggacccgat cgccgtggcc gcggacggag gggcccccgg
      841 aatcccggat accctgacgc tgaacacggg cacggagtcg ctgaccgcgg ccaccgtcaa
      901 cacgtcgaat cccgcggtcg tggtggctgc aggaaccgtg ctcgatctgc cggttattcc
      961 ctatggcggg tccatcggcg gcttcgaggc ctctgcggat caaggtgtcg cccaggtcag
     1021 gatcgtcaat ttccggagcg agcccctggg cgtgggcacg acctttaccg tgctgctcgt
     1081 caaccccgcg gctggcgcct gtaatgcggt gccggcgccg tgtatgagcc tccgagcgaa
     1141 cggtcccggg agcggatcga tctcaaccgg cgacagcatc gtgaagacga gcgtgcttgg
     1201 cgcggccgcg gaagccttcc cccattcggt gacgtactcg gtcgcggtgt gccccgcgcc
     1261 gctccagggc aattgcttga tgcggaccac ggtcccaacg ccggcagccg gccccgcggt
     1321 ggtcgcgacc aatgtgagcg atcttcagtt gcgatatctg ctcgccggta atctcgaagt
     1381 ggacgcgccg accgccgcgc aaatggcgtc cattcgagcg gtccgtatca ccatgaccgg
     1441 ccaactcgtc tccacggtcc aggcagcggg cgggaaccag aaaccgcgaa ccctgagcac
     1501 cgtggtggca attcggaaca cctgatggag gacactgtga acacacgaac ggatgaacac
     1561 ggagtcgccc ttgtcctggc cctgtggatg ttggtgatcc tcggcctggt cggcaccttg
     1621 ctcttgtcgt cgtccaacgt ggagatgcgg ctggcggcga ataaccgcaa tatgcggacc
     1681 gcgctctaca cctcggatgc ggcggtccag tatgccaagt cggactccac gatttacacc
     1741 acgatcggaa gtggggccgg cgcttttcca gcgggagcca cgccgggaac ctacacgctc
     1801 aacaatgtcc cgatcggcgc aaactcggcg cagcgcatca acgtagcgta tctcacggtc
     1861 gggcctcctc cgcccgggtc cggaatcgac gcctcgaaat atcagaccgt gtacttctcg
     1921 gcccaggcgg tgggagtcgg gccgaataat tcgcaggccc tggtcgaagt gcagatcgcg
     1981 aagccgattc taaagtagga gggcatgcca tgactcgtca gcatcgtgtc gctcgattca
     2041 cgcgccaact ctcagcgatt gcagtgagcg tggccgtctt catgaccgta ctccaggttc
     2101 ccagccatgc cgagcagggc ttcggcgccc cgatcggacc gcaggaaacc cggcactcgt
     2161 ggaacgtgga ggcgcacatg aacgtcgccc cggtggcaga cagcgaccgc cgtcgggacg
     2221 agtacggtct gtacaacgcc gataccgctt ataacggggt ctacgaccga gcccggctct
     2281 atcgggtgga cctctcatcg caagcgtggg tcggcagcct tctcccgccc agcctgccac
     2341 gcgtcacctg tcgcccagcg cgggctgcgc ttgaacacca aggcacctgg accggggcgc
     2401 tgaaatccga tgggtcctgt ggggtcgggg aaccaaccga ctgggccgtg gggaatttcc
     2461 tgaactttga cgccaacctt cacgagaccg actcggagct ataaccatgc gttctcggaa
     2521 accgggtcat ccgatgggga ggttcactgt gaacaccata cttagaacgc tgctcctggc
     2581 cgcggtggct ctgatcgccc cggctcttgc gacggccgcc cccgacttct acgtgggtga
     2641 tacggctatt tacggcggcg cgcccgccaa tgtcgatccg aacgtggtga tcctcttcga
     2701 tacctcgttg agcatgaacg acgccacatt acccgagaat ccgtacgacc ctggaacaac
     2761 ctacgcggcg gccagcgggt gcggcggcca gccgtgcgtc accaacacgg tgtacaagtg
     2821 cacggcgttc ggcttggagt gtggaaactg gaccacgaac acggtagacg tgagcacggt
     2881 caccacctcg tgtccagtgg gcacgaaccc gaagaattct ctgctgacca ccggccagtg
     2941 gaacagcagc aggaagaaat tgcagacctc gggcgcgtgc gcgagcggga acgggattta
     3001 cgcgttggga aactggatca actggcgttc gagcctgcct cccggcgcgt tctcgccgaa
     3061 aatcgatatt gccaaacagg tcgtgacgga cctgctcaac agcaccaccg ggattcggtt
     3121 cagcctgttc gattttaaca acaaccaggg cgcaaaggtg ttggacggca gcaatggata
     3181 cagggccacg gtcaaggaca tggacgcctt gttcgatgcc accaccacca atcgagacgc
     3241 actggtgtac gcagtcaata aggacattgt cgcggattcg tggactccac ttgccgaaag
     3301 tctctatgaa gtcgggcgct actatcaagg caaggtcagc gccttcacca ccgaaagctg
     3361 gacgtcgccg atccaggcct catgccagaa aaattacgtc atcatcgtga ccgacggcat
     3421 gtccactgcg gaccaggata acgtcctcaa gacgatttgt aataatggcg attgcgatgg
     3481 cgacgggttc gaaccggcaa atgacccggc caagacctac ccgaaccagg gctcggacta
     3541 tttggacgac gtggccaagt acctgcatga cacggacgtg tccacgacct ttgacggcac
     3601 gcaaaacgtg atcactttta ccgtagggtt cgggctcggc gggtcgaatg cgggcgcggt
     3661 caagctgctg tctgaaaccg cggccaacgg cggcgggaaa gcctatctct caaacagtgc
     3721 cagcgagctt tcgaccgcgc ttcaacagat cttgggccag atcatggaag tgaactcctc
     3781 gttcgtggcg ccggtggtgc cggtcagccc agagaaccga acatacagct ccggccgcgt
     3841 gtacctgggc ttcttcaagc cccagagcgg cacgtcgatg tggttgggca atctcaagaa
     3901 atatgggatc aacgataccg gagtggtggt tgactcgacc ggcacggtgt ccaactacgt
     3961 ggaccggaat cgagacggca aggacgacaa caccggcgcc accctgccga gtggggcaac
     4021 cgacggaagc tttaaggcga ccgcagcctc ctattggacg tcagtgcccg atggcggcga
     4081 cgtggacaac gggggcgcgg gcgaagtcct ccttaaccgc gacttcgcct ccaacccgcg
     4141 caagatctac acgtacaccg gcaccaacgt gtcgttgacg gatgcctcga acgcagtgac
     4201 aaccaccaat gccgcgatta caccggctat ggtgggggtc gcgaccgcgg ccgaccacga
     4261 caagctgatt aagtatgtcc acggctggga ttcatacgat gcggatgtgg atggcgtggt
     4321 cgatgaaaaa cgcac
//
LOCUS       DBNGTT010000206         5360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_169662, whole genome shotgun sequence.
ACCESSION   DBNGTT010000206 DBNGTT010000000
VERSION     DBNGTT010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_169662"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="AC16BC_04630"
     CDS             <1..1293
                     /locus_tag="AC16BC_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute symporter family protein"
                     /protein_id="HIR3240936.1"
                     /translation="LAYRLSPRPVRAMASLSTLTVSTFYMIAQMVGAGALVALLLKDS
                     GLSFEAAVIGVGVLMIVYVVFGGMLATTWVQIIKAVLLMGGTVLLSVLVLVHYGFDLG
                     EFFRAASHVTYHDKSGQAITQNFLEPGLRFKPPYGALDLISLGLALIFGTAGLPHILV
                     RFYTVPDAKTARVSVVWAMAIIGSFYIMTTFLGFGAATIVGRDAITANGGTNMSAQLL
                     AQALGGDVFFAFISAVAFATILAVVAGLTISASTSFAHDFYTNVLHHGRERSPGEEVR
                     VARVTACVVGVLSMAIAIVVGPNTNVAFLVALAFAVAASANLPVIVFSLFWKRFTTRG
                     AVWGLATGLISSIALILVSPSVMTIDAPGVTGAARHLIQAAPLFPLENPGIASIPLGF
                     LGAIVGTLLGREPQAEAKYAELLVRANTGLGAEQPSGR"
     gene            complement(1382..2140)
                     /locus_tag="AC16BC_04635"
     CDS             complement(1382..2140)
                     /locus_tag="AC16BC_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dph6-related ATP pyrophosphatase"
                     /protein_id="HIR3240937.1"
                     /translation="MKPVKAWMAWSSGKDSAWALHAARRQRDITVVGLLTTVTEAYGR
                     IAMHGVREELLHAQAAALGLPLHVARIPAPCPNEAYEAAMRDALKTARRHGVTRMVFG
                     DLFLEDIRAYRERQLARIGMSGVFPLWGRKTPALAREMIHGGLQAYLVCLDPRKTPRR
                     FAGRPFDASLLADLPDNVDPCGEHGEFHTFAWNGPMFSRPVDVTIGETVEREGFVFTD
                     LVPSPRRVTCSRDETPTVDRDRRRPLARGRPRAD"
     gene            2622..3539
                     /locus_tag="AC16BC_04640"
     CDS             2622..3539
                     /locus_tag="AC16BC_04640"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR3240938.1"
                     /translation="MENVVDRPVVLDTNLDRLGPKRRGKVRDIYDLGDYLLLIATDRL
                     SAFDVVLPDGIPGKGYVLTQLSKFWFEWLWSMEDVLPHHLVTTEVDKFPPACRAYRDV
                     LDGRSMLVGKYNPLPVECIVRGYLAGSGWKEYRASGTVCGQPLPPGLREADRLPQPIF
                     TPSTKAASGHDENISFERMESLIGSDLAKLIRQCSLTIYHRAAARAESLGIIIADTKM
                     EFGLEPATNQPYLIDEVLTPDSSRFWPKDQYKPGSSPPSFDKQYVRDYLDSIGWNHQP
                     PAPKLPSDVIRKTSEKYAEALSRLTRPDR"
     gene            3561..4226
                     /locus_tag="AC16BC_04645"
     CDS             3561..4226
                     /locus_tag="AC16BC_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240939.1"
                     /translation="MDRSLSEEVDAFAAADGQRHVLLGFDARQNWLPMANWWPQNGPF
                     LYLLRENVERAASVDKNIWSSVCAALADLARPDDAGPLPPLWNDYERLCRHVESKEGM
                     QDRRYSMIAVTLVVDSLSPEASEWLPLLGPDAPDLGALDQGWRLMGYDVANPKLYSGL
                     TDAPYHTEREDIAALQSRWAGHLNERHLFISFDEAQGFLAVAEQRARRLAPFGVFGVW
                     ERV"
     gene            4295..4921
                     /locus_tag="AC16BC_04650"
     CDS             4295..4921
                     /locus_tag="AC16BC_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR3240940.1"
                     /translation="MLPALVAACGGRPISPAPGGHFTAVASWYGEPFHGRLTASGERY
                     DMHGLSAAHRSLPFGTRLRVTNPDSGQSSIVTVNDRGPFVRGRQLDLSYGAAKEIGLV
                     GNGVGRVAVEVLDRDVSYRKAVREEGTRGASGGSFAIQFGAFRDPDNAARLKQALELE
                     TQGVKITQVTVNGVIYHRVRLGPFPSRDEALLRARAFAEEGYETAIVR"
     gene            complement(4950..>5360)
                     /locus_tag="AC16BC_04655"
     CDS             complement(4950..>5360)
                     /locus_tag="AC16BC_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240941.1"
                     /translation="TTVASETVGTDIASMCTKCRIEREHTVASRVGEKIGKVRCKTCG
                     SLHRFIKPGTPVPAPRRKSSKVSPEETWSRRLETASSKRTTYTPAGAYQLDDVIDHSH
                     FGVGIVTMLMAKDKIQVTFKDGPKILIMGMRPAV"
BASE COUNT          875 a         1631 c         1800 g         1054 t
ORIGIN      
        1 ctcgcgtacc gactgagccc caggcccgtg cgcgcgatgg cctcgctctc cacgctgacc
       61 gtcagcacct tctacatgat cgcgcagatg gtgggcgccg gcgccttggt ggcgctgctg
      121 ctcaaggact ccggcctcag cttcgaggcg gcggtgatcg gcgtgggcgt gttgatgatc
      181 gtgtacgtgg tgttcggcgg catgctggcc accacgtggg tgcagatcat caaggccgtc
      241 ctgttgatgg gcgggaccgt gctgctgagc gtcttggtgc tggtgcacta cggattcgat
      301 ctcggcgagt tcttccgcgc cgcaagccac gtcacgtacc acgacaagag cgggcaggcg
      361 atcacgcaga attttctgga gcccggtctt cggttcaaac ccccatacgg ggcgctcgat
      421 ttgatttcgt tggggctcgc gctgattttc ggcaccgcgg ggttgcccca tatcctggtg
      481 cgtttctaca ccgtaccgga cgccaagacc gcgcgcgtca gcgtggtgtg ggccatggcg
      541 atcatcggca gcttttacat catgaccacg tttctgggat tcggtgcggc cacgatcgtg
      601 gggcgcgacg cgatcacggc gaacggcggc accaacatga gcgcgcagtt gctcgcgcaa
      661 gccttgggcg gcgacgtgtt cttcgcgttc atttcagcgg tggcgttcgc caccattctg
      721 gccgtggtgg cgggcctcac gatcagcgca tccacgtcgt tcgcgcacga tttctacacc
      781 aatgtcctgc accacggccg ggagcgcagc ccgggcgagg aagtgcgggt ggcgcgcgtg
      841 accgcctgcg tggtgggcgt gctctcgatg gcgatcgcga tcgtggtggg gccgaacacc
      901 aacgtggcgt tcctggtcgc actcgcgttc gcggtcgcgg cctcggccaa tctacccgtg
      961 atcgtgttct cgctgttctg gaaacgattc acgacgcgag gcgcggtctg ggggttggcc
     1021 acggggctga tctcttcgat tgcgctgatc ctggtcagcc cgagtgtgat gacgattgac
     1081 gcacccggcg tgaccggcgc ggcgcgccac ctgattcaag ccgcgccctt gttccccttg
     1141 gagaatcccg gcatcgcgag catcccgctg gggtttctcg gagcgatcgt cggtacgctg
     1201 ctgggccgcg aaccgcaggc cgaggccaag tacgcggagc ttctcgtgcg cgcgaacacg
     1261 gggctgggcg cggaacaacc gtccggtcgc tagttcctgg gaaccgccgg gacatctccg
     1321 caggcgccat gcccgcgcag gcccgggagg gaattcgatg gcctgcatgc ttacagggtt
     1381 gttagtctgc gcggggacga ccgcgcgcaa gtggccttcg gcgatctcga tcgacggtgg
     1441 gggtctcgtc tcgcgagcac gtgacgcgtc gtggcgacgg gacgagatcc gtaaacacga
     1501 acccctctcg ttccacggtc tcgcctatcg tgacgtccac tggtcgcgag aacatcgggc
     1561 cgttccacgc gaacgtgtgg aattcgccgt gctcgccgca gggatccacg ttgtcgggaa
     1621 ggtccgcgag gagggaagcg tcgaaggggc ggcccgcgaa tcgtctgggt gttttcctgg
     1681 ggtccaagca gaccagatac gcttgaagcc cgccgtgaat catctctcga gccaaggcgg
     1741 gtgtcttgcg tccccacaag ggaaacaccc ccgacatgcc gatccgtgca agctggcgtt
     1801 cacggtaggc gcggatgtcc tccagaaaca ggtcgccgaa caccatgcgc gtcacgccgt
     1861 ggcgtcgggc agtcttcagg gcatctctca tcgcggcttc gtaggcctcg ttggggcatg
     1921 gagctgggat gcgtgcgacg tggagcggaa gtcccaacgc ggcggcttgg gcgtggagca
     1981 attcctcgcg tacgccgtgc atggcgatcc gaccatacgc ttcggtgacg gtggtcagta
     2041 gaccgacgac cgtgatgtcc cgttgtcgcc gcgccgcgtg taacgcccac gcgctgtcct
     2101 tgccgctact ccatgccatc catgctttga ccggtttcat gcgaacctca ccgcgcacag
     2161 aacgccggac gataacgagg tgaatccgcg gtgtcaacgg cccgtttgtt acggcccagt
     2221 tcgggctcgt cactttcgac gaattcacca gtgctgtgga cggccgtgtt gctacgtgcc
     2281 cgtttagcca tttaggagtg tgttggccct cagtcatctg atctcattga atacacgtat
     2341 atttttctgg tcaaaaagtg agcatttcgc tcgctccagt atcaattcag ggtgattcgt
     2401 ggcaatccct ggagttgtcc ccatgttttc cacaatcatc gtggatgggc gctgcgtctt
     2461 cgttgcacgc cagcgtggct gtgatgacgt gctctctcgt gacgttggcc atgacgtgat
     2521 ccacgtcgag tcagcgttca tgcgctgggt ggttggggcg gggattgcgc ggcgggtcct
     2581 gcgtctggta tagctagcac gttcggcaat cgaggatacg catggagaac gtggtggatc
     2641 gaccggtggt gttggacacg aacttggatc gcctgggccc gaagcgccgg ggcaaggtgc
     2701 gggatatcta cgatctggga gactatctgc tgctgatcgc gacggatcgg ctgtccgcgt
     2761 tcgacgtggt gcttcctgac ggcattcccg gcaaggggta tgtgcttacc caactctcca
     2821 agttctggtt tgagtggctg tggtcgatgg aagacgtact gccgcatcat ctggtcacca
     2881 ccgaggtgga caaattcccg cccgcatgcc gggcgtaccg tgacgtgctc gatggccgct
     2941 cgatgctggt cggcaagtac aatccgcttc cggtggagtg tatcgtccgc ggctatctcg
     3001 cgggatcggg atggaaggag taccgcgcaa gcgggacggt ctgcggccag ccgctgccgc
     3061 cgggtctcag agaggccgac cggttgcccc agcccatttt cacgccgtcg accaaggccg
     3121 ccagcggcca cgacgagaac atttccttcg aacgcatgga atcgctgatc ggctctgacc
     3181 tcgcgaagct catccggcaa tgcagcctca cgatctacca ccgcgccgcg gcacgcgccg
     3241 agagtttggg catcatcatc gcggacacca agatggagtt cggcttggaa cccgccacga
     3301 accagcccta cttgatcgac gaggtgctca cgccggactc atcgcggttc tggcccaagg
     3361 atcagtacaa gcccgggagt tctcctccca gtttcgacaa acaatacgtg cgcgactatc
     3421 tggactcgat cggctggaac caccagccgc ccgcgcccaa gcttccatcc gacgtgattc
     3481 gcaagacgag tgaaaagtac gccgaggcgc tctcgcggct cacgcggccg gatcgataac
     3541 gacgtgaagg agtcaatgtg atggaccgta gtctaagcga agaagtcgac gcgtttgccg
     3601 cggccgacgg ccagcgccat gtgctcttgg gattcgacgc gcgacagaac tggctgccca
     3661 tggccaactg gtggccccag aacgggccgt ttctctacct gctgcgggag aacgtggagc
     3721 gggccgcctc ggtggacaag aacatctgga gttcggtgtg cgcggcgctc gcggatctgg
     3781 cgcgtccgga tgacgcggga ccgctgccgc cgctgtggaa cgactatgag cggctgtgcc
     3841 gtcacgtgga gtcaaaggag gggatgcagg accggcgcta cagcatgatc gcggtgacgc
     3901 tcgtggtgga ctcgctcagc cccgaggcgt ccgagtggct gccgttgctc gggccggacg
     3961 cgcctgacct gggtgcgctg gaccaaggtt ggcggctgat ggggtacgac gtcgccaacc
     4021 ccaagttata cagcgggctg accgacgcgc cataccacac cgaacgggag gatatcgccg
     4081 ccctgcaatc ccgctgggcg ggccatctca acgagcggca tttgttcatc agcttcgacg
     4141 aagcccaggg gtttctcgcc gtcgccgaac aacgggcgcg acgcctggcg ccattcggcg
     4201 tgttcggcgt gtgggagcgg gtataaggga cgccaggcca cggtccgtcg cggctggttg
     4261 ccgcagggtt gtcctgcggc tcgctgtctg cgtcatgctt cccgcgctgg tggccgcctg
     4321 cggcggccga ccgatcagcc ccgcgccagg cggccacttc acggccgtgg cgtcctggta
     4381 cggagaaccg tttcacggcc ggctcacggc ctcgggcgag cgctacgaca tgcacggctt
     4441 gagcgccgcc caccgctcgt tgccgttcgg cacgcggctg agggtgacca atcccgacag
     4501 cggtcagtcc agtattgtca cggtgaacga tcgggggccg ttcgtgcgcg gccggcagtt
     4561 ggatttatcc tacggcgcgg cgaaggagat cggtctggtg ggcaacggcg tggggcgtgt
     4621 ggcggtcgag gtccttgatc gcgatgtcag ttaccgcaag gcggtgcggg aggagggtac
     4681 gcggggcgcg tccggcggtt cgttcgcgat tcagttcggc gcgttccgcg acccggacaa
     4741 tgccgcgcga ctcaaacaag cgttggaatt ggagacgcag ggcgtaaaga tcacgcaagt
     4801 gacggttaac ggcgtgatct accaccgcgt ccgcctcgga ccatttccct cgcgagacga
     4861 ggcgttgttg cgggcccgcg cattcgcgga ggaagggtac gagacggcga tcgtgcgttg
     4921 agtcgggtgt ccggctctga gcgaccggct cagaccgccg gccgcatgcc catgatgagg
     4981 atcttcggac cgtctttgaa cgtgacctgg atcttgtcct tggccatcag catggtcacg
     5041 atacccacgc cgaaatggct gtggtcgatc acgtcgtcga gttggtacgc gcccgccggc
     5101 gtgtaggtag tgcgcttgga cgacgcagtc tccagtcgtc ggctccaggt ctcttccgga
     5161 gacaccttgg atgacttgcg gcggggggcc gggaccgggg ttccgggttt gataaagcgg
     5221 tgaagactgc cgcacgtctt gcagcgaact ttcccaatct tttcaccgac tcgggatgcg
     5281 acggtgtgtt ctcgttcgat ccggcacttg gtgcacatcg aggcgatatc agtgcccacg
     5341 gtctcgctgg ctacggtggt
//
LOCUS       DBNGTT010000207         3277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1703006, whole genome shotgun sequence.
ACCESSION   DBNGTT010000207 DBNGTT010000000
VERSION     DBNGTT010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1703006"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..822
                     /locus_tag="AC16BC_04660"
     CDS             403..822
                     /locus_tag="AC16BC_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3240942.1"
                     /translation="MTDFFGLVHSGSRGRVLVVDDEADVRKAVNLALTKSGYDVVEAD
                     DGKRAVEVINSDDNALCVDVVICDIRMPRVNGVEAIAYFRREFPTLPVIVLTGFPDVQ
                     LATSLLKQGVADYVVKPVERDTLAAAVEAAMKHYSRV"
     gene            827..1942
                     /locus_tag="AC16BC_04665"
     CDS             827..1942
                     /locus_tag="AC16BC_04665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3240943.1"
                     /translation="MDGSPSAVRSEHTTGEWSDTLAAAASGARVMERDPITVALLGAG
                     RGGSALLELFARTPGVSVSVVADKAPLAPGLRRARELRIPTTDSAADLIARHEAELIV
                     DVTGDPGLGHWLADHHPPGTDILGGTGAKLLWNLVQYEAETQAQLGQAEKLATIGTFT
                     SGVAHDINNPLYLLMSLAESLSDQPDPAIVREHARDMLRAVRRIAGIVQGLTRYARVP
                     QGDDWVHVELTGKLDEAVQLAQYAAGLNDIAVIKEYADRSAVRGRPEELLQVFVNVIT
                     NAMQAMQGRGTLRVATYRENGSVAVDISDSGPGIPQDIIEKIFDPFFTTKKPGEGTGL
                     GLHISRTIVNKFGGHIIVSSQEGHGTTFQLQFPVEAH"
     gene            1970..>3277
                     /locus_tag="AC16BC_04670"
     CDS             1970..>3277
                     /locus_tag="AC16BC_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HIR3240944.1"
                     /translation="MERRLARTRTRWGLKGRLVSLTLVVGTVPLVLGIGTAYLQGTRE
                     LHATIGASFAGLATESARKLDLVFDDELARLTQVAQDRVIVDALIERASDASTDQDEG
                     TRWTARDATLVRRITEGPVAARLMQYLPAQAPGQPVAQALLVTDASGRLVASTSAAVR
                     YASDNETWWRKAMANGEGHAYVGNVAFDSALNTYVFHLSTPVAKGRRDRPVGVVHRVF
                     DAKAYFAPYVSPIRFGKTGHVMLIDSAGTVMSCPVLPTGTQLADAELVSLVTPPYAGW
                     DNTPSDGHGGHASSLVGFSPLTDTNRFTLASTGTSWHTFAWQSTDELFAPTRRLLAWV
                     AGIGLAAIGLLGTLGYVAAIRIVRPIRSLQQGAALIGRNELKEPIVVSTGDEIQGLAE
                     EINRMNSRLQDAFSGLNQEVRSLQAYNEQILNSVPNPIIMLDHE"
BASE COUNT          593 a         1066 c         1067 g          551 t
ORIGIN      
        1 ccgaacagga acttcaggtc acccttgaga tgggcgtggc gctggtccag gggtttttgt
       61 tcgggaagcc cggcgatctt gattcgggtt ctctccgtcc cccggtcccc aagagcgcgg
      121 cgtaagacag aaaccgctcc acacaaccat ccaggcaaga tcttgccctc aaaccttgat
      181 gggactggtg cggtttgcgc ggtgtgagca caagacccga gccagtcatg gctctttctc
      241 agcaaccatt atggttttga ttgaggttca gccatcaacg aagtttttct tgacttgtca
      301 tttcttgtgg tgcctataat cgcgcgtcgg taccttggga tacctaggga aggggcaagg
      361 ttgtccagcc cgcgtcagag atgacgcgaa aggaggggct ccatgacaga cttcttcggg
      421 ttagtccact caggcagccg cggtcgggtg ttggtcgtgg acgacgaggc cgatgtccgc
      481 aaggccgtca atctcgcgct caccaaatcc ggatatgatg tggttgaggc cgacgacggg
      541 aaaagagccg tcgaggtcat caattccgac gacaacgccc tgtgcgtcga cgtcgtgatc
      601 tgcgacatcc gtatgccccg ggtcaacggc gtggaagcga tcgcctattt tcgccgggag
      661 ttccccacgc tccccgtcat cgtgctgacc gggttccccg acgtgcaact cgcgacctcg
      721 ctgctgaaac agggcgtcgc ggactatgtg gtcaaacccg ttgagcgcga caccctggcc
      781 gcggccgttg aagccgcgat gaagcactac agccgggtct aatgccttgg acggctcgcc
      841 ctcggccgtc cgctcggagc acaccacggg cgaatggagc gatacgctgg cagccgcggc
      901 ctccggggcg cgcgtgatgg agcgcgaccc gatcaccgtg gccctgcttg gcgcgggccg
      961 tggcggctcc gcgctcctgg agttgttcgc cagaaccccg ggcgtctcgg tgagcgtggt
     1021 cgcggacaaa gcccccttgg cgcccggatt gcgccgcgcc cgcgagctcc ggattcccac
     1081 caccgacagc gcggccgacc tgatagctcg ccacgaggcc gagctgatcg ttgacgtgac
     1141 cggcgatccc ggcctggggc actggctcgc ggatcaccat ccgccgggca ccgacatcct
     1201 gggcggcacc ggcgccaagc tgttgtggaa tctggtccag tacgaagccg agacccaggc
     1261 gcaactgggg caagccgaga agctggccac gatcggaacc ttcacctccg gcgtggcgca
     1321 cgacatcaac aatccgctct acttgctcat gagcctggcg gagagcctct cggatcaacc
     1381 ggacccggcg atcgtgcgcg aacacgcgcg cgacatgctg cgggcggtgc ggcgaatcgc
     1441 gggcattgtt caaggcttga cgcggtatgc ccgcgtgccg cagggggacg attgggtgca
     1501 cgtcgagctc accggaaagt tggacgaggc cgtccagctg gcgcaatacg cggccgggct
     1561 caacgacatc gcggtcatca aggagtacgc ggatcggtcc gcggttcggg gacggcccga
     1621 ggaactcctg caggtcttcg tgaacgtgat caccaacgcg atgcaggcga tgcaggggcg
     1681 gggcaccttg cgggtggcca cgtatcgcga gaacggctcg gtcgcggtgg acatcagcga
     1741 ctccggaccc gggattccgc aggacattat tgaaaagatc ttcgacccct tctttaccac
     1801 caaaaagccc ggtgaaggga ccggcttggg actccacatc tctcgaacca tcgtcaacaa
     1861 gttcggcggg cacatcatcg tctcgagtca ggaagggcac ggtacgacct ttcaattaca
     1921 gttcccggtc gaagcgcact aaggtcctct cacaccggga gtgtcgctca tggaacgtcg
     1981 gttggccagg acccgcacgc gctggggact gaaggggcga ctcgtgtcgt tgaccttggt
     2041 cgtcggcacg gtccccctcg tgctcggcat cggcacggcc tacctgcagg gcacccgcga
     2101 attgcacgcg accatcgggg ccagcttcgc ggggctggcc acggagagcg cgcggaagct
     2161 cgacctggtc ttcgacgacg aactggcccg gctcacccag gtcgcgcagg accgggtgat
     2221 cgtcgacgcg ctgatcgagc gcgcaagcga cgcgagcacg gaccaggatg aaggaacgcg
     2281 gtggacggcg cgcgatgcaa cgctcgtgcg acgcatcacc gagggccccg tcgccgcccg
     2341 cctgatgcag tatctccccg cccaggcccc cgggcaaccc gtggcgcagg cgctgctcgt
     2401 caccgacgca tccggccgac tcgtcgccag caccagcgcc gcggtccgat atgcctccga
     2461 caacgaaacg tggtggcgca aggcgatggc caacggggag gggcacgcgt acgtcggcaa
     2521 cgtcgctttc gactccgcgt tgaacacgta cgtgtttcac ctgtccactc cggtcgcgaa
     2581 aggccgacgc gatcggccgg tcggcgtggt gcaccgggta ttcgacgcca aggcgtattt
     2641 cgccccgtat gtgtcaccga tccggttcgg aaaaaccgga cacgtcatgc tgatcgacag
     2701 cgcgggcacg gtgatgagct gcccggtgct gccgaccggc acgcagctgg ccgacgccga
     2761 gctggtgtcc ctggtgaccc cgccctacgc cgggtgggac aacaccccca gcgacggtca
     2821 cggcggccac gcctcgtcgc tggtgggatt ctccccgctg accgacacca atcgtttcac
     2881 gctcgcctcc accgggacaa gctggcacac gttcgcgtgg cagtcgacgg acgagttgtt
     2941 cgcgccgacg cgccggttgc tggcgtgggt cgcgggcatc gggctggccg ccatcggcct
     3001 gctcggcacc ctcggatacg tcgccgcgat tcgcatcgtg cgacccatcc ggagcctgca
     3061 acagggcgcc gcgttgatcg gtcggaacga attgaaggag ccgatcgtgg tcagcaccgg
     3121 cgacgagatc cagggactgg ccgaggagat caaccggatg aacagccggt tgcaggacgc
     3181 cttttccggc ctgaaccagg aagtgcgttc cctccaagcg tacaacgagc agattctgaa
     3241 cagcgtgccc aacccgatca tcatgctgga ccacgaa
//
LOCUS       DBNGTT010000208         4741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1319310, whole genome shotgun sequence.
ACCESSION   DBNGTT010000208 DBNGTT010000000
VERSION     DBNGTT010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1319310"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            501..1751
                     /gene="urtA"
                     /locus_tag="AC16BC_04675"
     CDS             501..1751
                     /gene="urtA"
                     /locus_tag="AC16BC_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HIR3240945.1"
                     /translation="MTRCGLRTIRYRLRKLVVWGVTTWFVLALAAVAPAALPTAQVNT
                     TKLAVTDTEVTVGQLHSATGTMAISETGSIQAEQLAIDQINAMGGVLGRKIKVIQEDG
                     ASDWPTFAEKAKKLLVSDRVAAVFGCWTSASRKAVLPVFEKENGMLYYPTFYEGLEQS
                     KNVIYTGQEATQQIIAGLDWIVKEKGAKTFFLIGSDYIWPRTSNKIARKHIENVLKLK
                     VVGEEYYPLGHTQFGALINKIKLQKPDVVYAIVVGGSNVAFYKQLKAAGVTSAKQTLL
                     TISVTEDEMLGIGGENVEGFYAAMKYFQSLDNPNNKKFVEAFKAKYGPKSVIGDVTQA
                     AYLGPWLWKAAVEKAGSFDIDKIAAASPGTELSTAPEGYVKLHPNHHLWSKLRIGQAQ
                     KDGQFKVLYESALIEPDPFPKGYQ"
     gene            1801..2715
                     /gene="urtB"
                     /locus_tag="AC16BC_04680"
     CDS             1801..2715
                     /gene="urtB"
                     /locus_tag="AC16BC_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HIR3240946.1"
                     /translation="MSLTDLAPILAMQAFNGISVFCVLLLMALGLAIIFGQMGVINMA
                     HGEFMTLGAYMTYVCSTLTTSYFPAFLPYYFVVAIVSAFVMAFLFGYAVERLLIHQLY
                     RRPLDTLLATWGLSLGMQQAFRSTFGAREVTATLPDWLMGSVQPTPNIDIPINGLFVM
                     GLTVVLTGGLFLLLFRSRWGLHVRATVQNRVMSGAVGINTKRADRLTFSLGCGIAGIA
                     GAAFTTIGSTGPTSGSLYIVDTFLTVVFGGASSLFGTIASAFLIGQTQSISEFFMTGS
                     MAKVLTLTAVVVILMMRPQGLFAAKLRK"
     gene            2738..3907
                     /gene="urtC"
                     /locus_tag="AC16BC_04685"
     CDS             2738..3907
                     /gene="urtC"
                     /locus_tag="AC16BC_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HIR3240947.1"
                     /translation="MKAIYDNVFGGKEGLMYLGALAVIVFVVFPLTLDLFRLSMVGKY
                     LTYAFVAVGLVLCWGYGGILSLGQGVFFGLGGYCMAMFLKLEASDPESTKIQSTPGIP
                     DFMDWNQLTALPWFWEPFKSFPFTLFAVLAVPGLLAVILGLAMFKRRVGDVYFSIVTQ
                     AVALILTVLIIGRQGYTGGVNGITDLKTLLGWDIRTDSAKYLLYYLNGALLIGCILVS
                     RFILSSKLGKLLLAMRDKEQRVRFSGYDVANFKVFIFTVGSLFAAIGGAMFALQVGFM
                     SPSFVGIIPSIEMVIFAAVGGRFSLLGAVYGTLLVNYGKTLFSETFPQLWLFLMAALF
                     ISVVMVFPDGLAGVYDKLEKRWRAHRLAIRERLAVELAAARRRMSGPKAPGGMHA"
     gene            3904..4647
                     /gene="urtD"
                     /locus_tag="AC16BC_04690"
     CDS             3904..4647
                     /gene="urtD"
                     /locus_tag="AC16BC_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HIR3240948.1"
                     /translation="MNNTDFALSIEGLTVSFDGFKAVDGVNLYVDTNELRVVIGPNGA
                     GKTTLLDLICGCTKATGGSIKFKGRELTRLSEHAIVRAGVGRKFQTPSIYENLTVFEN
                     LEVSFPRGRHVFSALTFRRTPDVKERVEQVAAEIFLADRLHDQADLLSHGQKQWLEIG
                     MLLMQDPELLMLDEPVAGMSASEREKTATLLNRIAKNRSVIVIEHDMEFVKNIAHRVT
                     VLHQGKVLAEGSMDAIHSNPQVVEVYLGH"
BASE COUNT          932 a         1355 c         1515 g          939 t
ORIGIN      
        1 ggcggggcca ttcagaaacg agctccagca atgttggact gcaagattcg gccctaacct
       61 ggattcaggg gtctaaggtt agacagaccg ctgcaccacg cgaaaagcgg ggtgggccgg
      121 gagcagaaaa cgatgccatg gccgagcagc gaaacatgag ccttagccaa caacaaaagt
      181 gtatggcttc aggcacatta ctgtttgacg gtcaacaatt gctcgctctc cttccgctaa
      241 gaatccccaa tacaggcgcc tgataatacc tttagagtat taccgttcgg cacgctattc
      301 gcattagggt caggcaaggc catgcagagg gatggacgac tgaccgagat agagaaggca
      361 tttggtcgcc cttcgggcgg cgatgccttc ccacctgccg taccctcacg gcacgcaacg
      421 gtatgcacgc atgctcctct cgtctctaca cattgatgag atgctgagat cctgcgtcca
      481 tacggcacaa ggagcaatag atgactagat gtgggctcag aacgattcgg tatcggcttc
      541 gcaagctggt ggtatggggc gtgacgactt ggtttgtgtt agcgctggcc gctgttgcgc
      601 ctgccgcgct gccaactgcg caggtgaaca ccacgaagct cgccgtcacc gacacagaag
      661 tcacagtcgg acaattgcat tcggccaccg gcaccatggc gatcagcgaa accgggtcga
      721 tccaggccga acaactcgcg atcgatcaga tcaacgccat gggcggcgtg ttggggcgaa
      781 agatcaaggt gatccaggag gacggcgcga gcgattggcc gacctttgcc gagaaggcga
      841 aaaagttgct ggtcagcgac cgggtggccg cggtgttcgg gtgttggacc tcggcctcgc
      901 gcaaggcggt gttgccggtg ttcgagaaag agaacgggat gctctactac ccgacgttct
      961 acgaaggact cgaacaatcg aaaaacgtga tctacacagg ccaggaagcc acgcagcaaa
     1021 tcatcgcggg cctcgactgg atcgtgaaag agaagggcgc caagacgttt ttcctgatcg
     1081 ggtcggacta catctggccg cggacgtcca acaagatcgc gcgcaagcac atcgagaacg
     1141 tcctgaaatt gaaagtggtg ggtgaggagt actacccgct gggccacacg cagttcggcg
     1201 cgttgatcaa caagatcaag ctgcaaaaac ccgacgtcgt gtacgcgatt gtggtgggcg
     1261 gcagcaacgt ggcgttctac aaacagttga aggccgcggg cgtcacctcc gccaaacaga
     1321 cgctgctgac catttcggtg accgaggacg agatgttggg gatcggagga gaaaacgtcg
     1381 aagggttcta cgccgcgatg aagtatttcc agagcctgga caatcccaac aataagaaat
     1441 tcgtggaggc gttcaaagcc aaatacgggc cgaagtccgt gatcggcgac gtgacgcagg
     1501 ccgcgtatct cgggccctgg ttgtggaagg ccgcggtgga aaaggccgga agtttcgaca
     1561 tcgacaaaat cgcagccgcg tcgccgggca ctgaattgag caccgcaccg gaaggctacg
     1621 tgaagctgca tccgaaccac cacctgtgga gcaagttgcg catcggtcag gcgcagaagg
     1681 acggacagtt caaggtgctc tacgagtccg cgttgatcga gccggacccg ttccccaagg
     1741 gctatcaata acgccggcgg ccccgggcaa ccatgagacc acggcaaagg gagacgcggg
     1801 atgtcactga ctgacttggc tccaatcttg gccatgcagg cgttcaacgg aatctccgtg
     1861 ttctgcgtgc tgctgctgat ggcgctgggg ctggcgatca tcttcgggca gatgggcgtg
     1921 atcaacatgg cgcacggcga gtttatgacc ctgggcgcct atatgaccta cgtgtgctcg
     1981 acgctcacca caagttactt cccggcgttc ctgccgtact acttcgtcgt ggcgatcgtg
     2041 agcgcgttcg tcatggcgtt tctcttcggg tacgcggtcg aacggttgtt gatccaccaa
     2101 ctgtatcgtc gaccgctcga caccctgctc gcgacgtggg gtttgtcgct gggcatgcag
     2161 caggcgtttc ggtccacgtt cggcgcccgc gaagtcacgg cgacgctccc ggactggctg
     2221 atggggtcgg tccagcccac gcccaacatt gacatcccga tcaacggcct gttcgtgatg
     2281 gggttgacgg tggtgctcac gggcgggttg ttcctgctgt tgttccgatc ccggtggggg
     2341 ctccatgtgc gcgccaccgt gcaaaaccgg gtcatgagcg gggcggtcgg gatcaacacc
     2401 aaacgggcgg accgcttgac cttctcgctg gggtgcggca tcgcgggaat cgcaggcgcg
     2461 gcgttcacca caatcggctc gaccggcccc accagcgggt cgctctacat cgtggacacg
     2521 ttcctcaccg tggtgttcgg cggcgcgagc agcctctttg gcacgatcgc gtcggcgttc
     2581 ctaatcggcc agacgcagtc gatctccgaa ttcttcatga ccgggtccat ggccaaggtc
     2641 ctcaccctga ccgccgtggt cgtgattttg atgatgcggc cgcagggcct gttcgcggcc
     2701 aaattgcgca aatgaccggg aggcgcgagg cgtcacgatg aaagcgatct acgacaacgt
     2761 gttcggcggc aaagagggcc tgatgtacct cggagcgctc gccgtgattg tgttcgtggt
     2821 gttccccctg acccttgacc tcttccgcct gagtatggtc ggcaaatacc tgacctacgc
     2881 gttcgtggcg gtggggctgg tgttgtgttg gggctacggc ggcattctca gcttggggca
     2941 gggtgtgttc ttcggactcg gcggctattg catggccatg ttcctcaagc tggaggcgtc
     3001 cgatccggag agcaccaaga ttcagtccac gccggggatc ccggatttca tggattggaa
     3061 ccaactcacc gcgttgccgt ggttttggga accgttcaag agtttcccgt tcacgttgtt
     3121 cgcggtgctg gcggtgccgg gtctgctggc ggtgatccta ggcctggcga tgttcaagcg
     3181 tcgggtgggc gacgtgtact tctccattgt cacgcaagcc gtcgcgctga tcttaaccgt
     3241 gctgatcatc ggccgccagg ggtacaccgg cggggtcaac gggatcaccg acctcaagac
     3301 cctgctgggc tgggacatcc gtaccgattc cgccaaatac ctgctctact atctcaacgg
     3361 ggcgctgttg atcggctgca tcctggtttc acgcttcatc ctgtcgtcca agctcggcaa
     3421 attgctgctg gccatgcgcg acaaggagca gcgcgtgcgc ttttccggct acgacgtcgc
     3481 caacttcaag gtgttcatct tcaccgtcgg ctcgctgttc gcggcgatcg gcggggcgat
     3541 gttcgcgctg caggtggggt tcatgtcgcc gtcgttcgtg ggcatcatcc cttccatcga
     3601 aatggtgatt ttcgccgcgg tgggcggtcg gttctcgctg ctcggcgcgg tctacggcac
     3661 gttgctggtc aactatggca aaaccttgtt ctcggaaacg tttcctcagt tgtggctgtt
     3721 cctgatggcg gcgttgttca tttcggtggt catggtgttt ccggacggtc tcgcgggcgt
     3781 gtacgacaaa ctcgagaagc gctggcgcgc ccatcggctg gcgatccggg aacggctggc
     3841 cgtggagctc gcggccgcgc gccgacggat gtccggacca aaagcgcccg gagggatgca
     3901 cgcatgaaca atacggattt cgcgctgtcg atcgaagggc tgacggtgtc gttcgacgga
     3961 ttcaaagccg ttgacggcgt caacctctac gtcgacacga acgagttgcg cgtggtgatc
     4021 ggcccgaacg gcgccggcaa aaccacgcta ctcgacctga tttgcgggtg caccaaggcc
     4081 acgggcggca gcatcaagtt caagggccgc gagctcacgc ggctgagcga gcacgcgatc
     4141 gttcgcgccg gggtgggacg gaaattccag acgccctcga tctacgagaa cctcaccgtg
     4201 ttcgagaacc tggaggtctc gtttccgcgc ggccggcacg tgttcagcgc gttgaccttc
     4261 cggcgcacgc cggacgtgaa ggaacgggtc gagcaggtgg ccgcggagat cttcctggcc
     4321 gaccgcctgc acgaccaggc cgacctgctc tcgcacgggc agaagcagtg gctcgaaatc
     4381 ggcatgttgc tgatgcagga cccggagttg ttgatgctcg acgagccggt ggcgggcatg
     4441 agcgcctcgg agcgcgaaaa aaccgcgacg ttgctcaacc gcatcgcgaa aaaccgctcc
     4501 gtgatcgtga tcgaacacga catggagttc gtcaagaaca tcgcgcatcg ggtgaccgtg
     4561 ctgcaccagg gcaaggttct ggcggagggc agcatggacg cgatccactc gaatccgcag
     4621 gtcgtcgaag tctatttagg ccactaacgg ggacggaacg cgcatgttga aagtcagcaa
     4681 tctggtgtcg ggatacggac agagcgaagt gattcacgac gtgagtttct ccgcggatcc
     4741 t
//
LOCUS       DBNGTT010000209         1523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_553770, whole genome shotgun sequence.
ACCESSION   DBNGTT010000209 DBNGTT010000000
VERSION     DBNGTT010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_553770"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="AC16BC_04695"
     CDS             complement(<1..583)
                     /locus_tag="AC16BC_04695"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3240949.1"
                     /translation="MTGITRRIHRGAEERAGTGSGATARRPPIGKLIVVAAVACAVAA
                     FITFDLGRYLTLASLKSNKDALRTYTDSHYAASVSIFIIAYCAQTALSLPGAVIFTLT
                     GGFLFGTWLGTLYVNIGATSGAVLAFLVARYLFREGVERRFGAKLETIQRGFSRNAFN
                     YLLTLRLIPLFPFFLVNLASGLTRIRLSTYTAAT"
     gene            complement(580..>1523)
                     /locus_tag="AC16BC_04700"
     CDS             complement(580..>1523)
                     /locus_tag="AC16BC_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR3240950.1"
                     /translation="GDGVEYNTVDAALWYIEAIRAYYAATLDDATLGALFPVVEDIVA
                     CYTKGTRFGIGLDVRDGLLTAGEPGVQLTWMDAKVGDRVITPRIGKPVEVNALWYNAI
                     RTAAAFARRLRRDPRVYAEMADRTRDGFSRFWNSDNACCDDVVDGPDGRDASVRPNQL
                     FAVSLPESPLTNDQQRGVVDACARKLLTSHGLRSLAPDDPRYLGRYEGGVGARDAAYH
                     QGTVWGWLLGPFALAHLRVHRDPAAAAAFLEPMARHLTAAGVGSLSEVFDGDPPFTPR
                     GCIAQAWTVAEVLRAWSEISQFQSPGRRRATPARTSR"
BASE COUNT          291 a          526 c          466 g          240 t
ORIGIN      
        1 cggtcgcagc cgtgtacgtc gagaggcgaa tgcgcgtcag ccccgacgcg aggttgacca
       61 gaaagaacgg gaacaacgga atcaaccgca gcgtcaataa ataattgaac gcgttccgac
      121 tgaatccccg ttgaatcgtt tccaacttgg cgccgaatcg acgctccacc ccctcccgga
      181 acagataccg cgcgaccagg aacgcaagca ccgcgcccga ggtcgccccg atgttcacgt
      241 acagcgtccc caaccaggtc ccgaacagga acccaccggt cagcgtgaag ataaccgcgc
      301 ctggcaagga caacgcggtc tgcgcgcaat aggcgatgat aaagatgctg accgaggcgg
      361 cgtagtgcga atcggtgtac gtccgcaacg cgtccttatt ggacttcagc gacgccagcg
      421 tcagatatcg tccgagatca aacgtgatga acgccgccac cgcgcaggcc acggccgcca
      481 ccacgatgag tttgccgatc ggcggacggc gggcggttgc ccccgacccg gtgcccgccc
      541 gttcctctgc cccacggtga atgcgccgag tgatacccgt cacctcgacg ttcgcgcggg
      601 ggttgcgcgg cgtcgtccag gagattgaaa ttggctgatc tcgctccatg cccggagcac
      661 ttcggccacg gtccacgctt gcgcgatgca accgcgcggt gtgaacgggg gatcgccgtc
      721 gaagacttcg ctcaagctcc ccactcccgc ggcagtcagg tggcgggcca tcggctccaa
      781 gaacgcggcc gcggctgcgg gatcgcgatg cacccgtaga tgcgccagtg cgaacggccc
      841 cagcagccag ccccacaccg tgccctgatg ataggccgca tcccgagcgc ccacgccgcc
      901 ctcgtaacgg ccgagatacc gcgggtcgtc cggcgccaga ctccgcaggc cgtgggatgt
      961 gagcagtttc cgggcgcatg catcgaccac cccgcgttgc tgatcgttgg tcagggggct
     1021 ctcgggcaac gaaaccgcga acagctgatt gggacgaacc gatgcgtcgc ggccgtcggg
     1081 gccgtccacc acgtcgtcac aacaggcgtt atcggagttc cagaaccgcg agaatccgtc
     1141 ccgtgtccga tcggccattt cggcatacac tcgcgggtcg cgacgcaacc ggcgcgcgaa
     1201 cgcggccgcc gtacggattg cgttgtacca taacgcgttg acctccaccg gcttgccgat
     1261 gcggggcgtg atgacgcggt cgccgacctt tgcgtccatc cacgtgagct gcacgcccgg
     1321 ttctcccgcg gtcagcagcc cgtctctgac atccaggccg atcccaaacc gcgtgccctt
     1381 cgtatagcaa gccacgatgt cttccacgac gggaaatagc gcacccagcg tcgcgtcgtc
     1441 gagagtggcc gcatagtagg cacggatcgc ctcgatgtac cacagtgccg cgtccaccgt
     1501 gttgtattcg acgccgtcac cgg
//
LOCUS       DBNGTT010000210         4827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2981641, whole genome shotgun sequence.
ACCESSION   DBNGTT010000210 DBNGTT010000000
VERSION     DBNGTT010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2981641"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="AC16BC_04705"
     CDS             complement(<1..381)
                     /locus_tag="AC16BC_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240951.1"
                     /translation="MRQAVEQDAEQDADGEATRSGSGSLETRVARVAEPIAAQLGVSL
                     VSVRYVGSRTAGTLRLTIDKPGGVTLDDCTRLSRAVGHALDVEEPIEHRYTLEVSSPG
                     LDRALEGRRDFEDAVGRLVRIKTRA"
     gene            complement(533..1417)
                     /gene="dat"
                     /locus_tag="AC16BC_04710"
     CDS             complement(533..1417)
                     /gene="dat"
                     /locus_tag="AC16BC_04710"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018873409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HIR3240952.1"
                     /translation="MNGRFLPIAKAKVSVEDRGFQFGDGVYEVVRSYNGRLFRLDAHL
                     ERLAQSAAAIRLPLRYSRAQWRRLILRAYGLSKFPEAKVYLQVTRGVAPRDHRFPKGV
                     RPTAVITVRKLDALPEAARRDGVRVITTPDLRWARCDVKSLNLLANVLAREEARAAGV
                     FEAILVRDGHVTEGSVSNVFAVINGAVTTSPTDPSILPGITRAATLELIRREGIPLVE
                     RAVGVEELRGADEVFLTGTTVEIVPVVSVDGAAVGKGIPGPVTQRLAQAFAAMVRAEC
                     ASNAAPARRRVAGRKRMR"
     gene            complement(1438..2148)
                     /locus_tag="AC16BC_04715"
     CDS             complement(1438..2148)
                     /locus_tag="AC16BC_04715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3240953.1"
                     /translation="MGVSGDGIYRAQRGYFRRAYETGRHGWPTVGVSPMVARFLRSAK
                     TRGGRALDIGCGEGRHSVAFAQRGFDAVGVDLEPKALARARAAAKGAGARPTFMQANV
                     FALPFAPDAFDVVMDYGCLHHVRRADTRRYLDQVTPLVKPGGYFLLSCFSTKFKHHPD
                     ERRTRNWLVHAGHYDRFFRREDFRPLFGRWFDIIELTEDRDGSYVFHNVLMRKRGTSD
                     GRPSRGAKRSGRSARRRA"
     gene            2243..2899
                     /locus_tag="AC16BC_04720"
     CDS             2243..2899
                     /locus_tag="AC16BC_04720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3240954.1"
                     /translation="MPSVVILDCDGTLVDSAGHIAAVFNEAIVTLGYAPLPPERVARI
                     IGLSFDDALHHLLPDSSADERTRIIHAYRARYAVDAAGRDPLFAGVRDFLDVVRSQGR
                     HLAMATGKSRAGALRIIAEHGLGDYFMAVKTGDLCRPKPHPEMLFQILDEMGVPAQRA
                     VMVGDTTFDVEMARAAGVRAIGVGWGAHDARELVDAGADLVVPGLPDLAPYLPRLFPD
                     "
     gene            complement(2896..>4827)
                     /locus_tag="AC16BC_04725"
     CDS             complement(2896..>4827)
                     /locus_tag="AC16BC_04725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR3240955.1"
                     /translation="SAFDLFPLLKQTGDDRCLLDALSGKTVLAKDRPYAAAGTDRGFF
                     EGQYAPLFGETGSIIGGLAVFRNTTERKRAETILRKRVEQQRMAKEVGERCLEASSAD
                     EVMERATPLIAGGADADLAEILMLDGRGDTLVRVAGYGWGKHVLGPFVMPAAADSQQG
                     YTLAVNGPVVVDDLAAERRFAASSLLTEQHVVSGLSVPLVSRSRTIGVLAVYARRPRA
                     FTIDDVEFVRSVAALIAVAIDRRKAEATLEASAIHDELTGLYNRRYFHGRFDEEVARA
                     SRTHRSLAVMLCDVDRFRSINDMRGHSVGDDVLKAVAKRLQGAVRETDLVFRWGADEI
                     VVVLPEVAHDAVAVTADRIRRSVRQLGETLDEELDLSIGVVLYPDHGTTSDELMRVAD
                     RALFFAKKSGDKVHIGEADYHLNDQSIEVVFQPIMDLRTREVMGYEALGRDPSGKVGI
                     VELFKRFQAIGRLRELKRLCFETQLRTARAIGLARVFLNIEFDVLRGIDPPDAPPGME
                     VILEISEVEALHDVAEHLAIAKQWRKKGYQFAMDDFGAGFISLPFIGQLVPDYIKLDR
                     SAILQAAASEGFRAFSKDLVCALKNYVRAGFIAEGIETEAELRVAHDLGISLAQGFLL
                     GRPQPLSPGDLQTGLQRAA"
BASE COUNT          798 a         1736 c         1523 g          770 t
ORIGIN      
        1 ggcgcgggtc ttgatccgca ccaggcggcc caccgcgtct tcaaagtcac gccgtccctc
       61 aagcgcccgg tccaggcccg gcgacgacac ctccagggtg taccggtgct cgatcggttc
      121 ctccacgtcc aacgcgtgtc cgaccgcgcg gctcagccgg gtgcagtcgt ccagcgtgac
      181 gccgcccggc ttgtcgatcg tgaggcgcag ggtgccggcg gtccggcttc ccacgtagcg
      241 cacgctcacc agcgagacgc cgagttgcgc ggcgatcggc tcggccacgc gcgccacgcg
      301 cgtttcgagc gaacccgacc ccgagcgtgt cgcttcgccg tccgcgtcct gctccgcgtc
      361 ctgctccacc gcctgcctca ccatctgctc ccggtgatcc gccgaaacaa aaaaagtggg
      421 ccaaacaccg cccacttcgc accgccacgc caaaagcgtg ggacccgtcg gatgccaaag
      481 cgtcggcact ataccacatc cggttcacgt gccgcaagac aacccacacg cgtcacctca
      541 tccgcttgcg acccgcaacc cgtcgtcgcg cgggcgccgc gttcgacgcg cattctgcgc
      601 gcaccatcgc ggcaaacgcc tgggccaatc gctgcgtcac cggcccggga atcccttttc
      661 cgaccgccgc cccgtccacc gagaccaccg gcacgatctc caccgtggtg ccggtcagaa
      721 acacctcgtc ggccccgcgc aattcctcca cgccgacggc gcgctccacc aacggaatgc
      781 cttcccgccg aatcagttcg agggtggcgg cccgcgtgat ccccggcaag atggacggat
      841 cggtcggcga ggtggtgacc gcgccgttga tcaccgcgaa cacgttgctg accgaccctt
      901 cggtcacatg gccgtcgcgc accaggatcg cttcgaacac cccggccgcg cgcgcttctt
      961 cccgcgccag cacgttggcc aggagattca gtgatttgac gtcgcaacgc gcccaccgca
     1021 gatccggcgt ggtgatcacg cgcaccccgt cccgccgcgc cgcttccggc aaggcatcga
     1081 gcttgcgcac cgtgatcacc gccgtgggcc gcaccccttt gggaaaccgg tgatcgcgcg
     1141 gcgcgacccc gcgcgtcacc tgcaggtaga ccttggcctc gggaaacttg ctcaacccgt
     1201 acgcgcgcaa gatcaagcgc cgccattgcg cgcgggaata ccggagcggc aaccggatcg
     1261 cggccgcgct ctgcgcgagc cgctccaggt gggcatccag tcggaacaac cggccgttgt
     1321 aactgcggac gacttcgtag accccgtcgc cgaactgaaa gccgcggtct tcgaccgaga
     1381 ccttcgcctt cgcgatcggg agaaaccgtc cgttcacgca cgcaatcgtc ggcataatca
     1441 ggcgcgcctc cgcgcggagc ggcccgatcg tttggcgccg cgtgacggcc ggccgtcgct
     1501 cgtcccgcgc tttcgcatca gcacattgtg aaacacgtac gacccgtctc ggtcttcggt
     1561 cagctcgatg atgtcgaacc agcggccgaa cagcggccga aagtcctcgc ggcgaaaaaa
     1621 ccggtcgtag tgccccgcgt gcaccagcca gttcctggtg cggcgctcgt cggggtggtg
     1681 tttgaacttg gtcgaaaagc aggagagcag aaaatacccg ccgggcttta ccagcggcgt
     1741 cacctggtcc agataccgcc gcgtgtccgc gcgccgaacg tggtgcaggc acccatagtc
     1801 catgaccacg tcgaacgcat ccggggcaaa gggcaacgca aacacattcg cctgcatgaa
     1861 cgtcggtcgg gcgccggcgc ccttggccgc ggcgcgcgcc cgcgccaacg ccttgggttc
     1921 cagatccacc cccaccgcgt cgaagcctcg ttgcgcgaac gcgacgctgt ggcgcccctc
     1981 cccgcacccg atatcgagcg cccgcccgcc gcgcgtcttc gcggagcgga gaaatcgcgc
     2041 gaccatcggc gacacgccga ccgtgggcca cccgtgccgg cccgtttcat acgcgcggcg
     2101 aaagtaccct cgctgcgcgc gataaatccc gtcgccactc acgcccacgg gcgcgtagtc
     2161 tactctcgcg tgccgcgtga atcaagacag gtctcgcact ggcctcgacc cggccgctcg
     2221 gttatactcg ccccgtccga tgatgcccag cgtcgtcatc ctggattgcg acggtacgct
     2281 cgtggacagc gcggggcaca ttgccgcggt gttcaacgag gcgatcgtga cgctcggcta
     2341 tgcgccgttg cccccggagc gcgttgcgcg gatcatcggg ctgtcgttcg acgacgcgct
     2401 gcaccacctc ctgcccgatt cttcggcgga cgagcgcacg cgcatcattc acgcgtaccg
     2461 cgcgcggtac gcggtggacg cggccggacg cgatccgctc tttgccgggg tgcgcgactt
     2521 tctggacgtc gtgcgcagcc agggacgcca cctggccatg gccacgggca agagccgggc
     2581 cggcgcgctg cggatcatcg cggagcacgg actgggcgac tacttcatgg cggtcaagac
     2641 cggggatctg tgccgcccca aaccccaccc cgagatgttg tttcagattc tggacgagat
     2701 gggcgttccc gcgcaacggg cggtcatggt cggcgatacc acgttcgacg tggagatggc
     2761 gcgcgccgcg ggggttcgag cgatcggcgt gggctggggc gcgcatgacg cgcgggagct
     2821 ggttgatgcc ggcgcggact tggtggtgcc gggcctcccc gacctggcgc cgtacctgcc
     2881 gcgactgttt cctgattagg cggcgcgctg aagcccggtt tgcaggtcgc cgggggacaa
     2941 cggctggggc ctccccagca aaaaaccctg ggccaaggaa attcccaggt catgcgccac
     3001 gcgcagttcg gcttcggtct cgatgccctc ggcgatgaat ccggcccgca cgtagttttt
     3061 cagcgcacag accagatctt ttgaaaacgc gcgaaaaccc tcggacgcgg ccgcctggag
     3121 aatggccgac cgatcgagct tgatatagtc gggcaccagt tgcccgatga acgggagcga
     3181 gatgaacccc gcgccgaaat cgtccatggc gaactgataa cccttcttgc gccactgttt
     3241 ggcgatcgcc aggtgttccg cgacgtcgtg cagggcttcg acttcggaaa tctcgaggat
     3301 cacctccatg ccgggcggcg cgtcgggcgg gtcgataccc cgcaacacgt cgaactcgat
     3361 gttgaggaac acccgcgcca gcccgatcgc gcgcgcggtg cgaagctggg tctcgaaaca
     3421 caggcgcttc aactcgcgga gccgaccaat ggcctgaaac cgtttgaaca gttcgacaat
     3481 gccgactttt cccgaggggt cgcgacccag cgcctcgtac cccatcacct cgcgcgtgcg
     3541 caggtccatg atcggttgaa acaccacctc gatcgactgg tcgttcaaat gatagtcggc
     3601 ctcgccgatg tggaccttgt caccgctttt tttggcgaag aagagcgcgc ggtccgccac
     3661 gcgcatgagt tcgtcggacg tggtcccgtg gtcgggatac agcaccacgc cgatgctcag
     3721 gtccaactcc tcgtcgagcg tctccccgag ttggcggacg ctccggcgaa tccggtccgc
     3781 ggtgacggcg accgcgtcgt gcgccacctc cggcagcacc accacgattt cgtccgcgcc
     3841 ccatcgaaag accagatccg tctcgcggac cgcgccctgg aggcgcttgg ccacggcctt
     3901 gagcacgtcg tcgccgacgc tgtgcccccg catgtcgttg atcgaacgaa agcggtccac
     3961 gtcgcacaac atgaccgcga gcgaccgatg agtgcgcgag gcgcgcgcca cctcttcgtc
     4021 gaagcggccg tggaagtacc gccggttgta gaggccggtc agctcgtcgt gaatcgcgga
     4081 ggcttcgagc gtggcttcgg ccttgcgtcg atcgatcgcg accgcgatca acgccgcgac
     4141 gctccgcacg aactccacgt cgtcgatcgt aaacgcgcgg ggacgacgcg cgtagaccgc
     4201 gaggaccccg atggtgcggc tgcgcgagac gagcgggacg ctcaggccgg acaccacgtg
     4261 ttgctcggtc agcaacgacg acgcggcaaa ccggcgttcg gccgcgaggt cgtccacgac
     4321 caccggaccg ttcaccgcca aggtgtatcc ctgctgcgag tcggccgccg ccggcatgac
     4381 gaacgggccc aacacgtgtt tcccccagcc gtagcccgcc acccgcacca acgtgtcgcc
     4441 ccggccgtcg agcatcagga tttccgcgag gtcggcgtcc gccccgcccg cgatgagcgg
     4501 cgtggcgcgc tccatcacct cgtccgcgct ggaggcctcc aagcaccgct cgccgacctc
     4561 cttggccatg cgctgctgct cgacccgttt gcgcaggatc gtttcggccc gtttgcgctc
     4621 ggtcgtgttc cggaagacgg cgaggccccc gatgatggac ccggtttcgc cgaagagcgg
     4681 ggcgtattgt ccttcgaaga acccgcggtc cgtgcccgcc gcggcatagg gccggtcctt
     4741 ggccaggacg gtttttccgg agagcgcgtc caacaggcag cgatcgtccc cggtctgctt
     4801 caacaacggg aagaggtcga aggccga
//
LOCUS       DBNGTT010000211         2282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1575910, whole genome shotgun sequence.
ACCESSION   DBNGTT010000211 DBNGTT010000000
VERSION     DBNGTT010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1575910"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..128
                     /locus_tag="AC16BC_04730"
     tRNA            43..128
                     /locus_tag="AC16BC_04730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Leu,seq:caa)
     gene            328..1539
                     /gene="glgC"
                     /locus_tag="AC16BC_04735"
     CDS             328..1539
                     /gene="glgC"
                     /locus_tag="AC16BC_04735"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR3240956.1"
                     /translation="MVRISQPRILAMIMAGGRGERLAPLTDFRTKPAVPFGGSYRIVD
                     FVLSNFVNSGVMAIYVLVQYRSQSLIEHLRRAWRIGGRIQQNFITVVPPQMKTGAMIY
                     EGTADAVYQNINLIHDFTPDLVAVFGADHIYRMDIRQMVDFHLSRKAQITVAALPVPL
                     ASASAFGVIEANDEDRIVGFEEKPARPKSMPRDADHAYVSMGNYLFEAHALVKVLTQD
                     AAHGGSHDFGRNIIPRLVDECRVVAYNFRTNTIPDFQSYEEQGYWRDVGTIDAYWQAH
                     MDLLGEQPRFDLRNAGWPIMSDSQDGPAASLVRTRVDASMIGQGSHVVESEIIRSVIG
                     RNVRIDPGCRIEESIILDGSIVGAGSRIRRVIADRFNVIPKQSTLGGDTISVLARGRS
                     VGGRAVNVTGR"
     gene            complement(1536..>2282)
                     /locus_tag="AC16BC_04740"
     CDS             complement(1536..>2282)
                     /locus_tag="AC16BC_04740"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR3240957.1"
                     /translation="VESVELLRRDIWTGDASVDDLPKRSITRVRRHGKSILIETSSPP
                     LWLVVRLGMTGQCLIAPRGAERTPHTHLVLTLGGEPWELHYRDPRRFGGWRATASLAD
                     IGLGPDALALRRATWQRIMAERRGMLRPLLMNQNIIAGLGNIYVNEALFAARLHPRQE
                     TSRLNEAQRDALYRAIKSTLRKGIRWGGSTIRDYRTPDGESGRFQERFRVYGRAGLRC
                     KRRCGGRIEKLSAAKDAQPGFYCPRCQRLI"
BASE COUNT          397 a          770 c          741 g          374 t
ORIGIN      
        1 cagcgacaac gcagcggatg gccccctatc gacggatcag acgccgaagt ggtggaatgg
       61 cagacacggc ggactcaaaa tccgctgagc gcaagctcgt gggggttcaa ctcccccctt
      121 cggcaccaac cgtccgtacc gtgatcaacc ccgcatcgct gattgaaccc gtagccgttt
      181 ggcgtcgatg acgccgcggt ccgcaaagcg ccgactcccc cggttgacgc ctcctcgcca
      241 cggttgacac gaagcccggt gtcggcgcaa agtaaagcta cagaccgctg gccgtcggcg
      301 gcaaccccct cgtcggagga ttccggcatg gttcgcatct cccagccccg catcctcgcc
      361 atgatcatgg cgggcggccg cggcgaacgg ttggccccgc tcacggattt tcgcaccaaa
      421 cccgcggtcc cgttcggcgg cagctaccgc atcgtggatt tcgtgctgag caacttcgtg
      481 aactccgggg tgatggcgat ctacgtgctg gtgcagtacc gctcgcaatc gttgatcgaa
      541 cacctgcgcc gcgcgtggcg gatcggcggg cgcatccagc agaacttcat cacggtggtg
      601 cccccgcaga tgaagaccgg cgccatgatt tacgaaggca cggccgacgc ggtgtaccag
      661 aacatcaatc tgatccacga tttcaccccc gatctggtcg cggtcttcgg cgcggaccac
      721 atctaccgga tggacatccg ccagatggtg gactttcacc tctcgcgcaa ggcccagatc
      781 acggtcgcgg ccttgcccgt gcccctggcc tccgcctccg cgttcggcgt gatcgaagcc
      841 aacgacgaag accgcatcgt ggggttcgaa gaaaaacccg cgcggcccaa gtccatgccg
      901 cgcgacgcgg atcacgcgta cgtctcgatg gggaattatc tgttcgaggc ccacgcgctc
      961 gtgaaggtcc tgacccagga cgcggcgcac ggcggatcgc acgacttcgg gcgcaacatc
     1021 atcccgcgcc tggtcgacga atgccgcgtg gtggcgtaca acttccggac caacacgatc
     1081 ccggatttcc agtcctatga agagcagggc tattggcggg acgtgggaac catcgacgcg
     1141 tattggcaag cccacatgga tctgctgggc gaacagcccc gctttgacct gcgcaacgcg
     1201 ggctggccca tcatgagcga cagccaggac ggcccggccg cgagcctggt gcgcacccgc
     1261 gtggacgcgt ccatgatcgg ccagggcagc cacgtcgtgg agtcggagat catccggtcg
     1321 gtgatcgggc gcaacgtgcg catcgacccc ggatgccgga tcgaggagtc catcatcctc
     1381 gacggctcga tcgtcggcgc gggctcgcgc atccgccggg tgatcgcgga ccgtttcaat
     1441 gtgatcccga aacagagcac gctcggcggc gataccatct cggtcctcgc gcgcggccgg
     1501 tcggtcggcg gacgcgccgt caacgtgacc ggacgctaaa tcagtcgctg gcagcgcggg
     1561 cagtagaatc cgggctgggc gtccttggcc gctgagagct tctcgatgcg gccgccgcag
     1621 cggcgtttgc accgcagccc cgcgcggccg tagacgcgga agcgctcctg gaaccggccg
     1681 ctttccccgt ccggggtccg gtagtcgcgg atcgtggatc cgccccaccg gatccccttg
     1741 cgcaaggtgg atttgatggc gcggtagagc gcgtcgcgtt gcgcctcgtt gaggcgtgac
     1801 gtttcttggc gcgggtgcaa tcgcgcggca aacagcgctt cattgacgta gatgttgccc
     1861 aggcccgcga tgatgttttg attcatcaac aggggccgaa gcatgccgcg acgctcggcc
     1921 atgatgcgtt gccacgtggc gcgacgcaat gccagggcgt cggggcccag accgatgtcc
     1981 gcgagcgacg cggtcgcgcg ccagcctccg aaccggcgcg ggtcgcggta gtgcagttcc
     2041 cagggctccc cgccgagggt cagcaccaga tgcgtgtgcg gcgtgcgctc cgccccgcgc
     2101 ggcgcgatca aacactgccc ggtcattccc aggcgaacca ccagccagag cggcggagac
     2161 gacgtctcga tcaagatgga tttgccgtga cggcggacac gcgtgatcga ccgcttgggc
     2221 aggtcgtcga ccgacgcgtc tccagtccag atatcgcgac gcagcagttc gacggactcg
     2281 ac
//
LOCUS       DBNGTT010000212         3442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_938344, whole genome shotgun sequence.
ACCESSION   DBNGTT010000212 DBNGTT010000000
VERSION     DBNGTT010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_938344"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="AC16BC_04745"
     CDS             complement(<1..441)
                     /locus_tag="AC16BC_04745"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF016382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003727 - single-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HIR3240958.1"
                     /translation="MQREMAARVVRSTRNRGIRYVAGADAAFVEGRGLALASVVVLGF
                     PRLDVVEAVEGTAPLDFPYIPGLLSFREVPALLAAFAKLRTAPDVVLCDAHGLAHPRR
                     FGLASHLGVALDVPTIGCAKSRLYGVATEPAPAAGSTTPLVDPRD"
     gene            586..1371
                     /locus_tag="AC16BC_04750"
     CDS             586..1371
                     /locus_tag="AC16BC_04750"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HIR3240959.1"
                     /translation="MVVRVECYAGHRGEETPRAFFLDARRVEVAEVLDRWHGIDHRYF
                     KVRGADGGLYILRHDTSADRWEITLFQQAGTAAETAWPEPTRYGEAAPPIRALPLALA
                     IFRGTAPADVAALIEELAGLGLHVTRRTQAELPPEAFDLARGQYRGSGVLTRIQPEPG
                     ERVLAVTEADLYDDGLNFIFGLADPGRRVALISFHRLRLLADPWTFRARAAKAAVHEL
                     GHTLGLSHCSDPACVMFFSNTLADTDRKGARFCPRCHRGLSWH"
     gene            2227..3204
                     /locus_tag="AC16BC_04755"
     CDS             2227..3204
                     /locus_tag="AC16BC_04755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3240960.1"
                     /translation="MPVRIATFNLENLDDGVAVQPALAERIHIMRPQLERVAADILCL
                     QEIHAQGPAGSRMLAALDQLLSGTPYAGFHRQVTLTTTEQLYDQRNLVTLSRFPIVRT
                     HMIRDSAGPRPRYQMATANPPDSTANPLEWERPMLYTQIDMGSGRTLHLINVHLKSKL
                     ASMIPGQKIDNFTWRTVSAWAEGSFISSMKRVGQALQARLLIDDIFDTHGPGSLIAIA
                     GDFNAEAEEVSFKAICGLVEETGNPAHAARVMVPCENNIPDSARYSLFHLGKGQMLDH
                     IVVSRPLLKYFRGAEIHNEALPDESGAFRQDTKFPESDHAPVVAEFDLD"
BASE COUNT          646 a         1135 c         1005 g          656 t
ORIGIN      
        1 gtcacgcggg tcgaccagcg gcgtcgtgct ccccgccgct ggggccggct ccgtcgccac
       61 tccgtacaag cggctcttcg cgcaaccgat cgtgggcacg tcgagagcaa cgcccagatg
      121 gctcgccaac ccaaagcgcc tcgggtgggc aaggccgtgg gcgtcgcaca gcaccacgtc
      181 aggagcggtg cgcagcttgg cgaacgcggc cagtaaggcg ggaacttccc gaaacgacag
      241 cagccctgga atgtacggga agtccagcgg cgcggttccc tccaccgcct cgaccacgtc
      301 caatcgggga aaccccaaca ccacgacgct ggccagggcg agcccccggc cctccacgaa
      361 cgccgcatcg gccccggcca cgtagcggat cccgcggttt cgcgtcgatc gcaccacgcg
      421 agcagccatc tcgcgctgca tggcgatcgc ctcccggggg ttagggacga ccacccggat
      481 cctgccattg tgttctcaac atccgtctcg ctccttctca acctgactgg cgccttcgcg
      541 agcgcccctg gaaccgcgcc ggcatcgtgc agtatgctgc ggtccatggt ggttcgggtc
      601 gaatgttacg ccggccatcg cggcgaagaa acgccgcgtg cgttcttcct ggacgcacgg
      661 cgggtcgaag tggccgaggt gctggaccgc tggcacggga tcgatcaccg atacttcaag
      721 gtccgcggcg ccgacggcgg cctgtacatc ttacgacacg ataccagcgc cgaccgctgg
      781 gagatcacgc tgtttcagca ggccggcacc gcggccgaga ccgcttggcc cgagcccacg
      841 cgctatggag aggcggcgcc gccgatccgt gccctgccgc tcgcgctcgc catctttcgc
      901 gggacggccc cagccgacgt cgcggccctc atcgaggagc tggcgggcct cggcctccac
      961 gtcacgcggc ggacccaggc cgagctgcca ccggaggcgt tcgacctggc gcgcggccag
     1021 taccgcggct cgggcgtgct gacccggatc cagcctgaac cgggggagcg cgtcctggca
     1081 gtgaccgagg ccgatctgta cgacgacggc ctcaatttca tcttcggctt ggccgaccca
     1141 gggagacgcg tcgccctgat ctcgtttcac cgcctgcggc tgctggccga tccgtggacg
     1201 tttcgcgccc gcgccgcgaa ggcggcggtc cacgagttgg gccatacgct cggcctgagc
     1261 cactgctccg accccgcctg cgtgatgttt ttcagcaata cgctcgccga cacggaccgc
     1321 aagggcgccc gcttttgtcc gcgttgccac cgcgggctct cctggcactg agaccagatc
     1381 tccacccatt gctcggacca actcaatcta ctgctgctgg gttagaaaag tagcgtcgag
     1441 gggctggacc tgagcgcgat caccgcggtc tatgagcgtg tcactggcga attgttcgtc
     1501 accgctaaaa atgccgtcga gtccagatgt taagttggcc ccaaccccgg gtgctcctag
     1561 ccgtcgtacc ggcacggcct cccgggttca cctcaccaat ggacttcacc ccaaggacat
     1621 ggtgccaagc gtggtgccaa actaccctgg gtctgccaga ccaagacgac cgatcatccg
     1681 ggcggacctc ttgcttttgt tggcgattcg tcaggatcgg tggggttggt ctgatctgaa
     1741 ttttgttcgt aatcagccgg tcgtcggttc aatcccgacc gccggctcca atcaaatcaa
     1801 agacttcgag gggagagaag gtgacctgct gataaggggt catcagtcgg ttgcggatag
     1861 ttgcggatgt tcgctcgatt ttgcctgttc tccgaattag gccatcgaaa ttaggccatc
     1921 gaattaactg catcacaccg ccctatccta agaagcctga tctataacta ctctcgccgt
     1981 cggtattgca caacgactgc tgcccgctcc actccaagag ttcgtcttcc gctttttcaa
     2041 cccaatcagc aggtcacaag cggctgacgc tgccatcgtg tatctcatta gattacccgt
     2101 gttatctgta tcgataacag caggtcaagc aaggtctccc cgctcacctc atgcaccgtc
     2161 ccttcgatgc gcccatcacg gcatacttcc tgcctcgata ccagatagga caagggagga
     2221 cgttccatgc cagtgcgtat agccaccttc aatctcgaaa acctcgacga cggcgtcgcc
     2281 gtgcaacccg ctctcgcgga gcgcatacac atcatgcgtc cccaactcga gcgcgttgcg
     2341 gcagacatcc tctgtttaca agagattcac gcccagggtc cggctggttc caggatgttg
     2401 gccgcccttg atcagctact gagcggcaca ccttacgcgg ggtttcacag gcaggtgacg
     2461 ctcaccacga ccgagcaact ttatgatcag cgtaacctcg tgaccctttc gcgctttccg
     2521 atcgtcagga cgcacatgat ccgcgacagc gcggggcctc ggccacgcta tcagatggcg
     2581 actgccaatc cacccgactc gacggcgaat ccgctggagt gggagcgacc catgctgtac
     2641 acgcaaattg acatgggatc tggtcgcacg ctgcatctaa tcaacgtgca tctcaaatcc
     2701 aagctcgcct ccatgattcc gggccagaag atcgacaatt tcacgtggcg aaccgtgtca
     2761 gcgtgggccg agggcagctt tatctcatcg atgaaacgcg tcggccaggc gcttcaggcg
     2821 agattgctga tcgacgacat ctttgatacg cacggccctg gctcgctcat tgcgatcgcc
     2881 ggggacttca acgccgaggc cgaggaagtg tcgttcaagg cgatctgtgg cctcgtcgaa
     2941 gagaccggca atcccgccca cgctgctcgc gtgatggttc cctgcgaaaa caatatccct
     3001 gattcggctc ggtattccct cttccatctc ggaaaaggcc agatgctgga tcacatcgtc
     3061 gtgtccaggc cactcctgaa gtactttcgt ggcgcggaga ttcacaacga agccttgccc
     3121 gacgaatcgg gagccttcag acaggacacc aaattcccgg agtcagatca cgcgcccgtg
     3181 gtggcggagt ttgatctcga ctgatccgcg ccgagacgga gaccgcgcga gcaagaccac
     3241 gggacagtat tagattagcc gtacgccgcc ttcagtggcc cacacaaggg atcagacgga
     3301 gaatggggag attcgcatcg tcatacttca tggcagtcgt ctgttcggga gaggctcgcc
     3361 ctcgttttgg cccaacaggc gggattctct gtggtcgggg tttcggctgg attcgatcag
     3421 gctgaccgag aactcgcttc tg
//
LOCUS       DBNGTT010000213         3128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1960758, whole genome shotgun sequence.
ACCESSION   DBNGTT010000213 DBNGTT010000000
VERSION     DBNGTT010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1960758"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="AC16BC_04760"
     CDS             <1..973
                     /locus_tag="AC16BC_04760"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3240961.1"
                     /translation="QLARVYEAERDFDAALKLYEKVLSQDFAFRDARDRAAQVKTAKA
                     APPPSKTTSSSTPQTMPRYKILKQIGQGGMGLVYRAEDTVLHRVVAYKVLPTSVKEQP
                     AVLENFLQEARIAASINHPNIVTIYDTGHDPAGEVFISMEFVDGLSLKELLDKMGALP
                     LPQFLSIAKQICAGLGYAHGRRIIHRDIKPSNIMLSRDRMVKIMDFGLAKIVNDAIAD
                     RTSVKGTPLYMAPEQILGKEVDHQSDLYSLGCTLYRMATGRLPFTKGDLYYQHLHTRP
                     QPPKELNPKLPSAINAIIMRCLEKEKAKRYQQVGDVLADIEQVKLAA"
     gene            1055..1759
                     /gene="queC"
                     /locus_tag="AC16BC_04765"
     CDS             1055..1759
                     /gene="queC"
                     /locus_tag="AC16BC_04765"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR3240962.1"
                     /translation="MPTPRRAVLLLSGGLDSAVSLGILRAEGWVVHALTVDYGQRHAV
                     ELAQAGALASHYGVDHRVVAVDLRAIGGSALTDTIDVPTDRVPGSEGIPVTYVPARNT
                     ILLALALAWAEVLDAGAIAIGANAVDYSGYPDCRPEFLAAFQGLTRLATRAGVESGGG
                     IDVLAPLSGFSKTEIIRRGAALRVPFALTHSCYAPVGGLACGRCESCRIRRDGFASAG
                     VLDPTHYAPSVTGVRS"
     gene            1837..2424
                     /gene="folE"
                     /locus_tag="AC16BC_04770"
     CDS             1837..2424
                     /gene="folE"
                     /locus_tag="AC16BC_04770"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR3240963.1"
                     /translation="MANGGDSNTAPQIEALITTLLSLLGEDPQRDGLVNTPGRVERAL
                     RQFTSGYHQDVADILNGALFPIEYNEMVIVRDIDFYSLCEHHLLPFFGKCHVAYLPNK
                     KIIGLSKLPRLVDVFSRRLQVQERLTQQIAETIQEHIEPLGVAVVMEGQHLCMMMRGV
                     EKQNTLAVTSAMLGVFKTDYKARGEFLDLIRRRTS"
     gene            2421..2795
                     /gene="folB"
                     /locus_tag="AC16BC_04775"
     CDS             2421..2795
                     /gene="folB"
                     /locus_tag="AC16BC_04775"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR3240964.1"
                     /translation="MTDRLIISGLALFTHCGIMPEERAVGQRVVADVDMSIEMDHAVG
                     PGGLETTVDYADVCRQITEIADKARHVLLESLAIEVADATLARPRVHEVRVRITKPTP
                     PITPAVAAFAVEVVRRRSHARS"
     gene            2805..>3128
                     /locus_tag="AC16BC_04780"
     CDS             2805..>3128
                     /locus_tag="AC16BC_04780"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIR3240965.1"
                     /translation="MAFWGLEHKIHLPKPEEALPGRSEAMPVPTRHVVTGQPLQPPFP
                     NGFERAMFGMGCFWGAEKRFWQTKGVYATAVGYAGGITRNPTYDEVCTGLTGHNEVVL
                     VVYDPA"
BASE COUNT          586 a          920 c         1018 g          604 t
ORIGIN      
        1 ccagctcgcg cgagtctacg aagccgagcg cgattttgac gccgcgctca agctctatga
       61 gaaagtgttg tcccaggatt ttgccttccg ggacgcgcgc gaccgggccg cgcaggtcaa
      121 gaccgccaag gcagcaccgc caccgtcgaa gacgacgtcg tcttcgacgc cccaaaccat
      181 gccccgctac aagatcctca agcagatcgg ccagggcggg atggggcttg tgtatcgcgc
      241 cgaggacacc gtcctccatc gagtcgtcgc gtataaagtt ttgccgacaa gcgtgaaaga
      301 gcagcccgcc gtgctggaga actttcttca ggaagcgcgg atcgcggcgt cgatcaacca
      361 tcccaatatc gtgacgatct acgacacggg gcacgatccc gccggagaag tgttcatctc
      421 gatggagttc gtcgacgggc tcagtttgaa ggaattgctg gataagatgg gcgcgcttcc
      481 gcttccgcaa tttctgtcca ttgccaaaca gatctgtgcg ggcctgggct acgcccatgg
      541 acgacgcatc atccatcgcg acatcaagcc gtcgaacatc atgctgagcc gcgatcgcat
      601 ggtcaaaatc atggacttcg gcttggcgaa gatcgtcaac gacgcgatcg ccgaccgcac
      661 ctcggtcaaa ggaacgccgc tgtacatggc gcccgagcaa attctgggca aggaagttga
      721 tcaccagtcc gatctgtatt ccttggggtg tacgctctac cgcatggcga cgggtcggtt
      781 gccgttcacc aaaggagact tgtactacca acaccttcat acccgcccgc agcctcccaa
      841 ggagctgaat ccgaaactac cttccgccat caacgcgatc atcatgcgat gtctggagaa
      901 ggagaaggct aagcggtatc aacaggtggg tgacgtgctg gccgatatcg agcaggtcaa
      961 gctcgccgcc tagttcctcc ttcctctccg cccctcttcc ttgctgcccg tgaagcgggg
     1021 gtgatatact ccactccctt tcgccgaggg aggaatgcca acaccgcgcc gtgccgtctt
     1081 gttgttgagc gggggactcg actccgcggt ttcgctggga atcctgcgag ccgaggggtg
     1141 ggtcgtccat gcgctgaccg tggactatgg gcagcgccat gcggtggaac tggcccaagc
     1201 tggcgccctg gcgtcgcatt acggagtcga ccaccgcgtg gtggcggttg atcttcgcgc
     1261 gatcggcggc tccgcgctga ccgacacgat cgacgtgccg acggatcgtg tgccgggaag
     1321 cgagggcatt cccgtcacgt atgtcccggc gaggaatacg atcttgttgg cgctggcctt
     1381 ggcgtgggcc gaggtgttgg acgcgggcgc gatcgcgatc ggagccaacg cggtggacta
     1441 ctcgggatac ccggactgcc ggcccgagtt cctggccgca ttccagggac tcacccggtt
     1501 ggcgacgcgt gccggagtcg agtcgggcgg cgggatcgac gttctcgcac cgttgagcgg
     1561 attcagcaaa actgagatta tccgccgtgg cgcggcgttg cgcgtgccgt ttgcgctgac
     1621 gcacagttgt tacgctccgg tcggaggcct agcgtgtgga cgctgcgaga gttgccggat
     1681 ccgacgtgat gggttcgcgt ccgccggtgt attggatccg acacattacg ctccgtccgt
     1741 caccggagtc cggtcgtgat cgagcgagag cgaacccgcg gggtgagcgg cttgtcggat
     1801 ggacgggatt ccgatttcga aggggtaacg catcccatgg cgaacggtgg tgactcgaat
     1861 acggcgccgc agatcgaagc cttgatcacg acgctgctgt ccttgctcgg ggaagaccct
     1921 cagcgggacg gtctggtgaa cacgccgggt cgtgtcgagc gggcgctccg ccaatttacg
     1981 tcgggctacc accaggacgt ggcggacata ctcaatggcg cgctttttcc catcgaatat
     2041 aacgagatgg tcatcgtgcg ggatatcgat ttttactcgc tgtgcgaaca tcatcttttg
     2101 ccgtttttcg ggaagtgtca cgtggcctat cttcccaaca agaagatcat cgggttgtca
     2161 aagcttcccc gattggtcga cgtgttcagc cgccgcctgc aagttcagga gcggctgacg
     2221 caacagatcg cggagacgat tcaggagcac atcgagccgc tcggggtggc agtggtcatg
     2281 gagggacaac acctctgtat gatgatgcgc ggggtcgaga aacagaatac gcttgcggtc
     2341 accagcgcga tgctgggcgt cttcaagacc gattacaagg cgcggggcga attcctcgat
     2401 ttgatccgcc gccggacgtc gtgaccgatc gactaatcat ctctggtctc gcgttgttca
     2461 cgcactgcgg gatcatgccg gaggagcgcg cggtgggtca gcgggtcgtc gccgacgtgg
     2521 acatgtcgat tgaaatggat cacgccgtcg ggccgggcgg actcgagacc accgtggatt
     2581 acgcggacgt ctgccgccag atcaccgaaa tcgcggacaa ggcccggcac gttctgttgg
     2641 agtccctggc gatcgaggtg gcggacgcca cgctggctag gcccagggtc cacgaggtcc
     2701 gagtccggat caccaagccg accccgccga tcacgcctgc ggtcgcggcg ttcgcggttg
     2761 aagtcgtgag gcgcagatcg catgcgcgga gttgaagcct gaccatggcg ttctggggac
     2821 ttgaacacaa gattcacctg ccgaagccgg aggaggcgtt gccgggtcga tcggaagcga
     2881 tgccggtccc gacgcggcac gtggtcactg gacaaccgct ccagccgccg tttccaaatg
     2941 ggttcgagcg cgccatgttc ggcatgggct gtttttgggg cgccgagaag cggttctggc
     3001 agaccaaagg ggtctatgcg accgcggtgg gttatgccgg tggcatcacc aggaacccga
     3061 cgtatgacga agtctgcacg gggttgaccg ggcacaacga ggtggtgctc gtggtgtacg
     3121 atcccgcg
//
LOCUS       DBNGTT010000214         3084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_427768, whole genome shotgun sequence.
ACCESSION   DBNGTT010000214 DBNGTT010000000
VERSION     DBNGTT010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_427768"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..965
                     /locus_tag="AC16BC_04785"
     CDS             162..965
                     /locus_tag="AC16BC_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIR3240966.1"
                     /translation="MIVEKRAETRGIYTFRLRIRDEAQRAAFRFRPGQFNMLYVFGVG
                     EIPISIASDPDDSDALDHTVRIVGNVTGVLERLSAGDAVGLRGPYGSSWPLGEAQGRD
                     VILITGGLGCAPVVSVISYVVRRRDRYGALKILHGVKAPKDLLYRERFTAWAARPNTE
                     VHLTTDQPDRRWRHHTGVVTGLLDNLAIAPFNSVVMMCGPEGMMRAAAAYLFNAGVPA
                     ERVYVSIERNMKCAVGLCGHCQMGSEFVCKTGPVFRYDRIRQWFEHPGV"
     gene            1194..1976
                     /locus_tag="AC16BC_04790"
     CDS             1194..1976
                     /locus_tag="AC16BC_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR3240967.1"
                     /translation="MPKRGSRRPKLAVWKFASCDGCQLTLLNCEDELLAIADLVRIAE
                     FREASRAAIRGPYDLSLVEGSITTAADAERVKQIRAASRRLVTIGACATAGGIQALRN
                     FKDVNEFVSVVYATPAYISTLAASTAIADHVPVDFELRGCPIDPGQLREVIRAFLQGR
                     RPQTPASSVCLECKRAGRVCVMVAQGTPCLGPVTHAGCGALCPTYGRGCYGCFGPMET
                     PNTASLAAELSRRGVPGRELARAFATFNADAPAFRKESAKYG"
     gene            1969..>3084
                     /locus_tag="AC16BC_04795"
     CDS             1969..>3084
                     /locus_tag="AC16BC_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR3240968.1"
                     /translation="MARRTIRVGSLARVEGEGGLEVTVKDGAVTDVKLDIFEPPRFFE
                     ALLRERRFDEAPDITSRICGICPVAYQMSAIHAMEDACGVRVEGQLRALRRLMYCGEW
                     IESHTLHVYMLHAPDFLGYASGIEMAKDHPAIVERGLQLKKVGNEIIALLGGRSVHPI
                     NAKVGGFYRVPTSEELAALAERLRWARDAALDTVHWTSTLPIPDYERDYVFVAVSHPD
                     EYPFNEGRIVSNHGLDISAREYDDHFAESQVPHSTALHSALSGRGAYAVGPLARYSLN
                     FDRLSPIAQDAARRAGLGLACRNPFQSIVVRSVEILYACDEALRIIDQYEPPEAPAVE
                     ARPRAGTGYGCTEAPRGLLYHRYRIDEQGTILDAKIVP"
BASE COUNT          483 a          949 c         1123 g          529 t
ORIGIN      
        1 tgcggccgct gcatcgcgtg gtgcccggtg gggatcgata tcaccgaaga agtccgcgcg
       61 atccggagcg gcacgaggtg accgactcat tcgccgcggc gtctgccgta ggggttcgac
      121 atgtcgaacc cctgccaaat cgggctatcc cgatccccgc cgtgattgtg gagaaacgcg
      181 cggagacccg cggcatctac acgtttcggc tccggattcg ggacgaggcc caacgcgcgg
      241 cgtttcggtt tcgtccgggc cagttcaaca tgctgtacgt gttcggggtc ggcgaaattc
      301 cgatctccat cgcgtccgat ccggatgatt cggacgcgct cgatcacacg gtgcggatcg
      361 tgggcaacgt gaccggcgtg ctggagcggt tgagcgcggg cgacgcggtg gggctgcggg
      421 ggccgtacgg cagttcttgg cccttgggcg aggcgcaagg ccgcgacgtg atcctgatca
      481 cgggcggact cggctgcgcg ccggtggtgt cggtgatcag ctacgtggtg cgacgacggg
      541 accggtacgg cgcgctcaag atcctgcacg gggtcaaggc gcccaaggat ctgctctacc
      601 gcgagcggtt cacggcgtgg gccgcgcgcc ccaataccga ggtgcacctg accacggatc
      661 aaccggaccg gcggtggcgc caccacaccg gggtggtgac ggggttgttg gacaacctgg
      721 cgattgcgcc gttcaacagc gtggtgatga tgtgcgggcc cgagggcatg atgcgcgcgg
      781 ccgccgcgta cctgttcaac gccggcgtgc cggccgagcg cgtgtacgtg tcgatcgagc
      841 gcaatatgaa gtgcgcggtg gggttgtgcg ggcattgtca gatggggtcg gagtttgtct
      901 gtaagacggg gccggtgttt cggtatgacc gcatcaggca gtggttcgaa catccgggtg
      961 tgtagagcct gtccatgacg ggtcatctgc ggcgttgtcg ctgcgcgtcc gctcctcacg
     1021 tacgacccag tacgctgcgg tgcgggcgct cgctccgcct tgcatctgac accgtcctga
     1081 acaggctccg attcctgcgg aatcgggaaa agtgacagga aaacatgccg cagtctgcaa
     1141 atagagttgg ggcggccggt cggtcgtcgg tggcgagaaa tgcaaaggcg aagatgccga
     1201 agcggggctc tcgtcggccc aagctggcgg tttggaagtt tgcgtcttgc gacgggtgtc
     1261 agctgacgct gctcaattgc gaggatgagt tgctcgcgat cgccgacttg gtgaggatcg
     1321 cggagtttcg agaggcgtcg cgcgcggcga tcagagggcc gtatgatctg tcgctggtcg
     1381 aggggtcgat taccacggcg gcggacgcgg agcgcgtcaa gcagatccgc gccgcgtcca
     1441 ggcgactggt caccatcggc gcgtgcgcga ccgcgggcgg gatccaggcg ttgcgcaact
     1501 tcaaggacgt gaacgagttc gtgtccgtgg tgtacgccac gcccgcctat atcagcacgc
     1561 tggccgcgtc caccgcgatc gcggatcacg tgccggtgga ttttgagttg cgcgggtgcc
     1621 ccatcgaccc cgggcagctt cgcgaagtga tccgcgcgtt cctccagggc cggcggccgc
     1681 agactccggc gtcgagcgtg tgcctggagt gcaaacgggc cggacgcgtg tgcgtgatgg
     1741 tggcgcaggg cacgccgtgc ctgggcccgg tcacgcacgc gggctgcggt gcgttgtgtc
     1801 cgacctacgg gcgcggatgt tacgggtgct tcggcccgat ggaaacgccg aacaccgcgt
     1861 cgctcgccgc ggagttgtcc cggcgcggcg tgccggggcg ggaactcgcg cgcgcgttcg
     1921 ccacgttcaa cgcggacgcg ccggcctttc gcaaagagtc ggcgaaatat ggctaggcgc
     1981 acgatcaggg tcgggtcgct cgcgcgcgtc gagggcgagg gcggactcga agtcacggtc
     2041 aaggacggcg cggtcaccga cgtgaagctc gacatcttcg aaccgcctcg gtttttcgag
     2101 gcgctgctgc gcgagcggcg gttcgacgaa gcgccggaca tcacgtcgcg catctgcggc
     2161 atttgcccgg tggcgtacca gatgagcgcg atccacgcca tggaggacgc gtgcggggtg
     2221 cgggtcgagg gccagcttcg cgcgttgcgg cgtttgatgt actgcgggga gtggatcgag
     2281 agccacacgc tgcacgtgta catgctgcac gcgccggact ttctcgggta cgcgagcggg
     2341 atcgagatgg ccaaggacca tcccgcgatc gtagagcgcg ggctgcaact caaaaaagtc
     2401 gggaacgaga tcatcgcgtt gctgggcggg cgctccgtgc acccgatcaa cgccaaggtc
     2461 ggcgggtttt accgggtgcc cacgtccgag gaactcgcgg cgctcgccga gcgactgcgt
     2521 tgggcgcgcg acgccgcgct ggacacggtg cattggacgt ccacgctgcc gattccggat
     2581 tacgaacggg attacgtctt cgtggccgtg tcgcacccgg acgagtatcc gttcaacgag
     2641 ggacggatcg tgtccaacca cgggctcgat atttccgcgc gcgagtacga cgaccatttt
     2701 gccgagtccc aggttccgca ctcaaccgcg ctgcactccg cgctctcggg ccgcggcgcc
     2761 tacgccgtgg ggccgcttgc ccgctacagc ctcaacttcg accgcttgtc gccgatcgcg
     2821 caggacgcgg cgcggcgcgc cggcttgggc ctggcgtgcc gcaatccgtt tcagagcatc
     2881 gtggtgcgca gcgtggaaat cctctacgcg tgcgacgagg ccctgcggat catcgaccaa
     2941 tacgagccgc ccgaggcgcc ggcggtcgag gcgcggccac gggccggcac cgggtacggc
     3001 tgcaccgaag cgccgcgcgg tctgctctac caccgctacc ggatcgacga gcagggcacg
     3061 atcctggacg cgaagatcgt gccg
//
LOCUS       DBNGTT010000215         2497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2089584, whole genome shotgun sequence.
ACCESSION   DBNGTT010000215 DBNGTT010000000
VERSION     DBNGTT010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2089584"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="AC16BC_04800"
     CDS             <1..540
                     /locus_tag="AC16BC_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HIR3240969.1"
                     /translation="DCAHPRAGDCVIAQVAHFQRVLADSRRPRAGRAEALRFLTHLVV
                     DLHQPLHSADNGDRGGNDVLVTFFGEVLNPFSGKPWNLHAVWDGGLIERRGLSVSEYA
                     RRLTRRIGRGRAAEMERGTVADWALESHRAAVATSYRLPPDRNLGDEYADQARPVIDD
                     MLAKAGVRLAKVLNDALTP"
     gene            727..1509
                     /locus_tag="AC16BC_04805"
     CDS             727..1509
                     /locus_tag="AC16BC_04805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3240970.1"
                     /translation="MLIDTHTHVHGVEFDSDREPALTRARSAGVAACLAIGTDVADSR
                     RAVDLAAQQPDVFASVGVHPHETAGLSDLGLEELSRLAAGDRVVAFGEVGLDYYYEHS
                     PKDIQRQRFADQVDIAARLGLPLVIHTRDAWDDTFRILDERRHGGGVFHCFTGTREHA
                     EEAVRRGFFVSFSGIVTFHKAQALQAVAREVPLDRMLIETDCPFLAPVPHRGARNEPA
                     FVALVARKIAELRGLSIEEVSRATTANARSCFPRLSKVLPPS"
     gene            1558..>2497
                     /locus_tag="AC16BC_04810"
     CDS             1558..>2497
                     /locus_tag="AC16BC_04810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3240971.1"
                     /translation="MELPAQFGKYRLLHRVGHGGMAELYLAMQTGLKGFEKVLAIKKI
                     LPHLTQDSEFVAMFVNEGKLAALLTHQHIVQIFDLDHVNGVYYIAMEYVMGKDLRTVA
                     VRARERGGQFPIDLALLICSQVASGLDYAHRRKDLDGRELNIVHRDVSPQNILVSYEG
                     EVKLVDFGIAKVAGIGQETKTGILKGKLAYMSPEQAMGRPIDRRSDVFALGIVLYEVL
                     TGRRLFKGDSDLSTLEAVRTANVEPPTKFDANIPVELEQVVMTALAREAGERYQTSAD
                     FQAALEGVLSARGGASTLQLAQFMTSLFGEDQRKDHE"
BASE COUNT          410 a          761 c          847 g          479 t
ORIGIN      
        1 gattgcgccc atcctcgcgc cggagattgc gtcatcgctc aggtggcgca ttttcagcgc
       61 gtgctggccg attcccgtcg gccccgcgcc gggcgggctg aagcgctcag gttcctcacg
      121 catctcgtcg tggatctgca ccagccgttg cacagcgcgg acaatggaga ccgtggaggc
      181 aacgacgtgc tggtgacctt cttcggcgag gtgctgaacc cgttctccgg gaagccctgg
      241 aatctgcatg cggtgtggga cgggggattg atcgagcggc gaggtctcag cgtctcggag
      301 tatgccaggc ggctgacgcg ccgtattggg cgtggccggg cggctgagat ggaacgcggc
      361 acggtggcgg actgggcgct cgagtcccac cgagccgcgg ttgcaaccag ctaccggttg
      421 ccgcccgacc ggaatctcgg cgatgagtac gccgatcagg cgcgtcccgt gatcgacgac
      481 atgctggcca aggccggcgt ccgtctcgcc aaagtgttga acgacgcgct cactccatga
      541 gatctcgccg ccggaccgtt ccttgaacgt gttccgtcgc aagtggtatc gtcatgccct
      601 tgtgacgacc ggtagcccga tgacaagtgg aatccttccg cactcttgcg gcggtgaccg
      661 tgtgtcgacc gttggtcctg agacagtgcc gcgcccacgc cggccgtcgt gccgtccgtt
      721 gcttccatgc tgattgacac ccacacccat gttcacggcg ttgaattcga ttccgaccgt
      781 gagcccgcgc tgactcgggc ccgctccgca ggcgtggcgg cgtgcttggc gatcggaacc
      841 gacgtggcgg acagccgtcg cgccgtggac ctggcggccc aacaaccgga tgtgttcgcg
      901 tccgtcggtg tccatccgca cgaaaccgcg gggttgtccg atctggggtt ggaggaattg
      961 tcgcgtctgg ccgcgggcga ccgggtggtg gcgttcggtg aggtcggtct ggactattac
     1021 tacgagcact ctccaaagga tatccagcgc caacggtttg ccgatcaggt cgatatcgcg
     1081 gcaaggctcg gtcttccgtt ggtgatccat acgcgcgacg cctgggacga cacgtttcgg
     1141 atcctggacg agcgccggca tggcggggga gtcttccact gctttacggg aacgcgcgag
     1201 cacgcggagg aagcggtccg tcgcgggttt tttgtgtcgt tttccgggat cgtgacgttt
     1261 cacaaggccc aggccctgca ggccgtggct cgcgaggtgc cgttggatcg gatgctgatc
     1321 gagacggatt gtccgttcct ggcgccggtt ccgcatcggg gagcgcgcaa cgagccggcg
     1381 tttgtcgcgc tcgtggcccg caagatcgcg gagttgcggg ggctgtcgat cgaggaggtg
     1441 tcgcgcgcaa ccactgcgaa tgcccgctcc tgtttccccc gtctttccaa ggtcctacca
     1501 ccttcctaaa ctttgacacc ccttccgtcg gctgctacct tctcggtggc gtatccgatg
     1561 gaacttccag cgcaattcgg caaatatcgg ctcctgcatc gcgtgggaca cggcgggatg
     1621 gcggagctct atctggccat gcagaccggt ctcaaggggt tcgagaaggt cttggccatc
     1681 aagaagatcc ttccgcacct cacgcaggat tccgaattcg tcgccatgtt cgtcaacgag
     1741 ggcaagctcg cggccctgct gacgcaccaa cacatcgtgc agatcttcga cctggatcac
     1801 gtcaacggcg tgtattacat cgcgatggaa tacgtgatgg gcaaggacct gcgcacggtc
     1861 gcggtccggg cgcgcgaacg cggcgggcag ttcccgatcg atctggcgct gttgatctgc
     1921 agccaggtcg ccagcgggtt ggactacgcg catcgccgga aggatctcga cggtcgggag
     1981 ctcaacatcg tccatcgcga cgtgagtccg cagaatatcc tggtgtccta cgagggcgaa
     2041 gtcaaactgg tggacttcgg catcgccaag gtcgcgggga tcgggcagga gacgaagacc
     2101 gggattctca agggcaagtt ggcgtacatg tcgcctgagc aggcgatggg gcgccccatt
     2161 gaccggcggt ccgacgtgtt cgcgctcggc atcgtgctgt acgaggtgtt gacggggcgc
     2221 cgtctgttca agggcgacag cgatctgagc acgctggagg cggtccgcac tgcgaacgtg
     2281 gagcccccca ccaagttcga cgcgaacatt ccggtggaac tggagcaggt ggtgatgacc
     2341 gcgttggccc gcgaagccgg cgagcggtac cagacatcgg ctgactttca ggccgcgctg
     2401 gagggcgtgc tgtcggcccg gggcggcgcg agcacgctgc aactcgcgca attcatgacc
     2461 tcgctgttcg gcgaggatca acgcaaggat cacgagc
//
LOCUS       DBNGTT010000216         8106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2344147, whole genome shotgun sequence.
ACCESSION   DBNGTT010000216 DBNGTT010000000
VERSION     DBNGTT010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2344147"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /gene="purQ"
                     /locus_tag="AC16BC_04815"
     CDS             <1..463
                     /gene="purQ"
                     /locus_tag="AC16BC_04815"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HIR3240972.1"
                     /translation="GRGGLTLGICNGFQILVESGLLPGALLRNASLKFICRTVGVRVE
                     RTDTPFTSRYVVGQVLRLPIAHADGAYWADADTLARLRDQGQIVFRYAPLDDGVANPN
                     GSIDAIAGICNETRNVLGMMPHPERASESALGGYDGRALFESALGALCPTA"
     gene            480..2738
                     /gene="purL"
                     /locus_tag="AC16BC_04820"
     CDS             480..2738
                     /gene="purL"
                     /locus_tag="AC16BC_04820"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR3240973.1"
                     /translation="MSLEHVLSEPLDHSLLTGHGVTADEYRRFRERLGRAPTLVELGI
                     LSVMWSEHCSYKSSRVHLRRFPTTGDEVVQGPGENAGAVDIGDGLAAVFKIESHNHPS
                     FIEPFQGAATGVGGILRDVFTMGARPIALLDSLRFGPLDEPRTKYLFERVVAGIAGYG
                     NCVGVPTVGGEVAIQDLYRSNPLVNVFCLGLARKDRLFLGRAAGVGNPVIYFGAKTGR
                     DGIHGATMASEAFGDGASEKRPMVQVGDPFMEKLLIEACLELMAGDLIVGIQDMGAAG
                     LTSSSCEMAGRAGTGVEIDVALVPTRETGMTPYEIMLSESQERMLLVAKPGCEEKVRA
                     VCEKWDLDVAVIGRVTDTSRLVVRRGQAIAADLPIDLLTDDAPKYERPIAPPPYLDSL
                     QGLSLEALPEPKDYAEALLTVLGSPTVASKAWVYRQYDHMVGTNSIVLPGSDAAVLRV
                     KGTDTALAVSVDGNSRYCMLNPYVGGMIAIAEACRNVVCSGARPLGASDCLNFGNPER
                     PEVMWQFALAVDGMSEACRAFGVPVVSGNVSFYNETRGIGIYPTPVIGVVGKAPVEKV
                     TTQAFKASGRAVILLGETKEELGGSQYLATIHHREQGLPPELVLEAELALYRCVLEVT
                     GEGLVESAHDCSDGGLAVALAECCVSAESSVGARVALGNPGMIRRDAILFGESQSRVV
                     LSVKPDHAERVLAVAAQWGVPAARIGETGGDRLSIAVDGDALVDVELSAVRSAWSDGL
                     SNQLGASACASR"
     gene            2738..4141
                     /gene="purF"
                     /locus_tag="AC16BC_04825"
     CDS             2738..4141
                     /gene="purF"
                     /locus_tag="AC16BC_04825"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3240974.1"
                     /translation="MPFDKMREECGIFGVYGHPEAANLTYLGLHALQHRGQEGSGIVS
                     SDGRDYYVEKGPGLVLEVFDKSRMARLPGRMAIGHNRYSTAGGSHEKNLQPFLINFAR
                     GHLALAHNGNLVNAQGLRSELEAYGAIFQSTADTEVIIHLIAHSRQDSVVARIIDALG
                     KVRGAFSLLFLCSEGLIAVRDPFGVRPLSVGRLREGWVVSSESVAFNLVEADYVRDIE
                     PGELVVINDRGLTSYRPFAPTRQAHCVFEHVYFSRPDSRVFGHNVYTVRKRNGIRLAK
                     ESPVAADVVIPVPDSGIPAALGFAEGSGVPFEEGLVRSHYIGRTFIEPQDSIRHFGVR
                     IKLSPIREVLEGRRVVVVDDSLVRGTTSRKLLKMLRGAGAKEVHLRIASPPIISPCFY
                     GIDTPTRQELIATSHTVEETRKYIGADSLAYLSREGLFSAFGDQEAGGASFCDACFTG
                     AHPVPFTKEELVQLGLF"
     gene            4398..4880
                     /locus_tag="AC16BC_04830"
     CDS             4398..4880
                     /locus_tag="AC16BC_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3240975.1"
                     /translation="MGADTRYFHGGQVREIVGLTRRQMQYWDRTGLVTPGLRTQGGHA
                     RYTFEDLVAYKTAKKLLDAGVSLQRIRKVMTRIREILPTIKRPLVELTLVATGDVVLV
                     FYERTVFEAITGQEWLLDIADVQRDVDRWQRNVDTIKRYRRRSSMGSPVSPDAAAHQG
                     "
     gene            5051..5773
                     /locus_tag="AC16BC_04835"
     CDS             5051..5773
                     /locus_tag="AC16BC_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240976.1"
                     /translation="MAVTALAACLARRGRRVLVGQTHEQDFGVVFGLCGRTPPEPSGV
                     VVTSDLGVRLSRMPLVGVRRPGVLIDGALKTQWDACVGEADVVVIHVNHDGGILWTQE
                     IPCPDEFVTVAGAAAHASLLRSYEAIKRVVYRNSDAVLRLATTDPDGRAESRWRGVAR
                     AVETFLGRQCLMDSAFHHVSAMASAFLAGRLCRDGGGQVIRTMESIMARWGERASPRA
                     NRSELPGYGAPWGIISAREPAR"
     gene            5770..6351
                     /locus_tag="AC16BC_04840"
     CDS             5770..6351
                     /locus_tag="AC16BC_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240977.1"
                     /translation="MRQDPMFRPSGHQATELPGAPDIGALVYLAAAHNPVIRPEAVIE
                     RTVVGDGVGLLVGEEEGRTTLLYLWWQERPDFLAWMAHVARWTSLSPTWRKAQGLKAS
                     DVPIRVILAVPSVSEGLRDAVTLLSPSVTVLRYRWEARGGACALRWDEDSATVCSPAG
                     DRVGGATPAGIFSSASADEALTPEERDFFQQFA"
     gene            complement(6373..6837)
                     /locus_tag="AC16BC_04845"
     CDS             complement(6373..6837)
                     /locus_tag="AC16BC_04845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3240978.1"
                     /translation="MFGATVPLDHGTYQQALQMETCRSIRYAHFFSVCHIGIDQDDLT
                     TEVPFCSVATIVRETIRETDLIGLTNGQTISILLLHADIQSAYQIAERIRSRIALQTF
                     VNERHPLKVTASIGGACFPTHGNDAQTLLLRADEMLALAQRNGGNQTSVPDI"
     gene            complement(6893..>8106)
                     /locus_tag="AC16BC_04850"
     CDS             complement(6893..>8106)
                     /locus_tag="AC16BC_04850"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3240979.1"
                     /translation="LNRLQSDLAILIILPQEHSHFQKLIAASPKLRVLTEPVDLTMFS
                     QRILEATGLSTPLDRVIGQDELRNQGDYSHLLGLSPKMKEIMMIINQVAPTNIKVLIR
                     GESGTGKELVARTLYARSLRKDKPFIKVLCAALPEGLLESELFGYEKGAFTGALRRKP
                     GKFEFANHGTIFLDEIGEISPGLQAKLLQVLQDGEFSRVGGETDVKVDTRVLAATNKN
                     LEKAVADGTFREDLFYRINVVSVFLPPLRERKEEIPYLVEYFLEKFNHQYNKRYPRLS
                     ENTLHRFLQYEWPGNVRELENIVKRIVVLDNEDIVFDKAPAKEEGPTHIPSALPTPAA
                     GSTASSSPPVSSYSLKSVGKEAASQAERDLIRNILYQTHWNRKRAAELLQISYKALLY
                     KIKKYDLNNAG"
BASE COUNT         1369 a         2316 c         2733 g         1688 t
ORIGIN      
        1 gggtcgcggg ggactcacac tcgggatttg caacggcttt cagatcctgg tcgaatccgg
       61 cctgttgccc ggggccctct tgcgcaacgc ctcgttgaag tttatttgtc gaaccgtggg
      121 ggtgcgggtt gagcggaccg atacgccctt tacctcccga tatgtcgtcg ggcaagtctt
      181 acgactgccg atcgcgcatg cggacggcgc gtactgggcc gacgcggaca ccctggctcg
      241 cctgcgggat caggggcaaa ttgtgtttcg gtatgcgccg ctcgacgatg gcgtggccaa
      301 ccccaacggg tcgatcgacg ccatcgccgg catctgcaac gagacccgga acgtgctcgg
      361 catgatgccg cacccggagc gcgcgtccga gtcggcgctc ggcggatacg atggccgtgc
      421 gttgtttgaa tcggcgctcg gcgcgctctg cccgaccgcc taaatcgacg cgactctgca
      481 tgtcactgga acacgtgctt tccgaaccgt tggaccactc gttgctcacc gggcacgggg
      541 tgaccgcgga cgaataccgc cgttttcggg agcgattggg gcgggcgccc acgttggtcg
      601 agctcggcat cctgtccgtg atgtggagcg agcattgcag ttataagagc tctcgggtcc
      661 atcttcggcg gttccccacg accggggacg aggtcgtcca aggccccggc gagaacgcgg
      721 gcgccgttga tatcggcgac gggttggccg cggtcttcaa gatcgaaagc cacaaccatc
      781 cgtcgtttat cgagcccttc caaggggcgg cgaccggggt cggcggcatc ttgcgggacg
      841 tcttcacgat gggcgcgcgc ccgatcgcgc tgctggattc gcttcgtttc ggtccgttgg
      901 acgagccgcg cacgaaatat ctgtttgagc gcgtggtcgc cgggatcgcc ggatacggca
      961 attgcgtcgg ggtgccgacg gtgggcggcg aggtggcgat ccaggatctg tatcgctcca
     1021 atccgctcgt caacgtgttc tgtctcggcc tggcccgcaa ggatcggctc ttcctcggtc
     1081 gcgcagccgg cgtgggcaac ccggtcatct acttcggcgc caaaacaggt cgtgacggga
     1141 ttcacggcgc gaccatggcc tcggaagcgt tcggtgacgg ggcctccgag aaacggccga
     1201 tggtgcaggt cggcgacccc tttatggaga agttgctgat tgaagcgtgt ttggagctca
     1261 tggccgggga tctgatcgtc gggatccagg acatgggcgc ggcggggctg accagttcct
     1321 cctgcgaaat ggcggggcgc gctggcaccg gggtggagat cgacgtggcg ctcgtcccga
     1381 cgcgcgagac cgggatgacg ccgtacgaga ttatgttgtc cgagtcgcag gagcgcatgc
     1441 tgctggtggc caaaccgggc tgtgaggaga aggtccgagc ggtgtgcgag aagtgggatt
     1501 tggatgtcgc ggtgatcggg cgcgtgaccg acaccagccg gctggtcgtg cgccgcggcc
     1561 aggcgatcgc ggcggacctt ccgatcgacc tcttgaccga cgatgcgccg aagtacgagc
     1621 ggccgatcgc gccgccgccc tatctcgaca gtctccaggg actctcgctg gaggcgcttc
     1681 ccgagcccaa ggactacgcc gaggcgctgc tgaccgtgtt gggttccccg accgttgcga
     1741 gcaaggcgtg ggtgtatcgt cagtacgatc atatggtcgg aaccaattcg atcgtgctcc
     1801 cggggtcgga cgccgccgtg ctccgcgtca agggcaccga cacggcgctg gcggtgagcg
     1861 tggacggcaa cagccgttat tgcatgttga acccctacgt cgggggcatg atcgccatcg
     1921 ccgaggcctg ccgcaatgtg gtgtgcagcg gcgcccggcc attgggtgcc agcgactgtc
     1981 tgaatttcgg gaacccggag cggcccgaag tgatgtggca gtttgcgttg gcggtcgacg
     2041 ggatgtcgga ggcctgccgc gcgttcgggg tgccggtggt cagtgggaac gtcagtttct
     2101 acaacgagac ccgggggatc ggcatctacc ctacgccggt catcggcgtg gtgggcaaag
     2161 cgccggtcga gaaggtcacc acgcaggcgt tcaaggcgtc cggtcgcgcg gtgattttgc
     2221 tgggagaaac caaagaagag ttggggggat cacagtacct ggcgacgatc catcaccgcg
     2281 aacaggggct cccgcccgaa ctggtcctgg aggccgaact ggccttgtac cggtgcgtgc
     2341 tcgaagtcac cggtgagggg ttggtagagt ccgcgcacga ttgctcggac ggcggcttgg
     2401 ccgtggcgct ggccgaatgc tgcgtatcgg cggagtcgtc ggtgggggca cgcgtcgcgt
     2461 tgggcaatcc ggggatgatc cgccgggacg cgatcttgtt cggcgagagc caatcccgtg
     2521 tcgtgctgtc ggttaaaccg gatcacgccg agcgtgtgct cgccgtggcg gcccagtggg
     2581 gagttccggc ggctcggatc ggggagaccg gcggcgatcg gctgtcgatt gccgtggacg
     2641 gtgacgcgtt ggtggacgtc gagttgtccg ctgtccgctc cgcgtggagc gacggcttgt
     2701 cgaaccaatt gggggcctcc gcgtgcgcgt cgaggtgatg ccgttcgata aaatgcgcga
     2761 agaatgcgga attttcgggg tgtacggtca tcccgaggcc gcgaacctca cgtatttggg
     2821 gttacacgcg cttcagcacc gcggccaaga gggcagcgga atcgtctcgt cggacggccg
     2881 cgactactac gtggaaaaag gcccgggttt ggtgctcgaa gtgttcgaca aatcccggat
     2941 ggcccgcttg cccggtcgga tggcgatcgg ccacaaccgg tattcgaccg cgggcggctc
     3001 gcacgaaaaa aaccttcagc cgtttctgat caacttcgcg cgtggtcacc tggcgctggc
     3061 gcacaacggc aacctcgtca acgcccaagg cctgcgcagc gagttggaag cctacggggc
     3121 tattttccag tccaccgcgg ataccgaggt cattattcat ctcatcgcgc attcccgaca
     3181 ggattcggtg gtggcgcgaa tcatcgacgc gttggggaaa gtccggggcg cattcagcct
     3241 cctcttcctc tgttccgagg gactgatcgc ggtacgcgat cccttcggcg tccggccgct
     3301 gtcggtggga cggctccggg agggatgggt ggtgtcgtcc gagtccgtgg ccttcaactt
     3361 ggtcgaggcc gattacgtcc gggacatcga accgggtgag ttggtggtca tcaacgaccg
     3421 ggggttgacg tcgtatcgcc cgtttgcgcc gaccagacag gcccactgtg tgtttgaaca
     3481 cgtgtatttt tcgcgtcccg atagtcgggt cttcggccac aacgtgtata cggtccgcaa
     3541 gcgaaacggt atccggttgg cgaaggaaag tccggttgcc gcggatgtgg tgattccggt
     3601 tccggactca ggcattccgg cggcgctcgg gtttgccgag ggatcggggg tgccgttcga
     3661 ggaaggattg gtcagaagcc attacatcgg ccggacgttt atcgagccgc aggactccat
     3721 tcgccacttc ggggtgcgga ttaaactcag cccgattcgg gaagtgctgg aaggccgtcg
     3781 ggtggtggtc gtggacgatt cgctggttcg cggcacgacg agccggaagc tgctcaaaat
     3841 gttgcgcgga gcgggcgcca aggaggttca cttgcgcatc gcgtcgccgc ccatcatctc
     3901 gccctgtttc tacggcatcg acacgccgac gcgccaggaa ctgattgcca cgtcccacac
     3961 cgtggaggag acccgaaagt acatcggggc ggacagtctg gcgtatctca gccgcgaagg
     4021 gctgttttcg gcgttcggag accaggaggc aggcggggcc tcattctgcg acgcgtgttt
     4081 taccggtgcc catcccgttc ccttcaccaa ggaagaactc gtccaactcg gcttgttctg
     4141 aaacggcgca tcaccgaacc gttcaaaagc cgtcagagtt gtatcactcc ttgacagcgg
     4201 agatacccct tgattaagat ggccccacaa acggggtctt gacgtaccag gagccggggt
     4261 gtgagtgccg tgctcggtgc acggcgcagt cgtggagtgc ccaagggttc ccggtgtctt
     4321 ctttcccttt gttcgggata cgttgcaggg aacgcggcgt gctcccgacc cggtaacccg
     4381 ttccgtgagg tccgctcatg ggtgctgaca cgcgttactt tcatggtggc caagttcggg
     4441 agatcgtcgg actgacccgc cgccagatgc agtactggga tcgtaccggt ctagtcaccc
     4501 cgggactgcg cacacaaggt ggacacgcgc ggtatacgtt tgaagacctt gtggcctaca
     4561 aaacggccaa gaaactcctt gacgccggcg tgtcgttgca gcgtatccgg aaggtgatga
     4621 ccaggattcg tgagatcctg ccgaccatca agcggccgct tgtcgagctg acgctggtgg
     4681 ccacgggaga cgtggtgttg gtcttctatg agcgcacggt gttcgaggcc atcaccggtc
     4741 aggagtggtt gctcgatatc gcggatgtcc aacgcgacgt ggatcgctgg cagcgcaacg
     4801 tcgacacgat caagcgatat cggcgtcggt cctcgatggg gtccccggtg tctcctgacg
     4861 cggcggccca tcagggctga gcgtccggat tatgcaacga cggcttgaag acgtctcgca
     4921 ttttttctta ggggacgcga aggccgttgt ccccagccgc cgctcagacc cttccctcac
     4981 acaagcggtc cgactcgacg gaccatggac ggtttatctc gcaagccgtg aggatcacgc
     5041 gcgcgcggcg atggcggtca ccgccctcgc ggcgtgtctg gcgcgtcgcg gtcgtcgagt
     5101 cttggttgga cagacgcatg agcaggattt tggcgtggtc ttcggtctgt gcggacggac
     5161 ccccccggag ccctcgggtg ttgtggtgac gtctgacctt ggcgtgcggc tgtctcgcat
     5221 gccgttggtc ggcgtccgcc gcccgggggt gttgatcgac ggggcgttga aaacgcagtg
     5281 ggatgcctgc gtcggcgagg cggacgtggt ggtgattcac gtcaatcatg acggcggcat
     5341 tttgtggacg caggagattc cgtgtccgga tgaattcgtg acagtggccg gagccgccgc
     5401 ccatgcgtcg ttgctccggt cctatgaggc gatcaagcgt gtcgtctatc ggaattccga
     5461 tgcggtgctt cgtctggcca cgacggatcc tgacgggcga gccgaatcgc gatggcgggg
     5521 agtggcgcgg gcggtggaga cgtttctcgg ccgtcagtgc ctgatggaca gcgcttttca
     5581 ccacgtgtcg gcgatggcgt cggcgttcct tgcgggccgg ttgtgccgtg atgggggggg
     5641 gcaggtcatc cggaccatgg agtcgatcat ggcccgttgg ggcgaacgcg catccccgcg
     5701 ggccaaccga agcgaattgc ctggttatgg cgcgccgtgg ggaatcatct ccgcccgcga
     5761 gccggcgcga tgaggcagga tccgatgttc cggccgtcag gtcaccaggc aaccgagctt
     5821 cccggggcgc ctgatatcgg agcgctggtg taccttgcgg ccgcccacaa tccggtcatc
     5881 cgcccggagg ccgtcataga acggaccgtg gtcggggatg gtgtcgggct cttggtcggt
     5941 gaagaggagg ggcggacgac gctgctctat ctctggtggc aagagcggcc ggattttctg
     6001 gcgtggatgg ctcatgtcgc acggtggacg tcgctgtccc caacctggag aaaggcgcag
     6061 ggcttgaagg cgtccgacgt tccgatccgg gtcattcttg cggtcccgtc ggtcagcgag
     6121 gggttacgcg atgccgtcac gttgttgtcg ccgtcggtga cggtgctgcg gtatcgatgg
     6181 gaggctcgtg gcggcgcgtg cgccttacgg tgggacgagg attccgctac ggtctgctcg
     6241 ccggcagggg atcgagtagg aggagcgacg cccgccggca tcttttcttc ggcctcggcc
     6301 gacgaggctc tcactcccga ggaacgggat ttttttcagc aatttgcgta gccagaatac
     6361 ttcgtcggat tctcagatgt ccggtacgga cgtttgattg cctccattgc gttgcgcgag
     6421 cgctaacatt tcgtcggccc gcagcaacag cgtctgcgcg tcgttcccgt gggtcgggaa
     6481 gcaggcccca ccgatactcg ccgtgacttt gagtggatgc cgttcgttca cgaacgtctg
     6541 gagggcaatc cgactccgga tgcgttccgc gatctgatac gcgctttgaa tgtcggcatg
     6601 gagcagaaga atggaaatcg tttgtccgtt ggtgagtccg atcaaatcgg tttccctgat
     6661 ggtttcgcgg acgatcgtgg cgaccgaaca gaacggaact tcggtggtca ggtcgtcctg
     6721 gtcgatcccg atgtggcata ccgagaagaa gtgggcgtac cggatcgagc ggcacgtctc
     6781 catctgaagc gcctgctgat aggttccgtg atcaagcgga actgtcgcac caaacatggt
     6841 aactcctcct tggtctcacc atgttcccca tcagcctgtc gggcgccgct ggtcagcccg
     6901 cattgttcag gtcgtatttc ttgatcttat agaggagtgc cttgtagctg atctggagca
     6961 gttccgccgc gcgcttgcga ttccagtgag tctggtacag aatattacgg atcagatcac
     7021 gctcggcctg gctggcggcc tccttgccca cgcttttgag cgaataactc gacaccggag
     7081 gtgacgacga agccgtcgac ccggccgcgg gcgtggggag agccgacggg atgtgcgtcg
     7141 gcccctcttc tttcgccggc gccttgtcga agacgatgtc ttcgttgtcg agcacgacga
     7201 ttcgtttcac gatattttcc agttctcgaa cattgcccgg ccactcatac tgcaagaaac
     7261 gatggagcgt attttcagaa agcctcggat agcgtttgtt gtattggtgg ttaaactttt
     7321 ccaaaaagta ctccacgagg tacggaattt cctcttttcg ttccctcagg ggagggagaa
     7381 aaacagacac cacgttgatg cgatagaata ggtcttcgcg aaacgtccca tccgcgacgg
     7441 ccttttccag atttttgttg gtggcggcaa ggacgcgggt gtcgaccttc acgtccgttt
     7501 ctccgccgac ccgggagaat tctccgtcct gtaaaacttg cagtaacttg gcctggagcc
     7561 ccgggctgat ctctccgatt tcgtcgagaa aaatcgtccc gtgattcgcg aactcgaatt
     7621 tcccgggttt acggcgaagc gccccggtaa acgcgccctt ttcgtacccg aacagttcgc
     7681 tttccagtag gccttccggg agcgccgcgc acagtacctt gataaagggt ttgtccttac
     7741 ggagtgatcg tgcatacaaa gttcgcgcca caagctcttt ccccgtgccg ctttctcccc
     7801 gaataagcac cttgatgttg gtcggggcga cctgattaat gatcatcatg atctctttca
     7861 tcttgggact caggccgagg aggtggctgt agtcgccttg attccggagc tcatcttgtc
     7921 cgatcacacg gtcaagcggt gttgaaaggc cggtggcttc gagaatgcgc tggctaaaca
     7981 ttgttaaatc aacgggctcc gtgaggacgc ggagcttggg agaggccgcg ataagctttt
     8041 ggaaatggct gtgttcttgg ggtaggatga ttaaaatcgc taagtcggac tgtaaccggt
     8101 taagcc
//
LOCUS       DBNGTT010000217         4723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_427984, whole genome shotgun sequence.
ACCESSION   DBNGTT010000217 DBNGTT010000000
VERSION     DBNGTT010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_427984"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="AC16BC_04855"
     CDS             complement(<1..153)
                     /locus_tag="AC16BC_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240980.1"
                     /translation="MRVLVSDSLSTQGVEILKQAGLDVDVKTKLTPEQLLDEIKNYEG
                     LVVRSAT"
     gene            complement(176..1312)
                     /locus_tag="AC16BC_04860"
     CDS             complement(176..1312)
                     /locus_tag="AC16BC_04860"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3240981.1"
                     /translation="MKKKYLLAPGPTAVPPEILLAMAQPMVHHRSKDFEPIVASVKED
                     LKWLFQTTQDVMILASSGTGGMESAIANVLSPGDKVIAINGGKFGERWVKMCQAYGLT
                     VDEVKVEWGTAVDPAAIEQRLKKDPAKAVFVQASESSTGVAHDVKALGAIVKNFPNTL
                     LVVDAVSAMGVFDLKTDEWGLDIVVTGSQKALMLPPGLAFVSLSEKAWASAEKAALPK
                     FYFNLKRERDNIRKNTTAYTPAISLIIGLQKAFELLKPEGLDGIFARHGRLARATREA
                     MKAIGLELFAKGSPSDALTAVNAPQGVDAEAVYKTLRDKYGITTAGGQDHLKGKIFRI
                     SHMGYADTFDVITAVAAVEMVLKGMGHSVTLGKGVGVAQGILVQ"
     gene            complement(1383..2378)
                     /locus_tag="AC16BC_04865"
     CDS             complement(1383..2378)
                     /locus_tag="AC16BC_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3240982.1"
                     /translation="MLGKNILFGVLSLTLLGLFGFVVYQQENPEWEHYQREYYQILSQ
                     KMGDPKLAATPVKLQQIWMPDLNRTDRCMNCHMGIGNPAFADQPQPFKSHPFLDTYMK
                     AHKFEKFGCTVCHDGDGQATVVERTHGIVHHLDRQLATGVMVQSACARCHIDMHEPGV
                     DYPEAPAVMWGKQFVTQTAFMKCGACHAVKQYGWSATAAPEISGIGSRTELSFYLVHD
                     FQHLQSPIHTKARWEFEHFLDPQKITPGKKQINKETGQEEWVGRTIMPNFVSIHKLTE
                     EQTWMLTAWVMSLRDPKVEKIPIAYLPPHRQPAAPPVEAAKPAPSTKPTPAKRKA"
     gene            complement(2552..3382)
                     /locus_tag="AC16BC_04870"
     CDS             complement(2552..3382)
                     /locus_tag="AC16BC_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HIR3240983.1"
                     /translation="MADQSQTPVSTAGAKKSYGLSDLVKGSAPGATKPPEEMVFTWPH
                     LVYVEFIAMLAATIVLIVLGLISGAPLEESASADTTPNPMKAPWYFLGLQELLVFFDP
                     WLAGVVLPGLIIVGLMVVPYIDINPKGIGQYNYRDRKFAVWTFSFGLALWYIFIVIGV
                     YTRGLDWQWYWPWDDWTLHKEASAVALIDLEIIFSQKLGISQALANKLTYVFTLLFYG
                     VGFTIPFLFYRKFYESLGFVRYNVMMLFMMTMMLVPFKVMIRLVFNIKYMLITPWFKI
                     "
     gene            complement(3456..4223)
                     /locus_tag="AC16BC_04875"
     CDS             complement(3456..4223)
                     /locus_tag="AC16BC_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR3240984.1"
                     /translation="MGWFEKLQQQIVNTQVWRSVFRHGYPDTDINRALAVFTNVILHL
                     HPVKVKRGALKIRYTWCLGGLTFFMFIVLTITGVFLMFYYVPDTRRAYNDIKDLQYVV
                     YLGNLMRDMHRWAAHAMVFLVWLHMTRVFLTGSYKPPREFNWVVGVVLLVLTLILSWT
                     GYLLPWDQLALWAITVGAKMAEATPVIGVAGPFGLQLGMRPDNDIRFMLLGGTVVGQS
                     ALLRFYVLHCVGLPLLAALFMAVHFWRIRKDGFSGPL"
     gene            complement(4318..>4723)
                     /locus_tag="AC16BC_04880"
     CDS             complement(4318..>4723)
                     /locus_tag="AC16BC_04880"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR3240985.1"
                     /translation="YEPPTVFKIGKPTDYGMGVTDTLKKERQIWVVRNEKGLYVLVAI
                     CRHLGCTPNWFQDQARFRCPCHGSIYDIFGNVRGGPAPRTLWRAAVSLDPVDGQVVVN
                     FIQRQDPDPQSTVDGLTVPEEGREVEPFFVKV"
BASE COUNT          985 a         1510 c         1306 g          922 t
ORIGIN      
        1 cgtggcgcta cggacgacca gcccctcgta gttcttgatt tcatccagca actgttcggg
       61 cgtcaacttg gttttgacgt cgacgtcgag accggcttgc ttgagaatct ccaccccttg
      121 ggtggacaga ctatcgctga cgagcacacg catagtgttt aagtggctga gaatcctatt
      181 gcaccaaaat cccctgagcg accccgacgc ccttcccgag cgtcacggag tgccccatcc
      241 ctttcaacac catctccacg gccgcgaccg cggtgatcac gtcgaacgtg tccgcgtacc
      301 ccatgtggga gatccggaaa atcttcccct tgagatgatc ctgtccgccc gccgtggtaa
      361 tgccgtactt gtcccgcaac gtcttgtaca cggcttcggc gtcaacgcct tggggcgcgt
      421 tcaccgcggt aagcgcgtcg ctcggcgagc ccttggcgaa caattccagt ccgatcgcct
      481 tcatcgcctc gcgcgtggcg cgcgccaacc gaccgtgtcg agcgaaaatc ccgtccaagc
      541 cctcgggttt gagcagctca aacgccttct gaaggccgat gatcagcgaa atcgccggcg
      601 tataggccgt ggtgtttttc cggatgttgt cccgctcacg ctttaaattg aagtaaaatt
      661 tcggcagcgc ggccttctcc gccgacgccc acgccttctc gctcagcgag acgaacgcca
      721 agcccggcgg caacatcagg gctttctgcg aaccggtcac gacgatgtcc aggccccatt
      781 catcggtctt gaggtcgaag acgcccatcg ccgacaccgc gtcgaccacg agcagcgtat
      841 tcggaaagtt tttcacgatc gcccccagcg ccttcacgtc gtgggccacg ccggtcgaac
      901 tctcgctcgc ttgaacgaac acggccttcg ccggatcctt cttgaggcgc tgctcgatcg
      961 cggcgggatc gaccgccgtt ccccattcca ccttgacttc atcgacggtc agcccgtacg
     1021 cctggcacat cttgacccaa cgttcgccga acttcccgcc gttgatcgca atgactttgt
     1081 cgccgggcga caggacgttg gcgatcgcgc tctccatgcc gccggttccg ctcgacgcca
     1141 ggatcatcac gtcctgggtc gtctggaaca gccacttgag atcctctttg acggacgcca
     1201 cgatcggctc gaagtccttc gagcgatgat gcaccatggg ttgcgccatc gccaggagga
     1261 tttccggcgg cacggcggtc ggaccgggag caagcaggta tttctttttc atacgttacg
     1321 actccgaaat cagcgagggc gacaccctac cattagcttt ccgcgcgagt caagaaaccg
     1381 gattacgcct tccgcttggc gggcgtcggc ttggtcgacg gcgcgggctt cgccgcttca
     1441 accggcggcg cggcaggttg gcgatggggc ggcagatagg cgatggggat cttctcgacc
     1501 ttgggatcac gcagactcat cacccaggcg gtcaacatcc acgtctgttc ctcggtcagt
     1561 ttgtggatgg acacgaagtt gggcatgatc gttcgcccga cccactcctc ttgacccgtt
     1621 tccttgttga tttgcttttt gcccggcgta atcttctgcg gatccaagaa atgttcaaac
     1681 tcccaccgcg ctttggtgtg gatcggcgat tgcagatgct gaaagtcatg gacgagataa
     1741 aacgacaatt cggtccggct cccgatcccg gaaatctccg gcgcggccgt ggctgaccag
     1801 ccgtactgtt tgaccgcgtg gcaggcgccg cacttcatga acgccgtctg ggtcacgaac
     1861 tgcttgcccc acatcacggc cggcgcctcc gggtaatcca cgcccggctc gtgcatatcg
     1921 atatgacacc gggcgcaggc cgactgcacc atcacgccgg tcgccaattg acggtccagg
     1981 tgatggacga tcccatgggt ccgctccacc accgtggcct ggccgtcgcc gtcatgacac
     2041 acggtgcagc cgaacttctc aaacttatgg gccttcatat aggtgtcgag gaacgggtgc
     2101 gacttaaacg gttggggctg gtccgcaaac gccggattgc cgatgcccat atgacagttc
     2161 atgcagcggt cggtccgatt gaggtccggc atccagatct gctggagctt caccggggtg
     2221 gccgccaact tgggatcccc catcttctgg gacaggatct ggtaatactc gcgctgataa
     2281 tgttcccact cagggttctc ctgctggtac acgacaaagc cgaagagccc gagcagcgtc
     2341 agactcagta cgccgaataa gatgtttttt cccagcatcg atgcgcgact ccggataagg
     2401 gagaggggac cgtcgttatt tcttgaacga ccgctccagc cacatggcaa tcccgacgaa
     2461 caacagcgtg atgagggccg tgcccaccac aataaaggcc ccgatctcta aattttcttt
     2521 gctcatggac gagccctctt tctgccgcgc gtcagatctt gaaccacggc gtgatcaaca
     2581 tgtacttgat gttgaacacc agtcgaatca tcaccttaaa ggggaccaac atcatggtca
     2641 tcatgaacag catcatcacg ttgtaccgca cgaagcccaa cgactcgtag aacttcctat
     2701 aaaacaaaaa cggaatggtg aacccgactc cgtaaaacaa cagcgtgaag acgtacgtca
     2761 gcttattcgc caaggcttgc gagatgccca gtttttgcga aaagatgatt tcgaggtcga
     2821 tcaacgccac cgccgaggct tctttgtgca aggtccagtc gtcccagggc caataccact
     2881 gccagtccaa gccgcgggta tacacgccga tcacgatgaa gatgtaccag agcgcgagac
     2941 cgaacgagaa cgtccacacc gcgaatttac ggtcccggta gttgtactgc ccgatcccct
     3001 tcggattgat atcgatgtac gggacgacca tcagcccgac gatgatcaac ccgggcaaca
     3061 cgacgcccgc caaccacgga tcgaaaaaga ccagcagctc ctggagcccg agaaagtacc
     3121 acggagcctt catcgggttg ggcgtggtgt ccgcgctggc cgactcctcc aagggcgccc
     3181 cggagatcaa tcccaacacg atgaggacga tcgtcgcggc caacattgcg ataaactcga
     3241 cgtagaccag atgcggccac gtaaagacca tctcctccgg aggcttcgtc gccccgggtg
     3301 ccgacccttt caccaggtcc gacaagccat aggacttttt cgcgcctgcg gttgaaaccg
     3361 gcgtttgtga ctgatcagcc atagcgactc ctcacctcgc agccaacatc gaaacggcgt
     3421 aacaacgcat cgtggtccga gcgattgacg ggggattaca gcggccccga aaacccgtcc
     3481 ttccggatgc gccagaagtg cacggccatg aaaagcgccg cgagcaacgg cagcccgacg
     3541 cagtggagca cgtagaatcg caacaacgcg ctctgcccca ccacggtgcc gccgagcaac
     3601 atgaaccgga tgtcgttgtc cggccgcatc cccaactgaa gaccgaacgg tcccgccacg
     3661 ccgatgaccg gcgtggcttc ggccatcttc gcgcccaccg tgatggccca caacgccagc
     3721 tggtcccagg gcaagagata tccggtccac gacaagatca gcgtcaggac gagcagcacc
     3781 accccgacca cccagttgaa ctcgcgcggc ggcttgtacg acccggtcag aaacaccctc
     3841 gtcatatgaa gccacacgag aaagaccatg gcgtgcgcgg cccagcgatg catgtcgcgc
     3901 atcaggttgc cgagatacac cacgtattgg agatctttaa tgtcgttata cgcccggcgg
     3961 gtatcgggca cgtagtaaaa catcaggaac acgccggtga tggtcaggac gatgaacatg
     4021 aaaaaggtca atcccccgag acaccacgtg tagcggatct tgagcgcgcc tcgctttacc
     4081 ttcaccgggt gaaggtggag gattacgttg gtaaacacgg cgagggcgcg gttgatatcg
     4141 gtgtcgggat atccgtgtcg aaacaccgat cgccacactt gagtattgac gatctgttgt
     4201 tgcagctttt caaaccaacc catcatgcgt cccccttcac cgagcttgcc gagtgcctcg
     4261 caggccctgc tctcccgggc cgagaagcga gctaattagg gcttccaacc accgcgccta
     4321 gactttgacg aagaacggtt cgacctcgcg cccctcttcc gggactgtca ggccgtccac
     4381 cgtggactga ggatccgggt cctgccgctg gatgaagttg acgaccacct ggccgtcaac
     4441 cgggtccaag ctcacggccg cgcgccacag ggttcgaggc gccggcccgc cgcgcacgtt
     4501 gccgaaaatg tcgtagatgc tgccgtgaca cgggcagcgg aagcgcgcct gatcctgaaa
     4561 ccagttcggg gtgcacccca aatggcggca gatcgccacc aacacgtaga gtcccttttc
     4621 gttgcgcacg acccagattt ggcgttcctt cttgagcgta tcggtcaccc ccatgccgta
     4681 atccgtgggt ttgccgatct tgaagacggt cggcggctcg tac
//
LOCUS       DBNGTT010000218         4875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_811912, whole genome shotgun sequence.
ACCESSION   DBNGTT010000218 DBNGTT010000000
VERSION     DBNGTT010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_811912"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="AC16BC_04885"
     CDS             <1..902
                     /locus_tag="AC16BC_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015386 - potassium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HIR3240986.1"
                     /translation="LIGLGFGMAAAWAINRIRLESSGLYPVMTLAIGLGTFGFANTVG
                     GSGFVAVYLAGLTIGQRVTVSQRLILDFHDGLAWLMQIVMFVALGLLSFPSRLWTAAD
                     QGLILSAVLMVVARPVAVFISLLSSRFSLRERLLISWVGLRGAVPIILATYPILAGLP
                     NGPVIFNLIFFVVVTSVLLQGTTISWAARALGLHSHRRPEPRHRLHITSIEQTDRRIV
                     DYYLDPKSPAVGRLIKDLAMPHETFIAMLVRAGQVLAPKGHTRLEAGDHLFVLSRKRD
                     RLDINQVFDEGPGASAGSPAPSP"
     gene            972..2792
                     /gene="uvrC"
                     /locus_tag="AC16BC_04890"
     CDS             972..2792
                     /gene="uvrC"
                     /locus_tag="AC16BC_04890"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3240987.1"
                     /translation="MTHVLQRVAEAPHAPGVYVMRGAGGRILYVGKAKSLPDRVRSYV
                     QPGGRLAPKTAALVAHVQDIEYFVTGSELEALILENNLIKKHRPKYNIVLRDDKHYPF
                     IRLAMQDAYPRPEIVRRVARDGALYFGPYVPTNALRETLRVLRYAFPLPNCSITIDGT
                     ADRACIELEIGRCLAPCTGRQSQSDYHEMIEQVRLFLEGRDHELTRALESRMAAAADA
                     LRFEEAARIRDQLGRIRKVLERQRITSTRLEDQDVVALVREGESADVQVLFVRGGMLI
                     GRKDFYWAQAEPDDAALVTAVLQQVYDRDTVVPPEIVLPVEVPDDALLASWLSARRGG
                     RVRLITPSRGPDARLLELARQNADAAMGDHRRRERTGEVEARELQTVVSLAARPRRIE
                     AFDISNFQGDQAVGSMVVWEDGKMKKADYRKFAVRTIEGANDFGMLYEVVTRHYARAQ
                     ARNGPWPDLVVIDGGRGQLSAAVDALRALDIQVGDGGLAVIGLAKERGEKGERVFLPG
                     ASDPILLDPTAASTRLLQRIRDEAHRFAITYHRAVRHRRLAQSRLDAVVGIGPVKKKA
                     LLARFGSVERLAEASEADVRSVPGMTDVLARLVLRALRAR"
     gene            complement(2942..3898)
                     /locus_tag="AC16BC_04895"
     CDS             complement(2942..3898)
                     /locus_tag="AC16BC_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240988.1"
                     /translation="MADIFYIPLVALRHQWTKEYELGQGFELVRFPSAVAEAYREFDH
                     GAFRKRHGDVLLREIQANYCLKISWPIEPFKNAREMVYHLEDPAITLLIVLRIIKPTS
                     AGLRTMIHVPATLELGMPFDFVDYRMQGFAMRRPEDQPVFTDDDVPTIVYYYAKLLEL
                     LKREYHHRRIFNALRFFDLGYRSENLDSRLIYFSIALEVLFKPLRGRISRGMTDRIVD
                     FLGTTPTERDAITRTVLAFIDLKARITHGDMTYFDITRAENVTLVREQEDLLRAVLQK
                     ILQKDKLIQIFSQVRERDSYFDQRLGPPPPAVRRPAPGNAPA"
     gene            complement(4004..4402)
                     /locus_tag="AC16BC_04900"
     CDS             complement(4004..4402)
                     /locus_tag="AC16BC_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3240989.1"
                     /translation="MAEQTVAVTTETKGISAQEDLRAPERHLTPAVDIFETSSGLIVV
                     ADLPGVHRDGLDLRVDDDVLTIQGRPRVESTGDERYREYTLYPFFRQFELTESVDQNR
                     IAAHLQHGVLTLTLPKAEKAPPRRIPGQIG"
     gene            complement(4457..4672)
                     /locus_tag="AC16BC_04905"
     CDS             complement(4457..4672)
                     /locus_tag="AC16BC_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3240990.1"
                     /translation="MLRRTDGLRPRDPRRALHSKRAAGKFSRSMDLPVDVDDRRVAAH
                     YEHGVLRITLPKAESAKPRRITVAVAA"
BASE COUNT          827 a         1497 c         1629 g          922 t
ORIGIN      
        1 cgttgatcgg tctcggattc ggcatggccg cggcgtgggc gatcaaccgg attcgcttgg
       61 aatcctcggg cctgtatccg gtgatgacgc tggcgatcgg cctcggcacg ttcggcttcg
      121 ccaacacggt ggggggaagc ggatttgtgg cggtttacct ggccggcttg accatcgggc
      181 agcgggtcac ggtttcccag cgattgatcc tcgattttca cgacggcttg gcgtggctga
      241 tgcaaatcgt gatgtttgtc gcgctgggac tgctctcatt cccttcccgg ctctggaccg
      301 cagcggatca aggactcatc ctgtcggcgg ttctgatggt ggtcgcccgt ccggtggccg
      361 tattcatctc gttgttgtcc tcccgttttt ccctccgcga gcgcctgctg atttcgtggg
      421 tcggcctgcg cggggcggtg ccgatcattc tcgccaccta tccgatcttg gctggcctgc
      481 ccaatggccc ggtgattttc aatctcattt tcttcgtggt cgtaacctcg gtgttgctgc
      541 agggcaccac gatctcgtgg gccgcgaggg cgttggggct tcacagccac cgacggcccg
      601 agccgcgcca tcgcctccac atcacgtcga tcgagcagac cgaccgccgc atcgtcgact
      661 actacctgga tccgaagagt cccgcggtcg gccgcttgat caaggatctc gccatgccgc
      721 acgaaacgtt catcgcgatg ctcgttcgcg ccggccaagt gctggcgccc aagggccaca
      781 cgcgcctgga ggccggggac catctctttg tgttgagtcg caaacgagac cgcctggaca
      841 tcaaccaagt ttttgacgag ggacccggcg ccagcgccgg ctccccggct cccagtccct
      901 gaatcaaggc gccgctggcc ggcacgcgcg attgatcaag cccccgcggc ggacgtagaa
      961 ttccgtcagt catgacgcac gtgttacagc gggtggccga agctccgcac gcaccgggcg
     1021 tgtatgtgat gcgcggggcg ggcggtcgga ttctgtacgt ggggaaggca aaatccctgc
     1081 cggaccgcgt gcggtcctac gttcagcccg gcggccggct ggcgcccaaa accgccgcgc
     1141 tcgtcgccca cgtgcaggac atcgaatatt tcgtcaccgg gtccgaactc gaggcgctga
     1201 tcctcgaaaa caatctgatc aagaaacacc gccccaagta caatatcgtc ctccgggacg
     1261 acaaacacta ccccttcatc cgcctggcca tgcaggacgc ctacccgcgg cccgagatcg
     1321 tgcggcgcgt cgcccgtgac ggcgccctct acttcggtcc gtacgtgccg accaacgccc
     1381 tgcgtgagac gctgcgggtc ctccggtacg cgtttccatt gccgaactgt tcgatcacga
     1441 tcgacgggac tgcggatcgc gcgtgtatcg agctggaaat cggtcgctgc ctcgcccctt
     1501 gcacggggcg acaatcgcag tcggactacc acgagatgat cgagcaagtc cgcttgttcc
     1561 tggagggccg cgatcacgag ttgacgcgcg cgctggagtc ccggatggcg gccgcggccg
     1621 acgcgctacg gttcgaggag gcggcgagaa ttcgcgatca gctcggacgc atccgcaagg
     1681 tcctcgagcg ccagcgcatc acctcgacga ggctggagga tcaggacgtc gtggcgctcg
     1741 tgcgcgaggg cgaatccgcg gatgttcagg tgctcttcgt gcgcggcggg atgttgatcg
     1801 gacggaagga cttctactgg gcgcaggccg agccggacga cgcggccttg gtcaccgcgg
     1861 tgctgcaaca agtctacgac cgggacacgg tggtgccccc ggagatcgtg ctgccggtcg
     1921 aggtgccaga cgacgcatta ctggcgtcgt ggctctcggc gcgtcgcggc gggcgggtgc
     1981 gattgatcac gccgtcgcgg ggaccggatg cgcgattgct cgaattggcc cgccagaacg
     2041 cggacgccgc aatgggcgac catcgacgac gcgagcgcac cggagaagtc gaggcgcgcg
     2101 agctgcaaac ggtggtgtcc ctggccgcga ggccccgccg catcgaagcc ttcgacattt
     2161 ccaattttca gggcgaccag gcggtggggt cgatggtggt gtgggaagac ggcaagatga
     2221 agaaggcgga ctaccgcaag ttcgcggtca ggaccatcga gggcgccaat gatttcggga
     2281 tgctgtacga ggtggtgacc cggcattacg cccgtgcgca agccaggaat ggaccgtggc
     2341 cggatttggt ggtgatcgat ggcggcaggg ggcaactttc ggcggcggtc gacgccctgc
     2401 gtgcgctcga tatccaagtg ggagacggcg gcctcgccgt catcgggctt gccaaggagc
     2461 ggggggagaa gggcgagcgg gtcttcctgc ccggcgcgtc ggacccgatc ctccttgatc
     2521 cgaccgcggc gtcaacgcgg ctcctgcagc gcattcgcga tgaagcccat cggtttgcga
     2581 ttacctacca ccgggcggtt cgtcatcgcc gcctcgcgca gtcgcggttg gatgcggtgg
     2641 tcgggatcgg cccggtgaaa aaaaaagcgc tgctggccag gttcggcagc gtggaacgcc
     2701 ttgccgaagc ctcggaagcg gacgtcaggt ccgtgccggg gatgacggat gtgttggcgc
     2761 ggttggtcct gcgcgccctg cgcgcccgtt aagaggccgt ccaggaacgg tcatctgcgg
     2821 cgttgccgct gcgcatccgg tgctcacgta ctcacaacgt acgctccgct tcggagctcg
     2881 cggcgccttg catctgacca ctcctgaacg gcctcacaat ggccgaatac caggggaaat
     2941 tctacgcggg ggcgttgccg ggcgccgggc gtcgcaccgc cggtggcggc gggcccagtc
     3001 gttgatcgaa gtacgagtcg cgctcgcgga cctgggagaa aatctgaatc agtttgtctt
     3061 tctggagaat tttctgaagc acggcgcgga gcaggtcttc ctgctctcgg accagggtca
     3121 cgttttcagc ccgcgtgatg tcgaagtagg tcatatcgcc gtgggtgatg cgtgctttca
     3181 ggtcgatgaa cgcgagcacg gtgcgggtga tcgcgtcgcg ctcggtggga gtcgtcccca
     3241 aaaaatcgac gatacggtcg gtcatgccgc ggctgatccg cccgcggagg ggtttaaaca
     3301 acacttccag cgcgatggag aagtaaatca ggcgcgaatc caggttctcg ctccggtacc
     3361 ccagatcgaa aaaacgcaac gcgttgaaga tgcggcggtg gtggtactcg cgtttgagga
     3421 gctcgaggag cttggcatag tagtacacga tggtcggcac gtcgtcgtcg gtaaacaccg
     3481 gttggtcctc ggggcgccgc atggcaaaac cctgcatccg gtaatccacg aaatcgaacg
     3541 gcatcccgag ttcgagggtc gcggggacat ggatcatcgt acgaaggccg gcgctcgtgg
     3601 gcttgatgat ccgcaatacg atcaacaacg tgatcgcggg atcttcgagg tggtacacca
     3661 tctcccgcgc gttcttgaac ggttcgatcg gccacgagat tttcaggcaa tagttggcct
     3721 gaatctcgcg cagcagcaca tcgccgtggc gcttccggaa ggcgccgtga tcgaactccc
     3781 gatacgcttc ggccacggcc gacgggaacc ggaccagttc gaacccttgg ccgagttcgt
     3841 actccttggt ccactggtgc cgaagcgcga cgagcggaat gtagaaaatg tcggccatct
     3901 ggggtgctat gcgtttgagg gatagcgccg cgcacactgt accagaaagc cgcggtgaaa
     3961 ctcaaagccg ggccggcgat cgtggatcgt cggcccggcc gcgtcagccg atctgccccg
     4021 gaatccggcg gggtggggct ttttccgcct tgggcagcgt gagcgtcaac acgccgtgct
     4081 ggagatgcgc cgcgattctg ttttggtcca ccgactccgt cagttcaaac tgtcggaaga
     4141 acggatagag cgtgtattcg cgataccgct cgtcgccggt ggattccacg cgcggtcggc
     4201 cttggatggt gaggacgtca tcgtccacgc gcaggtccag cccatcccgg tgaaccccgg
     4261 gcagatctgc gaccacaatc agccccgagg acgtctcaaa gatgtccacc gccggcgtga
     4321 ggtggcgctc cggcgctcgg aggtcttctt gcgcgctgat ccctttggtc tcggtcgtga
     4381 ccgcaaccgt ttgctccgcc acggagtggt ctcctttcgt tctggctatg cgatgttcgc
     4441 cttcgttatg ccgctgttat gccgccaccg cgacggtgat tcgacgcggt ttggcggatt
     4501 cggctttggg caatgtgatc cggagcacgc cgtgttcata gtgcgcggcc acgcgtcgat
     4561 cgtccacgtc aaccggcaga tccatggatc ggctgaactt gcccgcggca cgtttgctat
     4621 ggagtgcgcg tcgggggtca cggggtcgga ggccatcagt ccgacggagt ataagcccga
     4681 cacgggcggc cgggcaatgg ggttacccca ttacaccaac cgcagccgag gaggcggagg
     4741 cgtgcgaagc atggcgttga ccggacccag aaagatcgcg ccgatatcgt gcgggggacg
     4801 gccgtaacgc agtcgcgctt ttctgagcag gtccagataa ttgcggcccc gctcgcgacg
     4861 ccggcgttct accac
//
LOCUS       DBNGTT010000219         4156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_300515, whole genome shotgun sequence.
ACCESSION   DBNGTT010000219 DBNGTT010000000
VERSION     DBNGTT010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_300515"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="AC16BC_04910"
     CDS             <1..412
                     /locus_tag="AC16BC_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIR3240991.1"
                     /translation="RNAPDVHQPWTPEQWRTLQAYLRGTDLINRWYFWEAHEAWEPLW
                     RAHPPQSDPARFIQGLIGATAALLKHRMGNERSAQTLSTAACERLSSFRGIWMGLAVD
                     AFRTDLDRYVASITAGASRPFGPETPRIRLTTMA"
     gene            637..2091
                     /locus_tag="AC16BC_04915"
     CDS             637..2091
                     /locus_tag="AC16BC_04915"
                     /inference="COORDINATES: protein motif:HMM:NF017591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR3240992.1"
                     /translation="MQSHGNRWKAALLAPVLIGMVGFSAHVDKRADDEHDFGVGVERM
                     LEDQSKRLFGFREPLQESAPETTGPYRTASQRASDQVLVARGLDVEYLTRTAANATDM
                     MAFYPAADPSHLITCVEGGRQTLASGKLNPSVQRIHLRTGEVETVLRGMTSCDGIRLT
                     PWGTVLATEETSDGGAYEILDPLTTTEQTVLDRATGLVTDSGHIAKRTALPTISWEGI
                     AVLPSGVIIAGDELRPGTSIVDSDGGAIFKFIPTAPRTSAGPINTLNESPLTSGSVRA
                     LQVLVSNNQFGQGGEIGRGRWIVVNAATARRDADAGGATGYYRPEDLHQDPMFADASN
                     PLAVRVCWTDTGNEDFSNYAEVMCGVDANPNAPSSTVIVNRFIEGAADFNSFDNLDFQ
                     PVTGNLYVVEDHDNGDIFACLPDGADRDIKSDGCVKMLSVKDTSAEPTGFLFSTDGTT
                     AYVSIQHSNDANMPLVDGYATDDVIKITGFRVKR"
     gene            2166..2531
                     /locus_tag="AC16BC_04920"
     CDS             2166..2531
                     /locus_tag="AC16BC_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3240993.1"
                     /translation="MSDVLTINMDFASGDGAVYRASDLILEVTPTGSGDDVTVTGRVY
                     HPLHGYVDVSTPQPLVTLTGDDYPSSGALVVTGAGNSSARLTALSATTFQIDVDADGD
                     SAYETTVGAFTWGTLEPAV"
     gene            complement(2609..3610)
                     /locus_tag="AC16BC_04925"
     CDS             complement(2609..3610)
                     /locus_tag="AC16BC_04925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR3240994.1"
                     /translation="MTRDPLQARLIPTRESTDGDEFRFALIRTILDASPDGILVVNDR
                     DRIVTHNQRMLQVWDVQGSELPGARDGSAEGIADHHLLSCVLSRVADPDAFLARVKEL
                     YTNPEAEDHCEIELKDGRTLERHSTGLWNQRGKYLDRVWFFRDITSRKRTEAALMDLA
                     QTDPLTGVANLRQFLARANEEFVRAKRFGRQVSIVIVDIDHFKTINDRWGHATGDEVL
                     KTLCRMWTGILRQVDLLARVGGEEFAWLLTETGQKSACNGPTRPCIGPKRTGETAPSP
                     ATDHVFRDATPRVPADTTYVKECGACHFGCQPGLLPECSWRKLFGRRRARGSFWLQW"
     gene            complement(3801..>4156)
                     /locus_tag="AC16BC_04930"
     CDS             complement(3801..>4156)
                     /locus_tag="AC16BC_04930"
                     /inference="COORDINATES: protein motif:HMM:NF046841.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6982 domain-containing protein"
                     /protein_id="HIR3240995.1"
                     /translation="GRIVKGSTGDFFPDKQWFHLTEKDTAAASRVDMAKLKGIFFVKD
                     YHGNPDYESTQDAERKGLGKKVKVCFKDGESIVGYTSGFSPAKPGFFLFPADANGNTE
                     KIFVISVATEEIKFL"
BASE COUNT          787 a         1338 c         1261 g          770 t
ORIGIN      
        1 ccggaatgcc cccgacgtgc accagccctg gacacccgag cagtggcgca ccctgcaggc
       61 ctaccttcgc gggacagacc tcatcaaccg ctggtatttc tgggaagcac acgaggcgtg
      121 ggagccgctg tggcgcgcgc acccgcccca gtccgacccc gcgcggttca tccagggact
      181 gatcggcgcg acggccgcct tgctcaaaca tcgaatgggc aacgagcggt ccgcgcaaac
      241 actctcgaca gcggcttgcg agaggctgtc gtcctttcgc ggaatctgga tgggactcgc
      301 ggtcgatgca ttccggaccg acctcgatcg ttacgtggca tcgatcaccg ccggagcatc
      361 tcggcccttt ggtccggaga cgccccggat acgactcacc accatggcct gatcctgtcc
      421 cggcccgggc cgagttgtcg gtcgacaggg ctctgggttc cgtacccgcg tcaccagcac
      481 actcgcacgc catccacaat ttcattcttc tttaacctga ccgtgacccg gtcgtaaccc
      541 tgagtcgcta gagtgctttc gcaccacatc gcgggcgttt cgggccgcgc gagatttggg
      601 acgaaggacg cacttaccgt cacaccggag gacaccatgc agagtcacgg gaacagatgg
      661 aaggccgcgc tgttggcgcc ggtcctgatc gggatggtcg gcttcagcgc ccatgtcgat
      721 aagcgggcgg acgacgagca tgatttcggc gttggggtcg agcgaatgct ggaagatcaa
      781 tccaaacgat tgttcggttt tcgggagccg ctgcaagagt cggcgcctga aaccaccggt
      841 ccgtatcgca cggcgtccca acgcgcctca gatcaggtcc tggtcgcgag agggctggac
      901 gttgaatatc tgacccgcac cgcggccaac gcgaccgaca tgatggcgtt ctatccggcg
      961 gccgatccca gccacctgat tacgtgcgtc gagggcggac ggcaaacctt ggccagcggc
     1021 aagctgaatc cttcggtgca acgtattcat ctgcggaccg gcgaggtcga aaccgtcttg
     1081 cggggcatga cctcgtgcga cggaattcgt ctcacgccct ggggtacggt cctagccacc
     1141 gaggaaacgt ccgacggagg cgcgtatgaa atcctcgacc cgttgaccac cacggaacaa
     1201 accgttctcg atcgcgcgac cggcctggtg acggattcag ggcacattgc caaacggacc
     1261 gcgttgccga ccatttcctg ggaagggatc gcggtgttgc cgagcggcgt gatcatcgcg
     1321 ggggatgagt tgcggcccgg cacctcgatt gtcgacagcg acggcggggc gatcttcaag
     1381 ttcattccga cggcgccgcg cacatcggca ggcccgatca acaccctcaa tgaatcgccg
     1441 ctgacgtcag ggagtgtgcg cgctctgcag gttctcgtgt ccaacaacca gttcggccaa
     1501 ggcggcgaga tcggccgagg gcgctggatc gtggtcaatg cggccaccgc gaggcgagat
     1561 gccgacgccg gaggagccac cggttactac cgcccggaag acctgcacca ggacccgatg
     1621 ttcgccgacg cgagcaaccc cctggcggtc cgggtgtgtt ggaccgacac gggcaacgag
     1681 gacttctcca actacgccga ggtgatgtgc ggggtggacg cgaatccgaa cgcgccatca
     1741 tccaccgtca tcgtgaaccg gttcatcgaa ggcgccgccg acttcaattc gttcgacaac
     1801 ctcgacttcc agccggtcac cggcaatctc tatgtggtcg aggatcacga caacggagat
     1861 atcttcgcct gcctacctga cggcgcggac cgggacatca aatcggacgg gtgcgtgaag
     1921 atgctctcgg tcaaggacac gtccgcggag ccgaccggtt ttctcttcag cacggacggc
     1981 accaccgcgt atgtgtcgat ccaacacagc aacgacgcga atatgccctt ggtcgacggt
     2041 tatgcgacgg acgacgtgat caagatcacc ggctttcggg tgaaacgctg acgcgatctc
     2101 ctcgaaggcg ctcgacgaaa ccgaggagcg cgggcggacg tggcacgatc ggcgccgcgt
     2161 tcggcgtgtc cgacgtactg acgatcaata tggatttcgc atccggagac ggcgctgtct
     2221 accgtgcgag cgatttgatc ctggaagtca cccctaccgg gagcggcgat gacgttacgg
     2281 tcacggggcg cgtctaccac cccctccacg gctacgtgga tgtatcgacc ccgcaaccgt
     2341 tggtcacgct gaccggagac gactatccat cgtccggagc attggtggtg accggagccg
     2401 gcaattccag cgcccgcctc accgcgttgt cggccacgac gtttcagatc gacgtggacg
     2461 cggacggcga cagcgcctat gaaaccacgg tcggcgcctt cacgtgggga acgttggaac
     2521 ctgccgtctg atggaccgcg tgaaacgcaa gaggggcgcc cggttgggcg cccctctttt
     2581 tcaataaccc gcggctgatc ctttcagatc accattggag ccaaaacgat ccgcgagcgc
     2641 gtcggcggcc aaacaacttg cgccaggagc actccggcaa cagacccggc tgacacccga
     2701 aatggcacgc cccgcattcc ttgacatagg tcgtatcagc ggggacgcga ggcgttgcgt
     2761 cgcggaaaac gtgatcagtc gctggactcg gtgcggtttc gcccgttcgc tttggcccga
     2821 tacagggccg cgtcggcccg ttgcacgcac tcttctgtcc ggtctccgtc aacaaccacg
     2881 cgaactcttc cccgccgacg cgtgccagga gatcgacctg tcgaaggatt ccggtccaca
     2941 tgcgacacag cgtcttgagc acctcgtccc cggtggcatg gccccatcgg tcgttgatgg
     3001 tcttgaaatg gtcgatgtcg acgatgacga tggagacctg ccggccgaac cgctttgcac
     3061 ggacgaactc ttcgttcgcg cgggccagga actgcctgag gttcgccaca cccgtcagcg
     3121 ggtcggtttg agccagatcc atcaacgcgg cttcggtccg tttgcgcgac gtgatgtcac
     3181 ggaaaaacca gactctgtcc agatactttc cgcgctgatt ccacagtccg gttgaatggc
     3241 gctcgagcgt ccgcccgtcc ttcaactcga tctcgcagtg gtcctccgcc tcggggttcg
     3301 tgtataactc cttgacccgg gcgagaaacg cgtcgggatc agccacccgg gaaagcacgc
     3361 acgacaacaa gtggtgatcc gcgatgcctt cggccgatcc gtcgcgcgct cccggcaact
     3421 cggagccttg cacgtcccag acctgcaaca tgcgctggtt gtgcgtgacg atccgatcgc
     3481 gatcgttcac gaccaagatg ccgtccggcg acgcatcgag gatggttcga atcagggcaa
     3541 aacggaactc gtccccgtcc gtcgactccc gcgtcgggat caatcgggct tgcagcggat
     3601 cccgcgtcat cccgacaccg accgcgacgg tcggtccgct ggcagccagt ccgtcatcgc
     3661 gcgcattccc ggtttcacgc cgtcatgtaa gcgagaggtc acgccctacg ctgaaacctc
     3721 gcgttgtggg cgacgcgatg tcgccggtga tcaccgtcgg tccaccggac gctgtgtggg
     3781 actcctcacc gcgtgggtcg ttagaggaac ttgatttcct cggtggcgac gctgatgacg
     3841 aatattttct cggtgttgcc gttcgcgtcg gccgggaaga ggaaaaaccc cggcttggcc
     3901 ggggaaaatc cggacgtata gccgacgatc gactcgccgt ctttaaaaca aaccttcact
     3961 ttttttccca aaccctttcg ttccgcgtcc tgcgtcgact cgtaatcggg atttccatga
     4021 tagtccttca cgaagaagat gcccttgagt tttgccatgt caacgcgact ggcggccgcc
     4081 gtatccttct cggtgagatg aaaccactgt ttatccggaa agaaatcccc ggtgctgccc
     4141 ttgacgatac gcccgt
//
LOCUS       DBNGTT010000220         1854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_428653, whole genome shotgun sequence.
ACCESSION   DBNGTT010000220 DBNGTT010000000
VERSION     DBNGTT010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_428653"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="AC16BC_04935"
     CDS             <1..1326
                     /locus_tag="AC16BC_04935"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3240996.1"
                     /translation="PEHASQTINLLGWMGGDWSVNVITLIALLLFVGAIGKSAQFPLH
                     TWLPDAMEGPTPISALIHAATMVTAGVFLVARLNPIFSLSPMAMDMVAWVGALTAIFA
                     ATIALTQFDIKRIVAYSTVSQLGYMIMACGLGAYTAGMFHLLTHGAFKALLFLGCGSV
                     IHALGGEQDLRRMGALRWAMPITYWTFLAGAIALAGLPPFAGFFSKDEILWKAFSAGG
                     VGIVFWLIGLVTAFLTAFYIFRLVFSVFHGTSHVSKEARHHLHESPAVVTVPLIVLAA
                     LSLAAGWVGIPGEGLNAIGPFLAPVLEPHPAEAHAESHALEYGLMLASVLVAAAGVWL
                     AHLFYVKSPELPGRLAASAAGLYRLSFNKWYVDEIYDKVFVRPTVQMATALWRGFDVL
                     VIDGAVNGVARATRAWGGVLRVMQSGQLQHYALFMAIGAVLVVGVYLLR"
     gene            1499..>1854
                     /locus_tag="AC16BC_04940"
     CDS             1499..>1854
                     /locus_tag="AC16BC_04940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281374.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR3240997.1"
                     /translation="MGDSSFPIVSLVVFLPLIGAVVGLFLRNAEAVRWGALVTTLITL
                     VASLPLWAGFDRGSHAFQFVERAEWIPTFNIEYSVGVDGISLLLILLTTCLSPIVVLC
                     SWRAIDKRVKEFMTAIL"
BASE COUNT          249 a          564 c          637 g          404 t
ORIGIN      
        1 ccggaacacg cgagccagac catcaacctg ctggggtgga tgggcgggga ctggtcggtg
       61 aacgtgatca ccctgattgc gttattgctc tttgtgggcg cgatcgggaa atccgcgcaa
      121 ttcccgctgc acacctggtt gccggacgcg atggaaggtc cgaccccgat ctcggccctg
      181 atccacgccg ccaccatggt caccgccggc gtgttcctgg tggcgcggct caatccgatc
      241 ttttcgctct cgcccatggc catggacatg gtggcctggg tcggggcatt gaccgcgatt
      301 tttgccgcca ccatcgcgct cacccaattc gacatcaaac gcatcgtggc ctactccacc
      361 gtgagccagc tgggctacat gatcatggcg tgcggccttg gcgcgtacac ggccgggatg
      421 ttccacctgc ttacgcacgg cgcgttcaag gccctgctct tcctggggtg cggcagcgtg
      481 atccacgcgt tgggcggcga gcaggacctg cgtcgcatgg gcgccttgcg ctgggccatg
      541 cccatcacct actggacgtt tctggcgggc gcgattgcgc tggccgggct cccgccgttc
      601 gcgggatttt tctccaagga tgaaattctg tggaaggcgt tctccgcggg cggggtgggg
      661 atcgtgttct ggctgatcgg gttggtcacc gcgttcctga ccgcgtttta catcttccgt
      721 ctggtgtttt cggtgtttca cggcacgtcg cacgtgtcca aggaagcgcg gcaccacctg
      781 cacgagtcgc ccgcggtggt cacggtcccg ctgatcgtcc tggcggcgtt gtcgctggcg
      841 gccgggtggg tgggcattcc cggcgagggc ctcaacgcca tcggcccgtt cctggccccg
      901 gtgctcgaac cgcacccggc cgaggcgcat gccgagagcc acgcgctcga atacggcctc
      961 atgctcgcgt cggtcttggt cgcggccgct ggcgtatggc tggcgcacct gttctacgtg
     1021 aagtctccgg aactgccggg tcggctcgcg gcctcggccg ccgggctgta ccggctctcg
     1081 ttcaacaagt ggtacgtgga cgagatctac gacaaggtgt ttgtccgccc cacggtccag
     1141 atggccaccg cgttgtggcg agggttcgac gtgctggtca tcgacggcgc ggtcaacggc
     1201 gtggcgcggg cgacgcgggc gtggggcggt gtgttgcggg tgatgcagag cgggcagttg
     1261 cagcattatg ccctgtttat ggcgattggc gcggtcctgg tcgtcggcgt ctacctcctc
     1321 cggtgatggg gagcctgtcc atgactggtc atctgcggcg ttgtcgctgc gcgtccgctc
     1381 ctcacgtacg acccagtacg ctgcggtgcg ggcgctcgct ccgccttgca tctgacgcag
     1441 tcctgacagg ctcggcttcg ccggagcttc agagattcaa aggcaatagg aagagatgat
     1501 gggcgattcg agttttccca tagtgtcgct ggttgtcttc ctgccgctga tcggggcggt
     1561 ggtggggttg tttttgcgca acgcggaagc ggtgcggtgg ggggcgctgg tcacgacgct
     1621 gattacgctg gtggcatcgc ttccgctgtg ggccgggttt gatcggggga gccacgcgtt
     1681 ccagttcgtg gagcgggccg agtggattcc gacgttcaat atcgaatact cggtcggggt
     1741 ggacggcatc agcctgctgt tgatcctgct caccacctgt ctctcgccca tcgtggtgtt
     1801 gtgttcgtgg cgcgcgatcg acaaacgcgt gaaagagttc atgaccgcca tcct
//
LOCUS       DBNGTT010000221         4815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2218155, whole genome shotgun sequence.
ACCESSION   DBNGTT010000221 DBNGTT010000000
VERSION     DBNGTT010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2218155"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="bioD"
                     /locus_tag="AC16BC_04945"
     CDS             complement(<1..635)
                     /gene="bioD"
                     /locus_tag="AC16BC_04945"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HIR3240998.1"
                     /translation="MKRGARGVFVVGTDTGVGKTTVAAGLAWALRGRGIDVGVMKPVE
                     TGVRTERGSDAEHLRRAAGVDDPIEAIRPYRFAAPLAPLVAARRERARISPMALTRGY
                     ARLARRHDVMVVEGVGGLMVPLTESKTTLDLAETIGLPLLLVIGNRLGAISHALLTVQ
                     AARSRGLGLLGGVINDLTPKIDLAVRTNPTVLRELLNLPRWAIVPYLTKQAL"
     gene            complement(632..1348)
                     /locus_tag="AC16BC_04950"
     CDS             complement(632..1348)
                     /locus_tag="AC16BC_04950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3240999.1"
                     /translation="MVVITGSSRGLGRSLAQCCGSAGWRVVVHCRERRAEADAVAASI
                     TASGGDALVVQHDVTDPTAFRNIVERAMAAWGRIDAWVNNAGMVDDRLLLSATDATWD
                     RTVSVHLTAAHRAIQAVSPVMHGQGGGVILNVVSLAALQGRRGQAAYSAAKAGSIALT
                     RTAARELAQHGIRVNAVCPPVLDTDAVGPRATTLRRQQLIPDPVSLDGAADAIRAVLT
                     LPWMTGQTIVLDSRIPEGAL"
     gene            complement(1365..2567)
                     /gene="bioF"
                     /locus_tag="AC16BC_04955"
     CDS             complement(1365..2567)
                     /gene="bioF"
                     /locus_tag="AC16BC_04955"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HIR3241000.1"
                     /translation="MRRPSPTTIPDPFAAYRTEIERLRGADRLRTIRAAETGAGPRIN
                     RDGRSLVNFGSNNYLGLAEHPRVKAAAIRAIEREGVGAGASRLLTGDRAVHDELEAAL
                     ARLKGTEAALVFPTGYQANVGVISSLMGRGDLILADRDCHASLIDGCRASGARFRVYR
                     RDRLDQLQAVLARRPETGRALIVTDSVFSMEGDLAPLPELAALADRYDALLLVDDAHA
                     TGVLGLRGTGSSEHWNLRDRPIIQLGTLSKALGALGGFLAGPRILIDYVLNRACTFIY
                     TTALPAAIAAAALEAIRVAGDEPDRRERLWANTRQWRDGVRALGFDTFGSASPIVPLR
                     IGNDGLALRVAAALADEGVYAPAIRPPTVPAGTARLRTSVLATHTPDDLALALSALER
                     AAVRLGVP"
     gene            complement(2568..2873)
                     /locus_tag="AC16BC_04960"
     CDS             complement(2568..2873)
                     /locus_tag="AC16BC_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR3241001.1"
                     /translation="MATSAPALPERDVQPTVEHIPPYKVILLDDPVTTMEFVVNVLTQ
                     VFQKDAATAARLMWEVHTTGASHVATLPLEQAELKQLQVHAAAKAAGFPFRCVIEPA"
     gene            3053..3430
                     /locus_tag="AC16BC_04965"
     CDS             3053..3430
                     /locus_tag="AC16BC_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3241002.1"
                     /translation="MTHQRLAVYQALAAVKSHPTAEEIYASLTPSYPMLSLNTVYTTL
                     ETLKSIGLIQEMRFLDNTARYDANVAPHHHVTCPGCRRVEDFEDAALYRIQPPASVKR
                     RYQLVGRHVQFYGYCPDCRADKH"
     gene            3494..3919
                     /locus_tag="AC16BC_04970"
     CDS             3494..3919
                     /locus_tag="AC16BC_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR3241003.1"
                     /translation="MAKNLQGTKTLANLKEGFAGESQANRRYLYFARKADIEGYPDVA
                     GVFRDTAEGETGHAFGHFDFMAVVGDPVTGVPVGDTDKNLKSAIEGETYEYTQMYPGF
                     AKTAREEGFEEISEWFETLARAEKSHAGRFQKALDSLKK"
     gene            4004..>4815
                     /locus_tag="AC16BC_04975"
     CDS             4004..>4815
                     /locus_tag="AC16BC_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3241004.1"
                     /translation="MEAQFTDPKFWDADATQSEWLRVADICHGCRRCFNLCPSFSILF
                     NGIDALDGEVEQLGAAVSRDVVDACYYCKLCYNHCPYTPTTPHEWDVDFPLLMRRHQI
                     ARAKRDGVPLARKLTTQTDLLGKVASLAPPLMNFANTNRLSRIAMEKTVGIHRDWVQP
                     SYYFETVERWWKRRGGAQGSGENGRAVLFTTCFVNYSDPVAARSAVAVLEHSRVRVDV
                     AYERCCGMPFTDTGDLDSARAAAEANVAMLLPHVEAGALVLAPGPSCSLMLK"
BASE COUNT          840 a         1531 c         1650 g          794 t
ORIGIN      
        1 agcgcctgtt tcgtgagata aggcactatc gcccagcgag gaaggttaag tagctcgcgc
       61 agcaccgtcg ggttggtgcg gacggcgagg tcgatcttcg gggtgaggtc gttgatgacg
      121 ccgccgagga gtccgagtcc gcgcgatcgg gcggcttgca ccgtgagtaa ggcgtggctg
      181 atggcgccca accggttgcc gatcacaagg agcaacggta gcccaatggt ttcggccagg
      241 tccagcgtcg tcttgctctc ggtcagcggg accatcagcc cgccaactcc ttcgaccacc
      301 atcacgtcgt gacgacgcgc gagccgggca taaccccgcg tgagcgccat gggggaaatt
      361 cgggcccgct cacggcgggc cgcgaccagc ggggccaacg gcgcggcaaa ccgatacggg
      421 cgaatggctt cgatcggatc atcaacgccg gctgcacgcc ggaggtgttc ggcgtccgat
      481 ccgcgctctg tccgtacgcc ggtttcaaca ggcttcatca ccccgacgtc gattccacgg
      541 ccacgaagcg cccacgcgag gccggcggcc acggtcgtct ttcccacacc ggtatcagtt
      601 ccgaccacga agacgccgcg cgctccgcgt ttcataacgc gccttccggg attcggctgt
      661 cgagcacgat cgtctggccg gtcatccacg gcagggtgag gacggcgcgg atggcgtcgg
      721 cggccccgtc caatgaaacg ggatccggga tcaattgctg gcgtcggaga gtcgttgcgc
      781 ggggaccgac cgcgtcggtg tcaagcacgg gcgggcatac ggcgttcacg cggatgccgt
      841 gctgggccag ctcgcgcgcg gcggttcggg tgagcgcgat gcttccggct ttggcggcgg
      901 agtaagcggc ttgcccgcgt cgtccttgga gcgccgcgag cgagaccacg ttgagaatga
      961 cgcctccgcc ttgtccatgc atgaccggcg ataccgcttg gattgcgcga tgggctgcgg
     1021 tcaggtgcac cgacaccgtt cgatcccatg tcgcatccgt ggcggacagg agcaagcggt
     1081 cgtcgaccat gcccgcgttg ttgacccatg cgtcgatccg accccacgcc gccatggcgc
     1141 gctccacgat gtttcggaaa gccgtcggat cggtcacatc gtgctgaacc accaacgcgt
     1201 cgccgccgga tgcggtgatc gacgcggcga ccgcatcggc ttccgcgcgg cgctcgcggc
     1261 agtggaccac gacacgccat ccggcagacc cgcagcactg ggccaaggaa cggcccagtc
     1321 cacgcgagga gccggtgatc acaaccaccg ggctgaccgg cgggtcaggg aacgccaagt
     1381 cgaaccgccg cgcgttcgag cgccgacaac gcgagggcca gatcgtccgg cgtgtgcgtg
     1441 gcgaggaccg aggttctgag gcgagccgtg cccgccggta ccgtgggcgg gcggatcgcg
     1501 ggcgcgtaca cgccctcgtc cgccaacgcc gccgcaacgc gcagggcgag cccgtcgttg
     1561 ccgattcgca gcgggacaat gggcgaggcg ctgccgaacg tgtcgaagcc cagggcccgt
     1621 acgccgtcgc gccattgacg ggtattggcc cacaggcgtt cgcgtcggtc cggttcgtcg
     1681 cccgcgacgc ggatggcctc gagcgcggcg gccgcgatcg cggcggggag cgcggtggtg
     1741 tagatgaacg tgcaggcgcg attgagcaca tagtcgatga ggattcgcgg cccggcgagg
     1801 aatccgccga gcgcgccgag cgccttgctc aaggtgccca gttggatgat cggtcggtcg
     1861 cgcaggttcc agtgttccga cgatcccgtg ccgcgcaagc ccaggacgcc ggtggcgtgg
     1921 gcgtcgtcca ccagcaataa ggcgtcgtag cggtcagcca atgccgcgag ctcgggcaga
     1981 ggggcgaggt cgccctccat gctgaagacg ctgtcggtga cgatcagcgc gcggccggtc
     2041 tcggggcggc gcgcgaggac cgcctgcagt tgatccagtc gatcgcgtcg gtagacgcga
     2101 aatcgcgcgc cgctcgcgcg gcaaccgtcg atgaggctgg cgtggcaatc tcggtcggcg
     2161 agaatcagat cgccgcgtcc catcagtgaa gaaatgacgc ccacgttggc ctgatagccc
     2221 gtgggaaaca caagggccgc ctcggtgccc ttgagccgag ccagcgcggc ttccaattcg
     2281 tcgtggaccg cgcggtcgcc ggtcagcaat cgggaggccc cggcgcccac gccttcgcgc
     2341 tcgatcgcgc gaatcgccgc ggccttcacg cgcggatgtt cggcgaggcc caggtaattg
     2401 ttggatccga agttgaccag ggaacggccg tcccgattga ttctggggcc ggccccggtt
     2461 tcggccgcgc ggatcgttcg gaggcggtcc gcgccccgca gtctttcgat ctctgttcgg
     2521 taggcggcga acgggtccgg gatcgtggtg ggagacgggc gacgcattca ggccggttcg
     2581 atcacacagc gaaacggaaa gcccgcggcc ttcgccgcgg catgtacttg cagttgtttc
     2641 aactccgctt gttcgagcgg cagcgtggcg acgtgcgacg cgccggtggt atgaacctcc
     2701 cacatcagcc gggccgccgt ggccgcgtct ttttgaaaca cctgggtcag gacattcacg
     2761 acgaactcca tggtcgtcac cggatcgtcc aaaaggatga ccttatacgg cgggatgtgt
     2821 tcgaccgtgg gttggacgtc gcgctcagga agcgcgggcg ccgatgtagc catagtcgat
     2881 ttcgtaccat atcggcaaac atcgggtcaa acgatcggcg gtcttgacga cgggccatag
     2941 gaatggtaaa tactaggcag taatagttac taatcactgg cattcgatga aatcaaaatc
     3001 tacaaacgat tcgggtgagg ttacgaggcg tctcaaggac gccggtctaa agttgacgca
     3061 ccagcgtctg gccgtgtatc aggcgctggc ggcggtgaaa agtcatccga ccgccgagga
     3121 aatatacgcg tcgctcacgc cgtcgtaccc gatgttgtcg ctcaataccg tgtatacgac
     3181 tttggaaacg ctcaagtcga tcgggttgat tcaggaaatg cgtttcctcg ataataccgc
     3241 gcggtacgac gcgaacgtag cgccgcatca ccacgtgacg tgtcccggct gccgccgggt
     3301 cgaggatttc gaagacgccg cgctctaccg catccagccg ccggcttccg tgaagcggcg
     3361 gtatcaactg gtcggccgcc acgtgcagtt ttacgggtac tgcccggatt gccgggccga
     3421 taagcactga aagatcggcg gagactcgga ggctccgtct gatattcgtt ctcgatcaac
     3481 caagggaggc actatggcga agaatctcca aggcaccaag acgttggcga atctcaaaga
     3541 agggtttgcg ggcgagtcgc aagcgaaccg gcgttatctg tatttcgcgc gcaaggccga
     3601 catcgaaggc tacccggatg tggccggggt gttccgcgat acggcggagg gcgagaccgg
     3661 ccacgcgttc ggccacttcg acttcatggc cgtggtgggc gatccggtga cgggcgtgcc
     3721 tgtcggcgac accgacaaga acctcaagtc cgcgatcgag ggcgaaacct acgagtacac
     3781 gcagatgtac ccggggttcg ccaagaccgc gcgcgaagaa gggttcgagg aaatctccga
     3841 gtggttcgaa acgctggcac gcgcggaaaa atcccatgcc gggcgtttcc agaaggcgct
     3901 cgacagcctg aagaaataac acgggccgac ccgacaccgg gacgtgtgtt ggggcggcgc
     3961 gggccgggac ttcgcgtccc gcgccgccat cacgcgagga gccatggagg cccagtttac
     4021 cgacccgaag ttttgggatg ccgacgccac gcagtccgaa tggctgcgcg tcgcggacat
     4081 ctgtcacggc tgcaggcgct gttttaacct gtgtccgtcg ttttccatcc tgttcaacgg
     4141 gatcgacgcg ctcgacggcg aggtcgagca acttggggcc gcggtctcgc gcgacgtggt
     4201 cgatgcctgt tactactgca agctctgtta caaccactgc ccgtacacgc cgaccacccc
     4261 gcacgagtgg gacgtcgact tcccgctgct gatgcgccgc caccagatcg cgcgggcgaa
     4321 gcgcgacggc gtgccgctgg cgcgcaagct caccacgcag accgacttgt tggggaaggt
     4381 ggcgtcgctg gcgccgccgc tgatgaactt cgccaacacg aaccgcctgt cgcgcatcgc
     4441 gatggagaag accgtcggca tccaccgcga ctgggtgcag ccgtcctact acttcgagac
     4501 ggtcgagcgc tggtggaagc ggcgcggcgg cgcgcagggc tccggcgaga acgggcgcgc
     4561 cgtgctgttc acgacctgct tcgtgaacta cagcgacccg gtcgccgcgc gcagcgcggt
     4621 cgccgtgctc gagcactcgc gcgtgcgcgt ggacgtcgcc tacgagcgct gctgcggcat
     4681 gcccttcacc gacacgggcg acctcgactc cgcgcgcgcc gccgccgagg cgaacgtggc
     4741 gatgctgctg ccccacgtgg aggcgggggc gctggtgctc gcgccgggcc cgtcctgctc
     4801 gctgatgctg aagga
//
LOCUS       DBNGTT010000222         3680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_940519, whole genome shotgun sequence.
ACCESSION   DBNGTT010000222 DBNGTT010000000
VERSION     DBNGTT010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_940519"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..777)
                     /gene="mpqE"
                     /locus_tag="AC16BC_04980"
     CDS             complement(46..777)
                     /gene="mpqE"
                     /locus_tag="AC16BC_04980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083447688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:1901663 - quinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-plastoquinone biosynthesis
                     methyltransferase MpqE"
                     /protein_id="HIR3241005.1"
                     /translation="MLRTPTLPAPAEKERVVQRMFSAIARWYDLNNTVLSLGLHHAWK
                     REAVALTEVKPGGRVLDLCAGTCDLAILLADAVGPAGRVVASDLNPEMLGVGLHKVRA
                     RGLRDRVACALGNAEALQFPDNSFDALTVAFGLRNVTHLDKALAEIHRVLKPGGRAVC
                     LEFSRPTNAAFRKTYDFYSFTLLPKVGTWISRDHTGVYQYLPDSIRHFPAQEPFADLW
                     RKTGFREVSYRNRSGGIVAIHVGVK"
     gene            complement(1170..2909)
                     /locus_tag="AC16BC_04985"
     CDS             complement(1170..2909)
                     /locus_tag="AC16BC_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR3241006.1"
                     /translation="MTPSRSPEPDRATRPALHSTQQVSPGRYPESLTRHLIQLQRDGF
                     LANLRQRRTDLWNPDPAHQAVAANRLGWVDPLSLIELHAAQLTDWAREARARHDAFVL
                     LGMGGSSLAPEVIARVAAAPADAARFTMLDSTDPDAIAAIVARTEAGAPLYLVSSKSG
                     GTVETRSHAEFFFAREAALRPDPGRSFVAITDPDSPLARLSRDRKFAHVFLNAPDIGG
                     RYSALSLVGLVPAALLGLAPSTIAERVSAMMAACAPDAPTYENPALVLGALMAVSALN
                     NADKLTLVTSPALAPFAAWVEQLIAESTGKDGRGIIPVAGEALDRVTTGNDRCVVALT
                     LQGDHDPTLTKTLDALRASHTPLVEIVLPDRLAVFGEFYKWEVATAVAASLLKVDPFD
                     EPNVKESKDMTAAILAQVEKTGRLPIGPVAAETDRLAIWSFAPQPSVEAVLRAFFAAV
                     KPPMYVSVLGYLNPGPGVELAIARLRQAIGAARGVPTLFGWGPRYLHSIGQLYKGGPP
                     SGAFLILTTDHAAAQAIPNAPYTFGQLELAQALGDLEALAKHTRPAIRVHLKGDLAAA
                     LADLEKAINAALK"
     gene            complement(3044..3343)
                     /locus_tag="AC16BC_04990"
     CDS             complement(3044..3343)
                     /locus_tag="AC16BC_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3241007.1"
                     /translation="MGMRLYIGNLSFAASDSDLHALFTQAGQVSSAKVITDVYTGRSR
                     GFGFVEMASREDGQRAIDQFNGKPLKGRPIIVNEARPPAKRTGTDAGARGTSATA"
BASE COUNT          568 a         1164 c         1343 g          605 t
ORIGIN      
        1 tctccttgca gggcagccag acgtacagga tcgacttgat cgcggtcatt tcacgcccac
       61 gtggatggcc acgatcccgc ccgagcggtt gcggtacgag acctcgcgaa acccggtttt
      121 ccgccacagg tccgcgaacg gctcttgcgc cgggaagtgc cggatcgagt ccggcagata
      181 ctggtacacc ccggtgtggt cgcggctgat ccaggtgccg accttgggca acagggtgaa
      241 cgagtagaag tcgtaggtct tgcgaaacgc ggcgttggtg ggccgtgaaa actccagaca
      301 cacggcgcgg ccgccgggtt tgagcacccg gtggatctcg gccagggcct tgtccaggtg
      361 cgtgacgttg cgcagcccga acgccacggt gagcgcgtcg aacgagttgt cgggaaactg
      421 cagcgcctcc gcgttgccca gcgcgcaggc cacgcgatcg cgcaggccgc gcgccctgac
      481 cttgtgaagg cccacgccca gcatctccgg gttcaaatcg ctcgccacca cccggccagc
      541 cggccccacc gcgtccgcga ggaggatcgc gaggtcgcac gtgcccgcgc acagatccag
      601 cacgcgccca ccgggtttga cctcggtcag ggccacggcc tcgcgtttcc acgcgtggtg
      661 caggccgagc gacaggaccg tgttgttgag gtcgtaccag cgcgcaatgg cggagaacat
      721 gcgctggacc acgcgctctt tttcggccgg ggcggggagg gttggggtgc gtagcatagg
      781 cggttgtcgt agcatcattg gcttactggt gtcaatgtgc cggtcgctgt ctggccgcgg
      841 agaaacggcc atctgcggcg ttgtcgctgc gcgtccggtg ctcacgtacc tatttcgtac
      901 gccccgctcc ggtgttcgct ccgccttgca tctgtccatt tctccgcggc cagccgctga
      961 cgcgggttca gtatgtcgtg attcccgcgt tcgcggggat cacggtacct cagaattaaa
     1021 ttatcggtgt cgaataatcc cccccccttg acaaaggggg aaaggggggg ggatttgaaa
     1081 cgctcggggt gatcaccggc tttgcggggg tcacgagcaa gggggttgtc ggattgttgt
     1141 cgtcggggcg cacggccggg cgcccgcagt tacttcagcg cggcgttgat ggctttctcg
     1201 agatccgcga gcgcggcggc gaggtcgcct ttcagatgga cgcggattgc cgggcgggtg
     1261 tgtttggcca gggcttccag gtcgcccagg gcctgggcca gttccagttg gccgaaggtg
     1321 tagggcgcgt tggggatggc ctgggccgcg gcgtggtccg tggtgaggat gaggaacgcg
     1381 ccgctcgggg ggccgccttt gtagagctgg ccgatcgaat ggaggtaccg tggcccccag
     1441 ccgaagagcg tgggcacgcc gcgcgcggcg ccgatggcct ggcgcaggcg cgcgatcgcg
     1501 agttccacgc cggggcccgg gttcaagtag ccgagcactg agacgtacat gggcggcttg
     1561 acggccgcga agaacgcgcg cagcacggct tcgaccgagg gctggggcgc aaaggaccag
     1621 atggcgaggc ggtcggtctc ggccgcgacc gggccgatgg gcaagcgccc ggttttctcg
     1681 acctgggcca ggatcgccgc ggtcatgtcc ttgctctctt tgacgttcgg ctcgtcgaag
     1741 ggatccactt tgagcaggct cgcggccacc gcggtggcga cctcccactt gtagaattcc
     1801 ccgaacacgg ccaggcgatc gggcaagacg atttcgacaa gtggcgtatg gctcgcgcgt
     1861 agcgcgtcca gcgtcttggt gagcgtcggg tcgtggtcgc cctgcagggt cagcgccacc
     1921 acgcagcggt cgttgcccgt ggtcacgcgg tccagggctt cgcccgccac cggaatgatg
     1981 ccgcggccgt ccttgcccgt gctctccgcg atcagttgct ccacccacgc ggcgaacggc
     2041 gcgagcgcgg gcgaggtcac gagcgtgagt ttgtccgcgt tgttgagcgc ggacacggcc
     2101 atgagcgcgc ccagcaccaa cgcggggttc tcgtaggtcg gcgcgtccgg cgcgcacgcg
     2161 gccatcatgg cggacacgcg ctccgcgatg gtggacggcg ccaggccgag caaggccgcg
     2221 ggcaccaggc ccaccaacga cagcgcggag tagcggccgc cgatgtcggg cgcgttcagg
     2281 aacacgtgcg cgaatttgcg gtcgcgcgaa agacgggcca acggcgaatc cgggtccgtg
     2341 atcgcaacaa acgatcgacc ggggtcgggc cgcagcgcgg cctcgcgcgc gaaaaagaat
     2401 tccgcgtggg aacgggtttc gacggtgccg ccggacttgg acgagaccag gtacagggga
     2461 gcgccggctt cggtcctggc cacgatcgcg gcgatcgcgt ccgggtcggt ggaatcgagc
     2521 atggtaaacc gggccgcgtc cgccggggcc gcggccacgc gcgcgatgac ctcgggcgcg
     2581 aggctggacc cgcccatgcc gagcagcacg aacgcgtcgt ggcgggcgcg ggcctcgcgg
     2641 gcccagtcgg tcaactgcgc cgcgtgcagc tcgatcagag acaagggatc gacccacccc
     2701 aggcggttgg cggccacggc ctggtgcgcg gggtcggggt tccacagatc cgtgcggcgc
     2761 tgccgcagat tcgcgaggaa cccgtcgcgc tgcaattgaa tcagatgccg ggtcagcgat
     2821 tccggatacc ggccgggcga cacctgttgc gtggagtgca gcgcgggtcg tgtcgcgcga
     2881 tcgggctcgg gcgagcggga tggcgtcatg tcgtcattgt gaatgattca ggatggtttg
     2941 tcaccaggtc gcgccggacg cgcgcgaaac agggatgtct cgaacggcct caacggggag
     3001 gcctgatcgt cccgctcgcg gtcgaaccgc tccggccggg aggctatgcc gtggccgacg
     3061 tgccgcgcgc gcccgcgtcg gtcccggtcc gcttcgcggg cggacgcgct tcgttcacga
     3121 tgatgggccg gcctttgagc ggcttaccgt tgaactggtc gatggcccgc tgaccgtcct
     3181 cccgcgaggc catttccacg aacccgaagc cgcgcgaccg cccggtgtac acgtccgtga
     3241 tcaccttggc ggagctgacc tgcccggcct gcgtgaacag cgcgtggaga tcgctgtccg
     3301 aggccgcgaa cgacaggttg ccgatgtaga gcctcatccc catatgccat cctcctgttc
     3361 gaccgaggtt cggaccccgg gttgtgcggg gcgagcgtgc ggagcgaccc tccgcccggc
     3421 ggtgacggcc tgcgcggtgc gcgcgcgcag gggtggttga tgcggattgg acaaggagaa
     3481 cggacctgct ggatggtgcg ttgtcgaccg catatggcct ccaacggacc gacatccggg
     3541 tggaccgatg tcgggacgtc cacaccagtt tgagacgggc cgatcctagc acaaacgagc
     3601 gatggggtgc tactcaattg agcgaggaag cggcgccggt ccgagtccga gcggttcggg
     3661 ctcagggaga atcacggccg
//
LOCUS       DBNGTT010000223         2382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1195875, whole genome shotgun sequence.
ACCESSION   DBNGTT010000223 DBNGTT010000000
VERSION     DBNGTT010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1195875"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="AC16BC_04995"
     CDS             <1..245
                     /locus_tag="AC16BC_04995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3241008.1"
                     /translation="RQDPHIPREGRALIYNALSEAGAHFQWHEFYAAHAFIRDEGPRY
                     DPAAARICYDMTLELFKRKLGDGDRRVAAASTETKH"
     gene            complement(257..1975)
                     /locus_tag="AC16BC_05000"
     CDS             complement(257..1975)
                     /locus_tag="AC16BC_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3241009.1"
                     /translation="MTPIRTSYFHRGGLEPLLGVTIPERFADVAARFPDHDAVVSLHQ
                     NRRMSYAQLARAIDVLARGLAGIGFQKGERVAVWSTNNIEWLLLQMATARIGAVLVNI
                     NPAYRTRELAYALQRSEVQGLFVIPSFHTSDYIAMLVELVPELTGGSPGQWKSQACPA
                     LRHVVVYNPTAPDRTERPQPGLVTWQEVLAAGDRVTVDQLQAVTAGLDRDDPINIQYT
                     SGTTGFPKAVVLTHHNILNNAYFSAQAMHFTDADRLIVPVPFYHCFGMVLANLLCLSV
                     GACIIIPSEHFEAGQVLRAVEQERGTAIHGVPTMFIAELEHPRFQSFNLSTLRTGIMA
                     GAPCPPALMTRVMEDMHCREILIGYGETEASPITHLTTRDDTLERRTETVGKNLPHQE
                     VKVVDGSTGQTATLGQIGEICFRGYHVMRGYYGDEAATRKTIDAQGWLHSGDLGTMDA
                     DGYVRITGRLKEMIIRGGENIYPREIEDFLFTHPKVAAVAVFGIPDEYFGEQVAVWIQ
                     LHSGEGATEDEIRSFCAGKIAHFKIPHYIRFVEEFPMTVTGKVQKFRMREIALEQLKA
                     KPPKPA"
     gene            complement(2121..>2382)
                     /locus_tag="AC16BC_05005"
     CDS             complement(2121..>2382)
                     /locus_tag="AC16BC_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3241010.1"
                     /translation="PCPTGWEYDPKDSVTIGKLAVSAGVWPLKEYVDGKVVHTRIPHP
                     RTPVEEYLKLQGRFAHLFTPQRNEAALAEIQSTVDRYWAGVG"
BASE COUNT          417 a          762 c          746 g          457 t
ORIGIN      
        1 gccggcagga cccgcacatt ccccgcgagg ggcgggcgct gatttacaac gcgctgagcg
       61 aggcgggcgc gcactttcaa tggcacgagt tctacgccgc gcacgcgttt atccgcgacg
      121 aaggcccccg ctacgatcca gccgcggccc ggatctgcta cgacatgacg ctggaactgt
      181 tcaagcgaaa actgggcgac ggtgaccggc gcgtggcggc cgcgtcaacc gaaaccaaac
      241 attaagacgg tgggattcag gccggtttgg gtggcttggc cttgagttgc tccaaggcga
      301 tttcccgcat ccggaacttc tggaccttcc cggtcacggt catcgggaac tcctccacga
      361 accggatgta gtgcgggatc ttgaagtggg cgatcttgcc cgcgcagaat gaccggatct
      421 cgtcctcggt cgcgccttca cccgagtgca gttgaatcca caccgcgacc tgttcgccga
      481 aatactcgtc cgggatcccg aacacggcca cggccgccac cttcgggtgg gtgaacagga
      541 agtcctcgat ctcgcgcgga tagatattct ccccgccccg gatgatcatt tctttgagcc
      601 ggcccgtaat gcgcacgtac ccgtccgcat ccatggtccc caggtcgccg gagtgcagcc
      661 agccctgggc atcgatggtc ttgcgggtgg cggcttcatc gccgtagtag cctcgcatga
      721 cgtgatagcc gcgaaaacag atctccccga tctggccgag ggtcgcggtc tgcccggtgc
      781 tcccatccac caccttcact tcctgatgcg gcaggttttt ccccacggtt tcggtgcggc
      841 gctccagcgt gtcgtcccgg gtggtgagat gcgtgatggg tgaggcctcg gtctctccgt
      901 agccgatcag aatctcccga cagtgcatgt cttccatcac ccgcgtcatg agcgcgggcg
      961 ggcacggggc gccggccatg atgccggtgc gcagcgtgga gaggttgaag ctctggaatc
     1021 gtgggtgttc gagctcggcg atgaacatgg tgggcacgcc gtggatggcg gtgccgcgtt
     1081 cctgctccac ggcgcgaagg acctggcccg cctcgaagtg ctcgctcggg atgatgatgc
     1141 acgcccccac cgacaggcac aatagattgg ccagtaccat gccaaaacaa tggtagaacg
     1201 gcacgggcac gatcaggcgg tctgcatccg tgaagtgcat cgcctgagcg ctgaagtacg
     1261 cattgttcag gatgttgtgg tgggtgagca ccacggcctt ggggaacccc gtggtgcccg
     1321 aggtgtactg gatgttgatg ggatcatccc gatccagccc cgcggtcacg gcctggagtt
     1381 gatcgaccgt gacccgatcc ccggcggcca acacctcctg ccacgtgacc agcccgggct
     1441 gtgggcgctc ggttcgatcc ggagccgttg ggttgtacac caccacgtgt cgcaacgcag
     1501 gacacgcctg gctcttccac tgacccggcg agccgcctgt gagttccggg accaattcaa
     1561 ccaacatcgc gatgtagtcg ctggtgtgaa agctcggaat gacaaacaga ccctgcactt
     1621 ccgagcgctg cagggcgtag gcgagttccc gggtgcgata cgcgggattg atgttgacca
     1681 acaccgcgcc gatgcgcgcg gtcgccattt gcagcaacag ccattcgata ttgttggtcg
     1741 accagaccgc aacccgctcc cctttctgaa aaccgatccc cgccagcccc cgggccagca
     1801 catcgatcgc gcgggccagt tgcgcgtagc tcattcgacg gttctggtgg agcgagacca
     1861 cggcgtcgtg atccggaaat cgcgcggcca cgtccgcaaa gcgttccgga atcgtgacgc
     1921 cgagtaacgg ctcgagcccg ccgcgatgga aatagcttgt tcggatgggt gtcatggact
     1981 cggtgtgcaa agaacgttct tacgcgtccg accccgtgga gaagggcctg gattcccgcc
     2041 ttcgcgggaa tgacgttgat cggtcacgcc cgtgcaggcg gccgttcctt cggcggccga
     2101 atgctctctg ttcgtcacgt ttacccgacg cccgcccaat acctatccac cgtggactgt
     2161 atctccgcca gcgccgcctc gttgcgttgc ggcgtgaaca ggtgcgcaaa ccggccttgg
     2221 agcttcaaat actcttccac cggcgtacgc ggatggggaa ttctggtgtg gaccaccttg
     2281 ccgtccacgt attctttcaa cggccacacc ccggcggaca cggcgagctt gccgatggtc
     2341 acgctgtctt tgggatcgta ctcccacccg gtggggcagg gt
//
LOCUS       DBNGTT010000224         3232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2729798, whole genome shotgun sequence.
ACCESSION   DBNGTT010000224 DBNGTT010000000
VERSION     DBNGTT010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2729798"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="AC16BC_05010"
     CDS             complement(<1..897)
                     /locus_tag="AC16BC_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR3241011.1"
                     /translation="MSAVLKHLLWASIAVVCAIAFAFVTGLARPEEHVNALWLVVAAA
                     CFYTLAFRFYGRFLGRVVGLDNRRVTPAHRLNDGKNFHPTHRFVLFGHHFAAIAGAGP
                     LLGPVLAAQFGYLPGFLWLVIGAVVAGAVQDFVILVGSLRRNGRSLPQIARDEVGAVT
                     GLATAIAVLFIVVVALAGLGLAVVNALAHNPWGTLTLALTIPIALLMGVYLQRWRPGR
                     VGEVSIIGVVLLLAALILGRPLAATSFGSLFDLGRESLVWWLAGYGFVASVLPVWLLL
                     APRDYLSSFMKVGVVILLAAGVA"
     gene            1018..1521
                     /locus_tag="AC16BC_05015"
     CDS             1018..1521
                     /locus_tag="AC16BC_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241012.1"
                     /translation="MRRCCLLILLASVAWSTTPAAGEPRDQTLRIDVTDSRREITQAI
                     DATLTLRIVRVPHERAPHFGWEIQVVDRPTRGRGTNLLRPQTFSGGPHPSDVLAWLSR
                     DRHFPDDRVLIVPGQPYEIRIRLIDCRTEQIGDAVSFVSGHVEISWRRLDLAALPPFS
                     PSQAGVR"
     gene            complement(1493..2422)
                     /locus_tag="AC16BC_05020"
     CDS             complement(1493..2422)
                     /locus_tag="AC16BC_05020"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3241013.1"
                     /translation="MAGRFGLPAVRRVTAVAGGVINRSFLFELDDGTGWVCRVDVEGG
                     RGKLGREAAVYRWLWQRAPGLPIASAYHVDSARDLIADEYALLPRLSGFNLGADIEHI
                     PDAVRAGLLVQIGALLRRLHALPDPAGDLIEAHHRGQEWRDYCREWFENLLARCETRL
                     GYAPGWAEHARAWMRERIGMLPESPARVFLHGDFHFGNLQYDVLPAPTLSGCFDLEWA
                     WSGHAACDLLHLHEAAVRYPNYEAPLLEGYDALGWPMELVVYRLIQAIGVLGAAAIER
                     PEPLWDLMAWHGAVIENVLKDRAPFTGLLPAKG"
     gene            complement(2659..>3232)
                     /gene="rpe"
                     /locus_tag="AC16BC_05025"
     CDS             complement(2659..>3232)
                     /gene="rpe"
                     /locus_tag="AC16BC_05025"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3241014.1"
                     /translation="LIHIDVMDGQFVPNLTVGPMIVEAANRVTDLPLDVHLMIHDADA
                     LIPDFAEAGSDYLTVHVEACPHLHRTVQLIKEKGVKAGVSLNPATSLSLIEPILPDVD
                     LLLVMSVNPGFGGQSFIPFVLDKLKAARKMIDAIAPHVLLEVDGGIKPENAKALRKAG
                     AEIFVAGSAIFGKRDYAKTIASMRSEAAKA"
BASE COUNT          675 a         1140 c          952 g          465 t
ORIGIN      
        1 cgccaccccg gccgcgagta aaatcaccac ccccaccttc atgaacgacg aaaggtagtc
       61 gcgcggggcg agcagcaacc acaccggcag caccgacgcc acgaagccgt accccgccag
      121 ccaccacacc aggctctcgc gccccagatc aaagaggctc ccgaaggacg tggccgccag
      181 cggacggccc agaatcagcg cggcaagcag caggaccacc ccgatgatcg acacttcccc
      241 gacccgaccc ggccgccatc gctgtaaata cacgcccatc agcaacgcaa tgggaatggt
      301 cagcgccaag gtcaacgtgc cccacggatt gtgcgccaag gcattgacca cggccaggcc
      361 caggcccgcg agcgccacca ccacgatgaa gagcaccgcg atcgcggtgg ccagacccgt
      421 gaccgcgccc acttcgtcgc gggcaatctg cgggagcgaa cggccgttgc ggcgcagcga
      481 cccgaccagg atcacgaagt cctgcaccgc gcccgcgacc accgcgccga tcaccagcca
      541 caaaaacccc ggcaagtacc cgaactgcgc ggccagcacc ggccccaaca acggccccgc
      601 gcccgcgatc gcggcaaagt ggtgcccgaa caggacgaac cggtgggtgg gatggaagtt
      661 cttcccgtcg ttgagccggt gcgcgggcgt gacccggcgg ttgtcgaggc ccaccacccg
      721 ccccaagaac cgaccgtaga atcgaaacgc cagcgtgtaa aaacaggcgg cggccaccac
      781 cagccagagc gcgttgacgt gctcttccgg gcgcgccaac ccggtgacga acgcaaacgc
      841 gatcgcgcac accaccgcga tcgaggccca gagcaggtgt ttaaggacgg ccgacatgta
      901 atttgagatg gtagcggacg ggttgttgca aagtcaaagc agatcggatt tttcctcagg
      961 tttgacgcgc tttgcggcgc cgtgatagcg tccctccggt aaagcgacga gatcacgatg
     1021 aggcggtgtt gcctgctgat tctcctcgcc tcagtcgcgt ggtccaccac gcctgcggcg
     1081 ggcgaaccac gcgatcagac gttacgcatc gacgtgaccg actctcgtcg cgagatcacg
     1141 caggcgatcg acgccaccct cacgctgcga atcgtgcgcg tgccgcacga gcgggctcca
     1201 cacttcgggt gggaaatcca agtggtggac cggccgaccc gaggccgggg aaccaacctg
     1261 ttgcgtcccc aaacattctc tggcgggccg caccccagcg atgtcctggc ctggctgagt
     1321 cgtgatcggc attttccgga cgatcgggtc ttgatcgtcc ccggacaacc ttatgaaatc
     1381 cggatccgcc tcatcgactg ccgcaccgag cagatcggag acgccgtgtc cttcgtctcg
     1441 gggcacgtgg agatcagttg gcgccgtctg gacctggccg cgctcccccc gttcagccct
     1501 tcgcaggcag gagtccggta aacggcgcgc ggtccttgag cacgttctcg atcaccgcgc
     1561 cgtgccaagc catcagatcc cacaacggct cgggccgctc aatggccgcc gcgcccagca
     1621 cgccgatcgc ctgaatcaac cgatacacca ccagctccat cggccaacct aatgcgtcgt
     1681 aaccttcgag cagcggcgcc tcataattcg gataacggac cgcggcctcg tgcaggtgca
     1741 gcagatcgca ggccgcgtgg ccggaccacg cccactccag gtcgaaacaa cccgagagcg
     1801 tcggcgcggg caagacgtcg tactgcaagt tcccgaagtg aaaatccccg tgcaggaaca
     1861 cgcgcgccgg agactcggga agcatcccga tgcgctcgcg catccacgcg cgtgcgtgtt
     1921 cggcccaccc aggcgcatac cccaaccgcg tctcgcaacg ggccagcaga ttctcgaacc
     1981 actcccgaca atagtcgcgc cactcctggc cgcgatgatg cgcttctatc aagtcgccgg
     2041 ccggatcagg caacgcatgc aatcgacgca gcaacgcccc gatctgaacg agcaacccgg
     2101 cgcgcaccgc atccggtata tgctcgatgt ccgcgcccaa attgaatccg gacagacgcg
     2161 gcagcaacgc atactcgtcg gcaatcagat cccgcgcgga gtcgacgtga tacgcgctgg
     2221 caatcggcaa ccccggcgcc cgctgccaca gccatcgata caccgcggcc tcgcggccca
     2281 gcttgccccg cccaccctcc acatctaccc ggcacaccca ccccgtcccg tcgtccagct
     2341 caaacagaaa agagcgattg atcacgccgc ccgccacggc cgtcacccgt cgcaccgccg
     2401 gaagcccaaa tcgtccggcc atcattttcg ccgtaatgag atcaatcatc aatggaagtg
     2461 gtgagggcaa cacggccgcc gagaatgggc cagatgcaag gcggaacgaa gcctcgcgtc
     2521 gcagcgtact gagtcgtacg tgagggcgag tggcgccgcg atgaagtggc gccaagctat
     2581 gagcgccgcc gagacggtga ggcggccgga tacgaagcga caacgcagca gatgtatcgg
     2641 cggccgaaac cgcgaagatc acgccttcgc ggcttcactg cgcatggaag cgatggtctt
     2701 cgcataatcc cgcttgccaa atatcgccga ccccgccaca aagatctccg cgcccgcctt
     2761 gcgcaaggcc ttggcatttt caggcttgat cccgccgtcc acctcgagca acacgtgcgg
     2821 cgcgatcgcg tcgatcatct tgcgcgcggc cttgagcttg tccagcacga acggaatgaa
     2881 cgactggccg ccgaaaccgg ggttgacgga catcacgagc aacagatcca catcgggaag
     2941 aatcggctcg atcagcgaga gcgaggtggc ggggttgagc gagaccccgg ctttcacccc
     3001 cttttctttg atcaactgaa ccgtccggtg caaatgggga caggcttcca cgtgcacggt
     3061 gagataatcg cttccggcct ccgcgaagtc cgggatcagc gcgtccgcgt cgtggatcat
     3121 caggtgcacg tccagcggca aatcggtgac gcggttggcg gcctcgacga tcatcggccc
     3181 caccgtgagg ttgggcacga actgcccgtc catcacatcg atgtggatca gg
//
LOCUS       DBNGTT010000225         3548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3240613, whole genome shotgun sequence.
ACCESSION   DBNGTT010000225 DBNGTT010000000
VERSION     DBNGTT010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3240613"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="AC16BC_05030"
     CDS             complement(<1..1044)
                     /locus_tag="AC16BC_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR3241015.1"
                     /translation="MAIKNEPESGGAGEPEKDRGMEIFRPLSGSPHPPTVTSISISPG
                     GIPKLPISVAEVTVDGIQGDGRAHAKHVKPTRAISLIEEHVVERIRGEGYAVGPGVMG
                     ENLTVQGLDLLALTPGTRLRFSGGVEIELVEPRKPCFVLDAIHPDLKNAVQGRFGYMA
                     RVVTEGVLRVGESIRVLPRVIGAILAGGHSRRMGRPKEGVVLWDGRPMIEHVVEAMQA
                     VCPQVVIVGSCRGWTPEPPIIRLDDRQADLGPLGGIDTLLASGLANGYLVAGCDQPLL
                     TPPMLRLLLNPPDPGVPCFLRAESGAELDPFPGYFPASWVSVARAALERGRRGLRDAV
                     RSGRWEWVMLPETL"
     gene            complement(1034..2878)
                     /locus_tag="AC16BC_05035"
     CDS             complement(1034..2878)
                     /locus_tag="AC16BC_05035"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3241016.1"
                     /translation="MKTIETVRGGGGEGAKEISQHGTPIDLSQSHPDLVGHPLTINGC
                     GAASSNPNRYQQHGFHFTADNCIACHACESACSEKNNLPPHLSFRKVGYLEGGSYPDV
                     LRLNISMACNHCENPVCLKGCPTLAYTKYVEYGAVLQDPDICFGCGYCTWVCPYNAPQ
                     LDPVKGQVEKCNMCVDRLEIGLKPACVSACLSNALDFGVIERLPQGKQQAKLAIPGFP
                     DPSISRPNIRFQQTRSLPGEFRRADGEPLAYRRDQPGVDRFTAAPGGSPAGAEWGLAK
                     LRSREDPLVVFTLLSQAVVGAFMGIVVGPWVGREGGAALSATAHPFAVAVTLWGLFGL
                     QTLGMVMSTLHLGKPQYFYRAMNNLRHSWVSREILTMGTFYHLLGALAAVTAFPGLIA
                     WLPVGLADRVPWLLGWSGVLAGGLGLYSMTRCYRIKARPFWDHWHSGGAFASSAMILG
                     SATVGAIFCPAEVMAGHSPFSISHATAAVLLIGLVLQGIALTAHLRFLSQRGDEAAVS
                     RRLMLTTYGKTYAARWVSWAALVIAAVTLMVVEDPGAPVAWLPAAWAGIWLAAVVHEL
                     VGRALFYVAVVPTTHPGAFFWGNKVFETHARVSGLANMPQVGVVSDGH"
     gene            complement(2875..>3548)
                     /locus_tag="AC16BC_05040"
     CDS             complement(2875..>3548)
                     /locus_tag="AC16BC_05040"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006031795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR3241017.1"
                     /translation="AQRFEAGRRLKFSDVPGEVFDELRAVSAGRPCDYSGMTHDLIEA
                     RRGIQWPCNETAPAGSPRLYTDGRFHHPDGKARLIPLPFVDNNERPDEQYPFWLNSGR
                     VVEHWHTRTKTGKIGNLNKFSPTPYMEINPSAAERLGIRSMEYVRVVSRRSDAVVMAQ
                     LTERVAPDAVFIPMHYHDCVNRLALGLLDPYSRQPAYKQSAVRIEKIQDQTAAAAANL
                     RARSY"
BASE COUNT          653 a         1237 c         1087 g          571 t
ORIGIN      
        1 caacgtctcg ggaagcatca cccactccca gcggccgctt cgaaccgcgt cgcgaagtcc
       61 tcgacggccc cgctccaacg cggcgcgcgc aaccgacacc caggacgcgg gaaagtatcc
      121 cggaaacggg tcgagctcgg cgccgctttc cgcacgcagg aaacagggaa cgccggggtc
      181 cggtgggttc agcagcaacc gcagcatcgg tggcgtcagc agcggttgat cgcaccccgc
      241 caccaggtag ccgttcgcca ggccgctcgc cagcagcgtg tcgattcctc ccaacgggcc
      301 caggtcggct tgtcgatcgt ccaaccggat gatgggcggc tcaggagtcc aaccacgaca
      361 cgagcccacg atcaccactt gcgggcaaac cgcctgcatc gcctccacca cgtgttcgat
      421 catgggacgc ccgtcccaca acaccacgcc ctctttggga cgtcccatcc ggcggctgtg
      481 accgccggcc aggatggccc cgatcacgcg agggaggact cgaatcgact cgccgacccg
      541 cagcacgccc tcggtcacta cccgcgccat gtagccgaac cggccctgga ccgcgttctt
      601 taggtccgga tggatcgcgt cgagcacaaa acacggtttg cgcggctcga cgagctcgat
      661 ctcgaccccg ccggagaatc gcaggcgggt tccgggagtc agcgcaagca gatcgagccc
      721 ctgcaccgtg agattctccc ccatcacgcc cggcccgacc gcgtacccct cgcctcgaat
      781 ccgctcgacg acgtgctcct cgatgagcga gatcgcgcgc gtgggtttga cgtgtttggc
      841 gtgcgcccgc ccgtcgcctt ggatgccgtc gaccgtcacc tcggccactg agatcggtag
      901 cttgggaatg ccgcccggcg aaatgctgat agaagtgacg gtggggggat ggggggagcc
      961 ggagagcggg cgaaaaatct ccatccctcg gtctttctcc ggttccccgg ctcccccact
     1021 ctccggctca ttcttaatgg ccatctgata ccactcccac ctgcggcata ttcgcgagac
     1081 cgctgacccg cgcgtgcgtc tcaaacacct tgttgcccca aaagaacgcg ccgggatggg
     1141 tggtgggcac caccgccaca tagaacaagg cgcgtccgac caattcgtgg acaaccgccg
     1201 ccaaccagat cccggcccac gccgccggga gccacgcgac cggcgccccc ggatcttcca
     1261 cgaccatgag ggtaacggct gcgatcacca gcgccgccca actgacccag cgcgccgcgt
     1321 atgttttgcc gtaggtggtg agcatgagcc gacgcgacac cgcggcttca tccccacgtt
     1381 gactcaggaa tcgcagatga gcggtcagag caatcccttg aagcacgagc ccgatcagca
     1441 gcaccgcggc cgtcgcgtgc gaaattgaaa acggcgagtg ccccgccatg acttcggccg
     1501 gacagaagat cgcgccgact gtggcgctgc ccagaatcat ggcgctggac gcgaacgcgc
     1561 cgcccgagtg ccagtgatcc caaaagggtc tggctttgat gcgataacag cgggtcatgc
     1621 tgtacaggcc cagccccccg gccaggacgc cgctccatcc cagcagccac ggcacccgat
     1681 cagccagccc cacgggtaac cacgcgatca ggccgggaaa cgcggtcacg gccgccagcg
     1741 cgcccaacag gtggtagaac gtgcccatgg tcaaaatttc ccgactcacc cacgagtgtc
     1801 gcaggttgtt catcgcgcga tagaaatatt gcggtttgcc caggtgcagg gtggacatca
     1861 ccatgcccaa tgtttgcaaa ccgaacagtc cccatagcgt gacggcgacc gcgaacggat
     1921 gcgcggtcgc tgacagcgcc gcgcccccct cccggcccac ccacggtccc accacgatcc
     1981 ccatgaacgc gcccaccacg gcctgcgaca gcaacgtgaa gacgaccagg gggtcctcgc
     2041 gcgatcgcag cttggccagc ccccactccg cacccgccgg cgatccgccc ggggccgccg
     2101 tgaagcggtc cacgcccggc tgatccctgc ggtacgccaa gggctcgccg tccgcgcgcc
     2161 gaaactcacc gggaagcgac cgcgtctgct ggaaccggat gttcggccgg gaaatcgacg
     2221 gatccgggaa tccgggaatc gcgagcttgg cctgctgttt cccctgcgga aggcgttcga
     2281 tcacgccgaa gtccagcgcg ttggacagac aggccgacac acacgcgggt ttgaggccga
     2341 tctccaaccg gtccacgcac atgttgcact tctcaacctg acctttaacc ggatcgagct
     2401 gcggcgcatt gtacgggcag acccacgtgc agtaaccgca gccgaaacaa atatccgggt
     2461 cctgtagcac cgcgccgtac tccacgtact tggtgtatgc gagcgtgggg caccctttga
     2521 gacacaccgg attctcgcag tgattgcagg ccatcgagat gttcaaccgc agcacgtccg
     2581 ggtacgaccc gccctccagg taccccactt tgcgaaacga gaggtgcggc ggcaggttgt
     2641 tcttctccga acaggcggat tcacacgcgt ggcacgcgat gcaattgtcc gcggtaaagt
     2701 gaaacccgtg ctgctggtac cggttgggat tggacgacgc cgcgccgcaa ccgttgatgg
     2761 tcagcgggtg cccgaccaga tcgggatgcg attggctgag atcaatgggt gtgccgtgtt
     2821 ggctgatctc tttcgccccc tccccccctc cccccctcac cgtttcgatc gtcttcaata
     2881 cgacctcgcc cgcaggttgg ccgcggccgc ggcggtttga tcctgaatct tctcgatccg
     2941 caccgcggat tgtttgtacg cgggctggcg tgaatacgga tcgagcaggc cgagcgcgag
     3001 ccggttcacg caatcgtggt agtgcatggg aatgaacacg gcatcgggcg ccacgcgctc
     3061 ggtgagctgg gccatgacca cggcgtccga ccggcgcgag acgacccgca cgtactccat
     3121 cgagcggatg cccaggcgtt cggccgcgct ggggttgatc tccatgtacg gcgtagggct
     3181 gaacttgttg aggttgccga ttttgccggt tttggtgcgg gtgtgccagt gctcaaccac
     3241 ccgtccgctg ttgagccaga acgggtactg ttcgtccggc cgctcgttgt tgtccacgaa
     3301 cggcaacggg atcaaccggg ccttgccgtc gggatgatgg aagcggccgt cggtatataa
     3361 ccgcggcgag ccggcgggag cggtctcgtt gcacggccac tgaatcccgc gacgcgcctc
     3421 gatcaggtcg tgcgtcatgc cggaatagtc gcaggggcgc cccgcggaca ccgcgcgcaa
     3481 ctcgtcgaac acctcgcccg gcacgtccga gaacttcagg cgacgtcccg cctcgaagcg
     3541 ctgggcca
//
LOCUS       DBNGTT010000226         1742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_814017, whole genome shotgun sequence.
ACCESSION   DBNGTT010000226 DBNGTT010000000
VERSION     DBNGTT010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_814017"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..461
                     /locus_tag="AC16BC_05045"
     CDS             90..461
                     /locus_tag="AC16BC_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241018.1"
                     /translation="MGMDFQVEQRPDYLLITVNEAFDLESAKEIARRSLDLCEVRKQS
                     KALGDCRNATGPLSTIDRYEYATYVAELHARYALEHGAALRVAYVGNEPLIDPNRFGE
                     TVAMNRGALIKVATDMNEALA"
     gene            570..992
                     /locus_tag="AC16BC_05050"
     CDS             570..992
                     /locus_tag="AC16BC_05050"
                     /inference="COORDINATES: protein motif:HMM:NF017482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HIR3241019.1"
                     /translation="MASKPRLFRYQLPGNWMVLAGATDEDNDYLSMELAEPADWWFHA
                     EGVPGSHVILRAKPDEEPGRETIEQAAAVAAYHSKARGAPAVRVHCARARNVTKPRGA
                     KPGTVQVAKGRVLKVRPDVSRAERLAGSGEPPSRSEDF"
     gene            1175..>1742
                     /locus_tag="AC16BC_05055"
     CDS             1175..>1742
                     /locus_tag="AC16BC_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241020.1"
                     /translation="MAMGVLIRHARAVEYGRPWSTVLICLSALFLFSCTWDIKNSRNT
                     LSNGIRSGQASEVPALSPVVQKQAKKLLNRIESAGMGVNLAEVRDRDAEELAGLIDEV
                     DDHLRRTVDDVPALILSARLHRVLLLLEPAIFGNAASGSSEHFDWSVAALDRAAAMAP
                     DNAETHYWKARVLGLKGPVVRDGALSFES"
BASE COUNT          303 a          530 c          592 g          317 t
ORIGIN      
        1 tgctacatac gtgccgggca ccacgcaccg gtggatctgg acacggtcgc ccgcgtcgcg
       61 tgtccggagc gggccaacag ggaggcgcca tgggaatgga ttttcaggtc gaacagaggc
      121 ccgattatct gctcatcacg gtgaacgagg cctttgacct cgagtccgcc aaagaaatcg
      181 cccgccgcag tttggatctc tgcgaggtcc gcaaacagtc gaaggccctg ggggactgcc
      241 gcaacgcgac cggccccttg tccacgatcg atcggtacga gtatgcgacg tacgtggctg
      301 aactccatgc ccggtacgcg ctcgagcatg gagcggcgct gcgggtggcg tatgtgggga
      361 acgagccctt gatcgacccc aaccggttcg gcgaaaccgt ggcgatgaac cgcggcgcgc
      421 tgatcaaggt ggcgaccgat atgaatgaag cgcttgcctg actgggcttt ggtccgacac
      481 acggttgacg ggacgggtgg gcaggttccg tccggtgtaa atcactcggc cccggccgac
      541 gcatcactcg ccccaatgct tgatcgctga tggcgtctaa accgcgactg ttccgttacc
      601 aactgcccgg caactggatg gtgctggccg gggccacgga cgaggacaac gactatctga
      661 gcatggagct ggccgaaccc gcggactggt ggttccacgc cgaaggcgtg ccgggcagcc
      721 acgtcatcct gcgcgccaag ccggacgagg aacccggccg cgaaaccatc gagcaggccg
      781 cggccgtggc cgcgtatcac agcaaggccc gcggcgcgcc tgcggtccgc gtgcactgcg
      841 cgcgcgcccg aaacgtcacc aaaccgcgcg gcgccaagcc gggaaccgtc caggtcgcca
      901 aggggcgcgt gctcaaggtg cggccggacg tgagccgcgc ggagcgtctg gccggatcag
      961 gcgagccgcc gtcgcgttcc gaggacttct aagtttttcg ccggcgtctc cgccgcaggc
     1021 tggccggcac ttccatgtag ggcgattgcc cttttctagg ccttttgccc tgtttccgta
     1081 ggccttgacg gaatccggat cgctatctag tcttgcccgc taattgcgaa ggggcggggc
     1141 gtcccgcctc gggtcttgtg aagggagggc acagatggcg atgggggtgt tgattcgtca
     1201 cgcgcgggcg gtcgagtatg gccgaccatg gtccacagtt ctcatctgtt tatcggcttt
     1261 attcttattt agctgtacct gggatatcaa gaactcacga aatactttaa gtaatggcat
     1321 ccgctctggg caggcgtcag aggtcccggc gctttctccc gttgtccaaa aacaggcgaa
     1381 gaagctgctg aaccggatcg agtcagccgg aatgggcgtg aatttggctg aggtccgtga
     1441 tcgcgacgcc gaggagctgg ccggtctgat cgacgaggtc gatgaccacc tcaggcggac
     1501 cgtggacgac gtgcccgcat tgattctgtc cgcgcggctg catcgcgtcc tgttgctgct
     1561 ggagccggcc atcttcggca acgcggcatc gggttcgtcc gagcactttg attggagcgt
     1621 ggcggcgctc gaccgggcgg cggccatggc gccggacaac gcggagacgc actactggaa
     1681 ggcgcgcgtg ttggggctca agggaccggt ggtccgcgac ggcgcgttgt cattcgagag
     1741 cc
//
LOCUS       DBNGTT010000227         6568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2347843, whole genome shotgun sequence.
ACCESSION   DBNGTT010000227 DBNGTT010000000
VERSION     DBNGTT010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2347843"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1874
                     /locus_tag="AC16BC_05060"
     CDS             141..1874
                     /locus_tag="AC16BC_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3241021.1"
                     /translation="MRTFLSKKRLGDILIETGAVSADVVTDAAERAMREQRWLGAILV
                     EQGMIDEEQLAKALAEQWRLPYYRLDGFYVNQEFFKTIPVELMYRYPFVPYEDRDGVL
                     TIVLADPTNLPLLDELELVLRRELRFGIGSRSGILEALKRSEGSSQVLRRIEADFRPV
                     LIKEDDRGEEVLSVEKISKDTSPVVRLVDTMILNALQKRASDIHIEVGDRGVVVKYRV
                     DGVLYSAMEPLDLKFHAPLISRIKIMSELDIAEKRVPQDGRFKLRLEQKKVDFRVSIL
                     PGIFGEAVVIRILDKEYITAGVNQLRLDRLGFNPEDLRRFRRSIIEPYGMVLVTGPTG
                     SGKTTTLYAAIEEINNQDDKIITIEDPVEYQLRNVVQIPVNEKKGLTFAKGLRSILRH
                     DPDKIMVGEIRDAETAQIAIQSALTGHLVFTTVHANNAFDVIGRFVNMGIEPYNFVSS
                     LNCILAQRLVRGLCAHCREVAPVTREACETSGLNFEQYRDAQFFQGRGCPECHGTGFK
                     GRLAITEFLDLSDQIREMILDRRPSSEIRKAAIAEGLTSLRQAGVEKVVRGETTLREI
                     NRVTFIESSGA"
     gene            1910..2809
                     /locus_tag="AC16BC_05065"
     CDS             1910..2809
                     /locus_tag="AC16BC_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HIR3241022.1"
                     /translation="MAPDALTLVRVRERGRRRVVVEHRTEPLPEGVVVVSPVERNIVD
                     EAAFDRALSAVAGRRGGPVSVALPDPVARVGLFDVSEVPTRPQEFDRLVRWHLEKSYA
                     VELGAARLTSQRFARPDGEPGARILGSAVAQTLLAQYESALARVGFEAEVIDLGAFHR
                     FNLFRERVTAAAGPGQHFIVLIVTVAALSLLIFDGGRPAYIRIKGTRRPMIGADAAAR
                     IVDEVDLSLNAYGKEKDLSRVTHLFVCAADPADTLPDALAERFHLTVELITSRNAGIE
                     FLAAMPGDSWMRLAGALGAAVGR"
     gene            2825..3400
                     /locus_tag="AC16BC_05070"
     CDS             2825..3400
                     /locus_tag="AC16BC_05070"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR3241023.1"
                     /translation="MQPQEPEVFRTNFGSQEYARSRALAAALYVAALGGFVLAAGMGW
                     WVSESQREAAGLEDNMVRVRQQASRLGEELRRAGFPPDDPAALDRLVKQVGGLNQIIE
                     SKALSWTALMNDLETVVPRNVSVSSIRPDLKTRKLTIDGVALTLQDVTALMTALQASG
                     RFTDVFLQQQKATEENRTEFTIECVYRGAHA"
     gene            3397..3915
                     /gene="pilO"
                     /locus_tag="AC16BC_05075"
     CDS             3397..3915
                     /gene="pilO"
                     /locus_tag="AC16BC_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HIR3241024.1"
                     /translation="MTRMRLRRLAVPIALATMAAVTDVGMYAAVVYPSRQTVERNDAA
                     WKAERERLARYKNYQQAYTQVTSLTARATARDDLPTVVTMLASLAKKRGLKIPEVKYQ
                     PERLDLKDFQKVGLTFSIAGPYADIRRFLNDLERSSPFLAIDGLTLTRGREKESAEIE
                     VQVKVAAYLRTA"
     gene            3920..4483
                     /locus_tag="AC16BC_05080"
     CDS             3920..4483
                     /locus_tag="AC16BC_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241025.1"
                     /translation="MDRKVKWLIGLVALWAVLLGYRIATHEPQRTAPLKYTQGATASP
                     ALGRDRDPQLHLKADLLDKPGETVGSPRNVFAPIYVYVPPPPSPPPPPAPPPPPPAPP
                     PPPPPTEEELAAQRARAELAQYKYLVYLSRGGRDQAFVSRGGETFTAGRGDPLVGGVV
                     LKEITPAYIILLHPPTRIELTVALSGS"
     gene            4682..6376
                     /locus_tag="AC16BC_05085"
     CDS             4682..6376
                     /locus_tag="AC16BC_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3241026.1"
                     /translation="MAQPQQKRKKLGEMLLAEGLLTAEQLETALAEQRRHGGRIGALL
                     KSLGFVTEEGIIKTLGRQTGIPHQVLATVIIDPDVVKRVPELLARRHQAIPLYKKSTT
                     LTLAMVDPLNVVAIDDIARATGLDIQPVVATEQDVMRAIERQYGMTATIQEAVKDLDP
                     VEEDSSAIAAERLAEDTPVVKFVTTIITQAVHDGASDVHIEPDADVLRVRHRVDGILQ
                     EVTTAPKGLQGGVLSRVKILADLDISERRIPQDGRIQMKVGERDIDLRVSTLPTIYGE
                     KVVLRILDKRQGIVKLPDLGFSPGPFEVVQRLVARPYGLFLVTGPTGSGKTTTLCAAL
                     NTINTMARNIVTIEDPVEYQLKIINQVQVNPKAGITFANGLRSILRQDPDVIMVGEIR
                     DRETATIAIQAALTGHLVFSTLHTNDAPSAIARLVDIGVEPYLIASSLIGVVAQRLVR
                     KVCPSCRKPATVPDGVFEGIAPEGGTLFPAKGAGCSGCRNTGYNGRTGVFELLTVNDA
                     LRDLIVTRSSASAMSTAAVQTGYVQLRQAGLAKAASGITTLEEVYRVTQDADADVG"
BASE COUNT         1246 a         1996 c         2152 g         1174 t
ORIGIN      
        1 cacgaagagg aattggaccg cacgttgacc cggatcacga cctgggtcga gcccgtgtta
       61 ttgctgacca tgggggtgct ggtggctgcg gttgtggtca cgatgtatct gccgattttc
      121 aatttggccg ggacaatcaa gtgaggacgt ttctctccaa aaagcggttg ggcgatatcc
      181 tgatcgaaac cggtgcggtc agcgccgacg tggtgaccga cgccgcggaa cgagccatgc
      241 gtgagcagcg ctggttgggc gcgatcctcg tcgagcaagg aatgatcgac gaggaacaac
      301 tggccaaggc gttggccgag cagtggcgcc tcccgtacta ccggctggac gggttctacg
      361 tgaatcaaga gtttttcaag accattcccg tggagttgat gtaccgctat cccttcgtgc
      421 cgtacgaaga ccgggacggc gtgctcacga tcgtgctggc cgatcccacg aacctgccgt
      481 tgttggacga actcgagttg gtgttgcgcc gggaactccg gtttggtatc ggcagccgga
      541 gcgggattct ggaggcgctc aaacgcagcg aggggtcgtc gcaggtcctc aggcggatcg
      601 aggcagattt ccgcccggtg ttgatcaaag aagacgaccg cggggaggaa gtcctctcgg
      661 tggagaagat ttccaaggac accagtccgg tcgtccgtct ggtggacacc atgatcctca
      721 acgcgctgca gaagcgcgcg agcgacatcc acatcgaagt cggggatcgg ggcgtggtgg
      781 tgaagtaccg cgtggacggg gtgctgtact ccgcgatgga accgttggac ctcaagttcc
      841 atgcgccgct gatttcgcgc atcaagatca tgtcggaact cgacatcgcg gagaagcggg
      901 ttccccagga cggccggttt aaactccgct tggaacagaa aaaagtcgat ttccgcgtgt
      961 cgatcctccc cgggattttc ggcgaagccg tggtcatccg gatcctggac aaggagtaca
     1021 tcaccgcggg cgtcaaccaa ttgcgcttgg accgcttggg ctttaacccc gaggacctgc
     1081 gccgcttccg ccgttcgatc atcgagccgt acgggatggt cctcgtcacc ggccccaccg
     1141 gcagcggcaa gaccacgacg ctgtacgcgg cgatcgagga gatcaacaat caggacgaca
     1201 agatcatcac gatcgaagac cccgtcgagt accagttgcg gaacgtggtg cagattccgg
     1261 tcaacgagaa aaagggcctg acgttcgcca aggggttgcg ctcgatcctt cggcacgacc
     1321 cggacaagat catggtcgga gagatccgcg acgccgaaac cgcgcagatc gcgatccagt
     1381 cggcgctgac cggccacctg gtgtttacga cggtgcacgc caacaacgcg ttcgacgtga
     1441 tcggccggtt cgtgaacatg gggatcgaac cgtacaactt cgtgtcgtcg ctgaattgca
     1501 ttctcgcgca acgactggtg cgcgggctct gcgcgcactg tcgggaagtc gcgccggtca
     1561 cgcgtgaggc ctgcgaaaca tccgggttga acttcgagca gtaccgggac gcccagttct
     1621 tccagggccg cggctgtccc gagtgtcatg gcacgggatt caaggggcgg ctggcgatca
     1681 cggagtttct cgacttgtcc gatcaaattc gtgagatgat cctggaccgc cgtccgtcgt
     1741 ccgagatacg gaaagccgcc attgccgagg gcctcacgtc gcttcgtcag gccggcgtgg
     1801 aaaaggtcgt gcggggcgaa accacgttgc gcgagatcaa ccgggtcacc ttcatcgagt
     1861 cctccggagc gtgatgggtt ggcgacacgg ggtccggacc ggactcgact tggcaccgga
     1921 cgcactcact ctcgtacgcg tgcgcgagcg cggccggcgg cgcgtcgtgg tggaacaccg
     1981 gaccgaaccg cttccggagg gggtggtggt ggtgtcgccg gtcgagcgca acatcgtcga
     2041 cgaggcggcg ttcgaccggg cgctgagcgc ggttgcggga cgccgcggag ggccggtgtc
     2101 ggtggcgctc cccgatccgg tggctcgggt ggggttgttc gatgtatcgg aagtgcccac
     2161 gcgcccgcag gaattcgacc ggctggtgcg gtggcatctg gagaaatcgt acgcggtgga
     2221 gctgggggcg gcgcgactga cgtcccaacg cttcgcgcgg ccggatggag agcccggtgc
     2281 ccggatcctg ggttctgcag tggcgcagac cctgctcgcc cagtatgaaa gcgcgctggc
     2341 ccgtgtcgga tttgaagccg aggtcatcga cctcggagcg ttccatcgct tcaacctctt
     2401 ccgggaacgc gtcacggccg cggccggccc gggtcagcat tttatcgttc tcatcgtcac
     2461 cgtggcggca ctgtccttgc tgatcttcga cggcggccga cccgcgtaca tccgtatcaa
     2521 gggcacccgt cggccgatga tcggcgcgga tgccgcggcg cgcatcgtgg acgaggtcga
     2581 cttgtcgctc aacgcctacg ggaaagagaa agacctctcc cgcgtcaccc acctcttcgt
     2641 gtgcgccgca gatcccgccg atacgttgcc cgacgcgttg gcggagcggt ttcatctgac
     2701 cgtcgaactc atcacgtccc ggaacgccgg gatcgagttc ctcgccgcca tgcccggaga
     2761 ttcgtggatg cggctggcgg gcgccctcgg cgccgccgtg ggccgctaag ccgcgatggc
     2821 ggcggtgcaa ccacaagaac cggaagtctt ccgaactaac ttcggatccc aagagtacgc
     2881 gcgctcccgc gcgctcgccg cggcgctgta cgtcgccgcg ctcgggggat tcgtcttggc
     2941 cgccgggatg gggtggtggg tgtctgaaag ccagcgcgag gcggcggggc tcgaggacaa
     3001 catggtgagg gttcgacaac aagcgtcgcg cctaggcgag gagttgcgcc gtgccggatt
     3061 ccctccggac gacccggccg cgctggatcg actcgtcaag caggtcggcg gactgaacca
     3121 aatcatcgag agcaaagctc tgtcatggac ggccttgatg aacgatctgg aaaccgtggt
     3181 gccgcgcaac gtgtcggtca gcagtatccg tcccgacctc aagaccagga agctcacgat
     3241 tgacggggtg gcgttgacgc tccaggacgt caccgcgctg atgaccgccc tgcaggcgag
     3301 cggccggttt accgacgtgt tcctgcaaca gcagaaggcc accgaggaaa atcgcaccga
     3361 gtttacgatc gagtgcgtgt accgtggagc gcacgcatga cgcggatgcg tctccgacgg
     3421 ctcgcggtgc ccattgccct ggcaacaatg gccgcggtca ccgatgtcgg gatgtatgcg
     3481 gccgtggtgt atccgagccg ccagacggtg gagcggaacg acgccgcctg gaaggctgag
     3541 cgtgaacgct tggcgcggta caagaattat cagcaggcct acacccaggt gacgtcgctg
     3601 acggcgcgcg cgaccgcccg agacgatctc ccgaccgtgg tcacgatgct ggcgtcgctg
     3661 gccaagaagc gcggcctgaa gattccggag gtgaagtacc agcccgagcg cctggatctc
     3721 aaggacttcc aaaaggtcgg tttgacgttt tcgatcgcgg gaccctacgc cgatatccgg
     3781 cggttcttga acgatcttga gcggagcagc ccgtttctcg cgatcgacgg gctgacgttg
     3841 actcgaggac gcgagaagga atccgcggag atcgaggtcc aggtgaaggt cgcggcctat
     3901 cttcggaccg catagcgcca tggaccgtaa agtgaaatgg ctgatcggcc tggtcgccct
     3961 gtgggcggtg ctgttgggct accggatcgc gacgcatgag ccgcagcgaa ccgcgccgtt
     4021 aaaatatacc cagggcgcca ccgcctcgcc cgccttgggg agagaccgcg acccgcaact
     4081 gcacctgaag gccgatctgc tggataaacc cggggagacg gtgggatcgc cgcgaaacgt
     4141 gttcgcgccg atctacgtct atgtgccgcc gcccccttct cccccgccgc ctcctgctcc
     4201 gcccccccct ccacccgcgc ccccgccgcc accgccgccg acggaggagg agttggccgc
     4261 ccaacgggcg cgcgccgaat tggcgcagta taagtatctc gtgtatttga gtcgcggcgg
     4321 ccgcgaccag gcattcgtgt cacgcggcgg cgaaacattc accgccggcc gcggagatcc
     4381 gttggtcggg ggcgtcgtgc tcaaggaaat cacgcccgcc tacatcatcc tgttgcaccc
     4441 tccgactcgg atcgagctca ccgtcgcgtt gagcgggtcc tagtttgtag ctcagcacgc
     4501 ccgccacgcg gggtctagcg caccctcgcg acgaacgtcc acgagagacg cgcgcgaatc
     4561 cggcggcgcg ccgccgattt cgactcgcgt caccatcgtt tcaggcccat tctgccttgc
     4621 ggaattgaag gcctctgcta catttgccat gccgttgcct ggatcagccg acatcccact
     4681 tatggcgcag ccccaacaga aacgaaaaaa actcggcgag atgttgttgg ccgagggctt
     4741 actgactgca gaacaacttg aaaccgcgtt ggcggagcaa cgccgtcacg gcggccgcat
     4801 tggcgcgctc ctcaaaagcc tgggcttcgt caccgaagag ggcatcatca agacgttggg
     4861 gcgccagacc gggattccac atcaggtgct cgcaaccgtc attatcgatc cggacgtggt
     4921 gaaacgcgtg ccggaactgt tggcgcgtcg gcatcaagcg attccgctct ataaaaaaag
     4981 caccacgttg accttggcca tggtggaccc gctcaacgtg gtggcgatcg acgatatcgc
     5041 ccgcgccacc gggctggata tccagccggt tgtggcgacc gagcaggacg tcatgcgcgc
     5101 catcgagcgc caatacggga tgaccgcgac gattcaggag gccgtcaagg acctcgatcc
     5161 ggtggaggag gattcatccg ccattgcggc cgaacggctg gccgaggaca cgccggtcgt
     5221 gaagttcgtc accaccatca tcacccaggc ggtgcacgac ggggcgagtg acgtccacat
     5281 cgaacccgac gcggacgtgt tgcgtgttcg ccaccgcgtg gacggcattc tgcaagaggt
     5341 gaccaccgcg cccaagggat tgcagggcgg cgtgctctcc cgggtcaaga tccttgcgga
     5401 tctggatatc tcggagcgcc ggattccgca ggacggtcgg atccaaatga aagtgggtga
     5461 gcgcgacatc gatctgcggg tgtccaccct ccccacgatc tacggcgaga aagtggtgct
     5521 ccgtatcctg gacaagcgcc aggggatcgt gaagctgccg gatctgggat tttccccggg
     5581 gccgttcgag gtggtgcaac gattggtggc gaggccctat gggctgtttc tggtcaccgg
     5641 cccaaccggg agcgggaaaa cgaccacgct ctgcgcggcg ttgaacacga tcaacaccat
     5701 ggcgcgcaac atcgtgacga tcgaggaccc cgtcgaatac caactgaaaa tcatcaatca
     5761 ggtgcaagtc aatcccaagg ccgggatcac attcgccaac ggcttgcggt ccatcttgcg
     5821 tcaggaccct gacgtcatca tggtcgggga aattcgagat cgtgagaccg ccacgatcgc
     5881 gatccaggcg gcgctgacgg gccatttggt cttctcaacc cttcatacca acgatgcccc
     5941 gagcgcgatc gcccgcttgg tggacatcgg ggtggagccg tacctcatcg cgtcgtcgct
     6001 gatcggggtc gtggcccagc ggctggttcg aaaagtctgc ccgtcgtgtc ggaagcccgc
     6061 cacggttccg gatggcgtgt ttgagggtat tgcccctgaa ggggggacat tgttcccggc
     6121 aaagggggcg ggatgttcgg ggtgccgcaa cacgggttat aacggccgaa ccggggtctt
     6181 tgaactcctg accgtcaacg acgcgttgcg cgatctgatc gtgacccgat catcggcgtc
     6241 cgcgatgagt acggctgccg tccaaaccgg atatgtccag ttgcgacaag ccggtctagc
     6301 aaaggccgcg agcggcatca cgacgctcga agaagtttat cgcgtcaccc aagacgcgga
     6361 tgccgatgtg gggtgacgat ttctggtctc actgtgaaat gtgttccaca gccacgtggg
     6421 tcatgtaacc aaaatttgaa gagaaccgtt tcttcagaca acacaggaag atccgatggc
     6481 gcatcgattg cactgaggcc cacagatggc gcggtatcgc tacaaagccc gtgataaatc
     6541 agggagcgtg atgaccgggg tcatcgag
//
LOCUS       DBNGTT010000228         3609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_49078, whole genome shotgun sequence.
ACCESSION   DBNGTT010000228 DBNGTT010000000
VERSION     DBNGTT010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_49078"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="AC16BC_05090"
     CDS             <1..1181
                     /locus_tag="AC16BC_05090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR3241027.1"
                     /translation="DAHTHVLAYVFNVIAADHADTDRLRKLPTPMASRNLANEIDQPA
                     VDALLAAVERGYPVVQRYYRLKRRLLGVERLYDYDRYAPLATKDRAIRWDDARKTVLD
                     AFGAFSPDMAAIASRFFEDRWIDAAIRPGKRGGAFSHGAVPSAHPYVLMNYAGTSRDV
                     MTLAHELGHGVHQYLSRPKGCLQADTPLTMAETASVFGEMLVFRALKDAERDPRRALA
                     LVCGKIEESCSTIFRQVVLTRFEQSLHEARRREGELPVDRINTLWRDANRAMFGDSVT
                     LTEDYSRWWSYIPHFIHSPFYCYAYAFGALLVFALYQRYVEAGPGFAPKYLALLSAGG
                     SDAPERLLAPLGIDLSAPGFWDGGIAVLASWVDEAEELAGTVAGSRQGASGRRRASTR
                     "
     gene            complement(1170..1778)
                     /locus_tag="AC16BC_05095"
     CDS             complement(1170..1778)
                     /locus_tag="AC16BC_05095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3241028.1"
                     /translation="MDTRTLDVYRAGIDAYLAQWGLRRYRVPPLLRELISTLPRGAWV
                     LDLGCGPAQDARYLAQKGYRSVGLDAVQEFLAWARSVSAATPLIRADMRHLPFGARAF
                     DAVWAAASLIHLPKRDVRVALAALHTIVAPGGRLAATFVHGTASGVLARGWLPGRYVS
                     RWTNAELARALERSGWNINSLKTVTNLERKGRWLNLTARSSG"
     gene            complement(1874..2590)
                     /locus_tag="AC16BC_05100"
     CDS             complement(1874..2590)
                     /locus_tag="AC16BC_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241029.1"
                     /translation="MTNHSTAWAQRLRLTTITAVLVGGMSVVAQAAPLGVPAATTGAG
                     KTALGAEVNVLMDRDLTGGNGEVDGSQIYAKGSLGIDDRLDLDFRLGFGDFSLSPGGF
                     DTDIGPAFGVGMRVTWATIPNANVKVGSVFQTTRIRAEESTTGLRVGWAEYDASLGVS
                     LDMSGPRDAKTRQTQFGLVPYGGLAWSGLDLDVLGASEDDAFGLFLGLNARAQGNFNF
                     GVELRLVDQTALSVSAGTTF"
     gene            2805..3584
                     /locus_tag="AC16BC_05105"
     CDS             2805..3584
                     /locus_tag="AC16BC_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241030.1"
                     /translation="MPAVRLAAFALAALVLAACGGGAATEPPPPTPSIAIIPPSVTLP
                     VGGGVTFTAVVDGATDPSVTWSSTGGTITQDGVFTATTASSEPYIVTATLTADTTVSA
                     TATVTVNPAPVVSLSISPSQPTVVVGRTQLFTARVVGGSTSEVTWSVEPAGGGTITSD
                     GLYTAPAQTGTFTVAAETKAAPITRKTVFVTVVDQPVPAVEIDDLSADPAKPMVNGET
                     RLFQARLTNASLVEVFWSVTPVDPPATANSLPMPSVTPTGS"
BASE COUNT          619 a         1202 c         1162 g          626 t
ORIGIN      
        1 tcgacgcgca cacgcacgtg ttggcctacg tcttcaacgt cattgccgcg gaccacgcgg
       61 acacggatcg gctgcgcaag ctgcccacgc ccatggcctc gcgcaacctg gccaatgaaa
      121 tcgaccaacc cgctgtggac gcgctcctcg ccgcggtgga gcgcggatat cccgtggtgc
      181 aacgctacta ccggttgaag cggcggctgc tgggcgtgga gcgtctgtat gattatgacc
      241 gctacgcgcc gctggcgacc aaggaccgcg cgatccgatg ggacgatgcg cgcaagacgg
      301 tgctcgacgc gttcggcgcg ttttcgccgg acatggccgc gatcgcgtcg cgatttttcg
      361 aggaccggtg gatcgacgcg gcgatccgtc ccggcaagcg gggcggcgca ttctcccacg
      421 gcgcggtgcc gtcggcgcac ccctacgtgc tcatgaacta cgccggaacc tcgcgcgacg
      481 tgatgacgct cgcccacgag ctcggtcacg gcgtgcatca gtacctgtcg cggccgaagg
      541 gctgcctgca ggccgacacg ccgctgacca tggcggagac cgcgtcggtg ttcggcgaga
      601 tgctggtctt tcgcgcgctc aaggacgcgg aacgcgatcc gcgccgggcc ctcgccctgg
      661 tgtgtgggaa aatcgaggag tcctgctcca ccatcttccg ccaggtcgtg ctcacgcgat
      721 tcgagcagtc gttgcacgag gcgcgtcgtc gcgaaggcga actcccggtc gaccggatca
      781 acacgttgtg gcgcgacgcc aatcgcgcga tgttcgggga cagcgtgacc ttgaccgagg
      841 actacagccg ctggtggagt tacatccccc actttatcca ctcgccgttt tattgctatg
      901 cgtatgcctt cggcgccttg ctggtcttcg ccctgtatca gcggtacgtg gaggcgggac
      961 cggggttcgc gcccaaatat ctggcgctgt tgtccgccgg cgggtcggac gcgcccgagc
     1021 ggttgttggc gccgctgggc atcgacctct ccgccccagg gttctgggac ggggggatcg
     1081 ccgtgctggc gtcgtgggtg gatgaagccg aggagttggc gggaacagtg gcgggttcga
     1141 ggcagggggc gtccggtcga cgccgcgcgt caacccgatg aacgcgccgt caggttgagc
     1201 caccgcccct tgcgttccag attcgtcacg gttttgagcg agttgatatt ccagcctgat
     1261 cgctccaggg cccgagcgag ttctgcgttg gtccaccgcg agacatagcg gccaggcaac
     1321 cagccgcgcg ccagcacgcc cgacgcggtc ccgtgaacga acgtggcggc gagacggccg
     1381 cccggagcca cgatcgtgtg taatgccgcg agcgcgacgc gcacgtcgcg tttcggcaaa
     1441 tggatcagcg acgccgcggc ccacacggcg tcgaacgcgc gggccccgaa cggaagatgc
     1501 cgcatgtccg cgcggatcag cggtgtcgcc gcgctcacgg atcgagccca cgcgagaaac
     1561 tcttggaccg cgtccagccc cactgaccgg tagcccttct gcgcgagata ccgcgcgtcc
     1621 tgcgcaggtc cgcagccaag gtccagcacc cacgcaccac gcggcaatgt gctgatgagt
     1681 tcgcgtagca acgggggcac gcggtagcgg cgtaggcccc actgggcgag atacgcgtcg
     1741 atgccggcac ggtacacgtc gagggtcctt gtatccatat cacggcgcat ccggtcgcgt
     1801 ttccaatcgt caaccttgcc aacaaaaaag ccggtggagc agggctccac cggctttttt
     1861 tcaccgcatg acgctaaaac gtggttcccg cgctcacact gagggcggtt tgatcgacca
     1921 accgcagttc cactccgaaa ttgaaattgc cttgcgctct agcgttgaga ccgaggaaca
     1981 agccgaacgc gtcatcctct gatgccccaa ggacatccaa atcgagaccg gaccaggcaa
     2041 ggccgccgta cggaaccagc ccgaactggg tctgtcgggt cttggcgtca cgggggccgg
     2101 acatgtcgag cgagacaccg agggacgcgt cgtactcagc ccatcctacc ctgaggccgg
     2161 tggttgattc ttccgcgcgg atccgtgtgg tttgaaacac gctcccgacc ttcacgttcg
     2221 cattgggaat cgttgcccat gtcacgcgca tcccaacgcc gaacgcgggg ccgatatccg
     2281 tgtcgaatcc tcccgggctg aggctaaaat ctccgaagcc gaggcgaaag tcaaggtcaa
     2341 gccggtcgtc aatcccgagc gaccctttgg catagatctg tgacccatcg acctcaccgt
     2401 tgccgccggt aaggtctcta tccatcaaga cattgacctc tgccccgagc gcggttttcc
     2461 ccgcgccggt agtggcggcg ggcacgccga gtggggcggc ctgtgcgacg acgctcatgc
     2521 cgccgacaag gaccgcggtg attgtggtca agcgcaaccg ttgcgcccat gccgtgctgt
     2581 gattggtcat gactcctcct gggtgaagat gagcgacaaa cctgtcgcgg tcgtcccgac
     2641 cgcgcgacgc ttataacaga attaggacgc ccaaaacaag acgaaaatcc ggaaccgaga
     2701 gccgggcggc ggaacgtgtc acgggggaat tttcacgccg cgctcgctgt ttctgtgtcg
     2761 agccccatcg ccttgtgctc atgaccgaca ttcggatacc cttcgtgcct gccgtgcggt
     2821 tggcggcatt cgccttggcc gcgctcgtgc tcgcggcctg cggcggggga gccgcgaccg
     2881 agccgccgcc tccgactcct tcgattgcca tcattccgcc atccgtgacg cttccggtcg
     2941 gcggcggagt gacgtttacc gcggtcgtgg acggcgcgac cgacccgtcg gtcacctggt
     3001 catccaccgg cggaaccatt acacaggacg gagtattcac ggccacgacc gccagctccg
     3061 agccgtacat cgttacggcc acgctcacgg ccgacacgac cgtttccgcg accgcgacgg
     3121 tgacggtgaa tcccgcgccc gtggtctcgt tgtccatcag tccgtcccag cccaccgtgg
     3181 tggtcgggag gacgcaattg tttaccgcgc gcgtggtggg gggctccacg tcggaagtca
     3241 cctggagcgt tgagcccgcc ggcgggggga ccatcacttc cgacggcctc tataccgcgc
     3301 cggcccaaac cgggaccttc accgtggcgg ccgagaccaa ggccgcgccg atcaccagga
     3361 aaaccgtgtt cgtgaccgtg gtcgatcaac cggttcccgc cgtggagatc gatgacttgt
     3421 ccgccgatcc cgccaagccg atggtcaacg gggaaacgcg cctgtttcag gcgcgcctga
     3481 ccaatgcgtc cctggtcgag gtgttctgga gcgtaacgcc ggttgatccg ccggcgaccg
     3541 caaacagtct gccgatgccg agcgtgacgc cgaccggaag ctagacgcag ttcaccgcca
     3601 gcgatcgtc
//
LOCUS       DBNGTT010000229         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2093856, whole genome shotgun sequence.
ACCESSION   DBNGTT010000229 DBNGTT010000000
VERSION     DBNGTT010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2093856"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1462
                     /gene="xseA"
                     /locus_tag="AC16BC_05110"
     CDS             59..1462
                     /gene="xseA"
                     /locus_tag="AC16BC_05110"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR3241031.1"
                     /translation="MNLFEFSAQSDAPTPPPRQILTVSDLTGLVRRRLEGDFGDVWVE
                     GEISNLRNPGSGHYYLTLKDAQSQLRAVVFRSVSRVVKFALKDGLAVICRGHVTVYEP
                     RGEYQLVVDYVEPKGAGALQLAFEQLKERLARDGLFDAARKRALPFLPTRLGVITSPS
                     GAVIRDILHVLKRRFPTIPVLLLPVPVQGPGAAGRIADAIDEANALSERLRPDVLIVA
                     RGGGSLEDLWAFNEEIVARAIAASAIPVVSAVGHETDYTIADFVADLRAPTPSAAAEL
                     VVPRRDYLMATVATASERLAEAARSALDDRRARVSAELRALRTPERLIESALLRVDDL
                     SARLRESIARVLAGRREQRRFLSRAVLAAHPSRRIETSRLAHQRAAERLAERIRMIVS
                     DAKARVAGLAARTDALSPLAILGRGYSLTRLLPSRRIVRTAADVVPGDRLLITLGSGE
                     VTAEAREIRDDAEMSGS"
     gene            1459..2646
                     /locus_tag="AC16BC_05115"
     CDS             1459..2646
                     /locus_tag="AC16BC_05115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR3241032.1"
                     /translation="MTAPLALTVKPPALGRLEAGHPWIYANELARPPKTAPPGSVVDA
                     RTAQGKWIGRGYYNPKSVIAVRLLSREAVEIDAAFIACAIGRAQSLRDAVVPGEEAYR
                     VVFGEADGLPGLIVDRYGSVLVVQILTAGMDRLTDAIVAALVERFQPTAIVARNDAGS
                     RKLEGLAVEKRLLYGTKPDRVVVIKNGLSFEVDVWEGQKTGLFLDQSANYRAIAPWAK
                     GARVLDTFCYAGAWGLHALHYGAASVVGLDASEKAVATAATNASRNGLSERAEYRRVD
                     VFDELRTLLAAGAQYELIVLDPPSFAKARKDKAGALRGYKEINRLGMSLLAPGGVFVT
                     CSCSHPIDAATFHNVLVEAAADAKRTFRLIERRTQGPDHPIVLGIPETAYLQCVMLQD
                     LRN"
     gene            2769..>3242
                     /locus_tag="AC16BC_05120"
     CDS             2769..>3242
                     /locus_tag="AC16BC_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3241033.1"
                     /translation="MKIIADLHIHSYLSRACSPQLRPARLYEWCQLKGITALATGDFT
                     HPAWFAELRDTLVETEAGLFTLKPDIAKPVDDVLFPSCRAPVRFVLGVEISCIYKKGP
                     RVRKVHHLVFMPSFEAAERFNASLAKIGNIRSDGRPILGLDSRNLLELVLKADSQG"
BASE COUNT          547 a         1090 c         1039 g          566 t
ORIGIN      
        1 gagcccgtcg aaggacgctc ccgcgggttt ccaagcacct tgaatcgtgg cctaaccgat
       61 gaacctcttc gagttctccg cccagtccga cgcgcccact ccaccaccgc gacagattct
      121 cacggtctct gatctgaccg gactcgtgcg tcgccgcctc gaaggcgact tcggcgacgt
      181 gtgggtcgag ggggagattt ccaacctccg caaccccggg tccggccact attacctgac
      241 gctcaaagac gcgcagagcc aactccgcgc cgtggtgttc cggtccgtgt cgcgggtcgt
      301 gaagttcgcc ttgaaggacg gcctcgccgt gatctgtcgc ggccacgtca cggtgtacga
      361 accgcggggc gagtaccaac tcgtggtgga ctacgtggag cccaagggcg cgggcgccct
      421 gcaactcgcg ttcgaacagc tcaaagagcg cctcgcgcga gacggcctgt tcgacgcggc
      481 gcgcaaacgc gccctcccgt ttcttccgac caggctcggc gtgatcacgt ccccgtccgg
      541 cgcggtgatc cgggacatcc tgcacgtcct caaacgccgt tttcccacga tcccggtgtt
      601 gctgctcccc gtgcccgtgc agggtcccgg cgcggccggc cggatcgcgg acgcgatcga
      661 cgaggccaac gcgttgtccg aacggcttcg accggacgtg ctcatcgtgg cgcgcggcgg
      721 gggctcgctg gaagacctct gggcattcaa cgaggaaatc gtggcgcgcg cgatcgcggc
      781 gtccgccatc cccgtggtct cggccgtggg ccacgaaacg gattacacca tcgcggactt
      841 cgtcgccgat ttgcgcgcgc ccacgccttc ggccgcggct gaactggtcg tcccgcgacg
      901 cgactacctg atggccaccg tggcgaccgc aagcgaacgg ctcgcggagg cggcccgctc
      961 cgcgttggac gaccggcgcg cgcgggtatc cgcggagctt cgcgcgctgc gaactccgga
     1021 acggttgatc gaaagcgcct tgctgcgcgt ggatgacttg tccgcgcgat tgcgcgagtc
     1081 catcgcacga gtgctcgctg gccgtcgcga acagcgccga tttctgtccc gcgccgtcct
     1141 ggccgcgcac ccgagccgac ggattgagac ctcgcgcctc gcgcaccaac gagcggccga
     1201 acgattggcc gagcgcatcc gcatgatcgt gtcggacgcg aaagcccgcg tggccggcct
     1261 cgccgcgcgc acggatgcct tgagcccgct cgcgattctc ggccggggct acagcctgac
     1321 tcgactgctc ccctcacgcc ggatcgtgcg aaccgccgcg gacgtggtcc ccggcgaccg
     1381 cctgttgatt acgctcggca gtggtgaagt cacggccgag gcccgcgaga tacgcgacga
     1441 cgcggaaatg tctggatcgt gaccgcgccg ctcgcgctta cggtcaaacc gcccgcgctc
     1501 ggtcgtctcg aagccgggca tccctggatt tacgcgaacg aactcgcgcg cccgccaaaa
     1561 accgcgccgc ccggctcagt ggtggatgcg cgaaccgcgc agggaaagtg gatcggccgc
     1621 gggtactaca accccaagtc cgtgatcgcg gtgcgtctgc tctcgcgcga ggccgtagag
     1681 attgatgccg ccttcatcgc gtgcgcgatc ggtcgcgctc agtccctgcg ggacgccgtg
     1741 gtgcccggcg aggaagcgta tcgcgtggta ttcggagaag ccgatggctt gcccgggttg
     1801 atcgtggatc ggtacgggtc cgtgctggtc gtacagatcc tcaccgcggg catggatcgg
     1861 ctgaccgatg ccatcgtcgc cgcgctcgtt gagcgtttcc agccgaccgc gatcgtggcg
     1921 cggaacgacg cgggctcgcg aaaactcgaa ggtctggccg tggagaagcg gctgctgtac
     1981 ggcaccaaac ccgatcgcgt ggtcgtgatc aaaaacggcc tgtcgttcga ggtcgacgtg
     2041 tgggagggtc agaagaccgg gttgtttctc gatcagtcgg ccaactaccg cgcgatcgcc
     2101 ccatgggcca agggcgcacg ggtgctcgat accttctgct acgcgggcgc gtggggcttg
     2161 cacgcgttgc actacggcgc cgcgagtgtg gtgggactcg atgcttccga gaaagccgtg
     2221 gcgacggccg cgacaaatgc gtcgcgaaat ggactgagtg agcgcgcgga gtaccggcgc
     2281 gtcgacgtgt tcgacgagtt acgcacgctt ctcgccgcag gcgcccaata cgaactgatc
     2341 gtcctcgacc cgccatcctt cgcgaaagcg cgcaaagaca aggctggtgc gttacgtggc
     2401 tacaaggaga tcaatcgtct cggcatgtcg ctgctcgcgc ccggcggcgt cttcgtcacc
     2461 tgctcgtgct cgcacccgat cgatgccgcc acgtttcaca atgtcctggt ggaggcggcc
     2521 gccgacgcca agcgcacgtt ccgcctgatc gaacgccgga cgcaagggcc ggatcatccg
     2581 atagtgctgg gcattccaga gacggcgtat ttacaatgcg tgatgctgca ggatctgaga
     2641 aattgaggca accacgccat tagaaaatcc ccccattccc ctttgtcaaa ggggaatggg
     2701 gggatttggt tttgtaccgc agatcaggac tagtcacccc tacggggtcg tgatacgata
     2761 cgccacccat gaagatcatc gccgacctcc acatccactc ctatctttcg cgggcgtgca
     2821 gcccccaact ccgacccgct cggctctacg agtggtgcca gctcaagggc atcaccgcgc
     2881 tcgccaccgg ggacttcacg cacccagcct ggttcgccga actgcgagac acgctggtcg
     2941 aaaccgaggc cggcctgttc acgctcaagc cggatatcgc caaaccggtg gatgacgtac
     3001 tgttcccgtc gtgtcgggcg ccggtgcggt ttgtgctcgg cgtggaaatc agctgtatct
     3061 ataaaaaggg cccgcgcgta cgcaaagtcc accacctggt cttcatgccg tcgttcgagg
     3121 cggccgagcg cttcaatgcc agcctcgcca agatcggcaa catccgctcc gatggccgac
     3181 cgattctggg actcgattca cgcaacctcc ttgaactggt tttgaaggcc gactcgcagg
     3241 ga
//
LOCUS       DBNGTT010000230         2672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2859248, whole genome shotgun sequence.
ACCESSION   DBNGTT010000230 DBNGTT010000000
VERSION     DBNGTT010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2859248"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..700)
                     /locus_tag="AC16BC_05125"
     CDS             complement(179..700)
                     /locus_tag="AC16BC_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241034.1"
                     /translation="MVSFPILASFLGFFSATPATSSQPPFLSTVDPGCERALPVRVVQ
                     AVSGRSGVGLVPRDSRHGAHGTCNYAIDGKDVILSVAINPLTKPEFYESYRRNCGYVE
                     PGTPIAGLGDEALACSAYGGGADRAVVVHTERRVVVIQSAKRFDPQARRVRENYFTID
                     QLTELARTVVKKL"
     gene            complement(825..1277)
                     /gene="rplI"
                     /locus_tag="AC16BC_05130"
     CDS             complement(825..1277)
                     /gene="rplI"
                     /locus_tag="AC16BC_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3241035.1"
                     /translation="MRIILREDVDHVGKMGELVTVKDGYARNFLLPRRMAAPATERNV
                     HALEHEKRVIEAKRKKERVAAEELVKRLASLPLTFAMQAGEDDKLFGSVTSKDIADAL
                     AAKEFVVDKRKIVLDKPIKALGTFAVPIKLASDVTGEVSVSVVKEATA"
     gene            1481..1900
                     /locus_tag="AC16BC_05135"
     CDS             1481..1900
                     /locus_tag="AC16BC_05135"
                     /inference="COORDINATES: protein motif:HMM:NF022591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HIR3241036.1"
                     /translation="MKRLALIVLGLSLTTPVFAQEPVAPVAAPASDKVFRAVFTTAVT
                     NREPADQVTSLKSDVQQVFFFTELSGLDGQTITHKWEHAGEVKAEVKFDVKAPRWRVW
                     SSKKFDPSWTGEWTVSVVDGAGAVLAQGKLMYEVAGQ"
     gene            complement(2011..>2672)
                     /locus_tag="AC16BC_05140"
     CDS             complement(2011..>2672)
                     /locus_tag="AC16BC_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241037.1"
                     /translation="ARIIEVQRFSGNCEALVTENRVLSWTNVIIAGSAVATLVLALVQ
                     NCQTSDLVRQAGHAFSASTLPLIKYDQYQWFAPRNLSCENPASGFNAYFRNVSGVPIF
                     VEKSDLEVFMGDRLVATPKGAEVSLKVPGELILAPGQSIADGFEDPSFPDVYKRLRGP
                     GSTPHLNFRLSVAYRSAVTGQQYCYSAKVVILDDCELPSVHHSQVGDEKLVEGACKEP
                     A"
BASE COUNT          576 a          847 c          755 g          494 t
ORIGIN      
        1 ccatgcggcg gatcgtcaat tcgcagccca acagacggcg accacctcgc cgaaggccat
       61 gtccactgca tgcaacaata cgccgccttg ccgacgactg caagtcagcg atgctgggga
      121 gggatgggga aaaggccgcc ggacaactcg ggagtcgcgc gtccgccgcg gcccaagctt
      181 aaagtttttt cacgactgtg cgcgccagct cggtcaactg gtcgatggta aaataattct
      241 cacgcacgcg gcgcgcctgg ggatcgaagc gtttggcgct ttggatcacc acgaccctgc
      301 gctcggtgtg caccaccacc gcgcgatccg ccccgccgcc gtacgccgag caggccaatg
      361 cttcgtcgcc caatccggcg atcggcgtgc ccggctcaac gtatccgcaa ttgcgccggt
      421 agctttcata gaattcgggc ttggtcagcg gattgatcgc cacggagagg atcacgtcct
      481 tcccgtcgat cgcgtaattg caggtcccgt gcgcaccgtg tcgcgaatcc cgcggcacca
      541 gccccacgcc cgaacgaccc gacacggctt ggaccacgcg aaccggcagc gcgcgctcgc
      601 acccgggatc cacggtggag agaaacggcg gttggctgga tgtggcgggc gttgcgctga
      661 agaaccccag aaacgacgcc aggatcggaa aagagaccat cgccgtgcct ccgaacgagg
      721 ggacgaagat gagtctagtc taacggagat cgcgcggctg ggcaaccgct agacggcacc
      781 ggcgatcggc aagagcccgg cgggtcggcg aatcgagcgg cgggttacgc cgtggcttct
      841 ttgacgaccg acaccgagac ttcgccggtc acgtcggacg ccaacttgat cggcaccgcg
      901 aacgtgccca acgccttgat cggcttgtcc aagacgatct tgcgtttgtc aaccacgaat
      961 tctttcgcgg ccaacgcgtc ggcgatgtcc ttgctcgtca ccgacccgaa caacttgtcg
     1021 tcctcgcccg cctgcatggc gaacgtcaac ggcagcgacg ccagccgctt gaccagttct
     1081 tcggccgcca cgcgctcctt cttgcgcttg gcctcgatca cgcgcttctc gtgctccagc
     1141 gcatgcacgt tccgctcggt ggcgggcgcc gccatccggc gtggcagcag gaaattccgg
     1201 gcatacccgt ccttgacggt tacgagttcg cccattttgc cgacatgatc cacgtcttca
     1261 cgaagaatga ttctcatggc actcaactcc gatctaagat tgaacgaagg cagaatcata
     1321 acgaaaccgc aaaagggatg tcaacgtgca gcggcgccat ccagatggcg ccgcaggctt
     1381 cttctcaggt agccacgcaa cgcaccttgc caggcgctgg ggaacgcgct aggcttccca
     1441 cgctaatcgc cacggaacat cctcacccag ggggactgcc atgaagagac tcgcgctcat
     1501 cgtgttggga ctctcgctga ccacgcccgt attcgcccag gaacccgttg ccccggtagc
     1561 cgcaccggca tccgacaagg tgttccgcgc cgtgttcacc accgccgtca ccaaccgcga
     1621 acccgcggat caggtcacct cgctcaagag cgacgtccag caggtcttct ttttcaccga
     1681 gctctccggc ctggacggcc agaccatcac gcacaagtgg gaacacgcgg gcgaggtcaa
     1741 ggccgaggtc aaattcgacg tcaaagcccc gcgctggcgc gtgtggtcca gcaagaaatt
     1801 cgacccgagt tggaccggcg agtggacggt gtcggtggtg gatggggcgg gtgcggtgct
     1861 agcgcaggga aagttgatgt atgaggtagc cgggcagtag cgaccgacca gcctctcccg
     1921 aacatgaggg gctgaggaat cacccctcag ataccctgta ctcacaaaat aaatctgtcc
     1981 ccgattcttt acgattcttt aagcccacct tcacgcgggc tccttacatg ccccctcgac
     2041 aagcttttcg tcaccgacct gcgagtgatg caccgacggt aactcgcagt catctaaaat
     2101 cacgaccttt gccgagtaac agtattgttg accagtaacc gcagaacggt aggcgacact
     2161 aagcctaaag ttaaggtgag gggtgctgcc tggtccccgt agtcgtttgt aaacgtccgg
     2221 gaaagagggg tcctcaaatc cgtcagcaat ggattgccct ggggccagta tcagctcgcc
     2281 aggaaccttc aacgacacct ctgccccttt aggagtggcg actagacgat ctcccatgaa
     2341 gacctcgaga tcggatttct cgacgaagat cggaactcca gaaacatttc taaagtaagc
     2401 attgaaacca gaagcaggat tctcacacga cagattcctc ggcgcgaacc actgatactg
     2461 atcgtattta atgagcggta gcgtcgatgc ggaaaatgca tgaccagcct gccgaacgag
     2521 gtcggatgtc tggcagtttt gcacgagcgc caaaaccaac gtagcgaccg cgctacccgc
     2581 tatgatgacg ttggtccatg acaaaactct attttctgtc actaatgcct cgcaattccc
     2641 gctaaacctt tgaacttcta tgatacgagc ac
//
LOCUS       DBNGTT010000231         2023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2604312, whole genome shotgun sequence.
ACCESSION   DBNGTT010000231 DBNGTT010000000
VERSION     DBNGTT010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2604312"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..534
                     /gene="mntA"
                     /locus_tag="AC16BC_05145"
     CDS             127..534
                     /gene="mntA"
                     /locus_tag="AC16BC_05145"
                     /inference="COORDINATES: protein motif:HMM:NF047752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system MntA family
                     adenylyltransferase antitoxin"
                     /protein_id="HIR3241038.1"
                     /translation="MTFPYKDAVIRCVRDRLPDVRAIYRYGSAGTERERPDSDVDIAV
                     LPAAPLSPETRLALSGDLGGVMGRSVDLVDLLAAPTVLRAHIVASGERLFCADERACA
                     VFEDYAFSSYAKFSDERRELVADILGRGSVHGR"
     gene            524..937
                     /gene="hepT"
                     /locus_tag="AC16BC_05150"
     CDS             524..937
                     /gene="hepT"
                     /locus_tag="AC16BC_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system HepT family
                     RNase toxin"
                     /protein_id="HIR3241039.1"
                     /translation="MADDILLAKAAIIERCLKRISEDYRGHEAAFAVEFMRQDAIILN
                     LQRACEAAIDGAMHLVRIGSLGLPQDSRDAFSLLEQAKRLSPDLATRMRAMVGFRNVA
                     VHDYGTLNLDIVRAIVEHRLDDLRAFARVLIQQAG"
     gene            950..1351
                     /gene="mntA"
                     /locus_tag="AC16BC_05155"
     CDS             950..1351
                     /gene="mntA"
                     /locus_tag="AC16BC_05155"
                     /inference="COORDINATES: protein motif:HMM:NF047752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system MntA family
                     adenylyltransferase antitoxin"
                     /protein_id="HIR3241040.1"
                     /translation="MAEYAPKVEKALASVLTRARGDETVLAVILYGSHARGDARPDSD
                     VDVCLLLEPAHYDALTLSEKKLEYLALGGADVQVFQQLPLYIRHRVLKEGKFLFVRDE
                     DRLYEFTFRTIQEWEDFRPYYQRYLDEVARG"
     gene            1344..1769
                     /gene="hepT"
                     /locus_tag="AC16BC_05160"
     CDS             1344..1769
                     /gene="hepT"
                     /locus_tag="AC16BC_05160"
                     /inference="COORDINATES: protein motif:HMM:NF047751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system HepT family
                     RNase toxin"
                     /protein_id="HIR3241041.1"
                     /translation="MVDRERVLGKLDELNRYVEELRAIAPRSFEAYLRVETKRACERL
                     LQISVEAVIDICHLLVAGLRLGVPADEAALFDTLARRGAISEGLARTLKEMKGFRNIL
                     VHEYAAVDDAVVYKAVRDRLADFEAFRREALQILDRSAR"
BASE COUNT          324 a          616 c          705 g          378 t
ORIGIN      
        1 gcgcgcgccg cgtccggcgc ggatgatcgg tccgcttgag tcgcgccggg gcatggtgta
       61 tacaggatgt ctccggcggg cggaaggccg ccgcgcgatc gtggatcgac actcccgagg
      121 atgccgatga cttttccgta taaggacgcc gtgatccgct gtgttcgaga tcggctgccg
      181 gacgtgcgcg cgatctatcg gtacggatcg gcggggaccg aacgcgagcg acccgacagc
      241 gacgtggata tcgcggtgct gcccgccgcg ccgttgtctc ccgagacccg cctggcgctc
      301 agcggcgatc tcgggggggt gatggggcgg tccgtggatc tggtcgacct gctggccgcg
      361 ccgaccgttc ttcgcgcgca cattgtggcc tcgggcgaac ggctcttttg cgcggacgag
      421 cgcgcgtgcg ccgtgttcga ggactacgcg ttttcctcct atgccaagtt cagcgacgag
      481 cggcgggaac tggtggccga tatcctcggg cggggcagcg tccatggccg atgacatcct
      541 gctggccaag gccgcgatca tcgagcggtg cctgaaacgg atcagcgagg attatcgcgg
      601 acacgaggcc gcgttcgccg tcgagttcat gcggcaggac gcgatcatct tgaatctgca
      661 gcgcgcgtgc gaagccgcga tcgacggggc catgcacctg gtccggatcg ggagtctcgg
      721 actgccgcag gacagtcggg acgcgttctc gctcttggag caggccaagc gcctgtcccc
      781 ggatctggcc acgagaatgc gcgcgatggt ggggttccgc aacgtggcgg tgcacgacta
      841 cgggaccctg aacctcgaca tcgtgcgcgc gatcgtggaa catcggctgg acgacctgcg
      901 cgcgttcgcc cgcgtgttga tccagcaggc cgggtgagct ggtgacgtga tggcggaata
      961 cgcgccgaag gtggagaagg cgcttgcgtc cgtcctgacg cgcgcccggg gcgacgaaac
     1021 cgtgctcgcc gtcatcctct acggcagcca tgcgcgcggc gacgcgcggc ccgattcgga
     1081 cgtggatgtc tgtctgttgc tcgagcccgc gcactacgac gcgctgacgc tgtccgagaa
     1141 aaagctggag tacctggcgc tgggcggcgc ggacgtgcag gtctttcagc aattgccgct
     1201 ctacatccgg caccgggtgc taaaggaggg caagttcctg ttcgtcaggg acgaagaccg
     1261 tttgtacgag ttcacgtttc ggacgataca ggaatgggaa gatttccggc cgtattatca
     1321 gcgctacctc gacgaggtcg cccgtggttg accgcgaacg ggtgctggga aagctcgacg
     1381 aactcaatcg ctacgtcgag gaattgcgcg ccattgctcc ccggagtttc gaagcctatc
     1441 tccgtgtgga aacaaagcgc gcgtgcgagc ggttgctgca gatctcggtg gaggcggtga
     1501 tcgatatctg ccatctcctg gtggccgggc tccgattggg cgtgccggcc gatgaggcgg
     1561 ctctcttcga caccctcgcg cgccgcggcg cgatctccga ggggctcgcc aggacgctca
     1621 aggagatgaa gggcttccgg aacattctcg ttcacgagta cgccgcggtc gatgacgcgg
     1681 tggtctacaa ggcggtccgc gaccgtctgg ccgatttcga ggcgtttcga cgcgaggcgc
     1741 ttcaaatcct cgaccgctcc gctcgctgat cgcgctgtgt tcgcttctcc caacttcccg
     1801 cttgagtccg ccgcgcgctt tggtgtataa attttctcgt cacatcgcgt cacggatggc
     1861 gtcctgacgc gccagcccgg actattcatg aaggttcgtc gcgaagccgc gcagacgcca
     1921 gtgaaacggg cgctcggagc cgcgaaggag ggaggcgtac tccttgcagt acgttgaccg
     1981 agtggcgaga ccgcccgttg tagcggcgta tccgcggttg tag
//
LOCUS       DBNGTT010000232         5479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3115405, whole genome shotgun sequence.
ACCESSION   DBNGTT010000232 DBNGTT010000000
VERSION     DBNGTT010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3115405"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="AC16BC_05165"
     CDS             <1..492
                     /locus_tag="AC16BC_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241042.1"
                     /translation="FPLYSYVNDNVSQVHRVGVLGYAPFSLYQHLDTSTGTSDRFLPI
                     YTYSYDAGTTEAQLSVLWPMFVHTSRGGRTTQTGILWWLVHYERPDDAHRDFHILGGP
                     AMAVFRHRVAPERSSVEFNPILPFYYREVTPTASRWMLFGGLIGHTAPVTGPGKWQWL
                     WFL"
     gene            607..1191
                     /locus_tag="AC16BC_05170"
     CDS             607..1191
                     /locus_tag="AC16BC_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3241043.1"
                     /translation="MRLITEHVKPDTFLPRIMKAALALFVEKGIDGTTIKDIAKRAKV
                     SEGALYRHFAGKDELAWHLFSTHLTAFTAHLDETVFAHEAAEERVRAFVSECFKAFDE
                     HQDLFTFLILSEHRELKKFRDRGPHPGHLVLRIIREGQRAGEIAPGDPYVLGSLLLGG
                     IIRVCVVRIYEGITKSLHACEREVSDRLWRMLAP"
     gene            1188..2069
                     /locus_tag="AC16BC_05175"
     CDS             1188..2069
                     /locus_tag="AC16BC_05175"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR3241044.1"
                     /translation="MKRRFLAAMVVTVLSVAALLAWGTAAMSSPAQRRGETVVVLHGL
                     LTSPWHMKRIELALAREGFDVVNWGYDSRAGTIEDHARALHEAVRALPPDRPVHFVGF
                     SLGSLIIRYYLGHYEVPHAVRFVMIGPPNHGSELADFVFPAAWFRALAGSDATSQLRA
                     SNHAFFDALGTPPVDVGIIAGGRGDDSGFSNVLPGDDDGRVSVESARLDGAKDFIVLP
                     HRHVILLVANDAIHQIVRFITSGRFDHDLAADALGSGRAPMSVGTNLPLVGILPVTAT
                     ASRDSNTGRPMAHGDSP"
     gene            2066..>5479
                     /locus_tag="AC16BC_05180"
     CDS             2066..>5479
                     /locus_tag="AC16BC_05180"
                     /inference="COORDINATES: protein motif:HMM:NF006386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HIR3241045.1"
                     /translation="MTTGTYRALLRSWGFQSFLWTQFLGAFNDNVFKIVVSMLAVGAA
                     VGTGGSGDLSFVGAVFILPFLLFSGYAGHLADTLNKRRVLIGTKVIEIAAMVLAVGAL
                     WSGRISWMLAVLFLMAFQSTLFSPAKYGILPEMLPDKDLSRANGLLEMSTFMAIILGT
                     SIGTFLFAAWRDHLNLVGLVLVGIAVAGTLTSFGIARVPTPTASKPIPRNPWSEIIGG
                     LKTLLRDRPLWLTVVGISYFWFLGALFQMDLLLLGKDTMQLDDIHVGLMGTCLALGIG
                     VGSLAAGRLSGDKVELGLVPVGSIGMGACSIWLGAAPHSYGHVSALLAALGFASGLFI
                     VPLNALLQQRSGAHEKGRLIATNNFLNTIGILLASAALWVCRDAVNMEPRTIILTFGL
                     VTLAATAYIVSILPDFLIRFSLWVFTHTIYRIRLVGQEHVPFRGPALLVCNHLSHVDA
                     LLVGACVQRFIRFMVYKPYYELPALNWLFRLMKAIPVSGRNRKEVVEAIERARAELRE
                     GHVVCIFAEGAISRTGNLLPFKRGFEKIMEGLDVPVIPVHLDRLWGSVFSFKDGRFFW
                     KWPTRIPYPVTLSFGKPMPATTSAGEVRQVIQEMSSDVVEHRRDTNDLLHRRFIRVAK
                     RRWFSFCMADSSGRTLSYGQTLVGSLLIARWLAANRPNESMIGLLLPSSIAGALANLA
                     VSLAGRAPVNLNFTAGREALDSAIEQCGIRTIITSRIFLAKAKLEERSGMVFFEDLVK
                     EVGSFHKLAWFLAAALLPSRAVAALADRAAPDPRRLATVIFSSGSTGTPKGVMLSHHN
                     VLSNIESLAQIFWVTRQDRVLGALPFFHSFGFTGTLWFPLLCGFGAVYHPNPLDAKAL
                     GELVATHRATILISTPTFYNAYLRKCTKEQFATIRYAMVGAEKLREPLAAAFRDTYGI
                     DLLEGYGCTEMSPVVAVNVPNVDHHAEHQTGLKPGTVGHPIPGVAARVVDPDTREPLP
                     QGAEGLLLVKGANRMMGYLGQDEKTAEALHDGWYITGDIAAIDEDGFLRITDRLSRFS
                     KIGGEMVPHLQVEEAINQILGDHACVVTAVPDEQKGERLIVLYTRRDVTPDALWTQLS
                     RTDLPKLWIPKRDSFYVIEKIPVLGTGKVDLRAAKAMALTCARPTVNPEE"
BASE COUNT          929 a         1814 c         1700 g         1036 t
ORIGIN      
        1 tttcccctgt acagctacgt caacgacaac gtctcgcagg tccaccgggt cggcgtgctc
       61 gggtacgcgc ccttctcgct ctaccaacac ctggacacct cgaccggaac cagcgaccgc
      121 ttcctgccga tctacaccta ttcatacgac gccggaacca ccgaggccca attgtccgtg
      181 ttgtggccga tgttcgttca taccagccgc ggcggccgca ccacgcaaac cgggattctg
      241 tggtggctcg tgcactatga gcgcccggac gacgcccatc gcgactttca catcctgggc
      301 ggaccggcca tggccgtatt ccgacaccgc gtcgcgccgg aacgctccag cgtggagttt
      361 aaccccatcc tgcctttcta ctaccgcgag gtcacgccga ccgcgtccag gtggatgctc
      421 ttcggcggcc tcatcggcca caccgcgccg gtgacgggtc cggggaagtg gcaatggctc
      481 tggttcctct agcctgccgc ggatggagct ttttgagcgg ctggctgaat gatatgcccg
      541 aagagttgac aaccggatcg aatctcccta tagtgagtga gcattcactt tctacgaggg
      601 ggtttcatgc gcttgatcac cgagcacgtc aaacccgaca cgttccttcc ccggatcatg
      661 aaggccgcgc tcgccttgtt cgtggagaaa ggcatcgacg gcaccacgat caaggacatc
      721 gcgaaacgcg ccaaggtgtc cgagggcgcg ctctatcggc actttgccgg caaggacgaa
      781 ctcgcctggc acctgttctc aacccatttg accgccttca ccgcgcacct ggatgaaacg
      841 gtctttgcgc acgaggcggc cgaagagcgc gtgcgtgcgt tcgtgtccga gtgtttcaag
      901 gccttcgacg agcaccagga cctgttcacg ttcctgattc tctccgagca ccgcgaactc
      961 aagaagttcc gggaccgagg tccccacccc ggtcacctgg tgttgcgaat catccgcgag
     1021 ggtcagcgcg cgggcgagat cgcgcccggc gatccgtacg ttctgggttc gctgctcctc
     1081 ggcggcatca tccgcgtctg cgtggtccgc atctacgaag gaatcaccaa aagcttgcac
     1141 gcgtgcgaac gggaggtgag cgatcgcctc tggcggatgc tggcgccatg aaacgacggt
     1201 tcctggcggc gatggtcgtc accgtgttga gcgtcgcggc gctcctggcg tggggcaccg
     1261 ccgccatgtc gagtccggcc caacgtcggg gcgaaaccgt ggtcgtcctc catgggttgc
     1321 tgacctcacc ctggcacatg aagcgcatcg aactggcctt ggcccgggag gggttcgacg
     1381 tggtgaactg gggctacgac agccgcgcgg gaacgatcga ggaccacgcc cgcgcgttac
     1441 acgaggcggt gcgggccctc ccgcctgatc ggcccgtgca ctttgtggga ttcagcctcg
     1501 ggagcctgat catccggtac tacctcggtc actatgaagt tccccacgcc gtccggttcg
     1561 tgatgatcgg accgcccaac cacggctcgg agctggccga cttcgtattc cccgcggcgt
     1621 ggttccgcgc gctcgcggga tccgacgcca cgtcgcagct tcgcgcctcc aaccacgcgt
     1681 tcttcgacgc gctgggcacc ccgccggttg acgtgggcat tatcgcgggc ggacgaggcg
     1741 acgacagcgg attcagcaac gtgttgcccg gcgatgacga cgggcgcgta tcggtggaga
     1801 gtgcgcggct tgacggagcc aaggacttca tcgtcttgcc ccatcggcac gtgatcctcc
     1861 tcgtcgcaaa cgacgcgatc catcagatcg tcaggttcat cacgagcggt cgattcgatc
     1921 acgacctcgc ggccgacgcg ctcgggagcg ggcgagcccc tatgtccgtc gggacgaacc
     1981 tcccgctcgt tggtatcctc cccgtgacgg ccacggcgtc ccgcgactcg aacacgggac
     2041 gacccatggc tcacggagac tcgccatgac caccggcacg taccgcgcgc tgctcagatc
     2101 ctggggcttc cagtcgtttc tgtggaccca gttcctgggc gcgttcaacg acaacgtctt
     2161 caagatcgtg gtctcgatgc tcgcggtggg cgcggccgtg ggcaccggcg gcagcggcga
     2221 tctgtcgttc gtgggggccg tgttcatcct gcccttcttg ttgttttccg gctacgccgg
     2281 acacctcgcg gacacgctca acaaacgccg cgtgctgatc ggcaccaagg tgattgaaat
     2341 cgccgcgatg gtgctggccg tgggcgcgtt gtggtccgga cggatttcct ggatgctcgc
     2401 ggtgttgttt ctgatggcgt tccagtccac cctgttcagc cccgccaagt acgggattct
     2461 gcccgagatg ttgccggaca aggatctctc gcgcgccaac ggcttgctgg aaatgagcac
     2521 gttcatggcg atcatcctgg gcacgtcgat cggcacgttc ctgttcgccg cttggcgcga
     2581 ccatctcaac ctggtcggcc tggtgttggt cggcatcgcc gtcgcgggca cgttgaccag
     2641 tttcggcatc gccagggttc ccacgccgac cgcgtcaaag cccatccccc gcaatccctg
     2701 gagcgagatc atcggtggcc tgaagacact cctccgcgac cgacccctgt ggttgaccgt
     2761 ggtcgggatc agttacttct ggttcctcgg cgccttgttt cagatggacc tgctgttgct
     2821 gggcaaagat acgatgcagc tcgacgatat ccacgtcggc ctgatgggca cgtgcttggc
     2881 gctcggcatc ggggtgggca gcctggccgc gggccgacta tccggagaca aagtggaatt
     2941 gggcctggtg ccggtgggat cgatcggcat gggcgcgtgt tcgatctggc tcggcgccgc
     3001 cccgcattcc tacggccacg tctcggcatt gctcgcggcg ctgggattcg cgagcggcct
     3061 gttcatcgtg ccgctgaacg cgctgctgca acaacggagc ggcgcccacg aaaaggggcg
     3121 gctcattgcc accaacaatt ttctgaacac gatcggcatc ctgctcgcct cggccgcgtt
     3181 gtgggtctgc cgggacgccg tgaacatgga gccgcggacc atcatcctga ccttcggact
     3241 cgtcaccctc gcggccaccg cgtatatcgt gtcgatccta ccggattttc tgatccgttt
     3301 ctccttatgg gtattcacgc acacgatcta ccgcatccgt ctcgtgggcc aggagcacgt
     3361 cccgtttcgc ggccccgcgt tgctcgtgtg caaccacctc tcgcacgtgg acgcgctcct
     3421 ggtgggcgcg tgcgtgcagc gtttcatcag gttcatggtc tacaagccgt actacgagct
     3481 gcccgccttg aactggctgt tccggctgat gaaggcgatc cccgtgtccg gccgcaaccg
     3541 gaaggaagtc gtcgaagcga tcgaacgcgc gcgcgccgaa ctgcgggaag gacacgtggt
     3601 ctgcatcttt gccgagggcg cgatcagccg caccggcaac ctgctgccgt tcaagcgggg
     3661 gtttgaaaag atcatggagg gcttggacgt gcccgtgatc ccggtgcatc tcgaccggtt
     3721 gtggggcagc gtcttcagct tcaaggacgg cagatttttt tggaagtggc ccacgcggat
     3781 tccctacccg gtcacgttgt cgttcggaaa acccatgccc gccaccacca gcgcgggcga
     3841 agtgcgccaa gtcatccagg agatgagcag cgacgtggtg gaacaccgtc gcgacaccaa
     3901 cgacctgctg caccggcgat tcatccgcgt ggccaagcgc cgctggtttt cgttctgcat
     3961 ggcggactca agcggcagga cgctgagtta tggacagacg ctggtcggca gcctcctgat
     4021 cgcgcgatgg ctcgccgcga accgtcccaa tgaatccatg atcggcctgt tgctcccgtc
     4081 gtcgatcgcg ggcgcgctgg ccaacctcgc ggtctcgctc gccgggcgcg cgcccgtaaa
     4141 cttgaacttc acggccgggc gcgaggccct ggactcagcg atcgagcagt gcggcatccg
     4201 caccatcatc acgtccagga tcttcctggc caaggccaag ctggaagagc gatccggcat
     4261 ggtgttcttc gaggacctcg tcaaggaggt cggctcgttt cacaagctcg cgtggttcct
     4321 cgccgcggca ttgttgccgt cacgagccgt ggccgcgctg gctgaccggg ccgcgcccga
     4381 tccccggcgc cttgccaccg tgatcttttc cagcggcagc acgggcacgc ccaagggcgt
     4441 gatgctctcg caccacaacg tgttgtccaa tatcgagtcg ctcgcgcaaa tcttctgggt
     4501 gaccaggcaa gaccgcgtgc tcggggcgtt gccgttcttc cattcgttcg gcttcaccgg
     4561 cacgctctgg tttccgttgc tctgcgggtt cggcgcggtc taccacccca acccgctgga
     4621 cgccaaggcc ctgggggaac tcgtggcgac ccatcgcgcc accatcctca tcagcacccc
     4681 gaccttctac aacgcgtacc tgcgcaaatg taccaaggaa cagttcgcca cgatccgcta
     4741 tgccatggtg ggcgcggaga aactgcgcga acccctggcc gcggcgttcc gcgacacgta
     4801 cggcatcgac ctcctggaag gatacggttg caccgagatg agcccggtgg tcgcggtgaa
     4861 cgtccccaat gtcgatcacc acgcggagca tcagacagga ctcaaacctg gaacggtcgg
     4921 gcaccccatc cccggcgtgg ccgccagagt ggtggacccc gacacccgcg agccgctgcc
     4981 ccagggcgcc gagggcctgc tgctggtcaa aggcgccaac cggatgatgg gctacctcgg
     5041 ccaagacgag aaaaccgccg aggcattgca cgacggctgg tacatcaccg gcgacatcgc
     5101 ggccatcgac gaggacgggt tcctccggat cacggaccgg ctctcgcggt tctccaagat
     5161 cggcggcgaa atggtcccgc acctccaagt cgaggaagcg atcaaccaaa tcctcggcga
     5221 tcacgcctgc gtggtgaccg cggtgcccga cgaacaaaag ggcgaacgat tgatcgtgct
     5281 ctacacccgg cgcgatgtca caccggacgc gttgtggacg caactctccc ggacggattt
     5341 acccaaactc tggattccga agcgggacag cttttacgtg atcgagaaga ttccggtgtt
     5401 gggcaccggc aaggtggatc tgcgagccgc aaaggccatg gcgctgacgt gcgccaggcc
     5461 tacggtcaac cctgaggaa
//
LOCUS       DBNGTT010000233         2821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1326472, whole genome shotgun sequence.
ACCESSION   DBNGTT010000233 DBNGTT010000000
VERSION     DBNGTT010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1326472"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="AC16BC_05185"
     CDS             <1..819
                     /locus_tag="AC16BC_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241046.1"
                     /translation="RLNPTHPHTWAFADQPLVNGRMFGGDGMRGPGLQLSYLVPLPIY
                     TEAMLALQNSQGETMFSFRNAPEEDLFDRTLIERRVEGLDDLVIAPRVAASINPSDTQ
                     TVLFGVSGAFGPNGSGESARTRIYGADLYYKWKPLRTVKGWPFVSWQTEVMTRRYVNA
                     AVADDPATLPDETAPEEVLRDWGAYSHLVWGFTPRWAAGLRGDYAGGRGGDLTDPLRE
                     RRVRVSPNLTFSPSEFSKWRIQYNWDHVPTREDAVHSVFVQWEFLVGTHGAHAF"
     gene            842..1768
                     /locus_tag="AC16BC_05190"
     CDS             842..1768
                     /locus_tag="AC16BC_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR3241047.1"
                     /translation="MKRIGLVVLFVAALVNPASGGPLSVVATTTDLAAIAKAVGDVRV
                     DVASLATGVQDPHFIDPRPSFLVKLNRADLLIDGGLDLEVGWLPALLDSARNPRLHIG
                     EPGRLDASQGIRVLGVPTGSVDRSQGDVHGKGNPHYLVDPMNGVIVADTIAKRLCRLD
                     AAGCDVFTSNAQAFRTRIETGLSKWTKSLAPYRGAKIVVYHDSWPYFTKRFALNTVGY
                     VEPKPGVPPPPSHVSALTDLMRREQAKIIMMEPYHERSIPDLLARQTGATVLVLAPTV
                     GATPGTDDYVALFDTDVTRLVEALSAGGPAAK"
     gene            1765..2547
                     /locus_tag="AC16BC_05195"
     CDS             1765..2547
                     /locus_tag="AC16BC_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3241048.1"
                     /translation="MIEMLALPFMRQAALACVLLAFVLAPLGAEVIRRRIVFVDLALA
                     QIAALGAAIGTAVEWDPIATSLVFTVIGAALLSVPAATTRLPQEAVMGMVYAAASAAA
                     VLIVAKTPHGDADFLHLLFGNILAVTDRQVIVLAGLAVVVAIWRLAARRSDPASIGRG
                     ATVVFYLLLGGAIAAAIRSAGVLMVFTYLVAPALAMRLVGLERGAGAAAVASAAACGV
                     LGLWGSYRWDLPTGSSIVAMFGVGLIVTAFAASLRRRTGAGA"
BASE COUNT          430 a          875 c          975 g          541 t
ORIGIN      
        1 cggctcaacc ccacgcatcc gcatacctgg gcgttcgcgg accaaccctt ggtcaatggc
       61 cgcatgtttg gaggcgacgg gatgcggggc cccgggctcc aactgtcgta tctggttccc
      121 ctgccgatct ataccgaggc gatgctggcg ctccagaaca gtcagggcga gaccatgttc
      181 agttttcgca atgcgccgga ggaggatctc ttcgatcgga ccctgattga gcggcgggtc
      241 gaagggctgg acgatctcgt gattgccccg cgcgttgccg cctccataaa tcccagcgac
      301 acccagaccg tgctgttcgg ggtttcaggg gcgtttggcc ccaacggcag cggagagtcg
      361 gcgcggaccc ggatctatgg ggccgacctg tactacaagt ggaaacccct gcgaaccgtg
      421 aagggctggc cgttcgtctc gtggcaaacc gaggtgatga cgcgccgcta cgtcaatgcc
      481 gcggtggcgg acgacccggc gactcttccc gacgagaccg ccccggagga agtgctccgc
      541 gactggggcg catactccca cttggtctgg ggatttacgc cgcgctgggc ggccgggttg
      601 cgcggagact acgcgggggg gcgcggcggc gacctcaccg accccctgcg cgaacggagg
      661 gttcgcgtgt ccccgaacct gacgttttct cccagcgaat tctccaagtg gcggattcag
      721 tacaactggg accacgtgcc gactcgcgaa gacgcggtgc actcggtatt cgtgcagtgg
      781 gaattcttgg tcggcaccca cggcgcgcat gcgttttaac tcaaccgaga ggagagccct
      841 catgaagcgc atagggttgg tggtgttgtt cgtcgcggcg ttggtcaatc ccgcgtcggg
      901 cgggccgctg tcggtcgtcg cgaccaccac ggatctggcg gcgatcgcca aagccgtggg
      961 cgacgtccgc gtcgacgtgg ccagcttggc tacgggtgtt caggatccgc attttatcga
     1021 cccgcggccc agttttctgg tcaagctgaa ccgagccgat ctcctgattg acggcgggtt
     1081 ggatttggaa gtcgggtggc tgcctgcgtt gctcgacagc gccagaaatc cgcgtcttca
     1141 tatcggagag cccggccgcc tcgacgcgtc gcaggggatc cgcgtgctcg gcgttcccac
     1201 gggatcggtt gaccgctccc agggcgacgt ccacggcaag gggaatcccc actatctcgt
     1261 ggacccgatg aacggcgtca tcgtggcgga cacgatcgcg aagcggctgt gccgcctcga
     1321 tgcggccggc tgcgacgtgt tcacgtcgaa cgcgcaggcg ttccgcactc gaatcgaaac
     1381 cggcttgagc aagtggacga agtccctcgc gccgtaccgg ggagccaaga tcgtcgtgta
     1441 ccacgatagt tggccctatt tcaccaaacg attcgcattg aacaccgtcg gatacgtcga
     1501 gcccaagccg ggcgtgcctc cgccgcccag ccacgtgtcg gcgttgacgg atctcatgag
     1561 gcgcgaacag gcgaaaatca tcatgatgga gccgtaccac gagcggagca tcccggacct
     1621 gttggcccgg cagaccggcg ccacggtgct ggtgctggcg ccgaccgtgg gagccacgcc
     1681 ggggacggac gactacgtcg cgctcttcga cacggacgtg acgcgcctcg ttgaggcgct
     1741 cagcgcggga ggcccggctg cgaaatgatc gagatgctgg ctctcccgtt catgcggcag
     1801 gcggccctgg cctgcgtgct gttggcgttc gtgctggctc ccttgggcgc cgaggtgatc
     1861 cggcggcgga tcgtgttcgt ggacctcgcg ttggcgcaga tcgcggcgct cggcgcggcc
     1921 atcggcaccg cggtggagtg ggatccgatc gcgacgtcgc tcgtcttcac cgtgatcggc
     1981 gccgcgctcc tgtccgtgcc cgctgcgacc acgcgcttgc cgcaggaagc cgtgatgggc
     2041 atggtctacg cggccgcgtc cgcggccgcg gtgctgatcg tcgccaagac cccgcacggc
     2101 gacgcggact ttcttcatct gctgttcggc aacatcttgg ccgtgaccga ccgtcaggtc
     2161 atcgtattgg cggggctggc ggttgtcgtg gcaatctggc ggttggcggc ccgtcgcagc
     2221 gatccggcct cgatcggccg cggcgccacg gtcgtattct acctgctgct tggcggagcc
     2281 atcgcggccg cgattcggag tgcgggcgtg ttgatggttt tcacgtattt ggtcgcgccc
     2341 gcgttggcga tgcggctcgt tggtctggag cgcggagccg gtgccgccgc ggtggcgtcc
     2401 gccgccgcgt gcggggtgct cggtctgtgg ggatcgtacc gatgggattt gccgacgggt
     2461 tcatcgatcg tggcgatgtt tggggtggga ctcattgtga cggcgtttgc ggcgtcgctc
     2521 agaaggcgaa ccggcgcagg ggcataagcc cattcacagg gggatcaagg gtcgttgtcc
     2581 cggcgagggt gaaggcttct ggatgttttg tcagcggtcg gctagacgtg ccatgcccgc
     2641 tatgttagat tgtgagcccg atgtctgaaa ccccgctgct catcatcgct gcgagcgaga
     2701 tcgactccaa tctgtattac gcgacgcggt ttctcgcgcc cgacgccttc gtgtatttgc
     2761 gcgatcggga ccaatccatg ctcctgatga gcgacctgga gatggatcgc gcgaaggcgc
     2821 a
//
LOCUS       DBNGTT010000234         3541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2094420, whole genome shotgun sequence.
ACCESSION   DBNGTT010000234 DBNGTT010000000
VERSION     DBNGTT010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2094420"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..435
                     /locus_tag="AC16BC_05200"
     CDS             229..435
                     /locus_tag="AC16BC_05200"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR3241049.1"
                     /translation="MPEDDAFRKGLALAGRVGVELVASTIVGAGLGYGLDRWLGTGPW
                     LMIVGLLLGAAAGVLGIIRLVKDS"
     gene            439..1164
                     /locus_tag="AC16BC_05205"
     CDS             439..1164
                     /locus_tag="AC16BC_05205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3241050.1"
                     /translation="MENPLHHFELHPWIPLSLFGIDISINKAVVMMWIASGLVFLFFY
                     VATQSRSLVPRRIQSLAEMAVEFLRGMVVENMGPLGHKFFPFIATLFFFVLFANLLGL
                     IPGAYTVTSQLMVTGFFALIVYGISLIVGFALHGTHFLSILAPAGTPGWLLPLMVPIE
                     IISQLARPVTLAVRLFANMTAGHIILIVLFSLMMTAPIYLGWLPFGFTVAIYGLEVFV
                     AFIQAYIFTILACVYLGDAVKLH"
     gene            1198..1428
                     /gene="atpE"
                     /locus_tag="AC16BC_05210"
     CDS             1198..1428
                     /gene="atpE"
                     /locus_tag="AC16BC_05210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR3241051.1"
                     /translation="MDASAAALLGMGIAAAGFGGAGIGIGYIFGKMIESTARQPAAEA
                     KVGKYMWIGFALVEAIALYGLVIAFIIMGLRG"
     gene            1575..2066
                     /gene="atpF"
                     /locus_tag="AC16BC_05215"
     CDS             1575..2066
                     /gene="atpF"
                     /locus_tag="AC16BC_05215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF017242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR3241052.1"
                     /translation="MPQFDTHFLTPLLFWSAVSFAILLFLLKRYAFPAITEMLDARER
                     TIAQNLAKAEQARKEAEQLLAEYQAKLKAAQQEATAMLDQARKQGQQLADETQRRVKE
                     ETDRALAASRDELARERLRLSKELREQAVDLVLAATERVVRRNLTDADSKRLVKEAIE
                     QLN"
     gene            2090..2809
                     /locus_tag="AC16BC_05220"
     CDS             2090..2809
                     /locus_tag="AC16BC_05220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3241053.1"
                     /translation="MPRVRAVLLDLCDTLVHFDAERLPLVEIDQQPTRSTALAIYDAI
                     GPSPVPFDQFADCLKTVTAEIATQRDIDHREIASQERFRRVLDRLGLPASPDHAQRLV
                     DAHMERLAHALVTPAHHREVLAGLAGRVRLGIVSNFDHAPTVREVLRRDGLEEFFDVV
                     IVSDEIGWRKPHRVMFETALQRVGVSSGEALFVGDNYELDVLGAIQVGLSAAWYARGR
                     PVDREIGHPIIADLADIIHLL"
     gene            complement(2921..>3541)
                     /locus_tag="AC16BC_05225"
     CDS             complement(2921..>3541)
                     /locus_tag="AC16BC_05225"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF014610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA sulfurtransferase"
                     /protein_id="HIR3241054.1"
                     /translation="GGLDSPVAAYRMMKRGAYVVFVHFHSHPFVSRASQEKAKELAEL
                     LTRYQYASRLFLVPFGEVQREVVLAVPGPLRVIVYRRLMIRIAEAVARTVRAQALVTG
                     EALGQVASQTLSNMTTVDAVATMPVLRPLVGMDKEEIVHQARALGTYEISIQPDEDCC
                     TLFTPKHPATASTPAEVEAAEAKLDIPALIQMSMEKLEEARFAFPA"
BASE COUNT          624 a         1101 c         1101 g          715 t
ORIGIN      
        1 gtgcgctgta tccatagggt tctccgacgt tgtgggcgag ccgcgcgtgg ggaatccgga
       61 acggtacatc cccgaagtgc atatcggccc gtatgctaaa ccactccttc cccgcgtgtc
      121 aatgaacaac cggacgaatc gtccggcgag acccgcctcg tgatgtaact ctcggcttga
      181 ctcaccgagc gaatttccgg tacagtaccg gcctttgaga ggcgccagat gcccgaagac
      241 gacgcgttcc gtaaggggct cgcgcttgcg ggccgggtcg gggtggagct ggtcgcctcg
      301 accattgtgg gcgccggttt gggttacgga ttggaccgct ggttgggcac cgggccgtgg
      361 ttgatgatcg tgggcctgct tctgggcgcg gccgccgggg tgctcggaat cataaggttg
      421 gtcaaagact cctagacgat ggaaaatccg cttcaccatt ttgaattgca cccgtggatc
      481 ccgctgtcgc tgttcgggat cgacatctcg atcaacaaag cggtcgtgat gatgtggatc
      541 gcgtccgggc tggtgtttct ttttttttac gtggccacgc agagccgaag ccttgtccca
      601 cgccgtatcc aaagccttgc ggaaatggcc gtggagtttc tgcgcggcat ggtggtcgag
      661 aatatgggcc cgctcggcca caagttcttt ccgttcatcg ccacgctgtt tttcttcgtg
      721 ctgttcgcga atctcttggg tttgatcccc ggcgcctata ccgtgaccag ccagttgatg
      781 gtcacgggat ttttcgccct gatcgtgtac ggcatcagcc tcatcgtagg tttcgcattg
      841 cacggaacgc actttttgtc gatcctcgcg cccgccggta ccccggggtg gctcctgccg
      901 ctgatggtgc cgattgaaat catcagccag ctcgcccggc ccgtcacgct cgcggtccgg
      961 ctcttcgcga acatgaccgc ggggcacatc atcttgatcg tgctgttttc gttgatgatg
     1021 accgcgccga tctatcttgg ttggttgccg ttcggattta ccgtcgccat ctacggactg
     1081 gaagtattcg tggcgtttat ccaggcgtac atttttacga tcttggcgtg cgtgtacttg
     1141 ggtgacgccg tcaaattgca ttaaccgcat ttcatagaga agagaaggag aagacgaatg
     1201 gatgcatcgg cagcagcgtt gttgggcatg ggaatcgcgg cggcgggatt cggcggcgcg
     1261 ggtatcggca tcggctacat cttcggcaaa atgatcgaat ccacggcgcg ccagcccgcg
     1321 gccgaagcca aggtcggcaa gtacatgtgg atcggcttcg cgttggtgga agccatcgcg
     1381 ttgtatggct tggtcatcgc gttcatcatc atgggtctgc gcggataaga gcccgtccat
     1441 gagcggccat ctgcggcgtt gccgctgcgc atccggtgct cacgtaccga acttcgtacg
     1501 ctccgctccg gtgctcgcgg cgcctggcat ctggcgcgct cctgaacagg ctccctgtcc
     1561 tggagtaaaa tgtcatgcct cagtttgata cccacttcct gaccccgctg ctgttctggt
     1621 ccgcggtttc gttcgcgatc ctgctgttcc tgctgaagcg gtatgcgttt cccgcgatta
     1681 cggagatgct ggacgcgcgc gagcgcacca tcgcgcaaaa cctggccaag gccgagcagg
     1741 cgcgcaaaga ggccgagcag ttgctggccg agtatcaggc caagctcaag gccgcccagc
     1801 aggaagccac ggccatgttg gatcaggccc gcaagcaggg ccagcagctt gctgacgaga
     1861 cgcagcgaag ggtcaaagaa gaaaccgacc gcgcgctggc cgcgtcgcgc gacgagttgg
     1921 cgcgcgaacg cctgcggctc agcaaggaat tgcgcgagca agccgtggac ctggtgttgg
     1981 ccgccaccga gcgcgtggtg cgtcgcaatc tgaccgacgc ggattccaaa cggctggtca
     2041 aagaggcgat cgagcagttg aattaacgcg cggggcgagg ccctgagcca tgccccgcgt
     2101 gcgcgccgtt ctcctcgacc tctgcgacac gctcgtgcat tttgacgccg agcgcctgcc
     2161 cctcgtcgag atcgaccaac agcccacgcg atcgaccgcc ttggccattt acgacgcgat
     2221 cggcccgtcg ccggtcccgt tcgatcaatt tgccgattgc ctcaaaaccg tcaccgccga
     2281 gatcgcaacc caacgcgaca tcgatcaccg cgaaatcgcg tcgcaggagc gctttcgccg
     2341 ggtgctggat cggctcggac tgccggcaag tcctgaccac gcgcaacgcc tggttgacgc
     2401 ccacatggag cgattggccc acgcgttggt cacacccgca catcatcgcg aggtcttggc
     2461 cgggctggcc ggtcgcgttc gactcggcat tgtctccaac ttcgaccacg cgcccaccgt
     2521 gcgcgaggtg ctcagacgcg acgggttgga agagtttttt gacgtggtga tcgtctcgga
     2581 cgagatcggt tggcgaaaac cccaccgcgt catgttcgaa accgcgctgc aacgggtggg
     2641 agtctcttcc ggcgaggcgc tcttcgtggg ggacaactac gagttggatg ttctgggcgc
     2701 gatccaggtg ggcctctccg ccgcgtggta cgcgcggggg aggccggtcg accgcgagat
     2761 cgggcatccg atcatcgcgg acctcgcgga cattatccat ttgctttaac cggttgcggg
     2821 aaaagtcaaa ggcgtggttc gacaagctcg ccacgaacgg aacttcccct gcaacctcta
     2881 tcttcgatcc gttcgtcctg agcctgtcga agggcctcca ctacgcggga aacgcgaacc
     2941 gcgcctcttc cagcttctcc atcgacatct gaatcaacgc ggggatgtcg agttttgcct
     3001 cagccgcttc gacctcggcc ggcgtggatg cggtcgcagg gtgcttgggc gtgaacaagg
     3061 tgcagcaatc ctcgtcgggc tggatcgaaa tctcgtacgt gcccagcgcc cgcgcctggt
     3121 gcacgatctc ctccttgtcc atacccacga ggggcctgag caccggcatg gtggccacgg
     3181 cgtccacggt ggtcatgttc gataaggtct gcgacgccac ctgccccagc gcctcacccg
     3241 tgaccaacgc ctgcgcgcgc accgtgcggg cgacggcctc ggcgatccga atcatgagcc
     3301 gacgatacac gatcacgcgc aagggccccg gaacggccag cacgacctcc cgctggacct
     3361 cgccgaacgg gaccaggaac aaccgcgagg cgtactggta cctggtgagc aactcggcca
     3421 gctccttggc cttttcctgc gaggcgcggg acacgaatgg atggctgtgg aagtgcacga
     3481 acaccacata cgccccgcgc ttcatcatac gatacgcggc caccggggaa tcgagaccgc
     3541 c
//
LOCUS       DBNGTT010000235         5263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1071578, whole genome shotgun sequence.
ACCESSION   DBNGTT010000235 DBNGTT010000000
VERSION     DBNGTT010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1071578"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1313
                     /locus_tag="AC16BC_05230"
     CDS             210..1313
                     /locus_tag="AC16BC_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3241055.1"
                     /translation="MSTLYQLLQQMIEKGASDLHITTGTPPQMRLHGHLVPMNSPALG
                     PAETKQLIYSVLTDAQKHRFEEENELDLSFGLKGLSRFRANVFMQRGAVAAAIRVIPI
                     QTRTLEELGLPQVVADLIKRPRGLILVTGPTGSGKSTTLAAMIDKINSERSDHIITIE
                     DPIEFIHAHKKSLVNQREVTSDTKSFKAALKYILRQDPDVVLVGEMRDLETIEAALTI
                     AETGHLTLATLHTNSCAQTINRVVDVFPPHQQPQVRAQLSFVLEGVLSQQLIPRANGQ
                     GRVMAMEIMVPNPAIRNLIREDKVHQIYSTMQTGQNKFGMQTMNQSLFDFYGKRLITY
                     DEAITRSSVPDELISMMDRAGLTQGQRRVGVVK"
     gene            1344..2552
                     /locus_tag="AC16BC_05235"
     CDS             1344..2552
                     /locus_tag="AC16BC_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3241056.1"
                     /translation="MATTFVWSGKTRQGEAQKGELVAQSREDAIAQLRRQNILATSVQ
                     PKGADKQATPRFGGGVPEKDIVIFTRQFSTMIDAGLPLVQCLEILSNQTENKTLAKTI
                     GLVRADVEGGSTYADALRKFPKVFDDLYVNMVAAGEAGGILDTILSRLAKHIEKSMKL
                     KKQVKSAMVYPGIIISVAVIVISVLMVWVIPIFASMFTDFGGVLPLPTRIVMGMSHFM
                     KSYILLLIAGGVGIGYGIKRYYGTDGGRRLIDRNALKVPVLGDLIRKAAVAKFTRTLG
                     TLISSGVPILDGLTIVAKTSGNKTVESALLTARQSISEGKTVSEPLGQTKVFPPMVVQ
                     MISVGETTGALDAMLSKIADFYDDEVDSAVGALTSLLEPMLMVFLGVTIGFIVIAMYM
                     PIFQMGSVVG"
     gene            2590..4290
                     /locus_tag="AC16BC_05240"
     CDS             2590..4290
                     /locus_tag="AC16BC_05240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3241057.1"
                     /translation="MNAETIRTKLRWVIGLRVVVVTVFLGLFLALNPTASGDPTSRLF
                     GLLIVGTYTLTIAYALALKWIAPRWDPLFAVVQIAGDLACITTVVATTGRIDSPFTFL
                     YPIVILTGTMVLGRTGGLVATVAAGSIYAVLAWPSTATMDLASWWAQRSGVAPVALRV
                     LAFLAVFLLGDHLVETATAATRRLHERESGYRALSAFHENIVQSMSSGLVTADLDGRV
                     TSFNPAGYAMLGLSPDEVLGRPCWDLFNWEDGAGFYARVAQRNVPYRFEREVVRRDGR
                     RVLLGMTLSWLKDSNGEPMGMVGIFQDLTEIKALEDKVRQRERLASVGELAAGLAHEI
                     RNPLAAISGAMEVLQQDLRLEGEPGTLMGIALRETERLDDLIGQFLLYARPPSPQKRY
                     CDLVRLVKETLVLLRTHNDFRPAIEVAEQLPDAPVWVEADANQIRQVVWNLLVNALQA
                     MPDGGRLSVRLRAVAAKRSNAVALTIGDTGQGVKSEDLHRMFLPFFTTKPGGSGLGLA
                     IVHRIVEEHRGHVDVQSEWTKGTQVTVTLPAATPDAAGPGDSESAAEAAILQAEVSRP
                     "
     gene            4287..>5263
                     /locus_tag="AC16BC_05245"
     CDS             4287..>5263
                     /locus_tag="AC16BC_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3241058.1"
                     /translation="MTTGSQNEETGINVFRVLIVDDEKSMREFLSIMLRKEGYAVTVA
                     AHGPEALGLIDKEIYDLVISDVKMPGLSGIDVLKAVKAATPSTVVLMMTAFASTETAV
                     EAMREGAYDYLTKPFKIDEVKLIVKNALERKQLREENSRLKQVVHDQSAFEQIVGRSD
                     KLTKVLDLIRKVADTNSNVLIFGESGTGKELVARAIHYNSRRRERPFVTVNCSALPEP
                     LLESELFGHMKGSFTGAISNKEGLFEIANDGTIFLDEIGETPLGIQVKLLRVLQEKEF
                     RRVGGTRDMKIDVRIVAATNRDLEKSIAEGRFREDLYYRLDVIPINLPPL"
BASE COUNT         1020 a         1572 c         1760 g          911 t
ORIGIN      
        1 gccggtgggc gaggggatcc gtgaactcat ccttcagggc ggcacggcgg acgagatcaa
       61 gcgcaaagcg atcgagagcg gcatgaagac gctgcgcatg gcggggttgc agaaggtgcg
      121 ggagggattg accacagtcg aagaggtcgt cggctcgacg ttcgcggact gacgatggag
      181 atgtcgccga acagggaggt tcacgcataa tgtccacgct ctaccagttg ctgcaacaga
      241 tgattgagaa gggcgcttcg gacctccata tcacgaccgg gactccccca cagatgcggt
      301 tgcacgggca tcttgttccg atgaactctc cggcactcgg accggccgag accaagcagc
      361 tgatctacag cgtgctgacc gatgcccaga aacatcggtt cgaagaagag aacgaactcg
      421 atctgtcgtt cgggctcaag gggttgagcc gcttccgggc gaacgtgttc atgcagcgag
      481 gcgcggtggc ggccgcgatc cgggtcattc ccatccagac cagaaccctg gaggagctgg
      541 gcttgccgca ggtggtggcc gacttgatca agcgtccacg cgggctcatt ctcgtgaccg
      601 gcccgaccgg gagcgggaaa tccacgaccc tggcggccat gatcgacaag atcaattcgg
      661 aacggagcga ccacatcatc acgatcgaag acccgatcga gttcatccac gcccataaaa
      721 agtcgctcgt gaatcagcgg gaagtcacgt cggacaccaa gagtttcaag gcggcgttga
      781 agtacatcct gcgccaggac cccgacgtgg tgctggtggg agaaatgcgc gacctggaga
      841 cgatcgaggc cgcgctgacc atcgcggaga ccggccacct cacgctggct acgctgcaca
      901 ccaacagttg cgcgcagacc atcaaccgcg tcgtggacgt gtttcctccc catcagcagc
      961 cgcaggtgcg cgcacagctg tcattcgtgc tcgagggcgt gctgtcccaa cagttgatcc
     1021 ccagggcgaa cggccagggg cgggtgatgg ccatggagat catggtgccg aacccggcga
     1081 tccgcaatct gatccgcgag gacaaggtcc atcagatcta ctcgaccatg cagacgggcc
     1141 agaacaagtt cggcatgcag acgatgaacc aatccctgtt cgacttttac ggcaagcgcc
     1201 tcatcacgta cgacgaggca atcacgcggt caagcgttcc cgatgagttg atatccatga
     1261 tggaccgcgc ggggctcacc cagggccagc ggcgggtcgg cgtggttaag tagcccggag
     1321 cgggctgagg aggcggaaaa cccatggcga cgacattcgt ctggagcggc aagacccgcc
     1381 agggcgaggc ccagaagggc gagctggtgg cccagagccg ggaagacgcg attgcccagc
     1441 tgcggagaca gaacatcctc gcgacgtcgg tgcaacccaa gggcgcggac aagcaggcga
     1501 cccctcgctt cggcggaggc gttcccgaaa aggacatcgt gatcttcacc cggcagttct
     1561 ccaccatgat cgacgccggg ttgcctctcg tgcagtgcct ggaaattctg tccaaccaga
     1621 ccgagaacaa gacgctggcc aagacgatcg ggctcgtgcg cgccgatgtc gaaggcgggt
     1681 cgacctatgc cgacgcgctc cgcaagttcc ccaaggtctt cgacgatctc tacgtcaaca
     1741 tggtcgccgc gggcgaagcg ggcggcattc tcgacacgat cctgagccgt ctggccaaac
     1801 acatcgagaa atcgatgaaa ctcaagaagc aggtcaagtc cgcgatggtc tatcccggga
     1861 tcatcatcag cgttgcggtg atcgtgatca gcgtgttgat ggtgtgggtc atcccgatct
     1921 tcgccagcat gttcaccgac ttcggcggag tgttgccgct gccgacgcgg atcgtgatgg
     1981 ggatgagcca cttcatgaag agttacatcc tccttcttat cgcggggggc gtgggcatcg
     2041 ggtacgggat caagcgctat tacggcaccg acggcgggcg gaggctcatc gaccggaacg
     2101 cgctgaaagt ccccgtgttg ggcgatttga tccgcaaggc cgcggtcgca aagttcacgc
     2161 gcaccctggg aaccctgatc tccagcggcg ttcccattct ggacggcttg acgatcgtgg
     2221 cgaagacgtc gggaaacaag acggttgaat cggcactgct gaccgcccgg cagagcatca
     2281 gcgaaggcaa gacggtctcc gagccattgg ggcaaaccaa ggtgttcccg cccatggtgg
     2341 tccagatgat ttccgtgggc gagacgaccg gggcgttgga cgcgatgttg tcgaaaatcg
     2401 ccgactttta cgacgacgag gtggattccg ccgtgggcgc gctgacgtcg ctgcttgagc
     2461 cgatgttgat ggtgttcctg ggcgtgacga tcgggttcat cgtaatcgcg atgtacatgc
     2521 cgatcttcca gatgggctcc gtggtgggat aacgcgcgcc gcaccggggc ctgttgcggg
     2581 atctcgatcg tgaacgcgga gacgatccga accaagcttc ggtgggtgat cggtctccgg
     2641 gtggtggtgg tcaccgtatt cctggggctg tttctggcgc tcaaccccac ggcctcggga
     2701 gacccgacat cccgtctgtt cggcctgttg atcgtcggga cgtatacgct gacgatcgcg
     2761 tatgcgctcg ccctcaaatg gatcgccccg cgttgggatc ccctgtttgc cgtcgtgcag
     2821 atcgcgggcg acctggcgtg catcacgacc gtggtcgcca cgaccggacg gatcgacagc
     2881 ccgttcacgt tcctgtatcc gatcgtgatt ctgaccggca ccatggtctt gggccgcacc
     2941 ggcggcctcg tcgccaccgt ggcggccgga tcgatctacg ccgtgctggc gtggccgtcc
     3001 acggcgacca tggacctcgc gtcgtggtgg gcgcagcgga gcggcgtcgc tccggtcgcg
     3061 ctgcgggtct tggccttcct cgcggtgttc ctgctggggg atcacctggt cgaaaccgcg
     3121 accgccgcca cgcgccggct ccacgaacgt gagagcgggt atcgcgcgct ttccgcgttc
     3181 cacgagaaca tcgtccagag catgagcagc ggactggtga ccgccgacct cgacggccgc
     3241 gtgacgtcgt tcaaccccgc gggatacgcg atgctggggc tgtctcccga cgaggtgttg
     3301 gggcgtccgt gttgggatct cttcaactgg gaggatggcg ccgggtttta cgcgcgcgtc
     3361 gcgcagcgca acgtgccgta ccggtttgaa cgggaagtcg ttcggcggga cggtcggcgc
     3421 gtcctgctcg ggatgacgct ctcgtggttg aaggactcga acggcgagcc gatggggatg
     3481 gtcgggattt tccaggacct gaccgagatc aaggcgttgg aggacaaagt ccgccagcgc
     3541 gaacgactgg ccagcgtggg cgaactcgcc gcggggctgg cccatgaaat ccgcaacccc
     3601 ctggccgcga tcagcggcgc gatggaggtg ctgcaacagg acctccgcct ggagggcgag
     3661 cccgggacgt tgatgggcat cgccctccgc gagaccgaac gcctcgacga tctgatcggc
     3721 caattcctct tgtacgcccg cccgccctcg ccgcagaaac ggtattgcga ccttgtccgg
     3781 ctggtcaaag agacgctggt gttgcttcgc acgcacaacg atttccggcc ggcgatcgag
     3841 gtcgccgagc agttgcccga cgcgccggtc tgggtcgagg ccgacgccaa tcagatccgt
     3901 caggtggtgt ggaatctcct ggtcaacgcc ctgcaggcga tgcccgacgg cggccgactc
     3961 tcggtccggc ttcgcgcggt ggccgcaaag cgttcgaacg cggtggcgct gaccatcggg
     4021 gatacgggac aaggcgtgaa atccgaggat ttgcaccgca tgtttctgcc gttcttcacc
     4081 accaagcctg gcgggtccgg gctggggttg gcgatcgtgc accggatcgt ggaggaacat
     4141 cgcgggcacg tggacgtgca gagcgaatgg accaagggca cccaggtcac ggtgaccctg
     4201 cccgccgcga cgccggatgc cgcggggccc ggtgactcgg agtccgccgc ggaagccgcc
     4261 atcctgcagg cggaggtgtc gcgaccgtga caaccggttc acagaacgag gagacgggga
     4321 tcaacgtgtt tcgagtactg atcgtcgacg acgaaaagag catgcgggaa tttttgtcga
     4381 tcatgttgcg aaaagaaggc tacgccgtga ccgtggccgc gcacggtccc gaggccctgg
     4441 ggctgatcga caaggagatc tacgacctgg tgatttccga cgtgaagatg ccgggcttgt
     4501 ccgggatcga cgtgctcaag gccgtgaaag ccgcgacgcc gtcgacggtg gtgttgatga
     4561 tgaccgcgtt tgcgtccacg gaaaccgcgg tcgaggcgat gcgggaaggc gcgtacgatt
     4621 atctgaccaa accgttcaag atcgacgaag tcaagttgat cgtgaaaaac gcgttggagc
     4681 gcaagcagct ccgggaggag aacagccggc tcaagcaggt cgtgcacgac cagtcggcgt
     4741 tcgagcagat cgtcgggcgc agcgacaagt tgaccaaggt cttggacctg atccgcaagg
     4801 tggcggacac caacagcaac gtgctgatct tcggcgagag cgggaccggc aaggaactgg
     4861 tggcgcgggc gattcactac aacagccgtc ggcgcgagcg accgttcgtc acggtcaatt
     4921 gcagcgcgtt gccggagccg ctcctggaga gcgagctgtt cggccacatg aaggggtcct
     4981 tcaccggcgc gatcagcaac aaggaagggc tgttcgaaat cgccaacgac ggcacgattt
     5041 tcctggatga aatcggggaa accccgctcg ggatccaggt caaactcctg cgcgtgctgc
     5101 aggaaaagga gttccggcgc gtgggcggca cccgcgacat gaaaatcgac gtccgcatcg
     5161 tggccgccac caatcgggac ctggaaaaat ccatcgccga ggggcggttc cgcgaggatc
     5221 tctactaccg gctggacgtg attcccatca acttgccgcc gct
//
LOCUS       DBNGTT010000236         6954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2604993, whole genome shotgun sequence.
ACCESSION   DBNGTT010000236 DBNGTT010000000
VERSION     DBNGTT010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2604993"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="AC16BC_05250"
     CDS             <1..518
                     /locus_tag="AC16BC_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3241059.1"
                     /translation="TDVRGTTSVPGLFAVGEAACTGVHGANRLASNSLLEGLVFGTRA
                     GAAAAAWARRRGGQAVQPDEPPGPVARADRALMSAIRGTVRRHMWDDVGIVRTASSIQ
                     RALEGLGREAWLDAVVFRSREALEVRNLRLVAELVAKAALQRRGSLGCHFRRDFPSKG
                     TGWRRHISVVL"
     gene            complement(578..1258)
                     /locus_tag="AC16BC_05255"
     CDS             complement(578..1258)
                     /locus_tag="AC16BC_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3241060.1"
                     /translation="MRKLRAIVTVLIAGLAGLASSAQADSQIFRYVAPDGSIHYANAP
                     MTGSYRPYSIRADVGFSKLRPGKPNRSRFYSIIDKTARDHQMDPALIRAVVKAESDYD
                     PRAVSSAGALGLMQLMPGTAQDLDVNNPFDPEENVRGGVQYLRYLLTRFNGNTSLALA
                     AYHAGEQNVERHNGVPPIEATQTYVKRVLNFHKKYRQTMPPPEPAIYRVRSGDTIHYT
                     TTPPAEYR"
     gene            1430..1870
                     /locus_tag="AC16BC_05260"
     CDS             1430..1870
                     /locus_tag="AC16BC_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3241061.1"
                     /translation="MRWEPFKDLLSLQDRMNQLFEESLARGAAKQASERPAHWSPSVD
                     ILETETELVLLAEVPGVDLEDIELQIHDNVLTFRGERMFDNSVKKEHYFRIERAYGNF
                     SRSFTLPSSVDSERVEARLKDGILEVRMPKRTQGTSRSITVEVK"
     gene            1875..2261
                     /locus_tag="AC16BC_05265"
     CDS             1875..2261
                     /locus_tag="AC16BC_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011049905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein transcriptional repressor
                     HspR"
                     /protein_id="HIR3241062.1"
                     /translation="MADDSTRPLYHIGGVAALAAVHPQTLRIYEREGLLKPSRSRGKT
                     RLYSERDVERVKLIVHLTRDEGLNLAGVAKILELQDQIREVEVVIQRWMHEWHERMVR
                     ELATRNERVDAAPRKGRAIAVPIRRG"
     gene            2523..5123
                     /gene="clpB"
                     /locus_tag="AC16BC_05270"
     CDS             2523..5123
                     /gene="clpB"
                     /locus_tag="AC16BC_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR3241063.1"
                     /translation="MRPDRFTVKTQEALQEAQRSAEKRGHQQLDAEHLALALATQDDG
                     VVPPLLKKIGVDVGRLRQDLERALDRVPKVTGGGGQLYAAPRLQETLNKAEQEAERLK
                     DEYVSTEHLLIALVDVGGEIATLLKAAGVTKDRLLAALTEIRGSQRVTDQNPEDKYQA
                     LTRYSRDLTDLARKSKLDPVIGRDEEIRRVIQVLSRRSKNNPVLIGEPGVGKTAIAEG
                     LAQRIAHGDVPEGLKNKRVMSLDMGALIAGAKYRGEFEDRLKAVLKEVSAAEGEVILF
                     IDELHTLVGAGAAEGAMDASNMLKPALARGELRCIGATTLDEYRKHIEKDAALERRFQ
                     PVMVAEPSVESTISILRGLKGRYELHHGVRIRDAALVSAAVLSHRYITDRFLPDKAID
                     LIDEAASRLRIEIDSMPTELDEVERKIRQLEIEREAVKKEHDDASRERLERIEKDLAE
                     LGEEKKRLTAQWQTEKGSIGSLRALKADIEKTIEDAERAERAGDLGRAAELRYGRLID
                     LNKKLEAEQLRLKDVHSTTRLLKEEVDEEDIAEIVGKWTGIPVTRLLEGEIQKLVHME
                     ERLRERVVGQEEAVLAVSNAIRRARSGIADPNRPMGSFIFLGPTGVGKTELAKTLAQF
                     LFDQEQALIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGQLTEAVRRRPYAVLLFDEI
                     EKAHADVFNILLQLLDDGRLTDGQGRTVDFKNTVVIMTSNAGSQFIQELGHDETAMRN
                     QVMAALREQFRPEFLNRVDDIIIFHPLRADQIGKIVDFQLAAVAKRLEERHVKVELTD
                     AAKAVLAVEGYDPVYGARPLKRVIQRDVLNPLSLKLLSGEFRDGDVIRVDVHEGGLKF
                     EKAGVAQAAS"
     gene            5584..6168
                     /locus_tag="AC16BC_05275"
     CDS             5584..6168
                     /locus_tag="AC16BC_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241064.1"
                     /translation="MEISVPVDRLSMGESEMEREVLPLLVNQVFHVTSYERYLKIAES
                     GHISHNADDRHGHIYGQSLNSIGRKLKAVCLFDLRNKDLTSIEFELGCYHLPTVPQFG
                     RTVTFLFLKTSEYARLVQWDSLDSNDKMSGQHIPKIESWYPGDLVVSKLSKVLIVDVD
                     RPVRKGSAELLADLIERTGWQPGPQENNEEPGDL"
BASE COUNT         1412 a         1957 c         2255 g         1330 t
ORIGIN      
        1 agaccgacgt gcgagggacc acgagcgtgc cgggcctgtt tgcggtgggc gaggccgcgt
       61 gcaccggcgt gcatggcgcg aaccgcttgg ccagcaattc gctgctcgaa ggactggtgt
      121 tcggcacgcg agccggcgcg gccgccgcgg cctgggcgcg tcggcgcggc gggcaagcgg
      181 ttcaaccgga tgaaccgccc gggcccgtgg cccgagccga tcgggcgttg atgagcgcga
      241 tccggggaac cgtgcgtcgg cacatgtggg acgacgtcgg catcgtgcgg accgcctcgt
      301 cgatccagcg tgcgctcgaa gggttggggc gagaggcgtg gctggacgcg gtggtgttcc
      361 gctcacgcga ggcgctggaa gtcaggaatc tccggctggt ggcggaactg gtcgccaagg
      421 cggcgttgca acgccgggga agtctcgggt gtcacttccg aagggatttt ccgtccaagg
      481 gcacgggctg gcgccgtcac atctcggtgg ttctgtaacg agtcgcttcc atgcggatcg
      541 cgacgcaacg cagtagatgg acgttttcag ccgcctgtca gcggtattcg gccggcggcg
      601 tggtggtgta atggatcgtg tcgcctgacc gcacgcggta gatcgcgggt tccggcgggg
      661 gcatggtctg ccggtacttt ttgtgaaagt tcaacacgcg tttcacgtac gtctgagtcg
      721 cttcgatcgg ggggacgccg ttgtggcgct ccacgttctg ctcgcccgcg tggtacgcgg
      781 ccaacgcgag cgacgtgttt ccgttgaacc tggtcaacag ataccgcagg tactgaaccc
      841 cgccccgcac gttttcctcc ggatcgaacg ggttgttgac gtccagatct tgcgcggtgc
      901 ccggcatgag ctgcatcaat ccgagcgcgc cggctgacga cacggcccgc gggtcataat
      961 ccgattccgc tttgaccacg gcccggatca acgcggggtc catctggtgg tcgcgggcgg
     1021 tcttgtcgat gatcgagtaa aagcggcttc ggttcggttt tcccggtctc agcttggaaa
     1081 acccgacatc ggcgcggatg gagtacggac ggtaactgcc ggtcatcggg gcgttggcgt
     1141 agtggatcga accgtccggc gcaacatagc ggaaaatctg tgaatcagcc tgtgcggagg
     1201 atgccagacc ggccaggccc gcgatcagga ccgtgacaat ggcgcgaagt ttccgcatga
     1261 cggttgaaaa cgctaccata gggagtggac agtcgtcaaa agctggggat tccttcaaag
     1321 gtcattactt gacaataata ttatcaaatt atataaatat gcaggaatag acttatcgtt
     1381 tagtcgaagg atagccgcca gtattgccat tatctattga ggaggggtta tgcgctggga
     1441 gccatttaaa gacttgctgt cattacagga ccggatgaac cagctctttg aagagtcctt
     1501 ggcgcgcggc gcggccaagc aggcttccga gcgcccggcg cattggagcc cgtcggtcga
     1561 tatcctggag acggaaaccg aactggtcct gctcgcggag gtcccggggg tggatctgga
     1621 ggacattgaa ctgcagatcc acgacaacgt gctgacgttt cggggtgaac ggatgttcga
     1681 caacagcgtg aagaaagagc actacttccg gatcgagcgc gcatacggca atttcagccg
     1741 gtccttcacg ctgccgtcct cggtggattc ggagcgcgtc gaggcccggc tcaaggacgg
     1801 catcttggaa gtgcgcatgc ccaaacgcac gcaggggacg agccgctcca tcacggtgga
     1861 ggtcaagtaa cgcgatggcg gatgactcga cgcgtccgct ctaccacatc gggggggtgg
     1921 cggcgctcgc ggccgtgcat ccgcagacgt tgcggatcta cgaacgcgag gggctgctca
     1981 agccgtcgcg gagtcgcggc aagacgcgct tgtactcgga acgcgacgtc gagcgggtca
     2041 agttgatcgt gcacctcacg cgcgacgagg gactgaatct cgcgggcgtg gccaagatct
     2101 tggagttgca ggaccagatt cgggaagtcg aggtggtgat tcagcggtgg atgcacgaat
     2161 ggcatgagcg gatggtgcgc gagctggcga cccgcaacga gcgcgtggac gcggcgccgc
     2221 ggaaagggcg cgccatcgcg gtgcccattc gccggggata ggcgcctgtc caggaaaggc
     2281 catctgcggc gttgtcggtc ggccttgcgt gctcacgtac gacccagtac gctccgcgcg
     2341 caaggcctcc cttgcttcgc cacccgagag gaccacgccg tttgtatttt gcgccgttgg
     2401 cgcaaagcgg gttcgcctgg catctggcct tccctgaaca ggcgccaacg tggcgaactc
     2461 ggactggtgc cgaggtcggt ggtgcgcagt attagtgttg atcgagagga ggatgtgtcg
     2521 cgatgagacc tgatcgattc acggtcaaaa cccaagaggc gttgcaggaa gcccagcgga
     2581 gcgcggaaaa acgcgggcac cagcagcttg acgccgaaca cctggccttg gcgctggcca
     2641 cccaggacga cggggtggtc ccgccgttgc tcaagaaaat cggcgtggac gtggggcggc
     2701 tgcggcagga tctcgaacgc gcgctggatc gtgtgcccaa ggtcacgggc ggcggggggc
     2761 aactgtacgc cgcgccccgg ctccaggaga ccttgaacaa ggcggagcag gaagccgagc
     2821 ggctcaagga cgaatacgtc agcaccgagc acctgttgat cgcattggtg gacgtcggcg
     2881 gcgagatcgc gacgttgctc aaggccgcgg gcgtgaccaa ggatcgcctg ctggcggcgt
     2941 tgaccgaaat ccgcggcagc caacgggtga cggaccagaa tcccgaagac aagtatcagg
     3001 cgctgacccg gtacagccgg gatctgaccg atctcgcgcg caagagcaag ctcgatccgg
     3061 tcatcggccg cgacgaggaa atccgccgcg tgatccaggt cctctcgcgc cggagcaaga
     3121 acaacccggt gctgatcggc gagccgggcg tgggcaagac cgcgatcgcg gaaggcctgg
     3181 cgcagcggat cgcgcacggg gacgtgcccg aggggctcaa aaacaaacgc gtgatgagcc
     3241 tggacatggg cgcgctgatc gcgggggcca agtatcgcgg cgagttcgag gaccgactca
     3301 aggccgtgct caaagaggtt tcggccgctg aaggagaagt tatcctcttc atcgacgagt
     3361 tgcacacctt ggtgggcgcg ggcgcggccg aaggcgccat ggacgcttcc aacatgctca
     3421 agcccgcgct cgcgcgcggc gagctgcgct gcatcggcgc caccacgctt gatgaatacc
     3481 gaaaacacat tgaaaaggac gcggccttgg agcgccgctt ccagccggtc atggtcgcgg
     3541 aacccagcgt ggagtccacg atctcgattc tccggggctt gaagggccgg tacgaactgc
     3601 accatggggt gcgcatccgc gacgcggcct tggtgtccgc ggccgtgttg tcgcaccgct
     3661 acatcacgga tcgattcctg ccggacaagg ccatcgactt gatcgacgag gcggcctcgc
     3721 ggcttcggat cgaaatcgac agcatgccga ccgagttgga cgaggtggaa cgcaagatcc
     3781 ggcagttgga aatcgagcgc gaagccgtga agaaagagca cgacgacgcc tcacgcgaac
     3841 gtttggaacg gatcgaaaaa gacttggcgg agttgggcga ggagaagaag cggctgaccg
     3901 cccagtggca gacggagaag ggctcgatcg gatcgctgcg cgcgttgaag gcggacatcg
     3961 aaaagacgat cgaggacgcg gagcgcgcgg aacgcgcggg cgacctgggc cgagccgcgg
     4021 aattgcgata cggccggttg atcgacctca acaagaaact cgaagccgag caacttcgcc
     4081 tcaaggacgt gcactccacc acgcgcctgc tcaaagagga agtggacgaa gaggacatcg
     4141 ccgagatcgt gggcaagtgg accgggattc cggtcacccg gttgttggaa ggcgaaatcc
     4201 agaagctcgt gcacatggaa gagcgcttgc gggagcgcgt ggtcgggcag gaagaagccg
     4261 tgttggccgt gtccaacgcg atccgccgcg cccggtcagg gatcgcggac cccaatcgtc
     4321 ccatgggttc gttcatcttc ctggggccca cgggcgtggg caagaccgag ctggccaaga
     4381 ccttggccca gttcctgttt gatcaggagc aggcgttgat ccggatcgac atgtccgagt
     4441 acatggaaaa acacgcggtc gcgcgcttga tcggcgcgcc tccgggctac gtgggctacg
     4501 aagaaggcgg tcagctcacc gaggccgtgc ggcggcgacc gtacgcggtg ctgctcttcg
     4561 acgaaatcga aaaggcgcat gctgacgtgt tcaacatcct gctccaatta ctggacgacg
     4621 gacggctcac ggacggccag ggccggaccg tggacttcaa gaacacggtg gtgatcatga
     4681 cctcgaacgc gggcagccag ttcattcagg aactggggca cgacgaaacc gcgatgcgca
     4741 accaagtcat ggccgcgttg cgcgagcaat tccggcccga gttcctcaac cgcgtggacg
     4801 acatcatcat cttccacccg ttgcgggcgg accagatcgg caagatcgtg gacttccaac
     4861 tcgcggccgt ggccaagcgg ctggaagagc ggcacgtgaa ggtggaactg accgacgcgg
     4921 ccaaggccgt gctggccgtg gaaggctacg acccggtcta cggcgcgcga ccgctcaaac
     4981 gagtgatcca gcgcgacgtg ctgaacccgc tgtccctgaa gctcctgtca ggcgagttcc
     5041 gcgacggcga cgtgatccgc gtggacgttc acgaaggcgg cttgaaattc gaaaaagccg
     5101 gagtcgcgca agccgcgtcg taataagagg ggggcgcgtc tcgcgccccc ccgatttacg
     5161 tcttcaagtt ttttactgat cagtttttct tatccccccc tttggaaaag ggggggcgag
     5221 gggggatttg attttctgcc ccgcccgcat cctctcaagc cctgcgtcgc ttgattgccg
     5281 ccctcaaacc agttattctg ccgacacgca atccagcttt gtctagaagc gcggcgcgtc
     5341 cctgcgcgtc gaatcaagct ccagggttgt ccggagccca gcgtcggctc agcctggaat
     5401 gcgtcgtcgc gacgaagcgt cggagccaca gaccatagga agatggggac caaaacccta
     5461 tctcgatatc agatacctgc gctgtagatt ttcgcgcgat tttccggttg ggtcgacggg
     5521 gtcaaaaaca aatcaattcg tccatctact aacacatcga ctaatttgca gcaggactga
     5581 cttgtggaaa tttccgttcc agtagatcgg ctttcaatgg gtgagagcga aatggaacgc
     5641 gaggtgttgc cgctgcttgt aaatcaggta ttccacgtca cgtcctatga gcgatacttg
     5701 aagattgccg agtcaggtca catttcgcac aatgctgacg atcgccatgg acacatctac
     5761 ggccagtccc tgaactccat tggccgaaaa ctaaaggcgg tgtgcctatt cgaccttcgg
     5821 aacaaggact taacgtcgat cgagtttgaa ctaggctgtt accaccttcc aacagtacct
     5881 cagtttggca ggacggtgac tttccttttc ttaaaaactt ctgagtacgc tcgactagtg
     5941 cagtgggata gtctggacag taacgacaaa atgtctgggc aacacatccc aaagattgag
     6001 agttggtacc ccggagatct tgtcgtctcc aagctcagta aggtcctaat cgtcgatgtc
     6061 gatcgcccgg tccgtaaggg ctcggccgaa ttgcttgccg acttgatcga gcggaccggt
     6121 tggcaaccgg gaccgcagga aaacaatgag gagcccggcg atttataggg tattgagggc
     6181 ttttgagccc agaaaacctg gactcgaagc catgccattt acagttgatt ttgaccccgt
     6241 tgacggaata ccgtgagcac tgcgttgcgg ggcgtctggt caacgcgtta tgcgtggggt
     6301 ttttgatcac ggcgtgttag gacgcaaatt atgcgtccta acacgcttca atcaccaaaa
     6361 gaggtgtaag tcgtaaccct gtgaaaaagt agggtggtcg gcgaatttag cggccaaatt
     6421 gtcttgacgg ttatgaagca atcaacgcct ggtttgtcga aataattagg gggcctaatt
     6481 ttagttaggt tgcctatgac caacacaccc ttgtgcaaga tgatcgcatc tatattgagc
     6541 ctcctgctct ttgccgtgct ctacatgctg gccggtctgc tcaatcttgg gccaaaaagc
     6601 ttcgtgtacg cgcttactta cggtgtagtt ctacttgggt gccctatcgt gttatttctt
     6661 cttgttagcg gggcagagaa acgcctggta ctacaaagta tccggattgt cttttcgttg
     6721 ctcgtgggtt ttctgagcca actaccgctt ggtgctcttt ttgacacaat gaattggcca
     6781 atttttcata cttgggggtt gggccatgga tcatttctta ttgccgtacc tgccatcggc
     6841 agcgtcccat accttttgct gggccgcgct ttccggctca ccaatcaaca gaatgcaacc
     6901 taacccacgc ttggaggcgg actcgctacg tcggcgcttc gcgccgcctc cgct
//
LOCUS       DBNGTT010000237         4156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2222181, whole genome shotgun sequence.
ACCESSION   DBNGTT010000237 DBNGTT010000000
VERSION     DBNGTT010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2222181"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2117)
                     /locus_tag="AC16BC_05280"
     CDS             complement(<1..2117)
                     /locus_tag="AC16BC_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3241065.1"
                     /translation="MRRAIEFVVRRPWWVLVATAALTIVFAVQMLKVRMVIDPKTILP
                     QQHPYVQLNNVIEEVFGGSRVVVVGVVPKHGDIFTPQHLATLKALTDEVKKIPGIKEE
                     NVVSLADRKVKFVTADGESIKVERLMTEVPTTPDEAEAFRRRVMSNDLYVNGLVSADG
                     RAAAIITDFREWVPTWKENQQQLSTAPQDWPKNPDEWASESWFGGSRGAIHTAYGFRM
                     GDEQIRMALQEAAARVAAEDVDIHFGGLPIALSYLEADSKRIVYLFPISLVVMMVVLY
                     WAFRSLQGMALPLATALISVVWALGLMGTFRIPMDPFNTMTPILIMAIAAGHAIQILK
                     RYYEELAAGLDNRRAVIEATAKMAPVMITAGLVAAASFASLVTFHLKTFRAFGMLTAF
                     GIISALVLELTFIPALRSLLRPPAVAVSVRDGLDRLLARLAAVVTGNRRTWVAVGALV
                     VAVVGAAGASRVVVNNSLKNQFFESTRLRIDERALNTHFGGTSTFYVMVEGQQQDALK
                     RADVVAAIADLQQRIAAVPGVGKTESFVDYLRKMNRTFHGDQPEFDRIPANDGEVSNF
                     LFLYSISGNPADFSRLIDYRYRRAVIWSFLKTDSTALAEQLIAVVDAQRAVFERLGVT
                     VGVAGSSPVTVALNRTMVDGKLQNILQIGAIIFVASAVVLRSLVGACLVLVPLGLAVL
                     VNFAVMGFGGVTLGIGTAAISAMA"
     gene            complement(2218..3027)
                     /locus_tag="AC16BC_05285"
     CDS             complement(2218..3027)
                     /locus_tag="AC16BC_05285"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR3241066.1"
                     /translation="MKRFAIGVAVVGLLVWAAGISGAAEGELSGLDVMKKTRELYLAK
                     DQISKVTLRKIDKRGDEQKIETIRYWKAYGGKDGFESKTLFATTFPPDQRGVGFLIWD
                     HSKAGQPDDLRLYLPSLKQIRRMTTREQDDAFMGSDLTFADLGQRELAEDEHTLLRTE
                     KCGGAARDCYVVQSVPKDKESLYGKKITWVPTVDFTPVRVDYYDRKGDLLKTQAIEWQ
                     HPDNVWAWKRSEVKNVQTEHRTLFEISDLKNNVGLTDDDFSERAVKAGPRR"
     gene            complement(3050..4114)
                     /locus_tag="AC16BC_05290"
     CDS             complement(3050..4114)
                     /locus_tag="AC16BC_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR3241067.1"
                     /translation="MAKAKATKGKSVRRQGSARKRPTAKALDPMTVARISSAYWHSKV
                     LHVANRLDVFNRLAGKTVSSEDLAREIGADPRGLEIVLIAATSLGLLQRVKDRYRNSP
                     LADTFLVKTSPRYQGGIVSMFEDWYPTWGKLYDAVMTGKPPVQKPHDQGEEATRTYIY
                     GMHYRGVAQAQLLAKKISLKGRSHLVDIAGGPGTFPIMFCKANKGLSATVLDRPQTLA
                     VTREIVASYQMTGRVETKPADYLSDAFGQGYDAALLSSMLNQESPAVIQEIFRKAYQA
                     LTPGGLVIVQDQMLNDEKSGPVLSAMIGVNQLLHTPGGAAHSSREVAEWLAAAGFGKL
                     KRVPMPASSPFTVLTGIKPS"
BASE COUNT          753 a         1402 c         1230 g          771 t
ORIGIN      
        1 gccatggccg agatcgccgc ggtgccgatc ccgagcgtga cgccgccgaa gcccatcacc
       61 gcgaagttga ccaagaccgc caagcccaac ggcaccaaca ccaggcaggc cccgaccagc
      121 gatcgcagga ccacggccga cgcgacgaag ataatcgcgc cgatctggag gatgttctgg
      181 agcttgccgt ccaccatcgt gcggttgagc gccaccgtga ccggcgaact gcccgccacc
      241 ccgaccgtca cccccagccg ctcgaacacc gcgcgttgcg catccaccac ggcgatgagc
      301 tgttcggcga gggccgtgga atcggtcttg agaaaactcc agatcacggc gcggcggtag
      361 cgataatcga tcaaccgcga gaaatccgcc ggattgccag aaatcgagta caggaacaag
      421 aaattggaca cctccccgtc gttcgccgga atacggtcga actcgggttg atccccgtga
      481 aacgtccgat tcatcttccg cagataatcg acgaacgatt cggttttccc aacgcccggc
      541 acggccgcga tgcgctgctg caggtccgcg atcgcggcca cgacatcggc ccgtttgagc
      601 gcgtcctgct gctgcccctc aaccatcacg taaaaggtcg atgtgcctcc gaagtgcgta
      661 ttgagcgcgc gttcgtcgat ccgcaacctg gtggactcga agaactggtt cttcaagctg
      721 ttgttgacca cgacccgaga cgcgcccgcc gcgcccacca cggccaccac gagcgcgccg
      781 accgccaccc acgttcgccg gttcccggtg accaccgccg ccaatcgagc caacagccgg
      841 tccaacccgt ctcgaaccga cacggccacg gccggggggc gcagtaacga gcgaagcgcc
      901 gggataaagg tcagttccaa caccagggcg ctgatgatcc cgaacgcggt caacatgccg
      961 aacgcgcgga acgtcttgag gtggaacgtg accagcgagg caaagcttgc ggcggccacc
     1021 agccccgcgg tgatcatgac cggcgccatc ttcgcggtag cctcgatgac cgccctccgg
     1081 ttgtccaatc cggcggccag ctcctcgtaa taccgtttga gaatctggat cgcgtgcccc
     1141 gcggcgatcg ccatgatcaa gatcggcgtc atggtgttga acggatccat cgggatccgg
     1201 aacgtcccca tgagtccgag cgcccacacg accgagatca gcgcggtggc caacggcagc
     1261 gccatgccct gcaaactgcg gaaggcccag tacaggacca ccatcatgac caccaacgag
     1321 atcgggaaca gatagacgat ccgcttggag tcggcctcca ggtacgacag cgcgatgggc
     1381 agtccgccga agtggatgtc cacgtcctcc gcggccacac gcgcggcggc ttcttggagc
     1441 gccatccgaa tctgctcgtc ccccatccgg aagccgtacg cggtatggat ggcgccgcga
     1501 ctgcctccga accaggattc gctcgcccac tcatccgggt ttttcggcca atcctggggc
     1561 gcggtgctca gttgctgttg gttctctttc cacgtgggca cccattcgcg gaagtccgtg
     1621 atgatcgcgg cggcgcgccc gtcggcggac accagcccgt tgacgtacag atcgttcgac
     1681 atgacgcgac gccggaacgc ctccgcctcg tcgggcgtgg tcggcacctc ggtcatgagg
     1741 cgttcgacct tgatcgattc cccatcggcg gtcacgaact tgaccttccg atccgccagg
     1801 ctcacgacgt tctcttcctt gatcccgggg atctttttga cttcgtcggt gagggccttg
     1861 agcgtggcca agtgctgcgg cgtaaagatg tcgccgtgtt tgggcaccac gcccaccacc
     1921 acgacgcggc tcccgccgaa gacctcctcg atcacattat tgagctgaac gtacgggtgt
     1981 tgttggggca ggatggtctt ggggtcgatg accatccgga ccttcaacat ctggacggca
     2041 aacacgatgg tgagggccgc cgtggcgacc agcacccacc acggccggcg gacgacgaat
     2101 tcgatcgctc gtctcataga tccctaccac cagaccgtgg gaagcgcccc ggcattcccc
     2161 gggacaacct cggacgtcca cgaacggtcc gtgcgcgatg ggctgggggt ggcttgctta
     2221 gcggcggggg ccggccttca cggctcgctc gctgaaatcg tcgtcggtga ggccgacatt
     2281 gttcttgagg tcgctgatct cgaacagcgt ccggtgctcg gtctggacgt ttttgacctc
     2341 agaccgtttc cacgcccata cgttgtcggg gtgctgccac tcgatcgcct gggtcttgag
     2401 caaatcgccc ttgcggtcat agtaatcgac gcgaaccggc gtaaaatcca cggtcggcac
     2461 ccaggtgatc ttcttcccgt agaggctctc tttatccttg ggcacgcttt ggaccacgta
     2521 acagtctcga gccgcgccgc cgcacttctc tgtccgcagc aaggtgtgtt catcctccgc
     2581 caactcacgc tggccgaggt cggcgaacgt caggtcggac cccatgaagg cgtcgtcctg
     2641 ttcgcgggtc gtcatgcggc gaatctgttt caggctcggc agatagaggc gtaagtcatc
     2701 gggctgaccg gctttgctgt ggtcccagat caaaaacccg acgcctcgtt gatccggcgg
     2761 aaaggtggtg gcaaacagcg ttttgctttc gaatccgtcc ttcccgccgt aggccttcca
     2821 ataccggatg gtttcgatct tctgctcgtc gcctcgcttg tcgatcttgc ggagggtaac
     2881 cttcgaaatt tgatccttgg ccaggtagag ctcccgggtt ttcttcatca cgtcgagccc
     2941 cgaaagttcg ccctccgccg cgccggagat ccccgcggcc caaaccagca gcccaaccac
     3001 cgccacccct atcgcaaatc gcttcatcgc cgttctccct ccgccccgat caggagggct
     3061 tgatcccggt gagcaccgta aagggactcg aggccggcat cggaacccgt ttgagcttgc
     3121 caaacccggc tgcagccaac cactcggcga cttcccgaga cgagtgggcg gctccgccgg
     3181 gcgtatggag caactgattc accccgatca tcgcggacag cacgggtccg gacttctcgt
     3241 cgttgagcat ttgatcctgg acgatcacga ggcccccggg cgtcagcgcc tgataggcct
     3301 ttcggaaaat ctcttggatc acggcgggcg attcctgatt gagcatcgac gacagcaacg
     3361 ccgcgtcata cccctgcccg aaggcgtcgc tcagatagtc cgcgggtttg gtctcgaccc
     3421 ggccggtcat ctggtaactc gccacgatct cacgcgtcac ggccagggtc tgcggacgat
     3481 cgagcacggt cgccgacaat cccttgttcg ccttgcagaa catgatggga aacgtaccgg
     3541 gcccgcccgc gatgtccacc aggtgcgacc ggcccttgag agagatcttt ttcgccaaca
     3601 gctgcgcctg cgcgacgccg cgatagtgca tcccgtagat gtacgtgcgg gtcgcctctt
     3661 cgccctgatc gtgcggtttc tgcaccgggg gtttgccggt catgaccgcg tcatacagct
     3721 tgccccaggt gggataccaa tcttcgaaca tcgacacgat cccgccctga tatctcgggc
     3781 tggtcttgac caagaacgtg tcggccagcg ggctgttgcg gtaccggtcc ttcacgcgct
     3841 gcaacaggcc gagcgacgta gcggcgatca acacgatctc caaaccgcgc gggtccgcgc
     3901 cgatctcacg cgcgagatcc tcggaggaca ccgttttgcc ggccaaccgg ttgaacacat
     3961 ccagccgatt cgcgacgtgc aggaccttgg aatgccagta cgcggaactg atgcgcgcga
     4021 ccgtcatggg gtcgagcgcc ttcgcggtcg ggcgcttgcg cgccgatccc tgccgacgta
     4081 ccgatttgcc ctttgtcgcc ttcgccttcg ccatcgcgtc tcctcaatgc gggtggttcg
     4141 ggcgccgtct gcgccc
//
LOCUS       DBNGTT010000238         4019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_51295, whole genome shotgun sequence.
ACCESSION   DBNGTT010000238 DBNGTT010000000
VERSION     DBNGTT010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_51295"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16BC_05295"
     CDS             complement(<1..235)
                     /locus_tag="AC16BC_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241068.1"
                     /translation="MSTEPATAIIVKGARAHNLKNLDVTIPRNRLVVITGLSGSGKSS
                     LAFDTIFAEGQRRYVESLSAYARQFLQQMDKPDV"
     gene            complement(295..2670)
                     /locus_tag="AC16BC_05300"
     CDS             complement(295..2670)
                     /locus_tag="AC16BC_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR3241069.1"
                     /translation="MIPLNSRRGELERRTETLLDWPVLLARLAEHAVTPIAKERCVAL
                     EGTADPATVSGRYEELQEWTTLRDRAGDLAFAGGADPRAALRAAVPGGLLDAEQCREI
                     AVVLGQVRDVRRALVPSPDLARLAARAQALDEVPEVRRELDEAIDAQGRLRESATPEL
                     RRLRREVDRLRQTLIDRVGELLSSPRYEPWLQDTFVTQRQDRYVLPIKIEHRAKVAGI
                     IHELSASGATVFIEPEELVEANNDLKWAEMAVAQEIERIFRRLTDLIAAQRNALETNV
                     EILTALDVIQAKGRLGLAYHGMIPTISRTREIAWRGLRHPLLALRGIAVVPNDVTLEP
                     DARALVISGPNTGGKTVLLKAMGLAALMTRAGIPLPCDPDSVSPVFPAVLMDAGDDQD
                     LARDLSTFAAHVRVVCALIDTAPPGSLILLDELATATDPEEGAALAQALVEAFMARNV
                     LTVVTTHYTALKAWAAQEAGARDRVSAGMGYDVETMTPTYRLALGRPGASLGIDVAAR
                     LGLPTSILARARGLVSPQAAALGDAIAALERERRVATDLRQRLTALEAAARDAALRQD
                     AAAAQLEREREAFAATKRERLAAEVRRAKTEMDAILEQARREPDARKIRVLIPKIAAT
                     AESRHETPSEPESVPPRDFDAGARVRVASLRADGVLVDATSGRKRVRVLVGDREVSVS
                     PGDLRPAKPAEPSGISGTVGRPVAPEPAPDTVNVIGQRVDAALERVDRALDKAAAAGA
                     TRLRVLHGHGNGRLKRAIREHLAGSPYVASHRPGDDTEGGDAATVITLTTG"
     gene            2786..3196
                     /locus_tag="AC16BC_05305"
     CDS             2786..3196
                     /locus_tag="AC16BC_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3241070.1"
                     /translation="MQSHDVTVLLSEKALKVTPQRVAVMEFLRGNTTHPSVDDVYQQV
                     RKRFPSISRATIYNTVTTLVEAGLLQEILIQQEKTHVDWNVDRHHHFQCLKCGTVKDI
                     RYDAVTAEQVSKHVVGHLVTGVKVVMEGVCESCR"
     gene            3608..>4019
                     /locus_tag="AC16BC_05310"
     CDS             3608..>4019
                     /locus_tag="AC16BC_05310"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3241071.1"
                     /translation="MCAAAVALGAVVPGVARAQATDLVKEANNFFKPLPDAMPAPKDN
                     PTTTVRATLGKMLYFEPRLSKTNTISCNSCHNLATGGVDNLPTSMGHLAQFGPRNSPT
                     VLNAGLQISQFWDGRAASLEDQAKGPILNPLEMAM"
BASE COUNT          681 a         1248 c         1368 g          722 t
ORIGIN      
        1 ccacatcggg cttgtccatc tgttgcagga actgccgggc atacgcggac aacgattcca
       61 catagcgccg ctgcccttcg gcaaaaatcg tgtcgaacgc cagcgacgac ttcccggagc
      121 ccgacaaccc cgtgatcacg acgagccggt tgcgcgggat ggtgacgtcg aggttcttga
      181 gattgtgcgc tcgcgcccct ttgacgatga tggcggtggc gggttcagtg ctcacgggtc
      241 gtgtcgacgt cggcgaaaaa ttgcatggta acacacggcg ggcgttggcc gcgatcagcc
      301 ggtggtcagg gtgatgacgg tcgcggcgtc gccgccctcg gtgtcgtcgc ccggacgatg
      361 ggacgccacg tagggggagc cggccaggtg ctcccgaatc gcgcgtttga gccggccgtt
      421 gccgtggccg tgcagcacgc ggaggcgcgt ggcgcccgcg gcggccgcct tatccaatgc
      481 gcgatcgacg cgttccaacg cggcgtcgac gcgttgcccg atcacgttga cagtgtcggg
      541 agccggttcc ggagcaacgg gacggccgac cgtcccggag atgcccgacg gctccgcggg
      601 cttggcgggc cggaggtcgc cgggggaaac cgagacttcg cgatcgccca ccagcacccg
      661 cacgcgtttt cgtccggacg tggcgtccac gagcacgccg tccgcgcgca acgacgccac
      721 ccgcacgcgc gctcccgcgt caaaatctct cggcggaacc gactccggct ccgagggcgt
      781 ctcgtggcgg gattcggccg tggccgcgat tttcggaatc aacacgcgga tcttccgcgc
      841 atcgggttcg cgccgcgcct gctccaggat tgcgtccatc tccgttttgg cgcggcggac
      901 ttcagcggcc aggcgttcgc gtttcgtggc tgcgaaggcc tcccgctcgc gctccaattg
      961 cgccgcggcc gcgtcctgac gcaatgcggc gtcccgcgcc gcggcttcca gcgcggtcag
     1021 gcgctggcga aggtcggttg ccacacggcg ctcgcgctcc agcgccgcga tcgcgtctcc
     1081 cagcgcggcg gcttgcgggg acaccaatcc gcgggcccgc gccaggatcg aggtcggaag
     1141 ccccaggcgg gccgccacgt cgatgcccag actcgcgccg gggcgtccga gcgcgagccg
     1201 gtacgtgggc gtcatggtct ccacgtcgta ccccatgccc gcgctgacgc ggtcgcgcgc
     1261 gccggcctcc tgcgcggccc acgccttgag cgcggtgtag tgcgtggtca ccacggtcaa
     1321 cacgttcctg gccatgaacg cttcgaccag ggcttgcgcc agggccgcgc cctcttccgg
     1381 gtcggtggcg gtggccagtt cgtcgagcag gatcagcgag ccggggggcg cggtgtcgat
     1441 caacgcgcat accacgcgca cgtgcgccgc gaacgtggac aggtctcgcg ccagatcctg
     1501 atcgtcgccc gcgtccatga gcacggcggg gaataccgga gagactgaat cagggtcaca
     1561 cggcaggggg atcccggcgc gggtcatgag cgcggccagg cccatggcct tgagcagcac
     1621 ggttttcccg ccggtgttgg gcccggagat gacgagggcg cgggcgtcgg gctcgagcgt
     1681 cacatcgttg ggcacgaccg cgatgccgcg cagcgcgagc aatggatgcc gcagcccgcg
     1741 ccaggcgatc tcccgggttc gggagatggt cggaatcatg ccgtgatacg ccaggcccag
     1801 gcggcctttg gcctggatca cgtccagcgc ggtgaggatc tcgacgttgg tttcaagggc
     1861 gttgcgttgc gcggcgatga gatccgtgag ccggcggaag atgcgctcga tctcctgcgc
     1921 caccgccatc tcggcccatt tgaggtcgtt gttggcctcg acgagttctt cgggttcgat
     1981 gaacacggtg gcgccgctgg ccgacaattc gtggatgatg cccgcgactt tggcgcggtg
     2041 ttcgatcttg atcgggagca cgtagcggtc ttggcgctgc gtgacgaatg tatcctggag
     2101 ccagggttcg taacgcggcg aactcagcaa ctcgcccacg cgatcgatga gggtctggcg
     2161 caggcggtcc acctcgcggc gcagacggcg aagttcgggc gtggcggatt cgcgcagccg
     2221 gccttgcgcg tcgatcgcct cgtccagttc gcgacgaacc tcgggcactt cgtccagcgc
     2281 ctgcgcgcgg gcggccaagc gggcaagatc ggggctcggg accagcgccc gtcgcacgtc
     2341 gcggacttgg cccagcacca ccgcgatctc gcggcactgc tcggcgtcga gcaaccctcc
     2401 gggcacggcc gcgcgcaacg cggctcgcgg gtcagcccct cccgcgaacg cgagatcgcc
     2461 ggcgcgatct cgaagagtcg tccattcctg gagttcctca tatcggccgg acaccgtcgc
     2521 ggggtcggcg gttccctcga gcgccacgca tcgttccttg gcgatcgggg tgacggcgtg
     2581 ttccgcaagc cgggccagga gcacgggcca atccagaagc gtttcggtcc ggcgttcgag
     2641 ttcgccccgg cgtgaattga gggggatcat cgtgacccgc ggagcataac aaggtccgga
     2701 actcctcagc aagcaacaat cttgttctca aaaaataata aatcctgcta ttgacaaatt
     2761 cctaggttag atttattatt ctggcatgca atcgcatgat gtcacggttc tcctgagcga
     2821 aaaggcgctg aaggtgacgc cgcagcgggt ggcggtgatg gagtttctcc gcggcaacac
     2881 cacgcacccg tcggttgatg atgtttatca acaggttcgg aagcggtttc cctcgatttc
     2941 ccgggccacc atctataaca cggttacgac cctggttgaa gctggcctcc tgcaggagat
     3001 tcttatccag caggaaaaaa cccacgtgga ttggaacgtg gaccggcacc accattttca
     3061 atgtctgaaa tgtgggacgg tcaaagacat ccgttatgat gcggtgaccg ctgagcaggt
     3121 atccaaacac gtggttgggc acctggtgac cggagtgaag gtggtgatgg aaggggtgtg
     3181 cgagagctgc cggtaagggg aaattttttt gtggggtatt tagactgaat attatctagg
     3241 atcacgttgt tttacccgtg accaatctga gatgagcgat ttcttcgctc atccggcgaa
     3301 tcgccaagcg ttcgacggtt gctgtagaca gcgcgatcac acggtggtgt tcggcatccc
     3361 ccgagggagt gacgtggctt agtcgtttgt tccgcgcaat tgttccaatc gcggagtagc
     3421 ccgggagcgg cgcgacagaa acaatacgcg cggcacgggc ttcagcgtca ggtcggatgg
     3481 gggaaggggc gaattggatg gccgtcccgg tcgaatgaca acacggatgc gaggagacaa
     3541 tcgaatggag cgtagttcgc agatgaggaa gagagggaaa ttgttcgcgg gcggaacgat
     3601 cggtttcatg tgcgcggcgg cggtcgcgtt gggtgccgtg gtcccgggag ttgcgcgcgc
     3661 ccaggcgacg gatctggtca aagaggccaa caacttcttc aagccgctcc ccgatgcgat
     3721 gccggctccc aaggacaacc ccaccacgac ggttcgggcc acgttgggga agatgttgta
     3781 cttcgagccc cggctctcca agaccaacac gatcagctgc aactcgtgtc ataacctggc
     3841 cacgggcggg gtcgacaatc tgccgacgtc aatggggcac ttggctcagt tcggccctcg
     3901 taattcaccc acggtcttga atgcggggct tcagatctcg cagttctggg acggtcgcgc
     3961 cgcctcgctg gaggatcaag ccaagggacc gattctcaat ccgctcgaga tggcgatgc
//
LOCUS       DBNGTT010000239         2774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2861601, whole genome shotgun sequence.
ACCESSION   DBNGTT010000239 DBNGTT010000000
VERSION     DBNGTT010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2861601"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..618
                     /locus_tag="AC16BC_05315"
     CDS             181..618
                     /locus_tag="AC16BC_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3241072.1"
                     /translation="MQTMRTLAGSAIVVAAMAAAAVVMGSWSGTAGGEEPSQTVDITI
                     RDSAFKVETRVLRLQAPIRIVLHNTDKIEHGFTSPGLAEVDVRVEAHGVVTYGRGIKG
                     LYVGPGEEAAIIFEPHNAGALKFSCDLHPQMKGELAVLTIGAA"
     gene            complement(891..1748)
                     /locus_tag="AC16BC_05320"
     CDS             complement(891..1748)
                     /locus_tag="AC16BC_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3241073.1"
                     /translation="MARPLRLEFPGALYHVTARGNAQQAIYVDDEDCRAFCRCLAVAV
                     ERYDWRVHAYCLMDNHYHLVIETLRPTLARGMRHLNGTYTQRVNRRHHRVGHLFQGRY
                     KAILVEREPYLLELCRYVVLNPVRANACRRPEQWRWSSFRATAGLESPPAWLTTEWVL
                     AQFGTTKGRAQTAYRTFVSDRAPQRPWDALRNQIYLGTETFVDSLPLTPTSMAEVPRA
                     QRHRQRPTLAALLRTRRPSPQAIAVAYREHGYRLREIADHCGVHYATISRRLRAAEDG
                     AGAGPTPNS"
     gene            1859..>2774
                     /locus_tag="AC16BC_05325"
     CDS             1859..>2774
                     /locus_tag="AC16BC_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HIR3241074.1"
                     /translation="MRDSSLMAIPLPAKGTVLPNINGEIPINRFLGEPGSGQATLTRT
                     KIGYELQHRFNETMSLRQNVSLNRLEQRWPDLYYNASLDADERTLYRYPYDYRETLDR
                     MAVDTALEAKLETGPIAHTIIGGIDYYRTRSDSGSRQIDYSDFPGSYRAIDVFNPVYG
                     ATLPTYVTSTASIGKTHVTGLYLQDQAKIERVTVTAGGRFDWSGSETGPGNDSVTGFT
                     MRGGAAYEVTPGATVYANYSESFNPQWFSRDASGNVVDPETGENIEAGVKTLLMNGRI
                     DTLLAVYQLTRQNIATANPATPDPFDSIA"
BASE COUNT          531 a          857 c          919 g          467 t
ORIGIN      
        1 ggagtatgcg cgagtgcaat tggcggcgtt gcccgaggcg ttacgggaat tacaagggga
       61 cgcatcctat ccggtgcggg tgtcgccgtc gatccgcgcg ctggccgacg cggtggaccg
      121 tgcgaaccag tgatggacgt cggggaacag gtcggagttg atgacaacag gagggaaacg
      181 atgcagacaa tgaggacgtt ggcgggatcg gcgatcgtgg tggcggccat ggcggcggct
      241 gcggtggtca tggggtcgtg gagcggcacg gcgggcgggg aggagccgag ccagaccgtg
      301 gacatcacca tccgcgattc cgcgttcaag gtcgagacgc gggtgttgcg tctgcaagcg
      361 ccgattcgga tcgtcttgca caacacggac aagatcgagc acgggttcac ctccccgggc
      421 ttggccgagg tggacgtgcg cgtcgaggcc cacggggtcg tgacctacgg ccgcgggatc
      481 aaaggcctgt acgtgggacc gggcgaggag gccgcgatca tcttcgagcc ccacaacgcg
      541 ggcgcactca aattcagttg cgacctgcac ccgcaaatga agggtgaact ggcggtgctg
      601 accatcggcg cggcgtagcg atctgcggca tgggcggagg cggagcgcct cgcccccccg
      661 gactctcgac gccgtcccgc gttcgtcgcg atccggccgg ttaccgcgta tcccggcggt
      721 cgacgcactc gcagtacgcg ccgagattga aacgtccacc gcccctaaag cgaaggcgtc
      781 cctcgtcgga gccgttggca agacacgtct gccggcacgc ctggacgcgg tccagccaga
      841 caaacccgaa gccagaaatt ggggtcaggt cttgcaatac aacatccccc tcaggaattg
      901 ggtgtggggc ctgccccagc cccgtcttcg gctgctcgga ggcggcggct gatggtcgcg
      961 tagtggacac cgcagtggtc cgcgatctcc cgtaaccgat agccatgctc gcgatacgcc
     1021 acggcaatgg cctgcggcga tggccgccga gtcctcagca gcgccgcgag cgtgggccgt
     1081 tggcggtgcc gctgtgctcg gggcacttcg gccatcgacg tcggggtgag cgggagggag
     1141 tcgacgaacg tctccgtgcc caaataaatc tgattgcgca gcgcgtccca cggtcgttga
     1201 ggtgcacggt cgctcacgaa cgtgcgatag gccgtctgcg cgcggccttt ggtcgtccca
     1261 aattgcgcca acacccactc cgtcgtcagc caggcgggag gcgattccaa gccggccgtc
     1321 gcccggaagc tgctccagcg ccattgctcc ggcctgcggc aggcgttggc ccgcaccgga
     1381 ttgaggacga cgtaccgaca taattccagc agatacggct ctcgctcgac caaaatcgcc
     1441 ttatagcgac cctgaaagag atgccccacg cggtgatgac gccggttcac gcgttgcgtg
     1501 taggtgccgt tgaggtggcg catgccgcgc gcgagggtgg gccggagagt ctcgatgacg
     1561 aggtgatagt gattgtccat gaggcagtac gcgtgcaccc gccagtcata gcgctccacc
     1621 gcgacggcga ggcagcggca gaaggcgcgg cagtcctcgt catcgacgta gatggcctgc
     1681 tgggcgttgc cgcgggcggt cacatggtac agggcgccgg ggaattccag gcgcaggggg
     1741 cgagccatgg gcggcaccct agcacggagc atgttgcatt gcaagacctg accccgatgg
     1801 tgtttctttt ggatcgaacg ggttttacga accggcgctt acggtgctca gcaactacat
     1861 gcgcgattcg agcctgatgg ccattccgct gcccgccaaa ggcaccgtcc tgcccaacat
     1921 caacggcgag attccgatca accggttcct gggcgaaccg ggatccggtc aggccacgct
     1981 tacgcgcacg aagatcggct acgaactcca acatcggttc aacgagacga tgtcgttgcg
     2041 ccagaatgtc agtctcaacc ggttggaaca acggtggccg gatctctatt acaacgcgtc
     2101 actcgacgca gatgaacgca cgctctatcg ctatccctac gactaccgcg aaaccctgga
     2161 ccggatggcc gtggacaccg cgctcgaagc gaagcttgag acgggtccga tcgcgcacac
     2221 gatcatcggc gggattgatt attaccgcac gcgctcggat tcgggttcac ggcagattga
     2281 ttactccgac ttccccggat cgtaccgggc gattgacgtg ttcaatccgg tgtacggcgc
     2341 gacgttgccg acctacgtaa cgtccaccgc gtccatcggc aaaacccatg tcaccggctt
     2401 gtacctgcag gaccaggcga agatcgagcg cgtgacagtg acagcgggag gacgctttga
     2461 ttggtcaggc tccgaaaccg gtcccggcaa cgattcggtg acgggtttca caatgcgcgg
     2521 tggcgcggcc tacgaggtga cgcccggcgc cacggtctac gccaattaca gcgaatcgtt
     2581 caatccgcaa tggttctcac gcgacgcctc gggcaacgtg gtggacccgg aaacaggaga
     2641 aaatatcgaa gctggagtca aaaccctgct gatgaacggg cggatcgaca cgttactcgc
     2701 cgtgtatcaa ctgacgcggc agaacattgc gaccgccaat cccgccaccc cggacccgtt
     2761 cgactccatc gcgg
//
LOCUS       DBNGTT010000240         6580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_306588, whole genome shotgun sequence.
ACCESSION   DBNGTT010000240 DBNGTT010000000
VERSION     DBNGTT010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_306588"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /gene="urtB"
                     /locus_tag="AC16BC_05330"
     CDS             <1..1524
                     /gene="urtB"
                     /locus_tag="AC16BC_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HIR3241075.1"
                     /translation="AVAALGQSDDPAARPLLITLREGRLARWQATGALARVVITGANA
                     SIEAGARVAVFGIDGQPLTDERDDPITVDAASLVVVEADRGVRRAVKGALDRLELFSP
                     DPAVRQAAATRLGREGGLGVGDTLAAALARESEPNVRRALDESVAFLRLADPDPSLQV
                     EAARTLGALRRAAALPQLRELKASSDAGVAAAATEAVTRIERWELFTRAVEIVFSGLS
                     LSSILLLMALGLAVTFGLMGVINMAHGELMMLGAYTTFVVQEIFVASFAPELRDWYFV
                     VSLPTSFVAAALFGIVLERSVIRFLYGRPLETLLATWGVSLILQQGARQIFGAANVDV
                     ASPAWLSGGLPVVEGVYLPYNRLFIVGLTIACVAATALVLFRTSTGLKIRAVTQNRDM
                     SACLGVRTRRVDTWTFAFGAGLAGLAGCALTLIGNVGPDLGQHYIVQSFMVVVAGGVG
                     KLVGTIAAAVSIGGLDKFLEPALGAVYGQVLILALLIVFLQRRPAGLFPAKGRHVDV"
     gene            1675..2802
                     /gene="urtC"
                     /locus_tag="AC16BC_05335"
     CDS             1675..2802
                     /gene="urtC"
                     /locus_tag="AC16BC_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HIR3241076.1"
                     /translation="MNVKGVREQWPLLALMLVFVIALPALNVGLSADSPLYVSDYTLN
                     LFGKFLAYAILALGLDLIWGYTGILSLGHGVFFGLGGYAIGMHLMLEIGADGVYQSEL
                     PDFMVWNQVTALPWFWVPFKSGVFALLAVVLVPGLFAFLFGYFTFRSRIKGVYFSIIT
                     QALALSVWLLFNRNDLNLGGTNGLADFKTIFGWSLRDTSTQRGLYLVTAVALVAAFAA
                     CRWITRSPVGKVLIAIRDAEGRARFSGYPVARYKLFVFVVSAVLAGIAGALYAPQVGI
                     ITPGRMGVLPSIEMVIWVAVGGRGTLIGAVLGAIGVNWLRSVLTESYPDVWLFVLGGL
                     FVAVVMAFPEGVVGLPKRIGSWMVGRRIGAAAESLRAPESP"
     gene            2799..3569
                     /gene="urtD"
                     /locus_tag="AC16BC_05340"
     CDS             2799..3569
                     /gene="urtD"
                     /locus_tag="AC16BC_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HIR3241077.1"
                     /translation="MSRSLLYVENVTVDFDGFKALRDLNFIMDEGELRVVIGPNGAGK
                     TTLLDVISGKVKPSAGRVVFGRDAELSTLAEHEIARAGIGRMFQTPTIYPSLTVFENL
                     ELSQARGRGVLAGLFARLSSSQRQAIAATLERVGLENKGEWRAAALSHGEKQWLELAM
                     LMVHDPELYLVDEPVAGMSGEETDRTAEILLDIAKRRSVLVIEHDMAFVRRLGAKVTV
                     LHEGQQLCEGSMEEVQRNQRVVEVYLGRERKGKASAAG"
     gene            3556..4251
                     /gene="urtE"
                     /locus_tag="AC16BC_05345"
     CDS             3556..4251
                     /gene="urtE"
                     /locus_tag="AC16BC_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HIR3241078.1"
                     /translation="MLRVEGISAGYGETRVVRDVSLEVPDGSVVCLMGRNGVGKTTCL
                     KAIIGLLRAWNGRIMLGDQDVTRCSPDERARRGIGYVPQGREIFPSLTVEENLRIGLQ
                     AGANGSDRIPDDVYDLFPALRALLTRRGGDLSGGQQQQLAFARALVARPKLLLLDEPT
                     EGIQPSIIDQIEDVIGRIKSLKHTAILLVEQYVEFAARVADAFIIMEKGTLVARGATS
                     ELSDEMVRRHLAV"
     gene            4258..4560
                     /locus_tag="AC16BC_05350"
     CDS             4258..4560
                     /locus_tag="AC16BC_05350"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HIR3241079.1"
                     /translation="MYLAPREQEKLLIVVAAQLARERRSRGLKLNYVEASALITAALL
                     EAIRDGRSVADLMAFGATILTRDDVMDGVPEMLTEIQVEGTFPDGTKLVTVHDPIR"
     gene            4589..4999
                     /locus_tag="AC16BC_05355"
     CDS             4589..4999
                     /locus_tag="AC16BC_05355"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005555675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HIR3241080.1"
                     /translation="MGEGEKGSRQIIPGEYILAEGEIVALEGRATVTLTVTNTGDRPI
                     QVGSHFHFFEANRALRFDRAKAFGFRLEIPAGTAMRFEPGEDKVATLTAFAGARRVWG
                     LNGLTNGALDDAAVRRVALDRARQEGFQGAEADP"
     gene            4996..>6580
                     /gene="ureC"
                     /locus_tag="AC16BC_05360"
     CDS             4996..>6580
                     /gene="ureC"
                     /locus_tag="AC16BC_05360"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HIR3241081.1"
                     /translation="MTLRMSRRQYADLYGPTTGDRLRLADTELIIEIERDLTVYGEEA
                     KFGGGKVLRDGMGQIPEPAGRALDLVITNAVVLDYWGIVKADIGVVDGRITGIGKAGN
                     PDLMPGVTPGMEVGPGTEVIAAEGKLVTPGGIDSHVHFICPGLIDEALAAGLTTLMGG
                     GTGPATGTNATTCTPGPWNLARMLEAAEAFPVNLGFLGKGNASQPKGLADQIEAGAIG
                     LKLHEDWGTTPAAIDACLDVAERYDVQVAIHTDTINEAGFVETSINAFKGRTIHTYHT
                     EGAGGGHAPDILRVCGEPNVLPSSTNPTMPYTVNTLEEHLDMLMVCHHLSPRIPEDLA
                     FAESRIRGETIAAEDVLHDLGAISMMSSDSQAMGRIGEVIIRTWQTAHKMKVQRGSLP
                     GDPARHDNGRVKRYLAKYGINPAIAHGIAHEVGSIEVGKLADLVVWRPAFFGVKPDLV
                     IKGGTIALAAMGDANASIPTPQPVLLRPMYGAKGRAIGSTSVTFLSRAAVERGIPQRL
                     ALTKRAVAVRGCRGVGKRDM"
BASE COUNT         1052 a         1962 c         2357 g         1209 t
ORIGIN      
        1 gcggtggcgg cgctggggca gagcgacgac cccgccgcgc gtccgctgct catcacgctg
       61 cgcgaaggcc gtctcgcgcg gtggcaagcg accggcgcgc tggcgcgggt ggtgatcacc
      121 ggggcaaacg cctcgatcga ggcgggggcg cgcgtcgcgg tgttcgggat cgacgggcaa
      181 ccgttgaccg acgaacgcga cgatccgatc acggtggacg ccgcctcgct ggtcgtggtc
      241 gaggcggatc ggggcgtgcg gcgcgcggtc aaaggcgcgc tcgatcgact tgaactgttc
      301 tcgccggatc cggccgtgcg ccaagccgcg gcgactcgac tcgggcgcga ggggggcctc
      361 ggggtggggg acacgcttgc cgccgctctc gcgcgcgaga gcgagccgaa cgtccgccgc
      421 gcgctcgatg agtcggtggc gttcctgcgc ctcgcggatc ccgatccgtc gcttcaagtc
      481 gaagcggctc gaaccttggg cgcgttgcgc cgcgccgccg cgctgccgca gcttcgcgag
      541 ttgaaggcgt cgtccgacgc cggcgtcgcc gccgcggcta cggaggccgt cacgcggatc
      601 gaacggtggg agctgtttac ccgcgcggtg gaaatcgtgt tttcgggact gagcctcagt
      661 tcgattctct tgttgatggc gctgggattg gcggtcacgt ttgggttgat gggcgtgatc
      721 aacatggcgc acggcgaatt gatgatgctg ggcgcctata ccacgttcgt ggtgcaagag
      781 atcttcgtgg cttcgttcgc gccggaactg cgggactggt acttcgtggt gtcgctgccg
      841 acctcgtttg tcgcagccgc gttgttcggc atcgtgctcg aacgcagcgt gatccgtttt
      901 ctgtacggcc ggccgctcga aaccctgctg gcgacctggg gcgtgagcct gatcctgcaa
      961 cagggcgcgc gccagatctt cggggccgcc aacgtggacg tggccagccc ggcgtggttg
     1021 tccgggggac ttccggtcgt cgagggcgta tatctgccct acaaccggct gtttatcgtc
     1081 ggattgacca tcgcctgcgt cgctgccacc gcgctggtgt tgttccgcac ctccaccgga
     1141 ctgaagatcc gcgccgtgac ccaaaatcgc gacatgagcg cgtgtctggg cgtgcgcacc
     1201 cgccgcgtag acacgtggac cttcgcgttc ggggcggggc tggccggctt ggcgggatgc
     1261 gccctcacct tgatcgggaa cgtgggcccg gatctgggcc agcactacat cgtgcagtcc
     1321 tttatggtgg tcgtggcggg cggggtcggg aagctggtcg ggaccattgc cgcggcggtg
     1381 agcatcggcg ggctggataa gttcctggaa ccggccctcg gcgcggtgta cggccaggtg
     1441 ctcatccttg cgttgctgat cgtgttcctg caacgacgac cggcaggtct tttcccggcc
     1501 aaaggccgcc acgtcgatgt ctaggctgag cctgtccatg accggccatc tgcggcgttg
     1561 tcgctgcgcg tccgctcctc acgtacgacc cagtacgctg cggtgcgggc gctcgctccg
     1621 ccttgcatct ggcgcggtcc tgaacaggct cccatacttc gtatggggac ggatttgaac
     1681 gtcaagggag tgcgcgagca gtggccgctc ttggcgctga tgcttgtctt cgttatcgcg
     1741 cttccggcgc tgaacgtggg gttgtcggcg gactcgcccc tgtacgtgtc cgattacacg
     1801 ctcaacctgt tcgggaagtt cctggcgtac gcgattctgg cgctggggct ggatctgatt
     1861 tggggataca ccggcatctt gtcgctcgga cacggcgtgt tcttcgggct cggcggctac
     1921 gcgatcggaa tgcacttgat gctcgagatc ggcgccgacg gtgtgtacca gagcgaactc
     1981 cctgacttca tggtgtggaa ccaggtcacc gcgttgccgt ggttctgggt gccgttcaag
     2041 agcggcgtgt tcgcgctgct cgcggtggtc ctggtcccgg ggctttttgc attcttgttc
     2101 ggctacttca cgttccgcag tcgcatcaaa ggggtgtact tctcgatcat tacccaggcg
     2161 ctcgcgttgt cggtctggtt gctcttcaac cggaacgatc tcaacctcgg aggcaccaac
     2221 ggtttggccg atttcaagac gattttcggg tggtcgctgc gcgacacgtc cacccagcgg
     2281 ggcctctacc tcgtgaccgc ggtcgcgttg gtggcggcgt tcgcggcgtg ccgatggatc
     2341 acccggtcgc cggtcggcaa agtcttgatc gcgatccgcg acgcggaggg ccgtgcgcga
     2401 ttctccggct atccggtggc ccggtacaaa ctgttcgtgt tcgtggtgtc ggcggtgctg
     2461 gccgggatcg cgggcgcgtt atacgcgccg caggtgggca tcatcacgcc gggccggatg
     2521 ggcgtgttgc cgtcgatcga gatggtcatc tgggtcgcag tgggaggccg tggaacgttg
     2581 atcggggcgg tgctgggcgc aatcggggtg aactggctgc gcagcgtgtt gaccgagtcc
     2641 tatccggacg tgtggctctt cgttctcggt gggttgttcg tcgcggtggt catggcgttt
     2701 ccggagggag ttgtgggcct tcccaaacgc atcggctctt ggatggttgg acggcgcatc
     2761 ggcgccgcgg cggaatcgct ccgagcaccg gagtcgccgt gagtcgctcg ctgctctacg
     2821 tcgagaacgt gaccgtggac ttcgacgggt tcaaggccct gcgcgatttg aacttcatca
     2881 tggacgaggg ggaattacgg gtcgtgatcg ggcccaacgg cgcgggaaaa accacgctgt
     2941 tggacgtcat ctcgggcaag gtaaagccgt ccgcgggccg ggtcgtcttc gggcgcgacg
     3001 cggaactttc cacgctcgcc gaacacgaaa tcgcgcgcgc cggcatcggg cggatgtttc
     3061 aaacgccgac gatttacccc tcgctcaccg tgttcgaaaa tttggagctg tcgcaggcgc
     3121 gcgggcgcgg cgtgctggcc gggttgttcg cgcggctctc ttcgtcccag cgtcaggcca
     3181 tcgccgcgac gctcgaacgg gtcggcttgg agaacaaagg cgaatggcgg gcggccgcgt
     3241 tgtcgcacgg ggagaaacaa tggctcgaac tggccatgct catggtccat gaccctgagc
     3301 tgtatttggt cgacgagccc gtcgcgggga tgagcggaga ggaaaccgac cgtaccgcgg
     3361 agatcctgct ggatatcgcc aaacgtcgct cggtcctcgt gatcgagcac gacatggcgt
     3421 tcgtgcggcg gctcggcgcc aaggtgacgg ttctgcacga agggcagcag ctctgcgagg
     3481 gctcgatgga ggaggtccag cgtaatcagc gcgtggtcga ggtgtacctc ggccgggaac
     3541 gaaagggcaa agccagtgct gcgggttgag ggcatctcgg ccgggtacgg cgaaacccga
     3601 gtcgtgcgcg acgtctcgct ggaggtgccg gacgggtccg tggtctgttt aatggggcgc
     3661 aacggggtgg ggaaaaccac ctgtctgaag gcgatcatcg gcttgcttcg cgcctggaac
     3721 ggacggatca tgctgggaga tcaagacgtc acccggtgtt cacccgacga gcgcgcgcgt
     3781 cgcggcatcg ggtacgtccc gcagggccgg gagattttcc cctcgttgac cgtggaggaa
     3841 aatcttcgca tcggattgca ggccggggcg aacggatccg acaggattcc ggacgacgtg
     3901 tacgatctgt ttcccgcgtt gcgcgccctg ctgacgcgcc gcggcggaga tttgagcggg
     3961 ggccaacagc agcagctcgc gttcgcgagg gcgttggtcg cccgtcccaa gctgttgttg
     4021 ttggacgagc cgaccgaggg cattcaaccg tccatcatcg accagatcga agacgtgatc
     4081 gggcggatca aatcgctgaa gcacaccgcg attctcctgg tcgaacagta cgtggagttc
     4141 gcggcccggg tggccgacgc ctttatcatc atggaaaagg gcacgctggt ggcgcgcggg
     4201 gccacgtccg aactctcgga cgagatggtg cgacgccacc tggcggtgtg agcgggtatg
     4261 tacctggcac cgagggaaca ggagaaactg ctgattgtcg tggcggccca actcgcgcgc
     4321 gagcggcgca gccgcggcct taagctcaat tacgtggagg cgtcggcgct gatcaccgct
     4381 gcgttgctcg aggccattcg cgacgggcgg agcgtcgccg atctgatggc gttcggcgcc
     4441 acgattctga cgcgggatga cgtgatggac ggcgtgccgg aaatgttgac cgaaattcaa
     4501 gtggaaggca cgttcccgga cggcaccaag ctcgtcacgg tgcatgaccc gatccggtga
     4561 cggaagaatc gatggggaga aggggggaat gggtgagggg gagaaagggt caagacagat
     4621 aattccgggc gagtacattc tggccgaggg cgagatcgtg gcgctggagg ggagggccac
     4681 ggtgacgttg accgtgacca acaccggtga ccgaccgatt caggtcggat cacacttcca
     4741 tttctttgag gccaatcgcg cgctgcgatt cgaccgcgcc aaggcgttcg ggttccgact
     4801 ggagatcccg gccggcacgg cgatgcgatt cgaacctggg gaggacaagg tggcgacctt
     4861 gaccgcgttt gccggggcac gaagggtgtg ggggctcaac gggctgacca acggcgcgct
     4921 ggacgacgcc gcggtgcgcc gggtcgcgct ggaccgtgct cgtcaggagg gattccaagg
     4981 agcggaggcc gacccgtgac cctgcggatg tcccgacgcc agtacgcgga cctctacggc
     5041 ccgaccaccg gcgaccgtct gcgtctggcc gacacggagt tgatcatcga aatcgaacgc
     5101 gacttgaccg tgtacggcga ggaggccaag ttcgggggcg gcaaggtgtt gcgcgacggg
     5161 atggggcaga tcccggagcc agccggtcgc gccctcgacc tcgtaatcac caatgcggtg
     5221 gtcctcgact actggggcat cgtcaaagcc gacatcgggg tcgtggacgg ccggatcacg
     5281 ggcatcggca aggccgggaa tccggatctg atgccgggag tcacgccggg catggaggtc
     5341 ggacccggta ccgaggtgat cgccgcggag ggcaaactgg tgacgcccgg cggtatcgac
     5401 tcgcacgtgc acttcatctg ccccggcttg atcgatgagg cgctggccgc gggactcacc
     5461 acgctgatgg gcggcggaac cgggcccgcg accggcacca acgccaccac gtgcactccg
     5521 gggccgtgga atctcgcccg tatgctcgag gccgccgagg cgtttccggt caatctcgga
     5581 tttctcggca aagggaacgc gtcacagccc aagggtttgg ccgaccagat cgaggccggc
     5641 gcgatcggcc tgaagttgca cgaggactgg ggcactacgc ccgccgccat cgatgcgtgt
     5701 ctggacgtgg ccgagcggta cgacgtacag gtcgccattc acaccgacac gatcaacgaa
     5761 gcggggttcg tggagacctc gatcaacgcg ttcaagggcc gcacgatcca cacctatcac
     5821 accgaaggcg cggggggcgg gcacgccccg gatatactgc gcgtgtgcgg cgagcccaac
     5881 gtgctgccgt cgtccaccaa ccctaccatg ccgtacacgg tcaacaccct ggaggaacac
     5941 ctcgacatgt tgatggtgtg ccatcacttg agcccgcgga tccccgagga cctcgcgttc
     6001 gccgagtcga ggattcgggg agaaaccatc gcggccgagg acgtgctgca cgatctgggc
     6061 gcgatcagca tgatgtcttc ggattctcag gccatggggc ggatcggcga ggtgatcatc
     6121 cgcacctggc agaccgccca taagatgaag gtgcagcgcg ggagcctacc cggcgatccg
     6181 gcgcggcacg acaacggccg ggtcaagcga tatctggcca aatacggcat caatcccgcg
     6241 attgctcacg gcattgcgca tgaggtgggg tcgatcgagg tcggcaagct cgcggatctg
     6301 gtggtgtggc gtccggcgtt tttcggcgtc aagcctgacc tggtgatcaa gggcgggacg
     6361 attgcgctgg cggcgatggg cgacgccaac gcgtcgatcc cgacgccaca gccggtcctg
     6421 ctgcggccga tgtatggcgc aaagggtcgc gcgattggat cgacttccgt gacgtttctg
     6481 tctcgtgcgg cggtcgagcg cgggattccc cagcggctgg cgttgacgaa acgggccgtc
     6541 gcggtcaggg gctgccgcgg cgtcggcaaa cgggacatga
//
LOCUS       DBNGTT010000241        12157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1456951, whole genome shotgun sequence.
ACCESSION   DBNGTT010000241 DBNGTT010000000
VERSION     DBNGTT010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12157
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1456951"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..723
                     /locus_tag="AC16BC_05365"
     CDS             433..723
                     /locus_tag="AC16BC_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241082.1"
                     /translation="MVIEIHQVEGFDPQHRSFNRNAEINRSEQGLSARFFYEGLRVDT
                     GTFFEVDRVMTDLVERLRKLGFTLLRSRVNFRGSRYLAERQPWIDYPDAVVR"
     gene            complement(738..1226)
                     /locus_tag="AC16BC_05370"
     CDS             complement(738..1226)
                     /locus_tag="AC16BC_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR3241083.1"
                     /translation="MKVTPVDIQQIGFRRAFRGYDRHEVDAFLSSLSETFESLMKENR
                     ESKDRIEALEAALAELKRKEELINQTLVSAERMVDDMKRQAQREAELRVREAEVAADT
                     LTRKAREERHDIEQDVIAWRRRQIELVEQFRGFLRGIEKSLDLATESTHREETVGTPR
                     VA"
     gene            complement(1306..1605)
                     /locus_tag="AC16BC_05375"
     CDS             complement(1306..1605)
                     /locus_tag="AC16BC_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR3241084.1"
                     /translation="MFVAGNFLSAVATVLDMVLEFYKWVVIIAALLSWVNPDPYNPIV
                     QFLRKITEPVLYPIRRAMGGYGLGIDLSPLVVILAIIFLQQFLVHTLRQAALQFH"
     gene            complement(1615..2358)
                     /locus_tag="AC16BC_05380"
     CDS             complement(1615..2358)
                     /locus_tag="AC16BC_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR3241085.1"
                     /translation="MAAETVADRLADVRSRIARAAAAVGRDPGEIRLIGVSKGMGIDA
                     IREAAAAGLSDFGENRVQEALEKMTALGSEGSNGCSWHLIGRLQTNKVRAAVGRFSLI
                     QSVDSARLGHAIDEAAGRTGIVQRVLIQVNIGREAQKGGVDPSDLESLWRELTTCRHL
                     RVEGLMAIPPAGDDPETARPYFRELRRLGDPLPAPTGDRRPLEYSMGMSHDFEAAVKE
                     GATMVRVGTAIFGPRSLAGDRSLTAGNGG"
     gene            complement(2358..3086)
                     /locus_tag="AC16BC_05385"
     CDS             complement(2358..3086)
                     /locus_tag="AC16BC_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR3241086.1"
                     /translation="MAAHRFESGAEGEWIVLDGFSAPHRFGTRRFAASAVRALDGEPV
                     LTARQVHKDGILGVPSGDRGAAQWQTEAASADADGVATGRADCWVGVVTADCVPLLLH
                     DPAARAVAAVHAGWRGALMGVAPAAVRALVARFGAHPSRIEAAIGPHIGACCFEVGHA
                     VLDPLSNTPGWERWIARRAGDKGYFDLGAFVRWQLEEVGVAPERVTALGLCTRCDPSR
                     FSSYRREGRRPPGMLSAVRVGSRA"
     gene            complement(3096..4259)
                     /gene="ftsZ"
                     /locus_tag="AC16BC_05390"
     CDS             complement(3096..4259)
                     /gene="ftsZ"
                     /locus_tag="AC16BC_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3241087.1"
                     /translation="MFLFDEDKQETVPARIIVMGIGGGGCNAISTMIAGGLGQVEFAV
                     ANTDLQVLRSSAAGAKIQLGARLTKGLGAGANPQIGRDAAIEDSERIREVLSGADMVF
                     ITAGMGGGTGTGAAPVIAGIAKELGALTVAVVTKPFTFEGARRMRQAEDGVRELRNSC
                     DTLLVIPNQRLLSVVEKGTPLTRAFGVADDILRQAVQGIAEVITVPGLVNVDFADVRA
                     IMTHMGRAVMGMGRARGEHRAMEAAQRAIASPLLEDGSIQGARGVLINITGGPDLSLH
                     EVTEASSIIQEAADPDANIIFGSVINEQMSEDILVTVIATGFDAPAQGPAGSRQAVEA
                     PSHGSTTPGVAPPVRTLTIEATPPLRKPSGVKSRDGLTEEEWDVPTFLRKQLD"
     gene            complement(4301..5548)
                     /gene="ftsA"
                     /locus_tag="AC16BC_05395"
     CDS             complement(4301..5548)
                     /gene="ftsA"
                     /locus_tag="AC16BC_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3241088.1"
                     /translation="MAKRDQIVVGLDVGTTKICAIVGEVSEGAIPGERPHVKIIGIGT
                     HPSRGLRKGVVVNVESTIESIKKAVEEAELMAGVEINSVYTGIAGSHIKGFSSRGVIA
                     IKNREVTAADVKRVVEAAKAVAIPMDREILHVLPRGFAVDDQEGIKDPIGMFGTRLEV
                     EVHIITGAVTSAQNIVRSINRAGLEVVDLILQPLASSEAVLTAEERELGVAMVDIGGG
                     TTDLATIVDNGVWHTAVLGIGGNHVTNDIAVGLRTPAYEAEKIKIRYGCASTSMVRDH
                     ETIEVPSMGGRPPRVLSRQLLSEIIEPRAEEMFSLVAQELRKMGYEDRVAAGVVITGG
                     TSILDGMPELAERVMNLPVRRGAPTDVGGLVDIVNSPMYATGVGLILYGGKHREPSRT
                     TRSGNGTWQRVVRTMKGWVQEFF"
     gene            complement(5651..6391)
                     /locus_tag="AC16BC_05400"
     CDS             complement(5651..6391)
                     /locus_tag="AC16BC_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3241089.1"
                     /translation="MKRRRWIRRGLWAVVLCATGVTAAGWSWSAVCGTGAGLCGWDVI
                     ERVEIRGTVRLDERQVRAWAAVPMGASLWTLDGDEVVARLDGRPWVRAATVGKRFPDT
                     VVVTVTERVPAAVAVTDRGRVLVDADGRVIGPTALDRGFPVVRGGAAVRPEALVTAAQ
                     ILAAFRVAEVRIAAVESLVIDVSTPDDPIVELPGAMRVRFGREGFIEKWRRVQAIRGN
                     VALRITGPKMVDLRFSDRAVVSEWSGAL"
     gene            complement(6388..7296)
                     /locus_tag="AC16BC_05405"
     CDS             complement(6388..7296)
                     /locus_tag="AC16BC_05405"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR3241090.1"
                     /translation="MTTAVRVGVLMGGRSAEREVSLRSGAAVLAALQRRGFDAVGIDV
                     DAGVADRLIRERVGLAFIALHGRYGEDGTIQGLLEIVGIPYTGSGVLASALGMDKLIS
                     RALFAAAGLPVPRYVLLTEPTPPSLPFAFPVVVKPNSEGSSVGVNLVSGAHELPAAIA
                     EAFRYDRRVLIEAYIAGKEVQVGILGDRALGAIEIRSKTAFYDYTAKYTPGMSEHIFP
                     ATLPPDVMRRTLDVGLAAFRALRAEGYGRVDCLVDQAGTPYVLEVNTLPGMTETSLLP
                     DIARGVGLSFDDLVEAIVRAALERGT"
     gene            complement(7293..8213)
                     /gene="murB"
                     /locus_tag="AC16BC_05410"
     CDS             complement(7293..8213)
                     /gene="murB"
                     /locus_tag="AC16BC_05410"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3241091.1"
                     /translation="MTTRSSVAVRPSLRDTLSGVRGEVRFDEPLAKYTSMKVGGPADA
                     LVTPADEADLRSALVAAKAARVPVMVLGGTNLVVRDGGVAGVVLRLSGLKSIRPAGVN
                     RLYAEAGALFPTLSHAAKDAGLSGLEFACGIPGTVGGAVVMNAGTRDGEIADVLESVR
                     LMTWEGEVVELAASKLGLRYRESHIPPGVLVGATVVLKPDDPHAIDSRMQTILEYRRR
                     TQPLNLPNAGCIFKNPPGRSAGQLIDELGLKGYRVGGIQVSERHANFMVNVGEGTAKD
                     LAALIRHVGGLVQRERGMTLELEVRLVGRA"
     gene            complement(8374..9738)
                     /gene="murC"
                     /locus_tag="AC16BC_05415"
     CDS             complement(8374..9738)
                     /gene="murC"
                     /locus_tag="AC16BC_05415"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3241092.1"
                     /translation="MFKKIQRLHFVGIGGSGMSGIAEVLLTLGYRVTGSDLAASEVTR
                     RLESLGGTVYIGHRAEQVEGAQVVVTSSAVSPANVEVAAARARMIPVIPRAEMLAELM
                     RLKYGIAVAGAHGKTTTTSMIATVLAHGGLDPTAVIGGKLNRFGGHAKLGQGEFLVAE
                     ADESDGSFLKLSPTIAVVTNIDREHLDHYQDLEAIKRAFLDFVNKVPFYGAAVLCLDE
                     PNLQALIPKIEKRVRTYGRSPQADLTATSIRLSPKGAEFHARLGASDLGRFNLTVPGA
                     HNVSNAMAAILVGLELDLPVDAIRDGLAQFSGVERRFQMRGERQGVLVVDDYGHHPTE
                     IRATLAAAKEGWGRRLVVVFQPHRYSRTRDLFADFCTAFYQADVLLVLDVYAAGESPI
                     EGVNSQALVAAIREHGHRDATWMESRDAAVERIAQVVQAGDMVITLGAGDVWKIGPAF
                     LERP"
     gene            complement(9747..10814)
                     /gene="murG"
                     /locus_tag="AC16BC_05420"
     CDS             complement(9747..10814)
                     /gene="murG"
                     /locus_tag="AC16BC_05420"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR3241093.1"
                     /translation="MRVVIAGGGTGGHLYPGIALAHVFLRARPGSSVLFVGTERGLES
                     RVVPREGFELATIRVRGLVGRGVVRAAATIARLPVSLLEAVRVLRRFRPDLVVGVGGY
                     AAGPTIAAARLLRRTIVLLEQNVLPGVTNRWLAPVADLVVASFEESRAAFRGRVEVLG
                     NPVRPAVVSAGRTAVPDAPSILVFGGSQGARAINRAVVGALPALSKITGLSIVHQTGQ
                     ADFAETKQAYEAAGVTATVTPYVDDMGAAYARAALVVARAGATTVAELTACGRPAILI
                     PYPHAAHGHQERNARAVEAAGAAVTVVERDLTGAMLGEAIADLLADRPRLLRMAEASR
                     ALGRPDAGARIAAACLALVER"
     gene            complement(10930..12078)
                     /gene="ftsW"
                     /locus_tag="AC16BC_05425"
     CDS             complement(10930..12078)
                     /gene="ftsW"
                     /locus_tag="AC16BC_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR3241094.1"
                     /translation="MRARAQRHPASAWLNGRLDAREWGRGDGVLALTAGVLVVIGLVM
                     VYSASSAIATTRYDDATYFVMRQAAWAVVGLAAFGLGALIDYHVWRRFVVPLTLGTVV
                     ILAVVLTMGWSVNGSRRWLRAGGFTIQPSEFARVIFIVYAAHMLAKRADVIRSFKTLA
                     PLLVVVGVGVLLIVAEPDLGNAVVLATVTAALCVLGGARMSHLGALAACAVPVVAYAI
                     LGTAYRRRRLMAFLDPWSDASDSGFQVIQSMLSFGAGGTMGAGLGEGRQKLFFLPEPH
                     TDFIYSVVGEELGLWGTAAILALFAVLLWRGWRVALATTDPFGRLLAFGLSVAVGLAA
                     ALNIGVATGMLPTKGLPLPLVSYGGSSLVANLFALGVVFNISRQRGIK"
BASE COUNT         2093 a         4580 c         3619 g         1865 t
ORIGIN      
        1 gaggaccaac ccgacgaaga ccggaacaca caaaatccgg agaagggtca aggcattggg
       61 tatgttcact cgctacgccc cggggtcatc cgattgcccc tccacgatcg ccctgatcca
      121 cattcactgt ggacaacgtg tgggtaatgt ggagaaatat gcgcggatca cgcatgaaga
      181 gtgcgggtcc gagcaacttg cccaaaattt gggcaaacaa tcctacggta tttcaaacga
      241 ttcggaagac acgcgctgtt cgacatcgcg ccggcgccgg tccgggagat gccgaacgtg
      301 gcgcgcccaa ccccgtgatc ctgcttcggc aaccccgtct tcacgggcgg aacagggctt
      361 gccctccatc cgggaagccg ggtataatcc ggccctcttg gatgggctga accttcgcag
      421 gggagagtat gcatggtgat tgagatccac caggtggagg gcttcgatcc tcaacatcgg
      481 tcgttcaatc ggaacgcgga gatcaaccgc agcgagcagg ggttgtccgc ccgatttttc
      541 tacgaaggcc tccgcgttga taccggaacg tttttcgaag tggaccgcgt catgaccgat
      601 ctggtcgagc gcctcaggaa attgggattt acactgctcc gctctcgcgt caatttcagg
      661 ggctcccgct atctggccga gcgtcagccc tggatcgatt atcccgacgc tgtggttcgc
      721 tgagcgactc ggcccgttta cgccacgcgg ggcgtgccga ccgtttcctc gcggtgagtc
      781 gattccgtgg ccaggtccag gctcttttca atccccctga gaaacccgcg aaactgttcc
      841 accaattcga tctgccggcg acgccaggca atcacgtcct gttcgatgtc gtgacgttct
      901 tctcgggctt tccgggtcaa cgtgtccgcg gcgacttccg cctcgcggac ccggagttcg
      961 gcttcccgtt gcgcctggcg cttcatgtcg tcaaccatcc gctcggctga caccagcgtc
     1021 tgattgatca gttcctcctt gcgtttgagc tctgcgagcg ccgcttcgag cgcctcgatc
     1081 cgatccttgg actcgcggtt ctccttcatc aaggactcga acgtctccga gagactggac
     1141 agaaacgcgt ccacctcgtg acgatcatac ccgcggaagg ctcgacgaaa gccgatttgc
     1201 tgaatatcaa ccggtgtcac cttcatggag ttcaccctcc ttatctcatc tccgcatcga
     1261 gaccgtcgtc cgctccaacg gacggaccgc ggcccaaccg gaccttcagt gaaactgcag
     1321 cgccgcctgc cgcaacgtat ggaccagaaa ctgctgcaaa aagatgatcg cgaggatcac
     1381 caccagcgga gacaggtcga ttcctaatcc gtacccgccc atggcgcgac gaatcggata
     1441 aaggaccggc tcggtgattt tacgcaaaaa ctgcacgatc ggattgtagg ggtcgggatt
     1501 gacccatgac aagagcgccg caatgatcac cacccacttg taaaactcca gcaccatgtc
     1561 caataccgtg gccacggccg acaaaaaatt tcccgctacg aacatatttc tcccctaccc
     1621 gccgttgcca gccgtcagcg atctgtcccc agccaatgac cggggtccaa agatcgcggt
     1681 cccgacccgg accatggtcg ccccttcctt caccgccgcc tcaaagtcgt gactcatacc
     1741 catcgagtac tcaaggggac ggcggtcccc ggtcggcgca ggcaggggat cgcccaagcg
     1801 gcgcagttcc cgaaaatacg gccgcgccgt ctcaggatcg tctcccgccg gaggaatcgc
     1861 catcaaccct tcgacgcgca gatgccgaca ggtcgtgagt tcccgccaca gggactccaa
     1921 atccgacgga tcaacgccgc ctttctgcgc ctcgcgcccg atgttgacct gaatgagcac
     1981 gcgctggacg ataccggtgc gccccgcggc ctcgtcgatg gcatgcccca gccgcgcgct
     2041 gtcgaccgat tgaatcaggc tgaatcgccc caccgcggcg cggaccttgt tggtctgcag
     2101 ccgaccgatg aggtgccacg agcacccgtt cgatccctcg ctcccgagag ccgtcatttt
     2161 ttcgagcgct tcctggaccc gattctcgcc gaagtcggac agcccggccg ccgcggcttc
     2221 gcgaatcgcg tcgatcccca ttcccttgga cacgccgatc aggcggatct cgccgggatc
     2281 gcgcccgaca gccgccgcgg cgcgcgcgat ccgcgagcgc acgtccgcaa ggcggtcggc
     2341 gacggtctcc gccgccatca cgcccgcgac ccgacccgca ccgcgctcaa catccccggg
     2401 ggacgacgcc cttcgcgtcg gtaggaggaa aaccgggacg ggtcgcagcg cgtacacaac
     2461 cccagcgccg tcacccgctc cggagcgacg ccaacctctt ccagctgcca tcgaacgaac
     2521 gcgcccaggt cgaaatatcc cttgtcgccc gcccggcgcg cgatccaacg ttcccacccc
     2581 ggcgtgttgg acagcgggtc cagcacggcg tgtcccacct cgaagcagca cgcgccgata
     2641 tgcgggccga tcgcggcctc gatgcgcgag gggtgagccc cgaaccgagc caccagcgcc
     2701 cgcaccgccg cgggcgccac gcccatcagc gccccgcgcc acccggcgtg caccgcggcc
     2761 acggctcgcg ccgcgggatc gtgcaacaac aacggcacgc aatccgccgt gaccacgccg
     2821 acccagcaat cggcgcggcc ggtggccacg ccgtccgcgt cggccgacgc cgcctcggtc
     2881 tgccactgcg cggcgccccg gtcgccggac ggcacgccga gaatcccgtc cttgtgcacc
     2941 tgtcgcgcgg tgagcacggg ctcgccgtcg agggcgcgca ccgcggaggc cgcgaagcgg
     3001 cgggtcccga agcggtgcgg cgcggagaat ccatcgagga cgatccactc gccctctgcc
     3061 ccggattcaa atcggtgggc ggccacggcc atgcctcagt ccaattgttt ccgcagaaac
     3121 gtcggcacat cccactcctc ttcggtcaac ccgtcgcgcg atttgacgcc actcggcttc
     3181 cgaagcggcg gagtcgcctc gatggtcaat gtcctgaccg gcggcgcgac gccgggcgtg
     3241 gtcgacccgt gcgagggcgc ctcaaccgcc tgacgggagc cggccggccc ctgggccggc
     3301 gcgtcgaacc cggtggcgat cacggtgacg agaatgtcct ccgacatctg ttcgttgatc
     3361 acggacccga aaataatgtt ggcgtcggga tcggccgcct cttgaatgat cgaggaggcc
     3421 tcggtgacct cgtgcaggga caggtcggga ccgccggtga tattgatcaa cacgccgcgc
     3481 gcgccctgga tggagccgtc ttccaacaac gggctcgcga tcgcgcgctg cgcggcttcc
     3541 atcgcgcgat gttcgccgcg ggcgcggccc atgcccatca cggcgcggcc catgtgcgtc
     3601 atgatcgccc tcacgtccgc gaaatccacg ttcacgaggc cgggcaccgt gatcacctcg
     3661 gcgatcccct gcaccgcctg gcgcaaaata tcatcggcga cgccgaacgc gcgtgtcaag
     3721 ggcgtgccct tttcaaccac gctcaggagc cgctgattcg ggatcaccag caacgtgtcg
     3781 cacgagttgc gcagctcgcg caccccgtcc tcggcctgac gcatccggcg ggcgccctcg
     3841 aacgtgaacg gcttcgtcac caccgcgacc gtcaacgcgc ccagctcctt ggcgatgccc
     3901 gcgatcacgg gcgcggcgcc ggttccggtc cccccgccca tcccggcggt gatgaacacc
     3961 atgtcggcgc cggagagcac ctcgcggatg cgctccgagt cttcgatggc cgcgtcgcgt
     4021 ccgatctgcg gattcgcgcc ggcgcccagc cccttcgtca accgcgcgcc caactgaatt
     4081 ttggcgccgg cggccgacga acgcagcact tgaagatccg tgttcgccac cgcgaactcc
     4141 acctgcccca gaccgcccgc gatcatcgtc gagatggcgt tgcacccgcc gcccccgatc
     4201 cccatcacga tgatccgggc gggcaccgtc tcctgcttgt cttcgtcgaa taggaacatg
     4261 ctgaccccct ccttgcgatc gtcgccccgc cccatgcggg ctaaaagaat tcctgcaccc
     4321 accccttcat ggtccgcacc acgcgctgcc acgtcccgtt gccggaacgg gtcgtacgcg
     4381 acggctcacg atgtttccct ccgtacaaga tcaaccccac cccggtcgcg tacatggggc
     4441 tgttcacgat atccaccagc ccgccgacgt cggtgggagc gccccggcga accggcaggt
     4501 tcatcacgcg ctcggccagc tccggcatcc cgtcgaggat cgacgtgccg ccggtgatga
     4561 ccacgccggc ggccacgcga tcctcgtacc ccatcttgcg caattcctgc gccaccagcg
     4621 agaacatttc ttcggcccgc ggttcgatga tctcggacag gagttgacgc gacagcaccc
     4681 ggggcgggcg cccgcccatg ctgggcacct cgatggtttc gtggtcgcgc accatggacg
     4741 tggacgcgca cccgtagcgg atcttgatct tctcggcctc gtacgccggg gtgcgcagcc
     4801 ccaccgcgat gtcgttggtc acgtgattgc cgccgatgcc cagcaccgcg gtgtgccaca
     4861 ctccgttgtc cacgatcgtg gccaggtcgg tggtgccgcc cccgatgtcc accatcgcca
     4921 cgcccaactc acgctcctcg gcggtcaaca cggcctcgct ggaagccagc ggctgcaaaa
     4981 tcagatcgac gacctccagg cccgcgcggt tgatcgaccg cacgatgttc tgcgcggacg
     5041 tgaccgcgcc ggtgatgatg tgcacctcca cctcgaggcg cgtgccgaac atcccgatcg
     5101 ggtccttgat gccttcctgg tcgtccaccg cgaatccgcg cggcaacacg tggagaattt
     5161 cccgatccat cgggatcgcc accgccttgg cggcctccac cacgcgtttg acgtccgccg
     5221 cggtgacctc gcggttcttg atcgcgatca cgccgcggct gctgaagccc ttgatgtgcg
     5281 acccggcgat cccggtgtac accgagttga tctccacccc ggccatcaac tcggcctcct
     5341 ccacggcctt cttgatcgac tcgatcgtgc tctcgacgtt gaccaccacg cctttgcgca
     5401 ggccgcgcga cggatgggtg ccgatgccga tgatcttcac gtgcgggcgc tcgccgggga
     5461 tcgccccctc ggacacctca cccacaatcg cgcagatctt cgtcgtcccc acgtccaatc
     5521 cgacgacaat ctggtcgcgt ttcgccatgg cgctcctcct attccgcgtc cgccgatgaa
     5581 cgccgcggat ggccgctcat cgacggactc ctcggcgtgg atacgtcagc acgtccgccc
     5641 ttgccgcacg ttacagggcc cccgaccact cgctgaccac ggcccgatcc gagaaacgca
     5701 gatcaaccat tttcggtccc gtgatccgaa gcgccacgtt tccgcggatc gcttgcacgc
     5761 ggcgccactt ttcgatgaac ccctcgcgac cgaaccgcac gcgcatggcg ccgggcagtt
     5821 ccacgatcgg gtcatccggc gtcgagacgt cgatcaccaa cgactcgacc gcggcgatcc
     5881 gcacttcggc gacccgaaac gccgccagaa tctgcgcggc ggtcaccaac gcctccggcc
     5941 gcaccgccgc gcctccccgc acgaccggga acccccggtc gagcgcggtc ggcccgatca
     6001 cccggccgtc ggcgtccacc aagacgcggc cccgatcggt gacggccacc gcggcgggca
     6061 cccgctccgt cacggtgacg accaccgtat ccgggaaccg tttgccgacc gtcgcggcgc
     6121 gcacccatgg ccggccgtcg agccgtgcga ccacctcgtc gccgtccagc gtccacaacg
     6181 aggctcccat cggcaccgcg gcccacgcgc gcacctggcg ctcatcgagc cggaccgtcc
     6241 ccctgatctc cacgcgctcg atcacgtccc acccgcacaa cccggcaccc gtcccgcaga
     6301 ccgcgctcca cgaccacccc gccgcggtga ctccggtggc gcacaacacg acggcccaga
     6361 ggccccggcg aatccatcgc cgccgtttca cgttccgcgc tccagcgccg cgcgcacgat
     6421 cgcctccacc aggtcgtcga acgacaaccc caccccgcgc gcgatgtccg gcagcagact
     6481 ggtctcggtc atcccgggca aggtgttgac ctccagcacg tagggggtcc ccgcctgatc
     6541 caccaggcag tccacccggc cgtacccctc cgcgcgaagc gcccgaaacg cggccagccc
     6601 cacgtccagg gtccgccgca tcacgtccgg cggcagcgtg gccgggaaaa tgtgttcgga
     6661 catcccgggg gtgtatttcg cggtgtaatc gtaaaacgcg gtcttggatc ggatctcgat
     6721 cgcgcccagc gcccggtcgc cgagaatccc gacctgcact tccttgcccg cgatgtaggc
     6781 ctcgatcagc acgcgccggt cgtaccggaa cgcctccgcg atcgccgcgg gcaactcgtg
     6841 ggcgcccgac accagattga cgccgacgct cgagccctcg ctgttgggtt tgaccacgac
     6901 cgggaacgcg aacggaagcg acggcggagt cggctcggtg agcaggacgt atcggggaac
     6961 cggcaggccg gcggccgcga acaacgcccg ggaaatcagc ttgtccatgc ccagcgcgct
     7021 cgccagcacg cccgacccgg tgtagggaat ccccacgatc tccagcaaac cctggatcgt
     7081 cccgtcctcg ccgtaccggc cgtgcagcgc gatgaaggcc aggcccaccc gctcgcggat
     7141 caggcgatcg gccacgcccg cgtccacgtc gatcccgacc gcgtcaaaac cgcgtcgttg
     7201 caacgccgcc agcaccgcgg ctccggaccg aagcgacacc tcccgctccg cggaacggcc
     7261 gcccatcagc acaccgactc ggaccgcggt cgtcatgcgc gccccaccag tctgacctca
     7321 agctccaaag tcatgccccg ctcccgttgc accagcccgc ccacgtgacg gatcagcgcg
     7381 gcaagatcct tggccgtgcc ctcgccgacg ttcaccatga agttggcgtg ccgctcggac
     7441 acctgaatgc cgccgacccg gtagcccttg aggcccaatt catcgatgag ttgtccggcg
     7501 gaccggcccg gcggattctt gaaaatgcac cccgcgttgg ggaggttgag cggttgcgtg
     7561 cgccgccggt actcgagaat ggtttgcatc cgcgaatcga tcgcgtgggg atcgtccggc
     7621 ttcaatacca ccgtcgcgcc caccagcacg ccgggcggga tgtgcgactc ccgataccgc
     7681 aggcccagct tggacgcggc cagttccacc acctcgccct cccatgtcat caggcggacg
     7741 ctctccagca cgtcggcgat ctccccgtcg cgcgtgcccg cgttcatgac caccgcgccc
     7801 cccacggtcc cgggaatgcc gcaggcgaat tcgagtccgg acaatcccgc gtcctttgcc
     7861 gcgtgggaca gtgtcgggaa caatgcgccg gcctccgcgt agagtcggtt cacgcccgcg
     7921 ggacgaatcg atttgaggcc ggagagacgc agcaccaccc ccgccacccc tccatcccgc
     7981 accacgaggt tggtcccgcc caggaccatg accgggaccc gggccgcctt cgccgccacc
     8041 agcgccgacc gcagatccgc ttcatccgcc ggagtcacca gtgcatcggc cggcccgccg
     8101 accttcatcg acgtgtactt cgccaagggc tcgtcgaacc gcacctcccc ccgcacgccg
     8161 gaaagcgtgt cacgcaacga cggtcttacg gcaacggatg atcgtgtggt cacgggcacc
     8221 tctcccggca actgagggtt tgagcctgct caggatcggt cagatgcccc cgtcggggac
     8281 ttccgacatt ccacttcctg gcggagggtc gcagggcgcc gcgagcaccg gaggcgcagc
     8341 ggcaacgccg cagatggccg gtcatgagca ggctcacggc cgctccaaga acgcggggcc
     8401 gatcttccac acgtctcccg ctcccagcgt gatgaccatg tctcccgctt gcacgacctg
     8461 cgcgatccgc tccaccgccg cgtcgcggct ctccatccag gtcgcgtcgc ggtgcccgtg
     8521 ctcgcggatc gccgccacca acgcctgtga gttcacgccc tcgatgggcg actcgcccgc
     8581 cgcgtacacg tcgagcacca gcagcacgtc ggcctgataa aacgcggtgc agaaatccgc
     8641 gaagagatcg cgggttcggg aataccggtg gggctgaaac accacgacca gacgccgtcc
     8701 ccacccctcc ttggcggccg cgagcgtggc gcggatctcc gtggggtggt gcccgtaatc
     8761 atcgaccacc agcacgcctt ggcgctcccc ccgcatctgg aaccgccgct ccacgcccga
     8821 aaactgcgcc aggccgtcgc ggatcgcgtc aacgggcaga tccaactcca gccccaccag
     8881 gatcgcggcc atcgcgttcg agacgttgtg agcgccgggc accgtgaggt tgaagcgtcc
     8941 cagatccgac gcgcccagcc gcgcgtgaaa ttccgcgccc ttggggctga gacggatgga
     9001 cgtggccgtc aaatccgcct gcgggctccg gccgtaggtt cggacccgtt tttcgatctt
     9061 ggggatcaac gcctggaggt tcggttcgtc caaacacaat accgccgctc cgtagaacgg
     9121 caccttgttg acgaagtcca ggaacgcgcg cttgatcgcc tcgaggtctt ggtagtggtc
     9181 caggtgttcg cggtcgatgt tggtgaccac cgcgatggtg ggcgagagct tgaggaacga
     9241 cccgtcgctc tcgtcagcct ccgccaccag aaactccccc tgcccgagtt tcgcgtgccc
     9301 gccgaaacgg ttcagcttgc cgccgatcac cgcggtggga tccagcccgc cgtgcgcgag
     9361 cacggtggcg atcatggacg tcgtggtggt tttgccgtgc gcgcccgcca ccgcgatgcc
     9421 gtacttgagc cgcatcagtt cggccagcat ctccgcgcgg ggaatcacgg gaatcatgcg
     9481 cgcgcgcgcg gccgcgactt cgacgttggc gggcgacacc gcggacgagg tcaccaccac
     9541 ctgcgcgccc tcgacctgct cggcccggtg gccgatatac acggtcccgc ccaacgactc
     9601 caggcgacgg gtgacttcgg aggccgcaag gtccgagccg gtgacccgat acccgagcgt
     9661 gagcagcacc tccgcgatgc cgctcatgcc cgagccgccg atccccacga agtgcagccg
     9721 ttggatcttc ttaaacaccg atcatctcac cgctccacca gcgccagaca cgcggccgcg
     9781 atccgcgcac ccgcgtcggg tcgtcccagc gcgcgactcg cctccgccat gcgcagcagg
     9841 cgcggacggt ccgccagcag gtccgcgatc gcctcgccga gcatcgcgcc cgtcagatcc
     9901 cgttcaacga ccgtcaccgc ggcgcccgcc gcctccaccg cgcgggcgtt gcgctcctga
     9961 tggccgtgcg ccgcgtgcgg gtacgggatc aagatcgccg gccggccgca ggcggtcagc
    10021 tccgccacgg tggtcgcgcc ggcccgcgcc accaccagtg ccgcgcgcgc gtaggccgcg
    10081 cccatgtcgt ccacgtacgg ggtcaccgtg gccgtgactc cggccgcctc gtacgcctgt
    10141 ttggtctcgg cgaaatccgc ttgaccggtc tggtgcacga tcgacagccc cgtgattttc
    10201 gacagcgcgg gcaacgcgcc gacgaccgcg cgattgatcg cccgagcgcc ttggctccct
    10261 ccgaacacca ggatcgacgg cgcatccggc accgcggtcc gccccgcgga caccacggcc
    10321 gggcgcaccg ggttgcccaa cacctcgacc cggccgcgaa acgcggcccg cgactcctcg
    10381 aacgacgcca cgaccagatc cgcgaccggc gccagccacc ggttcgtcac gcccggcagc
    10441 acgttctgtt ccaggagcac gatggtgcgc cgcaacagcc gggccgcggc aatggtgggc
    10501 ccggctgcgt agccgcccac gcccaccacc agatccgggc gaaaccgtcg gagcacgcgc
    10561 accgcctcca gcagcgacac gggtagccgc gcgatcgtgg cggccgcgcg cacgacgcct
    10621 cggccgacca gtccccgcac ccggatcgtg gccaattcga atccttcgcg aggcaccacc
    10681 cgggactcca gtccgcgctc ggttccgaca aacagcacgg acgagccggg ccgcgcccgc
    10741 aagaacacgt gcgccagggc gatgcccgga tacaagtgcc cgccggttcc gccgcccgcg
    10801 atcaccaccc gcatgcgcga ctcaggttca acacgttcaa aggcttcaaa agttcaacgt
    10861 tcaacgttga accccttgaa ccttgaaccc ttgaacgttg gacctcttgg cccctctgaa
    10921 cgttgaacgt catttgatgc cccgctgccg cgagatgttg aacacgaccc cgagcgcgaa
    10981 caggttggcg accaacgacg atccgccgta gctgaccagc ggcaacggca accccttggt
    11041 cggcaacatg cccgtggcca ccccgatgtt gagggccgcg gccaatccca ccgccaccga
    11101 caaaccgaag gcgagcagac ggccgaacgg gtccgtggtc gccagcgcga cccgccaccc
    11161 gcgccacaac aggaccgcga acagcgccag aatcgccgcg gtgccccaca gccccaattc
    11221 ctcgcccacc accgagtaga taaaatcggt atgaggctcc ggcaggaaaa acagtttttg
    11281 ccgtccttcg cccagccccg cgcccatggt tccgcccgcc ccgaacgaca acatcgattg
    11341 aatcacctga aacccggagt cgctcgcgtc gctccacgga tcgagaaacg ccatgagcct
    11401 gcgtcgtcgg tacgcggtgc ccagaatcgc gtacgccacc acggggaccg cgcaggccgc
    11461 caacgccccg aggtggctca tccgcgcgcc gccgagcacg cacagcgccg cggtcacggt
    11521 cgccagcacc accgcgttcc cgaggtccgg ctcggccacg atcaacagga cgcccactcc
    11581 gacgaccacg agcaacgggg ccagggtctt gaacgaccgg atcacgtccg ctcgtttggc
    11641 cagcatgtgc gcggcgtaca cgatgaagat cacgcgcgcg aattcggacg gctggatcgt
    11701 gaacccgccc gcgcggagcc accggcgcga cccgttgacc gaccacccca tggtcaacac
    11761 gacggcgagg atgaccacgg ttcccaaggt gaggggcacc acgaaccgcc gccacacgtg
    11821 gtaatcgatc agcgcgccca gcccgaacgc ggcgagtccc accacggccc aggcggcctg
    11881 gcgcatcaca aaatacgtcg cgtcgtcgta ccgggtggtg gcgatcgcgg aactggcgct
    11941 gtagaccatc accagaccga tcacgacgag cacccccgcg gtcagggcga gcacgccgtc
    12001 tccccgcccc cactcgcggg cgtcgagccg cccgttcagc cacgcggacg cgggatgccg
    12061 ttgagcccgg gccctcacag cgcgttcacc agggttttga accgccgtcc ccgctcctca
    12121 aaatcgcgga agaggtcgaa actcgcacac gcgggcg
//
LOCUS       DBNGTT010000242         2253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2862157, whole genome shotgun sequence.
ACCESSION   DBNGTT010000242 DBNGTT010000000
VERSION     DBNGTT010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2862157"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(851..1333)
                     /locus_tag="AC16BC_05430"
     CDS             complement(851..1333)
                     /locus_tag="AC16BC_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241095.1"
                     /translation="MCVLRVTGRSFDVDLHLAQSGLNAYKVFRAGEPRFSSRPEGKRY
                     ELSGFTVDVRRHLPHDLPAQITDAVAFLKKYERDITHMRSAPGVDDMRLDFPVELRID
                     RRTVMAQFDYFPPQLVSLAGTLGLGLELSIYPPDLEAVARARSVDRQEPPTEATEDGP
                     "
     gene            1701..2090
                     /locus_tag="AC16BC_05435"
     CDS             1701..2090
                     /locus_tag="AC16BC_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3241096.1"
                     /translation="MVTVPSHPCDVDHLAHRVGCSRTHLIRSSKEAYGMPLGAYHTRC
                     RIRSAVAMLRRPEPNVGAVAFEVGYQSAKNARLARSGPASVTCASARSSITRSAAQAS
                     DRPSTPGARAGSRLRVPPVPGAPRTRR"
     gene            complement(1981..>2253)
                     /locus_tag="AC16BC_05440"
     CDS             complement(1981..>2253)
                     /locus_tag="AC16BC_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3241097.1"
                     /translation="RMALGAAPGRVRAMILRQVGVMTAIGGTIGLGAAIWAGTFARSI
                     LYEMESYDPMVIAASVALLALVALGAGFLPAHRASKVDPMLALRYE"
BASE COUNT          449 a          729 c          651 g          424 t
ORIGIN      
        1 tgtcggcggc agcgtggggc cccagacacc ccacgcggat ttctggcgcc tccgcgacgt
       61 tttgtcgact gacgtctgcg cggcctactc caagggtttt ctacgcgaac gggcctcatc
      121 aactctggtg agttcatgaa taatccgggt gaagacccgg ccctacatca gccctggaga
      181 cccggcaact ccatatccgt tcgtggcgcc ggctcctttt gtggcgccgg gtcttcagac
      241 ccggcggatt ttctactgca accaagccca cgtctgtcag tggtggaggg tggaattgtg
      301 gattaacact ggcggacgac caacgacgag ccaatcgacg gggaggcggc cgacagagca
      361 acctcggcgt cacctcgtcc agtacgaatg acgcattctc ccgggactat ccagcaccag
      421 agcgcaactt cgaatcgatg atgtccctca ctgcttggat cttctcaggc gactcttcat
      481 cgagtgcccg cctggcatcg aggccagaag gaccggtgca ctggagtaga agccggcacg
      541 cagaacgtca attcgtttca atctcggcaa ggttgtgtca actccaaacg caggtcccga
      601 cctaggggcg cctggggact gctcgcggat acccttccgg actggggctc ggtcgtggat
      661 tcggcggctg gccataccgg ccgccaggga tcacctcagg agagccgtgt cagcatcgcc
      721 gatagctctc gcctgcggca gcgatacact ccaaaagcca gccaccaggc agcgaccgga
      781 ggttcagaag gaaacgacac gttcgatcag tccgcgcaga gcgtccgtcg atttcgacgg
      841 tcacgtccgg ttacgggccg tcctctgtgg cctctgttgg cggctcttga cggtccacac
      901 tccgagctcg cgcgaccgcc tcaagatcag gcggatagat agacagttca agtcccaaac
      961 caagcgtacc cgcaagagag acaagttgag gcgggaaata atcgaactgc gccataactg
     1021 tcctgcggtc aatacgcagc tccactggaa aatccaagcg catgtcgtcc acaccaggag
     1081 ccgaacgcat gtgtgtaatg tccctttcgt atttcttgag gaacgctacc gcatccgtta
     1141 tttgggcagg cagatcgtgc gggagatgcc gtctaacgtc gacggtaaat ccagagagct
     1201 cgtagcgctt cccttccggc cgcgacgaaa aacgcggctc accagcccga aagaccttgt
     1261 atgcattaag tccggattgt gcaaggtgca ggtccacatc aaaagatctt cctgtgacgc
     1321 ggagtacgca catgtaattc tcctgaagtt gtccctaaag agcttccagc gtgattactt
     1381 aaaccacgtg cgattccaag catatccctc cgcgcagtcg tccatcatca gaatgcgaca
     1441 gcaacaacta ctactccacc tgaacctccc gacagtatca ccacggttcc agcgacgacc
     1501 actacgccgg ttgtgatcag gatccccatg ggtgtcttga tcgtcgtcgg gatccagtgt
     1561 tcaggggcac cgtagccgta accgatgttg ccgtggccgc cgacggcgtg gtgaagctgc
     1621 tgttgtatcg ggtcgtgttg gccgccgcca cggtctgaaa gacgacgggg acagtgacct
     1681 cggcatcgac caccgtgctc gtggtcaccg ttcccagcca tccctgcgac gtcgaccacc
     1741 tcgcccaccg cgttggctgc tcgcgcaccc acctgatccg atcttccaaa gaggcgtacg
     1801 gcatgcccct cggcgcctat cacacacggt gccgtatcag gtccgccgtc gcgatgttga
     1861 ggcggccgga gccgaacgtc ggagccgttg cgttcgaggt tggctatcag agcgcgaaga
     1921 acgctcgtct tgcccgctcc gggcctgctt cggtcacgtg cgcgtccgcg cgatcctcga
     1981 tcactcgtag cgcagcgcaa gcatcggatc gaccttcgac gcccggtgcg cgggcaggaa
     2041 gccggctccg agtgccacca gtgccaggag cgccacggac gcggcgatga ccatgggatc
     2101 gtagctctcc atctcgtaga gaatcgacct ggcgaacgtt cccgcccaga tggcggctcc
     2161 caggccgatc gtcccgccga tcgcggtcat cacaccaacc tgccgcagga tcatcgcgcg
     2221 cacccgtccg ggcgccgcgc cgagcgccat gcg
//
LOCUS       DBNGTT010000243         4018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1841454, whole genome shotgun sequence.
ACCESSION   DBNGTT010000243 DBNGTT010000000
VERSION     DBNGTT010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1841454"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="AC16BC_05445"
     CDS             complement(<1..222)
                     /locus_tag="AC16BC_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3241098.1"
                     /translation="MGERIANLINGRWQPAASGKTLESRNPADTNEVVAMAPASSSDD
                     VNQAVAAARAAFPVWRGVPAPKRGEILFRA"
     gene            338..2053
                     /gene="recJ"
                     /locus_tag="AC16BC_05450"
     CDS             338..2053
                     /gene="recJ"
                     /locus_tag="AC16BC_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR3241099.1"
                     /translation="MRKRWEQRPYNPDVVAALVQGLGVRRTTARILASRKVDSVEAAR
                     RFLAPSAADLHDPFLLTGMDRAVERIERAVAGRERILIAGDYDVDGVTATSLYLELFA
                     WLGTPVAYRIPHRLTEGYGLTETGVRAARDQGVSLLITADCGTTAHGPIELANSFGID
                     VIVTDHHEPQDRIPPAYALLNPHLPDSRYPEKVLCAAGIAFKVAQALLGRRRSLPGVD
                     GRLASVLDLVALGTVADVAPLRGENRYLVAEGLRALSAGRRVGVAALKTVCGMEGKRV
                     DAGMVGFQLGPRINAVGRLAAAHSGVTLLTTTDPQVAITAARELDAANQERRRIEEAM
                     LEGAVAKAERELSMDRARSIVLADEAWHPGVIGIIASRLVDRWHRPAIVIALRPDGKG
                     KGSGRSIPGFHLTRALEECREFFEGFGGHAAAAGLTIRTERIPTFRERFDAVVAATLS
                     TDSAQPILSLDDEVELVDVTFPLIDELSRLAPFGMGNPEPLLTSRGCAPRGARIVGNN
                     HLKLLVRHANAASLDAIGFGLGHLLPTDPGPIDLAYSARVDSWQGQDRIQLRLKDLRP
                     SQSGS"
     gene            2158..>4018
                     /locus_tag="AC16BC_05455"
     CDS             2158..>4018
                     /locus_tag="AC16BC_05455"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3241100.1"
                     /translation="MLDPVLAPKPAESTTASIDDIIAKVTAYNPQAPVDVLRRAYEVS
                     AKAHDGQMRKSGEPYVMHPLAVAEILTALRMDVPSIVAGLLHDTVEDTLMPRETIEQQ
                     FGADVMKLVDGVTKIGKIRFKSYEEKQAENFRKMILSMAEDIRVVLIKLADRLHNMRT
                     LSVLRPDKQQRIARETIEIYAPLANRLGIGWMKNELEDLCFKYLKSDAHQDLVGKVAK
                     QLAERQRYVDSVIEKVKAAMAEYGFKGTVYGRPKHLYGIYSKMERREIAFEEVYDLIG
                     IRIITDTKIACYGILGMIHSLWKPVPGRFKDYIGVPKSNLYQSLHTTVIGPQGEHVEF
                     QIRTEEMHRIAEEGIAAHWRYKDQGQIDEKDNRLFAWLRQLVEWQKDLTDNRQFLNSI
                     KMDLFTDVIYVFTPKGDVIELVKGATPIDLAYAVHTEVGHHCTGAKINGKLAPLKTVI
                     KSGDTVEIVTSPNQVPSKDWLKIAKTPKAKTRIKHWIKLEEHKQSLEIGRKLLERELR
                     RNHLSPNEVFKSDTLNAIASDSGLASVDDLLVALSYGRVSINHVLNRLLPESALKEGL
                     TDKLLTSIGKQPRGVTIKGINDIMINIAKCCNPVPGDKILGFITRGRGLSIHTA"
BASE COUNT          761 a         1372 c         1273 g          612 t
ORIGIN      
        1 cgcgcgaaac aggatctccc cgcgcttggg ggcggggacg ccgcgccaca cgggaaacgc
       61 ggccctggcc gcggccacgg cttgattcac gtcgtcggac gacgacgcgg gcgccatggc
      121 cacgacctcg ttggtgtcgg ccgggttccg gctctccagc gttttgccgg aggccgcggg
      181 ttgccagcgg ccgttgatca ggttcgcgat gcgttcgccc atcacccgcc tccagcgttc
      241 gccctcggtg tatcagaaac cctccgggcg cgcaagccgt tgacaccgcc ggtcgcctgc
      301 gcgtatggtg acccgcaacg ctgtcaggga cgaccgtatg cgcaagcgct gggagcagcg
      361 cccctacaac ccggacgtgg tcgcggcgct cgtccagggt ctcggtgttc gccgcaccac
      421 cgcgcgcatc ctcgcaagcc gcaaggtcga ttcggtcgag gccgcgcgcc ggtttctcgc
      481 gccgtccgcc gcggatctgc acgacccgtt cctgctgacc ggcatggatc gcgcggtgga
      541 gcgcatcgag cgcgccgtcg ccggccgcga acgcatcctg atcgcgggcg actacgacgt
      601 ggacggggtg accgccacct cgttgtacct ggaactgttc gcctggctcg gcacgccggt
      661 cgcgtaccgg atcccccacc gcctcaccga agggtacggc ctgaccgaaa ccggcgtgcg
      721 cgcggcgcgc gaccagggcg tctcgctcct catcaccgcg gattgcggca ccaccgcgca
      781 cggcccgatc gaactggcca acagcttcgg catcgacgtg atcgtcacgg accaccacga
      841 gccgcaggat cggattccgc ccgcgtacgc gttgctcaac ccccatctgc ccgactccag
      901 ataccccgag aaagtgttgt gcgcggccgg gatcgcgttc aaggtcgcgc aggccctgct
      961 cggccggcga cggagcctgc cgggcgtgga cggccgcctc gcgtccgtgc tcgacctggt
     1021 ggccctgggc acggtcgcgg acgtggcgcc gttgcgcggc gagaaccgct acctggtggc
     1081 cgagggattg cgcgcgttga gcgcgggccg ccgcgtgggc gtggccgcgc tcaagaccgt
     1141 gtgcggcatg gagggcaaac gcgtggacgc gggcatggtg ggctttcaac tcggcccgcg
     1201 catcaacgcg gtgggccggc tcgccgcggc ccattccggc gtgacgctgc tgaccacgac
     1261 cgatccgcag gtcgcgatca cggccgcgcg cgaactggac gcggcgaatc aggagcgacg
     1321 gcggatcgag gaagccatgc tggagggcgc cgtggccaag gcggaacgcg aactctccat
     1381 ggaccgcgcg cgttcgatcg tgctggccga cgaggcctgg caccccggcg tgatcgggat
     1441 catcgcctcg cgcctggtgg accgttggca ccgccccgcg atcgtgatcg cgctgaggcc
     1501 tgacggcaag ggcaagggct cgggccgcag catcccgggg ttccatctga cccgcgccct
     1561 ggaggaatgc cgggaattct tcgaaggctt cggcgggcac gcggcagcgg ccggcctcac
     1621 gattcgaacc gaacgcattc ccacgttccg cgagcgattc gacgccgtgg tagccgccac
     1681 gctgtcgacg gattccgccc agccgatcct gtcgctggac gacgaggtgg aactggtcga
     1741 cgtcacgttc cccttgatcg acgaactctc gcgcctggcg cccttcggaa tgggcaaccc
     1801 ggaacccttg ctgaccagcc gcgggtgcgc gccgcgcggc gcgcggatcg tgggcaacaa
     1861 ccacctcaag ctgctggtcc gccacgccaa cgccgcgagc ctcgacgcga tcggcttcgg
     1921 cctgggacac ctcctgccca ctgatccggg cccgatcgac ctggcctact cggcccgcgt
     1981 ggactcctgg caaggccagg accgcatcca attgcgcctg aaagatctgc gccccagcca
     2041 atccggctcg taggacccgc accgtagggg ttcggcatgc cgaaccccta ccagccgatc
     2101 gcccctcgcc gcgtcggcat cgactcagcc ccggacgatc tatataatga aggtatgatg
     2161 ttggatcccg tactggcccc caaacccgct gaatcgacca ccgcgtcgat cgacgacatc
     2221 atcgccaagg tcacggccta caatccgcag gcgccggtcg acgtgttgcg gcgcgcgtac
     2281 gaggtctcgg ccaaggcgca cgacggccag atgcgaaaat ccggcgagcc gtacgtgatg
     2341 cacccgctcg cggtcgcgga gattctgacc gcgctccgga tggacgtgcc ctcgatcgtc
     2401 gcgggcctgc tccacgacac ggttgaagac accttgatgc cgcgcgaaac catcgagcag
     2461 cagttcggcg ccgacgtgat gaaactcgtg gacggcgtca ccaagatcgg caagatccgg
     2521 ttcaagagct acgaagaaaa gcaggcggaa aatttccgca agatgatcct gtccatggcc
     2581 gaggacatcc gggtggtcct catcaagctg gccgaccgcc tgcacaacat gcgcaccttg
     2641 agcgtgctgc gcccggacaa gcaacagcgc atcgcgcgcg aaaccatcga aatctacgcg
     2701 cccctggcca accggctcgg catcggctgg atgaaaaacg agttggaaga tctgtgcttc
     2761 aagtacctga agtccgacgc gcaccaagat ttggtgggta aggtcgccaa acagctcgcg
     2821 gaacggcagc gctacgtgga ctcggtcatc gagaaggtca aagccgcgat ggccgaatac
     2881 gggttcaaag gcacggtcta cggccggccc aagcacctgt acggcattta ctccaagatg
     2941 gagcgccgcg agatcgcgtt cgaggaagtc tacgacctca tcggcatccg catcatcacc
     3001 gacaccaaga tcgcctgtta cggcatcctg ggcatgatcc actcgctctg gaaacccgtt
     3061 ccggggcgct tcaaggatta catcggcgtt cccaagtcca acctctatca atccttgcac
     3121 accacggtga tcggcccgca gggcgaacac gtggagttcc agatccgcac cgaagaaatg
     3181 caccgcatcg cggaagaagg gatcgcggcg cactggcggt acaaggacca gggacagatc
     3241 gacgaaaagg acaaccggct gttcgcgtgg ttgcgccagc tcgtggagtg gcagaaggat
     3301 ttgaccgaca accgccagtt cctcaactcg atcaagatgg acttgttcac cgacgtgatc
     3361 tacgtgttca cgcccaaggg cgacgtgatc gagttggtga agggcgccac gccgatcgac
     3421 ctggcctacg cggtgcacac cgaggtgggc caccactgca ccggcgccaa gatcaacggc
     3481 aaactcgccc cgctcaaaac cgtgatcaag agcggcgaca cggtggaaat cgtcacctcg
     3541 cccaaccagg tcccgtcaaa agactggctc aagatcgcca agacgcccaa ggccaagacc
     3601 cgcatcaagc actggatcaa actggaagaa cacaaacaaa gtctggaaat cggccggaag
     3661 ctgttggagc gtgaactgcg acgcaaccac ctcagcccca acgaagtgtt caagtccgac
     3721 accctcaacg cgatcgcgtc cgacagcgga ttggcctcgg tggacgattt gctggtggcg
     3781 ctcagttacg gccgggtgtc gatcaatcac gtgctcaacc gtctgctgcc ggaatccgcg
     3841 ctcaaggaag ggctcaccga caagctcctg acgtcgatcg gcaaacagcc gcgcggggtc
     3901 acgatcaagg gcatcaacga catcatgatc aacatcgcca agtgctgcaa tccggtgccg
     3961 ggcgacaaga tcctggggtt catcacgcgc ggccgcggcc tgtccatcca caccgcgg
//
LOCUS       DBNGTT010000244         5733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3247089, whole genome shotgun sequence.
ACCESSION   DBNGTT010000244 DBNGTT010000000
VERSION     DBNGTT010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3247089"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="AC16BC_05460"
     CDS             complement(<1..205)
                     /locus_tag="AC16BC_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241101.1"
                     /translation="MKSALRRGLVGSAGLVLIAALSWPVYVAMSNTNLAKGTAVDGKK
                     HVMLVGASVGKAWKLADFTRRTND"
     gene            complement(202..807)
                     /locus_tag="AC16BC_05465"
     CDS             complement(202..807)
                     /locus_tag="AC16BC_05465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3241102.1"
                     /translation="MKTLRALVSRLKRAYRLRGYNRFTIAELFREQGAQIGEGCSIIP
                     TSLGMEPYLIKIGNHVTIAGGVYFMTHDGGVWIFRDECPDLQVFGPIVIEDNCVIGQN
                     AILFPNIKIGPNAIVGAGSVVISDVPPDTIVMGIPARPFGSVAKYREKCLERWAEQRP
                     PDLQIESGRDWWNSEHFEANRRKLKKHLQKVFHVQLKGAAS"
     gene            complement(804..2444)
                     /gene="xrtW"
                     /locus_tag="AC16BC_05470"
     CDS             complement(804..2444)
                     /gene="xrtW"
                     /locus_tag="AC16BC_05470"
                     /inference="COORDINATES: protein motif:HMM:NF038142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase W"
                     /protein_id="HIR3241103.1"
                     /translation="MRSADSEHQEPLVLMARSAPAALGKAWWPAAALVFGVAVIYGPT
                     IASLARMWWSSDDYSHGILVPFISLYFVWMKRDVIARTDATPSVWLGVPVIAAAGLLL
                     HAGKIGGVMVLQEISLLVMIAGLVLTLLGPAMLRVLAVPIAYLVLMIKIFGEGGDRFH
                     WPFQILAADIGVLLLKIFGYPVFQDAQYIHLPRITLEVAAACSGLRFLVSIIAIGIPL
                     AYLTQRTWFAKVGLVSFGIAVAVLANGLRVALIGVWTSNGGDLVHGPFHLLSGVFVSW
                     VGFVALFAGVWLFRRLSPGVHGVDRGQADFSAGVHAAIPGSGRRAAWAVTAALLLGIG
                     VSYYAAWVRPVSLAAGLDALPATINGWRGEERPVDDQPWRIAGADDELLRIYRDSEGR
                     SVRLYIAYFRSQEQGHDLVNYKTSRLLHRDERTIPIAAKTGDTEYVNRADLNSPAGRS
                     PVLFWYVIGGRGTPDSYRAKLFTIWDGIAARRTSGAVVVVSSPPERPGDAQRAADAQR
                     FAEDAWGAIQGHLSASSSRQSNRMAGAQPTLNDGEGVQ"
     gene            complement(2428..3417)
                     /gene="nadE"
                     /locus_tag="AC16BC_05475"
     CDS             complement(2428..3417)
                     /gene="nadE"
                     /locus_tag="AC16BC_05475"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR3241104.1"
                     /translation="MPQPFSKDVLTIDPEPTVRRIAEAIRTQVGATLKRRGVVLGLSG
                     GIDSSVTAALCANALGPERVLGLLMPEQDSSDDSLRLGRLLAEHFRIPTVVEAIGPLL
                     DASGCYRRRDEAIRAQVPQYDKGYRCKIVLANILEHEGYNFFRLVVESDRGERTTVRL
                     SAQTYLAIVAATNMKQRARKFFEYYHADRLQYAVAGTPNLLEYDQGFFVKNGDGAADI
                     KPIAHLYKSQVYQLAAYLGVPGEIRQRPPTTDTYSLAQSQEEFYFSLPYDKMDLCLYG
                     KNHQVPPADVAKVIGLTAEQVGRVYRDIDVKRSATRYMHLKPLLVEPVDEVGG"
     gene            complement(3432..4937)
                     /locus_tag="AC16BC_05480"
     CDS             complement(3432..4937)
                     /locus_tag="AC16BC_05480"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3241105.1"
                     /translation="MRIDQAIAETIARQPDRAAAAFRGSRLTYGELGSRADDLRRRLA
                     DAGLSPGDRVVLWLENSPEYIASYLAVLGAGAVVVGMHPQAHPSEVVRVIEHAGASGL
                     IFSASNPRTDVRGVDDAGLVWRFDGREVTAGSSPRRPGAGPDGLAQIIYTSGSTGRPK
                     GVMLSHRNLLANTRSILEYLALTADDRIVAVLPFVYSYGNSVMLTHLAVGGLLVLENS
                     FLYPNVILDRMVEHAVTGFSGVASTYALLVDQSNLAAYRFPALRYLTHAGGPMPSGLL
                     ARARSAFAGKDVYVMYGQTEASARLTYLPPGQLDAKAGSAGRAIPGVTIRVVKEGGEP
                     AKAGEVGEVLAAGDNIMMGYWGDPEGTGRALSEGWLHTGDIGFLDADGYLTLVGRNSE
                     IIKSGAFRVSPTEIEDVLLAHPGVSEAGVVGIDDAVLGEAICGVVVPRAGRRPTNQEL
                     LAYCAKHLAPYKRPKVICQTDALPKSPSGKVLRHGLREIGRAATHNAGGPG"
     gene            complement(4937..5203)
                     /locus_tag="AC16BC_05485"
     CDS             complement(4937..5203)
                     /locus_tag="AC16BC_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3241106.1"
                     /translation="MDTQIEQQVRDFIMTNFLFGDTSKKLASDDSFLEKGIIDSTGVV
                     QLVTFVEETFGIAVDDAELVPENLDSVEKLVRFIERKRSAASTH"
     gene            complement(5245..>5733)
                     /locus_tag="AC16BC_05490"
     CDS             complement(5245..>5733)
                     /locus_tag="AC16BC_05490"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3241107.1"
                     /translation="RGYWERPEETARALRPGPHVDEKVLYTGDLFTMDDEGYLYFVGR
                     RDDVIKTRGEKVSPKEVEAVLYSLPAVAEAAVVGVPDDVLGQAVKAVIVLASGARCTE
                     QDILRHCAEHLEGFMVPRSVEFCEILPKTLTGKIETRSLGGRMMPDGDAHGLDRMEAS
                     RP"
BASE COUNT         1106 a         1977 c         1652 g          998 t
ORIGIN      
        1 catcgttggt gcgacgcgtg aaatcagcca acttccaggc tttgccgacc gacgcaccca
       61 ccagcatgac gtgcttcttt ccatccaccg ccgtcccctt tgcgagattt gtgttggaca
      121 tggcgacata gacgggccac gacaaggccg cgatcaatac cagtcccgcc gatcccacca
      181 gccccctcct gagtgcgctc ttcatgacgc ggctcccttc aactggacgt gaaagacttt
      241 ctgcagatgt tttttgagct tcctgcgatt ggcctcgaag tgttcggagt tccaccaatc
      301 gcggcccgat tcaatctgta agtcgggtgg gcgctgttcc gcccagcgct cgaggcattt
      361 ctcccggtac ttggccactg acccgaacgg ccgcgccggg atgcccatca cgatcgtatc
      421 cggaggcacg tccgagatca cgaccgaccc ggcgcccaca atggcgttgg gaccgatctt
      481 gatgttggga aacaagatcg cgttctgccc gatcacgcaa ttgtcctcga tcacgatcgg
      541 cccgaagact tgcaggtccg ggcattcgtc gcgaaagatc cagacgccgc cgtcatgggt
      601 cataaaatac acgccccccg cgatcgtcac gtggttgccg attttgatta aatacggctc
      661 cattccgagc gacgtcggga tgatgctgca cccctctccg atctgcgcgc cctgttcgcg
      721 gaaaagctcc gcgatcgtaa agcggttata accccgcaac cggtacgctc gtttgagccg
      781 tgacaccaac gcacgcagcg tcttcactgc acaccctccc catcattcaa tgtcggctgc
      841 gcccccgcca tacggttgga ttggcgggat gacgaggccg acagatgccc ctggatcgcc
      901 ccccaggcgt cctcggcaaa ccgctgcgca tcggctgccc tttgcgcatc acccggccgt
      961 tccggagggg acgacaccac gaccacggcg ccgctcgtgc gtcgagccgc aatcccgtcc
     1021 cagatcgtga acagtttggc ccgataactg tccggcgtcc cgcgtccgcc aatgacatac
     1081 caaaacagca ccggagaccg gccggcgggt gaattcaagt cggcgcgatt cacgtattcg
     1141 gtgtccccgg tcttggccgc aatcgggatc gtccgttcgt cgcggtgcag caaccgcgac
     1201 gtcttgtagt tcaccaaatc gtgcccctgc tcctgggaac gaaaatacgc gatatagagc
     1261 ctcacggatc gaccctcgga gtctcgatag atccggagaa gttcgtcatc ggcccccgcg
     1321 atccgccacg gctggtcgtc cacgggtcgt tcctcacccc gccacccgtt gatcgtcgcg
     1381 ggcagggcat caagtcccgc ggccagcgac accggcctga cccacgccgc ataatacgag
     1441 acgccgattc cgagcaggag cgccgcggtc acggcccacg ccgctctccg tcccgagccg
     1501 ggaatcgcgg cgtggacgcc ggctgaaaag tcggcttggc cacggtctac accatggacg
     1561 cccgggctca accgccgaaa aagccacacg ccggcaaaca acgcgacgaa tccgacccac
     1621 gagacaaaca cgccggacaa gagatgaaac ggtccgtgga ccagatcccc gccgttggac
     1681 gtccagactc cgatcagcgc cacgcgcaac ccgttggcca ataccgccac cgcgataccg
     1741 aacgacacca gcccgacctt ggcgaaccac gtgcgttggg tgaggtaggc cagcggaatg
     1801 ccgatcgcga tgatggagac caagaaccgc agcccgctgc acgcggccgc gacctccaag
     1861 gtgatgcgtg gcaggtggat atactgggcg tcctgaaaaa ccggataccc gaagattttg
     1921 agcaacaata cgccgatatc cgcggccagt atttgaaacg gccagtggaa gcggtctccg
     1981 ccctcgccga agatcttgat catcaacacc agatacgcga tcggcaccgc gagaacccgg
     2041 agcatcgccg ggcccaggag ggtcaacacg agccccgcga tcatcaccag caacgagatc
     2101 tcctggagca ccatgacccc gccgatcttt cccgcgtgca ggagcaggcc ggccgcggca
     2161 atgacgggca ccccgagcca gaccgacggc gtggcgtcgg tgcgcgcgat gacgtcgcgt
     2221 ttcatccaca caaagtagag ggagatgaaa ggaaccagga tgccgtggct gtaatcatcg
     2281 ctgctccacc acattcgcgc cagtgaggcg atggtggggc cgtagatgac ggccacgccg
     2341 aacacgagcg cggccgcggg ccaccaggct ttcccgagcg ccgcgggtgc agaccgcgcc
     2401 atcaacacga gcggctcctg atgctcgcta tccgccgacc tcatccacgg gctccaccag
     2461 gagcggtttg aggtgcatgt agcgcgtggc gctgcgcttg acgtcgatat cccggtatac
     2521 ccgcccaact tgctccgcgg tcaggccgat cactttggcc acatcggccg ggggcacctg
     2581 atgatttttc ccatacaggc agaggtccat cttgtcatac ggcaacgaga agtagaactc
     2641 ttcctgggac tgcgccaggg agtatgtgtc ggtggtcggg gggcgctgac gaatttcacc
     2701 cggaacaccc aaataggccg ccaattgata gacctgggac ttgtacaggt gcgcgatggg
     2761 tttgatgtcc gcggcgccgt ctccgttctt cacgaagaaa ccctggtcgt attcaagcag
     2821 attcggcgtg ccggccaccg cgtactgcag acggtccgca tggtagtact cgaaaaattt
     2881 tctggcgcgt tgtttcatat tcgtggcggc tacaatggcg aggtaggtct gcgcgctgag
     2941 ccgcaccgtg gttcgttctc cccggtccga ttccaccacc aaccggaaaa agttatagcc
     3001 ctcgtgctcc aggatattcg ccagcacgat cttgcaccgg tagcccttgt cgtactgggg
     3061 gacctgagcc cgaatcgctt cgtcgcgccg gcgataacat ccggacgcgt ccagcagcgg
     3121 gccgatggcc tcgaccaccg tgggaatccg aaaatgttcg gccaacagcc gccccaggcg
     3181 caggctgtcg tcggatgagt cctgttccgg catcaggaga cccagcaccc gctcgggccc
     3241 cagtgcgttc gcgcacaagg ccgcggtcac gctgctgtcg atgccgcccg acaacccgag
     3301 cacgaccccg cggcgcttga gcgtggcgcc cacctgcgtc cgaatcgctt ccgcgatccg
     3361 acgaaccgtg ggctcgggat caatggtgag gacgtctttt gaaaatggtt ggggcattgc
     3421 tctccctctg gttagccggg gccgccggca ttgtgcgtgg cggcacgacc gatctccctc
     3481 aagccgtgac gcaggacttt tcccgacggc gacttcggca gcgcatcggt ttggcaaatg
     3541 actttgggcc gcttgtacgg cgccaggtgt ttggcgcagt aagccaacaa ctcttggttg
     3601 gtcggcctgc gtccggcccg cggcaccacg accccgcaga tcgcctcacc caacaccgcg
     3661 tcgtcgatcc cgaccacccc ggcttccgac accccgggat gagcgagcaa cacgtcttcg
     3721 atctcggtgg gactgacgcg aaacgccccg gacttgatga tctcgctgtt gcgtccgacc
     3781 aacgtgaggt atccgtccgc gtccagaaac ccgatgtccc cggtgtgcag ccagccctcg
     3841 gagagcgcgc ggcccgtgcc ttcgggatcg ccccaatacc ccatcatgat gttgtcgccg
     3901 gcggccagga cctcgccgac ttccccggcc ttcgcgggct ccccgccctc cttgaccacc
     3961 cgaatcgtca ccccggggat ggcccgcccc gcggagcccg ccttggcgtc gagttgcccg
     4021 ggcgggagat acgtcaggcg cgccgaggct tcggtctgcc cgtacatgac gtagacgtcc
     4081 ttgccggcga aggcggaacg cgcgcgcgcc aacaggcccg acggcatggg gcccccggca
     4141 tgggtcaaat accgcaacgc gggaaaccgg tacgccgcga gattggattg atccaccagc
     4201 aaggcatagg tcgatgccac tcccgaaaat ccggtcaccg cgtgctccac catgcgatcg
     4261 aggatcacgt tcgggtacag aaaactattt tccagcacga gcaatccgcc gacggccagg
     4321 tgcgtcaaca tgaccgagtt gccgtacgag tacacgaacg gcaacaccgc cacgatccgg
     4381 tcgtcggccg tcagtgccag atactccaga atcgatcggg tattcgccaa caggttgcga
     4441 tgcgacagca tcaccccctt gggccgcccc gtggaccccg aggtatagat gatttgcgcc
     4501 aagccgtcgg gccctgcgcc cggacgcctt ggcgaagacc ccgccgtcac ctcccgcccg
     4561 tcgaaccgcc agaccaaccc cgcgtcgtcc actccccgca catcggttcg gggatttgac
     4621 gccgagaaga tcagtccgga ggcacccgcg tgttcgatca cgcgaacgac ttccgagggg
     4681 tgggcctgag gatgcatgcc gaccaccacc gcccccgcgc ctaggaccgc caggtatgac
     4741 gcgatatact cgggtgaatt ctccaaccag agaaccaccc ggtcccccgg ggacaacccg
     4801 gcgtcggcca aacgacgccg caaatcgtcg gcgcggcttc ccagctcgcc gtacgtcagt
     4861 cgggaaccgc ggaacgccgc cgccgcgcga tccggctgac gggcgatggt ctccgcaatg
     4921 gcctggtcga tcctcatcaa tgcgtcgacg ccgcgctccg cttccgctcg ataaaccgga
     4981 cgagtttctc gaccgaatcg aggttctccg ggacgagttc cgcgtcgtcc acggcaatgc
     5041 cgaacgtctc ctcgacgaac gtcaccaatt ggacgacgcc cgtcgaatcg atgatgcctt
     5101 tttccaagaa cgaatcgtcg gacgccaact tcttggaagt atcgccgaat aagaaattcg
     5161 tcatgatgaa atcccgaacc tgctgctcga tctgcgtgtc cacgttcact ctcccaccat
     5221 caatagtcgg tcccgatcgg ctgttcacgg ccgcgacgcc tccatccgat ccaggccatg
     5281 ggcatcccca tccggcatca tgcgcccacc gaggctccgg gtctcgatct tgccggtcag
     5341 ggtcttcggc aagatctcgc aaaactccac ggaacgcgga accataaatc cttccaggtg
     5401 ctcggcgcag tgacgcaaaa tgtcctgttc ggtgcagcgg gcccccgagg cgaggacgat
     5461 caccgccttg acggcctgcc ccaacacgtc gtcgggcacg cccaccaccg cggcttccgc
     5521 caccgccggg aggctgtaca acacggcctc cacttctttg ggactgacct tttcgcctcg
     5581 cgttttaatc acgtcgtccc tgcgtccgac gaaatagaga tacccctcgt cgtccatggt
     5641 gaacaaatcg ccggtataca gtaccttttc gtccacatgt ggaccgggac gcaacgcgcg
     5701 cgcggtctct tcggggcgtt cccagtaccc ccg
//
LOCUS       DBNGTT010000245         6889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3119501, whole genome shotgun sequence.
ACCESSION   DBNGTT010000245 DBNGTT010000000
VERSION     DBNGTT010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3119501"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="AC16BC_05495"
     CDS             <1..736
                     /locus_tag="AC16BC_05495"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3241108.1"
                     /translation="GIEVDTLALMTDVVRELLPAGSRHPVAVLSGPSFAAEVAKGHPT
                     AVTLACRDPHAARRLQELFTTATFKLFTTPDVIGVQVGGALKNVIALAAGGSDGLGFG
                     ANTRAALITRGLAEISRLGVAMGGQPWTFSGLSGLGDLVLTCTDQQSRNFTVGYRIGR
                     GETLETITRGASTVAEGVRTARAAYALSRKYRVLMPIVEQIYAVLYEGKDPRKAVTDL
                     MEPAVGDELFRPQERGGGWGDPADAR"
     gene            733..2361
                     /locus_tag="AC16BC_05500"
     CDS             733..2361
                     /locus_tag="AC16BC_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR3241109.1"
                     /translation="MNDIERVLLELAGGVSLLRYGLHLAGEGLQALAGARLRYALASV
                     TKNRVLGLGAGALITAILQSSSATTVMLVGFTGSGLLTLRQAMAVILGADVGTTATVQ
                     LLAFPVSEYALGVIAIGFLLFFFGERQRTKTLGTALLGFGLIFLGLRLIASGAEPLVD
                     SPLVGQAFMALGDHPVLGIAVSATVTAMLHSSAATIGIALALSAHGLLTLHGALPIIL
                     GANVGTCAPALISSLGGVPEAKRVALAHALFKVAGVVLVYPVLPLFETWVEASAVSLA
                     HRIANAHTLFNLGLAVAFLPFEGPFAGVVARLVKERPRADEWARPKYLDPHVLDVPSL
                     ALSQATRETLRMADLVHEMVRDTIKAFADGDQELVESIERKEDWVDALNREIKLYITK
                     LSEKSLTKEQLDREMVLLAVINDLENIGDIVDKNLMELAKKKLYKDLRFSENGVREIV
                     ELHTLVERNFERVIAAFASQDAEVAKQVIEEKARISYKERQLKQAHIHRLHAGLPESI
                     ETSAIHLDVLTNLKRINSHVTNIAYPLVDQPSEG"
     gene            2361..3170
                     /gene="larB"
                     /locus_tag="AC16BC_05505"
     CDS             2361..3170
                     /gene="larB"
                     /locus_tag="AC16BC_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR3241110.1"
                     /translation="MDRGRLRALLEAVRDGASDVDGALAALRDFPSEALGFAALDHHR
                     DLRQGVPEAVLCEGKTAAEAAAIVKRLAVSGAPVIATRAEPAVARAIRKVAPDATYHP
                     RARVCILHPRSAFDPHGAVVVTAGTADLPVAEEAALTAGALGTGVSLVCDVGVAGVHR
                     LVDQLDRLRAARVVIVVAGMDGALPSLVGGLMAQPVIAVPTSRGYGAHFGGIAPLLTM
                     LNSCAAGLAVVNIDNGFGAGVLAHRIERLGRQPAGDNGSPNGRRPRRSRHG"
     gene            3174..4571
                     /gene="larC"
                     /locus_tag="AC16BC_05510"
     CDS             3174..4571
                     /gene="larC"
                     /locus_tag="AC16BC_05510"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR3241111.1"
                     /translation="MKIAYFDCFSGISGDMVLGALIDAGLSLKRLEEALAPLKLPGYR
                     LSAKTVEKAGVRATKVDVAVDDRKVPIRDYTQMVAVFRQGRLPRTAEETALAILERLA
                     RAEADVHGRKLSQLHFHELGGVDTLVDIAGAVLGLQLLGVDEVYASPLNIGGGLTRSA
                     AFPIPAPATAALLSGVPVYSSGIDRELTTPTGAAIIGALAKGFIPFPACRVEAIGHGA
                     GETELPATPNVLRLFVGERLAERAWGEDRVVQIETNVDDVNPQIYEYLMERLQQTGAI
                     EVYFTPIIMKKGRPGITVNVLARAEHSARLARVLFDETPTLGVRVREVDRLVLARTLQ
                     TVSTPWGRVRVKVTRHDGREELRAEYEDCRALAEISGRPLRTVMQEVSTFLRTLAGEA
                     PLEPTVVPPALPEVVSEGGMAWGEPIEGELEASPAEAPTAGGQAAGAAGRRRRSRHRR
                     RRSRGRGNSNKTPPA"
     gene            4568..5569
                     /locus_tag="AC16BC_05515"
     CDS             4568..5569
                     /locus_tag="AC16BC_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008273 - calcium, potassium:sodium
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006874 - intracellular calcium ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR3241112.1"
                     /translation="MTLAILITTMIVAIIVILAGSALFTNGVEWLGKRLGVSDGVVGS
                     VFAGVGTALPETVIPIIAIFLGEGADREAIGIGAILGAPFMLSTLTLPLLGAGLLAFA
                     AMGRRPSVFKLDAAAVSIDLRFFLVGYTLAVGSTMIDSKAVHVVVAIGLIVLYLIYLK
                     TIVGHDGEGHGEALAPLYFARHKAEPPAGLIAFQVGAALAVIIGGAHLFVGMVQSVAT
                     HVGLSPLLLSLLVTPVATELPEKLNSLIWIAQKKDSLAVGNITGAMVFQGTFPVAVGL
                     VGTPWQLNTYGMVSAWTALIAGAVLYATIATRGTWRPAYLMAGALLYLAFGVYVANW"
     gene            5643..>6889
                     /locus_tag="AC16BC_05520"
     CDS             5643..>6889
                     /locus_tag="AC16BC_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3241113.1"
                     /translation="MRDSVQRRIVLSILIVGVASLLLGLSLVYFVGKSTLKRTIGANF
                     EELADVTAKKLDELISHHVERAVFLAGLGEVLDAVRQENAEPVSPSADARLQSEWPQL
                     GASDREVARVVDNPAANAFRSMMRMSGDQTVEHQPHEIVIAADAHGRVVAATRKPSAY
                     LFNDTIWWREAYASGQGRLFLSDVFKDEGLGRYVFIIATPIRRDGRVEGVLAMVHDAQ
                     AFYQWVTSIQVGRTDHTMLVASDGTLLFCPVFPIKSHTLHPTLVASIVSGGRGWGTTT
                     HDVHYPGAEAINGFAPVRASTTPGTNFGGKVWYIFTSQDPKETYGPIYTLLGWIIFSG
                     AAAIGLLILLGLAAAKRIVRPIRIVQSGAQVIGNGNLNHRIHVETDDEIGALARGINE
                     MAAKLSTSYNELERMVEERTRTLA"
BASE COUNT         1100 a         2125 c         2446 g         1218 t
ORIGIN      
        1 ggggatcgaa gtggatacgc tcgcgctcat gaccgacgtg gtgcgtgaat tgctccccgc
       61 cgggagccgc cacccggtcg cggtgctgtc gggcccgtcc ttcgccgccg aggtggcgaa
      121 gggacacccg accgcggtca cgctggcatg ccgggacccg cacgcagcgc ggcgtctgca
      181 agagctcttc accaccgcga cgtttaagtt gttcaccacg ccggacgtca ttggggtcca
      241 ggtgggcggc gcgctcaaga acgtcatcgc gctcgcggcc ggggggtcgg acggcttggg
      301 gttcggggcc aacacacgcg ccgcgctgat cacgcgcggc cttgccgaga tctcgcggct
      361 gggcgtcgcg atgggggggc aaccctggac cttttcgggg ctttccggac tgggcgactt
      421 ggtgctgacc tgtacggatc agcaaagtcg caacttcacg gtgggctatc ggatcggtcg
      481 gggtgaaacc ctggagacca tcacgcgggg cgcgagcacc gtggccgagg gggtccgcac
      541 cgcgcgcgcg gcctatgcgt tgtcgcgcaa ataccgggtg ctgatgccga tcgtggagca
      601 gatctacgcc gtgctctatg aggggaaaga tccgcgcaag gccgtcaccg acctcatgga
      661 acccgcggtc ggggacgagt tgttccggcc gcaggagcgc ggaggcgggt ggggcgaccc
      721 ggccgacgcg cgatgaacga tatcgagcgg gtgttgctcg aactggccgg aggggtcagc
      781 ctcctgcgct acggcttgca cctcgcgggc gaggggcttc aggctctggc gggcgcccgg
      841 cttcgttacg cgctcgcgtc cgtcaccaag aaccgcgtgc tgggcctggg tgccggcgcg
      901 ctgattaccg cgatccttca gagcagcagc gcgaccaccg tgatgctggt gggttttacc
      961 ggctccggtc tgctcacgct ccgccaagcc atggccgtga tccttggcgc ggacgtgggc
     1021 accaccgcga ccgtgcagct gctggcgttc ccggtgagcg agtatgcctt gggcgtgatc
     1081 gcgatcgggt ttttgttgtt ttttttcggc gaacgccagc gaaccaagac cctcggcacc
     1141 gcgttgttgg gattcgggtt gattttcctc ggcttgcggc tgatcgcctc gggcgccgaa
     1201 cccttggtgg actccccgct ggtcggacaa gccttcatgg cgttgggcga tcaccccgtg
     1261 ctcgggatcg cggtgtcggc cacggtcacc gccatgctgc acagcagcgc cgccacgatc
     1321 ggcatcgcgc tcgcgctctc cgcgcacggc ctgttgacat tgcacggcgc gctgccgatc
     1381 atcctgggcg ccaacgtggg gacgtgcgca cccgcgctca tctcgagcct gggaggcgtg
     1441 cccgaggcca agcgggtggc gctcgcccac gcgctgttta aggtggctgg cgtggtgctg
     1501 gtgtacccgg tgttgccgtt gttcgagacc tgggtcgaag cgtcggcggt gagcctggcc
     1561 caccggatcg cgaacgccca caccctgttc aatctcggcc tggccgttgc gtttctcccg
     1621 tttgaagggc cgtttgccgg ggtcgtggct cgcctggtga aggagcggcc gcgcgccgat
     1681 gagtgggcgc ggccgaaata cctcgacccc cacgtgttgg acgtcccgtc gctggcgttg
     1741 agtcaggcca cccgggagac gctgcgtatg gcggacctgg tgcatgaaat ggtgcgtgat
     1801 accatcaagg cgtttgcgga cggcgatcag gagttggttg agtcgatcga gcgcaaggaa
     1861 gactgggtgg acgccctcaa ccgggagatc aaactctata tcactaaact gtcggagaag
     1921 tcccttacca aggagcaact cgaccgcgag atggtgttgc tcgcggtgat caacgatctc
     1981 gagaatatcg gggacatcgt cgacaagaat ctgatggaat tggccaagaa aaaactgtat
     2041 aaggatctga ggttttcgga gaacggcgtg cgcgaaatag tggaattaca cacgctggtc
     2101 gagaggaatt tcgagcgcgt gatcgcggcg tttgcgagtc aggacgcgga ggtggccaag
     2161 caggtgatcg aggaaaaggc gaggatcagt tataaggaac ggcaactcaa gcaggcgcac
     2221 atccaccgct tgcacgcggg gcttccggag tcgatcgaga cgagcgccat tcatttggat
     2281 gtgttgacca atctgaagcg catcaactcc cacgtcacca acatcgcgta cccgctcgtt
     2341 gatcagccct cggaggggtg atggaccgcg ggcggctgcg cgcgctcctg gaagcggttc
     2401 gcgacggcgc ttccgacgtg gatggcgcgc tcgcggcgct tcgcgatttt ccgtccgaag
     2461 ccctcgggtt tgccgcgctg gatcaccacc gcgacttacg ccaaggcgtt ccggaggccg
     2521 tgttgtgcga gggcaagacc gcggccgagg cggcggccat cgtcaagcgg ttggccgtgt
     2581 ccggggcgcc cgtgatcgcc acccgcgcgg aacccgcggt cgcgcgcgca atccgcaaag
     2641 tcgcgccaga cgcgacgtac cacccgcggg cgcgggtgtg catcctacat cccaggtcgg
     2701 cgtttgatcc gcacggcgcg gtggtggtga cggctggcac cgcggatctg ccggtcgcgg
     2761 aagaggcggc gctcacggcc ggcgcgctcg gcaccggggt ttccctggtg tgcgacgtgg
     2821 gcgtggcggg cgtgcatcga ttggtcgatc agttggatcg cctgcgagca gcgcgggtgg
     2881 tgatcgtggt ggcggggatg gacggcgcgt tgcccagtct ggtgggcggc ctgatggcgc
     2941 agcccgtgat cgcggtgccc accagccgcg ggtacggggc gcatttcgga ggcatcgccc
     3001 ccctgctcac gatgctcaat tcgtgcgcgg ccgggttggc ggtggtcaac atcgataacg
     3061 gatttggcgc gggcgtgctg gcccaccgca tcgagcggtt ggggcggcaa ccggcggggg
     3121 acaatggttc acccaatgga cggcgcccgc gccggtcacg acatggataa cacatgaaga
     3181 tcgcatattt tgactgtttt tcgggcatca gcggcgacat ggtcttgggc gcgctgatcg
     3241 acgcgggatt gagcctcaaa cggttggaag aggcgctcgc gccgctcaaa ctcccggggt
     3301 accggctgtc agccaagacg gtggagaaag ccggggtgcg cgccaccaag gtggacgtgg
     3361 cggtggacga ccgcaaagtt ccgatccggg attacaccca gatggtggcg gtgttccgtc
     3421 aaggtcggct gcccaggacc gcggaggaga ccgcgctggc gatcctggag cgcctggcgc
     3481 gcgccgaggc cgacgtgcac ggccggaaac tgtcgcaact gcactttcat gaactgggcg
     3541 gcgtggacac gttagtggac atcgctggcg cggtgctcgg gttgcaattg ttgggcgtgg
     3601 acgaagtcta cgcgtcgccg ttgaacatcg gcggcggcct gacgcgttcg gcggcgttcc
     3661 cgatcccggc cccggccacc gcggcgttgc tctccggcgt gccggtctat tcctccggga
     3721 tcgaccgcga actgaccacg ccgaccggcg cggcgatcat cggcgccctg gccaaggggt
     3781 tcattccgtt tcccgcgtgc cgggtcgagg cgatcggcca cggcgcgggc gaaaccgagc
     3841 tgcccgcgac gcccaacgtg ttgcgcctgt tcgtgggaga gcggctggcc gagcgggcgt
     3901 ggggcgagga ccgcgtcgtc cagattgaaa ccaacgtcga cgacgtcaat ccccagattt
     3961 acgaatacct gatggagcgc ctccagcaga cgggcgcgat cgaggtgtac ttcaccccga
     4021 tcatcatgaa aaaggggcgc cccgggatca ccgtcaacgt gctggcgcgc gccgaacaca
     4081 gcgcccgact cgcccgcgtg ttgttcgacg aaaccccgac gctgggcgtc cgcgtgcgtg
     4141 aagtggaccg actggtgttg gcccgcacgc tgcagaccgt ctccacgccg tgggggcgcg
     4201 tgcgggtgaa ggtcacgcgc cacgacggcc gcgaagaatt acgcgcggaa tacgaggact
     4261 gtcgcgcgct ggcggaaatc agcggccggc cgctgcggac ggtgatgcag gaggtgtcga
     4321 cctttctgcg caccctggcg ggcgaagcgc cgctggagcc gaccgtcgtg ccaccggcgt
     4381 tgcccgaggt ggtcagcgag ggcgggatgg cgtggggcga accgatcgag ggcgaactgg
     4441 aggcctctcc ggccgaggcc ccgactgcgg gcggccaggc ggcgggcgcg gccggccgcc
     4501 ggcgccggtc gcgtcaccgc cgacgccgat cccgaggacg ggggaatagt aacaagactc
     4561 cacccgcatg accctggcga ttctcatcac cacgatgatc gtggcgatca tcgtgatcct
     4621 ggcgggaagc gcgctgttta ccaacggggt ggagtggctg ggaaagcggt tgggggtgtc
     4681 ggatggcgtg gtgggcagcg tgttcgccgg cgtcggcacc gcgttgccgg agaccgtgat
     4741 cccgatcatc gccatttttc tcggtgaagg ggcggatcgg gaagcgatcg ggatcggcgc
     4801 gatcctcggc gcgccgttca tgttgagtac gctgacgctg ccgctgttgg gcgccgggct
     4861 cctcgccttc gccgcgatgg gccgacgccc gtcggtcttc aagttggacg ccgccgcggt
     4921 gtccatcgac ctgcgattct tcctggtcgg ctacacgctc gcggtgggat cgaccatgat
     4981 cgattccaag gccgtgcacg tggtggtggc gatcggcttg atcgtcctgt atctgatcta
     5041 cctcaaaacc atcgtggggc acgacggaga aggacatggc gaagcgttgg cgcccctgta
     5101 cttcgcgcgg cacaaggcgg aaccgccggc gggcctgatc gccttccagg tgggcgccgc
     5161 gctggcggtc attatcgggg gcgcgcacct gttcgtgggc atggtgcaat ccgtggccac
     5221 gcacgtgggg ctctctccgt tgctgctctc gctcttggtc accccggtcg ccaccgagtt
     5281 gccggaaaag ctcaacagtc tgatctggat cgctcagaag aaggattccc tggcggttgg
     5341 aaacatcacc ggcgcgatgg tgttccaggg cacgtttccg gtggcggtgg ggctggtcgg
     5401 cacgccgtgg caattgaata cctacggcat ggtgagcgcc tggactgcgt tgatcgccgg
     5461 ggccgtgctc tacgcgacca tcgcgacccg gggcacctgg cggccggcct acctgatggc
     5521 gggcgcgttg ttgtatctgg cgttcggcgt ctacgtggcc aactggtaac cccggtcttg
     5581 cgggtcggct cgcgttcgtg actttcggaa tggtacactc tgacgttcgt cgggagtgag
     5641 cggtgcgcga cagcgttcaa agacggatcg tcttgtcgat cttgatcgtg ggggtggcgt
     5701 ccctgctgct cgggctgtcc ctggtctatt ttgtcgggaa atccacgctc aaacggacga
     5761 tcggcgccaa cttcgaggaa ctcgccgacg tcacggccaa gaagctcgat gaactcatca
     5821 gccaccacgt cgagcgagcg gtctttctgg ccggcctcgg cgaagtgctg gacgcggtgc
     5881 ggcaagaaaa cgccgagccc gtgtccccct ccgcggacgc gcgcctgcag tccgaatggc
     5941 cgcagttggg cgcctccgac cgtgaggtcg cgcgggtggt ggacaatccc gcggcgaacg
     6001 cgtttcggtc catgatgcga atgtccggag accagaccgt cgaacaccag ccccacgaga
     6061 tcgtgatcgc ggccgacgcc cacggacgcg tggtggctgc cacccgcaaa ccctcggcgt
     6121 acctgttcaa cgacacgata tggtggcggg aagcctacgc cagcggtcag ggccgcctgt
     6181 tcctgagcga tgtgttcaag gacgaggggc tgggccggta cgtgttcatt attgccacgc
     6241 ccatccggcg cgacggtcgc gtggagggcg tgctcgccat ggtccacgac gcgcaggcgt
     6301 tctatcaatg ggtcacgtcg atccaggtcg gacgaaccga tcacacgatg ctggtggcct
     6361 cggacggaac cctcctgttc tgcccggtgt tccccatcaa gagccacacc ctgcacccga
     6421 cgctggtcgc ttcgatcgtg agcggcggac gcgggtgggg gaccacgacg cacgacgtgc
     6481 attatcccgg cgcggaggcg atcaacggtt ttgcgccggt ccgggcgtcc accacgcccg
     6541 gcaccaattt cggcggcaag gtctggtaca tcttcacgag ccaggacccg aaagagacgt
     6601 atggcccgat ctacacgctt ttggggtgga tcattttttc cggggccgcg gccattggtc
     6661 tgttgattct cctcgggctt gcggcggcca aacgcatcgt ccggccgatc cggatcgtgc
     6721 agagcggggc gcaggtgatc ggcaacggca acctgaatca ccggatccat gtcgagacgg
     6781 atgacgagat cggcgccttg gcgcgcggga tcaacgaaat ggcggccaaa ctctccacct
     6841 cctacaacga gttggagcgg atggtggagg agcggaccag gacgctcgc
//
LOCUS       DBNGTT010000246         3438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1970588, whole genome shotgun sequence.
ACCESSION   DBNGTT010000246 DBNGTT010000000
VERSION     DBNGTT010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1970588"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="AC16BC_05525"
     CDS             complement(<1..687)
                     /locus_tag="AC16BC_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241114.1"
                     /translation="MHPRLRRRLRPIAAIVLVFFTWFSIEPWNYALAAQDSTAPRPGR
                     ASASQSKAPTAAEVFETNLRSIKEQVRAARDGDAYQAQLTGLLKSLDQAVAQSSRELE
                     ATRQAIDRLARAIEHGTPSQDAIKAVQSQQQAVKAAVGQVRLNASGIEGLLRGMALPA
                     GLAKDQKQAKETVSSLLKAFDTAIAAMAKRAEQQRGFDAQEHQTFLAASMELDQGLAA
                     LQARVQALAKE"
     gene            1038..1691
                     /locus_tag="AC16BC_05530"
     CDS             1038..1691
                     /locus_tag="AC16BC_05530"
                     /inference="COORDINATES: protein motif:HMM:NF022747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR3241115.1"
                     /translation="MGESRMHRRLAGIVTASALLMVGFAGHVSAAEAPKAKPAVTPQK
                     PDGKPGRAVETLGNEHVQSPGSPHVPYNTSPPTSGPHLQWVAKWGVSKSPILRELQVH
                     NLEDGGVVIQYKCDAGCPDLVAKLEGLAAKYAEKAEADRKTMPPPKDPRFPVRSKYDH
                     LIVAPYPDMANQIALTAWGRIDTFDGYDEARIVKFIEAFIGIDHHPAQEMAPAAKTP"
     gene            1688..3292
                     /gene="lnt"
                     /locus_tag="AC16BC_05535"
     CDS             1688..3292
                     /gene="lnt"
                     /locus_tag="AC16BC_05535"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR3241116.1"
                     /translation="MTTALSRRQLAGAAFSGLLLIVSFPRPQFALAAWLALVPLLLTV
                     SNATSRRDAFLLGWITGVTFFTGSLRWLAYTMVHYGHLPEWLSYIVLLLLTAYLGVYV
                     GLFALAARVLNDQPTWLRLTALPAAWAALELARTHLLTGYPWVLLGYSQAGTLPVIQI
                     AEFTGVYGVSYLIVLVNAALAIALTAPRPAGEMPMPIPVASFVPMPAPSLPRRGLAAL
                     FAAALVLIGVLGYGHWRLDQLLRTSAETPPLNVAVIQGNVDQDHKWDPAFLRETIDRY
                     HALTNEQTDRPLDLIVWPEAAMPFIFERDASLRRELLGFVAEHRTPLLFGSPAIDGAD
                     SSTPKMYNSAYLVTATGEVAGRYDKMHMVPFGEYVPLAPLLFFVNKMADGIGEFVPGS
                     DATVFSLERFRLGVMICFEVVFPELTRRTVQNGATVMAAITNDAWFGDSAAPYQHMDV
                     VVLRAVEHRVPFARAANTGISGFIDATGHVETATDLFVPGSRAATLAPRSGETWYTRH
                     GDVFVWACVILAAFALARATRRSTRP"
BASE COUNT          570 a         1152 c         1061 g          655 t
ORIGIN      
        1 ttctttagcc aacgcctgca cgcgcgcttg cagcgcggcc agtccctgat cgagctccat
       61 actggctgcg agaaacgtct ggtgttcttg tgcatcgaac ccgcgctgct gttccgcccg
      121 ttttgccatc gcggcaatgg cggtgtcgaa cgccttcagg agactgctca ctgtctcttt
      181 ggcctgtttc tgatccttgg ccaggcccgc cggcaaagcc atgccccgca gcagcccctc
      241 gatccccgag gcgttcaagc gtacttggcc gaccgcagcc ttcaccgcct gctgttggga
      301 ttgaacggct ttgatcgcgt cttggctggg cgttccgtgc tcgatggccc gcgccagtcg
      361 atctatagct tgtcgtgtcg cttccaactc gcggctgctc tgggccaccg cttgatccag
      421 gcttttgagc aggccggtca actgcgcttg gtaggcgtcc ccgtcccgcg ctgcacggac
      481 ctgttccttg attgacctga ggttggtctc aaagacttcc gcagcggtcg gtgctttgga
      541 ttgggatgca gacgcgcggc ccggccgtgg ggcagttgag tcctgcgccg ccagcgcgta
      601 gttccacggc tcgattgaaa accaggtgaa gaagacgagg acaattgccg cgatgggccg
      661 taggcggcgc cttagtcgag ggtgcatggt cccctctgag acgcgcgggt cggccactgt
      721 gggccgacaa cggaggattc taagtacgcg aaaggaaggc gacagtctgt cgcccgggac
      781 cgcaacgtcc aatacaccga atcattcctc ctggaccatc ggcagcgagt aaccgctcgg
      841 ccataccaaa acggagttgg cgtaaggctc gtaacgttac gagggcgact tgtagcacag
      901 cctgaacagg ggtgtcaatc agttttcttt tcagttttct tttcgggaga gatcgcggtt
      961 tgatctgaga accgagccca tagacgcatt ctttactttc aattcccgcg ttgttaccat
     1021 ctcacgacgt gttgatcatg ggagaatcgc gtatgcatcg acggctggcg ggtattgtca
     1081 cggcatcggc actattgatg gtgggcttcg ctgggcacgt atcggccgct gaggctccca
     1141 aggccaaacc cgcggtcacg cctcagaagc cggacggcaa acccggaagg gcggtggaaa
     1201 cgctcggcaa cgagcacgtg cagtctcccg gttccccgca cgtgccctac aacacgtccc
     1261 cgcccacctc gggcccgcat ttgcagtggg tggccaagtg gggcgtgagc aagagcccga
     1321 tcctgcgcga actgcaagtg cacaatctgg aagacggcgg cgtggtcatc caatataagt
     1381 gcgacgcggg ctgccccgac ctggtggcca aactggaggg cctggccgcg aaatacgcgg
     1441 agaaggccga ggcggaccga aagaccatgc cgccgccgaa agacccgcgg tttccggtac
     1501 gcagcaagta cgatcatctg atcgtagcgc cgtatcccga catggcgaat caaatcgccc
     1561 tgaccgcgtg gggacggatc gatacgttcg acgggtatga cgaagcccgg atcgtgaagt
     1621 tcatcgaagc ctttatcggc atcgatcacc accccgccca ggagatggct cccgccgcga
     1681 agaccccgtg acgaccgccc tctcccggcg gcagctcgca ggcgcggcgt tctccggcct
     1741 gctcctgatc gtcagttttc cccgtcccca attcgcgctc gccgcgtggc tggcgctggt
     1801 tcccctgctg ctcacggttt cgaacgccac atcccgacgt gacgcgttcc tgctcggatg
     1861 gatcaccggc gtcacgtttt tcaccggctc gttgcggtgg ctcgcctaca ccatggtcca
     1921 ctacgggcac ctgcccgagt ggctgagcta tatcgtcttg ctgctcctga ccgcgtatct
     1981 gggggtgtac gtcggcctgt tcgcgctggc ggcccgcgtc ctgaacgatc agcccacctg
     2041 gctccgcctg accgcgctgc cggccgcgtg ggccgcgttg gaactggcgc ggacgcatct
     2101 cctgacaggc tatccgtggg tgctgctcgg ctactcgcag gccggcaccc tgccggtgat
     2161 ccagatcgcg gagttcaccg gcgtgtacgg ggtctcgtac ctgatcgtgt tggtcaacgc
     2221 cgcgctcgcc atcgccctga ccgccccgcg cccggccggt gaaatgccca tgccgatccc
     2281 ggtcgcctcc ttcgtgccca tgcccgcgcc gtcgctgccg cggcgcggct tggccgcgtt
     2341 gttcgcggcg gccctcgtcc tcatcggagt gctgggctac ggacactggc ggctcgatca
     2401 actgctgcga acgagcgcgg aaaccccgcc gctcaacgtc gcggtgatcc agggcaacgt
     2461 ggaccaggat cacaagtggg accccgcgtt tctgcgggag acgatcgacc ggtaccacgc
     2521 gctcacgaac gaacaaacgg accgccccct cgacctgatc gtatggcccg aagccgcgat
     2581 gccgttcatc ttcgaacgcg acgcctcgct ccgccgggag ctgctcggct ttgtggccga
     2641 acaccgaacg ccgctgctct tcggcagccc cgcgatcgac ggcgcggact cgtccacgcc
     2701 gaagatgtac aacagcgcct acctcgtcac cgcaaccggc gaggtggccg gccggtacga
     2761 caaaatgcac atggtgccgt tcggcgagta cgtcccgctc gccccactgc tctttttcgt
     2821 gaacaagatg gccgacggaa tcggggagtt cgttcccggc tccgacgcca ccgtgttctc
     2881 gctggagcgg tttcgcctcg gcgtcatgat ttgcttcgaa gtggtgttcc ccgaactgac
     2941 gcgacgcacg gtgcaaaacg gcgccacggt gatggcggcc atcaccaatg acgcgtggtt
     3001 cggggattcg gccgcgccgt accaacacat ggacgtcgtg gttttgcgcg cggtggagca
     3061 ccgcgtgccg ttcgcgcgcg cggccaacac cgggatctcc gggttcatcg acgccacggg
     3121 ccacgtggaa accgccaccg acttgttcgt gcccggctcg cgcgcggcca cgctcgcgcc
     3181 ccgctccggc gagacgtggt acacgcgcca cggcgacgtg ttcgtctggg cgtgcgtgat
     3241 cttggccgca ttcgccctgg cgcgggcgac tcgacgatcc actcgaccat gacgagcgac
     3301 cgcgcgacgt gatagaatcc ccgcgcatat tcacgctcaa ggaaggaata ccgtgattct
     3361 cgaagattac cacagccgct tcgaatcgct ccgccagcgc accatcgccc tgcgggggta
     3421 tctttgaccc ggctcgcc
//
LOCUS       DBNGTT010000247         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1076323, whole genome shotgun sequence.
ACCESSION   DBNGTT010000247 DBNGTT010000000
VERSION     DBNGTT010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1076323"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..920
                     /gene="accD"
                     /locus_tag="AC16BC_05540"
     CDS             54..920
                     /gene="accD"
                     /locus_tag="AC16BC_05540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR3241117.1"
                     /translation="MAWFQKKDKDNEKEPSPGDDVAGEKRGKSSMEGLWVKCNHCREI
                     IYRKELDRNLKVCPKCDYHFPITVEERIGLLLDEGSFKEWDADLSPADPLGFRDTQRY
                     RDRLKTAQEQTGRRDAMVSGSGTVEGRTIALCVFHFGFMGGSMGSVVGERIVRAAERA
                     EANRSPLVVVTASGGARMQEGILSLMQMAKTSAAVGRLQRAGLPYVSILTDPTYGGVS
                     ASIATLGDVILAEPKALIGFAGPRVIEQTIKQQLPPGFQRAEFLLEHGMIDAIVERKK
                     MKGTLASLLSCL"
     gene            1131..2429
                     /locus_tag="AC16BC_05545"
     CDS             1131..2429
                     /locus_tag="AC16BC_05545"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3241118.1"
                     /translation="MSYQETVEFLYRLQWHGIRMDLSPARGLLDAVGRPHERYPVLHV
                     GGTNGKGSTAAMLASMLREAGYTVGLYTSPHLVDFTERIRINGAAITPDHVVSLAEEL
                     RARMPAGEDPTFFEFTTAMAFLAFARARVDAAVIEVGMGGRLDATNVVRPLVSVITNV
                     DLDHQRYLGDTVTTIASEKAGIIKPGTPVVTASGSPEVLAVLADRATAQGSSITVVGR
                     DVRVEGAGPEAFSYQGRAGRIDGLTCPLLGRHQLTNAATALAAVEHARDRGLAIPIDA
                     IRRGLARVRWEGRLERIKSRPTILVDGAHNAAGAAALADFLAAERAAGRINRLVAVFG
                     ILADKDLHTIVERVGALADEVVLTEPSHHRAAPIDTLREAASSLSVPIRLAPTVADAL
                     ADVEARLSPRDWCVVTGSLYTVGEVKALYQGRGRLSLLRG"
BASE COUNT          436 a          830 c          946 g          451 t
ORIGIN      
        1 gccgcatccg ccctctgtgt gataccgtga tgcctgtttt ttgaaggaaa accgtggcct
       61 ggtttcagaa aaaagacaag gataacgaga aggaaccgtc gccgggcgac gacgtcgcgg
      121 gggagaagcg cggcaagtcg tccatggaag ggctgtgggt caagtgcaac cactgccgcg
      181 agatcattta ccgcaaggaa ctcgaccgca atctcaaagt ctgtcccaaa tgcgactacc
      241 acttcccgat cacggtggag gagcgcatcg ggctgttgct ggacgagggg agtttcaagg
      301 agtgggacgc cgacctttcg ccggccgatc cgctggggtt tcgcgacacc cagcggtacc
      361 gcgatcggct gaagacggcg caggaacaga ccggacgccg cgacgcgatg gtgagcgggt
      421 ccgggacggt cgagggccgg actattgcct tgtgcgtgtt tcacttcggg ttcatgggcg
      481 ggagcatggg ctcggtggtg ggggagcgga tcgtgcgcgc ggcggagcgg gccgaggcca
      541 accgctcgcc cctggtggtg gtgaccgcgt caggcggcgc ccgcatgcag gaaggcatcc
      601 tgtcgttgat gcagatggcc aagacctccg cggcggtggg gcggctgcag cgggccgggc
      661 tgccgtacgt gtcgatcctg accgatccca cctacggcgg cgtgtccgcc agcatcgcca
      721 ctttgggcga cgtgatcctg gccgagccca aggcgctgat cggcttcgcg ggcccccggg
      781 tgatcgagca gaccatcaaa caacagttgc ctcccggatt tcagcgcgcg gagtttctcc
      841 tcgagcacgg catgatcgac gcgatcgtgg agcgaaagaa gatgaagggt acgctcgcct
      901 cgctcctctc ctgcctctaa tcgccggccg atgagggccc atcttcggcg ttgccggctg
      961 cgcatccgat cctcacgtac gacacagtac gctccggtgc ttcgccaagc gatcggaacg
     1021 agacatcgac agcgccacaa agcggcgcag tgcgcttttc ggtgctcgcc ggcgcctggc
     1081 atctgggccc tcctcagccg gctcacggaa agaatcaacc caacaaaacg atgagttacc
     1141 aggagacggt cgagtttttg tatcggttgc agtggcacgg gattcggatg gatttgagtc
     1201 ccgcgcgcgg gctgctcgat gcggtggggc ggccgcacga gcggtatccg gtgttgcacg
     1261 tgggcggcac gaacggcaag ggatcgaccg cggccatgct ggcgtcgatg ctccgggagg
     1321 ccggatacac ggtgggtctg tacacctcgc cccatctggt ggacttcacc gagcggatca
     1381 gaatcaacgg cgcggcgatc acgcccgacc acgtggtgtc gttggccgag gagttgcgcg
     1441 cccggatgcc ggccggcgag gaccccacgt ttttcgagtt caccacggcc atggcgtttc
     1501 tcgcgttcgc gcgcgcgcgc gtggacgcgg cggtgatcga ggtgggcatg ggcgggcggt
     1561 tggacgccac caacgtggtc cggccattgg tgtcggtgat caccaacgtg gacttggacc
     1621 atcagcggta tctgggcgat acggtgacca cgattgcctc ggagaaggcc ggcatcatca
     1681 aaccgggcac gccggtggtg accgcgtccg ggtccccgga ggtgctcgcg gtgttggccg
     1741 atcgcgcgac cgcccagggc tcgagcatca ccgtggtcgg acgcgacgtc agggtggaag
     1801 gggccgggcc cgaggcgttt tcgtaccagg gacgcgccgg tcgcatcgac ggcctgacct
     1861 gtcccttgtt ggggcggcac caactgacga acgcggccac ggcattggcc gcggtggaac
     1921 acgcccgcga tcgaggcctg gcgattccca tcgacgcgat ccgccgcggg ctggcccgcg
     1981 tgcggtggga gggacggctc gaacgcatca aatcccgtcc gaccatcctg gtggacggcg
     2041 cccacaacgc ggccggcgca gccgcgctgg cggactttct cgccgcggag cgcgcggcgg
     2101 ggcggatcaa tcgattggtg gcggtgttcg gcattctggc cgacaaggat ctgcacacca
     2161 tcgtcgagcg cgtgggcgca ctggccgacg aggtcgtgct gaccgaaccg tcccatcacc
     2221 gcgcggcgcc gatcgacacg ctgcgggaag ccgcgtcctc gctgtcggtt ccgatccggc
     2281 tcgcgccgac cgtcgccgac gcgctggccg acgttgaagc gcgcctgtcg ccacgagact
     2341 ggtgcgtggt gaccggatcg ctctacacgg tgggcgaggt gaaggcgctg tatcaggggc
     2401 gtggccggtt gtcgctcttg cgcggatagg acggagcctg tccatgaacg gtcatctgcg
     2461 gcgttgccgc tgcgcatccg ctgctcacgt acccgcttcg tacgctgcgc tgcggtgctc
     2521 gcggcgcctt gcgacccacc gccaggaatt caaatggcgg aagtccccga agggggcatc
     2581 tgacgcgttc ctgaacaggc tccagtgttg gaagacctgg ctactcgcgt agtgggggtg
     2641 ttccgcgctc cacggccggc gat
//
LOCUS       DBNGTT010000248         4065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1588168, whole genome shotgun sequence.
ACCESSION   DBNGTT010000248 DBNGTT010000000
VERSION     DBNGTT010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1588168"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="pssA"
                     /locus_tag="AC16BC_05550"
     CDS             complement(<1..691)
                     /gene="pssA"
                     /locus_tag="AC16BC_05550"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR3241119.1"
                     /translation="MTTRPRRGVYLLPNLFTTGNLLSGFYSVVAVFNSDYVSAAIAIL
                     VATVFDSLDGRVARMTKTTSKFGVEYDSLADLVSFGVAPGVLIYSWALTNYGRVGWVA
                     AFLFVACGALRLARFNVQVGSVDSRYFVGLPIPAAAGMLASTVLFDDHILRLGAEMKP
                     MLIVVMTYVLAFLMVSNIRYSSFKGLEFRNRRPFQALVAAVLVVMLVTAAPQVMLFAL
                     FGLYAASGVVMG"
     gene            complement(688..1344)
                     /locus_tag="AC16BC_05555"
     CDS             complement(688..1344)
                     /locus_tag="AC16BC_05555"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HIR3241120.1"
                     /translation="MSPSEAWQESPVVIEGLPFVLAPTALAGMAWWAGWGAVAVLCAG
                     AALFTVWFFRNPRRTVPDDPRAIVSPADGKVLSVERLFESRVLKDDVQRVSIFMGLTD
                     VHVNRIPSDGRIVGRYYYPGKFLSAFKEKASLDNEQQALVLETPDGRRVMFVQIAGLV
                     ARRIVTWVREGDQVERGRRYGLIRFGSRMDVYLPLNVQVTVKPGDRVAGASSVLGVWP
                     "
     gene            complement(1347..2363)
                     /gene="ilvC"
                     /locus_tag="AC16BC_05560"
                     /pseudo
     CDS             complement(1347..2363)
                     /gene="ilvC"
                     /locus_tag="AC16BC_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            complement(2407..2934)
                     /gene="ilvN"
                     /locus_tag="AC16BC_05565"
     CDS             complement(2407..2934)
                     /gene="ilvN"
                     /locus_tag="AC16BC_05565"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR3241121.1"
                     /translation="MEHIISLLAENKFGVLARVAGLFSARGFNIESLSVGPTADPTMS
                     MITIVTTGDDRIIEQITKQLNKLIDTIKVVDLTEHSYVERETVLVKIHTRAEDRAEVF
                     RIIEIFRARIVDSTPSTYTVESTGDREKVEAFINQLRPLGIKELVRSGRIAIMRDEVR
                     AVTETRRRPQVVESK"
     gene            complement(2953..>4065)
                     /locus_tag="AC16BC_05570"
     CDS             complement(2953..>4065)
                     /locus_tag="AC16BC_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3241122.1"
                     /translation="DASEELVALAELLNNPVTMTLMGLGAFPGTHPLSLGMLGMHGSH
                     ATNLAVHNCDLLVAVGARFDDRVTGKISEFVPSAKIIHVDVDPASIRKNIHVDIPIVG
                     DAKPVLRDLLMQVTEVVGGKKREGKTPWLQQIAAWESERPLAYKPDPKVIKPQFVIDM
                     IYRRTRGEAIITSDVGQHQMWTAQFYKFSKPRTWLNSGGLGTMGFGFPAALGAQRAFP
                     DKRVVCITGDGSFQMNLQELATAMVYDLPVKVAILNNGYHGMVRQWQQLFYQGHYSAS
                     RLEGGPDYVKLAEAYGALGLRATEPHEVDEVLDEAFASKKLVLMDFQVDPYENCYPMV
                     PAGGAIHQMMYEDPPSGAGRQAPASQEAQKESISLA"
BASE COUNT          798 a         1362 c         1134 g          771 t
ORIGIN      
        1 gccccatgac cactccggaa gccgcgtaca gaccaaacag agcgaacaac atgacttgag
       61 gcgcggcggt gacgagcatc accaccagca ccgcggcgac caacgcctga aacggccgac
      121 gattccgaaa ctccagccct ttgaaactcg aatagcggat gttgctcacc atcaagaagg
      181 ccagcacgta ggtcatgacc acgatcaaca tgggtttcat ttccgccccg agccgcagaa
      241 tgtggtcgtc gaacaacacg gtcgacgcca acatcccggc cgcggcggga atcggcaggc
      301 ccacaaagta ccggctgtcc acgctcccca cctgcacgtt gaaacgcgcc aggcgcaacg
      361 cgccgcacgc gacaaacagg aacgcggcca cccatccgac ccgcccgtaa ttggtcagcg
      421 cccacgagta gatcaacacg ccgggagcca cgccgaacga caccagatcg gcgagcgaat
      481 catactccac gccgaactta ctggtggtct tggtcatgcg cgcgacgcgg ccgtcgaggc
      541 tgtcgaacac cgtggccacc agaatcgcga tggccgccga gacatagtcg gaattgaaga
      601 ccgccacgac cgaatagaag ccgctgagca ggttgccggt cgtaaagagg ttcggcaaca
      661 aatacacgcc gcgccgaggc cgcgtcgtca tggccatacc cccagcacgc tgctcgcccc
      721 ggcgacccga tctccgggct tgacggtgac ctgaacgttc agggggaggt agacgtccat
      781 ccttgacccg aatcgaatca gaccgtagcg acggccccgc tccacctgat caccctcccg
      841 cacccatgtg acgattcgtc tcgccaccaa ccccgcgatt tgcacaaaca tcacgcgccg
      901 tccatccggc gtctccagga ccaacgcttg ctgctcgttg tccaacgacg ccttctcttt
      961 gaacgccgac agaaacttgc caggataata ataccgccct acgatccgtc cgtccgaggg
     1021 aattcgattc acgtgcacgt ccgtcagccc catgaagatg ctcacccgct gcacgtcgtc
     1081 cttgagcacg cgggactcga acagccgctc cacggacagc actttgccgt ccgcggggga
     1141 gacgatggcg cggggatcgt ccggcacggt gcgtcgggga ttccggaaaa accacaccgt
     1201 aaaaagcgcc gcccccgcgc acagaaccgc caccgcgccc cagcccgccc accatgccat
     1261 accggccagg gctgttgggg cgagcacaaa cgggagtccc tcgatgacga ccggagactc
     1321 ttgccacgcc tcggaagggc tcaaatttag tttttgtcct tatcgaccag ccggcgttgc
     1381 ttgagccagg gcatcatcgc ccgaaggcgc ccgccgactt cttcgatggg atgggattcg
     1441 ccttgtcgca gcagggcggt aaactcggga cgcccggcgc ggttctccaa aatccattct
     1501 ttggcgaatc gcccgctctg aatctcgccc agtatccgtt tcatttcggc gcgggtctcg
     1561 tcggtaatga tgcgtggacc gcgggtcacg tccccgtact tcgccgtgtt gctgatcgaa
     1621 taccgcatgt tcgaaatacc gccctcgtag atcaagtcga cgatcaattt gacttcgtgc
     1681 agacattcaa aatacgccat ctcgggcgag tagccggcat cgatcaacgt ttcatacccc
     1741 gcctggatca atgcggtcaa acctccgcag agcaccacct gctcgccgaa gaggtcggtt
     1801 tcgcattcct cgcggaacgt cgtctcgatc acacccgcct tggtcccgcc aatggctttc
     1861 gcgtaggcca aaccgacccg ccgcgcgtta ccggacggat cttggtgcac ggccaggagc
     1921 gacggcacgc cgctgccctt ggcatactcg gaccgcacga ggtgaccagg ccccttgggc
     1981 gcgaccatga acacgttgat gtccgggcgc ggcacgatct gcccaaaatg gatgttgaat
     2041 ccgtgcgcga acgcgagata cccgcccgct ttcatgttcg gcgcgatctc accgtggtag
     2101 atttcggcct ggagttcgtc gggcgccagg atcatcaaca cgtcgcagcg ttttgcggcg
     2161 tcggccaccg gcatgacctt caatcccgcg cgctccgcct tgtcccatga cgcgccggcc
     2221 cggaccgcga ccatcacgtc gatgctgctg tcctggagat tattggcgtg agcgtggccc
     2281 tggctcccat accccacgat cccgacggtc ttgcccttga gatcggccag atccgcgtcc
     2341 tgctcgtaat agatcttcat tactgtctcc cgatgaccac gatcggcctg cgctcggcga
     2401 gccccgttat ttcgactcga ccacctgcgg ccgacgccgc gtctctgtca cagcccgaac
     2461 ttcatcccgc atgatcgcaa tccgccccga cctgaccagt tccttgatgc ccagcggtcg
     2521 gagctgattg atgaacgctt cgactttttc acgatccccg gtgctctcca cggtataggt
     2581 cgacggggtg gaatcgacga tccgcgcacg gaagatctcg atgatgcgga agacctcggc
     2641 gcgatcctcg gcgcgggtgt ggattttgac cagcacggtt tcccgctcca cgtaactgtg
     2701 ctcggtgaga tccacgactt tgatcgtgtc gatcagttta ttcaactgct tggtaatctg
     2761 ctcgatgatc cggtcgtcgc ccgtcgtgac gatcgtgatc atcgacatag tcgggtccgc
     2821 ggtcggaccg accgacagac tttcgatatt gaatccgcgc gctgaaaaga gaccagccac
     2881 tcgtgcgagc acgccgaact tattctcggc caacaacgaa atgatgtgtt ccattgtgac
     2941 ggtccgtccc ggttacgcga gcgaaataga ctctttttga gcctcttggg aggcgggcgc
     3001 ctggcgtccg gctccgctgg gcggatcttc gtacatcatt tgatggatcg cgccgcccgc
     3061 cggcaccatc ggatagcagt tctcgtaggg atccacctga aaatccatca agacaagttt
     3121 cttggacgcg aacgcttcgt cgagcacctc atccacctcg tgcggttccg tggcgcgcag
     3181 cccgagcgcc ccgtacgcct ccgcgagctt cacgtagtcc ggcccgccct ccagccggct
     3241 tgcggagtag tggccctgat agaaaagctg ttgccactgc cgcaccatcc cgtgatagcc
     3301 gttgttcaga atggcgacct tgaccggaag gtcgtacacc atggccgtgg ccagttcctg
     3361 aagattcatc tggaaactgc cgtcgcccgt gatgcagacg acccgcttgt cgggaaaggc
     3421 ccgctgagca cccaacgcgg ctgggaatcc gaatcccatg gtccccagac cgccggagtt
     3481 caaccatgtc ctcggtttgg agaacttgta gaactgcgcc gtccacatct ggtgctgtcc
     3541 gacgtcggac gtgatgatcg cctcgccgcg cgtacggcgg tagatcatgt cgatcacgaa
     3601 ctgcggcttg atgaccttgg gatccggctt gtaggccagc ggtcgctcgc tttcccacgc
     3661 ggcgatctgc tgcaaccagg gggttttgcc ttcgcgcttc ttgcccccca ccacctcggt
     3721 cacctgcatc aacaggtctc gcaatacggg cttggcatcc ccgacgatcg gaatgtccac
     3781 gtggatattc ttgcggatcg acgcggggtc cacgtcaacg tggatgattt tggcactggg
     3841 cacgaactcg ctgattttcc cggtcacccg atcgtcgaac cgcgcgccga ccgccacaag
     3901 caaatcgcag ttgtgcaccg ccaggttcgt ggcgtgtgaa ccgtgcatgc ccaacatccc
     3961 gagcgacagc gggtgagtgc cgggaaatgc gcccaatccc atgagggtca tggtcaccgg
     4021 attattcagg agttccgcga gggcgaccaa ttcctcactc gcatc
//
LOCUS       DBNGTT010000249         6207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2354956, whole genome shotgun sequence.
ACCESSION   DBNGTT010000249 DBNGTT010000000
VERSION     DBNGTT010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2354956"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..3754)
                     /gene="smc"
                     /locus_tag="AC16BC_05575"
     CDS             complement(116..3754)
                     /gene="smc"
                     /locus_tag="AC16BC_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR3241123.1"
                     /translation="MRLTRLEILGFKSFGERTVLSFQPGITAIVGPNGCGKSNIADAI
                     LWCLGEQSPKTLRGDKMEDVLFNGNTRRTATGLAEVSLTLGGVRPGEIEGTAGSYSEV
                     TLTRRLYRSGDSEYLINRVPCRLRDIRDLLIESGAGIKGHTVLEQGKVAEIVTASPIE
                     RRALVEETAGITKYKLRKAEALRKLESTEQNLLRVRDIIGEVKRQLASLDRQVRKAER
                     YRQAADQLRALELRAAAIRYREWFDQAATLAADLERERAEAAGALAQLSALDAATESL
                     KVDGAELEQRLLEARAELASRETAARDGHHRIDVLAAQVEEWHTQQATLATEIEELSR
                     ALAARGEEQTRLNEQAASLDAAIEERTALVADRERAIRHLEHQLAQAAAEIEQTRHRV
                     FEAASSVATATNALASLSVRREELERRRSRYREDIGTVQTEMAHKRGVHHELETSHQV
                     QATSLATRDAERAALREVSATRQAALQEGEAALSGRRDELVRLRATLDSLHALQRDLV
                     GYDEGVRRVLLGDTSTAPVAGLLGVVADVLDIPAEYERAVEAVLTERLQGVVAEDHEQ
                     IVSALSLLDRLDAGRAVFVPKAPRAAVPSVDSDAETLRLDGVRGFASRLVGCRDGFES
                     LRDTLLARVVIVEDRAVARRLWEADQSGMTWVSVSGDVFQPSGVVSGGKASNASLTLL
                     ERKRQIKTLEQEIANTETALRPLEASTASLRDALSADRSVLEALDAEVRDGELRLLHD
                     QKAAEALAQELLQLDRRLALLATEESAVVSELAAMDQEVANHHAALAAGEASKVRDEA
                     ALLGQYEAAAQLKSALATEQTAATELRLALAADREKREHAAATLRAIAERLDEAGRQM
                     DSKGASRTQFDARIAAATLERVRIVDEIAVLDRHVEVLRQRVAELDAARADVSHRVKE
                     MEDQSKAARRHADAVQERVHAHTVSLTEIRLRIGQQLSDIRDRYQVELEAFDADSLPP
                     PTCEADQFDAAITDLRATLVQIGGVNLAAIDEHRELEERGGFLATQEGDLVQSVDNLR
                     AAIAKINATTKTLFLEAFESLNTRFGEVFASFFEGGQAALHLVDETNPLESGIEIVAQ
                     PPGKKLRHISLLSGGEKALTAISLLFASFLIHPSPFCVMDEIDAPLDEENVRRFTRAL
                     RPMTAHSQFLVVTHNRRTMEQADVLYGVTMEEAGVSKIVSVKLHKDRDHDESPAVEVD
                     LATAELSL"
     gene            4000..4239
                     /locus_tag="AC16BC_05580"
     CDS             4000..4239
                     /locus_tag="AC16BC_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241124.1"
                     /translation="MDAATGAVIWHQPESPTPQRSLNDLVKDLEVKRREVADRFERER
                     RSLGDRSRVLDEKVKESLKRVDPNTPPPIRPIDLD"
     gene            4325..5101
                     /locus_tag="AC16BC_05585"
     CDS             4325..5101
                     /locus_tag="AC16BC_05585"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR3241125.1"
                     /translation="MLPQWEIVAAIGVFGALIGSFLNVCIHRLPLGESLVSPRSRCPG
                     CRKPVRAYDNIPIVSFVLLGGRCRSCRQRIAIRYPAVEALNAAGYIFIWYRFGATPDA
                     VVFGAFYSALLVITFIDWDHQIIPNRITYPGIAIGLLSAATVLAGSFVDALLGAILGG
                     GLLYGVAVGSEWVLKKEGMGFGDVKLLAMIGAFLGWRAVLVTIFVGALAGSVIGLTLM
                     ALRIKKRGDPIPFGPFLALGACAALFAGPELIAWYLRLGT"
     gene            5173..>6207
                     /locus_tag="AC16BC_05590"
     CDS             5173..>6207
                     /locus_tag="AC16BC_05590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3241126.1"
                     /translation="MTALGVRRHVFPHPVAERAVSAVDLVIVVSLIALAFAGFMFRRR
                     SRFSVQPAHQEDDRAGFVMHTFQEVLRQLKEKEAELEHLRARAVARAEDVESYHENIL
                     RSIASGVITCDPAGRITTFNAAAERILGLEAAHVVGRSCDEIFGNDSPIPGMVHGSLE
                     ALAPISRREWHFCRRDAHRVCVGLSSALLRDRNDQLIGAALVFTDLTEIKRLEERIES
                     ERRLAMLGEMSAAIAHEFRNYMGTIVGWAKLLTKQVEAGTQAGSMIAAIRRELGMMQR
                     LIDDLLSFGRRLEPQTERVDLRELVAQGMSLPPDRPDVRVETDWAHGAATTAHWDPTL
                     MRQVLKNLAQN"
BASE COUNT         1035 a         1961 c         2029 g         1182 t
ORIGIN      
        1 gatctacgcc agacgggagc tgatggcggg gtcagaatcc gatcgcgtcc gcgagtggct
       61 ggaagacctc aaacccacgg acttcgaagc cccgtaaagc cggaacgagt cgggatcaga
      121 gcgagagctc tgcggtcgcc aaatcgacct cgacggccgg agactcgtcg tgatcgcggt
      181 ccttgtgcag tttgacggac acgattttcg acaccccggc ctcttccatc gtgactccgt
      241 acaggacgtc ggcctgttcc atggttcgcc gattgtgggt gacgaccagg aactgcgagt
      301 gcgcggtcat gggacgaagg gcgcgcgtga agcggcggac gttctcttca tccagcggcg
      361 cgtcgatttc gtccatgacg cagaacgggc tgggatgaat caggaagctt gcaaacaaca
      421 gagaaatcgc cgtcagcgct ttttcgccgc cggataacag ggagatgtgg cggagtttct
      481 tgcccggagg ctgggccacg atctcaatgc cggactccag cggattggtc tcgtccacta
      541 ggtgaagggc ggcctggccc ccttcgaaga acgacgcaaa gacctccccg aaccgagtgt
      601 tgagggactc gaacgcctcg agaaaaaggg tcttggtggt ggcgttgatc ttggcgatgg
      661 cggcgcggag attgtcgacg gactgcacga gatcgccttc ctgggtggcc aagaacccgc
      721 cgcgctcctc caattctcgg tgctcgtcga tggccgcgag gttcacgccc ccgatttgca
      781 ccagcgtagc ccggaggtcg gtgatcgccg cgtcgaactg atcggcttca caggtcggcg
      841 gcgggagcga gtccgcgtca aaggcctcca gctcgacctg atagcggtcg cgaatatccg
      901 agagttgctg gccgatccgg agcctgattt cggtgagcga caccgtgtgc gcgtggaccc
      961 gttcctggac ggcgtccgcg tgacgacgcg cggctttcga ctgatcttcc atctccttga
     1021 cgcggtgcga cacgtcggcg cgagcggcgt ccaactccgc gacgcgctgc cgcaagactt
     1081 cgacgtggcg gtccagcaca gcgatctcat cgacgatccg cacgcgctca agcgtcgcgg
     1141 ccgcgatcct cgcgtcgaac tgggtgcggg aggccccctt cgaatccatc tggcgccccg
     1201 cttcatccag ccgttccgcg atcgcccgaa gcgtcgccgc cgcgtgctca cgcttttcgc
     1261 ggtcggccgc gagcgcgagg cggagctcgg tggccgcggt ctgttcggtc gcgagcgcgg
     1321 acttgagctg cgcggcggcc tcatactgac cgagcaacgc ggcctcatcg cgcactttgg
     1381 aggcctcgcc agcggcgagc gcggcgtggt gattggcgac ctcctgatcc attgccgcga
     1441 gttcgctgac cacggcggac tcttcagtag ccagtagggc cagccggcga tcgagctgga
     1501 gtagttcctg cgccagcgct tccgcggcct tttgatcgtg gaggaggcgg agctcgccgt
     1561 ctcgaacctc ggcatcgagc gcttcgagaa ccgagcgatc agccgagagg gcgtcccgca
     1621 gcgacgcggt cgaagcctcc agcggtctga gcgctgtttc ggtgttcgcg atttcctgtt
     1681 caagcgtctt gatctgacgc ttccgctcaa gcagggtgag gctcgcgttc gaggctttcc
     1741 cgccgctgac cacgccggat ggctgaaaca catcgccgga gacggagacc cacgtcatgc
     1801 cggattggtc ggcctcccac aatcgacgcg cgaccgcccg gtcttccacg atcaccacgc
     1861 gcgcgagaag cgtgtcccgg agcgactcaa acccgtcgcg gcacccgacc aggcgagagg
     1921 cgaagccgcg gacgccgtcc agccggagcg tctccgcgtc ggaatcgacc gacgggacgg
     1981 cggcccgggg cgccttgggg acgaacaccg cgcgaccggc gtcgagacgg tccaacagcg
     2041 acaaggctga gacaatttgt tcgtgatctt ccgcgaccac gccttgcagt cgttcagtca
     2101 acacggcttc aacggctcgc tcgtactccg ccgggatatc caggacgtcg gccaccacgc
     2161 cgagcagccc tgcgaccggc gcggtggagg tgtctcccag cagcacgcgc cgcacgccct
     2221 cgtcgtaccc gacgagatct cgctgcaggg cgtgcagcga gtccagcgtc gcgcgtaatc
     2281 gcaccaactc gtcgcgccgc ccggacaggg ccgcttctcc ctcctggagg gcggcctgcc
     2341 gggtggccga aacctcccgc aaagccgctc gttcagcatc ccgcgttgcg agcgacgttg
     2401 cttggacctg gtgggaggtc tcgagctcgt gatggactcc acgtttgtgg gccatctcgg
     2461 tctggacggt tccgatatcc tcgcgatatc gcgagcgcct ccgctcaagc tcctcgcggc
     2521 ggaccgagag actggccaag gcgttggtcg cggtcgccac gctggacgcg gcctcgaaca
     2581 cgcgatggcg cgtttgctcg atttcggcgg ccgcctgggc gagctggtgt tccaggtggc
     2641 ggattgcgcg ttcccgatca gcgaccagag cggtccgctc ctcaatggcg gcgtccaagg
     2701 acgcggcctg ttcgttcaac cgcgtctgct cctcgccgcg cgcggcgagc gcgcgcgaaa
     2761 gctcttcgat ttccgtggcg agagtcgctt gttgggtgtg ccattcctcg acctgcgcgg
     2821 caagcacgtc gatccggtga tgtccgtcgc gtgcggcggt ttcgcgggac gctaactcgg
     2881 cacgcgcttc gagcaggcgc tgctcgagct cggcgccgtc gaccttgagc gattcagtgg
     2941 ccgcgtcgag ggcgctcaat tgcgccagcg cgccggcggc ctcggcgcgt tcgcgctcca
     3001 agtctgccgc caacgtcgcg gcttggtcga accattcccg ataacggatc gcggcggcgc
     3061 gcagctccaa cgcccggagt tgatcggcgg cctggcggta gcgttccgcc ttccggacct
     3121 gccggtccag cgacgcgagc tgccgtttga cctcgccgat aatgtcgcga acgcggagca
     3181 ggttctgctc ggtggactcc aatttgcgga gcgcttcggc ctttcgaagc ttgtatttgg
     3241 tgatgccggc ggtttcctcc accagcgcgc gccgttcgat cggcgacgcg gtgacgattt
     3301 cggcgacctt tccctgttcg aggacggtat gtcccttgat cccggccccg gattcaatga
     3361 ggaggtcgcg gatatctcgt agccggcagg ggacgcgatt gatcaggtac tcgctgtcgc
     3421 ccgatcggta caatcgtcgc gtcaatgtaa cttcgctgta cgagccagcc gtgccctcga
     3481 tctcaccagg cctcacgccg cccagagtga gcgacacctc ggccagtccc gtggccgtgc
     3541 gtcgggtgtt gccgttgaac aagacatctt ccatcttgtc gccgcgcagg gtcttgggcg
     3601 attgctctcc caaacaccag agtatggcgt cggcgatgtt acttttcccg cacccgttcg
     3661 gccccacaat ggcggtgatg ccgggctgga aggagagaac ggtgcgttcg ccgaacgact
     3721 tgaacccaag gatttccagt cgtgttaatc gcattcagcg ctcgctatgg tgacggaagt
     3781 ctcgaaggaa ccggggcgat ctataacata ggctttggcg caatgtaaag cagaaaaacg
     3841 acaatttcag gcggacttgt gtcgcttgaa atcgagcgaa gaccgtcgcg acctcactga
     3901 gatttctggt atagtacgcc cgcgatggat cacgatccaa aagccgctga cgccggacat
     3961 gtggccgtaa cttgtccgtg ttgtcgagcc gaactcaccg tggatgccgc aaccggagcg
     4021 gtcatttggc atcagccgga atctccgacg ccccagcggt ccctcaatga cctggtcaag
     4081 gacctggaag tcaagcggcg ggaggtcgcg gatcgattcg aacgggaacg gcggtccctc
     4141 ggtgaccgca gccgcgtcct cgatgaaaag gtcaaagagt ccctcaagcg agtggacccc
     4201 aacaccccgc cgccgatccg cccgatcgac ctagactgaa ccgataacac catcgcgtct
     4261 cgcgcccgtg atttcttgcc ggtgcttggg gcgtgtgtta tctttcctcc gctcggagca
     4321 cggcatgctg ccccagtggg aaatcgtcgc ggcgatcggg gtattcggcg ccctgatcgg
     4381 aagcttcctc aacgtctgca tccaccggct accgctgggc gagtctctgg tgagcccccg
     4441 ctcacggtgt cccggctgtc ggaagccggt gcgcgcctac gacaatatcc caatcgtctc
     4501 ctttgtgttg ctcggcggcc ggtgtcgttc gtgtcggcaa cgaatcgcca ttcgttatcc
     4561 cgcggtcgag gcgctcaacg ccgcaggcta tatttttata tggtatcggt tcggcgccac
     4621 gccggacgcc gtggtgtttg gcgcgttcta ttccgcgttg ttggtgatca cgtttatcga
     4681 ttgggaccac cagatcatcc cgaaccggat cacgtacccc ggcatcgcca tcggcctgct
     4741 ctcggcggcg accgtattgg ccgggagctt cgtggacgcg ctgttgggag cgatcttggg
     4801 gggaggcctt ctatatggtg tcgccgtggg cagcgaatgg gtgctcaaga aagaagggat
     4861 gggattcggc gacgtcaaac tgctcgccat gatcggcgca tttctgggct ggagggcggt
     4921 attggtcacg atctttgtgg gcgcgcttgc cggctcggtc atcgggctca cattgatggc
     4981 gttgcgaatc aaaaagcgcg gggaccccat cccgttcggt ccatttctgg cgctcggagc
     5041 ttgcgccgcg ctgtttgccg ggccggaatt gatcgcctgg tatctgcggc tgggcacgta
     5101 gcctcgggta tggcgaaccg atttggagga gcccggtctc tcgtctcgtt gagtgtattc
     5161 ggcgcgctgc tgttgaccgc tctgggagtc cgccggcatg tgttcccgca cccggtcgct
     5221 gaacgcgccg tgtccgccgt ggatctggtc attgtcgtga gcctgatcgc gctcgcgttt
     5281 gcggggttca tgttccgccg ccggagccgc ttctccgtcc agcccgctca ccaggaggac
     5341 gatcgcgcgg gcttcgtcat gcacaccttc caggaggtcc tccgccagct caaagagaag
     5401 gaagcggaac tggaacactt gcgggctcga gcggtggcgc gtgccgaaga cgtcgagagt
     5461 taccacgaaa atatccttcg cagcatcgca agcggagtga tcacctgcga tcctgcggga
     5521 cgcatcacca catttaacgc ggcggcggaa cggattctgg gtcttgaggc ggcgcacgtc
     5581 gtcggacggt cttgcgacga gatctttgga aacgacagcc ctatcccagg catggtccac
     5641 gggtcgttgg aagcgcttgc gccgatttcg cgccgtgaat ggcatttttg ccgacgcgac
     5701 gcgcatcggg tctgtgtcgg gctctcgagc gcgctgctca gagaccggaa tgatcagctc
     5761 atcggcgcgg cactggtctt caccgacctc accgagatca agcggctgga ggagcggatc
     5821 gagtccgaac gccggctggc aatgctcggg gagatgtcag cggcaatcgc ccacgaattc
     5881 cgcaattata tggggacgat cgtcggatgg gccaaactct tgaccaaaca ggtcgaggcc
     5941 gggacccagg cagggtcgat gatcgcggcc attcggcgcg agctgggcat gatgcaacgc
     6001 ctcatcgatg atctgctgtc gtttggccga cggctggagc cccagaccga gcgtgtcgac
     6061 cttcgcgagc tggtcgccca agggatgagc ctcccgccgg atcgacccga tgtgcgcgtg
     6121 gaaacggact gggcccacgg ggcggcgacg acggcgcact gggacccgac gttgatgcgg
     6181 caggtgctca agaacttggc gcagaac
//
LOCUS       DBNGTT010000250         3345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1716393, whole genome shotgun sequence.
ACCESSION   DBNGTT010000250 DBNGTT010000000
VERSION     DBNGTT010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1716393"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC16BC_05595"
     CDS             <1..355
                     /locus_tag="AC16BC_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3241127.1"
                     /translation="RRTFMIKGRDLISGIPRTLVIDDAEIREALAEPIGAIVNAIKVA
                     LENTPSELAGDIIDRGIVLTGGGSLLRGMDVRLREETNLPIITVDNPLTTVVLGTGKA
                     LDQIDLLQRVSILAQ"
     gene            401..1210
                     /gene="mreC"
                     /locus_tag="AC16BC_05600"
     CDS             401..1210
                     /gene="mreC"
                     /locus_tag="AC16BC_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3241128.1"
                     /translation="MWLVALGIMVVVAAAILPDWQRRTVPGVVRPLTAPFVFLDNALS
                     GLVGSVSDAWSRYVALTAIQDSNRLLREDVDRLTRERDALVELAAENRRLAALLDFRA
                     AITIPTHGARVIGRSPSRWYQSIIIDRGSDDGAVIDMGAAIAGGVVGTIIKTLPSASM
                     VLLITDRQSAVPVVVQRTREQGIVEGTIAGRLRLKYLPPSSEIREGDVLLTSGLTAPF
                     PKGLLVGTVTRVDRPAGALFPEVEVFPSANLSAVEEIVVFEPSRDSEHVDP"
     gene            1197..1781
                     /locus_tag="AC16BC_05605"
     CDS             1197..1781
                     /locus_tag="AC16BC_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR3241129.1"
                     /translation="MWIRERVERPGASDRRRAAWLVVFSLVVPFQSVLAPIGAIGGVV
                     PDLPFVIVVLFALGHRRITSAAAGGVVGALVDLFSSGSGSFHLAAYTLLAVLASSIGR
                     LTTNVRALTAVTVVAFCSVAAGVGYAVTGAPVERADDMTRWMGEVLGPQVVYNTIVGW
                     CLFAVWRWWNPLSRDAVGERDELFSSGRFQGFIR"
     gene            1738..>3345
                     /gene="mrdA"
                     /locus_tag="AC16BC_05610"
     CDS             1738..>3345
                     /gene="mrdA"
                     /locus_tag="AC16BC_05610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR3241130.1"
                     /translation="MSSSPQDDFKDLYDRLTWGVFAIIALLGMLVLRAWYLQVVAGDT
                     YRELAETNRVRTVSVLPQRGLIFDRHGQLLVNNTPGFTAYVVTEDAPNPLDPLLERLA
                     SYLDLSEDEIRERVAARKNPGSYTAIPIKPHLSLKEVALIEAHRFELPGVRIEVEAQR
                     NYPHGSWASHLLGYVNEVSAAQRSSEDFANLPLGIQVGQAGVELAYDSVLRGVPGEKG
                     IEVNALGHERQVVRQTPATQGDDLFLTIDAGVQGVAEEALAGKSGVVVALDPTTGEVL
                     AMVSHPDVDPNTLSGGVTASRWAELLEDPGRPLNNRAIQGQYPPGSTFKIVVAAAALE
                     RRAVTAQTQKACAGGKFFGNRTFRDWKAGGHGIVDLHRALVESCDVYFYEVGDQLGVD
                     AIAEFARAFGLGEPTGIRLASEKKGLIPSTEWKLASRREPWYPGETLSVAIGQGYVSA
                     TPLQLTVMIGTVATGGDRYQPHYVREIRHRDGSITPAGEAHNEHVAVSPKTFAVLREA
                     LKGVVLETSGTGRSAKSDVADIAGKTGT"
BASE COUNT          529 a         1011 c         1202 g          603 t
ORIGIN      
        1 tcgcagaacc ttcatgatca agggacggga tctgatttcg ggcattcctc gcacgctcgt
       61 catcgacgac gcggagatcc gggaggcgtt ggccgaaccg atcggcgcca tcgtcaacgc
      121 catcaaggtc gcgctggaga acacgccgtc ggaactggcc ggggacatca tcgaccgtgg
      181 gatcgtgttg acgggcggcg ggtcgttgct gcggggcatg gacgtccggc tgcgcgaaga
      241 aaccaacctg ccgatcatca cggtggacaa ccccctgacg acggtggtgc tcggcaccgg
      301 gaaggcgctg gaccagatcg acctgttgca gcgcgtctcg attctcgctc aataagcatc
      361 ggcacgcggt atggcctacc ccgcctcggg atctaagcgc gtgtggttgg ttgcgctcgg
      421 gatcatggtg gtcgtggctg cagccatcct cccggactgg cagcgacgga ccgtgccggg
      481 ggtggtccgt ccgctgacgg cgccgttcgt cttcctcgac aacgcgctgt ccgggctggt
      541 gggctcggtg agcgacgcgt ggtcccgata tgtggcgctg accgccatcc aggattcgaa
      601 ccgtctgctg cgggaagacg tggaccgcct gacccgcgaa cgggacgcgc tggtcgaact
      661 ggcggcggag aaccgcaggc tggccgcgct tctggacttc cgcgccgcga tcacgattcc
      721 cacgcacggg gcgcgggtga tcggccggag tccctcgcgc tggtatcagt ccatcatcat
      781 cgatcgcgga agcgacgacg gcgcggtgat cgacatgggg gccgcgattg ccggcggcgt
      841 ggtcgggacg atcatcaaga ccctgccctc cgcgtcgatg gtgttattga tcacggaccg
      901 gcaaagtgcg gtgccggtgg tcgtccagcg tacccgcgag caggggatcg tcgaagggac
      961 catcgccgga cggctccggt tgaaatatct gccgccgtca tccgagattc gcgaggggga
     1021 cgtgctgttg acgtcggggc tgacggcacc gtttccaaaa ggactcctcg ttgggaccgt
     1081 cacgcgcgtg gatcgccccg ccggcgccct gtttcccgag gtcgaagtgt tcccgtccgc
     1141 gaatctgtcg gccgtcgaag agatcgtggt attcgaaccg tcccgggact ccgaacatgt
     1201 ggatccgtga gcgggtagag cgtcccgggg cgtcggaccg tcgccgggcc gcgtggctcg
     1261 tcgtgttctc gctcgtggtt ccgttccaga gcgtgttggc gccgatcggg gcgattgggg
     1321 gcgtggtgcc cgacctgccg ttcgtgatcg tcgtgttgtt cgccctcggt catcgccgaa
     1381 tcacgagcgc cgcggccggc ggggtggtgg gcgctttggt ggacctgttc tcgtcgggct
     1441 ccgggtcctt tcacctggcg gcctatacac tgctggcggt gctggcctcg tcgattggcc
     1501 ggctgaccac caacgtgagg gcgttgaccg cggtgaccgt ggtcgcattc tgctcggtgg
     1561 cggcgggggt cgggtacgcg gtgacgggcg cgccggtcga gcgcgccgat gacatgacgc
     1621 ggtggatggg cgaggtactc gggccgcaag tggtgtataa cacgatcgtt ggttggtgtt
     1681 tgttcgcggt ctggcgatgg tggaatccgc tgtctcgcga cgcggttggg gaacgggatg
     1741 agctcttctc ctcaggacga tttcaaggat ttatacgata ggctgacctg gggggtcttc
     1801 gcgatcatcg cgctgctggg gatgctggtg ctccgcgcgt ggtaccttca ggtggtcgcg
     1861 ggggacacct atcgagagct ggccgaaacc aaccgcgttc gaacggtgag cgtgctcccg
     1921 cagcgggggc tgattttcga ccgccacggc cagctcctgg tgaacaacac ccctgggttt
     1981 acggcctatg tggtgaccga agatgcgccc aatcccctcg atccgttgct cgagcgcctg
     2041 gcatcgtacc ttgatctgtc ggaagacgag atccgcgaac gagtggcggc ccgcaagaac
     2101 cccggctcct ataccgcgat tcccatcaag ccgcacctga gcctgaagga ggtggcgttg
     2161 atcgaagccc accgcttcga gctccccggc gttcgcatcg aggtggaagc gcagcggaac
     2221 tacccgcacg gttcgtgggc gtcgcacctc ctggggtacg tgaacgaagt gtcggcggcg
     2281 cagcgctcgt ccgaggactt cgcgaacctc cctttgggca ttcaagtggg ccaggccggg
     2341 gtcgagctgg cctacgacag cgtcctgcgg ggcgtgccgg gggagaaggg catcgaagtc
     2401 aacgcgttgg ggcacgagcg tcaggtggtg cgccagacac cggccacgca gggcgacgac
     2461 ctcttcctga cgatcgacgc gggcgtgcag ggcgtcgcgg aggaggcgct tgccggcaaa
     2521 tcgggcgtcg tggtcgcgtt ggatcccacg accggggagg tgctcgccat ggtgagccat
     2581 ccggatgtcg atcccaatac gctgtccggt ggggtgaccg cgtcgcgttg ggccgaactg
     2641 ctggaggatc ccggtcgccc gttgaacaat cgggcgatcc aagggcagta tcctccggga
     2701 tcaaccttca agatcgtggt cgcggcggcg gcgctcgaac gccgcgccgt gacggcgcag
     2761 acgcagaagg cgtgcgccgg cggcaaattc ttcggaaacc ggacgttccg cgactggaag
     2821 gccggcggac acggcatcgt ggatctccac cgggcgctcg tcgaatcgtg cgacgtctat
     2881 ttttacgaag tgggcgatca actcggcgtg gatgcgatcg cggagtttgc gcgtgcgttc
     2941 gggttgggcg aaccgactgg gatccggttg gcgtccgaga agaaggggtt gatcccgtcc
     3001 accgaatgga aactggcgag ccgccgggag ccgtggtacc cgggcgagac gttgtccgtg
     3061 gccatcggtc aagggtacgt ctcggccacg ccgctgcaat tgacggtgat gatcgggacc
     3121 gtcgcgaccg gcggcgatcg ctaccaaccc cactatgtcc gcgagattcg ccatcgcgac
     3181 ggctcgatca ccccggccgg cgaggcgcac aacgagcacg tggcggtcag tccgaaaacg
     3241 ttcgccgttt tgcgggaagc gctcaagggc gtggtgctcg aaaccagcgg aaccgggcgc
     3301 tccgcgaaat cggacgtggc ggacatcgcg ggcaagaccg gaacg
//
LOCUS       DBNGTT010000251         2539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3121751, whole genome shotgun sequence.
ACCESSION   DBNGTT010000251 DBNGTT010000000
VERSION     DBNGTT010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3121751"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2537)
                     /locus_tag="AC16BC_05615"
     CDS             complement(<1..2537)
                     /locus_tag="AC16BC_05615"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIR3241131.1"
                     /translation="MEALGYSVLHGPEIAAGEPGAERSDPNYRDVVLERRLRQALVAL
                     NPDLPADALDDAFRKLTRTDAPSLLERNRAVHRMLVDGVTVEYRRPDGSIAGAQARVF
                     DFDRPDQNDWLAVNQFTVAEGQHIRRPDIILFVNGLPLAVIELKNPADEDATVWTAFK
                     QLQTYQAELSTLFAYNAALLVSDGVQARIGSLGAGREWFKPWRTIGGREDAPPSLAEL
                     QVVLEGVFDKRRFLNLVRHFLVFEDLGGGALAKKMAGYHQFHAVNVAVDETLRAAKLA
                     APDQIGGYTGRYAAGHQSGGEPGDRRVGVVWHTQGSGKSLSMAFYAGRVILHPAMANP
                     TIVVLTDRNDLDDQLFGTFARCQDLLRQAPVQAENRADLREKLRVAGGGVVFTTIQKF
                     FPVGQVGQVGPLSERRNIVVIADEAHRSQYDFIDGFARHMRDALPHASFIGFTGTPIE
                     VTDKNTRAVFGDYISVYDIQRAVLDRATVPIYYESRLAKLALKDSERPKIDPEFEEAT
                     EGEEVEQREKLKTKWAQLEAIVGSARRIQLIARDLVDHFENRLAALDGKAMIVCMSRR
                     ICVELYKEIVALRPQWQGDADDQGTIKVVMTGSASDPTDWQPHIRNKARREALAQRFR
                     DANDPFKIVVVRDMWLTGFDAPSLHTMYVDKPMRGHGLMQAIARVNRVFKDKPGGLVV
                     DYLGLAAELKQALATYTESGGTGKTAIDQAEAVAVMLEKYEVCYGLFYGFDWTKWVSG
                     TPQERLGVLPAAQEHILALNDGKPRLLRAVTELSQAFALAVPHEEALRIRDDVGFFQA
                     VRAVLAKNVPGERKTDEELDHAIRQIVSKAVASDQVVDIFTAAGLKKP"
BASE COUNT          441 a          874 c          762 g          462 t
ORIGIN      
        1 ggcttcttga gccccgcggc cgtaaagatg tccaccacct ggtccgaggc caccgccttc
       61 gagacgatct gccggatcgc gtggtcgagt tcctcgtcgg tcttgcgctc gcccggcacg
      121 ttcttggcga gcaccgcgcg cacggcctgg aagaacccca catcatctcg aatgcggagc
      181 gcctcttcgt gcggcaccgc gagggcaaag gcctgcgaca actcggtaac tgcccggagc
      241 aaacggggtt tgccatcgtt caaggccaga atgtgctcct gtgcggcggg gaggacgccc
      301 agtcgctcct gcggcgtgcc tgagacccat ttagtccagt caaagccgta gaacaacccg
      361 tagcacacct cgtacttctc cagcatcacg gcgacagcct cggcctgatc gatcgcggtc
      421 tttccggtcc cgccgctttc cgtgtacgtg gccagcgcct gcttcagctc ggcggcaagt
      481 cccaagtagt ccaccaccag cccgcccggc ttgtccttga atacgcggtt cacgcgcgcg
      541 atggcctgca tgagcccgtg gccgcgcatg ggcttgtcca cgtacatggt gtgcaagctg
      601 ggcgcgtcaa agcccgtgag ccacatgtcg cgcacgacca cgatcttgaa cggatcgttg
      661 gcgtcgcgga agcgctgggc caacgcctca cgtcgcgcct tgttgcggat gtgcggttgc
      721 cagtcggtgg gatcggacgc cgagcccgtc atcaccacct tgatcgtgcc ctggtcatcc
      781 gcgtcaccct gccactgcgg gcgcaaggcc acgatctctt tgtacagctc cacgcagatg
      841 cggcggctca tgcacacgat catggccttg ccgtccaggg cggcgagccg gttctcgaaa
      901 tgatcgacca gatcgcgggc gatcaactga atccggcgtg ccgagcccac aatggcttcg
      961 agctgcgccc acttggtctt cagcttttcc cgctgctcga cctcctcgcc ctcggtggct
     1021 tcctcgaact ccggatcgat cttgggacgc tcggagtcct tcagggcgag cttggcgagc
     1081 cggctttcgt agtagatggg cacggtcgcg cgatccagca ccgcgcgttg gatgtcgtag
     1141 acgctgatgt agtcgccgaa caccgcgcgg gtgttcttgt ccgtgacctc gattggcgtg
     1201 ccggtaaaac cgatgaacga ggcatggggc aaggcatcgc gcatgtgccg ggcaaagccg
     1261 tcgataaagt cgtactggct gcggtgcgcc tcgtccgcga tcaccacaat gttgcgccgc
     1321 tccgataagg gtccgacctg tccgacttgt ccgaccggaa agaatttctg gatcgtggtg
     1381 aaaaccaccc cacccccggc cacacgcagt ttctcgcgca aatcggctcg gttctcggcc
     1441 tgcaccggcg cttgccgcaa caggtcttgg cagcgcgcga acgtgccgaa gagctgatcg
     1501 tccaaatcat tgcgatcggt cagcaccacg atcgtggggt tggccatggc cgggtgcaaa
     1561 atcacccgcc cggcgtaaaa cgccatgctc aggctcttgc ccgatccttg cgtgtgccag
     1621 accacgccca cgcgccggtc acctggttcg ccaccggatt gatgtcccgc cgcataacgt
     1681 ccggtgtacc cgccgatttg atccggagcc gccagtttgg ccgcgcgcaa tgtctcatcc
     1741 accgccacgt tcaccgcatg gaactggtga taccccgcca tcttcttggc cagcgccccg
     1801 ccacccaggt cctcgaacac caggaagtgc cgaaccaagt tgagaaaccg ccgcttgtcg
     1861 aacacgcctt caagaacaac ctgcaactcg gccagactcg gcggcgcatc ctcgcgtccg
     1921 ccaatcgtgc gccacggctt gaaccactcg cgcccagccc cgagcgaccc gatgcgcgcc
     1981 tgcacgccgt cggacaccag gagtgccgcg ttgtaggcaa agagcgtgga gagttcggct
     2041 tgataggtct gaagctgctt gaacgcggtc cagaccgtgg catcctcgtc agccggattc
     2101 ttgagttcga tcaccgccag cggcagcccg ttgacgaaca agatgatgtc aggccgccga
     2161 atgtgctgcc cttcagccac cgtgaactga ttgaccgcga gccagtcgtt ctgatccggc
     2221 ctgtcaaaat cgaacacgcg cgcctgcgct ccggcaatgg agccgtccgg acggcgatac
     2281 tccaccgtca cgccatcgac cagcatccgg tgcaccgcgc ggttgcgttc caagagcgag
     2341 ggcgcatcgg ttcgcgtgag cttgcgaaac gcgtcgtcga gcgcgtcggc cggcaggtcc
     2401 gggttgagcg caaccagcgc ttggcgcaag cggcgctcca agaccacgtc gcggtagttc
     2461 gggtcgctgc gctccgcccc cggctcgcct gccgcgatct ccgggccgtg cagcacggaa
     2521 tagccaagcg cctccagcc
//
LOCUS       DBNGTT010000252         4385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2610987, whole genome shotgun sequence.
ACCESSION   DBNGTT010000252 DBNGTT010000000
VERSION     DBNGTT010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2610987"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="urtE"
                     /locus_tag="AC16BC_05620"
     CDS             <1..600
                     /gene="urtE"
                     /locus_tag="AC16BC_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020294146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HIR3241132.1"
                     /translation="AIMGRNGMGKTTLFKTLMGILPVRSGAIEVGGVDVTTCDSYERV
                     ARGIAYVPQGRMIFPALTVAENIKTGLGKTAAGRVPEDIYSLFPVLFDMSHRKGGNLS
                     GGQQQQLAIARALATNPKVLLLDEPTEGIQPSIIKDIAKTLKEIRDLRGLTIVVSEQV
                     LSFTMAIADRLFVIDRGRFVRHDQRANVDVKTVTQYLAV"
     gene            655..1884
                     /gene="fmdA"
                     /locus_tag="AC16BC_05625"
     CDS             655..1884
                     /gene="fmdA"
                     /locus_tag="AC16BC_05625"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HIR3241133.1"
                     /translation="MAKTLIEIDLSSSPYKNEMIHNRWHPDIPMVATVKPGADFVLQT
                     YDWTGGFIKNNNSADDVRDIDLSIVHFLSGPIGVEGAEPGDLLVVDLLDVGARPDSLW
                     GFNGFFSKKNGGGFLTEHFPLAQKSIWDISGLYTTSRHVPGVRFAGLIHPGLIGCLPS
                     QELLNTWNAREASLIATNPTRVPPLAAPPFANTAHAGRAKGAVKDKIAAEGARTVPPR
                     EHGGNCDIKDLSRGSRIYFPVYVPGAGLSMGDLHFSQGDGEITFCGAIEMAGWLHLGV
                     DLIKGGMAKYGIKNPIFKPSPITPTYNDYLIFEGISVDEQGKQHYLDVHIAYRQACLN
                     AIEYLTKFGYTRAQAYAILGTAPVQGHISGVVDIPNACATLWLPTEIFDFDVRPNASG
                     PTKMVTSQADIPMSMDV"
     gene            1956..2225
                     /locus_tag="AC16BC_05630"
     CDS             1956..2225
                     /locus_tag="AC16BC_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3241134.1"
                     /translation="MVQYQEPQPCPACHVLAPRIVVTAPACSVVPGSTRKAHALNERS
                     AHEPKRTMPGAAGHDSHRARRASRAPGPAGGTRGTAGTRPWMIGH"
     gene            complement(2263..3150)
                     /locus_tag="AC16BC_05635"
     CDS             complement(2263..3150)
                     /locus_tag="AC16BC_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HIR3241135.1"
                     /translation="MAIRGVIPGGVGVIGESTDPRDGNGDRVRTTDGSMELLFERVGR
                     RTVLRRNVSTAPLRVPPPVDLDGCAYVTLLNVSGGIVGGDRLRHDLTVRAGAHALATT
                     QSATKVYRCAGVEARSDVRVVVETGGVLEWIPDPVIPFAGARFAQSLVVELAPGAVAI
                     VSDVWAAGRIARGERWAFARIANHLLVTLNGRPSVQEGFVIAAQAAAALEHWPYLASW
                     YVVGGGTTDWCGLAESISTRLAECEPEVYGGASTLDHGVAVRFVASSSDTVSRASTRV
                     WDQIRRQTLGLAVPLLRKA"
     gene            complement(3154..3771)
                     /gene="ureG"
                     /locus_tag="AC16BC_05640"
     CDS             complement(3154..3771)
                     /gene="ureG"
                     /locus_tag="AC16BC_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HIR3241136.1"
                     /translation="MNYPVIIGVGGPVGSGKTALVEALCRRLVHDHRVAVITNDIFTR
                     EDAEFLTRRGVLAADRIIGVETGGCPHTAIREDASHNQEALQQLVARFPDLDLVFIES
                     GGDNLAATFSPELVDATIYVIDVAAGDKIPRKGGPGITRSDLLVINKIDLAPHVGADL
                     GVMDRDAGRMRGDRPYLFSNLKSGQGLDGVETWVQRRVGSPVSVR"
     gene            complement(3787..>4385)
                     /locus_tag="AC16BC_05645"
     CDS             complement(3787..>4385)
                     /locus_tag="AC16BC_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013858.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HIR3241137.1"
                     /translation="VADGRVRSEDDVAAYLGHLLRDGVATCDAVAVAAAHRALEQGDV
                     ASLVDVDWNLEAFKFAREAREGSRVMGRRYLEWGVLCAATEGLRQYEAAIRHGDAPGH
                     AATALGSVLAACGWSRQDAIGAALYQTAVGWVSAAIRLMPVGQRGGQRVLASLLPVIE
                     SAAREAESGDVGSMRSWTPMHDIRMIRHERQPTRLFRS"
BASE COUNT          804 a         1544 c         1346 g          691 t
ORIGIN      
        1 gcgatcatgg ggcgcaacgg catgggcaaa accacgctgt ttaagacctt gatggggatc
       61 ttgccggtcc ggtcgggcgc catagaggtc gggggcgtgg acgtgaccac gtgcgacagc
      121 tacgagcggg tcgcgcgcgg gatcgcctat gtgccgcagg gccggatgat cttcccggcg
      181 ttgaccgttg ccgagaacat caagacgggt ctgggcaaaa ccgcggccgg tcgcgtcccc
      241 gaggacatct acagcctgtt cccggtgctg ttcgacatgt cgcatcgcaa gggcggcaat
      301 ctctcgggcg ggcaacagca gcaactcgcg atcgcccggg cgctcgccac gaatccgaag
      361 gtcctgttgt tggacgagcc gaccgaaggt atccagccat cgatcatcaa agacatcgcc
      421 aagaccttga aggagatccg cgacctacgc gggctcacca tcgtcgtgtc cgagcaggtg
      481 ctcagcttca cgatggcgat tgcggatcgc ctgttcgtga tcgaccgggg ccgcttcgtc
      541 cgtcatgacc aacgcgccaa cgtggacgtg aaaaccgtaa cccagtatct ggcggtgtga
      601 ggcgtgtggc cgtccaccga cgagatccgt cactgaggag agaaggagag actcatggcc
      661 aagaccctga tcgagatcga cctgtcatcg tcgccgtaca agaacgagat gatccacaac
      721 cggtggcacc cggatattcc catggtcgcg accgtgaaac ccggcgccga ctttgtcctc
      781 cagacgtacg actggaccgg cggcttcatc aagaacaaca attcggcgga tgacgtgcgt
      841 gacatcgacc tgtcgatcgt gcacttcctg agcggaccga tcggagtgga aggcgccgag
      901 cccggcgatc tcctggtggt ggacttgttg gatgtcggcg cgcgtcccga cagtttgtgg
      961 gggttcaacg ggttcttttc caagaaaaac ggcggcggct tcctcaccga gcacttcccg
     1021 ctggcgcaaa aatcgatttg ggatatttcc ggtctctaca ccacgtcgcg gcacgtgccg
     1081 ggcgtgcggt tcgccggact catccatccg gggttgatcg ggtgcctgcc gtcgcaggag
     1141 ctgctcaaca cctggaacgc gcgggaagcg tctctcatcg ccaccaaccc cacgcgcgtg
     1201 cctccgctcg ccgcgccgcc gttcgctaac acagcgcacg cgggccgggc caagggcgcg
     1261 gtcaaggaca agatcgcggc cgagggcgcg cgcacggtgc cgccgcgcga gcacggcggc
     1321 aactgcgaca tcaaagactt gtcgcgcggc tcgcgtatct atttcccggt atacgtgccg
     1381 ggcgccgggc tctccatggg cgatttgcac ttcagccaag gcgacggcga gatcacgttc
     1441 tgcggcgcca ttgaaatggc ggggtggctg cacctgggcg tggacctgat caagggcggc
     1501 atggccaagt acggaattaa gaatccgatc ttcaagccca gcccgatcac gcccacctac
     1561 aacgactatc tgatcttcga gggcatctcg gtggatgagc agggcaagca acactacctg
     1621 gacgtgcaca tcgcctaccg gcaggcgtgc ctcaacgcca tcgagtatct caccaagttc
     1681 gggtacaccc gcgcccaggc ctacgcgatc ttgggcaccg cgccggtgca agggcacatc
     1741 agcggcgtgg tggacattcc caatgcgtgc gccacgttgt ggctgcccac ggagattttc
     1801 gatttcgacg tccggccgaa cgccagcggg ccgaccaaga tggtcacgag ccaagcggat
     1861 atcccgatgt cgatggacgt ttaacgggag gggggccgat gccgttctac gaatacgact
     1921 gcgccgggtg cggcgtgttt tcagccttca agcccatggt ccagtaccaa gagccgcagc
     1981 cctgcccggc atgccacgtg ctcgcgcccc ggatcgtggt gaccgcgccc gcgtgctccg
     2041 tggtgcccgg ctccacgcgg aaggcgcacg cgctcaacga gcgcagcgcc cacgaaccga
     2101 aaagaaccat gccgggcgcc gcgggccatg actcccaccg cgcccggcgc gcgagccgtg
     2161 cccctggccc agccggcgga accaggggga cggccggaac ccggccctgg atgattggcc
     2221 attgatccgc cgggagggtc tggagcctgc cgcccttccc ggctaggctt tgcggagcag
     2281 ggggaccgcg agaccgagcg tctgccggcg aatctgatcc cacacccgag tcgaggcacg
     2341 gctgacggta tcggaggacg acgcgacaaa ccgcacagcc accccgtggt ccagggtgga
     2401 ggctcccccg tacacttctg gctcacactc cgcgagcctc gtgctgatcg attccgccag
     2461 gccgcaccag tctgtagttc caccacccac gacgtaccac gacgccaggt acggccagtg
     2521 ttcgagcgcc gccgcagcct gcgcggcaat gacaaaaccc tcctgaacgc tcggccgccc
     2581 gttgagggtc accaacaggt gattggcgat ccgcgcaaag gcccaacgct cgccgcgggc
     2641 gatccggccg gcggcccaaa cgtcggagac aatcgcaacc gccccgggcg ccaattccac
     2701 cacgagcgat tgggcgaacc gcgctccggc gaatggaatg accgggtccg gaatccactc
     2761 cagcacgccg cccgtttcga cgaccacgcg gacatccgac cgcgcctcca cccccgcgca
     2821 tcgatagacc ttggtcgccg actgcgtggt cgccagcgcg tgcgcaccgg ctcggacggt
     2881 caaatcgtgt cgcaaccggt caccgcccac gatccctccc gacacgttga gcagcgtgac
     2941 ataggcgcaa ccatccaggt caaccggcgg cggtacgcgc aagggcgcgg tcgacacatt
     3001 gcggcgcaga accgtgcggc gccccacccg ctcaaacagc aactccatcg agccgtccgt
     3061 cgtccggaca cgatcgccat tcccgtcccg cgggtccgtg gactctccga tcacgccgac
     3121 gccgcccggg atcactccac ggatcgccat gggttatcga accgacaccg gagaccccac
     3181 tcggcgttga acccacgtct cgacgccgtc aagaccctgc ccggatttga ggttgctgaa
     3241 gaggtacggc cgatcgcccc gcatccgccc cgcatcgcga tccatcacgc cgaggtccgc
     3301 tccaacgtgc ggcgccagat cgattttgtt gatcaccagc aggtctgacc gcgtaatgcc
     3361 cggccccccc ttcctcggaa tcttgtcccc cgccgcaacg tcgatcacgt agatcgtcgc
     3421 gtccaccaac tcgggactga acgtcgccgc cagattgtcc ccgccgctct cgatgaacac
     3481 caggtcaaga tcgggaaatc gcgcgaccaa ctgctggagg gcttcttgat tgtgcgacgc
     3541 atcttcgcga atcgccgtat gcgggcaccc ccccgtttca accccgatga tccggtcggc
     3601 cgccaacacc ccgcggcgcg tgaggaactc ggcgtcctct cgcgtaaaga tgtcgttggt
     3661 gatgaccgcc acccggtggt catggaccag tcggcggcac aacgcttcga ccagcgcggt
     3721 tttcccggac ccgaccggac ctcccacccc gatgatcacc ggatagttca tggctctcct
     3781 cccatgttag gaacgaaaca atcgcgttgg ctgccgttcg tgccggatca tccgaatgtc
     3841 atgcatcgga gtccacgacc gcatcgaccc cacgtcgccg gactcggcct cccgcgccgc
     3901 cgactcgatc accggcagga gcgaggcgag gacgcgttgc cccccgcgct gaccgaccgg
     3961 catgagccga atcgccgcag acacccatcc caccgcggtc tggtagagcg cggcgccgat
     4021 cgcgtcttgt cgcgaccacc cgcaggcagc caggacgctt cccagcgcgg tcgccgcgtg
     4081 cccgggcgcg tccccgtggc ggatcgccgc ctcatactgc cgcaagccct ccgtggcggc
     4141 acacaacacc ccccactcca gatatcgccg ccccatcact cggcttccct cgcgcgcctc
     4201 gcgggcgaac ttaaacgcct ccaggttcca gtccacgtcg acaagggagg cgacgtcgcc
     4261 ttgttcgagc gcgcgatgcg cggcggcaac cgcgacggcg tcgcaggtgg cgacaccgtc
     4321 acgaaggagg tgaccgaggt acgccgccac gtcgtcctcg ctcctgaccc gcccatcggc
     4381 gacgg
//
LOCUS       DBNGTT010000253         4047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1077636, whole genome shotgun sequence.
ACCESSION   DBNGTT010000253 DBNGTT010000000
VERSION     DBNGTT010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1077636"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..444
                     /locus_tag="AC16BC_05650"
     CDS             235..444
                     /locus_tag="AC16BC_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241138.1"
                     /translation="MSHVASHAAFERVVIHTRPHVAAPAASACCQQAVVEALETAGSF
                     GFIATLTMLFIGFGMGLTTAWMLFW"
     gene            627..2804
                     /locus_tag="AC16BC_05655"
     CDS             627..2804
                     /locus_tag="AC16BC_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3241139.1"
                     /translation="MPGACLVPTRLTTWLAVALGLQLSVAACSGRPWNSPYPASDENK
                     NILYSSFDERPKHLDPARSYSSNENAFIAQIYEPPLQYHFLKRPYELIELTATEVPKP
                     AYFDAQHRRLPDTAPTEQIAYSAYDIRIQPGIRYQPHPAFATDAAGRFLYHHLTPSDL
                     ERVATLADFPQSGTRELIADDYVYEIKRLAHPKLHSPIFGLMSEYIVGLGEYGTTLEK
                     AAADHPTEFLDLTQYPLEGAVAVDRYTYRLTVKGKYPQMVYWLAMPFFAPIPPEVDRF
                     FSQPGMEQKNLTLDWYPVGTGPFMLTVNNPNRQMVMERNPNFHGERYPAEGEATDQAA
                     GYLTDAGKPLPFIDKAVYSLEKESIPYWNKFLQGYYDVSGISSETFDQAVQMTGHGEV
                     GLTDAMETKGIRLVTSVETSIFYLGFNMLDPVLGGYSDRSRKLRQAISIAYDDDEFIS
                     IFRNGRGSAAQGPIPPGIFGYVDGEHGINPYVFAWVDNQPRRKSIDDANRLLVEAGYP
                     NGRDSRTGKPLVLHFDVTAGGADDKARLDWVRKQFAKIDIELEIRDTDYNRFQDKMLN
                     GSAQIFQWGWNADYPDPENFLFLLYGPNAKVGKNGENAANYENPEFDRLFERMKNMDN
                     GPERQNIINQMVEIVRRDAPWAFGFHPKQFTLHHAWYKNAKPNLMANNTLKYKRIDPA
                     LRTQRRAEWNRPHLAPVAVALLLPVAVVLPAVIGYIRRNRSAG"
     gene            2801..3778
                     /locus_tag="AC16BC_05660"
     CDS             2801..3778
                     /locus_tag="AC16BC_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241140.1"
                     /translation="MIRYLVRRTLYAIPILIGVNLITFVLFFVVNPPDNMARIHLGIK
                     RVTPEAIAKWKQDHGYDKPVLWNAAASGAGKATDTIFFEKSLALFAFNFGPADDGRDI
                     GADIQQRMWPSLAIAVPMFMVGILINITFAMTLAFFRGTYLDLWGVITCVIMLSISAL
                     FYVIGGQYLIGKLLNLTPISGYDTGFSAAKFLILPVIVGIVSGVGASTRLYRTIFIEE
                     INKDYIRTARAKGLSEVRVLFKHGLKNALIPILTGVVVIIPSLFLGSLILESFFGVPG
                     LGSYTIDGIRSQDFAIVRAMVFLGSTLYIAGLILTDVSYTLVDPRVRLE"
     gene            3778..>4047
                     /locus_tag="AC16BC_05665"
     CDS             3778..>4047
                     /locus_tag="AC16BC_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241141.1"
                     /translation="MSVKPVVLWTDLLLFVLVGVAIAAALHARRREHLRAPWRYVRRQ
                     RLGMAALVILCAYAAIGLLDSIHFRLPLPPSNTPGSADRVDYSTEV"
BASE COUNT          770 a         1305 c         1192 g          780 t
ORIGIN      
        1 cctcctcctt gcggccttgc agccggagcc ttttgagcag cctcgggctg ttgcgtgctg
       61 gacattcgtc aggcgtaagg cccgtctgcc atgtttccgg caccgatggc ccggccggcc
      121 tcttggaatt cgccctctcg gtggcatacc cgttgaatat gtctcatggg gagagcacca
      181 ccgtcacacg ggcgggcctg gttcgcggcc cattgaacca caggaggaat tcacatgagt
      241 cacgttgcgt cccacgctgc attcgaacgc gttgtcatcc acacccggcc gcacgtcgcg
      301 gctccggctg cctcggcgtg ttgccagcaa gccgtggtcg aagcgctgga aaccgcgggg
      361 tccttcgggt ttatcgccac cctgaccatg ctgttcatcg ggttcgggat ggggctcacc
      421 acggcgtgga tgttgttctg gtaacggcgc cggctggcgt acccgcgcga cgcggcgcgt
      481 cgcgcgacgg gcgagcccgg ctcgttggat tgacgcgtcg agttgctgcg ccacccgcag
      541 ggatgcctgc ggcgacaatc ttcaggcgcg cccttggcgc gctgcgcgtt tgactttcca
      601 ccgagtgctt cactacgatg ccccctatgc cgggggcttg cctggtccca acccgcctga
      661 cgacatggct cgctgttgcg ctgggtctcc aactcagcgt tgccgcctgc tcaggcagac
      721 cgtggaactc gccctacccc gcttcggacg agaacaagaa cattctgtac tcgtcgtttg
      781 acgagcgacc caagcacctg gatcccgcgc gctcgtacag ctcaaacgag aacgctttca
      841 tcgcgcagat ctacgaaccg ccgctgcagt accactttct caagcggccg tacgaactga
      901 ttgagctgac cgcgaccgag gtgccaaaac ccgcgtactt tgacgcgcag caccggcggc
      961 tgcccgacac cgcgccgacc gaacagatcg cgtacagcgc gtacgacatt cgcatccaac
     1021 cagggatccg ctatcaaccg cacccggcgt tcgccacgga cgccgccggt cgtttcctct
     1081 atcaccacct cacgccgtcg gatctggagc gcgtcgccac gctcgcggac tttccgcaat
     1141 ccggcacgcg cgagttgatc gcggacgatt acgtgtacga gatcaaacgc ctcgcgcatc
     1201 ccaaactcca ttcccccatc ttcggcctga tgagcgagta catcgtggga ctcggcgagt
     1261 acgggaccac cctggaaaag gccgcggccg accacccgac cgagtttctc gatctgacgc
     1321 agtacccgct cgaaggcgcg gtcgccgtgg atcgatacac gtatcgcctc acggtcaagg
     1381 gcaaataccc gcagatggtc tactggctcg ccatgccctt cttcgcgccg atcccgcccg
     1441 aagtggaccg gtttttttcg caacccggca tggagcagaa aaacctcaca ttagattggt
     1501 atcccgtggg caccggcccg ttcatgctca ccgtgaacaa tcccaaccgc cagatggtca
     1561 tggagcgcaa tccgaacttc cacggcgagc gctaccccgc cgagggcgag gccaccgacc
     1621 aggccgcggg gtacctcacg gacgcgggca aaccgctccc gttcatcgac aaggccgtgt
     1681 acagcctgga gaaagagagc atcccgtact ggaacaagtt tctccaggga tactacgacg
     1741 tgagcgggat cagctccgag acgttcgatc aagccgtgca gatgacggga cacggcgagg
     1801 tggggctgac cgacgcgatg gaaaccaagg gcatccgcct ggtcacgtcg gtcgagacgt
     1861 cgatctttta tctgggcttt aacatgctcg atcccgtgct cggggggtac tcggatagaa
     1921 gccgcaaact tcggcaagcc atttcgatcg cgtacgacga cgacgaattc atttcgatct
     1981 ttcgcaacgg gcgcgggagc gcggcgcaag ggcccattcc tcccggcatt ttcgggtatg
     2041 tggacggcga acacgggatc aacccatacg tgttcgcctg ggtggacaat cagccgcgcc
     2101 gcaaatcgat cgacgacgcc aaccgcctat tggtcgaagc cgggtacccc aatggccgcg
     2161 acagccggac cggcaaaccc ctggtgctgc acttcgacgt gacggcgggg ggagcggatg
     2221 acaaggcacg actggattgg gtgcgcaaac aattcgcgaa gatcgacatt gaattggaaa
     2281 ttcgcgacac ggactacaac cggtttcaag acaaaatgct caacggctcc gcgcaaatct
     2341 tccaatgggg ctggaacgcg gattacccgg acccggaaaa ctttctgttt ctgttgtacg
     2401 gccccaacgc caaggtagga aagaacggcg agaacgcggc caactatgag aaccccgagt
     2461 tcgaccgatt gttcgaacgc atgaagaaca tggacaatgg cccggaacgc cagaacatca
     2521 tcaatcagat ggtggagatc gtccggcgcg acgcgccctg ggcgttcggg ttccacccca
     2581 aacagttcac cttgcaccac gcgtggtata aaaatgccaa gcccaatttg atggcgaaca
     2641 acaccctcaa gtacaaacgc atcgatcccg cgctgcgaac gcaacggcgc gcggaatgga
     2701 accggcccca cctggcgccg gtggccgtgg ccctgctgct gcccgtcgcc gtggtgctgc
     2761 ccgcggtgat cgggtacatc cgtaggaatc ggtcggccgg atgatccgct acttggtccg
     2821 ccgcaccttg tacgccattc cgatcctcat cggcgtgaac ctgatcacgt tcgtcctgtt
     2881 cttcgtggtc aatccccccg acaacatggc gcgcatccat ttgggaatca aacgggtcac
     2941 accggaggcc atcgccaaat ggaaacagga ccacgggtac gacaaacccg tgctctggaa
     3001 cgccgcggcc tccggcgcgg gcaaagccac ggacacgatt tttttcgaga agtccctggc
     3061 gttgttcgcg ttcaacttcg gacccgcgga cgacggacgc gatatcggcg ccgacatcca
     3121 gcagcggatg tggcccagcc tcgccatcgc ggtgccgatg ttcatggtcg ggatcttgat
     3181 caacatcacg ttcgcgatga cgctcgcgtt ttttcgcggc acgtaccttg atctctgggg
     3241 agtgatcact tgcgtcatca tgctgtccat ctcggcgttg ttctacgtga tcggcggaca
     3301 gtacctgatc ggcaaactgc tgaacctcac gcccatttcg ggctacgata ccggattttc
     3361 cgccgccaaa ttcctgatcc tcccggtcat cgtcggcatc gtgagcggcg tcggggccag
     3421 cacgcggctg tatcgcacga ttttcatcga agaaatcaac aaggactata ttcgcaccgc
     3481 gcgcgccaaa gggctgtcgg aagttcgcgt gctcttcaaa cacggcctga aaaacgctct
     3541 gatcccgatt ctgaccggcg tggtggtgat cattccgtcg ctgtttctgg ggagtttgat
     3601 cctggaatcg ttcttcggcg tgccaggcct cggcagctac acgatcgacg gaattcgatc
     3661 ccaggatttc gcgatcgtgc gcgccatggt gttcctgggc tccacgctgt atatcgcggg
     3721 gctgatcctc accgacgtgt cgtataccct ggttgatccg cgggtgcggc tggaatgatg
     3781 tcggtcaaac ccgtggtgtt gtggacggat ctgctcctgt tcgtcctggt cggggtggcg
     3841 atcgcggcgg cgctccatgc gcgacggcgc gaacacctgc gcgcgccgtg gcgctacgtg
     3901 cggcggcaac ggctgggcat ggccgcgttg gtcattctct gcgcgtacgc ggcgatcggc
     3961 ttactggact cgattcactt ccgtctgccg ttgcccccgt cgaatacccc gggttccgcc
     4021 gatcgcgtgg actactccac cgaagtg
//
LOCUS       DBNGTT010000254         2598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2355840, whole genome shotgun sequence.
ACCESSION   DBNGTT010000254 DBNGTT010000000
VERSION     DBNGTT010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2355840"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="AC16BC_05670"
     CDS             <1..577
                     /locus_tag="AC16BC_05670"
                     /inference="COORDINATES: protein motif:HMM:NF025109.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein DrrA1-3 family
                     domain-containing protein"
                     /protein_id="HIR3241142.1"
                     /translation="MAGLTDHRARLTREWPLGWKQRLALGCAVLHEPPMLFLDEPTSG
                     VDPLSRRAFWELINSLTDQGITVFVSTHYMEEAEYCRRLALMNRGRLIALDTPSNLRA
                     GMVQPIIEVHCDRSPQAVEALSGLPGVSEASMFGRTVHVVVDDAVVARAAITDRLAAR
                     GVTVRSMERIEPSLEDVFVALVRAEGGAVLG"
     gene            739..1854
                     /locus_tag="AC16BC_05675"
     CDS             739..1854
                     /locus_tag="AC16BC_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241143.1"
                     /translation="MNFRRLLAISRKELIQLRRDTRSLLLAFLLPVLLLVLFGYAITF
                     DVEHIATAVLDQDRSARSRELIEAFKSSGYFTVTEELERSSDIDPALKGWRAQVALVV
                     PPDFAAHLGAGRQAAFQVLVDGSDANTATIILAYAQAVVQTYSTAVTVQGRSVRPLIT
                     ADTRVWYNEELTSRNMIVPGLVAVIMMIIAAMLTSLTIAREWERGTMEQLAATPVTRV
                     EVVVGKLLPYLGIGLIDVVTSTALGVALFGVPFRGDPFLLMILAFFFLIGALGLGIFI
                     SAAARSQLLATQMAMMATFLPAFLLSGFMYAIDVMPKALQVLTYLVPARYFLVVTRGI
                     FLKGVHVEVLRAQGLLMIVFAGAGLLLAIRAFRKEIG"
     gene            1885..>2598
                     /locus_tag="AC16BC_05680"
     CDS             1885..>2598
                     /locus_tag="AC16BC_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241144.1"
                     /translation="MAWSDKFERIFELVRKELLQIMREPRVRRMIVVAPMIQLVVFGY
                     AVSTDIRHTATFVVDHDRTRDSRELVEAFVASGYFRVVGRSDRPDDLVSALDHGNAIV
                     GVQIPAGYAAALQGGEGATVQIVLDGTQSNTATVARGYAERIVQSVAARAGSAPLPAV
                     DLRERAWFNPDLASRNYNVPAVVGTIILLVCLLLTSLAVVREREIGTLEQLMVSPLTP
                     GELIAGKTIPFALIGIMDLA"
BASE COUNT          391 a          776 c          923 g          508 t
ORIGIN      
        1 gatggcgggg ctcacggatc atcgcgcgcg attgacccgc gaatggccgt tgggttggaa
       61 gcagcggttg gcgttgggct gcgcggtcct gcacgaaccg cccatgctgt ttctggatga
      121 gccgacctcc ggggtggacc cgctgtcgcg gcgcgcgttc tgggaattga tcaacagcct
      181 gaccgaccag gggatcacgg tgttcgtgag cacgcactac atggaggaag cggagtattg
      241 ccgccgcctg gcgctgatga accgcgggcg gctcatcgcg ctggacacgc cgtcgaacct
      301 gcgcgcgggg atggttcagc ctattatcga agtgcactgc gaccgcagcc cccaggcggt
      361 cgaagccctc agtggactgc cgggagtttc ggaagcctcg atgttcggcc ggacggttca
      421 cgtcgtggtg gatgacgcag tcgtggcgcg ggcagccatc acggatcgac tggcggcgcg
      481 cggcgtcacg gtgcggagca tggagcgtat tgaaccatca ttggaagacg tctttgtggc
      541 gctggtacgt gctgaaggcg gcgcagtgct tgggtaaagc ctgtcgatga acggccatga
      601 acggcaaatc cccccttacc cccctttgcc aaagggggga tggggggatt taaggcgcgt
      661 gaacggctcg tccgtggagc gtcaccgatg tcctgttcct ctattaaccg gtggtggaat
      721 catgcgaggt aaggtttttt gaactttcgc cgattgctcg ccatctcacg caaggagttg
      781 atccagctcc gccgcgacac caggagcctg ttgctcgcgt ttctcttgcc cgtgttgctc
      841 ctggtcttgt tcgggtacgc gatcacgttc gacgtggagc acattgccac cgcggtgctg
      901 gatcaggatc ggtcggcgcg gagccgggag ttgatcgagg cgttcaagtc gtcgggctat
      961 ttcaccgtca ccgaggagtt ggagcgatcg tccgacatcg atccggcgtt gaaggggtgg
     1021 cgggcgcagg tcgcgctcgt cgttccgccg gacttcgcgg cgcacctggg cgcggggcgc
     1081 caggccgcgt tccaggttct ggtggacggc agcgacgcga acacggccac catcatcctg
     1141 gcctacgcgc aggccgtggt gcagacgtac tccaccgcgg tcacggtgca gggccgatcg
     1201 gtccggccgt tgatcacggc cgacacgcgc gtgtggtaca acgaggagct gaccagccgg
     1261 aacatgatcg tgccggggct ggtggcggtg atcatgatga tcatcgcggc catgctcacc
     1321 tcgctcacca tcgcccgcga atgggagcgc gggaccatgg agcaactcgc ggccacgccg
     1381 gtcacgcggg tggaggtggt ggtgggcaag ctgctgccgt acctcggcat cgggttgatc
     1441 gacgtggtga ccagcacggc gctgggcgtg gcattgttcg gggtgccgtt tcgaggcgac
     1501 ccgtttctgc tgatgatcct cgcgttcttt tttctgatcg gcgcgctcgg tctggggatc
     1561 ttcatttccg cggcggcgcg ctcccagttg ctcgccaccc agatggccat gatggcgacg
     1621 tttctgccgg cgtttctgct ctccggcttc atgtacgcga tcgacgtgat gcccaaggcg
     1681 ctgcaagtgc tcacgtacct ggttcccgcc aggtatttct tggtggttac ccgcggcatc
     1741 ttcctgaaag gcgtgcacgt ggaggtgctc cgggcgcagg gcttgctgat gatcgtgttc
     1801 gccggcgcgg gcctgctgct ggcgatccgc gcgttccgga aggagatcgg ataaggtcgc
     1861 cggattcggg aggacggacg ctccatggca tggtccgata agttcgaacg catctttgaa
     1921 ctcgtcagga aggaactgct ccagatcatg cgcgagccgc gcgtgcggcg catgatcgtg
     1981 gtggcgccca tgatccagct cgtggtgttc ggctacgcgg tctccacgga cattcgtcat
     2041 accgcgacgt tcgtggtgga ccacgaccgg acgcgggact cgcgggagct ggtcgaggcg
     2101 ttcgtggcct ccggatactt tcgcgtggtc ggccggtccg atcgtccgga cgatctggtg
     2161 tcggcgttgg atcacgggaa cgcgatcgtg ggcgtgcaga ttccggccgg ctacgcggcc
     2221 gcgctccagg gcggggaggg cgcgaccgtg cagatcgtgc tggacggcac gcagtccaac
     2281 accgccacgg tggcgcgagg ctacgcggag cgcatcgtgc agagcgtggc cgcgcgcgcc
     2341 gggtccgcgc cgttgcccgc ggtggacctg cgggagcgcg cctggttcaa cccggacctg
     2401 gccagccgca attacaacgt gcccgcggtg gtgggcacga tcatcctgct cgtctgcctg
     2461 ctgctcacgt cgctggccgt ggtgcgcgaa cgggagatcg ggacgctcga acagctcatg
     2521 gtgagtccgc tcaccccggg ggagttgatc gcgggcaaga ccattccgtt cgcgctgatc
     2581 ggcatcatgg acctggcg
//
LOCUS       DBNGTT010000255         7389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_567709, whole genome shotgun sequence.
ACCESSION   DBNGTT010000255 DBNGTT010000000
VERSION     DBNGTT010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_567709"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..640
                     /locus_tag="AC16BC_05685"
     CDS             128..640
                     /locus_tag="AC16BC_05685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3241145.1"
                     /translation="MADRSDSASWAPSLPTETPTREVPIEEPQPRPAYGLAFSLFVAT
                     LGTTVLAGAFMDGADPFARPSELVRGIPFSLTLMAILLTHEMGHYITSRHYGVHASLP
                     YFIPAPPLLFMIGTFGAFINMRSPIMRRGPLLEIGAMGPIAGFVVAVVAVIAGLLQSS
                     VQPANSLDGM"
     gene            677..1069
                     /locus_tag="AC16BC_05690"
     CDS             677..1069
                     /locus_tag="AC16BC_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3241146.1"
                     /translation="MLINAPAEGYDLVLHPVAFAGWIGLFVTALNLLPIGQLDGGHIT
                     YALFGKRHKVISIVTVLSLGVLGSLSVFGVNAWPGWIVWGILGAIIGLRHPPVIDQDV
                     PLTRRQRVIAWASVAIFVLTFTPVPFVV"
     gene            complement(1066..2346)
                     /locus_tag="AC16BC_05695"
     CDS             complement(1066..2346)
                     /locus_tag="AC16BC_05695"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3241147.1"
                     /translation="MTDAPASSRERWAWCGYDFANSAFTTIIVTVAYSVYFTRVVAPE
                     DGEAWWGRGYAASMLLAGLLSPILGAVADATASKRRWLIVMTVVCVAPTALLGWVGPG
                     DLAWGLGLFIVANVGYNAALHLYDAFLKELTPADTLGRLSGWGWGFGYLGGLVSLALA
                     YPFLAGGMEGEAAAHYRLAFPVTAAFFALAALPTFVWLTERAAPVPNAGSPWLAGIRR
                     VVETLRHLGRHRQLVRYFVAYFVFNDAVNTVFVFAAIFATQVLAFTAQDLVIFFLIMQ
                     LSSAAGAGAFGWVTDRLGGVRAISSTLVAMIGITVWAARVQTTSEFYVIGLFTGALLG
                     ANQAASRTLLAHFAPPGRGAEFFGLFSLTSKFAATLGPLLYGEIARATGSHRTAVLSI
                     GALFLAGLLLLQRVNEAAGRHEATAGYDAGPHPD"
     gene            complement(2339..3136)
                     /locus_tag="AC16BC_05700"
     CDS             complement(2339..3136)
                     /locus_tag="AC16BC_05700"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3241148.1"
                     /translation="MTQRTWLPLAVEAARAAGDIIRRGMSDTLDIRYKGAINLVTQID
                     VAAERAVVERLRTKAPDHNILAEEGTTQIGDSPYRWLIDPLDGTTNYAHRFPFFCVSI
                     GLQREQDMVLGVVFDPLRDELFTAVRGGGAALNGRPLSVSATAVLHHSLVVTGFAYDP
                     ARDTNVNFRHFEAMSRVVRGVRRTGSAALDLCYVAAGRSDGFWELALSPWDTAAGQVI
                     VDEAGGRVSDFGGRAFSPYIPEVVASNGKIHDAMLAVLNQRAAPSRD"
     gene            3205..4575
                     /locus_tag="AC16BC_05705"
     CDS             3205..4575
                     /locus_tag="AC16BC_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3241149.1"
                     /translation="MSAKRTFARRTRRGRGRIRRAMLALALPVVASSLLERAVGIIDV
                     FLVGGLGASAIAAVGASQLLVFMVSSMVAGLPAGTVVVVAQLWGAGRRPEAAAGARRV
                     AGIALATGVAVGLCGMLWAAHAVMLLGASPEVVGLAQPYLRIVFAAFPFVFLVTVFSG
                     ILQGTGDTRSPLYVTTLMNIIHVAVAYPTIYGAWGVPRWELEGAALAVAVSNAVGAGC
                     LAVIAYRRGLLARGRSDHFVRAVARVGAPVSVDRTLQQAAQLVFAKVVLLYGTTAYAA
                     HQVGLAIEALSYLPGAGFALAAETTFGRSLGARTVARARMQSLEASRMALVVMAAMGV
                     LFYVSPYVLLRLFTDDRTVIDLGTRFLHIVALLQIPLALTMVVSGTLKGAGDTRFLLF
                     STLAGAWGVRVPLALLFAYGLHLSLAAVWSVMLADWMVRMVVVLARYRSGAWRSHRVL
                     WGAEAR"
     gene            4631..4930
                     /locus_tag="AC16BC_05710"
     CDS             4631..4930
                     /locus_tag="AC16BC_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241150.1"
                     /translation="MRRMTVVAAVAVIVAGAMALAVPRYAAACKSAGAFKHVGVVTAV
                     DLTTLTVTITDAESGKPVTFSATAQQLKDVKPGDEVMIGFLEKDGKLTAVQIQSS"
     gene            complement(4948..5952)
                     /gene="ygfZ"
                     /locus_tag="AC16BC_05715"
     CDS             complement(4948..5952)
                     /gene="ygfZ"
                     /locus_tag="AC16BC_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR3241151.1"
                     /translation="MLQTTDADTGYRVLRTGAGILDCSSRVRLRVTGSMRAKFLQGIL
                     TNDTNLLGPGRGLYAAVLTGKGKMQSDLTVYSVSDWYWIDAEPEVADSLPALIGRYTI
                     GTDAVVTNLAATHRTLAVYGPTATRVITAGLGAPAPAGNLAVMEMDWAGHRLVVGESG
                     LPGVPGYKILLPFDAVEKAVDALTAQGAGRVDGAALETIRIESGIPRFGPDMSDENFP
                     PEARIESRAISYTKGCYMGQETIARINTYGHVNRLLVGLLPDTDQPLTAGTRIVSTGS
                     DAKDVGYVTSAAFSPTVGRVIALGYVHRSLEAPGTVVLLGTEPPVRAVVAALPFAATT
                     "
     gene            6084..6527
                     /locus_tag="AC16BC_05720"
     CDS             6084..6527
                     /locus_tag="AC16BC_05720"
                     /inference="COORDINATES: protein motif:HMM:NF048679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI/CycH"
                     /protein_id="HIR3241152.1"
                     /translation="MTLRSVVVAVVFTTTVVLIGAGCSKKNEPPKPATISGTVTVAPE
                     LAKDITPQHILFIVVRPADAPPFGPPVAAHRMTNLTFPVQYTVTQKDVFFEGVTLEGK
                     VNVYAKLDKDGNAGPPEPGDLEGEFKGNPIMVGATGVDIVVDKIH"
     gene            6919..>7389
                     /locus_tag="AC16BC_05725"
     CDS             6919..>7389
                     /locus_tag="AC16BC_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241153.1"
                     /translation="MKRRLVFGTVLAALVGVITPSWALDVKWGGDYRLRGFYADNLSD
                     ADDNTQDSIAYMNSRFLLTTAVSEDGVSGVITLIATDTNRNGNRLLGGDPANGGAYGP
                     SGSTVRMLEAYLTADFKSWTAKGGRAVFKLGNGIILDDSVDGLWANFPIGGMDVT"
BASE COUNT         1210 a         2351 c         2493 g         1335 t
ORIGIN      
        1 ggccgactcg tcggcgccga cgagtacgag ctgtatttgc tgacgcccgg cggctcattc
       61 aaccttccgc tggaacatat tacgtcggtg cgccaggccg cgtagccacc tcccggaaac
      121 gagaaccatg gccgaccggt cagactccgc atcctgggcg ccgtcgttgc cgacggagac
      181 cccgacgcgc gaggttccga tcgaggaacc gcagccgcga ccggcttatg ggttggcgtt
      241 ctccctcttt gtggccacgc tcgggaccac ggtgctggcg ggcgcgttca tggatggggc
      301 ggatcccttt gcccggccca gcgaactcgt gcggggcatc cccttttcgc tgaccctgat
      361 ggcgattctc ctgacgcatg agatgggcca ctacatcacg tcgcgtcatt acggcgtcca
      421 cgcgagcctc ccgtacttca tccccgctcc gccgctcctg ttcatgatcg gaacgttcgg
      481 cgcgttcatc aacatgcgct ccccgatcat gcgccgcggc ccgctcttgg agatcggcgc
      541 catggggccg attgccgggt tcgtggtcgc ggtggtcgcc gtcattgccg ggctcctgca
      601 gtcgtcggtc caacccgcga actcgcttga cggcatgtag ctgggatctc cactactcct
      661 gcatgcgctt ggcgcagtgt tgatcaacgc gccggccgag gggtacgacc tcgtgctcca
      721 tcccgtggcc tttgccgggt ggatcgggtt gttcgtgacc gcgttgaacc ttttgccgat
      781 cggtcaactc gacggcgggc acatcaccta tgcgttgttc gggaagcgac acaaagtgat
      841 ttcgatcgtg accgtgctgt cactaggcgt cctgggttcg ctcagcgtgt tcggggtgaa
      901 cgcgtggcct ggctggatcg tgtgggggat tctcggcgcg atcatcgggt tgcgccatcc
      961 cccggtcatt gatcaggacg tgccgctcac gcgtcggcaa cgcgtgatcg cgtgggccag
     1021 cgtcgcaatt tttgtcttga cgttcacgcc cgttccgttc gtggtctaat ccgggtgggg
     1081 gccggcgtca tatccggcag tcgcctcgtg ccgcccggcc gcttcgttta cacgctgcaa
     1141 caacagcagt cccgccaaga acaacgcgcc gatcgacaag accgcggtcc ggtggctccc
     1201 ggtcgcccgg gcgatttcgc cgtacagcaa cgggccgagc gtggcggcga atttgctggt
     1261 cagcgaaaac aacccgaaga attccgcgcc gcgtccgggc ggagcgaagt gcgccaacag
     1321 ggtccggctg gccgcctggt tggcgcccaa caaggcgccg gtgaacaacc cgatgacgta
     1381 aaactccgag gtggtctgaa cccgcgcggc ccagaccgtg atgccgatca tcgcgacaag
     1441 cgtgctggag atggcgcgca cgccgcccag tcggtccgtc acccatccga acgcgccggc
     1501 gccggccgcg gacgagagtt gcatgatgag aaagaagatc accaggtctt gcgcggtgaa
     1561 cgccaggacc tgggtcgcga agatggcggc gaagacgaac accgtgttga ccgcgtcatt
     1621 gaagacgaaa tacgccacga aataccggac cagttggcga tggcgtccaa gatgtcggag
     1681 cgtctcgaca acgcgacgaa tgcctgcgag ccacggggac cccgcattgg gaacgggcgc
     1741 ggcgcgctct gtgagccaga cgaacgtggg cagcgcggcc aaggcgaaga acgccgcggt
     1801 caccgggaac gccaatcggt agtgcgcggc ggcctcgccc tccatgccgc ccgcgaggaa
     1861 cgggtaagcc agggcgagcg acaccaaacc gccgaggtat ccgaaccccc agccccatcc
     1921 cgacaggcgt ccgagcgtgt cagcgggcgt gagttctttc agaaaggcgt cgtacaggtg
     1981 gagcgcggcg ttatagccga cgttggcgac gatgaagagc ccgagtcccc aggccagatc
     2041 gccaggtccc acccaaccca acagagcggt cggggcgacg cacacgaccg tcatgacgat
     2101 caaccagcga cgtttggacg cggtggcgtc ggccacggcg ccgaggatcg gcgacaacag
     2161 tcccgcgagg agcatggacg ccgcgtatcc gcgtccccac caggcctcgc cgtcttcggg
     2221 cgcgaccacg cgcgtgaagt acacgctgta cgccaccgtg acgatgatgg tggtgaacgc
     2281 ggagttggcg aagtcgtagc cgcaccacgc ccaacgctcc cgcgaggacg ccggtgcgtc
     2341 agtcacgcga cggggcggcg cgttggttga ggaccgccag catggcgtcg tggatcttgc
     2401 cgttgctcgc gacgacttcg gggatatagg gggaaaaagc gcgaccgccg aagtcgctga
     2461 cgcgtccgcc cgcttcgtcc acgatgacct gtcccgcggc ggtatcccac ggcgagaggg
     2521 ccagctccca aaacccgtcg gaccggcccg cggccacgta acacaggtcc aaggcggcgg
     2581 agcccgtccg ccgaactccg cggaccacgc ggctcatggc ttcgaagtgc cggaagttga
     2641 cgttggtgtc gcgcgcgggg tcgtaggcaa atcccgtgac cacgaggctg tgatggagca
     2701 cggcggtcgc ggaaaccgac agcggccgtc cgttgagcgc ggcaccgccg ccgcgaaccg
     2761 ccgtgaacag ttcgtcgcgc aacgggtcaa acaccacccc gagcaccatg tcctgctcgc
     2821 gctgcaatcc gatggacacg cagaagaacg gaaaacgatg cgcgtagttg gtcgtcccgt
     2881 ccaacgggtc gatcagccag cggtaggggg agtcgccgat ctgcgtggtg ccttcctcgg
     2941 ccagaatgtt gtggtcgggc gctttggttc gcagccgctc gaccaccgcg cgctcggcgg
     3001 ccacgtcgat ctgcgtcacc agattgatcg cgcccttgta ccgaatgtcg agcgtatcgg
     3061 acatgccgcg acggatgatg tcgcccgcgg cgcgcgccgc ctcgaccgcc aacgggagcc
     3121 acgttcgctg tgtcatgtcc gggttgcgca tggcgggcat cataccatac ggccgcgcgg
     3181 ggccgacgcg agcgcgcggt gcgggtgagc gcaaagcgta cgttcgctcg gcggacgcgg
     3241 cgcggacgcg gacgaatccg ccgcgccatg ctcgcgttgg cgctgcccgt ggtggcgtcc
     3301 agtttattgg agcgcgccgt ggggatcatc gacgtgttcc tggtgggcgg tctcggcgcg
     3361 tccgcgatcg ccgcggtcgg cgcgagtcag ctgttggtct tcatggtgtc gagcatggtg
     3421 gccggccttc ccgcgggcac cgtggtggtg gtggcgcagc tgtggggcgc cggacgacgc
     3481 cctgaggccg cggccggcgc acgccgcgtc gcgggtattg ccctggccac cggcgtggcc
     3541 gtcggcctgt gcgggatgtt gtgggcggcc catgccgtca tgctcctcgg ggcgtcgccc
     3601 gaggtcgtgg ggctcgcgca gccgtatctg cgcatcgtgt tcgcggcgtt tcccttcgtg
     3661 tttctcgtga ccgtgttctc cggcattctt caaggcacgg gtgatacccg gagcccgctc
     3721 tacgtaacga cgttgatgaa catcattcac gtggcggttg cctaccccac gatctacggc
     3781 gcgtggggtg tccctcgttg ggaactcgaa ggcgcggccc ttgccgtggc ggtctccaac
     3841 gcggtcggag cgggatgcct ggcggtgatc gcgtatcgcc gcgggctgct cgcgcggggc
     3901 cgcagcgacc attttgtccg cgcggtggcg cgggtgggcg cacccgtgtc cgtcgatcgc
     3961 acgctccagc aggccgcgca actggtcttc gcgaaggtcg tgttgctcta cggcaccacg
     4021 gcctacgcgg cgcaccaggt ggggcttgcc atcgaggcgc tctcgtatct tcccggcgcg
     4081 ggattcgcgc tcgcggcgga gaccacattc gggcgcagtc tcggcgccag gaccgtggcg
     4141 cgcgctcgta tgcagagcct ggaagccagt cgcatggctc tggtcgtgat ggccgccatg
     4201 ggcgtgttgt tttatgtctc gccgtacgtg ttgttgcggc tttttaccga cgatcgaacg
     4261 gtgattgatc tgggcacccg gttcctgcac atcgtggcgc tcctccagat tcccttggcc
     4321 ctgaccatgg tggtgtcggg tacgctgaag ggcgcgggtg acacgcgatt cctgctgttc
     4381 agcacgctcg cgggagcgtg gggggtgcgt gtgccgttgg cgttgttgtt cgcgtacggg
     4441 ttgcacctgt cgttggccgc ggtgtggagc gtgatgctgg ccgattggat ggtgcggatg
     4501 gtggtggtcc tggcgcggta tcgctcaggc gcttggcgat ctcatcgcgt cctgtggggg
     4561 gctgaggcgc gttgacagcg aatccggcgc ggcgatacga tcgcggtacg actcagcaag
     4621 gaggtgggtg atgcgacgga tgacagtggt ggcggcggtc gcggtaatcg ttgcgggtgc
     4681 catggcgctc gcggtcccgc ggtacgcggc ggcctgtaag agcgcggggg ctttcaaaca
     4741 cgtaggcgtc gtcaccgcgg ttgacctgac gacgctgacg gtcacgatta ccgacgcgga
     4801 atccggcaag ccggtcacgt tctcggccac ggcccaacaa ctcaaagacg tgaaacctgg
     4861 cgatgaggtg atgatcgggt tcctggagaa agacgggaaa ctgaccgccg tgcagatcca
     4921 gtcgtcgtag tggcgggacg cccgatctca ggtcgtcgcg gcgaacggga gtgcagccac
     4981 gacggcgcga accgggggct cggttccgag caggaccact gttcccggcg cttcgagcga
     5041 gcgatgcacg tatccgagcg cgatcactcg accgacggtt ggcgagaacg ccgccgacgt
     5101 gacgtacccg acatccttgg catctgatcc cgtcgacacg atccgggtcc cggcagtcag
     5161 cggctggtcg gtgtccggca gcagcccgac gagcaatcga ttgacgtgtc cgtaggtatt
     5221 gatgcgggca atggtttcct gacccatgta gcagcctttg gtgtagctga tggcccggga
     5281 ctcgatgcgc gcctccggcg ggaagttctc gtcgctcatg tcggggccga accgagggat
     5341 gccgctctcg atccggatgg tctccaacgc ggcgccgtcc acgcgtcccg cgccttgggc
     5401 ggtcagcgcg tcgacggcct tctccacggc atcaaacggc agcagaattt tgtagcccgg
     5461 tacgccggga aggccggact cgccgacaac cagccgatgg ccggcccaat ccatttccat
     5521 gacggcaaga ttcccggccg gtgccggcgc gcccaatccg gcggtgatca cgcgtgtcgc
     5581 tgttggcccg tacaccgcga gcgtacggtg cgttgcggcg aggttggtga ccaccgcgtc
     5641 ggtcccgatg gtgtatcggc cgatcagcgc ggggagcgag tcggcgactt cgggctcggc
     5701 gtcgatccag taccaatccg acacggaata gacggtcaga tccgactgca ttttcccctt
     5761 gcccgtgagg acggcggcgt agagccctcg ccccggaccg agcaggttcg tgtcgttcgt
     5821 caggatgccc tgaagaaact tggcgcgcat ggagccggtc acccgaagcc gcactcggct
     5881 cgagcaatcc aggatccccg cgccggtccg caacacccga taccctgtgt ccgcgtcggt
     5941 cgtttgcaac accaacagcc tccttcgtca ggcggggatc ttaccacggc ggattgccgt
     6001 cgaacaacgc acggcccggc gtcgttgaca ggacgcagtg ccttgcggga taatgccgcc
     6061 cgctcagcct gggaggggat acgatgacgc ttcggagcgt ggtagtcgcg gttgttttca
     6121 ccaccaccgt ggttctgatc ggggcgggat gctccaagaa aaacgaaccg cccaaacccg
     6181 cgacgatctc cggtacggtc acggtcgctc cggagttggc caaggacatc acgccgcaac
     6241 acatcctgtt tatcgtggtt cgtccggccg atgcgccgcc attcggtccg ccggttgccg
     6301 cccaccgcat gacgaacctg acgtttcccg tccaatacac ggtgacgcaa aaggacgtgt
     6361 ttttcgaagg cgtcacgctc gaagggaagg tgaacgtgta cgcgaagctc gacaaggacg
     6421 gcaacgcggg cccgcctgag cccggagacc tcgaagggga attcaagggc aacccgatca
     6481 tggttggggc gaccggggtg gacatcgttg tcgacaaaat ccactgagtt tcccgcgccg
     6541 tcgtccatgc tctccagccg cgcaggcgac cgtccccgta cctttatctg cgactactgc
     6601 ggcaatgcca tgttggacgt gcactgtaag ctcgtctgcc gccgctgcgg ctatatgcgc
     6661 gactgctccg acccgtaacg gtccggctgc cctctcctga tcgtcgcccc ccgcctcgtt
     6721 ccgttctact gctggacaga gccttttgcc ggtaaccgct tgacgtcttt gtaagccggc
     6781 atccgcatcc tttacagatt tttgatcacc ggctaccttt ttccgcgcca tggttggcac
     6841 gtgggaagaa cggccgccga gtggtccctg agtgatgcgg gtccggtcat cacacctctt
     6901 taggcaagga ggaacgcgat gaagcgacgt ctggtgttcg gaacggtcct tgcggccttg
     6961 gtcggcgtca tcacgccgtc ctgggcgctg gatgtcaaat ggggcggcga ttaccggttg
     7021 cgcggattct acgcggacaa cctctctgat gcagacgaca acacgcaaga cagcatcgcc
     7081 tatatgaact cccgctttct gctcactacg gcggtaagcg aggacggggt cagtggggtg
     7141 atcacgctga ttgcgactga tacaaacaga aacggcaata gactgctcgg tggcgacccc
     7201 gccaacggtg gagcctacgg tccttcgggg tcaaccgtgc ggatgctcga agcgtatctg
     7261 accgcggatt ttaagagctg gaccgcgaag ggcggacggg ctgtgttcaa actcgggaac
     7321 ggcatcattc tcgacgactc cgtggacggt ctgtgggcga acttcccgat cggcggcatg
     7381 gacgtcacg
//
LOCUS       DBNGTT010000256         2525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2740157, whole genome shotgun sequence.
ACCESSION   DBNGTT010000256 DBNGTT010000000
VERSION     DBNGTT010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2740157"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16BC_05730"
     CDS             complement(<1..473)
                     /locus_tag="AC16BC_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241154.1"
                     /translation="MRGRHAGWTAICAAVIGFAIGNGEPTIAQAGEHYPIISYDAFDY
                     GFRGADRVDPGLVNLQVVNTGKQPHQMQLVKLETGHTAADFAAAAKANPRRLPNWSRL
                     VGGPNGVVAGERTSTLQFLNPGDYVLVCWMPDANGTPHFTLGMTQSITVSEGAVKP"
     gene            complement(657..1427)
                     /locus_tag="AC16BC_05735"
     CDS             complement(657..1427)
                     /locus_tag="AC16BC_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3241155.1"
                     /translation="MASRPYIGTSGWHYPHWRGPVYPEGLPPAEWLAFYARRFKTVEI
                     NSSFYRLPDPDTIEAWRNAVPPGFRFAVKASRLITHMKKLTDPAATVPRLLDRVKGLG
                     PALGPILFQLPPRWHCAPERLAAFLAALPVGHRYAFELRDPSWHTPAVYRLLERAGAA
                     VCVYDLAGVVSPVIATAAFAYVRLHGPDGPYQGSYSRSALRTWARRIRQWRSLEAVFV
                     YFDNDEAGYAVGNASTLREIIQRGEDGRNGGGRGRPHP"
     gene            1507..2406
                     /locus_tag="AC16BC_05740"
     CDS             1507..2406
                     /locus_tag="AC16BC_05740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR3241156.1"
                     /translation="MRYSGAVATKKQLLGRVAAEIVGNGRLMTDRDCVEFLQWALPTL
                     RFRWPGFRKVRRIVCKRLDQRFRELDVSGPVAYRTYLEHHPEEWTELERFCAIPISRF
                     YRDRGVFETITRDVFPTLARSAVARGESTLRCWSIGCAAGEEPYTLAIIWRLGITERF
                     PCRLRIVATDVDGDLLARAGRGCYSPSSVKDVPAAWRTEAFTRSNGLYCVREEFRHAV
                     VFLRQDLRTAAPDGHFQLIVCRNLAFTYFEDRLQRDVLGTIRTHLAPDGVLVIGATES
                     LPESVGGFLPWPGAQGIYRRTDD"
BASE COUNT          435 a          858 c          778 g          454 t
ORIGIN      
        1 ggcttcaccg cgccctccga caccgtgatc gactgggtca tgcccaacgt gaaatgcggc
       61 gtgccgttcg catccggcat ccaacagacc aagacgtagt cgccagggtt caggaactgc
      121 agggtgctcg tgcgctctcc ggccacgact ccattggggc cgccgaccag ccggctccaa
      181 ttcggcagcc gccgcggatt ggctttggcc gcggccgcga agtccgccgc ggtgtggccg
      241 gtctccaact tcacgagctg catctggtgg ggttgcttgc cggtgttgac cacttgaaga
      301 ttgaccaggc cggggtccac gcggtccgct ccccggaacc catagtcgaa tgcgtcgtag
      361 gaaatgatcg gataatgctc ccccgcttgg gcaatcgtcg gctccccgtt cccgatggcg
      421 aacccgataa ccgccgcaca gatcgccgtc cagcccgcat gtctccctct catgcctcgc
      481 tcctttcgca caattttccg gatcaatcga gaacctgctc ccacgcacgg ctcgccggct
      541 aggcgagcgg cgcgacgctc ctcgcggaca acacgttaca acgaagcagt cggcactagt
      601 atccgtgatg tgctctcatc cgggatatac cccgaagggg ctaccgccgt tggggattac
      661 gggtgaggac gaccccgccc gccgccgttc ctcccgtcct cgccgcgttg aatgatctct
      721 cgcagcgtcg atgcgttgcc aactgcgtag ccagcttcgt cgttatcgaa atacacgaac
      781 acggcttcca gcgatctcca ttgtcggatt cggcgcgccc acgtgcggag cgcagaccgg
      841 ctgtacgagc cttgatacgg accatcaggg ccgtgtaggc gcacgtaggc aaacgccgcg
      901 gtcgcgatca cgggcgacac cacacccgcg aggtcataca cgcagaccgc cgcaccagcg
      961 cgttccagca accgatacac cgccggcgtg tgccaactcg ggtcgcgcag ctcgaacgca
     1021 taccgatggc ccacgggcag tgccgcgagg aacgccgcca gccgttccgg ggcgcagtgc
     1081 cagcgcggtg ggagctggaa caggatcggg cccaacgccg gcccgaggcc ctttacgcga
     1141 tcgaggagcc tcggcaccgt ggcggcggga tccgtgagct tcttcatgtg ggtgataaga
     1201 cggctcgcct tcacggcaaa acgaaatccg ggcggcaccg cgttgcgcca tgcctcaatc
     1261 gtgtccgggt cgggcagccg gtaaaagctg ctgttgatct ccacggtctt aaatcgccgc
     1321 gcgtagaagg ccagccactc cgcaggcggc agcccctccg gatacaccgg accgcgccag
     1381 tgcggatagt gccaaccgga ggttccgata tacggccgac tcgccatggt ccctcctgct
     1441 ctacgacggc tcgactgcac cgagcgatcc tcgcgcagac acgcgaggct gttgtcggga
     1501 gatcgaatgc ggtattctgg cgccgtcgcg accaagaagc aattgctcgg acgggtcgcg
     1561 gcggagatcg tcggcaatgg gcgactgatg actgatcgcg actgcgtgga attcctgcaa
     1621 tgggcgctcc caacgctgcg gttccggtgg cccggcttcc ggaaggtccg ccggatcgta
     1681 tgcaagcgcc tcgatcaacg gtttcgagaa ctcgacgtct cggggcccgt tgcatatcgg
     1741 acgtacttgg agcaccaccc ggaagaatgg accgaactgg agcgtttctg cgcgatcccg
     1801 atctcccgtt tttaccggga ccgaggagtc ttcgaaacga tcacgcggga cgtgttccct
     1861 acgctggcgc ggtccgcggt cgcccgaggc gaatcgacat tgcgctgttg gagcatcgga
     1921 tgcgccgcgg gcgaagagcc ctacaccctc gcgatcattt ggaggctcgg catcacggag
     1981 cggttcccct gccgccttcg gatcgtcgcg accgacgtcg acggcgacct gctcgccagg
     2041 gccggccgcg ggtgttattc cccgagcagc gtcaaagatg tgcccgccgc ctggcgcacc
     2101 gaagccttca cccggtcgaa tggactgtac tgcgtgcgcg aggagtttcg acacgccgtg
     2161 gtgttcctgc gccaggacct tcgcaccgcg gccccagacg gacattttca gctcatcgtc
     2221 tgtcggaatc tggcgttcac ctactttgag gaccgcctcc agcgcgacgt cctgggaacc
     2281 atccgcactc acctggcccc ggacggcgtg ctggttatcg gagccaccga gtcccttccc
     2341 gagtcggtcg gaggctttct gccatggccc ggagcgcagg gcatttatcg acgaaccgat
     2401 gactgacgcc gctttgagcc gtcccgttgc ccttgctata atctgcccgt gtcggatctt
     2461 gcggtcccaa ctctcgcgct gatcgacgaa ctcacccgcc tgttcgggcc caaccaggtc
     2521 ctggg
//
LOCUS       DBNGTT010000257         8334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2995312, whole genome shotgun sequence.
ACCESSION   DBNGTT010000257 DBNGTT010000000
VERSION     DBNGTT010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2995312"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1335)
                     /locus_tag="AC16BC_05745"
     CDS             complement(61..1335)
                     /locus_tag="AC16BC_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3241157.1"
                     /translation="MADGTETEVKTNPQGDVITATEAPKKEKENKAAKQRVVTPEHLF
                     FEAPRTKTFITGSEAAKEAIRRANVDIAISYPITPQSETMQQVGYLYAEGYLKDYYRG
                     EEEYGVMSAIAGASRSGVRALTATAGPGLMRGLEVITSWPGGRMPLLLLVMCRVINAP
                     LAIQPDNIELSYLLNCGILVFHAENQQDFFDFPLKAYMISEKCEVTLPAAVAVDGFFV
                     THARGWVELPPEDIKLPPRDSYHEAVPMMDNENPPARLSRDAPIQKSNFISYQMHASW
                     QQEIWAAVDRARPWVKKYMGGLIEVVNPGAETMIVASGSAVSQSREAVRQASERGDRI
                     GLVKIRSLRPWPTQELIEACKDAKRIIVPEFNCAGWLAKEVRSTLYGHSKAEIVSGPR
                     VFGGMSMPTELILEYLYKSPNVDRMTTKPLQA"
     gene            complement(1339..2076)
                     /locus_tag="AC16BC_05750"
     CDS             complement(1339..2076)
                     /locus_tag="AC16BC_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HIR3241158.1"
                     /translation="MATATGTARYQVLPGPEGLLPPSASAMGVVLPQDGEGLVEGVLV
                     SEDKALKELAKQMLTRRNPTIFPGPLVVWGWNKHTQEKASVVLELAAEIPGVKIIPMP
                     DYRPIYPKIDPEAVINPCHPNLTIWHNKIEACIFVGVHCHFANITLKMVRAGTNCYTV
                     ALCAEAGHEDAMASLPHCGIEKLQKVVKALRQVKKEGVTPWALTPEGKRELEAQREQK
                     RKQRETIAAAAPEFAHELVAGLDENEE"
     gene            complement(2337..3140)
                     /gene="panB"
                     /locus_tag="AC16BC_05755"
     CDS             complement(2337..3140)
                     /gene="panB"
                     /locus_tag="AC16BC_05755"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3241159.1"
                     /translation="MAPARVTVPELQRRKTDGERITMVTAYDYPFARIADEAGIDVIL
                     VGDSVGVVVQGHSTTLPVTMDQMIYHTEMVSRGARRAVVIGDMPFMSFQVSDQEAVRN
                     AGRFIQEGGAAAVKLESGPSMMSRVAAIAAADIPVMAHLGLAPQSIHKMGGYKVQGRT
                     PTAAQRLLDDAKRAEDAGAFAVLIEGVPPPVAKKITRSLSIPTVGIGAGPGCDGQVLV
                     LHDLLGLFVEFSPKFVKRYANLRETAVKALTAYRNDVMSGRFPSARESY"
     gene            complement(3140..4018)
                     /locus_tag="AC16BC_05760"
     CDS             complement(3140..4018)
                     /locus_tag="AC16BC_05760"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR3241160.1"
                     /translation="MRRLREVIEAGQFAVTVECEPPKGADVTTVLSRTKALLGRIHGI
                     NVTDNQAAVMRMSSLAMCRLLYEQGHDPVLQLTTRDRNRLALQSDLLGAHALGIRNVL
                     CLRGDDPTVGDHKDAKPVFDLDAAQLLGVVNGLNNGKDLAGNALKGATDFFPAAAVSP
                     EMVPPDQQLAGLEKKMAAGARFFQTQAVYNPDRFGAFMEKARPLGARILAGIVVLKSA
                     KMAEYMNKNIPGISVPQPLIDELAAADKEQALGVGIEIAVRLIGQIRGMCHGVHIMAV
                     GADQAIPEILSRANLG"
     gene            complement(4028..4897)
                     /gene="folD"
                     /locus_tag="AC16BC_05765"
     CDS             complement(4028..4897)
                     /gene="folD"
                     /locus_tag="AC16BC_05765"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR3241161.1"
                     /translation="MAAQLIDGKVISKAVKEQVRGRVEALKAKGIRPGLATVLVGDDP
                     ASHVYVRNKQRTCEELGMHTVGHNLPATTSQSELLTLIHRLNDDPAIHGILVQLPLPK
                     PLRSEPILNAMSPDKDVDGLHPFNMGKLLMGEPRFVPCTPGGVMAMLDYHKLPIEGKT
                     AVVVGRSNLVGKPAALLLLHRHATVTVCHSKTPNLAEVCRRADILVAAIGKAHFITGD
                     MVKAGAVVIDVGINRLPDGKLVGDVDFAAVAPKASWITPVPGGVGPMTIAMLMQNTML
                     AASMTADAGHHRH"
     gene            complement(4900..5514)
                     /locus_tag="AC16BC_05770"
     CDS             complement(4900..5514)
                     /locus_tag="AC16BC_05770"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR3241162.1"
                     /translation="MGAEDERERLRRAAVDRRNALAPASREARSRAITERFWDLPEVA
                     GARTLFVSLSVGSEVSTRALVAEARRRGLRVAVPVTLTAEQRLLPCDLPADDRLAAGP
                     FGILEPGPDARVPVAVDRLDLAVVPGAAFDPQGNRLGWGAGYYDRLLADLRPGVPIVA
                     LAFECQLVSVIPVEPHDIPVAVIVTEQRVIRPERSLRETHGKGE"
     gene            complement(5584..6909)
                     /locus_tag="AC16BC_05775"
     CDS             complement(5584..6909)
                     /locus_tag="AC16BC_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR3241163.1"
                     /translation="MDLFTPDATTSAETRPPLAERMRPRSLDEFVGQEHLVGKGRFLS
                     SVLQAEQPPSLIFWGPPGSGKTTLAHLIATSTKCRFVFFSAVLSGVKEVRELVAEART
                     RRQRGERTILFVDEIHRFNRNQQDAFLPHVEDGTIILMGATTENPSFEVNAPLLSRCK
                     VLTLRPLGVEQVVTLLRRAVNDKERGVGDLGVSCDDDLLAEIAEWAEGDARRALNLLE
                     QSIAFCPVTDGRRLLGAREVREAAQHRVLLFDKGREEHYNVISALIKSLRGSDPDAAV
                     YWLARLLESGEDPLFVARRMVIFASEDVGNADPQGLVIAVAALQAFHAVGMPEGWIPL
                     SHAATYLATAPKSNASYVAYRRAAAELDDTGTLPVPLHLRNAPTKLMKAEGYAEGYRY
                     PHDYPGHVVAQQYLPDQLVGRRFYDPSDVGYEREIGRRLDASRKDADEG"
     gene            7205..7540
                     /locus_tag="AC16BC_05780"
     CDS             7205..7540
                     /locus_tag="AC16BC_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR3241164.1"
                     /translation="MSRYRYHIFSCINKRSADDPKGSCSAKGSERLQELFKSEVKRRG
                     LQAEVRANKAGCLDACEHGPVVVIYPEGVWYRIRTDQDVLDVMNHHIERGQIVDRLVV
                     RFDGGSASA"
     gene            7588..>8334
                     /locus_tag="AC16BC_05785"
     CDS             7588..>8334
                     /locus_tag="AC16BC_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3241165.1"
                     /translation="MRTALALTAFYFASFAAVGIFLPYFNLYLLHLGFPPWQIGILAA
                     MPPLLKILVPAGFGLMADRTGGGRGLIIAMSAATTLTFACFLAATTFGSVLVVMVVFA
                     FVWAPVLPLVEATTLETVDRLKRFDYGRIRLWGSIGFIAATVAMGAWLKIAASDRVIL
                     FGILAGYAAATLAACGFPAARVATGAAELRPSGLSGLRPLWTFYAACLLMQFSHGTYY
                     GFFSIALEGRGWSASSIGALWAVGVAVEVLF"
BASE COUNT         1355 a         2881 c         2587 g         1511 t
ORIGIN      
        1 cgaaatcctt aatgtctcga gcgacatgcg tttcctcctg ttcggtgcct acccgctgcg
       61 ttacgcctgc agcggtttgg tggtcatacg atccacgttc ggcgatttgt acagatactc
      121 caaaatcaat tcggtcggca tcgacatgcc gccgaagacg cgcggcccgg acacgatctc
      181 ggccttcgag tggccgtaga gcgtggaccg gacttccttg gccagccacc ccgcgcagtt
      241 gaactccggc acgatgatgc gcttggcgtc tttgcacgcc tcgatcaact cctgggtcgg
      301 ccacggccgc aacgagcgga tcttcacgag tccgatgcgg tcgcctcgtt cgctggcttg
      361 gcgcacggcc tcccgcgact gcgacaccgc gctgccggac gccacgatca tggtctccgc
      421 gcccggattg accacctcga tcaggccgcc catgtatttc ttgacccacg gacgcgcgcg
      481 gtccaccgcg gcccaaatct cctgttgcca cgaggcgtgc atctggtagc tgatgaaatt
      541 gctcttctgg atcggcgcgt cgcgcgacag acgggccggc ggattctcgt tgtccatcat
      601 cggcacggct tcgtgatacg aatcccgcgg cggcaacttg atgtcctccg gcggaagttc
      661 cacccacccg cgcgcgtgcg tgacgaagaa cccgtccacc gccaccgcgg ccgggagcgt
      721 gacttcgcac ttctcggaga tcatgtaggc cttgagcggg aaatcaaaga agtcctgctg
      781 attctccgcg tgaaacacga ggatgccgca attgagcaga tacgagagtt cgatgttatc
      841 gggctggatg gccagcgggg cgttgatcac gcggcacatc acgagcaaca gcaacggcat
      901 gcggccgccc ggccacgacg tgatcacttc gaggccacgc atcaacccag ggccggcggt
      961 cgccgtcagc gcccgaacgc ctgaacgcga ggcgcccgcg atcgcggaca tcacgccgta
     1021 ttcttcctcc ccgcggtagt aatccttgag atacccttcg gcatacagat acccgacctg
     1081 ctgcatcgtt tcgctctgcg gcgtgatcgg gtaggaaatc gcgatgtcga cgttggcgcg
     1141 gcgaatggct tctttcgcgg cctcgctgcc cgtgatgaac gtcttggtcc gcggcgcctc
     1201 gaaaaacaga tgttccgggg tcaccacccg ttgtttggcc gccttgtttt ctttttcctt
     1261 cttgggcgct tcggtcgcgg taatgacgtc gccctggggg ttggttttga cttcggtctc
     1321 ggtcccgtca gccatcgctt actcctcgtt ttcgtccagc ccggcgacca actcgtgggc
     1381 aaactccggc gccgcggcgg caatggtctc gcgctgtttc cgcttctgct cgcgttgagc
     1441 ctccagttcc cgcttcccct ccggagtcaa cgcccagggc gtcacccctt ccttcttgac
     1501 ctgacgaagc gccttgacga ccttctgcag cttttcaatg ccgcagtggg ggagcgacgc
     1561 catggcgtcc tcgtgcccgg cttccgcgca caacgccacg gtgtagcagt tggtccccgc
     1621 ccgcaccatc ttgagggtga tgttcgcgaa atggcagtgc acgccgacga agatgcacgc
     1681 ctcgatcttg ttgtgccaga tggtcaaatt gggatgacac ggattgatca cggcctcggg
     1741 atcgatcttg ggatagatcg gccggtagtc cggcatcgga atgatcttga cgccggggat
     1801 ctccgccgcc agttccaaca ccacgctcgc cttctcctgc gtgtgcttgt tccagcccca
     1861 gaccacgagc ggaccgggga aaatggtggg gttgcgccgg gtcagcatct gcttcgccaa
     1921 ctctttgagc gccttgtcct cggatacgag gacgccctcg accaggcctt cgccgtcctg
     1981 cggaagaacc acgcccatcg ccgacgcgga gggcggcagc aagccctcag ggcccggaag
     2041 gacctgatag cgcgcggtgc cggttgcagt agccatacgg atcaactcct ctctactcta
     2101 cccgttgaac tgagacgttg acgcgagaac acggcccaag cacttcagag gccgtttcgt
     2161 gggccctacc atatcgaatt ccgaagcccg cgtcaattca gcagaagacc tagcgaccgg
     2221 ctaggacttt cggcgcatcg gaaccgtttc agcggaaagc gagtcgtact atatcgaccc
     2281 ggcaaaacgg ttgtcaacga acaccgggcc gccgcgacgc aatcatcggc tccggctcaa
     2341 taactctcgc gcgcggacgg gaaccggccg ctcatcacat cgttccggta cgcggtcagc
     2401 gccttcaccg ccgtctcgcg gagattggcg tagcgtttca cgaacttggg gctgaattcg
     2461 acgaacaacc cgagaagatc gtgcaacacc aacacctgcc catcgcatcc gggtcccgcg
     2521 ccgattccca ccgtggggat ggacagagac cgcgtgatct tcttggcaac cggcggcggc
     2581 acgccttcga tcaacacggc aaaagcgccc gcgtcttccg cgcgtttggc gtcgtcgagc
     2641 agacgctgag cggccgtggg cgtccggccc tgcaccttgt accctcccat cttgtgaatg
     2701 gactgcggcg ccaatcccag atgcgccatc acgggaatat cggcggccgc gatcgcggcg
     2761 acacgcgaca tcatggaggg accgctctcc agtttcaccg cggccgcgcc gccctcctgg
     2821 atgaaccggc ccgcgttgcg caccgcctcc tggtcggaca cctgaaacga catgaacggc
     2881 atgtcgccga tgaccaccgc ccgccgtgcc ccgcggctca ccatctccgt gtggtagatc
     2941 atctggtcca tggtgaccgg gagcgtggtg ctgtgccctt gcaccaccac gccaacggag
     3001 tcccccacca agatgacgtc gatgccggcc tcgtccgcga tgcgtgcgaa cggatagtcg
     3061 tacgcggtca ccatggtgat ccgctcgccg tcggtcttgc gccgttgcag ttcgggaacc
     3121 gtgacgcggg cgggcgccat caaccgaggt tcgcgcggga gaggatttcc gggatggcct
     3181 gatccgcgcc cacggccatg atgtgcacgc cgtgacacat gccgcggatc tggccgatga
     3241 gccgcaccgc gatttcgatc ccgacgccca gcgcctgttc cttgtctgcg gcggccaact
     3301 cgtcgatgag cggctggggc accgaaatcc ccggaatgtt cttgttcatg tactccgcca
     3361 tcttcgcgga cttgaggacc acgatgccgg ccaggatgcg cgcgccgagc gggcgcgcct
     3421 tttccatgaa cgcgccgaac cggtcgggat tgtagaccgc ctgcgtttga aagaaccgcg
     3481 cccccgccgc catttttttc tcgagcccgg ccagttgctg gtcgggcggg accatctcgg
     3541 ggctgaccgc ggccgccggg aaaaagtcgg tcgcgccctt gagcgcattg ccggccagat
     3601 ccttgccgtt gttcagcccg ttcaccacgc ccaggagttg tgcggcatcc agatcgaaca
     3661 ccggtttggc gtccttatgg tcgcccacgg tggggtcgtc gccgcgcagg cagagcacgt
     3721 tgcggatgcc caatgcgtgc gcgcccagca ggtccgactg gagcgccagg cggttgcgat
     3781 cccgcgtggt caactggagc accgggtcat ggccctgctc gtagagcaac cggcacatgg
     3841 ccagcgacga catgcgcatc acggccgcct gattatccgt gacgttgatg ccgtggatgc
     3901 gccccagaag cgccttcgtc cgggacaaca ccgtcgtgac atccgcgccc ttgggtggtt
     3961 cgcactcgac cgtgaccgcg aactggccgg cctcgatcac ctcgcgcaat cgccgcatac
     4021 tctctcctta gtgccgatga tgacccgcgt ccgcggtcat ggacgcggcc agcatcgtgt
     4081 tctgcatcaa catcgcaatc gtcatcggcc ccaccccgcc aggaaccggg gtgatccacg
     4141 acgccttggg cgcgaccgcc gcgaagtcca cgtcgccgac cagtttcccg tcgggcagcc
     4201 ggttgattcc cacgtcgatc accactgcac ccgctttgac catgtcgccg gtgatgaagt
     4261 gcgccttccc gatcgccgcc accaggatat ccgcgcgccg acagacctcc gcgagattcg
     4321 gcgtcttgga gtgacagacg gtcacggttg cgtgtcggtg taacaacaac aatgccgcgg
     4381 gtttgccgac caggttgctg cggcccacca ccaccgcggt ctttccctcg atcggaagct
     4441 tgtggtagtc gagcatggcc atcacgccgc cgggcgtgca cggcacgaac cgcggctcgc
     4501 ccatcagaag cttgcccatg ttaaacgggt gcaggccgtc cacatccttg tccggggaca
     4561 tggcgttgag gatcggctcg ctgcgcaggg gtttgggcaa cggcaattgc accaggatgc
     4621 cgtggatcgc gggatcgtcg ttcaaccgat ggatcagcgt cagcaactcg ctctgcgagg
     4681 tggtggccgg cagattgtgg ccgaccgtat gcatcccgag ttcttcgcac gtgcgctgct
     4741 tgttccgcac atacacgtgc gaggccggat cgtctcccac caacacggtc gccaaccccg
     4801 ggcggatgcc cttggctttc aacgcctcga cgcgcccgcg aacctgttct ttgaccgcct
     4861 tggaaatgac cttgccgtcg atcaactgcg ccgccacgtt cactcccctt tcccatgcgt
     4921 ctcccggagg ctgcgctccg gacgaatcac tcgttgttcg gtcacgatca cggccaccgg
     4981 gatatcgtgc ggttcgaccg ggatcaccga cacgagttgg cactcgaatg ccaacgccac
     5041 aatcggcacg ccgggacgaa gatccgccaa caatcgatcg taatatccag ccccccaccc
     5101 caaccgattg ccctgggggt cgaacgccgc gcccggcacc accgccaggt cgagccggtc
     5161 aaccgcaacc gggacgcgtg cgtccgggcc gggttcgagg atgccgaacg gccccgccgc
     5221 caaccggtcg tcggccggaa ggtcgcacgg cagcaagcgt tgttccgcgg tcagcgtcac
     5281 cggtaccgcg acgcgcaggc cgcgccgcct ggcctccgcc acgagcgcgc gggtcgagac
     5341 ctcgctgccg accgacaacg acacgaacag cgtgcgcgcg cccgccacct cgggcaagtc
     5401 ccaaaaccgc tcggtgatcg cgcggcttcg cgcttcgcga ctcgccggcg ccagcgcgtt
     5461 gcgtcggtca accgcggccc ggcgaagccg ctcgcgctcg tcttcggcac ccaaagccgc
     5521 gccagaatag caaggccccc gcgaacaatc aagcgcccgt cccgcggggg gccgggcgcg
     5581 accttacccc tcgtccgcgt cctttcgcga ggcgtcgaga cggcgcccga tctcgcgctc
     5641 gtagccgacg tcgctgggat cgtaaaaccg ccgtccgacc aattgatccg gaagatactg
     5701 ctgggcgacc acgtgccccg ggtagtcgtg cgggtaccga tacccttccg cgtacccttc
     5761 ggccttcatc agtttggtgg gtgcgttgcg cagatgcagc ggcaccggaa gcgtgccggt
     5821 gtcatcgagt tccgcggcgg cgcgacggta cgcgacgtag ctggcgttgc tcttgggcgc
     5881 ggtcgccagg tacgtggcgg cgtggctgag gggaatccag ccctcgggca tgcccaccgc
     5941 gtgaaacgcc tgcagcgcgg ccaccgcgat gaccaatccc tgcgggtccg cgttgcccac
     6001 gtcttccgac gcgaagatca ccatgcgccg cgccacgaac aacgggtcct ctccggactc
     6061 gagcaggcgc gcgagccaat acaccgcggc gtctgggtcg ctgccgcgca ggctcttgat
     6121 gagcgcggag atgacgttgt agtgctcctc gcgccccttg tcaaagagca acacgcggtg
     6181 ctgcgcagcc tcgcggacct cgcgcgcgcc gagcagccgg cggccgtccg tgaccggaca
     6241 gaacgcgatc gactgctcca gcaggttcag cgcccgccgc gcgtcgccct cggcccattc
     6301 cgcgatctcg gccaacagat catcgtcgca cgacacccca agatccccca cgccccgttc
     6361 cttgtcgttc acggcccgtc ggagcagcgt caccacctgc tccacgccca agggcctgag
     6421 cgtcaggact ttgcagcgcg agagcaacgg cgcgttcacc tcgaagctgg gattctccgt
     6481 ggtcgcgccc atgagaatga tcgtgccgtc ttccacgtgc gggaggaacg cgtcctgttg
     6541 gttgcggttg aaccggtgga tttcatccac gaacaggatc gtgcgctcgc cccgctgccg
     6601 ccgcgtccgc gcctcggcca cgagttcgcg cacttccttg acgcccgaca acacggcgga
     6661 gaaaaacacg aaccgacact tggtgctggt cgcgatcagg tgcgcgagcg tggtcttgcc
     6721 cgagcccggc ggcccccaga aaatcaacga cggcggctgc tccgcctgga gcaccgagga
     6781 cagaaaccgc cccttgccga ccaggtgttc ctgcccgacg aattcatcca ggcttcgcgg
     6841 ccgcatccgc tccgcgagcg gcggtcgggt ctcagcggag gttgtggcgt cgggagtgaa
     6901 caggtccata ggagtcggca tgctaccaca gagctcggcc cgcccgccaa ccccgcaacg
     6961 gggatttccg aggctccgat cctcgtctgc gactcgctga gcgcggttga ggcaagcagg
     7021 gagcgcccac ccgatgtttt cgccaggtcc tgtatccgca gcaagcgcgc ctagggggag
     7081 cgccacgaca gaccgcggca gttccgccgc gcggccccag acgtgtgccc cctcaggtgg
     7141 taacaccgcc gcagcgcccc tcgccgtgct ataatccgcg ccactttcca cacgggaggc
     7201 gcggatgtct cggtaccggt accacatttt ttcctgtatc aacaaacgct ccgcggacga
     7261 ccccaagggc agttgctcgg ccaagggatc ggaacgattg caggagttgt tcaagtccga
     7321 agtgaagcgt cgcgggcttc aggccgaagt gcgcgccaat aaggccgggt gtctggacgc
     7381 gtgcgagcac gggcccgtgg tggtgatcta ccccgagggc gtgtggtatc ggatccgcac
     7441 cgaccaggac gtgctcgacg tcatgaatca ccatatcgag cgcggccaga tcgtggatcg
     7501 cctcgtggtc cggttcgacg gcggttccgc ctcggcctga tcgtgtctcg ccattgcggc
     7561 gcgcgccgcg cgggaaaggc ctgagacgtg cggaccgcgc tggcgctgac ggcgttctac
     7621 ttcgcgtcgt tcgcggccgt cgggatcttc ctcccctact tcaatctcta cctgctccac
     7681 ctgggcttcc ccccctggca gatcggaatc ctggcggcca tgccgccgct gctgaagatc
     7741 ctcgtgcccg cgggcttcgg actcatggcg gaccgcacgg gcggcgggcg cgggctgatc
     7801 atcgccatgt ccgcggccac cacgctcacg ttcgcgtgtt tcctcgcggc caccacgttc
     7861 gggagcgtgc tggtggtcat ggtcgtgttc gcgtttgttt gggccccggt gctcccgctg
     7921 gtggaagcca cgaccctgga gaccgtggac cggctcaagc ggttcgacta cggccgtatc
     7981 cggctgtggg gatcgatcgg tttcattgcg gccacggtcg cgatgggcgc gtggctcaaa
     8041 atcgcggcgt cggatcgggt cattctcttc ggcattctcg ccgggtacgc cgcggccacg
     8101 ctggccgcct gtggattccc cgccgcgcgc gtcgccacgg gcgccgccga actgcgtccg
     8161 agcgggctca gcggcttgcg gcccctctgg acgttctacg ccgcgtgcct gctcatgcag
     8221 ttcagccacg ggacgtacta cgggttcttt tcgatcgcgc tggagggccg cgggtggtcc
     8281 gcgtcgtcga tcggcgcgct ctgggccgtg ggcgtggccg ttgaggtgct cttc
//
LOCUS       DBNGTT010000258         7091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2101710, whole genome shotgun sequence.
ACCESSION   DBNGTT010000258 DBNGTT010000000
VERSION     DBNGTT010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2101710"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="AC16BC_05790"
     CDS             complement(<1..979)
                     /locus_tag="AC16BC_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241166.1"
                     /translation="MTVTRGRTPWSLLGVIGALTALVIAAHGGALRGTFHYDDLFAVV
                     NNPAVRSWDPVRIARSTEAVNSERNASGYRPLTVGSLALNYRLSGLDASGYLATNLLL
                     HVLASALVVLLARELLGDLRWAAAAGAVFALHPVNAEAVNYVTARSSLLSTVFALAAA
                     WAFVRDAEGRAGVGGRAAGLVAFLCALLSKESAVALAAPVLVYPWLFRRRPSDAKAFS
                     RGVRAAAGYGVVAAGFVAGFGLLTAGGTTVLVPGQRPAWTFLEMVGRSMALWVWPWPL
                     GLDHPLTFLSRFDAVLAAVLLIGAAGFVAAVLWLRARAPAAAWGMIWAAA"
     gene            complement(976..2625)
                     /locus_tag="AC16BC_05795"
     CDS             complement(976..2625)
                     /locus_tag="AC16BC_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241167.1"
                     /translation="MNVQARWTRGATLAGLLLAVLAAYAGAITNGFLYDDYHLIAENP
                     AVITHAWGAVWTSGDAASRDIAGRGFRPVTLSSYLLDHAVGSGRPLVYHATQVGLHAM
                     VVGLVYLVATGVGFRLPWAAAAGLLMGLHPVQVEAVHYLSARSSVLSTLWLLAAMWSY
                     LRGREYPGVRNRWVTASLACLALAVWSKESAVVGIVWFVAYERIVAGATWGEVTRRVW
                     LHAAVGALSVLPAVVVLRGGGGGALVEVGAATATGVHAIGRHAWAMLVPMNLGSVSPQ
                     PWVGWAQPAVWASVALVLAICAAGYALRTRVPRFAWGVVCGVSGLLPVLALPFVTNVG
                     LVQPHRGYQAMAGFALAIAACLEAVADRLRTLVRSEAGRRALRNALLAAAGVGTVVLI
                     AADVNAGRSWRDEVGFWTRAVEQYPGEAGYHQSLGAARLRAGDAPGALDALTRAATLD
                     PALPRVSYNLGLVYTKLGRIDEAIAAYERAVSADASDVKALANLGWLFEKRGERDRAL
                     ASYRAALRVAPDLARVRDRVAALDPAMSAHEAGSPNGVGRP"
     gene            complement(2849..>7091)
                     /locus_tag="AC16BC_05800"
     CDS             complement(2849..>7091)
                     /locus_tag="AC16BC_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3241168.1"
                     /translation="RPIRTLSTGPRSIALLLAAGLLAACGAEDFPGAPGGAGDLNVPY
                     VTTYNFGFNVGFTPIGGVAGNLLIISSGDLINLVSAANITRLPATGVISGRALTTDQG
                     ISATNVELVAMDVNGRPVGAFFYNSLGGTPDFVQTRGTTAAGGFTAFNVPPGEVYLSA
                     VGGGRGAARLVAFPDEVSLGTVMITPVVVPIVGVLGEVSDGAGQPVKSQHNIEVGVVG
                     QRLSEAINCPDEIPTQDRVGAIFVESTSGNKTSFRFCVASDGDYFARLTGDSSYVPTY
                     QTFNSDSSRLAALQTAELTEFFTIQDRAGLDQAAAAAGTTWDPSKGVLAGQVQYGAGA
                     ARSAAVVRITDEDGNEVAVAGGGDPDKGAIIYLDLLGIPRTGPDAFSTSGSGRFIALN
                     IPLPPGQQSDTVYVTVTAERPTAGVKERYAATSIVPVMAGAVIYDIISTVSLLPPIQG
                     EDQNEAFFTGPVSGRVMREDGLAPVAGGRVTPLGVPLAELPVPRPDPNAPQEFFPIDA
                     NGRYEVLINPDDSATTPLLQSNVYLVRVEGPTGSDYLPTYQRIKTGSAEIDETTGLVK
                     TQTQNLVAVTQSAIDGYRAAVGQGPAVPGTGVLLGTVVDTKTGLPTGGVSIRLTAVDG
                     LDASTSPSGQSASIYYFDFGGVPRTDLNATTPNGRFIVFDAPVGAVSLDVTSSDDTGN
                     VLADSRADGVTVVRIDANNAPPDVVEVSGKAVSLAGSLPTGALTVTAAGGDPMTVRRL
                     CPDTAFERGGVCYDSANPSQIVPKTIVPCTDEATYGKYRVLKDGYCQLQYTAVSAAAP
                     FTIPVGSLRDMVLKTSGSGLVDTYTFGLSIGSRPMTDVSLGTVTASGLGAMAASAGRA
                     LSPGNGVIFGQATTASLGEVDDQGSVVPHTCSQPVPWTRLPDNAAVGCGALGTPGALV
                     TGDFNDDTYRDLAIIDTAASDPGVTIWLNTGDGGFVRSQRIVKRFDCAIGDVGCGVED
                     GPVALLVVDYNGDGTSDIVVLNEQSRSISLLEGRGAGQFVFGRSQVISDAGTDPDTLV
                     GADFNLDRIPDLVITERVSGRIILLLGNGAGFRTPPSTVQAPQVGNDPRAVLSGHLDF
                     DGIPDLVFVNRDSISVMQRIGVANTLTSYPVPSGSTADLSAAAIGDVDGNVFNDIVVA
                     DRSAGDPRIWVFLNGFTGLQAPTAIAAGAQPVAIAVTDLNGDRSADLMVVHQSDGSLM
                     YREGDGGGGFGPERVFRLGGQPRGLVMDDVTGDGAPDAILSDPAPGAARVIILPHSTR
                     PVDGVAVAAVRADGRAVGEIVYLDQQDQPMVGATLTGTSGRFALLNVPPGPVWLRLMT
                     GGLGSRLLYAYPDAVSNTKFPVVTGLNATTTVSGVTADAVLRPVGEVQIRFLGTQRAT
                     SSIPLEFERNGNTVGGASYAAVVEANSEYVVKLSR"
BASE COUNT         1257 a         2581 c         2247 g         1006 t
ORIGIN      
        1 ccgcggcggc ccagatcatc ccccacgcgg ccgccggcgc ccgcgctctc aaccacagaa
       61 cggccgcgac gaacccggcc gcgccgatca gcagcacggc ggccaacacc gcgtcgaaac
      121 gcgagaggaa ggtcaggggg tggtccaacc ccagcggcca gggccacacc cagagcgcca
      181 tcgagcgtcc caccatttcg aggaacgtcc aagccggccg ttgcccggga acgaggacgg
      241 tcgtgccccc ggcggtcagc aacccaaacc ccgccacgaa ccccgcggcc accaccccgt
      301 atcccgccgc ggcccgcacc ccgcgcgaga acgccttggc gtcgctcggc ctccggcgaa
      361 acagccacgg gtagacgagc acgggcgccg cgagcgccac cgcggactcc ttactgagca
      421 gcgcgcagag aaacgcgacg agtccggccg cgcggccgcc aacgcccgcc cggccctcgg
      481 cgtcacgcac gaacgcccac gccgcggcca gcgcaaagac cgtggaaagt agggacgaac
      541 gcgcggtgac gtagttgacc gcttcggcgt tgaccggatg gagcgcgaag accgcccccg
      601 ccgccgcggc ccaccgcaga tcgccgagca actcgcgggc gagcaggacg accagcgccg
      661 acgcaagaac gtgcagcaac aggttcgtcg ccaaataacc cgaggcgtcg agaccggaca
      721 ggcgatagtt gagggccagc gaacccaccg tcaacggacg gtacccggac gcgttccgtt
      781 cactgttgac cgcctcggtc gagcgcgcga tgcgcaccgg gtcccaggac cgcaccgccg
      841 ggttgttgac caccgcgaac aagtcgtcgt agtgaaacgt tccgcgcagc gcgccgccat
      901 gcgcggcgat caccagggcg gtcagcgcac cgatcacgcc gagcaggctc catggcgtgc
      961 gcccccgcgt cacggtcatg gacgcccgac tccattcggg ctcccggctt cgtgggcgct
     1021 catcgccgga tcgagcgcgg ccacgcgatc gcgcacgcga gccagatccg gcgcgacccg
     1081 gagcgccgcg cggtaggacg cgagggcgcg atcgcgttcg ccgcgtttct cgaacaacca
     1141 tccaaggttg gccagcgcct tgacgtccga cgcgtccgcg gacacggccc gctcatacgc
     1201 ggcgatcgcc tcatcgattc gaccgagttt ggtgtacacc agccccagat tgtatgacac
     1261 ccgcggcagc gcggggtcga gcgtcgccgc acgggtcaac gcgtcaaggg cgcccggcgc
     1321 gtcgccggcg cgcaggcgcg cggctccgag gctctgatga tacccggcct ccccgggata
     1381 ttgctccacg gccctggtcc aaaacccgac ttcgtcgcgc caggaccggc cggcgttcac
     1441 atccgcggcg atcaggacca ccgtgccgac ccctgcggcg gcgagcagcg cattccgcag
     1501 cgctcgtcgc cccgcctccg aacgcaccag cgtccgaagg cggtcagcca cggcctcgag
     1561 gcatgcggcg atcgccagcg cgaatcccgc catcgcctga tagccccggt ggggttgcac
     1621 cagtccgacg ttggtcacga acggcagcgc caacaccggg aggaggccgc tcactccgca
     1681 gacaactccc cacgcgaacc ggggcacgcg ggttcggagc gcatacccgg ccgcgcagat
     1741 cgcaaggacc agggccacac tcgcccagac cgcgggctgc gcccacccga cccacggctg
     1801 cggggacacg gacccgaggt tcatcggaac cagcatcgcc cacgcgtgcc ggccgatcgc
     1861 gtgcacgccg gtggccgtgg ccgcgccgac ctccaccagg gccccgcctc cgccgccgcg
     1921 caggacgacc acggcgggca acacgctgag cgcgcccacg gccgcgtgaa gccagacccg
     1981 ccgcgtgacc tcgccccacg tcgcgccggc cacgatccgc tcgtacgcga cgaaccagac
     2041 aatgcccacg accgcacttt ctttgctcca caccgccaag gccaggcacg ccagactcgc
     2101 ggtgacccat ctattccgga cacccggata ctctcggccg cgaagatacg accacatggc
     2161 ggccaagagc cacaacgtac tgagaaccga cgaacgcgcg ctgagatagt gaacggcttc
     2221 gacctgaacc gggtgcaggc ccatcagcag cccggccgca gccgcccagg gaagcctgaa
     2281 cccgacgccc gtggccacca gatagaccag accgacaacc atggcgtgga ggccgacttg
     2341 ggtggcgtga tagaccaacg gccgcccact ccccaccgcg tggtcgagaa gatatgagga
     2401 gagcgtaacc ggacgaaacc ctcggcccgc gatatcccgg ctggcggcgt ccccggacgt
     2461 ccacaccgcc ccccaggcat gcgtgatgac cgcggggttc tcggcgatca ggtggtaatc
     2521 atcgtagaga aaaccgttcg tgatggcccc ggcgtacgcg gccaggaccg caaggagcag
     2581 tcccgccaat gtggcccctc gggtccagcg cgcctgaacg ttcacgcgtc agcctagagg
     2641 agggaagggg aggagggatg tcgcgattcc ccgtccatgc ctgacaatcg gagaccggga
     2701 gccgtgacct ctggagtgaa cgccggcgag gtcccgtgac tgactgaacc gcgaaaccgc
     2761 ctagtgccgc ggcggaccgg caacggccgc agaaggctca tgacaactcc ccgttcatca
     2821 ctccacgcct accgccgctt gtgacctgct acctgctcaa tttgaccacg tactcgctat
     2881 tggcctcgac cacggccgcg taacttgctc cgccgaccgt attgccgttt cgttcgaact
     2941 ccaacgggat ggaactggtg gcgcgctgcg ttcccaaaaa ccggatctgc acctcgccca
     3001 ccggtcgcaa gacggcatcc gcggtgaccc cgctgacggt cgtggtggcg ttcagccccg
     3061 tgacgaccgg aaacttggtg ttggacacgg cgtccggata cgcgtacagc aaccggctgc
     3121 ccaaaccgcc ggtcatcaat cgaagccaga ccgggcccgg gggaacgttg agcagcgcaa
     3181 accggccgga ggttccggtc aacgtggcgc cgaccatcgg ttgatcctgc tggtcgagat
     3241 aaacaatctc gccgacggcg cggccgtccg ctcgaaccgc cgccacggcc acgccgtcga
     3301 ccggccgtgt actgtggggg aggatgatca cacgggcggc gcccggcgcc ggatcggaga
     3361 ggatggcgtc cggcgcgccg tcgccggtca cgtcgtccat caccaagccg cgcggctgtc
     3421 cgccgaggcg gaacacccgt tccggtccga acccgccacc tccgtcaccc tctcgataca
     3481 tcagggatcc gtcgctttga tgcaccacca tgagatccgc ggaccggtcc ccgttgaggt
     3541 cggtcaccgc gatcgcaacc ggctgggccc ccgccgcgat cgcggtgggg gcttgcagcc
     3601 ccgtgaaacc gttgaggaac acccagatcc tgggatcacc ggccgaccga tcggcgacca
     3661 cgatgtcgtt aaacacgttc ccgtccacgt cgccgatcgc ggccgcgctc aaatccgcgg
     3721 tcgaaccgga tggaaccgga tacgaggtca atgtgttggc caccccgatg cgctgcatca
     3781 ccgaaatgct gtcgcgattc acgaagacca gatccggaat tccgtcgaaa tccaggtggc
     3841 cggacaacac ggcgcgcgga tcgttcccca cctgcggcgc ctgcaccgta gacggcggcg
     3901 tccggaagcc cgccccgttg cctaacagca aaatgatccg gccgctgacc cgctccgtga
     3961 tgacgaggtc cgggatccga tccagattaa agtccgcgcc gaccagggtg tcggggtcgg
     4021 ttccggcatc actgatcact tggctccggc cgaacacgaa ctgaccggcg ccacggccct
     4081 ccaacaggga gatactccgc gactgctcgt tgagcaccac gatgtcggaa gtcccgtcgc
     4141 cgttgtagtc caccaccagc agggctaccg gcccgtcctc cacgccgcag cccacgtcgc
     4201 cgatcgcgca atcgaatcgt ttcacgatgc gctgggaccg cacgaaccct ccgtccccgg
     4261 tgttgagcca gatcgtaacg ccggggtcgg acgccgccgt gtcgatgatg gccaggtccc
     4321 ggtacgtgtc gtcgttgaag tcgccggtga ccagcgcgcc gggcgtcccc agcgcgccgc
     4381 accccacggc ggcgttgtcg ggaaggcgcg tccagggcac cggctgactg cacgtgtgcg
     4441 ggacgacgct cccttggtcg tcgacttcgc ccaggctcgc cgtggtcgcc tgtccgaaaa
     4501 tcaccccgtt gcccggcgac agtgcccggc cggccgaggc cgccatggct cccaacccgg
     4561 aggccgtcac cgttcccaat gacacgtccg tcatcgggcg agagccgatc gacagaccga
     4621 aggtgtaggt atccacaagc ccgctcccgg acgttttgag caccatgtcg cgcaaggaac
     4681 cgaccggaat cgtaaacggc gcggcggccg agacggcggt gtactgcaat tggcagtacc
     4741 cgtccttcaa cacacggtat ttgccgtagg tcgcttcgtc cgtgcagggc acgattgtct
     4801 tgggaacgat ctgactgggg ttcgcggaat catagcagac gccgccgcgc tcgaacgcgg
     4861 tgtcggggca caagcgccgg acagtcatcg ggtcgccgcc ggccgcggtc acggtcaagg
     4921 ctccggtcgg caatgatccc gccagggaga ccgccttgcc cgacacctcc accacgtccg
     4981 gcggcgcgtt gttcgcgtcg atccgcacca cggtgactcc gtccgcccga ctgtcggcca
     5041 gcacattgcc cgtatcatcc gaggaagtca cgtccaacga caccgcgccg acgggagcat
     5101 cgaacacgat gaagcggccg ttcggcgtgg tggcgttcag gtcggtgcgc ggcaccccgc
     5161 cgaaatcaaa gtagtagatg gacgcggact gcccgctcgg gctcgtactc gcatccaagc
     5221 catccacggc ggtcaaccga atcgagacgc cccccgtggg caggcccgtc ttggtgtcca
     5281 cgaccgtgcc caacaacacg ccggtcccgg gaaccgccgg gccttggccc acggccgccc
     5341 ggtacccatc gatcgcgctc tgcgtcaccg caaccaggtt ctgtgtctgc gtcttgacga
     5401 gcccggtggt ttcgtcgatt tcagcgcttc cggtcttgat gcgttggtac gtcggcaggt
     5461 aatcggatcc ggtgggccct tccacccgga ccaggtagac gttggactgc aagagcggcg
     5521 tggtcgcgct atcatccggg ttgatcagga cttcgtaacg gccgttcgcg tcgatcggga
     5581 aaaactcctg gggggcattc ggatccggac gcgggaccgg caattccgcc aacggcactc
     5641 ccagcggggt gactcgccca ccggccaccg gcgccagtcc gtcttcccgc atcacgcgcc
     5701 cggacaccgg gcccgtaaag aacgcttcgt tttgatcctc gccttggatc ggcggcagca
     5761 gcgacaccgt gctgatgatg tcatagatca ccgcgcccgc catgaccggc acaatcgatg
     5821 tggcggcgta ccgttccttc acgccggcgg tggggcgttc ggcggtgacg gtcacgtaaa
     5881 ccgtgtccga ctgctgtccg gggggcagag ggatgttcaa cgcgatgaag cggccggacc
     5941 cgctcgttga aaacgcgtca ggtccggtcc gcgggatccc caggagatcc agatagatga
     6001 tggcgccttt gtccgggtcg ccgccgcccg ccaccgcgac ctcgttgccg tcttcatccg
     6061 tgatccgcac cacggccgcg ctccgcgccg ctccggcccc gtactggacc tgcccggcca
     6121 agacgccttt ggacggatcc caggtggtcc ccgcggcggc cgcggcctga tccaggcccg
     6181 cccgatcttg tatcgtgaag aactcggtca actccgcggt ctggagcgcg gcgagccgcg
     6241 aactgtccga attgaacgtc tggtaggtcg gaacgtacga cgagtccccg gtgagccgcg
     6301 cgaagtaatc cccgtcggac gccacgcaga accggaagct ggtcttgttc ccggacgtgg
     6361 actccacgaa aatcgcaccc acgcgatcct gggtggggat ctcatccggg caattgatgg
     6421 cctcgctcag gcgctggccc accaccccga cctcgatgtt gtgctgactc ttgaccggtt
     6481 gcccggcgcc gtcgctgact tcccccagca cgccgacgat cgggaccacc acgggcgtga
     6541 tcatcaccgt gcccaacgag acttcgtccg gaaacgccac cagacgcgcc gccccccgac
     6601 ctccgcccac ggcgctgaga tacacttcgc cgggcggtac attgaaagcg gtgaacccgc
     6661 ccgccgcggt cgtgccgcgc gtctgcacga aatccggcgt gcccccgagg ctgttgtaaa
     6721 agaatgcgcc gaccggtcgg ccgttcacgt ccatggcgac caactcgacg ttcgtcgcgg
     6781 agatgccctg atccgtggtc agcgcgcgcc cgctgatcac gcccgtcgcg ggaagtctgg
     6841 tgatattggc cgccgacacg aggttgatga ggtcgccgct ggaaatgatc agcaggttcc
     6901 cggcgacgcc gccgatcggc gtgaatccca cgttgaatcc gaagttgtag gtggtcacat
     6961 acggcacgtt caagtcgccg gccccgcccg gggcgccggg gaaatcttcc gcgccgcagg
     7021 ccgccagcag tccggccgcc aacagcaggg cgatcgatcg gggccccgtc gagagggttc
     7081 tgatcggcct c
//
LOCUS       DBNGTT010000259         2000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1845772, whole genome shotgun sequence.
ACCESSION   DBNGTT010000259 DBNGTT010000000
VERSION     DBNGTT010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1845772"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC16BC_05805"
     CDS             complement(<1..194)
                     /locus_tag="AC16BC_05805"
                     /inference="COORDINATES: protein motif:HMM:NF026066.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HIR3241169.1"
                     /translation="MTNAEIARVMREIAMFLDMEGDRFRPRAYEKIAHALEALDEPAA
                     DVYRRGGVKALAEIPGVGTHT"
     gene            complement(287..748)
                     /locus_tag="AC16BC_05810"
     CDS             complement(287..748)
                     /locus_tag="AC16BC_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HIR3241170.1"
                     /translation="MTRPSVSEPRPMSSPDQPEYPFPLVLYDGACNLCVGSVRFVIAR
                     DSRGRVRFASMQSPIGQRLLARFGLPGDEFKTFVLVEAGKHFTRSAAALRTARYLDGW
                     WPWLYALIAVPRPIRDAVYDRVARNRYRWFGRRESCLVPDPGLRDRFIDAP"
     gene            843..>2000
                     /locus_tag="AC16BC_05815"
     CDS             843..>2000
                     /locus_tag="AC16BC_05815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3241171.1"
                     /translation="MTDTRTVDCLIIGGGIAGTTAAEGLRKKSAARIMIVTEEPHPLY
                     SRVRLPDYLVAQIPRERVFMKDEAWYRERGIDLVRGRSVHALSTADRSVRLDDGSTVR
                     YGALLLAMGGAARRLSCEGADWTGVHYLRTIDDADRIRSSIPAATRAVVVGGGFIGLE
                     LARCFAHHNRPTTMVLMEPRFWPAFFDDTANAWVDRTLRSRGIDVRYSEQLQSIRGAG
                     AVRDAVMASGNAFPCDIMGVGIGISTMHPFVKDSGLAIRKGIVTDEYFRTTDPHVFAA
                     GDVAEFFDLTCGRSNQLGNWSNAMEQGKVAALNMAGEPTPYRFVGGYVIAIFGLPVGL
                     TGDPSTPQGSEVILRGSPDRGSYARLIVRDGVIKGAAVLNAPKENVTVGQLI"
BASE COUNT          333 a          708 c          665 g          294 t
ORIGIN      
        1 gtatgcgtcc cgaccccggg aatctcggcc agcgctttga ccccgccgcg tcggtagacg
       61 tcggccgcgg gttcgtcgag cgcctccagc gcgtgcgcga tcttctcgta cgcccgcggc
      121 cggaaccgat cgccttccat gtcgaggaac atggcgatct cgcgcatgac gcgggcgatc
      181 tcggcgttgg tcatgcggcg atcctaccca cgatcgcacc ggcgggtcaa tggacaatgg
      241 agcggaaccc gggcggcgac gcggtgccgg cccggacgac gcggcgttac ggcgcgtcga
      301 tgaatcgatc gcggaggccg ggatccggga cgagacagga ttcccggcgg ccgaaccagc
      361 gataccgatt gcgggcgacc cggtcgtaga ccgcgtcccg gatcggccgc ggcaccgcga
      421 tcagcgcgta caaccagggc caccacccgt ccagataccg cgccgtgcgc aaggccgcgg
      481 cggatcgggt gaagtgtttg cccgcctcca ccagcacgaa cgtcttgaac tcgtcgcccg
      541 gcaacccgaa ccgcgccagg agccgttggc cgatcggcga ttgcatggac gcgaagcgca
      601 cgcgcccgcg gctgtctcgg gctatcacga accggaccga ccccacgcac aggttgcacg
      661 cgccgtcgta taggacgagc gggaagggat actccggctg atccggcgac gacatcggcc
      721 gcggctcgga cacggatggt ctcgtcatca ccgccgcaga ataccacacc ccgccgccgg
      781 acttgggcgc ggcgatgcgg tatactcacg cgcgaatttt cccgcgcacg ggatcgaggg
      841 ccatgaccga cacgcgcacg gttgactgcc tgatcatcgg cggcggaatc gccggaacca
      901 ccgcggccga gggtctcagg aaaaagtccg cggcacgcat catgatcgtc accgaggaac
      961 cgcatccgct gtattcccgg gtgcggctcc ccgactacct ggtggcgcag atcccgcgcg
     1021 agcgcgtgtt catgaaggac gaggcctggt accgcgaacg cggcatcgac ttggtgcggg
     1081 gacgctcggt ccacgccctg tcgacggccg atcgctccgt gcgcctcgac gacggctcga
     1141 cggtgcgcta cggcgcgttg ctgctcgcca tgggcggggc cgcgcgccga ctgtcgtgcg
     1201 agggcgcgga ctggaccggc gtccactatc tgcgcaccat cgacgacgcg gaccggatcc
     1261 ggtcctcgat tcccgcggcc acgcgcgcgg tggtggtggg cggaggattc atcggcctgg
     1321 aactcgcgcg gtgtttcgcc caccacaacc ggccgaccac catggtgctc atggagcccc
     1381 gcttctggcc cgcgttcttc gacgacaccg cgaacgcgtg ggtcgaccgc acgctccgct
     1441 cccgcgggat cgacgtgcgg tactccgagc agctgcaatc cattcgaggc gcgggcgcgg
     1501 tgcgcgacgc cgtgatggcg agcggcaacg cctttccctg cgacatcatg ggcgtgggca
     1561 tcgggatcag caccatgcac ccgttcgtga aggactcggg actcgccatc cgcaagggca
     1621 tcgtgaccga cgaatacttc cggaccaccg acccgcacgt gtttgccgcg ggcgacgtcg
     1681 ccgaattctt cgacctcacc tgcggacgca gcaatcaact cggcaactgg tccaacgcca
     1741 tggaacaagg caaggtcgcg gcgttgaaca tggcgggaga acccacgccc taccgcttcg
     1801 tgggcggcta cgtgatcgcg atcttcggcc tgccggtcgg cctcaccggc gacccgtcca
     1861 cgcctcaggg cagcgaggtc atcctccggg gctcgcccga tcgcggcagc tatgcccgct
     1921 tgatcgtgcg cgacggcgtg atcaaaggcg ccgccgtcct caacgcgccc aaagagaacg
     1981 tcaccgtcgg ccaactcatc
//
LOCUS       DBNGTT010000260         5119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1718304, whole genome shotgun sequence.
ACCESSION   DBNGTT010000260 DBNGTT010000000
VERSION     DBNGTT010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1718304"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..598
                     /locus_tag="AC16BC_05820"
     CDS             200..598
                     /locus_tag="AC16BC_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241172.1"
                     /translation="MKRSITAVVVGYLVLAVAIAAKLLAMKRFVPEVLPAPGEDVFPD
                     TAWLLAMLGSDIGLMIGAGYLTAMIASRTPVIHSVILGAAMVILGLVWMFVFWGWMPL
                     WYQVVLVSIALPAAATGGMLRSPAGGAPTS"
     gene            808..1233
                     /locus_tag="AC16BC_05825"
     CDS             808..1233
                     /locus_tag="AC16BC_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241173.1"
                     /translation="MVRPMVRQLLVSTWLLMLLTPVFAADPPSKPPKMSELERYVRIQ
                     IEIGAAMKEFFSSMGPGAVNPPPEQREAMVEQVDQTVSAIIKKYGLTTEEYNRRKREI
                     FENDEAVETFLKAHPDLKTQWEALPFKGGGGHGRRSYGE"
     gene            complement(1239..2039)
                     /locus_tag="AC16BC_05830"
     CDS             complement(1239..2039)
                     /locus_tag="AC16BC_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR3241174.1"
                     /translation="MSPAVYTAYKIAKLGVYPLTWILVLLLLALVASWRGHWRWLRFC
                     VCSAFLLAYGLSLPPVARVLTKPIEQRYAPPTSHDRVGAEDRFDAVVVLAGGVQRKGG
                     LRREDRLDPGSLDRVVCGRALMAARLAPVVIFSGGSGDPLGALPPAADIMARTYEALG
                     PLSGEVRTEPRSRTTYENAVETRQLLGSGKRIALVTSALHMPRAMALFAQQGLRATAF
                     PCEYLTGPRETGYREYVPDVPYFSQSTEAISEWVGLWLYTLAGKAAKL"
     gene            2236..3798
                     /locus_tag="AC16BC_05835"
     CDS             2236..3798
                     /locus_tag="AC16BC_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3241175.1"
                     /translation="MALPLNSRSVLFRPGDVNAFFGLAVDNLTQMVIMASILTGVFGV
                     PADVVLYGMIPGSVVGVLIGNLVFTRMAVSLAARTNRTDVTAMPLGIDTPSLFAFTFG
                     IVGPAYLATHDGTLTWRIASAVIVTVGVVKIGGAFIGDAVRRVIPRAGLLGPIAAIAL
                     VLIAFFPSLRMMHDPIPAFISLGVILVCLIGRHQFPGRVPGAFAAVMVGLGVYYALQA
                     MGLNAPHGALQDAQNQWRISLPWPSLGFIEGLPSLPTYLPIALPFALVVVIGGIDVTE
                     SASSSGDEYSAGRVILTDGVSTLIGGLCGAVVQTTPYIGHPAYKRMGGGAGYTLAAGL
                     VVGLGGAFGHISFLAGLIPEAVVAPILIFIGLEITAQTFAATPKAHHPALAIAFIPVM
                     ADLVLIQTNALLGNLGVAPAALHGEFAGTYRAVLLLGNGFVVTALIWAGTLAFIIDGR
                     LRDAAALTAVGAVSVLFGVMHSPYDNGRLFLPWLAETSTPWVLCAAYAAVSCWLLLMA
                     ARSGSQAFPNRP"
     gene            complement(3813..4649)
                     /locus_tag="AC16BC_05840"
     CDS             complement(3813..4649)
                     /locus_tag="AC16BC_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3241176.1"
                     /translation="MKARSGRGISIADAAEAAGVSFESLESWESGTGVPSPVQLAAVG
                     RVLGLDSDKLVMLQQNKGHPDPVSVVPRTSGSLRVETLTGFMRGYAVHAYLIYRDGEP
                     DAVLIDTGYEPIRAVEAVLNRRLLLRWVALTHCHHDHMEGAPVLKAQTGARIAVPKAE
                     WASYRSYHREAPDLAVEDGTDVEVSSRLTLRAISTPGHTVGGTCYLTDHVCAVGDALF
                     ASSMGRAMSPDGYRSLREAITNRILTLHSDTVLLPGHGPVTTVHDELRNNPFFPVSQL
                     PG"
     gene            4810..4962
                     /locus_tag="AC16BC_05845"
     CDS             4810..4962
                     /locus_tag="AC16BC_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241177.1"
                     /translation="MKLVNATMIGDTHIVLSEPLPKDAEEITIRIRLATPTPSRPAME
                     LAKQHG"
BASE COUNT          886 a         1689 c         1566 g          978 t
ORIGIN      
        1 ccgggcctgc gtccccgacc gctctcgctg aggtcgcgat ctgatccgcc gagcccgtcg
       61 ttcctccacg cgggtgatat cccatggacg gatcacgcgt tggcgtcttg atcgcgccga
      121 ctcgtacccg gcccgccttg acccgccaca agggatgccg tacgatccgc gcgattgagg
      181 gacaggcgtc acaccaatca tgaagcgaag catcaccgcg gtggtggtcg ggtatctggt
      241 ccttgccgtg gccattgcgg ccaagctcct ggccatgaaa cgcttcgtcc ccgaagtgct
      301 gccggctccg ggcgaggatg tgtttcccga caccgcatgg ctcctcgcca tgctgggttc
      361 cgacattggc ctgatgatcg gagccgggta tctgaccgcg atgatcgcat cgcggactcc
      421 ggtcatccac agcgtgatcc tcggcgcggc catggtcatt ctggggctgg tgtggatgtt
      481 cgtgttttgg ggctggatgc ccctgtggta tcaagtcgtc ctggtgtcga tcgcgctgcc
      541 cgcggccgcg accgggggca tgttacgatc gcccgcaggc ggcgcgccga cgtcctgacg
      601 cgcagtcgcc cgcaccgcct cgaccgggat aagcctcgcg acccacaacg ctgtgccgac
      661 ctcatccagc tcggagagga cctccgccct ccgctatcgt gattgacaca atcagtcgtc
      721 cgcgcctatc atcgcacaac cctttatacg ctcgacgggc cgacttggct cattgctccg
      781 cggctccacg agcagaacag gatgcgcatg gtcaggccga tggtccgaca actgctggtg
      841 agtacgtggt tgctgatgct cctcacgccc gtgttcgcgg cggatccgcc gtcgaaaccg
      901 cccaagatgt ccgagctgga acggtacgtc cggatccaga tcgagatcgg agcggccatg
      961 aaggagttct tttcctcgat gggccccggg gccgtgaatc cgcctccgga acagcgcgag
     1021 gccatggtcg aacaggtcga tcaaacggtg tccgcgatca tcaagaaata cgggctcacg
     1081 accgaggagt acaaccgccg aaagcgggag atttttgaaa acgacgaagc cgtagagacc
     1141 ttcctcaagg ctcaccccga cctcaaaacc cagtgggaag ccttgccgtt taagggcggg
     1201 ggcggccacg gtcgccgctc atacggcgaa taggccacct acagtttcgc cgctttcccc
     1261 gccagggtgt agagccatag gcccacccac tcgctgatcg cctccgtgct ctgcgagaaa
     1321 tacgggacgt ccggaacgta ttctcgatat cccgtttcgc ggggcccggt caaatactcg
     1381 caaggaaacg cggtcgcgcg cagcccctgt tgcgcgaaca aagccatggc gcgtggcatg
     1441 tgcagggcgg acgtgaccag agcaatccgt ttaccggaac cgagcagttg acgggtttcc
     1501 accgcgttct cgtacgtggt tctggagcgc ggttcggtgc ggacttcccc ggacaacggc
     1561 cccagcgctt cgtacgtgcg agccataata tcggctgcag gcgggagcgc gcccagcgga
     1621 tctccgctcc cgcctgaaaa aatcacaacc ggagccagcc gagccgccat cagcgctcgg
     1681 ccacatacca cgcggtcgag tgagcccgga tccagccgat cttcacggcg tagcccgcct
     1741 ttgcgctgca cgccgccagc caacacgacc acggcgtcga aacggtcttc cgccccaact
     1801 cgatcatgcg atgtgggcgg agcatagcgc tgctcgatcg gtttcgtcag tactcgggcc
     1861 accgggggaa gcgagaggcc gtacgcgagc agaaacgcgc tgcatacgca gaaccggagc
     1921 caccgccaat gcccgcgcca cgaggcgacc agggccaaca gcaagaggac caaaatccac
     1981 gtgagcggat agaccccgag tttggcgatt ttatacgcgg tgtagacggc gggcgacatg
     2041 aaggcgacct cggttgataa cgcgtacgct tgtaacagaa ttcgcgcgtg agagaaaccc
     2101 cctcgcggac cctccgcgcc ctggcatccg gcggatactc ggctgcgccg ttacaaattg
     2161 ttgcaggcga tagccgaatt ctgtatactc agcgctccct gtcgcgttgg cgcgcgacgt
     2221 cggactctcg acgcaatggc cctcccgttg aattcccgca gcgtcctgtt tcgccccggc
     2281 gatgtcaacg ccttcttcgg cttggcggtc gacaacctga cccaaatggt gatcatggcg
     2341 tcgatcctca ccggcgtgtt cggcgtgccg gccgacgtgg tcctgtacgg catgatcccc
     2401 gggtcggtcg tcggggtgct gatcgggaac ctcgtgttca cgcgtatggc ggtttcactg
     2461 gcggcgcgca ccaaccgcac cgacgtgacg gccatgccgc tcggcatcga caccccctcg
     2521 ttgtttgcct ttacattcgg catcgtcggg cccgcgtacc tggcgacgca cgacggcacc
     2581 ctcacttggc ggatcgcgag cgccgtgatc gtcacggtcg gcgtcgtcaa gatcggcggg
     2641 gcttttatcg gtgacgcggt gcgccgcgtg atcccgaggg cgggcctgct gggcccgatc
     2701 gccgccattg ccttggtgtt gatcgcgttc ttcccgtcgc ttcgcatgat gcacgacccg
     2761 attccggcgt tcatttccct cggcgtgatt ttggtgtgtt tgatcggccg ccaccagttt
     2821 cccggccgtg tgccgggcgc gtttgccgcg gtcatggtcg gcctgggcgt ctactacgcc
     2881 ctgcaggcga tgggactcaa tgctccgcac ggcgcgctgc aggacgccca gaaccagtgg
     2941 cgcatctccc tgccatggcc ctcgctcggg tttatcgagg ggctgccctc actgccgacc
     3001 tacctcccga tcgcgctgcc gtttgcgctg gtggtggtca tcggcggcat cgacgtaacc
     3061 gagagcgcat cgtcttccgg agacgaatac tcggcgggtc gggtgatcct gaccgacggc
     3121 gtctcgacgt tgatcggcgg gctgtgcgga gccgtggtcc aaaccacccc ctacatcgga
     3181 caccccgctt ataaacgcat gggcggggga gccggctaca cgctggccgc cggcctggtg
     3241 gtcgggctcg ggggcgcgtt cgggcacatc tcgtttctgg ccggcctcat tccggaagcc
     3301 gtggtggcgc ccattctgat tttcatcggc ttggagatca cggcccaaac ctttgccgcc
     3361 actccgaagg cgcaccatcc cgcgctggcg atcgcgttca tcccggtcat ggctgacctg
     3421 gtattgattc agaccaacgc cctgctgggc aatttgggcg tcgcccccgc tgcactccat
     3481 ggagagtttg ccggcaccta ccgcgcggtg ttgctcttgg gaaacgggtt tgtcgttacg
     3541 gcgctgatct gggccgggac cctggcgttc atcatcgacg gccgccttcg cgacgccgcc
     3601 gcgctgacgg ccgtcggagc cgtctcggtc ctgttcggcg tgatgcactc cccgtatgac
     3661 aatggacggc tgttccttcc ctggctggcg gagacatcga cgccatgggt gttgtgcgcg
     3721 gcatacgcgg cggtgtcgtg ctggttactg ttgatggccg cccgttcggg ttcccaagcc
     3781 ttccccaacc gcccatgacc ttccgggtcg gctcaacccg gcaactgcga aacagggaaa
     3841 aaggggttat tgcgcagctc gtcgtggacc gtagtgaccg ggccgtgacc cggaagcaaa
     3901 accgtgtcgg agtgaagggt gaggattcgg ttcgtgatcg cctcccggag cgagcgatat
     3961 ccgtccggag acatggcgcg ccccatggag ctggcaaaca gcgcatcccc cacggcgcag
     4021 acgtggtccg tgagataaca cgtccccccg accgtgtggc ccggtgtgga gatggccctc
     4081 agcgtgagcc gcgaactgac ttcgacgtcg gtcccgtcct ccacggcaag atccggcgct
     4141 tcccggtgat acgagcggta tgatgcccac tccgccttgg gtaccgcgat ccgcgccccg
     4201 gtttgggctt tgagcaccgg tgcgccttcc atgtgatcgt ggtggcaatg ggtgagcgcc
     4261 acccaccgca ggagtaatcg tcggttgagc acggcttcga cggcacggat cggttcgtac
     4321 cccgtgtcga tcagtaccgc gtcgggctcc ccatcgcggt agatcagata cgcgtgcacc
     4381 gcgtagccgc gcataaatcc cgtcagggtc tcgacgcgga gagagccgct ggttcgcggt
     4441 accaccgaca cgggatcggg atgccccttg ttctgctgaa gcatcacgag tttatccgaa
     4501 tcaagcccca agacacgccc gaccgcggct aactggacgg ggctgggcac ccctgttcct
     4561 gattcccagg actccagcga ttcgaacgaa accccggcgg cttcggccgc gtccgcaatg
     4621 gaaatccccc gaccggaccg cgctttcacg agaacgtcaa accactcatc ctctaactct
     4681 tgcataaaac cgtcgactcc aagaaaaatc tggcaaagac tgcttttctt attgacgatt
     4741 tccaggaatt tcgggtatga tttctcggtt taccgctaag aacttccccc aaaaaggagt
     4801 agtgtcggta tgaagctggt taacgccacc atgatcggag acacccacat cgtgctttcg
     4861 gagcccttgc ccaaggacgc cgaagagatc actatcagaa ttcggttggc gacaccaact
     4921 ccatcacgcc cggccatgga gctcgccaag caacacggct aagctcgctc agttcggccg
     4981 ctctcgtcgc tcggaaatcg cgcccgagct cgctactcag tccgctccca atcgcctcac
     5041 cccaggtccg caactgcgat cctcacggtt gccggaggat cgtgaccttt ttcatcacta
     5101 ccggggtaag ggggcggtc
//
LOCUS       DBNGTT010000261         4292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_57904, whole genome shotgun sequence.
ACCESSION   DBNGTT010000261 DBNGTT010000000
VERSION     DBNGTT010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_57904"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="AC16BC_05850"
     CDS             complement(<1..877)
                     /locus_tag="AC16BC_05850"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR3241178.1"
                     /translation="MRSRVIRLIGVLLAGSTTLLASCSTHRQWGPASPDTLAAVRAFL
                     SANDTDAEQLIPALAARPIDHIENALHQAIANPILREAPVGKQPGRRFQVGNRTFHYA
                     LYVPDSYRAERAYPLVICLHGAGFGGETYIERWEPRLKDGFLLACPTIKDAAWWTRDA
                     EALVMAVLAEVSRTYWVDPDRVFLTGMSNGGTGVFVIGLNHADRFAALVPMASALPRG
                     LYPLLENGRQSRFYIIHGARDEVMPVKYSRDLVSYLQRNGFQIVYREHEKEHPLAGGH
                     FFPQEELPDLVEWLSV"
     gene            1247..>4292
                     /locus_tag="AC16BC_05855"
     CDS             1247..>4292
                     /locus_tag="AC16BC_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7948 domain-containing protein"
                     /protein_id="HIR3241179.1"
                     /translation="MPKWTQGILTKDKAMKLNHIRRNRGLTMTALAGVLALVWASIAG
                     AATAGGTQAVRQAADQASVHGAYGQLPLSFIHNNGQVDAKVRFYEKGSGHATYFTTDG
                     VYLSLTKGRSSSADSSLITLRPQGANPNVQIVADHEQAGKVNYLVGRDPGQWQTNVPT
                     YGAVVYQEIYPGIDLTFYGNNRQLEYDFVVKPGADPRNVRLAYEGIEGLRVTETGDLE
                     IALNGGVILQRKPVVYQMINGQRVDVAGAFTVQTPVQQGSRVFAYGFDVAWYDATQPL
                     VIDPTLVYSTYLGGGDSDIGNSVAVDAAGNAYVAGKTQSVNFPTSGPLQAGLNGGSDV
                     FVTKLNPAGTAAVYATYLGGSGADEAAGIAVDAAGNAYVTGRTSSSDFPTAGPLQATS
                     GGGTDAFLVKLAPTGAGLVYATYLGGGGADFGMGVAVDSAGNAYVTGQTQSSDFPTAP
                     TASALQPTLGSANGDGFVAKVNAAGAALVYSTYLGGSGADTGNGIAVDASGNAYVAGE
                     TQSADFPTTSALQPTFGGGTSDAFVTKLNPVGSLIVYSSYLGGSGSDAGHGIAVGSAA
                     SAYVVGATQSPNFPTVNAVQTTPGGLTDAFVARFGTAGAPLLYSTYLGGSNDDVGLGI
                     AVDAASNAYATGHTASGDFPVVSPLQATSGGASDAFVARLNATGTAFGYSTYFGGNGI
                     DEGHGIAVDAAGNAYLTGMTESSNLDMVNPMQGVFAGTQDAFAAKISPLNELTVVKAG
                     NGNGTVTSSPSGITCGATCSAFFGPGALVSLTALPVPPAAFTGWSGACSGVSSCVVTM
                     DASKSVTATFSIPEPTVTRVSPSPVIYGVTPSQSITIYGSSFVVGARITVGGLTGTTV
                     AGFVASAATPFVQVSSGQLAFYWPNTTLPPGSYAVQVANPASVGGGSGSLADAFVVTA
                     PQPTITNLTPATVLYGVTPSQSITIYGSNFAAGARITVGSLTGTTVAGSVASAATPFV
                     YVTRTQLKFYWANTALPAAAYTVQVTNPASAGGLNGSLANGFT"
BASE COUNT          769 a         1270 c         1494 g          759 t
ORIGIN      
        1 gaaccgaaag ccactcgacc agatcgggca attcttcctg cgggaaaaag tgtccgccgg
       61 ccaaggggtg ttccttctcg tgttcgcggt acacgatttg gaacccgttc cgctgaaggt
      121 acgaaaccag gtctcggctg tacttgaccg gcatgacctc gtcgcgcgcg ccgtggatga
      181 tgtagaagcg cgactggcgt ccgttctcca gtaagggata gagcccgcgg ggcagcgccg
      241 aagccatggg taccagcgcg gcgaagcggt ccgcgtggtt caggccgatc acgaacaccc
      301 cggttccgcc gttggacatg ccggtgagaa atacccggtc cggatcaacc cagtaggtcc
      361 gcgacacctc ggccagcacg gccatgacca gggcttccgc atcccgggtc caccaggcgg
      421 cgtccttgat cgtggggcag gcgagtagga agccgtcctt gaggcggggc tcccaccgtt
      481 caatgtacgt ctcgcccccg aacccggctc cgtgcaggca aatcaccaga ggataggcgc
      541 gctcggcgcg ataggaatcc ggaacataca gcgcgtaatg gaaggtgcgg ttgccgacct
      601 ggaacctccg gccgggctgt ttgccgaccg gcgcttcacg caggatcggg ttcgcgatcg
      661 cctggtgcag cgcgttctcg atgtgatcga tcgggcgggc cgccaaggcc gggatcagtt
      721 gttccgcgtc agtgtcgttc gccgacagaa acgctcgcac cgcggccaga gtgtccggtg
      781 atgccgggcc ccactgccgg tgcgtcgagc atgaggcgag gagcgtggtg ctgccagcca
      841 acagaacgcc gatcagacgg atgacccggg accgcatgcg gccacggtac acgcctgccc
      901 gaggaaatgt aaaggtcggg aaccagggtt attcgggttt ctacctgtgg ttggtgtgga
      961 gtcgacctct gagtgaacca gatcgtccgg tccgatggcc tgaacgaggt atcagatgtt
     1021 gaaaaagatc cgcaggtccg tgccgggcga gggatggacc acgacgcgcg aacgtcacga
     1081 tcgtggcggc gtacacccag atgaggagaa gccttcatcg cggtgtttcc gtccacgctc
     1141 ttaacggttc cttaatggtg cgcatgctag agtgcgcgcc gtagatagca caaacggttg
     1201 ctcgaatagg ggggcatagg gaggtggctc gattcgatcg gtctccatgc ccaagtggac
     1261 tcagggaatc ttaaccaagg acaaggccat gaagctcaat cacattcgaa ggaatcgggg
     1321 tctgaccatg acggcgctgg caggggtgtt ggcgctcgtg tgggcatcga tcgcgggcgc
     1381 ggcgacggcc gggggaaccc aggcagtccg gcaggcagcg gaccaggcgt ccgttcacgg
     1441 cgcctacggg cagttaccgc tgtcgttcat tcacaacaac ggtcaagtgg acgcgaaggt
     1501 gaggttttac gaaaagggca gcggacacgc cacgtacttt accacggacg gcgtgtacct
     1561 gtccctgacc aaggggcggt cgtcatccgc ggactcgtcg ttgatcacgt tgcggcccca
     1621 gggcgcgaat ccgaacgtgc agatcgtcgc ggaccacgag caagcgggca aggtcaacta
     1681 tctcgtcggc cgcgatcccg gtcagtggca aaccaacgtg ccgacctacg gcgcggtggt
     1741 gtatcaagag atctaccccg gcatcgacct cacgttttac ggaaacaatc ggcagttgga
     1801 gtacgacttc gtggtcaagc cgggcgctga tcctcgaaac gtccgcctgg cgtatgaagg
     1861 tattgaaggc ttgcgcgtga ccgaaaccgg ggatctggag attgcgctga acggcggcgt
     1921 gattctccag aggaaacccg tggtctacca gatgatcaac ggacaacgag tggacgtggc
     1981 cggagccttc accgtccaga ctccggtcca gcagggatcg cgggtcttcg cgtacggctt
     2041 cgatgtcgcc tggtatgacg cgacccagcc attggtgatc gacccgacgc tggtgtattc
     2101 cacgtatctg ggtggcggcg atagcgacat cggcaacagc gtggccgtgg atgcggcggg
     2161 caacgcgtac gtggcgggca agacccaatc agtgaacttc cccacatctg gtccgctcca
     2221 ggccgggctc aatggaggaa gcgacgtctt tgtgaccaaa ttgaatcccg cgggcacggc
     2281 ggcggtgtat gccacctatc tgggcggtag cggcgcggac gaggcggccg gtattgccgt
     2341 ggacgccgcg ggcaatgcct acgtgacggg tcggaccagc tcctcggact tccccaccgc
     2401 cggtcctctg caggcgacat cgggcggcgg gaccgacgcc ttcctggtca agctcgctcc
     2461 caccggcgcc ggcctggtgt atgccaccta cctcggggga gggggggcgg atttcgggat
     2521 gggagtggcg gtggacagcg ctgggaacgc gtacgtgacg ggtcagaccc agtcatcgga
     2581 tttcccgacc gccccgacgg cgagcgcgtt gcaacccacg ctgggcagcg ccaatggtga
     2641 tggtttcgtc gcgaaggtca atgcagcagg tgcggcgctg gtgtattcga cctatttggg
     2701 cggcagcggc gctgataccg ggaacggcat cgcggttgac gcgtccggca acgcgtacgt
     2761 ggccggcgaa acgcagtccg ctgattttcc gacaacaagt gccctacagc caacgttcgg
     2821 cgggggaacc agcgacgcct ttgtcacgaa actgaatccc gtcgggtcat tgatcgtgta
     2881 ttcgtcgtac ctcggcggaa gcggcagcga cgcggggcac ggcattgccg tgggttccgc
     2941 ggcgagcgcc tacgtggtgg gggcgaccca gtcccccaat ttccccacgg tgaatgcggt
     3001 gcaaaccacg cccggtgggc tgaccgacgc attcgtggcg agattcggga ccgcgggcgc
     3061 gcctctgttg tactccacgt atttgggcgg tagcaacgac gatgtcggtc ttgggattgc
     3121 ggttgatgct gcctccaacg cctatgcgac ggggcatacc gcgtcgggtg atttcccggt
     3181 cgtgagtccg ctccaggcga cgtccggcgg agcgtctgac gccttcgtgg ccagactcaa
     3241 tgccacgggg accgcgttcg gctactcgac gtatttcggc ggcaacggca tcgacgaggg
     3301 gcatggcatt gcggtggatg ccgcggggaa tgcgtatctg accggcatga ccgagtcgtc
     3361 caatctggac atggtgaacc cgatgcaggg cgtgtttgcc ggcacgcagg acgcgtttgc
     3421 ggcgaagatc agtccgctga atgaattgac cgtcgtgaag gccggcaacg gaaacgggac
     3481 cgtgacgtcc tccccgtccg ggatcacttg cggggcgacg tgttcggcgt tcttcggtcc
     3541 cggggcactg gtgagcttga ccgcgttgcc ggtgccgccg gccgccttca cggggtggag
     3601 cggcgcgtgc agcggagtga gttcgtgcgt ggtgaccatg gacgcgagca agtccgtgac
     3661 cgcgacgttc agcattccgg agccgaccgt cacccgagtg agcccgagtc cggtcatcta
     3721 cggggtcacg ccgagccagt cgatcacgat ttacgggtcg agtttcgtgg tgggcgcgcg
     3781 gatcacggtg ggcggcctga cgggcacgac cgtggcgggc tttgtggcga gcgcggcgac
     3841 cccgttcgtg caggtgagca gcggacagtt ggcgttttac tggccgaaca cgacgttgcc
     3901 gcctggatcg tacgccgtgc aggtggcgaa tccggcgagc gtcggaggcg ggtcggggag
     3961 tctcgcggat gcgttcgtgg tgaccgcgcc gcagccgacc atcacgaatc tcacgccggc
     4021 tacggtgctc tacggggtca cgccgagcca gtcgatcacg atttacgggt cgaattttgc
     4081 ggcgggcgcg cggatcacgg tgggcagttt gacgggcacg accgtggccg gcagcgtggc
     4141 gagcgcggcg actccgttcg tgtacgtgac tcggacacaa ctcaagtttt actgggcgaa
     4201 cacggccttg cccgccgccg cgtacaccgt gcaggtgacg aatccggcga gtgcgggcgg
     4261 gctcaacggg agcctggcga acggctttac gg
//
LOCUS       DBNGTT010000262         5080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3123585, whole genome shotgun sequence.
ACCESSION   DBNGTT010000262 DBNGTT010000000
VERSION     DBNGTT010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3123585"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1023)
                     /locus_tag="AC16BC_05860"
     CDS             complement(22..1023)
                     /locus_tag="AC16BC_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2086 family protein"
                     /protein_id="HIR3241180.1"
                     /translation="MKPSRRTTRYPPLDFTRIKTYSTQSRPSKVRVADIARPHRKGRS
                     LARFLDSLPDILAARTLKAVIRDIVAAHRARKPVIVGMGAHPIKVGLNPILIDLMERG
                     VISGLAMNGAGIIHDLELALVGKTSEEVEAGIDDGSFGMARDTAELLNGAIAEGAARG
                     IGMGQAVGDLISRGKFPHKTMSLLATASRLNLPATVHVAIGTDIIHMHPSADGAAIGA
                     TSLEDFKRLTSVVAHLDGGVYLNLGSAVLLPEVFLKAVTIARNLGHRVVNFTTVNMDF
                     TQHYRPVTNVVRRPTQAGGRGYTLTGHHEIMFPLLAAGVIEALGKRIRPSGNRNRSA"
     gene            complement(1076..1654)
                     /locus_tag="AC16BC_05865"
     CDS             complement(1076..1654)
                     /locus_tag="AC16BC_05865"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR3241181.1"
                     /translation="MIEQIRRSFKDSVQVKDAFIARYEDQIAEVAKKIIAAYKKDHKV
                     LLFGNGGSANDASHIAGELVNRFHRDREGLPAIALACDPSIVTSIGNDYDFREIFSRQ
                     VRAFGRKGDVAVAISTSGNSPNVLRGVEAAKPLGMVTIALTGKDGGKLASMVDYAFIV
                     PSNSTPRIQETHITLGHVLCELVEDALFPANR"
     gene            1825..3216
                     /locus_tag="AC16BC_05870"
     CDS             1825..3216
                     /locus_tag="AC16BC_05870"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3241182.1"
                     /translation="MNTYTWWAAVGWGVAASGAVAAGAAACSLAWRFVRRSVLAQRDV
                     RRSAYRSVVDALRAAHEGAAVPDISLGVLADDVFLSCLVDAYDDGRPENRRRLTAYAE
                     RFGYIERLGGLSRSPWARHRMRSARWLGRLAHPDSVADLTRMTRDRSLRVRSTAVTAL
                     GAVQDPRALTALVALLDGQSFTVGPRRVPASCLTDALAAHGADAVLSVLPRLQSPSPA
                     VRATAADVLARTPHPDPRVRAALAAALEDPDGEVRARAAKAIGRSGDAGAAAPLVKAL
                     SDRVWFVRLQAARAIGVLAYGRAVRPLVSVLTDEWWQVRAAAAAALRHVGDAAVPALT
                     ECLFESRDRYAKEQVVEELQRTRLLLEQVEALDGAHAGAAFVAQRFLREMARHGATAV
                     LLDALRRHPRASVRRRLAAALGTVDVPRVLTVLHDLAEHDRDAGVREAARRALTGRPP
                     GAAGGTAARERAA"
     gene            3213..4598
                     /locus_tag="AC16BC_05875"
     CDS             3213..4598
                     /locus_tag="AC16BC_05875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3241183.1"
                     /translation="MIDAMVSAAARLFAGYAILTGLGGAAVVLSAAWVVIRSGRDDGL
                     PRREAAEDERAVPPVSVVVVAADEDSDIGDRLDALAALDYPAFEIVVVSDGPISPALA
                     RAMARLQTQAVDLIYRPLIKTGRVTGCYHSPITSNLVVLEKEPASTADALNAGLNVAR
                     SPYVCLVESRTWLDRSALRRLMAPVIEDPDRVVVSAGMARVMNGCVVRDGDVAVGACP
                     SNPAVAWAVLGGLRSAVLAAGASPLLGAGAAPRGVLLVHRTEALRVGGVPSDGTIHDL
                     AWLPWTRSGLRSAYVPLPVSWTRVPATFADLARAYRRRVAAAVGAMRGLWNRERRRGE
                     HGAVKHGARFMRSAWTLSALLDPAALAVATLGFGRGDIDFPLLLAVWVSVLVGRTAVS
                     SVAILLHDVTPKRYPSSWDVARLFLAAVFDLTVGRLLALWWTVSTLSCFRRAGPPTVV
                     TPLTAGADTRS"
     gene            complement(4608..>5080)
                     /locus_tag="AC16BC_05880"
     CDS             complement(4608..>5080)
                     /locus_tag="AC16BC_05880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3241184.1"
                     /translation="SIGAFWAVGVAVEVLFMFWSARVMSRISAETMFALAFAGAVARW
                     GMMSAAPGITGLVVAQSLHALTFGAFHIAAVTLIHRTAPPSLRATGQTLYSSLAYGVG
                     SGVSSLFNGALYARWGASALFGVSAVAAAAGLLLAARHLAVTVRSGRTNPTSDE"
BASE COUNT          664 a         1662 c         1894 g          860 t
ORIGIN      
        1 aagggggagg tcttggggct attaggcgct gcgattccgg ttgcctgaag gcctgatccg
       61 cttcccgagc gcctcaatca ccccggcggc cagcagcgga aacatgatct cgtggtgacc
      121 ggtcagcgtg tacccgcggc cgccggcttg ggtggggcgt cgcaccacgt tggtcacggg
      181 gcggtagtgc tgggtaaagt ccatgttcac ggtggtgaag ttgaccacgc ggtgcccgag
      241 attccgggcg atcgtgaccg ctttgaggaa cacctccggc aacaggaccg ctgatcccag
      301 gttcaggtac acgccgccgt cgaggtgggc gaccaccgag gtcaggcgct tgaagtcttc
      361 cagcgaggtg gcgccgatcg cggcgccgtc cgcggagggg tgcatgtgga tgatgtcggt
      421 gccgatcgcc acgtggaccg ttgccgggag gttgaggcgg ctggcggtgg ccaggaggct
      481 catggtcttg tgcgggaact tgccgcggct gatcaggtcg cccacggcct gacccatgcc
      541 gataccgcgc gcggctccct ccgcgatggc gccgttgagc aactcggcgg tgtcgcgcgc
      601 catgccgaag ctgccgtcat cgatgcccgc ttcgacctct tcggaggtct tgcccaccaa
      661 ggccaattcc aggtcgtgga tgatcccggc gccgttcatg gccaggccgg agatgacgcc
      721 gcgctccatc aaatcgatca gaatcgggtt cagcccgacc ttgatcgggt gcgcgcccat
      781 gcccacgatc acgggtttgc gggcacggtg cgcggcgacg atgtcgcgga tcacggcttt
      841 gagcgtgcgg gccgccagga tgtcgggcag gctgtcgaga aagcgcgcca ggctccggcc
      901 cttgcgatgc ggcctggcaa tgtcggccac gcggactttg ctcggccgcg attgcgtgga
      961 gtaggtcttg atccgcgtaa agtcgagcgg ggggtagcgg gtcgtccggc gggagggttt
     1021 cactgcgggg cggtcgaact cgaagcgcgc gcgccgggct ccggcggtgt cgggatcacc
     1081 gattcgcggg aaagagcgcg tcttccacca gctcgcacag cacgtgtccg agcgtgatgt
     1141 gggtttcttg gatgcgcggg gtgctgttgg acggcacgat gaacgcgtag tcgaccatgg
     1201 acgcgagttt gccgccgtcc ttgccggtca aggcgatggt caccatcccc aacggcttgg
     1261 ccgcttccac gccgcgcagc acgttcgggg agttgccgct ggtgctgatg gcgacggcta
     1321 cgtcgccctt ccggccgaac gcgcgcacct ggcgggagaa gatttcccgg aagtcgtagt
     1381 cgttgccgat gctggtcacg atggacgggt cgcacgccag cgcaatggcg ggcaggcctt
     1441 cgcggtcgcg gtgaaaccgg ttgaccagct ctcccgcgat gtgcgacgcg tcgttcgcgc
     1501 tgccgccgtt gccgaagagg agcaccttgt ggtctttttt ataggcagcg atgatcttct
     1561 tcgccacctc ggcgatttgg tcctcgtacc gggcgataaa cgcgtccttg acctggacgc
     1621 tgtctttgaa cgaccgacgg atctgctcaa tcatggtgag ttccgaatcg cctgcgcggc
     1681 cttgagtcgc ttccgaaaag gggaataccc cgcggcgaca tcttatgtcc cgttcttccg
     1741 ggttgtcaag gtgcgcgggg gcgggtatga tctgtctgcg ttgcggtcag ggagggcccg
     1801 caacgtttcc gaatgacaca cgtcgtgaat acgtacactt ggtgggcggc cgtgggctgg
     1861 ggcgtggccg catccggcgc ggtggcggcc ggggcggcgg cgtgttcgct ggcctggcgg
     1921 ttcgttcgcc gctccgtgct cgctcagcgc gatgtccggc gctcggcgta ccgctcggtc
     1981 gtcgacgcac tgcgcgcggc gcacgagggc gcggccgtgc ccgacatctc gctgggcgtc
     2041 ttggccgacg atgtgttcct gtcctgtttg gttgacgcgt atgacgacgg gcgcccggag
     2101 aaccgtcgtc gtctgaccgc atacgccgaa cgcttcggat acatcgaacg attgggcggc
     2161 ctgagtcgga gtccctgggc gcggcaccgc atgcgctccg cgcggtggct cggtcgcttg
     2221 gcgcatcccg acagcgtggc ggacctgacc cgaatgacgc gggatcgctc gctgcgcgtg
     2281 cgttcgacgg cggtgaccgc gctgggcgcg gttcaagacc cgcgcgcgtt gaccgcgctg
     2341 gtcgcgctcc tggacggtca gtcgttcacc gtcgggccgc ggcgggtgcc cgcgtcctgc
     2401 ctgaccgacg ccctggcggc tcacggagcc gatgccgtct tgtccgtctt gccccgtttg
     2461 cagagcccgt ccccggcggt tcgcgcgacg gccgccgacg tgctggcgcg cacgccgcac
     2521 cccgatcccc gcgtgcgcgc ggcgctggcg gcggcgttgg aggatccgga cggcgaagtg
     2581 cgggcgcggg cggccaaagc catcgggcgg agcggcgacg cgggagcggc ggcgccgttg
     2641 gtcaaggcgt tgtcggaccg ggtctggttc gtgaggcttc aggccgcgcg cgcgatcggg
     2701 gtcttggcgt acggccgcgc ggtgcgcccg ctcgtgtccg tcctgaccga cgagtggtgg
     2761 caggtgcgcg cggccgccgc cgccgcgctc cgtcacgtgg gcgacgcggc cgtgccggcg
     2821 ctcaccgagt gtctgttcga gagtcgggat cggtacgcca aagagcaggt ggtggaagaa
     2881 ctgcagcgca cgcggttgtt gctcgaacaa gtggaggcgc tggacggcgc gcacgccggc
     2941 gcggcgttcg tggcgcagcg gttcctgcgc gagatggccc ggcacggcgc gaccgcggtg
     3001 ttgctggacg cgctgcggcg tcacccgcgc gcgagcgtga ggcggcggct ggcggccgcg
     3061 ctcgggaccg tggacgttcc ccgcgtgctc accgtattgc acgacttggc cgaacacgac
     3121 cgcgacgccg gcgtccggga ggcggcgcgt cgcgcgctca ccggacgacc gccaggggcc
     3181 gcgggaggga ccgcggcgcg ggagcgcgcc gcgtgatcga cgcgatggtg tcggccgcgg
     3241 ctcggttgtt cgcgggctac gccatactca ccgggctcgg gggagcggcc gtggtcctgt
     3301 cggcggcgtg ggtcgtcatt cggtccgggc gcgacgacgg attgccgcgg cgggaagccg
     3361 cggaggacga acgcgcggtg ccgccggtgt cggtggtcgt ggtggccgcc gacgaggact
     3421 cggacatcgg cgaccgcctc gacgccttgg ccgcgctgga ctatccggcg ttcgagatcg
     3481 tggtggtgtc cgacggcccc atcagtcccg cgctcgcgcg ggccatggcg cggttgcaga
     3541 cgcaggccgt cgatttgatc taccggccgt tgatcaagac cggccgcgtc acgggctgtt
     3601 accacagccc gatcacgtcc aacttggtgg tgctcgaaaa ggagccggcc tcgaccgcgg
     3661 acgcgctgaa cgccggcctc aacgtcgccc gcagcccgta tgtctgcctg gtcgagtctc
     3721 ggacctggct ggatcgctcg gcgttgcggc gactcatggc gccggtgatc gaagaccccg
     3781 atcgagtggt ggtgtccgcc gggatggcgc gcgtgatgaa cgggtgcgtc gtgcgcgacg
     3841 gcgacgtggc ggtcggcgcg tgtccgtcca acccggccgt ggcctgggcg gtgctcggcg
     3901 gccttcgcag cgcggtactt gccgccggcg cgagcccctt gctgggcgcc ggggcggcgc
     3961 cgcgcggcgt actgttggtg catcggaccg aagcccttcg agtcggcggc gtgccctccg
     4021 acggcacgat ccacgatctg gcgtggttgc cctggacgcg atccgggctg cgctcggcct
     4081 acgtgccgct tccggtgtcc tggacccgcg tgccggcgac gttcgccgac ctggcgcgcg
     4141 cgtatcgtcg gcgcgtcgcg gccgcggtcg gcgcgatgcg gggactgtgg aatcgggagc
     4201 ggcgccgggg ggaacacggc gcggtcaaac acggcgcccg gttcatgagg agcgcgtgga
     4261 cgctgagtgc gctgctcgat ccggcggcgc tggccgtcgc gacgctgggg ttcggtcgcg
     4321 gagacatcga ttttccgctg ttgctcgcgg tatgggtcag cgtgttggtc ggtcgaaccg
     4381 ccgtgtcatc ggtcgcgatc ctgctgcacg acgtgacccc gaagcggtac ccttcctcgt
     4441 gggacgtggc gcgattgttc ctggccgcgg tgttcgatct gacggtggga cggttgctcg
     4501 cgttgtggtg gacggtctcg acgctgtcgt gcttccgccg ggccggtccg ccgacggtcg
     4561 tcacacccct caccgcgggc gcggatacgc gctcgtaacc gcgttcgtta ctcgtcgctc
     4621 gtcgggttcg tccgaccgga cctgaccgtg accgccagat gtcgcgcggc caggaggagt
     4681 ccggccgcgg ccgcgaccgc gctgaccccg aacaacgccg acgcgcccca ccgcgcgtac
     4741 aatgcgccgt tgaagaggga actgaccccc gatcccacgc cgtaagccag actgctgtag
     4801 agcgtctgcc ccgtggcgcg caacgacggc ggcgcggtgc gatggatcag cgtgacggcc
     4861 gcgatgtgaa acgcgccgaa cgtgagcgcg tggagcgatt gcgccacgac cagtcccgtg
     4921 atccccggcg cggcgctcat catcccccag cgagccaccg cgccggcgaa cgccagcgcg
     4981 aacatggttt cggcgctgat ccgcgacatc acgcgcgccg accaaaacat gaagagcacc
     5041 tcgacggcca cccccacggc ccagaacgcg ccgatcgacg
//
LOCUS       DBNGTT010000263         2554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1079558, whole genome shotgun sequence.
ACCESSION   DBNGTT010000263 DBNGTT010000000
VERSION     DBNGTT010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1079558"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="AC16BC_05885"
     CDS             <1..181
                     /locus_tag="AC16BC_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3241185.1"
                     /translation="RQAREVMTVQPKTIQSSVLAVEALATMERFAITALVVVDADGRV
                     AGVVHLHDLLKAGIA"
     gene            192..707
                     /locus_tag="AC16BC_05890"
     CDS             192..707
                     /locus_tag="AC16BC_05890"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR3241186.1"
                     /translation="MSAWSRGLSAKANAVSFLFLDVDGVLTDGLVTFTSEGHEIQSFH
                     IHDGHGIKQLTRAGIDVGILSGRRSPIVDRRAKELGLVEVHQGAEDKLLVYEAFLARH
                     GLSDRQIAYMGDDVPDVPILKRVGLALSVRNAHDAAKRSADYVTTRRGGEGAVREVCD
                     LLLRARGIRLP"
     gene            704..1690
                     /locus_tag="AC16BC_05895"
     CDS             704..1690
                     /locus_tag="AC16BC_05895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3241187.1"
                     /translation="MSAGQRFIRTLIVVVLDVVSAAAALIPWRLGVAIGGTIGRVAWY
                     ALPSTRRTALDNLAMAFPDLSEQERARIGRASLANLGRAAMELLMLWRRPGTPVERWC
                     AFDDEDRLRDALAPGRGAIFVTGHTGNWELLAAVVARRGYPLSVVATPVYDDRIDQRL
                     VGARAVHGIETIRRGSAAAGRQLLSALRRNAVLGMLIDQDTDVDGVFVPFFGRLAYTP
                     AGAAALALRTDARVVCGFLVREGASRHRVVLEGPIDLVRTGETDQDVLENTRRFTDLI
                     ERHISTYPDQWIWFHRRWKRRPKSATTHGAVASPEPPVEPASPAASANLLRA"
     gene            1687..2256
                     /gene="pgsA"
                     /locus_tag="AC16BC_05900"
     CDS             1687..2256
                     /gene="pgsA"
                     /locus_tag="AC16BC_05900"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR3241188.1"
                     /translation="MNPRGHQLNLPNLLTLTRLGLIPVFVVLFLRESSPMSALVAAAV
                     FGLASITDWLDGYVARSRQQITDLGKLLDPVADKVLVLAALVVLVEANRAPGWLVIIL
                     LAREFAVTALRAVAAIDGQVIPADRLGKWKMVAQTAAVLVLILEPALPSWAHRGGLSL
                     LVAATVMALVSGWQYAAAYRRSLGPTSAT"
     gene            2253..>2554
                     /locus_tag="AC16BC_05905"
     CDS             2253..>2554
                     /locus_tag="AC16BC_05905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3241189.1"
                     /translation="MTADSSLLVGLAIVLFAYMIGTIPFGVVVTRWIGASDPRTKGSG
                     NIGSTNVLRVAGKGPAAATLLLDAGKGAVAVWAASLLTADHTARWAEISGMAVVAG"
BASE COUNT          379 a          829 c          881 g          465 t
ORIGIN      
        1 acgccaggcc cgcgaggtga tgacggtcca acccaagacc atccagtcct cggtgttggc
       61 cgtcgaggcg ctggcgacca tggagcgttt cgccatcacc gcgttggtgg tcgtggacgc
      121 ggacggccgc gtcgccggcg tcgtccatct gcacgacctc ttgaaggccg gcatcgcctg
      181 atcagccacc gatgagcgcc tggtcgcgcg gcctctccgc caaagccaac gccgtgtcgt
      241 ttctcttcct ggacgtggac ggcgtgttga ccgatggact cgtcaccttt accagcgagg
      301 gacacgagat ccagtcgttc catatccacg acggccacgg catcaagcaa ctgacgagag
      361 ccggcattga cgtcggcatt ctgagcggtc gtcgatcgcc gatcgtggac cggcgggcga
      421 aggaactcgg cttggtcgaa gttcaccagg gcgcggagga taagctgctc gtctacgagg
      481 ccttcctggc gcgccacggc ctttcggacc ggcagatcgc ctacatggga gacgacgtcc
      541 cggacgtgcc gattctcaaa cgcgtagggt tggccttgtc cgttcgcaac gcgcacgacg
      601 cggccaagcg gtcggccgac tacgtgacca cgcgacgggg cggggagggc gcggtccggg
      661 aggtgtgcga tctgctgctg cgagcgcggg gaatacggct gccgtgagcg ccggacaacg
      721 gttcatcaga accctcatcg tggtcgtcct cgatgtcgtc tccgcggcgg ccgcgttgat
      781 tccctggcgg ctcggcgtgg ccatcggagg gacgattggc cgcgtcgcct ggtacgcgct
      841 gccgtccacg cgtcgaaccg cgctcgacaa cctggccatg gcgtttcccg atctctccga
      901 acaagagcgc gcccgcatcg gccgcgcctc gctcgccaac ttggggcgag ccgccatgga
      961 actgttgatg ttgtggcgtc gtcccggcac gcccgtcgaa cgatggtgtg cgtttgacga
     1021 cgaagaccgg ctccgagacg cgctcgcgcc gggacggggg gcgatttttg tgaccggcca
     1081 taccggaaac tgggaacttc tggccgccgt cgtggcgcgt cgcgggtacc cgctgtcggt
     1141 cgtggccacg ccggtctatg acgaccgcat cgatcaacgg ctcgtcggag cccgcgcggt
     1201 ccacgggatc gagacgatcc gacgcggcag cgcggcggcg ggtcggcagc tcttgtccgc
     1261 gctgcgccgc aacgccgtct tgggaatgct gatcgaccag gacacggacg tggacggcgt
     1321 gttcgttccg tttttcgggc gcctcgcgta tacgccggcc ggagccgccg cgttggcgct
     1381 gcgaacggac gcgcgcgtcg tgtgcggttt cctggtccgt gaaggggcct cgcggcaccg
     1441 ggtggtcttg gaagggccga tcgaccttgt gcgaaccggc gaaaccgacc aggacgtgct
     1501 ggagaacacg aggcgtttca ccgacctgat cgaacgacac atcagtacgt atccggacca
     1561 atggatttgg tttcatcgac ggtggaagcg ccgtcccaag tcggcgacga cgcacggcgc
     1621 cgtcgcgtcg ccggaaccgc cggtggagcc cgcgtccccc gcggcctccg caaatctact
     1681 tcgggcatga acccgcgcgg ccatcaactc aacctcccca atctcctgac gctcacgcga
     1741 ctcggattga tccccgtctt cgtggtgctc tttctgcgcg agtcctcgcc catgagcgct
     1801 ctggtcgcgg ccgcggtgtt cggccttgcg tcgatcaccg actggctgga cggatacgtg
     1861 gcgcggagcc gccaacagat caccgacctc gggaaattgc tggatccggt cgcggacaag
     1921 gtcctggtcc tggcggccct cgtggtcttg gtggaggcca accgggctcc gggatggttg
     1981 gtgatcattc tgcttgcccg agagttcgcg gtcaccgcgt tgcgggccgt cgccgccatc
     2041 gacggacagg tgatcccggc cgatcgcctc ggcaaatgga agatggtcgc gcagaccgcc
     2101 gcggtactcg tgctcatttt ggagcccgcg ctgccgtcct gggcccatcg cggcggactg
     2161 agcctccttg tcgcggccac cgtgatggcc ctggtctcgg gctggcaata tgccgcggcc
     2221 tatcgccgct cgctcggccc gacttccgcg acatgaccgc ggattcgtcc ctcctggtcg
     2281 gcttggcgat tgtgctgttc gcgtatatga ttggcacgat tccttttggc gtggtggtca
     2341 ctcggtggat cggcgcctcg gatcccagga ccaagggaag cgggaacatc gggagcacga
     2401 acgtcctacg ggtcgcgggc aaggggcctg cggccgccac gttgttactg gatgcgggca
     2461 aaggggccgt tgcggtgtgg gcggcgtcgt tgctgacggc ggatcacacg gcgcggtggg
     2521 cagagatttc cggcatggcg gttgtcgcgg gtca
//
LOCUS       DBNGTT010000264         4940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3507579, whole genome shotgun sequence.
ACCESSION   DBNGTT010000264 DBNGTT010000000
VERSION     DBNGTT010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3507579"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="AC16BC_05910"
     CDS             complement(<1..693)
                     /locus_tag="AC16BC_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241190.1"
                     /translation="MNNLCAITVITFLEGLRSRVFFGLFLLALALFSSTYVLSYLFPR
                     DVVKVAVDLGLGITSLIGLVLTLFLGTQLLAKDLEKRTIHTVLAKAVSRSEYVVGKFA
                     GLSLIIVCAMAMLGVFAGSAAWAVDLLTSDQYGVMHWPAFVVSLGAMTLMLVLLISVI
                     IFFSSFTSSTFVALGLTLVVYFIGQSTEALKSFLASGAEGMTINPMFGNVVALAYYVF
                     PNLAVFDFKTYAA"
     gene            complement(690..1619)
                     /locus_tag="AC16BC_05915"
     CDS             complement(690..1619)
                     /locus_tag="AC16BC_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3241191.1"
                     /translation="MSSIAFDNVTKVYGGSRSPRVAVSGLSLTVPSGETFGFLGPNGA
                     GKSTAIKVLLNLIFPTSGRALLLDTPVHDPIVRRRVGYLPENPTFYDYLTPEELLWFG
                     GKASGMATTHIKERTAKLLDLVDLARVRRQRLRTFSKGMLQRAGVALALINDPEIIIF
                     DEPMSGLDPLGRKLMADLIRGLKADGKTVFFSSHILRDIEDLCDRVGIIVGGRLRRVA
                     TLAELASAESGGWRVVLRGTGEPIRAHLAGLACTCSERAGLTEIQTPERDIVKLLDRL
                     GGLQEAIVSAAPLRPTLEEILLREIERAGAEPS"
     gene            complement(1826..2473)
                     /locus_tag="AC16BC_05920"
     CDS             complement(1826..2473)
                     /locus_tag="AC16BC_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3241192.1"
                     /translation="MFKSMQQLKAKASKGERGFTLVELLIVVAIIGILAAIAIPQFAA
                     YRMRAFNSSAGSDTRNTKTAEEALFVDFQRYGLTAGGATLAAAPGGFGAGALLTGPLG
                     PATTAVAGAMLTVTTAALANVGTGIGIGNLVTLQSSTDAAGASYIVAARHQNGDTAFA
                     TDSDGTAMYQVRNSAWVGVPLAISATVPAPPTAGADDIALTPAGGGAPVANWAAL"
     gene            complement(2723..4429)
                     /locus_tag="AC16BC_05925"
     CDS             complement(2723..4429)
                     /locus_tag="AC16BC_05925"
                     /inference="COORDINATES: protein motif:HMM:NF051348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241193.1"
                     /translation="MAPAVAYSHFIEGYLRELQGEDASAISEFEQALQVDPGSVTLRH
                     HVAALAFRLGRYEEGVSQGEEILRHDPNHLPTIFLLARWYAASGTQVDRAVELFERMA
                     KLEPDKPEPYLNLGLLYARLQRYEDAERTIRRVLAKEPNSPTALLYLGKIAAAQQHWR
                     DAETNLERAIEAAPYSEAAYIALGDLYIQQGDRTRAVKVYKRLLERVDPRDAEAVGRL
                     VHLYLQDRTYDQALALLDDLLKADPKNAEAHLLKGRIYGEIGKPEDAVREMEVVIAIR
                     PNDTAAIYYLGRLYDEQKQPDKAIAQFERLVAMNADVLEAYLQLGILYTRAKRLDEAE
                     AVVEKVKAKASDRPEVYLVLGFVYSQREDYARAASVLEEGVTIQPENATLHFNLGLAY
                     DKLKQFDRFVQELEEAIRLDPKYAEALNYLGYTYAEKEIKLPEALDLINRALTIKPDD
                     GAYVDSLGWVHFKLGQWDDAVKDLERAVSLLPDDAVVHEHLGEAYLKKSRRKDARESW
                     MKALELDPMNAKLIDRFKGAGFGDPEAEERFQKAKTKKLTEDPATAAPAPQDPEQRQS
                     GI"
     gene            complement(4575..4772)
                     /gene="tatA"
                     /locus_tag="AC16BC_05930"
     CDS             complement(4575..4772)
                     /gene="tatA"
                     /locus_tag="AC16BC_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR3241194.1"
                     /translation="MFGLGMPELLVILVLVLLIFGASRLPEIGAGLGKAIRGFKKGVQ
                     DPTDLGASSKSDPSQPGKPSA"
BASE COUNT         1010 a         1575 c         1438 g          917 t
ORIGIN      
        1 cgccgcatag gttttgaaat cgaagaccgc gagattgggg aacacatagt acgcgagcgc
       61 caccacgttc ccgaacatgg gattaatcgt cattccctcg gccccagagg cgagaaaact
      121 cttaagcgcc tcggtggatt ggccgatgaa gtacacaacc agggtcagcc ctaacgcgac
      181 aaacgtgctt gacgtaaacg acgagaaaaa gatgatcacc gagatgagga gcacgagcat
      241 caacgtcatt gcgcccaggc tcacgacaaa ggctggccag tgcatcacgc cgtactggtc
      301 cgaagtcaga aggtctacgg cccaggcggc tgatccggcg aacacaccca gcatagccat
      361 ggcacagaca atgatcagcg agaggcccgc gaacttgccg accacgtatt cggaccggga
      421 gactgcctta gccaacaccg tgtggatggt gcgtttctcc agatccttgg cgaggagttg
      481 cgtcccgaga aagagcgtca atactagacc gatgagcgag gtaatgccga gccccaggtc
      541 caccgcaacc ttgaccacgt cgcgcggaaa caggtagctg agcacgtagg tcgaggaaaa
      601 gagtgcaagc gccagcagaa agagcccaaa gaacacccga ctgcggagcc cctccagaaa
      661 cgtgatgacc gtgatcgcgc agagattgtt catgacggct cggctccagc ccgctcgatc
      721 tcccgcaaca ggatctcctc cagggttggt cgaagcggcg cagcggagac gatcgcctcc
      781 tgcaggcctc ccagtcggtc gaggagcttc acgatgtccc gctccggcgt ctggatttcg
      841 gtcaaccccg cccgctccga gcacgtgcac gccaagcccg ccaagtgtgc ccggatgggc
      901 tctccggttc cgcgcagtac cacccgccag ccacccgact cagccgacgc caattcggcg
      961 agtgtggcca cgcgccgcag ccggcccccg acgatgatcc cgacgcggtc gcagagatcc
     1021 tcgatgtcgc gcaaaatgtg ggagctaaag aagaccgtct tgccgtcggc cttgagcccg
     1081 cgaatcaagt cggccatgag cttacggccc aacggatcaa gaccagacat cggttcatcg
     1141 aagatgataa tttccggatc attgatcaga gcaagcgcca cgcccgcacg ctggagcatt
     1201 cctttggaga acgtccgcag gcgctggcgc ctcacccgcg cgagatcgac gagatccagc
     1261 agtttcgccg tccgctcctt gatgtgcgtc gtggccatcc ccgacgcttt gccgccgaac
     1321 cacagcaact cctctggcgt gagataatcg taaaacgtcg gattctcggg caaatagcca
     1381 acacgccggc ggacaatggg gtcgtggacc ggcgtgtcca gaagaagggc ccgacccgac
     1441 gtggggaaaa ttaggttcag tagcaccttg atcgccgtgc tcttgcccgc cccgttggga
     1501 ccaaggaacc cgaaggtctc ccccgacggg acggtcaggc tcaagccact caccgcgacg
     1561 cgcggcgagc gtgacccacc atagaccttg gtcacattgt cgaaggcgat gctgctcacg
     1621 aaatcctctc gcgtctgccg cgtagactat agcagtcggc tattcacggt caacagaacc
     1681 ggagtcgact gtgcagaaaa accccctcac aggcgaacac gtcccgccgg gggacacacc
     1741 tgtcagacgt tgcgggacaa ataaggaggg gggccgggaa ttcccggccc cccagttcgt
     1801 ccttatttcc ggtgagcgtg tgagcttaca gagccgccca gttcgccacc ggcgctccgc
     1861 cgcccgcagg ggtgagcgca atatcatcgg caccagcggt cggcggagcc ggcaccgtcg
     1921 ccgaaatcgc aagtggtacc cccacccagg ctgagttacg gacctgatac atcgcggttc
     1981 catccgaatc cgtcgcaaat gcggtatcac cgttttggtg ccgcgcggcc acgatatacg
     2041 acgcgccagc agcgtcagtc gaactctgca gggtcaccag gtttccaata ccaatcccag
     2101 tcccgacatt cgccagcgca gccgttgtca cggtcagcat cgcgccagcg accgcggttg
     2161 tggccggacc gagcggacca gtcagcaagg ccccggcacc gaatccacct ggagcagctg
     2221 ccaacgtcgc gccaccggcg gtaagcccat agcgttggaa gtctacaaac aaggcttctt
     2281 ccgcggtctt cgtattccgg gtatcagacc ccgcgcttga gttaaacgcc cgcattctgt
     2341 acgccgcgaa ctgcgggatc gcgatcgcgg ccaagatacc gatgatcgcg accacgatga
     2401 gcaactcgac cagggtgaaa ccccgctcgc ctttgctcgc ctttgctttg agctgctgca
     2461 ttgacttgaa catgtgtgct cctccccctg gttgatattg gccggcgacc tgtccgccgc
     2521 cctcggctag atctgctaag caggacccat gccagtcgaa cagagggcca gtttgccagg
     2581 aaaaaccggt tcgtcaagcc acttcgaccg aaagtgtgcg tgaacggacg ttccggctgt
     2641 caacagttgt tcatgcgatg acaatttttt gctcagagtc gcggttcggt tcagctgcga
     2701 ccattgcaaa gcggcacggt ggctagatgc cagattgccg ttgctcgggg tcttgaggcg
     2761 caggagccgc tgttgccggg tcttcggtca atttcttcgt cttcgccttc tggaaccgct
     2821 cctcggcctc cggatcgccg aatccggctc ctttaaaacg atcgatgagt ttcgcgttca
     2881 tgggatccaa ttcaagcgcc ttcatccacg attctcgagc gtccttccgc cgacttttct
     2941 tcaggtacgc ctcgccaagg tgttcgtgca ccaccgcgtc atcgggcagc aacgagaccg
     3001 cgcgctccaa atccttgacg gcgtcgtccc attgcccgag tttaaaatgg acccatccaa
     3061 ggctgtcgac gtatgcaccg tcatccggct tgatcgtgag tgcacgattg atcaggtcca
     3121 gcgcttcggg cagcttgatc tctttttcgg cataggtgta gccgaggtag ttcaaggcct
     3181 ccgcgtattt ggggtcgagc cgaatggcct cctccaactc ttggacgaac cggtcgaact
     3241 gtttgagctt gtcgtaggcc aaacccaagt tgaaatggag cgtggcgttc tcgggttgga
     3301 tcgtcacgcc ctcctccagg acagaggcgg cgcgagcata gtcctcccgc tgggaataga
     3361 cgaaccccaa aaccaggtag acttcgggac gatccgacgc cttggccttg accttctcaa
     3421 caacggcctc ggcctcgtcc aatcgtttgg ccctcgtata cagaatgccc aactggagat
     3481 acgcctccag cacgtcggcg ttcatggcca ccaagcgctc aaactgcgcg atcgccttat
     3541 caggctgctt ctgctcgtca tacaagcggc ccagatagta gatcgccgcg gtatcgtttg
     3601 ggcgaatcgc gatgaccacc tccatctccc tcaccgcgtc ctccggtttc ccgatttctc
     3661 cgtagatacg gcccttgagc aggtgggctt cggcgttttt cggatcggcc ttcaggaggt
     3721 cgtccagcag ggccaaggcc tggtcgtacg tccggtcttg gaggtacagg tgcacgagtc
     3781 gaccgacggc ttccgcgtcg cgtggatcga cccgttcgag caaccgttta taaaccttga
     3841 ccgcgcgcgt gcgatcaccc tgctggatat agagatcgcc cagcgcgata tacgccgctt
     3901 ctgaatacgg ggccgcctcg atcgcccgtt ccagattcgt ctccgcgtcg cgccaatgtt
     3961 gctgcgcggc cgcgattttc ccgagataca gcagggcggt cggcgagttc ggctccttcg
     4021 ccagcacccg acgaatcgtg cgttcggcgt cctcgtatcg ttggagccgc gcgtagagca
     4081 atccgagatt gagatacggt tcgggtttgt ccggctccaa cttggccatc cgctcgaaga
     4141 gttccaccgc gcggtcgacc tgggtcccgc tggcagcgta ccagcgagcc agcaaaaaaa
     4201 tcgtgggaag gtggttgggg tcgtggcgca ggatttcttc cccttgggaa accccctcct
     4261 cgtaccgtcc cagccggaac gccagagccg ccacgtgatg ccggagcgtg accgacccgg
     4321 gatcgacctg cagggcctgc tcaaactcgg agatcgcgga ggcatcttcg ccttgcaact
     4381 cgcggagata cccttcgatg aaatgggaat acgccacggc cggtgccacg cgcgtcggcc
     4441 ccggcggagg caacgcgggg gggtgagaat cggacaccgc tgccggcgaa gggaccggct
     4501 gaaccctggc gcacgcggct gccgcgcaac agaggacgat cagcgtggtt gaaccgacgc
     4561 ggcgcaagcc gtgatcaggc actcggtttc ccgggttgac tgggatcgga ctttgacgat
     4621 gcgccaagat cggtcgggtc ttgcacgccc ttcttgaatc cacggatcgc ctttcccaac
     4681 ccggcgccga tctcgggaag tcggctggcg ccaaagatca agagcactaa cacgaggatc
     4741 accaagagct ccggcatacc aagaccaaac atcgttcgtc tccagcaacg attcgattat
     4801 aggattcggt tcaaaaaggt gtcaagaaat ccgcggcgcg cggatccgac tcatcccggg
     4861 ctgggtcgtt gcgcggtctc gaggttctcg aaccgagtat accggtcgag gaaggccagc
     4921 ctgacatccc cgatcggccc
//
LOCUS       DBNGTT010000265         1735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2868217, whole genome shotgun sequence.
ACCESSION   DBNGTT010000265 DBNGTT010000000
VERSION     DBNGTT010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2868217"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="AC16BC_05935"
     CDS             complement(<1..1701)
                     /locus_tag="AC16BC_05935"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012340.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIR3241195.1"
                     /translation="MPNFIRYSGAGQSSLRALDVRPDDTDARDYIFQPSLTLLGQQVD
                     HRGLAPVLDQGEEGACVGFALATVINVSLACREQPRGRRARGTMFEEVSARMLYEMAR
                     RYDEWKGEQYEGTSLRGAMKGWHKHGVTTEALWRSRPKRNGDAKIDRELTPERAQDAL
                     RRPIGAYFRIVDSDVGHLQAAVAEGDAVLASAWVHSGWEDERLLDSKGSLKVKRIPHK
                     TGRGGLHAFAIVGYTPDGFLIQNSWGPRWGTKGHALLTYDDWFENRQDAWVARPGPET
                     KDSTGLSKIFTVAFAGGGNETRAGTAASGLDLDPEVLPHLINTGDRGVLSSGGRLTTK
                     ASEIPDMARRVLTSRPLRDGFRHVVLYAHGGLNSETDSAATANRLWALCRQKDVCSYF
                     FIWESGLQESVRGWLKSDDDASGPARFSWSDAWDHIKKGAGNVVRGAQKVLGASLAPM
                     VREVFWDEMKGRAQGASSAKGGGALFTDELFNVMAQIPGDKYKLHLVGHSAGSIYLGW
                     LYQSVLRQRLAGPQNIKLGSIQFMAPAITIDRAQKAFCLNGQGPVSKDKFVVYTLKPK
                     D"
BASE COUNT          339 a          590 c          465 g          341 t
ORIGIN      
        1 atccttcggt ttcagcgtat agaccacgaa cttgtccttc gagactgggc cctgaccgtt
       61 gaggcaaaag gctttctgcg ctcgatcaat ggtaatcgcg ggcgccatga actggatgga
      121 gccgagcttg atattttgtg gcccggccaa ccgctgcctg agtacagatt gatagagcca
      181 tccgaggtag atgctccccg cgctgtgacc aacaagatga agcttatatt tgtctcccgg
      241 tatctgagcc atcacgttga acagctcgtc ggtgaacagc gcgccgccac ccttggccga
      301 cgaagcccct tgagcccgcc ccttcatttc gtcccaaaag acctcgcgga ccatcggagc
      361 caaactggca ccaagcactt tctgcgcgcc gcgcacaacg ttaccggctc ccttcttgat
      421 gtgatcccac gcgtcactcc aactaaatcg cgcggggcct gacgcatcgt cgtcgctctt
      481 gagccatccg cgtaccgact cttgcagccc cgactcccaa ataaagaagt aggaacacac
      541 gtccttctgt cggcacaacg cccagaggcg gttcgcggtc gcggctgagt cggtctcgga
      601 gtttaggccc ccgtgggcat acaacaccac gtgtcgaaat ccgtctctca acggtcgcga
      661 cgtgaggacg cgccgcgcca tatccgggat ttccgaggct ttcgtggtca gtcgcccccc
      721 ggatgacagc actcctcgat cgcccgtatt gatcaaatgg gggagcacct cgggatcgag
      781 atccaagccc gaggcggcag tgcccgccct cgtctcattc ccaccgccgg caaaggcgac
      841 ggtgaaaatc ttcgacagac ccgtcgagtc cttggtttca ggacccggtc gagcgaccca
      901 ggcatcctgc cggttctcaa accaatcatc gtaggtgagc aacgcgtgcc ccttcgtgcc
      961 ccatctgggc ccccacgaat tctggatgag aaaaccatcc ggcgtatagc cgacaattgc
     1021 aaacgcgtgg aggccgccgc gaccggtctt atgcggaatg cgcttcacct tcaacgaccc
     1081 tttggagtcc agcagccgct catcctccca gcccgagtgg acccaggccg atgcgagaac
     1141 ggcgtcccct tccgcgacag cggcttggag atggcccaca tcgctgtcga caatgcggaa
     1201 gtaggcgccg atcggccggc gcaacgcatc ttgggcccgc tccggcgtca actcgcgatc
     1261 gatcttcgcg tcaccgtttc gtttcggccg acttctccat aaggcctccg tcgtcacccc
     1321 atgcttgtgc catcccttca tggccccgcg caggctggtt ccctcgtact gttcgccctt
     1381 ccattcgtcg taacggcgcg ccatctcgta caacatgcgc gcgctcactt cttcgaacat
     1441 tgttccccgg gcccgtcggc cacgaggttg ctcgcggcaa gccagactca cgttgatcac
     1501 cgtcgcgagc gcgaacccga cacacgctcc ttcctcccct tgatcgagca cgggagccaa
     1561 cccgcgatga tcgacctgct gacccaacag ggtcagcgag ggctggaaaa tgtaatcgcg
     1621 cgcatccgtg tcatcgggcc gaacatccaa cgcacgcaac gaactctggc cagctccgct
     1681 gtagcgaatg aaattaggca tggcattctc ctctcacgtt ggtggagcta gggaa
//
LOCUS       DBNGTT010000266         4132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_312988, whole genome shotgun sequence.
ACCESSION   DBNGTT010000266 DBNGTT010000000
VERSION     DBNGTT010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_312988"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..745
                     /locus_tag="AC16BC_05940"
     CDS             254..745
                     /locus_tag="AC16BC_05940"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3241196.1"
                     /translation="MPPTVQSYPDAARVVAAGADLVTHEIARAVAARGRAWVVLAGGE
                     TPRALYERLAAAHEAVPWGNIWWCFGDERWVPRTDPLNNFAMAERVLFSQTPIHRDHV
                     LAVSTDSNGPSAGARTYEQALRAHFPDAEWPKFDVAGASARTDTPPRCFPETRRWTSA
                     QPG"
     gene            646..996
                     /locus_tag="AC16BC_05945"
     CDS             646..996
                     /locus_tag="AC16BC_05945"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3241197.1"
                     /translation="MAQVRRGGGLGADGHTASLFPGDPALDERAAWVTTTRAGKPVPD
                     RVTLTAPAFAHAGVMIFLVTGAAKAHAVTATLGAPNPARWPVQAVMPRSGPCHWLLDQ
                     EAACAFPPGVPPAA"
     gene            1053..1718
                     /locus_tag="AC16BC_05950"
     CDS             1053..1718
                     /locus_tag="AC16BC_05950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3241198.1"
                     /translation="MVRLEGVSKIYPLGAQRIWALRDLTLEIPSGEFHVVMGPSGSGK
                     STLLHLIAALDLPTNGTVWLDGRATTACSDVELTRLRRDRIGLVFQSFHLLPYLTVRE
                     NVALPRLLQRAPARQVRAETDELLKAVGLAQRAEHWPHELSGGEAQRTAIARALITRP
                     ALFLADEPTGNLDSARGREILDLIARLTAASGVTVVLVTHDPDAAKVGHRVTHLKDGR
                     IDG"
     gene            1715..>4132
                     /locus_tag="AC16BC_05955"
     CDS             1715..>4132
                     /locus_tag="AC16BC_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3241199.1"
                     /translation="MSPLIRFTAFRHLRHAALRTALTVSGLALGVALYVAIRLCNASV
                     DASFRDTVAGLSGAATLEVIGGEDGVDEDLFDAVARTPGVVAAAPVIIRHVALDDRTS
                     LIVLGVDLLSDGPFRSYETIGSADPLDAWVLDPTAVLLTARVAAARGLRPGETLTVLD
                     RDRPITLKVVGVTADQRLSRAWGGAVAVMDIAAAQWTFDRVGRLDRIDVLTTPDEADA
                     VHARLVAALPPRLVVQRPDRQVAHAQQVVRSFQVNLTALSAIALFVGLFLIYNTMTHA
                     LLRRRAEVGLLRALGVSRSRLFGALVVEAGVFGVVGGAVGVPLGWVLARAAVGTVSAT
                     VETLYGAGPAQPVVLTASLALEGLSLGCVVAVAASLVPIAEALTTVPREVLHAGWVQR
                     RRRIRAGRLAAIGLGWIVLAWALAQAGPIAGVPVFGYGAALCLVLAGVFAMPLLTVGL
                     GRVVRPLAMRMGWVEAQLAAGALGAAPGRTAVAAGALMTALAMMVSVVVMVGSFRRTV
                     EQWIDATINADLYVSPASRPAVGPSAHFADDAIVGRIAAVPGVAAVDPYRQVPATDHG
                     QSMLLSARDLTIVRERSGMQFIAGNSSELLARLARGEGIALSEVLASRLGIHAGDTLI
                     LPTFDGDRLFQVLGVFYDYATDGGRVLMDRSLWRRHWRDQGVTALAVYVDDGVDPDDV
                     RAAIETTLAPAQRVSILSNRGLRTDVLEVFDQTFAITRALDVVAMTVAALGVAGAVLA
                     IVMERRREIAVLRALGATRAQISGVVVWEAALIGLLGTGLGVAVGFGISVVLITVVNV
                     QSFGWTIL"
BASE COUNT          558 a         1368 c         1485 g          721 t
ORIGIN      
        1 aacggggttc gaacccgtgt ctccggcgtg agaggccagc gtcctaggcc actagacgat
       61 gggaccgttc ggcgacttcc tacaaaatca agaggttgga gtatagcgat ccgaggccgg
      121 aagttcaact gtgctcgtcg ctgtccgtgg cgtggtcagg gaattctgga gcgaaccgtg
      181 gcgcaccaac ggcttgttgc aagaagggga atccgctacg atcatggcga accctgaccg
      241 acgcggagca tccatgcccc cgactgttca atcgtatccc gacgcggcgc gcgtcgttgc
      301 cgccggggcc gacctcgtga cacacgaaat cgcgcgggcg gtcgcggccc gaggccgcgc
      361 ctgggttgtc ctggccgggg gcgagacccc tcgcgcgctg tacgaacggt tggccgcagc
      421 ccacgaagca gtgccttggg ggaacatttg gtggtgcttc ggggacgagc ggtgggtgcc
      481 ccgcaccgac ccattgaaca acttcgcgat ggcggagcgc gtcttgttct cccaaacgcc
      541 gattcatcgg gatcacgttc tcgccgtatc gaccgactcg aacggtccgt ccgcgggcgc
      601 gcggacatat gagcaggcct tacgcgcgca ctttcccgac gccgagtggc ccaagttcga
      661 cgtggcgggg gcctcggcgc ggacggacac accgcctcgt tgtttcccgg agacccggcg
      721 ctggacgagc gcgcagcctg ggtgaccacc acgcgcgcgg gcaaacccgt gccggatcgt
      781 gtcaccctca ctgccccggc gttcgcccat gctggggtga tgatctttct ggtgacgggc
      841 gcggccaaag ctcacgcggt cacggccacg ctgggcgcgc ccaacccggc ccggtggccc
      901 gtgcaggcgg tgatgccccg atcaggccct tgccactggc tgttggatca ggaagcggcc
      961 tgcgcgttcc cgccgggagt gccgccagcg gcgtaaccat cgcgcagaac gtcgcccttg
     1021 cctcgcggca acgtcttccg tatcctgctc gcgtggtccg tttggaaggt gtcagcaaga
     1081 tctacccctt gggcgcgcag cggatctggg ccctgcgcga cctgacgctc gagatcccgt
     1141 ccggagaatt ccacgtggtg atggggccga gcggctccgg caagagcacg ctccttcacc
     1201 tgatcgcggc gctggatctc cccacgaatg gcacggtctg gctggacggg cgcgccacca
     1261 ccgcctgctc ggacgtggag ttgacccgcc ttcggcgcga ccgcatcggc ctggtgtttc
     1321 agtcgttcca cctgctgccg tatctcacgg tgcgcgaaaa cgtggcgctt ccgcgcctgt
     1381 tgcagcgggc tcccgcgcgc caagtccgcg cggaaaccga cgaactgctg aaggccgtcg
     1441 ggctcgccca gcgcgcggaa cactggcccc atgagctgtc aggcggcgag gctcagcgta
     1501 ccgcgatcgc gcgggcgttg atcacccgtc cggccttgtt ccttgccgac gaaccgaccg
     1561 gcaacctgga ctccgcgcgg ggacgggaga tcctcgacct gatcgcgcgc ctgaccgcgg
     1621 cctcgggcgt gaccgtggtg ctggtgaccc acgatccgga cgcggccaag gtcggccacc
     1681 gcgtgacgca cctcaaagac ggcaggatcg acgggtgagc ccgctgattc gattcaccgc
     1741 cttccgtcat cttcggcacg ccgcgctccg gaccgcgctc acggtgtcgg ggctcgcgct
     1801 cggggtggcg ctgtacgtgg ccatccgcct gtgcaacgcg agcgtggacg cctcgttccg
     1861 cgacaccgtc gccgggttga gcggggccgc gactcttgaa gtcatcgggg gcgaagacgg
     1921 ggtcgacgaa gatttgtttg acgcggtagc gcgcacaccc ggcgtcgtcg cggcggcgcc
     1981 cgtgatcatc cggcacgtgg cgttggacga ccggacatcc ctcatcgtgt tgggcgtgga
     2041 cctgttgagc gatggcccgt ttcgttccta tgagacgatt ggttccgccg acccgctcga
     2101 cgcctgggta ctcgatccca cggccgtgct cctgaccgcg cgtgtggccg cggcccgcgg
     2161 cctccgtccc ggcgagacgt tgacggtcct ggaccgcgac cggcccatca ctctcaaggt
     2221 cgtcggcgtg acggcggatc agcggctgag ccgggcatgg ggtggggccg ttgcggtgat
     2281 ggacatcgcc gccgcgcaat ggacgttcga tcgagtgggt cgtctcgacc gcatcgacgt
     2341 gttgacaacc cccgacgagg cggacgcggt ccacgcccgg ctggtcgccg ccctgccgcc
     2401 gcgcctcgtc gtgcagcggc ccgatcgcca agtggcgcac gcccagcagg tggtccgctc
     2461 gtttcaggtg aacctcaccg cgcttagcgc aatcgccttg ttcgtgggac tgttcctgat
     2521 ctacaacacg atgacgcacg cgctgttgcg ccgccgcgcc gaggtcggcc tgctgcgcgc
     2581 gttgggcgtc agccggtcac ggttgttcgg cgcgctcgtg gtcgaagccg gggtgttcgg
     2641 agtcgtgggc ggcgcggtcg gcgtgcccct gggatgggtc ctggcccgcg ccgccgtggg
     2701 cacggtctcg gcaacggtcg agaccttgta cggcgcgggc cccgcccaac ccgtcgtgct
     2761 gaccgcgagc ctcgcgctgg aagggctgag cctcggctgc gtggtcgcgg ttgcggcctc
     2821 gctcgtgccg atcgccgaag ccttgaccac cgtcccccgc gaggtgctgc acgcggggtg
     2881 ggtgcaacga cggcgccgta tacgggccgg ccgactcgct gcgatcgggc tgggctggat
     2941 cgtcctggcg tgggcgctcg cgcaggcggg ccccattgct ggggttccgg tcttcggcta
     3001 cggagcggcg ctctgtctgg tgttggcggg agtcttcgcc atgccgttgc tgaccgtggg
     3061 gctggggcgc gtggtccggc cgctcgccat gcggatggga tgggttgagg cgcaactcgc
     3121 ggcgggcgcg ctcggcgccg cgccgggacg gaccgcggtg gccgccggag ccctgatgac
     3181 cgcgctcgcc atgatggtca gcgtggtggt gatggtcggg agttttcggc gcacggtgga
     3241 acaatggatc gacgcgacca tcaacgcgga cctgtacgtg agccccgcgt cgcggcccgc
     3301 ggtcggcccc tccgcgcact ttgccgacga cgccattgtt ggccgaatcg ccgcggtgcc
     3361 cggcgtcgcc gcggtcgacc cctaccgtca ggtgcccgcc acggaccacg gccaatccat
     3421 gctcttgtcc gcacgggacc tgaccatcgt gcgcgagcgc agcgggatgc agtttatcgc
     3481 cggcaattcg agcgagctgc tcgcgcgact ggcgcgcgga gaggggatcg cgctgtcaga
     3541 ggtgctggcc agccggctcg ggatccatgc gggcgacact ctgatcttgc ccacgttcga
     3601 cggtgacagg ctgtttcagg tgcttggagt gttttatgat tacgcgaccg acggcgggcg
     3661 ggtgctcatg gaccggtccc tgtggcgtcg gcactggcgc gatcagggcg tcaccgcgtt
     3721 ggccgtgtac gtggacgacg gggtcgatcc cgacgatgtc cgcgcggcca tcgagacgac
     3781 gctggcgccc gcgcaacggg tctcgattct ctcgaaccgc gggctgcgga ccgacgtgtt
     3841 ggaggtgttt gaccagacgt ttgccattac gcgcgcgctc gatgtggtgg ccatgacggt
     3901 cgcggcgctc ggggtcgccg gcgcggtgtt ggcgatcgtg atggaacggc ggcgggaaat
     3961 cgccgtgctg cgggcgctgg gcgccactcg agcccagatc agcggcgtcg tggtatggga
     4021 agcggccttg atcggactgt tggggaccgg gctgggcgtg gcggtggggt tcggcatttc
     4081 ggttgtcttg atcaccgtgg tcaacgtgca atcatttggc tggacgattc tg
//
LOCUS       DBNGTT010000267         2032 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2996528, whole genome shotgun sequence.
ACCESSION   DBNGTT010000267 DBNGTT010000000
VERSION     DBNGTT010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2996528"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="AC16BC_05960"
     CDS             complement(<1..534)
                     /locus_tag="AC16BC_05960"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3241200.1"
                     /translation="MYTVRVATTEEFHASRAQWNALVGAMNYPSIFCTWEWVAAWMDV
                     YAADSEPVILMAHRDGELIGILPLCRSSVTLRNGWMTGRVLSFCGSLDLYPDHLDLIA
                     SRDESESCVNAILEFLGRELTDWDVLSLPYLAGDSNLVSLLGHGRHRFSIDLRPVSSA
                     RFIALAGDYDRFLLRFDG"
     gene            complement(534..1544)
                     /locus_tag="AC16BC_05965"
     CDS             complement(534..1544)
                     /locus_tag="AC16BC_05965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3241201.1"
                     /translation="MYRALKQLAKQTAYGWLSTDTVLGYWRARTLREGAVVLMYHELA
                     ADDEDIESWLVVKRNDFCRQVEYLKQHYDVVGFDDAIRLMSAGEERERPLAVLTFDDG
                     YLGNLEVLAPVLAEFECPATVFVATGAVQDQSLYWYDRLISRFQGQDALLLDLTDRSL
                     GRYRINHRHGPENWNEIERLLTDLKRLAPDHRRENVEAILDRVPVGSHGHERGIRYLA
                     VDDVRALSHSKWVTIGAHSHCHNLLPQLSVDDARRSIETSKRLLESWTGRPVTMFSYP
                     NGDYSGPVIDAVRAAGFQAAVTTRPRPWTAGDSVFEIPRIGVGRYDSLTAFKVRGAGM
                     HV"
     gene            complement(1556..>2032)
                     /locus_tag="AC16BC_05970"
     CDS             complement(1556..>2032)
                     /locus_tag="AC16BC_05970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3241202.1"
                     /translation="EEILMRPKRKFRLTKTYLLGFFKPAPRRPDAMILKECAAYFPGD
                     LERYKGLMTKWVARIREAGIEVMVATVVPVTERRAGEQPGKIEGIRAYNDWVREFAQR
                     ERIPVLDLEAAMATQDGRRFLRDEWTSGDGLHLNAQAYQALDQALADLLAQRQQAS"
BASE COUNT          395 a          687 c          590 g          360 t
ORIGIN      
        1 gccgtcgaac cgcagcagga atcggtcgta gtctccggcg agggcgatga accgggccga
       61 cgataccggc cgcaggtcga tcgagaaccg atgtcgtccg tgcccaagca gcgacaccag
      121 attggaatcc cccgccagat acggcagcga gagcacgtcc cagtccgtca attcccggcc
      181 gagaaactcc aggatcgcgt tgacgcatga ttctgattcg tctcgcgacg caatgagatc
      241 caggtggtcc gggtacagat ccaggctccc gcagaagctc agcacgcgcc ccgtcatcca
      301 cccgttacgc agcgtcaccg acgagcggca caaggggagg atgccgatca gttcgccgtc
      361 gcggtgcgcc ataaggatca ccggctcgga atcggctgcg tacacgtcca tccatgccgc
      421 cacccactcc cacgtgcaaa aaatcgacgg gtaattcatg gcgcccacga gcgcgttcca
      481 ttgcgcccgg ctggcgtgaa attcctccgt ggtggccact cgaaccgtgt acatcagacg
      541 tgcatccccg ctccgcgcac cttgaacgcg gtcagcgaat cataccgccc gaccccgatc
      601 cgagggatct caaacaccga gtcgccagcg gtccacggtc gcggccgggt ggtgacggcg
      661 gcctggaatc ccgcggcccg caccgcgtcg atcactggcc cgctgtaatc accattggga
      721 taggaaaaca ttgtcacggg tcgcccggtc cacgattcca gcagtcgctt ggatgtctca
      781 atgctgcgtc gcgcatcgtc gaccgacagt tgcgggagga ggttatggca gtgggagtgc
      841 gcgccgatcg tcacccactt cgagtgtgac aaggcccgga cgtcgtcaac cgcgaggtaa
      901 cggattccgc gctcatgtcc gtgcgacccg accggcacgc gatcgagtat cgcctcgacg
      961 ttctcccggc gatgatcggg ggcgaggcgc ttcagatcgg tcaagaggcg ttcgatctcg
     1021 ttccagtttt caggaccatg gcggtgattg atgcggtagc ggcccaacga gcggtccgtg
     1081 aggtccagga gcaaggcatc ttgcccctga aatcggctga tcagccgatc gtaccaatag
     1141 agggattgat cctggaccgc gcccgtggcc acaaacaccg tggcggggca ttcgaactcc
     1201 gcgagaaccg gcgccaggac ctccagattg cccagatacc cgtcgtcaaa cgtgaggacg
     1261 gccaacggac gctcccgctc ttcaccagca ctcatcaggc gaatcgcatc gtcgaaaccg
     1321 accacgtcat aatgctgctt cagatattcc acctgtcggc aaaagtcgtt gcgcttcacg
     1381 acgagccacg attcgatgtc ctcgtcgtcc gcggccagtt cgtgatacat cagcaccacc
     1441 gcgccctcgc gcaacgtccg cgctctccaa tagcccaaca ccgtgtcggt cgagagccac
     1501 ccgtacgcgg tctgtttggc cagttgtttg agggcgcgat acatcaggaa tccgctcagg
     1561 acgcctgttg gcgctgcgcc agcagatccg ccagcgcctg gtccaacgcc tgataggcct
     1621 gcgcattgag atgcagcccg tcgccgctcg tccactcgtc cctcaaaaac cgccggccgt
     1681 cctgggtcgc cattgcggcc tcaagatcga gcaccggaat ccgctcgcgc tgcgcgaact
     1741 cgcggaccca atcgttgtac gcgcggattc cctcgatctt tccgggctgc tcacccgcgc
     1801 gtcgctccgt caccggcacc acggtcgcca ccatcacctc gattccggcc tcacggatcc
     1861 gcgcgaccca cttcgtcatc aagcccttgt agcgttcgag atcgccggga aaataggcgg
     1921 cgcactcctt gagaatcatg gcgtcgggac gccggggggc gggtttgaag aaccccaaca
     1981 gatacgtctt ggtcaggcgg aattttcgtt tcggccgcat caaaatctct tc
//
LOCUS       DBNGTT010000268         2030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2613444, whole genome shotgun sequence.
ACCESSION   DBNGTT010000268 DBNGTT010000000
VERSION     DBNGTT010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2613444"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1817
                     /locus_tag="AC16BC_05975"
     CDS             312..1817
                     /locus_tag="AC16BC_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HIR3241203.1"
                     /translation="MTASIERGISHRTDSLPWRHSFASLPPPFYTRVMPTPLPSPYLV
                     AGSVSAAAMIGLDPAEFTSQEFVESFSGNRLPAGATPVAAVYSGHQFGIWAGQLGDGR
                     AILLGDVPARGAGHPGVMELQLKGAGQTPYSRMGDGRAVLRSSIREFLCSEAMAGLGI
                     PTSGALCVTGSDRRVMRETPETAAVVTRMAPTFIRFGSFEHWFYNERLDALRALADYV
                     IDRFYPDLRQAPNPYQALLAEVTRRTARLTAQWQAVGFMHGVMNTDNMSILGLTIDYG
                     PFGFMDAFDPHHICNHSDHHGRYAYDRQPDVAYWNCVRLGRALLPLIGHEDDARAALE
                     PFVPEFSAAWEDLLRAKLGLRTAMPDDADLLNAMFAMLQAGRVDFTLFFRRLGDLHIE
                     NPAADEPLRDLVIDRAAFDAWATRYRLRLRQEGSVDARRREAMHTVNPKYVLRNYLAQ
                     VAIDKAQQKDFSEIERLQAILENPFDEQPEHDAYAKPPPDWGAHVTVSCSS"
BASE COUNT          336 a          701 c          637 g          356 t
ORIGIN      
        1 ccagcagact tcgcacgacg cggatgtggc tcacgtgttt catggcgatc ctcctcgtct
       61 agagtctaga gcctcaaggt acccgccaga agcagcggaa actataccac caggggacat
      121 gctgacaagc gcgcgaccct gtcgcagacc gcggcacagc ggtgaatacg gtgtctggat
      181 ggtcaccctc cggccgttcg caacttcctc cggccgcacc ggacgaggct ggggtataat
      241 ggcgcagcgc tggtgcccgc ccgagtccgt ccgaacgatg tggatcgcac cggcggacct
      301 gatcgaccga catgaccgcg tccatcgagc gaggcatttc ccatcgcacg gattccttgc
      361 cgtggcgtca ctcgttcgcg agtttgccgc ctccgttcta cacccgggtc atgcccacac
      421 ccctgccctc gccgtatctg gtggcgggga gcgtctcggc cgcggccatg atcggcctgg
      481 accccgcgga gttcacgtct caggaattcg tcgagagttt ttcgggcaac cggcttccgg
      541 ccggcgccac gccggtcgcg gccgtgtatt cggggcacca gttcggcatc tgggccggac
      601 aactgggcga cggtcgggcg atcttgctgg gcgacgtgcc cgcgcgcggg gcggggcatc
      661 ccggagtgat ggagttgcag ctcaagggcg cgggccagac cccgtattcg cgcatgggcg
      721 acggccgcgc ggtattgcgc tcgtccatcc gcgagtttct ctgttcagag gccatggccg
      781 ggctcggcat tcccacctcc ggcgcattgt gcgtgaccgg ctcggaccgc cgcgtgatgc
      841 gggagacgcc ggagaccgcg gccgtggtca cgcgcatggc tccgacgttc atccgtttcg
      901 gctcgttcga gcactggttt tacaacgagc ggttggacgc gctccgcgcg ctcgcggact
      961 acgtgatcga tcggttctac ccggaccttc gccaggcccc gaacccgtac caggccttgc
     1021 tcgcggaggt cacgcgccgc accgcgcgct tgacggcgca atggcaagcg gtcgggttca
     1081 tgcacggggt catgaacacg gacaacatgt cgattctcgg cctcaccatc gactacggcc
     1141 cgtttggctt catggacgct ttcgacccgc accacatctg caatcattcg gaccaccacg
     1201 ggcgctacgc ctatgaccgg cagcccgacg tcgcgtactg gaactgcgtg agactgggcc
     1261 gggccttgtt gcccctgatc ggccatgaag acgacgcgcg cgccgcgctc gaaccgttcg
     1321 tccccgagtt ttccgccgcg tgggaggatc tcctgcgcgc caagctcggg ctgcggaccg
     1381 cgatgcccga cgacgccgac ctgctgaacg cgatgttcgc gatgttgcag gccggccgcg
     1441 tggacttcac cctgttcttc cggcgattgg gcgatttgca catcgagaac cccgcggcgg
     1501 acgaaccctt gcgggatctg gtaatcgacc gcgcggcgtt cgacgcgtgg gcgacacggt
     1561 atcgtttgcg gttacgacag gagggcagcg tggatgccag gagacgagag gccatgcaca
     1621 cggtgaaccc caagtacgtg ttgcgcaact acctggccca ggtcgcgatt gataaggcgc
     1681 aacagaaaga tttttcagaa atcgagcggc tccaggcgat ccttgaaaac ccgttcgacg
     1741 aacaaccgga acacgacgcc tacgccaagc ctccgcctga ttggggcgcc cacgtcacgg
     1801 tcagttgctc gtcataaccg cacggcaacc atccgcgcac ccaaccgtcc gcgactccat
     1861 tggcctccca ccaaccgtct gatccgactg atctctccac gctcccctgg tacggcgccg
     1921 accgcacgct ccccgcgttg cgattcgccc ccgggcgtga tccgcgtccg gaggcacgcc
     1981 gcgatgcccc tgacatccac cagccctgga cgcccgagca gtggcggact
//
LOCUS       DBNGTT010000269         3861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3508029, whole genome shotgun sequence.
ACCESSION   DBNGTT010000269 DBNGTT010000000
VERSION     DBNGTT010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3508029"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC16BC_05980"
     CDS             complement(<1..371)
                     /locus_tag="AC16BC_05980"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HIR3241204.1"
                     /translation="MSHPLHPVVVHFPIALLITAIAFEALELVLKRDFLQEAATWLLG
                     LGFLGGLVAAATGVLAEDAAEDAGVPESAIETHESLAFAALAVFALLIVIRWLQRTRK
                     IPNTVFLGVGIVGVVLIGLTG"
     gene            complement(434..1651)
                     /locus_tag="AC16BC_05985"
     CDS             complement(434..1651)
                     /locus_tag="AC16BC_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241205.1"
                     /translation="MAARGRTARTLWAFSVAIFCLAGVTGRADANPGFARKYSVGCQM
                     CHIAFPKLNNFGEQFARNGYQFPNEEPAKNSVRLGDERLLLPTSPNFAIRADGFFRVR
                     NDTAINNDIESPWILKLFSYGLLAKDIAYYFYFLASEGGDVVGPEDAFLYFNNIAGGL
                     DWDAVVGQFQVMDSFYAREQRLTFQDLSIYKANVMETVGAGGSFDLTYDRGAFTTFST
                     GPLYTMVGVTNGNGLRSADANDNFDDNGYKNKMGRVALGLPGATVGVFGYHGKTNPIA
                     PGTVPNRFNRWGPDLTLHLGPRADLRATWLWGRDEHPTFSTTDTPVSHNGGFAEFDVH
                     LNDRWTGVLLYNEVVADELPELQVRTLTANLTYYKMRNLKFMAEYTHDLQPRTSVHPG
                     KTHTGVLGVVLAY"
     gene            complement(1709..3169)
                     /locus_tag="AC16BC_05990"
     CDS             complement(1709..3169)
                     /locus_tag="AC16BC_05990"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3241206.1"
                     /translation="MPYTDLREFLDALEKAGELVRVTAEVDPRLEVTEILNRLLAREG
                     PAVLFERVKGSSIPLLANLFGTVKRVAMGLETDEAGLEEIGKFLAFLQHPEPPKGFVD
                     AIKHLPFYGRILHLAPKTVSRAPCQEVVLTGDRIDLGLLPIMFCWPDDGGPQINWPLV
                     VTKSPHGGKYNAGIYRLQVMGKDRTAMRWLKHHGGAQHWREWMAAGKTMPVAAVLGCD
                     PGTTIAAVTPVPEQIGEYHFAGLLRRKNIELVRCKTIDLEVPASAEIVIEGEVSPGDM
                     CDEGPHGDHTGYYNPVESFPVFRVTAITHRRDPIYMTTVTGRPPREDAVVALALNRIF
                     LPMIKTTFPEIVDFHLPMEALSYRVAVIAMKKEFPGHAKRVMMGLWGFLKQFLYVKFV
                     IVVDPTIDVRTWEDVIWALSTNVDPRRDVTIIDHTPIDYLDFASPLPELGSKMGIDAT
                     AKIPPETSREWGKPIIMDPDIIERVTRRWREYGFSP"
     gene            complement(3177..>3861)
                     /locus_tag="AC16BC_05995"
     CDS             complement(3177..>3861)
                     /locus_tag="AC16BC_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR3241207.1"
                     /translation="VLNDVADRHLDGRVDRTKDRPLASGRLTAAEALAVGAGLSVIAL
                     LLTLALNPLAVALSVAGFALAAVYPLMKRVIAVPQAVMGAAFGWGALLAWAAVRNEVG
                     TPAIVIFLATVCWAAGYDTIYALMDRDDDEKIGVRSSAIFFGRYAWAGVGGLLGLAVI
                     GMIVLGVIAPLGPVYVATIAAVAAAFGYQTWRLRQPLDRAAIFALFRSHAWIGVVILL
                     GIIVDLSIR"
BASE COUNT          656 a         1233 c         1310 g          662 t
ORIGIN      
        1 tacccggtca atccgatcaa caccacgccg acgatcccga cgcccaggaa cacggtgttc
       61 gggatcttgc gtgtgcgctg cagccagcgg atcacgatga gcaaggcgaa cacggccagc
      121 gcggcgaagg cgagcgactc gtgtgtttcg atggcgcttt ccggcacccc ggcgtcctcc
      181 gcggcgtcct ccgccaggac tccggttgcc gcggcgacca gtccgcccag aaagccgagg
      241 ccgagtaacc acgtcgcggc ctcttggaga aagtcccgct tcaagaccaa ttcgagcgct
      301 tcgaacgcga tcgcggtgat caaaagcgcg atcgggaagt gtacgacgac cgggtgcagc
      361 gggtgagaca tgaaccgtcc tttctccgaa tgccgcgcga gtgcctcgcg acgcgaagcg
      421 ggcccgaggc cgcctagtat gccagaacca ctcccaaaac gcccgtgtgc gttttccccg
      481 ggtggaccga ggtccggggc tgcaggtcgt gcgtgtactc cgccatgaat ttcaggttcc
      541 gcatcttgta gtacgtgaga ttggcggtca gcgtgcggac ctgcaattcc gggagctcgt
      601 ccgccaccac ctcgttgtac agcaacacgc cggtccagcg gtcgttcagg tgcacgtcga
      661 attccgcgaa cccgccgttg tgggacacgg gggtgtcggt cgtcgaaaag gtggggtgtt
      721 cgtcgcggcc ccagagccag gtggcgcgca ggtcggcgcg gggcccgagg tgcagggtga
      781 gatccgggcc ccaccggttg aagcggttgg gcaccgtgcc gggggcgatc ggattggtct
      841 tgccgtggta gccgaacacg cccaccgtgg cgccgggcag tccgagcgcg acgcgcccca
      901 tcttgttctt gtacccgttg tcgtcgaagt tgtcgttggc gtccgcggac cgcaggccgt
      961 tgccgttggt gacgccgacc atggtgtaca gcggtccggt cgagaacgtg gtgaaggcgc
     1021 cgcggtcgta cgtcaaatca aagctgccgc ccgcgccgac cgtctccatc acgttggcct
     1081 tgtagatcga gaggtcctgg aacgtgagcc gttgctcgcg cgcgtaaaac gagtccatga
     1141 cctggaactg gccgaccacc gcgtcccagt ccagtccgcc cgcgatgttg ttgaagtaga
     1201 ggaacgcgtc ttccggaccc accacgtcgc cgccctccga ggccagaaag tagaagtaat
     1261 aggcgatgtc cttggccagg aggccgtagc tgaacagctt caggatccag ggcgactcga
     1321 tatcgttgtt gatcgcggta tcgttgcgca cgcggaaaaa tccgtcggct cggatcgcga
     1381 agttggggct ggtggggagc agcaggcgtt cgtcgcccag tcgcaccgag ttcttcgcgg
     1441 gttcttcgtt ggggaactgg tagccgttgc gcgcgaactg ctcgccgaaa ttattgagct
     1501 tggggaacgc gatgtggcac atctggcatc cgacgctgta tttgcgggca aagcccgggt
     1561 tggcgtcggc gcgccccgtc acgccggcca gacagaaaat cgccacgctg aaggcccaga
     1621 gcgtccgtgc ggtgcgtccc cgtgcggcca tgtgtcctcc ttgtccggtg cggcggccgc
     1681 ctcccgcggc cgcgtgatca atccgtgctt acggcgaaaa cccgtactcg cgccaccgcc
     1741 gcgtgacgcg ttcgatgatg tcgggatcca tgatgatcgg tttgccccat tcgcgcgacg
     1801 tctcgggcgg gatcttggcg gtggcgtcga tgcccatttt cgaaccgagt tccggcagcg
     1861 gggacgcgaa gtccaggtag tcgatcggcg tgtggtcgat gatcgtcacg tcgcgccgcg
     1921 ggtccacgtt ggtggacagg gcccagatca cgtcctccca cgtgcgcacg tcgatcgtgg
     1981 gatcgaccac gatcacgaac ttcacgtaca ggaattgttt gaggaagccc cacagcccca
     2041 tcatcacgcg tttggcgtgg ccggggaact ccttcttcat ggcgatcacc gcgacgcgat
     2101 aggagagggc ttccatgggc aggtgaaagt ccacgatctc ggggaacgtg gtcttgatca
     2161 tggggaggaa gatgcgattg agcgcgagcg ccaccaccgc gtcttcgcgc ggcggccggc
     2221 ccgtcacggt cgtcatgtag atcgggtcgc gccggtgggt gatggcggtg acgcggaaca
     2281 ccggaaacga ttcgaccggg ttgtagtacc cggtgtggtc gccgtgcggg ccctcatcgc
     2341 acatgtcgcc gggagacacc tcgccctcga tcacgatctc ggccgaggcc ggcacttcca
     2401 ggtcgatggt cttgcagcgg accagttcga tgttcttgcg gcgcagcagc cccgcgaaat
     2461 ggtattcgcc gatctgttcg ggcacgggcg tgaccgcggc gatcgtggtg cccgggtcgc
     2521 agcccaacac cgcggccacc ggcatggtct tgcccgcggc catccactcg cgccagtgct
     2581 gggcgccgcc gtggtgtttg agccatcgca tcgcggtgcg gtccttgccc atgacttgca
     2641 atcggtaaat gcccgcgttg tatttgccgc cgtgcggcga cttggtcacc accaagggcc
     2701 agttgatctg gggcccgccg tcgtcgggcc agcagaacat gatcggcagc agtcccaggt
     2761 cgatccggtc gccggtgagc acgacctcct ggcagggcgc gcgactcacg gttttgggcg
     2821 ccaggtgcag gatgcggccg taaaagggga ggtgcttgat cgcgtccacg aagcccttgg
     2881 gcggttccgg gtgctggagg aacgcgagga acttgccgat ctcttcgagc ccggcttcgt
     2941 cggtctccag tcccatggcc acgcgtttga ccgtgccgaa caggttggcg agcaggggaa
     3001 tcgacgagcc cttgacgcgt tcgaacagga ccgccgggcc ctcgcgggcc aggaggcggt
     3061 tgagaatttc cgtgacctcg aggcgcgggt ccacctcggc tgtgacgcgg accaattcgc
     3121 ccgctttctc cagggcatcc agaaattcgc gaagatcggt gtacggcatg tgtcggttac
     3181 ctgatggaaa gatctacgat gatcccgagc aagatcacga cgccgatcca cgcgtgggat
     3241 cgaaacagtg cgaagatcgc ggcgcggtcg agtggttgtc gcagtcgcca ggtctgatac
     3301 ccgaacgcgg cggccaccgc ggcgatcgtg gcgacgtaga ccggacccag cggcgcgatc
     3361 acgcccaaga cgatcatccc gatcacggcc aggccgagca gcccgcccac cccggcccac
     3421 gcgtagcggc cgaagaaaat cgcggacgag cgcacgccga tcttctcgtc gtcgtcgcgg
     3481 tccatcaggg cgtagatcgt gtcgtagccc gcggcccaac agaccgtggc gaggaagatc
     3541 acgatcgcgg gcgtgccgac ctcgttgcgc acggcggccc aggcgagcaa cgcgccccat
     3601 ccgaaggccg cgcccatcac ggcctgcggc acggcgatga cgcgcttcat gagcgggtag
     3661 accgcggcga gcgcgaagcc ggccacgctc agcgcgacgg ccaggggatt gagcgcgagc
     3721 gtcagcagca gggcgatgac cgacagcccc gcgcccacgg ccagggcctc cgccgcggtc
     3781 aaacggcccg aggcgagcgg ccgatccttg gtccggtcca cgcggccgtc gaggtgtcga
     3841 tccgccacgt cgttcaacac g
//
LOCUS       DBNGTT010000270         2764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2485500, whole genome shotgun sequence.
ACCESSION   DBNGTT010000270 DBNGTT010000000
VERSION     DBNGTT010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2485500"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2764
                     /locus_tag="AC16BC_06000"
     CDS             <1..>2764
                     /locus_tag="AC16BC_06000"
                     /inference="COORDINATES: protein motif:HMM:NF019325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HIR3241208.1"
                     /translation="TLKGAQQVGGGWGLDLEKTFPDEVVDTLAALEALTTAGAADPTT
                     VARALGYLLDAQNADGGWGAARGMPSEGFYTAHALPVLQGFQQTFQLTATTQAATNLL
                     LSRQNIDGSFGSPSGTAFETAVALQAMLRYPVEAAKSIAAMNYLLSTQLANGSWVDDA
                     YSTALAVRALAEVRPNLSIVTPAVTSTPLNPQEDESASLQATIHNTGIRDASNVVVRF
                     FLGDPAAGGVQIGTDQILGAILSGGSATASITHSFTGTGGRTIFVQVDPSNAIAETSE
                     TDNLASSRLWVATPPDLAVFTADLVPSTFTPEPGTSFALEYTVRNLGEASTGPLTVAV
                     YDGNPGAGGVLLTTQELSGVSGAGSRTSTVGITLTSAVAHTLYVVADSTNQVTEQSET
                     NNQASVTVQVGATPLAADLAVTPMDITLSPARPQSGESVQVTARFRNEGTEPASNVTV
                     ELFDGAPESGGALLASETRTLAPGEEQTLTATWIATAGIHDLYVVLDRTNQLVEIKED
                     NNRASLRVMPDLVDLLVSATDLAFAPSRPVIGDSVTFTATVRNLGIGEAGAFNVALYD
                     GDPAAGGVLLQTYPVPNLPGDANTTVTYTFTGEARTYRFYVVADVDGQVVELDEANNQ
                     AMRSLRIKAPGEILGPDLIPLKIDVDGATTDGQTLAVSGSVQVTVQNTGDAKITTPSS
                     VLVFDDTDFDSHYTAGVDTLLGAADATQTLWPESATLVTVPLSGAVRFLDVPLHVLVD
                     ANDALAETDETNNHLRSGEDCEVRPSQPIQPVMKWRWRGTVGNDFGSSGITMPPAIVN
                     LTDDNGDGRIDNGDIPDLSFIASTDTQGRGMVRALRGDTGAQLFAVRDPNHAPYYIGS
                     LASGDLDGDGRVEFVMSKTGTAGLLAFEHDGSLKWDNAALVTAWNQAHPFQPATMNAY
                     GAPLLAD"
BASE COUNT          509 a          891 c          892 g          472 t
ORIGIN      
        1 acgttgaaag gcgcccagca agtcggggga gggtgggggc tcgatttgga gaagaccttc
       61 cccgatgaag tcgtggacac gcttgcggcg ctggaagcct tgaccacggc cggagcggct
      121 gatccgacca cggtggcgag ggctctgggc tatttgttgg atgcgcaaaa cgccgacggc
      181 ggatggggcg cggcgcgagg gatgccgagc gaggggtttt atacggcgca cgcgttgccc
      241 gtgcttcagg gatttcagca gacgtttcaa ctgacggcca ccacccaagc cgccacgaac
      301 ttacttctca gtcgccagaa tattgatggc agcttcgggt ctccctcggg gaccgcgttc
      361 gaaaccgcgg tcgctctcca agcgatgctg cgctatccag tcgaggccgc caagtccatc
      421 gcggcgatga attacttgct ctcgacccaa ttggccaatg gcagttgggt cgatgatgcc
      481 tactcgaccg cgttggcggt tcgcgccttg gctgaagttc ggccgaatct atcgatcgtg
      541 actccggctg tcacctccac gcccctcaat ccacaggaag acgagtccgc ctcgctgcag
      601 gccacgatcc ataacacggg aatccgtgac gcgtccaacg tggtcgtccg cttcttcctg
      661 ggcgatcccg ccgcaggtgg tgtgcagatc gggactgatc aaatccttgg cgcgatccta
      721 tccggtggct ccgcgaccgc ctcgatcact cattccttca ccggcaccgg cggccgcacc
      781 atcttcgtgc aagtcgaccc gagcaacgcc atcgccgaaa cctcggagac ggacaacctg
      841 gcctccagcc gcctgtgggt ggcgacgcca cccgacctcg ccgtgttcac cgcggatctg
      901 gtgccttcga ccttcacccc tgagcccggc acctcctttg ccctcgaata caccgtgcgc
      961 aatctgggcg aagcgagcac gggtcccttg accgtggccg tgtacgacgg caatccgggc
     1021 gcgggcgggg tgttgctgac cacgcaggaa ctctcgggcg tgagcggggc ggggagtcgg
     1081 accagtacgg tgggcatcac gctgacctcg gcggtggcgc acacgctgta cgtcgtggcg
     1141 gattctacca atcaagtgac cgagcagtct gagacgaaca accaggcctc ggtcacggtg
     1201 caggtgggcg ccacgcccct ggccgcggac ctggcggtca cgccgatgga catcacgttg
     1261 agcccggctc ggccgcaaag cggcgagagt gtgcaagtca cggcgcgctt ccgcaatgag
     1321 ggcaccgagc cggcctccaa tgtcacggtc gaactgttcg acggcgcgcc ggaatcgggc
     1381 ggtgctctgc tcgcgagcga gacacgaaca ctggcgccgg gggaagagca gaccctgacg
     1441 gccacctgga tcgccaccgc gggtatccac gacctgtacg tggtgctgga ccgaaccaac
     1501 cagctcgtgg agatcaagga ggacaacaac cgcgcttcgc tgcgcgtaat gcccgatctg
     1561 gtcgatttgc tcgtctcggc cacagacttg gccttcgcgc ccagccgccc ggtcatcggc
     1621 gacagcgtga ccttcaccgc cacggtgcgc aacctgggca ttggcgaggc cggtgcgttc
     1681 aacgtagcgc tctacgacgg cgaccctgca gcaggcggcg tattgctgca aacgtatccc
     1741 gtacccaatt tgccgggcga cgccaatacc acggtgacct acacgttcac cggcgaggcg
     1801 cgcacgtacc gtttttacgt ggtggcggac gtggacggcc aggtggttga actggacgaa
     1861 gccaataacc aggccatgcg ctcgctgcgg atcaaagccc cgggcgagat cctggggcct
     1921 gacctcatcc cgttaaagat cgacgtggac ggcgcgacca cggacggcca gaccctggcc
     1981 gtcagtggca gcgtgcaggt cacggtgcag aacacgggcg acgccaagat caccaccccg
     2041 tcgtcggtcc tggtcttcga tgacacggat ttcgacagcc actacaccgc gggtgtggat
     2101 acgctgctcg gcgcggccga cgcaacccag accctctggc ccgagagcgc cacgctggtc
     2161 accgtgccgc tgtccggggc cgtgcgcttc ctggatgtgc cgctgcacgt gctagtggat
     2221 gccaacgacg ccctggccga aaccgacgag accaataacc acctgcgttc gggcgaagac
     2281 tgcgaagtgc ggccgagtca gccgatccaa ccggtgatga aatggcggtg gcgaggaact
     2341 gttgggaacg acttcggctc ttctgggatc acaatgccgc cggcgatcgt caatctcacg
     2401 gatgataacg gcgatgggcg gatcgacaat ggggacattc ccgacctgtc gttcatcgcc
     2461 agcaccgata ctcagggacg cgggatggtc cgggcattgc gcggcgacac cggcgcgcaa
     2521 ctctttgcgg tgcgcgatcc gaatcatgcg ccgtattaca tcgggtccct ggcctccggg
     2581 gatttagacg gggacgggcg agtcgagttt gtgatgtcga agacggggac ggctggactg
     2641 ctcgcattcg aacacgacgg gagcctcaaa tgggacaatg cggccctggt cacggcctgg
     2701 aatcaagcgc atccgtttca accggccacc atgaatgcgt atggcgcgcc gctgctcgct
     2761 gacc
//
LOCUS       DBNGTT010000271         1758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_698044, whole genome shotgun sequence.
ACCESSION   DBNGTT010000271 DBNGTT010000000
VERSION     DBNGTT010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_698044"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..518
                     /locus_tag="AC16BC_06005"
     CDS             162..518
                     /locus_tag="AC16BC_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3241209.1"
                     /translation="MKYLLLIYLDEHGLSETERQECYAESTQLAHDLKSGGQYLGANP
                     LHPTAMATSVRMRNGKRLVTDGPFAETREQLGGYFLIDAKNLDEAIAVAARIPMARKG
                     TVEVRPVIDVPGLPAE"
     gene            548..910
                     /locus_tag="AC16BC_06010"
     CDS             548..910
                     /locus_tag="AC16BC_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3241210.1"
                     /translation="MKFMLLVHHDEKAFSTLNETTKKQMLAESVDLTHQFHANGQYLS
                     ASPLHPAATSAIVRVRNGKPFVTDGPFIETREQIAGYFLVTAKDRDEAIAMAARVPGA
                     RIGTVEVRPLIEITGLPE"
     gene            1045..1470
                     /locus_tag="AC16BC_06015"
     CDS             1045..1470
                     /locus_tag="AC16BC_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3241211.1"
                     /translation="MRFMVIVKASKDSEAGVMPSEKLLGEMGKFNEELAKAGVLLAGE
                     GLHPSSKGARVKFSGAKRTVIDGPFPETKELIAGFWLWKVKSKQEAIDWLRRCPNPHN
                     EDSEVEIRQVFEAEDFGAEFTPELREQEEHVRAMAAKKQ"
     gene            1547..>1758
                     /locus_tag="AC16BC_06020"
     CDS             1547..>1758
                     /locus_tag="AC16BC_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HIR3241212.1"
                     /translation="MFKIIAVVAVVLIAAVLIYAATKPDTFRVQRSASIKATPEKIFP
                     LINDLHNFNTWNPYAKKDPNAKGSYSG"
BASE COUNT          404 a          501 c          543 g          310 t
ORIGIN      
        1 ccaagaacac atcaagacgg tgaaaaaagc gggcggtacc gtgaaggatc cgcccgtgga
       61 catccctggt gtcggtctgt atgtctcgtt cttcgatacc gagggcaacc gggtctgcat
      121 gctccaacca cgcatgtgac gtagtcggca gaggacgcct gatgaaatac ctgttgctga
      181 tttaccttga cgagcatggc ctgagcgaga ccgagcggca agagtgctac gcggaatcca
      241 cgcagctcgc gcacgatttg aagtccggcg ggcaatatct gggcgccaac ccgttgcacc
      301 cgaccgccat ggcgaccagc gtccggatgc gaaacggaaa acggctggtc accgacggcc
      361 cgttcgctga gacgcgcgaa caactgggcg gatattttct gatcgatgcc aagaatctcg
      421 acgaagcgat cgcggttgca gcacggattc ccatggcgcg caagggcacg gtcgaggtca
      481 ggcccgtgat cgacgtgccg gggctgccgg ccgagtaacg gctaggcatg gtgatggagg
      541 cgcgttcatg aaatttatgt tgctggttca ccacgacgaa aaggccttca gcacactcaa
      601 cgaaaccacg aagaaacaga tgctggctga gtccgtggac ctcacgcacc agttccacgc
      661 caacggccaa tatttgagcg cgtccccact gcacccggcc gcgacatcgg ccatcgtgcg
      721 cgtgcgaaac gggaaaccgt tcgtgaccga cggcccgttc atcgaaacgc gcgagcagat
      781 cgccgggtat ttcctggtca ctgccaagga tcgcgacgag gccattgcca tggccgcccg
      841 tgttcccggg gcgcgcatcg gaaccgtcga ggtccggcca ttgatcgaga tcaccggttt
      901 gccggaatga tgcgcgattg cctgaaccag ataaaaaaag atcggtgaca tgtcgatttc
      961 acggaccctc gttcgacaaa gaggcagaac cgaagcagga atctgaaaat ctaggggtcg
     1021 ctcataaccc acgggaggtg ttgcatgcga ttcatggtga ttgtcaaagc cagcaaggat
     1081 tcggaagccg gcgtcatgcc gagtgagaaa ctccttggcg agatgggcaa gttcaatgaa
     1141 gagctggcaa aggccggggt cctgctcgct ggcgaaggcc tgcacccgag ctcaaaaggc
     1201 gcgcgggtga aattctccgg cgcgaagcgg accgtgatcg acggcccgtt ccccgagacc
     1261 aaggagttga tcgcgggctt ttggctgtgg aaggtcaagt ccaagcaaga ggcgattgat
     1321 tggctcaggc gttgtcccaa tcctcataac gaggacagtg aagtcgagat ccgccaggtg
     1381 ttcgaggcgg aggatttcgg cgccgagttc acgcccgagc tcagggagca agaggagcac
     1441 gtgcgcgcga tggcagccaa gaagcaatag tcatcgcgcg ctctgcgcgt gcgaccagtt
     1501 tcaggtgcca atcgtgtcgc actgaccacc agcagtggag gcgtttatgt tcaagatcat
     1561 tgccgtcgtc gcagtcgtgc tgattgccgc ggtgctcatc tacgccgcaa ccaagcctga
     1621 cacgttccgg gtccagcgct cggcgagcat caaggccacg cccgagaaga ttttcccgct
     1681 gatcaatgac ctgcacaatt tcaacacgtg gaacccgtac gccaagaaag atccgaacgc
     1741 caagggcagt tacagcgg
//
LOCUS       DBNGTT010000272         5630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_570626, whole genome shotgun sequence.
ACCESSION   DBNGTT010000272 DBNGTT010000000
VERSION     DBNGTT010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_570626"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="AC16BC_06025"
     CDS             <1..151
                     /locus_tag="AC16BC_06025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3241213.1"
                     /translation="FLNHPDLRRILLTDDFDGHPLRKDYPTEGKGWRNTFEFLPKGEG
                     PTVIS"
     gene            281..1453
                     /gene="nuoD"
                     /locus_tag="AC16BC_06030"
     CDS             281..1453
                     /gene="nuoD"
                     /locus_tag="AC16BC_06030"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR3241214.1"
                     /translation="MLRTEELLLNMGPQHPSTHGVLRVVLELEGERILKATPHLGYLH
                     RGTEKLAEGKTYQQILTLTDRLDYVCAMTNNFAYVRAVEKLLGVKVPPRAEYVRTIVA
                     EMQRISGHLFWLGTQALDIGAMTVFFYTFREREILIELFEKLCGARLTYSYYSIGGVS
                     HDLSPEIITALREFLKTFPEKITEYNTLLETNRIWLARTKNVAVISADDAVAYGLTGP
                     TLRGSGVNYDIRKAEPYAAYGDVDFDVPLGRNGDTYDRYFIRIEEMRQSARIISQCLD
                     KLPDGPVSIDVPGITFPSKDRVMVDMESLIHHFKMACEGIKPPKGEVYCATEVPKGEL
                     GFYINSDGGNHPYRLKIRSPSYVHLGAFDHMARGYLIADIVTIFGTYDVVMGECDR"
     gene            1574..2095
                     /gene="nuoE"
                     /locus_tag="AC16BC_06035"
     CDS             1574..2095
                     /gene="nuoE"
                     /locus_tag="AC16BC_06035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR3241215.1"
                     /translation="MLSEAAKTRILDLLPRYPDKRSATLAALRIAQEEKGYITDDAMS
                     DIGVLLEQTPVQVYEVATFYTLYNLWPVGKFHIQVCRTLSCALVGAENIVAYLERKLG
                     IKAGETTRDGLFTLKKVECLAGCGAGPMMQINDDYYEYLTTEKIDRILDDLKRDGKSS
                     LASGPFLIPLQLA"
     gene            2102..3406
                     /gene="nuoF"
                     /locus_tag="AC16BC_06040"
     CDS             2102..3406
                     /gene="nuoF"
                     /locus_tag="AC16BC_06040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR3241216.1"
                     /translation="MPKYEPILLKNILTPGYTGDLAAYERAGGYQAIRKVLKEYQPDQ
                     VIELVKKSGLRGRGGAGFPTGTKWSFIPKDPSLPKYLCINADESEPGTFKDRQLIERD
                     PHQMIEGSLIAAYAINAKVVYIYIRGEFAYGAKVLERALSEARAAGYVGKNVCGSGRE
                     IEVWVHRGAGAYICGEETALIESIEGKRGLPRAKPPFPAINGLYQKPTVVNNVETMAN
                     LPHIVNRGAEWYAAIGTPKSTGTRIFSVSGHVKRPGNYEVPMGIPLRELIDEHAGGVR
                     DGHRIKAIIPGGASASFFTPEHLDVKLDFESVAQAGSMLGSGGVTVMDETTCIVWAAY
                     NLMGFFHHESCGKCTPCREGSNWLYTIMGRIEHGEGRPEDLAQLEHLCGNIAGRTVCA
                     FGDAEVAPILSTLKHFRDEYEAHIREKRCPMAEHSVAVGARP"
     gene            3567..>5630
                     /gene="nuoG"
                     /locus_tag="AC16BC_06045"
     CDS             3567..>5630
                     /gene="nuoG"
                     /locus_tag="AC16BC_06045"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR3241217.1"
                     /translation="MTPTTAPAAPATNLVTLTINGQSVSVPKGTLLIEAAKQANVDIP
                     HFCYHPKLKPDANCRMCLVEIEKSPKLQTSCNLPVAEGMVVSSNSPKVNEARAGVLEF
                     ILANHPLDCPICDKGGECQLQDESHAHTPNYSAFRETKRFFEKEYFGPLIDKEMTRCV
                     QCLRCVRYCDEIVDSRALGSRDRGNSLQIGAMVNRDLDCEFCGGCIQICPVGALTSRV
                     AMYDYRPWQLKKIETICPHCADGCQLKVESRDNVALRITSDHGRGRNNGDLCAKGYFG
                     FDAVNREDRLKKPKIRRDNRLLDASWFETSAFVAKNLATVKAKYGGAAIGGIISAQAP
                     NEDLYLFQKFMRLVLASPHVDSTARLGHHNVVAALRDVVGHGRMTVSYEDIDRANAIV
                     AVGADLTETNPILSYRVKQAARSRGAALVTIGRYGANPGAFVSNLVNRATHPLAAAPG
                     HERAVVLGLLKAVVESGKTGAGLPGAYVDRVRQLVGRVSWEDLAARSGAARQAIEAAA
                     AVYANAERAVILFGRDVVRSADAVDTIRLIADLALTAGKLTAPGCGIGSASDESNDQG
                     AVELGAAPEYFPGLSPVSDAAARQAVAGAWKDELPATPGWTMMEMLEQARAGRLKALY
                     LVGEDPLADLPASADVRGALANLELLICQDAFVGPSSEFEHVILPAVCFAEKEASLTN
                     QEGRVQ"
BASE COUNT         1060 a         1725 c         1893 g          952 t
ORIGIN      
        1 atttctcaat cacccggatc tgcgccggat tttgctgacc gacgacttcg acggtcaccc
       61 tctgcgcaag gactatccga ccgagggcaa ggggtggcgc aacacgttcg aatttctccc
      121 caagggagag gggccgacgg ttatctcatg agcatgatcg cgacctccgc acggacccgt
      181 catggctgat acgaccgaat tccgccggcc ggcgtccgcg gaccgcgacg acttcggcgt
      241 gatctaccat cagccggagc cgccgccgga cgatcgcgcg gtgttgcgga ccgaggagct
      301 gctcctcaac atggggccgc agcaccccag cacgcacggc gtgctccgcg tggtgttgga
      361 gttggaaggc gagcgtattc tcaaggccac cccgcacctg gggtacctgc accgcggcac
      421 cgagaagctt gccgagggca agacttatca gcagatcctg accctgaccg accggctgga
      481 ctacgtgtgc gcgatgacca acaacttcgc atacgtccgc gcggtcgaaa aactgctcgg
      541 ggtcaaggtc ccgccgcgcg cggagtacgt gcggaccatc gtggccgaga tgcagcgaat
      601 ctccggccat ctgttctggc tgggcacgca ggcgctcgac atcggcgcca tgaccgtgtt
      661 tttctacacg ttccgcgagc gggagattct catcgaactg ttcgagaaat tgtgcggcgc
      721 gcgcctcacg tacagttatt actcgatcgg cggggtgagc cacgatctga gcccggagat
      781 catcacggcc ctgcgcgagt ttctcaagac gttcccggag aagatcaccg aatacaacac
      841 cttgctggag accaaccgga tctggctggc gcggaccaag aacgtggccg tgatttcggc
      901 cgacgacgcg gtggcctatg ggctgaccgg ccccacgctg cgggggtcgg gcgtgaatta
      961 cgacattcgc aaggccgagc cgtacgcggc ctacggcgac gtggacttcg acgtgccgtt
     1021 gggcaggaac ggcgacacct atgaccggta tttcatccgg atcgaagaga tgcgccagag
     1081 cgcgcggatc atcagccagt gcctggacaa gttgccggac gggccggtgt cgatcgacgt
     1141 gcccggcatc acattcccgt caaaagaccg cgtgatggtg gacatggaaa gcctgatcca
     1201 ccatttcaag atggcgtgcg agggtatcaa gccgcccaag ggcgaggtct attgcgcgac
     1261 cgaggtgccg aagggcgaac tcgggttcta catcaacagc gacgggggca accatcccta
     1321 tcgtctcaag atccgctcgc ccagctacgt gcacctgggc gcgttcgacc acatggccag
     1381 ggggtatctc atcgcggaca tcgtgaccat tttcggcacg tacgacgtgg tgatggggga
     1441 gtgcgaccgg taaaagatgg ttcaagagtt caacagttca agggttcaac ggaacagtcg
     1501 cacgggcaga gccttgttga accccttgaa ccttgaacct tgaacccttg aacgtatttg
     1561 actcgggtga ctgatgttgt ctgaagccgc aaagactcgg attttggacc tgcttccacg
     1621 gtatcccgac aaacggtcgg ccacgttggc cgcgctgcgg atcgcccagg aggagaaggg
     1681 gtacatcacg gacgatgcga tgagcgacat cggagtgctg ctggagcaga cgccggtgca
     1741 ggtgtacgag gtcgcgacgt tttacaccct ctataacttg tggccggtgg gcaagttcca
     1801 catccaagtc tgtcgcacct tgtcgtgcgc cttggtgggc gcggagaaca tcgtggcgta
     1861 tttggaacgc aagttgggca tcaaggccgg cgagaccacc cgtgacggcc tgtttaccct
     1921 gaagaaagtg gaatgtctgg ccggatgcgg ggccgggccc atgatgcaga tcaacgacga
     1981 ctattacgag tatttgacca cggagaagat cgaccggatt ctggacgatc tcaagcggga
     2041 cggcaaatcg tcgctcgcgt cagggccgtt tctgattcca ctccaactcg cgtaaacgac
     2101 cgtgcccaag tacgaaccga ttctgctgaa gaacattctg acgccgggat atacgggcga
     2161 cctcgccgcg tacgagcgcg cgggcggcta ccaggccatt cgcaaggtgc tcaaggagta
     2221 tcagcccgac caggtgatcg aactcgtgaa gaagtccggg ctgcgcggcc gcgggggcgc
     2281 cgggtttccc accggcacca agtggagttt tatcccgaag gacccctcgt tgcccaagta
     2341 cctgtgcatc aacgcggatg agagcgagcc cggcacgttc aaggaccgcc agttgatcga
     2401 acgcgacccg caccagatga tcgaggggtc gctcatcgcg gcctatgcga tcaacgccaa
     2461 ggtcgtctac atctatattc gcggcgagtt cgcgtacggc gccaaggtgc tggaacgcgc
     2521 gctgagcgag gcgcgggccg cgggctacgt agggaagaac gtctgcggga gcgggcgcga
     2581 gatcgaggtc tgggtgcacc gcggcgcggg cgcctatatc tgcggcgagg aaaccgcgtt
     2641 gatcgagtcg atcgaaggca agcgcggtct gccgcgcgcc aagccgccgt ttcccgcgat
     2701 caacgggctc tatcagaaac ccacggtggt caacaacgtg gaaaccatgg ccaatttgcc
     2761 gcacatcgtg aaccgcggcg cggagtggta cgcggcgatc ggcacgccca agagcacggg
     2821 cacgcggatc ttctccgtaa gcggccacgt caagcggccg ggcaactacg aggtgccgat
     2881 ggggattccg ctgcgcgagc tgatcgacga acacgcgggc ggcgtgcgcg acggccaccg
     2941 gatcaaggcc atcatccctg gcggcgcgtc cgcctcgttt ttcacccccg agcatctgga
     3001 cgtgaagttg gacttcgagt ccgtggcgca ggccggctcc atgctggggt cgggcggcgt
     3061 gaccgtgatg gacgagacga cctgcatcgt gtgggccgcg tacaacctga tggggttttt
     3121 ccaccacgag tcctgcggca agtgcacgcc gtgccgcgag ggcagcaact ggctgtatac
     3181 cattatgggg cgcatcgagc acggcgaggg gcggcccgag gatctggcgc aattggaaca
     3241 cctgtgcggc aacatcgcgg gcaggaccgt ctgcgcgttc ggcgacgccg aggtggcgcc
     3301 gattctgagc acgctcaaac atttccgcga cgagtatgag gcgcatatcc gggagaagcg
     3361 gtgtccgatg gccgagcact cggtcgcggt gggagcccgt ccatgatggg ccatctgctg
     3421 cgttgtcggt cggcgttgcg tgctcacgta cgacccagta cgctccgcgc gcaacgctct
     3481 cccgccgcct tgcatctgtc ccatcctgaa cgggctcccg cgggtaagtc atccgcggtt
     3541 cgaaacccca aggcacaacg aacactatga cgccgaccac cgctccagcg gctccagcca
     3601 cgaatctcgt cacgctgacc atcaacgggc agtccgtgag cgtgcccaag ggcacgctcc
     3661 tgatcgaggc cgccaaacag gcgaacgtcg acatcccgca cttttgttat caccccaagt
     3721 tgaagccgga cgccaattgc cgaatgtgcc tggtcgagat cgagaaatcg cccaagctgc
     3781 aaacctcctg caacctgccg gtggccgagg gcatggtggt atcgtccaac tcgcccaagg
     3841 tgaacgaggc gcgggcgggg gtgctggagt tcatcctcgc gaatcatccg ttggattgcc
     3901 cgatctgcga caagggcggc gagtgccagc tccaggacga gtcgcacgcg cacacgccca
     3961 actacagcgc gtttcgcgag accaagcgct ttttcgagaa ggaatacttc ggtccgttga
     4021 tcgacaagga aatgacgcgc tgcgtgcagt gcctgcgttg cgtgcgctat tgcgacgaga
     4081 tcgtggactc gcgcgcgctg ggctcgcgcg accgcggcaa ctcgttgcag atcggcgcga
     4141 tggtgaaccg cgacctggac tgcgagttct gcggcgggtg catccagatc tgcccggtgg
     4201 gcgccctgac cagccgcgtc gccatgtacg actaccgtcc gtggcagctc aagaagatcg
     4261 agacgatctg cccgcactgc gccgacgggt gccagctcaa agtcgagagc cgcgacaacg
     4321 tggcgctccg gatcacgtcg gatcacggcc gcggccgcaa taacggcgat ctgtgcgcca
     4381 aggggtactt cggcttcgac gcggtcaacc gcgaggaccg gctcaagaaa cccaagatcc
     4441 gtcgagacaa ccgcttgctc gacgcgtcgt ggttcgagac cagcgcgttc gtggccaaga
     4501 acctggccac ggtcaaggcc aaatacggcg gggccgcgat cggcgggatc atttcggcgc
     4561 aagcgccgaa cgaagacctc tatctgttcc agaaattcat gcgcttggtg ctggcgagcc
     4621 cgcacgtgga cagcacggcg cggttgggtc atcacaatgt cgtggcggca ttgcgcgacg
     4681 tggtgggcca cggccgcatg accgtgtcgt acgaggacat cgaccgcgcg aacgccatcg
     4741 tggcggtggg cgcggatctg accgagacga atccgattct ttcgtaccgg gtgaagcagg
     4801 cggcgcgttc acgcggcgcc gcgctggtca cgatcgggcg gtacggggcg aaccccggcg
     4861 cgttcgtgag caacctggtg aaccgcgcca cgcacccgtt ggccgccgcg cccggacatg
     4921 agcgcgcggt ggtgctgggc ctgctcaagg cggtggtcga atccgggaag acgggagcgg
     4981 gcttgcccgg cgcgtacgtg gaccgggtac gccaactggt cggtcgggtg tcgtgggaag
     5041 atctggcggc ccggagcggc gcggcgcgtc aggcgatcga ggccgcggcc gcggtgtacg
     5101 cgaacgcgga gcgcgcggtg atcctgttcg gccgggacgt ggtccgttcc gcggacgcgg
     5161 tggacactat ccgcctgatc gcggacctcg cgctgacggc cgggaagctg accgcgccgg
     5221 ggtgcggcat cgggtccgcg agcgacgagt ccaatgatca gggtgcggtg gagctgggcg
     5281 ccgcgccgga atacttcccc gggttgtcgc ccgtctccga cgcggcggcg cgacaggccg
     5341 tggcgggcgc gtggaaggac gaactgcccg ccacgccggg gtggaccatg atggagatgt
     5401 tggagcaggc gcgcgccgga cggctcaagg ccctgtatct ggtcggggaa gatcccctgg
     5461 ccgatttgcc ggcctcggcg gacgtgcgcg gcgcgctcgc caacctcgag ttgctgatct
     5521 gtcaggacgc gtttgtcggg ccgtcttcgg agttcgagca cgtgatcctg ccggcggtgt
     5581 gtttcgcgga gaaggaagcg agcttgacca atcaggaagg ccgggtccag
//
LOCUS       DBNGTT010000273         3090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2742640, whole genome shotgun sequence.
ACCESSION   DBNGTT010000273 DBNGTT010000000
VERSION     DBNGTT010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2742640"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="AC16BC_06050"
     CDS             complement(<1..589)
                     /locus_tag="AC16BC_06050"
                     /inference="COORDINATES: protein motif:HMM:NF017946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241218.1"
                     /translation="MRKYLTVFRVNWLNSLQYRAPTIIYIVGYTLTVSVLLFLWTAIY
                     RDGGRIGEYALSELVTYYLLQFVINTVVISYVSWEIIDHIREGFFSHFLVKPIDYLTY
                     WLTMNASGKLFETAVVAMVVGGLWAAVGPMVRLPEHPTTYLAFAAALALGVLLAFELE
                     FCIGLIAFWLVQVRVFKYMLQYAMFFFAGTLLPLDV"
     gene            683..2734
                     /gene="prsK"
                     /locus_tag="AC16BC_06055"
     CDS             683..2734
                     /gene="prsK"
                     /locus_tag="AC16BC_06055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HIR3241219.1"
                     /translation="MWWSVLPTVSALWALGLALMVLARNRRSRMHQSFALGMLALSVM
                     EVGHFMAVIQADDEVWWTRVALAGQILLPLPWLMFSLTFGRPDPLEQLRRWRAALAFV
                     AAASLAFLGLLVFKRHLAEDLSLRPVGLWFNAFLLLSLTLVLVNLERTVRSADRGLRW
                     RMKYLILAVFAIMAVAIFDVSQVILYESAERWIAPLFSSVMILGCGLMTFAVVRYRLF
                     EVDIFLSRYVVYNSLTLILVGVYLLGVGLAAQAVKSLGGDSSAYWSALVVVVSVIALA
                     VVLLSYDVRTRMKVFINRHFFKYKYDYRKEWMDLTERLSSKPTVEAIASALSAMLFDT
                     FWIRDTYLWLADERERELAPAEAHPETEALHWTTSALAALKARDEPLVVAAPAEQRLA
                     LPDGAPAEALRALGIRILVPLVVQDRLVGVVGLGAPHTASPLTEEDFDLLKTIGKQVA
                     ASVFNAQLLHELVASKELESFHAFSTFLLHDLKNFVSMLSLLVDNMERNFDSPAFRQD
                     ALKNLSQTVDKMKRLMERLRALAQSPAPAFEPVDISQIGRAVVHEVQPSLNATVLTEW
                     GTVPPVRADAAQIRRVLINLLLNAEEAVGGRGEIHVSTRADNGMVTCRVADNGPGITP
                     EFLQTRLFKPFATTKSGGFGVGLYQCKTIIEAHGGRVAAESRVGQGSILSFSIPATKD
                     M"
     gene            2734..>3090
                     /locus_tag="AC16BC_06060"
     CDS             2734..>3090
                     /locus_tag="AC16BC_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3241220.1"
                     /translation="MSKAKLLVVDDEEAIRNQMRWALADDYEVLLAEDRPSALAQMKQ
                     ERPEVVLLDLGLPPAPRDATEGLQALSELLAADRQAKVIVITGNHDRANALRAVEQGA
                     FDFFSKPADLQDVRVVL"
BASE COUNT          572 a          993 c          945 g          580 t
ORIGIN      
        1 acacgtcgag cggcagcaac gtgccggcaa agaagaacat ggcgtactgc aacatgtatt
       61 tgaacacgcg cacctgcacg agccagaacg cgatcagccc gatacaaaat tccagctcaa
      121 aggccaacag cacacccaat gccagcgccg cggcgaaggc cagatacgtc gtggggtgtt
      181 cggggagtcg caccatggga cccaccgccg cccacaatcc gcccacgacc atcgcgacca
      241 ccgcggtttc aaacagtttt cccgacgcgt tcatggtcaa ccaatacgtc aggtagtcga
      301 tcggtttgac cagaaaatgc gagaagaacc cttcgcgaat atggtcgatg atctcccaac
      361 tcacgtagga gatcaccacc gtgttgatca cgaattgcag gaggtaatac gtcacgagct
      421 cggacaaggc gtactccccg atccgaccgc cgtcccggta gatcgcggtc cagagaaaca
      481 gcaacaccga cacggtcagc gtatacccga cgatgtagat aatggtggga gcgcggtact
      541 ggagggagtt gagccagttc acccgaaaga ccgtcaggta ttttcgcacc gcgtcattgt
      601 acgggaacgc ccgtccggac gcaaaacgtt atgccggtgt ttgccgcgcc cgccgaaaac
      661 ccttagaatc accaccatgt ctttgtggtg gtccgtcctc cccacggtca gcgcgctctg
      721 ggccttgggg ctcgcgctga tggtgttggc ccgcaaccgc cgaagtcgca tgcaccagag
      781 ttttgcgctc ggcatgctcg cgctatcggt catggaggtg ggacacttta tggccgttat
      841 tcaggccgat gacgaggtgt ggtggacccg cgtcgcgctt gccggacaaa tcctcctgcc
      901 ccttccctgg ctgatgttca gcctgacctt cggacgcccc gatcccctgg aacagctgcg
      961 acgctggcgc gcggccttgg ccttcgtggc cgccgcatca ctggctttcc tgggtctgct
     1021 cgttttcaaa cgccatctcg cggaggatct ctcccttcga ccggtcggct tgtggttcaa
     1081 cgcgttcctc ctgctgtcgc tcaccctggt gctcgtcaac ctcgagcgga ccgtgcgatc
     1141 ggccgatcgg ggcctgcgct ggcgcatgaa atacctgatc ctcgccgtgt tcgcgatcat
     1201 ggcggtcgcc atcttcgacg tcagccaggt catcctctac gagtcggccg agcgatggat
     1261 cgctccgttg ttttcgtccg tgatgatcct cggctgcggg ttgatgacct tcgccgtggt
     1321 tcgctaccgc ctctttgagg tcgacatctt cctctcccgg tacgtggttt acaactcgct
     1381 cacgctgatc ctggtcggcg tgtacctcct cggcgtcggg ctcgcggcgc aggccgtcaa
     1441 aagcctcggc ggcgacagca gcgcgtattg gagcgccctg gtggtcgtcg tcagcgtgat
     1501 cgcgctggcg gtcgtgctct tgtcctatga cgtgcgcacg cgcatgaagg tgttcatcaa
     1561 ccgacacttc ttcaagtaca agtacgacta ccgcaaagaa tggatggact tgaccgagcg
     1621 cctgagctcc aaacccacgg tggaggccat tgcgtcggcg ctctcggcca tgttgttcga
     1681 cacattctgg atccgcgaca cgtatctgtg gctggccgac gaacgcgagc gggagttggc
     1741 ccccgcggag gcgcacccgg aaacggaggc gctccactgg acgacgtcgg ccctcgccgc
     1801 gctcaaggct cgcgatgaac cgctcgtggt ggccgccccc gcggaacaac ggcttgcgct
     1861 cccggatggc gcccccgcgg aggcgctgcg ggcgctcggc atccggatct tagtcccgct
     1921 ggtcgtgcag gatcgactgg tcggcgtggt gggattgggc gcgccccata ccgcgtcgcc
     1981 gctgaccgaa gaagacttcg atcttctaaa aaccatcggg aagcaggtgg cggcaagcgt
     2041 gttcaacgcc caactcctgc acgaactggt cgcgtcgaag gagttggaaa gttttcacgc
     2101 gttttccacg ttcctgctgc acgacctgaa gaatttcgtg tccatgctct cgctcctggt
     2161 ggacaacatg gaacggaact tcgacagccc ggcgttccgt caggacgcgc tcaagaattt
     2221 gtcccagacc gtggacaaga tgaagcgact gatggagcgc cttcgggccc tggcgcagtc
     2281 gcccgctccg gccttcgagc ccgtcgacat cagccagatc ggacgcgccg tggtccacga
     2341 agttcagccg tcgctcaacg ccacggtgct gaccgagtgg ggcacggttc cgcccgtgcg
     2401 ggccgacgcc gcgcagattc gtcgggtctt gatcaatctg ctcctcaacg ccgaagaggc
     2461 ggtcggcggg cgcggagaga tccacgtgtc cacgcgcgcg gacaacggca tggtcacctg
     2521 ccgcgtcgcg gacaacggcc ccggcatcac cccggaattt ctccaaacgc gcctgttcaa
     2581 gccgtttgcc accaccaaga gcggcggatt cggggtaggg ctgtatcaat gcaagacgat
     2641 catcgaagcg cacggcggac gggtcgcggc cgagagtcgg gtgggccaag ggagtatact
     2701 gtcgttctcg atccccgcga caaaggacat gtgatgagca aagccaagct cctggtggtg
     2761 gacgatgaag aagccattcg caatcagatg cggtgggcgc tggccgacga ctacgaggtc
     2821 ctccttgccg aagatcggcc ctcggctctc gcccagatga agcaggaacg accggaggtg
     2881 gtgctgctgg atttgggcct tccgccggct ccgcgcgacg cgaccgaagg actccaagcg
     2941 ctcagcgagt tgctcgcggc cgaccggcag gccaaagtca tcgtcatcac cggcaaccac
     3001 gaccgcgcca acgcgttgcg ggccgtggag cagggcgcgt tcgacttctt ctccaaaccc
     3061 gcggatctcc aggacgtccg cgtggtgctc
//
LOCUS       DBNGTT010000274         2331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3125479, whole genome shotgun sequence.
ACCESSION   DBNGTT010000274 DBNGTT010000000
VERSION     DBNGTT010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3125479"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="AC16BC_06065"
     CDS             complement(<1..323)
                     /locus_tag="AC16BC_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HIR3241221.1"
                     /translation="MILGIPIPFLLFAATLLGVAVWHRRSFEVAITGLAVIVAHAVLT
                     TDLNLAAHLAHEARLVANLLGLLLGFAVLAKQFEDSRVPEWLPRWLPEDWTGGSALLV
                     LVAALS"
     gene            621..821
                     /locus_tag="AC16BC_06070"
     CDS             621..821
                     /locus_tag="AC16BC_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3241222.1"
                     /translation="MAKGKVKWFNGSKGYGFISQDEGKDVFVHFSAIQGDGFKSLDEG
                     QEVEFEVVNGPKGLQAANVVKV"
     gene            922..1533
                     /locus_tag="AC16BC_06075"
     CDS             922..1533
                     /locus_tag="AC16BC_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR3241223.1"
                     /translation="MGVLALDVVLIPDAELLSAAITLNRALQGVPDAPIVLDQATCLP
                     HVSLAMGCVREEEISAIANVLDALAAAHPPIELAPTGFHVRQSRTGYLVASMELARTA
                     PLQALHETVMRGVDPLLSRDAAPEMFVAPESISSSTIQWVNAYDSAASFDRFWPHITL
                     GMGALPDDAIPPAPGLASRLALCQLGPHCTCRRVLVEAALRSG"
     gene            complement(1530..1928)
                     /locus_tag="AC16BC_06080"
     CDS             complement(1530..1928)
                     /locus_tag="AC16BC_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR3241224.1"
                     /translation="MPTFYAVLADVTLITHALFVAFVICGQAAVLVGWVRDWRWPRRA
                     TFRGAHLVAIGGVVLKSWLGVPCVLTVLENALRARSGAAPYQETFIAHWVGRLLFYPA
                     PPWVFTLAYSLFALLTLATLIAYPPTRSPR"
     gene            complement(1977..>2331)
                     /locus_tag="AC16BC_06085"
     CDS             complement(1977..>2331)
                     /locus_tag="AC16BC_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3241225.1"
                     /translation="EEHRMTGLSLHPMKEIRIVIQGEHIKFATDLLDGIKATGYTIFH
                     NISGKGHHGFHTAHPMFNDMDSLVMLSTVVPHDKVEPILAGLKPIFDRYTGVMFVSDV
                     AVSRAEYFSGVSGQK"
BASE COUNT          432 a          729 c          769 g          401 t
ORIGIN      
        1 gagagcgcgg ccaccaggac cagcaacgcc gacccgccgg tccaatcttc gggcagccac
       61 cgcggcaacc actccggcac ccgcgagtcc tcgaattgct tcgccaggac cgcaaacccc
      121 agcagcagcc cgagcagatt ggccacgagc cgcgcctcgt gcgccaggtg cgcggccagg
      181 ttcaggtcgg tggtgaggac ggcatgcgcc acgatcaccg cgagcccggt gatcgcgacc
      241 tcaaaactgc gtcgatgcca cacggccacg cccagcagcg tcgcggcgaa caggagaaac
      301 gggatcggta ttccgaggat catggggcgg agggagtgtt gccgacgacg tcggagggca
      361 tgcgcgtggt tctacctgag ccggagcgaa aggtcaaact ccgagcgcac ggcaacagcg
      421 tcgtgaaatc gccgttgacg cagcccgtgt cttcgtggta taaatttatt agtgtccgtt
      481 ggggtggtcg caggtcgtga gtggaacgtt accgcagccg tcaacgcgat gcgctgcggt
      541 ttttttttcg cctcgcaaca cattccgatc gatacgaacg ggttcagtcc cgaattcttt
      601 tggaaggggg tgattggtac atggcgaagg gcaaagtgaa gtggttcaac ggcagcaaag
      661 gctatggctt catcagtcag gatgagggca aagatgtctt tgtgcacttt tccgccattc
      721 aaggcgatgg gttcaagtct ctggacgaag gtcaagaggt cgaatttgag gtcgtgaatg
      781 ggccgaaagg gctccaagca gcgaatgtgg tgaaggtcta accttcccgc gtcgtacacc
      841 acgaagggcc cggggacact ccctccgggc cttttgtatt ttcatccgcg gccgcgcggg
      901 cgcggatgag aggcgttgac catgggtgtg ttggccctcg acgttgtcct gatccccgac
      961 gcggaacttc tctccgccgc gatcaccctc aatcgcgcgc tccaaggcgt cccggacgcg
     1021 ccgatcgttc tcgatcaggc gacctgtctc ccccacgtgt cattggccat gggctgcgtg
     1081 cgcgaggagg agatctccgc gatcgcgaac gtgctcgatg cgctggccgc cgcgcatccg
     1141 ccgatcgagc tcgctcccac cgggtttcac gtccggcaat cccggaccgg gtacctggtg
     1201 gccagcatgg aactggcgcg cacggcaccc cttcaggcgc tgcacgaaac cgtgatgcgc
     1261 ggggtggacc cgttgctgag ccgcgacgcc gcacccgaga tgttcgtcgc gcccgagtcg
     1321 atctcatcga gcacgatcca gtgggtcaac gcgtatgatt cggccgcgag tttcgaccgg
     1381 ttctggccgc acatcacgct cggcatgggc gcgctgccag atgacgcgat cccgccggct
     1441 cccggcctgg cctcacggtt ggcgctctgc cagctcgggc cgcattgcac gtgccggcgg
     1501 gtcctggtcg aagccgcgct tcgaagcggc taacgagggg accgcgtcgg cgggtaggcg
     1561 atcaatgtgg cgagcgtcag gagggcaaag agcgaatacg ccagggtgaa cacccacggc
     1621 ggcgcggggt aaaacagcag ccggccgacc cagtgggcga tgaaggtttc ctggtagggc
     1681 gcggcgcctg agcgcgccct gagcgcgttt tccagcacgg tcagaacgca cggcacgccg
     1741 agccaggatt tgaggaccac gcccccgatc gcaaccaggt gcgcgccgcg aaaggtcgcg
     1801 cggcggggcc agcgccaatc cctcacccac ccgaccagaa ccgcagcctg cccgcagatc
     1861 acgaacgcca cgaagagcgc gtgggtaatc agcgtgacgt ccgcgaggac cgcgtaaaaa
     1921 gtcggcatcc cgcgtatccc gcaggctcac ggcgacgcgg cgtccgccgc gacccgttac
     1981 ttctggccgg aaaccccgga aaagtactcg gcgcggctca ccgccacgtc cgagacgaac
     2041 atgacgccgg tgtagcggtc gaagatgggc ttcaacccgg ccaggatcgg ctccaccttg
     2101 tcgtgcggca ccacggtcga gagcatcacc agactgtcca tgtcgttgaa catggggtgc
     2161 gcggtgtgaa agccgtggtg gcccttgccc gagatgttgt ggaaaatcgt gtacccggtg
     2221 gccttgatgc cgtccaacag gtcggtggcg aatttgatgt gttcgccctg gatgacgatc
     2281 cggatttctt tcatggggtg tagtgagaga ccggtcatgc ggtgttcctc c
//
LOCUS       DBNGTT010000275         5172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1847801, whole genome shotgun sequence.
ACCESSION   DBNGTT010000275 DBNGTT010000000
VERSION     DBNGTT010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1847801"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..812)
                     /locus_tag="AC16BC_06090"
     CDS             complement(405..812)
                     /locus_tag="AC16BC_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241226.1"
                     /translation="MAKGHEVLRQRRAAGLDPAHGGSAAVARGARRVETRRAMTEWEA
                     KHPTPPDQAVYGREVLPRVRSASVPAIVRATGLSYSYCALIRRGLRVPHPRHWDALGK
                     IGGDRYAHEWTPSTPKDDVLGSARFKTHQTGTL"
     gene            complement(796..1419)
                     /locus_tag="AC16BC_06095"
     CDS             complement(796..1419)
                     /locus_tag="AC16BC_06095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HIR3241227.1"
                     /translation="MTGSPRKAVTPAHALLNYLYAIVEAEARLAALAVGVIRGLGLLH
                     ADLRSRDSLACDLMEPVRPLVDRYVWETLSGQAFRREDFFETREGVCRVLPPLTTLLA
                     QTASRWAKAVAPVAERVAQQLVDRTASGEASQRDRRSFTLPTPLTEAKRQAGRRALRE
                     RAGRCGHCASGTVGHRLPDVWRRTHVREACGLRRLRPSHGGGHGQGA"
     gene            1914..4412
                     /locus_tag="AC16BC_06100"
     CDS             1914..4412
                     /locus_tag="AC16BC_06100"
                     /inference="COORDINATES: protein motif:HMM:NF052065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7948 domain-containing protein"
                     /protein_id="HIR3241228.1"
                     /translation="MALAKPMAAEAPSEIAGRYGQLPLSFEVNAGQSDPRARFISRGR
                     GYTIFLTPQEAVIGLLKEGDKGEAGQRVVVRMALLHASKAPTMVGMEPLPGRSNYFVG
                     SDRAKWLTHIPHYAKVKYDDVYPGIDLAYYGAQQRLEYDFEVAPGSDPRAIALDFQGV
                     ERLSIDQQGDLILHTATGELRQLRPVIYQEIEGKRELVSGRYELRGPRHVGFLIGQYD
                     ASKPLVIDPVLSYSTYLGGSGNDEGASVAVDSNGNAYLTGRTFSTNFPTQAPFDPAAN
                     GYADAFVTKLSASGTTLIYSTYLGGNGDDAGNGVAVDSSGSAYVTGTTVAWNFPVVNA
                     YQPTRGTGYGASDAFVTKLAPAGDALVYSTYLGSSSGYDYRGNPNFGGVDQGIGIAVD
                     SSGSAYVTGWTSSARTNGGVAFPHTTGAIDYGCFGNANSGTDGFVTKFSPAGNTLAYS
                     GTLGACTHSSALFGIAVDSSGSAYVTGYTRATNYPVVNPFQSTKAGSAGSYDAVVTKV
                     SPAGNALVYSTYLGGVGVNDIGRAIAVDSVGSAYVAGYTNSMDFPVTSSAWDTTCGTD
                     GTCNSALLDAFVTKFAPAGNTLVYSTYLGAEGNDDAYGIAVDSQGAAIVVGSTASLAF
                     PIVTAFQSGLSGSTDAYVTKVGPIGNTLPYSSYLGGGAADAGVGVAVDSTGNAYIVGR
                     TSSGDFPTQNPYQLVYAGGGDAFVTKVSGPEVTAMVQIEPETVNKDSSGVPINVFISV
                     AGPHVAEDIQIGDGFSVDMAFPTPVPPTCTTPVLPHTAGTEIFTGNNQLNVKFDRRTV
                     EDCVNVGIDIVLTVNGKFTDQHTFSGYDTIRVIE"
     gene            4645..>5172
                     /locus_tag="AC16BC_06105"
     CDS             4645..>5172
                     /locus_tag="AC16BC_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3241229.1"
                     /translation="MASTLLKRREGDVANGRAPAVQAERTTFDDLAALYLADYENNGR
                     RTLDTAEDRVATLKKTFRGIRASHIGTAQIAAHVARRRGEGRAPATINRELSALKRMF
                     SLGARAQPRLVEAIPYIPMLKGEKVRQGFFEEEDYRALLKVLPEHVQIALAIGFWTGL
                     RQGEILNLRWEQVDLN"
BASE COUNT         1054 a         1615 c         1518 g          985 t
ORIGIN      
        1 actgtgccca cctctgaagt cccttgaata ccaaccaacc tccgacggcc cagccaaaga
       61 cttcgactcc agtcaatgag atcaaaaaaa agaggaactg ctcaatccta ccgatactcg
      121 ctgagagatc taaccgtgtc ctttcaaaaa tatcggggat tccgccacta cttttaagcg
      181 actgaatcca tgccgtaatt actaggccca gtacaagcag aacacctata ccaagggcaa
      241 cacttggtcc aaagtatgat agtggtggca aacggaagtc gttactcatc gctgctaagc
      301 aactcgtcta gtcgttaact cccaggcgac aacgcgccat ggccatatct atttgttttt
      361 ggtcccctcc agccaatccg tagaagcctc ggtagatcat cccctcaaag agtccccgtc
      421 tgatgggttt tgaaccgcgc cgatcccagc acgtcatcct tcggagtgga aggggtccac
      481 tcgtgtgcgt aacggtcgcc tccgatctta cccagcgcgt cccagtgcct cggatgcggc
      541 acccgcagcc cgcgccggat gagcgcacag taggagtacg aaagccccgt cgcccgcaca
      601 atcgctggaa cggacgcact cctcacgcgc ggcagcacct cccgaccata gaccgcctga
      661 tcgggtggtg tcggatgctt ggcctcccac tccgtcatcg cgcgccgagt ctcgacccgt
      721 cgggctccgc gcgccacggc cgcgctgccc ccgtgcgcgg ggtccaatcc cgccgcgcgc
      781 cgctgccgca acacctcatg ccccttggcc atgacctccg ccgtgcgagg ggcgcaggcg
      841 tcgcagaccg cacgcctcgc ggacgtgagt gcggcgccac acgtccggca gtctgtgacc
      901 aaccgtcccc gatgcacagt gcccgcatcg gcccgcccgc tcccggaggg ccctccggcc
      961 cgcctgccgt ttcgcttcgg ttaagggagt cggcagcgtg aacgaccggc gatcacgctg
     1021 cgacgcctcg cccgaggccg tgcggtccac gagttgctgg gcgacccgct cggccacggg
     1081 ggccacggcc ttggcccacc gcgaggcggt ctgggccagg agggtggtca acggcggcag
     1141 gacccggcac accccctccc gcgtctcgaa aaagtcctcg cggcggaacg cctggcccga
     1201 cagcgtttcc cacacatacc gatcgacgag gggccggacc ggctccatca ggtcgcaggc
     1261 cagcgagtcg cgggaccgaa gatccgcgtg cagcaggccg aggccccgga tcacacccac
     1321 cgcgagcgcg gcaagacgag cctccgcctc gacaatggcg tacaggtagt tcaacagcgc
     1381 atgggccgga gtgaccgcct tgcggggaga gcccgtcaag accgagcccc gcgcgccgac
     1441 cgtgcgccag tgcgccggga ctcggggctc atccccaaag cgctctggcg cgatacggga
     1501 tggactaaaa caattcattc cattcctgtt gttccagtgt atgcctcagc aattgacaga
     1561 aaaacggttt ttctatacta tcgatcgccc ccacggtccg gtacgtgatt ctgttcgagt
     1621 ctgtcgagca ggaggcagcg cctttgtgtg tgatgaggtc tctaccatta ctgccgccgg
     1681 ttcatcaaac cgttactgaa agtgcaccaa ctttccggct caaactacac gggagatccc
     1741 aagccctagc tctgttatca cggcattcgc tttgcagctg ggacaatgca tggctcgtgg
     1801 cccagacacg agcagcattc cacctttcat actttcatag aatggaggag cgcgatatga
     1861 catcagtaaa cgcggtgttg ctagctttct tagtcctatg gtatccaggc gcaatggctc
     1921 ttgccaaacc tatggccgcg gaagcgccaa gcgaaattgc cggtcgttac ggccaattac
     1981 ccttgagttt tgaagtcaat gccggccaga gtgatcccag agcccggttt atctcacgag
     2041 ggcggggtta tacgatcttt ctaacaccgc aagaagctgt catcggtctt ctcaaggagg
     2101 gggacaaggg cgaggctgga caacgcgtcg tcgtacgtat ggccctgctc catgcatcaa
     2161 aagcgccgac gatggttggg atggagccgc tgccgggacg cagcaactat tttgtgggga
     2221 gcgaccgggc gaaatggctc acccacatcc cacactacgc caaggtgaag tacgatgacg
     2281 tctatccggg catcgatctg gcctattacg gggcgcagca gcgcctcgaa tacgacttcg
     2341 aggtcgcccc gggtagcgat cctcgtgcga tcgcgctgga tttccagggc gtcgaaagac
     2401 tgagcatcga tcagcagggc gatctcattc tgcacaccgc aaccggtgag ttgcgccaac
     2461 ttcggccggt gatctatcaa gagatcgagg gtaagcggga gttggtttcc ggccgctatg
     2521 agctcagagg gccgcgccac gtcggattcc tcatcggcca gtatgatgcg agcaagcctc
     2581 tggtcatcga ccccgtccta tcctactcga cctacttggg tgggagcggg aacgatgagg
     2641 gggcgtctgt ggccgtggat tccaacggta acgcgtatct gaccggccgg acgttttcga
     2701 caaatttccc gacgcaagcg cctttcgacc cggcggccaa tggctatgcc gacgcgttcg
     2761 tcaccaagct ttcggcctcg gggacaaccc tgatctactc cacctatctc gggggaaacg
     2821 gggacgatgc aggcaatggt gttgccgtcg attcctccgg cagcgcatac gtgaccggaa
     2881 cgaccgtcgc gtggaacttc ccagtggtca acgcctacca accaacccga ggcacgggct
     2941 atggggcttc cgatgccttc gtaaccaagc tggcgcctgc cggtgatgcc ctggtgtact
     3001 ccacctatct gggaagcagc tccggttatg attaccgcgg caatcccaac tttgggggcg
     3061 tcgatcaagg aattggaatc gccgtggaca gcagcgggag cgcctatgtt accggatgga
     3121 cgagcagcgc tcgaaccaac ggtggggtgg cgttccccca taccaccggc gcgattgact
     3181 atggttgctt cggcaatgcc aactccggta ctgatgggtt tgtcaccaag ttctcaccag
     3241 ccgggaacac cctggcctac tccggcactt tgggcgcatg cacccactcc tccgccctgt
     3301 ttggcatcgc tgtggacagc agtggaagcg cctatgtcac cggctacacc cgcgcgacca
     3361 actatccggt ggtgaatcca ttccaatcca caaaggccgg cagcgcagga tcatacgatg
     3421 cggttgtgac caaggtttcg cccgccggta acgcattggt ctactcgacc tatctgggcg
     3481 gcgtcggcgt taacgatatc ggtcgcgcta tagccgtcga cagcgtgggc agcgcatacg
     3541 tggcgggcta cacgaactcc atggacttcc ccgtgacgtc gagtgcctgg gacacaacct
     3601 gcggcaccga tggcacctgt aatagcgccc ttttggacgc attcgtgacg aagttcgctc
     3661 cagctggaaa tacgctggta tattcgacgt acctgggcgc cgaaggaaac gatgacgcct
     3721 acgggatcgc ggttgacagc caaggagctg cgattgtggt ggggtccacc gcctctttgg
     3781 cgttcccgat cgtaaccgcg ttccaatcag ggctcagcgg ctcaacggac gcgtatgtaa
     3841 ccaaggtcgg acccattgga aacacgttgc cgtactcgtc gtatcttgga gggggtgctg
     3901 ccgatgcggg agtcggtgtc gccgttgact cgaccggcaa cgcctacatt gtcggtcgaa
     3961 caagctcggg cgatttcccg actcagaatc cttatcaact tgtatatgcc ggtggaggcg
     4021 acgcgtttgt gaccaaggtg tccggtccag aggtgactgc gatggttcag attgaacccg
     4081 agacagtcaa caaagatagt tcgggggtgc cgatcaatgt gttcatttcg gttgcgggtc
     4141 cccatgtggc cgaggacatc cagatcggcg acggcttttc ggtcgacatg gcattcccaa
     4201 ctccggtgcc tcccacgtgt accacgccag tccttccgca cacagctggc acggagatct
     4261 tcacgggcaa caaccagttg aacgtcaagt tcgaccgccg aacggtcgaa gattgcgtca
     4321 atgtcggcat agatatcgtc ctgacggtga atggaaagtt tacggaccaa cacaccttct
     4381 caggctacga tacgatccgt gtaatcgagt aagcacagtc ccatccggac ccgtacagag
     4441 gggcggctaa ccagccgccc ctttttcctg tgaggcacac acttgaaacc gaattaaaaa
     4501 caaacaacgg ttattaacat atctcagggc atcggttgga tcttcctact ttttggctga
     4561 cacccacccg gtcagtaccc taagaaggcg tcccctctgg gggagtgatc gcttagggag
     4621 tcgtcggggt cggacctcaa gtccgtggcc tcgacgctgc tgaaacgccg cgaaggcgac
     4681 gtggcgaacg gccgggctcc ggcggttcag gcggaacgca ccacgttcga cgacctggcc
     4741 gcgctgtatc tcgcggacta cgagaacaac gggcgacgca ccctcgacac cgcggaagac
     4801 cgggtggcca cgctgaagaa gacgtttcgg gggatacggg cgtctcacat cgggacggcc
     4861 cagattgcgg cgcatgtggc ccggcgacgg ggtgagggcc gcgcgcccgc cacgattaac
     4921 cgggaactca gcgcgctcaa gcgcatgttc tccctcggcg cgcgggcgca gccccgcttg
     4981 gtcgaggcga tcccgtatat cccgatgctc aagggcgaga aggtccggca aggctttttc
     5041 gaggaagagg actaccgcgc gctcctgaag gtattgccgg aacacgtgca aatcgcgctc
     5101 gccatcggat tctggaccgg gctccggcaa ggcgaaattc tcaatctgcg ttgggaacaa
     5161 gtcgatttga ac
//
LOCUS       DBNGTT010000276         4340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1848125, whole genome shotgun sequence.
ACCESSION   DBNGTT010000276 DBNGTT010000000
VERSION     DBNGTT010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1848125"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /gene="rpsC"
                     /locus_tag="AC16BC_06110"
     CDS             complement(<1..393)
                     /gene="rpsC"
                     /locus_tag="AC16BC_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR3241230.1"
                     /translation="MGHKVHPTGFRLGYIKTWNSRWFAGKDYASLLHEDLKVRKIVKA
                     KLYHAGIARIELERSGNQLRINLHTSRPGIIIGRKGAEVDKLKAEIEALTKRQVAINI
                     REIKKPEVDAQLVAENIALQLEKRIAFRR"
     gene            complement(383..781)
                     /gene="rplV"
                     /locus_tag="AC16BC_06115"
     CDS             complement(383..781)
                     /gene="rplV"
                     /locus_tag="AC16BC_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR3241231.1"
                     /translation="MAEAKAVLKFSRIAPRKARNVVDLIRGKRAGEALSALKFTARGA
                     ARVVEKVLRSAIANAENREIGDVDDLVVTRAYVDQGPTLKRIQPAPQGRAHAIHKRMS
                     HITVVVTPKPRKTRRAATAGGAKGAEHRGA"
     gene            complement(842..1129)
                     /gene="rpsS"
                     /locus_tag="AC16BC_06120"
     CDS             complement(842..1129)
                     /gene="rpsS"
                     /locus_tag="AC16BC_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR3241232.1"
                     /translation="MPRSVKKGPFVDASLMAKIEKMNQAREKKIIKTWSRRSTIVPEM
                     LGHTLAVHNGKKFIPVYVTENLVGHKLGEFAPTRLFKGHGSAKTEKAAALK"
     gene            complement(1135..1956)
                     /gene="rplB"
                     /locus_tag="AC16BC_06125"
     CDS             complement(1135..1956)
                     /gene="rplB"
                     /locus_tag="AC16BC_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3241233.1"
                     /translation="MAVRDYKPTSPGRRAMTCSTFEEVTKRSPEKSLLVSLPPSGGRN
                     NKGKMTVRHRGGGHKRAFRVIDFKRDKFDIPAKVAAIEYDPNRSARIALLYYRDGEKR
                     YILAPQGLTVGETIMSGPNAEIRVGNALPLSKITVGTQIHNIEMRPGKGGQLARSAGA
                     VAQLMAKDERYAQVRLGSGEVRLINSQCIATVGQVGNLDHENVSIGKAGRSRWLGRRP
                     KVRGVAMNPVDHPHGGGEGKAGQGNPHPVSPWGMLTKGYKTRRRKDTTRFIIKRK"
     gene            complement(1961..2260)
                     /gene="rplW"
                     /locus_tag="AC16BC_06130"
     CDS             complement(1961..2260)
                     /gene="rplW"
                     /locus_tag="AC16BC_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR3241234.1"
                     /translation="MKTKVDPHEILIAPLLTEKSTRMREAHNQISFMVSARANKSEVK
                     KAVEEALKVKVESVNILNVTGKIKRLGRFEGRRASWKKGIVTLKPDQKLDIFEGG"
     gene            complement(2311..3207)
                     /gene="rplD"
                     /locus_tag="AC16BC_06135"
     CDS             complement(2311..3207)
                     /gene="rplD"
                     /locus_tag="AC16BC_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012782.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR3241235.1"
                     /translation="MSPSKRGDGKDKPKKSAKPGSSASRAKRPAASKTRQAAPKRAAK
                     PKKSAPRAAAKAPKRAASAPKKAATAAPKPKAAAPAPLRPVEVTASGARVVDVLNVKR
                     ESVGRVELNGPLFVSEPNRALLHEAVVMQLASRRQGTADTKERGEVSGTGRMPWKQKG
                     TGRARAGSVRSPLWRKGGIVFGPTPRDYGYAFPRKKGRAALAGAMSAKLAQNEVLVLD
                     QLALAEAKTRLMASVLETLGLDGRVLIVHRDDDGHIRRASANLGRVTVVDVPGLNVYD
                     VLAHQHIVLMQSDLGRLAEVWS"
     gene            complement(3204..3842)
                     /gene="rplC"
                     /locus_tag="AC16BC_06140"
     CDS             complement(3204..3842)
                     /gene="rplC"
                     /locus_tag="AC16BC_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR3241236.1"
                     /translation="MIGGLLGRKLGMTQVFGENGRLIPVTVVEAGPCRVVQLKTKETD
                     GYQAVQLSFGAKREQRVNKPLLGHFKKANVPPTYHLREFPTDGQGVEVGQTVTAETLQ
                     KGEMVSVSGVSKGKGFAGVMKRHHFAGGPATHGSMFHRQPGSIGASSFPSRVFKGKRL
                     PGHMGAERVTVKGLEVVEVRPDENLVLVRGAIPGPTGTVVEIRRVTTAGGVK"
     gene            complement(3839..4153)
                     /gene="rpsJ"
                     /locus_tag="AC16BC_06145"
     CDS             complement(3839..4153)
                     /gene="rpsJ"
                     /locus_tag="AC16BC_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR3241237.1"
                     /translation="MAAANQKIRIRLKGYDYRVLDQSVVEIVETAKRTGAKISGPVPL
                     PTRINRYTVLRSPHVDKKSREQFEIRTHKRLIDIMESSPETVDALMKLNLAAGVHVEI
                     KL"
     gene            complement(4204..>4340)
                     /locus_tag="AC16BC_06150"
     CDS             complement(4204..>4340)
                     /locus_tag="AC16BC_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR3241238.1"
                     /translation="VMPGDNVRMEVDLITPIAMEEGLRFAIREGGRTVGAGVITGIIA
                     "
BASE COUNT          634 a         1529 c         1299 g          878 t
ORIGIN      
        1 gcgccggaac gcgatccgct tctccaactg caacgcaata ttttccgcga cgagttgggc
       61 gtccacctcg ggcttcttga tctcccggat gttgatcgca acctggcgct tggtgagggc
      121 ctcgatctcc gccttgagct tgtcgacctc ggcgcccttg cggccgatga tgatgccggg
      181 gcgggacgtg tgcagattga tccggagctg gttgcccgag cgctccagct cgatccgcgc
      241 gatgcccgcg tgatagagct tggccttcac gatcttgcga accttcagat cctcgtgcag
      301 gagcgaggcg taatccttgc ccgcgaacca gcgcgagttc cacgtcttga tgtagcccaa
      361 ccggaacccg gtcgggtgga ccttatgccc cacggtgttc ggctcccttc gccccgccgg
      421 ccgtcgccgc tcgccgcgtc ttgcgcggtt tgggcgtgac caccacggtg atgtgactca
      481 tgcgtttatg aatcgcgtgc gcgcgacctt gcggcgccgg ctgaatgcgc ttcagagtcg
      541 ggccctggtc cacgtacgcg cgggtgacga ccaaatcgtc cacgtccccg atctcccggt
      601 tctcggcgtt cgcgatcgcg gaacgcagca ccttctccac gacccgcgcg gcgccgcgcg
      661 cggtgaactt gagcgcggag agcgcttcgc ccgcccgctt gccgcggatc agatccacca
      721 cgttgcgcgc cttgcggggc gcgatccgcg aaaatttcag gactgccttt gcttccgcca
      781 tgttgtccga actctcccct ctcacccgac gccccgcatg ccggccggcg cgggcccgcc
      841 cttacttgag cgcggccgcc ttctccgtct tggccgaacc gtggcccttg aacaatcgcg
      901 tgggcgcgaa ctcaccgagc ttgtgcccca ccagattctc ggtcacgtag actggaatga
      961 actttttccc gttgtgcacg gccagcgtgt gccccaacat ttcaggcacg atggtcgacc
     1021 gacgggacca ggtcttgatg atcttttttt cccgtgcctg attcatcttc tcgatcttgg
     1081 ccatcagact ggcgtcgaca aacggccctt tttttaccga acgtggcatc gcgtctactt
     1141 cctcttgatg atgaaccgcg tcgtgtcttt gcgacgcctg gtcttgtagc ccttggtcaa
     1201 catgccccaa ggcgacaccg gatggggatt gccctgtccc gccttgccct cgccgccgcc
     1261 gtgggggtga tccaccggat tcatggccac gccgcgcacc ttgggacgac gacccagcca
     1321 gcgcgagcgc ccggccttgc cgatggacac gttttcgtgg tcgaggttgc ccacctgccc
     1381 cacggtcgcg atgcactggg agttgatcaa tcgcacttcg cccgacccca gacgcacctg
     1441 cgcgtaccgc tcgtccttgg ccatcagttg cgcgaccgcc cccgcgctcc gggccagttg
     1501 cccgcccttg ccggggcgca tttcaatgtt gtggatctgc gtgccgaccg tgatcttgga
     1561 cagcggcagc gcattgccca cccggatctc cgcgttgggg cccgacatga tcgtttcccc
     1621 gaccgtcaag ccctggggcg cgagaatgta ccgcttctcc ccgtcgcgat agtacagcag
     1681 cgcgatccgt gccgaccggt tgggatcgta ttcgatcgcc gcgactttcg ccgggatatc
     1741 gaacttgtcg cgtttgaaat cgatcacgcg gaacgcgcgc ttgtgcccgc cgccccggtg
     1801 ccgaaccgtc atcttcccct tgttgttgcg cccgccggac ggagggagcg acacgagcag
     1861 ggatttctcc ggactccgtt tggtgacctc ctcgaacgtg gagcacgtca tggcccggcg
     1921 gccgggcgag gtcggtttgt aatccctcac ggccatggcc tcagcctccc tcgaagatgt
     1981 cgagtttttg atccggcttc aacgtcacga tgcccttctt ccacgacgcg cgccggccct
     2041 caaatcgccc gagccgtttg atcttcccgg tgacgttgag gatgttcacg gattcgacct
     2101 tcaccttgag cgcttcctca accgccttct tgacttccga cttattggcc cgcgccgaaa
     2161 ccatgaacga gatctggttg tgcgcctcgc gcatccgcgt gctcttctcg gtgagcagcg
     2221 gcgcgatcag aatctcgtgc ggatccacct tggttttcat caccccggcg gccttggggg
     2281 cctcgggttt cgccttacgg gttgccgcgc tcacgaccac acctccgcca accggccaag
     2341 atcggactgc atcagcacga tgtgctggtg cgccaacacg tcgtacacgt tgagccccgg
     2401 cacgtccacg accgtgaccc ggccgaggtt cgcgctggcc cgccggatat gcccgtcgtc
     2461 gtcgcgatgc acgatcaaca cccggccgtc caatcccagc gtctcgagca ccgaggccat
     2521 gaggcgggtc ttcgcctcgg ccaacgccaa ctgatcgagc acgaggactt cgttctgcgc
     2581 gagcttcgcg ctcatggcgc cggccagcgc cgcgcggccc ttcttgcggg ggaacgcgta
     2641 gccgtagtcc ctgggcgtcg gaccaaacac gatcccgccc ttgcgccaca gcggcgaacg
     2701 aaccgagccc gcccgcgccc gacccgtgcc cttctgcttc cagggcatgc gtccggtgcc
     2761 gctcacctcg ccgcgctcct tggtgtccgc ggtgccctgc cgccgcgacg ccaattgcat
     2821 caccaccgcc tcgtgcaaca gcgcgcggtt gggctcggag acgaacaggg gaccgttcaa
     2881 ttccacgcgc ccgaccgatt cgcgcttcac gttcaacacg tcgaccaccc gcgcgcccga
     2941 cgcggtcact tcaaccggcc ggagaggcgc aggagcagcc gccttcggtt tcggcgccgc
     3001 ggtcgcggcc ttcttgggcg cgctcgcggc gcgcttcgga gccttggccg ccgcgcgcgg
     3061 agccgacttc ttgggcttgg cggcgcgctt cggagcggct tggcgcgtct tgctcgccgc
     3121 gggacgtttg gcgcgagacg cggaggaccc gggcttggcg gattttttcg gcttgtcctt
     3181 gccgtcgccg cgcttgctcg ggctcatttc acccccccgg ccgttgtcac acggcggatc
     3241 tcgaccaccg tgccggtggg tcccggaatg gccccgcgca ccagcacgag gttttcgtcg
     3301 ggccgcacct ccaccacttc aagtcctttg acggtcacgc gttcggcgcc catatgtccg
     3361 ggcaggcgtt tgcccttgaa cacgcgcgac ggaaatgagc tggctccgat cgatcccggc
     3421 tggcgatgga acatcgaccc gtgggtcgcg ggtcctccgg caaaatggtg ccgcttcatg
     3481 acgccggcaa accccttgcc cttcgagacg ccgctcaccg agaccatctc gcctttttga
     3541 agcgtctccg cggtgacggt ctgcccgact tccacgccct gaccatcagt cgggaattcc
     3601 cgcaggtggt acgtcggggg cacgttcgcc ttcttgaagt gcccgagcag aggcttgttg
     3661 acccgctgct cgcgcttcgc gccgaaggac aactgcaccg cctggtagcc gtccgtttcc
     3721 ttggtcttga gctgcaccac gcggcagggc ccggcctcga ccacggtgac cgggatgagg
     3781 cggccgtttt ccccgaagac ctgggtcatg ccgagttttc ggccgagtaa gccgccaatc
     3841 ataacttgat ctccacgtgc acgcccgcgg ccaggttgag cttcatcagg gcgtccactg
     3901 tttccggcga ggactccatg atgtcgatca gtcgcttgtg cgtgcggatt tcaaactgct
     3961 cgcgggattt cttgtccacg tgcggcgaac gcaacacggt gtagcgattg atccgggtcg
     4021 gtaacggcac cggcccggaa atcttcgcgc cggtgcgctt cgcggtttca acgatttcca
     4081 ccacggactg gtcgagcacg cggtaatcgt agcctttgag tcgaatacga atcttttggt
     4141 tcgcagcagc catttcctgt tccttcgcgg ggcgaacgaa cgccccttca gtgggtcaac
     4201 gcctcaggcg atgatgccgg tgatgacgcc ggcgcccacg gtgcggccgc cctcgcggat
     4261 ggcaaaccgc agcccctctt ccatcgcgat cggcgtgatc aggtccactt ccatccgcac
     4321 gttgtccccc ggcatcacca
//
LOCUS       DBNGTT010000277         6058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2869735, whole genome shotgun sequence.
ACCESSION   DBNGTT010000277 DBNGTT010000000
VERSION     DBNGTT010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2869735"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="AC16BC_06155"
     CDS             <1..478
                     /locus_tag="AC16BC_06155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3241239.1"
                     /translation="RARGVRARFILVGWSDAGNPSAIPAEQLERWHRQGHVEWCGRRE
                     DMPNVYAQSHVVCLPSYGEGVPKTLIEAAACGRPIVTTDVPGCREIVRHGDNGLLVPV
                     RDSAALAEAIRTLVEDRDLRIRMGERGRTIATEEFSVERVVRETLAVYRDLLGAPS"
     gene            530..1150
                     /locus_tag="AC16BC_06160"
     CDS             530..1150
                     /locus_tag="AC16BC_06160"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3241240.1"
                     /translation="MLKRLVDVGGAVAGLLVLSPVMLVVAVLVYLRMGAPILFLQERP
                     GYKGAPFVLLKFRTMSERRDHAGALLPDEQRVTRLGRFLRRTSLDELPSLVNVLRGDM
                     SLVGPRPLLMEYLDLYPPEFARRHDVKPGLTGWSQIHGRNDMTWERKFALDVWYVDHR
                     GFWLDAGILAATLGTVFRREGITPAGAESTPKYRGPCERKSDEVSV"
     gene            1147..1809
                     /locus_tag="AC16BC_06165"
     CDS             1147..1809
                     /locus_tag="AC16BC_06165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR3241241.1"
                     /translation="MNAMRVVIVGAGGHGHVVADIFLARSRSEAVRVVGFLDDDSSLH
                     GTQIMGIPVIGSFERLGDVEHDGVIVAIGTNRTRGRVFDACKARGERLVNAIHPAAVV
                     GTGARFGEGVMVCAGVVVNPLAVIGDDVILNTSCTVDHHVGIEAHAHVGPGVHLAGEV
                     RVGEGALVGVGANVIPRCAIGAWSVIGAGSAVITDVPAETTAVGVPARVISGVYQVEQ
                     RP"
     gene            1806..3011
                     /locus_tag="AC16BC_06170"
     CDS             1806..3011
                     /locus_tag="AC16BC_06170"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3241242.1"
                     /translation="MMIPMSGPDLTEVEIEAVTSVLRTPCLSIGPRLVEFEERFAAFV
                     GARYGIGVSSGTAGLHLCVIASGVGEGDLVITTPFSFVASANALLYERAIPIFVDVNE
                     ADGNIDGSLVAEAAEDLRRGGRFAQRWLPPSWDISPAGRFAGILPVHVFGQPADMDSL
                     LDTAQRFDVPIIEDACEAVGSAYKGRRVGTFGRAAVFAFYPNKQMTTGEGGMIVTSDP
                     EWNDRFRSLRNQGRDIFDAWLTHSRLGYNYRLSELGAALGVAQVSRLDALLCQRARVA
                     GWYNQRLSGQDAVRIWSVAKTTTTMSWFIYVVRLAEGVARDRVMAELEKRGIPSRPYF
                     SPIHLQPLYVQRFGYKPGRYPVAEALGASCLALPFSGVMTETQVDYVCQNLIEVVHHC
                     GDHRGALAR"
     gene            3264..5180
                     /locus_tag="AC16BC_06175"
     CDS             3264..5180
                     /locus_tag="AC16BC_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR3241243.1"
                     /translation="MSAVFLLELAVATGLAWLVRFEGDVPSGGWDLAWSAFPLIAAVY
                     LIAYVLFGAHRGLWSFTGLHDFVKIPKVALAGAIGSYVLTRDVLGWINYPRSIYILTA
                     VFLFVLMALDRGGVRLLQQQAHRWGKSGIPVVIVGAGQAGEMLVRDMLHAEAGAYRPA
                     AFIDDDASKHGSTIHGVPVVGGHAALAAAIARYQPQEVIIAIPSASPAQLRRIVGACQ
                     PCGLPIKVLPSVHDILRGRVALSSVRPLALADLLTREPVPSDTAGLASLIRGKRVLVT
                     GAGGSIGSELCRQIAQHAPAQLVLVERYENNLHAVAAAIDDLYGAEFVDTRAFLADVL
                     DELRMQRIFEEHRPQLVFHAAAHKHVPIVEENPCEGVLNNVLGTHRVAMLCRRWSVER
                     MILISTDKAVNPTNVMGATKRFAESIVTALSGTGPTRYITVRFGNVLGSNGSVVPRFQ
                     QQIARGGPVTVTHPEIERFFMLIPEAVHLVLEAARRGRGGEVFVLDMGDPIKIVDLAH
                     NMIRLAGFVPNDDIPVVFSGLRPGEKLFEELFEKDERVEPTEHPKLRMAVSDAAWSET
                     ELRVAVTAVTTAARVGDGDGLREALRQFVRSYQPEALPATTHANGSGVNGVPVDVEVS
                     VATVRSEARNRASS"
     gene            5344..5973
                     /locus_tag="AC16BC_06180"
     CDS             5344..5973
                     /locus_tag="AC16BC_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HIR3241244.1"
                     /translation="MTPSSVTYRVRPGPEAFLPPAAASMGIVLPDPGQAHIEGVIVDE
                     AQAFEEAARRFLGAKVPTIFPGPLVLWNWNETSKKKAAAIKKLYDAMKECAPAGSKPM
                     LIPMADYRPKYPKINPEIEINPNHPNLTIWHNRIDVCMFVGVHCHQANLALKIIRGGT
                     NCFTIAMCAQAGHEDANLSFRDASPEKILKLAEAVRRVAAGPSGNGGKP"
BASE COUNT          961 a         1787 c         2116 g         1194 t
ORIGIN      
        1 ccgtgctcgc ggtgttcggg ccagattcat tctggttggt tggagcgacg ccggcaatcc
       61 atcggcgatt cccgccgagc agttggagcg gtggcatcgg cagggccacg tggagtggtg
      121 tggacgccga gaggatatgc ccaacgtgta cgcccagtca cacgtcgtct gtctcccttc
      181 gtacggtgaa ggggtcccaa agacgctcat cgaagccgcg gcgtgcggcc gacccatcgt
      241 aacgacggac gttccaggtt gtcgcgagat cgtccggcac ggcgacaacg gtttgttggt
      301 gccggttcgc gattcggcgg ctcttgcgga ggcgatacgg acgctggtcg aggaccgcga
      361 cctgcggatt cggatgggcg agcgaggtcg aacgatcgcc actgaggagt tttcggtcga
      421 gcgcgtggta cgggagacat tggcggtgta tcgagacctc ctgggcgctc cttcgtgagg
      481 actcgccacc tgcgcacggg aaggcattgc ctgagtatgg ggcgactaga tgcttaagcg
      541 gctcgtcgat gtgggaggcg cggtggcggg actgctggtg ttgtctccgg tgatgcttgt
      601 cgtggccgtc ctcgtctatt tgaggatggg tgcgccgatt ctatttcttc aagagcgccc
      661 cggttacaag ggggctccgt tcgtcttgtt gaagtttcgg acgatgagcg agcgccggga
      721 tcacgcgggc gccttgctgc ctgatgaaca gcgggtgacg cgtctgggcc ggtttctacg
      781 ccggacaagc ctggatgaac tacccagtct tgtgaatgtg ttgcggggag acatgagcct
      841 tgtggggcca cgaccgctgt tgatggaata tctcgacttg tatccgccgg agttcgctcg
      901 gcgtcatgac gtgaaaccgg ggctgaccgg ttggagccag atccacggcc ggaatgacat
      961 gacctgggag cggaagttcg cgctggacgt gtggtacgtt gaccaccgcg gtttctggtt
     1021 ggacgccggg attctcgcgg ccacgctcgg caccgtgttt cgtcgcgaag gcatcacgcc
     1081 agccggggcc gaatccacac cgaagtaccg tgggccgtgt gaacgaaagt ccgatgaggt
     1141 gtcggtatga acgcgatgcg tgtagtaatt gtgggggccg ggggccacgg acatgttgtg
     1201 gcggacattt ttctcgcgcg ctcgaggtct gaggcggttc gcgtcgtcgg gtttcttgac
     1261 gacgacagct cccttcacgg cacccagatc atgggcattc ccgtcatcgg gtcgttcgag
     1321 cgtttgggcg atgtcgagca cgacggcgtg atcgttgcga tcgggaccaa tcgcacgcgg
     1381 ggccgggtct ttgacgcctg taaggcccgc ggggagcggc tcgtgaacgc gattcacccg
     1441 gccgcggtgg tcggaacggg cgctcgcttc ggcgaaggcg tcatggtgtg cgcgggggtc
     1501 gtggtcaatc ccttggcggt gatcggggac gacgtgatcc tgaacacgag ttgtaccgtg
     1561 gaccatcacg ttgggatcga agcccacgcg cacgtcggcc caggggttca cctggcgggc
     1621 gaggtccggg tgggcgaagg ggcgctggtc ggggtaggag ccaacgtgat cccgcgttgt
     1681 gcgatcgggg cgtggagcgt gatcggcgct ggctcggcgg ttattacgga tgtgccggct
     1741 gaaaccacgg ccgtcggcgt cccggcacgg gtgatcagcg gagtctacca ggtggagcag
     1801 cggccatgat gatcccgatg tctgggccgg atctcacgga agtggagatt gaggcggtca
     1861 cgagtgtgtt gcgaacgccg tgtttgagca tcggcccaag gctcgtcgaa tttgaagagc
     1921 gatttgccgc gttcgttggg gcgcggtacg gcattggcgt ctccagcggg accgcaggcc
     1981 ttcacttgtg cgtcatcgcg tcaggcgtcg gtgaaggcga tctggtgatc acgacgccct
     2041 tttcgttcgt cgcgtccgcg aatgcgcttc tctatgagcg ggcgatcccg atcttcgtgg
     2101 atgtcaacga ggccgatggg aacatcgacg gctcgctcgt ggccgaggcc gctgaggatc
     2161 tccgacgggg aggtcgcttc gctcagcggt ggcttccgcc gagctgggac atttcgcctg
     2221 cgggccgatt cgccgggatc ttgccggtcc atgtgttcgg tcaaccagcc gatatggatt
     2281 cccttctcga tacggcgcaa cgtttcgacg ttccgatcat cgaggacgcg tgcgaagccg
     2341 tcggatcagc atataaggga cgccgtgtcg ggacgttcgg tcgcgcggcg gtgttcgcct
     2401 tctatcccaa caaacagatg acgaccggcg agggtggaat gatcgtcacg agtgaccctg
     2461 agtggaacga tcgattccgt tcgctccgca atcaagggcg cgatattttt gatgcctggc
     2521 tgacgcattc ccggctcgga tacaactatc ggctcagtga actgggcgcc gcgctgggag
     2581 tggcgcaggt cagccgtctc gatgcgctgc tctgccagcg cgcgcgtgtt gcgggttggt
     2641 acaaccagcg actcagcggg caggacgccg ttcgtatttg gtcggttgcg aagacgacga
     2701 ccacgatgtc ctggttcatt tacgtcgtgc ggttagccga gggtgtggcg agagaccgcg
     2761 tcatggctga attggagaaa cgaggaattc cctctcgtcc gtacttctca ccgattcacc
     2821 ttcagccgtt gtatgtccag cgattcggat acaagccagg ccgttatccg gtcgcggagg
     2881 cgttgggtgc gtcctgttta gcgctgccgt tttcgggggt catgaccgaa acgcaggtcg
     2941 attacgtgtg tcagaacttg atagaggtgg tacatcactg cggggaccac cgtggagcgc
     3001 tcgcaagata gggcgttgcc atcccatcaa gtatttaggg ggcagcgcct gttcgtgtcc
     3061 ctgtgcgcag cgtgatatgg cgcggcattg gccctgcatc cgcgcgatat tcccggattg
     3121 acttcggttg ccgacgtccc tatagtcggc gcaacggttg gcgatgaggc gcgatggaac
     3181 aaggacgttc tttcggtcgt cgtttactgc aacgtgttct gaccccgttt ctcggccttc
     3241 ctagcggcct gcgccgcggg ttggtgtccg ccgtgttcct cctggagctt gcggtcgcca
     3301 cggggctggc gtggctggtg cgcttcgagg gcgacgtgcc ctccggcggg tgggacctcg
     3361 cgtggtccgc gtttccgctg atcgccgccg tgtacctgat cgcgtacgtg ctgttcggcg
     3421 cgcaccgcgg cctctggtcc ttcaccgggc tccacgattt cgtcaagatc ccgaaggtgg
     3481 cgctggccgg cgcaatcggg tcgtatgtcc tcacccgcga cgtgctcggc tggatcaatt
     3541 acccccgctc catttacatc ctgaccgcgg tgttcttgtt cgtgttgatg gcgctggatc
     3601 gcggcggggt gcgtctgctc cagcagcagg cccaccgctg gggcaagtcc ggcattccgg
     3661 tggtcattgt gggggcgggg caggcgggcg aaatgctcgt gcgcgacatg ctgcacgccg
     3721 aggcaggcgc gtatcgcccg gcggcgttca tcgacgacga cgcgagcaaa cacggatcca
     3781 ccatccacgg cgttccggtg gtcgggggac acgcggccct ggccgcggcg atcgcgcgct
     3841 accagccgca ggaagtgatc atcgcgattc cgtccgcgtc cccggcgcag ttgcggcgca
     3901 tcgtgggcgc ctgccagccg tgcgggttgc cgatcaaggt gttgccgagc gtgcacgaca
     3961 ttctgcgggg gcgggtggcg ctctcgtcgg tgcggccgct cgcgctggcg gacctgctga
     4021 cgcgcgagcc ggtgccgtcg gatacggcgg ggctggcgtc gctgattcgc ggcaagcgcg
     4081 tgttggtgac cggcgccgga gggtcgatcg ggtccgagtt gtgccgtcag atcgcccagc
     4141 acgcgccggc ccaattggtg ctggtggagc gctacgagaa caacctgcac gccgtggccg
     4201 ccgcgatcga cgatctctac ggcgcggagt tcgtcgacac ccgggcgttc ctggccgacg
     4261 tgctcgatga gttgcggatg cagcggatct tcgaggagca ccgaccgcag ctcgtgtttc
     4321 acgcggccgc gcacaaacac gtgccgatcg tggaagagaa cccgtgcgag ggcgtgctca
     4381 acaacgtgtt gggcacgcac cgggtggcga tgttgtgccg gcgctggtcg gtcgagcgca
     4441 tgatcctgat ttcgaccgac aaggcggtca atcccaccaa cgtcatgggc gcgaccaaac
     4501 gtttcgcgga atccatcgtc accgcgttga gcgggacggg cccgacccgc tacatcacgg
     4561 tgcgcttcgg caacgtgctc ggcagcaacg gcagcgtggt gccgcgcttt caacagcaga
     4621 tcgcgcgcgg cggtccggtg accgtgactc atccggagat cgagcggttc ttcatgctca
     4681 tcccggaagc ggtccacctg gtgctggaag ccgcgcggcg cgggcggggc ggggaagtgt
     4741 tcgtgctgga catgggcgac cccatcaaga tcgtggatct ggcccataac atgatccggc
     4801 tggcggggtt cgtcccgaac gacgacatcc cggtcgtatt ttccgggctg cggccggggg
     4861 agaagctctt tgaggagctg ttcgagaaag acgagcgcgt cgagccgacc gaacatccca
     4921 agctgcggat ggccgtgtcg gacgcggcgt ggtccgagac cgagctgcgc gtcgccgtga
     4981 ccgcggtgac caccgcggcg cgagttggag acggcgacgg gttacgcgag gccctgcgcc
     5041 agttcgtccg ctcgtaccaa ccggaagccc ttccggccac gactcatgcc aacggctccg
     5101 gcgtcaacgg ggtgcccgtg gacgtggagg tgtcggtcgc gaccgtgcgc tccgaggcgc
     5161 ggaatcgcgc gtcgtcttga acgtcgcaag cccttccgct ggggcgttgg tcctatatca
     5221 accggcccgc gatgggtctt ccgccgactc gtgtgagtcc tccggctcga ttgacgcgat
     5281 gaaaggttgc gtggtacagt cggccgttca attggtcttc acggtgagga gcgattcatg
     5341 agtatgacgc cgtcttccgt tacctatcgc gttcgtccgg gccccgaggc ctttttgccg
     5401 cctgcagcgg ccagcatggg gatcgtgctc ccggatccgg gccaggccca catcgaaggc
     5461 gtgatcgttg acgaggcgca ggcgttcgaa gaagccgcgc gccggttcct gggcgccaag
     5521 gtgcccacga tcttccccgg ccccttggtc ctctggaact ggaacgagac gtccaagaag
     5581 aaggcggccg cgatcaagaa attgtacgac gccatgaaag agtgcgcgcc ggccggcagc
     5641 aagccgatgt tgatccccat ggccgattac cggcccaagt accccaagat caaccccgaa
     5701 atcgaaatca accccaacca ccccaacctc acgatctggc acaaccgcat cgacgtgtgc
     5761 atgttcgtgg gggtgcactg ccaccaggcc aatttggcgc tcaagatcat ccgcggcgga
     5821 accaactgct tcaccatcgc gatgtgcgcg caagccgggc atgaagacgc caacctctcg
     5881 ttccgcgacg cgagtccgga gaagattttg aagttggcgg aggcggtgcg tcgggtggcg
     5941 gcggggccgt ccggcaacgg cggcaagccc taagagcctg ttcatagagg gccgtctgcg
     6001 gcgttgtcgc tgcgcttccg gtgctcacgt gcgactcagc acgctccgct ccggtgct
//
LOCUS       DBNGTT010000278         3152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2998412, whole genome shotgun sequence.
ACCESSION   DBNGTT010000278 DBNGTT010000000
VERSION     DBNGTT010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2998412"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC16BC_06185"
     CDS             complement(<1..251)
                     /locus_tag="AC16BC_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241245.1"
                     /translation="MRLTLARKMLLGNLTLVLLILAVGLSALASLKALYTLGTNLVQV
                     DLPNIELAAQLEESLLVRDQYEQRSAQLRDHTLQALADE"
     gene            complement(357..602)
                     /locus_tag="AC16BC_06190"
     CDS             complement(357..602)
                     /locus_tag="AC16BC_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR3241246.1"
                     /translation="MDHTAIRIDAELNLRGVHCPYNFVRTKLKLEEMKVGEILAVTVD
                     AGEPARNVPRSVADDGHRVLEMTPLDSALRIVIEKVG"
     gene            849..1463
                     /locus_tag="AC16BC_06195"
     CDS             849..1463
                     /locus_tag="AC16BC_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013671.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR3241247.1"
                     /translation="MQTLSPTITMTPTAREQIMAAMNTDGRTAHGLRLTVKYAGTRNQ
                     TYGLSLVEKSTPDVNDVVVDVEGIRVFLEKQNITFVDGASIDFLNGPNGSGFKIDSPY
                     KPTAVEIPTNPPTGPVADAVQKVIREQVNPAVASHGGYVSLVDIRDNVVFVRFGGGCH
                     GCGMADVTLKQGVATMIKKAVPDVKDVLDITDHANGANPYYQAH"
     gene            complement(1724..1993)
                     /locus_tag="AC16BC_06200"
     CDS             complement(1724..1993)
                     /locus_tag="AC16BC_06200"
                     /inference="COORDINATES: protein motif:HMM:NF025572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HIR3241248.1"
                     /translation="MAILRKTPWLLIFFVLVGGLFGGVVAEILRAVAPSGPISALFLK
                     NVSFGATDPITIDLYLITVTFGAAVKINLLSIIGVLMGVYIYRQA"
     gene            2134..3054
                     /gene="trxB"
                     /locus_tag="AC16BC_06205"
     CDS             2134..3054
                     /gene="trxB"
                     /locus_tag="AC16BC_06205"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR3241249.1"
                     /translation="MRSVIILGSGPAGLTAALYTARANLKPLVVEGVQAGGQLTITTE
                     VENYPGFPKGILGPQLVQDMREQAERFGAEFFRGDVTKVDLSKPPFRLWVEGESEQSC
                     EALIIATGASAKLIGLESERRLMGRGVSACATCDGFFFRDRRVIVVGGGDTAMEEATF
                     LTKFASQVTVVHRRDRLRASKVMQDRARKNPKIDFVWNSAVEEILGGEHVERVRLRDV
                     NTSAVTERAVDGVFVAIGHQPNTSLFAGQLATNEVGYILTSNGTKTSVPGVFAAGDVQ
                     DPVYRQAVTAAGTGCMAAIDAERYLEARHG"
BASE COUNT          633 a          959 c         1010 g          550 t
ORIGIN      
        1 tgttcgtcgg ccagcgcttg caatgtgtgg tcgcggagct gagcgctccg ttgctcatac
       61 tgatcgcgga ccaggaggga ctcttccagt tgggccgcca attcaatgtt cgggaggtcg
      121 acttgaacca ggttggtgcc cagcgtgtag agggccttca gacttgcgag cgcggagaga
      181 ccgacggcca ggatcaacag aaccagcgtc agattcccca gcagcatctt gcgagccagg
      241 gttaagcgca tgtgggcacg ggcaattcgg gataacggcg cgagttgagt gtagcggagg
      301 gactcggcgt gtcaaccgcc gagggaagag cgattggacg gaacggcggt cggcggttat
      361 ccgacctttt cgatcacgat acgaagcgcg gaatcaaggg gggtcatctc cagcacccga
      421 tgaccgtcgt cggctacgct ccgagggaca ttgcgggccg gttctccggc atccacggtc
      481 acggccaaaa tctccccgac cttcatttct tcgagcttga gtttggtccg gacgaaatta
      541 tacgggcaat gcaccccgcg aagattgagc tctgcgtcga ttcggatggc cgtgtgatcc
      601 attgtgcgat tatatcgagc gtttttcagc ctggtcaagg accacgccct gcgaaagacc
      661 ggcggcgcct ccgatcctcc tgacttaaca gacgggtcgc cgctttcatt cccaagggcc
      721 atggctcggg cttcagatct gggactcgtc agggcaacgt gtccgggtaa cgccgcaaca
      781 cacccctgac aggggcgatc gcactatgat atgatgcaga ccatacgaac acctgacggg
      841 aggcagcgat gcagactcta tctccgacca tcaccatgac ccccacggcc cgcgagcaga
      901 tcatggcggc catgaatacc gacggccgca cggcccatgg cctgcgcctg accgtcaaat
      961 acgcgggaac ccggaatcag acgtacgggt tgagcctggt ggagaaatcc acccccgacg
     1021 tgaacgacgt cgtggtcgac gtcgaaggca tccgggtgtt tctggagaag caaaacatca
     1081 cgttcgtgga cggtgcaagc atcgactttc tcaacggtcc caacgggagc ggcttcaaaa
     1141 tcgacagccc gtacaagccg accgcggtcg agatcccgac caatccgccc accggcccgg
     1201 tggccgacgc ggtgcagaag gtgatccgcg agcaagtcaa cccggccgtg gccagccacg
     1261 ggggctacgt gtccctggtg gacatccggg acaacgtggt gttcgtccgc ttcggcggcg
     1321 ggtgtcacgg ctgcggcatg gccgacgtca cgctcaagca gggcgtggcg accatgatca
     1381 agaaagccgt gcccgacgtc aaagacgtac tcgacatcac cgaccacgcc aacggcgcga
     1441 atccgtacta ccaagcccac taacgcggca tcgccgcgga cgtttcgacc acctgctcgg
     1501 aggggcgttg cgatgcaacg cccctccgtt tttttgtccg ggcgcagcgc atcgccatcg
     1561 tatcgaacta cacgcactgg gtcggtgtcc gggcgccgtt tgagccccgg gcgctgggcc
     1621 tccatggact aagaagccga cccgcgctcc ggcggggggg agccccccga tcgcatacgg
     1681 actcaccgct gacgagcgtc agcgatctta gacaacggct gccctacgcc tgccggtaga
     1741 tgtagacccc catcaacacg ccgatgatgc tcagcaggtt gatcttcacc gcggcgccga
     1801 acgtgaccgt gatcaagtac aggtcgatcg tgatgggatc ggtcgcgccg aaggaaacgt
     1861 tcttgaggaa gagcgcgctg atcgggccgc tgggagccac ggcgcgcagg atctcggcga
     1921 ccacgccgcc gaacaatccg cctacgagga cgaagaaaat cagcaaccac ggtgtcttgc
     1981 ggagtattgc catccaggat atccttccgc gcgcgcttgt gcgaaacccg acgacagtaa
     2041 agccgttcac gggtcattgt caagacacgt acgcacattg atttggcgcg aggcggagtg
     2101 ctacgatgcc ccctcgacgc taggagccat ttaatgcgat ccgtgatcat tttggggtcg
     2161 ggaccggccg ggttgaccgc cgcgttgtac accgcccgcg ccaacctcaa gcccctcgtg
     2221 gtcgaaggcg ttcaggccgg gggccagctc accatcacta ccgaagtgga gaattatccg
     2281 ggatttccca aggggattct cggcccgcag ttggtgcagg acatgcgcga gcaggccgag
     2341 cgattcggcg cggaattttt tcgcggtgac gtgaccaagg tcgatctctc caaaccgccg
     2401 tttcgtctct gggtggaggg cgagagcgag cagtcgtgcg aggccctgat tatcgccacc
     2461 ggcgcctcgg ccaaactgat cggcttggaa tcagaacggc ggctgatggg ccgaggcgtg
     2521 tccgcgtgcg ccacgtgcga cggctttttc ttccgggatc ggcgcgtgat cgtggtcggg
     2581 ggcggggaca ccgccatgga agaagccacg ttcctcacca agttcgcgtc gcaggtgacg
     2641 gtggtgcatc gccgcgatcg gctgcgcgcg tcaaaggtga tgcaggatcg cgcgcggaag
     2701 aaccccaaga tcgactttgt gtggaacagc gcggtggagg aaattctcgg cggcgagcac
     2761 gtggaacgcg tgcgcctgcg cgacgtgaac acgagcgccg tgaccgaacg cgccgtcgac
     2821 ggcgtgtttg tcgccatcgg ccaccaaccc aacacctcgc tctttgccgg acagttggca
     2881 acaaacgagg tgggatatat tcttacctcc aacggcacca agaccagcgt gcccggcgtg
     2941 ttcgcggccg gcgatgtgca ggacccggtg taccgccaag ccgtgaccgc ggcgggcacc
     3001 ggttgtatgg ccgcgatcga cgcggaacgg tatttggaag cgcgtcacgg gtaagttgag
     3061 gagactatgg cagacgtcaa gaagctaccg gcgctccaac ggttcttgcc ggagaaaatg
     3121 cagaaggtgg ggctgttcga aaccccgagg ct
//
LOCUS       DBNGTT010000279        10104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3509909, whole genome shotgun sequence.
ACCESSION   DBNGTT010000279 DBNGTT010000000
VERSION     DBNGTT010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3509909"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1632
                     /locus_tag="AC16BC_06210"
     CDS             190..1632
                     /locus_tag="AC16BC_06210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3241250.1"
                     /translation="MEFSWMQLIAIAPEVVLTVGACAVLLFDLAVPRDQKDKIGYFAI
                     GVLLVACYGSWKLADVETDAFSGMFVLDPYSAYFKVLIYLSGALTILLSMTYLEVERI
                     HLGEYHAFVLLATAGMMVMVSAGDLIMIYLGLELLSISLYTMAGFKRYEGRSVEASAK
                     YLILGSFSSAILLYGISILYGIAGTTNLKALGAFFAQGAADNPGLFMSMGFLVVGFGF
                     KVAAVPFHMWTPDVYEGAPTPVTAFMSVAPKAASFAVFLRVFAEALGGLKAHWQPILI
                     TLAIATMIIGNVVAIVQTNIKRMLAYSSIAHAGYALIGIIVGGSLGTMSLMLYLMIYA
                     VMNLGAFGVVILLRKGTLRGEDITDFTGLAKTNPVAAIIMLIFMFSLAGIPPTAGFVA
                     KFYLFMGAVEAGMVWLAVVGVLLSAVSAYYYLRVVMVMYMREPAGRFDLATSPATQFA
                     LVVTTLAVLFFGIYPTPLIDYTKAAILQTP"
     gene            complement(1860..2771)
                     /locus_tag="AC16BC_06215"
     CDS             complement(1860..2771)
                     /locus_tag="AC16BC_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241251.1"
                     /translation="MTARHRVILAAVLLLTAFTYRNALHNGFVWDDTFTIVNNRALDS
                     LGSAGSWFTDPDTWSSRNEASYRPVITAGFGGDVALSGRNPAWFHAVNIGIHLAVIVL
                     AYLLAVRLWNHAGSALVAAGIVALHPINAEAVNYVSARSSLLTACFVLASVAAWSRAE
                     RGSGSVPWLAAGLGLGLGALALGTKETAVVLPLLIMAWDRATSGPDRPFTATIRASIP
                     WWVLVAAYLALRTWLLAGKVTERPIGDGAWQPVLLAIKIFLVSLWSWFVPTGSAVDHG
                     WAWRIGAGALPQDPGDRSGPRGRKVSP"
     gene            complement(2786..3670)
                     /locus_tag="AC16BC_06220"
     CDS             complement(2786..3670)
                     /locus_tag="AC16BC_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3241252.1"
                     /translation="MLDDRTVTPGLTASRDPRPESPEWRDAVAARLDALAAAAHESAS
                     EVWTMRDRFVAEEKRPNDWVTDADRAADLIIRGVKDARFPEDGWLSEESLVHTAAGAF
                     TWVVDPIDGTREFVEGLPEYAVSIGLVWQGRAVAGAIAHPPSGVVMAGAITTGLRPAN
                     TSQLPGTRGTAAGLRILVSRTDLSRDRFAGWRADLPLTAVGSIAYKLALVAYGEADAV
                     VSVTKKNPWDVVGGFGLLRAAGLVPRYLDEREHPVPAVNERLPPFLVARTPDIEAELF
                     AESQLQAQRFAAAKGKRP"
     gene            complement(3730..4419)
                     /locus_tag="AC16BC_06225"
     CDS             complement(3730..4419)
                     /locus_tag="AC16BC_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241253.1"
                     /translation="MDDIGTRTLIERLERLERANRRMRGLVLAVLGAAACAAVVGASA
                     PSKPTDDVTAKRFVVVDANNKPRATLGLSPHGSTGLRLYDEQQRELASLEVLPGSVPS
                     LTFFGPDGRTAVWLISWPDVTTGLAFGDEGGNALAMVTERNRMRLTMSDANGMARTEL
                     VAGTGELPGLLLTDGDGIPRAELSLNEEEDPHLLFGDQYGNDRARLALEQGRPIMELL
                     DEDGKRVWGKP"
     gene            complement(4630..5622)
                     /locus_tag="AC16BC_06230"
     CDS             complement(4630..5622)
                     /locus_tag="AC16BC_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3241254.1"
                     /translation="MKLEVDARVQRPGGFTLEASLRCDAEAVGVVGPSGSGKTTFLEA
                     VAGIEPGARVVLDGADCSLLPIHRRDMGYVTQDALLFPHLSVRANLSYSPRASALGDV
                     PTALGIEPLLDRMPRHLSGGERRRVALARAILSRPRVLLLDEPFAGLDEPRRRDALSL
                     LNDLRTRYRIPLILVSHLADEVIGLTDWAIRLEDGRVVAHGPSASVLRASETRVDNYF
                     TGTVISPHQVQVGPWVLSVLLPESLTGDVRLACYAHDILLAAKIPDGISARNCLPASV
                     VSLQSAGSAVLVTLETPPLRVLVTDEAVASLNLKPGSDVVAIIKATSIAVLGAR"
     gene            complement(5619..6290)
                     /gene="modB"
                     /locus_tag="AC16BC_06235"
     CDS             complement(5619..6290)
                     /gene="modB"
                     /locus_tag="AC16BC_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009805539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR3241255.1"
                     /translation="MTPVALSLLVAMTATLISLPFAVAAAWVLERTRGPWRGVFQTVI
                     MLPLVLPPVVIGYLLLKLFAPRGWLGRALESFGMPVAFHWLGAVVAATVVGFPLLVMM
                     TGLAMNAIDPRLETVSRTLGRSAWHTFWRVTVPLSWPGMLSGAALAFARGLGEFGATI
                     VLAGRIPGETETIPLRIYAELDAPGGEARVTGLVVVSVVICFICVLGARALDARYRKR
                     LELDR"
     gene            complement(6287..6997)
                     /gene="modA"
                     /locus_tag="AC16BC_06240"
     CDS             complement(6287..6997)
                     /gene="modA"
                     /locus_tag="AC16BC_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3241256.1"
                     /translation="MTRLIACVLGLILLIVAVVAPSMADDRPRPLVLFAAASLKEAVT
                     EVADEWSRRHAREVKLSFDATSILARQINAGAPADVFVAAAPEWIDEVSPRARANWLS
                     NRLVCVVPQDVASFDLKTADSLALANDQVPAGKYARAALEHLGIALPKRVIYGASVRG
                     VLSKVMAGGAQAGIVYATDAAVEPKVRIAFTFPEDSHPPIVYAAGLLTVEGAALFEAL
                     REPWAMAIARRHGFLDPE"
     gene            complement(7104..7640)
                     /locus_tag="AC16BC_06245"
     CDS             complement(7104..7640)
                     /locus_tag="AC16BC_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3241257.1"
                     /translation="MFKVLLAAVFGARIAPLDESVVRFRVWPNDLDTNLHMNNGRYLT
                     LMDLGRLDLMFRTGLGRVIVKQRWRPMVGTAVIRFRRGLSPFERYVLKTRIVAWDDKW
                     FWLEQRFERNNDVVAVGAIKGLFRDSRGNVPTAEVLALLGVTGTPTTPDWILAWQRTD
                     ADAIRPKSSEPSSPVSLR"
     gene            complement(7669..8247)
                     /locus_tag="AC16BC_06250"
     CDS             complement(7669..8247)
                     /locus_tag="AC16BC_06250"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR3241258.1"
                     /translation="MTEKWNKRINRCQCWETDDPLFLVYRDEWGVPVRDDRLLFEQLV
                     LSGFQAGLSWRTILYKRENFRRAFHNFAIDRVAAYGARERRRLLADAGIIRNRQKIQS
                     AINNAKAVLTVRDEFGSLSDYLWDFVGERPIRRRHQGGRNPDRSPDSDALSKDLQTRG
                     FSFVGSTIVYAFMQSVGLVNDHRRSCPRARLK"
     gene            complement(9197..9481)
                     /locus_tag="AC16BC_06255"
     CDS             complement(9197..9481)
                     /locus_tag="AC16BC_06255"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR3241259.1"
                     /translation="MKQPLDLAKRFLALADRDIKTFLLLARANDIDDEAVGFHAQQAI
                     EKCLKAVLALHRIPFRRTHDLDELPGLDRHEAREIVNTVRQWAEGQVNQH"
     gene            complement(9465..9785)
                     /locus_tag="AC16BC_06260"
     CDS             complement(9465..9785)
                     /locus_tag="AC16BC_06260"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3241260.1"
                     /translation="MVPDQQIRQAVKILVDAARPSKVILFGSYGRGDAREGSDLDLLV
                     IQPTVTDKLAEMVRLRNALRPLRIPVDVVVASEQEIEDWGGLPGTLYYWALKEGTVLH
                     EAAA"
     gene            complement(9842..>10104)
                     /locus_tag="AC16BC_06265"
     CDS             complement(9842..>10104)
                     /locus_tag="AC16BC_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241261.1"
                     /translation="PGLFARIHIAMPKSDTYVVVPLLIATGPGAAARFSIGLVIATGM
                     TIGTAFTLFVVPAGYLYLGRDYAAQRAGEIPSTVPASGEVRA"
BASE COUNT         1835 a         3247 c         3184 g         1838 t
ORIGIN      
        1 tcccttgctg gcggccgtgt tctggatcgg gctgtacccg gcgccgttac tcgacgtgtt
       61 gcacgcgtcg gtgaatcacc tgctggcgca gacgcaggtg tccggcgggg aggtcgcgag
      121 cctgggatgg tccgacctgt ggggcatggt gcgcgcggcg ttgtcgtcgt agagatgtcg
      181 gggggagtga tggaattttc ctggatgcaa ttgatcgcga tcgcgcccga agtggtgttg
      241 acggtgggcg cgtgcgccgt gctgctgttc gacctggccg ttccgaggga tcagaaggac
      301 aagatcggct actttgcgat cggcgtgttg ctggtcgcgt gttacggatc ctggaagctc
      361 gcggacgtgg aaaccgacgc gttctccggc atgttcgtgc tcgatccgta ttccgcgtac
      421 ttcaaggtgt tgatctatct ctctggcgcg ctgaccattc tgctctccat gacgtacctg
      481 gaggtggagc ggattcatct gggcgaatac cacgcgttcg tgttgctcgc caccgcgggc
      541 atgatggtga tggtgtccgc cggcgatttg atcatgatct acctgggcct tgagctgctc
      601 tcgatctctc tgtacacgat ggcgggattc aagcggtacg agggccggtc ggtcgaggcc
      661 tccgccaaat acctcatcct ggggtcgttt tcgtcagcga tcctgctcta cggcatttcg
      721 atcctctacg gcatcgcggg cacgaccaac ctcaaggcgc tgggggcgtt cttcgcgcag
      781 ggcgcggcgg ataaccccgg gttgttcatg agcatgggat ttctggtcgt gggcttcggg
      841 ttcaaggtcg cggccgtgcc gttccacatg tggacgccgg acgtgtacga gggcgccccc
      901 accccggtga ccgcgttcat gtccgtggcg cccaaagccg cgagcttcgc ggtattcctc
      961 cgggtgttcg cggaggcgct cggcgggctg aaagcccact ggcaaccgat cctcattacc
     1021 ttggcgatcg cgaccatgat catcggcaac gtggtggcga tcgtgcagac caacatcaaa
     1081 cgcatgctcg cgtattcgtc catcgcgcac gcgggctacg ccttgatcgg gatcatcgtg
     1141 ggcggcagcc tcggcaccat gagcctgatg ttgtatctga tgatctacgc ggtgatgaac
     1201 ctgggcgcgt tcggcgtggt gatcctgctg cgcaagggca cgctgcgcgg cgaggacatc
     1261 acggacttca cggggctggc caagaccaac ccggtggcgg cgatcatcat gctgattttc
     1321 atgttctccc tggccggcat tccgccgacc gccggtttcg tggccaagtt ctatctgttc
     1381 atgggcgcgg tggaggccgg gatggtgtgg ctggccgtgg tcggcgtgtt gctctccgcg
     1441 gtgtccgcct actactacct gcgcgtggtc atggtcatgt acatgcgcga acccgccggc
     1501 cgcttcgacc tggccacctc acccgcgacc cagttcgccc tagtggtgac cacgctcgcg
     1561 gtcctgttct tcggcatcta ccccacgccg ctgatcgact acaccaaggc cgcgatcctg
     1621 caaacgccgt agcgggctgc tgaaaaagtc cgtctgcggc gttgctgcgc catccgtaag
     1681 gaacgtggat tatagcctcg ctccttcggc gcaacgcgga ttctcggtcg tcgtgactcc
     1741 tcgcgtacca cccagtacgc tccggggtca ctccttcctg cggccttgca gccggatctt
     1801 tttgagcagc ccacttgtag taggccgggc cataggcact acgttatgga ccaggcctgt
     1861 taaggcgaga cttttcgccc gcgtgggccg gatcgatcac caggatcctg cggtagcgcg
     1921 ccagcgccga tgcgccacgc ccacccgtga tccaccgccg agccggtcgg cacgaaccac
     1981 gaccagagcg agaccagaaa gatcttgatg gcgagcagga cgggttgcca cgcgccgtcg
     2041 ccgatcggac gctccgtgac cttgcccgcg agcagccagg tgcgcaaggc caggtaggcc
     2101 gcgaccagga cccaccacgg gatggaagcg cggatggtgg cggtgaatgg gcgatccggt
     2161 cctgacgtgg cgcgatccca cgccatgatc agcaggggca ggacgaccgc ggtttccttg
     2221 gtgccgagcg ccagcgcgcc gaggccgagg ccgaggccgg cggccaacca cggcacgctc
     2281 ccggacccgc gctcggcgcg cgaccaggcg gccacgctgg ccagcacgaa gcacgcggtc
     2341 aacagcgagg atcgggccga cacgtagttg acggcttcgg cgttgatcgg gtgcagcgcc
     2401 acgatgccgg ccgcgacgag cgcgcttccg gcatggttcc agaggcggac ggccagcaga
     2461 tacgcgagca cgatcaccgc gaggtggata ccgatattca cggcgtgaaa ccaggccgga
     2521 ttgcggccgg acaacgcgac gtcgcccccg aaaccggccg tgatcacggg tcggtagctc
     2581 gcctcattgc gcgagctcca cgtgtcggga tcggtaaacc acgaccccgc ggaaccgagc
     2641 gaatcgagcg cgcgattgtt cacgatcgtg aacgtgtcat cccacacgaa tccgttgtgg
     2701 agcgcgttgc ggtaggtgaa cgcggtcagc aggaggaccg cggcgaggat gacgcgatga
     2761 cgagccgtca cgggacgatc gagcgttacg gccgcttccc cttcgcggcc gcgaaccgtt
     2821 gggcctgaag ctgcgattcg gcgaacagtt cggcttcgat gtccggcgtt cgcgccacca
     2881 ggaacggggg gaggcgttcg ttgacggccg ggaccgggtg ctcgcgctcg tccagatagc
     2941 gcggaacgag tcccgcggcc cgcagcaggc cgaatcctcc gaccacgtcc cacgggttct
     3001 tcttggtgac gctcaccacc gcgtcggctt cgccgtaggc gaccaacgcc agcttgtacg
     3061 cgatgcttcc caccgcggtc agcggcaggt cggcccgcca cccggcgaac cgatcgcggg
     3121 acaggtcggt ccggctgacc agaattctga ggccggcggc ggttcctcgg gtgccgggaa
     3181 gctgcgacgt gttggcgggg cgcaacccgg tcgtgatcgc gccggccatg accaccccgc
     3241 tcggtggatg cgcgatcgcg ccggccacgg ctcgtccttg ccagaccagg ccgatggaca
     3301 ccgcgtattc gggaaggccc tcgacgaact cgcgggtccc gtcgatgggg tccaccaccc
     3361 aggtaaacgc gccggccgcg gtgtgcacca ggctctcttc cgacagccag ccgtcctcgg
     3421 gaaatctggc gtccttcacg ccccggatga tcaggtcggc ggctcggtcg gcgtcggtca
     3481 cccagtcatt cgggcgcttc tcttccgcga cgaaccgatc gcgcatggtc cacacttcgg
     3541 aggcggattc atgggccgcg gccgccaacg cgtcgaggcg cgccgccacg gcgtcccgcc
     3601 actcggggga ttcggggcgc gggtcgcgcg aagcggtgag tcctggcgtc acggtgcgat
     3661 catcgagcat ccaacgccat catcgcattg aaccgcttcg aagacaaccg gagccgttcg
     3721 ccagagtgat cacggcttgc cccatacccg cttcccatcc tcgtcgagca actccatgat
     3781 cggccggcct tgttcgagcg cgagtcgcgc gcgatcgttg ccgtactgat cgccgaacag
     3841 gaggtgggga tcttcctcct cgttcaacga cagttcggcg cgcggaatgc cgtcgccgtc
     3901 cgtgagcaac aggcccggca actcgccggt tcccgccacc aattcggtgc gggccatccc
     3961 gttggcgtcc gacatggtca ggcgcatccg gttgcgttcc gtgaccatgg ccagcgcgtt
     4021 gccgccctcg tcgccgaacg ccaggccggt ggtcacgtcg ggccaggaaa tcagccacac
     4081 cgcggttctg ccgtcgggtc cgaagaacgt gagcgagggc acggatccgg gcagcacttc
     4141 caggctcgcc agctcccgct gctgctcgtc atagagccga agccccgtgg agccgtgcgg
     4201 ggagagcccg agcgtcgcgc gcggtttgtt gttcgcatcc accaccacga agcgcttggc
     4261 cgtgacgtcg tccgtcggtt tggagggggc cgacgccccg accaccgcgg cgcaggccgc
     4321 cgcgcccaac accgcgagga caaggccccg catccggcgg ttggcccgct cgaggcgttc
     4381 aagacgctca atcaacgtgc gtgtcccgat atcatccatg atgacctccg tggcttgggt
     4441 ccagtggccg gccgcgaatc aacgcgcaga cgcggcgacg cgattccggg cgtcacttta
     4501 agggattccg ctccaagggg caagaccggt cgggcaatcg cgatcgcggc gcgttatcat
     4561 ggaccggcca acaggccgat caccgctcta tcccgcgact caacgcgccg tcatgccgac
     4621 gggggcgggt catcgcgcgc ccaacaccgc gatcgaggtg gccttgatga tggcaaccac
     4681 gtctgatccc ggtttcaggt tgagcgacgc aaccgcctcg tccgtgacca gcacgcgcaa
     4741 cggaggcgtc tccagcgtga cgagcaccgc cgagcccgcg gactggagcg acacgacgct
     4801 cgcgggcaga caattccggg ccgagatccc gtccgggatt ttggcggcca gcaagatgtc
     4861 gtgcgcgtag caggccaggc gaacgtctcc ggtcagggac tccggcagga gcacggagag
     4921 gacccacggg ccgacctgga cctgatgcgg gctgatcacg gtgccggtga agtagttgtc
     4981 cacgcgggtt tcgctcgctc ggaggaccga cgcgctgggg ccgtgcgcca ccacgcgtcc
     5041 gtcctcgagg cgaatcgccc aatccgtgag cccgatcacc tcgtcggcga ggtggctcac
     5101 gaggatgagg ggaattcggt atctcgtgcg gaggtcgttc agcagcgaaa gcgcgtcgcg
     5161 ccgccggggc tcgtccagcc cggcgaacgg ttcgtccaac agcagcacgc gggggcggct
     5221 caggatcgcg cgggccagcg ccacccgacg ccgttccccg ccgctcaaat gccgcggcat
     5281 ccgatccagc aacggctcga tgcccaacgc ggtcgggaca tcgcccaacg cggaggcgcg
     5341 ggggctgtac gacagattgg cccggacgct caggtgagga aacagcagcg catcctgggt
     5401 cacgtatccc atgtcgcgcc ggtgaatcgg cagcaaggaa cagtccgcgc cgtccagcac
     5461 cacgcgcgcc ccgggctcaa tgccggccac ggcttccagg aaggtcgtct tgccgctgcc
     5521 gctgggcccc accacgccca cggcctcggc gtcgcagcga agggacgcct ccagcgtgaa
     5581 tcctccgggg cgttgcaccc gcgcgtcgac ctcgagtttc atcggtccag ttccaatcgc
     5641 ttgcgatacc gggcgtccaa cgcgcgcgcg cccaggacgc agataaaaca gatcacgacc
     5701 gagaccacga ccagacccgt gacgcgggcc tcgccgcccg gagcgtccag ttccgcgtag
     5761 atcctgagcg gaatggtttc ggtctcaccc ggaatccgtc cggccaggac aatggtggcc
     5821 ccgaactccc ccagcccgcg cgcgaacgcc agcgcggcgc cggacaacat gccgggccac
     5881 gagaggggaa ccgtcacgcg ccagaaggtg tgccaggccg agcggcccag ggtccgcgac
     5941 acggtttcca gtcggggatc gatcgcgttc atggccaagc cggtcatcat gaccaggagc
     6001 gggaatccga ccaccgtcgc ggccacgacc gctcccagcc aatggaatgc caccggcatg
     6061 ccgaagctct ccagcgcgcg ccccaaccaa ccccgcgggg cgaagagctt caacagcaga
     6121 tacccgatca ccacgggcgg gagaacgagc gggagcatga tcacggtctg aaacacgccg
     6181 cgccacggcc cgcgcgtgcg ttccaggacc caggccgcgg ccacggcaaa gggcaagctg
     6241 atgagcgtgg ccgtcatggc cacgagcagt gagagcgcga ccggcgtcat tccggatcga
     6301 ggaacccgtg tcggcgggcg attgccatgg cccacggttc ccgcagcgcc tcgaacaacg
     6361 ccgcgccctc gaccgtaagc agcccggccg cgtacacgat cggtgggtga ctgtcctccg
     6421 gaaacgtgaa cgcgatccga accttgggct cgacggccgc gtcagtggcg tatacgatgc
     6481 cggcctgggc cccgccggcc atgaccttgg agagcacgcc gcgcacgctg gcgccgtaga
     6541 tcacccgttt cgggagcgcg atccccagat gctccagcgc cgcgcgcgcg tattttccgg
     6601 ccggaacctg gtcattggcc agagccaggc tgtccgcggt tttcagatcg aacgacgcga
     6661 cgtcttgggg caccacgcac accagacgat tggacagcca gttcgcccgc gccctgggcg
     6721 agacctcgtc gatccactca ggcgccgcgg ccacgaacac gtccgccggg gcgccggcgt
     6781 tgatctggcg agccaggatc gaggtggcat cgaacgagag tttgacctcg cgcgcatggc
     6841 gacgtgacca ttcgtccgca acctcggtaa cggcctcctt caaacttgcc gcggcgaaca
     6901 gcacgagcgg tcgaggccga tcgtcggcca tggacggggc aacgaccgcg acgatcaata
     6961 aaatcaaacc gagtacgcag gcaatcaggc gcgtcatggg cgatgtcgtg agcctacgcg
     7021 cctgatccag tccgagtcaa ctggcggaac atcgccaacg tttcacggcg agggcgggtc
     7081 ccccgtaacg gcgagcgact gcgctacctg agggataccg gtgaggatgg ctcggaagat
     7141 ttcggtcgga tcgcgtccgc gtccgtccgt tgccacgcga gaatccaatc cggggtcgtg
     7201 ggcgtccccg tcaccccgag caatgccaag acctcggcgg tcgggacatt cccgcgcgag
     7261 tcacggaaca gccctttgat cgcgccgaca gccaccacgt cgttgtttcg ttcaaagcgt
     7321 tgctcgagcc agaaccattt atcgtcccac gccacgatcc tggtcttgag aacataccgc
     7381 tcaaatggcg ataacccgcg gcgaaaccgg atcaccgccg tgcccaccat gggccgccac
     7441 cgctgtttca cgatcacgcg acccagcccg gtgcgaaaca tcagatccag acgccccaga
     7501 tccatgagcg tcaggtaccg gccgttgttc atgtgcaagt tggtgtcgag atcattgggc
     7561 cagacgcgaa accgcacgac cgactcgtcg agcggcgcaa tccgcgcccc gaacacggcg
     7621 gccaggagga ccttgaacat tcggacaaag aggttcacgc ccgatagttt acttgagccg
     7681 ggcccgtggg catgatctgc gatgatcgtt gaccaatccc acgctttgca tgaacgcgta
     7741 gacgatggtg gagccgacga aactgaaccc gcgcgtttgc agatctttgg acaatgcgtc
     7801 ggagtcggga gaacgatccg gattccgacc gccctggtgt cgccgtcgaa tcggccgctc
     7861 gccgacaaaa tcccacaggt agtcagatag ggagccgaac tcgtcccgca ctgttagcac
     7921 ggctttggcg ttgttgattg ctgactggat tttctgccgg ttgcggataa ttcccgcatc
     7981 agccaacagc cgacggcgct cgcgcgcgcc gtatgccgcg actcgatcga tagcgaagtt
     8041 atggaacgcg cgccggaagt tttcgcgctt ataaagaatt gtccgccatg acaacccggc
     8101 ctgaaacccg ctcaacacca actgctcgaa caacagccga tcgtcccgta ccggcacgcc
     8161 ccactcgtcg cggtagacga ggaataaagg atcatcagtc tcccaacatt ggcatcggtt
     8221 gatccgctta ttccattttt ctgtcatgga gagggtctat agtatgacaa tctcgaagat
     8281 ggactaaacc atcacgtctg cgatctcttg aagttgagga acgcatcgga ggtaaccgcc
     8341 tgactacaga taagcccgtt cttgtggaga tgatgaattg cctctcgcag tgcggagtca
     8401 ttggtaatga aaaattgcac cgtatctata tatgcacaat ggcaagcgtc gttggagtcg
     8461 atcgttttcc catgtttaac cccaacctgt tgcagcccat gcctatacat gaaatagatc
     8521 tgtgtggtta gccatccacg agtgaaaggg caaaactgta tgaagtcgtt gattcgtgga
     8581 gtgatctctc tctcaaaatc ttttagaact gatggcagta gtgcccacct aataaactga
     8641 ggaagttcgt cattcacgaa tgattttatg tctggcggga taaccccatc cgcttgtctc
     8701 aattgatcaa ggaccggcag tcgcccattc tggccagtcc gtgcgtcttt gtgagcctcc
     8761 ttccatcgtg tctttccctt tctaaggaag gattgcattc cgcgcgaatg atgaggtttg
     8821 tcaagccatc ctcgcaacat atctgatcgt tctgatttct gccaatccca ctgcttttct
     8881 ccatctagaa accgttttgc cgagttttta acctgctgct gccatcttgc taggacgtgc
     8941 tctcccgcca gcgcctccaa tactgctttg gcagtgcgac tccgttcgac ctcattggga
     9001 atcgcgaaga tctcagccag agtgtgttcg gttacaaccg ctttataacc gccaagcatt
     9061 ggccctatac ccgtgcagtg tctgtatacg ttcgtgtcga ggaaaacctt gtttgtcatt
     9121 tggtgaatct attaaggata aaagatggcg gatctatttg ctgtaatggt tcgattcaca
     9181 tgccatttgt tggcgcttag tgttgattca cctgcccctc ggcccattgg cgaacggtgt
     9241 tcacgatctc cctcgcctcg tgacggtcga gccctggaag ctcgtcgagg tcatgagttc
     9301 ttcgaaaggg gattcggtgg agggcgagga cggctttgag gcacttttcg atggcctgct
     9361 gggcgtggaa cccaaccgcc tcatcgtcga tgtcgtttgc ccttgcgagg aggaggaacg
     9421 tcttgatgtc gcggtccgcc agcgcgagga agcgcttggc cagatcaagc ggctgcttca
     9481 tgcaggacgg ttccttcctt gagcgcccag tagtacagcg ttccgggaag acctccccaa
     9541 tcttcaatct cctgttcgga agccaccacc acgtccacgg ggattcttaa cggacgaagc
     9601 gcattccgca gtcggaccat ttcggccagc ttatccgtca ccgtcggttg gatcaccaat
     9661 aaatcgagat cggacccctc gcgtgcatcg ccccgcccat atgaaccgaa caggatgact
     9721 ttggatggcc gtgcggcgtc gacgaggatc ttgactgcct gcctgatctg ttggtcagga
     9781 accatatatc tggcagtata tgacaatctt cacaggccag acaagcgagc ggacaaggtc
     9841 cctatgcacg tacttcgccc gaggcgggca ctgttgatgg aatttctccg gcccgttgcg
     9901 ccgcgtagtc tcggcccaag tacaagtacc cggccggcac cacgaacaga gtgaacgcgg
     9961 tgccgatggt catgccggtg gcgatgacca agccgatgga gaaacgtgcg gcggcgccgg
    10021 ggccggtggc gatcaagagc gggaccacca cgtaggtgtc cgacttgggc attgcgatat
    10081 ggatgcgcgc gaagagcccg ggtt
//
LOCUS       DBNGTT010000280         6093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1337775, whole genome shotgun sequence.
ACCESSION   DBNGTT010000280 DBNGTT010000000
VERSION     DBNGTT010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1337775"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="AC16BC_06270"
     CDS             complement(<1..286)
                     /locus_tag="AC16BC_06270"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3241262.1"
                     /translation="MNSPVELYKTYDPLEADRIADLLADHGIWCAVRDLTMSPYPMTI
                     GRFGERRISVAPQDRERAREVLEDAIRDGYVSRDGAWIEDGHESPPPRRRA"
     gene            409..1083
                     /gene="ccmA"
                     /locus_tag="AC16BC_06275"
     CDS             409..1083
                     /gene="ccmA"
                     /locus_tag="AC16BC_06275"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR3241263.1"
                     /translation="MSAVRVIELAKAHGHYRVFERVSFELLPGECLAVFGPNGAGKTT
                     LIRVLATLSRPSAGRFEIDGKDGVRDKDAVRTSLLLLAHGSHLYDELDGAENLRFSLA
                     LRGLTLRDRDIKSALDRVGIGAFSDLKTRYYSAGMKKRLTIAKSMLVRPKVLLLDEPY
                     TSLDEAGIALVNEHLRALTRDGAAVLMSTHDRARSADVAHRAGVLDKGHWREVAVERL
                     KTDALF"
     gene            1070..1747
                     /locus_tag="AC16BC_06280"
     CDS             1070..1747
                     /locus_tag="AC16BC_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR3241264.1"
                     /translation="MPFFNVIRWVAWKDFISEVRSRETLSSMFFFALIVILVFSFSFS
                     MDQQAAQELIAGIMWVAFTFTGIIGLGKSFSAELQNDSLENLQISPTSKGAIFLGKLA
                     ANLLFMLAVEIILFPLFVIFFNLNVVEALPVLLLVFALATLGLSTVGTLFSAMTVQVR
                     AREVMLPILLLPLAVPVMIAAVESTRGALSGSPLSLYSNWLQLLGVFDVVFTVLSFWA
                     FEWILDT"
     gene            1758..2480
                     /gene="ccsA"
                     /locus_tag="AC16BC_06285"
     CDS             1758..2480
                     /gene="ccsA"
                     /locus_tag="AC16BC_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR3241265.1"
                     /translation="MNALIKWMQRYEGWFGAVGTACIIVGLYLALAGSPPDYYQGEVV
                     RIMYIHVPLVQTAMLAYSVLFGGSIWYLWKRDPIVDNMCHAAAGISAFLTAGGLVTGS
                     IWAKPTWNTWWTWDARLVSFAVMLMILVGYLMLRTFVDDPEREARYAAVLAIVGFVDL
                     PIVHFSVEWWRTLHQPLSISMRGIAMTWEMGRPLMVMSAGFGILFVYILMVRTQMYYL
                     THLLLAKKGRLLSHAHLSDLPT"
     gene            2477..2989
                     /locus_tag="AC16BC_06290"
     CDS             2477..2989
                     /locus_tag="AC16BC_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015085.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE
                     domain-containing protein"
                     /protein_id="HIR3241266.1"
                     /translation="MTRLYIQWAVIALAAAGIVWAASRYYDRHLVTMTPEAVQGSESG
                     TSAVRVQGLVEGGTLAGDLVSGKATFRLAGEHGTLDVAYDGPPPENLRELKTVVVVGR
                     WDAGRRVFLAQDLALVTNYGFVVGSYLVALLPLAVFIFLMERRVRLLYSEIKQAKLYE
                     PEPTTHVDAR"
     gene            2973..3428
                     /locus_tag="AC16BC_06295"
     CDS             2973..3428
                     /locus_tag="AC16BC_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015085.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR3241267.1"
                     /translation="MWTRGKTIGIVVSLLLVGGALAYLALGNFGENLVYFFTPSEVSA
                     FTPEYFNKKVRVGGMVVKGSVKSTPQPVGMTFELTDGAATIPISFEGIPPDLFKEGQG
                     AVVEGVWTPTKEFHSTMIMAKHSEDYMPIELRRAGTELPKKDFLKTLRQ"
     gene            3480..5429
                     /locus_tag="AC16BC_06300"
     CDS             3480..5429
                     /locus_tag="AC16BC_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR3241268.1"
                     /translation="MIIELGHFAVVTALVLSVLGVIAPIIGLRTKNPDWLRSGRQTVT
                     LIFVLLAVGIATLEYAFLTRDFSVAYVAATSNSKLPIFYTVAALWGGHEGSLLLWVFI
                     LSAFSTVAVWLHWRTQPAIMPYLMAVESAVIFGFLMLIVFLSNPFDRIFPAPVDGKDL
                     NPLLQDPAMTMHPPMLYMGYVGFSIPFAFAMAALFSGRLGEEWIKVTQRWTIFAWLCL
                     TTGIMMGGYWAYYELGWGGYWGWDPVENASFMPWLVGTAFLHSVMVQEKRKMFKVWNL
                     FLVILTFSLSLIGTFLVRSGVLTSVHSFAADPERGVFILLFLAVVMGTAFGTLMVRAP
                     KLKSQIQLDSVVSRESVFLFNNLFFLVAAATVFLGTLYPLMIETWKGSKVTVGPPYYN
                     AVFMPIALGLLFLMGIGPYIAWRKASIDNLKKNFLMPLIIAVAATLLAFGLGFRSPFS
                     LAGAFVVGFVATTIAVDVGKVSAFFSRRDHVNYLRGFFLAFRGNQRRYAGTLTHFGVL
                     ITVVGIIASTVYQTEKVVSLSVGDDFALGAYRVKLVDIHDVQGPNWTAREGRFELYRG
                     DRLLTELRPQKRVYAATQSPTTESAIYAINMGHVFLTMPEVTPEGTATARGVINPLIL
                     WVWGGGAIMGIGVLLNMFRPRKRDE"
     gene            5426..5986
                     /locus_tag="AC16BC_06305"
     CDS             5426..5986
                     /locus_tag="AC16BC_06305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3241269.1"
                     /translation="MTRFWQIVLACGLLGLFVLFYQGLWGNPRYIPTVLKNTPARAFS
                     APVLGGTEIISLDQLKGKVVLLNFWASWCQECRLEHSNLLALNEQFRHDPNFVMVGVN
                     YQDKEDDALEYIKTFGTNFSHVRDVKSAISFDYGVYGVPETFVIDQQGVIRCKTIGPI
                     MGSVYANYTQQILPALLKGSTESLAC"
BASE COUNT         1028 a         1868 c         1875 g         1322 t
ORIGIN      
        1 tcgctcgacg ccggggcgga ggcgactcgt gtccgtcttc gatccatgcc ccgtcgcgcg
       61 agacgtagcc gtcccgaatc gcgtcttcca acacctcccg cgcccgttcc cggtcttggg
      121 gcgccacgct gatccgccgc tctccaaagc gtccgatcgt catcggatac ggggacatgg
      181 tcaagtcccg aacggcgcac cagattccgt ggtccgccaa taaatccgcg atccggtcgg
      241 cctccagcgg gtcgtacgtc ttatacagtt ccacgggtga attcatgtcg gagttatgat
      301 aacgaaaccc cggacggacc gtcaatgtga gcggccgtag acattgatcg ggagcgtccc
      361 gtatactggg ccgattttat gccgcctacc cgttcaatga tcgcccccat gagtgccgtc
      421 cgcgttattg agctggccaa ggcgcatggg cactaccgag tgttcgaacg cgtgtcgttc
      481 gaactcctgc ccggcgaatg cctcgcagtg ttcggcccca acggcgccgg gaaaaccacc
      541 ttgattcgcg tgctggccac gttgtcgcga ccctccgccg gtcggttcga aatcgatggg
      601 aaggacgggg ttcgcgacaa ggacgcggtg cgcacgtcgc ttctgttgct ggcgcacgga
      661 tcccacttgt acgatgagct cgacggggct gaaaacctgc gtttctcgct ggcccttcgt
      721 ggactgactc ttcgagaccg cgacataaaa tcggcgctcg accgcgtggg catcggcgcg
      781 ttttcggact tgaaaacccg ttattactcc gcgggcatga aaaaacggct gacgatcgcc
      841 aagtccatgc tggtccgccc caaggttctg ctgttggacg aaccctacac gtcgctcgac
      901 gaggcgggga tcgcgttggt caacgagcac cttcgcgcct tgacccgcga cggcgccgcg
      961 gttctgatga gcactcatga ccgcgcgcgc tccgcggatg tcgcccaccg cgccggcgtc
     1021 ctggacaaag gccactggcg ggaggtcgcg gtcgagcgac taaaaaccga tgcccttttt
     1081 taacgtcatc agatgggtgg cctggaagga cttcatcagc gaggtccgca gccgggaaac
     1141 cctgtcctcg atgttctttt tcgcgttgat cgtgatcctg gtattcagct tcagcttttc
     1201 catggaccag caggcggcgc aggagttgat cgcgggcatc atgtgggtgg cgttcacgtt
     1261 taccgggatc atcggcctgg gcaaatcctt ttccgccgag ctccaaaacg acagcctgga
     1321 gaacctgcag atcagcccga cctccaaggg cgccattttc ctgggcaagc tggccgccaa
     1381 cctgctgttt atgctcgcgg tggaaatcat tttgttcccg ttgttcgtga ttttcttcaa
     1441 cctcaacgtg gttgaagccc ttcccgttct tttgttagta ttcgcgttgg ccacactcgg
     1501 gttgtccacg gtcggcacgt tgttttcggc catgacggtc caggtgcggg cccgcgaagt
     1561 gatgctgccg attctcctat tgcccctggc ggtcccggtc atgatcgcgg ccgtcgaatc
     1621 cacccggggc gcgctcagcg ggtccccgct ttccctttac agcaactggc tgcagttgtt
     1681 gggggtgttc gacgtggtgt tcaccgtgtt gtcattctgg gcgttcgaat ggatcctgga
     1741 cacataggct cgcggtcatg aatgcattga tcaagtggat gcaacgctac gaggggtggt
     1801 tcggcgccgt gggcaccgcc tgtatcatcg tgggcctgta tctggcgctg gcggggtctc
     1861 cgccggacta ctaccaaggc gaagtggtcc ggatcatgta catccacgtc ccgctcgtgc
     1921 aaaccgcgat gctggcctat tcggttctgt tcggcggcag tatctggtac ctctggaaac
     1981 gcgatccgat cgtcgacaac atgtgccacg ccgcggccgg catcagcgca ttcttgaccg
     2041 cgggaggact ggtgacgggc tcgatctggg ccaaacccac ctggaacacg tggtggacgt
     2101 gggacgcgcg tttggtgtcc ttcgccgtga tgttgatgat tctggtcggc tacctgatgc
     2161 tccgcacgtt cgtggacgac ccggaacgcg aggcgcggta cgccgcggtg ctcgcgatcg
     2221 tcgggttcgt ggatctgccg atcgtacact tttccgtgga gtggtggcgg accctgcacc
     2281 aaccgctgtc gatttccatg cggggcatcg ccatgacgtg ggagatgggc aggcccctca
     2341 tggtaatgag cgccgggttt gggatcttgt tcgtgtacat cctgatggtt cgaacccaga
     2401 tgtattacct gacccatctg ctgctcgcca aaaagggacg cctcctcagt cacgcccacc
     2461 tctcggatct gccgacgtga cccggctcta tattcagtgg gccgtgatcg ccttggccgc
     2521 tgcgggcatt gtctgggcgg cgtcgcgtta ctacgacagg cacttggtga ccatgactcc
     2581 ggaagcggtc caagggagcg aatccggaac gtccgccgtc cgcgtgcagg gtctggtcga
     2641 aggcggaacc ctggccggcg acctggtgtc cggcaaggcg acctttcggc tcgcgggcga
     2701 gcatgggacg ttggacgtgg cgtacgacgg accgccgccc gagaacctgc gcgaactcaa
     2761 aaccgtggtc gtggtcggcc ggtgggacgc gggacgtcga gtgttcctcg cgcaggatct
     2821 ggcgctggtc acgaactacg ggtttgtggt cggttcctat ctcgtcgccc tcctgccgct
     2881 cgcggtgttt atttttctga tggaacgtcg cgtgcgcctg ctctacagtg agatcaagca
     2941 agccaaattg tacgaaccgg agccaaccac ccatgtggac gcgcggtaaa acgatcggca
     3001 tcgttgtcag ccttctgctc gtggggggag cgctcgccta tctcgccctg ggcaacttcg
     3061 gcgaaaacct cgtgtacttt ttcacgccgt cggaagtctc ggccttcacg ccggagtatt
     3121 tcaacaagaa ggttcgcgtg ggcggcatgg tggtgaaggg cagcgtgaaa tcaactcccc
     3181 aaccggtggg catgaccttc gagctcaccg acggcgcggc cacgattccg atttcattcg
     3241 aagggatccc gccagacctg ttcaaagaag ggcagggcgc cgtggtcgag ggggtgtgga
     3301 cgcccacgaa ggaatttcat tcgaccatga tcatggccaa acactccgaa gactatatgc
     3361 cgattgagct caggcgcgcg ggaaccgagc tccccaagaa agatttcctc aagaccttgc
     3421 gccaataggg cgtcccctcg gctccttcac atgctttcac tggacacgcg gcgcgcatca
     3481 tgatcattga actgggacat ttcgcggtgg tcaccgcgct ggtgctctcc gtgctcgggg
     3541 tcatcgcccc gatcatcggc ctgcgcacca agaaccccga ctggttgcga tcgggacggc
     3601 agacggtcac gctgattttc gtcctgctcg cggtcggcat cgcgacgttg gaatacgcgt
     3661 ttctgacgcg ggacttctcg gtcgcgtacg tcgcggccac gtccaactcc aagctgccga
     3721 tcttctatac cgtcgcggcg ctctggggcg gccacgaagg ctcgttgctc ctgtgggtgt
     3781 tcatcctctc cgcgttcagt acggtggcgg tgtggctgca ttggcgcacg cagccggcca
     3841 tcatgccgta cctcatggcc gtggaatccg cggtcatctt cggcttcttg atgctcatcg
     3901 tgttcctgtc gaatccgttc gatcgcatct tccccgcccc cgtggacggc aaggatctca
     3961 atccgttgtt gcaggacccg gccatgacca tgcacccgcc catgctctac atggggtacg
     4021 tgggcttctc gatcccgttt gcgttcgcga tggccgcgct cttttccggc cgtctgggcg
     4081 aggagtggat caaagtcacg cagcgctgga ccatcttcgc gtggctctgc ctgaccaccg
     4141 gcatcatgat gggcgggtac tgggcctact acgagttggg ctggggggga tactggggat
     4201 gggacccggt ggagaacgcc tcgttcatgc cctggttggt gggaaccgcg ttcctgcact
     4261 ccgtgatggt gcaggagaaa cgcaagatgt tcaaagtgtg gaacctcttc ttggtcatcc
     4321 tgacgttttc attgtctctg atcggcacgt ttctggtgcg ctccggggtg ctcacctcgg
     4381 tccactcgtt cgccgcggac ccggagcgcg gggtgttcat cctgttgttt ctggccgtgg
     4441 tgatgggcac ggcgttcggc acgctgatgg tgcgcgcgcc gaaactcaag agccagatcc
     4501 agctcgactc ggtcgtgtcc cgcgaatccg tcttcctgtt caacaacctg ttctttctgg
     4561 tggccgcggc cacggtcttc ctgggcaccc tctacccgct gatgatcgag acgtggaagg
     4621 gctccaaggt cacggtcggg ccgccctact acaacgcggt gttcatgccg atcgcgctcg
     4681 gcctgctctt tctcatggga atcgggccct acatcgcctg gcgcaaggcc tcgatcgaca
     4741 acctcaagaa aaatttcctc atgccgctga tcatcgcggt ggcggcgacg ctgctcgcgt
     4801 tcggcctggg atttcggagc ccgttttccc ttgcgggcgc gttcgtggtc ggattcgtcg
     4861 ccaccaccat cgcggtggac gtgggcaagg tctccgcgtt tttctcgcgg cgtgatcacg
     4921 tcaactatct gcgcgggttc ttcctggcgt ttcggggcaa tcaacgccgc tacgccggca
     4981 ccctcaccca cttcggcgtg ttgatcacgg tggtcggaat catcgcttca accgtctatc
     5041 agaccgagaa agtggtctcc ctgtcggtcg gcgacgactt tgccctgggc gcctatcggg
     5101 tcaaactcgt ggacattcat gacgtccagg gtccgaattg gacggcgcgc gaaggacgat
     5161 tcgagctgta tcgcggcgac cgcctgctga ccgaactccg gccgcagaaa cgcgtgtacg
     5221 cggccacgca gagtccgacc accgagtcgg cgatctacgc catcaacatg ggccacgtct
     5281 ttctcaccat gcccgaggtg acgccggaag gcacggccac cgcccgcggg gtcatcaacc
     5341 ccctcattct ctgggtgtgg ggcggcgggg cgatcatggg tatcggcgtc ctgctgaaca
     5401 tgttccgtcc tcggaaacgc gacgaatgac ccggttctgg caaatcgtgc tcgcgtgcgg
     5461 attgctgggc ctgttcgtcc tcttctacca ggggctgtgg ggcaatccgc gatatattcc
     5521 cactgtcctg aagaacaccc cggcacgggc cttctccgcc ccggtgctcg ggggcaccga
     5581 aatcatttcg ctcgatcagc tcaagggcaa agtcgtgctg ctcaattttt gggcctcgtg
     5641 gtgtcaggag tgccggttgg agcacagcaa cctgctcgcg ctcaatgaac agttccgcca
     5701 cgaccccaac ttcgttatgg tgggtgttaa ttaccaggac aaggaggatg acgcgctcga
     5761 gtacatcaag acgttcggca cgaacttctc ccatgtgcgg gacgtcaaga gcgccatatc
     5821 attcgactat ggggtatatg gcgtcccgga aacgttcgtc attgaccagc agggcgtgat
     5881 ccggtgcaaa accattggtc cgatcatggg gtcggtctac gcgaactaca cgcagcagat
     5941 tctccccgcg ctcctgaagg ggtccacgga gtcattggcg tgttgagggc ccgacgactc
     6001 gtctgggcat tcgcggccgc gttgataatg gcgtccacgg cggccgcgac caccgtggaa
     6061 gaatcggacc atgagaagcg aactcgggaa gtg
//
LOCUS       DBNGTT010000281         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2359873, whole genome shotgun sequence.
ACCESSION   DBNGTT010000281 DBNGTT010000000
VERSION     DBNGTT010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2359873"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1379
                     /locus_tag="AC16BC_06310"
     CDS             192..1379
                     /locus_tag="AC16BC_06310"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3241270.1"
                     /translation="MSHAASCTDSRGGAGFIDRRVRGAGASEVLTYTVRLATAAEVRD
                     ARDRWNALVLKMPLPSFFCTWEWISTWQEVFGSQYEPVILFVYRGDELAGILPLCRRE
                     AVLTRGLMSGRILSYCGARELYPDHLDLIAPEEDAVACLNAIWAFLVEEYSAWDVLHL
                     SYLWGGSGLVSAITRPDFPLSLQTTQASFARYIHVSGTFDEFFDRFDKKFRYNVRSRR
                     KKLMEEHGMRYITCDAGRQPEALDLVFALNDRRFASKGVGSSLMAPSIIDFHRRLLSA
                     LAGTDRIQLRLLGNDEQIAAASYNFVLDGRVFSYQKGIAPEWESYGPGTVLLYELVRE
                     AFETGQREYNFLQGGEDYKSRWAKDGRALFTASIFNRTLKGVISNAGVRSKRLLKAAL
                     SRP"
     gene            1383..2531
                     /locus_tag="AC16BC_06315"
     CDS             1383..2531
                     /locus_tag="AC16BC_06315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3241271.1"
                     /translation="MDTLSLALFWSMVALITYTYVGYPLVILLLGTIIRRRVDRRPIE
                     PTVTFLITAYNEEKNIAQKLENTLSLDYPHEKLDVLVALDGSTDRTEDIVRGFADRGV
                     RVVRVDGRVGKTETQNQAVKHAKGEVIIFSDATTRYELGNIRAFVRNYADPTVGAVSG
                     CYSYHNPTGATIGVGNILFWKFENFIKTSQTRIRTLTGCCGCIYSVRRALYKPLPADI
                     ISDLVEPLKILESGYRIGFEPEAVAYEETTESASEEFRMRIRVISRGMRGLLMMRTLF
                     NPFRYGFVAFQLFSHKLLRWLVPIFLIVLLGASAALAHIPFYRVALVLQLAFYGCALV
                     GWMMEKMRLKMKVFAVPLYFLTVNIASLISLYKTLRGYKAVTWETVRR"
BASE COUNT          488 a          740 c          862 g          575 t
ORIGIN      
        1 ggagtacaag gcgcaatggg ccaaagcacg acgggtgttg ctgaccgcga ccgtctataa
       61 ccggacggtg cagggggccc tgtcaaaaac ggcgttccac tcgaagtcct ggctcaaggc
      121 cgtcggagag gttctggccg ccgagtccat cttgttctgg tttcagtagc ggtggtgggg
      181 cgagtgacgg catgtcgcat gcggcgtcgt gcacggattc gcgcggaggg gccgggttca
      241 tcgaccggcg ggtccgaggc gcgggggcaa gcgaggtgtt gacgtatacg gttcgactgg
      301 cgacggccgc tgaggtccgc gacgctcgcg atcgctggaa cgccttagtt ttgaaaatgc
      361 cgttgccgtc gttcttctgc acgtgggaat ggatttcgac gtggcaggag gtcttcggct
      421 cgcagtacga gccggtgatc ctcttcgtgt accgaggcga tgaactggcg ggcatcctgc
      481 ccttgtgccg tcgtgaagcg gtgctgaccc gcggtctgat gtcgggacgc atcctcagct
      541 attgcggggc tcgggagttg tacccggatc atttggatct gatcgcgccc gaggaagacg
      601 cggtcgcgtg tctgaacgcg atttgggcgt ttctggtcga ggagtattcg gcgtgggacg
      661 tcctccacct gtcgtatctg tggggcggtt ccggccttgt gtccgcgatc acgcgaccgg
      721 actttccgct ctcgcttcag acgactcagg cctcgttcgc gcgttatatc cacgtgtccg
      781 gcacattcga cgaattcttt gaccgatttg acaagaagtt ccgatacaat gtgcgcagtc
      841 gtcgcaaaaa attgatggaa gaacacggga tgcggtacat cacgtgcgac gcgggtcgcc
      901 agcccgaggc gctggatctg gtgttcgcgc tgaatgaccg ccggttcgcc agtaaaggcg
      961 tcggtagctc cctcatggcg ccatcgatca tcgactttca tcgtcggttg ctctcggctt
     1021 tggctggaac ggatcgaatt cagttgcgac tgcttggaaa cgatgaacag atcgccgcgg
     1081 catcatataa ctttgtgttg gacggccggg tgttctcgta tcagaagggg atcgcgccgg
     1141 agtgggagtc ctatggtccg ggtacggtgc tgctgtatga actcgttcgc gaggcgttcg
     1201 agaccggaca acgtgaatac aacttcttgc agggcggcga ggactacaaa agccgatggg
     1261 cgaaggacgg ccgggccctg tttaccgcgt caatcttcaa tcggacgctc aaaggggtga
     1321 tctccaacgc cggagtccgc tcgaagcggt tattgaaagc ggcgctgtcg aggccctgag
     1381 ccatggacac cctctcactc gcgttgttct ggtcgatggt cgccttgatc acgtacacgt
     1441 acgtgggcta cccgctcgtc attctgctgc tgggtacgat cattcgtcga cgggttgatc
     1501 gtcgcccgat cgagcccacg gtgacgtttc tgatcacggc ctacaacgag gaaaagaaca
     1561 tcgcccaaaa actggagaac acgctctcgc tggattatcc tcacgagaag ctcgacgtac
     1621 tggtggccct ggacggctcc acggaccgca ccgaggacat cgtcagagga ttcgcggatc
     1681 gcggcgtgcg ggtggtccgg gtcgacggcc gcgtcgggaa aaccgagacg cagaaccagg
     1741 ccgtcaaaca cgccaagggc gaggtgatca tcttctccga cgccacgacg cggtatgaac
     1801 tgggcaacat ccgggcgttc gtcaggaatt acgccgaccc cacggtgggc gcggtgagcg
     1861 ggtgttacag ctaccacaat ccgaccggcg cgaccatcgg cgtcggcaat attctctttt
     1921 ggaagttcga gaacttcatc aagacgtcgc agacgcggat tcggaccttg accggctgct
     1981 gcggatgcat ttattcggtc aggcgggcgc tgtacaagcc gctgcccgcg gatatcatca
     2041 gcgatctggt ggaacctctg aagatccttg agagcggcta tcggatcggg ttcgaacccg
     2101 aagccgtggc ctacgaggag accacggaga gcgcgtccga ggagttccgg atgcggattc
     2161 gcgtgatttc gcgcgggatg cgcgggctgc tgatgatgcg gacgctgttt aacccgtttc
     2221 ggtacggatt cgtcgcgttt caactgttct cccacaagct gctgcgctgg ctggtgccga
     2281 tctttttgat cgtgttgctg ggggccagcg cggcgctcgc gcatatcccg ttctaccggg
     2341 ttgcgttggt gctgcaactg gcgttctacg gctgtgcgct cgtgggctgg atgatggaaa
     2401 aaatgcgcct gaagatgaag gtcttcgcgg tcccgctcta ttttctgacc gtgaacatcg
     2461 catccctcat ctcgctgtat aaaacgctgc gcggctacaa ggccgtcacg tgggaaaccg
     2521 tgcgtcgatg aaaccgttcc gattcgtgat cattactcag gaggacccgt tctacgtccg
     2581 ggtatttttc gaggagttcc tgagccgtta cgcgccgcgc gacgagatcc aagccgtggt
     2641 gattgctccc accatgggca aacgc
//
LOCUS       DBNGTT010000282         2102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1977446, whole genome shotgun sequence.
ACCESSION   DBNGTT010000282 DBNGTT010000000
VERSION     DBNGTT010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1977446"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="AC16BC_06320"
     CDS             <1..338
                     /locus_tag="AC16BC_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR3241272.1"
                     /translation="WFGFAVALFRRPRRPTDRRLGAGTGTPGDVFMQIQFPLHSGQMA
                     GLTGRIVIAAVGVVTAMLSITGVVIWFRKRRARGLKRARRVSWARPLHAETGLQRTDA
                     PIPPILQRD"
     gene            433..900
                     /locus_tag="AC16BC_06325"
     CDS             433..900
                     /locus_tag="AC16BC_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241273.1"
                     /translation="MAHEDSRQCTRMNRCRQVNTGTRTGRRDDEERDGRFRADVGAVG
                     LVSGVRGPRHEGDQCEYKKSGARQKLLEQLPAEKEELFKRTMMEAREKKAAIREEIVK
                     ARELVEQEHRVMEDAVAKLAAQFTPEERKLLADALDRSKGHGHRGWSSHRRAS"
     gene            991..1572
                     /locus_tag="AC16BC_06330"
     CDS             991..1572
                     /locus_tag="AC16BC_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3241274.1"
                     /translation="MDDRVSELDDESLLRRIQEGDHGAFASLVHRHAKRFHAVAYRIV
                     HDRDDAEDLVQAAFLKLWERPDLWDQRKQTKFTTWFYTVVTNLGLDHAKRKRPIPLSD
                     EIELPDGGPNQDDLLDGKQRRAAVDRLVRQLPERQQLALNLCFYEDLSNQEAADIMGI
                     NLKALQSLIMRAKTTLKERLKDAPVKVTNGDER"
     gene            1562..1990
                     /locus_tag="AC16BC_06335"
     CDS             1562..1990
                     /locus_tag="AC16BC_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241275.1"
                     /translation="MNAEQFREALLIHGADVHRWPDEIRGRGVEALTRSIECRSLHEE
                     YARFEAALASRASEAPSADFAGRIISAARPRQRRGFRGLTELLASCFADLRLPAPVLT
                     VAAVLIVGLVVGLLLPAESPRGDSEATETLAFLDSTSEAL"
BASE COUNT          396 a          602 c          724 g          380 t
ORIGIN      
        1 gctggtttgg gttcgccgtg gctctatttc gacggccaag acggccgacc gatcggcgac
       61 tcggtgcggg gaccggcacg cccggcgatg tgttcatgca gatccagttc ccgcttcata
      121 gcggccagat ggcgggtctg accggacgca tcgtgatcgc ggccgtgggc gtcgtgaccg
      181 cgatgttgtc catcaccggg gtcgtgatct ggttcagaaa acgccgggcc cgcggtttga
      241 agcgtgcccg acgcgtgtca tgggcgcgcc ccctccatgc cgaaaccggg ctgcaacgga
      301 ctgacgcccc gattcctccc attctgcagc gagactgaag ctccccgaag attttccccg
      361 gctggttcgt atcacttccc gtcagtcggc aaaacgtcct gggaacacga gcgagtacgg
      421 agaaccgagc gaatggctca cgaagactcg cgtcagtgta ctcgtatgaa taggtgtcgg
      481 caggtcaaca cgggaacacg taccgggagg agagatgatg aagagaggga tggccgtttc
      541 cgcgctgatg tgggggctgt tggtctggtc tccggtgtac gcggaccccg gcacgagggg
      601 gaccaatgcg agtacaagaa gtccggcgct cggcagaagc tgctggaaca gttgccggcc
      661 gagaaggaag agctgttcaa gcggacgatg atggaagcca gagaaaagaa ggccgcgatc
      721 agggaggaga tcgtcaaggc gcgcgaattg gtcgagcagg aacatcgggt gatggaggat
      781 gccgtcgcga agctggccgc acagttcacg ccggaagaac ggaagttgct cgccgacgcg
      841 ctggacaggt ccaaggggca tgggcaccgt gggtggtcct cgcatcggcg cgcgtcgtaa
      901 ggcgatgccc ggcatccggg agcgcgacca tgcgctcccg gatgcacttg tggtataccg
      961 gatgccgaga caggggacgc cgcgacaacg atggatgatc gcgtctcaga gctggacgat
     1021 gagtcgttac ttcgccggat acaggaaggg gatcacgggg ccttcgcgtc gctggtccat
     1081 cgacacgcca agcggttcca cgcggtggcg taccggatcg tccatgatcg cgacgacgcg
     1141 gaagatctcg tccaggccgc gttcctcaag ctctgggaac ggcctgacct gtgggatcaa
     1201 cgcaagcaga cgaaatttac cacctggttt tacaccgtgg tcacgaacct gggtctggac
     1261 cacgcgaagc gcaagaggcc gattcccttg tccgacgaga tcgaattgcc ggacggaggg
     1321 ccgaaccagg acgacctctt ggacgggaag caacggcgcg cggcggtgga tcgccttgtc
     1381 cggcagttgc ccgaacgcca gcaactcgcg ctcaacctgt gtttctacga ggacctcagc
     1441 aaccaggaag cggcggacat catggggatc aatctcaagg cgcttcagtc gctcatcatg
     1501 cgcgcgaaga ccacgctgaa ggagcgattg aaagacgcgc cggtcaaggt gacgaatggc
     1561 gatgaacgct gaacaattcc gggaggcttt gctgatccac ggcgccgacg tgcatcggtg
     1621 gccggatgaa attcgcggtc gcggggtcga ggcgttgact cgctcgatcg agtgccggtc
     1681 gttgcatgaa gagtacgcgc ggttcgaggc ggccctcgct tccagggcgt ccgaggcgcc
     1741 cagcgcggat ttcgccggcc ggattatctc cgccgcgcgg ccgcgccagc gaagaggttt
     1801 ccgcggactc accgagttgc tggcgtcgtg ttttgcggat cttcggttgc ccgccccggt
     1861 cttgaccgtg gccgcggtgc tgatcgtggg gcttgtcgtc gggttgttgc tgcccgctga
     1921 gtcgccccga ggcgattccg aggcgaccga gactctggcg tttctcgact ccacgtcgga
     1981 ggcgttatga gcaggaacgt gaaaattgtg attgtgatct cgttggtcct caacgtgctg
     2041 cttgctggcg tgatcctggg gaacgtcgtc caccggatgg ggtggcgctc cgcgttcaaa
     2101 cc
//
LOCUS       DBNGTT010000283         2785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1466030, whole genome shotgun sequence.
ACCESSION   DBNGTT010000283 DBNGTT010000000
VERSION     DBNGTT010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1466030"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..411)
                     /gene="rplR"
                     /locus_tag="AC16BC_06340"
     CDS             complement(43..411)
                     /gene="rplR"
                     /locus_tag="AC16BC_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3241276.1"
                     /translation="MSTNSKARLDARQRRHRRVRKRVMGTKERLRLSVFRSNRYLYCQ
                     IIDDLEGRTVVSASTLEPSIQERAKGNSIDSAKVLGTTLAERAKAANVTTVVFDRGGY
                     LYHGRLKALADAAREAGLSF"
     gene            complement(459..995)
                     /gene="rplF"
                     /locus_tag="AC16BC_06345"
     CDS             complement(459..995)
                     /gene="rplF"
                     /locus_tag="AC16BC_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3241277.1"
                     /translation="MSRIGRKELEVPKGVDVKVVPGVVRVKGPKGELSKSIHPNVSVL
                     VENGRVRVSRASEDKADKALHGMVRNEIKNMVEGVSQGYERVLEITGVGFRAQVQGKK
                     LLMSLGYTHQIEFPLPAGVDAAVDKQTVVTLRGADKQLVGQTAAKIRSMREPEPYKGK
                     GVKYAGERIIRKEGKTGK"
     gene            complement(999..1397)
                     /gene="rpsH"
                     /locus_tag="AC16BC_06350"
     CDS             complement(999..1397)
                     /gene="rpsH"
                     /locus_tag="AC16BC_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3241278.1"
                     /translation="MSMTDPIADMLTRIRNAMMRRHESVLIPASRIKRDIAKLLKDEG
                     FVRDARVVSQEGRQAIKIDLKYLNEDDSVIVGIERVSTPGRRVYVGQDSIPTVKGGYG
                     VAILSTNKGILTDKQSRQAKVGGEVLCYVW"
     gene            complement(1462..1647)
                     /locus_tag="AC16BC_06355"
     CDS             complement(1462..1647)
                     /locus_tag="AC16BC_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR3241279.1"
                     /translation="MARTALINKSQQPQKFAVRQYHRCKSCGRVRGYIRKFAMCRICF
                     RNFSLRGDIPGVVKSSW"
     gene            complement(1702..2265)
                     /gene="rplE"
                     /locus_tag="AC16BC_06360"
     CDS             complement(1702..2265)
                     /gene="rplE"
                     /locus_tag="AC16BC_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3241280.1"
                     /translation="MAKTATTEVLPLRVRYQQEVVPLLMKEFTYGNPMQVPRVQKIVV
                     NVGMGEATSNAKLLEAAAGEIGQITGQRPLITKARKSIAGFKLREGMSIGCKVTMRGA
                     RMWEFLDRLLHVALPRIRDFRGVLGRSFDGRGNYTLGLKEQLIFPEIKYDEISAIHGM
                     DITIVTTAKNDAEAKALLKHLGMPFRN"
     gene            complement(2269..2619)
                     /gene="rplX"
                     /locus_tag="AC16BC_06365"
     CDS             complement(2269..2619)
                     /gene="rplX"
                     /locus_tag="AC16BC_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3241281.1"
                     /translation="MTLKTTEAAPTKARIKKGDTVMVLAGKERGKQGKVLHVMPAQSK
                     VVVEKLNLIKRHTKPSRENKGGIIEKEGALALGKVMVVCPQCSKPSRMGVAVLEGGRR
                     VRRCKQCNEIVDKG"
     gene            complement(2648..>2785)
                     /locus_tag="AC16BC_06370"
     CDS             complement(2648..>2785)
                     /locus_tag="AC16BC_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HIR3241282.1"
                     /translation="RFDGNSAVLITKENEPIGTRIFGPVARELRWKKFMKIISLAPEV
                     L"
BASE COUNT          456 a          973 c          755 g          601 t
ORIGIN      
        1 gccccctcga accatttgaa ctcttgaacc cttgaacctg atttaaaagc tcagccccgc
       61 ttcccgagcc gcgtcggcca gcgccttgag tcggccgtga tagagatagc ccccgcggtc
      121 gaacaccacg gtcgtgacgt tggcggcttt ggcgcgctcg gccaacgtcg tacccaacac
      181 cttggcggag tcgatcgaat tgcccttcgc acgctcctgg atcgacggct cgagcgtgga
      241 ggcggacaca acggtccgcc cctccaaatc atcgatgatc tggcaataca agtaacggtt
      301 gctgcgaaac accgacaacc gcaaacgctc cttggtgccc atgacgcgtt tgcgcacccg
      361 gcggtgccgg cgctgcctcg cgtccaacct ggccttcgaa ttcgtgctca tcgtctagat
      421 tccccgtcac atgccgcgcc cgcgcctgcc gggcggcgtt atttaccggt cttgccttcc
      481 ttgcgaataa tgcgttcgcc ggcgtacttc acgcccttgc ctttatacgg ttccggctcg
      541 cgcatcgagc gaatcttggc cgcggtctgc ccgaccagtt gcttgtccgc gccccgcaag
      601 gtcaccacgg tctgcttgtc caccgccgcg tcgaccccgg cgggcaacgg gaattcgatc
      661 tggtgggtgt accccaggct catgagcagt ttcttgccct gcacctgcgc gcggaacccc
      721 acgccggtga tctccaggac ccgctcatag ccctgcgaca cgccctcgac catgttcttg
      781 atctcgttgc gcaccatccc gtgcagggct ttatcggcct tgtcctccga cgcgcggctg
      841 acgcgcacgc gaccgttctc gaccagcacg gacacgttcg gatgaatcga tttggacaat
      901 tcgcccttcg gtcccttgac ccgcaccacc ccgggaacca ccttcacgtc cacgcccttg
      961 ggcacttcca gctctttacg tccgattcgc gacatcgatc accacacgta acacagcact
     1021 tcgcccccga ccttcgcctg gcgcgattgc ttgtccgtca gaatgccctt gttcgtcgag
     1081 aggatcgcca ctccgtaccc acccttgacc gtcgggatgc tgtcctggcc cacgtacacg
     1141 cgccgtcccg gcgtgctgac ccgctcgatc cccacgatca cggaatcgtc ctcgttgagg
     1201 tacttcaggt cgatcttgat cgcctggcgc ccttcctgcg acaccacccg cgcgtcgcgg
     1261 acgaatccct cgtccttgag cagcttggcg atgtcccgct tgatccgaga ggccgggatc
     1321 agcacggatt catgccgccg catcatggcg ttgcgaatgc gggtcaacat atctgcgatg
     1381 ggatcggtca tgctcatcga aacgtgactc cttcttgttc gccggtttcc cggttcgccg
     1441 gttccccggt gccgctcttc actaccagct cgacttgacc acgccgggga tatccccgcg
     1501 cagactgaaa ttgcggaaac agatgcggca catcgcgaac ttgcggatgt acccgcgcac
     1561 gcgcccgcag gacttgcacc gatggtactg ccggaccgcg aatttctgcg gttgctgaga
     1621 cttgttgatc aatgctgtac gcgccatagt ggactttctg attccgtcgt tatgtaatgt
     1681 tcgcggacgc ccgcttcccg tttaatttcg aaacggcatc cccaggtgtt tcagcagcgc
     1741 cttggcctcc gcgtcgttct tcgcggtcgt cacgatcgta atatccatgc cgtggatcgc
     1801 gctgatctcg tcgtatttga tctcggggaa aatcagctgc tccttgaggc cgagcgtgta
     1861 gttgccgcgc ccgtcgaacg accggccgag cacgccgcga aaatcgcgga tccgcgggag
     1921 cgcgacgtgg agcaggcgat ccaggaattc ccacatccgg gctccgcgca tggtgacctt
     1981 gcaaccgatc gacatgccct cgcgcagctt gaatccggcg atcgacttgc gggccttggt
     2041 gatcagcggg cgctggcccg tgatctgtcc gatctcaccg gccgccgctt ccagcaactt
     2101 ggcgttcgag gtcgcctcgc ccatgcccac gttcacgacg atcttctgca cccgcggcac
     2161 ctgcatcgga ttgccgtagg tgaactcctt catcaacagc ggcaccacct cctgctgata
     2221 ccgcacgcgg agggggagca cttcagtggt agccgtcttc gccatcggtt aacccttatc
     2281 cacgatctcg ttgcattgct tgcagcggcg aacccgacgc ccgccttcga gcacggcgac
     2341 gcccatccgc gaaggcttcg agcactgcgg acacacgacc atgaccttgc ccaacgccaa
     2401 cgccccctct ttttcgatga tcccgccctt gttctcgcgg gagggtttgg tgtggcgctt
     2461 gatgagattc aatttctcga ccacgacctt ggactgcgcc ggcatcacgt gcaacacctt
     2521 gccttgcttg ccgcgctcct tgccggccag caccatcacg gtgtcgccct tcttgatccg
     2581 ggccttcgtg ggagccgctt ccgtcgtctt taacgtcatc cgtatcatcg ttcgtccagt
     2641 gcgggcccta taacacttcg ggggccagcg aaatgatctt catgaacttt ttccaccgca
     2701 gctcgcgcgc caccgggccg aagatgcggg tcccgatcgg ctcgttctcc ttggtaatca
     2761 ggacggcgct gttcccgtcg aagcg
//
LOCUS       DBNGTT010000284         2677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1593787, whole genome shotgun sequence.
ACCESSION   DBNGTT010000284 DBNGTT010000000
VERSION     DBNGTT010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1593787"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..760)
                     /locus_tag="AC16BC_06375"
     CDS             complement(47..760)
                     /locus_tag="AC16BC_06375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3241283.1"
                     /translation="MHFRLKDKVALVTGGGRGIGRQIALAFAAEGAHVAIAARTEREI
                     KPVAERITGLGRRALAVPTDVADEARVAALVAETERAFGRIDILVNSAAVVLIEPLAK
                     TTAEQWRRTMDVNVTGAFFATRAVLPGMTQRNEGRIINISSTAGIEGFANFSAFCASK
                     FALIGLTRALAKELEGSPVTANALCPALVGTKRVAPPPKGSAPAPEPFSEIADVAVFL
                     AGDRGRAVSGAAIEISWRT"
     gene            complement(886..2040)
                     /locus_tag="AC16BC_06380"
     CDS             complement(886..2040)
                     /locus_tag="AC16BC_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3241284.1"
                     /translation="MNAVLNHERMIDYFMDLARIDSLSRREGAIARRLIADLESLGAK
                     VRIDDAGERVGGEIGNVIASVPGTAPGPWLLLSAHMDTVVPGEGVVPVRHGDRITSDG
                     RTILGGDDKSGLAIIMEVLRTLAERRLPHGGVEVAFTICEEMGLMGAKHLDANALTAR
                     DGLVLDSENATFLFTKGPAADKIEFTVTGLEAHSGIAPERGISAIQVMSQAIAAMRLG
                     RIDDETTANLGVMRAGAATNIVPKEATVAGEARSLDEAKLDAQTAHMRRCVHEAAERH
                     HVSVDGVTHRARVTERIERDFPRLDVRDDAPIVRAVLAAAGATGWSVTTRSTGGGCDA
                     NVFNHKGLRVANLGTGMRAIHTVNEYLLLDEFRRASEVVLETVRRHAGSK"
     gene            complement(2063..2485)
                     /locus_tag="AC16BC_06385"
     CDS             complement(2063..2485)
                     /locus_tag="AC16BC_06385"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3241285.1"
                     /translation="MTEPITYREGGPLDLDQLRGLLDQTPWARGRSKDALTRMLAHTD
                     FVFSAWDGPRMVAFARVLTDRIYRATLWDVVVDAAYQGRGVGEGLMSFVLAHPVLSRV
                     EKFWLNTRDKQKFYERFGFVSSPQGMYRERSRSNEAGT"
BASE COUNT          405 a          979 c          842 g          451 t
ORIGIN      
        1 ggacgcgcag cggcaacgca gcagatggcc cctgatgaac cggctcctac gtgcgccagg
       61 agatttcgat ggccgcaccc gaaaccgccc gtccccgatc tccggccaaa aacaccgcca
      121 cgtccgcgat ctcggaaaac ggttccggcg ccggggcgct tcccttgggc ggcggcgcga
      181 cgcgtttggt cccgaccaac gccgggcaca aggcgttcgc ggtcaccggg gagccttcga
      241 gttccttggc caacgcccgg gtcaggccga tcaacgcgaa cttggacgcg cagaacgcgg
      301 aaaagttcgc gaacccctcg atcccggccg tggacgagat attgatgatc cggccttcgt
      361 tgcgctgggt catgccgggg agcaccgcgc gggtcgcgaa aaacgcgccc gtgacgttca
      421 cgtccatggt gcggcgccac tgctcggccg tggtcttggc caggggttcg atgagcacca
      481 cggccgcgct gttcacgaga atgtcgatgc gtccgaaggc gcgctccgtc tccgcgacca
      541 acgccgccac ccgcgcctcg tccgccacgt cggtcggcac ggccagcgcg cgacgcccca
      601 gtccggtgat ccgttccgcc accggcttga tctcccgttc cgtccgggcg gcgatcgcca
      661 cgtgcgcgcc ctcggccgcg aacgccaggg cgatctgccg cccgatgccg cgccccccgc
      721 ccgtcaccaa cgccaccttg tccttgagtc gaaagtgcat tgcgagccct ttcagcgagc
      781 aacctgcaaa acgtgcttct ccacttcctg acgcttcgaa gcgtccctgc gccgcgcggc
      841 agggaccttc aatggccgtc cctccttgcg cgccctcccc cgtgatcact tcgatccggc
      901 gtgccgccgc acggtttcca acaccacctc gctcgcccgc ctgaattcgt cgagcaggag
      961 gtactcgttc acggtatgga tggcccgcat gcccgtgccc agattggcca cgcgcaggcc
     1021 cttgtgattg aacacgttcg cgtcgcagcc gcctcctgtc gagcgcgtgg tcaccgacca
     1081 ccccgtcgcg ccggccgcgg ccaacaccgc gcggacgatc ggcgcgtcgt cccgcacgtc
     1141 gagccgggga aagtcgcgtt cgatccgctc cgtcacccgc gcgcgatgcg tcaccccgtc
     1201 cacgctcacg tgatggcgct cggccgcctc gtgtacgcag cgccgcatgt gcgcggtttg
     1261 cgcgtccaac ttggcctcgt ccaggctgcg cgcctcgccc gccaccgtgg cttccttcgg
     1321 cacgatgttg gtggcggcgc ccgcgcgcat cacgcccagg ttcgcggtgg tctcgtcgtc
     1381 gatgcggccc agccgcatgg ccgcgatcgc ctggctcatc acctggatcg cggaaatgcc
     1441 ccgctcgggc gcgatcccgg agtgggcttc cagtcccgtc acggtgaatt cgatcttgtc
     1501 cgcggccggc cctttggtga acagaaaggt cgcgttctcg gagtccagca ccagtccgtc
     1561 gcgcgcggtg agcgcgttcg cgtcgaggtg tttggcgccc atgagcccca tctcttcgca
     1621 aatcgtgaac gcgacttcga ccccgccgtg cggcaagcgg cgttccgcca gagtgcggag
     1681 gacttccatg atgatggcca gtccggactt gtcgtccccg cccaggatcg tgcggccgtc
     1741 gctcgtgatg cggtcgccat gtcgcaccgg caccacgcct tcgcccggca ccacggtgtc
     1801 catgtgcgcg gagagcagca gccacggccc gggggctgtt ccgggaaccg aggcaatcac
     1861 gttcccgatc tcgccgccga cgcgttcccc cgcgtcgtcg atgcggacct tggcgcccag
     1921 cgactccagg tcggcaatca gccggcgggc gatcgcgcct tcgcggcgcg acaagctgtc
     1981 gatccgggcc aggtccatga agtagtcgat catccgttcg tggttcagta ccgcgttcat
     2041 cgccgtcagc ctccgggcct cgtcatgtcc cggcctcgtt ggagcgagag cgttcccggt
     2101 acatgccctg agggctggat acgaagccga atcgttcgta gaatttctgt ttgtcccggg
     2161 tgttgagcca gaatttttcg acgcgcgaga gcacggggtg cgccagcacg aacgacatca
     2221 acccctcgcc cacgccgcgg ccctggtagg ccgcgtccac caccacgtcc cagagcgtgg
     2281 cgcggtaaat ccggtccgtg agcacgcgcg cgaacgccac catgcgcgga ccgtcccacg
     2341 cgctgaacac aaaatccgta tgcgcgagca tgcgcgtcaa tgcgtccttc gagcggccgc
     2401 gcgcccaggg cgtctggtcc aacaatcccc tgagttgatc gaggtccagg ggaccgcctt
     2461 ctcgatacgt gatgggttcc gtcacagccg tccatgccgt ttgcgcgtgg agttactcta
     2521 gcgaacggct gccggaaaca caagcgcgag cacgatccct cagggcacgg gatcgccgtc
     2581 atcggacgga tgggaccgat cggtctgatc gggcgtctcc gacgacgcgg actcctcggt
     2641 cggcacgcga tacgcgccgg agagccaccg gcccatg
//
LOCUS       DBNGTT010000285         6370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1593799, whole genome shotgun sequence.
ACCESSION   DBNGTT010000285 DBNGTT010000000
VERSION     DBNGTT010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1593799"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /gene="rho"
                     /locus_tag="AC16BC_06390"
     CDS             <1..544
                     /gene="rho"
                     /locus_tag="AC16BC_06390"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3241286.1"
                     /translation="HVQVAEMVIEKAKRLVEHKKDVLILLDSITRLARAYNSVVPPSG
                     KVLSGGVDSNALQRPKRFFGAARNIENGGSLTIMATALIDTGSRMDDVIFEEFKGTGN
                     MELHLDRKLADKRIFPAIDINLSGTRKEELLVERDDLNKMWILRKVLSPLSTVESMEF
                     LLDKIRATKTNREFLESMNK"
     gene            745..2172
                     /gene="hemG"
                     /locus_tag="AC16BC_06395"
     CDS             745..2172
                     /gene="hemG"
                     /locus_tag="AC16BC_06395"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIR3241287.1"
                     /translation="MLVIGAGVSGLAAAYGLARAGCRVTVLEQSSRVGGCVHSERTPE
                     GYLVENGPNSLLNLSPAVDRLCRELGLEDQRLFQQAFSRRRYLVKDRALVPVPSRVGD
                     FPRSPLWGAWAKLRALAEPLIPASRSQSDESVAAFVRRRFGAEMVDYGVEPFVAGIFG
                     GNPDDLSMSSSFPRLVALEREYGSVVWGTLRTRMKQGRARRPLQVFSFRGGVGRLPDA
                     LRTALGDRVVTGARVLGVRREVRDGIPRFFARVDRGGQTRELSADRLIVATPADTAAR
                     LLADLSPPLADQLEQIPYAPVGLVHLGVRRDACSRLPEGSGCLVPKREGLPILGSLWS
                     SNVYPDRAPEGRVLLTNYVGGMRDHDVLKWTDDELVGRTAEALRGLIGLAGQPEFVRV
                     VRHPRAIPQYLIGHAARVAAIDRLLAPHAGLHLAGNYLRGVSVRDCLTHGLELADRIA
                     RQTAQPGATPHVGFSGSETLVGTSS"
     gene            2445..3260
                     /locus_tag="AC16BC_06400"
     CDS             2445..3260
                     /locus_tag="AC16BC_06400"
                     /inference="COORDINATES: protein motif:HMM:NF026037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-sandwich domain-containing protein"
                     /protein_id="HIR3241288.1"
                     /translation="MKLSRALVGVAVGAMLAFGSAAMAEYTEAPVADGGTVSGKITFK
                     GAVPGPKTAAFAKFPNPGYCEKTDSDGKGNRAVQEVKVGKDGGLQDVVVYIENVEKGK
                     PFKFEGTDVKIEGCKFLVQGGPSIMTGVVMKKKEIRVLNEDADPTDPKAATGVLHNPH
                     SYEVAGANNTTIFNLPLPEKGQTIKKPVILRKKDSTFMIECDQHNYMQVFFMPIENPY
                     YAVVGEDGSFSISDVPPGTYEVYAWHPKLGKQEANITVTAKGTAKADFAFAAK"
     gene            3453..4382
                     /locus_tag="AC16BC_06405"
     CDS             3453..4382
                     /locus_tag="AC16BC_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3241289.1"
                     /translation="MDEKPESRAKIPMKKDALFYGLVAAFVAFVLMIVFMLYAMVQSK
                     AVMRRAAQTQSAPAEAVKTEEWDFSRLQIAKQEDGSEMVLVPAGPFLMGSPPVEGDQD
                     EMPQRGVFVGAFWIDLYEVTFVQYDLFMRATQAPKPVIPVLQDDPAKLMAPEQPVVAV
                     SWTQASDYCTWLGKRLPTEAEWEKAARGDHAVAWPWGNAFGPGLANVQGGEDGFVYTA
                     PPGSFPKGRSPYGVYDMSGNVSEWVADWYDHKYYLGAPFESPKGPETGKHRVYRGGSW
                     NDTPSDVRTAKRFAAAPHQTSAVIGFRCARNAD"
     gene            complement(4455..4592)
                     /locus_tag="AC16BC_06410"
     CDS             complement(4455..4592)
                     /locus_tag="AC16BC_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241290.1"
                     /translation="MDATSETPDEQTARRRSIFDWIVLLGMVINIVIAAFMVLYYFDL
                     F"
     gene            4958..5170
                     /locus_tag="AC16BC_06415"
                     /pseudo
     CDS             4958..5170
                     /locus_tag="AC16BC_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019325.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5237..>6370
                     /locus_tag="AC16BC_06420"
     CDS             5237..>6370
                     /locus_tag="AC16BC_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HIR3241291.1"
                     /translation="MGIAIGPDLSVENFSVTGTGKLSISSRARNRGNATAAASEMSFY
                     LSADAAYQAGTDQSLVDLAGNPCKRGVGGVAAGGYDPASGTVSTTCYVPGNVAPGFYY
                     FIAFVDSGSVVTETDEGNNIAVYGTVSVGPGTFIESINPNCGKVGDVVKIFGTGFGST
                     KGTSTVTFNGITATNTLFWSETGTEIHVQVPVGATTGPVRVIRNGGSNAVTFTVSDTC
                     TLSGPADYDIVAVHAPGSMLVGEAQYLDIFVKNINAGSAPGFGYSITGITTGTAEVVL
                     NGSGSGNAALGETVKIGVLFTAPNKPGQCVDFTVLLTDQDPDLDEAVMRTCLEPDYLF
                     AGFLPPVTINGKAFKIGSTIPVKFRLIDRETGAYLDNKDAFLWV"
BASE COUNT         1166 a         1801 c         2194 g         1209 t
ORIGIN      
        1 ccacgtgcag gtcgccgaga tggtgatcga gaaggccaag cgcctcgtcg agcacaagaa
       61 ggacgtgctg atcctgctcg actcgatcac ccgcctcgcg cgcgcctaca acagcgtcgt
      121 gccgccgtcg ggcaaggtgc tgtcgggcgg cgtggactcc aacgcgctcc agcggcccaa
      181 gcggttcttc ggcgcggccc gcaacatcga gaacggcgga agcctgacca tcatggcgac
      241 cgcgctcatc gacaccggca gccggatgga cgacgtgatt ttcgaagagt tcaagggcac
      301 cggcaacatg gagttgcatc tggaccgcaa actcgccgac aaacgcatct tcccggcgat
      361 cgacatcaac ttgtcgggga cccgcaagga agagttgctg gtggagcggg acgatctgaa
      421 caagatgtgg atcctccgca aggtgctcag cccgttgagc acggtggaga gcatggagtt
      481 tctgctcgac aagatccgcg ccaccaaaac caatcgcgag tttttggagt ccatgaacaa
      541 gtagttcgga ccctcgagtc cgagccagtc ttgactttcc gtctcgattc ctttctaatc
      601 ctcgtgcctc actcgccccg cggcgggtga cgcacactgt ttcccgtctg cggtccgtcc
      661 cgggggagcc gtgttcttcc tccgcgcccg gcttccggcg cggagttcgg caaggggcgt
      721 agaaccgtgc aatccgagaa ccatgtgttg gtcatcggcg ccggcgtgtc cggcttggcg
      781 gcggcgtacg gcttggcgcg cgccggttgc cgcgtgacgg tcctcgaaca gtcctcccgg
      841 gtcggcggct gcgttcacag cgagcgcacg ccggaagggt atctggtcga aaacgggccg
      901 aacagcctgc tcaacctaag tcccgcggtg gaccggctct gccgcgaatt gggtctggaa
      961 gaccagcgtc tgttccagca agccttttcc cgccggcggt atctcgtcaa agatcgcgcg
     1021 ctggttccgg ttccctcgcg agtgggggac ttcccgcgct cgccgttgtg gggcgcgtgg
     1081 gccaaactgc gcgcgctcgc cgaacccttg atccccgcgt ccaggtcgca atcggacgaa
     1141 agcgtggcgg cgttcgttcg ccggcgcttc ggcgcggaga tggtggatta cggcgtggaa
     1201 ccgttcgtgg ccgggatttt cggcgggaac cccgacgatc tgtccatgtc gagcagcttt
     1261 ccccgactcg tggcgctgga acgcgaatac ggcagcgtgg tgtgggggac gctccgcacg
     1321 aggatgaagc aaggccgcgc gcggcgtccg ctccaggtgt tttcgttccg gggcggcgtc
     1381 ggacgcttgc ccgatgcgct gcgcaccgcg ctgggcgatc gggtcgtgac cggggcgcgg
     1441 gtgctcggcg tgcgtcgcga ggtccgcgac ggcatcccgc gcttcttcgc gcgggttgac
     1501 cgcggaggcc aaacgcgcga actgagcgcc gaccgtctca tcgtcgccac gccggccgat
     1561 accgcggcca gactgctcgc cgacctgtct ccgccgctgg ccgatcaact cgaacagatt
     1621 ccgtacgcgc cggtcgggtt ggtccatctg ggcgtgcggc gggacgcgtg ttcgcggctg
     1681 ccggaaggct ccgggtgtct ggtgccgaag cgcgaggggt tgccgatcct gggatcgttg
     1741 tggagttcca acgtgtaccc ggatcgcgcg ccggaggggc gggtgctgct gaccaattac
     1801 gtgggcggca tgcgcgatca cgacgtgctg aagtggaccg acgacgagct ggtggggcgg
     1861 acggcggagg ctctgcgcgg tctgatcggg ctcgcgggcc agccggagtt cgtccgggtg
     1921 gttcgtcatc cccgcgcgat cccgcagtat ttgatcggtc atgccgcgcg cgtggccgcc
     1981 atcgatcggt tgttggcgcc gcacgccggg cttcacctcg ccggcaacta tttgcgcggc
     2041 gtgtcggttc gcgactgtct cacccacggt ctcgaactcg cggaccgcat cgcccgccag
     2101 accgcccagc ccggggcgac tcctcacgtc ggtttttcag ggtccgagac gctggtcggg
     2161 acgtccagct gaccaacgga acgagtgtcc agaatcgacg atttgacctt gacatcgtcg
     2221 ttgctttggg tttatagttc gccccgtttt tttgggtgtc tcgttcaatc gctcagcatg
     2281 aggcagttcc caccgggcaa tgagtaatcc ctgacgggtg aatagggcgg cgccgcagtc
     2341 gtctaagtgg ttgcggggtg agtatgcgac gcaacgacgg gcggtcggcg gctgcaatga
     2401 ggcgtgcagc gagacgtggt cggtttttca acgggagggg gcaaatgaaa ctcagcaggg
     2461 cattggtcgg tgtggcggtc ggggcgatgt tggcgttcgg gtcggcggcg atggccgaat
     2521 atacggaagc gccggtggct gatggcggaa cggtgagcgg gaagattacg ttcaagggcg
     2581 cggttcccgg tccgaagacc gctgcgttcg caaagttccc gaacccgggt tactgcgaga
     2641 agactgattc ggacggcaag ggcaaccgcg cggtccaaga ggtgaaggtc ggcaaggacg
     2701 gcgggttgca ggacgtcgtg gtctacatcg aaaacgtgga aaaaggcaaa ccgttcaagt
     2761 tcgagggcac cgacgtgaag atcgaagggt gcaagttcct ggtgcagggc ggaccgtcga
     2821 tcatgaccgg cgtggtcatg aagaagaagg agatccgggt gctgaacgag gacgcggatc
     2881 cgaccgatcc caaggccgcg accggcgtgc tgcacaaccc gcacagctac gaagtggccg
     2941 gcgcgaacaa cacgaccatc ttcaacctgc cgttgccgga gaaggggcag accatcaaga
     3001 agcccgtgat cctgcgcaag aaggacagca ccttcatgat cgagtgcgat cagcacaact
     3061 acatgcaggt gtttttcatg ccgatcgaga atccgtacta tgcagtcgtg ggcgaggacg
     3121 gatcgttctc gatcagcgac gtgccgcccg ggacctacga agtgtacgcg tggcatccca
     3181 agctgggcaa gcaggaggcg aatatcacgg tgaccgcgaa ggggacggcc aaagccgact
     3241 tcgcgtttgc ggcgaaataa cgcgagtcat tgcggcgact cgatcgagcg cgcggcgctc
     3301 gtcggcgccc aaactcggaa tggtaatcgg acatggcggg gtcttccccg ccatgtctgt
     3361 ttttcgggcg tgatcatccg gccggcgctg ttcggcgggc atggccgcga aggcggacgc
     3421 gggtgacgtg atgacgggcg gaaaggtatg ccatggatga gaagccggag tcgcgggcga
     3481 agatcccgat gaagaaggac gccctgttct atgggctggt cgcggcgttc gtggcttttg
     3541 tcttgatgat cgtgttcatg ttgtacgcga tggttcagtc caaggcggtc atgcgacggg
     3601 ccgcgcaaac ccagtccgct ccggccgaag cggtcaaaac cgaagagtgg gacttcagcc
     3661 ggcttcagat cgccaaacaa gaggacggtt ccgagatggt gttggttccg gccggtccgt
     3721 tcttgatggg gagtccgccg gtcgagggtg atcaggacga gatgccgcaa cgcggcgtgt
     3781 tcgtgggcgc gttctggatc gacctgtacg aggtcacgtt cgtccagtac gacctgttca
     3841 tgcgcgccac ccaggctccc aagccggtga tcccggtgtt gcaggacgat cccgccaaac
     3901 tcatggcgcc cgagcagccg gtggtcgcgg tgtcgtggac ccaagcgagc gactattgca
     3961 cgtggctggg caagcggctc ccgaccgaag ccgagtggga gaaggccgcg cgcggcgatc
     4021 acgcggtggc ctggccgtgg ggcaacgcgt tcgggccggg tcttgcgaac gtccagggcg
     4081 gcgaagacgg gttcgtgtac acggcgccgc cgggctcgtt cccgaagggg cgaagccctt
     4141 acggcgtcta cgatatgtcg ggcaatgtgt cggagtgggt ggccgactgg tacgaccaca
     4201 agtactactt gggggccccg tttgaatcac ccaaaggtcc ggaaaccggg aaacaccggg
     4261 tctaccgcgg tggatcgtgg aacgacacgc cgtccgacgt gcggaccgcc aaacggtttg
     4321 ccgccgctcc ccaccagacc agcgcggtga tcggatttcg atgcgcgcgc aacgcggact
     4381 gatccgcgac gcgtgggtaa actcagtcgc gaatgcgaag acgctgaatc gtgtccggtt
     4441 ggcctgaggc ggcgttagaa tagatcgaag tagtacagaa ccatgaacgc cgcgatgact
     4501 atgttgatca ccatgcccaa taagacgatc caatcgaaaa tggaccgtct tcgcgcggtc
     4561 tgttcgtcgg gcgtttcgga tgtcgcgtcc attccctgcc tcgcattgtc gccatgatca
     4621 gcacgaaaca caatgtccgc gacaggatgc caaattccgc gcgggcgcgt caagcggaga
     4681 cggacgcgcg tgtctttgct cggaaagtat tcttgacttg atgtttgttt acggtcgtgc
     4741 tatctctggg cctcctacgc ctactttttc atccattatt tctcacataa ggagaaacgc
     4801 gatgaagagt tcacggaccc tgtggcagca cgtgtcggtt tattcaatcg tagggttcgt
     4861 cctagccgtg ttgtcgttga ttgcgccttc atcggcgttt gcaggaccgg acctgagcgt
     4921 ggagaacttt agcgtgacgg gcggaacggt gctgcggatg gtggagcggg tgcgcaacaa
     4981 cgggaatgcg ccggcggggg cgaccgcggt gacgttctac gcgtccacgg atgggacgtt
     5041 ggacgcgggg gacgtggtgg tgtgcacgcg gaccgtggca agcgtggcgg cgggggcgta
     5101 cgatccggcc agcgggaccg cgacgacgga ctgcacgaac agcacggtgg cgccgggggt
     5161 gtatacgatc atcgcgaagg tggacgtggc ggatgtggtg agcgagagca acgaggccaa
     5221 caacacgaag gtcgtggtgg gcattgcgat cggaccggac ctgagcgtgg agaactttag
     5281 cgtgacggga actgggaagc tttcaatcag tagtcgcgcg cgcaaccggg gcaatgcgac
     5341 cgctgccgca tctgagatga gtttttacct ttcggccgat gccgcgtatc aggcggggac
     5401 ggaccagtcg ttggttgatt tggccgggaa cccgtgcaaa cgaggcgttg ggggggtagc
     5461 cgccggaggg tatgatccgg ccagtgggac ggtgtctacc acgtgctacg tgccgggaaa
     5521 cgtggcgccg ggattctatt acttcatcgc gttcgttgat tcagggagcg ttgtgaccga
     5581 aaccgacgag ggcaacaaca tcgctgtgta tggaacggtt tcggtcggtc ctggaacgtt
     5641 catcgaatcg atcaacccca actgcgggaa ggtcggcgat gtcgtgaaga tttttgggac
     5701 cggctttgga tcgaccaaag gcacgagcac ggtgaccttt aatggaatta ctgcgaccaa
     5761 cacgttgttc tggtccgaga ccgggaccga aattcacgtt caggttccgg tgggcgcgac
     5821 tactgggcct gtgcgcgtca tccggaacgg cggcagcaac gcggtgacgt tcacggtgtc
     5881 ggatacctgc acgctctcgg ggcctgcgga ctatgacatt gttgcggttc acgcgccggg
     5941 atccatgctg gttggcgaag cgcagtacct cgatatcttt gtgaagaata tcaatgccgg
     6001 ttcggccccc ggattcgggt acagcatcac cgggatcacg accggcacgg cggaagtggt
     6061 gttgaacggg tccggttctg gaaatgccgc gctgggcgag acggtaaaga tcggagtgct
     6121 cttcacggct ccgaacaagc ccggtcaatg tgtggatttc acggtgttac tgacggatca
     6181 agatcctgat ctcgatgagg cggtcatgag gacgtgttta gagcccgatt acctcttcgc
     6241 gggattcctt cctccggtga cgattaatgg gaaggcgttt aagatcggca gcaccattcc
     6301 ggtgaagttc cgattaattg atcgggaaac aggggcgtac cttgataaca aagacgcgtt
     6361 tctctgggtt
//
LOCUS       DBNGTT010000286         1789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1849699, whole genome shotgun sequence.
ACCESSION   DBNGTT010000286 DBNGTT010000000
VERSION     DBNGTT010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1849699"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1789
                     /gene="topA"
                     /locus_tag="AC16BC_06425"
     CDS             <1..>1789
                     /gene="topA"
                     /locus_tag="AC16BC_06425"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR3241292.1"
                     /translation="PDREGEAIAWHIAEEFNGAKGKVKRVLFHEITERAVKQALEHPV
                     ELNLRKVDAQQARRILDRIVGYSISPLLWDKVRRGLSAGRVQSVAVRMICEREREIAA
                     FRSEEYWSITALLEGGGPPAFEAKLVKWRGEDVVLSTADATNAVAEAIRAQPFTVQQV
                     EKKERRKNAPAPYTTSKLQQEASRKLRFTPKRTMMLAQRLYEGVELGSEGPVGLITYM
                     RTDSTRVSAEAQQETTALVRERFGDDYVPQTPNVYRNKKDAQDAHEAIRPTSVTRDPE
                     QLRAVLDRDLYQLYAMIWKRFVASQMTPAVLDQTKVDIAAGEAVFRATGSVIRFPGFT
                     ALYTESTEATAATKADDEDEERRLPPVQQGDRLSLRELTPKQHFTQPPPRFNEAMLIK
                     ELEEQGIGRPSTYASIISTIQDRKYVAKVEGRLKPTELGVVVNDLLVQHFPSIVNVEF
                     TAKMEEDLDQIEEHDKSWVETVREFYGPFAGHLSKATHEMRDVKREEQPTDIVCERCG
                     KNMVIKWGRNGRFLACPGYPECKNTKEFRTEADGTIVIVPKQTETNEICDKCGGAMVI
                     KTGRFGRFIACSNYPACKTTRSLGTGVTCP"
BASE COUNT          385 a          558 c          574 g          272 t
ORIGIN      
        1 cccgaccgcg agggcgaggc catcgcgtgg cacatcgccg aagagttcaa cggcgccaag
       61 ggtaaggtga aacgcgtgct gtttcacgaa atcaccgagc gcgcggtcaa gcaggcgctg
      121 gaacacccgg tcgaattgaa tctcaggaag gtcgacgccc agcaggcgcg ccggattctg
      181 gatcgcatcg tggggtattc gatcagcccc ttgttgtggg acaaggtgcg tcgcgggctc
      241 tccgcgggtc gggtgcagtc ggtcgcggtc cgcatgatct gcgaacgcga acgcgagatc
      301 gcggcgtttc gcagcgaaga atactggtcc atcaccgcgt tgctcgaagg cggcggaccg
      361 cccgcgttcg aagccaaact cgtgaagtgg cgcggcgaag acgtcgtcct gtcgaccgcc
      421 gacgccacca acgccgtggc ggaagcgatc cgcgctcagc cgtttacggt ccagcaggtt
      481 gagaagaagg agcggcgaaa gaacgcgccc gcgccctaca ccaccagcaa actccaacag
      541 gaagcgtcgc gcaagctccg tttcacgccc aaacgcacca tgatgttggc ccagcgcctg
      601 tacgaaggcg tggagctggg cagcgagggg ccggtcgggc tcatcaccta catgcgaacc
      661 gactccaccc gcgtgtccgc cgaggcccaa caggaaacca ccgcgttggt gcgcgagcga
      721 ttcggcgatg attacgtgcc gcagacgccg aacgtgtacc gcaataagaa ggacgcgcag
      781 gacgcgcatg aggcgatccg ccccaccagc gtgacgcggg atccggaaca actgcgagcc
      841 gtcttggatc gcgacctgta tcaactctac gccatgatct ggaagcgctt cgtggccagc
      901 cagatgacgc cggccgtgtt ggatcagacc aaggtggaca tcgccgcggg cgaggccgtg
      961 tttcgcgcca ccggctcggt gatccggttt ccgggattta ccgcgttgta caccgaatct
     1021 acagaagcca ccgcggccac caaggccgat gacgaagacg aggaacgccg cctgccgccc
     1081 gtgcagcaag gcgatcggtt gtcgctgcgc gaactcacgc cgaagcagca ctttacccag
     1141 ccgccgcccc gcttcaacga agccatgttg atcaaggagc tggaggagca gggcatcggg
     1201 cgcccgagca cgtacgcctc catcatctcg accatccaag atcgcaaata cgtggccaag
     1261 gtggagggcc gtctcaagcc cacggaattg ggggtggtgg tcaacgacct gctggtccaa
     1321 cactttccat cgatcgtgaa cgtcgagttc accgcgaaga tggaagagga tcttgatcag
     1381 atcgaagagc acgacaagtc ctgggtcgag accgtgcgcg aattttacgg cccgtttgcc
     1441 ggccatctct cgaaggcgac ccacgaaatg cgggacgtca aacgcgagga acagcccacg
     1501 gacatcgtgt gcgagcggtg cggcaagaat atggtgatca agtggggccg caacggccgg
     1561 ttcctggcct gccccggcta tcctgagtgc aagaacacca aagaatttcg cacggaagcg
     1621 gacggcacga tcgtgatcgt gcccaaacag accgaaacca atgagatctg cgacaagtgc
     1681 gggggcgcaa tggtgatcaa gaccggccgg ttcgggcggt tcatcgcgtg ctcgaactac
     1741 ccggcgtgca agaccacgcg ctccctgggc accggggtca cgtgcccgc
//
LOCUS       DBNGTT010000287         5483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_829390, whole genome shotgun sequence.
ACCESSION   DBNGTT010000287 DBNGTT010000000
VERSION     DBNGTT010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_829390"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="AC16BC_06430"
     CDS             <1..604
                     /locus_tag="AC16BC_06430"
                     /inference="COORDINATES: protein motif:HMM:NF042645.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HIR3241293.1"
                     /translation="VWEPAHGRVWRPRVDGDWRPYWRGHWSWRDDWVWVSADPWGDVP
                     FHYGDWAWSGRFGWVWIPGAVWSPARVTWIVDGPVIAWAPAGLQITIGSAPRFWVYAD
                     REHFTRGIVRPHRLPPPRGHFHGRTVIRDVDRVFVPNRPVGRVLREEPRRERTVTVGG
                     ERRLVSPRDDRPNRGFRFESTRGSGRGMGAARGFERRDAR"
     gene            744..1067
                     /locus_tag="AC16BC_06435"
     CDS             744..1067
                     /locus_tag="AC16BC_06435"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3241294.1"
                     /translation="MDQSDRRSAGRIPHLVEIRYSSDSPVLSARVTDVSEKGLFVEAR
                     SPLPEGSMVSFSFLLTNTPWDKPITGHGKVVWRQEAVGMGIEFTDLSDENRSKLKIFV
                     GNQAA"
     gene            complement(1097..2104)
                     /locus_tag="AC16BC_06440"
     CDS             complement(1097..2104)
                     /locus_tag="AC16BC_06440"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3241295.1"
                     /translation="MSSPPRSQESDPVQEALARFLNAFPEGPSAVEHVTLDEALDRVA
                     AEPIAATLDSPPFARAIVEGFLVNADDTAASNAGPVSFTIAGTIRPGDAAPKQIAPGT
                     AWEVFTGSAVPAGACGVIRAWDAKRTGQRVTCSSPVQAAANIEAQGCDLTRGAVVVAK
                     GAQIGPDEMSLIAAQGIDTVPVAARPIVGIFGSGNEVIPHTARLTPGAIWDCNTPALS
                     ALIRREGGIPKRYGVIRDDFDTFRRAVSDALPHCGMIVIAGGTAVDGREFVRDLVAAV
                     GSPGVLVNGVPMRSGKPLIMGVAGQVPIVCVAGHPPEALRGFRLFGAPALSRLLGRIR
                     P"
     gene            complement(2108..3496)
                     /locus_tag="AC16BC_06445"
     CDS             complement(2108..3496)
                     /locus_tag="AC16BC_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3241296.1"
                     /translation="MSHPQEGYGIVVVGAGPAGMSAAAQFAKNGHRVVILNRDCKVGG
                     LAEYGIFPSKHRLRTGLQKTYREILSNPHVLYFGNTSVGRDRELTLDELRALGADAIV
                     FSTGAQGTKTIGVEGDNAAGVFHAKDVVFHYNQLPGYSERPFDVGRRVCVIGIGDVMI
                     DIAHWLIRYKKVQEVTAIVRRGPAERKYSPKETHAVCANIDKDALSREFARIRERLEA
                     VGQNPDQILADMVGEFKKPDPPVSGTMMKFRFLSSPRKVLVDAQNRVRALEVENTRLE
                     KKGDDTAAKGTGTADEIPCDSVIFAVGDCVDHAVGLPYKSGMFVTNPTPSGNEPDDAW
                     FQAYDEASGKIVEGVFVAGWARKASEGLVGIAKRDGEWCAEVVHRYLDGRPTRCASKL
                     DEFCGRLKTLIDSRQPDVVNKEDLQLLAQVEREEAAKSGVEEFKYATNQEILAAIRRR
                     RSMVGGREFSPA"
     gene            complement(3760..4875)
                     /locus_tag="AC16BC_06450"
     CDS             complement(3760..4875)
                     /locus_tag="AC16BC_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR3241297.1"
                     /translation="MGALVRALAVQGDSIWIGTTNGVMKFHIPTEQHKVYTTKDGLLS
                     NVVLGITIAPDNAAWIGTYGGGLTRFDGTNRWTTYTPYGAGATGDYRNPCTANSSIPP
                     LKKGGKGGFTPCAGSPADNGRYQRYQRGDGIGDLWVYQTMFLPDGTMWAATWKGASRF
                     NGTTFTTYSTGDGLVDKWVYSMAVDRDKRFWFGTEGGVTMYDGKRWTSWTHKDGLGAE
                     LPRASGEPKGKAPSPHHEQYDKDISDYNPNYVLATAIDAGQNKWFGTWGGGLSRFDGK
                     QWITYTTRDGLAGNVVNALVFDRRGVLWIGTDAGVSRLDPAACTAASSGRKPPCKFTT
                     FTTFDGLFHDAVYAVAIDSTGAKWFGTYGGVSRYTGP"
     gene            complement(5142..>5483)
                     /gene="crcB"
                     /locus_tag="AC16BC_06455"
     CDS             complement(5142..>5483)
                     /gene="crcB"
                     /locus_tag="AC16BC_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR3241298.1"
                     /translation="GANARFIVGSWVGSTFGARVPLGTFVVNVSGCFLIGIVGTVVVE
                     RLVARPEHVQYLLAIGFLGAYTTFSSFAWENHVLFLEGRWLTAALYMALSVFAGLISV
                     QLGVFLAKAWL"
BASE COUNT          923 a         1751 c         1824 g          985 t
ORIGIN      
        1 ggtgtgggag cccgcgcacg gccgagtctg gcggcctcgc gtggatggcg actggcggcc
       61 gtattggcgc gggcactgga gttggcgcga cgactgggtc tgggtatccg ctgatccctg
      121 gggcgacgtg ccgtttcact acggcgactg ggcgtggtcg gggcgcttcg gctgggtctg
      181 gattcccggc gcggtgtggt cgcccgctcg ggtcacgtgg atcgtggacg gaccggtcat
      241 cgcgtgggcg ccggccggct tgcagatcac gatcggctcc gcgccgcgat tctgggtgta
      301 cgccgaccgc gaacacttca ctcgcggcat cgtccgcccg caccgcctgc ctccgccgcg
      361 cgggcatttc cacggccgaa cagtgatccg ggacgtcgac cgcgtcttcg tcccgaatcg
      421 accggtcggg cgcgtcttgc gggaggagcc ccggcgagaa cgcacggtca cggtcggcgg
      481 agaacgacgc ctcgtttccc cgcgcgacga ccgcccgaat cgtggatttc gttttgagtc
      541 gacccgcgga tcaggacgag gaatgggagc cgcgcgagga ttcgagcgtc gggacgcccg
      601 gtaggatgtg gtcgggatgg tttccggctg atcctccgag acccggggaa tatcccagta
      661 gccgctctga tccgtggtgc taaacttagc gacgctctat ccgtttcgcc gacattttcg
      721 cggacttcag gaggtgcgtc gacatggatc agtcggaccg gcgtagcgcg ggtcgtatcc
      781 cgcatctagt cgaaattcgg tattcgagcg acagcccggt gttgtccgcc cgggtcacgg
      841 acgtgagcga gaagggcctg ttcgtggaag cccgaagccc attgccggaa ggctccatgg
      901 tctcgttcag ctttctcttg accaacacac cgtgggacaa accgatcacg ggccacggca
      961 aagtggtgtg gcggcaggag gcggtcggca tgggcatcga gttcaccgac ctctcggacg
     1021 agaatcgctc gaagctcaaa attttcgtag ggaatcaggc cgcgtaaggc cttcaccgcg
     1081 cgcccggaac aaccccctac ggtctgatcc ggcccaagag gcgcgacaag gccggggctc
     1141 cgaagagacg gaagccccgc aacgcctcgg gcggatgtcc ggccacgcac acgatcggca
     1201 cctgaccggc cacgcccatg atcaacggtt tgccggatcg catgggcact ccgttcacca
     1261 acacgccggg tgacccaacc gcggcgacca gatcccggac gaactcccga ccatccaccg
     1321 cggtgccgcc cgcgatgacg atcatcccgc agtggggcag cgcgtcgctc accgcgcgac
     1381 ggaacgtgtc gaagtcgtcg cggatcacgc cgtagcgttt tgggattccg ccctcccgtc
     1441 ggatcaacgc cgacagcgcg ggggtgttgc agtcccagat cgcgcccggg gtcagacgag
     1501 cggtgtgcgg gatgacctcg ttgcctgatc cgaagatccc cacgatcggc cgcgccgcga
     1561 ccggcacggt atcgatgccc tgggccgcga tcaacgacat ttcatccggg ccgatctgag
     1621 cgcccttggc caccaccacg gcgcctcgcg tgaggtcgca cccttgcgct tcgatattgg
     1681 cggcggcttg aaccggcgac gaacaggtga cgcgctgccc ggttcgtttc gcgtcccacg
     1741 ccctgatcac gccgcaggcg ccggccggca ccgcgctgcc ggtgaagact tcccacgcgg
     1801 tgccgggggc gatctgtttg ggtgcggcgt ctccgggtcg gatcgttccc gcgatcgtga
     1861 acgacaccgg gccggcgttt gacgccgccg tgtcgtcggc gttgaccaga aaaccctcga
     1921 caatcgcccg cgcgaagggc ggcgagtcga gggtggcggc gatcggctcg gccgcgacgc
     1981 ggtcgagcgc ctcgtcgagc gtgacgtgct cgacggcgga cgggccttcg ggaaacgcgt
     2041 tgagaaaccg cgcgagggct tcctggaccg gatcggattc ctgggatcgg ggcggtgatg
     2101 acacgagtca ggccggagag aattcccggc ctccgaccat acttcgacgg cgacgaatcg
     2161 cggccaagat ctcttgattg gtggcgtatt tgaactcttc gaccccggat ttcgcggctt
     2221 cctcgcgctc cacctgagcg aggagttgga gatcttcctt gttcaccaca tccggctgtc
     2281 gcgagtcgat gagggtcttg agccgtccgc agaactcgtc cagtttggaa gcgcaccgcg
     2341 tcggccgacc gtccagatac cggtgcacga cttccgcgca ccattcgccg tcgcgcttgg
     2401 cgatgcccac caagccttcg ctggccttgc gcgcccagcc cgcgacgaac acgccctcca
     2461 cgatcttgcc ggacgcctcg tcatacgctt ggaaccacgc gtcgtcgggt tcgttgcccg
     2521 acggggtcgg gttggtgacg aacatgccgc tcttataggg gagccccacg gcgtggtcca
     2581 cgcaatcgcc gaccgcgaag atcacgctgt cgcaggggat ttcatccgcg gtgccggtgc
     2641 ctttggcggc cgtgtcgtcg cccttctttt cgagtcgcgt gttctccact tccagggcgc
     2701 gcacgcggtt ctgcgcgtcg acgaggactt tgcgcggcga tgagagaaac cgaaacttca
     2761 tcatggttcc gctgaccgga ggatcgggct tcttgaactc gccgaccatg tcggcgagga
     2821 tctgatccgg gttctggccc acggcctcca ggcgctcgcg aattctcgcg aactcgcggg
     2881 acaaggcgtc cttgtcgatg ttcgcgcaca ccgcgtgcgt ttccttgggg ctgtattttc
     2941 gctccgccgg gccgcggcgc acgatggccg tgacctcctg cactttcttg tatcggatga
     3001 gccagtgggc gatgtcgatc atgacgtcgc cgataccgat cacgcacacg cgccgtccca
     3061 cgtcgaacgg ccgttcggag tacccgggga gttgattgta gtggaacacc acgtccttgg
     3121 cgtgaaacac gccggccgcg ttgtcgcctt ccaccccgat ggtcttggtg ccttgcgcgc
     3181 cggtggaaaa cacgatcgcg tcggcgccca gagcgcgaag ttcgtcgagc gtcagttcgc
     3241 ggtcccgccc cacgctggtg ttgccgaaat agagcacatg ggggttggaa aggatttcgc
     3301 ggtaggtttt ctggagaccg gtgcggagac ggtgtttgct ggggaagatc ccgtattcag
     3361 ccagccctcc gaccttgcag tcccggttca gaatcacgac gcgatgaccg tttttcgcga
     3421 attgcgcggc cgccgacatc cctgccggcc cggccccaac cacgacgatt ccataccctt
     3481 cttgtgggtg actcataaac aaactccact gatatcagga cgtgaacaac gagtgcccat
     3541 cgtagccgac ggttcggggg gaagtcaacg ggaaaggctg aaacgtgggc tggaagtccc
     3601 taaaaaatca agacttcctg ggtctttagg cctacgagcc tgttcagcga tgcgtcagat
     3661 gcaaggcgct gcgagcaccg gagcggagcg tacgaggtgg gtacgtgagc accggctgcg
     3721 cagcagcaac gcagcagatg accgtcgatg gacgggcgat cacgggccgg tgtagcggct
     3781 gacgccgccg tacgtcccga accacttggc gccggtcgaa tcaatggcca cggcgtagac
     3841 cgcgtcgtgg aacaggccgt cgaaggtggt gaatgtggtg aatttgcacg gaggcttgcg
     3901 gcctgatgag gccgcggtgc acgcggcggg gtccagccgg ctgacgcccg cgtcggtgcc
     3961 gatccacaac acgccgcgcc gatcgaacac cagggcgttg accacgttgc cggccaagcc
     4021 gtctcgggtc gtgtacgtga tccattgttt gccgtcaaaa cgcgacagcc cgccgcccca
     4081 cgtgccgaac cacttgtttt ggcctgcgtc gatcgcggtg gccaggacgt aattggggtt
     4141 atagtccgag atgtccttgt cgtattgctc gtggtgaggg ctcggggcct tgcccttggg
     4201 ttccccggac gcgcgcggga gttccgcgcc caggccgtcc ttgtgtgtcc aactggtcca
     4261 gcgtttcccg tcgtacatgg tcacgccgcc ctcggtgccg aaccagaatc gtttgtcgcg
     4321 gtccacggcc atcgagtaca cccacttgtc gaccaagccg tcgcccgtgc tgtaggtggt
     4381 gaacgtggtc ccgttgaacc ggctcgcgcc cttccacgtg gccgcccaca tggtgccgtc
     4441 tggcaggaac atggtctggt acacccacaa gtcgccgatg ccgtcgccgc gttggtagcg
     4501 ttggtagcgc ccgttgtccg cgggcgaacc ggcacacggc gtaaatcccc ccttgccccc
     4561 ctttttcaaa ggggggatgg aggaattggc agtgcagggg ttacggtaat cgccggtcgc
     4621 gcccgcgccg tagggcgtgt aggtcgtcca gcggttcgtg ccgtcgaatc gggtcaggcc
     4681 gccgccgtag gtgccgatcc acgccgcgtt gtcgggcgcg atggtgatcc ccagcaccac
     4741 gttgctgagc aggccgtcct tggtcgtgta caccttgtgt tgttcggtcg ggatgtggaa
     4801 cttcatcacc ccattggtgg tgccgatcca gatggaatcg ccctgcaccg ccaacgcgcg
     4861 caccaatgcg cccacgcgcc agctggtcca cagggtgtcg ggttgggctt gagcgggaag
     4921 gggaaaggtg atgctcgcgg ccagcgccgc gaccgcgatc gccagcccgg aatagttacg
     4981 gacgttcgtc gtgaaaccgc gcagacgcca gcgaaacggg cgcttggagc cacgaaagag
     5041 ggaggcgtac tctctgcggt acgctgaccg actgaggggc gagaccgccc gttgcagcag
     5101 cgtatccgcg gttggagcag aaggttcatg cagagtccgg gctataacca ggccttggct
     5161 aagaacacgc caagttgcac ggagataagc ccagcgaata cgctgagcgc catatagagc
     5221 gcggccgtga gccagcggcc ctccaggaac agaacgtggt tttcccatgc gaacgaggag
     5281 aacgtggtgt aggcgccgag aaacccgatc gccaacaggt actggacgtg ttcgggccgg
     5341 gcgaccagtc gctcgacgac tacggttccc acgatgccga tcaggaaaca cccggagaca
     5401 ttgaccacga acgtgccgag cggcacgcgt gcgccgaagg tcgatccgac ccacgagccc
     5461 acgatgaagc gcgcgttggc gcc
//
LOCUS       DBNGTT010000288         2060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2107629, whole genome shotgun sequence.
ACCESSION   DBNGTT010000288 DBNGTT010000000
VERSION     DBNGTT010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2107629"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="AC16BC_06460"
     CDS             complement(<1..929)
                     /locus_tag="AC16BC_06460"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HIR3241299.1"
                     /translation="MTSRRLLLVAVFGLAVGLPMSAAAAECPEWAAKIVSIEGRVEAL
                     RAGDATWQAVSLNDTYCPDERVRTLEKSRTAIQLQNETIIRLDQNTTVRFSGLDPQSP
                     TLLQLIRGRAFFLSRFPRTLTIETPFVNAASGGTEFHIEVNEEDQTTTITVIEGQMNV
                     TNEAGSTVVAAGQAAVAKAGQAPVLRVEIRARDAIQWALYYPPILSLRDLRLEATEGL
                     AETDWRTMVRRSIEAYQEGDLVKAFAALEGAPEDIADPGFYAYRASLLLSVGRVDQAR
                     TDIDKAQVLAPQDALALALQSIMAVVLNEPDKAL"
     gene            complement(926..1297)
                     /locus_tag="AC16BC_06465"
     CDS             complement(926..1297)
                     /locus_tag="AC16BC_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3241300.1"
                     /translation="MLPEKKRILLADDDPDVLLGIRDRLESFGFPVTAVANGREAVEA
                     MSGGAYALVIMDITMPEMDGIEALRMIRLRHPTTPVLIITSNIENAVAALVEGAGAYL
                     LKPIDHERLKTTVDRLVREDP"
     gene            complement(1301..>2060)
                     /locus_tag="AC16BC_06470"
     CDS             complement(1301..>2060)
                     /locus_tag="AC16BC_06470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3241301.1"
                     /translation="KYFDTRKLEAAHKQLQEVDRLKSMLLSHVSHELKTPLTSIKGFV
                     DNMLDGLTGELPGKQQDYLNRIRANTDRLSRMITNLLDQSRIESGTHRLDRVPLRLFD
                     LVVEVVEQFRPMAASKRMTLEMVCPDPTIRIWADPDKLIQVLSNLVDNAIKYTPPGGK
                     VTVATTRHDSERVMITVADTGEGIPAEAMGKLFEPFYQASRQPGTRAKGLGLGLSIVK
                     TLVELHGGNISVTSEVGKGSEFSILMPALTQKDA"
BASE COUNT          342 a          659 c          637 g          422 t
ORIGIN      
        1 agcgccttgt ccggttcgtt caggaccacg gccatgatgg attgcagcgc gagagccagg
       61 gcatcctgcg gagcgagaac ctgtgccttg tcgatatcgg ttcgggcttg gtccacccgg
      121 cccaccgaga gcaggagcga agcccgatag gcatagaatc cgggatcggc gatgtcttcg
      181 ggtgcgccct cgagcgcggc aaaagccttc acgaggtcgc cttcctggta cgcttcgatg
      241 gaccgccgca ccatcgtgcg ccaatcggtc tcggccaggc cttccgtggc ttccaaccga
      301 aggtcgcgaa ggctcaggat cggcgggtag tacaaggccc attggatcgc gtctctggcg
      361 cggatctcca cgcgaagcac cggggcttgg ccggccttgg cgacggctgc ttgcccagcc
      421 gcaaccaccg tgcttccggc ttcattggtc acgttcatct gcccttcgat caccgtgatg
      481 gtggtggttt gatcctcctc gttcacctcg atgtggaatt cggtgcctcc gctggccgcg
      541 ttcacgaacg gggtctcgat ggtcaatgtg cggggaaacc ggctcaggaa gaaggccctt
      601 cctcgtatga gctggagcaa ggtggggctt tggggatcca ggcccgagaa ccggaccgtg
      661 gtgttctgat caagacggat gatggtttcg ttctgcagtt gaatggcggt gcggctcttc
      721 tccagggttc ggacgcgctc atcggggcag taggtgtcgt tgagcgacac ggcctgccag
      781 gtcgcgtcgc ccgcgcgcag cgcttccacg cgcccttcga tcgacacgat cttggccgcc
      841 cactccgggc attcggcggc ggccgcgctc atcggcaacc cgacggccag cccaaacacc
      901 gcgaccagaa gcagccttcg gctcgtcatg ggtcctcccg gaccaagcgg tccaccgtgg
      961 ttttcaggcg ttcgtggtcg atcggcttga ggaggtacgc gccggctccc tcgaccaacg
     1021 cggcgacggc attctcgata ttggaggtaa tgatgagaac cggcgttgtc ggatgtcgca
     1081 gccggatcat ccgaagcgcc tcgatcccgt ccatctccgg catcgtgatg tccatgatca
     1141 ccagcgcgta cgctcccccg ctcatcgcct ccaccgcctc ccgtccgttc gcgaccgccg
     1201 tgaccggaaa tccgaacgac tccagacggt cccggatgcc cagcaagaca tccggatcat
     1261 cgtccgcgag cagaatccgc tttttttccg gcagcactgg ctaggcgtct ttctgcgtga
     1321 gcgccggcat gaggatgctg aactcgctcc ctttcccgac ctcgctcgtg accgagatgt
     1381 tgccgccatg gagctccacc agcgtcttca cgatggagag ccccaacccc aggcctttgg
     1441 cgcgggttcc gggctggcgg cttgcctggt aaaacggctc gaacagtttc cccatggcct
     1501 cggccggaat accttctccg gtatccgcca cggtaatcat cacgcgctcg gagtcatggc
     1561 gggtcgtcgc caccgtgacc tttcctcccg gcggcgtgta tttgatggcg ttgtccacca
     1621 gattgctgag cacctggatc aatttgtcgg ggtcggccca gatccgaatc gtgggatcgg
     1681 gacagaccat ttccaaggtc atccgcttgg aggcggccat gggacggaat tgctccacga
     1741 cctccaccac cagatcaaag agacgcagcg gcacccggtc gagacggtgg gtcccggact
     1801 cgatccgcga ctggtccagg aggttcgtga tcatgcggct gaggcggtcc gtgttggccc
     1861 gaatccgatt caggtagtct tgctgtttcc ctggaagttc cccggtcagt ccgtccagca
     1921 tgttgtcgac gaaccccttg atcgacgtga gcggggtttt cagctcgtgc gacacatggg
     1981 agagcagcat ggacttgagc cggtccactt cctgcaactg cttgtgggcg gcctcgagtt
     2041 tcctggtgtc aaaatacttc
//
LOCUS       DBNGTT010000289         2983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1212485, whole genome shotgun sequence.
ACCESSION   DBNGTT010000289 DBNGTT010000000
VERSION     DBNGTT010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1212485"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="AC16BC_06475"
     CDS             complement(<1..755)
                     /locus_tag="AC16BC_06475"
                     /inference="COORDINATES: protein motif:HMM:NF021499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3241302.1"
                     /translation="MLKQRDSRFGLRFKFVVITSLLLLCVSTVLTVVSLARTRQYLED
                     ALIKRGVSLAKNLAHNSTYGISIGDSSSLVSYIKGVMNEVDVSSVKILDGRGIVWVHS
                     DPTYNKDVDNDPLSAKAVQATEGFVEWTEKNGQKYVAVIEPVLFEAGTQGPMGTGEKS
                     RIGTVVMALSPNSLESQLQSIIWWSVLLTLLTVAAGIGVALYFVKTNMAPLERMAAVV
                     TRVADGDFTQTIEVTSRDEIGVLASGFNRMASSL"
     gene            complement(783..1289)
                     /locus_tag="AC16BC_06480"
     CDS             complement(783..1289)
                     /locus_tag="AC16BC_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR3241303.1"
                     /translation="MAAPATSPALDSLPAESGRRVEEFLGFRLDNEEYAVRIRSVREI
                     IRPTEITPIPRSPADILGIISLRGTIVPIFDLRRRLGLPRRESDHKSRIVVIVIDGAP
                     VGMVVDQVTEVIMADPEGLEPPPTTMGEQEASFVTATLRHRERLLGVLHLERLVAIEP
                     VTPLRAVA"
     gene            complement(1523..1753)
                     /locus_tag="AC16BC_06485"
     CDS             complement(1523..1753)
                     /locus_tag="AC16BC_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3241304.1"
                     /translation="MSYGPNEVCRRVGLSPRQLEYWVLIGVVTPVTERHGTKLFRRFT
                     DHELRVLKRVKALTDEGFLVSRAAEKVRQEAA"
     gene            complement(2041..>2983)
                     /locus_tag="AC16BC_06490"
     CDS             complement(2041..>2983)
                     /locus_tag="AC16BC_06490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HIR3241305.1"
                     /translation="ARDLGKEIQVTTSGEETKLDKSMVEAIADPLLHLIRNALDHGIE
                     SREDRDRAGKPAIGTIHLNAAQKGHTVTITVADDGAGINLAKVYEHALMKKLAIPDKE
                     YTNREIIDFLFQPGFSTADTITEISGRGVGLDVVARHIAKLNGVVEVNTELGQGTGFI
                     LTLPITLVIIKALIVGVGNQTYAIPISSVSESVMIDHTQVVTVGGRPVIHLRDHTLAL
                     LRLSDLFGVPPARDTDGRLYIIVVGLAEKRLGLVVDTIFGQQEIVIKSVGSLLQGIPG
                     IAGAAELGNRKTILVLDVGALIEEAVSRRATALPLAA"
BASE COUNT          570 a          993 c          855 g          565 t
ORIGIN      
        1 aggctcgacg ccatccgatt gaatccgctc gccaacacgc cgatttcgtc gcgcgaggtc
       61 acctcgatgg tctgggtgaa gtctccgtcg gccacccggg taacgacggc cgccatccgc
      121 tcgagcggcg ccatgttcgt cttcacgaaa tacaacgcca ccccgatgcc cgcggcgacc
      181 gtcaggagcg tcagcaacac gctccaccag atgatgctct ggagttgcga ctcgagcgaa
      241 ttgggggaca gagccatcac cacggttccg atgcgcgatt tttctccggt gcccatcggc
      301 ccctgcgtcc ccgcctcgaa gaggacgggc tctatcacgg cgacatactt ctgtccgttt
      361 ttctcggtcc actccacgaa tccctcggtc gcctgaaccg ctttggccga cagaggatcg
      421 ttgtcgacat ctttgttgta agtcggatcg ctgtggaccc acacgattcc acggccatcg
      481 aggattttga cggaggacac gtcgacctcg ttcatgaccc ccttgatgta gctcaccagg
      541 ctggatgaat cgccgatcga aatcccgtac gtactgttgt gggccaggtt cttggccaac
      601 gatacgccgc gcttgatcaa cgcgtcttcc aagtactggc gggtcctggc gagcgagacc
      661 acggtcagca ccgtcgagac gcacaacagc agcaggctcg tgatgacgac aaatttgaac
      721 cgcagtccga accgagaatc acgctgtttc agcatacgct cccccttcac agacgcatca
      781 cgttaagcca ccgcccgaag cggcgtcacg ggctcgatcg ccacaagccg ttcaaggtgc
      841 aacaccccga gcagccgttc tcgatgacgc aacgtcgctg tcacaaacga tgcttcctgc
      901 tcccccatgg tcgttggcgg cggctccaac ccctcgggat cggccataat gacttccgtg
      961 acctgatcga ccaccatccc caccggcgcc ccgtcgatca cgatgaccac gatgcgcgat
     1021 ttgtgatcgg actcgcgccg cggcaatccg agccgccgtc gaagatcgaa gatgggcacg
     1081 atggtccccc gcaacgaaat gatccccaga atgtcggccg ggctcctcgg aatcggcgtg
     1141 atctcggtcg gtcggataat ctcccggacc gaccggatcc ggacggcata ttcctcattg
     1201 tccaaccgaa agcccaggaa ctcttcgaca cgccggcccg actccgccgg caaggaatcg
     1261 agcgcggggg aagtcgccgg cgcagccacc gcgggcgccg gcgatgccgg acgctcctcg
     1321 acccccgtct ccgcccgagg gcgttcttcg gttggggcca ccggaggtag caccgcaccg
     1381 ttggagctaa ccgattcgac cggcgtatcg cccgcagccg gctctgcggt tccgccgggg
     1441 gctcgtacgg ctttagctcg ggcggtaccc gcctttggcc ccgcccctcg tcgcggtttg
     1501 gacacttgtt tgccggatcg cttcacgccg cctcctgccg gaccttctcc gccgcccgac
     1561 tgaccaagaa gccttcgtcc gtcaacgcct tcacccgttt gagcacgcgc aactcgtgat
     1621 cggtgaaccg gcggaacagc tttgtcccgt ggcgctcggt caccggcgtg accacgccga
     1681 tcaacaccca atactccagt tgccgcggcg agaggccgac tcgtctgcac acttcgttag
     1741 gaccgtacga catagttcgc atcctggatt ccctcacatc ccacaggaat gtgggacagc
     1801 gtggcgtgag acagcaagtc ctatgccgac aagaaatgat ccgagcgaac cgaaaatgac
     1861 cactccttga cgttggccga gagctacccc gtcgctggta ggtaaccgag gaatcggaat
     1921 gtgtcaaaat ttggcgctga aacgggtgaa acgctgccaa gccattcaac tgcgacgtac
     1981 gcgcggatcg agtgtccacg cggaagatcc cacgtgggaa ccggggacgt gaatgtgcgg
     2041 ctacgccgcg agagggaggg cggtcgcgcg ccgactgacc gcttcttcga tcagggcgcc
     2101 tacatcgagg acgaggatgg tcttccggtt gccgagctcc gcggctcccg cgatcccagg
     2161 gatgccctgc aacaacgagc cgacggactt gatcacgatt tcctgttgcc cgaagatcgt
     2221 gtcgacgacg agtcccaaac gcttctccgc caaacccacc acgatgatat acaatcggcc
     2281 atccgtgtcc cgagccggcg gcaccccgaa cagatcgctc aaccgcaaga gcgcgagcgt
     2341 gtggtcgcgg agatgaatca ccggtcgccc gccgaccgtg acgacctgcg tgtggtcgat
     2401 catcacgctc tccgacacgg agctgatggg aatcgcgtag gtttggtttc cgaccccaac
     2461 aatgagcgcc ttgatgatca ccaacgtgat cggcagcgtc aggatgaaac ccgttccctg
     2521 cccgagttcg gtattcactt cgacgacgcc gttgagcttc gcgatatgcc gagccaccac
     2581 gtcaagcccc acgccgcgtc cggagatctc ggtgatcgtg tccgctgtgg agaatcccgg
     2641 ttggaacagg aagtcgatga tctcgcgatt cgtgtactcc ttgtcgggaa tcgcgagttt
     2701 cttcatcaac gcgtgctcat agaccttggc gaggttgatg ccggcgccgt cgtccgccac
     2761 cgtaatcgtc acggtatgcc ccttctgcgc cgcgttgagg tggatagtac caatcgcggg
     2821 tttgccggcc cggtcccggt cctcccgcga ctcgatcccg tgatccaagg cgttgcggat
     2881 caaatgcagg agcgggtcag cgatcgcctc gaccatcgat ttatcaagtt tggtttcttc
     2941 gccagaggtc gtgacctgaa tttccttccc gagatcgcgc gcg
//
LOCUS       DBNGTT010000290         4689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_190838, whole genome shotgun sequence.
ACCESSION   DBNGTT010000290 DBNGTT010000000
VERSION     DBNGTT010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_190838"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3055
                     /locus_tag="AC16BC_06495"
     CDS             <1..3055
                     /locus_tag="AC16BC_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3241306.1"
                     /translation="VGSAAGAATAAIDGGLVDGTTYYYVVRAYDGTFESPNASEASAA
                     PRSNAVEFLHQETSAVDSLYRRLKQDAPGTNGDPVSISASYQTINLANEVPGSFFIEA
                     FETQAGDPGGAVTIPAGSVFRVTLYMDKTTDAAGAGVIYPRASVSKVDASAVRTTVCV
                     ADDATNPGLTPLTATTTALTFNCLNAAPVTLAVTDRLWLWTGVRLTTSPTINVSARLF
                     VEGTTGSPPAYQSQLTIPWHETTAPTAPNGLIAADHPNDDGGAVDLSWTGVADPDVAG
                     YVLYRSTTSGGPYTRVNAYPSLVASYVDRSVSAGTFYYYVVRSYDTSGNESGNSNQAS
                     VTPLDTLPPPAVIDFAAGDGADSGATLSWTNPVDPTLGEVLVARKTGGYPTGHADPLA
                     AVVYDSTTPVSGGSVSFFDTGLTNGTTYYYAVYTRDAAGNWNDATTPAGNADTAVPSS
                     LAPASPTNVTASDRPADQGGAVVLSWTPSTAPGIVEQRVFRSTTSGGPYTLVVALAGN
                     SATSYTDVGLVNGVMYFYVVRAWNGTQESGDSNQAAAVPLDNVAAAAPSALTVVDTPA
                     DSGTALNLTWTVSVSSDVTQQRVYRGTVSSGPYSLVAVIADNTTSAYTNTGLTTGTSY
                     YYVVRAFDGTQESAPSNEADAVPIDDRAPTNVTAVDAPADEGGSIDLTWTPSSMPGLT
                     EQRVYRSTTTGGPYDLISTVPDNTTASYADSGLTNGTTYYYVLRSFDGTEESANSAEA
                     SAAPLDNLAPAPPTGLAAADRPADQGGALVMTWSPSTAADVTQQRVYRSATSGGPYTL
                     VLAISDNTTASYTDTGLTNGTPYYYVVTAFDGTQESPNSTQVSAVPLSNATFHMHNEP
                     SALSGARQLLQAGPDAAPTAIQSGQLVGGVNAIADFETQLGDPNGATTLAAGSTVSFT
                     LYLQETETSGTMMPYAAVYQNTAAAGNLICSGTGGTSITTTMTAYTFTCAVPAGGVSF
                     VPTDRLYMAVGVNATSVPTATNRVNVGVEGTLNGARDSRVVVPWP"
     gene            3046..>4689
                     /locus_tag="AC16BC_06500"
     CDS             3046..>4689
                     /locus_tag="AC16BC_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241307.1"
                     /translation="MAVGELTDRMLLGTLRLGRRRGPGLSRWRTGAIGLVVCVMALTG
                     CNSGGGGGGDGPIPTVIIIPPGDVAKGQSVQLFAMVVDAGPGVVWSMQSGAAYGTVTP
                     NGVFTAPLTVPDGPAIVRATSRDDSRGTAVASINVVVGWTTSGPGAVTVNPNQALRPT
                     GPAGTANTFSGGQRSVAVSGSTVYAVWNEDVTGDDDIYLAVSRDRGATFGAPIRVNYD
                     DITTAPQLAPSVAVDASGRAVIAWIDARNDPDPAPNNHGYDIYVAEATISGSGTVTLG
                     PNQRVTTHSNGPACDYDDQNVPCDPSVALAVSLGGDVYMAWTDASSGFDMDVLLTKGT
                     RLVSEAFQFAFAPATPVHAYLTSDQSRPSVAVDSAGFVLVAWNDPRSGDQDIYWSRGQ
                     FAANGAVTWTAQDVMVNLQTADDQVSPSVAFDQDGVPYVAWSQQVGASERRKLYFARG
                     AKSNLVVAGNVDVLPTVDADQNFPSLSVIGTDATIAFADNRGCLPPACPFDPVNPKGT
                     GPTDVYLVRSVDGGATFGDSLRVNDDPAGTALHGRASLAV"
BASE COUNT          830 a         1478 c         1572 g          809 t
ORIGIN      
        1 ggtggggagt gcggcgggcg cggccactgc ggctatcgac ggaggcctcg tcgacggcac
       61 gacgtattac tacgtggtgc gcgcgtatga cggcacgttt gagagcccga acgcctccga
      121 agcgtccgcc gcgcccagat ccaacgcggt ggagttcctc catcaggaga cgtcggcggt
      181 tgactccctg taccgccgct tgaaacagga cgcgccgggg accaacggcg acccagtctc
      241 gatcagcgcg tcctaccaga cgatcaatct ggccaacgaa gttcccggct cgttcttcat
      301 cgaggcgttc gaaacgcagg ccggtgatcc gggcggcgcc gtgacaattc cggccgggtc
      361 ggtcttccgc gtgaccctgt acatggacaa gaccacggac gcggccggcg ccggcgtcat
      421 ctacccgcgc gcgtcagtgt caaaagtcga cgcgtccgcg gtccgaacca cggtctgcgt
      481 ggccgatgac gccaccaatc cggggctgac gccgttgacc gccaccacga ccgcgctgac
      541 cttcaactgt ctcaatgccg cgccggtcac gctggccgtg accgatcgcc tgtggctgtg
      601 gaccggtgtg cgtctgacca cgtcccccac gatcaacgtc agcgcccggc tgttcgtgga
      661 gggcacgacc ggctcgcccc ctgcgtatca atcgcaattg acgatcccgt ggcacgagac
      721 cacggcgccg acggcgccga acggcctgat cgcggcagac catcccaatg acgatggcgg
      781 cgcggtggac ctgtcgtgga ccggcgtggc ggaccccgac gtggcgggct atgtgctcta
      841 tcggtcgacc accagcgggg gtccgtatac gagggtcaat gcctacccga gcctcgtcgc
      901 gtcctacgtc gaccgcagcg tgtcggctgg gaccttctat tactacgtgg tgcggtcgta
      961 cgacacgagc gggaatgaga gcgggaattc aaaccaagcg tcggtcaccc cgctcgatac
     1021 gctgccgccg ccggcggtga tcgattttgc cgcgggagac ggcgcggaca gcggcgcgac
     1081 gttgagctgg accaatccgg ttgacccgac gctgggcgag gtgctcgtgg cgcgcaagac
     1141 cggcgggtat ccgaccggcc acgccgatcc cctggccgcg gtggtttatg attccacgac
     1201 gccagtctcc ggcggatcgg tctccttctt cgacaccgga ttgaccaacg gcaccacgta
     1261 ctattacgcg gtgtatacgc gggacgcggc gggtaactgg aacgacgcga ccaccccggc
     1321 cggcaacgcg gacacggccg tgccgtcgag cctggcgccg gcgtcgccga ccaacgtcac
     1381 cgcgtccgac cgcccggctg atcagggcgg agccgtcgtc ctgtcgtgga ccccctcgac
     1441 ggcgcccggc atcgtcgagc agcgggtgtt ccgcagcacc acgagcggcg ggccgtacac
     1501 attggtcgtg gcgttggccg gcaacagcgc cacgtcgtac accgacgtcg gtctggtcaa
     1561 cggcgtcatg tacttctatg tggtgcgggc ctggaacggg acccaggagt cgggagactc
     1621 caatcaagcc gcggcggttc cgctggataa tgtggctgct gcagcgccct cggccttgac
     1681 ggtcgtggat acaccggctg attcggggac cgcgctgaat ctgacgtgga ccgtttccgt
     1741 cagcagcgac gtgacgcagc agcgcgtgta ccgtggtacg gtcagcagcg ggccctacag
     1801 cttggttgcg gtgatcgccg ataacacgac ttcggcgtat acgaatacgg ggctgacgac
     1861 cggcacaagc tattactacg tggtgcgcgc gttcgacggc acgcaagaaa gcgccccgtc
     1921 caacgaggcg gacgcggtgc cgatcgacga tcgtgcgccg acgaacgtga ccgcggtcga
     1981 tgccccggct gacgaggggg gatcgatcga cctgacgtgg accccgtcga gcatgcccgg
     2041 gttgacggaa caacgggtgt atcgctcgac aacgacgggc ggaccatatg acttgatttc
     2101 cacggtcccg gacaacacga cggcgagtta cgcggattcc ggcttgacca atgggacgac
     2161 atattactac gtactccgct cgttcgacgg gaccgaggag tccgccaatt cggcggaagc
     2221 gtcggctgcg ccgctcgaca atcttgctcc ggccccgccg accggcctgg ctgcggcgga
     2281 tcgtccggcc gaccaaggcg gcgcgctggt gatgacctgg tctccgtcga cggctgcgga
     2341 cgtgacgcaa caacgggtct accggagcgc gacgagcggc gggccgtaca cacttgtttt
     2401 ggcgatctcg gacaacacga ccgcgagcta tacggatacg ggattgacca acggcacgcc
     2461 gtattactac gtggtgacgg cgttcgacgg cacgcaggag tcgcccaact ccacgcaagt
     2521 cagtgccgta cccctgagta atgccacgtt ccacatgcac aacgaaccgt ccgcgttgag
     2581 cggagctcgt cagttactcc aagccgggcc cgacgcggcg cccacggcca ttcagagcgg
     2641 tcagctcgtg ggtggcgtga acgccattgc ggatttcgag acgcaacttg gcgatccgaa
     2701 tggcgccacg acgctggccg cggggtcaac ggtgtccttc accctgtacc tgcaggagac
     2761 cgaaacttcc gggaccatga tgccatatgc tgcggtttat cagaacacgg ccgcggcagg
     2821 caacctgatt tgcagtggga cgggcggcac gtcgattaca acaaccatga cggcctacac
     2881 gttcacatgt gcggtcccgg ccggcggcgt gagctttgtg ccgacggacc gactatatat
     2941 ggcggttggg gtgaacgcta cctcggttcc gacggcgacc aaccgcgtga atgtgggggt
     3001 cgaaggtacc ctcaacgggg cccgcgattc cagggtggtg gtgccatggc cgtaggcgaa
     3061 ttgaccgatc gaatgctcct cggtaccctt cggctcggtc ggcgccgagg accgggcctg
     3121 agccgatggc gcaccggggc aatcggtctg gtggtatgtg tgatggcgct gaccggctgt
     3181 aattcgggcg gcgggggcgg cggtgatggg ccgatcccca cggtgatcat tattcccccc
     3241 ggcgacgtgg ccaaggggca gtcggtccag ttgttcgcca tggtggtgga cgctggccct
     3301 ggtgtggtgt ggagcatgca aagtggagcg gcatacggca cggtgacgcc caacggcgtg
     3361 ttcaccgcgc cgctcaccgt gcctgatggc cccgcgattg tcagggccac cagtcgggac
     3421 gactcgagag ggacggcggt tgcgagcatt aatgtggtcg ttggctggac gaccagcgga
     3481 cccggggccg tgaccgtgaa tccgaatcag gccctcaggc caacgggccc cgctggaaca
     3541 gccaatacgt tctccggagg acaacggagc gttgccgttt cagggtcgac cgtctatgcg
     3601 gtctggaacg aggatgtgac cggggacgac gatatctatt tggcggtgag ccgagaccgc
     3661 ggcgcgacat tcggcgctcc gattcgggtc aactatgatg acatcacgac cgcgccgcaa
     3721 ctcgcgccat ccgtggccgt cgacgcctcg ggccgtgcgg tgatcgcatg gatcgacgcg
     3781 cggaatgatc cggatccggc gcccaataat cacggttatg acatctacgt ggccgaggcg
     3841 actatcagcg ggagtggaac cgtcacactc ggtccaaatc agcgtgtgac gacacattca
     3901 aatggccctg cgtgtgacta cgacgatcaa aacgtcccat gcgatccctc cgtggcactg
     3961 gccgtaagtt tggggggaga cgtgtacatg gcgtggacgg atgcgtcgag tggatttgac
     4021 atggacgtcc tgttgaccaa aggcacgcgc cttgtatcgg aggcgttcca gtttgcgttt
     4081 gcgccggcaa ccccggttca cgcgtatctc acgtcggacc aatcgcggcc gtcagtcgcc
     4141 gtcgacagtg cgggatttgt cctggtggcg tggaacgatc cacggtcggg agatcaagat
     4201 atttattgga gccgaggaca gttcgcggcc aacggggcgg tcacttggac ggcccaggac
     4261 gtgatggtca accttcagac ggccgacgat caggtctctc ccagcgtggc gtttgatcag
     4321 gacggcgttc cctatgtcgc ctggagtcag caggtaggcg ccagtgaacg acggaaactc
     4381 tacttcgctc gtggcgccaa gtcgaactta gtcgttgccg gcaacgtcga cgtgctcccg
     4441 acggtggatg cggaccagaa cttcccgagc ttgtcggtga tcgggaccga cgcgaccatc
     4501 gcgttcgcgg ataaccgagg atgccttccg ccggcctgtc cattcgatcc ggtgaatccc
     4561 aaagggaccg ggcccacgga tgtctatctc gtgcgcagcg tggacggcgg ggccacgttc
     4621 ggggacagtc tgcgcgtgaa cgatgacccc gcgggcacgg ccttgcacgg ccgcgcttcc
     4681 cttgccgtc
//
LOCUS       DBNGTT010000291         3224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_957487, whole genome shotgun sequence.
ACCESSION   DBNGTT010000291 DBNGTT010000000
VERSION     DBNGTT010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_957487"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1314)
                     /locus_tag="AC16BC_06505"
     CDS             complement(295..1314)
                     /locus_tag="AC16BC_06505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3241308.1"
                     /translation="MDERVTSARNGRGSGRAAWFVVLAALVSIGFPAVGREFWVAELS
                     AHFVVPYAVASLALAAWFTRANRPVLAACALAICAVQVARVVPLWAQPDVQAGVGAPE
                     RLTIAQINVYYRNTEPSRVVDWVLARDPDVAVLVEVTPAWSAPMARLRARYPHSLVRI
                     PPDGSGVAVFARSADPSLRVEPLGDAWCPAIVLDVPQPTGPPVLALFATHLRSPITPR
                     DAWARNRQLTALGRRVADEPALNKVVVGDLNVTRWSPWFADLIRVAGVRDGQEGFGLG
                     GSWPSRFGRWWGIPIDHTLVSPGVRVVQRVVGPDLGSDHLPVTTTIEFSPGAGVGVDD
                     RDFSA"
     gene            complement(1307..2200)
                     /locus_tag="AC16BC_06510"
     CDS             complement(1307..2200)
                     /locus_tag="AC16BC_06510"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3241309.1"
                     /translation="MHTVAFLGLGIMGSRMATRLAGAGFVVRAWNRRRSVAEALRVGG
                     VTVVDSPAEAALGCDVAITMLGDPASVRDVALGPGGLIDALAPGAVYVDMTTVDPDTS
                     RGLSCACAARGVAFVDAPVTGSKLAAAKGELVLMVGGAADVIARLAPVFAPLARHVVR
                     MGPVGSGSMMKLANNLAIAGSILAVCEAMTLGRQAGLDDAAMLDVLTHSALASPLLAL
                     KGDALRRRDFEPHFAFKHMAKDIRLACAEAQALGAPHPLGDLLDALFAAGLDQELGEE
                     DFAALVKVVETLGDEPGRRRG"
     gene            2296..>3224
                     /gene="add"
                     /locus_tag="AC16BC_06515"
     CDS             2296..>3224
                     /gene="add"
                     /locus_tag="AC16BC_06515"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR3241310.1"
                     /translation="MTHSYTQDTVNGPGPDLSQAVRRIPKIELHQHVDGSIPIPITWE
                     LMTKHGLSPVDTVDDMERLLTLQPEEEGTLIRYLDKFHYPAWITQFYGNITRVVEAIV
                     AEATTHGVTALELRYSPIIHTYAGLTFRQAISAVLSGLNHAEDHYGVRTGLIIIAMRH
                     QGPHIAKILARQAISEAQHLHSRTGVIGFDLAGAERGNPPRLFKEAYDIASKGGLGLT
                     VHAGEDEGPNCIWQALDVLGVTRIGHGCSAVQDPALVKRLAADRVMVECCLTSNYHTG
                     AVKRGQPHPIFTFLEAGVPVSVCTDNTTVSRTN"
BASE COUNT          591 a         1184 c         1016 g          433 t
ORIGIN      
        1 cggcgtcgca ctcccggtac gcgcgatcga ccgactcgac gaacccgcgc caggaatcgg
       61 ggaccccggc agggccgccg aaccagcggg cgaggtcgcg cgcgagccga tcgtggaagg
      121 tgggcaccta ggttgccgaa gacaaagtga cggtcatgtt cggtcgtcga tcgtgtccga
      181 cgtgacgaga cgcgcgcgat gacgagtggc tccgagtata acaacggagc cgagcgggtc
      241 aagcccgctg gccgcggaga cgcctcatcg gacggccgat cgcgatggga tcggttatgc
      301 gctgaagtcg cggtcgtcca cacccacccc cgctcccgga gagaactcga tggtcgtggt
      361 caccggcagg tgatcggacc ccaggtcggg gcccaccacg cgttgcacga cgcgcacgcc
      421 gggcgacacc agggtatggt cgatcggaat tccccaccac cgtccgaatc gactgggcca
      481 actcccgccc aatccgaatc cttcctgacc gtcccgcacg cccgcgaccc gaatcaggtc
      541 ggcaaaccac ggcgaccagc gggtcacgtt cagatcaccg accacgacct tattgagggc
      601 gggttcgtcg gcgacccgcc gaccgagggc cgtcaactgc cggttgcgcg cccaggcatc
      661 cctcggggtg atcggactgc gcaagtgggt cgcaaacagc gccagcacgg gcgggccggt
      721 cggttgcggc acgtccagca cgatcgcggg acaccaggcg tcgcccaggg gttcaacccg
      781 caaggacgga tccgcgctcc gcgcgaagac cgcgacgcct gatccgtccg gcggaattcg
      841 aacgagcgag tgggggtacc gcgcgcgcaa ccgcgccatg ggcgccgacc aggcgggggt
      901 gacctcgacc agcaccgcca cgtccgggtc ccgcgcaagg acccagtcca ccacgcggct
      961 gggctcggtg ttccgatagt agacgttgat ctgcgcgata gtcaggcgct cgggcgcgcc
     1021 gactccagcc tggacatccg gctgcgccca gagcggaacc acccgcgcga cctgcaccgc
     1081 gcagatcgcc agcgcacatg ccgcgagcac cggccggttc gcgcgcgtga accacgccgc
     1141 cagggccagc gacgccacgg cgtatggcac cacgaaatgg gcgctcaact ccgcgaccca
     1201 gaattcccgt ccgaccgccg gaaacccgat cgagaccagg gccgccagca cgacgaacca
     1261 cgccgcgcgc ccggaacctc gcccgttgcg cgcgctcgtc acccgctcat ccacgacgcc
     1321 gtcccggttc gtcgccgagt gtttccacga ccttcaccag cgcggcaaag tcttcttcac
     1381 ccagctcctg atccagtccc gcggcaaaca gcgcatcgag aagatccccc agcgggtgcg
     1441 gcgcgccgag ggcctgggct tcggcgcaag ccaaacggat gtccttggcc atgtgtttga
     1501 acgcaaaatg cggctcgaag tcgcgtcgcc tgagcgcgtc gcccttgagc gccagcaacg
     1561 gcgaggccag cgcgctgtgg gtcaacacgt cgagcatggc cgcatcgtcc agcccggctt
     1621 gccgccccaa cgtcatcgcc tcgcacacgg ccaggatcga gcccgcgatc gcgaggttgt
     1681 tggcgagctt catcatcgaa ccggagccca ccggccccat ccgcaccacg tgtcgggcca
     1741 gcggcgcgaa gacgggcgcg agccgggcga tcacgtcggc cgcgcccccg accatgagca
     1801 ccagttcgcc ctttgccgcg gccagtttgc tgcccgtaac cggcgcgtcc acgaacgcga
     1861 cgccgcgcgc ggcgcacgcg cacgagagcc cgcgcgacgt gtcgggatcg accgtggtca
     1921 tgtccacgta cacggcgccc ggggccagcg cgtcgatcag gccgccgggg ccgagcgcca
     1981 cgtcccgcac cgacgccgga tcgcccagca tggtgatcgc gacgtcgcac ccgagggccg
     2041 cttccgcggg cgagtccacg accgtgaccc cgccgacacg aagggcctcc gcgaccgatc
     2101 gccgacggtt ccacgcccgg acgacaaaac cggcgcccgc aagacgcgtc gccatccggc
     2161 tccccatgat tcccaatccc aaaaacgcga ccgtgtgcac ctcactcgcc tccgctcgac
     2221 gtccagcata acgatacgaa tccagccctg caagggcgca gcggtcgtcc cgacgttcca
     2281 cgctttgaca tgcgtgtgac ccactcctat actcaggaca ccgtgaacgg cccgggtccc
     2341 gacctctcgc aagcagtccg tcgtatcccg aaaatcgaac tccaccagca cgtggacggc
     2401 tccattccga ttcccatcac ctgggagctg atgaccaaac atggtctctc ccccgtggac
     2461 acggtggacg acatggagcg gctcctgacc ctgcagcccg aagaggaagg cacgctgatc
     2521 cggtatctgg acaagttcca ctacccggcc tggatcaccc aattttacgg gaacatcacg
     2581 cgcgtggtcg aagcgatcgt ggccgaggcc acgacgcacg gcgtcacggc gctggagctc
     2641 cggtactcgc ccatcatcca cacctacgcg ggattgacct tccgccaggc gatcagcgcg
     2701 gtgttgtcgg gcctcaacca cgccgaagac cattacggcg tgcggaccgg actgatcatc
     2761 atcgccatgc ggcatcaggg cccgcacatc gcgaagatcc tcgctcgcca ggccatcagc
     2821 gaagcccagc acctgcacag ccggaccggc gtgatcggat tcgacctggc gggcgcggaa
     2881 cgcggcaatc cgccccgcct cttcaaggag gcgtacgaca tcgcgagcaa gggcggattg
     2941 ggactgaccg tccacgccgg agaagacgaa ggaccgaact gcatctggca agcgctcgac
     3001 gtgctgggcg tgacccggat cgggcacggg tgttccgcgg tgcaggaccc ggcgctggtc
     3061 aagcggttgg ccgcggatcg cgtgatggtc gagtgctgtc tgacctccaa ctatcacacc
     3121 ggcgcggtca aacgcgggca gccgcacccc atcttcacgt ttctcgaagc cggcgtgccc
     3181 gtgtcggtgt gcaccgacaa caccacggtc tcgcgcacga acca
//
LOCUS       DBNGTT010000292         4580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1596082, whole genome shotgun sequence.
ACCESSION   DBNGTT010000292 DBNGTT010000000
VERSION     DBNGTT010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1596082"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="AC16BC_06520"
     CDS             <1..976
                     /locus_tag="AC16BC_06520"
                     /inference="COORDINATES: protein motif:HMM:NF022007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HIR3241311.1"
                     /translation="LLKDMRVKDLVFEGGLTRESELGQAELIRLENGEETGQVRCAVV
                     PIDLAKAMADDPTHNVPLRKDDELLVKQVGELKREYHVAVEGQVRFPGSYAIRAGERI
                     SSLIRRAGGLTDKAFLRGAVFTRESVRIEQQNRLEDLTASYTQRVTAEAAAVATSGLS
                     KEETETARFELSARQNSIKLLTQDTALGRVVVHLRDPDALEGTPEDLVLEDGDKLVIP
                     QVPSSVTVLGTVRNPTAIAAHEGGSVKEYIRLAGGYLPEADRKAVYVVKADGSSVGSD
                     QVKTIEAGDSILVPPQMDPKYRPLPLWRDVATIVGQFAIAIAALTVIF"
     gene            976..1734
                     /locus_tag="AC16BC_06525"
     CDS             976..1734
                     /locus_tag="AC16BC_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR3241312.1"
                     /translation="MTRTRELMVDASEPAADQRPEDEPTILDYARVLWGGRWLILVIT
                     LLVTAAAVGISLTMSKSYTAKSTLMPLGLDRGGGLGALAGALSIENPGMKLMAVLQSR
                     TVAEDVVGKLNLEPEFAVGRDRAPTHAEVIESFQKRVLKVTASQGMIVVKAAWRDPEL
                     SAKIANTAVLSAARYLNEHSISTSFQILDEAVAPAKPSSPKIALNTAAAFVLSGFAAV
                     CLVFIRQYLREVRRAESARAAAVAPSGGASSGPA"
     gene            1755..3161
                     /locus_tag="AC16BC_06530"
     CDS             1755..3161
                     /locus_tag="AC16BC_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HIR3241313.1"
                     /translation="MLAACCASGALAGEPHLTVGDPVYERIDRLAALGLIPSSLPSTR
                     PLSRDAMAALLAEARERAGATPDPWIEADLLAIEQRAAMLGVLRDTGVEARIAAGARP
                     PVPVGAERAGDRISNGMNTRLGADLGGSLGALLAWGYRPGLRYPMGESDDLDLVARAA
                     YGTLVGAGLAVTIGRESMWWGPGFRGALLLTDNAAPFDLVRVETDRPVRLPWVGPLSA
                     HLFVTRLESDRLAIPHPYLAGLRLVIRPWSRLEFGLSRTAMFGGEGRPVTLDLIWDVI
                     RARGENSASNPGNQIAGFDATVRIPWRAQPVALYVEWAGEDEANGWPSHPAAVVGAYL
                     PRILGSPRWTLRAEAADDALADVPGIWYRHALYRSGYTYKGAVIGHPMGTDARMTSVE
                     LTHRVTPDWSVAALYDGLQRNVYAVDESSGRSLGARISRDQGTDVAIAEYRYARFEDP
                     TAPHRSDHLLTVSYRRAW"
     gene            3356..>4580
                     /locus_tag="AC16BC_06535"
     CDS             3356..>4580
                     /locus_tag="AC16BC_06535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR3241314.1"
                     /translation="MPTLEDIERKRAVIGIIGLGYVGLPLAVEFAKAGFTVIGIDLDA
                     DKVRSLARGRSYIQDVPDEDIKYIFKVGRMTATTDFSALRRVDAVSICVPTPLRKTKD
                     PDISYIAAATDQVAKYLPKGQLVVLESTTYPGTTNEIVLSELAKKGKRVGKDFFLAFS
                     PERVDPGNPKFHTRNTPKIIGGVTPQCTALAVALYRHAVDQVIPVSSTQAAEMVKLLE
                     NTFRAINIGLANEAALICRTLGIDVWEVIDAASTKPFGFMPFYPGPGLGGHCIPIDPL
                     YLSWKLKAFNYNARFIELASEINTKMPEHVVEIITEALNRAKKSVNGAKLLVAGVSYK
                     RDVDDVRESPALDIIRLLESKGATVAYTDPHVPKLDVGNGRVLKSAKPDRVSGYDAVV
                     IVTDHHAFDYKAMVKH"
BASE COUNT          779 a         1476 c         1578 g          747 t
ORIGIN      
        1 cttgctcaag gacatgcggg tcaaggatct ggtgttcgaa ggcgggctca cccgcgaatc
       61 cgaactgggc caggccgagt tgatccggct ggagaacggc gaggagaccg ggcaggtgcg
      121 gtgcgcggtg gtgcccatcg acctggccaa ggccatggcc gacgacccga cgcacaacgt
      181 gcccttgcgc aaggacgacg agttgttggt caagcaggtg ggcgaactca aacgcgagta
      241 ccacgtggcc gtcgaaggcc aggtgcggtt tcccggcagt tacgcgatcc gcgcgggcga
      301 gcgcatcagt tccttgatcc ggcgcgcggg cggcctgacc gacaaggcgt tcctgcgcgg
      361 cgcggtgttc acgcgcgaga gcgtgcgaat cgagcaacaa aaccggctgg aagacctgac
      421 cgcctcgtac acgcagcggg tcacggccga ggccgcggcc gtggccacca gcggattgtc
      481 caaggaagag accgaaaccg cgcggttcga actgagcgcg cgccagaact cgatcaagct
      541 gctgacccag gacaccgcgc tcggccgggt ggtggtgcat cttcgcgacc ccgacgcgct
      601 cgagggcacg ccggaggatc tggtgctgga ggatggggac aaactcgtga tcccgcaggt
      661 cccgtcgtcg gtcacggtgc tgggcacggt gcgaaatccc accgcgatcg cggcgcatga
      721 aggcgggtcc gtcaaggagt acatccgcct ggctgggggc tacctgccgg aggcggatcg
      781 caaggcggtc tacgtggtca aggccgacgg gtcgtcggtc ggttcggatc aggtcaagac
      841 catcgaagcc ggcgactcga tcctggtgcc gccgcaaatg gatcccaagt accggccgct
      901 gccgttgtgg cgcgacgtcg ccacgatcgt ggggcagttc gcgatcgcga tcgcggcgtt
      961 gaccgtcatc ttctgatgac gcggacgcgc gaactcatgg tggacgcgtc cgaaccggcc
     1021 gcggatcagc ggccggagga cgaacccacg atcctggatt acgcccgcgt gctctggggc
     1081 gggcggtggc tgatcctggt catcacgctg ctcgtcacgg ccgcggccgt ggggatcagc
     1141 ctcaccatgt ccaagtccta caccgcgaag tccacgttga tgccgttggg cctggaccgc
     1201 ggcggggggt tgggcgcgct ggccggcgcc ctctcgatcg agaaccccgg catgaagctc
     1261 atggccgtgc tgcagagccg gaccgtggcc gaagacgtgg tcggcaagtt gaacctggag
     1321 ccggagttcg cggtggggcg cgatcgcgcg cccacccacg ccgaggtgat cgaatccttt
     1381 caaaagcgcg tgctcaaggt cacggccagc cagggcatga tcgtggtcaa ggcggcgtgg
     1441 cgcgaccccg agctgtccgc gaagatcgcc aacaccgcgg tcctgtccgc ggcgcgctat
     1501 ttgaacgagc actccatcag caccagtttt cagatcctgg acgaggccgt ggctccggcc
     1561 aagccatcga gccccaagat cgcgctgaac accgcggccg cgttcgtgtt gtcggggttt
     1621 gccgcggtct gtctcgtgtt catccggcaa tatctccggg aagtccgtcg cgccgaatcc
     1681 gcgcgcgccg cagcggtcgc tccgtccgga ggcgcctcgt cgggccccgc gtaagccggc
     1741 cgcttctggc cagcgtgctc gcggcctgct gcgcgtccgg cgcgctcgcg ggcgagccgc
     1801 atctcacggt cggcgatccg gtctacgagc gcatcgaccg cctggccgca ttgggcctga
     1861 tcccgtcgag ccttccgagc acgcggccgt tgagtcgcga cgcaatggcc gcgttgctgg
     1921 ccgaggcccg ggagcgggcg ggcgcaacgc ccgatccgtg gatcgaggcg gatctcctgg
     1981 cgatcgaaca gcgcgccgca atgctcggcg tgctccggga cacaggggtc gaggcgcgga
     2041 tcgcggccgg cgcgcggccc ccggttccgg tcggcgcgga gcgagccggg gaccggatca
     2101 gcaacggaat gaatacccgg ctgggcgcgg accttggcgg ttcgctcggg gccctgctgg
     2161 cctgggggta tcgccccgga ctccggtatc ccatgggaga gtccgacgac ctcgacctgg
     2221 ttgcgcgcgc cgcgtacggg accctggtcg gcgcgggcct ggccgtcact attggccggg
     2281 agtccatgtg gtggggaccg gggtttcgcg gtgcgctgct gctgaccgat aacgcggcgc
     2341 cgttcgatct ggtgcgggtg gagaccgatc gcccggtgcg cctcccctgg gtcgggccgt
     2401 tgtccgcgca tctgttcgtg acgcgactgg agtccgaccg cctggcgatc ccgcacccgt
     2461 atctggccgg gcttcgcctt gtcatccgtc cgtggtctcg gctagaattc ggactttcgc
     2521 ggaccgccat gttcgggggc gagggccgtc cggtcacctt ggatctgatc tgggacgtga
     2581 ttcgcgcgcg gggcgagaac tccgcgagca atcccggcaa ccagatcgcg ggattcgacg
     2641 ccacggtgcg catcccgtgg cgggcacagc cggtcgcgct ctatgtggag tgggccgggg
     2701 aggatgaagc gaacggctgg ccgtctcacc cggccgcggt ggtgggggcg tacctgcccc
     2761 ggatcctggg atcgccccgc tggacgttgc gggcggaggc ggcggatgac gcgctcgcgg
     2821 acgtgcccgg catctggtat cgacacgcat tgtaccggtc cggctacacg tacaagggcg
     2881 cggtgatcgg ccaccccatg ggaacggatg cgcggatgac gagcgtggaa ctgacgcacc
     2941 gggtgacgcc ggactggagc gtggccgcgt tgtacgacgg cctgcagcgc aacgtgtacg
     3001 cggtggacga gtcctcgggc cggagcctgg gcgcgcggat cagccgcgac caggggactg
     3061 acgttgcgat cgccgagtat cgctacgcgc ggttcgagga cccaaccgcg ccgcaccgga
     3121 gcgatcacct gctcaccgtc tcctaccgcc gcgcgtggta gaaggttgcg aacaaaacct
     3181 actcagaaag tgtctcggac tgaggcgagc aggatggcaa tttttgctat aggctgctca
     3241 aaaagccaca gctgccccct tcggggactt cccgcatttc tttttttgcg gtgggtcgca
     3301 aggccgcaga tgggtttttt cagcagcctc tatctaatag gaataaggag agacagtgcc
     3361 taccctcgaa gacatcgaac gtaaacgcgc agtcatcggc atcatcggcc tgggatacgt
     3421 ggggctgccc ctggccgtgg agtttgcgaa ggccgggttc acggtcatcg gcatcgactt
     3481 ggatgccgac aaggttcgca gtttggcgcg cggccgatcc tatatccaag acgtgccgga
     3541 tgaggacatc aaatacatct tcaaggtcgg gcgcatgacc gcgaccacgg acttctccgc
     3601 gttgcgccgg gtcgacgcgg tcagcatctg cgtgcccacc ccgctgcgaa agaccaagga
     3661 tccggatatc tcgtacattg ccgcggccac cgaccaggtc gccaagtatt tgcccaaggg
     3721 acaactcgtg gttctggaga gcaccacgta cccgggcacc accaacgaaa tcgtgttgtc
     3781 cgaattggcc aagaagggca agcgtgtagg gaaggacttt ttcctcgcgt tttcccccga
     3841 gcgggtggat cccggcaacc ccaagttcca cacccgcaac acgcccaaga tcatcggcgg
     3901 ggtcaccccg cagtgcaccg cactggccgt ggcgctctat cgccacgcag tggaccaggt
     3961 gatcccggtc agctccacgc aggccgcgga aatggtcaag ttgctcgaga acacgttccg
     4021 cgccatcaac atcgggctcg ccaacgaagc cgcgttgata tgccgcaccc tgggcatcga
     4081 cgtgtgggag gtcattgacg cggcgtccac caaacccttc gggttcatgc cgttctaccc
     4141 cggccccggc ctgggcgggc actgcatccc gatcgacccg ttgtatctct cgtggaagct
     4201 caaggcgttc aactacaacg cgcgcttcat cgagctggcg agcgagatca acaccaagat
     4261 gccggaacac gtggtggaga tcatcaccga ggccttgaac cgggccaaaa agagcgtgaa
     4321 cggggccaag ctgctggtgg cgggcgtgtc gtacaagcgc gacgtggacg acgtgcgcga
     4381 gtcgcccgcg ctggacatca tccgattgct cgaatccaag ggcgcgaccg tggcgtacac
     4441 ggacccgcac gtgcccaagc tggacgtggg caacggccgc gtgctcaagt ccgccaaacc
     4501 cgaccgcgtg tccgggtacg acgcggtggt catcgtcacc gaccaccacg cgttcgacta
     4561 caaggccatg gtcaaacacg
//
LOCUS       DBNGTT010000293         2890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2490265, whole genome shotgun sequence.
ACCESSION   DBNGTT010000293 DBNGTT010000000
VERSION     DBNGTT010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2490265"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="era"
                     /locus_tag="AC16BC_06540"
     CDS             <1..793
                     /gene="era"
                     /locus_tag="AC16BC_06540"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR3241315.1"
                     /translation="VSPVPQTTRTSVLGILRRAGVELIFVDTPGLHKARHLLNEAMIR
                     AARAALDESDVVCLVIDATAGWRPGDRAVLNIVTARRAPVVLVINKVDRVKKPALLPL
                     IDECRKAHPFAQIIPISAQKDEEFERLIQALAALLPAGDAQYPADEYTDQTTRAMAAE
                     LIREAILVRTREEVPHAVAVRIEEFTEGAANGLVSIRAQVLVEKESQKAILIGAGGSM
                     MKQVGEQARLEIERLLGSKVYLSLWVTVEPQWRQDARQLGELGYA"
     gene            790..2151
                     /gene="mgtE"
                     /locus_tag="AC16BC_06545"
     CDS             790..2151
                     /gene="mgtE"
                     /locus_tag="AC16BC_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3241316.1"
                     /translation="MTDATFDMIVETFRKFLRRGAITHLSNMANKMRPADLAQVIRHL
                     STVQERRTVFELLRDVKAKAAVLSESEPQAIGELLSDMPPHDVVVVLRELASDDVADI
                     LGNMPEEKAQEILHLMKADQSSEVKDLLQYPEETAGGIMTTDFVSLHEDTTVKDAIAH
                     LQETSAKQMVFYLYVTDHAGRLVGVVSLRQLLVVSPTTPLKKIMTTDVISVVTDMDQE
                     EVSKLVAKYNILAIPVVDKDNILVGIITVDDVVDVIREEATEDILKLAGATEADLLQM
                     SSIRAAKMRMPWLLTSLVGGMITGVFLWLFRPAIQHVIALASFIPVITAMGGNVGLQT
                     STLVVRGLATGRIESAEIRAVFLKELTVGLMMGTICGFIVGLVAQLWHGPPMLGIVVG
                     VSMFCAITVAALMGSLMPMLLRRLGIDPAISSGPFVTAANDITGLVIYLGLATILLER
                     LTG"
     gene            2161..>2890
                     /gene="recO"
                     /locus_tag="AC16BC_06550"
     CDS             2161..>2890
                     /gene="recO"
                     /locus_tag="AC16BC_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR3241317.1"
                     /translation="MLVDCQAIVLAGQKLGEADTLVTLLTMERGLVKGVAKGARRLKS
                     RFGGAIQPFTYGQALLVERRAAGLRSLAHFDSIHSFQRVREDFEALSAAAAVVNGARR
                     LLPDEQPSADAFTLLLKALRSVEAGKDLDRVVLWALLALLQAAGYQPRVDRCLVGRHG
                     ARPTDTAGRWWFVPHLGGAVCPSCGVGALAGSNGQHAGLPMTPGVHALLRQALRMPPS
                     ARHRVSAGAELIQEARGLLEACVAE"
BASE COUNT          472 a          911 c         1010 g          497 t
ORIGIN      
        1 cgtctccccg gtgccgcaga cgacgcgcac gagcgtgttg ggcatcctcc gccgcgccgg
       61 cgtggaattg atcttcgtgg acacgcccgg gctccacaag gcgcgtcacc tgttgaacga
      121 ggccatgatc cgcgcggcgc gcgccgcgct cgatgagtcg gacgtggtct gcctggtcat
      181 cgacgccacg gccggatggc gtcccgggga tcgcgccgtc ctcaacatcg tgacggcgcg
      241 gcgcgcgccc gtggtgttgg tcatcaacaa ggtggaccgg gtcaaaaagc ccgcgctgtt
      301 gccgctgatc gacgagtgcc gcaaggcgca cccgttcgcc caaattatcc cgatttcggc
      361 gcaaaaagac gaggagttcg agcggttgat ccaggcgctg gccgccctgc tgcccgccgg
      421 cgacgcgcag tatccggcgg acgaatacac cgatcagacc acgcgcgcca tggccgcgga
      481 actgatccgg gaagcgattc tggtgcgcac gcgcgaagaa gtgccccacg cggtggcggt
      541 gcgcatcgag gagtttaccg aaggcgccgc caacgggttg gtctccatcc gcgcccaggt
      601 gctggtggag aaggagtcgc agaaggccat cctgatcggc gcgggcggct ccatgatgaa
      661 acaggtcggc gagcaggcgc gactggaaat cgaacggttg ctggggtcca aggtgtattt
      721 gtcgttgtgg gtcaccgtgg agccgcagtg gcggcaggac gcgcggcaac tcggcgagct
      781 gggatacgca tgaccgacgc cactttcgac atgatcgtgg agacgttccg gaagttcctg
      841 cgccggggcg cgatcacgca cctctccaac atggccaaca agatgcggcc cgcggatctg
      901 gcgcaggtga tccgccacct gtccacggtg caggaacggc ggaccgtctt tgaactgctc
      961 cgcgacgtca aggccaaggc cgcggtcctg agcgagagcg agccccaggc gatcggcgag
     1021 ttgttgtccg atatgccgcc gcacgacgtg gtcgtggtgc tgcgcgaact ggcctccgac
     1081 gacgtggccg acatcctcgg gaacatgccc gaggaaaagg cgcaggaaat cctgcacctg
     1141 atgaaggccg accagtcgag cgaggtcaag gacctgctgc aatatccgga ggagaccgcg
     1201 ggcggcatca tgaccaccga cttcgtgtcg ctgcacgagg acacgacggt caaggacgcg
     1261 atcgcgcacc tccaggagac gtcggccaag caaatggtct tttacctgta cgtcacggac
     1321 cacgcgggcc ggttggtggg ggtggtctcg ttgcggcaat tgctggtggt ctcgccgacc
     1381 acgccgctca aaaaaatcat gaccacggac gtgatcagcg tggtgaccga catggatcag
     1441 gaggaggtct ccaagctggt cgccaagtac aacatcctgg cgatcccggt cgtggacaag
     1501 gacaacatcc tggtcggcat catcaccgtg gacgacgtgg tggacgtcat ccgcgaggaa
     1561 gcgaccgagg atatcttgaa gctggcgggc gccaccgaag ccgatctgct ccagatgtcc
     1621 agcatccggg ccgcgaagat gcgcatgccc tggttgttga cgagcttggt gggcgggatg
     1681 atcaccgggg tctttctgtg gctcttccgg cccgcgatcc aacacgtgat cgcgctggcc
     1741 agcttcatcc cggtgattac cgcgatgggc ggcaacgtgg ggctgcagac gtccacgctg
     1801 gtggtccgcg ggttggcgac cgggcgcatc gagtccgcgg agattcgcgc cgtgtttctc
     1861 aaggaactga ccgtcggact catgatgggc acgatctgcg gcttcatcgt cgggttggtg
     1921 gcccagctct ggcacgggcc gcccatgctg ggcatcgtgg tcggggtgtc catgttctgc
     1981 gcgatcacgg tcgccgcgct gatgggctcg ctgatgccca tgttgctccg acggctcggg
     2041 atcgatcccg cgatctcgtc aggccccttt gtcaccgcgg ccaacgatat caccgggctc
     2101 gtgatctacc tgggcctcgc cacgatcctc ttggaacgtc tcaccggttg acgcccccgc
     2161 atgctggttg attgtcaggc cattgtgttg gcgggccaga agctggggga ggccgacacc
     2221 ctggtgacct tgctgaccat ggagcgcggt ctggtcaagg gcgtggccaa gggcgcgagg
     2281 cggctcaaga gccggttcgg cggagcgatc cagccgttta cctacgggca agctctcctc
     2341 gtcgagcgcc gggcggcggg gttgcggtcc ctggcccact ttgattcgat ccattcgttt
     2401 caacgcgtgc gggaggactt cgaggcgctg agcgcggccg cggccgtggt caacggggcg
     2461 cggcgactcc tgcccgatga acagccctcc gcggacgcct ttaccctgct tctcaaggcg
     2521 ctccgatccg tggaggcggg gaaagatctg gaccgcgtgg tgttgtgggc cctgctcgcg
     2581 ctgttacagg ccgcggggta ccaaccccgg gtggaccgtt gcctggtcgg gcgccacggc
     2641 gcgcggccga ccgacaccgc ggggcggtgg tggttcgttc cccacctggg aggcgcggtc
     2701 tgcccttcgt gcggggtggg cgcgttggcc gggtccaacg gccagcacgc ggggctcccg
     2761 atgacgcccg gcgttcacgc cttgctccgt caggcgcttc ggatgccgcc ctcggccagg
     2821 caccgcgtat cggccggcgc cgaactgatt caggaggcca gggggctgct ggaggcctgc
     2881 gtcgccgaac
//
LOCUS       DBNGTT010000294         2172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1469416, whole genome shotgun sequence.
ACCESSION   DBNGTT010000294 DBNGTT010000000
VERSION     DBNGTT010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1469416"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="AC16BC_06555"
     CDS             complement(<1..222)
                     /locus_tag="AC16BC_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241318.1"
                     /translation="MSVVWIRGISNRIGFALLGVLVFALTLPSPTLAVMTNPRPIPAN
                     GGLVTPPGAANHVPDHAPGEVLVKFKKDTP"
     gene            complement(333..1670)
                     /locus_tag="AC16BC_06560"
     CDS             complement(333..1670)
                     /locus_tag="AC16BC_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HIR3241319.1"
                     /translation="MGLEIAPPDLVMTAVDALSASVAQGDYLYVSNTVQNQGGSNAGT
                     FTIAFHLSVDAVYGGTDDVTLSATRSVTSLAVGGTSSAAYLVTVPSTTPIGTYYVCAM
                     ADSGGTIAEGTAEGNNTLCGASTIDVTAATAVGRLVNISTRAFVGTGTSAAVGGFIVS
                     GTGTKQVLLRGFGPTLMSFGITGALANPTLDLYWDDDNNPSTAAILVLTNNDWGTPLA
                     ACNAPVVSCGTPQNIADTGMSADSYAPSNPNRGLDAALLLTLPPGTYTATLRGVNNGT
                     GVGLIGVDDVDTNQTARLVNISTRAFVGTGTSAAVGGFIINGTNNKQVLLRGFGPTLS
                     SFGITGTLANPTLDLYWDDDNNPSTAALLVVTNNDWGTALASCPAPVVACGTPTDIEN
                     TGMSANSYAPTNPNRALDAALLVTLPPGTYTATLRGVSNGTGVGLIGVDEIGP"
     gene            1762..1977
                     /locus_tag="AC16BC_06565"
     CDS             1762..1977
                     /locus_tag="AC16BC_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241320.1"
                     /translation="MDVTAPLAGTVTVPGLKLPVRPAFEALNVKATDPVNVPTDVTVT
                     VYVVEPPALMVRLAGAAVTVKLDSNAR"
     gene            complement(2043..2150)
                     /locus_tag="AC16BC_06570"
                     /pseudo
     CDS             complement(2043..2150)
                     /locus_tag="AC16BC_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT          399 a          693 c          685 g          395 t
ORIGIN      
        1 gggcgtgtcc ttcttgaact tgaccagcac ctccccaggt gcgtggtccg gaacgtggtt
       61 tgccgcgccc ggcggtgtca caaggccccc attggccggg atgggtcgcg ggttcgtcat
      121 aacggccagg gttggagatg gcagggtaag ggcaaacacc aataccccaa gaagcgcgaa
      181 cccgattcga ttgctgatac cgcgtatcca gacgactgac atactccccc cttcgcaccg
      241 tgacgtgaga cggcgccaca ctcggcaatc ggacgcggcg cgtctctgtt agagaccgat
      301 caaataccgt tcagaatcat cattgagggc atctacggcc cgatctcatc caccccaatc
      361 aagcccacgc ccgtgccgtt acttacgccc cgcagcgtcg ccgtataggt gcccggcggc
      421 agggttacca acagcgccgc atccagcgcc cgattggggt tcgtcggcgc gtaggagttg
      481 gccgacatgc cggtgttctc gatgtcggtc ggtgtgccgc acgcgactac cggggccgga
      541 cacgacgcga gggccgtgcc ccagtcgttg ttggtgacca ccaggagcgc cgcggtgctc
      601 gggttgttgt cgtcgtccca atatagatca agcgtgggat tggccaaggt gcctgtgatc
      661 ccaaagctgc tcaaggtcgg cccgaagcct cggagcagga cttgtttgtt gttggtccca
      721 ttgatgatga acccgcccac tgcggcactg gtccccgtcc ccacaaaggc ccgggtggag
      781 atgttgacca accgagcggt ctgattggta tccacatcat ccaccccgat caggcctacc
      841 ccggtgccgt tgttcacgcc gcgtaacgtg gcggtatacg tgcccggcgg cagggtcagc
      901 aacagcgccg catccagtcc tcggttggga ttgctcggcg catagctatc cgccgacatc
      961 ccggtgtcgg caatgttctg aggggttcca caggagacga cgggggcgtt gcacgcggcg
     1021 agcggtgtac cccagtcgtt gttggtcagc accaggatcg ccgccgtgct gggattgttg
     1081 tcatcatccc aatacagatc caaggtcgga ttggccagtg cccccgtgat tccaaaactc
     1141 atgagcgtgg gcccgaagcc acggagcagg acctgtttcg tcccggtccc gctgacgata
     1201 aagccgccaa ctgccgcgct ggtccccgtg cccacaaagg cccgggtgga gatgttgacc
     1261 aaacggccga cagccgtggc cgccgtgacg tcgatcgtcg acgctccgca aagcgtgtta
     1321 ttgccttccg ccgtaccttc ggcgatcgtc cctccgctgt ccgccatggc gcagacgtag
     1381 taggtgccaa tcggcgtcgt ggatgggacg gtgaccaggt acgcggcgct gctggttccc
     1441 cctacggcca gagaggtcac ggaacgcgtc gcagatagcg tcacgtcatc cgttccgcca
     1501 taaaccgcgt ccaccgagag atgaaacgcg atcgtaaagg tgccggcgtt cgagccgcct
     1561 tgattctgaa cggtattgga aacatacaga taatctccct gcgctacgct cgccgacagc
     1621 gcgtccaccg cggtcatcac cagatccggc ggcgcgatct ccaaccccac cattgtggaa
     1681 tgcgtttcgc tcgacactgt gccgcggatc gtgatcgaat aatttccggc agtcatcccg
     1741 gcgccggcgg aaaccgtcag cgtggacgtg acggccccgc tggccggaac cgtcacggtc
     1801 cccgggctga agctgcccgt caggcccgca ttcgaggcgc tgaacgtcaa ggcgacggat
     1861 cccgtgaatg tcccgactga cgtgaccgtg accgtgtatg tggtggagcc gcctgcgttg
     1921 atggtccggc ttgcgggcgc ggcggtcacg gtgaagttgg acagcaacgc gcgctgaacg
     1981 ttcaatcgac cgcccgtaac cgtgactccc gtcagggcgg gaatgggatc caccgaagcc
     2041 aggatcgcgt ctcggacctg cgcgaccgtg taggatggaa actgcgagag gatgagcgcg
     2101 gccgcgcccg aaacgtgcgg cgcagccatt gacgtgccgc tgaccgtcaa atagcgcgag
     2161 gggtcgcagt ac
//
LOCUS       DBNGTT010000295         3299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3258095, whole genome shotgun sequence.
ACCESSION   DBNGTT010000295 DBNGTT010000000
VERSION     DBNGTT010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3258095"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="AC16BC_06575"
     CDS             complement(<1..1132)
                     /locus_tag="AC16BC_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR3241321.1"
                     /translation="MEQMAIMFALACAAAAIVYGAVSAKWVVGLSAGNARMQEIAQAI
                     QEGAKAYLNRQYTTIAMVGAVLFVALLVALDWKTAGGFAIGAVLSALAGYVGMNVSVR
                     ANVRTAEAARGGLAAAFNVAFRGGAVTGLLVVGLGLLGVAGYYWFLHATTPEGQAVNI
                     TPLVGLGFGGSLISIFARLGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYAVTIISAMLLGALAFKGAAINSALVYPLVLGGISIIASIIG
                     TFFVRLGSNNNIMGALYKGLIWSALLSAAAFYPVTSWMMSGTDGPSVMNIYLSSLVGL
                     VVTALLVMITEYYTSTAYKPVRDIAQASTTGHATNVIAGLAVG"
     gene            complement(1249..1821)
                     /gene="pth"
                     /locus_tag="AC16BC_06580"
     CDS             complement(1249..1821)
                     /gene="pth"
                     /locus_tag="AC16BC_06580"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3241322.1"
                     /translation="MKCVVGLGNPGAQYAATRHNIGFLVADAFAAAHAIQLRALGGLV
                     VAGRGALAGEDVWIVEPQGFMNRSGAAVQWLLDETGAGPDALLIVHDDLDLPLGQLRF
                     KRKGGHGGHNGVRSVIETLGTDEFLRLKVGIGRPPTGSDTVDYVLEPFSRDEDPAVAG
                     VIERARAAVTAALTEGVGAAMNRFHASAAE"
     gene            complement(1977..2705)
                     /locus_tag="AC16BC_06585"
     CDS             complement(1977..2705)
                     /locus_tag="AC16BC_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR3241323.1"
                     /translation="MQRFELTVEQRTTAGKGVARTLRRAGRIPAVLYSGGKSTMLSLN
                     PADLSKLLHSAARENTLIDLKVGGTRDKERLAILREVQHDPLTGKPLHIDLFEVALDR
                     AIRLKVHVEITGSTPLGVKEGGLLQHGAREVEIECLPSLIPDAVRVDASPLKTGEGIH
                     LRDLQLGEGIRVLGDPDTVIVSVTMPMSEEKLAEILAGAPKEVKEPEVLTAKKAEGEA
                     AAGAKPEAKAEAKGGKEEKKEGKK"
     gene            complement(2800..>3299)
                     /locus_tag="AC16BC_06590"
     CDS             complement(2800..>3299)
                     /locus_tag="AC16BC_06590"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3241324.1"
                     /translation="TPVLLEHFRQVEIADLVIVSPDAGGVERARAFAKRLKANLAIID
                     KRREGPNQTKLMNIIGDVAGRNVLLLDDMIDTAGTIAQGAHALREKGARTILAGCTHP
                     VLSGPALDRINASPIDTLVVTNTLPLNGKEEACRKIVSLTVAPLLGEAMKRIHEEESV
                     SSLFV"
BASE COUNT          539 a         1181 c          993 g          586 t
ORIGIN      
        1 taccgacggc caggcccgcg atcacgttgg tggcgtgccc ggtggtggac gcctgcgcga
       61 tatcgcgcac cggcttgtac gcggtggagg tgtagtactc ggtgatcatc accaataacg
      121 cggtcacgac cagcccgacc agcgaggaca gatagatgtt cattacgctc ggaccatcgg
      181 tgccgctcat catccaggac gtcaccgggt agaacgccgc ggccgagagg agcgcgctcc
      241 agatcaggcc cttgtacaac gcgcccatga tgttgttgtt cgagcccagc cgcacgaaga
      301 acgtgccgat gatggacgcg atgatcgaga tcccgccgag gaccagcggg tacaccaacg
      361 cggagttgat tgccgcgccc ttgaacgcga gcgcgcccag caacatggcg gagatgatgg
      421 tgacggcgta ggtttcgaac aggtccgcgg ccatgcccgc gcaatccccc acgttgtcgc
      481 ccacgttgtc cgcgatcacg gccgggttgc gcggatcgtc ttccggaatc ccggcctcga
      541 ccttacccac caggtccgcg cccacgtccg cggccttggt gaaaatgccg ccgcccagac
      601 gcgcgaaaat actgatcagg ctgccgccga atccgagacc gaccagcggg gtgatgttca
      661 cggcctggcc ttccggcgtg gtcgcgtgca ggaaccagta gtagcccgcc acgcccagga
      721 gcccgaggcc gaccacgagc aggcccgtga ccgcgccgcc gcgaaacgcc acgttgaacg
      781 cggccgccag tccgccgcgc gcggcttccg cggtgcgcac gttggcccgc accgacacgt
      841 tcatgcccac gtacccggcc agggccgaga gcacggcgcc gatcgcaaag ccccccgcgg
      901 tcttccagtc cagggccacg agcagcgcca cgaacaagac cgcgccgacc atggcgatcg
      961 tggtgtactg ccggttcagg taggctttcg cgccttcctg gatcgcttgc gcgatctcct
     1021 gcatgcgcgc gttgcccgcc gacaacccca cgacccattt ggcgctcacc gcgccgtaga
     1081 cgatcgccgc cgcggcgcac gccagggcga acataatcgc catctgctcg attcccatca
     1141 ctccgctcct tctcggtaat agataccggc cgtgccgggc cgggatcttt tggacgtccc
     1201 gggcccgctc acgtcataaa aaaaagcgac cgtagtcgcg tcctcttgct actccgccgc
     1261 cgatgcgtga aagcgattca tcgcggcgcc caccccttcg gtcaacgccg cggtcaccgc
     1321 agcgcgagcc cgttcgatca cacccgcgac cgccgggtcc tcgtctcggg aaaacggctc
     1381 caacacgtaa tccacggtgt cggacccagt tggcggtcgg ccgatgccca ccttcaaccg
     1441 cagaaactca tcggtgccca acgtctcgat gaccgaccgg acgccgttgt ggccgccgtg
     1501 cccgcccttg cgcttgaagc gcagctggcc caggggaagg tccaggtcgt cgtgcacgat
     1561 cagcagggcg tcgggcccgg cgccggtctc gtccaagagc cattgcaccg cggcgccgct
     1621 gcgattcatg aaaccctggg gctcgacgat ccagacgtcc tcgcccgcca aggcgccgcg
     1681 cccggccacc accaggccgc ccaaggcacg caattgaatc gcgtgcgccg cggcaaacgc
     1741 gtccgcgacc agaaacccta tgttgtggcg ggtggcggcg tactgcgcgc cgggattgcc
     1801 cagccccacc acgcacttca cggaccgtta ctgagcctgt cctggacagg ccatctgcag
     1861 cgttgcctct tgcgctccgg tgctcacgta cgcatccagt acgctccgct gctcagcggc
     1921 gccttgcatc cgacctgtcc tgaccagctc cactttcgcg gttactggga caggctctat
     1981 ttcttgcctt cttttttctc ttccttgccg cccttggctt cggccttggc ttccggcttg
     2041 gcgccagccg cagcttcgcc ctccgccttc ttggcggtca gcacttcggg ttccttgacc
     2101 tccttgggcg cgccggccag gatctcggcc aacttctcct cggacatcgg catggtcacg
     2161 gacacgatca ccgtgtcggg atcgcccagc acccggatgc cctcgcccaa ctgcaggtcc
     2221 cgcaaatgaa tgccctcgcc ggttttcaac ggcgacgcat ccacccgcac cgcgtccgga
     2281 atcagggacg gcagacactc gatctcgact tcgcgcgccc cgtgctggag caggccgcct
     2341 tctttcacgc ccagcggcgt ggagcccgtg atctccacgt gcaccttcag gcgaatcgcg
     2401 cgatccagcg ccacctcgaa caagtcgatg tgcaggggtt tgccggtcag tgggtcgtgc
     2461 tgcacctcgc ggagaatcgc caggcgctct ttgtcgcgcg tgccgcccac cttcaagtcg
     2521 atcagggtgt tttcacgcgc ggccgagtgc aagagtttgg acaggtccgc cgggttcagg
     2581 ctcagcatgg tggacttccc gccgctgtac aggaccgcgg ggattcgccc ggcccgccgc
     2641 agggtccgcg ccacgccctt gcccgccgtt gttcgctgtt caaccgtcaa ttcgaaacgt
     2701 tgcatgccct actccttcct ctcttcgttc gatctcgctc cccccaccct ccccctcaag
     2761 gggggagggc aagggagggg gtggactttc gcatgagttc tacacaaata acgaactgac
     2821 cgactcttct tcatgtatgc gcttcatcgc ctcgcccaac aacggcgcga cggtcagcga
     2881 cacgatcttg cgacaggctt cttccttgcc gttcaagggc aaggtattgg tcacgaccag
     2941 ggtatcgatg ggcgaggcgt tgatgcggtc gagcgcggga cccgacaaca cggggtgggt
     3001 gcacccggcc aggatcgtgc gcgcgccctt ttcccggagc gcgtgcgcgc cctgcgcgat
     3061 ggtacccgcg gtgtcgatca tgtcgtcgag cagcaacacg ttgcggcccg ccacgtcgcc
     3121 gatgatgttc atgagtttgg tctgattcgg cccctcgcgc cgtttgtcga tgatcgccag
     3181 gttggccttg agccgtttgg cgaacgcgcg cgcgcgctcc acgcctccgg cgtccgggga
     3241 gacgatcacc agatccgcga tttccacctg ccggaagtgc tccagcaaca ccggcgtgg
//
LOCUS       DBNGTT010000296         9075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2363973, whole genome shotgun sequence.
ACCESSION   DBNGTT010000296 DBNGTT010000000
VERSION     DBNGTT010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2363973"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC16BC_06595"
     CDS             <1..449
                     /locus_tag="AC16BC_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR3241325.1"
                     /translation="RRALRVNTESIGLQTGPFGAKGAWWIAVALYAGAIYALSAQSHP
                     FGIQSLPPRMDKLIHASVFGGFSLVLWMALRRSMPGLTAGALSVLAVVLTALCGLSDE
                     IHQAFVPGRAMEALDWAADVTGAALMQWVILFRARAQAVRRPGGAT"
     gene            446..1867
                     /locus_tag="AC16BC_06600"
     CDS             446..1867
                     /locus_tag="AC16BC_06600"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR3241326.1"
                     /translation="MTGAHPYAVILAGGSGTRFWPLSRRLAPKQLLTLTGDRTMLQET
                     VLRITPAVAPDRVMVITGRELSDEARRQLRVIGIPAVVVDEPVGRNTAPAIAVAAHRL
                     LAADPDAVMVVLPSDHVVSKPEAFLDALNAAIRAAADGWLVTIGIVPRRPETGYGYIQ
                     RGPALPGDRAYRVERFVEKPDRATAERYVADGGYLWNAGMFVWRADAIVDELRRHAPG
                     VEAAGAAIAEAMAQGGDPARLRTLYDGVESVSIDYAVLERSTRVAVVPAEFGWSDVGS
                     WAALDDVAGKDGRGNVISGRVVDVDSRESIVYAQDRLVATIGLDNLIVVDTPDATLVC
                     AKDRAQDVRKVVDALEAQRAQEHVVHKTVQRPWGTYTVLDEGPGYKIKRITVNPSSRL
                     SLQMHRQRSEHWVVVAGTARVTCGERAYDVKNNESTYIPPHTKHRLENVDPSVPLEII
                     EVQNGPYLGEDDIVRFDDDFGRK"
     gene            1886..3283
                     /locus_tag="AC16BC_06605"
     CDS             1886..3283
                     /locus_tag="AC16BC_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR3241327.1"
                     /translation="MSIYREYDIRGVVGTDLTFDVVERIGTAYGTTVRRAGGSTVAVG
                     RDVRDHSTPIRDRLAAGIMSAGVHVVDLGVCPTPLLYYSLFHLAVDGGVMITGSHNPP
                     EFNGLKMCVGREAIYGDAIQSLRRLVETGDRERGQGTRSDYAIIPPYLRELTDQFVSR
                     VKGSSRKPLRVVVDSGNGVAGLVAPALLRAVGCDVIELYSEPDARFPHHHPDPTVLDN
                     LRDLIAAVGRHHADLGIAYDGDADRIGVIDERGDVLWGDRLLALLAGPVLADRPGAPV
                     IAEAKCSQVLYDEITRRGGRAVMWKTGHSLIKARMKELSAPLAGEMSGHLFFADRYYG
                     FDDAMYASCRVVELLVRESRPLSALAAALPATVVTPEIRVDCPDEHKFGVVTRLQTFF
                     RDARAKSGEFPLPILEVIDVDGVRVRTPHGWGLVRASNTQPVLVLRFESDTEAHLKEI
                     RSAIESRVKECWFAS"
     gene            3265..4821
                     /locus_tag="AC16BC_06610"
     CDS             3265..4821
                     /locus_tag="AC16BC_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3241328.1"
                     /translation="MLVRLLILAAFLAVGCRAPASSDNTFRMVLSNEPPTLDWSLATD
                     SVSITVIENLMDGLTRFDQDLRPAPAVASSWDVSADGRRYLFHLRPDVRWSDGAPVTA
                     ADFEYAWKRLLDPKTAAQYAYFLYPVANAKAYNTGALPDASTVGVRALSATELEVVLD
                     RPIAYFPSLTTFMVTFPQRRDLIERYGDRWTDPGRLVTNGPFRLESWRHEYRLQLSAN
                     PTYYGGKPAIGHVAMYVVNERTTELTLYETGDLDYAQLPPEAIPAFEGSPDHRRLPIL
                     RGYYAGFNVTKPPFTDPRVRRAFALALDREEFPKILRGGERAARSWVPPGMFGYEPDI
                     GLPYDPERARRLLAEAGFPGGRGLPPIEYVFNTGPVNTLIAENLQAQWKRNLGVDVRL
                     DNVEWKVYLKRLERDAPGMFRLGWGADYPDPDNFLALFTKDSGNNHSRWANARYDALI
                     AEASSESDPARRLVMYREAQRLLTEEDVPIIPLFTHVQNMLVKPWVRGLDVNAMEIMS
                     LQGVSIGARP"
     gene            5148..6065
                     /locus_tag="AC16BC_06615"
     CDS             5148..6065
                     /locus_tag="AC16BC_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241329.1"
                     /translation="MIQVVVRRVVWGIGVLWLVATVTFALLRVVPGGPFDSEKRLPPE
                     ILANVEAKYHLDEPVWRQYARYLGGVARGDLGPSFKYFGRDVAAILMETFPVSCALGF
                     AALGIAVVAGVAFGIAAGSRPDGWMDRGGMLAATTGVALPNFVLGVALVLIVSHRLHW
                     LPPALWEGPRYVLLPALTLGLGPAAYIARLTRASLLDVARQDYVRTARAKGLSDRAVL
                     IAHQLPNALMPVVTLLGPLTAALITGSFVVEYLFAIPGMGRFFITAVTNRDYPLIMGA
                     TLLYAALLVAANLAADLVASRLDPRIRLE"
     gene            6076..6879
                     /locus_tag="AC16BC_06620"
     CDS             6076..6879
                     /locus_tag="AC16BC_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241330.1"
                     /translation="MTAVNVMVLIVVATAAVFAPVLAPYAYDTQDTAAIYAEPSSAHW
                     MGTDRLGRDLLSRVIYGTRTSLLIGLASALIALTLGAAYGGISGYAGGRTDQALMRSL
                     EALHALPDLLVIILLSTVLGRGVFSMIAAISLVGWMTVARLVRGEMLRLREQPFVEAA
                     RASGAGHARILLRHLLPQTIGLLIVTLTFRIPAGILAESTLSFVGLGIAPPQASWGSL
                     AGEGWSAIKFYPHVILFPSLVIFVTMLAFNLLGDRLQDILNPARSRRVG"
     gene            complement(6919..8109)
                     /locus_tag="AC16BC_06625"
     CDS             complement(6919..8109)
                     /locus_tag="AC16BC_06625"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3241331.1"
                     /translation="MRLTNCLGLTFGFGLCLTTLSITLSDPAHAAPLCPTPPQDQPMP
                     PVHLAPAFTGLNKPVALSSPGDGSGRVFIVEQSGTIKIRWPGRAGSKTFLDIRDRVSA
                     GGELGLLGLAFHPTFAENGRFFVNYTSRKGGLHSVISEFRASDAADEADARIERILLT
                     VPQPYSNHNGGDLAFGPDGMLYIALGDGGSGNDPLGNGQNLSTLLGKILRIDVDHRSD
                     ELAYGIPPDNPFMKRQGAAPEIWAYGLRNPWRFAFDPVTGWLFAGDVGQKAREEIDVI
                     RKGGNYGWNVMEGTVCTPGVNPRCKTSGFERPIFDYQRTEGTTVIGGRVYRGRAIPGL
                     CGAYVYGDFGTGRVWALRYDGATVIEQQKLLDTGRSISAFGEDDQRELYVVDYAGEVL
                     KLTP"
     gene            complement(8273..>9075)
                     /locus_tag="AC16BC_06630"
     CDS             complement(8273..>9075)
                     /locus_tag="AC16BC_06630"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3241332.1"
                     /translation="SCHVPALGFSNGQPVATGVRGQLGARNVPSLYNVAYRTQFFWDG
                     RVGSLEQQALEPIQNPIEMDQDLSQLVAELNAVPGYVKAFRAVFGTGPSADGIAQALA
                     AFERTIISNNSPWDRFMAGDDAALSESAKRGVRVFDEKAKCVTCHNGPNFTDNQFHAL
                     GLPKREGVPDDPGRYNVTLNDDDMETFRTPSLRQVADSAPYMHNGMFATLEDVLDFYV
                     KAEGHPAHRHPLITPLDLLTEQDKADLLAFIRSLSSDPIVVIPPPLPK"
BASE COUNT         1440 a         2874 c         3120 g         1641 t
ORIGIN      
        1 gaaggcgcgc tctccgcgtg aatacggaat cgatcggact ccagaccggg ccgtttggcg
       61 ccaagggcgc gtggtggatc gcggtggcct tgtacgccgg ggctatttat gccctttcgg
      121 cccaatcgca tccgttcggg attcaatccc tcccgccgag gatggacaaa ctgattcacg
      181 cctcggtctt cggcggattc tccctggtcc tgtggatggc gctccggcga tcgatgccgg
      241 gtctgaccgc gggcgcactc tccgtgctcg ccgttgtcct gaccgcgttg tgcggactgt
      301 ccgatgaaat ccatcaggcg ttcgtacccg ggcgggcgat ggaggcgctt gactgggccg
      361 cggacgtgac cggcgccgcg ctcatgcagt gggtgattct gtttcgcgcg cgcgcccaag
      421 ccgtacgccg ccccggcggg gccacgtgac gggggcgcat ccctacgccg tcatccttgc
      481 gggcgggagc ggaacacgat tctggccgct gtcacgacgc ctggctccca agcaactcct
      541 caccttaacc ggcgatcgca ccatgctcca ggagacggtg ctgcggatca cgccagcggt
      601 cgccccggat cgcgtcatgg tgatcacggg ccgggagctc tccgacgagg cgcggcgaca
      661 attgcgggtc atcgggattc ccgcggtggt ggtcgacgag ccggttggtc ggaacaccgc
      721 gcccgcgatc gcagtggccg cgcaccggct gttggcggcg gatccggacg cggtgatggt
      781 ggtgctgccg tcggatcacg tcgtatcgaa acccgaggcc tttctcgacg cgctgaacgc
      841 cgcgatccgg gcggccgcgg acgggtggct ggtcacgatc gggatcgttc cccgcaggcc
      901 cgagaccggc tacgggtaca ttcaacgcgg accggcgttg ccgggcgatc gcgcgtaccg
      961 tgtcgagcgg ttcgtcgaaa aaccggaccg cgccacggcc gagcgctacg tggcggacgg
     1021 ggggtatttg tggaacgctg gcatgttcgt gtggcgggcg gacgcgatcg tcgatgagct
     1081 gcgtcgccac gcgccgggag tcgaggcggc gggcgcggcc atcgcggagg ccatggccca
     1141 gggcggagac cccgcgcggc tgcggacgct gtacgacggg gtcgaatcgg tttcgatcga
     1201 ctacgcggtg ttggagcgct ccacgcgcgt ggccgtggtg cccgcggagt tcggatggag
     1261 cgacgtgggg agctgggccg ccctggatga cgtcgcgggg aaggacggcc gcggcaacgt
     1321 gatcagcgga cgggtcgtgg acgtggacag ccgggagtcg atcgtatacg cgcaggaccg
     1381 gctggtggcc acgatcgggt tggacaacct gatcgtggtg gacacgccgg acgccacgct
     1441 ggtgtgcgcg aaggaccggg cgcaggacgt ccgcaaggtg gtggacgcgc tggaagcgca
     1501 gcgcgcgcag gaacacgtgg tccacaagac cgtccaacgc ccgtggggca cctatacggt
     1561 gttggacgaa gggccggggt acaagatcaa acgcatcacc gtgaatccgt cgtcgcgtct
     1621 gagcctgcaa atgcaccggc aacgcagcga acattgggtg gtcgtggccg ggacggcgcg
     1681 ggtgacctgc ggggaacggg catacgacgt caagaacaac gagagcacgt acatcccccc
     1741 gcacaccaaa caccgtttgg agaacgtcga tccgtcggtt ccgctggaga tcatcgaggt
     1801 gcagaacggg ccgtacctgg gtgaggacga catcgtgcgg ttcgacgacg actttggccg
     1861 gaaataatat cggacgacgc gtggcatgag catttaccgc gaatacgaca ttcggggcgt
     1921 ggtcggcacg gacctgacgt tcgacgtggt ggagcgcatc ggtacggcat acggcaccac
     1981 ggtgcgccgg gccgggggat cgacggtcgc ggtggggcgc gatgtgcgcg atcactccac
     2041 gccgattcgg gaccgactgg ccgccgggat catgtccgcc ggcgtccacg tggtggacct
     2101 gggcgtgtgc cccacgccgt tgctctacta ctcgctgttt catctcgcgg tggacggcgg
     2161 ggtcatgatc acggggagcc acaacccccc ggagttcaac ggcctcaaga tgtgcgtggg
     2221 ccgcgaggcg atctacggcg acgcgatcca atcgttgcgt cggctcgtcg agaccggaga
     2281 ccgcgaacgc gggcagggga cgcgatcgga ctacgccatt atcccgccgt acctgcgcga
     2341 gttgaccgac cagttcgtct cgcgcgtgaa agggagcagt cgcaaacccc ttcgcgtcgt
     2401 ggtggactca ggaaacggcg tggccgggct ggtcgcgccc gcgctgcttc gcgccgtcgg
     2461 gtgcgacgtg atcgaactgt acagcgaacc ggacgcgcgg tttccccacc atcatcccga
     2521 tcccacggtg ctggacaacc tccgggatct gattgcggcg gtcggccgtc atcacgcgga
     2581 cctgggcatc gcgtacgacg gcgacgcgga ccggatcgga gtgatcgacg aacgcgggga
     2641 tgtgttgtgg ggtgaccggc tgctcgcgct gctcgcgggc ccggtcctgg ccgatcgccc
     2701 cggcgcgccg gtcatcgccg aggccaagtg ttcgcaggtg ctctatgatg agatcaccag
     2761 gcgcggcggc cgggccgtga tgtggaagac cgggcactcg ttgatcaagg cccgcatgaa
     2821 ggaactgtcc gcgccgttgg ccggagaaat gagcggccac ctgtttttcg cggaccggta
     2881 ctacgggttc gacgacgcca tgtacgcgtc gtgccgcgtg gtcgaattgc tcgtgcgtga
     2941 gtcccgacca ttgtccgcgc tcgcggccgc gttgcccgcg actgtggtca cccccgagat
     3001 ccgtgtcgac tgccccgacg agcacaagtt cggcgtggtg acccggctcc agacattttt
     3061 ccgggacgcg cgcgccaagt ccggcgagtt tccgctgccg atcctggaag tgatcgacgt
     3121 ggacggcgtg cgcgtgcgca cgccccacgg gtggggactg gtccgcgcct cgaataccca
     3181 gcccgtgttg gtgctccgat tcgagtcgga taccgaggcg cacctgaaag aaatccgatc
     3241 cgcaatcgag tcacgggtca aggagtgctg gttcgcctcc taatcctcgc ggcgttcttg
     3301 gccgtgggtt gccgcgcccc ggcgagttcc gacaacacat tccgcatggt gctgtccaac
     3361 gagccgccca cgctcgattg gtcgctcgcc accgacagcg tctcgatcac ggtcattgaa
     3421 aatctgatgg atgggctcac gcggttcgac caggacttgc ggcccgcccc tgcggtggcc
     3481 tcctcgtggg acgtgtcggc ggacgggagg cggtacctgt tccacctccg ccccgacgtc
     3541 aggtggtccg acggcgcgcc cgtgaccgcg gcggactttg agtacgcgtg gaagcgcctg
     3601 ctcgatccca agaccgcggc gcaatacgcg tattttctct atccggtggc caacgccaag
     3661 gcgtacaaca ccggcgcgtt gcccgacgcg tcgacggtcg gcgtccgggc gctctccgct
     3721 acggagctgg aagtcgtgct cgatcgcccc atcgcgtatt tcccgagcct taccaccttc
     3781 atggtgacgt ttccgcagcg acgggatctc attgaacggt acggggatcg ctggaccgat
     3841 ccgggacgtc tcgtgaccaa cgggccgttt cggctcgaat cgtggcggca cgaatatcgc
     3901 ctccagttgt cggccaatcc cacgtattac gggggcaagc ccgcgatcgg tcacgtggcc
     3961 atgtacgtgg tcaatgaacg gacgaccgaa ctgacgctct acgaaaccgg agacctcgat
     4021 tacgcgcaac tgccgcccga agcgatcccg gcgttcgaag gctcgcccga tcaccggcga
     4081 ttgcccattc ttcgcggata ctacgcgggc ttcaacgtga ccaagccgcc gtttaccgat
     4141 ccgcgcgtgc gccgggcgtt tgcgttggcg ctcgatcggg aggaattccc caagatcctt
     4201 cgcggcgggg agcgcgcggc gcgcagttgg gtcccgcccg gcatgttcgg gtacgagccc
     4261 gacatcgggt tgccgtacga ccccgagcgg gcgcggcggt tgttggccga ggcgggattc
     4321 cccggcggac gcgggttgcc gcccatcgag tacgtgttca acaccgggcc ggtgaacacg
     4381 ttgatcgcgg agaatctcca ggcgcaatgg aaacggaacc tgggcgtgga cgtgcggttg
     4441 gacaacgtgg agtggaaggt ctatctgaaa cgattggagc gggacgcgcc gggcatgttt
     4501 cgactgggat ggggcgcgga ctatccggac ccggataact ttctcgccct gttcaccaag
     4561 gactcgggca acaaccactc gcgctgggcc aacgcccggt acgacgcgct gatcgcggag
     4621 gcgtcaagcg aatccgatcc cgcccgccga ctggtcatgt atcgggaggc gcaacgcctg
     4681 ctgaccgaag aggacgtgcc gatcatcccc ttgttcaccc acgtgcagaa catgctggtc
     4741 aaaccgtggg tccgcggcct ggacgtcaac gccatggaga ttatgtcgct gcaaggcgtc
     4801 agcatcggag cccgtccatg aacctggccg cgccaaaggc gcgagtcgag aacgggccgt
     4861 tggaacccta caggtggcga agcaacggtc atctgcggcg ttgtcgctgc gcgtccgccc
     4921 cggccgcctc accgcctcgg cggcgctcgt aacatggcgc tccttcttcg tcgcgccact
     4981 cgccctcacg tacgacccag tacgctgcgg tgcgggcgct cgctccgcct tgcgacccac
     5041 cgccaaaaaa tcaaatgccg gaagtccccg aagggggcat ctgacgcgct cctgaacggg
     5101 ctccgaatcc ggcgcgactc ggacactgtt ctggatccaa gacaccgatg atccaggttg
     5161 tcgttcgccg cgtggtgtgg gggatcggcg tgctctggct cgtcgccacg gtgacgttcg
     5221 cgctgttgcg cgtggtcccg ggcggaccgt tcgactcgga aaaacggttg cctcccgaga
     5281 tcctggccaa cgtcgaggcc aagtaccact tggacgagcc ggtgtggcgt cagtacgccc
     5341 gatatctcgg cggcgtggcg cggggcgatc tgggcccgtc gttcaagtac ttcggccgcg
     5401 acgtggccgc gatcctcatg gagacctttc cggtgtcgtg cgcgctgggc ttcgcggcgc
     5461 tcggcatcgc ggttgtggcg ggcgtggcgt tcgggatcgc ggcgggaagc cgtccggacg
     5521 ggtggatgga tcgcggcggc atgctggcgg ccaccacggg cgtggcgctg ccgaatttcg
     5581 tgctgggcgt ggcgctcgtg ctgatcgtct cgcaccggct gcactggctg ccgcccgcgt
     5641 tgtgggaggg cccgcggtac gtgttgctcc ccgcgttgac cttggggctc gggcccgcgg
     5701 cgtacatcgc gcggctgacc cgcgccagtc tgctcgacgt ggcgcgccag gactacgtga
     5761 gaaccgcgcg tgccaagggg ttgagcgatc gcgcggtgct gatcgcgcat cagttgccca
     5821 acgcgctgat gccggtggtg acgttgttgg gaccgctcac cgccgcgctc atcacgggct
     5881 cgttcgtggt cgaatacctg ttcgcgatcc ccggcatggg gcgattcttc atcaccgcgg
     5941 tcacgaatcg cgactatcca ttgatcatgg gcgcgacctt gctctacgcg gcattgttgg
     6001 tggcggcgaa tctggccgcg gatctggtcg cgtcccgtct cgatccccgg attcgtctgg
     6061 aatgagggcg tccggatgac cgcggtgaat gtcatggtgt tgatcgtggt ggcgaccgcc
     6121 gccgtgttcg cgccggtgct cgccccgtac gcctacgaca cccaggacac ggccgcgatc
     6181 tacgcggagc cctcgtccgc ccactggatg ggcacggacc ggctgggccg cgatctgctg
     6241 tcgcgcgtga tctatggcac ccgcacctcg ttgctgatcg ggctcgcgtc cgcgttgatc
     6301 gcgctcacgc tgggcgcggc ctatggcggg atctcagggt acgcgggcgg gcggaccgat
     6361 caggcgctca tgcggagcct ggaggcgttg cacgcgttgc ccgatctcct ggtcatcatc
     6421 ctgctgagca cggtcttggg gcgcggcgtg ttcagcatga tcgcggcgat cagcctggtg
     6481 gggtggatga ccgtggcgcg attggttcgc ggcgagatgc tgcgcctgcg cgagcagccg
     6541 ttcgtagagg ccgcgagggc ctcgggcgcg ggccatgcgc ggatcctcct gcgccatctg
     6601 ttgcctcaaa ccatcggcct gctcatcgtc acgctcacgt tccgcatccc ggccggtatt
     6661 ctggcggaat ccacgctgag tttcgtgggc ctcggcatcg cgccgcccca agccagttgg
     6721 ggcagcctgg ccggggaagg ctggtccgcg atcaagttct acccccacgt gatcctgttt
     6781 cccagcctgg tgatcttcgt gacgatgctg gcgtttaact tgttgggaga ccggttgcag
     6841 gatatcttga acccggcgag aagccgccgt gtgggttagt aggacgacca gtaatcttgc
     6901 ttctattgtg acgggctcct aaggagtcag tttgagcacc tcgcccgcat aatccaccac
     6961 gtacaattcg cgctggtcgt cttcgccgaa cgcggaaatg ctgcggcccg tgtcgagcag
     7021 cttttgttgt tcgatcacgg tcgcgccgtc gtagcgcaat gcccagacgc ggccggtgcc
     7081 aaagtcgccg tacacgtagg cgccgcacaa tccgggaatg gcgcggccgc ggtacacgcg
     7141 gccgccgatc acggtggtgc cctcggtccg ctgataatcg aaaatcggtc gctcgaatcc
     7201 cgaggtcttg cacctgggat tcacgccggg cgtgcagacc gtgccctcca tcacgttcca
     7261 tccgtagttc ccgcccttgc ggatcacgtc gatttcttcc cgcgccttct gccccacgtc
     7321 gcccgcgaag agccagcccg tgaccggatc gaacgcaaac cgccaggggt tgcgcagccc
     7381 gtacgcccag atctccggcg ccgcgccttg tcgtttcatg aacgggttgt cgggcggaat
     7441 gccgtacgcc agttcgtcgc tgcggtggtc cacgtcgatg cgcaggatct tcccgagcag
     7501 ggtcgagagg ttctgcccgt tgccgagcgg atcgttcccc gaccctccgt ctcccagcgc
     7561 gatgtacaac atgccgtccg gcccgaacgc gaggtcgccg ccgttgtggt tcgagtaggg
     7621 ctgcggcacg gtcagcagaa tgcgttcaat gcgtgcgtcg gcctcgtccg cggcgtcgct
     7681 cgcgcggaat tccgaaatca cgctgtgcaa cccgccctta cgggacgtgt agttcacgaa
     7741 gaaccggccg ttctccgcaa aggtcgggtg gaacgcgagg cccaacagcc cgagttcgcc
     7801 gccggcgctc acgcgatcgc ggatatccag aaacgtcttg gatccggccc gacccggcca
     7861 gcggatcttg atggtcccgc tctgctcgac aataaagacg cggcccgagc cgtcacccgg
     7921 agaggacagg gccacgggtt tgttcaagcc cgtgaacgcg ggcgccagat gcaccggtgg
     7981 catcggctgg tcttggggag gcgtgggaca caacggcgcg gcgtgggccg gatcggaaag
     8041 ggtgatcgaa agcgtggtca gacacaggcc gaagccaaag gtaagcccca ggcagtttgt
     8101 caggcgcatg gcgattcctt cccaatcgag tggcaagacg ttttgtgccg cccatcatgg
     8161 cagagttttg cgacgaggtc tagtcccaat tcgttgcaaa tggcggatag acgcgatttt
     8221 ctgaggatca gacttggcgg gccgccggcc gggagtccga cctgtccggc cgttacttcg
     8281 gcagcggcgg cgggatcacg acgatcgggt cgctgctcag ggagcggatg aaggcgagta
     8341 gatccgcctt gtcttgttcg gtgagcaggt cgagcggggt gatcagcggg tggcgatggg
     8401 ccgggtgtcc ttcggccttc acgtaaaagt ccaacacgtc ttccagcgtg gcgaacatgc
     8461 cgttgtgcat gtagggcgcg ctgtccgcga cctggcgcaa tgacggcgtg cggaacgttt
     8521 ccatatcgtc gtcgttcaac gtgacgttgt aacggcccgg gtcatccggg acgccttcgc
     8581 gtttgggcag tcccaaagca tggaactggt tatccgtgaa attggggccg ttgtggcacg
     8641 tcacgcattt ggctttctcg tcgaacacgc gcaccccgcg tttggctgat tccgaaaggg
     8701 ccgcgtcatc gcccgccatg aaacggtccc acggagaatt gttcgagatg atcgtgcgtt
     8761 cgaacgcggc cagggcttgg gcaatgccgt ccgcgctggg gccggttccg aagacggcgc
     8821 gaaacgcctt cacgtagccg ggtaccgcgt tgagttcggc gacgagctgc gagaggtcct
     8881 gatccatctc gatcggattc tgaatcggtt ccagagcctg ttgttcgagc gaccccacgc
     8941 gtccgtccca gaagaactgc gtccggtagg ccacgttgta gagcgacggg acgttgcgag
     9001 cgcccagttg tcctctgacc cccgtggcca cgggttggcc gttggaaaat cccagcgctg
     9061 gcacgtgaca cgacg
//
LOCUS       DBNGTT010000297         4785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3130704, whole genome shotgun sequence.
ACCESSION   DBNGTT010000297 DBNGTT010000000
VERSION     DBNGTT010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3130704"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..542
                     /locus_tag="AC16BC_06635"
     CDS             75..542
                     /locus_tag="AC16BC_06635"
                     /inference="COORDINATES: protein motif:HMM:NF021425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HIR3241333.1"
                     /translation="MAKRASSEAFLKFIIIEKAFLGVLFVSLSIGVLGLIDKDLVVLG
                     RQLVAMFNLDTDNAYITLALNKLGLVDNKVILGLSIGGFTYAALNLVEAYGLHKRLRW
                     AEWLTVLATGILIPFEVYELWHRFTPVRVGVLVLNVAIVIYLAKHKELFPRWF"
     gene            complement(496..1248)
                     /locus_tag="AC16BC_06640"
     CDS             complement(496..1248)
                     /locus_tag="AC16BC_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241334.1"
                     /translation="MRALGSALYAALRGGGAVIEGAEFLIAAARLSPRRIGHLLAAAA
                     CVLIATKLFSGIATRLPTVPEISFPWYLVLVEWGWSNLSSGLAAAATAWGAETLYLGW
                     VSVRDVVDTVVSRRGGMAGRRAVVSARRAAIACLVVGGFGCVGVAYVPIVGPLAALAA
                     ACPVLGAGFAAACLAQRGWPPDEITRLVRRRVVLLGGLGGGVLVLLTIPLVNLVVLPC
                     AAAGTVCLLLREERADSVKTSAGTTPCVSPSK"
     gene            complement(1258..1914)
                     /locus_tag="AC16BC_06645"
     CDS             complement(1258..1914)
                     /locus_tag="AC16BC_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR3241335.1"
                     /translation="MTNRHIHEVLAELREAVRQWPTPVVGVVASETRDPFRVLISCLL
                     SLRTKDATTAAAAARLFALAATPDALRRLAPAAVEQAIYPVAFFRTKTRNLQALCDHL
                     LARFHGRVPDTIDDLLSLPGVGRKTANLVVTLGFGKPGICVDTHVHRISNRWGYVKTD
                     TPDETERALRRRLPRSYWIEFNDVLVRYGQHRCTPTSPFCSRCTISRWCRQVAVSRRR
                     "
     gene            complement(1911..2795)
                     /locus_tag="AC16BC_06650"
     CDS             complement(1911..2795)
                     /locus_tag="AC16BC_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR3241336.1"
                     /translation="MDIGFVQNSTIFGRVKTNLDRISDLVASVRADVLVLPELFATGY
                     QFTSQREAERLSEPVPDGVTTKRLITLAKRRKCVMVAGLAERKGRSLYNSAVVVGPNG
                     LIGLYRKSHLFYEETLWFTPGNTGFRVFQAGGAVLGVMICFDWFFPESTRLLSLAGAD
                     VICHPSNLVLPHAPNAMKTRCLENRVFAVTADRVGFEERGERERLTFIGSSQVVSPQG
                     EVLVRASTDREEVHVVTIDPKDARRKTINRYNHLFADRRPELYSGLATPLAEPLPQGP
                     TPSVRTRRVAKRPRPSGR"
     gene            3059..3514
                     /locus_tag="AC16BC_06655"
     CDS             3059..3514
                     /locus_tag="AC16BC_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR3241337.1"
                     /translation="MLIQMRVRGLMLDPPNNYVVILRGEEGQEVLPIWIGKAEANSIS
                     LALENVDLPRPLTHDLMKNALDALEAKVISVVITELKDSTYFAKLHLLHHDAEITVDT
                     RPSDAIALALRTDSPIFVAQEVLQRQLDDEDIAKWLENLRPEDYGKADA"
     gene            3579..4166
                     /locus_tag="AC16BC_06660"
     CDS             3579..4166
                     /locus_tag="AC16BC_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HIR3241338.1"
                     /translation="MQVYLSENVFMGMMLSTVEVYKNECFGLLLGYRLPDRYVVEHAI
                     PYQSVRRGRNFAELRSDKWRVIQEVLKWFPRLDVLGDYHSHTMYGNVRAEVALSRDDI
                     DYMEPNDLQIVIAVNENRRTRGWTVNTDRTVSGSLDRYHFKIAAYYDANRVGGRAISR
                     KVASARFRTRPGPMAPRLELATILCPFALSARRAF"
     gene            4181..4630
                     /locus_tag="AC16BC_06665"
     CDS             4181..4630
                     /locus_tag="AC16BC_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR3241339.1"
                     /translation="MDVEVSVRGVFPDPATEAQIVVLEEEGTSRTLPIWVGMVEGNAI
                     RLAVERGSSPRPLTHELLQQLLDKLHAQVTRIVVRDVRDNTFYASIVLDVQGREVVLD
                     ARPSDAIALALRVGSQIYARRELMAGSESDRVREWLEDLKPTDFEAP"
BASE COUNT          968 a         1423 c         1535 g          859 t
ORIGIN      
        1 ttgcggcgac gccgggcatc tgataggatc gcgactttgc cgcgcaaact gcggatcggc
       61 cttgatcgac cggtatggcg aaacgagcat cctcagaggc gtttcttaaa tttatcatca
      121 tcgagaaggc atttctcggg gtgttgttcg tcagtctgtc gatcggggtg ctgggtttga
      181 tcgacaaaga tttagtggtg ctcggcaggc aattggtcgc catgttcaat ctcgacaccg
      241 acaacgccta tatcaccctc gcgctgaata aactcggact cgtggacaac aaagtcatcc
      301 tcggtctgtc gatcggggga ttcacgtacg ccgccctcaa tctggtggaa gcgtacggac
      361 tccataaacg gctccggtgg gcggaatggt tgacggtgct tgccaccggg attctcatcc
      421 cgttcgaagt gtacgagctc tggcatcggt ttaccccggt tcgggtcggc gtgctggtcc
      481 tgaacgtggc gatcgtcatt tacttggcga aacacaagga gttgttcccg cgctggtttt
      541 gacggagtcg gcgcgttctt cgcggagcag caggcagacc gtgcctgctg ccgcgcacgg
      601 cagaaccacc aggttcacca gcgggatcgt gagcaagacg agcacccctc cgcccaggcc
      661 gccgagcagc accaccctcc ggcggactaa tcttgtgatc tcgtcggggg gccatccgcg
      721 ttgggcaagg caagccgccg cgaatccggc gcccagcacc gggcaggccg ctgcgagggc
      781 cgcaagcggc cccacgattg gcacgtaggc caccccgacg cagccgaacc ctccaaccac
      841 gaggcacgcg atcgcggctc ggcgcgcact gacgaccgcg cgccgccccg ccataccgcc
      901 ccgacggctc accacggtgt ccacgacgtc gcgaacggac acccatccga gatagagcgt
      961 ttccgcgccc cacgccgtcg cggccgccgc aagcccggac gaaaggttcg accagcccca
     1021 ctccaccagc acgaggtacc acggaaagga gatctccggc actgtcggga gccgcgtggc
     1081 aatcccggaa aagagcttgg tcgcgatcag aacacaggcc gcagcggcca ggaggtgccc
     1141 gatccgtcgc ggagaaagac gcgccgcggc gatcagaaac tcggcgccct cgatgaccgc
     1201 gccgccgcct cgcagcgcgg catagagtgc ggagccaagc gcgcgcatcg gacgcggtta
     1261 ccggcggcgt gaaaccgcga cttgccgaca ccagcgggaa atcgtgcagc ggctgcagaa
     1321 cggcgacgtc ggcgtacagc ggtgttgccc gtagcgcacg agcacgtcgt tgaattctat
     1381 ccagtacgat cgtggcagtc gacggcggag cgcccgctcg gtttcgtccg gcgtgtcggt
     1441 cttgacgtac ccccagcgat tcgagatgcg gtggacgtgc gtgtccacac agatgccggg
     1501 ttttccgaac cccaaggtga cgaccagatt cgcggttttc cgtcccacac cagggagcga
     1561 gagcagatca tcgatggtat cgggcacgcg tccgtggaac cgagcgagaa ggtgatcgca
     1621 gagcgcctga aggttcctcg ttttggtgcg gaaaaacgcc accggataaa tcgcctgttc
     1681 gaccgcggcc ggagccaagc gccgcagggc gtcaggggtc gcggcgagcg caaacagccg
     1741 agcggccgcc gcggcggtcg tggcgtcttt ggtccgaaga ctgaggagac aggagatcag
     1801 gacgcggaag ggatcgcgcg tttccgaagc caccacgccg accacagggg tcggccactg
     1861 gcgcaccgct tcgcgcaact ccgcgagcac ttcatggata tgccgattgg tcatcgcccg
     1921 gacgggcggg ggcgttttgc aacccgccgc gtcctgaccg agggggtcgg gccctgggga
     1981 aggggttcag ccaacggcgt cgcgaggccg gagtacaact cgggtctgcg atcggcaaag
     2041 aggtggttat agcggttgat ggtctttcgg cgagcatctt tggggtcgat cgtgaccacg
     2101 tgcacctctt cacgatcggt ggatgcgcgg accagcactt cgccttgggg gctgacgacc
     2161 tggctggacc cgataaacgt gagccgttcc cgttcgccgc gttcctcgaa tcccacgcga
     2221 tccgccgtga cggcgaacac gcggttttcc aagcaccggg tcttcatcgc gttcggggcg
     2281 tgcgggagga ccaagttcga cgggtggcag atcacgtcgg cgccggccag cgaaagcagc
     2341 cgcgtcgatt ccggaaagaa ccagtcaaaa cagatcatga ccccgaggac ggcgcctccg
     2401 gcctgaaaaa cgcgaaaccc cgtgttgcct ggcgtgaacc ataaggtttc ttcgtaaaag
     2461 agatggcttt tccggtagag cccgatcaat ccattggggc cgaccaccac ggccgagtta
     2521 tagagcgagc ggccctttcg ctcggcaagg ccggcaacca tgacgcactt acggcgcttc
     2581 gccagggtga tcagacgctt cgtggttacg ccgtctggaa cgggctccga gagtcgttcc
     2641 gcctcgcgtt gcgacgtaaa ctgatacccc gtggcaaaga gctcagggag gacgaggaca
     2701 tccgcgcgta cggaggcgac gagatcggaa atccgatcaa gattggtctt cacccgccca
     2761 aagatcgtgg agttttggac aaacccgatg tccacgtgat tcctctcgag gttggtgcgg
     2821 aacaataaca tagccgcgag gctgcgtaca aatgcgaggc atccaacaca cggatcgaaa
     2881 tgggcgggag aagatcattg atccgagtaa cgtccaaaac gcgatgaatc gtcaataaaa
     2941 tcaatgtagt tgacaacatt tagaggctgg tgttacaaca cgttcatatc actttcatca
     3001 ttattcaaac acttgcgcct ggagagaact ggggcgtgag aaacggtctg aggccagtat
     3061 gctcatccag atgcgtgttc ggggacttat gctcgatcct ccgaataact acgtggtgat
     3121 tcttcgcggg gaggaagggc aggaggttct gccgatctgg atcgggaagg ccgaagccaa
     3181 ctccatcagt ttggcgctcg aaaatgtcga cctgccgcgt ccgttgaccc acgatttaat
     3241 gaagaatgcg ctggacgcac tggaagcgaa agtcatcagc gtcgtgatta ccgagttgaa
     3301 ggacagcacc tatttcgcga agctgcacct tctgcatcat gacgcggaaa tcaccgtgga
     3361 cacgcgcccg agcgacgcga tcgccctggc gttgaggacc gacagcccga tttttgtcgc
     3421 ccaggaagtg ctgcagcggc aactggacga tgaagacatc gccaagtggc tggagaacct
     3481 ccggcccgag gattatggga aggccgatgc gtagcgtcac gatcccccgg aagcgagcga
     3541 aacacgcggg cgagaggtcc caaacttcag gacaccccat gcaggtctac ctgagcgaaa
     3601 acgtgttcat ggggatgatg ctgtcgacgg tcgaggtgta taagaacgaa tgcttcggct
     3661 tgttattggg ctatcgcttg ccggatcgct acgtggtcga acacgcgatt ccctaccaaa
     3721 gcgtgaggcg cggccggaac tttgccgaac tgcgaagcga caagtggcgc gtgattcagg
     3781 aggtgctcaa gtggttcccc cgactcgacg tgctcgggga ttatcactcg cacacgatgt
     3841 acggcaatgt gcgggcggaa gttgcgctga gccgcgatga catcgactat atggaaccca
     3901 acgacctgca gatcgtgatt gcggtcaacg agaatcggcg cacgaggggt tggaccgtca
     3961 ataccgaccg gaccgtgtcc ggctcgttgg atcggtacca tttcaagatc gccgcgtatt
     4021 acgacgccaa tcgcgtgggt gggcgcgcga tttcgcgaaa ggttgcgagc gctagattcc
     4081 ggacgcggcc gggcccgatg gctccgaggc tggagcttgc gaccattctg tgcccattcg
     4141 cgctgagcgc gcggcgggcg ttctgagtcg ggaggatttc gtggacgtcg aggtgtcggt
     4201 gcgtggcgtg tttccagatc ccgccacgga ggctcagatc gtggtgcttg aggaagaagg
     4261 gacctcgcgg accttgccga tctgggtggg gatggtcgag ggcaacgcca tccgcttggc
     4321 cgttgagcgc ggctcctcgc cccgccccct cacgcacgag ctcctccagc agttactgga
     4381 caagctccat gcccaggtca cccggatcgt ggtgcgcgat gtgcgagaca acacgtttta
     4441 tgcctcgatc gtgcttgacg tgcagggccg tgaagtggtc ttggatgcgc gcccaagcga
     4501 cgcgatcgcc ctggcccttc gagtcggctc gcagatctac gccagacggg agctgatggc
     4561 ggggtcagag tccgatcgcg tccgcgagtg gctggaagac ctcaaaccca cggacttcga
     4621 ggccccgtaa ggccggaaca agtcgggatc agagcgagag ctctgcggtc gccagatcga
     4681 cctcgacggc aggagactcg tcctgatcgc ggtccttatg cagtttgacg gacacgattt
     4741 tcgacacccc ggcctcttcc atcgtgactc cgtacaggac gtcgg
//
LOCUS       DBNGTT010000298         6753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2619786, whole genome shotgun sequence.
ACCESSION   DBNGTT010000298 DBNGTT010000000
VERSION     DBNGTT010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2619786"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="AC16BC_06670"
     CDS             <1..752
                     /locus_tag="AC16BC_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR3241340.1"
                     /translation="PSDWPLEALKAQAVLARTYVLYQKAERKTSDFDVDATVQSQVYG
                     GVPSEDGRTSEAVAATAGRVVLFDGRLAFTPYHSTSSGQTEDAMEVWGVDQPYLKGVD
                     CSFDSASPAARWTRRIPLEEIEAALGQAGYRVGPIASITPMSRNRSGRVSSVRIVHAG
                     GALVLKGEGLRRIIGYQRLPSMRFTLTEFAVNPDTRRVEAWFEGGGWGHGVGLCQWGM
                     KALAERGWTAERIITYYYPGTQVDVAPRTAR"
     gene            727..1803
                     /gene="queA"
                     /locus_tag="AC16BC_06675"
     CDS             727..1803
                     /gene="queA"
                     /locus_tag="AC16BC_06675"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR3241341.1"
                     /translation="MSPRELLADSAAVETDDERLDAFDYPDAWAIIADRPAPVRDASR
                     LLVYDRRTGRVDHRRFLDLPEYVLSGDIVVVNDSRVIPARLAGVKVPSGGHIELLLTR
                     REEPGVWRVLVKGKVRPGQRIAFDAGGEGEFLDPDDGRARLRLTPDDPAWLERVGEVP
                     LPPYIAKVRGADAAWAEDRSRYQTVYARADGSVAAPTAGLHFTGAVFDGLARRGASVA
                     AVTLHVGPGTFEPVRAERIADHRMAAEWGEVSPDAARVITRAREAGGRVIAVGTTATR
                     LLETAGAGGALRPYNGETDVFIRPGHRWRVVDALLTNFHLPRSTPLLLAAALCGRAAL
                     LELYREAAAEGYRFYSYGDAMLIL"
     gene            1803..2471
                     /locus_tag="AC16BC_06680"
     CDS             1803..2471
                     /locus_tag="AC16BC_06680"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3241342.1"
                     /translation="MAAAQAKSRTQSDRASGPSLKPYLAIVALLAVVGVASFSLVERW
                     LSEPDPDSSTAEPPIADGGRVVIGRSASPAAPQQRPAAGSRSGEAPYKPQEFTFYKSL
                     GNAPETTPKLEPTRPSGAPASRSEPAIKPGERARATARKSYTVQIGSFQDRKSADRLS
                     AKVRRYQYTVSVNRVVLPDSGVRYRVRVGSFATREDALKVAAMLKKKERVEPFVALVA
                     PSSG"
     gene            complement(2673..2957)
                     /locus_tag="AC16BC_06685"
     CDS             complement(2673..2957)
                     /locus_tag="AC16BC_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241343.1"
                     /translation="MSDVQMQGKSLAAIARDIAEGMISLTPLLLKHLDEGGVRALHHQ
                     LRKHQTEVRGSKFPAHDMQGIRTRNLRLQRLHQAITVLEHAARQLRIRIA"
     gene            3008..5029
                     /gene="yoaA"
                     /locus_tag="AC16BC_06690"
     CDS             3008..5029
                     /gene="yoaA"
                     /locus_tag="AC16BC_06690"
                     /inference="COORDINATES: protein motif:HMM:NF053712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase YoaA"
                     /protein_id="HIR3241344.1"
                     /translation="MSTPNTDHTEAAVERVWSLDGPLARTLSGFEARPQQVAMARAVA
                     DALANDRTLLVEAGTGTGKTLAYLVPALLSGKKVVVSTGTRALQDQLARHDVPLVERV
                     LGRSVDWCVLKGRDNYLCLYRLEDGDAAPRLGASTSGAPFRDPALDAIRAWAAETETG
                     DRAELNWLGEDDPLWTRVSVTAEQCLGASCPRYEPCFLTQVKRRAARADLVIVNHALF
                     FADLAVGPHASGSIVPRHAAVVFDEAHALEEVATAHWGVSVSRHRVRDLLADLRREPL
                     PDDGLSGAAVFHGALDRVHAAADRAFDALRAATGADAGTRARWTPDGAERAAGAGLDL
                     LARLGEFQDGLDGLSNRSEGWARLAERVGALARDARFLWRAEDPTFVFWIEVNGAAVR
                     LSASPLDVSEILRETLFSSGMPCVLTSATLTAGGSFEYLRSRLGIDEAGELSLASPFD
                     FRRQALVYVPDAFPLPRDPEFLDAVADEVVRLVAVSRGRAFVLFTSRRALERVFDACR
                     RRIGYPALRQGEAPRMALIERFKAEPSVLFATMGFWQGIDVPGAALSFVVLDKLPFAP
                     PDDPLVQARTGALKAGGLDPFTRYHLPTAAIWLKQGFGRLIRTQRDRGVIAILDRRLL
                     THGYGRFFLDSLPPAPVTTSFRDVERFFATANSGDASEGLAVPPQSEYS"
     gene            5064..5489
                     /locus_tag="AC16BC_06695"
     CDS             5064..5489
                     /locus_tag="AC16BC_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241345.1"
                     /translation="MRIPLKWITGLVVIGTVGCVIVPDGPPGHARRGAVVMAPPPVVI
                     AARPRMVFVTEFGVSFAPELDFDVYEVGGVWYSFRSDVWYHADAYDGPWVVVKRGHLP
                     KGLVKVKPGQVRKFYFENEGKKNRDHGERGRGRDRREDD"
     gene            complement(5547..6500)
                     /locus_tag="AC16BC_06700"
     CDS             complement(5547..6500)
                     /locus_tag="AC16BC_06700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3241346.1"
                     /translation="MTYLILGYCRFKIVAGLTLIALFLGASAARAAALQAVAPDVYVF
                     PGADGDANAGVVVGDQGVVVIDSSPTPETARALKAEIKRLTPKPILYVVLTHFHGDHT
                     FSVHELAPTRGIISHVYTKDYLAEHGADHLKQCRAMLGEAKTQGAKVTLPTITFPDMY
                     SIPMGKQHILLFYQGRGHTRGDIVAYLPTAHVLFTGDLAYAGRVPWMGDAYVKEWQQT
                     LDRLAVLDVTAIVPGHGKWGGMPVLTTFREYLTDLYSAVFVSWMIKRPIEAAKKEVQL
                     PKYESWLKYKEWLPANVEKVYQELVAESEGAPSFETMPPAQ"
BASE COUNT         1022 a         2112 c         2396 g         1223 t
ORIGIN      
        1 tgccgtccga ttggccgttg gaggcgctca aggcccaggc cgtcctggcg cggacatacg
       61 tgctctatca gaaagccgaa cgcaaaacga gcgactttga cgtggacgcc acggtccaga
      121 gccaggtgta cggcggggtg ccgagcgagg atggccgcac gtcggaggcg gtcgcggcca
      181 cggccggccg cgtggtgctg ttcgacgggc gtctggcgtt caccccgtac cactccacgt
      241 cctcggggca gaccgaggac gcgatggagg tgtggggcgt ggaccagccg tacctcaagg
      301 gcgtggattg ctccttcgat tccgcctccc ccgccgcgcg gtggacgcgc cggattccgt
      361 tggaggagat cgaggccgca ttgggccaag cgggataccg cgtcgggccg atcgcgtcga
      421 tcacgccgat gtcccgaaac cgctccggtc gcgtctcgtc ggttcgcatc gtgcatgcgg
      481 gcggcgcgct cgtcttgaag ggagaagggc tgcgccgtat aatcggctac caacgcttgc
      541 ccagtatgcg atttacgctc acggaattcg cggtcaaccc ggacacgcgg cgcgtggagg
      601 cctggttcga gggcggcggc tggggccacg gcgtcggcct ctgccagtgg gggatgaagg
      661 ccctcgcgga gcggggctgg accgcggaac gaatcatcac gtactactac cctgggaccc
      721 aagtcgatgt cgccccgcga actgctcgct gattccgcgg ccgtggaaac ggacgacgag
      781 cgtctcgatg cattcgatta cccggacgcg tgggcgatca tcgcggaccg gcctgctccg
      841 gtccgcgatg cgtcgcgcct gctggtctac gatcggcgca cgggccgcgt cgaccatcgg
      901 cggttcctcg acctgcccga gtatgtcctg tccggcgaca tcgtggtcgt caacgattcg
      961 cgcgtgattc ccgcccggct ggcgggcgtg aaggtgccca gcggcgggca catcgaactc
     1021 ctcttgaccc ggcgcgagga gccaggcgtg tggcgcgttc tcgtgaaagg caaggtccgt
     1081 cccgggcaac ggatcgcgtt cgatgcgggc ggcgaggggg agttcctgga tcccgacgac
     1141 gggagggctc ggctgaggct gaccccggac gacccggcgt ggttggaacg cgtcggcgag
     1201 gttccgctcc cgccgtacat cgcgaaagtc cgtggagccg acgccgcctg ggcggaagat
     1261 cgctcgcggt atcagaccgt gtacgcgcgg gcggacggct ccgtggccgc tcccaccgcg
     1321 ggtcttcact tcaccggcgc ggttttcgac gggctcgcgc gacgcggcgc gtccgtggcg
     1381 gccgtgacgc tccatgtggg gccggggacc ttcgagccgg tgcgcgccga gcggatcgcc
     1441 gaccaccgga tggccgcgga gtggggggaa gtctcgcccg acgcggcgcg cgtgatcacc
     1501 cgcgcccgcg aggccggcgg gcgggtgatc gcggtcggca ccaccgcgac gcgcctgttg
     1561 gaaaccgccg gggcgggcgg agccctccgg ccctataacg gcgagacgga tgtcttcatc
     1621 cgtcccggcc atcgatggcg ggtggtggac gcgctgttga cgaactttca tctgccgcgc
     1681 tcgaccccac tgctgctcgc ggccgccctg tgcgggcgcg cggcgttgtt ggagttgtac
     1741 cgggaggccg cggccgaggg ctaccggttt tacagctacg gcgacgcgat gctgatcctc
     1801 tgatggccgc ggcccaggcg aaatcacgga ctcaatccga ccgggcctcg ggcccgtcgc
     1861 tcaaaccgta tctggcgatc gtggcgttgc tggccgtggt cggcgtcgcg agtttttcat
     1921 tggtggagcg gtggttgtcg gagccggatc cggattcttc gaccgccgaa cccccgattg
     1981 ccgacggggg ccgcgtggtg atcgggcgat cggcctcccc cgcagcgccc cagcagcggc
     2041 cggccgccgg gtcgcggtcc ggtgaggcgc cctacaagcc gcaggagttc acgttctaca
     2101 agtcgttggg gaacgccccc gagacgactc cgaagctcga accaacgcga ccgtccggcg
     2161 ctccggcctc gcgtagcgag ccggcgatca agcccggcga gcgcgctcgg gccacggctc
     2221 gcaaatcata cacggtgcag atcggttcgt ttcaggaccg aaagtcagcc gatcgactct
     2281 ccgccaaggt gcgtcggtat caatacaccg tatcggtgaa ccgcgtggtg ttgccggatt
     2341 ccggcgtgcg ctaccgtgta cgtgtcgggt ctttcgccac gcgcgaggat gcgctcaaag
     2401 tcgccgcgat gctcaagaag aaagagcggg tggagccgtt tgtggcgctg gtcgcgccct
     2461 cctccgggtg aggaggccgt ccaccgtatt aggtgtggtg agcctgttta gcagtggcca
     2521 gatgtcccct tcggggactt caggcatttc ttcttttgaa ggggggtcgc aaggcgccgc
     2581 gagcaccgcc cgcaggggtt cggcatgccg aacccctaca ccaatgggga tgcacaacgg
     2641 caacgccgcg gcaggccgct gatgaacagg cgctacgcga tgcgaattct taattgtctc
     2701 gccgcgtgtt ccaggacggt gatggcttga tggagccgct gaagtcgaag gttgcgggtt
     2761 cggatgccct gcatgtcatg ggcgggaaac ttggagccgc ggacttcggt ttggtgtttt
     2821 cggagttggt ggtgcaatgc gcgcaccccg ccctcatcca aatgtttgag caggagcgga
     2881 gtcagggaaa tcatcccttc tgcgatatcg cgcgcgatgg ccgcgaggct cttcccctgc
     2941 atctgcacgt ctgacatggc cgctgcctcc gatacgcaac ggcctaaaga tatcgtgcgc
     3001 ctcccccgtg tcaacgccga acactgacca cacggaagcc gcggtcgagc gcgtgtggag
     3061 cctcgacggc ccgctagcgc gcacgttgtc gggctttgag gcgcggcctc aacaagtcgc
     3121 catggcccgc gccgtggccg acgcgctggc caacgaccgg actcttctgg tcgaggccgg
     3181 gactggtacg ggcaagacgt tggcctatct ggttccggcg ttgctgagcg gaaaaaaagt
     3241 cgtcgtgtcc accggcacgc gggcgttgca ggatcaactc gcccgccacg acgtgccgct
     3301 ggtcgagcgc gtgctcggtc gctcggtgga ctggtgcgtg ctgaaggggc gcgacaacta
     3361 tctctgcttg taccggcttg aggacggtga cgccgcgccc cgactggggg cgtcgacgag
     3421 tggggcaccc tttcgggatc cggcgctcga cgcgatacgc gcttgggccg cggagaccga
     3481 gaccggggat cgcgcggagt tgaactggct cggggaagac gacccgctgt ggacgcgggt
     3541 gtcggtgacg gccgagcagt gcctgggagc gagttgcccg cgctacgaac cgtgttttct
     3601 cactcaggtc aaacgccgcg ccgcgcgtgc tgatctcgtc atcgtgaacc acgcgctgtt
     3661 ttttgcggat cttgcggtcg gtccccacgc ctcggggtcc atcgtgccgc gccacgcggc
     3721 ggtggtgttc gacgaggcgc acgcgctgga ggaggtggcg accgctcatt ggggcgtgag
     3781 cgtgtcgcgg caccgcgtgc gcgatttgct cgcggacctg aggcgcgagc cgttgccgga
     3841 cgacggcctg tcgggcgccg cggtgttcca cggggcgctg gatcgggtcc atgccgcggc
     3901 cgaccgcgcg ttcgacgcgt tgcgcgcggc caccggcgcg gacgcgggaa ctcgcgcgcg
     3961 gtggaccccg gacggcgcgg aacgcgcggc cggggcggga ctcgatctgt tggcccggct
     4021 gggtgagttc caggacgggc tcgacggcct gagcaatcgc agcgagggat gggcccggct
     4081 cgcggagcgc gtgggcgcgt tggcgcgcga cgcgcggttt ctctggcgcg cggaggaccc
     4141 gacgttcgtc ttctggatcg aggtcaacgg ggcggcggtt cgtctgtccg catcgccgtt
     4201 ggacgtgtcg gagattttgc gggagacgct cttctcctcg ggcatgccgt gcgtgctgac
     4261 ttccgcgacg ctcaccgccg ggggatcgtt tgaatatctg cggagccggt tgggtatcga
     4321 cgaggccggc gaactctcgc ttgcttcgcc gtttgatttt cgtcggcagg ccctggtgta
     4381 cgtgcccgac gcgtttccgc tcccgcgcga tcccgaattc ctcgacgcgg tcgcggacga
     4441 agtcgtcagg ttggtggcgg tgagtcgcgg acgcgcgttc gtcctgttca ccagccggcg
     4501 ggcgctggag cgcgtctttg acgcgtgccg ccggcggatc ggctatcccg cgctccggca
     4561 gggcgaggcc ccgcgcatgg ccttgatcga gcggttcaag gccgaaccgt cggttctgtt
     4621 tgccaccatg ggattctggc aagggatcga cgtgcctggg gcggcgctgt cattcgtggt
     4681 cctcgacaag ctgccgtttg ccccgcccga cgatcccctg gtccaggcca ggaccggcgc
     4741 gctcaaggcc ggggggctgg accccttcac gcgataccat ttgcccaccg cggcgatctg
     4801 gctcaaacaa ggcttcggcc ggttgattcg cacgcagcga gaccgcggcg tgatcgcgat
     4861 tttggaccgg cgactgctca cccacggtta cgggcggttc ttcctcgatt cattgccgcc
     4921 ggcgccagtg accacgtcat tccgcgacgt ggaacggttc ttcgcgacgg ccaactccgg
     4981 cgacgcctca gagggcctgg cggttccgcc gcagtcggag tattcttaac cccagcgatt
     5041 cactgacgga caaggagaga ccgatgcgga ttccactgaa atggatcact ggactcgtcg
     5101 tgattggcac cgtcgggtgc gtcatcgtac ccgacgggcc gcccggccat gcgaggcgcg
     5161 gcgccgtggt gatggccccg ccgccggttg tgatcgcggc ccgtccgcgg atggtgtttg
     5221 tgaccgagtt cggggtttca tttgcgcctg aactggattt tgacgtctac gaggtcgggg
     5281 gcgtgtggta ctcgtttcgc tcggacgtgt ggtaccacgc ggacgcgtac gatggtccgt
     5341 gggtggtggt gaaacgcggc caccttccca aaggactcgt caaggtcaag ccgggccagg
     5401 tccggaagtt ctatttcgaa aacgagggga agaagaaccg tgaccacggg gagcgtgggc
     5461 gcggccggga tcggcgcgag gacgattgaa ttggatcgat tgaagacaga tcgagcggtc
     5521 gtcccggcgg gacgccgctc acgccgttac tgcgccggcg gcatggtttc gaagctgggc
     5581 gcgccttcgg attccgcgac caactcctgg tagacctttt ccacgttcgc ggggagccac
     5641 tccttgtatt tgagccagct ctcgtatttg gggagttgga cttccttctt cgcggcctcg
     5701 atcggccgtt tgatcatcca ggagacgaac acggcggagt agagatcggt gagatattcg
     5761 cggaacgtgg tgagcaccgg cattccgccc cacttgccgt gaccggggac aatggccgtc
     5821 acgtccagga ccgcgaggcg gtcgagcgtc tgctgccact ccttgacgta ggcgtcgccc
     5881 atccatggca cccgtccggc gtacgcgagg tcacccgtga acaggacgtg cgcggtcgga
     5941 agatacgcca caatgtctcc gcgggtgtgg ccgcggccct ggtagaacag caggatgtgc
     6001 tgtttcccca tggggatgct gtacatatcg ggaaaggtga tggtgggtaa cgtgactttg
     6061 gcgccctgag tcttggcctc acccaacatg gcccggcatt gcttgagatg gtcggccccg
     6121 tgttcggcca aataatcctt ggtgtagacg tgggaaatga tgcctcgggt cggcgccagt
     6181 tcgtggaccg agaacgtgtg gtccccgtga aaatgagtca ggacgacgta caagatcggc
     6241 ttgggggtca ggcgtttgat ctcggcttta agcgcccgcg cggtttcagg cgtggggctg
     6301 gagtcgatga ccacgacgcc ctgatccccg accacgacgc cggcgttggc gtcgccgtcc
     6361 gctccgggaa acacgtacac gtcgggcgcc acggcttgca gcgcggcggc ccgcgcggcg
     6421 gacgcgccga ggaacaacgc gatgagcgtc aaccccgcga cgatcttgaa tcgacaatat
     6481 cccaaaataa gataagtcat gagccggccg gagcctaaca cgcagcgcgt ggggcggtca
     6541 agtcccgcgg tcgcgtgcct tgcaatttac ccatgttctt tgtattcaga acgccgtttt
     6601 gtgccggcgt gtcatagggc gagacgaatc cccgagcacc cggtagccgt ctcatttcga
     6661 aacagggagg cgtgtcatat ggcgtggtca cgtgcaggat ggtcggccgt cgcaacctcg
     6721 gcggtgttcg cagtagtgct ctccggatgt ccc
//
LOCUS       DBNGTT010000299         5643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_832242, whole genome shotgun sequence.
ACCESSION   DBNGTT010000299 DBNGTT010000000
VERSION     DBNGTT010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_832242"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="AC16BC_06705"
     CDS             <1..852
                     /locus_tag="AC16BC_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3241347.1"
                     /translation="DGRLADLTTAFPPPADKPELSPRYGIIIGKELALRLGVFTGDTL
                     NVVSPQGTMGPFGVVPKLRPYQVVGIFESGMYDYDSSFAYISLAQAQDFFQLGDAVTG
                     IEIKVDEIFRAGDVASAADAALGGRYLVRDWKEMNRNLFSALKLEKIIMFIILVLIIL
                     VAAFNIIGTLTMIVIEKSREIAILKAMGATRRAVMGIFMLDGLIIGGTGVAIGIPLGY
                     AVCWAIEHFYTLPADVYYISHIPVRIYAFDVALVAGSALMITFLATMYPSWQAGRLAP
                     VEALRYE"
     gene            845..1519
                     /locus_tag="AC16BC_06710"
     CDS             845..1519
                     /locus_tag="AC16BC_06710"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3241348.1"
                     /translation="MSDSLIRTENLRRSFVMGRDTIHVLRGVNLTIARGETVGLVGAS
                     GAGKSTLLHLLGGLDRPTDGAITVDGVRVDRLREAELEPFRNRTVGFIFQFHHLLPEF
                     TALENVMMPGLIRGLAQPEMRDRARHILDNVGLGARLAHRPGELSGGEQQRVAIARAL
                     VLEPPVVLADEPTGNLDTHTGEEVMAVLKRLRQARGTTFLIVTHNEKLALETDRVIRM
                     VDGVIE"
     gene            complement(1617..2687)
                     /locus_tag="AC16BC_06715"
     CDS             complement(1617..2687)
                     /locus_tag="AC16BC_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR3241349.1"
                     /translation="MITRRFGMAALAAVVAWVPVGADAFAAEYPVLFRGVRPLGMGGA
                     FLTLSNDENALFYNPAGLNDVKGFGGAGIVNPYAALSENSVQLYQDIQDLEGTDSAQV
                     ANLLNQHVGEHHHIQAAVFPHVYAHNFAVGVLGNGSMDLDIRNPAFPEVVTDVTIDAA
                     GLAGVAYGFWNQRLQVGVTGKYVRREGVVKTFQPADIVVDFDPLADRTMATDFAFDVG
                     TKVNLPVLLRPSLALVVENIGDLDFEGLGTIPQQINVGVGLHPDFWILSTTLAAEIHD
                     VTKQIEGDDDLYKRAHLGAELRFPRILAVQAGVNSGYYTAGVTLDFWILKLAAATYAE
                     ELGAYAGQRADRRNVVQLSLGF"
     gene            complement(2713..3483)
                     /locus_tag="AC16BC_06720"
     CDS             complement(2713..3483)
                     /locus_tag="AC16BC_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241350.1"
                     /translation="MTAMTHFTNRWITALVSGALMGVLSACGEQNLFDSLSDHDTAAA
                     KIEAAQIAVDNGDFTAAIATLETLCGTDSSAPTCDADTAALLGSAYAGRAGLNVFDLI
                     ATVTNTAPGTTSSFSSFSTLLPDPTVNNKNDLHDAVALLSSLANPTPNQSPELAVVAM
                     ADIVVTVGVDLTNGFDPTTGVPNTVPTLTDVQNAETASGTVTQVSNDIDLVVQGVTGS
                     GLANEDITNDINQVEDAIDTDQDGTVSAAELQAFLASL"
     gene            complement(3651..3998)
                     /locus_tag="AC16BC_06725"
     CDS             complement(3651..3998)
                     /locus_tag="AC16BC_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241351.1"
                     /translation="MVRLTRAVVVLLLVLGPLGCGGAGMSVMKKGTKASFSGEIDNIV
                     HTELPYDKEMLSVTAVGFASGQSVALIGLHPGLSKGKRIKVQAEFFENIRGTDVYRAI
                     QITTEPTAETRGQ"
     gene            complement(3998..4837)
                     /gene="trpA"
                     /locus_tag="AC16BC_06730"
     CDS             complement(3998..4837)
                     /gene="trpA"
                     /locus_tag="AC16BC_06730"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR3241352.1"
                     /translation="MRPSENRIDRTFARLRERGDKALIPYVMLGDPDVAATEQWAKAA
                     AAAGADLLEIGIPFSDPLADGPVLQRAAERALANKVGLGDALALVRRLRATVRIPLIF
                     MTYYNLVFQYGDERLAQDAAAAGVDGLIVPDLPPEEAGPLDAAARAVGLHLIYLIAPT
                     TPPARIALIAERARGFVYYVSLTGITGAKLPQLHEVEQGVARIKRATDVPVAVGFGVS
                     TPEEAGRIARVADGVIVASALIKRLHAQPDAARRLDELTAYVASLKAALSPRETFSTG
                     GSR"
     gene            complement(4834..>5643)
                     /gene="trpB"
                     /locus_tag="AC16BC_06735"
     CDS             complement(4834..>5643)
                     /gene="trpB"
                     /locus_tag="AC16BC_06735"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3241353.1"
                     /translation="LGLSCDVYMGTEDMARQSLNVFRMRLLGATVVPVEAGSRTLKDA
                     INEALRDWTTNVRTTHYILGSVLGPHPYPMMVRDFQSVIGREARRQILRLEGRLPNAL
                     VACVGGGSNAIGLFSGFLKDRGVRMIGVEAGGHGIATGQHAARFAAGRTGVFQGTKTV
                     VLQDPNGQIQITHSVSAGLDYSAVGPEHAWYRDLGRIEYTSVTDKEALEGFDLLSRTE
                     GIIPALESAHAVAHAAAVAPRMRTRDVMILNLSGRGDKDVQQVAKIRGIEL"
BASE COUNT          971 a         1929 c         1773 g          970 t
ORIGIN      
        1 gacggccgcc tcgcggacct gaccaccgcg tttcccccgc cggccgacaa gccggaactg
       61 tccccgcggt acggcatcat catcggcaag gaattggccc tgcgcctcgg cgtgttcacg
      121 ggcgacacgc tcaacgtggt gtcgccgcag ggcacgatgg gccccttcgg cgtggtgccc
      181 aaactgcgcc cctatcaagt ggtggggatc ttcgagtccg ggatgtacga ttacgattcc
      241 tcgttcgcgt acatctcgct cgcgcaggcc caggactttt tccaactggg cgacgccgtc
      301 accggcatcg agatcaaggt ggacgagatc ttccgcgccg gagacgtggc ctcggccgca
      361 gacgccgcgc tcggaggccg ctacttggtc cgcgactgga aagaaatgaa ccgcaacctg
      421 ttctccgcgc tcaagctcga aaagatcatc atgttcatca tcctggtgct gatcatcctg
      481 gtggccgcgt tcaacatcat cggcacgctc accatgatcg tgatcgagaa gtcgcgcgag
      541 atcgccattc tcaaggccat gggcgcgacc cggcgcgcgg tgatgggcat cttcatgctg
      601 gacgggctga tcatcggcgg cacgggcgtg gccatcggca tcccgctcgg ctacgcggtc
      661 tgctgggcga tcgaacattt ctacaccctc ccggccgacg tgtactacat cagccacatc
      721 ccggtgcgca tctacgcctt cgacgtggcg ctggtcgcgg gctccgcgct gatgatcacg
      781 ttcctggcca cgatgtaccc ctcgtggcag gccggccggc tcgcgcccgt ggaagccttg
      841 cggtatgagt gactcgctca tccgcaccga gaacctccgc cgatcctttg tcatggggcg
      901 cgacacgatc cacgtgttgc gcggcgtcaa tttgaccatc gcccgcggcg aaaccgtcgg
      961 gttggtgggc gcgtcgggcg cgggaaagag cacgctgctg cacctcttgg gcggcctgga
     1021 ccggccgacc gacggcgcga tcacggtgga tggcgtacgg gtcgatcgct tgcgcgaagc
     1081 ggagttggaa ccgtttcgca accgcaccgt ggggttcatc ttccaattcc accacctgct
     1141 gcccgaattc accgcgctgg aaaacgtgat gatgcccggc ttgatccgcg ggttggccca
     1201 accggagatg cgcgaccgcg cgcggcacat cctggacaac gtcggcttgg gcgcccgact
     1261 cgcgcatcga ccgggcgaac tctccggcgg cgaacagcag cgcgtggcca tcgcccgcgc
     1321 gctcgtcctc gaacccccgg tggtgctggc ggacgaaccc accggcaacc tcgacaccca
     1381 caccggggaa gaagtgatgg ccgtgctcaa acgtctgcgt caggcgcgcg gaacgacctt
     1441 cttgatcgtg acccacaacg agaaactggc gctggaaacc gaccgcgtga tccgaatggt
     1501 ggacggggtg atcgaataag accgaggggg agagtgggag aatgggtgag tgggcgataa
     1561 agatttagtg acttttcacc ccatcacccc gtctcccact ctccctgtgg ggcttttcag
     1621 aatccgagac taagttgcac cacattccgc cggtcggccc gctgcccggc gtaggccccg
     1681 agttcctcgg cgtacgtcgc ggccgcgagt tttaaaatcc agaaatccag ggtgactccc
     1741 gcggtgtagt acccgctgtt gaccccggcc tgcaccgcca ggatccgagg gaagcgcagt
     1801 tccgcgccga ggtgcgcgcg tttgtaaagg tcgtcgtccc cttcgatctg tttggtcacg
     1861 tcgtggattt ccgccgccag ggtcgtggaa agaatccaga aatccgggtg caggcccacc
     1921 ccgacgttga tctgctgcgg aatggtcccc aaaccctcga agtcgaggtc gccgatgttc
     1981 tccaccacca gggcgaggga tggacgcaag agcaccggca ggttcacctt ggtgccgacg
     2041 tcgaacgcaa agtcggtggc catggtccgg tcggccaacg gatcaaaatc caccacgatg
     2101 tccgcgggct ggaacgtctt gaccacgccc tcgcgtcgca cgtatttccc ggtcacgccg
     2161 acctgcagcc tctgattcca gaacccatac gccacccccg ccaggcccgc ggcgtcgatc
     2221 gtcacgtcgg tcacgacctc cgggaacgcg ggattcctga tgtccagatc catcgacccg
     2281 ttgcccagca cgccgaccgc gaagttatgg gcgtagacgt gcggaaacac cgcggcctgg
     2341 atgtggtggt gttcgccgac gtgctgattg agcaaattcg cgacctgcgc cgagtcggtt
     2401 ccttcaagat cctggatgtc ctgatacaac tgcacgctgt tctccgacag cgcggcatag
     2461 ggattgacga tcccggcgcc gccgaacccc ttcacgtcgt tgaggcccgc ggggttgtag
     2521 aacaaggcgt tttcgtcgtt ggagagggtc aggaacgcgc cgcccatccc caacggcctg
     2581 accccgcgaa acaacaccgg gtattccgcc gcgaacgcgt cggcgccaac cggcacccac
     2641 gccaccaccg cggccagcgc cgccatcccg aatcgacgcg tgatcatacg gctcgctccc
     2701 tccacgccct ggttacaggc tcgccagaaa cgcctgcagt tcggcggccg acacggtccc
     2761 gtcctggtcc gtgtcgatgg cgtcttccac ctgattgatg tcgttggtaa tgtcttcgtt
     2821 ggccaacccc gacccggtca cgccttgcac cacgaggtcg atgtcgttgc tgacctgtgt
     2881 gacggtcccg gaggcggttt cggcgttttg aacgtcggtc aacgtcggaa ccgtgttggg
     2941 aacgccggtg gtcggatcga acccgttcgt caaatccact ccgaccgtga ccacgatgtc
     3001 ggccatggcc acaacggcca gttccgggct ctgattgggc gtggggtttg ccagactcga
     3061 gagaagggcg accgcgtcgt gcaggtcgtt cttattgttc acggtcgggt ccggcaacaa
     3121 ggtggaaaaa ctcgagaaac tgctcgtggt cccgggcgcc gtattcgtca ccgtggcgat
     3181 caagtcgaag acattaaggc cggcgcgccc ggcatacgcc gaccccagca acgcggcggt
     3241 gtccgcgtcg caggtcggag ccgaggaatc ggtcccgcac aaggtctcca acgtggcaat
     3301 cgccgcggta aagtcgccgt tgtccaccgc gatctgagcc gcctcgattt tggcggcggc
     3361 ggtgtcgtga tcggagaggc tgtcgaacag gttctgctcg ccgcacgccg acaggactcc
     3421 catcagcgcg ccggagacca gagccgtgat ccagcggttc gtgaaatggg tcattgctgt
     3481 catggggcat tcctcacgcg ttcttcaaat gtcgccaccg gtgcccggat ccggctcact
     3541 gtaccggagc agaatggttt gtcaatcgaa gggaaacaga cgcgcgcgcc ggccgacgaa
     3601 ggcgcggaac gagtgaaacg gatcgggatc gggccctcat ccgggagggg ttactgcccg
     3661 cgagtctccg cggtcggttc ggtcgtgatc tgaatcgcgc ggtacacgtc ggtcccgcgt
     3721 atgttctcga agaactcggc ctgcaccttg atgcgtttgc ctttgctcag gccgggatgc
     3781 aggccgatga gcgccacgct ctggcccgag gcaaaaccca ccgcggtcac ggacaacatt
     3841 tccttgtcgt acggcaattc ggtgtgcacg atgttgtcga tctcgccgga aaacgacgcc
     3901 ttggtgcctt tcttcatgac gctcatcccg gcgccgccgc aacccagggg acccaggacc
     3961 agcaacaaca ccaccaccgc acgagtcaga cggaccatca tcgtgaacct cctgtcgaaa
     4021 acgtctcacg gggggaaagc gccgccttga gcgacgcgac gtacgcggtc aactcatcga
     4081 gacgcctggc cgcgtcgggc tgcgcgtgca gccgtttgat cagggcggac gcgacgatca
     4141 ccccgtccgc gacccgagcg atgcgaccgg cttcctcggg cgtggacacg ccgaacccca
     4201 cggccacggg cacgtccgtc gcgcgcttga tccgcgccac cccctgctcg acctcgtgga
     4261 gctgcggcaa cttggccccg gtgatgccgg tcaacgacac gtaatagacg aatccccgcg
     4321 cccgctcggc aatgagcgca atgcgcgcgg gcggcgtggt cggcgcgatc agatagatca
     4381 ggtgcagccc cacggcccgg gccgcggcat cgagcggccc ggcttcctcc ggcggcaggt
     4441 cgggcacgat caggccgtca accccggccg cggccgcgtc ctgcgccagc cgttcgtcgc
     4501 cgtactggaa caccaggttg tagtacgtca tgaagatcag cggaatccgc acggtcgcgc
     4561 ggagtcggcg gaccagggcg agcgcgtccc cgagtccgac cttgttggcg agcgcgcgtt
     4621 ccgcggcgcg ctgcaacacc gggccgtcgg ccagggggtc cgaaaacggg atgccgattt
     4681 ccagcaggtc ggcgcccgcc gcggccgcgg ccttggccca ctgctcggtc gcggccacgt
     4741 ccggatcgcc caacatcacg taggggatca acgccttgtc cccgcgctcg cgcaaccggg
     4801 cgaaggtccg atcgatgcga ttctcggacg gcctcacaac tcgatccccc tgatcttcgc
     4861 gacctgctgc acgtccttgt cgccgcggcc cgagagattc aggatcatca cgtcgcgcgt
     4921 tcgcatccgg ggcgccacgg ccgcggcgtg cgcgaccgcg tgcgcggact ccagggccgg
     4981 aatgatcccc tcggtgcggc tgagcagatc gaacccctcc aacgcctctt tgtccgtcac
     5041 cgacgtgtat tcgatccgtc ccaggtcgcg ataccacgcg tgctccggtc ccaccgcgga
     5101 ataatccagc ccggcggaca ccgagtgggt gatctggatc tggccgttcg ggtcctgcaa
     5161 caccacagtc ttggtcccct ggaacacccc ggtgcgcccg gcggcgaagc gcgccgcgtg
     5221 ttgcccggtg gcgatcccat gcccgcccgc ctccacgccg atcatccgca ccccgcggtc
     5281 cttcagaaac cccgagaaca ggccgatcgc gttgctccct ccgcccacgc aggccacgag
     5341 cgcgttgggc agccggccct ccagccgcag gatctggcgc cgcgcctcgc ggccgatcac
     5401 ggactgaaag tcccgcacca tcatcgggta cgggtggggg cccagcacgg aaccgaggat
     5461 gtaatgcgtg gtccgcacgt tggtggtcca gtcgcgcaag gcctcgttga tcgcgtcctt
     5521 gagcgtgcgg ctgcccgcct ccacgggcac caccgtggcc cccagcagcc gcatccgaaa
     5581 cacgttgagc gactggcgcg ccatgtcctc ggtgcccatg tacacgtcgc acgacagccc
     5641 gag
//
LOCUS       DBNGTT010000300         2612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2620860, whole genome shotgun sequence.
ACCESSION   DBNGTT010000300 DBNGTT010000000
VERSION     DBNGTT010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2620860"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="AC16BC_06740"
     CDS             <1..1171
                     /locus_tag="AC16BC_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR3241354.1"
                     /translation="LYGFAAAVLPVWLLLAPRDYLSTFMKIGTIVALAVAIVILNPEV
                     KMPALTQFIDGTGPIFGGNVFPFVFITIACGAISGFHSLVCSGTTPKMIEREGHALVG
                     YGAMLMESFVGVMALIAATILIPGDYFAINTTLPPDQLAALGFAPVEVGEFSRMVETN
                     VEGRPGGAVSLAVGMASIFAAVPGLRSLIAYWYQFALLFEALFILTTIDAGTRVARYL
                     IQEMGRAAWPRFADLNWTPGAVLSSGLVVAAWGYLIANGTIATIWPMFGAANQLLGTL
                     ALCMGTTLLIKMGQSRYVWITAAPMLFVGTVTLTASWQLFGVFSAKAAAATTAKAAFN
                     FRLDAALVAVVAILAVVILVDSARQWYGYLVQKRPYQSTEVASPEVYSLPTDPGC"
     gene            1180..2544
                     /gene="der"
                     /locus_tag="AC16BC_06745"
     CDS             1180..2544
                     /gene="der"
                     /locus_tag="AC16BC_06745"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3241355.1"
                     /translation="MARDLYPHPFVSFIGRPNVGKSTLFNRLIGHRVAIVQDEPGVTR
                     DRNYYPVEYCGSRFTLIDTGGITGEKSAAPGELGTARLLAEVQRQTDLAIEEADVIVY
                     LMDGREGLTPIDLDIAERLRKVRKPVWYAVNKIDGPKHDNKIIDFYRLGVAKLFPLSA
                     EHGYGVDDLLEAAIPKQERVVEAPEPETIPRVVVLGRPNVGKSTLINTLLGEERLVTS
                     DVPGTTRDTIDSLIERNGKIYRFIDTAGLRRRGKVNRGVEHFSVERTRDTLSRTDAAV
                     VLIDATEGVVEQETKIVGEALAAGRACVLVVNKWDLKPRGEDERTSFLTEVARRFTFL
                     EHAPVVFLSALKGTGVGQLFAAIDEVITSYTTRVTTGDLNRFFEAVVRDHPPPLSKGR
                     AVRLNYITQVGVKPPTFVVFASDAKAVSAHYRRYLENALRRRFGFRGTPIRIVVRSKR
                     GRSG"
BASE COUNT          424 a          830 c          861 g          497 t
ORIGIN      
        1 cctctacggc ttcgcagccg ccgttctacc ggtctggctg ctgctcgcgc cgcgcgatta
       61 cctgtcgacg ttcatgaaga tcggcacgat cgtcgcgctc gcggtcgcca tcgtgatctt
      121 gaaccccgag gtcaagatgc cggccttgac gcaattcatc gacggcacgg gcccgatttt
      181 cggcggcaac gtctttccgt tcgtgttcat caccatcgcg tgcggcgcga tctcggggtt
      241 tcattccttg gtctgttccg gcaccacgcc caagatgatc gaacgcgagg ggcacgccct
      301 ggtcgggtac ggtgccatgc tcatggagag tttcgtgggc gtgatggcgc tgatcgcggc
      361 cacgatcctc atccccggcg attactttgc gatcaacacc acgctgccgc ccgatcaatt
      421 ggccgcgttg ggttttgcgc cggttgaagt cggtgagttc tcgcgtatgg tcgagaccaa
      481 cgtggagggc cggcccggag gcgcggtgtc gctcgccgtg ggcatggcct cgattttcgc
      541 cgcggtgccg ggattgcgga gcttgatcgc gtattggtat cagttcgcct tgctcttcga
      601 ggcgctcttc atcctgacga cgatcgacgc gggcacgcgc gtggcgcggt atctgattca
      661 ggagatgggg cgcgccgcgt ggccgcgctt cgcggatctg aactggacgc ccggggccgt
      721 gctgtcgagc ggtctcgtgg tcgcggcgtg gggctatttg atcgccaacg gcacgatcgc
      781 cacgatctgg cccatgttcg gggccgccaa tcagctcttg ggcacgctcg cgttgtgcat
      841 gggcacgacc ttgctcatca agatgggcca atcgcggtat gtttggatta ccgcggcgcc
      901 gatgctcttt gtcgggacgg tcacgctcac cgcgtcgtgg caactgttcg gcgtgttctc
      961 ggccaaagcc gcggcagcga cgacggccaa agccgcgttc aacttccggc tggacgccgc
     1021 gctcgtagcc gtggtcgcca tcctcgcggt cgtgattctc gtggactcgg cgaggcagtg
     1081 gtacgggtat ttggtacaga agcggccgta ccagtccacg gaggttgcca gccccgaagt
     1141 ttattccttg ccgaccgacc ccggctgttg acccacgcca tggcgcgcga cctctaccct
     1201 cacccctttg tgtcgttcat cggccggccg aacgtgggca agtccacgtt gttcaaccgg
     1261 ttgatcgggc accgcgtggc cattgtgcag gacgaacccg gggtcacgcg cgaccgcaac
     1321 tactacccgg tggagtactg cgggagccgc ttcaccttga tcgacacggg agggatcacg
     1381 ggtgagaagt cggcggcccc cggcgaactc gggaccgcgc ggctcctggc cgaggtgcag
     1441 cgccagactg atctggcgat cgaggaggcc gacgtcatcg tgtatctgat ggacgggcgg
     1501 gaggggttga ccccgatcga cctggacatt gccgagcggc tgcgcaaagt ccggaagccc
     1561 gtgtggtacg cggtcaacaa gatcgacggt cccaaacacg acaacaagat tatcgacttc
     1621 taccggctcg gggtcgcgaa gctctttccg ctgtccgcgg agcacggcta cggggtggat
     1681 gatttgctgg aagccgcgat ccccaagcag gagcgcgtcg tcgaggcgcc cgaacccgag
     1741 accattccac gggtcgtggt gctggggcgc cccaacgtgg ggaagtccac attgatcaat
     1801 accttgctgg gcgaagaacg cttggtgacc agtgacgtgc ccggtaccac ccgcgatacc
     1861 atcgacagtc tgatcgagcg caacgggaaa atctatcgct tcatcgacac cgcggggctc
     1921 cggcgccgcg gcaaggtcaa ccgcggggtg gagcacttca gcgtggagcg cacgcgcgac
     1981 accttgtccc gaaccgacgc ggcggtggtg ctcatcgacg cgaccgaagg ggtggtggaa
     2041 caggaaacca agatcgtggg cgaagcgctg gccgcgggcc gcgcctgcgt gctcgtggtc
     2101 aacaaatggg atctcaagcc gcgcggcgag gacgaacgca cctcgtttct gaccgaagtc
     2161 gcgaggcggt ttacctttct cgaacacgcg cccgtggtgt ttctctccgc gctgaagggc
     2221 acgggcgtcg ggcaactctt tgccgcgatc gacgaggtca tcacgtccta caccacgcgt
     2281 gtgaccaccg gggacttgaa ccgctttttc gaggccgtgg tgcgcgacca ccccccgccc
     2341 ctgtccaaag gccgcgcggt acgcctgaac tacatcacgc aggtcggagt gaagcccccc
     2401 acgttcgtgg tgttcgccag cgacgccaag gccgtttccg cgcactaccg gcggtatctg
     2461 gagaacgccc tgcgccgccg gttcggcttt cgcggcaccc cgatccgcat cgtggtccgc
     2521 tccaaacgcg gccggagcgg ataggggttt cttctgaggt ttgtgtaagt cgccccccgc
     2581 aatcgggggc gagtccgttc agaagcgcgc ca
//
LOCUS       DBNGTT010000301         2841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1983542, whole genome shotgun sequence.
ACCESSION   DBNGTT010000301 DBNGTT010000000
VERSION     DBNGTT010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1983542"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1703
                     /locus_tag="AC16BC_06750"
     CDS             273..1703
                     /locus_tag="AC16BC_06750"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR3241356.1"
                     /translation="MVDSKTTDAATRTERDSMGEMRVPASALYGAQTQRAVENFPVSD
                     LRWPRSFIRALGLIKWAAAQVNRDLGLLPADKAEVVVRAAREVVDGQWDHQFVVDLFQ
                     TGSGTSTNMNANEVIANRATQLAGGAMGSKLIHPNDHVNLGQSSNDAIPTAIHIAALE
                     TIDRDLLPALTALHAALVQKSLEFARVVKIGRTHLQDATPVRLGQEFGGYARQIELGI
                     RRVENMRASLGELALGGTAVGTGINTHPEFAANVITLLARETDCQFFVEASDHFEAQG
                     AQDSLVEASGALKTLAVSLIKIANDIRWLGSGPRCGIGEITLPETQPGSSIMPGKVNP
                     VIAESLLQVCVQVIGNDAAITLGGQAGNFELNVMLPVMAYNLLQSIQLLAASARNFTT
                     KCVIGLTANEARCAAMIEQSLAMCTSLAPEIGYDHAAKIAKESYRTGKTVRQVALDQK
                     VLSPERLTVLLDPARMTERGFAGKGE"
     gene            1951..2427
                     /locus_tag="AC16BC_06755"
     CDS             1951..2427
                     /locus_tag="AC16BC_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241357.1"
                     /translation="MNRFLPAGTRNPMEDIMAFKARLDKDPKDVGALISLANANMVIS
                     RFDAAQDLYRRALDITPKNLDVRTNLAIAYKYGGKPAEAFADLQKNLAIDPQHGHTLY
                     NLGFLYLTDKQDRAKAVETWKTWLRLYPDDPGAAEISTRIAEFETQPKSGAARSGA"
BASE COUNT          500 a          914 c          960 g          467 t
ORIGIN      
        1 tcggcgcaag atggacgcgg cctcggccag ggcgtcggcg gtggtcacat aaatgaagtc
       61 cgtcgtgtcg gtcatcagcc gatatcgggg tctgtcaacg aatgggtctc cgggacatcg
      121 gaccgccgcg caccggcgcg gcggtcggca ggcccctgcg ttgcggaaat tataccggat
      181 atggggggcg gggtgcgagg cgagcgcccc ggggcttgtc acggcgcgta gtgtggtaca
      241 atccgcgcct gcggaaccca cggagacagg acatggttga ttccaagacc acggatgcgg
      301 cgactcggac cgaacgggat tcaatgggcg agatgcgcgt gccggcgtcg gcgctctacg
      361 gcgcccaaac ccagcgcgcg gtggagaact ttccggtcag cgatctgcgg tggccgcgca
      421 gcttcattcg ggcgttgggc ctgatcaaat gggccgcggc gcaggtcaac cgcgatctgg
      481 gtttgttgcc ggccgacaag gccgaggtgg tggtgcgggc ggcgcgcgag gtggtggacg
      541 ggcagtggga ccaccaattc gtggtggact tgtttcagac ggggtccggc acgtcgacca
      601 atatgaacgc caacgaggtc atcgccaacc gggcgaccca gttggcgggc ggggccatgg
      661 gctcgaagtt gatccacccc aacgatcacg tcaacctcgg gcaatccagc aacgacgcga
      721 tccccacggc cattcatatc gccgcgttgg aaacgatcga ccgcgacctg ctgccggcgc
      781 tcaccgcgct gcacgcggcg ttggtccaga aatcgctcga gttcgcgcgg gtcgtgaaga
      841 tcggccgcac ccacttgcag gacgccacgc ccgtgcgcct cgggcaggag ttcggcgggt
      901 acgcccggca gatcgaactg ggcattcggc gggtggagaa catgcgggcc tcgttgggcg
      961 agttggcgtt ggggggcacc gcggtgggga ccggcatcaa cacccatccc gagttcgccg
     1021 cgaacgtgat cacgctcttg gcgcgcgaaa ccgactgcca attcttcgtg gaagcgtcgg
     1081 atcatttcga agcgcagggc gcgcaggatt cgctggtcga ggccagcggg gcgctcaaga
     1141 ccctggccgt cagcctcatc aagatcgcga atgacatccg gtggctcggc tccgggccgc
     1201 gctgcgggat cggcgagatc accctgcccg agacgcagcc cgggtcgtcg atcatgccgg
     1261 gcaaggtgaa tcccgtgatc gcggaatccc tgctccaggt ctgcgtccag gtcatcggca
     1321 acgacgccgc gatcacgttg ggcggccagg ccggcaactt cgaattgaac gtgatgctgc
     1381 ccgtgatggc gtacaacctg ctgcagtcca ttcaactgct ggcggcgagc gccaggaatt
     1441 tcaccaccaa gtgcgtgatc gggttaaccg cgaacgaggc gcgctgcgcg gccatgatcg
     1501 agcaaagttt ggccatgtgc acgtcgctgg cgccggagat cgggtacgac catgcggcca
     1561 agatcgccaa ggaatcgtat cgtaccggca agaccgtgcg ccaagtggcc ctcgaccaaa
     1621 aggtcctctc tcccgaacgt ctgaccgtgc tgctggatcc cgcgcgaatg acggagcgag
     1681 ggttcgcggg caagggcgag taacgcagtt cgggaaacgt gacgcgacga atcgacaccg
     1741 cggcgcgcgg tcgctccgcc tcatcggcgc cgtcctgctc gccggactgg cgggtgtcca
     1801 gggttgccag aaaccacagg gcaacgggct gcccgagccg gtcggcgcgg gagcgccgac
     1861 cggctcggcg tctcttcgca aggcgcgctc ccgcccaatc atcccgccgt caccccgtct
     1921 ggcgcgcggt ctgatccctc cgctccggcg gtgaaccggt tcttgccggc cggaacccgt
     1981 aatcccatgg aagacatcat ggcgttcaag gcgcgcctgg acaaggatcc caaggacgtc
     2041 ggggccctga tttcgctggc caacgccaac atggtgatct cgcggttcga cgccgcgcag
     2101 gatctgtatc gtcgggcgct ggacatcacg ccgaagaacc tggacgtcag aaccaatctg
     2161 gcgattgcgt ataaatacgg cgggaagccc gccgaagcgt tcgcggactt gcagaagaac
     2221 cttgcgatcg atccccaaca cggccatacc ctctacaacc tcggcttcct gtatctgacc
     2281 gacaagcagg accgagcgaa ggccgttgaa acctggaaga catggctccg cttgtatccg
     2341 gacgacccgg gcgcggccga gatctcaacg cgaatcgctg aattcgagac ccaacccaag
     2401 tcgggcgcgg ctcgatccgg ggcgtagccc cgcctcctcg cgcctccgtg tgcgctcggc
     2461 gatcggccgg acgccggcgc tcccacccgt ccggggtatc cgccatgcag ccatgcgctg
     2521 aagcgggcca tccgggctag cgtccctggg aaagtttgtt attcttcctt aaatataatt
     2581 acgccggatt agggggcacc cgtccatcac atgctgatgt cgcacgccga gcgccacgca
     2641 cgcccaattg acttcgtccg tccggtgacg ggagcctgat gttgcccggc gttctgacgc
     2701 ggtcactcgc tccggccgtg gcgcgactcg ctggcgccgt catggtgatc ctcgcggcgt
     2761 gcggcggcgt gtcgtcggcg tgggccgaag acatcgcgat catcgggaac gcccgtggcc
     2821 cgttgaccac gctggggcgt g
//
LOCUS       DBNGTT010000302         2807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_321343, whole genome shotgun sequence.
ACCESSION   DBNGTT010000302 DBNGTT010000000
VERSION     DBNGTT010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_321343"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="AC16BC_06760"
     CDS             complement(<1..588)
                     /locus_tag="AC16BC_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3241358.1"
                     /translation="MSELERHITEKAQAAKRAAAALAVLSTEVKNRALRAMADALVAH
                     RAEILDANARDVETARRENRTNAFIDRLSLTPQRIDDMAKGLREVADLPDPVGAISSL
                     VVRPNGMQVGRMRVPLGVVGIIYESRPNVTADSAALCLKSGNAVLLRGGSESLQSNLA
                     IARLLTEAAAKAGVPEGAVSLIERTERDGVRVMATL"
     gene            complement(601..1752)
                     /gene="proB"
                     /locus_tag="AC16BC_06765"
     CDS             complement(601..1752)
                     /gene="proB"
                     /locus_tag="AC16BC_06765"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR3241359.1"
                     /translation="MSALDRNAVLRGARRVVVKVGSSVLASHARGVNTRRIGLLAREC
                     AALAREGYELALVSSGAIASGLAPLGLRERPRGLPLKQAAAAVGQSRLMWAYQRAFEA
                     QGRRVAQVLLTGDDLSRRARFLNARHTMMTLLELGVIPIINENDTVAVDEIKFGDNDT
                     LASLVVGLIDARLLVILSDVDGLYSGDPRRDPAAVRLVDVERITREVAAAAGGSSRSE
                     GTGGMVTKLSAAKRAASCGAGTLIIDGRRTGALSRALHGEPVGTMFFPTAEGLNSRQH
                     WIAHAVRVAGQLWLDAGAVRAVVETGKSLLPAGVTKVSGEFAAGDAVACLDSVGRVVA
                     RGLINYPASDMAKICGLKTSEIEKRLGYKYYDEVIHRDNLVVIDEASRT"
     gene            complement(1752..>2807)
                     /gene="obgE"
                     /locus_tag="AC16BC_06770"
     CDS             complement(1752..>2807)
                     /gene="obgE"
                     /locus_tag="AC16BC_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR3241360.1"
                     /translation="GMFVDYLSIQVKAGDGGNGCVSFRREAFVPRGGPNGGDGGHGGS
                     VIIRADPQLHTLLDLRYRGLYAAQRGEHGRGKNQSGKCGDDLVIRVPVGTLVRDAAQG
                     EVLADLTAADQEVVVARGGRGGRGNQHFATPTRQAPDFATPAGAGEERALTLELKLLA
                     DVGLVGLPNAGKSTFLSAVSAARPKIASYPFTTLEPALGVVTWAEGRSFVAADIPGLI
                     EGAHEGKGLGIQFLRHIERTAFLLHLVDVSDAAGGRPVEDFESVRRELALTSPELAAK
                     PFAVVATKLDAAGGDAVSVLRDYCGTHGWAFFEISAATGQGVQALLREVGSRVARADG
                     RTPDTDAPDAAVSSESC"
BASE COUNT          446 a         1038 c          889 g          434 t
ORIGIN      
        1 cagcgtggcc atcacccgca cgccgtcgcg ctcggtccgc tcgatcagcg acaccgcgcc
       61 ctcgggaacg cccgccttgg cggccgcctc ggtcaggagt cgcgcgatcg ccaggttgga
      121 ttgcagcgat tcggaccctc cgcgcaacag cacggcgttt ccggatttca ggcacaacgc
      181 cgcggaatcc gcggtcacgt tgggacgcga ctcgtagatg atcccgacga cgcccagcgg
      241 cacccgcatc cggcccacct gcatcccgtt gggccgcacc accagcgacg agatcgcgcc
      301 caccgggtcg ggcaagtccg cgacttcgcg cagtcccttg gccatgtcgt cgatgcgttg
      361 gggcgtgagg ctgaggcggt cgatgaacgc gttggttcga ttctcgcgcc gggccgtctc
      421 cacgtcgcgg gcgttcgcgt cgagaatctc cgcgcggtgc gcgacgagcg cgtccgccat
      481 ggcccgcaac gcccggttct tgacctcggt ggacaacacc gccagcgccg cggccgctcg
      541 tttggcggct tgggccttct ccgtgatgtg gcgctccaac tcgctcatgt tcgcgtcccg
      601 ctaggtccga ctggcttcgt cgatgacgac aagattgtcg cggtgaatga cctcgtcata
      661 atatttgtat cccagccgtt tttcaatctc ggaggtcttg aggccgcaaa tcttggccat
      721 gtccgacgcc gggtaattga tcaatcctcg ggccacgact cgccccacgg agtcgagaca
      781 cgccaccgcg tcgcccgcgg cgaactcgcc ggacaccttg gtcacgccgg ccggcaacaa
      841 actcttgccg gtctccacga ccgctcgaac cgcgcccgcg tcgagccaca gctgcccggc
      901 gacgcgtacc gcgtgggcga tccagtgctg ccggctgttc aaaccctcgg cggtggggaa
      961 gaacatcgtg cccacgggct ccccgtgaag ggcgcggctc aacgccccgg tccggcgccc
     1021 gtcgataatc agggtgccgg caccgcacga cgccgcgcgt tttgccgcgc ttagtttggt
     1081 caccatcccc cctgtgccct cgcttcggct cgacccgccc gcggccgccg ccacctcgcg
     1141 tgtaatccgc tccacatcga ccaagcgcac ggcggcggga tcacgtcgag ggtcgccgct
     1201 gtataacccg tccacgtcgg acaggatcac cagcaggcgc gcatcgatga ggccgacgac
     1261 caggctcgcc agcgtatcgt tgtcgccgaa cttgatctcg tccaccgcga ccgtgtcatt
     1321 ctcgttgatg atcgggatca cgccgagttc cagcagcgtc atcatcgtat ggcgggcgtt
     1381 gagaaagcga gcgcgccgcg acaggtcgtc accggtcaac agcacctgcg ccacgcggcg
     1441 tccctgcgcc tcgaacgccc gctgataggc ccacatcaga cggctttgcc cgaccgcggc
     1501 cgcggcctgc ttgaggggca gaccgcgggg gcgttcgcgc agacccagag gcgccagacc
     1561 ggacgcgatc gcgccggagg acaccagcgc gagctcgtat ccctcgcgcg ccaaggccgc
     1621 gcattcccgc gcgagcagcc cgatgcggcg ggtgttcacg ccgcgcgcgt gcgaggccag
     1681 cacgctgctg ccgaccttta ccaccacgcg tcgcgccccg cggagcacgg cattccggtc
     1741 caaagcggac atcagcagga ctccgaggac acggctgcgt cgggcgcatc cgtgtccggc
     1801 gtccggccgt ccgcgcgggc cacccggctg cccacctccc gcagaagcgc ctgaacccct
     1861 tggcccgtcg ccgcggagat ctcaaaaaag gcccacccgt gggtgccgca gtaatcccgg
     1921 aggacgctca ccgcgtcgcc gccggccgcg tcaagtttgg tcgccaccac cgcgaacggc
     1981 ttggccgcga gttcaggact ggtcagcgcc aactcccgtc gcacggactc aaagtcctcg
     2041 accggcctac cgcccgccgc gtccgacacg tccaccaaat gcagcagaaa ggcggtgcgc
     2101 tcgatatgac ggagaaattg aatgcccagc cccttgcctt cgtgcgctcc ctcgatcaaa
     2161 cccgggatgt ccgccgccac aaagctccgg ccctcggccc acgtcaccac gccgagcgcg
     2221 ggctccaacg tcgtgaatgg gtaggacgcg atcttgggcc gcgcggccga gaccgcggac
     2281 aggaaggtgg atttcccggc gttcggcaaa ccgaccagac cgacgtcggc gaggagcttc
     2341 agttccaacg tcaacgcgcg ctcctccccg gctccggcgg gcgtggcgaa gtccggcgcc
     2401 tgccgcgtgg gggtggcgaa gtgctgattg ccgcgcccgc cgcgcccgcc gcgggcgacc
     2461 acgacctcct gatcggccgc ggtcagatcg gccaacacct cgccctgcgc cgcgtcacgc
     2521 acgagcgtgc cgaccggcac gcggatcacc agatcgtcgc cgcatttgcc gctctgattc
     2581 ttgccgcgcc cgtgttcgcc gcgttgagcc gcgtacaacc cgcggtagcg cagatcgagc
     2641 aaggtgtgga gttggggatc ggctcggata atgacgctgc caccgtggcc gccgtcgccg
     2701 ccgttggggc cgccgcgcgg aacaaaggct tcacggcgga aactgacgca tccgtttccg
     2761 ccgtcgccgg ccttgacttg aatggagaga tagtcgacga acatacc
//
LOCUS       DBNGTT010000303         3588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2876815, whole genome shotgun sequence.
ACCESSION   DBNGTT010000303 DBNGTT010000000
VERSION     DBNGTT010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2876815"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..730)
                     /locus_tag="AC16BC_06775"
     CDS             complement(65..730)
                     /locus_tag="AC16BC_06775"
                     /inference="COORDINATES: protein motif:HMM:NF015510.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR3241361.1"
                     /translation="MNPGRPLLALACLAVWTLGASAPTSPADVTSGVRDTYQSLTDLS
                     ADFTQATTVPGFATTLQSKGKVFLTKGKMRWDYTEPSDQRIYVNEGRIQYYIPAHKQV
                     IISALTPENDDQVPLHLLMTLERLDQDFNVSWENPASPRRDAEYRLRLDPKRPMADLE
                     YVTVGVDAQRRVVTSIALHPKNGNVSSFVFSQLKVNPRLDAALFSFSVPEGIEVVETP
                     TLR"
     gene            complement(727..2925)
                     /locus_tag="AC16BC_06780"
     CDS             complement(727..2925)
                     /locus_tag="AC16BC_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR3241362.1"
                     /translation="MPVAGMSRERVQEILGVTAATASVFLAVSLLSHTSADPSLISTS
                     HAPVQNLAGRVGANLSEALFQFAGASAYLLPVFTVLAAWRGLVGKRALWTWRGAGLAA
                     LLGLFLSTVTELYLPEGDFYGGGVLGAAMSGLLLGAFAWWGTLIVLTAASLVAVLALF
                     PWSLREGAGRTGSLLSSLVNRVRGMFVIRRGRTRRKQAAPTVRQADAPPQPPPRIAEA
                     PEPTPAPQRQESLAFMRGHANYQLPPLSLLADAPSVSRKAGKDDLVMSSRLLEKKLQD
                     FGVQGAVTEVHPGPVITMYEVELEPGVKVSKVLNLADDLALAMKAPSVRIVTPLPGKS
                     AIGIEIPNQVRETVALKEIVSATEFLDTPGRLPLALGKDTVGQPVVADLAAMPHLLVA
                     GATGSGKSVALNTMILSLLSAGGPRDIRMLMIDPKMLELSAYDAIPHLLAPVITQPKD
                     ASAALKRVVAEMQRRYRLLAEAGVRNIDGYNKQVESTPIPPASEVASSKSGVEVSVTA
                     APPERLPYIVVIIDELADLMLVAAREVEDSIMRLAQMARAAGIHLILATQRPSVDVLT
                     GVIKANFPARISFQVSSKTDSRTILDANGAEQLLGRGDMLYLMPGTGRIARIHGAFVS
                     DVEIASTVEFVKAQGPPGYEWSLEEADASGFRGGEDPERDDLYEKAIDLVATTGQASA
                     SFIQRRMRVGYPRAARMIEMMEEDQIIGPASGGKPREVLVRRAVPGEAEA"
     gene            3300..>3588
                     /locus_tag="AC16BC_06785"
     CDS             3300..>3588
                     /locus_tag="AC16BC_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241363.1"
                     /translation="MAIAAMPWAVTAFGNSIKGSRHDLSGLNMRGYGVETGPMTGATF
                     NDYKEVCVYCHTPHNADGAAPLWNRDLPAQSGYLMYSSPNFDSKAPAPDGIS"
BASE COUNT          610 a         1245 c         1150 g          583 t
ORIGIN      
        1 aggtacgtga ggagcggatg cgcagcggca acgcagcaga tgaccggtca tggacgggct
       61 tcgcctaccg gagggtcgga gtttcgacca cttcaattcc ctccggcacg ctgaacgaga
      121 acaacgccgc gtccagccgc ggattcacct tgagctggga aaacacgaac gacgagacgt
      181 tgccgttttt cgggtgcaag gcgatcgagg tcaccacccg gcgctgcgcg tccacgccca
      241 ccgtgacgta ctccaaatcc gccatcggac gcttgggatc gagccggagc cggtactccg
      301 cgtcccggcg cggcgacgcc ggattctccc acgacacgtt gaagtcctga tccaaccgct
      361 ccaacgtcat gagcaggtgc aggggaacct ggtcgtcgtt ctcgggcgtc agcgcgctga
      421 tgatcacttg cttgtgcgcg gggatatagt actgaatcct cccctcgttc acgtagatgc
      481 gctgatcgct gggttcggtg tagtcccacc gcatcttgcc cttggtcagg aaaaccttgc
      541 ctttggattg cagggtggtg gcgaatcccg ggaccgtggt ggcttgcgta aagtccgcgg
      601 acaaatcggt caacgactga tacgtatcgc gcacgccgga cgtgacgtcc gcgggcgagg
      661 tcggcgccga cgcgccgaga gtccagacgg ccaggcacgc gagcgccagg agcggccggc
      721 ccgggttcac gcctccgcct cgcccggcac cgcgcgccgc accagcactt cccgcggctt
      781 gcccccgctg gcggggccga tgatctggtc ctcttccatc atctcgatca tgcgggccgc
      841 ccgcgggtaa cccacgcgca tgcggcgctg gataaacgac gcggacgcct ggccggtggt
      901 ggccaccagg tcgatcgcct tttcgtacaa atcgtcgcgt tccggatctt ccccgccccg
      961 gaacccggac gcgtcggcct cttccagtga ccactcgtat ccgggcggtc cctgcgcctt
     1021 gacgaactcc acggtcgacg cgatttcgac gtcggacacg aacgcgccgt ggatgcgcgc
     1081 gatccgcccg gtccccggca tcagatacaa catgtcgccg cggcccaaca gttgctcggc
     1141 cccgttcgcg tcgaggatcg tgcgcgagtc ggtcttggac gagacttgaa acgagatgcg
     1201 cgccgggaaa ttggccttga tcacgccggt gagcacgtcc accgacggcc gctgcgtggc
     1261 caggatcagg tgaatgccgg ccgcgcgcgc catttgcgcc agccgcatga tggaatcctc
     1321 cacctcgcgc gcggccacca gcatcagatc cgcgagttcg tcgatgatca ccacgatgta
     1381 cgggagccgt tcggggggcg cggccgttac cgacacctcg acgcccgatt tcgacgaggc
     1441 gacctccgac gccggcggga tcggcgtcga ctccacctgc ttgttgtagc cgtcgatatt
     1501 gcgcaccccg gcctcggcca gcaatcgata ccgccgctgc atttcggcca ccacccgctt
     1561 gagcgcggcg gacgcgtcct tgggctgcgt gatgaccggc gcgagcaggt gcgggatcgc
     1621 gtcgtacgcg gacaactcca gcatcttcgg atcgatcatc aacatgcgga tgtcgcgcgg
     1681 ccctccggcc gacaacaggc tgaggatcat ggtgttgagc gccacgctct tgcccgatcc
     1741 cgtggccccg gccacgagca ggtgcggcat ggccgcgagg tccgcgacca cgggctggcc
     1801 cacggtgtcc ttgcccaacg ccagcggcaa ccgccccggc gtgtcgagga attcggtcgc
     1861 ggacacgatt tccttgagcg ccacggtttc gcgcacctgg ttggggatct cgatcccgat
     1921 cgcggacttg cccggcagcg gggtgacgat ccgtacgctc ggcgctttca tcgccagcgc
     1981 cagatcatcg gccagattga gcaccttgct gaccttcacg cccggctcca actccacttc
     2041 gtacatggtg atcacgggcc cggggtgcac ctcggtcacc gcgccctgca cgccgaagtc
     2101 ctggagtttc ttctccagga gccgggaact catcaccagg tcgtcttttc cggccttgcg
     2161 cgagacgctc ggcgcgtcgg ccagcaacga caagggcggc aattggtagt tggcgtgacc
     2221 ccgcatgaac gccagacttt cctggcgctg cggggccggc gtgggttccg gggcctcggc
     2281 gatgcgcggc gggggctgcg gaggcgcgtc ggcctggcgc acggtcggcg cggcctgttt
     2341 gcggcgcgtg cgcccgcggc ggatgacgaa catgcccctg acccggttca cgagcgacga
     2401 caacaggctt ccggtgcggc ccgcgccctc ccggagcgac cacgggaaca atgccagcac
     2461 cgcgacgagg ctcgccgcag tgagcacgat cagggtcccc caccacgcga acgcccccaa
     2521 caacaggccg gacatcgccg ctcccaacac cccgccgccg tagaagtcgc cctcgggaag
     2581 gtacaactcg gtgacggtgg agagaaagag cccgagcaac gcggccaggc ccgcgccgcg
     2641 ccacgtccag agcgcgcgct tgcccaccag gccgcgccag gcggccaaca ccgtgaatac
     2701 cgggaggagg tacgcgctcg cgcccgcgaa ctgaaagagg gcttcggata aattggcccc
     2761 gacgcgaccg gcgagattct gcaccggggc gtgagacgtg gaaatgagcg acgggtccgc
     2821 cgaggtgtgc gacaggaggc tgacggccag gaacaccgac gccgtagctg cggtcacacc
     2881 caggatttcc tgcacccgct cgcgagacat cccggcgaca ggcatggaga cgcagtatag
     2941 caaaggccgc gcgtcggttt caacaaaccg gcggaggccg cgcccacggc cgccgccgga
     3001 gcgcttccag accccgcccc tcgagcgtgg cgcgacaaca tgccgccccg cggcccgcgg
     3061 gcaggatttt cgcttgactt ccccttggtc ccttccctat attgaggctt ctttttgctc
     3121 tggaatatca agggagtggt gcgcacctgg ggaatttttg ccactccctg cggtcttagc
     3181 agtacgtcat tgaggcgtcc tgtcctcgcg ccggtgagtg gtgctgcgcc cggagcccgg
     3241 ccgccaggca attgctacgg aatatgattc gcccgcgtcg aatcttcgca atcacggccg
     3301 tggcgatcgc cgcgatgcca tgggcggtca ccgcgttcgg aaattccatc aaaggctccc
     3361 gccacgacct gtcgggcctg aatatgcgcg gctacggggt ggaaaccgga ccgatgacgg
     3421 gcgcaacctt caacgactac aaagaagtct gcgtctactg tcacacgccc cacaacgccg
     3481 acggggccgc cccgctctgg aaccgcgacc tgccggctca aagcggctac ctgatgtact
     3541 cgtcgccgaa cttcgattcc aaggccccgg cgcccgacgg catttccc
//
LOCUS       DBNGTT010000304         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_578533, whole genome shotgun sequence.
ACCESSION   DBNGTT010000304 DBNGTT010000000
VERSION     DBNGTT010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_578533"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /gene="galT"
                     /locus_tag="AC16BC_06790"
     CDS             <1..1006
                     /gene="galT"
                     /locus_tag="AC16BC_06790"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR3241364.1"
                     /translation="VMPELRKDPIVERWVIISTERWKRPSDFKVSREIPGRGSLCPFC
                     PGNEQLTPSEVLAYRPDAGRSNAAGWTLRVVPNKFPALQIEGKLNREGVGMFDKMNGI
                     GAHEVIIETPDHKATFSTMPDKALEDLFWAYRDRIMDLRKDSRFRYILIFKNQGAEAG
                     ASLEHPHSQLIALPIIPSHVLEELHGAKEHFAEKERCIYCDIVKQEMTDGSRVVCENG
                     DFVCLAPFAPRFPFEVWILPKTHASNFEEAQKSHIESLARIFSEVLRRLDRALDTPPY
                     NFILHTSPLRDKTDDYHHWHFEIMPKLTELAGFERGSGFYINPTPPEEAAKFLREVEF
                     "
     gene            complement(1025..1441)
                     /locus_tag="AC16BC_06795"
     CDS             complement(1025..1441)
                     /locus_tag="AC16BC_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241365.1"
                     /translation="MFKWLRDWIAAFQNEPGETPASTAFGATRRSLKEPAKKLVKSDR
                     TPEQAQQHKAEALTKLDEAKALGLLDSWTIEHDVPMLYVGPAWNNADPKAKKAFLQHA
                     NNYFLDGQEGFIAFDIFDAKTRKKIARWGNFEPYVD"
     gene            complement(1465..>2664)
                     /gene="serS"
                     /locus_tag="AC16BC_06800"
     CDS             complement(1465..>2664)
                     /gene="serS"
                     /locus_tag="AC16BC_06800"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3241366.1"
                     /translation="LRQRDEQRRRLQQEIDGLKQRRNDVSDRIARLKRDKAPASDVDA
                     LREGLQQEGDALKRLESEWQAVNGEVETALLTLPNLPHESVPEGRDGSANVEIRRIGV
                     PRDFPFTPQAHWELGERLGILDFERGAQITGSRFTVYRGAGAHLERALINFMLDRHTR
                     EHGYTEILPPVIVNRASMTGTGQLPKFEDDLFRLAAEDYFLIPTAEVPLTNLHRDEIL
                     GEPELPIAYTAYTPCFRREAGSYGKDVRGLIRQHQFNKVELVMLTTPERSYDALERLT
                     RHAEAILSALELPYRVMMLCGGDLGFGAAKTYDLEVWIPSERTYREISSCSNFEAFQA
                     RRIGIRYRPAAGKKTEFVHTLNGSGLAVGRTVVAILENYQRADGTVEIPKALRPYMDG
                     VDAIRAS"
BASE COUNT          462 a          826 c          852 g          524 t
ORIGIN      
        1 ggtaatgcct gaattgcgca aggatccgat cgtcgaacga tgggtgatca tttccaccga
       61 gcggtggaag cggccctcgg acttcaaggt cagccgggag attcccgggc ggggatcgct
      121 ctgcccgttc tgccccggca acgagcaact cacgccgtcc gaggtgctgg cctaccgtcc
      181 ggacgccgga cgttccaacg ccgcggggtg gacgctgcgg gtcgtgccca acaagtttcc
      241 ggcgctgcag atcgagggca aactcaatcg tgaaggcgtg gggatgttcg acaagatgaa
      301 cggtatcggc gcgcacgaag tcatcatcga gacccccgat cacaaagcca cgttttccac
      361 gatgccggac aaagcgctcg aggacttgtt ttgggcgtac cgcgaccgca tcatggacct
      421 gcgcaaggat tcgcgctttc ggtacatcct cattttcaag aatcagggcg ccgaagccgg
      481 cgcgtcgctc gaacaccccc attcccaact gatcgccctg ccgatcatcc cgagccacgt
      541 cttggaagaa ttgcacgggg ccaaggagca ctttgcggaa aaagagcggt gcatctactg
      601 cgacatcgtc aaacaggaga tgaccgacgg cagccgggtc gtgtgcgaga acggcgactt
      661 tgtctgcctg gcgccgtttg cgccccgttt cccgttcgag gtgtggattc ttcccaagac
      721 acacgcgtcc aacttcgagg aggcgcagaa atcccacatc gagtcgctcg cccggatctt
      781 ctccgaggtt ttgcggcggc tggaccgcgc gctcgacacg cccccgtaca actttatcct
      841 ccacacctcg ccgttacgcg acaagacgga cgactaccac cactggcact tcgagatcat
      901 gccaaagttg accgagctgg cgggattcga gcgcggatca ggcttctaca tcaacccgac
      961 cccgcccgag gaagcggcga agttcctgcg ggaagtggag ttctagcctc gccgggcgat
     1021 caggtcagtc gacgtacggc tcgaagttgc cccagcgcgc gatttttttc ctggtcttgg
     1081 cgtcgaagat gtcgaaggcg atgaagcctt cttggccgtc caggaagtaa ttgttggcgt
     1141 gttggagaaa ggctttcttg gccttggggt cggcgttgtt ccaggccggg cccacgtaga
     1201 gcatcggcac gtcgtgttcg atggtccagg agtccagtaa gcctagggcc ttggcttcat
     1261 cgagcttggt cagggcttcg gccttgtgtt gctgggcctg ctcgggcgtg cggtcggatt
     1321 tgaccagctt cttggcgggc tccttgagcg agcgtcgggt ggcgccgaag gcggttgacg
     1381 ccggggtttc acccggctcg ttttgaaagg ccgcgatcca gtccctgagc catttgaaca
     1441 cgctggtcct ccttggtggt tcggttacga cgcgcgaatc gcgtccacgc cgtccatgta
     1501 gggacggagc gctttgggga tctccacggt gccgtcggcg cgctggtaat tttccaaaat
     1561 cgcgaccacg gtgcggccga ccgccaggcc cgatccgttg agcgtgtgga cgaactcggt
     1621 cttcttgccg gccgccggcc ggtagcgaat cccgatccgc cgcgcctgga aggcctcgaa
     1681 gttgctgcac gaagagatct cccggtacgt ccgttcgctg gggatccaga cttcgaggtc
     1741 gtacgttttc gcggcgccga agccgagatc gcccccgcag agcatcatca cgcgatacgg
     1801 cagttccagc gcagagagaa tcgcttcggc gtgccgggtg aggcgctcca acgcgtcgta
     1861 cgaacgctcg ggcgtggtca gcatcacgag ctcgacctta ttgaattggt gttggcgaat
     1921 caatccccgc acgtcttttc cgtacgagcc ggcctcgcgg cgaaagcacg gcgtgtaggc
     1981 cgtgtacgcg atgggcagct cgggttcccc cagaatctcg tcacggtgca gattggtgag
     2041 cggcacctcg gcggtcggga tcaagaaata gtcctccgcg gccaagcgga acagatcgtc
     2101 ctcgaatttc gggagctgcc cggttccggt catgctcgcc cggttcacaa tgaccggcgg
     2161 gaggatttcg gtgtacccgt gctcccgggt atggcgatcg agcatgaaat tgatcagcgc
     2221 gcgttccagg tgcgcgcccg ccccgcggta caccgtaaac cgcgacccgg tgatttgcgc
     2281 cccgcgttca aagtccagaa tgccgaggcg ctcgccgagc tcccagtgcg cttggggcgt
     2341 gaacgggaag tcccggggca cgccgatacg acggatttcc acgttggccg atccatcccg
     2401 gccttcggga accgactcgt gcggaagatt gggaagggtc agcagcgcgg tctccacctc
     2461 gccgttgacc gcctgccact cggattccag gcgtttgagc gcgtcgcctt cttgctggag
     2521 gccctcgcga agcgcatcga cgtcgctcgc cggggccttg tcgcgcttta agcgcgcgat
     2581 gcgatcggag acgtcgttgc gccgctgctt gaggccatcg atctcctgct ggagccgccg
     2641 gcgttgctcg tcgcgttgcc gcag
//
LOCUS       DBNGTT010000305         5957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2750823, whole genome shotgun sequence.
ACCESSION   DBNGTT010000305 DBNGTT010000000
VERSION     DBNGTT010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2750823"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..2834
                     /locus_tag="AC16BC_06805"
     CDS             231..2834
                     /locus_tag="AC16BC_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3241367.1"
                     /translation="MVHYYPLLISLAPLTAALLTALPGSGIGPKVFRVGVWAHAVAFG
                     IALLVLREAAGPGYEPIRLPLVSSAWNGLLQFDLYIDRLAAVMMTLVTGVTLIIYLFA
                     VRYMQPEHGRARFHTLLSLTTVVLLGMVTSANLLMLVLFWQLLSWLVSFLAFNYAHPP
                     TAQGAFKTFTMLRVGDIAFLAGIVLAYHLYGTLDLPQLFARAAEAGAMVSLWPGGGLE
                     IRATTAITLLIFVGAMSKSAQFPLHGWLPDSLYAPTPVHALLHAGIINAGGFLLNRLA
                     PLYGLSPGTLHVVFAVGLLTALLGAGMMLTQNDIKKTLGYSTIGQMGYMIMECGLGAF
                     ALAVFHLIAHGLFKGTIFLNCGNVIHAARQEPRLPHQRATDEQTDFSLLTWVTGFAAT
                     LILPLIILLAAHGVLDLTFRDAQGMVIFLFFAWVTSSQAILTLYRLRAVNSPKVAALM
                     LVTLLLVVVTYVLAGERFTYFLYPNAGEVAGYFHSAALPGWLFDLIVAVTALFVIMGW
                     TLMYARSHGRVIRIPDWIDTVRVRVYLLLINRLYVDALSLRVGRRVRSIAHRVDRNAL
                     TPVVVGVLALGASAPALAMAADLPVGTVVGLVLAAILLPLFPLHGAYVAALTRVPGPI
                     AVGLAFVLPIAGLALVMNLLPAIPAELLGGVGVLAVVGALYGSFKALVQARVMHLLAY
                     AGLALLSIAWWHLAAVGRPTNRAVAFVCAVGLVIGGMCFAWQRVRARYGELDPDRISG
                     LAQPMPRFAVLLALLVMAGVGLPPFGLFAGFFAMVVDPSVAGTWGLGAMLATWLAASW
                     YLFKLIQRLLFGPHRADILYEDFRGAEVASLALIVLVLAALGVAPYAFLDADMLAGLA
                     FSALDLSTWNP"
     gene            2822..5950
                     /locus_tag="AC16BC_06810"
     CDS             2822..5950
                     /locus_tag="AC16BC_06810"
                     /inference="COORDINATES: protein motif:HMM:NF021578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HIR3241368.1"
                     /translation="MEPVTAAYSDTQRMQLRALILLASEIVASYWPMRIFVHHNPLHG
                     LEDLPFDEAVRRAHRFLGGKGYLSNARFRDYAGSGRIRSRHIDAALKPLARDKRVTIG
                     GREVTHLDALRAHLLHGIAEPDRDTVEPLIDRRTDRAMIAALAARLGPVLHASRVADR
                     QESEAAQAGAALVRRSTLAAWCDRVLRTQLTERINREMIKWCEAFLDEDHAAWAMPGR
                     EQSFYGAWRVLAQQEWSPCGIADSRRKIARLPPAPEDALLQHLAALGIPSQGRQEYLT
                     LHLTALPGWAGFIKWRADQSESAWQQTFPADLVQYLAVRLWYERELVQEACREGLGID
                     GTVSAIAAAVSTRDDAQTPGLDQDAAMRGAAALRLLALAAPLAIDPATMIAAPPEDLK
                     ALLDWIDGFPVSEHGPVWLNAFEAGYRDQLIETLRRGRSNSEAGSDAEQPAVRPQAQA
                     VFCIDVRSESFRRHLEAVGDFGTYGFAGFFSVFIRYRALGAHHDTDQFPVIMKAKNTV
                     REVPRTYQGALLSRRQAGARMLDAGHSLLHDLKENVVTPYVMVESLGWFYSLPMIGKT
                     VFTSWYRTWTAWLRRKVVPVISTAMTVDKLSREEVDEMLAAEQRSVIRRALQERFGDR
                     DLNVSLERLEGLRRRALDEQAVPATSPEARRGYALSPEEETWFVDVLRRDYRINQGGA
                     FARMERITQIGFTLAEQVFTVETALRVMGLTKNFARLVLCCGHGSTSDNNPFEAALDC
                     GACGGNPGEPNARVLAAMANKAQVRDALAKAGIVIPQDTYFIAGRHDTTTDEVRLFDL
                     EDLPHTHRNDMLRLEAALFEAGLRNNRERCARFPEMPEPLTTKRAAKEVRRRGSDWSQ
                     VRPEWGLSGNAAFIIGRRDLTRGLPLDARVFLHSYDYQGDPTGRLLEIVMTGPQVVGQ
                     WINMEHYFSTVDPEVYGSGSKIYHNVVGRFGVMSGPQSDLRTGLAAQTVMDGPRPYHE
                     PMRLLTLIEAPTDRITQVIRRHRVLQRLYDNEWVRLAALDPDTRIFYYYVPRQGWTVM
                     NDGGPSPHTHAS"
BASE COUNT          874 a         1930 c         2017 g         1136 t
ORIGIN      
        1 aactttacgg aagatagccc aaaggattcc aacggaatca gttgcgctat aggccgactc
       61 cctcacaata ggcgagtcgg ccttttattg acttggtaga ccggctgcag aacaacccta
      121 ggcgtgattc ggggtggttc agcggagggt aggccagcac gagtcccttc acgggatcga
      181 cgctggcctt tttattttgt ggtgcgatcc ggggatccat catcacgatg gtggttcact
      241 actatccgtt gctgatttcg ctggcgccgc tcaccgcggc cctcctgacc gccctcccgg
      301 gatcgggcat cgggcccaag gtgtttcggg tcggcgtgtg ggcgcacgcc gtggccttcg
      361 ggattgcctt gctcgtgttg cgcgaggccg cggggcctgg gtacgagccg atccgcttac
      421 ccctggtgtc ctcggcgtgg aacggcttgc tgcagttcga cctgtacatc gaccggttgg
      481 ctgccgtgat gatgacgctt gtcaccgggg tcaccctcat catctacctg tttgccgtgc
      541 gctacatgca gccggaacac gggcgcgccc gctttcacac cctgctctcc ctcacgaccg
      601 tggtgctgct cggcatggtg accagcgcca atctgttgat gctggtgctg ttctggcaac
      661 ttctgagttg gctggtgagc ttcctggcat tcaattacgc gcatcccccc acggcccaag
      721 gcgcgttcaa gacctttacc atgctgcgcg tgggcgacat cgcgttcctc gccggcatcg
      781 tcctcgcgta tcacctgtat gggacgctgg atctgccgca gctcttcgcg cgcgccgcgg
      841 aagccggggc catggtgtcg ctctggcccg gcgggggatt ggagatccgc gccacgaccg
      901 cgatcacact gttgatcttc gtcggcgcca tgagcaagtc ggcgcagttc ccattacacg
      961 gctggctccc ggattccctc tacgcgccga ccccggttca cgcgctcctg cacgcgggca
     1021 tcatcaatgc cggcgggttt ttgctgaacc ggctcgcccc gctgtatggg ctcagccccg
     1081 gcaccttgca cgtggtgttc gcggtggggt tgctcaccgc cttgctcggg gcgggcatga
     1141 tgctgaccca gaacgacatc aagaagaccc tgggctactc cacgatcggg cagatgggct
     1201 acatgatcat ggagtgcggt ctgggggcgt tcgccctggc ggtctttcac ttgatcgcgc
     1261 acgggttgtt caaggggacg atcttcttga attgcggcaa cgtgatccac gcggcccgcc
     1321 aggagcccag acttccgcat caacgtgcca cggacgaaca gacggacttt tcgctcctga
     1381 cctgggtcac cggatttgcc gccaccctga tcctgcccct gatcattctg ctggccgcgc
     1441 acggcgtgtt ggaccttacg tttcgggacg cgcaggggat ggtgatcttc ctattcttcg
     1501 cctgggtcac gtcctcccag gccatcttga ccttgtaccg gttgcgggcg gtcaactccc
     1561 ccaaggtggc cgcactcatg ctcgtcacgt tgctgctggt ggttgtgacc tacgtgttgg
     1621 cgggcgagcg attcacctat ttcctgtatc cgaacgcggg cgaggtcgcc gggtactttc
     1681 actccgcggc gttgccggga tggctgttcg atctcatcgt ggcggtcacc gcgttgttcg
     1741 tgatcatggg ctggacgctc atgtacgcgc ggagtcatgg gcgggtgatt cggatcccgg
     1801 actggataga cacggtgcgc gtgcgcgtgt acctgcttct catcaaccga ctctacgtcg
     1861 acgccttgtc cttgcgggtc ggccgccggg tgagatccat cgcccatcgg gtggatcgga
     1921 acgcgctcac cccggtggtg gtgggtgtgc tggccctggg agcgagcgcg cccgcgcttg
     1981 cgatggcggc tgacttgccg gtcgggacgg tcgtgggcct ggtgctcgcg gccatcctgc
     2041 tcccgctctt tcccctgcac ggggcctacg tggcggcgct gacccgcgtg ccggggccca
     2101 tcgccgtggg cctcgcgttc gtgctgccca tcgcggggct ggcattggtc atgaatctgc
     2161 tccccgcgat ccccgcggag ctgctcggcg gggtgggcgt gttggccgtc gtcggtgcgc
     2221 tctacgggtc gttcaaggcc ctggtgcaag cacgcgtgat gcatctgttg gcctacgccg
     2281 gtctggccct gctgtcgatc gcctggtggc atctggccgc ggtcggacgc ccgacgaacc
     2341 gggccgtcgc ctttgtctgc gccgtcggac tggtcatcgg cgggatgtgc ttcgcgtggc
     2401 agcgcgtacg ggcgcgatac ggcgagcttg atccggatcg gatcagtggg ctagcgcaac
     2461 ccatgccccg attcgccgtg ttgctggcgt tgctggtgat ggcgggcgtg ggtctgccgc
     2521 cgttcggtct ctttgccggg ttcttcgcga tggtggtgga cccctccgtc gcgggcacgt
     2581 ggggcctggg tgcaatgctc gccacgtggc tggccgcgtc gtggtacctc ttcaaactga
     2641 tccagcgact cctgttcggc ccgcaccgcg cggacatcct gtacgaggat ttccgcgggg
     2701 cggaagtcgc ctcgctcgcg ctcatcgtgc tggtgttggc ggcgttgggg gttgcgccgt
     2761 acgcgttcct tgacgccgac atgctggccg gcctcgcgtt ctcagccctg gacctgtcca
     2821 cgtggaaccc gtgaccgccg cgtacagcga cacgcagcgc atgcagcttc gggcgctgat
     2881 cctgctcgcc agcgagatcg tcgcctcgta ttggccgatg cggatcttcg tccatcacaa
     2941 cccgttgcac gggctggagg atctcccttt cgacgaggcg gtgcgccgcg cgcatcggtt
     3001 tttgggcggc aaagggtact tgtccaacgc gcgctttcgc gactacgcgg gctcgggccg
     3061 gattcgttcc cgtcacatcg acgccgcgct caaaccgctc gcgcgcgata aacgcgtgac
     3121 gatcggaggg cgcgaggtca cgcacctcga cgcgctgcgg gcgcacctgc tccacgggat
     3181 cgcggagccg gaccgcgaca ccgtcgagcc tctgatcgat cgccgcacgg atcgcgcgat
     3241 gatcgccgcc ttggcggctc ggctcggccc ggtactgcac gcgtcgcgcg tggcggaccg
     3301 ccaggagtcc gaggcggcgc aggccggggc cgcgctggtt cggcgttcca cattggccgc
     3361 gtggtgcgat cgcgtcctgc gcacccagct caccgagcgg atcaaccgcg agatgatcaa
     3421 gtggtgcgaa gcctttctgg acgaagacca cgcggcctgg gccatgccgg gccgcgagca
     3481 gagtttctac ggcgcgtggc gggtgttggc ccagcaagaa tggtcgccct gcgggatcgc
     3541 ggacagccgg cggaaaatcg cccgcttgcc cccggcgcct gaggacgcgc tgctgcagca
     3601 ccttgccgcg ctcggcatcc cgtcgcaggg gcggcaggag tatctgaccc tccacctgac
     3661 cgcgttgccg gggtgggccg gcttcatcaa gtggcgcgcg gatcagagcg agagcgcctg
     3721 gcaacagacg tttcccgctg atctggtcca gtacctggcg gtgcggctgt ggtacgaacg
     3781 ggaattggtc caggaggcgt gccgggaagg tctggggatc gacggaacgg tgagcgcgat
     3841 tgcggccgct gtatcgaccc gcgatgacgc gcaaacaccg gggcttgacc aggacgccgc
     3901 gatgcggggg gccgcggcgc tccggctact ggctctagcc gcgccgcttg cgatcgatcc
     3961 cgcgacgatg atcgcggcgc cgccggagga tctcaaggcg ctgctggact ggatcgacgg
     4021 atttcccgtg tccgagcacg gcccggtctg gctcaatgcg ttcgaggccg ggtaccggga
     4081 ccagttgatc gagactctgc ggcgcgggcg ctccaattcg gaagcgggat cggacgcgga
     4141 gcagccggcg gttcgcccgc aggcccaagc ggtcttctgc atcgacgtgc ggtccgagtc
     4201 gttccgtcgc cacctcgagg cggtggggga tttcggcacg tacggctttg ccgggttttt
     4261 ctccgtgttc atccggtacc gcgcgttggg cgctcatcac gacaccgatc agttcccggt
     4321 gatcatgaag gccaagaaca cggtgcgcga agtgccgcgc acgtatcaag gggcgttgct
     4381 gtcccggcgc caggccggcg ccaggatgct cgacgcggga cactcgctgc tgcacgacct
     4441 caaggaaaac gtggtcacgc cctacgtcat ggtcgagtcg ctcggctggt tctacagcct
     4501 gcccatgatc ggcaagacgg tcttcacctc ttggtaccgg acttggaccg cctggctgcg
     4561 gcgcaaggtc gtgcccgtga tctccaccgc gatgaccgtg gacaaattgt cgcgggagga
     4621 agtggatgag atgctggccg cggagcagcg gtcggtgatt cgccgggcct tgcaggagcg
     4681 gttcggcgac cgcgatctga acgtgtcgct cgagcgattg gagggcctgc ggcgtcgggc
     4741 cttggacgag caggccgtgc ccgcaacgtc cccggaggct cgccgcgggt acgcgctctc
     4801 cccggaggag gaaacctggt tcgtggacgt gctgcgccga gactaccgga tcaaccaggg
     4861 cggagcgttc gcgcgcatgg agcgcatcac gcagatcggg ttcacgctcg cggaacaggt
     4921 ctttaccgtg gaaaccgcgc tgcgcgtgat gggactgacc aagaactttg cccgcctggt
     4981 gctgtgttgc ggccacggga gcacctcgga caacaatccc ttcgaggccg cgctcgattg
     5041 cggcgcgtgc ggcggcaacc cgggcgagcc caacgcgcgg gtgttggcgg ccatggccaa
     5101 caaagcccaa gttcgggacg cattggcgaa agccggcatc gtgattcccc aggacaccta
     5161 ctttatcgcg ggccggcacg acaccaccac ggacgaggtg cggctgttcg acctggagga
     5221 tctcccgcac acgcatcgca acgacatgct ccggctggaa gccgcgcttt ttgaggccgg
     5281 gcttcgcaac aatcgcgagc ggtgcgcccg attcccggaa atgccagagc ccttgaccac
     5341 gaagcgcgcg gccaaggagg tgcgccggcg cggcagcgac tggagccagg tccggccgga
     5401 gtggggcttg tccggcaacg cggcctttat catcgggcgg cgcgatctga cgcgcgggtt
     5461 gccgctcgac gcccgggtgt tcctgcattc gtacgactac cagggcgacc cgaccggccg
     5521 gctgctggaa atcgtgatga ccgggccgca ggtggtgggg cagtggatca acatggaaca
     5581 ctacttctcc accgtggatc ccgaagtcta cggcagcggc agcaagatct accacaacgt
     5641 ggtgggccgg ttcggcgtca tgtccggacc ccagagcgac ctgcggaccg gcttggccgc
     5701 gcaaaccgtg atggacggac cgcggccgta tcacgaaccc atgcgcctgc tcaccctgat
     5761 cgaagcgccc accgaccgca ttacccaggt catccgccgc caccgcgtgc tgcagcggct
     5821 ctacgacaac gaatgggtgc gcctcgcggc gcttgacccg gacacgcgaa ttttctatta
     5881 ctatgtcccg aggcagggct ggaccgtgat gaacgacggc ggcccgtctc cccacacgca
     5941 cgcgtcgtga tcgagaa
//
LOCUS       DBNGTT010000306         9165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3388583, whole genome shotgun sequence.
ACCESSION   DBNGTT010000306 DBNGTT010000000
VERSION     DBNGTT010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3388583"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="AC16BC_06815"
     CDS             <1..419
                     /locus_tag="AC16BC_06815"
                     /inference="COORDINATES: protein motif:HMM:NF019268.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3241369.1"
                     /translation="LIDIVATLKVVDGLTLVFNLDRGSQSAPFGAGTGTALWQGYAIY
                     ANMALGDKHSVTVRGEVFDDQEGFKSGATFQPEGVTGRELTITFSCKMKENLEWRAEV
                     RHDEANRDVFAGSDAAATNPDKDSQNTVAVAAYYSF"
     gene            565..903
                     /locus_tag="AC16BC_06820"
     CDS             565..903
                     /locus_tag="AC16BC_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3241370.1"
                     /translation="MKKIEAIIKPFKLDEVKEALTEIGVQGMTVTEVKGFGRQKGHKE
                     VYRGAEYVVEFVPKVKIEIVVADALSQRAVEAIVRAAKTGTIGDGKIFVTSLSEAVRI
                     RTGETGEGAL"
     gene            complement(1066..2121)
                     /locus_tag="AC16BC_06825"
     CDS             complement(1066..2121)
                     /locus_tag="AC16BC_06825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR3241371.1"
                     /translation="MKELQALLDISKSLPTTQSRQEVLYLLVKAIAGAVDVTRCSVIL
                     VDEESSKASVLATFEDSALREIPIDLHKYPEIQEAVATASMVLVEDVERDARMQEVAP
                     LFRHLQIASILVIPIMFYERVLGTLFLRTSRPHSAFTREEILFCETASYMAANFLLGM
                     TQYQFAVEEKARLAEEAMRDPLTGLGNRSSFSRALSESIAYARRHQRPLACLMIDADN
                     FKSINDRFGHHQGDHVLRGVADAITNALRESDLAARYGGDEFAVLLRDTDGPGAFIKA
                     ERIRDAVRYSRLVDGADPVTVSIGIAVWNDLIGLDPDYDLVAEADRALYAAKGHGKNR
                     TVPTFSEPSRPAQTDAA"
     gene            2282..3196
                     /locus_tag="AC16BC_06830"
     CDS             2282..3196
                     /locus_tag="AC16BC_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HIR3241372.1"
                     /translation="MCGWFGLAAFAAAPSLAAADAVVSTIHGPFAFRATALDQRLVNA
                     LQPLADRALDRLTSDLGGSSVGPITVVLAPDDRAFNQFQSPGAPLPEWATGVAYPRTM
                     TMVIRSYHVTGTPRQDIGTIFVHELTHLLLGARFGDRPVPAWLHEGLAMYEAGEWRFE
                     HEWDLVWAVLGNNVPRLDTVTESFPVSEAEARIAYLLSEALVGYMITTYGRDGFSAFV
                     DRLAEGQSLEPALIRAFGVTQSRFEERWRAHLDRRYAWIPLITSSSAVWVIMMAVAFA
                     AYAARRRRNRKIVEAWEEEERRPEQGPP"
     gene            3376..4668
                     /locus_tag="AC16BC_06835"
     CDS             3376..4668
                     /locus_tag="AC16BC_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241373.1"
                     /translation="MRPSLGVSSRYRIGEAVAVVGISLTFFAVLLAFLRSIFPAGAGL
                     REGVTPEGLLPVAAVTEHTGRDLLLTSGSKEARLGTEPREAAAAVLVRMINTVKHRPV
                     DAISWSAGRQGERLRDGDAVQTFDNSTARIRFAEGNVLDMSANSLVVIQRTERDPVRS
                     EMHSQLVMVGGELRGTVGAGGASAMEVALPGGVVKIRPGSRDRADAQFKISTSKQRGS
                     SIAVYRGVAELDANGRTLHVAPNFASTLSPDGAMAKPVPLPARPVLAGSVADRTENIR
                     FSWKGPASVDAYHVVIARDPGFDHVVYDAHVVTPELRYGDLQRGTYYWRVSGIAGGVE
                     GAYSEPGRVTVGQDREPPALEVHVSQKARGSHTFTITGRTEPGAQVFAGGRHIPVTDA
                     GSFQFTMELDPGSNVVAIRAEDQAGNVTYRSELVSAEF"
     gene            4668..6593
                     /locus_tag="AC16BC_06840"
     CDS             4668..6593
                     /locus_tag="AC16BC_06840"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3241374.1"
                     /translation="MALVSALLRVRMPLRVKFLIALLFLVTSVMGAITFTMANLFHTD
                     KKTYIHDLTSIISLHTAQETRSLLVGYQERINLFARLMGDRSLAHENKARLLKSLFQE
                     FPEVVAMSLYTNEREPVTAYDAAQLSAAGLTKQDFLALRREQPIPVDRVRSGAVFVEN
                     STLTAKLPMLTMAVPQPVADGGSAVVAAAVIRLDGLLRVARRSSAFETFVVDGRGAVL
                     AHTDPELVVRRAPVGWIPESALEWLHSPEHAGGAETASVTREYAHEGVEMVGGFARVD
                     FGGLLAGVQIPKAAAYLTARELLNSLIGVAFLLLAVAAVAGLLWSRLITRSIEGLSAA
                     TRVVAKGDFDIQLAHGSSDEIGSLAESFNQMASELKVREHALKQAQAALIQSEKMAAF
                     GQLGAGIAHEVKNPLAGILGYAQLALRKIGKDDATHQHLQIIEKEAKRCKTIIESLLR
                     FARQEPTEYAPVDLNRVVDEGIAIVDHQLTIHRVTLEKRLSPVPVIMGSGNQLQQVLM
                     NLLINAQQAMEPDGGTVRVSTGVAGGVVELRVSDTGPGVAPEVRSKIFEPFFTTKPAG
                     KGTGLGLSVTYGIVHDHRGEIRLADEPGRGATFVVTFPVAESPGSVRDGASGGAAEQD
                     DNRDAPNGHDPLHRQAA"
     gene            6629..7597
                     /locus_tag="AC16BC_06845"
     CDS             6629..7597
                     /locus_tag="AC16BC_06845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR3241375.1"
                     /translation="MFRVLVVDDESSLRGILSELLGDAGYYVETAESGEAALERIKNS
                     AFHVVITDIRLGGMNGIELIRLIRGMGVETEVIVMTSHASFSTAVEALRLGAYDYLMK
                     PFEDLDAVLALVKRTIDKIRLVLENRRLVETLKRKNEELEQLAVRDGLTGLYNYRYLQ
                     ESLQTEVNRSERHGYLLCLMMVDVDHFKQYNDHNGHLDGDEVLKIVARILGERARRSD
                     TVARYGGEEFCVILPETRLDLAAKVAEDVRHLVENYPFQNRDKQPLGRVTISLGVAEF
                     PADASSARTLIDRADQALYRAKTEGRNRVRLYRDAAESPGASPRAA"
     gene            7991..8452
                     /locus_tag="AC16BC_06850"
     CDS             7991..8452
                     /locus_tag="AC16BC_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HIR3241376.1"
                     /translation="MGRRDKVKRGLGRKSPKSDFTSSSGGETDVAPLGRAGAKPAHER
                     TADEDVDVVDWDRVAGREDFRRLVAAKRAFIVPATVFFIAYYFALPLLVGYAPDLMKT
                     EAFGAVNLAYLFALSQFVMAWGLAAWYLRAAGRFDGMAHAILGKLVRRKAG"
     gene            8452..>9165
                     /locus_tag="AC16BC_06855"
     CDS             8452..>9165
                     /locus_tag="AC16BC_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute symporter family protein"
                     /protein_id="HIR3241377.1"
                     /translation="MSTSLLMFLSFIALTLWVTYWAAGRSGDSSAFFAANRRITGWQN
                     GVAVAGDYMSAASFLGIAGIIAFQGYDGFMFSVGWLVAYLTVLLIVAEPLRNAGKYTM
                     ADVLAYRLSPRPVRAMASLSTLTVSTFYMIAQMVGAGALVALLLKDSGLSFEAAVIGV
                     GVLMIVYVVFGGMLATTWVQIIKAALLMGGTVLLSVLVLVHYGFDLGDLFRAASHGPA
                     HQQGRLDDLHPRGRQHAAEH"
BASE COUNT         1590 a         2707 c         3162 g         1706 t
ORIGIN      
        1 cgttgatcga catcgtcgcc accttaaaag tggtggatgg cctcaccctt gtgtttaacc
       61 ttgaccgtgg atcccagtca gcgccattcg gtgccggaac cggtacggct ttgtggcagg
      121 gatatgccat ctacgccaac atggcattgg gtgataagca ttcggtcacg gtccgtggcg
      181 aagtgttcga tgaccaggag ggcttcaaat ctggggcaac cttccaaccc gaaggtgtga
      241 ccggtcgtga actaacgatc accttctcct gcaagatgaa ggagaacttg gagtggcgtg
      301 cggaagtgcg gcacgacgag gccaatcgag acgtgttcgc cggcagcgat gccgcagcga
      361 caaatcctga taaagatagc cagaacacgg ttgcggtcgc ggcgtactac tcgttctaag
      421 gaacgggacg cagggatttg agtcgtgtct gtggcctccg cgccatcctg cttggcggga
      481 tggcgcggga ggccacagcc ggctggttga tcgagccata tggcgcgaga gggcagaacc
      541 tgagcagccc cggaggcgga acggatgaaa aaaattgagg cgatcatcaa gccgttcaag
      601 ctcgacgagg tcaaggaagc gttgaccgag atcggcgtgc agggtatgac cgtgaccgag
      661 gtcaaggggt tcggccggca aaaggggcac aaggaggtct atcgcggcgc ggagtacgtg
      721 gtggagtttg tgcccaaggt caagatcgag atcgtggtcg cggatgcgct gtcgcagcgg
      781 gcggtggagg cgatcgtgcg cgcggccaag accgggacca tcggcgacgg caaaatcttc
      841 gtcacgtcgc tcagtgaagc ggtgcggatt cggaccggag aaaccgggga gggcgcgctc
      901 taggggaggt tgtcgtcatt cccgtgaacg cgggaatcca ggattccttc agtcggtgcg
      961 aacgcttgga aggataggtt tcttccggag gtcggtgtct gatggcgccg gcctcatgtt
     1021 gtttatgggg tcatgtcgga tgttcggcgg gcgagtcgag aagactcaag ccgcgtcggt
     1081 ttgggcgggt cgtgacggtt cgctgaacgt cgggacggtg cggttcttcc catggccttt
     1141 ggccgcgtag agcgcccgat cggcttcggc gaccaggtcg tagtccggat ccagcccgat
     1201 caggtcgttc cagaccgcga tcccgatgct gaccgtgacc gggtccgcgc cgtcgaccag
     1261 acggctgtac ctgaccgcgt cccggatccg ttccgctttg atgaaggccc cgggaccgtc
     1321 cgtgtcccga aggagcaccg cgaattcgtc gccgccgtag cgggccgcga ggtccgactc
     1381 ccggagcgcg ttggtgatcg cgtccgcgac tccgcgcagc acgtggtcgc cctggtgatg
     1441 cccgaatcgg tcgttgatcg acttgaagtt gtccgcgtcg atcatcaggc atgccagcgg
     1501 tcgttggtgc cgtctggcgt aggcgatgga ttcggagagg gcgcgggaaa aactcgagcg
     1561 gttcccgagt ccggtgagcg ggtcccgcat ggcttcctcg gccaaacggg ccttctcctc
     1621 gactgcgaac tggtattggg tcatgcccaa cagaaaattg gcggccatgt acgaggcggt
     1681 ttcgcagaac agaatttcct cgcgcgtgaa cgcggagtgc ggacgcgacg tgcgcaggaa
     1741 gagcgtgccg agcacacgct cgtagaacat gatcggaatg accaggatcg aggcgatctg
     1801 caggtgccga aacaacgggg cgacctcttg cattcgcgcg tcgcgctcaa cgtcctccac
     1861 cagcaccatc gaggccgttg caacggcctc ctgaatttcc gggtacttgt gcaggtcgat
     1921 ggggatctcg cgcagcgcgg agtcttcgaa tgtcgccaac acgctggcct tcgaggattc
     1981 ttcgtcgacc aggatcaccg agcaccgcgt cacgtccacc gcgccggcaa tggctttgac
     2041 cagcaaatag agcacttcct gccgactctg cgtcgtgggc aggctcttac tgatatcgag
     2101 gagggcttgg agttctttca tcagtcggtc gcgtcgtgcg atcggcgtga agaactgtag
     2161 cacaggcgcc gaaacccgca agccgtgcgc cggaagcccg cctcatccgg cggtattggc
     2221 gcgaaccgcg gcgccgtcat cctgtgtggc gcgtatgatg gagcgtctcc ttcggtggtc
     2281 cgtgtgtggg tggtttggcc tggcggcgtt cgcggcggcg ccgagtctcg cggccgccga
     2341 tgccgtagtc agcacgatcc acggtccctt cgcattccgc gcgaccgccc tcgaccagcg
     2401 attggtgaac gcgctccagc cgctggcgga tcgcgcgttg gaccggctca cgtccgacct
     2461 cggaggatcg tcggttgggc cgatcacggt ggtcttggcg ccggacgatc gcgcgttcaa
     2521 ccagttccag tccccgggcg cgccgctgcc ggagtgggcg acgggagtag cctatccccg
     2581 caccatgacc atggtcattc gttcgtatca cgtgacgggg acgccgcgac aggacatcgg
     2641 cacgattttc gtccacgaac tgacgcacct gctgctgggc gcgcgtttcg gcgaccggcc
     2701 cgttcccgcg tggctgcacg aaggcctggc catgtacgag gcgggcgagt ggcggttcga
     2761 gcacgaatgg gatctggtct gggcggtgct cggcaacaac gtcccgcggc tggataccgt
     2821 gaccgagtcg tttccggtga gcgaggccga agcgcggatc gcgtaccttc tgagcgaagc
     2881 gctggtgggt tatatgatca cgacctacgg ccgcgacggg ttcagcgcat tcgtggaccg
     2941 attggccgaa ggacagtccc tggagccggc gttgatccgc gcgttcgggg tcacccaatc
     3001 gcgcttcgag gagcgatgga gagcgcatct ggaccggcgc tatgcctgga ttcccctcat
     3061 cacgagttcg tcggccgtgt gggtgatcat gatggccgtg gcgttcgcgg catacgcggc
     3121 gcggaggcgg cgcaatcgga agattgtgga ggcttgggag gaggaagaac gacgacctga
     3181 acagggccct ccgtaaacgg gtgcccccga cgacggaccg cgaagccgtc tcgaccgacg
     3241 ggtgttcccc tcgaaacact cagccgtttc cccagacccc aattcactgg ggacggtctg
     3301 gcgttcatgg gcgcgggagg tccgtaaact tcgtgcggtt tcagcgggtt acgctacact
     3361 gtagcgcgcg tggcaatgag gccttcattg ggcgtgtcca gtcggtaccg catcggcgag
     3421 gcggtggcgg tggtcgggat ctcgttgacg tttttcgccg tgctgctcgc gtttctgcga
     3481 agcatcttcc ctgccggggc cgggttgcgc gaaggggtga cgccggaggg attgcttccg
     3541 gtcgctgccg tcacggagca cacggggcgg gatctgttgc tgacgtcggg atccaaggag
     3601 gcgcggctcg ggaccgagcc ccgagaggcc gctgcggcgg tgctcgtgcg gatgatcaat
     3661 acggtgaaac atcggcccgt cgacgcgatt tcgtggtcgg ctggtcgtca aggcgagcgt
     3721 ctccgcgacg gcgacgccgt gcaaacgttc gacaattcaa ccgctcgtat ccgattcgcc
     3781 gagggcaatg tcctcgacat gagcgccaac tcgctggtgg tgattcagcg cacggagcgc
     3841 gatccggttc ggagcgagat gcactcgcaa ctggtcatgg tgggtggaga gcttcgcggg
     3901 accgtcggcg ccggcggcgc gtcggccatg gaggtcgcgc ttccgggcgg cgtggtcaag
     3961 attcggccgg gatcccgaga ccgcgccgac gcccaattca agatcagcac gagcaaacag
     4021 cgcgggtcca gcatcgcggt ctatcgcggc gtggcggagc ttgacgcgaa cgggcgaacg
     4081 ctgcacgtcg ccccgaactt tgcgtcgacc cttagtccgg acggcgcgat ggcgaagccg
     4141 gtgcctctgc cggcgcgacc ggtgctcgcg gggtcggtgg cggaccggac ggagaacatc
     4201 cgattctcgt ggaaggggcc ggcatccgtg gacgcatatc acgtggtcat cgcccgagac
     4261 cccgggtttg accacgtggt ctatgacgcg catgtcgtca cccccgaact tcggtacgga
     4321 gaccttcagc gcggaacgta ttattggcgg gtgagcggca tcgcgggcgg cgtcgagggc
     4381 gcgtacagcg agcccgggcg cgtgaccgtc ggtcaggacc gcgaaccgcc ggcgctggaa
     4441 gttcacgtgt cgcagaaagc cagggggtcg catacgttta cgatcacggg ccgtaccgag
     4501 ccgggggcgc aggtcttcgc cggaggcagg catattccgg tcacggatgc agggtcgttc
     4561 cagttcacca tggaactgga tccgggatcg aacgtggtcg cgattcgggc cgaagatcag
     4621 gcggggaatg tcacctatcg ctcggaactc gtgagcgcgg agttctgatg gcgctggtct
     4681 cggcgttgct ccgcgttcgg atgccgctca gggttaagtt cctgatcgcg ctgttgttcc
     4741 tggtgacgag cgtgatgggc gccattacct ttacgatggc gaacctgttc cataccgaca
     4801 agaagaccta tatccacgac ctcacgtcga tcatctcgct gcacaccgcc caggaaacgc
     4861 ggtccctgct cgtcgggtat caggagcgga tcaacctgtt cgcgcggctg atgggggacc
     4921 ggagcctggc gcacgagaac aaggcgcgcc tgctcaagtc cttgttccag gagttccccg
     4981 aagtggtggc gatgtcgctc tacacgaatg agcgcgaacc cgtgaccgcg tacgacgcgg
     5041 cgcaactcag cgccgccgga ttgaccaagc aggattttct ggcgctgcga cgcgaacaac
     5101 cgattccggt cgaccgcgtg cgatcgggcg cggtgttcgt cgaaaactcg acgctgaccg
     5161 caaaacttcc gatgctgacc atggccgtgc cccagcccgt tgccgacggc ggaagcgcgg
     5221 tcgtcgcggc cgcggtgatc cgtcttgacg gcctgttgcg ggtcgcgcgt cggtcgtcgg
     5281 cgttcgaaac cttcgtcgtc gacggccgcg gggcggtcct tgcgcatacc gatcccgagt
     5341 tggtagtgcg tcgcgcgccg gtgggctgga ttccggagtc cgccttggag tggctccaca
     5401 gtccggagca tgccggcggg gcggagaccg cgagcgtcac gagggagtac gcccatgagg
     5461 gggtggagat ggtcggcggg ttcgcccggg ttgacttcgg gggactgctg gccggcgtgc
     5521 agattcccaa ggcggccgca tatctgacgg cgcgtgagtt gctgaatagt ctgatcgggg
     5581 tggcgtttct actgctcgcg gtcgcggcgg tggcggggct cttgtggtcg cgactgatta
     5641 ccaggtcgat cgaaggcctc tcggcggcga cgcgcgtggt ggcaaaggga gacttcgaca
     5701 tccaactggc gcacggttcc agcgacgaga tcggcagtct cgcggaatcg ttcaaccaga
     5761 tggcgtcgga gctcaaggtg cgggagcacg ccctcaagca ggcccaagcc gcattgatcc
     5821 agtccgagaa gatggcggcg ttcggccaac tgggtgcggg cattgctcac gaagtgaaaa
     5881 acccgctcgc cgggattctg gggtatgcgc aactcgcgct gcggaaaatc gggaaagacg
     5941 acgccacgca tcagcattta cagatcatcg agaaagaggc caagcgctgc aagacgatca
     6001 tcgagagcct gttgcgcttc gcgcggcaag agccgacgga gtacgcgccg gtcgacctca
     6061 accgggtcgt cgacgaggga atcgcgatcg ttgaccatca gttgacgatc catcgagtca
     6121 cgcttgagaa gcgcttgtct ccggtcccgg tgatcatggg gtccggaaat caactccagc
     6181 aggtgctgat gaatctgctc atcaatgccc agcaagccat ggagcccgac ggcggaaccg
     6241 tccgcgtgag caccggcgtt gccggcggcg tcgtggagct tcgcgtctcg gacacgggac
     6301 cgggggttgc gccggaggtc cgctcgaaga tcttcgagcc gtttttcacg acgaaacccg
     6361 ccgggaaggg aaccggcctc ggattgtcgg tgacctacgg gatcgtccat gatcatcggg
     6421 gtgagattcg gctcgccgac gaaccggggc ggggcgcgac ctttgtcgtg acgtttcccg
     6481 ttgcggaatc ccccgggtcg gtaagagacg gagcctcggg aggcgcggcc gagcaggacg
     6541 acaaccgcga cgcgccgaac ggtcatgatc cacttcatcg gcaggccgcg tgatgaacga
     6601 tacggcgagc ggtcgggaag aggtatccat gtttcgagtc cttgtcgtcg acgatgagtc
     6661 cagcctgcgt ggaatcctca gcgaattgct gggagatgcc gggtattacg tggagacggc
     6721 cgagagcggc gaggcggcgc tggagcggat caagaattcg gcgttccacg tggtgatcac
     6781 ggacattcgg cttggcggga tgaacggtat cgaactcatt cgcctgattc gcggcatggg
     6841 cgtggagacc gaagtcatcg tcatgaccag ccacgcctcg ttctcaacgg ccgttgaggc
     6901 ccttcggctg ggcgcgtacg actacctcat gaaaccgttc gaggacctgg acgcggtgct
     6961 ggcgctggtg aagcgaacga tcgataagat tcgcctcgtg ctggaaaatc gacgtctggt
     7021 cgaaaccttg aaacgaaaga acgaagaact cgaacaattg gcggtccgcg acggcctcac
     7081 cggtctctac aattatcgct atctccagga atcgcttcag acggaggtga accggtccga
     7141 gcggcacggc tatttgctct gcctgatgat ggtggacgtg gaccacttca aacagtacaa
     7201 cgaccacaac ggccacctgg acggcgacga ggtgctcaag atcgtcgccc gcatcctcgg
     7261 cgaacgcgcc cggcgctccg ataccgtcgc gagatacggg ggagaggaat tctgcgtgat
     7321 tctgccggag acgcgtttgg acctcgcggc aaaggttgcc gaggatgtcc gccatctggt
     7381 cgagaactat ccgttccaga accgcgacaa acaaccgctc ggacgagtga cgatcagtct
     7441 cggcgtcgcc gagttcccgg ccgacgcgtc ctccgcgcgg acgttgatcg atcgcgccga
     7501 tcaagccttg tatcgagcca agacggaggg gcgaaatcgc gtccggctat accgggatgc
     7561 cgcggaatcg cccggtgcgt cgcctcgcgc cgcgtagatg tcttaccgag ggcttctttc
     7621 gtccgtccgc gatccgcgtg gcgtgcagcg acctgcaccg ggctgtttgg gagcacactg
     7681 cgcgccggta tcgatccacg ttactgccga gtgaagcgca acgggtggcg aggctcatcg
     7741 gcgcccgcca gatgctctcg accgccattg aagcggacct ttggcgtttc aacgcctgat
     7801 caacgcaagg taatcgcgac tctctcacct gggggcctcg ggaccgacga ctgcgcctgc
     7861 gctgacgtga ctggtcgtgg cgaacctgcc gcgatcgcgg tctcgacggc atcggtgatt
     7921 gaccgcgtga tttctgctac actgcccgcc gaacagctga cgatcgggtc cagacgcgcg
     7981 tgagcggggc atgggacgac gggacaaagt caaacgcggc cttgggcgaa aaagcccgaa
     8041 gagtgatttc acgtcgtcga gcggtgggga aaccgacgtc gcaccgctcg gacgcgcggg
     8101 cgccaaaccc gcccacgaac gaacggcgga tgaagacgtc gatgtcgtgg attgggaccg
     8161 cgtggccggg cgcgaggatt tccggaggct cgtcgcggcg aagcgcgcgt ttattgttcc
     8221 ggccacggtg ttcttcatcg cctactactt cgcgctcccg ctcctggtcg gctatgcgcc
     8281 cgacctcatg aaaaccgagg ccttcggcgc ggtgaacctg gcctacctgt tcgccttgtc
     8341 ccagttcgtg atggcgtggg gcctggccgc ctggtatctg cgcgcggcgg gtcgcttcga
     8401 cggcatggcg cacgcgatcc tcggcaagct tgttcgccgg aaggcggggt gatgtcgacg
     8461 tcgctcctca tgtttctgag ttttatcgcc ctcacgctct gggtgaccta ttgggcggcg
     8521 gggcgttcgg gcgacagcag cgcgtttttt gccgcgaatc gacggatcac cgggtggcag
     8581 aacggcgtgg ccgtggccgg ggattatatg agcgcggcgt cgttcctcgg catcgcgggg
     8641 atcatcgcgt ttcaggggta cgacgggttc atgttctcgg tcggctggct ggtcgcgtat
     8701 ctgaccgtgt tgctcatcgt ggccgaaccc ttgcgcaacg ccggtaagta caccatggcg
     8761 gacgtgctcg cgtaccggtt gagccccagg cccgtgcgag cgatggcctc gctctccacg
     8821 ctgaccgtca gcaccttcta catgatcgcg cagatggtgg gcgccggcgc cttggtggcg
     8881 ctgctgctca aggactccgg tctcagcttc gaggcggcgg tgatcggcgt gggcgtgttg
     8941 atgatcgtgt acgtggtgtt cggcggcatg ttggcgacca cgtgggtgca gatcatcaag
     9001 gcggccctgt tgatgggcgg gaccgtgctg ctgagcgtct tggtgctggt gcactacggt
     9061 ttcgatcttg gcgatctctt ccgcgccgcg agccacggtc ccgcccatca acagggccgc
     9121 cttgatgatc tgcacccacg tggtcgccaa catgccgccg aacac
//
LOCUS       DBNGTT010000307         2379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2366997, whole genome shotgun sequence.
ACCESSION   DBNGTT010000307 DBNGTT010000000
VERSION     DBNGTT010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2366997"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16BC_06860"
     CDS             <1..237
                     /locus_tag="AC16BC_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3241378.1"
                     /translation="DEAALVEALRLGRIAGAGLDVYEREPEIQAGLDACDNTLLVPHL
                     GSATTATRERMGAMVVENVLAVLEGRRPPNPVEA"
     gene            complement(283..1617)
                     /locus_tag="AC16BC_06865"
     CDS             complement(283..1617)
                     /locus_tag="AC16BC_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3241379.1"
                     /translation="MAVSTMKDQTIEVGEERAVATPGEEIERGELDGDDMSRDLARVS
                     VEIASPEAEPEEAERETEAEQEATEDLIWVYFRTMGKKELLTKEAETKLFQRLELGLQ
                     MLADVIRSVRPKDRTALDRLQRFFTDEDQARLTSVTNRLSAAVPEEQRPLVALIRTLV
                     ESATTKEAPDPANRERWERRFRGEGLDLKTVVRWNTQLAEGEAIITEATRELVEANLK
                     LVISIAKRYMGRGLALLDLIQEGNMGLMRAITRFEYTKGFKFSTYATWWIRQGITRAL
                     ADQSRTIRIPVHLTEAFHKILTAHQTLKQELSRAPNSKEIARRTGIPAKKVDEILRVV
                     QEPLGLQDPVGGEELQLQDLIRDEISPSPYDRLLTSEVSQRVADILDTLTPKESLVIK
                     MRFGIGGAKESTLEEIGSIMGITRERVRQIESQALRKLRYPDRLKKLQTLRG"
     gene            complement(1791..>2379)
                     /gene="epsC"
                     /locus_tag="AC16BC_06870"
     CDS             complement(1791..>2379)
                     /gene="epsC"
                     /locus_tag="AC16BC_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HIR3241380.1"
                     /translation="FERDPAATGAFEVLLTYAGFHAIVLHRVSHGLHRAGVPMIPRVI
                     SQLGRFLTGVEIHPAARIGPGFFIDHGMGVVIGETAEIGESVILFQGVTLGGTGKERG
                     KRHPTLGNHVVVGAGAKVLGPITIGDHVKIGANSVVLRSVPAHSTEVGIPGKIIKSEA
                     DAPEKSLNHTQLPDPVAERLEALENEVREIKKRLS"
BASE COUNT          425 a          807 c          670 g          477 t
ORIGIN      
        1 gacgaggccg ccctcgtcga ggcgctccgg ctgggccgaa tcgcgggagc cgggctcgat
       61 gtctacgaac gcgagccgga gattcaggcc gggttggacg cgtgcgacaa taccctcctc
      121 gtgccgcacc tcggatccgc gacgacggcg actcgtgaac ggatgggagc catggtggtc
      181 gagaacgtgc tcgcggtgct cgaggggcgc cgcccgccca accccgttga ggcataaaaa
      241 aacggggcgc actgacgtgc gccccgttcg tcccgaagga ccttatcccc gcagggtctg
      301 aagctttttc aaccgatccg gataccgcag cttccggagc gcctggcttt cgatctggcg
      361 cacccgttcc cgagtgatgc ccatgatcga cccaatctcc tcgagcgtcg attccttggc
      421 gccgccgatg ccgaaccgca tcttgatcac caacgattcc ttcggcgtca acgtgtcgag
      481 gatgtccgcc acgcgctgag acacctcgct ggtcaagaga cggtcgtagg gcgacggtga
      541 gatctcgtcg cggatcaaat cctggagctg caactcctcg ccgccgaccg ggtcctgcaa
      601 tcccagcggc tcctgcacca cgcgcaggat ctcatcgacc ttcttggccg gaatgcccgt
      661 gcgccgcgcg atctccttgg agttcggggc gcgcgacaac tcctgcttga gcgtctggtg
      721 cgcggtcaag atcttgtgaa acgcctcggt caagtgcacc gggatccgga tcgtgcgcga
      781 ctggtcggcc aatgcccgcg taatgccttg gcgaatccac cacgtcgcgt acgtgctgaa
      841 cttgaacccc ttggtgtact caaaccgcgt gatcgcgcgc atgagcccca tgttgccttc
      901 ctggatcagg tccagcaaag caagcccgcg gcccatatac cgcttggcga tgctgatcac
      961 caacttgaga ttggcttcga ccaactcccg ggtcgcctcg gtgatgatcg cctcgccttc
     1021 cgccaactga gtattccagc gaacaaccgt cttcagatcg agcccttccc ctcggaatcg
     1081 acgttcccat cgctcgcggt tcgccgggtc cggagcctct ttggtcgtgg ccgattccac
     1141 cagcgtccga atcaaggcca ccaaggggcg ctgctcctcg ggcaccgcgg cggagagccg
     1201 attcgtcacg ctggtcaaac gggcctgatc ctcgtccgtg aaaaaccgct gcagccggtc
     1261 cagcgcggtg cgatccttgg gccgcaccga ccggatcacg tccgccagca tctgcaaccc
     1321 caactccaag cgttggaaga gtttggtctc ggcttccttg gtcaagagtt ccttcttgcc
     1381 catggtccgg aagtagaccc aaatcaagtc ctcggtcgct tcctgctccg cctcggtttc
     1441 ccgctcagcc tcttcgggct cggcttccgg cgacgcaatc tccaccgaca cgcgcgccaa
     1501 gtctcgggac atatcatcgc catcgagttc accgcgctcg atctcctcgc cgggcgtagc
     1561 caccgcacgc tcttctccga cttcgattgt ttgatccttc atggtcgaaa ctgccattct
     1621 gctctcctgt tcacgccgcg aaatacacca ctcgttcgaa tcttcctgat ccgaccgtcg
     1681 ccggtgttta accctgtata aagaacagtt cggaagggct ccattctaac aggagcaagg
     1741 gtcacatgca agttattgct gtttaactaa ttaaatcaat tagttaatag ctacgacaat
     1801 cgcttcttga tctcgcgaac ttcattctcc agcgcttcca atctctcggc gacgggatca
     1861 gggagctgag tgtgattgag cgacttctcg ggtgcgtcgg cctcactctt gatgatcttg
     1921 cccgggatgc ccacctcggt tgaatgggcc gggaccgatc gcagcaccac cgaattcgcg
     1981 ccgatcttga cgtgatcgcc gatcgtgatc ggccccagca ccttcgcgcc cgcccccacc
     2041 accacgtgat tccccagcgt gggatggcgc ttgccgcgct ctttgccggt gccgcccagt
     2101 gtcacgcctt gaaacaagat cacgctctcc ccgatctccg cggtctcgcc gatcacgacc
     2161 cccatgccgt ggtcgatgaa gaagccggga ccgatccgcg ccgcggggtg aatttccacg
     2221 ccggtgagaa accgtcccag ttgggaaatc acccgcggga tcatgggcac gcccgcgcgg
     2281 tggagcccgt gcgacacgcg gtgcaatacg atggcgtgaa accccgcgta cgtcagcagg
     2341 acttcgaacg cgccggtcgc ggccggatcg cgttcgaat
//
LOCUS       DBNGTT010000308         5246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3134035, whole genome shotgun sequence.
ACCESSION   DBNGTT010000308 DBNGTT010000000
VERSION     DBNGTT010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3134035"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..387
                     /locus_tag="AC16BC_06875"
     CDS             133..387
                     /locus_tag="AC16BC_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241381.1"
                     /translation="MFTEEKTFLLRFSLEAAFPEDYEGEADQYQWVREWEGGMKADVI
                     KAVFSALRNRPGWSARVRNRGMSEQDEIEIVLSRDAADPG"
     gene            394..1116
                     /locus_tag="AC16BC_06880"
     CDS             394..1116
                     /locus_tag="AC16BC_06880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3241382.1"
                     /translation="MPTTMKSGGRVTGPEIAVLVKSREPTVLRKVAPYLRWVARGGGR
                     PVVVYLGRRVPASASGLLLVGGEDLAPARYGEANRHCERINAPRDAWELDLLPRALDR
                     DVPILAVCRGIQVLAVALGGTLIQDLPIELAARGVRSRVIHRGPRETDSAHRVAIAPR
                     TLLAKMIPARTALVNSHHHQAVRDAPSNTRVSARSSDAVIEAIEHIHNRFVLGVQWHP
                     ERWNRPSSAAIMRGFLEACGGE"
     gene            1167..2087
                     /locus_tag="AC16BC_06885"
     CDS             1167..2087
                     /locus_tag="AC16BC_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006670498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3241383.1"
                     /translation="MFWMPGASARGRSEPLTAHESALADALRRDVTALGGEIGERNFQ
                     QYDALLAAADYIDHALTASGYEVRRQRYEVDGRPFDNLEVERRGASKPDEIVLVGAHY
                     DSVVGAPGANDNGTGVAAALALAEAFAGRTTARTVRFVAFVNEEPPFAHTPSMGSVVY
                     ATRSRQRGERITAMLSLETMGYFADAPGSQQYPSVLRWVYPDTANFITFVSNLSSRGE
                     LKRAIKSFRHVASIPSEGGALPATISGVGWSDHWSFWQAGFPAIMVTDTALFRYPAYH
                     TPNDTPEQVDYERLARVVAGLEHVVADLAA"
     gene            2091..3206
                     /locus_tag="AC16BC_06890"
     CDS             2091..3206
                     /locus_tag="AC16BC_06890"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3241384.1"
                     /translation="MSKRPTGDAARGAEGSPGYVIPQLTFLRFPAIVWIALFHFGASG
                     FPFSVSPFLLKFVGHGYVAVTFFFVLSGFTLAVRYAGRRIEPTRFMLARLARIYPLYL
                     VGLAVCGLIFAYFGTLQRNLPTLPLNVLLIQAWVPSFALTWNKPCWFLSVLLLFYLIF
                     CLSFDRVSRMTPKTMAYSLTVIWLASLAALVLLKATVYTGEPSAIHDVIFYHPIMHLN
                     SFLIGVGGGLAFRADALQRMAGGRPGWVLATGALVALLGIVAVDDSGYVLHDGLLAPL
                     FLVLIVGVALDTSMVARAFSSRACVALGNLAFAMYILQLPVKAVLLAGVSRFEGRGLS
                     ASWISYLVVLLLVSALAVALIEKPFHRMLVGGRIKSA"
     gene            3379..4329
                     /locus_tag="AC16BC_06895"
     CDS             3379..4329
                     /locus_tag="AC16BC_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HIR3241385.1"
                     /translation="MPDTKIIAVVGATGAQGGGLVRAIMNDKASGFIARAVTRDVSSD
                     KAKALAKLGAQVVAGDVDDQSSLERAFAGAYGAFCVTFFWAHFSPEKELAEATALAKA
                     TKQAGVQHAIWSTLEDTRRWVPLSDNRMPTLMGKYKVPHFDAKGEADQVFAAQGAPTT
                     NLLTSFYWDNFVYFGMGPKKGLDGTLALTFPMGDKKLPGIAAEDIGKCAFALFKNRRE
                     YLGKTVGIAGEHLTGAEMAAAFTKALGRPVRYNAVTPEAYRSFGFPGADDLGNMFQFK
                     RDFNDAFCGARDPKVARSLNPQLQTFEQWLAANKGRIRIE"
     gene            4556..>5246
                     /locus_tag="AC16BC_06900"
     CDS             4556..>5246
                     /locus_tag="AC16BC_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024676.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3241386.1"
                     /translation="MTLARLRDEIDEPCHVRTLRRDLDALTLAGVPVYTEKRNGTTFW
                     RLPDDYRSVPLPVTPTELLALHLAGRLTHPLKGTLFDESMAAAVTKIRTAIAPKTREA
                     FAGLSASVSVGHRPTKDFGPHAKTVEALQTAIQDNRVVRMTYDSHNSGRISLRKANPY
                     HVRFHDNSLYLLAHCHRRKDVRLFAIDRIKALDVTDQRFNPPLFFSPDDYFKHTFGVY
                     QGKKPETVILRF"
BASE COUNT          838 a         1649 c         1772 g          987 t
ORIGIN      
        1 ccgagcggct cgaccatccg ggaactgacc gctcacgatg ccgataccct caagtcgcgt
       61 ttggccgcgg ccgcggatcg atgcgggcag gtcctggtgg atcaattctt ttccggggtc
      121 agatagggga gcatgttcac cgaggagaag acctttctgc ttcgcttcag tctggaggcc
      181 gcgtttccgg aggactacga gggcgaggcg gatcaatacc agtgggtgcg cgagtgggag
      241 ggcggcatga aggccgacgt gatcaaggcc gtgttcagcg ccctgcgcaa tcgtccggga
      301 tggtccgcgc gggtgcgcaa tcggggcatg tcggagcagg acgagatcga aatcgtgctg
      361 tcgcgcgacg cggcggaccc aggctagtcc ttgatgccca cgacgatgaa gtcgggcgga
      421 cgcgtcaccg ggccggagat cgcggtcctc gtcaagtcgc gggaacccac ggtgttacgc
      481 aaggtcgcgc cgtacttgcg gtgggtggcg cggggcgggg ggcgaccggt cgtcgtgtat
      541 ctcgggcggc gggtgcccgc gtccgcgtcg ggcctcctgt tggtgggcgg ggaagacctg
      601 gcccccgctc gctacggcga agcgaatcgc cattgcgaac gcatcaacgc gccgcgcgac
      661 gcgtgggagc tggacctgct gccgcgcgcg ctggatcgcg acgtgcccat cctggccgtg
      721 tgtcggggca tccaggtgct ggcggtggcg ctgggcggga cgttgattca ggacctgccg
      781 atcgagctgg ccgcccgggg cgtgcgatcg cgcgtgatcc accgcggtcc tcgggagacc
      841 gactccgcgc accgcgtcgc gatcgcgccc cgcaccttgt tggcgaagat gattcccgcc
      901 cgcaccgcgc tcgtgaacag ccatcatcac caggccgtgc gcgacgcgcc gtcgaacacc
      961 cgggtgtccg cccggtcgag cgacgccgtg atcgaggcca tcgaacacat tcacaaccgg
     1021 ttcgtgctcg gcgtgcagtg gcacccggag cggtggaacc gcccgtcgtc cgcggccatc
     1081 atgcggggat tcctcgaggc gtgcggcggc gaatgatcgg ggtcgcggcc gcgctgctgg
     1141 cggcgctcgc gtggtggctc gctgcgatgt tctggatgcc gggcgcgtcc gcgcgcggca
     1201 ggtccgagcc gctcaccgcg catgaatccg cgcttgccga cgccctgcgc cgggacgtga
     1261 ccgcgctggg cggcgaaatc ggcgaacgca attttcaaca gtacgacgca ttgctggccg
     1321 ccgccgatta catcgaccac gcgctgactg cgtccggtta cgaggtgcgc cggcagcggt
     1381 acgaggtcga cggccggccg ttcgacaatc tggaggtcga gcgccggggc gcgtcgaagc
     1441 cggatgagat cgttctggtg ggcgcgcact atgactcggt ggtcggcgcg cccggggcca
     1501 acgacaacgg gaccggcgtg gccgcggcgc tggccctggc cgaggcgttc gcggggcgca
     1561 ccacggcccg caccgtgcgc tttgtcgcgt tcgtgaacga agagccgccg tttgcgcaca
     1621 ccccgagtat gggcagcgtg gtgtacgcca cgcgttcgag acagcgcggg gagcgcatca
     1681 cggccatgct gagcctggag acgatgggct acttcgcgga cgcgccgggg agccagcaat
     1741 acccctcggt cctccggtgg gtctatcccg acaccgcgaa cttcatcacg ttcgtgagca
     1801 atctgtcctc gcgcggggag ctcaagcgcg cgatcaagag cttccggcac gtcgcgtcca
     1861 tcccgtccga aggcggcgcg ctcccggcca ctatctcggg cgtggggtgg tcggaccatt
     1921 ggtcgttctg gcaggccggg tttcccgcga tcatggtcac ggacaccgcg ctgtttcggt
     1981 atcccgcgta ccacacgccc aatgacacgc cggagcaggt ggactacgag cgcctggcgc
     2041 gagtggtcgc ggggctggaa cacgtggtcg cggacctggc ggcgtagcgc gtgtcgaaac
     2101 ggccgaccgg cgacgcggca cgcggcgcgg agggttctcc gggctatgtc attcctcaac
     2161 tgaccttcct gcggtttccg gcgatcgtgt ggatcgcgct ctttcacttc ggggcgagcg
     2221 ggttcccgtt ttcggtgagt ccgtttttac tgaaattcgt cggccacggc tacgtggccg
     2281 tgaccttttt ttttgtgctc tcgggtttta ccttggccgt gaggtatgcc ggacgccgga
     2341 tcgagcccac acggttcatg ctggccaggc tggcccgaat ttacccgttg tacctggtcg
     2401 gtctggcggt ctgcgggttg atcttcgcgt atttcggcac gctccaaaga aatcttccga
     2461 cgcttccgct gaacgtcctc ttgatccagg cctgggtgcc gtcattcgcg ctcacgtgga
     2521 acaaaccgtg ctggtttctg tcggttttgt tgttgttcta cctgatattt tgtctgtcgt
     2581 ttgatcgggt cagtcggatg acgccgaaaa ccatggccta ttcgttgacg gtaatctggt
     2641 tggcgagcct cgcggccctg gtgcttctca aggccaccgt gtacaccgga gagccctcgg
     2701 ccatccatga cgtgattttt taccatccga tcatgcacct gaattcattc ttgatcggcg
     2761 tcggcggcgg cctggcgttt cgagccgacg cgttgcagcg tatggccggt gggcggccgg
     2821 ggtgggtgtt ggcgaccggc gcgctcgtcg cgctgctcgg gatcgtggcg gtcgatgatt
     2881 ccgggtacgt gttgcacgac ggattgctgg cgccgctgtt tctggtgttg atcgtcggcg
     2941 tcgcgttgga tacgtcgatg gttgctcgcg cgttttccag tcgagcgtgc gtggcgttgg
     3001 ggaatctcgc ctttgcgatg tatattctcc aactcccggt caaggccgtg ttgctcgcgg
     3061 gagtctcgcg gtttgagggg cgaggcttgt cggcgtcgtg gatatcgtat ctggtcgttc
     3121 tcctgctcgt ctccgcattg gcggtcgccc tgatcgaaaa gccgttccat cgtatgctcg
     3181 tgggcgggcg catcaaatca gcctgaatgg accgtcacga tccgacgcgg tacgcgacgg
     3241 cggatttcgt cgtggacggc ccgatttctt gaccctggag cccccgagag ggtaggctgg
     3301 cgctctattt gtgaacgttg gtcggcgcgt gcctgaccgc attgttgagg acgccgcgac
     3361 tccaaggagg agcatcagat gcccgatacc aagattatcg ccgtggtcgg cgccaccggc
     3421 gcgcaaggcg gcggcctggt ccgcgccatc atgaacgaca aggcgagcgg atttatcgcg
     3481 cgggccgtga cccgggacgt gagttcggac aaggccaagg ccctggccaa gctcggcgcg
     3541 caggtggtgg cgggggatgt ggatgatcaa tccagcctcg agcgcgcgtt cgcgggcgcg
     3601 tacggcgcgt tctgcgtcac gtttttttgg gcgcacttct cgccggaaaa agaactcgcc
     3661 gaagccacgg ccctggccaa ggccaccaag caggccggag tccagcacgc gatctggtcc
     3721 acgctggaag acacgcgccg gtgggtgccg ctgagcgaca accgcatgcc cacgctcatg
     3781 ggcaagtaca aagtgccgca cttcgacgcc aagggcgaag ccgaccaggt cttcgccgcg
     3841 caaggcgctc ccacgaccaa cttgttgacc tcgttctact gggacaactt cgtgtacttc
     3901 ggcatgggcc cgaaaaaagg cctggacggg acactcgcgc tcacgttccc gatgggcgac
     3961 aagaaactgc ccggcatcgc ggccgaagac atcggcaagt gcgcgttcgc tcttttcaag
     4021 aatcgacgcg agtacctcgg caagaccgta ggcatagccg gtgagcacct caccggcgcg
     4081 gaaatggccg cggccttcac caaggccttg ggccggcccg tgcgctataa cgcggtcacg
     4141 cctgaagcct accgcagctt cggcttcccc ggcgccgacg acctgggcaa catgttccag
     4201 tttaaacggg acttcaacga cgccttctgc ggcgcccgcg accccaaagt cgcccggagc
     4261 ctgaatccgc aactgcaaac ctttgagcag tggctggccg cgaacaaggg gcggatacga
     4321 atcgagtagt gaacgcggcg ataactctcc cgtaaggcac gccggattcg gggagccggt
     4381 ggaatggggt cgaagctggt cggcccttat tcgcgacagt tccttgaccc ccaagcccgc
     4441 aacgggctaa gctgggcctt catttccccc aaccacgagc caagggccgc ctgtgccgcg
     4501 caacgatcag gtcatcaggc tgtggaagat tttccgcctg ttagagcgcg gggcggtgac
     4561 ccttgcgcgc ctgcgtgacg agatcgacga accgtgccat gtccgcacgt tgcgccgcga
     4621 cttggacgcg ctgacgctcg cgggcgttcc ggtctatacc gagaagcgca acggcacgac
     4681 attttggcgg cttcctgacg attaccgcag tgtgccgctg ccggtcacgc ccaccgagtt
     4741 gctcgccctg catctggccg gccgactgac gcacccgttg aaggggacgc tcttcgacga
     4801 gtccatggcc gcggccgtga ccaagatccg taccgcgatc gcccccaaga cgcgggaggc
     4861 gttcgcgggc ctgagcgcgt cggtgtcggt cgggcatcgg ccgaccaagg atttcggccc
     4921 gcatgccaag accgtcgagg ccctccagac tgcgattcag gataatcggg tggtccggat
     4981 gacctacgac tcgcacaact ccggtcgcat ctcattgcgc aaggcgaatc cataccacgt
     5041 gaggtttcac gacaacagcc tgtacctgct tgcccattgt caccggcgca aggatgttcg
     5101 cctgttcgcc atcgatcgga tcaaggcgct ggacgtgaca gaccagcggt tcaacccgcc
     5161 attgttcttt tcgccggacg actacttcaa acacacgttc ggggtgtacc aggggaagaa
     5221 accggagacc gtgatcctgc ggttca
//
LOCUS       DBNGTT010000309        13065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2240176, whole genome shotgun sequence.
ACCESSION   DBNGTT010000309 DBNGTT010000000
VERSION     DBNGTT010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13065
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2240176"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="AC16BC_06905"
     CDS             <1..1128
                     /locus_tag="AC16BC_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing transglutaminase
                     family protein"
                     /protein_id="HIR3241387.1"
                     /translation="VEPLDTTVIFAPEHTASLEGPFAAVLMDDDEIFSVPYPPGSRQT
                     YEVWQRTPDARQAVRTSPSRYLRLNGASRIAALAEQTTHGRDTAVEKAGAIERFLRTR
                     YSYTLDVPGGEDRPLDVFLFDRRSGYCEHFASAMAVMLRAVGVPTRLVTGFLVQEWNA
                     VGKYAIVRQGDAHAWVEAYLPNSGWTRFDPTPPVPPADRPWAGSAEHYLDALRTGWNR
                     YVLDFSLRDQHAALENAEVGWSRIRSRITAMLSASTRRLTRVSDATWLAGGLLAAAVF
                     ALGGFLAIRRGRVAVLPSGHAPVAFYHRLLRALERQGVAKPTAATPAEFAQRHAGRPE
                     SAAAIRRVTERYYEVRYGRRRLSPEERRRVEADLATIERDR"
     gene            complement(1157..1552)
                     /locus_tag="AC16BC_06910"
     CDS             complement(1157..1552)
                     /locus_tag="AC16BC_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241388.1"
                     /translation="MTWMVRTACGVVLAAMLTSAATPAIARDDTLPDVLVDGFYGGLI
                     GALLGTAVMVMTEHPRDHLGYITTGAALGAIGGTGYGLAKITRYSMIDIDRQRIAWHV
                     PTVDTTTRTVPGEPTDVRVSAALITVNFR"
     gene            complement(1549..2757)
                     /gene="ribD"
                     /locus_tag="AC16BC_06915"
     CDS             complement(1549..2757)
                     /gene="ribD"
                     /locus_tag="AC16BC_06915"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR3241389.1"
                     /translation="MTHRSAVGRGFRVRARDRPIPASGARVDTATSFADNPAPMSDEV
                     YMRRALTLASRALGRTSPNPAVGAVVVRGGRIVGEGYHRLAGEPHAEVIALRNAGSRA
                     RGATLYVTLEPCCHVDKRTPPCVEAIVSSGIRAVIVATRDPNPKVSGRGIARLRNHGI
                     AARVGVLQDRATRLNEAYAHWITTGKPFVTLKIASTLDGKIATAGGESRWITGPSARD
                     AVHRLRAMVDAVMVGAGTVRTDDPALTARGGRNRRNPHRVVLDERLSVPVTARVFAPH
                     DGSTTFVATTREAPASRRRRLEASGARVLVVGSRGGLVDLKALMRRLGAMGVTSVLLE
                     GGAEVNASALRVGIVNKAVIMLAPKLLGGRDAVGSIGGRSPRRLIDAAVLRDVVVRRA
                     GDDVIVEGYL"
     gene            complement(2708..3505)
                     /locus_tag="AC16BC_06920"
     CDS             complement(2708..3505)
                     /locus_tag="AC16BC_06920"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF014510.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0106408 - diadenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HIR3241390.1"
                     /translation="MSGWDQSVSWSGLFTAAVAASVLVAIGVRFRRSGTYQVLVGFGL
                     VVVGAALGDWSGAWPAGWSAARWWPGVALAALIIFQPEIRKGLAALGRIAGRRSAGPA
                     ARRAVEEVVKAAVALANRRIGALIVIERAQELLDAVEVGTVLDARLSKELLLSVFVPY
                     SPLHDGAVILRDGRIVAAGCFLPLSSRAAGAASYGTRHRAAIGITEETDALAIVVSEE
                     SGTIALVVGGQVARDLDGSALRQRLDAALGADDAPVGRWTWLPRPRT"
     gene            complement(3509..4360)
                     /gene="folP"
                     /locus_tag="AC16BC_06925"
     CDS             complement(3509..4360)
                     /gene="folP"
                     /locus_tag="AC16BC_06925"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3241391.1"
                     /translation="MYDDARTRVIGIVNATPDSFWAGSRTPTARAAVDMAQRFEAAGA
                     DWIDVGGESTGPDSLDVTVDEELRRVVPVVDAVCRAVRIPVSVDTTKAAVAREAVAAG
                     ATMINDVSALRWDPDMAGVAATSNASVILMYAKDDTPRTTRRPVQYDDVVAAVCEFLR
                     ARISHARSAGISEGRIFVDPGLGWFVGADPRYSYEVLAGLDRVRALGHPVVVGPSRKS
                     FLAHDGIHPPRPASERLMPTAAAVAIAAWKGAAIVRVHDVEAMAQVVATVEAIKRPSL
                     LAYPVGG"
     gene            complement(4469..6268)
                     /gene="ftsH"
                     /locus_tag="AC16BC_06930"
     CDS             complement(4469..6268)
                     /gene="ftsH"
                     /locus_tag="AC16BC_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3241392.1"
                     /translation="MNPRMKNLALWIVIGLFMILLFNLFTVPPRTDEADLIFSDFMTK
                     VEKGEVNEVTIKENFLAGVLKDGGKFKTYAAPYPDLINQLRAKNVRIVAKPPDENPWY
                     VTFLVSWGPFILFLGLWIFFMRQMQVGGNKALSFGKSRARLLSEDRKKVTFADVAGVD
                     EAKDEVSEIIEFLKDPPKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTNEGVILVAATNRPDVLDPALLRPGRFDRQVVVARPDLKGRVEILR
                     VHTKKVAMSSDVVLEVVARGTPGFAGADLENLVNEAALLAARKGKKAVEMDDFEAAKD
                     KVLMGVERRSMILSDEEKRTTAYHEAGHALVARLIPGTDPLHKVTIIPRGRALGLTMQ
                     LPTDDRYTYPKEYLYNNIAILMGGRAAEEIVLNFMTTGAGNDIERATELARKMVCEWG
                     MSEKMGPLTFGKKEQEIFLGREISQHRDYSEHTAVEIDQEVRRLVTENYERAKTLIRD
                     NMKALRALAEALLEREVLDAPEIEKIIGAAASA"
     gene            complement(6402..6917)
                     /gene="hpt"
                     /locus_tag="AC16BC_06935"
     CDS             complement(6402..6917)
                     /gene="hpt"
                     /locus_tag="AC16BC_06935"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR3241393.1"
                     /translation="MITQQEIVRRVKELGHRISEDYQGRDVVMIGVLKGAFAFFSDLV
                     RAVQVPVAIDFLVVSSYQSSTRSSGRVKVISDLTLDIKGQDVILVEDIVDSGLTLSYI
                     KKKLLVRHPSSLRVCALLDKPERRTMNVSLEYVGFTVGNKYVVGYGLDYKNQYRNLPY
                     IAVLDQVEDQD"
     gene            complement(6944..8431)
                     /gene="tilS"
                     /locus_tag="AC16BC_06940"
     CDS             complement(6944..8431)
                     /gene="tilS"
                     /locus_tag="AC16BC_06940"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3241394.1"
                     /translation="MTIGVPLRRDARRSIGERLRLSLSRLGPVARGDTLVVGVSGGPD
                     SLTLLHGLRALSREMGFAVHAAHFDHGLRGEQSAAEARVVAEIADRWDVPCTIERAAN
                     GRIEARGRGLQAAARSARYDFLGRVAEAVGAQWIATAHTADDQAETVLMRWLRGAGPA
                     ALAGIPAIRGRVIRPLLGVDRRDIEAYVVEQGLTPLRDPSNEDPRFLRVRVRQDVMPT
                     LRALNPRAVHVIARSAGLLADDAAWLDEQARTAFEHGRVGEGPGWIELHTARFRALPL
                     VIQRRVIRLALSELAVCVDRVSAERIDAAARASVDRTSGCLALGQGAAAEYAAAGLRL
                     TRGPARISPPEGIALPDGECRPPGWDLAVRVTRVAAFAAAGAMGPWQAAFDTGRLPGM
                     LGLRSWRAGDRLDPEGMTGRKRVQDLFVDEKIPRWRRTTAPLVTAGDQVVWVVGFRRD
                     RRYAARPGAPATIVDVVRTPLAEPGADKEATTDARSLYTPNEEGP"
     gene            complement(8428..9375)
                     /locus_tag="AC16BC_06945"
     CDS             complement(8428..9375)
                     /locus_tag="AC16BC_06945"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR3241395.1"
                     /translation="MTSVLRQLPDQPLRAPLVAIGNFDGVHLGHQAIIREVARRAREQ
                     GGTSVVLTFDPHPMSVVHPEAPHSLLTGAEQKIELLTALGVDVVLVLPFTPEFARRSA
                     EQFVREDLGARLGAREVHVGRNFKFGKGREGRVEDLERLGASSGMRVMVRPPVMMGQT
                     MVSSSEIRNRLRDGDVDGAAAFLGRPYAADGMIEPGEGRGRALGYPTANFRPGVQMLP
                     KIGIYAAVVDDLTSGERGLHGVVYIGSQPTFGPHPVQVEVHLFGADTARYDHRLRVAF
                     IRWIRGEERFADSQALIAQIARDVASARAALSQRGEAAR"
     gene            complement(9413..10405)
                     /gene="hemB"
                     /locus_tag="AC16BC_06950"
     CDS             complement(9413..10405)
                     /gene="hemB"
                     /locus_tag="AC16BC_06950"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3241396.1"
                     /translation="MAFPIDRPRRLRRRDVFRRMMRETTLRVDNLIYPLFAVHGTGVS
                     REVASMPGVFQRSVDLLVKDAHEAAALGVPAVLLFGIPATKDAVGSEASSDDGIIARA
                     VRALKSQVPEIAVITDVCLCEYTDHGHCGIVEQGQVVNDATLDRLVSVSLAHARAGAD
                     MVAPSDMMDGRVGAIRRGLDADGFSDVPILSYAAKFASGFYGPFRDAAESTPQFGDRR
                     GYQMDPANGDEALREVALDLEEGADIVMVKPAMPYLDIIRRVKDAYRRPVAAYQVSGE
                     YAMVKAAVQSGWLDEERVAVESLLAIRRAGADLIVTYYAKEAARWMREKGGVLP"
     gene            complement(10466..12004)
                     /gene="cobA"
                     /locus_tag="AC16BC_06955"
     CDS             complement(10466..12004)
                     /gene="cobA"
                     /locus_tag="AC16BC_06955"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3241397.1"
                     /translation="MPNRSSASPAAGTVYLIGAGPGDPGLLTIRGRECLRRADVVVYD
                     YLVHPSLLAEAGPGAEIIDAGKRKGAKTFTQQAINGLLIDRARCGRTVARLKGGDPFV
                     FGRGGEEALALAESGVPFEVVPGVSSVLGAPAYAGIPLTHRGIATTVVLTTGHEDETK
                     GGSGIPWQDVALTSGTLVFVMGMTHLAEIVDRLSGHGRAPDTPVAVIRWGTYPTQRTV
                     VGALATIVRETARADLQPPGLIVVGEVVRLRERLNWFERRPLFGRSVIVTRAEAQAGE
                     LAGLLRDAGADVIEAPAIRLAPPASWREADDAIARLGRYRWVILTSANAVTALFDRVF
                     ARGVDVRALGAARVCAIGTKTAEAMRRYGIVPDRVAEESTGEGVVAALAGETLSGVPI
                     LLPRAKVAREVIVEGLTERGAAVDAVTVYETRSVDRVPDRARDALRRSGTIVTFTSPS
                     TVTGFLDALGAEASLVRDATVACLGPPTAEAATSRGLRVAIQPGQQRMDALVQAIVNH
                     VKTG"
     gene            complement(11988..12929)
                     /gene="hemC"
                     /locus_tag="AC16BC_06960"
     CDS             complement(11988..12929)
                     /gene="hemC"
                     /locus_tag="AC16BC_06960"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR3241398.1"
                     /translation="MATAPSRLVIGTRGSVLARWQAEWVKARLQTAHPGLDVSLTIIK
                     TTGDKILDVPLAKVGGKGLFVKEIEEALLDGSVDLAVHSMKDVPTALPDALHLAAVPR
                     REDPLDALISRDDVPLAKLPPGTTIGTSSLRRQSQLLAHRPDLNIVTLRGNLDTRLRK
                     VADGAMDAIVLAAAGLRRLGWADRITELLTPSVCLPAIGQGALGIECRRADDRVNELL
                     RALDDPDTRACVEAERAFLTRLEGGCQVPIAAHATLAENRLTLDGLIAGVRGERVLRD
                     RITGAREDGPRLGRELADGLLARGGEAILRAVYAESE"
BASE COUNT         2202 a         4863 c         3979 g         2021 t
ORIGIN      
        1 gtcgagccct tggacaccac ggtcattttt gcgcccgaac acacggcatc gttggaaggc
       61 cccttcgcgg cagtcctcat ggacgacgac gagatcttca gcgtccccta tcctcccggg
      121 agccggcaga cctacgaggt gtggcagcgg acaccggatg cgcgccaggc ggtgcgcaca
      181 tcaccctccc gttatcttcg gctgaacggg gcgtcgcgca tcgcggcgct cgccgagcag
      241 accacccacg gccgcgacac cgccgtcgag aaagcgggcg ccatcgaacg gttccttcgc
      301 acccgttatt catatacgct cgacgttcca ggcggcgaag accgcccgct cgacgtattc
      361 ctctttgatc gtcggtccgg atactgcgag cactttgcca gtgccatggc cgtcatgtta
      421 cgcgcggtcg gcgttcccac gcgactcgtc acgggatttc tcgtgcagga atggaacgcg
      481 gtcgggaaat acgccatcgt gcggcagggg gacgcccacg cgtgggtcga ggcgtatctc
      541 cccaactcgg gttggacgcg cttcgatccg acgcctccgg ttccgcccgc cgaccgccca
      601 tgggcgggat cggccgagca ttacctggac gcgttgcgga cgggctggaa tcgctacgtg
      661 ttggatttca gtcttcgcga ccaacacgcg gctcttgaaa acgcggaagt cggttggtcg
      721 cgtatccgct cccggatcac ggccatgttg agcgcctcga cccgccggct gacccgcgtg
      781 tccgacgcca cctggctcgc gggcgggctg ctcgcggccg cggtgtttgc cctcggggga
      841 ttcctcgcaa tccgtcgcgg gcgcgtggcc gttctcccgt cgggccatgc tcccgtcgcc
      901 ttctaccacc gcctcttacg cgcgttggaa cgccaaggcg tcgccaagcc gacggccgcg
      961 acgccggctg agttcgccca acggcacgcg gggcgcccgg agtcggcggc ggccattcgc
     1021 cgcgtcacgg agcgctacta cgaagtccgg tacgggcgac gccgactgtc tccggaagaa
     1081 cgccgccgag tcgaggccga cttggcgacg atcgaacgcg accgctgagc gcgttcccga
     1141 tcgccctcaa cccgcgtcag cgaaaattca ccgtgatgag cgcggcggaa accctcacgt
     1201 ccgtcggttc gccggggaca gttctggtgg tcgtgtcgac ggtcggaacg tgccacgcaa
     1261 tccgttgtcg gtcaatatcg atcatgctgt accgggttat tttggccaga ccgtaacccg
     1321 tcccgccaat cgcccccaag gcggctccgg tcgtaatgta gcccaagtgg tcgcggggat
     1381 gttccgtcat caccatgacc gctgttccca acagggcccc gataagaccg ccgtagaatc
     1441 catcgaccaa cacgtcgggc aaggtatcgt cccgcgcaat cgccggtgtc gccgcactcg
     1501 tcaacatcgc cgccagcacg accccacacg ccgtccgaac catccatgtc ataggtaccc
     1561 ctccacgatc acatcgtctc ccgcacgtcg caccacgaca tcccgcaaga ccgccgcgtc
     1621 gatgagacgc ctcggggatc gcccgccgat cgaccccacg gcgtcacgcc cccccagcag
     1681 cttaggcgcc agcatgatca cggccttgtt gacgattccc acgcgcaacg ccgacgcgtt
     1741 gacttccgct cccccttcga gcaggaccga cgtgacgccc atcgcgccca agcgtcgcat
     1801 cagagccttc agatcgacca gtccgcctcg ggatccgacc acgagcaccc gcgcgccgga
     1861 agcctccagc cgacggcggc gcgaggccgg ggcctcacgc gtcgtcgcga caaacgtcgt
     1921 cgagccgtca tgcggcgcga agaccctcgc ggtcacgggc acggaaagtc gttcgtcgag
     1981 gaccacccga tgggggttcc ggcggttccg tccaccgcgg gccgtcagcg ccgggtcgtc
     2041 ggttcgaacc gtaccggccc cgaccatgac ggcatccacc atggcgcgca gacggtggac
     2101 ggcatcccgc gcgctcggcc cggtgatcca gcgcgactcc ccgccggccg tggcgatctt
     2161 gccgtcgagc gtcgacgcga tcttgagcgt cacgaacggc ttcccagtgg taatccaatg
     2221 cgcgtaggct tcgttgagac gggtcgcccg atcctgaagc acacccaccc gcgccgcgat
     2281 cccgtgattc cgcaaccgcg cgatcccgcg acccgaaacc ttgggattgg gatcacgagt
     2341 cgccacgatc accgcgcgga tgccggacga aacgatcgcc tcaacgcagg gtggggtgcg
     2401 cttgtcgacg tggcagcacg gctcgagcgt gacatacagc gtcgcgcccc gcgcgcgaga
     2461 tccggcgttg cgaagcgcga tcacctcggc gtgcggctcg ccggccagtc ggtgatagcc
     2521 ttccccgacg atgcgtccgc cgcgcacgac cacggcgccc accgcggggt tggggctggt
     2581 gcgccccagc gcccgcgacg caagggtgag ggcccggcgc atatagactt cgtcggacat
     2641 cggcgcagga ttatcggcga acgacgtggc ggtgtcaacg cgagcgccgg aggccgggat
     2701 cggacggtca cgtgcgcgga cgcggaagcc acgtccaacg gccgaccggt gcgtcatccg
     2761 cgcccaatgc cgcgtcgagc cgctgccgca atgcgcttcc gtcgaggtcc cgggccacct
     2821 ggccgccgac gacaagcgcg atcgtcccgc tttcctccga gaccacgatg gccaaagcgt
     2881 cggtctcttc ggtaataccg atggccgcgc ggtgccgggt tccgtacgac gcggcgcccg
     2941 cggcgcgcga cgacaacggg aggaaacatc ccgccgcgac gatccggccg tcacgcagga
     3001 tcacagcgcc atcgtgcagc ggagagtagg ggacgaagac gctgaggagc agctctttgg
     3061 acaaccgggc gtcgagcacc gtgcccactt cgaccgcatc gagcagctct tgcgcccgct
     3121 cgatcacgat caacgcgccg atgcgacggt tcgcgagcgc caccgcggcc ttgaccactt
     3181 cctccacggc tcggcgcgct gcgggaccgg ccgaccgccg accggcaatt cgaccgagcg
     3241 cggccagacc tttacggatt tccggctgaa agatgatgag cgccgccagc gcgacccccg
     3301 gccaccaccg cgcggcggac catccagccg gccacgcgcc cgaccaatcg ccgagcgcgg
     3361 cgccgaccac gacgagccca aaaccgacca ggacctgata cgtgccggaa cgtcggaacc
     3421 gcacgccgat cgcgacgagc accgaggccg cgacggccgc ggtgaacagg cccgaccacg
     3481 atacgctctg atcccatccg gacacgggtt acccccccac cggatacgcg agcaacgacg
     3541 gacgcttgat cgcttccacg gttgccacca cctgcgccat ggcctcaacg tcgtgcactc
     3601 tgacgatcgc cgcccccttc cacgccgcga tcgccacggc ggccgccgtg ggcatcagcc
     3661 gttcggacgc cggccgcggc ggatgaatcc cgtcatgcgc cagaaacgat ttcctggagg
     3721 ggcccacgac cacggggtgt cccaacgccc gcacgcgatc caacccggcc aacacctcgt
     3781 agctataccg gggatcagcg cccacgaacc aacccaggcc gggatccacg aaaatccggc
     3841 cctcgctgat acccgcggag cgggcatggc tgatccgcgc acgcaagaac tcacacaccg
     3901 cggccacgac gtcgtcatac tgcacgggcc gccgcgtggt tcgcggcgtg tcgtccttgg
     3961 cgtacatcaa gatgaccgac gcgttgctcg tggccgccac gccggccatg tccgggtccc
     4021 accgcagcgc ggagacgtca ttgatcatgg tcgcgccggc ggccacggcc tcgcgggcga
     4081 ccgcggcttt cgtcgtgtcg acggaaaccg gaatccgcac ggcccggcac accgcgtcga
     4141 ccaccggtac cacgcggcgt aactcctcgt ccaccgtgac gtccaaggag tccggaccgg
     4201 tggactcgcc gcccacatcg atccagtccg ctccggcggc ttcgaaccgt tgcgccatgt
     4261 ccaccgcggc gcgcgccgtc ggcgtgcggc tcccggccca gaacgagtcg ggggtcgcgt
     4321 tcacgatgcc aataacacgg gtcctcgcgt cgtcatacat ggagtgcctc atcaagtgcg
     4381 gcgtcgcgcg cgagaaccgc gtgcgaccgc caagacgcga ccggggcctc acggccccgg
     4441 cgaaacgatc acggcaaagg gacccggatc aagccgacgc cgcggcgccg atgattttct
     4501 cgatctccgg cgcgtccaac acctcgcgct ccagcaacgc ttcggccaac gcgcggagcg
     4561 ccttcatatt gtcccgaatg agcgtcttcg cccgctcgta gttttccgtg accaggcgcc
     4621 ggacctcctg atcgatttca accgcggtat gttcgctgta atctcgatgc tgagagatct
     4681 cgcggccgag aaagatttcc tgttccttct tgccgaaggt cagcggcccc attttttcgc
     4741 tcatccccca ctcgcacacc atcttgcgcg ccagctcggt ggctcgctcg atatcattcc
     4801 ccgcgccggt ggtcatgaaa ttgaggacga tctcctccgc cgcgcggccg cccatcaaga
     4861 tcgcgatgtt gttgtacagg tactccttcg ggtacgtgta ccggtcgtcg gtcggcaact
     4921 gcatggtaag acccaacgcg cgcccccgcg ggataatcgt caccttgtgc aggggatcgg
     4981 tccccggaat caaccgggcg acgagcgcgt ggcccgcttc gtggtatgcg gtggtccgct
     5041 tctcttcgtc gctgaggatc atgctgcgcc gttccacgcc catcagcacc ttgtccttgg
     5101 cggcctcgaa atcgtccatt tccacggcct tcttgccctt gcgcgcggcc aacaacgcgg
     5161 cttcgttcac cagattctca agatccgcgc cggcaaaccc cggtgtccct cgcgccacca
     5221 cctcgaggac cacgtccgag gacatcgcca ccttcttggt gtgtacgcgg agaatttcca
     5281 cccgtccctt gaggtccgga cgcgcaacca ccacctggcg atcgaaccgc ccgggccgca
     5341 acaacgccgg atccagcaca tcggggcgat tcgtggccgc gaccaggatc acaccttcgt
     5401 tggtgtcaaa cccgtccatt tcaaccaaga gctgattgag ggtttgctcg cgttcgtcgt
     5461 gtccgccgcc aagaccggcc ccgcgcaacc ggccgaccgc gtcgatttcg tcgatgaaga
     5521 tgatgcaggg cgcgtgtttc ttcccctgct cgaacagatc ccgcacccgg gaggcgccga
     5581 ccccgacgaa catttccacg aaatccgacc ctgaaatcga gaagaagggc acgcccgcct
     5641 cgccagcgat ggccttggcc aacaaggtct tgccggttcc gggcggtccc acgatcaaca
     5701 cacccttggg aatccggccg ccgagtttct gaaatttcgg cgggtccttg aggaactcga
     5761 tgatctcgga gacttcatcc ttggcctcgt ccacgccggc cacgtccgcg aacgtgacct
     5821 tcttgcggtc ctcggacagc agccgggccc ggctcttgcc gaacgaaagc gccttattgc
     5881 cgccaacctg catttggcgc atgaagaata tccacagccc gaggaacaag atgaatggcc
     5941 cccaactgac gaggaacgtc acgtaccacg gattttcgtc gggcggcttg gccacgatcc
     6001 ggacgttctt ggcccggagt tggttgatga ggtcggggta cggcgccgcg taggtcttga
     6061 acttcccccc gtccttgagc acgccggcca ggaagttctc cttgatggtg acctcgttga
     6121 cctcgccctt ctccaccttg gtcatgaagt cgctgaagat caagtccgcc tcatcagttc
     6181 gaggtggaac cgtgaacaga ttgaacaaga ggatcataaa cagaccgatt acgatccaca
     6241 gcgccaagtt tttcatacgc ggattcatcc agccgttctc tcctcgtgac tccggccgag
     6301 tcggaatgtc agcgcccgcc ctgcggggcc aagcgcgggg ttgatcttac cacagcgttt
     6361 tctagcttga caaccgagcg cggagcgatg cggagcgccc gtcagtcctg gtcctcgact
     6421 tgatccagga ccgcgatgta cggcaaattc cggtattggt tcttatagtc cagtccgtag
     6481 ccgaccacgt acttgttgcc gaccgtaaac ccgacgtact ccaatgacac gttcatggtg
     6541 cggcgctccg gtttgtccag cagcgcgcac acccgcagcg agctggggtg acggaccagc
     6601 aatttcttct tgatgtaact cagcgtcaac ccggaatcca cgatgtcctc gaccaggatg
     6661 acgtcctgac ccttgatatc cagggtcagg tcggaaatca ccttgacccg gccgctggag
     6721 cgggtggacg actgataact cgacaccacg agaaagtcga tcgccaccgg aacttgtacc
     6781 gcgcggacca ggtcggaaaa aaacgcaaaa gcgcccttga gcacgccgat catgaccacg
     6841 tcgcgcccct ggtaatcctc gctgatccgg tgtcccaact ctttcacgcg ccggacaatc
     6901 tcctgctgcg tgatcatcgg cttgccgaag attctagtca cggtcagggt ccctcctcgt
     6961 tcggagtata tagcgaacgc gcgtcagtcg tcgcctcttt atccgcgccc ggttccgcaa
     7021 gcggggtgcg gaccacgtcc acgatcgtcg ccggagcccc cggacgtgcc gcgtagcgcc
     7081 gatcccggcg aaaccccact acccacacta cctgatcgcc cgcggtcacc aacggcgccg
     7141 tggtccgtcg ccaccgcgga attttctcgt ccacgaacag atcctgcacg cgttttcgcc
     7201 cggtcatgcc ctcgggatcg agacgatcgc cggctcgcca actccgcagg ccaagcatac
     7261 caggaagccg acccgtgtcg aacgcggcct gccacggacc catcgccccc gcggcggcga
     7321 atgccgcgac gcgcgtcacg cgaacggcca ggtcccaccc cggaggccgg cactccccgt
     7381 cgggaagcgc gatgccctcc ggcggcgata tccgggcggg cccgcgcgtc aggcgaagac
     7441 cggccgcggc gtattcggcg gccgcgcctt ggcccagcgc cagacagccc gatgtccggt
     7501 caacggacgc acgggccgcg gcgtcgatgc gttctgccga cacgcggtcc acgcacaccg
     7561 cgagttcgga caacgcgagc cggatgacgc ggcgttggat caccagcggc agcgccctga
     7621 accgcgccgt atgcaattcg atccatcccg gcccctcgcc gacccgcccg tgctcgaacg
     7681 cggtacgcgc ctgttcatcc agccacgcgg cgtcatcggc cagcaaaccg gccgagcggg
     7741 cgatgacgtg caccgcgcgc ggattgagcg cgcggagcgt gggcatgacg tcctgacgca
     7801 cgcgcactcg caggaatctc ggatcctcgt tcgaggggtc ccgcaacggg gtcagcccct
     7861 gttcgaccac gtacgcttcg atgtcacgac gatccacacc caacagcgga cggatcactc
     7921 ggccgcgaat cgcgggaatc ccggccaacg cggctgggcc cgcgccgcgc aaccaccgca
     7981 tcaacacggt ttcggcttga tcgtccgcgg tatgcgcggt ggcgatccat tgcgcgccga
     8041 cggcctcggc gacccggccg agaaagtcgt accgcgcgct tcgcgccgcg gcctgcagac
     8101 cgcggccgcg ggcctcgatc cgcccgtttg ccgcgcgctc gatcgtgcac gggacgtccc
     8161 agcggtcggc gatttcggcc acgaccctcg cttcagccgc ggactgttcg cctcgcaggc
     8221 cgtgatcaaa gtgcgccgcg tgcacggcaa accccatttc tcgcgacaac gcgcgcagcc
     8281 cgtgcagcag cgtcaacgag tcgggccctc cggaaacccc caccaccagg gtgtctccgc
     8341 gtgcgaccgg tccgagacgc gacagggaca gacggagccg ctcgccgatc gatcgtcgcg
     8401 cgtcgcgtcg cagcggaact ccgatcgtca ccgcgccgcc tccccacgtt gcgacagcgc
     8461 ggcccgggcc gacgccacgt cacgcgcgat ctgcgcgatc agcgcttggg aatccgcgaa
     8521 ccgctcctcc ccccggatcc accggatgaa cgccacgcgc aaccgatgat cgtagcgcgc
     8581 cgtgtccgcg ccgaagaggt ggacctccac ctggaccgga tggggaccga aggtcggctg
     8641 cgacccgatg tacaccacgc cgtgcaaccc tcgttcgccg gacgtcaggt cgtccaccac
     8701 cgcggcgtag atcccgatct tgggcagcat ctggacgccc ggcctgaagt tcgcggtggg
     8761 atagcccaat gcgcggcccc ggccttcgcc gggttcgatc atcccgtccg cggcgtaggg
     8821 acgccccaga aacgcggccg cgccgtccac gtcgccgtcg cggagccggt tccggatctc
     8881 ggaggaactg accatggtct ggcccatcat caccggcggc cgcaccatga cgcgcatgcc
     8941 gctcgacgcg cccaagcgct ccaggtcctc cacgcgaccc tcccgcccct tgccgaactt
     9001 gaaattccgc cccacgtgga cttcacgcgc ccccagccgc gcgcccagat cctcccgaac
     9061 gaactgctcc gcgctccgcc tcgcgaactc cggcgtaaag ggaagcacca gcaccacgtc
     9121 cacgcccaac gcggtcaaga gttcgatctt ctgttcggcg cccgtgagca gcgagtgcgg
     9181 cgcttccggg tgcaccacgc tcatcgggtg cggatcgaac gtgagcacca cgctcgtgcc
     9241 gccctgctcg cgcgcgcggc gcgccacctc gcggatgatg gcctgatggc ccaggtgcac
     9301 gccgtcgaag tttccgatcg ccaccagcgg cgcgcgcagc ggctgatccg gcaattggcg
     9361 caggaccgag gtcatcgcgg cgttgtccgt ccccgagtcg cggtaccgag cctcacggca
     9421 acacgccccc cttttcccgc atccaccgcg ccgcctcctt ggcgtagtac gtcacgatca
     9481 ggtccgcgcc cgcgcgacga atcgcgagca gcgactccac ggccacgcgc tcctcgtcca
     9541 accacccgct ctggaccgcg gccttgacca tcgcgtattc gccgctgact tggtaggccg
     9601 ccaccggacg tcggtacgca tccttgaccc gtcgaatgat atccagatac ggcatggcgg
     9661 gtttgaccat cacgatgtcc gcgccctcct ccaggtccaa cgcgacttcg cgcagggcct
     9721 cgtcgccgtt ggccgggtcc atctggtacc cgcggcggtc gccgaactgc ggggtggact
     9781 ccgcggcgtc gcgaaacggc ccgtaaaacc cggacgcgaa cttcgcggcg taggacagga
     9841 tcggcacgtc cgagaacccg tccgcgtcga gcccgcggcg gatcgcgccc acgcggccgt
     9901 ccatcatatc ggacggcgca accatgtccg cgcccgcgcg cgcgtgcgcg agcgacaccg
     9961 acaccagccg atccagcgtc gcgtcgttga ccacctggcc ctgctccacg atgccgcagt
    10021 gtccgtgatc cgtgtactcg cacaggcaca cgtcggtgat cacggcgatc tccggcacct
    10081 gcgacttgag cgcgcgcacc gcgcgggcga tgatgccgtc gtcgctggac gcctcggagc
    10141 cgaccgcgtc cttggtcgcg gggatcccga acaacaacac cgccggcacc cccagcgccg
    10201 cggcctcgtg cgcgtccttg accaacaggt ccaccgaccg ttggaacaca cccggcatcg
    10261 acgccacttc gcgcgacacc ccggttccat gcaccgcgaa gagcggataa atcaggttgt
    10321 ccacgcgcaa cgtggtctcc cgcatcatgc ggcggaacac gtcgcgccgc cgaaggcgcc
    10381 gcggtctgtc gattggaaag gccatgcgtc gttccctcta tcaataagga agccgcgggc
    10441 aggcacgagg cttgccccta caagttcatc cggttttcac gtggttcacg atcgcctgca
    10501 ccaaggcgtc catgcgttgc tggccgggtt ggatcgccac gcgcaaaccg cgcgatgtcg
    10561 cagcctcggc cgtcggaggc cccaggcacg cgaccgtcgc gtcacgcacc aacgaggcct
    10621 cggcacccaa cgcgtccaga aacccggtta ccgtggacgg gctcgtgaac gtgacgatgg
    10681 ttccggagcg ccgcagcgcg tcgcgggcgc gatcaggcac gcggtccacc gatcgcgtct
    10741 cgtacaccgt caccgcgtcc accgcggcgc cgcgctcggt cagtccttcc acaatgactt
    10801 cacgcgccac cttggcgcgg ggcaacaaaa tcggcacgcc ggacaaggtt tcacccgcca
    10861 acgccgcgac cacgccttca cccgtggact cctccgccac tcgatcgggc acgatgccgt
    10921 agcgacgcat cgcctccgcg gtcttggtgc cgatcgcgca cacgcgcgcg gcccccaatg
    10981 ccctcacgtc gaccccgcgc gcgaacacgc gatcgaacaa cgcggtcacc gcgttcgcgc
    11041 tggtcaggat cacccaccgg tatcgcccca gccgcgcgat cgcgtcgtcc gcctcgcgcc
    11101 aggacgccgg cggcgccaag cggatcgccg gcgcttcgat cacgtccgcg ccagcgtcgc
    11161 ggagcaaccc cgccagctcg ccggcctgcg cctcggcccg cgtgacgatc acggagcggc
    11221 cgaacaaggg acgccgctcg aaccagttga gccgttcgcg caatcgcacc acctcgccca
    11281 ccacgatcaa accgggcggc tgcagatcgg cccgcgcggt ctcacgcacg atcgtggcca
    11341 gggccccgac cacggtacgc tgcgtcgggt aggtgcccca gcggatcacc gccaccggcg
    11401 tgtccggtgc gcgtccgtgt cccgacaacc ggtccacgat ctccgccaag tgcgtcatgc
    11461 ccatcacgaa caccaatgtc ccgctggtga gggccacgtc ctgccacgga atcccggatc
    11521 cgcccttggt ctcgtcctcg tgcccggtgg tgagcaccac cgtggtcgcg atcccgcggt
    11581 gcgtgagcgg aatccccgcg tacgcgggcg ccccgagcac cgacgaaacc ccgggcacga
    11641 cctcgaacgg caccccggac tcggccaacg cgagcgcctc ttcgccgccg cggccgaaca
    11701 cgaacggatc gccgcctttg agccgcgcca ccgtgcgccc gcatcgcgcg cggtcgatca
    11761 acagtccgtt gatggcctgc tgcgtaaacg tcttggcgcc cttgcgtttc cccgcgtcga
    11821 tgatttcggc gccgggtccg gcttccgcca ataaggacgg gtgcaccaaa tagtcgtaca
    11881 ccaccacgtc cgcgcgccgc aggcactcgc ggccccggat cgtcagcaaa cccggatcgc
    11941 cggggcccgc accgatcaaa tagaccgtgc cggccgccgg cgaggcgcta ctccgattcg
    12001 gcatagaccg cccgcaggat ggcctcccct ccccgcgcca acaagccatc ggccaactcg
    12061 cgacccaacc ggggcccgtc ttcgcgcgcg cccgtgatcc ggtcgcgcag cacgcgctcg
    12121 ccgcgcacgc ccgcgatcag gccgtccagc gtcagccggt tctccgcgag ggtcgcgtgc
    12181 gcggcaatcg gcacctgaca gccgccctcc aaacgcgtca gaaacgcgcg ctccgcctcc
    12241 acgcacgcac gcgtgtccgg gtcgtccagc gcgcgaagca actcgttgac ccgatcatcc
    12301 gcgcggcggc actcgatccc caaggcccct tgcccgatgg ccggtaggca caccgacgga
    12361 gtcagcaact cggtaatgcg atccgcccac cccagccgcc gcaggcccgc ggccgccaac
    12421 acgatcgcgt ccatcgcgcc gtcggcgact ttacgaagcc gggtgtccag attgccccgc
    12481 agcgtcacga tgttgagatc cggccggtgc gcgagcagtt gcgactgacg ccgcaggctg
    12541 ctggtcccga tcgtcgtgcc cggcggcagc ttggccagcg ggacgtcgtc ccggctgatc
    12601 aacgcgtcca acggatcctc gcgtcgaggc acggcggcca ggtgcagggc gtccggcaac
    12661 gcggtgggca cgtccttcat gctgtgcacg gccaggtcca cggacccgtc cagcagcgct
    12721 tcttcgatct ccttgacgaa caggcccttg ccgccgacct tggccagcgg cacgtccaaa
    12781 atcttgtcgc cggtggtctt gatgatggtc agcgacacgt cgagcccggg gtgcgccgtc
    12841 tgcaggcgcg ccttgaccca ctcggcttgc cagcgcgcca acacgctgcc gcgcgtgccg
    12901 atgacgaggc gagacggcgc cgtggccatc acgtgccgcg cggctccggc cgctcggact
    12961 gcgggtcgac ggcgtgcggc gtttcggtcg tcggcctcga ttccgcggcg ccctcatcca
    13021 ggtgaaacaa gcgtcgcacc aaatccacgt acaccgatcc ctggg
//
LOCUS       DBNGTT010000310         4345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1729466, whole genome shotgun sequence.
ACCESSION   DBNGTT010000310 DBNGTT010000000
VERSION     DBNGTT010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1729466"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC16BC_06965"
     CDS             complement(<1..274)
                     /locus_tag="AC16BC_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241399.1"
                     /translation="MTPWWKGLVIAVVHLALVGSLGAKLLYDRETRPRVWALAASYDP
                     DLPIRGRYVSLQVAVEPRGLDDTDSKAGRQPRPVSLRAEAGRLVADA"
     gene            complement(271..1428)
                     /locus_tag="AC16BC_06970"
     CDS             complement(271..1428)
                     /locus_tag="AC16BC_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HIR3241400.1"
                     /translation="MASRWERYLVRWTNAALIDSVTAERIRAYEAGQDAHQGLRWPTL
                     LAIGLGGLLLGAGVLLFVAAHWDTLSPAERFGLVLALVAVFHLAGALTVERFSALSTT
                     LHAVGTICLGAGIFLAGQIFHLQEHWPGGVLLWAVGAWAAWALLRDWPQAALAALLTP
                     IWLSGEWTEATFGWLGREKILAEGLLLLAITYLTANGPSRESPVRSALVWIGGLALIP
                     LAATVVVSDAMGWRGDLVQPDDSYRTLGWVVALGLPLLVAWALRGYAAWMNGVAALWV
                     VALGTTGFRAAPEAGGFSRAWHELGPYGLCALGAVGLIAWGLNEGRKERINLGVAGFA
                     LTVLTFYSSTVMDKLGRSASLIGLGLLFLLGGWAMEKTRRRLVARLAGDAP"
     gene            complement(1466..2275)
                     /locus_tag="AC16BC_06975"
     CDS             complement(1466..2275)
                     /locus_tag="AC16BC_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA/MotB family protein"
                     /protein_id="HIR3241401.1"
                     /translation="MNTRQLHVLFAVTLAAGLSAGCVSSSKYRALEAKSTADQSELQG
                     QIFDLKSQKQALERDKAGLERDLAALRQEKDRADAEVRSLNERLPQLEQQAAAVSAQK
                     DEEIRRLKGTYDDLVKDLKGEIEKGEIKVTQIRDKLSVNLVEKVLFDSGRAEVKTQGE
                     AVLKKVGAILGEVKDKEIRIEGYTDNVPIGENLRKRFPTNWELSTQRATNVLRFLEEQ
                     AGVDGARLSAIGYGPFRPLADNQTPEGRAQNRRIEIVLVPQDVKEVLKELQ"
     gene            complement(2577..4226)
                     /locus_tag="AC16BC_06980"
     CDS             complement(2577..4226)
                     /locus_tag="AC16BC_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HIR3241402.1"
                     /translation="MLIRALAIASILIVTATVPASTASSNVTAQSRIDAVTAYLNSAR
                     VTRVATIDLPAGDVRVTLEGLTDQLLDDSLRVSGAGDAAAQVFGATVERVPHVETIAK
                     DVRAAEDKLAALKDQDRILDDQLKQAASRRELIDSMRSTYVKERTENLAVRPMDPKEW
                     TGFVDFVAKQYDTVLEQIRKTEVAKRDLAKQIQAAQQALEQIRGRGSLVTKNVAVDLR
                     ADRAGTFELAVSYVVPSASWNPVWDARLDADKQAIALSLYGSVRQFSGEDWTNVALAL
                     STAQPSRGIKVPDLPPQYLEPIRPLPPMAAAPSGMLRGRADTLKAESAAPAFEIMEQA
                     EAAVTQGLLAATFTAPRRESIDSSGTPRRAFLSTFPLKAELTRLAAPKLDEQAVLTAK
                     ATNESGPVLLPGPVNIFLGDELTGKTSLPLTPPGDDIHLAFGPDDRVKVERKIIERKH
                     ETTGLFSKEDIYRYRIRTTVKNLYPTPVTVTILDQVPVSRDETIKVTILDKSTSPTDP
                     EDPTKPGVRRYTYTLQPKAEQVIELGYEVRFPKGQIIGGLE"
BASE COUNT          709 a         1408 c         1426 g          802 t
ORIGIN      
        1 tggcgtcagc caccagccga ccggcttcgg cacgcaggga caccggccgc ggctgccgtc
       61 cggctttcga atccgtgtca tcgagtcctc ggggctccac ggctacttgc aggctgacgt
      121 atcgaccgcg gatgggaagg tcgggatcat aggacgcggc gagggcccag acccggggcc
      181 gcgtctcgcg gtcatagagc aacttggctc cgagcgatcc cacgagagcg aggtgcacca
      241 cggcgatgac gagtcctttc caccacggcg tcacggcgca tcccccgcga gccgcgcgac
      301 caatcgtcgc cgggtctttt ccatcgccca tcctcccagc aggaacagga gccccaggcc
      361 gatcagactg gccgagcggc cgagcttgtc cattacggtc gacgagtaga aggtgagcac
      421 cgtcagcgcg aaccccgcca cgccgaggtt gatccgttcc ttgcgccctt cgttcaaccc
      481 ccaggcgatc agtccgaccg cgcccagcgc gcacagtccg tacggcccca actcgtgcca
      541 tgcccgagaa aatccccccg cctctggagc ggcccggaag cccgtggtgc cgagcgcgac
      601 gacccacaac gcggcgacgc cgttcatcca cgccgcgtag ccgcgcagcg cccacgccac
      661 cagcaggggg agccccagcg cgaccaccca ccccaacgta cgatacgagt cgtccggttg
      721 gacgaggtct ccccgccagc ccatggcatc cgaaaccacc actgtggccg cgaggggaat
      781 cagggccagc ccgccgatcc agaccaacgc gctccggacc ggggactccc tcgacggccc
      841 attggccgtc agataggtga tcgccaggag cagcaggccc tcggcgagaa tcttctcacg
      901 cccgagccat ccgaacgtcg cctcggtcca ttctccgctc aaccagatcg gcgtgagcag
      961 cgcggccagc gcggcttgcg gccaatcgcg caagagtgcc caggccgccc atgccccgac
     1021 cgcccacagc agcacgccgc cgggccagtg ctcttggaga tgaaagatct gcccggccag
     1081 aaagattccg gctcccaggc agatggtgcc cacggcgtgc agcgtggtgg aaagggcgga
     1141 gaatcgttcg acggtcagcg cccccgcgag gtgaaacacg gcaaccaggg cgagcacgag
     1201 tccgaaccgc tcagccggcg agagcgtatc ccagtgcgca gccacaaaaa gcagcacgcc
     1261 cgcgccgagc aataacccgc cgagcccgat ggccaacaag gtcggccacc gcagcccttg
     1321 gtgcgcgtcc tgacctgctt cgtacgcgcg gatgcgctcg gcggtgacgg agtcgatcag
     1381 cgcggcgttc gtccatcgaa ccagataacg ctcccatcgc gaagccacgt catcctcctg
     1441 gaacggggtg gacgagaatc tctgtctatt ggagttcttt taagacctct tttacgtctt
     1501 gcggcacgag cacgatttcg atccggcggt tctgggcgcg tccttccggg gtctggttgt
     1561 cggccagggg acggaaggga ccgtagccga tcgcggacag ccgcgcgccg tccacgccag
     1621 cctgctcttc caggaagcgc agcacattgg tcgcgcgctg cgtggaaagt tcccagttcg
     1681 tgggaaaacg cttgcggaga ttctcgccga tcggcacgtt gtcggtgtag ccctcgatcc
     1741 ggatctcctt gtccttgacc tcgccgagga tcgcgccgac tttcttgagc acggcttcgc
     1801 cctgggtttt gacctccgcg cgcccggaat caaacagcac cttctcgacc aggttgaccg
     1861 acagcttgtc gcgaatctgg gtcaccttga tctcgccctt ctcgatctcg cctttgaggt
     1921 ccttgaccag gtcgtcgtag gttcccttga gccgtcggat ttcctcgtcc ttttgcgcgc
     1981 tgaccgcggc ggcctgttgt tccaactggg gaagccgctc gttcaggctc cgcacctccg
     2041 cgtcggcgcg gtccttctct tggcgcaggg cggccaggtc tcgttccagg cccgccttat
     2101 cgcgctccaa cgcctgtttc tgcgacttca aatcgaagat ttgtccctgc aactcggact
     2161 ggtcagccgt tgatttggct tccagggcgc ggtacttcga cgacgagacg cagccggcgc
     2221 tcaggcccgc ggccagcgtg acggcgaaca gaacgtgcag ttgccgagtg ttcatggttc
     2281 cctccctgat gggcggtaac gggccgcgtc cgtggcccga tgaaggccgg ggtgatgctc
     2341 ccggcgaggg atgccgactc gatccgtccg gacggagtcg ttggtcggcg tagtgtatat
     2401 cgaagcgcac ccgagagtcc acccggatgc cgttgcccgg ttacttcagc cgatcgttga
     2461 tcttctcgat cccggcctgc gcgcacgcct cgtcttgtct ccgccccgcg cggcgccaac
     2521 gccgatggcg gcgatcacct cggcgccgga acttcggtaa tgttctggcg gggcaactac
     2581 tccaatccac cgataatctg tccctttgga aaccgcacct cgtagcccaa ctcgatcacc
     2641 tgttccgcct tcggctgcag cgtgtacgtg tagcggcgca cgccgggttt ggtcgggtct
     2701 tcggggtcgg tggggctggt ggacttgtcg aggatagtga ctttgatggt ctcatcgcgg
     2761 ctgactggga cctggtcgag gatcgtgacg gtcactggtg tggggtagag gtttttcacg
     2821 gtggtgcgga tgcgataccg atagatatct tccttggaga agaggccggt ggtttcgtgt
     2881 ttgcgttcga taattttacg ttcaactttc acgcggtcgt cggggccgaa cgcgagatgg
     2941 atgtcgtcgc cgggcggggt gagcgggagc gaggttttgc ccgtgagttc gtcgcccagg
     3001 aagatgttga ccgggccggg gagcaacacg gggccggact cgtttgtggc cttggccgtg
     3061 agcacggctt gttcgtcgag cttgggcgcg gcgagtcggg tcagctcggc tttgagcggg
     3121 aacgtcgaga ggaaggcgcg tcgcggcgtg ccgctgctgt cgatggattc gcgtcgcggg
     3181 gcggtgaacg tcgcggcaag caggccctga gtgaccgcgg cctcggcttg ctccatgatc
     3241 tcgaaagcgg gcgccgcgct ttcagccttg agcgtatcag cgcgccctct cagcatgccg
     3301 cttggcgccg cggccatggg cggcagggga cggatgggct caaggtattg cgggggtagg
     3361 tcggggactt tgatgccgcg cgagggttgc gcggtggaca aggcgagcgc gacgttcgtc
     3421 cagtcttcgc cggagaattg gcgcacggac ccatagagtg aaagtgcaat cgcttgtttg
     3481 tccgcgtcca ggcgcgcgtc ccaaaccggg ttccaggagg ccgagggcac cacatagctg
     3541 acggccagct cgaacgttcc ggcccggtcc gcgcgcaggt ccaccgcgac gttcttggtg
     3601 accaggctgc cccgcccgcg gatctgctcc agcgcctgtt gcgcggcttg aatttgtttg
     3661 gccaggtcgc gtttcgcgac ttcggtcttg cgaatctgct ccagcaccgt gtcgtattgc
     3721 ttggccacga agtcaacgaa gccggtccac tccttggggt ccatgggccg gaccgcgagg
     3781 ttctcggtgc gttctttgac gtaggtcgag cgcatggagt cgatcagttc gcggcggctc
     3841 gcggcctgct tgagctgatc gtccaatatc cgatcctgat ccttcagcgc cgcgagtttg
     3901 tcctcggccg cgcgcacgtc tttggcaatg gtctccacgt ggggcacgcg ttccaccgtg
     3961 gcgccgaaga cctgggccgc ggcatcgccc gcgcccgaga cgcgcaacga gtcgtcgagc
     4021 agttggtcgg tcagtccctc caatgtcacg cgcacgtcgc cggccgggag gtcgatcgtg
     4081 gccacgcgcg tcacgcgcgc cgagttcaag tacgccgtga ccgcgtcgat gcgagactgc
     4141 gcggtgacgt tgctcgacgc ggtggacgcg ggaacggttg ctgtgacgat gagaatggac
     4201 gcgatggcca gagcacgaat caacatcttg atcctctcct gatgatcgcg gattgggata
     4261 gaccgccgcc acgataacgg atttgggggg agtttgcaaa ggaacccgga acaggggagt
     4321 gaaaacgcgt gagcgtggtt cgacg
//
LOCUS       DBNGTT010000311         6353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2752467, whole genome shotgun sequence.
ACCESSION   DBNGTT010000311 DBNGTT010000000
VERSION     DBNGTT010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2752467"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="AC16BC_06985"
     CDS             complement(<1..214)
                     /locus_tag="AC16BC_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3241403.1"
                     /translation="MRVRMIAAMLTVVGLPAAGLADDRSRPQAVQAVTVRLSEYQYAP
                     ARIEVRKDRPVELRLVNEGKVLHEFVT"
     gene            complement(211..705)
                     /locus_tag="AC16BC_06990"
     CDS             complement(211..705)
                     /locus_tag="AC16BC_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241404.1"
                     /translation="MRDFSRRGYRIGHSAPEIKVIYTGFLVFAAVGYLTLIAIALTRV
                     GPSYQQIVEHYRGSGNEDLLPRPVGQMLEEAHFHAFIQGVTLLVLTHLFVATGGSRRF
                     KRIVVLLAFVGTLCDLASPWLVRFASPGFAALQMVSWIFMTGTAALLIGVPLSDMWWK
                     GAKR"
     gene            complement(702..2204)
                     /locus_tag="AC16BC_06995"
     CDS             complement(702..2204)
                     /locus_tag="AC16BC_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR3241405.1"
                     /translation="MGSILEITLPGIGPERGDELFQAGFAEARRIERLLNAFDPASEL
                     SRLNREACLGPVAVADELVRLIEQARHLSTVSGGAVDATVLPLTRLWRLRDDRHGGTG
                     RPPSSTEISRARASVGFQRVLVDPRRGTVSYNSPNVQLEFGAMGKGYAVDQVVRALVA
                     SGVSSALVDFGSTIYALGRPPGDDAWRVGVRHPRDPHRVLDVVRLVDRALSTSGDDQQ
                     AIVINGVRYGHIIDPRTGQPATGAVAASVIAPTALEADALSTAGFVLGPRDGASLLRR
                     TGRDGIITTETRGRLLNRTDTPGWNAFLARPRQIEGPTRRRILVGMAAAVAAIVLRPA
                     LGHAVVFMSREEALRGLMPEAEGFREEMVSLSDAQRERLASLINGRVSEAEVTFFVGE
                     QSGRVAGYATVLNVIGKEQPITFMVAVSPDGAVRGVQVLTYLESQGSEIRSKRFLEQF
                     AGKTLAAPLKLGRDVHGISGASLSSRSTAYAVKKALALAAVVYGTPGGAW"
     gene            complement(2244..3449)
                     /locus_tag="AC16BC_07000"
     CDS             complement(2244..3449)
                     /locus_tag="AC16BC_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241406.1"
                     /translation="MRWTVAVLAGCAWLVGQEAVNARASDSVTERLDRIESRLDQLGG
                     GGDESHGGTGRLSWHGYGELHFNSPEGSGVPDDADPAIMDLHRMVWGLSYQFNDRISL
                     HTEVDFEHAAQEMELEFAYVDFAITPAFNVRAGSMLMPVGPLNEFHEPTLFYSVERPY
                     VQRTIIPTSWQEGGAGVFGAVNGLKYRVYLVSGLNAEGFTASDGIRGGRGGVAEQPSE
                     DLAVVGRLEYVGLPGVELGLSAYQGGANTTKNPALSDAGVGIWEADARLRMAGLDLRG
                     VLASVTVDGADQITAFTIADPTVDDQLVGEEMMGWYLEAAYDVLKLVNPEGDQALQVF
                     VRYEDFNTQKSVPTGFTAVPANDRQVMTAGLAYFPIPDVAVKADLESWEDGSDNDGSR
                     FNVGVAYQF"
     gene            complement(3883..4356)
                     /gene="bfr"
                     /locus_tag="AC16BC_07005"
     CDS             complement(3883..4356)
                     /gene="bfr"
                     /locus_tag="AC16BC_07005"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR3241407.1"
                     /translation="MKAKEGVVDLLNRVLTNELTAINQYFVHAEMCHHWGFERLYEKI
                     RKHSFDEMKDAHELIEHILYLDGIPNLQRLGQVQIGETVLEQLNLDLRLEQDAVVALT
                     EGIVHCAKVGDFNTRARLERMVHGEEGHIDWIETQLDAIKRVGIENYLSQQLTKE"
     gene            complement(4444..4689)
                     /locus_tag="AC16BC_07010"
     CDS             complement(4444..4689)
                     /locus_tag="AC16BC_07010"
                     /inference="COORDINATES: protein motif:HMM:NF016230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3241408.1"
                     /translation="MYICLCKGITDSRVRALGRNGVVGPEALAAALGIDRDDCCGRCL
                     AGIENLVAIAEAERLIADHPEPAGTSISSSARCSRSH"
     gene            complement(4777..6225)
                     /locus_tag="AC16BC_07015"
     CDS             complement(4777..6225)
                     /locus_tag="AC16BC_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3241409.1"
                     /translation="MALSVGAARAEEAPSVLSLTLQECIERALGANLDLQIERVTPAI
                     SEWNIVSAEGAFDPALTGSINYQNSLEPLSPERKAALNRDSLKDQQLQTNLSLIGTLQ
                     TGTRYELSAFDTLSGGTLAPDGIHTGTTGLTLTQPLLKNFGRSNTVAVRVARSDRQIA
                     IEQTAGRVMDIVSDVSRSYYELVFAIEDHAAKLEDLQRAKALLADNRTRVEVGVMSPL
                     DVTQAEAGAAEREEAVIVAERAIIERENALKRLIASDVTTLRGVSLRPSESLPEDAVA
                     TDAAESTRIALTGRPEVKQARHEVERQSLLRAFNRNQALPEVNLETSYGLNARGDTPR
                     EWAHDLKDRNNPVWSVGLTATMPLGNRQADAAYRIARLEEERAGLTLRKLEQDIIVQV
                     DNAVGRVRTNGKRVEATRVARRLAEESLKAEEARLRAGASTSFLVLQAQSQLAAARSA
                     EIRAKADYGTSLVDLARVEGTTLAKHHITLDE"
BASE COUNT         1138 a         2248 c         1794 g         1173 t
ORIGIN      
        1 ccgtgacgaa ctcgtgcagc actttcccct cgttcacgag ccgcagttca accggccgat
       61 ccttgcgaac ctcgatgcgc gcgggcgcgt actgatactc gctcaagcgg accgtgaccg
      121 cctgcaccgc ctgggggcgc gaccggtcgt ccgccagccc cgccgccgga agccccacca
      181 ccgtcagcat cgcggcaatc attctcaccc tcatcgtttg gctcctttcc accacatgtc
      241 agacaaggga acgccgatca acagcgccgc cgtccccgtc atgaatatcc aactcaccat
      301 ttgcagcgcc gcgaaccccg gcgacgcgaa acgaaccaac cacggcgacg ccaggtcgca
      361 gagcgtgccc acgaacgcca ggaggaccac gatccgtttg aaccggcggc tgcctccggt
      421 ggccacgaac aagtgcgtca acaccagcaa cgtgactccc tggatgaacg cgtgaaaatg
      481 cgcctcctcc agcatctgcc cgaccgggcg cggcaggagg tcttcgttcc cggatccccg
      541 ataatgctcg acgatctgtt gataggacgg tcccactctg gtcagcgcaa tggcgatcaa
      601 ggtgagatac ccgaccgcgg cgaagaccag aaatccggta taaatgacct tgatctcggg
      661 ggcactgtgt ccgatccgat agccgcgacg ggaaaaatct ctcaccatgc gcctcccggg
      721 gtgccgtaga ccaccgcggc cagagccagg gcttttttca cggcataggc ggtggatcgc
      781 gacgacaggc tcgccccgct gatgccgtgc acgtcccggc ccagtttgag cggcgccgcc
      841 aatgttttgc ccgcaaattg ttcaagaaac cgtttcgatc ggatctccga tccctgtgat
      901 tctagataag tcaggacctg gaccccgcgg accgcgccat cgggcgagac cgcgaccata
      961 aaggtgatgg gttgctcctt gccgatcacg ttcagcacgg ttgcgtaacc cgctacgcgt
     1021 cctgactgct cccccacaaa gaacgtgacc tcggcctcgg aaacgcggcc gttgatcaag
     1081 gacgcgagcc gctcacgctg cgcgtcgctc agcgacacca tttcttccct gaatccttcg
     1141 gcctcgggca tcagaccgcg aagcgcctcc tcccggctca tgaagactac ggcatggccg
     1201 agcgcggggc ggagcacgat ggccgccacg gccgccgcca tccccacgag aatccggcgc
     1261 ctggtggggc cctcgatctg tcgcggccgt gccaggaacg cgttccagcc cggcgtgtcg
     1321 gtgcggttca gcagtcttcc cctggtttca gtcgtgatga tcccatcccg ccctgtcctg
     1381 cgtaacagcg atgcaccatc ccgcggtccc aagacaaatc ctgcggtgga cagggcgtcg
     1441 gcttccagcg cggtgggcgc tatcaccgac gcggccacgg cgcccgttgc cggctgtccg
     1501 gtgcgcgggt cgatgatgtg cccgtagcgc acgccgttga tcacgatcgc ctgctggtcg
     1561 tctcccgacg tggacaacgc gcgatcgacc agcctgacca cgtcgagcac gcggtgcgga
     1621 tctctcgggt gccggacccc gacgcgccac gcgtcgtcac ccggtggtct cccgagcgcg
     1681 tagatggtgc tgccgaaatc caccagagcc gacgatacgc cggacgcgac aagggcccgc
     1741 accacctgat ccacggcgta ccccttgccc atggcgccga actccagttg cacgttcgga
     1801 gagttgtacg atacggtgcc gcgcctggga tcgaccagca cgcgctgaaa tcccacgctc
     1861 gcccgcgccc ggctgatctc agtggaagac ggcggtcgcc ccgtgccgcc atgtcggtca
     1921 tcgcgcaatc tccacaaccg cgtgagcggc aagaccgtgg catccacggc cccgccgctc
     1981 acggttgaca aatgccgagc ctgctcgatc aaccggacca gttcgtcggc caccgccacc
     2041 ggcccgaggc acgcctcgcg attgaggcgc gagagctcgc tcgcgggatc aaacgcgttc
     2101 aacagccgtt cgatccgacg ggcctcggca aacccggctt gaaacaattc gtcgccgcgc
     2161 tcgggaccaa tgccgggaag cgtgatttcc aaaatgctcc ccatgcacgg gcgacttgtc
     2221 ttactgcgcg gcgctcccat cacctagaac tgatacgcca cgcccacgtt gaaccggctc
     2281 ccgtcgttgt cggatccgtc ttcccaactc tccaggtccg ccttgaccgc cacgtccgga
     2341 atcgggaagt acgccagccc agcggtcatc acttgccggt cattcgccgg gacggcggtg
     2401 aagccggtcg gaaccgattt ctgggtattg aaatcctcgt aacgcacgaa gacctgaagc
     2461 gcctggtctc cttcgggatt caccaatttg aggacgtcgt aagcggcctc gagataccac
     2521 cccatcatct cttcgccgac aagctgatca tcgacggtcg gatccgctat tgtaaacgcc
     2581 gtgatctgat ccgcgccgtc caccgtgacc gacgccagca ccccgcgcag atcaaggccc
     2641 gccatgcgca gtcgcgcgtc agcctcccag atccccaccc cggcatccga caacgccggg
     2701 ttcttcgtgg tattggcgcc tccctggtac gccgacagcc cgagttccac gccgggcaat
     2761 cccacgtatt ccaaccgtcc gaccacggcc agatcctccg acggctgctc cgccacgccg
     2821 ccgcgtccgc ctcgaatgcc gtcgctcgcg gtgaagccct cggcgttgag ccccgagacc
     2881 aaatagacgc ggtacttcag accgttgacc gcgccgaaca cgccggcccc gccttcctgc
     2941 cacgaggtcg ggatgatggt gcgctgcacg tacggccgct cgacgctgta aaacagcgtg
     3001 ggttcatgaa attcgttcaa ggggcccacc ggcatgagca tgctgccggc ccgcacgttg
     3061 aacgccggcg tgatcgcgaa atccacatag gcgaattcga gctccatctc ctgcgccgcg
     3121 tgctcgaagt cgacctcggt gtgcaaactg atccggtcgt tgaactgata gctgagcccc
     3181 cacaccatcc ggtgcagatc catgatcgcg ggatccgcgt catcgggaac gcccgacccc
     3241 tcggggctgt tgaaatgcag ctccccgtac ccgtgccaac tcaaccggcc cgttcctccg
     3301 tgcgattcgt ctccaccgcc cccgagttga tcaagccgcg attcgatccg gtccagccgc
     3361 tccgtcaccg agtccgaggc gcgcgcgttg accgcctcct ggcccaccaa ccatgcgcat
     3421 cccgccagca ccgccaccgt ccatctcatc actgttcctc cgtggatgtt ggtcatgtcg
     3481 accgttgggc gctgggtgct ccggatgctt atgaccgctg gcctccgtgg tgtaatcgcc
     3541 ctcatcgctt agggcgcttc ctcattacgc ggacgggtcg ccgatccccc gatccgattg
     3601 gaagggatca gcagcgttcg cttgcatcgg cgacacttca gttcaaggga cctgccacgc
     3661 caccgcgcca acaaccctcc acacaaacag cgcacgtcgc ccgtacctcc gcactcttcc
     3721 gatcctcggg cggtcatggc gcctcactat tgatattgga attagatttc attttcataa
     3781 ccacgaggcg cgatcatagt cggaatgaca acggcttgtc aagggaacga tcagtcgacg
     3841 ggacggcgag ccaatgacat ccggcgggat cgacgcgtga acctactcct tcgtcaattg
     3901 ctggctgagg tagttttcaa tgccgactcg cttgatcgca tccagttgcg tttcgatcca
     3961 atcgatgtgc ccttcctccc cgtgcaccat ccgttccagc ctggcacggg tgttaaagtc
     4021 tcccaccttc gcgcaatgaa cgatcccctc ggtcagcgcg acgaccgcgt cctgctcgag
     4081 gcgaagatcc aggttgagtt gttcgagcac ggtctccccg atctgcacct ggcccaaccg
     4141 ctgaagattc gggatgccgt cgagatataa gatgtgctca atgagttcgt gggcgtcctt
     4201 catctcgtca aagctgtgct tgcggatctt ttcatacaac cgctcgaatc cccagtgatg
     4261 acacatctcg gcgtgcacga aatattgatt gatcgcggtc agttcattgg tcaacacgcg
     4321 attcagcagg tcgaccactc cctctttagc tttcatgccc ttcctccacg gcgacggacg
     4381 atgcgcccca acccacgttc cccaaacgag cgggtggcac cgtactcctc cctgccgttg
     4441 atgtcaatga gaccgggagc accgggccga cgatgaaatc gacgtgccgg caggctccgg
     4501 atgatccgcg atgagtcgct cggcttcggc gatggcgacg aggttttcga tgcccgccag
     4561 gcaacgcccg caacaatcgt cccgatcgat ccccaatgcc gccgccaacg cctccgggcc
     4621 aacaaccccg ttccgcccca gcgcccggac ccgggagtcc gtaatgccct tgcacaaaca
     4681 gatatacatg cgtcgatgcc ctctagctat gatattgaat ttctttttca ttaccatagc
     4741 ctaactcgcc tccccgtgtc aagcggcacg gcggacctat tcgtccaacg tgatgtgatg
     4801 tttggccaac gtggtgccct cgacgcgggc caagtccacc agcgacgtgc cgtaatcggc
     4861 ctttgcgcga atctcggccg atcgcgccgc ggccaactgc gactgcgcct ggagcaccaa
     4921 aaaactggtg gacgcgccgg cgcgcagccg ggcttcttcg gccttgagcg actcttcggc
     4981 cagccgcctc gccacccgcg tggcctcgac ccgcttgccg ttggtccgga cccggccgac
     5041 cgcgttgtcg acctgcacaa tgatgtcctg ttccaacttg cgaagggtca accccgcccg
     5101 ctcctcttcc aggcgcgcga tccgatacgc cgcgtcggcc tgccggttac ccagcggcat
     5161 cgtggcggtc aagcccacgc tccacaccgg gttgttgcga tccttcaggt cgtgcgccca
     5221 ctcacggggg gtgtcgccgc gcgcattgag cccatagctc gtttcgagat tgacctccgg
     5281 caacgcttga ttccgattga atgcgcgcaa gaggctttga cgttccactt cgtgtcgggc
     5341 ttgtttgacc tccggacggc ccgtcagcgc gatacgcgta ctctccgcgg cgtcggtcgc
     5401 gaccgcgtcc tccgggagcg actcggacgg ccgcagcgaa actcctctga gcgtggtcac
     5461 gtcggacgcg atcagccgct tgagcgcgtt ctcgcgctcg atgatcgccc gctcggcgac
     5521 gatcacggcc tcctcccgct cggccgcgcc cgcctcagcc tgcgtcacgt ccaacggcga
     5581 catcacgccg acttcgaccc gcgtccggtt gtccgccagc agcgccttgg cccgttggag
     5641 gtcttcgagc ttggccgcgt ggtcctcgat cgcaaatacc agctcgtagt aggaccgact
     5701 cacgtcgctg acgatgtcca tgaccctccc cgcagtttgc tcgattgcga tctgccgatc
     5761 gctgcgggcg accctgaccg cgaccgtgtt cgagcggccg aagttcttga gcaacggctg
     5821 ggtcagcgtg agaccggtgg ttccggtatg gatgccgtcg ggcgcaagcg tgccccccga
     5881 taacgtatcg aaggcggaca actcatagcg cgtcccggtc tgcagggtcc cgatcaacga
     5941 caggtttgtt tgcaactgtt ggtccttgag tgaatcgcga ttgagcgctg ctttgcgctc
     6001 gggcgacagc ggctccaacg agttctgata gttgatcgac cccgtcaacg ccgggtcgaa
     6061 cgccccctcg gcgctcacaa tgttccactc gctgatcgcc ggcgtcacgc gttcgatctg
     6121 cagatccaga ttcgcgccca gcgcgcgctc gatgcactcc tgaagggtca gcgacagcac
     6181 gctcggcgcc tcctcggctc gcgcagctcc aacgctaagt gccatcgcga ctactacgat
     6241 gaccgaacga tgaaacagac acgccaagcc gacccccctt agaagtctac ccgcatcatc
     6301 tgatgcggga gccggttcag gagggcgtca gatgccccct tcggggactt ccg
//
LOCUS       DBNGTT010000312         4816 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1346655, whole genome shotgun sequence.
ACCESSION   DBNGTT010000312 DBNGTT010000000
VERSION     DBNGTT010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1346655"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..176
                     /locus_tag="AC16BC_07020"
     tRNA            101..176
                     /locus_tag="AC16BC_07020"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Lys,seq:ttt)
     gene            273..350
                     /locus_tag="AC16BC_07025"
     tRNA            273..350
                     /locus_tag="AC16BC_07025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:309..311,aa:Glu,seq:ttc)
     gene            complement(434..1135)
                     /locus_tag="AC16BC_07030"
     CDS             complement(434..1135)
                     /locus_tag="AC16BC_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3241410.1"
                     /translation="MEEPPIGILLAEDNPADVEIIRRSLHKGRLANPLVVARDGQIAL
                     DLLRDPASRFGVVILDIHLPKVGGLEVLKEAKSIDPELVVIMLTGQASFQTAVQALRH
                     EGAFDYVQKSKDDLPDLVEAVRLALKKHSVRREALWTVGSKTDGTRTVDMHTIKERFD
                     LSDREVDVIKCLCRGDTNKEIAERLFISDLTVKGHLKHIYQKMGVHTRSAVVSTVLAN
                     APSTTASSRRSNSPV"
     gene            1291..2424
                     /locus_tag="AC16BC_07035"
     CDS             1291..2424
                     /locus_tag="AC16BC_07035"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3241411.1"
                     /translation="MTSGDGAVIRPNHAEALFRSLLEAAPDAIILADAAGRIVLVNAQ
                     AESLFGYRREELVGQQVEMLIPERVRARHIPHRMQYLGSPKTRPMGAGLDLAGRRQDG
                     SEFPVEISLSPMATSDGMLVISIIRDVTDQQRVAQALRLQTKRLEEKVREMDDFIHVV
                     SHDLKEPLRGIEAFAGFLLEDYGDRLDEQGKRYLGFLKQSAVRMKDLIHDLLTLASLS
                     RKEPARQSVELREIVARMERDLAYAIGSRHAEIRVAGSLPPVTGDPTRLGELFKNLVS
                     NAIKFNVSERPVVTISSSEEHDMVRVAVADNGIGIDPRYHERIFDLFERLHPQEEFEG
                     TGAGLAICRKIVEAYGGRIWVESAPGRGSTFVLTLPKAEKGVP"
     gene            2421..2876
                     /locus_tag="AC16BC_07040"
     CDS             2421..2876
                     /locus_tag="AC16BC_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3241412.1"
                     /translation="MTSDIRPVSILLVEDNLQDVEITRRAFAKGRVRNDLTVVRDGEE
                     ALDYLFRRGKYRDPASSPRPGMILLDLNLPKLGGLEVLQQIKNDESLKTIPVIVLTVS
                     QREQDIVRSYNLGVNTYIQKPVEFENFMHVVNTVHDYWILIATLPPSST"
     gene            3012..4370
                     /locus_tag="AC16BC_07045"
     CDS             3012..4370
                     /locus_tag="AC16BC_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR3241413.1"
                     /translation="MRVTFLGATGTVTGAKFLVTAARRRILVDCGLFQGFKQLRLRNW
                     TPPPINPASIHAVVLTHAHLDHSGYLPVLIKQGFAGPVFCSAGTRDLCRVLLPDSGFL
                     QEEEAQYAARHGYSKHEIPLPLYTRLEAERALTHLVPIEVGKAHDLGSGVTARLDPSG
                     HLLGASLVTLTMGGTTVTFTGDLGRPHDAIMQPPVALAAADYLVLESTYGDRRHDPTD
                     PGDVLEAVINRTAARGGVLIIPAFAVGRAQTMLYYVERLKAARRIPDIPVFLNSPMAV
                     DATEVYWAYHREHRLSLDQCRAIGRAARFVTDVQESKRLNTLDGPMIIISASGMATGG
                     RVIHHLKAFAPDPKNTILFAGYQVPGTRGASLVAGARTVKIHGEYVPVNAEVVAMNNV
                     SAHADADEIMAWLRQFRSPPRRTFLTHGEPSAADALRHRIEEELGWSCRVPEYREVAR
                     LT"
     gene            4367..>4816
                     /locus_tag="AC16BC_07050"
     CDS             4367..>4816
                     /locus_tag="AC16BC_07050"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482146.1"
                     /note="catalyzes the formation of thymine and
                     2-deoxy-alpha-D-ribose 1-phosphate from thymidine; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR3241414.1"
                     /translation="MTPIDPPPAAGEHRLRIKRLGIDTYQEPVIYMRQDCDVCRSEGF
                     EAQSRVEIRRDGRPIVATLNVVTSDLLARNEAGLSEAAWRLLGASPGDSITVRHPPPL
                     ESLGFVRAKVHGKRLDAGAITAIVRDIAAGRYADVELAAFLTAGAGSG"
BASE COUNT          853 a         1486 c         1593 g          884 t
ORIGIN      
        1 acgcgcgtct gatacgatcc gccgccttgc ttcgccatgt gcgatgaagc ctccggcgca
       61 ttgatcggcg cctgacggcg caagcacggt tggaaggtgt gagccgctag ctcagttggt
      121 agagcaccgc ccttttaagg cgggtgtcct gggttcgagg cccaggcggc tcaccaattt
      181 tttcgtaagc gaaaacggag cagaggccaa aaacatctcc gagcctcggc gctgcaccgc
      241 gcgcctccgt tcggctacgc aagcgaggtt gtgtccccat cgtctagccc ggcctaggac
      301 accgcccttt cacggcgata acacgggttc aaatcccgtt ggggacacca cgctcaaact
      361 cacccaccgc gattccgcgc aagacggacc cccccccccc cgttcgaggg atggtgtttt
      421 tccgccggta gtttcaaact ggtgaattag accgacggga tgacgcggtc gttgacgggg
      481 cgttggcgag aaccgtcgag accaccgccg aacgcgtatg aacgcccatt ttctggtaga
      541 tatgtttgag atggcctttg acggtgagat cgctgatgaa caatcgctcc gcgatctcct
      601 tgttcgtgtc gccccggcac aggcacttga tcacgtcgac ttcacggtcc gacaggtcga
      661 accgctcctt gatcgtgtgc atgtcgacgg tgcgggtgcc gtctgtcttg gatccgacgg
      721 tccagagcgc ctcgcgtcga acggagtgtt ttttgagcgc caagcggacc gcctcgacca
      781 ggtccgggag atcgtccttg gatttttgca cgtagtcgaa ggcgccctcg tgacggaggg
      841 cttgcaccgc ggtctgaaag gacgcttgtc cggtcagcat gatgaccacc agttcgggat
      901 cgatcgactt cgcttccttc agcacctcaa ggcccccgac ttttgggagg tgaatgtcga
      961 ggatcaccac gccgaaccgg gaggccgggt ctcgcaggag gtccaacgcg atctggccgt
     1021 cgcgagccac cacgagcggg ttggcgagac gtcctttgtg gagcgaccgc cgaataatct
     1081 cgacatcggc ggggttgtct tcagcgagca agatgccgat cggtggttct tccatcgtca
     1141 ggtactcgcg gcggagtcga agaccattcg tggctcgatt ataccggttg gattttcgcg
     1201 tggtcaacga tgatcagcgc cggaggcccg tcgtgcccgt gaaccggcgt cgggcgccgg
     1261 caacgtggtc gtcgtggccg ggggtgccgc atgacttccg gtgacggggc ggtgatccgg
     1321 ccgaaccacg ccgaggcgct gtttcggagt ctcctcgaag ccgcgccgga cgcgatcatc
     1381 ttggccgatg cggccgggcg gatcgtcctg gtcaacgccc aagccgaatc cctgttcggg
     1441 tatcgtcgcg aggaactggt gggccagcag gtggaaatgt tgattcccga gcgcgtgcgc
     1501 gctcgccata tcccgcatcg gatgcagtac ctgggcagtc ccaaaacccg tccgatgggc
     1561 gcggggctcg atctcgccgg ccgtcgtcag gacggcagcg agtttccggt ggagatcagc
     1621 ctgagcccca tggcgacctc cgatggcatg ctggtcatca gtattattcg agacgtgacc
     1681 gaccagcagc gggtggcgca agcgctgcgg ctccagacga agcgcctcga agagaaagta
     1741 cgcgaaatgg atgacttcat ccacgtggtt tcgcacgatc tcaaagagcc gctgcgcggg
     1801 atcgaagcgt tcgccggatt cctgttggag gattacggcg atcggttgga tgaacagggc
     1861 aagcgatacc tgggctttct caagcagtcg gcggtccgga tgaaggacct gattcacgac
     1921 ctgttgacgc tcgcgtcgct ctcgcgcaag gagccggcgc ggcaatccgt ggaattgcgc
     1981 gagatcgtgg ccagaatgga gcgcgatctg gcctatgcga tcgggagccg acacgcggag
     2041 attcgcgtgg ccgggtcgct gccgcccgtg accggcgatc ccacgcggct cggcgaattg
     2101 ttcaagaacc tggtgtcgaa tgccatcaag ttcaacgtca gcgagcgtcc cgtcgtgacg
     2161 atttcctcgt cggaggagca cgacatggtc cgcgtcgcgg tggcggacaa cggcatcggg
     2221 attgatccgc gctaccatga acgcatcttc gatctcttcg agcggttgca cccgcaagag
     2281 gaattcgagg ggaccggggc cggattggcg atctgccgga agatcgtgga ggcgtacggc
     2341 gggcggatct gggtggaatc cgcgcccggc cgcggcagca cgttcgtgtt gaccctgccc
     2401 aaagcggaga aaggcgtgcc atgaccagcg acatacgtcc ggtatccatc ctgctcgtgg
     2461 aggacaatct ccaggatgtt gaaattacac gacgcgcctt tgccaagggc cgcgttcgca
     2521 acgacctgac ggtggtgcgc gacggcgagg aagcgctcga ttatctcttc cgccgcggca
     2581 agtatcggga tccggcctcg tctccgcgcc ccgggatgat cttgctcgac ctcaatctgc
     2641 cgaagctggg cgggctggag gtgctgcaac agatcaagaa cgacgagtcg ctcaagacga
     2701 tacccgtgat cgtcctgacc gtatcgcagc gcgaacaaga catcgtgcgc agctacaatc
     2761 tgggcgtgaa cacctatatc caaaagccgg tcgagtttga aaatttcatg cacgtcgtca
     2821 acaccgtgca tgactactgg atcttgattg ccacccttcc cccgtcctcg acctgatcga
     2881 gtcgcgggcg tcggccctgt tcgatgagat ccgtcgatac cctccggccg atcgaacggc
     2941 cttgtcgggc cgaggccatg gcatccggac gtcaggtgtg atagaggaga ggggtgacgg
     3001 cgtctgatcc catgcgcgtg acgttcctcg gcgccaccgg aacggtcacg ggggccaagt
     3061 ttctggtgac cgcggcccgg cgccggatac tggtggactg cgggttgttt caagggttca
     3121 agcaactccg actgagaaat tggacgccgc cgccgatcaa tcccgcgtcg atccacgcgg
     3181 tcgtgttgac gcacgcccat ctggaccaca gcgggtatct cccggttctg atcaagcaag
     3241 ggtttgccgg cccggtgttc tgtagcgccg gcacgcggga tctgtgccgg gtcttgctgc
     3301 cggacagcgg ctttctgcag gaagaagaag cgcagtacgc ggcccgccac gggtattcca
     3361 aacacgaaat tccgttgccc ctgtacacgc gcctggaggc ggaacgcgcg ctcacccatc
     3421 tggtgccgat cgaggtcggg aaggcgcatg atctggggag cggggtgacg gcccggctcg
     3481 atccgtccgg gcatttgttg ggcgcgtcgc tcgtgacgct gacgatgggg ggtaccacgg
     3541 tcacgttcac gggcgatctt ggccgtccgc acgatgcgat catgcaaccg ccggtcgcgc
     3601 tcgcggccgc ggactatctc gtgctcgagt ccacctacgg cgatcgccgc cacgatccga
     3661 ccgacccggg ggacgtgctg gaggcggtca tcaaccggac cgccgcgcgc ggcggggtgc
     3721 tgatcattcc cgcgttcgcg gtggggcgcg cccaaaccat gctgtattac gtcgaacggt
     3781 tgaaggcggc caggcggatt ccggacatcc cggtgttctt gaacagtccg atggcggtcg
     3841 acgcgaccga agtgtattgg gcctatcatc gcgagcatcg tctctccctg gaccaatgcc
     3901 gggccatcgg gcgggcggcc cgtttcgtga ccgacgtgca ggaatccaag cggctcaaca
     3961 cgctcgacgg ccccatgatc atcatttccg cgagcggcat ggcgacgggc gggcgcgtga
     4021 tccaccatct caaggcgttt gcgccggatc ccaagaatac gatactgttc gcggggtatc
     4081 aggtgcccgg cacgcgaggc gcgtcgttgg tggcgggcgc gcggacggtc aagatccatg
     4141 gagaatacgt gccggtcaac gcggaggtgg tggccatgaa taacgtgtcg gcgcacgccg
     4201 acgcggacga gatcatggcc tggctccggc aattccgctc gccgcctcgg aggacctttc
     4261 tcacccacgg cgaaccatcg gccgccgacg cgttgcgcca tcgcatcgaa gaggaactcg
     4321 ggtggtcctg ccgcgtgccg gagtaccgag aggtcgcgcg tctgacatga ccccgatcga
     4381 ccccccgccc gccgcgggcg aacaccgttt gcggatcaag cggctcggca tcgatacgta
     4441 ccaggagccc gtgatctaca tgcggcagga ctgcgacgtg tgccggtccg agggcttcga
     4501 agcccaatcc agagtggaaa tccgccgtga cggccggccc atcgtggcga cgttgaacgt
     4561 ggtcacctcc gacttgctgg cccgcaacga ggccgggctt tccgaagcgg cctggcgtct
     4621 gctcggagcc tcgcccgggg attcgatcac ggtgagacac ccaccgccgc tggaatcgct
     4681 gggtttcgtg cgggccaagg tgcacgggaa acggctggac gcgggcgcca tcaccgccat
     4741 cgtgcgcgac atcgcggcgg gacggtacgc ggacgtggag ctcgccgcgt tcctgaccgc
     4801 gggcgcggga tccggg
//
LOCUS       DBNGTT010000313         5519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2495738, whole genome shotgun sequence.
ACCESSION   DBNGTT010000313 DBNGTT010000000
VERSION     DBNGTT010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2495738"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1967)
                     /locus_tag="AC16BC_07055"
     CDS             complement(<1..1967)
                     /locus_tag="AC16BC_07055"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3241415.1"
                     /translation="MTDSIPGNEHTASGRIRHPDVDIRASAVADLSREGSTAIPLLME
                     ALGDTEWRVRKCAVEALVGMGWRAEMAQGLIAALASPGNAALRNAAAEAFVRFGGAAV
                     PAILSSLTGSEPDIQKFLVDILGDIGDRRVTTALIGLLGQTDENVAMAAVEALGKLRD
                     ARAVDPLSDILRSGRSLLQFSAVKALQEIGDGRAVEPLIGCLTRKPLERAALEALGRI
                     GDLRVLNPIVQSLRGGTAKVRHIAVRALIDLHDRMPPDAKTKVICRIREIYGESVAQY
                     LRECLRSDDPLIKRHAITLLGWMGDVQAVGELAAVYDEACKEELVAAFVRMHREGVPK
                     LVEILPTAPGGLREGIARALGEIGDRKAVHGLIQLATDVEGHVRQAAAVALGHLADPL
                     GVRVLLKLLEDQYHNVQDAAYRALIRLKGPVLIKRLLELMESPDAGLRCHVAKLLGVF
                     QVPEAQGRLMLDLKDPNPTVRRTALAALESLGGDMSEVFPVALSDEDPKVRLETVRIL
                     AKRPGRGNEDLLRPLLHDPDMWIRSEAIRLLVERGGDGIADTLLLLVNDPVGMIQIAV
                     CEAMGKLGVRGSLPALHRLLSSTDPDVKQAAVTAVGEIGGDEVAGQVLPLLDDSHWGV
                     RAAAAVALGRARVVQALPRLRELADRDPDQLV"
     gene            complement(1985..3088)
                     /locus_tag="AC16BC_07060"
     CDS             complement(1985..3088)
                     /locus_tag="AC16BC_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3241416.1"
                     /translation="MNIVCAQCGAAVASELSSLANQTVVVCPTCRSGLRVTIEAFAVP
                     AAVPAPPSALSAGDRDRVVVAIQGEATRELIGELLSGQGFEVLEAASGAEALTKVKET
                     RPAFVLVDVGMSDVMGFQVVDAVRKEPANAGVKIILVAAIHNKDRYRRQPDNLFGADD
                     YIERHQIETELIAKINALRAQSTPAKPVEAATPSATVDHNATRNDAQPAARSAAPGTG
                     ASLAAAVPNVQPAERPAPRPQPQAPTPPVAAVPPAVSRPPQQPLVVKEAAPTPAAAPE
                     PSAETPAQQAAFRLARIIVSDIALYNAKKVDDGVLNGTFFELLRTEIEEGRKLYEERK
                     GTDLPATTDLFQQTLEQFVATRRELLKRNRPAA"
     gene            complement(3085..5316)
                     /locus_tag="AC16BC_07065"
     CDS             complement(3085..5316)
                     /locus_tag="AC16BC_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR3241417.1"
                     /translation="MRLLKSPRRRLSSKVIATVLVTLAVGFIAIFVILQTKVRHDLIN
                     HEREKASLLAASIHLSFDKDMVAFRADMARHLVEDLKELPGIVRIQVIRGKDGLGVEQ
                     GFMDLKTIEEVKTRVPARSEWLVDHPNVVPNRAQGVETPEFRRAFERILADPMKAADE
                     YYFERINDREVLTYLRPLPNFQRCYLCHGSDHQLRGVLMISSVTDQMWNEVGANRRQL
                     LWGALGTMTVVGLLLQVSLRRGVFNPLHQVVERIKDVSDGEGDLTSRLDLTTRDEIGE
                     LAGGFNRFVEKLELIIREVSEISRTVSVAGRELARDATSVQSGVTSQTAGVESTLRSM
                     TDLHQAMNELGVGMESLSTMIEESTAATIEMASSTDEIARDADSLSGSASGTQRAVTT
                     LAESIRQVETALDALSRAATETAVSTRAISQSTTQIRANIQETVELSNRMGEHARSGQ
                     DCVGQTIEGINRIKTFSDEASSVIGRLRQRTEDIGTILVVIDEVAEQTNLLALNAAII
                     AAQAGEHGKGFAVVAGEIKELADRTATSTKEIHEIIAALQVEGASAVRAIADGATRVD
                     EGVRLSGAAKVALDRILESAKDSSSRILQIARETDQQMAGVQRVNAEMQTVTTQVAHI
                     VEVSHAQTRMLDEVEHISEQMQAMAHRVTRAAAEYAKGNRQVSHVIEMANQRVKDTLE
                     FVRKRRADSEAMVEAIRTIGRIGLENQDAMHRTAGAIDGLLGAASRLEEHVSRFKLTK
                     ENA"
     gene            complement(5316..>5519)
                     /locus_tag="AC16BC_07070"
     CDS             complement(5316..>5519)
                     /locus_tag="AC16BC_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR3241418.1"
                     /translation="GRLLGVIVDRVNDVLEVDSESFQDREQPGDGPTMCRGVCRVKED
                     LLLVIDLGAVLGQEEYAKLAEVV"
BASE COUNT          941 a         1917 c         1621 g         1040 t
ORIGIN      
        1 accagttggt caggatcacg gtccgcgagt tcacgcaacc gcggcaatgc ctggacgacc
       61 cgtgcgcgac cgagcgcaac cgccgccgcc gcgcgcacgc cccaatggga atcatccagc
      121 aacgggagca cctgcccggc cacctcgtcc cctccgatct cgcccacggc ggtcaccgcc
      181 gcctgtttga cgtcgggatc ggtcgacgac agcaaccggt gcagcgccgg gagcgatccc
      241 cggaccccga gtttccccat cgcttcgcac accgcgatct ggatcatccc caccggatca
      301 ttgaccaaca agagcagcgt atcggcaatc ccgtcgccgc ctcgttccac cagcagccgg
      361 atcgcctcgg atcgaatcca catgtcggga tcatgcagca atgggcggag caggtcttca
      421 ttgccccgcc ccggacgttt ggccagaatc cggaccgtct ccagacggac cttcggatct
      481 tcatcactca gcgcaacggg gaacacctcg ctcatgtcgc cccccagcga ctccagggcc
      541 gccagcgcgg tgcgtcgcac cgtggggttc gggtctttga gatccaacat cagtcgccct
      601 tgcgcctccg ggacctggaa cacgcccagc aacttcgcga cgtggcagcg gagcccggcg
      661 tccggactct ccatgagttc caagagccgt ttgatcagga ccgggccttt gaggcggatc
      721 aacgcccgat aggcggcgtc ctggacgttg tgatactgat cctcgagcag cttcagcaac
      781 acgcggacgc ccagcggatc ggccagatgg cccaacgcga cggccgccgc ctgcctgaca
      841 tggccttcga catcggtcgc caattgaatc aggccgtgga cggctttccg gtccccgatt
      901 tctccgagcg ctctcgcgat gccctcccgc aaacctcccg gagcggtcgg aaggatctcg
      961 accaatttgg gcaccccttc gcgatgcatg cggacaaagg ccgcgaccag ttcctctttg
     1021 caggcctcat cgtagacggc cgccaactcg cccacggctt gcacatcgcc catccacccc
     1081 aagagcgtga tcgcgtggcg cttgatgagc ggatcgtcgc tcctgagaca ttcgcgcagg
     1141 tactgggcaa ctgattcgcc gtatatctcc cggattcgac agatgacttt tgtcttcgcg
     1201 tccggcggca tgcggtcgtg caagtcgatg agcgcgcgca cggcgatatg cctcaccttg
     1261 gcggtgccgc cccggaggct ctgaacgatc gggttgagca cgcgcaggtc gccgatccga
     1321 ccgagggcct ccagcgcggc gcgctcgagc ggttttcgcg tgagacatcc gatgagaggc
     1381 tcgacggcgc ggccgtctcc gatctcttga agcgccttga ccgccgagaa ttgcagcaag
     1441 gatcgcccgg aacgcagaat gtcgctgagc gggtcaacgg ccctggcgtc acgaagtttg
     1501 ccgagcgctt cgacggccgc catggcgaca ttctcatcgg tctggcccag aaggccgatc
     1561 agggccgttg tgacacgccg atccccgata tcccccaaga tatcgaccaa gaacttctgg
     1621 atgtccggct cggagccggt gagcgagcta aggatcgcgg ggaccgccgc gccgccgaaa
     1681 cgcacgaacg cctcggccgc cgcattccgc agcgccgcgt tgcccggaga ggccagtgcg
     1741 gcaatcagcc cttgggccat ctccgctcgc cagcccattc ccaccaatgc ttccaccgcg
     1801 cattttcgga cgcgccactc cgtatcgccg agcgcctcca tgagcagcgg aatcgccgtt
     1861 gatccctcgc ggctcaggtc tgcgacggcg gatgcccgga tgtccacatc cggatgccga
     1921 attcgaccgc ttgccgtgtg ctcgtttcct ggaatcgaat cagtcacgat gttcgcctcc
     1981 accgttacgc cgccgggcgg ttccgtttca gaagctctcg gcgggtcgcg acaaattgct
     2041 caagggtctg ctgaaagagg tccgtggtgg ccggaagatc ggtccctttt cgctcctcat
     2101 acaatttacg cccttcctcg atctccgtac ggagcagttc gaaaaacgtc ccgttcagca
     2161 cgccgtcgtc caccttcttc gcgttgtaca gcgcgatatc cgagacgatg attcgagcga
     2221 ggcggaacgc cgcctgttgc gcgggagtct ccgccgacgg ctcgggggca gccgcgggag
     2281 tcggggcggc ctctttgacc acgagcggtt gctgcggtgg acggctcacg gccgggggga
     2341 ccgcggcaac gggcggcgtc ggcgcctggg gctgcggccg cggagccggt cgttcggctg
     2401 gctggacatt gggaacggcc gctgcaaggc tcgcgccggt tcccggcgcc gcggaccggg
     2461 ccgccggctg cgcgtcattc cgcgtcgcat tgtggtcgac cgtcgcggaa ggcgtggccg
     2521 cctccacggg cttcgcaggc gtcgactgtg cgcggagggc gttgattttg gcgatcaact
     2581 ccgtctcgat ctggtgacgt tcgatatagt cgtccgcgcc aaacagatta tcgggctgac
     2641 gtcggtaccg atccttattg tggatcgccg ccacgagaat gatcttcact ccggcgttcg
     2701 cgggttcttt ccgcaccgcg tcaaccactt gaaaccccat cacgtcggac atccccacgt
     2761 ccaccaagac gaacgcggga cgcgtctcct tgaccttggt caacgcctcc gcgccgctcg
     2821 ccgcttcgag aacctcgaat ccctgacctg aaaggagttc gccgatgagc tcgcgcgtgg
     2881 cttcgccctg aatggccacc actacacgat cccgatcccc ggccgacagc gcggaaggcg
     2941 gagcgggaac cgccgccgga accgcgaacg cctcgatcgt cactcgcagg ccgctccgac
     3001 aggtcggaca cacgactacg gtctgattgg cgagggacga caattcagag gctactgccg
     3061 cgccgcattg tgcgcacacg atgttcatgc gttctccttc gtgagtttga accgggaaac
     3121 atgctcctcc agtcgcgacg ccgcgcccaa caagccgtcg atcgccccgg ccgtccgatg
     3181 catggcgtct tgattctcca gcccgatccg tccgatcgtg cggatggcct ccaccatggc
     3241 ctcactgtca gcgcgtcgct tccggacgaa ctccagcgtg tcctttaccc gctggttggc
     3301 catctcgatg acgtgactga cctgtcgatt ccctttggcg tactcagccg ccgcacgggt
     3361 cacgcggtgc gccatggctt gcatttgttc gctgatatgc tccacttcat cgagcatccg
     3421 cgtctgcgcg tgggatacct cgacgatgtg cgcgacctgg gtggtcacgg tctgcatttc
     3481 ggcgttcact cgttgcacgc ccgccatctg ctgatcggtt tcccgcgcga tctgcagaat
     3541 acggctcgat gagtccttcg cgctctccag aattctatca agggcgacct tggcggcacc
     3601 cgagagccgc accccctcgt ccacccgggt cgcgccgtcc gcgatcgccc gcaccgcgct
     3661 ggcgccctcg acctgcaacg ccgcgatgat ttcgtggatt tccttggtgg atgtcgcggt
     3721 ccggtccgcc agttctttga tctcgccggc cacgaccgcg aagcccttgc cgtgttcgcc
     3781 ggcctgagcg gcgatgatcg cggcattcag cgcgaggaga ttcgtttgtt cggccacctc
     3841 gtcgatgacg accaagatgg ttccgatgtc ttccgtgcgc tgccggagcc gcccgatcac
     3901 ggacgacgct tcgtcggaga acgtcttgat ccggttgatg ccttcgatgg tttgcccgac
     3961 gcagtcttgt ccgctccgcg cgtgttcgcc catccggttc gacagctcga ccgtttcctg
     4021 gatgttcgcg cgaatttggg tcgtcgactg ggaaatggcc ctggtggaca cggcggtctc
     4081 ggtcgcggct cgcgaaaggg cgtccagggc cgtctcgacc tgccggatcg actcggccaa
     4141 cgtcgtgacg gcgcgttgcg tgcccgacgc ggaccccgag agcgagtcgg cgtcacgcgc
     4201 gatctcgtcc gttgacgacg ccatctctat cgtggccgcc gtactctcct cgatcatggt
     4261 cgacaatgat tccatcccga ctccgagctc attcatggcc tggtgcagat cggtcatcga
     4321 gcgcagggtg gattccaccc cggcggtctg tgaagtcact ccgctctgca ccgacgtggc
     4381 gtcccgcgcc aactcccggc ccgcaaccga aaccgtccgg ctgatctcgg agacttcccg
     4441 gataatcagt tcgagttttt ccacgaaacg attgaatcct ccggccagtt ctccgatttc
     4501 gtcccgggtg gtcaggtcga gccggctcgt caggtcaccc tcgccgtcgc tgacgtcctt
     4561 gatgcgctca accacctgat ggagcggatt aaagacgccc cgacggagcg aaacctgcag
     4621 cagcaaaccg actacggtca tcgtgcccag cgcgccccac aacaactggc ggcgattcgc
     4681 ccccacctcg ttccacatct ggtcggtgac ggacgaaatc atgaggaccc cgcgcagttg
     4741 atgatccgat ccgtgacaca gataacagcg ctggaaattc ggaagcggtc gcaggtacgt
     4801 caacacctcg cggtcattga tccgctcaaa gtaatactcg tcggcggcct tcatcgggtc
     4861 cgccaagatg cgctcgaacg cgcggcgaaa ctccggtgtt tcaacgcctt gcgcgcgatt
     4921 cggcacgaca ttcgggtgat ccaccagcca ctccgaacgc gccggcactc gcgtcttcac
     4981 ttcctcgatg gtcttgagat ccatgaagcc ctgctccacc cccaagccgt ccttgccgcg
     5041 aatcacctgg atgcgcacga tgcccgggag ttccttgagg tcctcgacca ggtgtcgagc
     5101 catatccgcc cggaacgcca ccatgtcctt gtcgaagctc aagtggatcg acgccgcgag
     5161 cagcgacgct ttctctcgtt catgattgat caggtcatgg cgcaccttgg tctgcaagat
     5221 gacgaagatc gcgatgaacc cgaccgccag agtgaccagc accgtggcga tgactttcga
     5281 cgacaaccga cgacgtgggg acttcaacaa ccgcatcaga ccacctccgc tagtttcgcg
     5341 tattcctcct gccccaacac cgcccccaga tcgatcacga gcaacaagtc ttccttgacc
     5401 cgacagacgc cgcgacacat cgtcggtccg tcgccgggct gttctcgatc ctgaaagctc
     5461 tcgctgtcaa cctccaggac atcgttcacc cggtccacga tgacccccag gagccggcc
//
LOCUS       DBNGTT010000314         9918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1218849, whole genome shotgun sequence.
ACCESSION   DBNGTT010000314 DBNGTT010000000
VERSION     DBNGTT010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1218849"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2342
                     /gene="lon"
                     /locus_tag="AC16BC_07075"
     CDS             <1..2342
                     /gene="lon"
                     /locus_tag="AC16BC_07075"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3241419.1"
                     /translation="VFPPMVLPLFVGREMSIKAIDEALAGNRLVLLVAQRALDVENPQ
                     PKDIYSIGTVGMIMRMLKLPDGRIKILVQGLSRARVTDYEQTTPFYVVQTEKVAEVQT
                     EEVGLEVEALMRTVKEQLEKIIGFGKLLLPEIMVVIENLDEPGRLADMIAANLGLKVD
                     VAQDVLEILDPVARLRRVNEILNKEIELLSMQQKIQAEAKGEMDRSQREYFLREQLKA
                     IQKELGEVDERAEEIAEIRKRIKKSKMPEKVFKEADKQLKRLEKMHPDSAEAATIRTY
                     LEWMVEIPWSKKTKDNLDIPSAEKVLNADHYDLEKVKERILEFLAVRKMKEKMKGPIL
                     CFLGPPGVGKTSLGKSIARSLGREFVRMSLGGVRDEAEIRGHRRTYVGSLPGRIVQGI
                     KQAGSNNPVFMLDEVDKIGMDFRGDPAAALLEVLDPEQNHAFTDHYLGVPFDLSNVMF
                     ITTANLLDPIPGPLRDRMEVIDISGYTEEEKVGIAKNYLIPRQLTEHGITEKHVDLTE
                     AALRMIITQYTREAGVRNLEREIANVFRKVARKVAEGKVKQYPITPKTLQSYLGIPKH
                     LPEAEQEHDEVGVTTGLAWTESGGDIIYIEATTMKGKGAITLTGHLGDVMKESAHAAL
                     SYIKSRQHEIGIKPDVFGKLDVHVHVPAGAIPKDGPSAGITMATALASAFTETPVRHD
                     VAMTGEVTLRGRVLPIGGLKEKLLAAKRAGMKTVIIPKRNEKDLEDVPKHVKQGLQLV
                     FAETMDDVLPVALKGFRMIKTRSPRPRSGRVNRALSINA"
     gene            2428..3453
                     /gene="thiL"
                     /locus_tag="AC16BC_07080"
     CDS             2428..3453
                     /gene="thiL"
                     /locus_tag="AC16BC_07080"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR3241420.1"
                     /translation="MSRELSRIGEFGLIARLTKLCPTSSPQLRLGVGDDAAVVRPRSN
                     TELLVTTDVLVEGVDFDRANTPFDRLGEKALAANLSDIAAMGGRPAVFFVSLSLPSRA
                     RVREAEALYRGLGRCARRYGVTLGGGDVSAAPCWSIAVTVLGEVARGRALTRAGARAG
                     DVLCVTGTLGDSIAGLALLKRRGLMPRGLARSNARRLLDRHQRPVPRLVIGQRLAEQG
                     LATAAIDLSDGLAADVRHLCEASKVGVEIDLSALPMSTALRRYAEAVRRDPIGFALGG
                     GEDFELLVAIPPKHLARAQRLVHRAARLTVIGRVTSARQGLTVIDATGRRRPLPAVGF
                     EHFRSPG"
     gene            complement(3679..5256)
                     /locus_tag="AC16BC_07085"
     CDS             complement(3679..5256)
                     /locus_tag="AC16BC_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR3241421.1"
                     /translation="MAQRLGELLCAHARLTPQALGRGLEEQRRTGGLLGEQLIALNLL
                     EPAALSAALAQQTELQRIEYEQAVPTLAADAARGRSAEGDAAPPLVRLVDALIAYGVV
                     RQASDIHIEPTAEATLVRYRVHGLLASAFRLPKGLHPALVSRVKILAALDIAERRLPQ
                     DGAVRGRHAGHAVDLRISSLPSQHGETVVIRVLDHSRQLLNLNALGLTKRETDAVHAI
                     LARRKGIILVTGPTGSGKTTTLYAMLNAIRDGTTNIVTVEDPIEYSITGVNQVQVNVD
                     SGLTFARALRAILRQDPDVVLIGEIRDGETAEIAYRAAMTGHLVLSTLHTNDAPATIA
                     RLTDLGVPRYLVAAHTGGILAQRLVRALCPACRVQGRPALADALHLGLPASAADDATW
                     WLPRGCDACNGGGYRGRLSLFESLLPSRELREHIATGAHEQSLRMTALRTGMVSLLAA
                     GLAKAGEGLTSLEELARVLEPDDGAPPLCDACGKIRPGPTPYCPLCGARATGSCAGCG
                     DALAATWTHCASCGTAR"
     gene            5439..8018
                     /locus_tag="AC16BC_07090"
     CDS             5439..8018
                     /locus_tag="AC16BC_07090"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3241422.1"
                     /translation="MSTERLSGRLPDAVTPQAYDLTLDVRPEEGRFSGRVRIALRLTS
                     PTREIALHAQDLVVSEAAIVTGERRRPLTAALDAEATRLTLASPDTLPAGQASLELTF
                     SGSLNRQMKGLYEARAVVDGLDERYAFTQFEPTDARRCFPCFDEPALKASFRVQVTAP
                     AHLAVLSNMPALSETREGSSKTVRFAETPVMSTYLLAVAVGRLAAKRRTIAGTDVAVW
                     ALPNDLTLADFALDVTAATLPLMNDYFGLPYPYPKLDLIAVPDFAMGAMENWGAIFFR
                     DSRLLVDPARASTATQRVVANVIVHEIVHQWFGNLVTMRWWDDLWLNEAFATWLACKI
                     VDQWRPEWRSWDEFQLEKHVPLALDALDSTRPIVAGADTSAEIEALFDPLTYEKGAAV
                     LRMFEQFLGEDAFRAGIRAYMKQHQFGNTVAADLWRALEAASGRPVGDLARDWLTQAG
                     YPLVTVRATGADRRTIQLTQRRWSAHGRGRGATGRWRIPVVIRYEDRRGLHAHRALLE
                     QDQAAVTLPAEGAVTWVYGNGAESGFYRVQHEGDLHAALLSGGWARLDAAERVGLLNH
                     AWAAAEAGDAPINVLMDTILACRGDGTRVVVEAMTGYLDTLAERIVPPEARPTLARLT
                     AELAAPVWDRLGWQGRGAEDDEARLARAAAMWLMAVVAGEPAVCREASRRADAYLADA
                     SSLEPTLVANVLRIGARLGDATRFEAYLARFRAARTPEDRDRLLSALADFPDRALLDR
                     FLAFLCTDEVRGQDVWKPFRTLLGNPSTQDPTWRFVKATWLLLREKAGPVGSGRIIQA
                     TRGLWRTDWRGDVAEFFSRPEHRVESAAKALDQTLEFMDVGIAFKSAQVKPLSRWLRG
                     RFP"
     gene            complement(8041..>9918)
                     /locus_tag="AC16BC_07095"
     CDS             complement(8041..>9918)
                     /locus_tag="AC16BC_07095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3241423.1"
                     /translation="LGRATGWGFAVSIIAALALVIGGIQWVRRNRAPVGLRSARTATH
                     ADGRHRLEDALRKSEKRLAEAQRLAHLGSWELDLAKNHLWWSDEVYRIFEIDPDQFGA
                     SYEAFLETVHPEEREFVHHAYVESVQNKTPYDLAHRLLMKDGRVKYVHECCETDYDAD
                     GRPVRSIGTVLDVTERKLADEAIRQLAFHDSLTGLPNRAMLHDQLAKAIEFSERRRTT
                     FAFLIMDLNDFKDINNTLGHHHGDLMIKEIGPRLQGVLRQSDTIARLGGDEFGLLLPD
                     TDLDGALGVAKKIHRALEAPFKIEGLSILVEASIGVAVFPTHGATASAVIQHADVAMY
                     AAKHTRTGTAVYAADRNHDSPRRLALMAELRYAIERDELTLYYQPKINLQTRRVIGVE
                     ALVRWNHPNRGLIPPDEFITLAERSGLIRPLTLRVLDMALLQARAWAQAGFQLRVAVN
                     LSARNLQDAQLPEQILELLGRTGTSPDRLELEITESTIMADPNRSLAVLTRLHDMGIA
                     LAIDDFGTGYSSLSYLKRLPISAVKIDKSFVQNLPSNDNDAVIVRSTIELAHNLDLQV
                     VAEGVETAYIWDRLVALGCDAAQGFYMSQPMPADALNQWFVESSWQVGLVPPGDATQA
                     A"
BASE COUNT         1643 a         3140 c         3482 g         1653 t
ORIGIN      
        1 tggtcttccc gcccatggtg ctcccgctgt tcgtggggcg ggagatgtcg atcaaggcga
       61 tcgacgaggc gctcgcgggc aatcgcctcg tgttgttggt ggcgcagcgc gcgctggacg
      121 tggagaaccc gcagcccaag gacatctact cgatcggcac ggtcggcatg atcatgcgga
      181 tgctgaaact gcccgacggc cgaatcaaga tcctggtcca gggactgtcc agggcgcgcg
      241 tgacggacta cgagcagacc acgccgtttt acgtggtgca gaccgagaag gtggccgagg
      301 tgcagaccga ggaggtcggt ctggaagtcg aggcgctcat gcgcacggtc aaggagcagc
      361 tcgaaaagat catcgggttc gggaaattgc tgttgcccga aatcatggtc gtgatcgaga
      421 acctcgacga gccgggccgc ttggccgaca tgatcgcggc caacctcgga ttgaaggtgg
      481 acgtggcgca ggacgtgctg gagatcctgg acccggtcgc gcggctgcgg cgcgtgaacg
      541 agatcctcaa caaggagatc gagctgctct ccatgcagca gaagatccag gccgaagcca
      601 agggcgagat ggatcgcagc cagcgcgagt acttcctgcg cgagcagctc aaggcgatcc
      661 aaaaagaatt gggcgaggtc gacgagcgcg ccgaggagat cgcggagatc cgcaagcgga
      721 tcaagaaatc caagatgccg gagaaagtct tcaaagaggc ggacaagcag ctcaagcgcc
      781 tggagaagat gcacccggat tcggccgaag ccgccaccat ccgcacgtat ctggaatgga
      841 tggtggagat tccctggagc aagaaaacca aggacaacct ggacatcccg tccgcggaaa
      901 aagtgctcaa cgcggatcac tacgatttgg agaaggtcaa ggagcgcatc ctggaatttc
      961 tcgccgtgcg caagatgaag gagaagatga agggcccgat cctgtgcttc ctggggcctc
     1021 ccggcgtggg caagacgtca ttgggcaaat ccatcgcccg ctcgttgggg cgcgagttcg
     1081 tgcgcatgtc gctgggcggg gtgcgcgacg aggccgagat ccgcgggcat cgccgcacct
     1141 acgtgggctc gctgccgggc cggatcgtcc agggcatcaa gcaggccggt tcgaacaacc
     1201 cggtgttcat gctggacgaa gtggacaaga tcggcatgga ttttcgcggc gatcccgcgg
     1261 ccgcgttgct ggaagtgctg gacccggagc aaaaccacgc gttcaccgac cactacctgg
     1321 gcgtgccgtt cgatttgtcg aacgtcatgt tcatcaccac cgcgaatctg ctcgacccga
     1381 tccccggtcc gctccgcgac cgcatggagg tcatcgacat ctcgggctac accgaggagg
     1441 aaaaggtcgg gatcgccaag aactatctga tcccgcggca attgaccgaa cacggcatca
     1501 ccgaaaaaca cgtggacctg accgaagccg cgctgcgcat gatcatcacc cagtacacgc
     1561 gcgaggcggg cgtgcggaac ctggaacgcg agatcgccaa cgtgttccgc aaggtcgcgc
     1621 gcaaggtggc ggagggcaag gtcaagcagt acccgatcac gcccaagacc ctgcagtcgt
     1681 acctgggcat tcccaaacac ttgcccgagg cggaacagga acacgacgag gtcggcgtga
     1741 ccacgggcct ggcctggacc gaatccggcg gcgacatcat ctatatcgaa gccaccacca
     1801 tgaaaggcaa gggcgcgatc acgttgaccg ggcacctggg cgacgtgatg aaggagtcgg
     1861 cgcacgccgc gctcagctac atcaagtcgc gccagcacga gatcgggatc aagcccgacg
     1921 tgttcggcaa actcgacgtg cacgtgcacg tgcccgcggg cgccattccc aaggacggcc
     1981 cgtccgcggg catcaccatg gccacggccc tggcgtccgc gttcaccgag accccggtgc
     2041 gccacgacgt ggccatgacc ggcgaggtga cgttgcgcgg ccgcgtgctg cccatcggcg
     2101 ggctcaagga aaagctcctg gccgccaaac gcgcgggcat gaagaccgtg atcatcccga
     2161 agcgaaacga gaaggacctg gaggacgtgc ccaaacacgt caagcaggga ctgcagctcg
     2221 tgttcgcgga aaccatggat gatgtgttgc cggtggcgtt gaagggattt cgcatgatca
     2281 agacgcgctc accgcgccct cgttccggcc gcgtgaaccg cgcgctttcg atcaacgcat
     2341 gagcgctgac ttgccacccg atgtctgaaa tcccttcgtg ccccctttga aaaagggggg
     2401 atggggggat ttgctttttg cgcatccgtg agccgtgaac tttcccggat cggtgagttc
     2461 gggctgatcg cgcgcctgac aaaactctgc ccgacctcct ctccgcagct gcgtctcggc
     2521 gtcggtgacg acgcggcggt cgtcaggccg cgatccaata ccgaactgct cgtgaccacc
     2581 gatgtgctgg tcgagggcgt ggattttgac cgggccaaca cgccgttcga ccggctgggg
     2641 gagaaggcgc ttgcggccaa tctcagcgac atcgcggcca tgggcgggcg tcctgccgtc
     2701 tttttcgtgt ctctgtcact tccgtctcgc gcccgggtgc gtgaggccga ggcgctctac
     2761 cggggacttg gtcgctgcgc gagacggtac ggggtgacgc tcgggggcgg ggacgtgtcc
     2821 gcggcgccgt gttggtccat cgcggtgacc gtgttgggcg aggttgcgcg cgggcgggcg
     2881 ctcacgcgcg cgggcgcgcg ggccggcgat gtgttgtgcg tgaccgggac attgggggac
     2941 tcgatcgcgg ggctggccct gctcaaacgg cgcgggctga tgccccgcgg cctggctcga
     3001 tccaacgcgc ggcggttgct cgaccgccat cagcgtcccg tcccccgact cgtgatcgga
     3061 caacgcttgg cggagcaggg tctggccacg gccgcgatcg atctctccga cggtctggcc
     3121 gctgacgtgc ggcacctgtg cgaagcgagc aaggtcggcg tcgagattga cctgtccgcg
     3181 ctaccgatgt cgaccgcgtt acgtcggtat gccgaagcgg tccggcgcga cccgatcggt
     3241 tttgccttgg gcggcggaga ggacttcgaa ttactggtcg cgatcccgcc aaaacacctc
     3301 gcgcgcgcgc agcgtctggt ccaccgcgcc gcgcggctca cggtcatcgg ccgggtgaca
     3361 tcggctcggc agggattgac cgtgatcgat gcgacgggcc ggcggcgtcc gctgccagca
     3421 gtggggttcg agcatttccg gagtcccggg taagtacgga catccagata ttctgcgagg
     3481 tcgctgaaga actggtggag gggctgcggc ccgctcaaaa agctccagct gcaaggccgc
     3541 agggaggaga aaaaccggag cgtactgggt cgtacgtgag gatttttcga cgaccgagaa
     3601 tccgcaccgc gccgaaggcg tgggatggaa atagtcgctt ccctacagat ggcgaagcaa
     3661 cgccgcagat ggactttttc agcgggccgt gccgcaggac gcgcagtgcg tccacgtcgc
     3721 cgccagcgca tccccgcacc ccgcgcacga accggtggcg cgcgcaccgc agagcgggca
     3781 atagggcgtc ggccccggcc tgatctttcc gcacgcgtcg cacaacggcg gcgcgccgtc
     3841 gtccggctcc aacacccgcg ccaattcctc cagcgacgtc aggccctccc cggcctttgc
     3901 caatcccgcg gcgagcagcg agaccatccc ggtccgcagc gcggtcatgc gcagactctg
     3961 ttcgtgtgcg ccggtggcga tgtgttcgcg caattcgcgc gaggggagca gggactcgaa
     4021 caggctgagc cgcccgcgat agccgccgcc gttgcacgcg tcgcagccgc gcggcagcca
     4081 ccaggtcgcg tcgtcggccg cggaagcggg caggcccagg tgcagcgcgt ccgcgagcgc
     4141 gggccgtccc tggacacgac acgcggggca gagcgcgcgc accaggcgtt gcgccaggat
     4201 gcccccggtg tgcgcggcca ccagatagcg cggcacgccc aggtcggtca gccgggcaat
     4261 ggtggcgggc gcgtcgttgg tgtgcaacgt ggagagcacg agatgtccgg tcatggccgc
     4321 gcggtacgcg atctccgcgg tctcgccgtc ccgaatctcc ccgatcagca ccacgtcggg
     4381 gtcctggcgc aggatcgcgc gcaacgcgcg ggcaaaggtc agcccgctgt ccacgttgac
     4441 ctggacctga ttgaccccgg tgatggagta ttcgatcggg tcttccaccg tgacaatgtt
     4501 ggtggtgccg tcccggatcg cgttgagcat ggcgtacagc gtggtggtct tgccgctgcc
     4561 ggtcgggccg gtcaccagga taatgccctt gcggcgcgcg agaatggcgt gaaccgcatc
     4621 cgtctcgcgc ttggtcagtc ccagcgcgtt gaggttcagc agttgccggg aatgatccag
     4681 cacgcggatc accacggttt cgccgtgttg gctgggcaac gaggagatgc gcaaatccac
     4741 ggcgtgcccg gcgtggcggc cgcgcaccgc gccgtcctgc ggcaatcgtc gctccgcgat
     4801 gtcgagcgcg gcgagaattt tcacgcgcga caccaacgcg gggtgcagcc ccttgggcaa
     4861 ccggaacgcg gacgcgagca ggccgtggac ccggtaccgc accaacgtgg cctcggcggt
     4921 cggttcgata tggatgtcgc tggcctggcg caccacgccg tacgcgatca acgcgtcgac
     4981 cagccgcacc agcggaggcg cggcgtcgcc ctcggccgac cgtccgcgcg ccgcatccgc
     5041 ggccagggtg gggacggcct gttcgtactc gatgcgctgg agttcggtct gttgtgcgag
     5101 cgcggcggag agcgcggcgg gctccagcaa gttgagggcg atgagctgtt cacccaggag
     5161 gccgccggtc cggcgttgtt cctccagtcc tcgcccgagc gcctgcgggg tcaggcgggc
     5221 gtgcgcgcac aagagttcac cgaggcgttg cgccatggtt acctccggat ggagagccgc
     5281 gtcccgtggg gacatgcgac ggggcgcgag gctgtcggcc cggcatccta cgcctgtacc
     5341 gaggcggaat caagcgccct cgcgggtcag cccaaatcag tgtttgctcg gtgcaacgtg
     5401 ttttgcgata atgcgcaagt tgtcgaggga ccgactgcgt gagcactgag cggctgtcgg
     5461 ggcggttgcc ggatgcggtc acgccccaag cctatgacct gacgttggac gtacggcccg
     5521 aggaggggcg attctccggg cgcgtgcgta tcgcgctccg cctgacgtcg cctacccgcg
     5581 agatcgcgct gcacgcgcag gacctcgtcg tgtccgaggc cgcgatcgtg acgggcgagc
     5641 gtcggcgccc gctgacggcg gcgctcgacg cggaggcaac ccgcctcacg ctcgcctctc
     5701 ccgacaccct ccccgccggc caagcctcgc tcgaattgac cttttcgggg tcgctcaatc
     5761 gtcagatgaa ggggttgtac gaagctcgcg ccgtcgtcga cggcctcgat gaacggtacg
     5821 cgttcacgca gttcgaaccc acggacgcgc ggcggtgttt tccgtgtttc gacgagcccg
     5881 cgctcaaggc gtcgttccgg gtgcaggtga cggctccggc ccacctggcc gtgctctcca
     5941 acatgcccgc gttgtccgag acccgcgagg ggtcgagcaa aaccgtgcgg ttcgccgaaa
     6001 ccccggtcat gtccacgtat ctgctcgcgg tggcggtggg ccggttggcg gccaagcgcc
     6061 ggaccatcgc gggaaccgac gtggcggtgt gggcgctgcc gaacgacctg acgctcgcgg
     6121 acttcgcgct ggacgtcacc gcggccacgc tgccgttgat gaacgactac ttcggcctgc
     6181 cgtaccccta tcccaaattg gatctgatcg cggtgccgga cttcgccatg ggcgcgatgg
     6241 aaaactgggg cgcgatcttt ttccgagatt cgcggctgct ggtcgatccc gcccgcgcct
     6301 cgaccgccac gcagcgcgtg gtggccaacg tgatcgtcca cgaaatcgtc caccagtggt
     6361 tcgggaatct ggtgaccatg cggtggtggg acgatctctg gttgaacgag gcgttcgcga
     6421 cgtggctggc gtgcaagatc gtggatcagt ggcggcccga gtggcggtcg tgggacgaat
     6481 tccagctcga gaaacacgtg cccctggcgc tcgacgcgtt ggacagcacc cgcccgattg
     6541 tggcgggcgc ggacacgtcg gcggagatcg aggccctgtt cgacccgttg acctacgaga
     6601 agggcgcggc cgtcttgcgg atgttcgagc agttcctggg ggaagacgcg ttccgcgcgg
     6661 gcattcgggc gtacatgaag caacaccagt tcgggaacac ggtggcggct gatctatggc
     6721 gcgcgctgga ggccgcgtcg ggacggcccg tgggggatct ggctcgggat tggctgacgc
     6781 aggcgggcta tcctctggtc accgtgcgcg ccaccggggc cgaccgccgg accatccaac
     6841 tgacgcagcg acgttggtcg gcccacggac gcgggcgcgg cgcgaccggt cgctggcgga
     6901 ttccggtggt catccggtac gaggatcgcc ggggtctcca cgcgcaccgc gccctgctcg
     6961 aacaggacca ggcggccgtg accctgccgg cggagggcgc ggtgacgtgg gtgtacggga
     7021 acggcgcgga gtcgggcttt taccgggttc agcacgaggg cgacctgcac gcggcattgt
     7081 tgtccggcgg ttgggcccgg ctggatgcgg ccgaacgcgt gggcctgctc aatcacgcgt
     7141 gggccgcggc cgaagccggc gacgcgccga tcaacgtcct gatggacacg atcctcgcgt
     7201 gccggggaga cggcacgcgg gtggtggtgg aagcgatgac cgggtatctg gacacgctgg
     7261 cggagcgcat cgtcccgccg gaggcgcggc cgacgctcgc gcgcctcacc gcggagctgg
     7321 ccgcgccggt ctgggatcga ctgggctggc aaggccgcgg cgccgaggac gatgaagcgc
     7381 gattggcccg cgccgcggcg atgtggctca tggcggtggt ggcgggcgag cccgcggtct
     7441 gccgcgaggc gagccgccgc gcggacgcgt atctggcgga cgcctcgtcg ctggagccca
     7501 cgctggtcgc gaacgtgctg cgcatcgggg cgcggctggg agacgcgacg cggttcgagg
     7561 cgtatctggc ccgcttccgc gccgcgcgca cgccggaaga tcgtgaccgg ctgctgagcg
     7621 cgctcgccga cttcccggac cgcgcgctgc tcgatcggtt tctggcgttt ctgtgcacgg
     7681 acgaggtgcg cggtcaggac gtgtggaagc cgtttcgcac gttgctgggc aatccgtcga
     7741 ctcaggatcc gacgtggcgg ttcgtcaagg cgacgtggct gctcctgcgg gagaaggcgg
     7801 ggccggtcgg gtcgggccgc attatccagg cgacccgcgg actgtggcga acggactggc
     7861 gcggcgacgt ggccgaattt ttcagccggc ccgaacaccg cgtcgaatcc gccgccaagg
     7921 cgctcgatca aaccctggag ttcatggacg tggggattgc gttcaagtcg gcgcaggtca
     7981 aaccgctgtc gcgctggctg cgcggacgat tcccgtgacc cgtgaggcgg cgcgtgggcg
     8041 tcaggccgcc tgcgtggcgt ctcccggcgg gaccaagccg acctgccagg acgactccac
     8101 gaaccattgg ttgagcgcgt cggccggcat cggctggctc atgtagaacc cttgcgccgc
     8161 gtcgcacccc aaggccacca atcgatccca gatatacgcg gtctcgacgc cttcggcgac
     8221 gacctgcagg tccagattgt gggcgagctc gatggtggag cgcacgatga ccgcgtcgtt
     8281 gtcgttggac ggcaggttct gcacgaacga cttgtcgatc ttcaccgctg agatgggcaa
     8341 ccgcttgaga tacgagaggg aggaatagcc ggtcccgaag tcatcgatgg ccaacgcgat
     8401 gcccatgtcg tgcagccggg tgagcacggc gagcgatcgg ttcggatcgg ccatgatcgt
     8461 gctctcggtg atttccaatt ccaaccggtc cggcgaggtg ccggtccggc ccaacaactc
     8521 gaggatctgt tcaggcagtt gcgcgtcctg gaggttccgg gccgagaggt tgaccgcgac
     8581 ccggagttga aagccggcct gcgcccacgc gcgcgcctgc agcagggcca tgtcgaggac
     8641 ccggagcgtc aacggccgga tcaggccgct gcgttcggcc aaggtgatga actcgtccgg
     8701 cgggatgagc cctcggttgg gatggttcca gcggaccaag gcctccacgc cgatgacgcg
     8761 tctcgtctgc aggttgatct tcggttgata atacagagtc agttcgtcgc gctcgatcgc
     8821 gtagcgtagt tccgccatca acgcgagccg tcgagggctg tcatggttgc ggtccgcggc
     8881 atacacggcg gtcccggttc gggtgtgttt ggccgcgtac atcgcgacgt ccgcgtgctg
     8941 aatgacggcg gaggccgtgg cgccgtgggt cggaaacacc gcgaccccga tgctggcttc
     9001 gaccagaatg ctgaggcctt cgattttgaa cggggcctcg agcgcccggt ggattttttt
     9061 cgccaccccg agggctccgt ccagatccgt gtcgggcaac agcaggccga attcgtcccc
     9121 tcccaggcgg gcgatcgtgt ccgattggcg gagcacgccc tggagccgcg gcccgatttc
     9181 cttgatcatc aggtcgccgt ggtggtgtcc cagcgtgttg ttgatgtcct tgaagtcgtt
     9241 gaggtccatt atcaggaacg cgaacgtggt gcgccgccgt tcgctgaatt cgatggcttt
     9301 cgccagttgg tcgtggagca tcgcgcggtt gggcaagccc gtgaggctgt cgtggaacgc
     9361 cagttgccgg atcgcttcat cggccagttt gcgttcggtg acgtccagga cggtgccgat
     9421 cgaacgcacc ggtctgccgt cggcgtcgta gtcggtctcg cagcattcgt ggacgtactt
     9481 cacccggccg tccttcatca acaatcggtg ggcgaggtca tacggcgtct tgttctgcac
     9541 cgattcgacg taggcgtggt ggacgaactc ccgctcctcc ggatgcaccg tttccaggaa
     9601 cgcttcgtac gacgcgccga attggtcggg atcaatttca aagatccgat acacctcgtc
     9661 ggaccaccac agatggttct tggccaggtc aagctcccag ctgccgagat gggcgagacg
     9721 ctgggcttcg gcgagtcgtt tctcgctctt gcgaagcgcg tcctcgaggc gatgtcggcc
     9781 gtcggcatgc gtcgcggtgc gggccgagcg aagccccacc ggcgcacggt ttcgccgcac
     9841 ccactggatg ccgccgataa ccagggcgag cgccgcgatg atcgagacgg cgaagcccca
     9901 gcccgtggct cggcccag
//
LOCUS       DBNGTT010000315         5713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1347410, whole genome shotgun sequence.
ACCESSION   DBNGTT010000315 DBNGTT010000000
VERSION     DBNGTT010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1347410"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /gene="glgP"
                     /locus_tag="AC16BC_07100"
     CDS             <1..597
                     /gene="glgP"
                     /locus_tag="AC16BC_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR3241424.1"
                     /translation="MTQYKRADLVFSDLKRLAQIGGGRLQIVLAGKAHPQDAQGQRIL
                     ERLFKAMEALRTDVPVVFLPDYNMDMALTIVSGCDLWLNTPCRPLEASGTSGMKATHN
                     GVPNFSVLDGWWIEGHVEGVTGWSIGPPPRESAPADVDDRRDAQDLYDKLERVILPLY
                     TQDRQGWIAVMKHAIGKNASYFNSHRMMRRYVTDAYLR"
     gene            638..1138
                     /locus_tag="AC16BC_07105"
     CDS             638..1138
                     /locus_tag="AC16BC_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241425.1"
                     /translation="MNAPSPFDWLKDLCTHFEWLHGSAQALADGRPIAPSSTIPDHAV
                     GEIMRAFFRIQDTMVAQQTERDALKTAVESRDAELASLHAEADAVRRELVETRAALGE
                     TRRTLEDRQRGWEEAEAKWQHERRVMDERLAGLEQRVRDVTGRLTEHARTLNAVAADL
                     ASSASS"
     gene            1221..1583
                     /locus_tag="AC16BC_07110"
     CDS             1221..1583
                     /locus_tag="AC16BC_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241426.1"
                     /translation="MARLRRLMPSASSTHLSRLANKVRRFVLCLVASDYVQEQATLRQ
                     GECVGCGRCCKLVFQCPFLGGTDENPRCLIYNVARPNQCAAFPIDERDLADVNYLCSY
                     SFAQPAPQPQQLLSLVYE"
     gene            1633..2556
                     /locus_tag="AC16BC_07115"
     CDS             1633..2556
                     /locus_tag="AC16BC_07115"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR3241427.1"
                     /translation="MQDRIRTILNHYSNENAGTKTNLARLLNHGALAGTGKLVILPVD
                     QGFEHGPARSFAPNPSGYDPAYHFRLGIDAGCNAYAAPLGFLEAGAAEFAGDIPLILK
                     LNNHDSLHDEKDPLPSQTGSVRDALRLGCVAVGYTIYPGSSRCQTMYEQLRAIAEEAK
                     AVGLAVVVWSYPRGSGLSKEGETAIDVVAYAAQIAAQLGAHIIKVKLPTEHVEQAAAK
                     KVYESNRIPITTLTDRVRHVVQSAFNGRRIVIFSGGAKEDDERVFTEVRAIRDGGGFG
                     SIIGRNSFQRAHSDALKFLDTIMKIYGGKIS"
     gene            complement(2705..4171)
                     /locus_tag="AC16BC_07120"
     CDS             complement(2705..4171)
                     /locus_tag="AC16BC_07120"
                     /inference="COORDINATES: protein motif:HMM:NF045061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3241428.1"
                     /translation="MNGRLCRVVWLGASALIACTHQSGSSLSHRPEPADVLVTEAGRS
                     VFRFETFGNELFFSDTLGLDDGWSRLGITPNSLLEAGVQFDGDKLPADFRRVDVGGGP
                     YTDPLATRRLIDANAVIGLVARQNRIGVTCAFCHSRADDRIGAGVGRRMDGAPNTALA
                     VGEALSWASRSRAYLPFLNVSGAGSGPLVSLGVTEDAGPAEAAVDAALRAWPRGQADI
                     LPDGVGNPTEIPSLFAVQAHGPYLWDGSFAKATDASQYFATIVFDPTTLATRWGRHYL
                     ADGPFWPVGEPLSASYQGVLRAIDPAASWPRATIFRASVFNLFHDALDAGFRVDRDDI
                     FAVTAYLRNLLPPDPEPADAHRIDAGRGVFRKAGCGACHLEGSVPGGAVVPLAVLVPG
                     YHAPVATTDDPVLAYDDRIARTPGEDSIDRRGYKVPSLLGLWLSAPYLHDGSVATLDA
                     LFDPARGDGSPHPYVVDAARDRRDLAAFLNAWDGRRYP"
     gene            complement(4233..5243)
                     /gene="fbp"
                     /locus_tag="AC16BC_07125"
     CDS             complement(4233..5243)
                     /gene="fbp"
                     /locus_tag="AC16BC_07125"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR3241429.1"
                     /translation="MRLTTIDQHIIAQERKFPGVSGEFSGLLYDIALAAKLVSKEVNR
                     AGLGDLLGSLGEVNVQGEIVQKMDKFANELFIRILSGGGRVCAIATEENERIVEIHDG
                     GFAGKYAINMDPLDGSSNIDVNVSVGTIFSLHRKRSPGVHGAEEDCLQVGSRQVGAGY
                     VIYGSSTMLVYTTGLGVHGFTLDPTLGEFILSHPDIKMPKRCTIYSVNEGNAAYWDAG
                     TRAYVDSLKVVDAGSGRPYSSRYIGSLVSDVHRNLLKGGIFLYPADHKDPKKPSGKLR
                     LLFEAAPMAFIAEQAGGAATTGTTRILDIEPTHLHQRVPLIIGPAEDVARYEDMVRRA
                     RG"
     gene            complement(5256..>5713)
                     /locus_tag="AC16BC_07130"
     CDS             complement(5256..>5713)
                     /locus_tag="AC16BC_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3241430.1"
                     /translation="LRRSLQGASQVAKRHGLDVRSLAEFREKHFGAWEGLTYDEVAQR
                     YPEDWARWLADPPSVRPEGGETYVEMQARVLDALGGVIAAHRAEAIALVAHGGVNRAI
                     LCHALGLDLTYVFRIEQDYGATNIIDFFPEGPAVVKAMNLLYWDQSADG"
BASE COUNT         1036 a         1975 c         1811 g          891 t
ORIGIN      
        1 atgacgcaat acaagcgcgc cgacctggtc ttctccgacc tgaagcgact cgcgcagatc
       61 ggcgggggcc ggctccagat cgtcctggcc ggcaaggcgc atccccagga cgcgcaaggc
      121 caacgcatcc tcgaacggct gttcaaggcc atggaggcgt tgcggaccga cgtgcccgtg
      181 gtcttccttc cggactacaa catggacatg gcgctcacga tcgtgagcgg gtgcgacctc
      241 tggctgaaca cgccgtgccg tccgctggaa gcctcgggca ccagcgggat gaaggccacc
      301 cacaacgggg tcccgaattt cagcgtgctc gacggctggt ggatcgaggg ccacgtggag
      361 ggcgtcaccg gctggtcgat cgggccgccg ccccgcgagt ccgctcccgc cgacgtggac
      421 gaccggcgcg acgcgcagga cctgtacgac aaactcgagc gcgtgattct cccgctgtac
      481 acgcaggacc gacagggctg gatcgcggtc atgaagcacg cgatcggcaa aaacgcctcg
      541 tattttaaca gtcaccgcat gatgcgccgg tacgtcaccg acgcctacct gcggtgacga
      601 cctagccacc agtatgaacc aggaaaggac catttccatg aacgcaccct ccccgttcga
      661 ttggctcaag gacctgtgca cccacttcga gtggcttcac ggcagcgcgc aagcgctggc
      721 cgacggccgt ccgatcgctc cgagcagcac gattccggat cacgcggtcg gcgaaatcat
      781 gcgcgcgttc ttccggatcc aggacacgat ggtcgcgcaa cagaccgagc gggacgcgtt
      841 gaagaccgcg gtcgagtcgc gcgacgcgga gctcgcgtcg ctccacgcgg aagccgacgc
      901 cgttcggcgc gagttggtcg aaacccgggc cgcgttgggc gagacccggc gaacgctcga
      961 agaccgccag cgcgggtggg aggaggccga ggccaaatgg cagcacgaac gacgcgtgat
     1021 ggacgaacgc ctcgcaggac ttgaacagcg cgttcgcgac gtgaccgggc ggctgactga
     1081 gcacgcccgc acgctcaacg cggtggcggc cgatctcgcc tcctcggcgt cgagctgatc
     1141 ggggcgccgc gattgcgcgg cgccggccgt tgtggtacaa gatccgggtt ttgccgtcac
     1201 acgaaggagt gtctatgcga atggcacggc tacgccgact gatgccgagc gcgtcgtcga
     1261 cccacttgag cagactggcg aacaaggtcc gacgcttcgt gctgtgtctg gttgcttccg
     1321 actatgtgca ggagcaggcc acgctccgcc aaggcgagtg cgtcggctgc gggcgatgct
     1381 gcaaactggt gtttcagtgc ccgttcctgg gagggaccga cgagaatccg cgctgtttga
     1441 tctataacgt ggcgcggccg aaccagtgcg ccgcgtttcc gatcgacgaa cgcgatctgg
     1501 ccgacgtgaa ttatctgtgc agttacagct tcgcgcagcc cgcgccgcag ccccaacaac
     1561 tgctcagcct cgtctacgaa tgagccgggc acgacctacc gcctgaatca cggacgacac
     1621 ggggacaccg ccatgcagga ccgcatccga acgattctca atcactacag caacgagaac
     1681 gcgggcacca agaccaacct cgcccgcctg ctcaatcacg gcgcgttggc cggcaccgga
     1741 aaattggtga tcctcccggt tgaccagggt tttgagcacg gcccggcgag aagtttcgcg
     1801 cccaacccct ccggatacga ccccgcgtac cacttccggc tggggatcga cgccggttgc
     1861 aacgcctacg ccgcgccgtt ggggtttttg gaagccgggg ccgcggaatt cgcgggcgac
     1921 atccccctca ttttaaagct caacaaccac gattcgctcc acgacgagaa agacccgctg
     1981 ccctcgcaaa ccggctcggt gcgggacgcg cttcgactgg gctgcgtggc ggtgggctac
     2041 accatctatc ccggttcgtc gcgctgccag accatgtacg aacagttgcg ggccatcgcg
     2101 gaagaggcca aggccgtcgg tctggccgtg gtggtgtggt cgtaccctcg gggatccggg
     2161 ctgtccaaag agggcgaaac cgcgatcgac gtggtcgcgt acgcggcgca gatcgccgcg
     2221 cagctcggcg cccatatcat caaggtcaaa cttccgacgg aacacgtcga acaggccgcg
     2281 gccaagaagg tctacgagtc caaccgcatc ccgatcacca ccctgaccga tcgggtccgc
     2341 cacgtggtgc aatccgcgtt caacgggcgt cggatcgtga ttttctccgg cggcgccaaa
     2401 gaggacgatg agcgcgtgtt caccgaagtg cgcgccatcc gggacggcgg gggattcgga
     2461 tcgatcatcg gccgcaactc cttccaacgc gcccactccg acgcgctgaa gttcctcgac
     2521 acgatcatga aaatttacgg cgggaagatc tcgtaaccga tcgccgcgat tccgactcgg
     2581 cggaggggac gccgcccggc tcccctccgc gaccgtacca cgttcgcgtc gaccgtacca
     2641 cgatcctcgt gtccccgccg ggcaacgacc tgcggtcgtt gcccgacacg ggctcagcaa
     2701 gcggtcatgg gtagcgtcga ccgtcccagg cgttgagaaa cgcggccaaa tcgcggcgat
     2761 cccgagccgc gtcgacaacg tacggatgcg gcgagccgtc gccgcgcgcc gggtcgaata
     2821 acgcgtcgag cgtcgccacg gagccgtcgt gcagatacgg cgcggacaac cacagcccca
     2881 ataacgacgg gaccttgtag cctcggcggt caatcgagtc ctcgcccggc gtgcgcgcga
     2941 tccggtcgtc gtaggcgagg accggatcgt cggtcgtcgc caccggcgcg tgatagccgg
     3001 ggaccagcac cgccaacggc accaccgcgc ctcccgggac cgagccctcc agatgacacg
     3061 cgccgcaacc cgccttgcgg aagacgcctc gcccggcgtc gatccgatgc gcatcggccg
     3121 gctcgggatc agggggcaac agattgcgca ggtacgcggt caccgcgaag atatcgtccc
     3181 gatcgacccg aaatcccgcg tccagcgcgt cgtgaaacag gttgaacacg ctcgcgcgaa
     3241 agatcgtggc ccggggccac gacgcggcgg ggtcgatcgc ccggaggacg ccctggtatg
     3301 aggcgctcag cggttctccg accggccaga acggaccatc ggccaaatag tggcggcccc
     3361 accgggtcgc caacgtggtg ggatcgaaga cgatggtcgc aaaatactgg gacgcgtccg
     3421 ttgccttggc gaacgaaccg tcccacaggt acggtccgtg cgcctgcacg gcgaacagcg
     3481 acgggatctc cgtggggttc cccaccccgt cgggcaggat gtcggcctgg ccccgcggcc
     3541 acgcccgcag cgccgcgtcc accgcggcct cggccggccc cgcgtcctcg gtcacgccca
     3601 gggacacgag cggccccgac cccgcgcccg acacgttgag aaacggcagg tacgcgcggg
     3661 aacgcgaggc ccacgacagc gcctcgccga ccgcgagcgc ggtgttcggc gcgccgtcca
     3721 tccggcggcc gacccccgcc ccgatccgat catccgcccg cgaatgacag aacgcgcagg
     3781 tcacgccgat tcgattctgg cgggccacca acccgatcac ggcgttggcg tcgatcaggc
     3841 gccgcgtcgc gagcgggtcg gtgtacgggc cgccccccac atccacccga cggaagtccg
     3901 ccggaagttt atccccgtcg aactggaccc ccgcctcgag cagggagttc ggcgtgattc
     3961 ccagtcgact ccatccgtcg tccaggccaa gcgtgtcgga gaaaaacagc tcgttgccga
     4021 aggtttcgaa ccgaaaaacc gaccgaccgg cctccgtcac caggacatcg gccggctcag
     4081 ggcgatggga taacgacgac ccgctctggt gggtgcaggc gatgagcgcc gacgcgccga
     4141 gccagacgac ccgacacaac cgtccgttca ccgcccggca ctcgcaggaa ggtctgaccg
     4201 gacggacatg gcggtcggtg cgcggtgagc cgtcaaccgc gcgcgcgcct gaccatgtcc
     4261 tcgtaccgcg ccacgtcctc ggccgggccg atgatcagcg ggacccgttg gtgcaggtgc
     4321 gtgggttcga tatcgaggat acgggtggtg ccggtggtgg cggctccgcc ggcctgctcc
     4381 gcgataaacg ccatgggcgc ggcttcgaac aacagccgca gcttgccgct gggtttcttc
     4441 gggtccttgt ggtcggccgg atacagaaaa atcccgccct tgagcaggtt gcgatgcaca
     4501 tcagacacca gcgacccgat gtaccgcgag ctgtagggac ggcccgatcc cgcgtcaacc
     4561 accttgagcg agtccacgta cgcgcgcgtc cccgcgtccc aataggcggc gtttccctcg
     4621 ttcacgctgt agatcgtgca tcgcttgggc atcttgatgt cggggtgcga caggatgaac
     4681 tccccgagcg tgggatcgag cgtgaacccg tggactccca gccccgtggt gtacacgagc
     4741 atggtcgagg acccgtagat cacgtacccc gcgccgacct gcctggatcc gacctgcaga
     4801 caatcctcct ccgcgccgtg cacgccgggg ctgcgtttgc ggtgcagcga aaagatcgtg
     4861 ccgacgctca cgttgacgtc gatgttcgac gaaccgtcca gcggatccat gttgatcgcg
     4921 tatttgccgg cgaagccgcc gtcgtgaatc tcgacgatcc gctcgttctc ctcggtggcg
     4981 atggcgcaga cgcggccccc gccgctcaga atccggatga acagctcgtt cgcgaatttg
     5041 tccatctttt gcacgatctc gccctgcacg ttcacctcgc ccagtgaccc gagcagatcg
     5101 cccaggccgg cgcgattgac ctccttggac accagtttgg ccgccaaggc gatgtcgtag
     5161 agcaacccgg aaaactcgcc cgacacgccg gggaacttcc gttcttgcgc gataatatgt
     5221 tggtcgatcg tggtcaaacg catcgcgact ccgtcctatc cgtcggccga ctggtcccag
     5281 tacagcaggt tcatcgcttt gaccaccgcc gggccctcgg ggaaaaaatc gatgatattg
     5341 gtggccccat agtcctgctc gattcgaaac acgtacgtca aatcaaggcc cagggcgtgg
     5401 cacaagatcg cccggttcac tccgccatgc gccaccaacg cgatcgcctc ggcccggtgc
     5461 gccgcgatca ctccgcccag cgcgtcgagc acccgcgctt gcatctctac gtaagtttct
     5521 cccccctccg gtcgcacgct gggaggatcc gccagccatc gcgcccaatc ctcgggatag
     5581 cgctgcgcca cctcgtcgta cgtgagaccc tcccatgcgc cgaagtgctt ctcgcgaaac
     5641 tccgcgagcg accgcacgtc caggccgtgt cgtttggcca cctgggaggc gccctggagg
     5701 cttcgccgca acg
//
LOCUS       DBNGTT010000316         4482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1091458, whole genome shotgun sequence.
ACCESSION   DBNGTT010000316 DBNGTT010000000
VERSION     DBNGTT010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1091458"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="AC16BC_07135"
     CDS             complement(<1..1028)
                     /locus_tag="AC16BC_07135"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011613558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate
                     methylesterase/protein-glutamine glutaminase"
                     /protein_id="HIR3241431.1"
                     /translation="MLVVDDSVVIRRLVSMALADCPNIEVVATAPNGRIALSKIPMYN
                     PDLITLDIEMPVMNGLETLAMVRKNHPSLPVIMFSALTPTDADKTLDCLSLGAQDYVT
                     KSTMGSGEAAVAYLRRHLIPKIELFCRGIAGASAPTASASEPAGPTAPAPKLFPEAVQ
                     RVDVLVIAASTGGPNALLEIVPQFPEDFPVPILIVQHMPRVFTEHFAKNLSEKSKIRV
                     EEGRSGQSLESGRAWVAPGDYHMALKREGALRCIQTNQNPPENSCRPSADVLFRSAAE
                     QYGPNTLAVVLTGMGQDGLRGSECIAAVGGQIVVQDEASSVVWGMPGFVAKAGLADAV
                     VPLNRIVA"
     gene            complement(1047..1421)
                     /locus_tag="AC16BC_07140"
     CDS             complement(1047..1421)
                     /locus_tag="AC16BC_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3241432.1"
                     /translation="MRALVIDDSKAMRAWMTSLLNEMGFEVFEACHGLQALERLTKNG
                     KVDLMLLDINMPEMDGFKFLRLVRSQHLQDSALILMVTSENGLDKVDAALKAGANEYI
                     MKPFSKEALHEKLRLAGILTAQ"
     gene            complement(1455..1781)
                     /locus_tag="AC16BC_07145"
     CDS             complement(1455..1781)
                     /locus_tag="AC16BC_07145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3241433.1"
                     /translation="MITVGMNYEILEGKEQPFERKFALVLEAMSQTPGHSNTNLYRDV
                     FRGRSYLIVSEWETRAAFNAFVQSDTFRSVTNWGRETILASRPRHEVYGDDVAAPLTA
                     GCPMHT"
     gene            complement(1852..3507)
                     /locus_tag="AC16BC_07150"
     CDS             complement(1852..3507)
                     /locus_tag="AC16BC_07150"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR3241434.1"
                     /translation="MELRQTEGWSVSILGRLLAPFPWWDDWSWNSPGDAATGRSLARW
                     WQLGRLAAGMARVRCRRPDRVAVRARQRVAERMGALHGLPQKIGQILSLSEIGRSDQI
                     YTPLTESRPALSASEAFSEMEQRLGRRLTDCFRSIEGEGISASLGQVHRAILPDGRWV
                     AVKVQYPAIRQALTTDLRALGWLTSPLGGLRRGFNLAAYQEEVGRMLTMELDYRREAR
                     MITRFAALAEGWTEIEIPKVIDGLSGDRILTMSWVKGEPIAAVRGWPLEARRQTATCL
                     ARLFLVSCFRWRLLHADPHPGNYRFLRRDGSIAVGLLDFGCVKPLDTATVDALAGLIH
                     DVIDLTRDEAPHRQDRLFARFLELGFQEELLAPLADRLVPLCRLVFEPFLSSGPYALD
                     RWDLGERAAEILGPYRWNFRFAGPPSLIFFMRAFQGLIQYVRALDAPVNWRDALEEAW
                     QGGAPPPAPVRMPHHEGNASMKSERLRVRIVDAGATLVDLTFRARAAEHLPDLLPEEL
                     RSTLDQRAIDVAGIAGAAVAREFQPGDLFELEEGERRIRVWLE"
     gene            complement(3515..>4482)
                     /locus_tag="AC16BC_07155"
     CDS             complement(3515..>4482)
                     /locus_tag="AC16BC_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR3241435.1"
                     /translation="KILANDKNLPVRAKIQIGPETADLLVSAIYDQNKNYLGPMVTWE
                     LVTEKVKEIMQVFDGVRKASDVLANSSQELTATNTQMGAAAQESTAQANTVAASSEQV
                     SKNVQNVATMVEEMNSSMKEISQNAEQATKVANSGVKMAESTTVTVGKLGESSNEIGN
                     VIKVITSIAQQTNLLALNATIEAARAGDAGKGFAVVANEVKELAKETAKATEDISRKI
                     EAIQGDAKGAVNAINQISDMIKKINEIQSTIASAIEEQTVTTNEIARNLGEASKGTSE
                     IAQNIGGVATATKQSADSIRGAQQSADSLARLAAELQTLVKSVKQ"
BASE COUNT          806 a         1520 c         1281 g          875 t
ORIGIN      
        1 tcggccacga tgcgattgag cggtaccacc gcgtcggcca ggccggcttt cgccacaaag
       61 ccgggcatcc cccaaaccac gctgctggcc tcatcctgca ccacgatctg cccccccacg
      121 gccgcgatgc attcggaacc gcgcagcccg tcctgcccca tgcccgtcag cacgacggcc
      181 agcgtgttcg ggccatactg ctcggccgcc gaacggaaca gcacgtcggc cgacggccga
      241 caggagtttt ccggagggtt ctggttggtc tgaatgcatc gaagcgcccc ctcccgcttc
      301 agcgccatgt ggtagtcgcc gggcgccacc cacgccctgc cggactcgag cgactggccc
      361 gacctgccct cctcgacgcg gatcttcgat ttctcggaca aatttttcgc aaaatgctcc
      421 gtgaagaccc gcggcatgtg ctggacgatc agaatgggca cggggaaatc ctccggaaat
      481 tgcggcacga tctccaacaa ggcgttcggc ccccccgtcg aggcggcgat caccagcacg
      541 tcaacccgct ggacagcctc ggggaacagt ttgggtgccg gcgcggtcgg ccctgccggc
      601 tcggaagccg acgccgtcgg cgccgacgcc cctgcgatcc cgcgacaaaa cagctcgatc
      661 ttcgggataa ggtgccgacg caaataggcg actgcggcct cgccggagcc catcgtgctc
      721 tttgtcacat agtcctgcgc gcccagcgag aggcagtcca gggttttgtc ggcgtccgtc
      781 ggcgtcagcg cgctgaacat gatgacggga agcgatgggt ggttcttccg aaccatcgcc
      841 agcgtctcca agccgttcat gacgggcatc tcgatgtcca gcgtgatgag gtccgggttg
      901 tacatgggaa tcttggagag ggcgatccgg ccgttcggcg cggtcgccac gacctcgatg
      961 ttcgggcaat cggccagcgc catggaaacc agccgccgga tcaccaccga atcgtccacc
     1021 acgagcacgc ggatttttcg catgggctat tgcgccgtca aaatcccggc cagcctcaac
     1081 ttctcgtgga gtgcttcttt ggaaaagggc ttcatgatat attcattcgc ccccgctttg
     1141 agcgccgcgt caactttatc gagaccgttt tcactcgtga ccatgaggat cagcgcgcta
     1201 tcctgcaagt gctgggaacg gaccagccgg agaaatttga agccgtccat ttcgggcatg
     1261 ttgatgtcca gcaacatcag atcaaccttg ccatttttgg tcaggcgctc cagcgcctgg
     1321 agcccatggc acgcctcaaa tacctcaaac cccatctcgt tcagcagact cgtcatccag
     1381 gctcgcatgg cttttgaatc atcgatcacc agtgcccgca tgtgtgcctc ccgccgctag
     1441 gtgacgcgcc gccttcaggt gtgcatcggg cagccggccg tcagcggggc ggccacgtcg
     1501 tctccgtaga cctcatgcct ggggcggctg gccagaatgg tctccctccc ccaattcgtc
     1561 acactgcgga aggtatcgga ctggacaaaa gcattgaagg cggcccgcgt ctcccattcc
     1621 gagacgatga ggtaggagcg gccgcggaag acatcgcgat agagattcgt gttgctgtgt
     1681 cccggcgttt ggctcatcgc ctccagcacc agcgcgaatt ttctctcgaa cggctgctcc
     1741 ttcccttcga gaatctcata gttcatgccc acggtgatca tcagtcgcct ccctggttgt
     1801 ttgttgactt gccgtccttc acgcgtcgtg atcgtgcatc acccgaacga atcattcgag
     1861 ccagacgcga atgcgccgct ccccctcctc cagctcgaac agatcgccgg gttgaaactc
     1921 ccgcgccacc gccgcccccg caatacccgc cacgtcaatg gcgcgctggt ccagcgtcga
     1981 tcgcagttcc tcggggagca ggtccggcaa gtgctcggcc gcccgcgccc gaaaggtcag
     2041 atccacgagc gtagcgcccg cgtcgacaat tcggacgcgc aatctctccg acttcatcga
     2101 tgcattcccc tcgtggtgtg gcatccggac cggcgccggg ggtggcgcgc ccccctgcca
     2161 tgcctcttcg agcgcgtcgc gccagttgac cggcgcgtcc agcgccctca catactgaat
     2221 caacccctgg aaggcgcgca tgaaaaaaat caggctgggc ggccccgcaa accggaagtt
     2281 ccatcgatag gggcccagaa tctcggcggc gcgctcgcca agatcccatc gatccagcgc
     2341 ataggggccc gacgacagaa agggttcaaa caccaggcgg cagagcggta ccagccggtc
     2401 ggccaacggc gccaacaact cctcctggaa acccaactcc agaaaccgcg cgaagagccg
     2461 atcctgccga tgcggcgcct cgtcccgcgt caggtcgata acatcgtgga tcaacccggc
     2521 caaggcatcg accgtcgcgg tatcaagtgg cttgacgcaa ccgaaatcga gtaaccccac
     2581 cgcgatcgag ccgtccctgc ggaggaaccg gtagttgccg gggtggggat cggcatgcag
     2641 aaggcgccac cggaagcaac tcacgaggaa gaggcgcgcc aggcaagtcg ccgtttgccg
     2701 acgtgcttcg agcggccatc cacgcaccgc cgcaatcggc tcgcccttca cccagctcat
     2761 ggtgaggatg cggtcgccgc tgagtccgtc gatcaccttc ggaatctcga tctccgtcca
     2821 cccctcggcc aatgcggcga agcgcgtgat catccgcgct tcgcggcggt agtcgagctc
     2881 catggtaagc atgcgaccga cttcctcctg gtaggcggcg agattgaacc cgcgcctcag
     2941 gccgcccaac ggggaggtca gccaccccag cgctcgcagg tcggtcgtga gcgcctgtcg
     3001 aatggccgga tactggacct tcaccgcgac ccaacgtcca tccggcaaga tcgctcgatg
     3061 aacctgcccg agcgaggccg aaatgccctc tccctcaatg gagcgaaagc agtccgtcag
     3121 ccgcctcccg aggcgttgtt ccatctccga aaaggcctct gatgcggaca atgccggccg
     3181 agattcagtc aacggcgtat agatctgatc gctccggccg atctcggata gcgaaagaat
     3241 ctgcccgatt ttttgcggca ggccatgcaa cgcgcccatc cgctcggcca cacgctgacg
     3301 ggcccgcact gcaacccggt cgggacgccg acagcggacc cgggccatcc ccgcggccag
     3361 ccgtcccaat tgccaccacc gcgcaagcga tcggcccgtg gccgcatcgc ccggtgagtt
     3421 ccacgaccag tcgtcccacc agggaaacgg cgcgagcagg cggcccagga tggagacgga
     3481 ccagccctct gtctgccgga gttccacctc cggtctattg tttgacgctc ttaacaaggg
     3541 tctgcagctc cgccgccagt cgcgccaggg aatccgccga ttgttgcgcc ccacggatgc
     3601 tgtccgcgga ctgcttcgtg gcagtggcca cccccccgat gttctgggca atttcactcg
     3661 tgcccttcga cgcttcgccg aggttgcggg cgatttcatt ggtggtcacg gtttgttcct
     3721 cgatggcgct ggcgatggtg ctctgaatct cattgatctt tttgatcatg tcactgatct
     3781 ggttaatcgc gttgaccgct cctttcgcat cgccttgaat cgcctcaatc tttcgactga
     3841 tgtcctcggt ggcctttgcg gtttccttgg ccagttcctt cacttcattg gcaaccaccg
     3901 cgaaaccttt cccggcatcg cccgcgcgcg cggcctcaat ggtcgcgtta agggccagca
     3961 ggttggtctg ctgcgcgatg gaggtaatga ccttgatgac attcccgatc tcgttgctgc
     4021 tctcaccaag ctttccaacc gtgacggtcg ttgactcggc catcttgacc ccggaattgg
     4081 cgaccttcgt cgcttgctcc gcgttttggg agatttcctt catgctgctg ttcatctctt
     4141 ccaccatcgt ggccacgttt tgaacgttct tgctcacctg ttcggacgag gcggcgaccg
     4201 tattggcttg agcagtggac tcctgggccg cggcccccat ctgggtgttg gttgcagtta
     4261 actcttgcga ggaattggcc aacacatccg acgccttcct cacaccatca aatacctgca
     4321 tgatctcttt gaccttctcg gtgacaagct cccaggtcac catgggcccc aggtagttct
     4381 tgttctgatc atagatcgcg ctcaccagca gatcggcggt ttccggccca atctgaatct
     4441 tggcccggac cggcaggttc ttgtcgttgg ccagaatctt gc
//
LOCUS       DBNGTT010000317         5453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3391706, whole genome shotgun sequence.
ACCESSION   DBNGTT010000317 DBNGTT010000000
VERSION     DBNGTT010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3391706"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="AC16BC_07160"
     CDS             complement(<1..1502)
                     /locus_tag="AC16BC_07160"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR3241436.1"
                     /translation="MTPRWVAAYEPGISPTLQYPVVPLHHLLSASAARYPERPALDFY
                     GRRLTYQDVERAANRFADALTGLGVRKGDRVAVMLPNVPQAVIAYYGALKIGAVVVQV
                     NPLYVEREIEQQVRDAEARVLVALDLFYPRIEAVRSRAGLEAVIVTSVRDALPWYLGL
                     LYPLKTWREGARVHVPKAPPVYNFESLLAGAREDTPRVDVGPDDLALLQYTGGTTGVP
                     KGVMLTHRNLVANTLQCRAWMPSLRDGEEVFLGVIPFFHVYGMTACMNLAIATGGLIV
                     LLPRFVTKDVLRAIVKSRATIFMGVQAMYVAINNFPDVRRYDLSSIRICISGAGPLHV
                     EVQERFEALTGGKVVEGFGLSEASPVTHVNPIFGRRKKGTIGLPLPDTEAKVVDVESG
                     TREMPLGEAGELLIRGPQIMAGYWRRPDETAAVLRNGWLHTGDVASIDSDGYFTIVDR
                     KKDMIKTKGENVYPRDVEEALFRHPKIKDAVVVGVPEEFMGEMIKAYVVLR"
     gene            1797..2399
                     /locus_tag="AC16BC_07165"
     CDS             1797..2399
                     /locus_tag="AC16BC_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3241437.1"
                     /translation="MAPTLDKGTFLIAGPGMYDPNFRQSVVLLCEHNDRGSMGLVINR
                     PTELVLTEAVHQLAEADRDDLIFSGGPVQPDHLLILIRSESAPAASHHVFGDVYLGTD
                     MPTLKSLLHPDADRESPTDTAEAAAKPSEAAFRGYAGYAGWAPGQLDAEMATDSWIVV
                     PADSKWIFDADPKVVWPELMRSLGPRHAKYATMPRDPSLN"
     gene            complement(2407..5385)
                     /locus_tag="AC16BC_07170"
     CDS             complement(2407..5385)
                     /locus_tag="AC16BC_07170"
                     /inference="COORDINATES: protein motif:HMM:NF018269.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3241438.1"
                     /translation="MSEHPRHGRQERVAGPRVGWSGARAVVGAAVLASLAAASVADAQ
                     LSERPGTSEDTALGAKDVTGRSVKDARVRIVHSSDPAQEGTSRYLQDVDPWLAYQRGR
                     NLTQREFSARDGVFGRAGSFFEPKLLGDRVTPRIGRDHVNSCGICHAIPFREPGAGGN
                     IPKGSGEGRNATHFFGGGLIEMIGLQTRLKVLQACDPSRRGFVPTHGVPDAPIRIAPT
                     PGGSPVDYGWCGDRNNDGAPDLNKIFRVWYVDEAGRRVTEDGNGDGVINLRDPNVAGY
                     NLEMMVFGWGETEGAMAPTLRVFFTDPIDTHSGMQAYDPTIQVDDGRHGDGGAGDGLA
                     AVSNAGARQFAIHAPADRGLRVSDRGVSLDDPDGDGVVNEISEGDVDVAEWYMLNAPA
                     PGVGPQTSQVRRGRAVFQAMGCATCHIPDWLLETASPEAADPYRRYDGDRRFFDLSVE
                     YRERSGRLEGALTLLADHRNGRWAPRRDAALVKGVFSDFKHHDMGPAFAETLFNGARL
                     TRFRTAPLWGVGSSAPYGHDGTSLTLDEVIRRHGGEAENAGARYRGASAGDRAAVVAF
                     LQSLVLYQSDRLPTDLDADGAVSPHFMVAGRDTGEERFNPEWLFRRPCLIEGPVRAPD
                     GSATTSFACVNVSDAYGESLAWLSDSDADGFPDVIDPCPHDEDGGRACFLDEGREAGS
                     GSGSEVVTVVPTTVSLAPDPREPRVVWAAGTDGWGIRRSLDGGRTWRVMTNEPRLLHA
                     TAVAVNPGRSEVVYAGSPYGVFRSVDRGATWDALTNGMSDTRIFSVAVNPRSPWTVYA
                     GGFGGRVYRSTDGGTTWIETRLGTDVYRVSALAVDAGESSVPALDNRLPALYAATSGG
                     GVFRSDDGGAGWSALPGLPDGTVYTVALDSRVPTTLYVGTPTGVYRSLDRGARWAPAG
                     LGAETPALSLAVDPQVPGCVVAGTPDGVVMTADGGRNWRRLSGVAADRPVVSVVLDPW
                     NPRSVYAAMFGGGVVEGTAR"
BASE COUNT          927 a         2013 c         1638 g          875 t
ORIGIN      
        1 cgcagcacca cgtacgcctt gatcatctcg cccatgaact cttcgggcac cccgaccacc
       61 acggcgtctt tgatcttcgg atggcggaac aaggcctcct cgacgtcacg ggggtacacg
      121 ttctcccctt tggtcttgat catgtccttt ttgcggtcca cgatcgtaaa gtacccgtcg
      181 gagtcgatgc tggccacgtc acccgtgtgc aaccacccgt ttcgaagcac cgccgcggtt
      241 tcatcgggcc ggcgccagta ccccgccatg atctgggggc cgcggatcag caattccccg
      301 gcctcgccca gaggcatctc ccgcgtgcca gactccacgt ccaccacttt agcctcggtg
      361 tcgggcaacg gcaacccgat cgtccccttt tttcgccgcc cgaagatggg gttcacgtgc
      421 gtcacgggag acgcctcgct cagcccgaac ccttcgacca ctttgccgcc ggtcagcgcc
      481 tcaaaccgct cctggacttc cacgtggagc gggccggcgc cgctgataca gattcggatc
      541 gacgacagat cgtatcgccg cacgtccggg aaattgttga tcgcgacgta catcgcctgc
      601 acgcccatga agatcgtggc gcgcgacttc acgatggcgc gcaacacgtc tttggtcacg
      661 aaccgcggga gcaggacgat caacccgccg gtcgcgatcg cgaggttcat gcaggcggtc
      721 attccgtaca cgtggaaaaa cggaatcacg cccaagaaca cttcttcccc gtcccgcagc
      781 gacggcatcc acgcccggca ctgcaaggta ttggcgacga ggttccggtg ggtcagcatc
      841 acgcccttgg gaacgccggt cgtgcccccg gtgtactgta agagcgccag atcgtctggc
      901 cccacgtcga cccggggcgt gtcctcccgc gctcccgcga gcagcgactc aaaattatag
      961 accggaggcg ccttgggaac gtgcacccgg gcgccttccc gccaggtctt gagcgggtag
     1021 agcagcccca ggtaccacgg caacgcgtcc cggaccgacg tgacgatcac cgcttccaac
     1081 cccgcgcggc ttcggaccgc ctcgattctg gggtagaaga ggtccagcgc caccaacacg
     1141 cgggcctccg cgtcccggac ttgttgttcg atctcccgct cgacgtagag cggattgacc
     1201 tgaaccacca cggcgccgat tttgagcgcg ccgtaatacg cgatgaccgc ctgaggcacg
     1261 ttgggcaaca tcaccgccac ccgatccccc ttgcgaacgc ccaagccggt caatgcatcc
     1321 gcaaaacggt tcgcggctcg ctccacatcc tgataggtaa gacgtcgacc gtagaagtcc
     1381 agcgcgggac gctccggata acgagccgcc gacgccgaga ggagatgatg aagggggaca
     1441 accgggtact ggagtgtcgg cgaaattccc ggttcgtacg cggccaccca acgcggggtc
     1501 acccggcggc tccacgtcgt cggcacgcgg cgtgcacacg gtgccggtgt atgccgaggc
     1561 tgcagttcac gtccttatgc gtcccaatca tccctttggg gtcttccccg acccgggatt
     1621 catcctgcag cggacggcgc tcctataata tataccggaa agatctacgt ctcgtcgccg
     1681 accggagctg actctagccc aggggtcagg ctttgtcaag aaaccacagc gccgtctcgc
     1741 gagcgcaggg agccctccgc tggactttcc gccggggcgg ttataggatg gaaaccgtgg
     1801 cccccacgct cgacaaaggc acattcctca tcgcgggtcc ggggatgtac gaccccaatt
     1861 tccgtcagtc ggtggtcctg ctctgcgaac acaacgaccg cgggagcatg ggcctcgtca
     1921 tcaaccgccc gaccgaactc gtgctgaccg aggccgttca tcaactggcg gaggcggatc
     1981 gggatgacct gatcttttcc ggaggcccgg tccagccgga tcacctgttg atcctcatcc
     2041 gctccgagtc ggctcccgcg gcgtcccatc acgtgttcgg cgacgtctac ctcggcaccg
     2101 acatgccgac cctcaagtcg ttgctccatc ccgacgccga ccgggagtct ccgaccgata
     2161 ccgccgaggc ggcggccaag ccgtcggagg cggcgtttcg aggctatgcc ggctacgcgg
     2221 gatgggcgcc cggccaactc gacgccgaaa tggccacgga ttcatggatc gtcgtgccgg
     2281 ccgactccaa atggatcttc gacgccgacc ccaaagtcgt ctggccggaa ctcatgcgct
     2341 cactgggtcc tcgacacgcc aagtacgcca cgatgccgcg ggatccgagc ttgaactgac
     2401 cggctcctac cgggcggttc cctcgaccac gccgccaccg aacatcgcgg cataaaccga
     2461 ccggggattc cacgggtcga gcaccacgga cacgaccgga cgatccgcag ccactccgga
     2521 taaacgccgc cagttccgcc cgccatccgc ggtcatcacc acgccgtcgg gcgtgccggc
     2581 gaccacgcag cccggcacct ggggatcgac ggcgagggac agcgccggcg tctcggcccc
     2641 cagaccggcc ggtgcccacc gcgcgccgcg gtccaggctc cggtacacgc ccgtcggcgt
     2701 gccgacatag agcgtggtgg gcacgcgcga gtcgagggcc acggtgtaca ccgtgccgtc
     2761 cggcaacccg ggcaacgccg accaccccgc ccccccgtcg tcgctgcgga aaaccccgcc
     2821 gccgctcgtc gccgcgtaca acgcgggcag tcggttgtcc aacgccggaa cgctcgactc
     2881 cccggcgtcc accgcgagcg cgctgacccg atacacatcg gtcccgagcc gtgtttcgat
     2941 ccacgtggtg cctccgtccg tggaacgata gacccgaccg ccgaatcccc ccgcgtagac
     3001 ggtccaggga gatcgcggat tcacggccac cgaaaagatc cgcgtatcgc tcatcccgtt
     3061 ggttagcgcg tcccaggtcg cgccgcgatc aacgcttcgg aataccccgt aaggagagcc
     3121 ggcgtacacc acttcggacc gaccgggatt gactgccacc gccgtggcgt gcagcagacg
     3181 aggctcgttc gtcatcaccc gccaggtccg ccccccgtcc agactccgcc ggatacccca
     3241 accatcggtt ccggccgccc acaccacgcg cggttccctg ggatctgggg ccaaagacac
     3301 ggtggtggga acgactgtga ccacctcgct tcccgatccc gagcctgcct cacgtccctc
     3361 gtcaaggaaa cacgcgcggc ccccgtcctc gtcgtgcgga cagggatcaa tcacgtccgg
     3421 aaatccatcc gcatcggaat cgctcagcca cgccagcgat tccccatagg cgtcggatac
     3481 gttgacgcag gcgaacgacg tggtcgccga cccgtcgggc gcgcgaaccg gcccctcgat
     3541 caggcacggc ctgcggaaca accactccgg attgaagcgc tcctccccgg tatcccgacc
     3601 ggcgaccatg aagtgcggcg acaccgcgcc gtcggcgtcg aggtccgtgg gcagacggtc
     3661 gctctgatac agcaccagac tctgcagaaa cgcgaccacc gcggcgcgat cccccgctga
     3721 cgcgccccgg tagcgcgcgc ccgcgttttc cgcttccccg ccgtgtcgcc ggatcacctc
     3781 gtccagcgtc aaactcgtgc cgtcgtgacc atagggcgcc gaactcccca cgccccacaa
     3841 cggcgcggta cgaaaccgcg tgagcctggc gccgttgaac aatgtctcgg cgaacgcggg
     3901 ccccatgtcg tggtgtttga agtccgagaa cacgcccttc accaatgcgg cgtcgcgcct
     3961 tggcgcccac cgcccgttcc ggtggtcggc caaaagcgtc agcgcccctt cgaggcgacc
     4021 cgaccgctcc ctgtactcga ccgacagatc gaagaaccgc cggtccccgt cgtaccgtcg
     4081 gtacggatca gcggcctccg ggctcgcggt ttcgagcagc cagtccggga tgtggcacgt
     4141 cgcacagccc attgcctgaa acacggcgcg tccccgccga acctgggacg tctgcgggcc
     4201 aaccccgggc gcgggcgcat tcagcatgta ccactcggcc acgtccacgt ccccttcgga
     4261 aatttcgttg accacgccgt cgccgtccgg gtcgtcgaga ctcaccccgc ggtcgctcac
     4321 gcggaggccg cggtcggccg gcgcgtggat ggcgaactga cgcgcgccgg cgttggacac
     4381 cgcggccaac ccgtcgccag cgcccccgtc gccgtgccgc ccgtcatcga cctgtatggt
     4441 cgggtcgtag gcctgcatgc cgctgtgggt gtcgatcggg tcggtgaaaa acacgcgcag
     4501 cgtgggggcc atcgcgccct cggtctcgcc ccacccgaac accatcatct cgaggttgta
     4561 ccccgccacg ttcgggtcgc gcaggttgat cacgccgtcg ccgttgccgt cctcggtcac
     4621 ccgtcggccc gcctcatcca cgtaccacac gcggaagatt ttgttgaggt cgggggcgcc
     4681 gtcgttgttg cggtccccgc accagccgta gtcgacgggc gatccgccgg gagtcggcgc
     4741 aatcctgatc ggcgcatccg gaaccccgtg agtgggcaca aacccgcggc gcgagggatc
     4801 gcacgcctgc aacaccttga gccgcgtctg caggccgatc atctcgatca acccgccccc
     4861 gaaaaagtgc gtcgcgtttc gtccctcgcc gcttcccttg gggatgttgc cgccggcgcc
     4921 gggctcccga aacggaatcg cgtggcagat cccgcacgag ttgacgtgat cgcgaccgat
     4981 gcgcggggtc acgcgatcgc cgagcagttt gggctcgaag aacgatccgg cgcgtccgaa
     5041 caccccgtcg cgagcgctga attcgcgttg cgtgaggttt ctccctcgct ggtaggccaa
     5101 ccagggatca acgtcctgca agtatcgcga cgtgccttcc tgcgcgggat cagaactatg
     5161 gacgattctc acgcgcgcgt ccttgaccga gcgacccgtc acgtctttcg cgcccagcgc
     5221 ggtgtcctcg gacgtacccg gccgctcgga caattgcgcg tccgcgacgg acgcggccgc
     5281 cagggacgcc agcaccgccg cccccacgac ggcacgcgcg ccgctccatc cgacccgtgg
     5341 tcccgcgacc ctctcctgac ggccgtgtcg tgggtgctca gacattcgtc cctccggtca
     5401 gcgcaacgtc tcctcgttcg ggaaccgatt gttccagccc ccaccaccgg ccg
//
LOCUS       DBNGTT010000318         4452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1220146, whole genome shotgun sequence.
ACCESSION   DBNGTT010000318 DBNGTT010000000
VERSION     DBNGTT010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1220146"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="AC16BC_07175"
     CDS             <1..253
                     /locus_tag="AC16BC_07175"
                     /inference="COORDINATES: protein motif:HMM:NF037182.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR3241439.1"
                     /translation="QVIDAAKRLARAAATESAAGVSVLGPAPAPLWRLKGRHRWQLVL
                     KGPQGPGVRRTARALMARAGTDLPRGVRVDVDVDPQHVL"
     gene            complement(248..1030)
                     /locus_tag="AC16BC_07180"
     CDS             complement(248..1030)
                     /locus_tag="AC16BC_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3241440.1"
                     /translation="MGSGARWSGCGGCAGSTRPASAPIAWRVGMPRSADRPVRPGRDC
                     ALVVFAKAPIPGTVKTRLVPPLTHPQAARLHAAFVEDTLQKVARLDMSRYLAYTPTPG
                     TPSLLACARRYHARMIAQGAGDLGARMQRVTQRLLSRYSKVLIIGTDSPTLPVAFIEE
                     ARRQLDRADLVFGPSEDGGYYLLGQRRLCPDIFTGIGWGGPDVLAATLKKVPRERVAL
                     LPTWYDIDRPSDLVRLRADLNGATDCPKTTAWFRAHERRTRL"
     gene            complement(927..1634)
                     /locus_tag="AC16BC_07185"
     CDS             complement(927..1634)
                     /locus_tag="AC16BC_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3241441.1"
                     /translation="MDERRRPLPFITVSIIVPTLDEEVGIREALTAAIAVTPHEIIVV
                     DGGSADRTRALAEACGCRVLDAPRSRAVQMNAGARAASGDLLLFLHADTRLPPDAIPQ
                     MAHAMADERAVGGRFDVSIDGSEPLFRVIETLMNLRSRVTRVATGDQAIFVRRRVFEA
                     LGGFAPIPLMEDVQFCARLKRRGRVVCLRARVRTSARRWRRHGIWRTMVRMWWLRGLY
                     AAGVSPDRLARWYAPVR"
     gene            complement(1681..1956)
                     /locus_tag="AC16BC_07190"
     CDS             complement(1681..1956)
                     /locus_tag="AC16BC_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241442.1"
                     /translation="MTDPLLRIERRASGIYHLLFHAGPYYWPVSDEMLTRLHAQSSGS
                     PDTFLAVLMDTVAVTRYLKHQLTEVLAGADDRDAQLRALQAALAVKK"
     gene            complement(1995..2474)
                     /locus_tag="AC16BC_07195"
     CDS             complement(1995..2474)
                     /locus_tag="AC16BC_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR3241443.1"
                     /translation="MAAGLYEGTLAEAIRRCKYHQQVELILVLAELLDREVKTLPPVD
                     AVVPVPLHVRRLRQREFNQSLRLGALLARRLTRPLWPDALRRTRWTAPQTTLDRAHRQ
                     ANVRRAFAVRQPQAVAGRRLLLVDDVYTTGATVNECAKTLAAAGAADVYVVTVARLP"
     gene            complement(2517..2720)
                     /locus_tag="AC16BC_07200"
                     /pseudo
     CDS             complement(2517..2720)
                     /locus_tag="AC16BC_07200"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
     gene            2818..3267
                     /locus_tag="AC16BC_07205"
     CDS             2818..3267
                     /locus_tag="AC16BC_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR3241444.1"
                     /translation="MSVRRILAALTACVLLSAARGAGAEGLSIDRGTVTLPNGAVYAV
                     EIPRTQEARTRGLMFRATLPRRTGMLFDFETTGRYGFWMKNCLVSLDLIWIDEAKRVV
                     AILPETPPCKADPCPIYEFDVPTRYVLEIPSGAAKREGLVLGSVLKF"
     gene            complement(3316..>4452)
                     /locus_tag="AC16BC_07210"
     CDS             complement(3316..>4452)
                     /locus_tag="AC16BC_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3241445.1"
                     /translation="SPARILLAVGLLSAGLSLWVSNTATAAMMLPIVSGMLGALAGVR
                     RDPAGNPASPAAWRYSAALMLMVAYGASIGGLGTVIGTPPNLIGKGLIEERLGVDIGF
                     VQWMQFGMPIVIVTFAALFALLYALHPPDLGSLTGVSEFLARQRARLGRMSRGERNAA
                     VAFGTAIVLWVAPAGLALVGGTDSAAATWYEAHVPEGVVALLAASLLFLLPVDWAARR
                     FTLSWEEAERIDWGTILLFGSGLALGDLMFKTGLSGAIGRGAMAWMGADSLWVITGAA
                     VAFGILLSELTSNTASATMVIPVVIAVAQGAGVSALPPALGACLGSSFGFMLPISTPP
                     NAIVYGSGLIGIGQMIRAGVLLDVIGFFVIWGGLRILCPLLGLT"
BASE COUNT          777 a         1481 c         1506 g          688 t
ORIGIN      
        1 ccaggtcatt gacgcggcga agcggctggc tcgcgccgcg gcaacggagt ccgccgccgg
       61 ggtttcagtg ctcggcccgg cgcctgctcc gttgtggcgg ctcaaggggc gccaccgctg
      121 gcagctcgtg ctcaagggcc ctcagggccc gggcgtccgg cggaccgcgc gcgcgctgat
      181 ggcgcgcgcc ggaaccgacc ttccccgggg ggtaagggtc gatgtggacg tggacccgca
      241 acacgtctta taagcgcgtg cggcgttcgt gcgcccgaaa ccacgccgtg gtcttcgggc
      301 aatcggttgc tccgttcaga tcggcgcgca ggcgcaccag gtcgcttggg cggtcgatgt
      361 cgtaccatgt cgggagcaac gcaacgcgct cacgcgggac tttcttgagc gtggcggcca
      421 agacgtccgg tcctccccag ccaatgcccg tgaaaatgtc cggacagagt cgtcgctggc
      481 ccaagaggta atatcctccg tcttcggaag ggccgaagac caggtcggct cgatccaact
      541 gtcggcgcgc ctcttcaatg aacgcgaccg gcagcgtggg actgtccgtg ccgatgatca
      601 ggactttgga gtagcgagag agcaagcgct gcgtgacccg ctgcatgcgg gcgccgagat
      661 ctccggcccc ttgggcgatc atccgcgcat ggtatcggcg ggcgcaggcg agcagggacg
      721 gggttcccgg tgtcggtgtg taggccagat aacgtgacat atcgagcctt gcgacttttt
      781 ggagcgtgtc ctccacgaat gcggcgtgta ggcgcgcggc ctgtggatgc gtcagcggtg
      841 ggacgagccg tgttttgacg gttcccggaa tgggagcctt ggcgaacacg accagggcgc
      901 aatcccgtcc ggggcgaacc ggacggtcag cggaccgggg cataccaacg cgccaagcga
      961 tcggggctga cgccggccgc gtagagcccg cgcagccacc acatccggac catcgtgcgc
     1021 cagatcccat ggcgccgcca ccggcgcgcg gacgttcgca cccgcgcacg gaggcatacg
     1081 acgcggcccc ggcgcttcaa gcgggcgcag aattgcacgt cttccatgag cgggatgggg
     1141 gcgaagccgc cgagcgcctc gaacacgcgt cgccggacaa agatggcctg atcgccggtg
     1201 gcaacgcgcg tgacgcggga acgaaggttc atcagcgtct cgatgacccg gaacagtggc
     1261 tccgagccat cgatggagac atcaaagcgg cctcccaccg ctcgttcgtc cgccatcgcg
     1321 tgggccatct gcggaatagc gtccggtgga agcctcgtgt cggcgtggag gaacaggagg
     1381 aggtctccgc tcgcggccct ggctccggca ttcatctgta cggcgcggct gcgcggcgca
     1441 tccagtacgc gacatccgca ggcctccgcc agggcgcgcg tgcgatccgc gctccccccg
     1501 tccaccacga tgatctcgtg cggcgtgacc gcgatggcgg cggtcagcgc ctctcgaata
     1561 ccgacctcct catcgagggt cggaacaatg atcgatacgg tgatgaaggg aagcggcctg
     1621 cggcgttcgt ccaacgaacg atcccggtcc cgcgggcccg tggacaccgc ggggcgcgac
     1681 ctattttttt acggccaggg cagcttggag ggcgcggagc tgtgcgtccc ggtcgtccgc
     1741 cccggccagg acctccgtca attggtgttt caagtagcgg gtgacggcga ccgtgtccat
     1801 caagacggcg agaaacgtgt ccggactgcc ggaactctga gcgtgaagcc gcgtcaacat
     1861 ctcgtccgag acgggccagt agtacggccc ggcgtgaaac agtaggtggt aaattccgga
     1921 cgcgcgtcgt tcgatacgaa gcagtggatc ggtcatgggt cgctgactcc tggtgaggtc
     1981 atggcacggc gaacttaggg aagacgcgcc acggtgacaa cgtacacgtc agccgcgccc
     2041 gcggcggcca acgtcttggc gcattcgttc acggtggctc cggtggtgta gacgtcgtcc
     2101 accagcagga gccgccggcc ggcaaccgct tgcggctgac gcaccgcgaa cgcgcgccgg
     2161 acattggcct ggcgatgcgc gcgatccagc gtggtttggg gcgcggtcca gcgcgtgcgg
     2221 cgcagcgcgt ccggccacaa cggtcgggtg agtcgacggg cgagcagcgc gccaagccgc
     2281 agcgactggt tgaattcccg ctgccggagc cgtctgacgt gtaacgggac cgggaccacc
     2341 gcgtcgacgg gcgggagcgt cttgacctcg cgatcgagca gttcggccaa caccaggatc
     2401 aactcgactt gttggtggta cttgcatcgg cggatcgcct cggccagcgt cccctcatag
     2461 agtccggcgg ccaccgctcg ggtaaacgcc ggcggggcct cccggcacga gccgcatcgg
     2521 tgcgtgggac tgtgagaaac cgccgcctcg gatggaaacg gcgtcccgca ccgcggacac
     2581 cgcggtccct tgatgcgggg tatggcgctc caacacgttg cgcagatcgc gcgggcctcg
     2641 tccgcgaaca gggctcggcg gcacaccgcg caccggcgcg gcaggatcgc atcgagtgcc
     2701 gaccgaacaa ggccccacat ccagcgacgc tttctaacac aacgtcctag gggtgtcaac
     2761 gcgttgccct gacactgtgt tgcggcgggt tcggcgttct ggtagcgtag ccggcgtatg
     2821 agcgtgcgac gtatcctggc ggcccttacc gcgtgcgtgt tgctcagcgc ggcgaggggt
     2881 gccggtgccg agggcctgtc gatcgaccgc gggaccgtca ccctgcccaa cggcgcggtg
     2941 tacgcggttg aaatcccgcg aactcaggag gccaggaccc gcgggctcat gtttcgggcc
     3001 acgctgccgc gtcgcaccgg catgttgttc gacttcgaga caacgggccg ctacggattc
     3061 tggatgaaga actgtctggt ctcgttggat ttgatctgga tcgatgaggc gaagcgcgtg
     3121 gtcgcgattc tccccgaaac cccgccgtgc aaggccgatc cgtgtcccat ctacgagttc
     3181 gacgtgccga cccgttatgt cctggaaatc ccgagcggag cggccaaacg tgagggactg
     3241 gtgctcggca gcgtcctcaa gttttaatcc acccacgtcc cgattgcgac ggtccgcgac
     3301 cgttgtcgcc gtgaattagg tgagccccag cagcgggcag aggattcgca acccgcccca
     3361 gatcacgaag aatccaatga cgtccagcag cacgccggcc cggatcattt gcccgatgcc
     3421 gatcaggccg gagccataca cgatggcgtt gggcggggtc gagatcggca gcatgaagcc
     3481 gaagctcgat cccaaacagg cccccaacgc cgggggcaac gcgctcaccc ccgcgccctg
     3541 cgcgaccgcg atgaccaccg ggatcaccat ggtggcggac gcggtgttgg aagtcagttc
     3601 gctcagcaga atgccgaagg ccacagccgc cccggtgatc acccacaggc tgtccgcgcc
     3661 catccacgcc atggcgccgc gtccgatcgc gcccgataac ccggtcttga acatcaagtc
     3721 gcccagcgca aggccgctac cgaacaacag gatcgtgccc cagtcgatgc gttccgcttc
     3781 ttcccacgac aaggtaaatc gccgcgcggc ccagtccacg ggcagcaaga acagcagcga
     3841 ggcggcgagc agcgccacca cgccttcggg gacgtgcgcc tcgtaccatg ttgcggcggc
     3901 tgaatcggtt ccccccacca gggcgagacc ggccggggcc acccacaaga cgatcgcggt
     3961 tccgaacgcc accgccgcgt ttcgttcgcc gcggctcatg cggccgaggc gtgcgcgttg
     4021 gcgcgcgaga aactcggaca cgccggtcag ggagccgaga tccgggggat gcagcgcgta
     4081 cagaagagca aaaagcgcgg cgaacgtcac gatcacgatc ggcatcccga actgcatcca
     4141 ttgcacgaac ccgatgtcca cgcccagtcg ttcctcgatg agccccttgc cgatcagatt
     4201 gggcggcgtg ccgatcacgg tccccagacc gccgatcgac gcgccgtacg ccaccatcaa
     4261 catgagtgcg gcgctgtagc gccatgcggc cggtgacgcg ggattgcccg cgggatccct
     4321 gcgcacgccc gccagcgcgc ccagcattcc gctcacaatc ggcaacatca tggcggccgt
     4381 ggcggtattg ctcacccaca gcgataatcc cgctgaaagg agcccgaccg ccagaagaat
     4441 tcgggcgggg ct
//
LOCUS       DBNGTT010000319         2144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_710031, whole genome shotgun sequence.
ACCESSION   DBNGTT010000319 DBNGTT010000000
VERSION     DBNGTT010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_710031"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1192
                     /gene="corA"
                     /locus_tag="AC16BC_07215"
     CDS             185..1192
                     /gene="corA"
                     /locus_tag="AC16BC_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR3241446.1"
                     /translation="MHIGTPSSQPTRIQVLRYGPDRIVEEPNATLDQALACRAEAGVA
                     WINVDGVQDVEVLKKLGEGYGLHPLVIEDIANTEQRPKLEDYGDHLFIVLKMLSPENG
                     HVRAEQVSVVITRNVILSFQEHDKPGDCFDPLRARLRADASRLRSAGADFLAYGIVDA
                     LVDNYFVVLEQLGDAVEHIEEAATLDPTASGAQSIHVLRRELLVMRRAVWPLRDLVAQ
                     LLRRESTLIGEKTAVYLRDVYDHLVQVIDTTEALRDLLSSLLEVELTRTSLRMNETMK
                     ALTLIATVFIPLTFIVGVYGMNFQYMPELRWRWAYPTLWALMIALGVGMVVWFKRKRW
                     L"
     gene            1273..>2144
                     /locus_tag="AC16BC_07220"
     CDS             1273..>2144
                     /locus_tag="AC16BC_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3241447.1"
                     /translation="MSEQTPERVREMVDGLYQAESRRVLATLIRLLGGDFDVAEEALH
                     DAFRSALEQWPRDGVPANPRAWLVSAGRFKAIDTMRRRARFESLDDVPEPIDAALPDP
                     TEWEQESVEDDRLRLIFTCCHPALSPDAQVALTLREVCGLTTEEIARAFLTPAPTLAQ
                     RIVRAKAKIRDARIPYQVPEPTELPDRLDAVLRVIYLVFNEGYAASSGASLTRHDLSG
                     EAIRLGRLLVELLPEPEAVGLLALMLLHDSRRAARTTPTGDLVLLEDQDRSLWNRDHI
                     AEGSALVSRALASR"
BASE COUNT          376 a          673 c          717 g          378 t
ORIGIN      
        1 agattcaatg tccgagaacc aacgcaacga tccgctaacc agccgctgca cgccaccgtg
       61 ttaagaaata cggcgcgtga gaggtagcga cgatcaaaga gacatcttcc gcaatgacac
      121 ccgacccatg aaacgtttga ctccacgccg tagtcgaaaa gccggtctgc cacccggtag
      181 tgtcgtgcac attggcacgc cgtcgtcgca gcccacgcgc atccaggttc tgcgttacgg
      241 cccggatcgg attgtcgagg aaccgaatgc cacgctggac caggcgctgg cctgtcgcgc
      301 ggaggctggc gtggcgtgga tcaatgtgga cggggtgcag gatgtggagg tgctcaagaa
      361 gctcggggaa gggtacgggc tgcacccgtt ggtgatcgag gacattgcca acacggagca
      421 gcggcccaag ctcgaagatt acggcgacca cctattcatc gtgctcaaga tgctctcccc
      481 tgagaacggt cacgtgcggg ccgagcaggt cagtgtggtc atcacgcgca acgtgatcct
      541 gtcatttcag gagcacgaca agcccgggga ctgctttgat ccgctgcgcg cgcgcctccg
      601 cgccgacgct tcgcgcctgc gcagcgcggg cgccgacttc ctggcctacg ggatcgtgga
      661 tgccctggtg gacaattatt ttgtggtgct cgaacaactc ggcgacgcgg tggaacacat
      721 cgaggaagcg gccacactcg atcccaccgc cagcggcgcc cagtcgatcc acgtgttgcg
      781 ccgcgagctg ttggtcatgc gccgcgcggt gtggccgctg cgtgatctgg tggcgcaatt
      841 gttgcggcgc gagtccacgc tgatcgggga aaaaaccgcg gtgtacctgc gcgatgtcta
      901 cgaccacctg gtgcaggtga tcgacaccac cgaggccttg cgcgatttgt tgtccagcct
      961 gctggaagtg gagctcactc gcaccagcct gcgcatgaac gagaccatga aggcgctcac
     1021 gttgatcgcg acggtgttca ttccgctcac gttcatcgtg ggcgtgtacg gcatgaactt
     1081 ccaatacatg ccggaactgc gctggcgatg ggcctaccca accctgtggg ctctcatgat
     1141 tgcgctcggc gtgggcatgg tggtctggtt caaacgcaag cgctggttgt agcgcgtgcc
     1201 ctgctcgcct tgagaatcgt cgggagtcag tgagaatatg ggggcggagc acggtcgtcc
     1261 taatccccct caatgagcga gcaaactccc gaacgcgtgc gcgagatggt ggacggcctg
     1321 taccaggccg aatcgcgccg cgtgctcgcc acattgatcc gtctccttgg cggagacttc
     1381 gatgtcgcgg aggaggcgct gcacgacgcg ttccggtcag cgctggagca atggccgcgc
     1441 gacggcgtgc ccgccaatcc acgggcgtgg ctggtgtcgg ccgggcggtt caaggccatc
     1501 gacaccatgc ggcggcgcgc gcggttcgag tcgctggacg acgtgcccga gcccatcgac
     1561 gcggcactcc ctgatcccac ggagtgggaa caggagagcg tggaggatga ccggctgcgg
     1621 ctcatcttca cgtgttgcca cccggcgctg tcgccggacg cgcaggtggc gctcacgttg
     1681 cgcgaagtgt gcgggctcac caccgaggaa atcgcccgcg cgtttctgac ccccgcgcca
     1741 actctggcgc agcggatcgt gcgcgccaag gccaagatcc gcgacgcgcg cattccgtat
     1801 caagtgcccg agccgaccga actgccggac cggctggatg cggtgctccg cgtcatctat
     1861 ctggtgttca acgaggggta cgccgcgtca tcgggcgcgt cgctgacccg gcacgatctg
     1921 tcgggcgaag cgatccggct gggacgattg ctggtcgagt tgctgcccga acccgaagcc
     1981 gttgggctgc tggcgctgat gctgctgcac gactcccgcc gcgcggccag gaccacgccc
     2041 acgggggatt tggtgctgct cgaggaccag gaccgctcgc tctggaaccg ggatcacatt
     2101 gccgaaggct cggcgctggt gtcgcgggcg ctcgcatcgc gcga
//
LOCUS       DBNGTT010000320         4829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_965967, whole genome shotgun sequence.
ACCESSION   DBNGTT010000320 DBNGTT010000000
VERSION     DBNGTT010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_965967"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /gene="tuf"
                     /locus_tag="AC16BC_07225"
     CDS             complement(<1..1181)
                     /gene="tuf"
                     /locus_tag="AC16BC_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR3241448.1"
                     /translation="MAKAKFERTKPHLNIGTIGHVDHGKTTLTAAITKVLADKKLAEF
                     KAYDQIDKAPEERERGITIAIAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDKELLDLVELEVRELLTK
                     YEFPGDKIPFVAGSALKALEGDKGPLGAEAILKLMANIDTYIPTPKREVDKPFQMSVE
                     DVFSISGRGTVVTGRIERGVVKVGEEIELVGIRPTQKTVVTGVEMFRKVLDQGQAGDN
                     VGILLRGTKKEDVERGMVLAKPGSITPHTKFKAEAYILTKEEGGRHTPFFNGYRPQFY
                     FRTTDVTGVAKLPEGVEMVMPGDNVRMEVDLITPIAMEEGLRFAIREGGRTVGAGVIT
                     G"
     gene            complement(1276..3357)
                     /gene="fusA"
                     /locus_tag="AC16BC_07230"
     CDS             complement(1276..3357)
                     /gene="fusA"
                     /locus_tag="AC16BC_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3241449.1"
                     /translation="MPRQFPLDRTRNIGIMAHIDAGKTTTTERILYYTGVTHRIGEVH
                     EGAATMDWMEQERERGITITAAATTCFWRDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFDSVQGVEPQSETVWRQADKYRVPRVAFMNKMDRIGADFFASVQSLVDRLGVRPV
                     CLQLPIGKESAFRGPVDLIEMKGVFFDDETLGAKFVVSDIPADMAEQAAEYREKMVEA
                     LADCDERVMERYLAGESPTVEELKAAIRRGVMEMKIVPVLCGAAFKNKGVQLLLDAVV
                     DYLPSPVDIPPVVGIDVKTSAEVTRRADDDEPFAALAFKIMTDPFVGQLTFFRVYSGV
                     LRAGSYVYNATKDTKERIGRILKMHANKREEVKEMYAGEIGAAVGLKGATTGDTLCDE
                     TKPILLEVMKFPEPVIQMAIEPKTKQDQEKMGLALQKLAQEDPSLRVSTDEETMQTLI
                     AGMGELHLEIIVDRMKREFKVEANVGKPQVAYRETIRTASTAEGKYIRQTGGRGQYGH
                     VVLELEPQEPGKGFEFENKLVGGAVPREYVPAVEKGIVEAMEGGVVAGYPMVDLKVAI
                     VDGSYHDVDSSEMAFKIAASMGFKEAARKANPVLLEPIMSLEVVVPEDYMGDVIGDIN
                     SRRGKIQGMKGRGTAQVITAEVPLSEMFGYATDLRSKTQGRGIFTMQFAHYEMVPKPI
                     ADQIVAKVQGG"
     gene            complement(3526..3999)
                     /gene="rpsG"
                     /locus_tag="AC16BC_07235"
     CDS             complement(3526..3999)
                     /gene="rpsG"
                     /locus_tag="AC16BC_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR3241450.1"
                     /translation="MPRRRVPARRESMSDPRYNDRLVGKFINTLMKKGEKSVAESVCY
                     DAFDLIKTKTNGGDPLKFFRSAIDNIKPVVEVKSRRVGGASYQVPVEVRQDRRISLAL
                     RWLVSYAKQRSGRSMEEKLASELLDASNNTGGAVKKKEDTHKMAEANKAFAHYRW"
     gene            complement(4046..4417)
                     /gene="rpsL"
                     /locus_tag="AC16BC_07240"
     CDS             complement(4046..4417)
                     /gene="rpsL"
                     /locus_tag="AC16BC_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR3241451.1"
                     /translation="MPTINQLVRQGRFQARSKTKSPALKGAPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DTQGVQNRKQGRSKYGAKTPK"
     gene            complement(4550..>4829)
                     /locus_tag="AC16BC_07245"
     CDS             complement(4550..>4829)
                     /locus_tag="AC16BC_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241452.1"
                     /translation="ASLSTDSFISAASFQETTRVLTEAAITGRVDDLLGLKENVIVGR
                     LIPAGSGLADYRDTFVEAPIGFQEPGAAQGVEGATEVAVETGEFERAS"
BASE COUNT          815 a         1716 c         1295 g         1003 t
ORIGIN      
        1 atgccggtga tgacgccggc gcccacggtg cggccgccct cgcggatggc aaaccgcagc
       61 ccctcttcca tcgcgatcgg cgtgatcagg tccacttcca tccgcacgtt gtcccccggc
      121 atcaccatct ccacgccctc cggcagcttc gccacccccg tcacgtccgt ggtccggaag
      181 taaaactgcg gccggtaccc gttgaagaac ggcgtgtgcc gccccccttc ttctttggtc
      241 aagatgtacg cttccgcctt gaatttggtg tgcggcgtga tgctcccggg tttggccagc
      301 accatgcccc gctccacgtc ttctttcttg gtgccccgca gcaggatccc cacgttgtcc
      361 cccgcctgcc cctggtccaa cactttgcgg aacatttcca cgcccgtgac caccgtcttt
      421 tgcgtcggcc ggatccccac cagctcgatt tcttccccca ctttcaccac gccccgctcg
      481 atccgccccg tcaccacggt cccccgcccc gagatcgaga agacgtcttc caccgacatc
      541 tggaacggtt tgtccacttc ccgcttcggc gtcgggatgt aggtgtcgat gttggccatc
      601 aacttcagga tcgcttccgc ccccaacggg cccttgtccc cttccaaggc tttcagcgcg
      661 ctgcccgcca cgaacgggat tttgtccccg ggaaattcgt atttggtcaa gagctcccgc
      721 acttccaact ccaccaggtc caggagctct ttgtcatcca ccatgtcggc tttgttcaag
      781 aacaccacga tgtacggcac ccccacctgc cgcgccaaca ggatgtgctc gcgcgtttgc
      841 ggcatcgggc cgtccgccgc gctgaccacc aggatcgccc cgtccatttg cgccgcgccc
      901 gtgatcatgt tcttcacgta gtcggcgtgc cccgggcagt ccacgtgcgc ataatgccgg
      961 ttctcggtct cgtactccac gtgcgcgatg gcgatcgtga tgccccgctc gcgctcttcc
     1021 ggcgctttgt cgatctggtc gtaggccttg aactccgcca gtttcttgtc cgccagcact
     1081 ttcgtgatcg ccgccgtcaa cgtggtcttc ccgtggtcca cgtgtccgat cgtcccaatg
     1141 ttcaggtgcg gcttcgttcg ctcaaatttc gctttcgcca tcgtcgtctc ctgacttcgt
     1201 tattcgttgg tagttgttcg ttgttcgcac gcccaacaat caaccgacaa caaacaacgc
     1261 gcttcgtcct ctcccttatc cgccttggac cttcgccacg atctggtcgg caatcggttt
     1321 aggcaccatt tcatagtggg cgaactgcat ggtgaagatg cctcgcccct gggtcttgga
     1381 tcgcagatcc gtggcgtacc cgaacatttc ggacaacggc acctccgcag tgatgacctg
     1441 cgcggtgccg cggcccttca tgccctggat cttgccgcga cgcgagttga tgtcgccgat
     1501 gacgtcgccc atatagtctt caggcaccac cacttcgagc gacatgatgg gttccagcag
     1561 gaccggattc gctttccggg ccgcttcctt gaagcccatc gacgcggcga tcttgaacgc
     1621 catttccgag gagtccacgt cgtgatagga cccgtccacg atggcgacct tgaggtccac
     1681 catgggataa ccggccacca cgccgccctc catcgcctcc acaatgcctt tctcgaccgc
     1741 aggcacgtac tctctcggca ccgcgccgcc caccagcttg ttctcgaact cgaatccctt
     1801 cccgggttcc tgcggttcga gctccaagac cacatggccg tactgcccgc gccccccggt
     1861 ctgccggatg tacttgccct ccgccgtgga cgcggtccga atcgtttcgc ggtaggccac
     1921 ctgcggcttc ccgacgttgg cttcgacctt aaattcgcgc ttcatgcgat cgacgatgat
     1981 ctcaaggtgc aactcgccca tcccggcgat caaggtctgc atggtttctt cgtcggtgga
     2041 cacgcgcagc gaggggtctt cctgcgcgag cttttgcagc gcgagcccca tcttctcctg
     2101 atcctgcttg gtcttgggct cgatcgccat ttgaatcacg ggctcgggaa acttcatgac
     2161 ctcgagcaga atcggcttgg tctcgtcgca gagcgtgtcg ccggtcgtgg cgcccttgag
     2221 cccgacggct gcgccgatct cgcccgcgta catctccttg acctcttcac gcttgttggc
     2281 gtgcatcttc aaaatacggc cgatgcgctc cttggtgtcc ttggtcgcgt tgtacacgta
     2341 ggagcccgcg cggagcacgc cggagtacac ccggaagaac gtgagttgcc ccacgaacgg
     2401 gtcggtcatg atcttgaacg ccagggcggc aaacggctcg tcgtcgtccg cgcggcgcgt
     2461 cacctcggcc gacgtcttca cgtcgatccc cacgaccggg gggatatcga ccggcgacgg
     2521 cagatagtcc accaccgcgt ccaacagcaa ctgcacgccc ttgttcttga atgccgcgcc
     2581 gcacagcacc ggcacgattt tcatctccat gacgccgcga cggatcgccg ccttcaactc
     2641 ttcgacggtc ggggactcac ccgccaggta gcgctccatc acccgctcgt cgcaatcggc
     2701 cagggcttcg accattttct cgcggtactc ggcggcctgt tcggccatgt cagccgggat
     2761 gtcgctgacc acgaatttgg cgcccagcgt ctcgtcgtcg aaaaacacgc ccttcatctc
     2821 gatcagatcg accggcccgc ggaacgcgct ttccttcccg atcgggagct gcagacacac
     2881 cgggcgcacg cccaaccgat ccaccagcga ctgcacgctc gcgaaaaaat cggcgccgat
     2941 gcggtccatc ttgttcatga acgccacgcg gggcacgcga tacttgtccg cctggcgcca
     3001 cacggtttcg gactgcggct ccacgccctg caccgagtcg aacaccgcga ccgcgccgtc
     3061 cagcacgcgg agcgagcgct ccacttcaat ggtgaagtcc acgtggcccg gggtatcgat
     3121 gatgttgatg cgatggtccc gccagaaaca ggtggtcgcg gcggcggtga tcgtgatgcc
     3181 ccgctcgcgc tcctgctcca tccagtccat ggtggccgcg ccctcatgaa cctcgccgat
     3241 ccgatgggtc accccggtgt aatagaggat gcgttccgtg gtcgtggttt tgcccgcgtc
     3301 gatatgcgcc ataatcccga tattgcgggt ccgatccaat ggaaactgac gaggcacgac
     3361 aagacgctcc ttacgttaag ccacgacaat caacagagac accaatgcag ggccagggaa
     3421 cccattggcc cctgaatccc cacgttcccc gaactatatt gatcgagcga attaccgccc
     3481 gaacgaaccg atcacgccga tactacggaa gaaccgggaa ggccgttacc accgataatg
     3541 cgcaaacgcc ttatttgcct ccgccatctt gtgcgtgtct tccttcttct tgaccgcgcc
     3601 gccggtgttg ttcgaagcgt ccagcaattc cgaggcgagc ttctcctcca tggagcggcc
     3661 gcttcgctgc ttggcgtacg agacgagcca ccgcaacgcc aacgaaatcc gccgatcctg
     3721 acggacctca accggcacct gatacgacgc gccgccgacg cgccgggact tcacttccac
     3781 caccggcttg atgttgtcga tcgcggaccg gaagaacttg agggggtcgc ccccgttggt
     3841 cttggtcttg atcaggtcga aggcgtcata acacaccgac tccgcaacgc tcttctcgcc
     3901 ctttttcatc agggtgttga tgaacttgcc gacaagccga tcgttgtacc gcggatcgga
     3961 catggactcg cgccttgccg gcaccctacg ccgcggcatc gaacactccc tttctctgca
     4021 tcatgactcg atccacttcc gcgccctact tgggcgtctt ggcgccgtac ttcgagcgcc
     4081 cttgcttgcg attctgcacg ccctgcgtat ccagggtgcc gcgcacgatg tggtaccgca
     4141 cgccggggag atccttcaca cggccgccgc gcaccagcac gatcgagtgc tcctgcaggt
     4201 tgtgccccac gccgggaatg tacgtcgtca cttccatccc gttcgtaagc cgcaccctcg
     4261 ccaccttccg caacgcggag ttcggctttt tcggcgtcgt cgtgtagacg cgcacgcaca
     4321 ccccgcgacg ctggggcgcg cccttcaacg cgggagactt ggttttggac cgagcctgga
     4381 accgcccttg tctgaccaac tgattgatcg tcggcacgct atccctcaac tcaatgcgtc
     4441 gtgatggacg ccgacaacgg cgcgatcacc tgacgctggc gaaaaacttt ggaagtatag
     4501 tgatttacca ctcccatgtc aagcaatttc ctcccgaacg acaccgaact cacgacgcgc
     4561 gctcgaactc tcccgtctct accgcaacct cggtggcgcc ctcgactccc tgcgcggcgc
     4621 ccggctcctg gaagccgatc ggcgcctcca cgaacgtgtc gcgataatcg gccaacccac
     4681 tcccggccgg gatcaaccga ccgacgatca cgttctcctt gagacccaac aagtcgtcga
     4741 cccggcccgt gatggcggct tcggtcagca cccgcgtcgt ttcctggaag gacgcggcgg
     4801 agatgaagct gtcggtcgac aacgacgcc
//
LOCUS       DBNGTT010000321         3397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3392577, whole genome shotgun sequence.
ACCESSION   DBNGTT010000321 DBNGTT010000000
VERSION     DBNGTT010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3392577"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..809
                     /locus_tag="AC16BC_07250"
     CDS             312..809
                     /locus_tag="AC16BC_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3241453.1"
                     /translation="MTGPAFTTLLVPTDFSQGAAAAVEYALWLAGRPHATVIFFHVME
                     PTVYSVDFALTRPDLSAEVRERAQDVLRQLVEEARGRGIPADQAVAVGAPFAEIRKAA
                     ADRRVDLIVMGTHGRTGAAHVVLGSTAERVVRLAPCPVLTVKASGKSTTGTPASADPA
                     ASRNR"
     gene            873..1856
                     /locus_tag="AC16BC_07255"
     CDS             873..1856
                     /locus_tag="AC16BC_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3241454.1"
                     /translation="MANGTCGCSTRCGIVLAGGAGQRLRSFVYRLRGDALPKQYVRFT
                     GSRSLLEQTLDRVERIVPPERVFTVATMSHLQFPDAQGQLECRPPGTVILQPENRETA
                     PGVLLPLVHLEARYPNALVAVFPSDHFIDPEGLFMGYVDLAFRAVGAHPGRLVLLGVK
                     PDRAEPEYGYIVPEPLRPGRAGSGVRRVSSFVEKPASDSVAELALRGGLWNTMVMVFR
                     PRTVMDAVARVAPALHRAFDHVRRAFGTSQEARVVEEVYRDIEPANFSRTVLEAIPGR
                     RSPSLVVLPVRGVYWSDWGSEQRIANTLTRTTERPFAVQGASNTDIRAEAV"
     gene            1863..2876
                     /locus_tag="AC16BC_07260"
     CDS             1863..2876
                     /locus_tag="AC16BC_07260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3241455.1"
                     /translation="MSTATGPGDSIRVVLGGDVMLGRLVKEEILARGPRYPLGPVAEF
                     MRGADLTVVNLECAITSRETVWEGASKAFYFGAPPGAVESLADAGVDLVSLANNHSLD
                     FGRRGLLDTLDGLRERGIAAAGAGRTLDDAEAPAVVERRGVRFGMAAFCDHQADFAAR
                     ADAPGIAFLDLLDERAACRRFQERLDRMRALGAEWPILSLHWGPNMVWRPSSRFVRLA
                     RAAIDMGYASVFGHSAHVFQGIELYKGRPIFYSVGDLVDDYAVDPDFENDRQLLFEVS
                     LTGLAVREVALYPVVIDQCRTDWAAGAAFDRIAERATGLSAEMGTTVTRDGNRLVATV
                     SGG"
     gene            2878..>3397
                     /locus_tag="AC16BC_07265"
     CDS             2878..>3397
                     /locus_tag="AC16BC_07265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3241456.1"
                     /translation="MRRAGTEKPASAVRVDGGDGVTLDGEWCIPEQARGIVAFAHGSG
                     SSRHSPRNQFVARALREAGLGTLLFDLLTAREDAVYETRFDIELLAKRLVAATTWLHR
                     QPAARSLGVGYFGASTGAAAALIGAARSGAAVKAVVSRGGRPDLAEAALSLVTAPTLL
                     IVGGLDDVVIDLN"
BASE COUNT          477 a         1090 c         1267 g          563 t
ORIGIN      
        1 agggatggcg agcaccaacg ggtgccgtcc ccgatactct cccaggcgat ccgccaggag
       61 tcgtgcggca tggtcgcggt ttcggaaggt catggacatc ttttcggacg gcattgtagc
      121 ctaagacggc cccgcgcgac tagccctcta ttggcgtagg agcccgaagt aaaaagcgtg
      181 agggggcggc gcagacgggc catgcgggtt tgcccggttt ccccgatgca ccccccccgt
      241 ttgggggatg ggttttgtcc cggacatgtg tacaatgggc tcgccgcata atccaagtcg
      301 ttgggagcac catgaccgga cccgcgttca ccacgctgtt agtcccgacc gatttctccc
      361 aaggcgcggc cgccgcggtc gagtatgcgc tctggttggc cggccgaccc cacgccacgg
      421 tgattttctt ccacgtcatg gagcccacgg tctactccgt tgatttcgcg ttgacccggc
      481 ccgacctctc cgccgaggtc agggagcggg cgcaggacgt attgcgccag ttggtggagg
      541 aggcgcgcgg tcgcggcatc cccgcggatc aggcggtggc ggtcggggcg ccgttcgcgg
      601 aaatccgcaa ggccgcggct gatcgacgcg tcgatctgat tgtcatgggc acccacgggc
      661 gaaccggggc ggcgcacgtg gtgttgggca gcacggccga acgcgtggtg cgcctggcgc
      721 cctgtccggt gttgaccgtg aaagcgtccg ggaaatcgac gaccgggaca ccggcatcgg
      781 cggaccccgc ggcgtcacgc aatcgctgaa gccttccggg gcgatcgcgc cccggcgtcg
      841 gttcgcggca cccaaagagg agggagttcg tgatggcgaa cggaacctgc gggtgctcca
      901 cgcggtgcgg gatcgtgctg gccggcggcg cgggccaacg actgcgctcc ttcgtgtatc
      961 gcctccgcgg cgatgccttg cccaagcagt acgtgcgttt caccggatcg cggtcgctgc
     1021 tcgaacagac actcgatcgg gtggaacgga tcgtgccgcc cgagcgggtg ttcacggtgg
     1081 ccacgatgtc gcatctgcag tttcccgacg cccagggcca gctcgagtgt cgcccgcccg
     1141 gcaccgtgat cctgcaaccc gaaaatcggg aaaccgctcc gggcgtcctc ctgccgctgg
     1201 tccacctcga ggcgcggtac ccgaacgcgc tggtcgcggt gtttccgtcc gaccacttca
     1261 tcgatcccga gggcctcttc atgggctacg tggacctggc gtttcgcgcg gtgggggctc
     1321 atcccggtcg tctcgtgctg ctgggggtaa aacccgatcg cgcggaaccc gagtacggct
     1381 acatcgtccc ggaaccgctc cggccggggc gggccgggtc gggcgtccgc cgggtgtcct
     1441 cgttcgtcga aaaacccgcg tccgattcgg tcgcggagct ggcgttgcgc ggggggctct
     1501 ggaacaccat ggtgatggtg ttccggccga gaaccgtgat ggacgcggtg gcgcgggtcg
     1561 ctcccgccct gcatcgcgcg ttcgatcacg tgcgccgggc gttcgggacg tcccaggagg
     1621 cgcgggtggt ggaggaggtg taccgcgata tcgagcccgc gaacttttcc cggacggtgc
     1681 tggaggcgat tccgggtcgg cgctcgccgt cgctggtggt gttgccggtc cggggcgtct
     1741 actggagcga ttggggctcg gagcagcgga tcgcgaatac gctcacgcgg acgaccgaac
     1801 ggccgttcgc cgttcagggc gcgtcgaata cagacatccg agcggaggcc gtctgacgcg
     1861 ccgtgagcac ggcgacgggt ccgggcgatt cgatccgcgt ggtgctgggc ggggacgtga
     1921 tgttgggccg gctggtgaag gaagagatcc tggcccgcgg cccccggtat ccgctgggcc
     1981 cggtggcgga attcatgcgg ggcgcggacc tcaccgtcgt gaacctggag tgcgcgatca
     2041 cgagccggga gacggtgtgg gagggcgcgt ccaaggcgtt ttatttcgga gcgccgccgg
     2101 gcgcggtgga gtcgctggcg gacgcgggcg tggacctggt gagcctggcc aacaaccaca
     2161 gcctggactt cggccgacgc ggcctgctcg acacgttgga cgggttgcgc gagcgcggca
     2221 tcgcggcggc tggcgcgggc cgcacgctgg atgacgccga ggcgccagcc gtggtcgagc
     2281 gacgcggcgt gcggttcggc atggccgcgt tctgcgacca tcaagcggac tttgcggcgc
     2341 gggcggacgc gcccggcatc gcgtttctcg acctgctcga cgaacgcgcc gcgtgccggc
     2401 gcttccagga gcgtctcgac cgcatgcgcg cgctcggggc ggagtggccg atcctgtcgt
     2461 tgcactgggg cccgaacatg gtgtggcggc cctcgtcgcg gtttgtccgg ttggctcgcg
     2521 ccgcgatcga tatggggtat gcgtcggtgt tcgggcacag cgcgcacgtg ttccagggga
     2581 tcgaactcta caagggccgt ccgattttct actccgtcgg agacctggtc gacgactacg
     2641 cggtggatcc cgatttcgaa aacgaccgcc agctcctgtt cgaagtctcg ctcaccggcc
     2701 tcgcggtcag ggaggtggcg ctctacccgg tcgtcatcga ccagtgccgg acggactggg
     2761 ccgccggggc cgcgttcgat cggatcgccg aacgcgcgac cgggctgtcc gccgagatgg
     2821 gaacgacggt gacccgtgac gggaatcggc tcgtcgcgac cgtgagcgga ggatgagatg
     2881 aggcgcgcgg gaaccgagaa gccggcgagc gcggtccgcg tggacggcgg agacggcgtc
     2941 acgctcgatg gagaatggtg catcccggag caggcccggg gaatcgtcgc cttcgcgcac
     3001 ggaagcggca gcagccggca cagccctcgc aaccaattcg tcgcccgcgc gttgcgcgag
     3061 gccgggctcg gtaccctgct gttcgacctg ctgaccgccc gtgaggacgc ggtctacgag
     3121 acgcgcttcg acatcgagtt gttggccaag cggctggtcg ccgcgacaac gtggctgcac
     3181 cggcagccgg ccgcccgctc gctcggcgtg gggtatttcg gggcgagcac cggagcggcc
     3241 gcggcgctga tcggggcggc gcgatcgggc gcggcggtca aggccgtggt atcccggggc
     3301 gggcggccgg acctggccga ggcggccttg tccctggtga ccgctcccac cctcctgatc
     3361 gtgggcgggc tggacgacgt ggtgatcgac ctcaacc
//
LOCUS       DBNGTT010000322         4918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3010296, whole genome shotgun sequence.
ACCESSION   DBNGTT010000322 DBNGTT010000000
VERSION     DBNGTT010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3010296"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="AC16BC_07270"
     CDS             <1..1572
                     /locus_tag="AC16BC_07270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR3241457.1"
                     /translation="VRHPEYEQFLEENWYFEAVSDTYLPVLDALERLAADNVRARITM
                     TLSPSLTAMFDDALLRGRVVRYFDQRISLARSEERRLGGDAAFGPLAAYYRDRFERSR
                     DAFVNRYHQDLTGAFRGFQDQGTLEIITCGATHGFLPLLGIVPSAARAQVRVAVDSYR
                     RAFGRDPRGIWLPECAYAPGFDEVLRDEGIRFFMMESHGLAHAKPRPRFGLHAPVYCP
                     SGVAAFARDVESSKQVWSANEGYPGDFAYREYYRDVGFDLPVERLREHLPHLGPRYNT
                     GIKYYRITGRTDHKEAYVPAQARERAADHAGNFLFNRGQQIDYLAGGMDRPPLVVAPY
                     DAELFGHWWFEGPIWIEMLCRKLHFDQEQIEPVTPSEYLERHPTNQVVEPCASSWGHN
                     GYYEVWLNGANDWIYRHLHHASKRMDTLAARDPAPGGLEHRAVTQALRELLLAQASDW
                     AFIMKTGTMVPYAIKRTTDHLLRFLRLADQIDSGTIDQAWLADIEARDTCFPHLDVAA
                     AYRPERARAARSATW"
     gene            1569..3653
                     /gene="rnr"
                     /locus_tag="AC16BC_07275"
     CDS             1569..3653
                     /gene="rnr"
                     /locus_tag="AC16BC_07275"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR3241458.1"
                     /translation="MSARRRSGTRPRLRGTDIVTGLLQGHPDGFGFVIPDEDPALGRR
                     DVYIPARAMADAMHGDRVEARIEPRKPQARGRQMRARDEDRRQGRILRVLERTRRRVV
                     GRLTHGPAFLFVVPTDPRISRDVAILPADLHGARDGDLVVAEITAYPEGRQGFFGHVV
                     QVLGRSGDPALDTDMVIAEFDLPREFPPEVEREAHAIPDRLTAGLLAGRRDLRALATV
                     TIDGEHAKDFDDAVSIEALPHGGFRLWVHIADVSAYVSWDSPLDAEARRRGTSVYFPD
                     RVVPMFPERLSNGLCSLNPGEDRLTLTCEMAFDAGGHRTGYALYPSVIISDERMTYTA
                     VGRILEQEDADLSRRYSSLLPMFRTMRALCEQLSLRRRERGSIDFDLPEPEILLDVLG
                     ETAAILKHPRTIAHRIIEEFMLAANETVAQHLDRLRIPSVYRVHEQPDPDKLLNFAQI
                     ASGFGHRVSVGAPIHPKPLARAVEAVRGRPEEALLNTLLLRSMKQARYAVENRGHFGL
                     AATHYTHFTSPIRRYPDLVVHRLVHAALSGGGLPTERLEALLPEIALWSSSRERLAME
                     AEREVVDLKKARFMADKVGDEFSGVISGVTAYGFFVQLTDVFVEGLVRAETIEDDYYV
                     LDEARHALVGRRHRREFRLGDPTRVMVDRVDLIRRQVDFRLAVTGEFRSAPAPPRRGR
                     SRASAKPRRRRD"
     gene            3769..>4918
                     /locus_tag="AC16BC_07280"
     CDS             3769..>4918
                     /locus_tag="AC16BC_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241459.1"
                     /translation="MDESLPKSSPLNPRDLTRVLTDLQVAIRKLSMYSAAHPIVPGIV
                     ASLTKQLQTLTGSYGAFSVGITKDEVLYDATPVASGNPVIRELARLLNQLNIAGVGFQ
                     VNLTEPHVQRFLELLAGSRGLATSAERESALEQYCRTAPAITLQFISFRGAVRDRDDS
                     ESGAELTGFQLWRGLVSRLTADGLPEQSRAALEAAAANPEDVERLASVIALISQHQKS
                     GAGSYEQTIVKYLQHQSTDLPEKGTARATFNQEISRLFTNLPAEVRQQILRTSLEAGD
                     GATTPVESFLDALPTPVMLEVLDQVRGAGRDVSLPTLSLLKKFVTLAESNDSLTVALR
                     DKLPDHRDVLQDLLTKRANRTFYPSQYRALLDEDFSERAVSASGVARQE"
BASE COUNT          847 a         1644 c         1572 g          855 t
ORIGIN      
        1 gtgcgccacc cggaatacga acagtttctg gaggagaact ggtatttcga agccgtctcc
       61 gacacctacc tgcccgtgct cgacgcgctc gaacgcctgg cggccgacaa cgtgcgcgcc
      121 aggatcacca tgaccctgtc cccttccctc accgcgatgt tcgacgatgc gcttctccgc
      181 ggccgcgtgg tgcgatactt tgatcaacgc atctcgctgg cgcgctcgga ggaacgccgg
      241 ttgggcggcg acgccgcgtt cgggccgctc gccgcgtatt atcgcgaccg gttcgagcgc
      301 agccgcgacg cgttcgtcaa ccggtatcac caggacctga ccggcgcgtt ccgcggcttt
      361 caagatcaag gcaccctgga gatcatcact tgcggggcca cgcacgggtt cctgcccttg
      421 ttgggcatcg tgccgagcgc cgcgcgcgcc caggtgcgcg tggccgtcga ctcgtaccgc
      481 cgcgccttcg gccgcgatcc gcgggggatc tggctgcccg agtgcgcgta tgccccgggt
      541 ttcgacgagg tgctgcgcga cgagggcatt cgatttttca tgatggagag ccacggcctg
      601 gcgcacgcca aaccgcgtcc gcgcttcggg ctgcacgcgc ccgtgtattg cccgtccggc
      661 gtggccgcgt tcgcacgaga cgtggagtcg tccaaacagg tctggagcgc gaacgagggc
      721 taccccggcg atttcgcgta ccgcgaatac taccgcgacg tgggcttcga cctgccggtg
      781 gaacggctgc gcgagcacct gcctcacctg ggcccgcgct acaacaccgg catcaagtac
      841 tatcggatca cgggccgcac cgaccataaa gaggcctacg tcccggcgca ggcccgtgaa
      901 cgcgcggccg accacgcggg caactttctg ttcaaccgcg ggcagcagat cgactacctg
      961 gcgggcggca tggatcgtcc gccgctcgtg gtggcgccgt acgacgccga actcttcggg
     1021 cactggtggt tcgaggggcc catctggatc gagatgttgt gccggaagct gcacttcgac
     1081 caggaacaga tcgaacccgt caccccgtcg gaatacctgg agcgccaccc caccaaccag
     1141 gtcgtggagc cctgcgcatc gagttgggga cacaacggat attacgaggt ctggctcaac
     1201 ggcgccaacg actggattta tcgccatctg caccatgcct cgaagcgcat ggacacgctc
     1261 gcggcgcgcg atcccgcgcc cggcggcctg gaacaccgcg ccgtcacgca ggcgctgcga
     1321 gagttgctgt tggcccaggc gtccgattgg gcgttcatca tgaagaccgg caccatggtg
     1381 ccctatgcga tcaaacgcac gaccgatcac ctgctgcggt ttcttcgcct ggcggaccaa
     1441 attgattcgg gaaccatcga ccaggcttgg ctcgcggaca tcgaagcgcg cgacacgtgt
     1501 ttcccgcatc tcgacgtcgc ggccgcgtac cgacccgaga gagcgcgcgc cgcgcggtcc
     1561 gcaacttggt gagcgcccgg cgccgaagcg gaacccgtcc gcgcttacgg ggcaccgaca
     1621 tcgtcacggg cctgctccaa ggccacccgg acggattcgg attcgtgatc ccggacgaag
     1681 accccgcgct ggggcgccgt gacgtctaca ttccggcgcg cgccatggcc gacgccatgc
     1741 acggcgaccg cgtggaagcc cggatcgagc cacggaagcc ccaggcacgc ggccgccaga
     1801 tgcgcgcacg ggacgaggac cgccggcaag gacggatcct gcgggtactg gaacggacca
     1861 ggcgccgggt ggtgggacgg ctcacgcacg gcccggcctt cctgttcgtg gtgccgacgg
     1921 atccgcggat cagccgggat gtggcgatcc tcccggctga tcttcacggc gcgcgagacg
     1981 gcgacctggt ggtcgccgag atcaccgcgt accccgaggg tcgccagggg tttttcggcc
     2041 acgtcgttca ggtactcgga cgttcgggcg accccgcctt ggacaccgac atggtcatcg
     2101 cggagtttga tttgccgcgc gaattcccgc cggaggtcga gcgcgaggcg cacgcaatcc
     2161 cggatcggct cacggccggt ttattggcgg gccgacgcga cctgcgcgcg ttggccacgg
     2221 tcacgatcga cggcgaacac gcgaaggatt tcgacgacgc ggtgtcgatc gaggcgttgc
     2281 cgcacggggg ctttcgcctg tgggtgcaca tcgcggacgt gtccgcctac gtgtcgtggg
     2341 acagtccgct ggacgcggaa gcccgccgtc gcgggaccag cgtgtacttc ccggatcgcg
     2401 tggttcccat gttcccggag cggttgtcca acggattgtg cagcctcaac cccggcgagg
     2461 accgcctcac actcacctgc gaaatggcgt tcgacgcggg cggacatcgc accggctacg
     2521 cgctgtatcc cagcgtgatc atcagcgacg aacggatgac ctacaccgcg gtcggacgaa
     2581 tcctggagca ggaggacgcg gacttgagtc gtcggtattc gtcgctgctg cccatgttcc
     2641 gaaccatgcg cgcgttatgc gagcaattgt ccctgcgtcg gcgcgagcgc gggagcattg
     2701 atttcgatct gcccgagccg gaaatcctgc tggacgtgct cggcgagacc gccgcgatct
     2761 tgaagcaccc gcgcaccatc gcgcaccgaa tcatcgaaga attcatgctc gcggcgaacg
     2821 aaaccgtggc gcaacacttg gatcgattga ggattccgtc ggtgtatcgc gtacacgagc
     2881 agcccgaccc cgataagctg ctgaacttcg cgcaaattgc gtcgggattc ggacaccgcg
     2941 tctcggtggg cgccccgatc caccccaaac ccctggcgcg cgcggtggag gcggtccgcg
     3001 gacgtcccga ggaagccctg ctcaacacgc tcctcctgcg ctcgatgaag caagcgcggt
     3061 acgcggtgga gaaccgcggt catttcggtc ttgccgccac gcattacacc cactttacct
     3121 caccaatccg tcggtaccct gatttggtcg tccatcgctt ggtgcatgcg gcgctatccg
     3181 gcggcgggct tcccaccgaa cggctggagg cgctgctgcc ggaaatcgcg ctgtggtcct
     3241 cgtcgcgcga gcgtctggcc atggaggccg agcgcgaggt cgtggacctc aagaaggcgc
     3301 gcttcatggc ggacaaagtc ggcgacgagt tctccggcgt catctccgga gtcaccgcat
     3361 acggcttctt cgtccagctc accgacgtgt tcgtggaagg tctggtgcgc gccgaaacga
     3421 tcgaggacga ctattatgtt ctcgacgaag cccgccacgc cctcgtgggt cgtcgacacc
     3481 ggagagagtt ccgactgggt gatccgacgc gtgtgatggt ggaccgcgtg gatctcattc
     3541 ggcgacaagt ggatttccgg ttggccgtta ccggtgaatt ccggtcggct cccgcgcccc
     3601 ctcgacgcgg acggagcagg gcgtccgcga aacctcgtcg gcgacgcgac tagggaagcg
     3661 cctccccgac ggggtcattc ttccggcgtc cggacccggc cctgcctgag ttgacaggca
     3721 tcggatcagc ggtactgttg ttcccgccgt attcgaggac gtctccgggt ggacgaatca
     3781 cttccgaaaa gttcgccgct caatccccgt gatttaacgc gcgtcttgac cgacctccag
     3841 gtcgccatcc gcaagctctc catgtattcg gcggcgcacc ccatcgttcc cggaatcgtc
     3901 gcgtcgttga ccaagcagct ccaaacctta accggctcat acggcgcgtt tagcgtcggc
     3961 attaccaagg atgaggtctt gtacgacgcc acgcccgttg cgagcggcaa cccggtcatt
     4021 cgcgaactcg ctcgcctgtt gaatcaactc aacatcgccg gcgtgggttt ccaggtcaac
     4081 ctcacggagc cccacgtcca acgttttttg gagcttctgg cggggtcccg cgggctggcc
     4141 acgtccgctg aacgcgagag cgcgctcgaa cagtattgcc gaaccgcgcc cgccatcacg
     4201 ctgcagttca tcagttttcg gggagccgtc agggaccgag acgactcgga gagcggagcg
     4261 gagctgacgg ggtttcaatt gtggcggggc ctggtgagcc gcctcacggc cgacggactc
     4321 cccgaacagt cccgagccgc cctcgaggca gccgccgcca accccgaaga tgtcgagcgg
     4381 ttggcctcgg tcatcgcctt gatcagccag catcagaagt cgggcgcagg gtcgtacgag
     4441 cagaccatcg tcaagtacct gcagcatcaa tcgaccgatc tgccggaaaa ggggaccgcg
     4501 cgcgccacgt ttaaccagga gatcagccgc ctgttcacga atctccctgc cgaagtacgc
     4561 cagcagattc tccgaacctc gctcgaggcg ggcgacggcg caaccacccc ggttgaatcg
     4621 ttcctggacg cgctccccac ccccgtgatg ctggaagttc tcgaccaggt tcgtggcgcg
     4681 ggacgcgacg tgtcgctccc cacgctgagc ctgttgaaga aattcgtcac tttggctgaa
     4741 tcgaatgaca gcttgactgt ggcattgcga gacaagttgc cggatcatcg agatgtgttg
     4801 caggacctcc tcaccaaacg cgcgaaccgc acgttttatc cctcgcaata ccgggcgctg
     4861 ctcgacgaag acttttcaga acgcgcggtg tccgcgagcg gggttgcgcg gcaggaaa
//
LOCUS       DBNGTT010000323         4089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3393081, whole genome shotgun sequence.
ACCESSION   DBNGTT010000323 DBNGTT010000000
VERSION     DBNGTT010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3393081"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="AC16BC_07285"
     CDS             <1..1487
                     /locus_tag="AC16BC_07285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3241460.1"
                     /translation="SEISNAMAPPRSTGDYQRLAEAADAAARSPWKTRGFLEFGAAAL
                     LGAIAAMAWVALSTKADFPPARARDIRPIAGRPSHLPRWGGKVARARSATLSIWPREV
                     IIGTHCTRDIGPHAARENATHVTILTDQGVSRKGLVDPVKASLTEAGVMCEVMDQVSR
                     EVPHTVIAEIAGRCKEAGTDLLIAVGGGSVIDTAKAVGIVLTNGGSIRDYEGVDRVRR
                     PIAPLYAVPTTAGCGAETSPFCIVRDATKKRKIEIFSRKLIPERIFIDPLMTRLMPPD
                     LTASSGMDALANAVEAYCSTWANPLSDTLALDAMRLISENLRAAVANGNNLRARQQMA
                     LAAFEAGQASVNARSGAVHALGHSLSGLFDIPERIGDAILLPHVMRANMNVDIQRMAR
                     VAEALGESVAGLSVRAAAQRAIDAVMLLMVDVGLPTSLEKVGADRTAISTLSEQALQD
                     PFLRTNPRALKQKDIEAIYEDAFVEYTEIGIESAPSQGPRATVH"
     gene            complement(1653..1820)
                     /locus_tag="AC16BC_07290"
     CDS             complement(1653..1820)
                     /locus_tag="AC16BC_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241461.1"
                     /translation="MDAVWEDAAQQGTVATIRDLLQRGADIDARDRYGQTARMPATAR
                     WWRPSSHAGPT"
     gene            complement(1846..2160)
                     /locus_tag="AC16BC_07295"
     CDS             complement(1846..2160)
                     /locus_tag="AC16BC_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241462.1"
                     /translation="MLGGRREPWDSSAYWTVSYPVAIALAGVLGYVFPEKPWRWALVL
                     MFTQIAVMIVGGSGFGLLPLGVILLAVLSLPGVALARWAAGIRLRRETPGSSASRSEI
                     SK"
     gene            complement(2229..2783)
                     /locus_tag="AC16BC_07300"
     CDS             complement(2229..2783)
                     /locus_tag="AC16BC_07300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR3241463.1"
                     /translation="MPKLIGISGSLRKGSYNSALLRAAVELAPAGTKVEIGSIAGIPL
                     YDGDLESEHGIPASVAALKDKIAAADGLLLVTPEYNGLLPGVFKNAIDWLSRPASDID
                     RVFGNKPVAIMGATPGRAGTRLAQSAWLPVLRALGARAWFGEQLYVAGAGRVFDAQGR
                     LVDETIKKLLSEFMAGFAAFVNAK"
     gene            complement(2936..3199)
                     /locus_tag="AC16BC_07305"
     CDS             complement(2936..3199)
                     /locus_tag="AC16BC_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241464.1"
                     /translation="MATDDKLCARCGKPVTVNAGDYELFERMHWLCFHLEFEHQGDPD
                     APCSDPSCPWWQIEVLRDRLREMGDDPQVVLERGIAKRHGNDG"
     gene            complement(3575..>4089)
                     /locus_tag="AC16BC_07310"
     CDS             complement(3575..>4089)
                     /locus_tag="AC16BC_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241465.1"
                     /translation="GNTGWLGLAQIWVGGGHITQGLTKLNDTYYNTPTYNTAGWRRLV
                     MCQEIGHTFGLDHQDEGFNNQNLGTCMDYTNNPSGPPSNEHPNQHDYDQLGIIYQHLD
                     GTNTVGQSVNRMPPAAHDTGRGEQDEVGTAQWGQLIRSTNRGRTELYELDLGGGHKMF
                     TFVIWAEGDE"
BASE COUNT          762 a         1356 c         1279 g          692 t
ORIGIN      
        1 tgagcgagat ctcaaacgcg atggcgccac cacggagcac cggggactac caacggctcg
       61 ccgaagcggc tgatgccgcg gcccggtctc cctggaaaac gcgaggattc cttgagttcg
      121 gcgccgccgc actgctcgga gccatcgccg ccatggcgtg ggtcgcctta tcgacaaaag
      181 cggatttccc ccccgcgcgc gctcgggaca tccggccgat cgccgggagg ccgtctcacc
      241 ttccccgatg gggagggaag gtcgccaggg cccggtcagc cacactgtcc atctggcctc
      301 gcgaggtcat catcggcacc cattgtacac gggacattgg gccccacgcc gcccgcgaga
      361 acgccacgca cgtcacgatc ctgacggatc agggtgtcag ccggaaaggg ctcgtcgatc
      421 ccgtgaaggc ctcgcttacc gaggccgggg tgatgtgcga agtcatggat caggtctcgc
      481 gcgaggtacc gcacaccgtg atcgccgaga tcgccggccg gtgcaaggag gcggggacgg
      541 atctgctcat cgcggtcgga ggcgggagcg tgatcgacac cgcgaaggca gtcggaatcg
      601 ttctcaccaa cggcggctcg atccgagact acgagggtgt cgaccgcgtg cgtcgtccga
      661 tcgcgccgct ctatgcggtt ccaaccacgg cgggctgcgg ggccgaaacc agcccattct
      721 gtatcgtgcg ggacgcgacg aagaaacgga agatcgaaat tttcagccgc aagctgatcc
      781 ccgaacggat cttcatcgat ccattgatga cccgcttgat gccgccggat ctgaccgcga
      841 gcagcgggat ggacgccctg gccaacgccg ttgaggcgta ttgttccacg tgggcgaatc
      901 ccctgagcga cacgctcgcg ctggacgcga tgcggctgat ctcggagaac ctccgcgcgg
      961 ccgtggcgaa cggcaacaac ctgagagccc gccaacagat ggcgctcgcg gccttcgagg
     1021 ccgggcaggc ctcggtcaac gcccggagcg gcgcggtgca tgccctcggg cactcgctgt
     1081 cggggctgtt cgatatcccg gaacggatcg gcgacgcgat tctgctgccc cacgtgatga
     1141 gggccaacat gaacgtcgat atacaacgca tggccagggt ggccgaggcg ctgggcgagt
     1201 ccgtggccgg cctgagcgtg cgcgcggcgg cccagcgggc gatcgacgcc gtgatgttgc
     1261 tcatggttga cgtggggctt cccaccagcc tggagaaagt gggcgcggac aggaccgcga
     1321 tctcgaccct cagcgaacag gcgttacaag acccgtttct gcgcaccaac ccccgggcgc
     1381 tcaagcaaaa ggacatcgaa gcgatctacg aggacgcgtt cgtggaatac actgagatcg
     1441 gaatcgagtc tgcgccgtct caaggaccac gcgctacggt ccactgagca ccgaccattc
     1501 agcggctgcc cctgaagggg cagccgctga ggttcgagaa ggttccggag ggcgcaccga
     1561 aaagggggct gtcccctccg gctgcgcgac gtccggatgt ccgaccgcga tcgccagcat
     1621 caactcgctc aacccgaact tggccgtcac attcaggtcg gcccggcgtg cgatgagggt
     1681 ctccaccacc tggcggtggc cggcatgcgc gcggtttgtc cgtaacgatc gcgggcatcg
     1741 atgtccgcgc cgcgctgcaa cagatcgcga atggtcgcaa cggtgccctg ctgggccgcg
     1801 tcttcccaga cggcatccat tcccgcctaa tccggctttc gatggttatt tactgatctc
     1861 ggaacgcgac gccgacgaac cgggcgtctc ccgacgcaaa cggattccgg ccgcccaccg
     1921 cgcgagcgcg acgcccggca acgacagcac ggcgagcagg atcaccccca acggcagcag
     1981 cccgaaaccg gagccgccga caatcatcac cgcgatctgc gtgaacatga ggaccaatgc
     2041 ccagcgccac ggtttctccg ggaacacata cccgagcaca ccggcgagcg cgatcgcgac
     2101 cggataagaa accgtccaat aggcggagga atcccagggc tcgcgcctcc cgccgagcat
     2161 cgacgtcgcg aaccagagaa ccagacccgc ggcggccgca atgccttagc cgagcggggt
     2221 cccgcggctc acttggcgtt cacgaacgcg gcaaagcccg ccatgaactc ggacaggagt
     2281 ttcttgattg tctcatcgac gagccggccc tgggcgtcga acacccggcc cgcacccgcg
     2341 acgtagagct gctcgccgaa ccacgcgcgc gcgccgagcg cccggagcac gggcagccag
     2401 gcgctctgcg ccaaccgcgt gccggcgcgt ccgggagtcg cgcccatgat cgccacgggc
     2461 ttgttgccga acacccggtc aatgtcagac gcgggccgcg acagccaatc gatcgcgttc
     2521 ttgaacacgc cgggcaacaa gccgttgtac tcgggcgtca ccagcagaag accatccgcc
     2581 gcggcgatct tgtccttgag cgccgcgaca gaagccggga taccgtgctc gctctccagg
     2641 tccccgtcgt acagcggaat gcccgcgatg gacccgatct cgaccttggt ccccgccggg
     2701 gccaattcca cggcggcacg aaggagcgcc gaattgtagg aacccttgcg caaactaccg
     2761 ctgatgccga tgagttttgg catggtgtag catctcaatc ccattcatct ctagcacatt
     2821 caggcgaccc gcgtcatggc cacgccagat ccaggatcat cgccgcgctg caatcggata
     2881 aggtgtctgg ccactgacac ctttttcagc cctcttctgg tccgccgacg aggggctagc
     2941 cgtcattgcc gtgccgtttg gcgatgcctc tttccaggac aacctgggga tcgtcgccca
     3001 tctctctcaa ccggtcgcgc aacacctcga tttgccacca gggacatgag ggatcggaac
     3061 aaggcgcatc ggggtcaccc tgatgctcga actccaggtg aaaacagagc caatgcatcc
     3121 gctcaaagag ctcgtagtcc ccggcgttga cggtcaccgg ttttccgcac cgggcgcaga
     3181 gtttgtcgtc cgtggccatc gcgcgccgga gagatcctta cagagccttc cgcctgtgga
     3241 tggtcaccga gttgccgtcg gggtccgaga ccacggccat gtggcacacg ggtgattcca
     3301 tgggttcgac ggcgtgtccc catttctgtc tcgcgaattt caggagaaac gcacgctgat
     3361 gacaccggtg cgtgatacgc cagatgtgat taggaagaaa atagcgatgg gcacgcggca
     3421 tcacgcttcc tccgtgaagc gtgatttggc ccctcaaacg tgcgctcgaa gacccccagc
     3481 ggcctcattg tgaccaacgg ctctgacttt tcaacacact gcacaggtcc gcccccggac
     3541 caagggctct gggatcagcc cccaagagcc atcctcattc gtccccttcg gcccagatca
     3601 cgaaggtgaa catcttgtgg cctccgccga gatcgagctc gtacagctcg gtgcggccgc
     3661 ggttggtcga tcggatcagc tggccccact gagccgtacc cacctcgtcc tgctcgccgc
     3721 gtccggtgtc gtgcgccgca ggtggcatcc tgttgacgct ctggccaacg gtgttcgtgc
     3781 catcaagatg ctggtagatg atgcccagct ggtcgtagtc atgctggttc gggtgctcgt
     3841 tggagggcgg cccggagggg ttgttcgtgt agtccatgca tgtgcccaag ttctggttat
     3901 tgaaaccctc atcctgatga tcgagaccga aggtgtggcc gatctcctga cacatcacga
     3961 gccgtctcca tcccgcagtg ttgtacgtcg gggtattgta gtacgtgtca ttgagcttgg
     4021 tgaggccctg cgtgatgtgg ccacctccga cccaaatctg agccaggccg agccagccgg
     4081 tgttgccga
//
LOCUS       DBNGTT010000324         2909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2627951, whole genome shotgun sequence.
ACCESSION   DBNGTT010000324 DBNGTT010000000
VERSION     DBNGTT010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2627951"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="AC16BC_07315"
     CDS             complement(<1..1244)
                     /locus_tag="AC16BC_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR3241466.1"
                     /translation="MDKRKTDETLREFLAESDELLDALHRHLELIEQTPDSITPETLN
                     ALFRAAHTLKGMSGVMGLVSVSELAHSLEDVMDRLRMGKVTMSRPLRNVLTDGVEALG
                     HLIASAASGQAPSDITGLVTRLRDAMSAPQAAGTPTRAVGVDSEILKVLTEYEQHRLQ
                     ENIKARRNLFEVRAHFRFEDFDKSLSRMTEKLQTVGEVITTLPSSGGSMDTGIAFTLL
                     VGAGHDREALAQLVADDGAEVVLIGGRESAAPKTPSAPDQDSALSRSLSQTIRVDIAK
                     LDGLLNMLGELVLSKSVIVQIARDLLQNGGLVGPAMELHKAAQALEHRVSGLQEGLIE
                     VRMIPVAQLFDRSMRAIKKIARDLGKEIQVTTSGEETKLDKSMVEAIADPLLHLIRNA
                     LDHGIESREDRHRAGKPAIGTI"
     gene            complement(1247..1633)
                     /locus_tag="AC16BC_07320"
     CDS             complement(1247..1633)
                     /locus_tag="AC16BC_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3241467.1"
                     /translation="MSKPTNMKRVLVVDDSPTTRALIVSAIEEMEGFDTIEAPNGFEA
                     LKRIPIQPLDLIVTDINMPDINGLEIVHFVKHHPDFRAIPLIIVSTEGSDEDVRRGLN
                     LGASAYVKKPFDPEHLQATIRRLVAT"
     gene            complement(1656..2852)
                     /locus_tag="AC16BC_07325"
     CDS             complement(1656..2852)
                     /locus_tag="AC16BC_07325"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR3241468.1"
                     /translation="MSLEGRLEDLGLPDIFQIINLSKRSGGLTLIKKDGMGRVVFNQG
                     NVVFASSDNKSRFGYALLQRDLLSRADLERALGVQKARTVKKPIGTILVEMGVMDKER
                     LEAELRLHIIDVVRDLLTWQTGTFQFTLGKFSEEGVLMCSGLSAEFLLLEGARLDDES
                     GGDDGADATMLDRESGEGSAGFLDESTPMSHPVTAGQDHESLSGLVNDEDLGTAIIVD
                     RSRASRKDLELLPAMIEELSGAGSGHDVFLMMLRFAGELMNRAVIFLVRGDAVTGWGQ
                     SGVEVDEGSADEMVRSILVPLSDASSFRDVADTRVSLKGPWPDQPGNQLVVERLGGVW
                     PPEGFLTPIVSGDQVVAFLYGDNLPNHSPIGPTEGLEAFIRVAAVALGKTVLQQQMNR
                     TRTGGS"
BASE COUNT          551 a          941 c          837 g          580 t
ORIGIN      
        1 tgaatggtgc cgatcgcggg tttgccggcc cggtgccggt cctcccgcga ctcgatcccg
       61 tgatccaggg cgttgcggat caaatgcagg agcgggtccg cgatcgcctc aaccatcgat
      121 ttatcaagct tggtttcttc gcccgaggtc gtgacctgaa tttccttccc gagatcgcgc
      181 gcgatcttct tgatcgcccg catcgagcga tcgaagagtt gcgccaccgg aatcatccgg
      241 acctcgatga gcccttcctg caaacccgac acgcgatgtt caagggcctg cgccgcttta
      301 tgaagctcca tcgccggccc cacgagtccg ccgttctgga gcaaatccct ggcaatctga
      361 acgatgacgg atttcgacag gaccagttca ccgagcatgt tcaacaagcc gtcaagcttc
      421 gcaatatcca cgcgaatcgt ctgactgagg ctgcgggaca acgcggagtc ctgatccggc
      481 gcactcggag ttttcggcgc cgccgattcc cgcccgccga tgagcacgac ttccgcgccg
      541 tcatcggcga ccagttgcgc aagcgcctca cggtcgtgcc cggcccccac caataacgta
      601 aaggcgattc cggtatccat ggagcccccg gaactcggca gcgtggtaat cacttccccc
      661 acggtctgga gcttctcggt catccgagac agcgacttgt cgaaatcctc aaaacgaaaa
      721 tgggcgcgga cttcgaacag attgcgtcgc gccttgatgt tctcctgaag ccgatgttgt
      781 tcgtactcgg taagcacctt caagatttcc gaatcgacgc cgaccgccct ggtcggagtt
      841 ccggcggctt gcggggcaga catcgcgtcg cgcagacgcg tgaccaggcc ggtgatgtcg
      901 gacggtgcct gtcccgacgc cgccgacgcg attaaatgtc ccaaggcctc cacgccgtcg
      961 gtcaagacat tgcggagcgg acgactcatc gtcaccttcc ccatgcgaag ccgatccatc
     1021 acgtcttcga gcgagtgagc cagttctgac accgaaacca agcccatgac gccggacatg
     1081 cccttaagcg tgtgggccgc acgaaacagc gcgttgagcg tttccggggt gattgagtcg
     1141 ggcgtctgtt cgatcagctc cagatggcgg tgcagggcgt cgaggagttc gtcggactca
     1201 gccaagaact cccgcaacgt ctcgtccgtt tttcgtttgt ccatcgctag gtcgcgacca
     1261 gccgccggat ggtcgcctga aggtgttcgg ggtcgaacgg tttcttcacg taggccgatg
     1321 cgccgaggtt gaggcctctc ctcacatcct cgtcgctgcc ttcggtcgag acgatgatca
     1381 acgggatcgc ccggaagtct gggtgatgct tcacgaagtg cacgatctcc agcccgttga
     1441 tgtcgggcat attgatgtcc gtcacgatga ggtcgagcgg ttgtatcgga atgcgcttga
     1501 gcgcctcgaa tccgttcggc gcctcgatcg tgtcgaagcc ctccatctcc tcgatggccg
     1561 aaacaatcaa ggcccgcgtc gtgggcgagt cgtccacaac caagacgcgc ttcatgttcg
     1621 tcggtttgga cacgcgggtg agtatagcac agcggtcatg aaccgcctgt ccgtgttcgg
     1681 ttcatttgct gttggaggac cgttttcccg agcgcaaccg ccgcgacgcg aatgaacgcc
     1741 tcaagacctt cggttggtcc gatcggcgaa tggttcggaa gattgtcgcc gtagaggaac
     1801 gccacgactt gatcgccgct cacgatcggc gtcaggaacc cctccggagg ccacactccg
     1861 cccagccgtt ccaccaccaa ttggtttccc ggttgatcgg gccacggccc cttgagcgac
     1921 acccgggtat cggccacgtc gcgaaacgac gaggcgtcgg acagcgggac caggatcgat
     1981 cggaccatct catcggccga cccctcgtcc acctcgacgc cggactgtcc ccaccccgtc
     2041 accgcatcgc ctcgcacaag aaaaatgacc gcccggttca tcagttcgcc ggcaaaacgc
     2101 agcatcatca ggaacacgtc gtgcccagac cctgcgccgg ataattcttc gatcatcgcc
     2161 ggcaacaatt ccaggtcctt gcgcgacgcg cgggaccggt cgacgattat ggccgtcccc
     2221 aagtcctcgt cgttgacgag ccccgaaagg ctttcgtgat cctgtcccgc cgtcacaggg
     2281 tgcgacatgg gcgtggactc atccaggaat ccagcggacc cctcgccgga ttcgcgatcg
     2341 agcatcgtgg cgtcggctcc gtcatcgccg cccgattcgt cgtcgaggcg agcgccttcg
     2401 aggagcagaa actcggcact cagtcccgaa cacatcaaca ccccctcctc ggaaaacttg
     2461 ccgagcgtga actggaacgt gccggtttgc cacgtcaata gatcccggac cacgtcaatg
     2521 atgtgcagtc tcaattccgc ttcgagccgt tctttgtcca tcacccccat ctccaccaga
     2581 atcgtcccga tcggtttctt gaccgtacgg gccttctgga cgccaagggc gcgttcgaga
     2641 tcagcgcggg acaagagatc gcgctgcagg agggcgtacc caaatcggct cttgttgtcg
     2701 gagctggcga agaccacgtt gccttggttg aagaccacgc ggcccatccc gtctttcttg
     2761 atgagggtga gaccgcccga gcgtttgctc aggttaatga tttggaaaat atccggtagg
     2821 ccaaggtctt cgagacgacc ctcaaggctc atagcctctg gggtgtgcgg tgttcagcgg
     2881 tgtcggcgcc gtccagcacc tcataacgg
//
LOCUS       DBNGTT010000325         3142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_584178, whole genome shotgun sequence.
ACCESSION   DBNGTT010000325 DBNGTT010000000
VERSION     DBNGTT010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_584178"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /gene="amrS"
                     /locus_tag="AC16BC_07330"
     CDS             complement(<1..911)
                     /gene="amrS"
                     /locus_tag="AC16BC_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR3241469.1"
                     /translation="MRNREDRSGYAKGDRPGAAVVPTRFWHRLDDGRVQCDLCPRFCK
                     LHEGQQGLCFVRARRSDQIVLTTYGRSSGFCVDPIEKKPLNHFLPGTPVLSFGTAGCN
                     LSCKFCQKWDISKSRETDTLADEASPDAIVRAARSLGCRSVAMTYNDPVIFHEYAVDV
                     AKACRAQGIMAVAVTAGYVCDEPRREFYQHMDAANVDLKAFTERFYQDVTASHLQPVL
                     DTLIYIKRHTSVWLEITTLLIPGENDSDEELNALTRWVVDELGQDVPLHFSAFHPDYK
                     MLDRPPTPPATLTRARRIAMNNGLRYV"
     gene            complement(901..1344)
                     /locus_tag="AC16BC_07335"
     CDS             complement(901..1344)
                     /locus_tag="AC16BC_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241470.1"
                     /translation="MKGLSVVAVAMAAAVIGSCQASESIRRVTYPPDFRYLEQHDVTS
                     AMWRLARDVRALKALLGGATDLPPQSHQEVIRLLRDMEATASEIDPKGRRSTHPRIDS
                     NIGAFRRDLAMARQAAEREPPNYFLAGNVAGSCSYCHEGQREDAE"
     gene            complement(1395..2327)
                     /locus_tag="AC16BC_07340"
     CDS             complement(1395..2327)
                     /locus_tag="AC16BC_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR3241471.1"
                     /translation="MTPLILSLGPPEMADAVAKGIAGDEAGAVIRRFPDGETYVRLDT
                     DVRGRQVVIVADLAAPDEKIVPLLWTAATARDLGAARIGLAAPYLPYMRQDARFQPGE
                     GVTSRYFAGVLSSHVDWLVTVDPHLHRYRSLDEVYAIPSRVRSAAPLLARWIDRNVRA
                     PLVVGPDAESEQWVRVVANAVGAPCVVLTKTRVGDREVRVSPPDVAPWRERTPVLIDD
                     VISTGGTMVETVRAVLKAGMAGPVCLAVHGIFVGGAHDELMAAGCRRVVTCNTVPHPT
                     NGVDAAPLLVEGIREMMDRNVAANPPVPPVVKGG"
     gene            complement(2324..>3142)
                     /locus_tag="AC16BC_07345"
     CDS             complement(2324..>3142)
                     /locus_tag="AC16BC_07345"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121540.1"
                     /note="catalyzes the formation of thymine and
                     2-deoxy-alpha-D-ribose 1-phosphate from thymidine; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR3241472.1"
                     /translation="KVVAKEGGCLVWGGAVLLSPADDVLIRVSRPLDLDGEGQLVASI
                     LSKKAAAGSTHVVVDLPVGPTAKVRTAEGAYGLSERLTQVGRAVGLEVRVVITDGSQP
                     VGRGLGPALEARDAVAVLRREPHAPSLLRGRALALAGYVLELAGKAERGSGYTMARAA
                     LDDGRAWRKFEAICDAQGGMREPPRSSHTSVVTSKRVGRVAEIDNRRLARIAKLSGAP
                     QAPAAGIELHTSLGAAVELGQPLLTIHAEAPGELAYAVGYAQSQADVIRIEEPS"
BASE COUNT          506 a         1136 c         1000 g          500 t
ORIGIN      
        1 tacacgtacc gcagaccgtt gttcatggcg atccggcgcg cccgcgtgag cgtggcgggc
       61 ggggtcggcg ggcgatcgag catcttgtag tcggggtgaa acgcggagaa gtgcaggggc
      121 acgtcctggc ccaactcgtc aacgacccag cgcgtaagcg cgttcagttc ttcatcggag
      181 tcgttttcgc ccggaatcag gagcgtggtg atctccagcc acaccgacgt gtggcgtttg
      241 atgtagatga gagtgtccag cacgggctgc aaatgggagg cggtcacgtc ctgatagaac
      301 cgctcggtaa acgccttcaa atccacgttg gccgcatcca tgtgttgata gaactcgcgc
      361 cgcggctcgt cgcagacata gccggcggtg accgccacgg ccatgatccc ctgcgcgcgg
      421 cacgccttcg ccacgtcaac ggcgtactcg tggaagatca cggggtcatt gtaggtcatg
      481 gccacgctgc gacaaccgag gctccgtgcc gcgcggacga tcgcatcggg cgacgcttcg
      541 tccgccaagg tgtcggtctc acgggacttg ctgatgtccc atttctgaca gaacttgcac
      601 gacaggttgc accccgcggt cccgaacgag agcacgggcg tgcccggcag aaagtggttg
      661 agcggtttct tttcgatggg atccacgcaa aagccgctgg agcggccgta cgtggtcagg
      721 acgatctgat cgctccgccg agcgcgaaca aagcagagcc cctgctgccc ctcgtgcagc
      781 ttgcagaacc tcgggcacag atcgcattgc acccgcccat cgtcgagtcg gtgccagaaa
      841 cgggtcggca caacggcagc gcctgggcgg tcacccttgg cataccctga gcgatcctcc
      901 ctattccgca tcctcgcgtt gtccctcgtg gcaatagctg cacgagcccg ccacgttgcc
      961 ggcgagaaaa tagttcgggg gttcgcgctc cgcggcctgg cgcgccatgg ctaggtcacg
     1021 gcgaaacgcc ccgatgttgc tgtcgatccg cgggtgcgtg ctgcgacggc ccttggggtc
     1081 gatctcgctc gcggtcgctt ccatgtcgcg aaggaggcga atcacttcct ggtgactctg
     1141 aggcggcaag tccgttgcgc cgccgagcag cgccttgagc gcgcgcacgt cccgcgcaag
     1201 ccgccacatc gcggacgtga catcgtgctg ctccaggtac cggaagtccg gcgggtacgt
     1261 cacgcgccgg atggactcac tggcctgaca actcccgatc actgcggccg ccattgcgac
     1321 cgccacgaca gaaagtcctt tcacgttcca tcctccagga tcacaccggt ctcgttccgt
     1381 gccccagcct cttcctaccc ccctttgaca acgggtggta cggggggatt tgccgcgacg
     1441 tttcgatcca tcatctcccg aatgccttcg accagcaacg gcgcggcatc cacgccgttg
     1501 gtcgggtgcg gcaccgtgtt gcaggtgact acccggcggc agccggccgc catcaactcg
     1561 tcgtgggcgc cgccgacgaa aattccgtgc accgcgaggc acacggggcc ggccatcccg
     1621 gccttgagca cggcgcggac cgtctcgacc atggtgcctc cggtggagat cacgtcatcg
     1681 atgagcaccg gcgttcgctc gcgccacggg gcgacgtcgg gcggcgagac tcgcacctcg
     1741 cggtcgccga cccgcgtctt ggtcagcacc acgcacggcg cccccaccgc gttcgccacc
     1801 acccggaccc actgctcgct ttcggcgtcg ggaccgacca ccagcggcgc ccgcacgttg
     1861 cggtcgatcc accgggccag gagcggcgcc gcgctccgga cgcggctcgg aatcgcgtac
     1921 acctcgtcga gcgagcggta ccggtgcaga tggggatcca cggtcacgag ccaatccacg
     1981 tgcgacgaca gcacgccggc aaaatagcgc gacgtgacgc cttccccggg ctgaaaccgc
     2041 gcgtcctgtc gcatgtaggg caggtacggc gccgcgaggc cgatgcgcgc ggcgccaagg
     2101 tcacgggccg tcgccgcggt ccacaggagc gggacgatct tctcgtccgg cgccgcaaga
     2161 tccgccacga tgaccacctg ccgaccccgc acgtcggtgt ccagtcggac gtaggtttcc
     2221 ccgtccggaa accgtcggat cacggctccc gcctcgtcgc ccgcaatgcc tttcgccacg
     2281 gcgtcggcca tttcgggcgg gccgagcgag aggatcagcg gcgtcacgac ggttcctcga
     2341 tgcgaatcac atcggcctgc gattgggcgt atcccacggc gtaggccagt tcgcccggag
     2401 cctcggcgtg gatggtcaac aagggctgac cgagttccac cgcggcgccg agggaggtgt
     2461 gcaactcgat gccggccgcc ggcgcctggg gtgcgcccga cagcttggcg atgcgcgcga
     2521 ggcgccgatt gtcgatctcc gccacccgac ccacccgctt cgacgtcacg accgaggtgt
     2581 ggctcgaccg cgggggctcg cgcatgccgc cctgggcgtc gcagatcgcc tcgaacttgc
     2641 gccacgcgcg gccgtcgtcc agcgcggcgc gcgccatcgt ataccccgat ccccgttcgg
     2701 ccttgcccgc caattccagc acgtaccccg ccagcgcgag cgcgcgcccc cgcaacaatg
     2761 acggcgcgtg gggttcgcgg cgcaacaccg ccacggcgtc gcgcgcttcg agcgcgggcc
     2821 ccagaccgcg ccccaccggc tgtgagccgt cggtgatcac gacccgcacc tccaacccca
     2881 cggcgcgtcc cacctgcgtc aaacgttcgc tgagcccgta cgcgccttcc gcggttctca
     2941 ccttggcggt ggggcccacg ggcaaatcca ccaccacgtg ggtggacccc gcggccgctt
     3001 tcttcgacag gatcgacgcc accaactggc cctcgccgtc caaatccagc ggccgcgaca
     3061 cgcggatcag cacgtcgtcg gccgggctca gcaacacggc ccctccccac accagacagc
     3121 cgccttcctt cgccacgacc tt
//
LOCUS       DBNGTT010000326         3523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_713177, whole genome shotgun sequence.
ACCESSION   DBNGTT010000326 DBNGTT010000000
VERSION     DBNGTT010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_713177"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC16BC_07350"
     CDS             complement(<1..310)
                     /locus_tag="AC16BC_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HIR3241473.1"
                     /translation="MTTPFVVVLGANGVFGRRTLHSLVRRSGLRIGAACRTKEQATAL
                     VRSFGGAVRPLWGDVTRMDDLRACLDGAQAVFHCAGSFDTQPLLPLSVALERGLDYAD
                     L"
     gene            complement(341..826)
                     /locus_tag="AC16BC_07355"
     CDS             complement(341..826)
                     /locus_tag="AC16BC_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3241474.1"
                     /translation="MRKVNTRDIAEEAWAPPKGKFGGASREISVALGRNPQSTDLKER
                     HPFDVEICRIPPGKIHPPYHSHSAQWEFYHVMSGAGPVRHQEGTTPIESGDAFLFEPG
                     QPHQLINNGSSDLVLYVVADNPIGESCHYPDTGKWLVRSPEHRVLRFDGVEHDYYDGE
                     E"
     gene            complement(879..1199)
                     /locus_tag="AC16BC_07360"
     CDS             complement(879..1199)
                     /locus_tag="AC16BC_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3241475.1"
                     /translation="MKIHGIFRIGGGLALVAWAAVASAADLEAGKKLYDQLRCARCHA
                     INDQGGKTGPDLSHIGAVRDRKWLIQFLKQPKSVIADAKMMAVRASDAELAALADYLA
                     SLKP"
     gene            complement(1218..2396)
                     /locus_tag="AC16BC_07365"
     CDS             complement(1218..2396)
                     /locus_tag="AC16BC_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241476.1"
                     /translation="MKRWTPRIISVAFCSALGLVWGWQPPDAEAIPAWARQYSADCSL
                     CHYPAVPRLNATGYRFRWAGFRMPEEFGQEPDVTAVGNYLSARGRGRYVYADSDQTET
                     VSEFQWHDTTLFYAGAITPHLSAFNELEWEAEDEVSLVAQLQWLSGRSEHFTTVRAGQ
                     FHTLPRVGIGPLDRPTGISTPSIFSERLTTSPIPFRLSDDQRGIEVAHIVRTSRLALQ
                     ATNGLDASGSGTAGDADTAKDVLLVYERLLDATASGLTLLGYRGVWHDGLIDDDYTFY
                     RYGGTVNKVLPVGLDVLGGYIRSHDHLPSVVGPNVQAQAFFLELQQYLRRPDVTVLAR
                     YDWIDPDQDTAQDRRQRQTLGVVKSVERYLRLALEGQRATDRASATGYALTAEAMVNF
                     "
     gene            complement(2420..3481)
                     /locus_tag="AC16BC_07370"
     CDS             complement(2420..3481)
                     /locus_tag="AC16BC_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3241477.1"
                     /translation="MERMNEYRTLGRTGLRVSPLGLGVMTYGWGADKQAARAMFDLYR
                     ERGGNFFDTADVYTGGQSETWLGEFVHEARVRDEVVIATKFSFNAQAGNPNAGGNGRK
                     NIYRALEGSFRRLKTDYVDLYILHAWDRVTPVEEVMSTLNDLVRAGKVRHIGLSDAPA
                     WYAARAQTLAEAYRYERLALLQLEYSLVSRSLERKHLPLAKEMGISLVPWSPLGSGFL
                     TGKYKRVDGKITGSGRVFDTKDSGNPVMEKFAKREKSWEILDALLKAAKELGKTPTEV
                     ALAWVLGRPQVTSVLVGATKREQLELNLGALAIELPAEIERRLTEVSRPESTELDHFF
                     EPTMQGMIHGGVRVRRGVP"
BASE COUNT          615 a         1213 c         1081 g          614 t
ORIGIN      
        1 ccaagtcggc ataatccaga ccgcgttcga gcgcgaccga cagcggcagc aacggttgcg
       61 tatcgaacga ccccgcgcaa tgaaacaccg cctgggcccc gtccaggcac gcgcgcaggt
      121 cgtccatacg cgtcacatcg ccccacaaag gccggaccgc tccgccgaac gagcggacca
      181 acgccgtggc ctgctccttg gtccggcacg ccgcgccgat ccggagaccc gaacgacgaa
      241 ccaaggagtg aagggtccgg cgcccaaaga caccattggc gcccaggaca accacgaatg
      301 gggtggtcac gttagactct tgatccgact gcagatccgt tcattcctcc ccgtcataat
      361 agtcgtgctc cacgccatcg aagcggagca cccggtgctc gggggagcgc accagccatt
      421 tgccggtgtc gggatagtga caagactcgc cgatggggtt atcggccacg acgtagagca
      481 ccaggtccga ggagccgttg ttgatgagct ggtgcggctg accgggttcg aacagaaacg
      541 cgtcgcccga ttcaatgggc gtggtcccct cctggtgccg gaccgggccc gcgcctgaca
      601 tgacgtggta aaactcccac tgcgcgctgt gcgagtgata cggcgggtgg atcttgcctg
      661 gagggatgcg gcagatctcc acgtcgaacg gatgccgctc cttcaggtcc gtggactgcg
      721 ggttccgtcc cagcgcgacc gagatctccc tgctcgcccc gccgaacttc cccttgggcg
      781 gcgcccacgc ctcctcggcg atgtccctgg tattcacttt gcgcatcttc cttactccaa
      841 cggttcagca attggggaac tgcccaaggt ggagcggctc atggcttgag cgaagcgagg
      901 tagtcggcta gggccgccag ctcggcatcg ctcgcccgca ccgccatcat cttggcgtcg
      961 gcgataaccg acttcggctg tttcagaaac tggatcagcc acttgcggtc gcgcacggca
     1021 ccgatgtgcg ataaatcggg gccggtcttt ccaccttggt cgttaatggc atggcagcgc
     1081 gcgcagcgga gttggtcata cagcttcttg ccggcctcaa gatcggccgc cgaggcgacc
     1141 gccgcccagg ccacaagcgc caggccgccc ccgatccgaa agatcccgtg gatcttcacc
     1201 gtaccgtcct cgttgcgcta gaagttgacc atcgcctcgg ccgtcagcgc gtagccggtg
     1261 gccgaagcgc gatccgtggc ccgctggcct tcgagcgcga gccgcaggta gcgctcgacc
     1321 gacttgacca ccccgagcgt ctggcgctgg cgccggtcct gcgcggtgtc ctgatccggg
     1381 tcgatccaat cataccgagc gagcactgtg acatcgggcc ggcgcagata ctgctgcagc
     1441 tcgaggaaaa aggcctgggc ctggacgttc ggcccgacga cggagggcag atggtcgtgg
     1501 ctgcggatgt atccgcccag cacgtcgagt ccgaccggaa gcaccttatt gacggtgcca
     1561 ccgtaccggt agaaggtgta gtcatcgtcg atgaggccgt cgtgccacac gccacggtag
     1621 ccgagcagcg tgaggccgga tgcggtcgca tccaggagac gctcgtagac gagcaagaca
     1681 tccttagcgg tatctgcatc gcccgcggtc ccggagccgc tcgcgtcgag cccgttcgtc
     1741 gcctgcagcg ccaggcgaga ggttcgtacg atatgcgcca cctcgatgcc gcgttggtcg
     1801 tccgaaagcc gaaacgggat agggcttgta gtcagccgct cggagaagat cgacggcgtg
     1861 ctgatcccgg tcggccgatc caatggcccg atcccgaccc gcggcaacgt atggaattgg
     1921 cccgcgcgaa ccgtcgtgaa gtgctccgaa cgccccgaga gccactggag ctgggccacc
     1981 aacgacacct cgtcctcggc ctcccattcc aactcattga aggccgagag atgcggtgtg
     2041 atcgccccgg cgtagaacag cgtggtatcg tgccattgaa attcgctcac cgtctcggtc
     2101 tggtccgagt cggcgtacac gtaacggccg cggccccggg ccgagagata gttcccgacc
     2161 gccgtcacgt ccggttcctg gccgaattct tcgggcatgc gaaaaccagc ccaccggaag
     2221 cgatagcccg tcgcgttgag tctcgggacg gccggatagt gacacaggct gcagtcggcg
     2281 ctgtactgcc gcgcccaggc cggaatcgcc tcggcgtccg gcggctgcca tccccatacc
     2341 aacccaagcg ccgaacagaa cgcaacactt atgatccggg gcgtccatcg tttcatcgtc
     2401 gcctccttcc gtctcagggt caggggacgc cgcgtctcac gcgcacgccg ccgtggatca
     2461 tgccctgcat ggtgggttcg aagaagtgat cgagttcggt ggactcgggc cggcttacct
     2521 cggtgagccg gcgctcgatc tcggccggca gctcgatcgc aagcgccccg agattgagct
     2581 ccaactgctc ccgtttcgtg gccccgacca gcacgctcgt cacctgcggc cgtcccagca
     2641 cccacgcaag cgcgacctcg gtcggcgtct tgccgagttc cttggccgcc ttgagcaatg
     2701 cgtcgaggat ctcccagctt ttttcgcgct tggcgaattt ctccatcacg ggattgcccg
     2761 agtccttggt gtcgaacacc cgacccgagc ccgtgatctt cccgtccacg cgtttgtact
     2821 tgccggtcag gaagccgctg ccgagcgggc tccacggcac gagcgaaatg cccatctcct
     2881 tggccagcgg caggtgcttc cgctcaagac tgcgcgagac caacgagtat tcaagttgca
     2941 gcagcgcaag ccgctcgtac cggtacgcct ccgcgagcgt ctgggcccgc gccgcgtacc
     3001 acgcgggcgc atcgctcaat ccgatgtgcc gcaccttgcc cgcccgcacc aggtcgttga
     3061 gcgtgctcat gacctcctcc accggggtca cgcgatccca ggcatggagg atatagagat
     3121 cgacgtaatc ggtcttcagg cggcggaacg acccctcgag cgcgcggtaa atgttcttgc
     3181 ggccgttgcc gcccgcgttc ggattgcccg cctgggcgtt gaacgagaac ttggtggcaa
     3241 tgaccacctc gtcgcgcacc cgcgcctcgt gcacgaactc gcccagccac gtctcgctct
     3301 ggccgcccgt gtacacgtcc gcggtgtcga agaagttgcc gccgcgttcg cggtacaaat
     3361 cgaacatcgc gcgcgccgcc tgcttgtccg ccccccaccc atacgtcatc accccaagac
     3421 caagcggcga caccctgagc ccggtgcgcc cgagcgtgcg gtactcgttc atgcgttcca
     3481 tagggccctc atcgatagtt catctaccgc aacggccgct cat
//
LOCUS       DBNGTT010000327         4340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1991370, whole genome shotgun sequence.
ACCESSION   DBNGTT010000327 DBNGTT010000000
VERSION     DBNGTT010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1991370"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="AC16BC_07375"
     CDS             complement(<1..1669)
                     /locus_tag="AC16BC_07375"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR3241478.1"
                     /translation="MTPTAVATHIIPPDIEEEIAHYEQEAQRFLRGEIHPEVFRRFRL
                     QHGIYGQRQDNVQMVRIKIPFGGMTVPQLRRVADIGDRYSRGVAHLTTRQDIQLHYIK
                     LEYTAAIMRQLAEVGLTTREACGNTVRNVTACPYAGICRDEVVDVTPYAHGMAMHFLR
                     NPVCENLPRKFKIAFSGCASDCALTGIHDFGVLAVRRVVDGREEIGFRLTVGGGLGPS
                     PKRPYLLEEWVPAAELRPRCEAVLRVFNRHGNRKNRSLARLKFLIEKSGFEKFYAMYA
                     EEYAFVREGRDASALAAFAPMSEPVPSNGSKRPHSNGSRSPRFEAWQATNVKPQKQAG
                     YSAALVTLPIGDLTTAQLRGLADVTERFAHGSIRVTVTQNFLLRWVADADLPALHDAL
                     TAIGLGTPGADRVGDVITCPGADTCGLGITSSKGVGLALSEQLAHNGKAFAEDLKGID
                     IKVSGCPNSCAQHHIASIGFHGLGHRIEGQLIPAYQLHLGGRSTDQGVAFGLLAGKYP
                     AKRLPEVVDALLEYYREHRQAGETFTAFVDRVGKGPIVAALEPYTSLP"
     gene            complement(1694..2440)
                     /locus_tag="AC16BC_07380"
     CDS             complement(1694..2440)
                     /locus_tag="AC16BC_07380"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015165749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HIR3241479.1"
                     /translation="MSTATLYSKDQLQTLNQQYTTQPAEAVLKWALKEFGSQVALACS
                     FGAEDVVLVDMMCKIDRAPRIFCLDTGRLHSETYEVMDAVRDRYGISIETYFPQAPAV
                     ESMVRAKGVNLFYQSIENRKECCGVRKVEPLGRALSGLRAWITGLRSAQAVTRADMPM
                     VEHDAGHGGIAKINPLIHWSESQVWDYIKTNKVPYNKLHDQGFPSIGCAPCTRAIKPG
                     EDVRAGRWWWESPEHKECGLHGGPKATAGR"
     gene            complement(2480..2944)
                     /locus_tag="AC16BC_07385"
     CDS             complement(2480..2944)
                     /locus_tag="AC16BC_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3241480.1"
                     /translation="MLKISAKSEYGMLALLYLTCHSASGPVAVTTMADDLDIPRRFLE
                     QIVAEFKHRGLVESLRGAKGGYRLSKDPTSITMREIFRVTEGSFFQWTEASAANACAD
                     ATPNLRVIRDAWTTVQGSVNDVLGSITLQQMCDRAKQLQSDGPAVAVAGRAV"
     gene            3125..>4340
                     /locus_tag="AC16BC_07390"
     CDS             3125..>4340
                     /locus_tag="AC16BC_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3241481.1"
                     /translation="MPWQSIVAILVLVLANGFFAAAEIAILTVRKSRILHLVEAGKHR
                     AKDVQRLQADPERFLATIQIGMTVISALAGAVGGVTAIENIKPYLEGIPNPVLRNASE
                     GIALTIVVASISYLSLVFGELVPKTLALRYPEQIALWVSWPIDALSRFSSVVVKVLMW
                     SSRLILKPLGARSKPAFFPEEEIKSLLIEGRQTGEIDRTEQELIHSVFEFADISVKEV
                     MVPPPKIHAIKLDTPVETVLADITENKFSRYPVYREELNDICGVVYTKDILDAMVRRR
                     PIDIKSLMHPAYFVPESMKVSHLLQEMQRRHTQMAIVVNEFGTVEGLATMEDLIEEIV
                     GEIRDEYDLEERPVERLKDGSLVVDASLSVRDLREEYGLPIPESPLYETLGGFVMAQL
                     QNIPRSGAITHHN"
BASE COUNT          731 a         1354 c         1415 g          840 t
ORIGIN      
        1 acggcaggct ggtgtacggt tcgagcgcgg ccacgatcgg ccccttgccc acgcgatcca
       61 cgaacgcggt gaacgtttcc ccggcttggc ggtgttcgcg gtaatactcc agtagcgcgt
      121 ccaccacttc cgggagccgt ttggcggggt acttgcccgc caacagcccg aacgccacgc
      181 cttggtcggt cgatcggcca cccagatgca attggtacgc ggggatgagt tggccctcga
      241 tgcgatgccc gagtccgtgg aacccgatcg acgcgatgtg gtgttgggcg caggagttgg
      301 ggcagccgct gaccttgatg tcgatgccct tgaggtcctc ggcgaacgcc ttgccgttgt
      361 gcgccaattg ttcgctgagc gcgagcccca cgcccttgga cgaggtgatg cccagaccgc
      421 aggtgtcggc gccggggcac gtgatcacgt cgcccacgcg gtcggctcca ggcgtgccga
      481 ggccgatcgc ggtgagcgca tcgtgcagcg cgggcaaatc cgcgtccgcc acccagcgca
      541 ggagaaagtt ctgcgtcacg gtcacccgga tgctgccgtg ggcgaaccgc tccgtgacgt
      601 cggccaggcc gcgcaattgc gccgtggtca aatcgccgat cggcagggtc accaatgccg
      661 cggaatagcc ggcttgtttc tgcggcttca cgttggtggc ttgccaggcc tcgaaccgcg
      721 gggagcgcga cccgttcgaa tgcggccgct tggacccgtt gctcggcacc ggctccgaca
      781 tcggcgcgaa cgcggcgagc gccgaggcgt cgcggccttc gcgcacgaag gcgtactcct
      841 cggcatacat ggcgtagaac ttttcaaacc cgctcttttc gatgagaaac ttgagccggg
      901 ccaggctgcg gttcttgcgg ttgccgtggc gattgaacac ccggagcacg gcctcgcagc
      961 gggggcgcaa ttccgcagcc ggcacccact cttccagcag gtagggccgt ttggggctgg
     1021 ggcccaggcc gccgccgacc gtcagacgga acccgatctc ttcgcggccg tccaccacgc
     1081 gtctgaccgc cagcaccccg aagtcgtgga tgccggtgag cgcgcagtcg ctcgcgcagc
     1141 cggaaaacgc gatcttgaac ttgcgcggca agttttcgca caccgggttg cgcagaaagt
     1201 gcatggccat gccgtgggcg tagggcgtca cgtccaccac ttcgtcgcgg cagatccccg
     1261 catacggaca ggcggtgacg ttccggaccg tgttcccgca cgcctcgcga gtcgtcaggc
     1321 ccacttcggc gagttgccgc atgatcgcgg cggtgtattc gagcttgatg tagtgaagct
     1381 ggatgtcctg tcgcgtggtc aggtgggcca cgccgcgcga gtaccggtct ccgatgtcgg
     1441 cgacccggcg cagctggggc acggtcatcc cgccgaacgg gatcttgatg cgcaccatct
     1501 ggacgttgtc ctgccgctgg ccgtagatcc cgtgttgcaa ccgaaatctc cggaacacct
     1561 cggggtggat ctcgccgcgg aggaagcgct gggcttcctg ctcgtagtgg gcgatctcct
     1621 cctcgatgtc cggggggatg atgtgagtcg cgactgccgt cggtgtcatg acgtgctccc
     1681 tttcgcgaac gggtcagcgg ccggccgttg ccttcggccc gccgtgcaac ccgcactctt
     1741 tatgttccgg cgattcccac caccaccggc ccgcgcggac gtcttcgccg ggcttgatcg
     1801 cgcgcgtgca cggcgcgcag ccgatgctgg ggaatccctg atcgtgcaac ttattatagg
     1861 ggaccttgtt ggttttgatg tagtcccaca cctgtgattc cgaccagtgg atcaaggggt
     1921 tgatcttggc gatgccgccg tggccggcgt cgtgctcgac catgggcatg tccgcgcggg
     1981 tgacggcttg tgcgctccgc agccccgtga tccaggcccg cagcccggac agggcccgcc
     2041 ccaggggctc gaccttgcgc acgccgcagc actccttgcg gttctcgatg ctctgataaa
     2101 acaggttcac gcccttggcc cgcaccatgg actcgaccgc gggggcctgc gggaaatagg
     2161 tctcgatcga aatgccgtag cggtcccgga ccgcgtccat gacttcgtag gtctccgagt
     2221 gcaggcggcc ggtgtccaaa cagaagattc gcggcgcgcg gtcgatcttg cacatcatgt
     2281 ccaccaggac cacgtcttcc gcgccgaagc tgcacgccag cgccacctgc gagccgaact
     2341 ctttgagcgc ccacttgagc acggcttcag ccggctgggt cgtgtactgt tggttgaggg
     2401 tctgaagctg gtctttgctg tacagggtgg cggtgctcat gttcggatcg gctcctcggt
     2461 cgaaaggtct ctgttgaatt cagactgccc ggccggcgac agccacggcc ggtccgtcgg
     2521 attgcaattg tttggcgcga tcacacattt gctggagcgt gatggagccg aggacgtcgt
     2581 tgaccgagcc ctggaccgtg gtccaggcgt cccggatgac ccgaagattg ggcgtcgcgt
     2641 cggcgcacgc attggccgcc gaggcctcgg tccattggaa gaaagaccct tcggtcacgc
     2701 ggaaaatttc tcgcatggtg atcgaggtcg gatctttcga cagccggtac ccgcctttgg
     2761 cgccgcgaag gctctcaacc agcccgcgat gcttgaactc ggccacgatc tgctccagga
     2821 accgccgcgg gatgtcgaga tcatcggcca tggtggtgac cgccaccggg ccgctggccg
     2881 aatggcaggt cagatagagc aacgccaaca ttccgtactc gcttttggca gagattttca
     2941 acatcgtgaa attcctagtg atttgctcgg aattgtttca atataaaggg ggccggaaca
     3001 gcgtgtcaag tcaattgtgg cccgcggatc gcctggatcc gcggggttct tgtgggcagt
     3061 tccccggctt tgttatgtat aatagtccat acttatccat tgtgaggtcg attccgttta
     3121 gaacatgccc tggcagtcca tcgtagccat cctcgtcctg gtcttggcca acgggttttt
     3181 tgcggccgcg gagatagcga tcctgaccgt ccgcaagagc cggattctac acctggtcga
     3241 ggccggcaag caccgcgcca aagacgtcca gcggctccag gcggaccccg agcggtttct
     3301 cgccacgatc cagatcggca tgaccgtgat cagcgcgctg gccggcgccg tgggcggcgt
     3361 gaccgcgatc gagaacatca aaccctacct ggaagggatt ccgaacccgg tgttgcgcaa
     3421 cgcgagcgag ggcattgcgt tgaccatcgt ggtggcgtcg atctcgtatc tgtcgctggt
     3481 gttcggggag ttggtgccca agacgctggc gctgcggtat ccggagcaga tcgccttgtg
     3541 ggtctcgtgg ccgatcgacg ccctgtcccg cttctcgtca gtggtggtga aggtgctgat
     3601 gtggtccagc cggctgattc tcaagccgct cggcgcgaga tccaagcccg cgtttttccc
     3661 cgaagaggaa atcaagagtc tcttgatcga ggggcgccag accggggaga tcgatcggac
     3721 cgaacaggag ttgatccact cggtgtttga attcgccgac atctcggtca aggaggtgat
     3781 ggtcccgccg cccaagatcc acgcgatcaa gctcgacacg ccggttgaga cggtgctggc
     3841 cgatatcacg gagaacaagt tctcccggta tcccgtgtac cgcgaggagc tcaacgacat
     3901 ttgcggcgtg gtctacacca aggacattct cgacgccatg gtgcggcgcc ggccgatcga
     3961 catcaaatcc ctgatgcacc ccgcgtattt cgtgcctgaa agcatgaagg tgagccacct
     4021 gctgcaggaa atgcagcgcc ggcacaccca gatggcgatc gtggtcaacg agttcggcac
     4081 ggtcgagggc ctcgcgacca tggaggattt gatcgaggaa atcgtgggcg agatccggga
     4141 cgagtacgat ctggaagagc ggccggtcga gcgcctcaag gacggctcac tggtggtcga
     4201 cgcgtcgctg tcggtccggg atttgaggga agagtacggc ctgcccatcc ctgagtcgcc
     4261 tctgtacgag accctgggcg ggtttgtcat ggcccaattg cagaacatcc cgcgcagcgg
     4321 ggccattacc caccacaatc
//
LOCUS       DBNGTT010000328         5077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3012959, whole genome shotgun sequence.
ACCESSION   DBNGTT010000328 DBNGTT010000000
VERSION     DBNGTT010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3012959"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="AC16BC_07395"
     CDS             complement(<1..465)
                     /locus_tag="AC16BC_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR3241482.1"
                     /translation="MLATVRSSAVFGLDGYVVDVEVDIAQGLPMFSIVGLPDLAVKEA
                     KDRVRAAMKNTGLTFPIKRITVNLAPADVKKEGAGFDLPMAIGILVAEELLAAERVAR
                     FGLVGELSLDGRVRPVRGALPMALAARKFGLQGLILPRENAPEAAIVEGLSVY"
     gene            complement(617..1018)
                     /locus_tag="AC16BC_07400"
     CDS             complement(617..1018)
                     /locus_tag="AC16BC_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241483.1"
                     /translation="MKMDQFSTPVPGVDPEQPSAQRVTVDVTLVFRHADHPHTARIDF
                     TSASGLTTNNEVDLASVAPEVVAASSGRWVVRAPLIIPEPGTLRFHTVLVDQSGLAST
                     AVDGDFTVLSSFGSSNTIQASVETNTAPVSR"
     gene            complement(1255..2172)
                     /locus_tag="AC16BC_07405"
     CDS             complement(1255..2172)
                     /locus_tag="AC16BC_07405"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3241484.1"
                     /translation="MEAAARTIEAKAAMVFSGGGARGAYNAGVAMAFAKHGVYPEIVS
                     GTSVGALVAAMVVAGEEERLCEIWRHLTPSQVYGVRSRVQFFLSFIRPYRYPAYSSEP
                     LARLIQDNVALDKIRQSPRKLIVTCYNQTRHRVERFDNHCPDLTRVLLASTSIPLVFP
                     PVEMNGQEYVDLGSTVWPLKPAISAGAERIYAVLCDHPDDHRRAARNNLEWAIQVMNL
                     SQRSNMLVDIELARRINLTAHGSGYKFVDLTIIQPSKTLGLGFMDFHKKLQLNRALEM
                     GYEDATAALHDKPVSTPTGSLQGEFCPYI"
     gene            complement(2257..3402)
                     /locus_tag="AC16BC_07410"
     CDS             complement(2257..3402)
                     /locus_tag="AC16BC_07410"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3241485.1"
                     /translation="MRVIAIVNQKGGCGKTTTAINLAACLAAQSRNVLLIDLDPQAHA
                     TLGLSIKPEEAERGMYEVLVGEASIEEVINQILPNLSLAPSNIALSAIEQFLAGTPDR
                     ERQLAARVAALGDRFDYVLIDCPPSVGLLTFNALIACQEALVPVETSFFSLQGVVKLS
                     ETIKLISEKVGHDISVRVLPTKFDRRAGYAKEVLAKIREQFNSVTTATTIHLNDKLRE
                     AVSLGLPVTEYAPESAGSKDYMALAREIIAAEAFKGRATDVEIGFGPQSRSGVLFAVK
                     APGAHEVKLAGDFNGWVPDRNVLSLKEEGGVWKKFVFLAPGSYQYKYVIDNEWREDPH
                     NRLSVQDSLGGQSSVVVVEGMTVTLIKSHATAVGTTQALVESRPVTA"
     gene            complement(3764..4762)
                     /gene="bioB"
                     /locus_tag="AC16BC_07415"
     CDS             complement(3764..4762)
                     /gene="bioB"
                     /locus_tag="AC16BC_07415"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR3241486.1"
                     /translation="MDYEGIAERSLAGDLLSVDEGLELLAAPIDDLPTMLAAAFRVRR
                     ATFGRRVQFHVLMNAKSGLCPEDCGYCSQSSVAASDIERYPLRSQQEMLDAAKRAHAS
                     GAHRFCLVISGRGVTDGELDAISRAVQSVKAEVPIEVCCSLGLLDRAKAQRLREAGVD
                     RVNHNLNTSEGHYPRICTTHTYADRVATLEAVQEAGISTCSGVLAGMGESDRDLIAVA
                     RALRAMQVDSVPVNFLNPMPGTLLETMRSLTPWYCLKVLCLFRFVLPKTEIRIAGGRE
                     VNLRSLQPLALYPGNSLFINGYLTSDGQSADEAIRMVSDMGFEVDVLPARVSPVAS"
BASE COUNT          997 a         1669 c         1458 g          953 t
ORIGIN      
        1 atacaccgac aatccttcga cgatcgcggc ctcaggcgcg ttctcgcgag gaagaatcag
       61 gccttgaagt ccgaattttc gcgcggccaa cgccatgggc agcgcgcccc ggacgggacg
      121 gacccgcccg tcgagcgaca gctcgccgac cagtccgaat cgcgccacgc gttcagccgc
      181 caacagctct tccgcaacca gaattccgat tgccatggga agatcaaagc ccgctccttc
      241 ctttttgacg tcggcaggag caaggttcac cgtaatacgt ttgatcggga aggtcaggcc
      301 ggtgttcttc atggccgccc tgacccggtc cttcgcttcc ttcaccgcca agtcggggag
      361 gccgacgatg gaaaacatgg gtaggccctg cgcgatgtcg acctccacgt cgaccacgta
      421 cccatcgagc ccaaagaccg cgctgctccg caccgttgcc agcatcgtga tccctcgaac
      481 ggacgatctc acacccgagt gccagcgtca ccgcatcccc gtcgtgggga atacggcggg
      541 ggagtggacc acaggagaga ggaccagcga cagattggat cgaccgaagc gagccgcgcc
      601 gacaccccgg agcgggctac cgagaaacag gcgcggtgtt cgtttcgacg gatgcttgaa
      661 tggtattcga agagccgaac gacgataaga ccgtaaaatc cccgtccacc gcggtggacg
      721 ccagtccgct ctgatccacc aacaccgtat ggaatcgcag agtcccgggt tcggggatga
      781 tcagcggcgc acggaccacc caacgaccgc tgcttgccgc gaccacttcg ggcgccacgg
      841 acgccagatc gacctcattg ttcgtcgtga ggcccgacgc gctcgtaaag tcgatgcgcg
      901 ccgtgtgcgg gtggtcggcg tgccgaaaga ccagcgtcac gtccacggtg acccgctgcg
      961 ccgacggctg ctcgggatca acgccgggaa ccggcgtgct gaactggtcc attttcacaa
     1021 acgtcagctc cgtcccctgg gtgtcagagg acggttgctc gggcgctgtg cctcaagcgc
     1081 ccaccgcgag caagagcgcg aaggcgctga tgatcccgcc gcgtgaaatc ggccaaggca
     1141 acatgcttca ctctaaacca ccattttccc ggagtaaagg gccttttcca catggggcca
     1201 gccgagagcc tcctgcatca ccgagcggga tttggaacgt ggggagtgac gtcgctagat
     1261 gtacgggcag aattcgcctt gcagcgaccc cgtcggcgtg gacaccggtt tgtcgtgcag
     1321 cgcagcagtg gcgtcttcgt agcccatctc gagggcgcga ttgagctgga gcttcttgtg
     1381 gaagtccatg aagcccagtc ccagcgtctt ggacggctgg ataatggtca aatccacgaa
     1441 tttgtatccg ctcccgtggg cggtcaggtt gatgcgcctc gccaactcga tatccaccaa
     1501 catgttcgag cgctgggaga gattcatcac ctgaatcgcc cactccaggt tatttctggc
     1561 cgcgcggcga tgatcgtcgg gatggtcaca caatacggca tagatgcgtt cagccccggc
     1621 ggaaatcgcc ggcttgagcg gccaaaccgt cgaccccagg tcgacgtatt cctgcccgtt
     1681 catctcgacc ggaggaaaca cgagcggaat cgacgtgctg gcgaggagga cgcgcgtgag
     1741 atcgggacag tggttatcga agcgttcaac gcggtgccgc gtctggttat aacaggtcac
     1801 gatcagcttg cgcggactct gccggatctt gtccagcgcg acgttgtcct ggatcaaccg
     1861 agcgagcggc tcggagctgt acgcgggata acgatacggt ctgataaacg acaggaaaaa
     1921 ttggacccgc gatcggacgc catacacttg ggagggcgtc aaatgacgcc agatctcaca
     1981 cagacgttct tcctcgccgg ccacgaccat tgcggcgacc aatgcgccga ccgaggtccc
     2041 cgagacaatc tcgggataca ctccgtgttt ggcgaacgcc atggcgaccc cggcgttata
     2101 cgcgccccgg gcgcccccgc cggaaaacac catggcggcc ttggcttcaa tcgttctcgc
     2161 tgcggcctcc atgcctcaaa acgtagcgtc ccgcccgacg cctgtcaacg tcgggcggga
     2221 cgtcctcgtg caacgactcg cgcgagcggg gagagtttac gccgtgaccg gacggctctc
     2281 caccaacgcc tgcgtcgtcc cgacggccgt cgcatggctc tttatcagcg tgacggtcat
     2341 cccctcgacg accaccaccg aactctgtcc gccgagactg tcctggaccg acaggcggtt
     2401 gtgcggatct tcccgccact cgttatcgat cacatattta tattgatagc tgccgggcgc
     2461 caggaacacg aactttttcc atacgccgcc ctcctctttc agactcaaca cgttgcggtc
     2521 ggggacccac ccgttaaagt cgcccgccag cttgacctcg tgcgcgccgg gcgccttcac
     2581 tgcaaacagg acgccggatc gagactgcgg accgaatccg atctccacgt ccgtcgcgcg
     2641 ccccttgaac gcttccgccg cgatgatttc cctggcgagc gccatataat ccttggaccc
     2701 ggccgactcc ggggcgtatt cggtgaccgg aagtcctaaa ctcacggctt cgcgaagctt
     2761 gtcgttcagg tggatcgtgg tcgcggtcgt caccgaattg aactgttcgc ggattttcgc
     2821 gagcacctcc ttggcatatc ccgctcggcg atcgaacttg gtgggcaaca ctcggacgct
     2881 gatgtcgtgc cccactttct cgctgatcaa tttgatcgtt tcggagagct tgacgacgcc
     2941 ttggagggag aagaagctcg tttccaccgg caccaacgct tcttgacacg cgatcaacgc
     3001 gttgaaggtc agcaagccga cactgggcgg acaatcgatc agcacataat caaagcgatc
     3061 acccagggcg gccactcgcg ccgccaactg acgttcgcgg tccggcgtgc cggcgaggaa
     3121 ctgttcgatg gcgctgagcg cgatattcga cggcgccagc gacaagttgg ggagaatttg
     3181 attgatcact tcctcgatcg acgcctcgcc aaccaagacc tcgtacatgc cccgctcggc
     3241 ttcctcgggc ttgatgctca acccaagcgt ggcgtgagcc tgaggatcga ggtcaatcag
     3301 caacacgttg cgcgattgcg cggccagaca cgccgccaaa ttgatggccg tggtcgtctt
     3361 tccacacccg cccttctggt tcacgatcgc aatcacacgc ataacccacc ctccccaggg
     3421 ttagcggtcc tcgccgggac cgcagcagac ctatggcgcc cgaccatcga caacaccaat
     3481 gagatcacaa gtctcctgtt tcaacgtact ttcgtacggg gctcacctcc tttcttccga
     3541 tccacacctt gggaccagtg tgcccggcac gaaatccttg attttccaaa tgactctatt
     3601 aagcatgggt tggtaacaca atcgaattta agagtcaaga aaaaagataa cttatgagtt
     3661 tcttgtgctg attttgactg gaaagacccg ccggccgctc gaaaggcgcg agcactccac
     3721 ggcgacctca gacctggggg gaggaagcgt gtacggatgt gcgttacgat gcgaccggag
     3781 acactcgcgc aggcaggaca tccacctcga accccatgtc cgaaaccatc ctgatcgcct
     3841 cgtccgcaga ctgtccgtcg ctcgtcaggt acccgttgat gaacaaggag ttcccgggat
     3901 agagcgcaag cggttgtagg cttcggaggt tgacctcccg cccgccggcg attcggatct
     3961 cggttttggg caagacaaat cgaaacagac agagcacctt caaacagtac cacggggtca
     4021 gggaccgcat cgtctccaac agcgtgcccg gcatcggatt caagaagttc accggaaccg
     4081 aatccacctg catcgcacgc aacgcgcgcg ccaccgcaat cagatcccgg tcgctttccc
     4141 ccatccccgc caggacgccg ctgcacgtcg agatcccggc ctcctgaacc gcttcgaggg
     4201 tcgccacgcg gtccgcatag gtgtgcgtgg tgcagatccg cggataatgg ccttcgctcg
     4261 tattcaagtt gtgattcacc cgatcaactc cggcctccct cagccgctgc gccttagcgc
     4321 gatcgagcaa tccgagcgag cagcacactt cgatgggtac ctcggccttg accgattgca
     4381 cggcgcgcga gatcgcgtcg agttcaccat cggtcacgcc gcggccactg atcaccaggc
     4441 aaaaccgatg agcgcctgag gcgtgcgccc gcttcgcggc gtccagcatc tcctgctggc
     4501 ttctcaacgg gtatcgttcg atgtcggacg ccgcgaccga cgattgcgag cagtacccgc
     4561 agtcctccgg gcacagcccg ctcttcgcgt tcatcaacac gtgaaactgc acccgtcgtc
     4621 cgaacgtcgc ccgccgcacc cggaaggccg cggccagcat ggtcggcaga tcgtcgatgg
     4681 gagcggccag cagctccaac ccctcgtcaa cgctcaacag atccccggcc aacgatcgct
     4741 ccgcaatgcc ttcgtaatcc atactccgtc tccgcgccgc gcccgatact ggccttccta
     4801 tacgctgttt ggagtttttt gtcaatgcgg cgcccgcgga agcgccccct ttccggggcg
     4861 cgcggctgaa tatccggcga tctccggtgg tcttttccgg tgtgaagacg ctcagcctgg
     4921 gcatcttggg ggtcgtctcc tgcttgctcg ccgcctgcgg cgcgtggctc cgggcggaag
     4981 agcccgcccg aggcgggtcc acggcctcgt caggttgctc ggtattcggc caatcgctcg
     5041 actttgtcat cgataacaac ccatcgacca tcttctg
//
LOCUS       DBNGTT010000329         1668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_203904, whole genome shotgun sequence.
ACCESSION   DBNGTT010000329 DBNGTT010000000
VERSION     DBNGTT010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_203904"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16BC_07420"
     CDS             complement(<1..184)
                     /locus_tag="AC16BC_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endoglucanase"
                     /protein_id="HIR3241487.1"
                     /translation="MRETTGLRMTWVRGSAVALCALIAACGGGGGGDDSGGGGFQISP
                     STATVPAGGRMQFSGPS"
     gene            complement(177..1043)
                     /gene="panC"
                     /locus_tag="AC16BC_07425"
     CDS             complement(177..1043)
                     /gene="panC"
                     /locus_tag="AC16BC_07425"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR3241488.1"
                     /translation="MNSRLLVVSTSADMRRWSDAERRAGLSIGFVPTMGALHHGHASL
                     IRAARSACDRVVVSIFVNPTQFGPQEDYGRYPRTFEADRALCEREGADAVFVPEVADM
                     YPPGAETIVDVGALGTVLCGASRPGHFRGVATVVTKLFAIVAPHRAYFGQKDFQQTVV
                     IRRLVEDLRLGVEVVVCPTVRDPDGLAASSRNTYLAPEERKTALRVPEALAEAGRLIA
                     AGERSGGKIRDALEVFLSSVPALRVEYAAVVHPDTLQPVERLSGRVVVALAVRVGNTR
                     LIDNAVFEVQDA"
     gene            complement(1123..>1668)
                     /locus_tag="AC16BC_07430"
     CDS             complement(1123..>1668)
                     /locus_tag="AC16BC_07430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3241489.1"
                     /translation="TPILEESENNSFLIKFYRDRRRWAFQTQLFFLLSRYRQQEEMAQ
                     HELFSNVTITDVFLPKDRIFASLNLTPDELVLYDQIYGLLRSRLPKPDLVIYLHAETN
                     ALMQRVKQRGREYEQEMTWEYLDELSRTYNEFFFSYNDTPLLVIQTTEIDFVNNPADL
                     SDLIRQIRQMKGGVQYYVPAG"
BASE COUNT          305 a          568 c          510 g          285 t
ORIGIN      
        1 cgctggggcc gctgaactgc atgcgtccgc cggccggtac cgtggcggtg ctcggcgaga
       61 tttgaaatcc gccacctccg gagtcgtctc cgccgccccc cccgccgcac gcggcgatca
      121 gggcgcacaa ggccacggcc gatcccctga cccacgtcat ccgcagaccc gttgtctcac
      181 gcatcttgga cctcaaagac ggcattgtcg atcaggcgcg tgttgcccac ccggacggcc
      241 agcgccacga ccacccgtcc gctcaaccgc tctaccggct ggagcgtgtc cggatgcacc
      301 accgcggcgt actccacccg gagcgcggga acgctcgaca ggaagacctc caacgcgtcc
      361 cgaatcttcc cccccgagcg ctccccggcc gcgatcaaac gcccggcttc agccaatgcc
      421 tcgggcaccc tcaacgcggt ttttcgctcc tccggcgcga gatacgtgtt gcgggagctg
      481 gcggccaggc cgtcggggtc ccgcaccgtg gggcacacca ccacctcgac acccagccgc
      541 aaatcctcga ccaggcgccg aatcactacg gtttgctgga agtccttttg gccgaaatag
      601 gcgcggtggg gcgcgacgat cgcaaacaat ttggtgacca cggtggccac cccgcgaaaa
      661 tgtccggggc gagacgcccc gcataggacc gttccgagcg cgcccacgtc cacaatggtc
      721 tccgcgcccg gggggtacat gtcggcaacc tcggggacga acaccgcgtc cgcgccctct
      781 cgttcacaca gggcgcgatc cgcctcgaac gtgcgtgggt agcggccgta atcttcctgg
      841 ggcccgaact gcgtggggtt cacaaagatg ctcacgacca cccgatcgca ggcggaccgc
      901 gccgcgcgga tcaggctcgc gtggccgtgg tgcaaggcgc ccatggtggg cacgaacccg
      961 atgctcaggc cggcgcgccg ttccgcgtcc gaccagcgtc gcatatcggc cgaggtggag
     1021 accaccaaga gtcgagagtt catggcgcgc cgctcacctc gacggcgcga cgtcgatcgc
     1081 ccgccctctt gttgagccgc gggctatgag ctgcgacccg attcaccccg ccgggacata
     1141 gtactgcacc ccgcccttca tctgccggat ttggcggatc aggtccgaga ggtcggccgg
     1201 gttgttcacg aagtcgatct ccgtggtctg gatcacgagg agcggggtgt cgttgtagga
     1261 aaaaaagaac tcgttgtacg tccgactcaa ttcgtccagg tactcccacg tcatctcctg
     1321 ttcgtactcc cgcccgcgct gtttaacgcg ctgcatgagc gcattggttt ccgcgtgcag
     1381 gtaaatcacc agatccggtt tgggcagccg gctacgcagc agaccgtaga tctggtcgta
     1441 caagaccaac tcatccgggg tcaggttcag cgacgcgaag atacgatcct tgggcaggaa
     1501 gacatccgtg atcgtgacgt tggagaagag ctcgtgttga gccatctcct cttgttggcg
     1561 ataacgcgag aggagaaaaa agagctgggt ctggaacgcc caccgccggc gatcgcgata
     1621 aaatttgatc agaaacgaat tgttctcgga ttcttccagg atcggcgt
//
LOCUS       DBNGTT010000330         6240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_459558, whole genome shotgun sequence.
ACCESSION   DBNGTT010000330 DBNGTT010000000
VERSION     DBNGTT010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_459558"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1144)
                     /gene="waaF"
                     /locus_tag="AC16BC_07435"
     CDS             complement(59..1144)
                     /gene="waaF"
                     /locus_tag="AC16BC_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR3241490.1"
                     /translation="MTIAAPRALLVCAPNWIGDAVMATPTLAALRAGFPDARITVLAR
                     AGVVDVLTGHPAIDEVMLDDRRGRHRGAAGRLRLASDLRGRGFDGVLLLPNSFASALV
                     AALARVPARVGYRTDGRGALLTTALAPPRKPAPHMTAYYLGLLAPWALAGDPRAVTLA
                     VTDAERDAASRRLAEWGIGREERVVAVNPGAAYGSAKRWPSDRFAAVSRRLVQDGAAV
                     LVLGAASERGLADAVVAGLGRRAVNAAGMTTMREAMALMARCGHVVTNDSGPMHVAAA
                     LGVPVTAVFGPTEPRATSPVGERVTILQRPVDCAPCRYRECPIDHRCMTGVGVDEVYT
                     AVASAASTHPPVLPLSKEGRGGCSGEP"
     gene            complement(1141..2190)
                     /gene="lpxK"
                     /locus_tag="AC16BC_07440"
     CDS             complement(1141..2190)
                     /gene="lpxK"
                     /locus_tag="AC16BC_07440"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR3241491.1"
                     /translation="MRWGLAPLSWVYGGVVRARRWWYERGWGRVHALGRPVVSVGNLS
                     VGGTGKTPVVIAISEALLARGVGVTVLSRGYRGMSERGGALVSDGRGAARAGWEQAGD
                     EPALIARRAPGASVMVGRDRFATWRRFGPGLPPGVVVLDDGFQHLAIARDENLALLDA
                     TDDDSRLALLPRGRLREPWSALSRATAVVITRVDQAAPGAVAEVERRVREQRPDIPLV
                     HARFAPTDLREVATGRSRPLDDLRGAPVLAVAGIGRFESFPAMLERCGARPVGVMPFP
                     DHHAYSRADADRIAARARRAGARMIVTTEKDAVKLERLVGVDPPMWAVRIDARIEPAA
                     VWNAMLDRLAAGARA"
     gene            complement(2396..3700)
                     /locus_tag="AC16BC_07445"
     CDS             complement(2396..3700)
                     /locus_tag="AC16BC_07445"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF016311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR3241492.1"
                     /translation="MGLIRPLYTAALAVGLTITAPAYAVRAARRGTGLALGERLGAVP
                     AAAAALRGRRPLWVHAVSVGEVTAAAPLLRAIKTRYPDVALLVSTVTATGRQTVTERV
                     PQADATVYFPLDLPAAVARALDAVEPGLFLSVETELWPNFLHALAGRGVPAFLVNARL
                     TERSARRYGWAGAVFRPALAGLSGVAAQPAVDAKRFASIGADPNRILITGNLKFDQGV
                     QEADGAIRRDELGLAPEDRLWVAGSTHPGEETQLLDVYRRVRTREPRLVLLLAPRHLD
                     RLAQVEALVRDAGCEPIRRSLARGATRTESRPPVIVLDTLGELAGLYHEAAVAFVGGT
                     LVPVGGHNLLEPAARGKPVLFGPHTHKCAEIAQALLDAGGAARVDSVEAMARDLERLL
                     ADERLRVRMGEYGRDMVARNRGAVERTLAMLDPWLSELGARP"
     gene            complement(3691..3843)
                     /locus_tag="AC16BC_07450"
     CDS             complement(3691..3843)
                     /locus_tag="AC16BC_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241493.1"
                     /translation="MPYPFSRGVFIWGEPIWVSREADAEESEARRADVQRALNQITER
                     ADHWWD"
     gene            complement(3876..4343)
                     /locus_tag="AC16BC_07455"
     CDS             complement(3876..4343)
                     /locus_tag="AC16BC_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3241494.1"
                     /translation="MSAREPLWRRAAPPLVAALIRAIGRTMRIETINQAAIAERWREG
                     QGNILAFWHGRQFLLPLSYQGPGLDILISRHRDGELISRAMQSFGLSSVRGSTTRGGA
                     VALRGLIRKGRAGRDLAVTPDGPKGPRGIAQPGVIALAKATGLPIFPLTFGAS"
     gene            complement(4330..6096)
                     /locus_tag="AC16BC_07460"
     CDS             complement(4330..6096)
                     /locus_tag="AC16BC_07460"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3241495.1"
                     /translation="MTAPAAGARSVYGRIARLIAPHWRRVLAAGLCAAGVSGLTATNL
                     WLVQKVLDRIFIDKHSAWLVPLAIAIPAVALAKRVFQLGYVYLMGHTANTIITDIRDA
                     LYRHILKLSMGFHTARSTGGLMSNVTNDVGQMQRAVGVVKDLVQQPLTLAAMLGFIVY
                     QNWRMALMAIAVLPFGYYPMIRLGRRLKRLAHDGQERMAELSGMLQETFAGISVVKGF
                     GREEFEAERVRRLNRRYFRNAMKVTVVSEFAPALMEVLGAVGAGAVVAYGGYAVISGQ
                     MTTGAFFSFLGACWLTYAPMRVLSGANNTLLTAAASAERVFSLMDLETEERRDRGTRE
                     LDRVRDRVEYRGVGFTYEGAERPALADVSLTARAGEVVAIVGSSGSGKTTLVNLLPRF
                     VEPTQGAILIDGVDLRDIRLASLRSRIGIVSQDVILFDDTIRNNIAYGVSRVDEGEVQ
                     RAAEAAFAHGFIQRLPGGYDTVIGERGVMLSGGERQRIAIARAILKNPPILILDEATS
                     ALDPESEAAVQQALANLMAHRTTFVIAHRLSTVQRATRIIVLDRGRIVEEGTHDSLLA
                     KQGAYARLYRIQFDTEAGYVGS"
BASE COUNT          964 a         2434 c         2028 g          814 t
ORIGIN      
        1 ccctcgtcga ggttgagggt gccgtcgcgg tcgaggaata cgatgtcgag cgggcggctc
       61 atggttcacc tgaacatccc ccccggccct cctttgacaa cgggaggacg gggggatggg
      121 tcgatgcggc gctcgcgacc gccgtgtaga cctcgtccac tcccaccccg gtcatgcacc
      181 gatggtcgat cgggcattcg cggtaccgac agggcgcgca gtccaccggc cgttgaagaa
      241 tcgtcacccg ttcgcccacc gggctcgtgg cccggggctc ggtggggccg aacaccgcgg
      301 tcacgggcac gcccagcgcc gcggccacgt gcatgggccc ggaatcgttc gtgaccacgt
      361 gcccgcaccg ggccatcaac gccatggcct cgcgcatcgt agtcatgccg gccgcgttta
      421 ccgcccgacg gccgaggccc gccaccacgg catcggccag cccccgttcc gaggccgctc
      481 ccagcacgag cacggccgcg ccgtcctgaa cgagtcgccg cgacacggcc gcgaaccggt
      541 ccgagggcca ccgcttggcc gaaccgtacg cggcgccggg attgacggcc acgacgcgtt
      601 cttcgcgtcc gatcccccat tccgccagcc gccgagacgc ggcgtcgcgc tccgcgtcgg
      661 tcaccgccag cgtgacagcc cgcgggtccc cggccagggc ccacggggcc agcagcccga
      721 ggtagtacgc ggtcatgtgc ggcgcgggct ttcggggcgg cgcgagggcc gtggtcagca
      781 acgcgccccg gccgtccgtg cgatacccca cccgcgcggg cacgcgggcc aacgccgcca
      841 ccagcgcgga cgcgaacgag ttgggcagca gcaacacgcc gtcgaatccg cggccgcgaa
      901 gatccgatgc gaggcgcagc cggcccgccg cgccacggtg ccggccccgg cgatcgtcca
      961 gcatcacctc gtcgatggcc gggtgtccgg tcaacacgtc cacgacgccc gcgcgcgcca
     1021 acaccgtgat cctggcgtcg ggaaacccgg cgcgaagcgc cgcgagggtg ggcgtggcca
     1081 tcaccgcgtc cccgatccag ttcggcgcgc agaccaggag cgcgcggggg gcggcgatcg
     1141 tcacgcgcgc gccccggccg ccagccgatc cagcatcgcg ttccagaccg ccgcgggttc
     1201 gatccgcgcg tcgatgcgca ccgcccacat cggcgggtcg acgccgacga gtcgttccag
     1261 cttgaccgcg tctttctcgg tcgtcacgat catccgcgcc ccggcccggc gcgcgcgggc
     1321 cgcgatgcga tccgcgtccg cccgggaata cgcatggtga tccgggaacg gcatcacgcc
     1381 gaccggacgc gcgccgcacc gttcgagcat ggccggaaac gattcgaacc ggccgatgcc
     1441 cgccaccgcc aggacgggcg cgccccgcag gtcgtcgagc ggacggctgc ggccggtcgc
     1501 cacctcgcgc aggtccgtgg gcgcgaaccg cgcgtgcacc agcgggatat ccgggcgttg
     1561 ctcgcgcacc cgccgctcca cctccgccac cgcgccgggc gcggcctggt ccacccgggt
     1621 gatcacgacc gcggtcgcgc gcgacaacgc ggaccagggt tcccgcaacc gtccgcgggg
     1681 cagcagcgcg aggcgcgagt catcatcggt cgcgtccagc agcgccaggt tttcgtcccg
     1741 cgcgatcgcg aggtgctgaa acccgtcatc aagcaccacg acgccgggcg gcaggccggg
     1801 tccgaaccgt cgccacgtcg caaatcgatc gcgccccacc atgaccgacg cgcccggcgc
     1861 gcgccgcgcg atgagcgcgg gctcgtcgcc cgcctgctcc caccccgccc gggcggcgcc
     1921 gcgtccgtcc gacaccaggg ccccgccgcg ctccgacatc ccccgatacc cgcgactcaa
     1981 caccgtgacc ccgaccccgc gcgccagcag cgcttcgctg atcgcgatca ccacgggcgt
     2041 tttccccgtc ccccccaccg acaaattccc caccgacacg accggccgcc ccaacgcatg
     2101 aacgcgtccc cacccacgct cgtaccacca gcgtcgcgct cgcacgacgc cgccatacac
     2161 ccacgacaac ggcgcaagcc cccacctcat cgttcagcgc tctcggttga ggattgccat
     2221 cgccccatct cactactagc tatccgctgt gggcctcggt tctcgtctct cacaaccgcg
     2281 aagcggagcc cgtccagaag cgggccattc gcaaggcgga gcgagcgccc gcaccgcagc
     2341 gtactggctc gtacgtgagg agcggacgcg cagcgacaac gccgcagatg gccccttatg
     2401 gacgggctcc cagctcggac agccagggat cgagcatcgc cagggtcctc tccaccgccc
     2461 cgcgattgcg cgccaccatg tcgcgtccgt attcgcccat ccggacccgg agccgctcgt
     2521 cggccaacag ccgttcgagg tcgcgcgcca tggcctcgac cgagtccacg cgcgcggccc
     2581 cgcccgcgtc cagcagggcc tgggcgatct ccgcgcactt gtgcgtgtgc ggcccgaaga
     2641 ggaccggctt gccgcgcgcc gcgggctcca acagattgtg tccgccgacc ggcaccaacg
     2701 tgccgcccac gaacgccacc gcggcttcgt gatacaaccc ggccaattcc cccagggtat
     2761 cgagcacgat caccggcggc cgggactccg ttcgcgtcgc accccgcgcg agcgatcgtc
     2821 ggatcggctc acagcccgcg tcccgcacca gggcctcgac ctgggcgagg cgatccagat
     2881 gtcgcggcgc gagcaggagc accagccgcg gctcgcgggt ccgcacgcga cggtacacgt
     2941 cgagcagttg cgtctcttct ccggggtgcg tgctcccggc gacccacaac cggtcctcgg
     3001 gcgccaggcc caactcgtcg cgtcggatcg ccccgtcggc ctcctgcacg ccctggtcga
     3061 atttgagatt gccggtgatg aggatgcggt tcggatccgc gccgatcgac gcgaagcgct
     3121 tcgcgtccac cgcgggctgg gcggccaccc cggacaggcc ggccaacgcc ggccggaaca
     3181 ccgcccccgc ccagccgtac cggcgcgcgg accgctcggt cagccgggcg ttcacgagga
     3241 acgcgggcac gccgcggccc gccagcgcgt gcaggaagtt cggccacaat tcggtttcaa
     3301 ccgacaggaa caacccgggc tccacggcat cgagcgcccg ggccacggcc gccggcaggt
     3361 ccagcgggaa atacacggtc gcgtcggcct gaggcacgcg ctccgtcacg gtctggcgtc
     3421 cggtcgccgt caccgtgctg accagcaggg cgacgtccgg ataccgcgtt ttgatcgcgc
     3481 gcagcagcgg cgccgcggcc gtgacctcgc ccaccgacac cgcatggacc cacaagggcc
     3541 ggcgaccgcg caacgccgcg gccgcggccg ggaccgcgcc caaccgctcg cccagcgcca
     3601 gtccggtgcc gcgccgagcc gcccggaccg cgtacgccgg cgcggtgatt gtcagcccca
     3661 cggccagcgc cgcggtatac aagggccgga tcaatcccac cagtgatccg cgcgttccgt
     3721 gatctggttc aacgcccgct gcacgtccgc gcgtcgggcc tcggactcct ccgcgtccgc
     3781 ctcgcgagac acccagatgg gctcgcccca gatgaagacc ccgcgcgaga acgggtacgg
     3841 caccacgaag ccgtcccagg tatcaagggt tttttttatg aggccccgaa ggtaaggggg
     3901 aagatgggca ggccggtcgc cttggccaac gcgatcaccc cgggttgcgc gatcccgcgc
     3961 gggcccttgg gcccgtccgg cgtcaccgcc agatcccgtc ccgcccgtcc cttgcggatg
     4021 aggccccgga gcgccacggc gccgccgcgg gtggtggacc ctcggaccga cgagagcccg
     4081 aacgattgca tggcgcggct gatcagttcc ccgtcgcggt ggcggctgat caggatgtcc
     4141 agcccgggcc cctgatagga caagggcagc aggaattgcc gaccgtgcca gaacgccagg
     4201 atgttgccct gtccctcgcg ccaccgctcg gcaatggcgg cctgattgat ggtttcgatc
     4261 cgcatggtcc gtccgatggc gcgaatcagc gccgcgacca acggcggcgc ggcccgccgc
     4321 cagagcggct cacgagccga catacccggc ctccgtgtcg aactggatcc ggtacaggcg
     4381 cgcgtacgcg ccctgcttgg ccagcagact gtcgtgcgtg ccctcctcca cgatccgccc
     4441 gcggtcgagc acgatgattc gcgttgcccg ctggaccgtc gacagccggt gcgcgatcac
     4501 gaacgtggtg cgatgcgcca tgagattggc caatgcctgc tgcacggcgg cctcggactc
     4561 ggggtcgagc gccgaggtcg cctcatccag aatcagaatc ggggggttct tcaagatcgc
     4621 ccgcgcgatc gcgatccgct ggcgttcgcc gcccgacaac atgacgccgc gctccccgat
     4681 caccgtgtca tagcccccgg gcagccgctg gatgaatccg tgcgcgaacg ccgcctccgc
     4741 ggcccgttgc acctcgccct cgtccacgcg gctgaccccg taggcgatgt tgttgcgaat
     4801 cgtgtcgtcg aacagaatca cgtcctggct cacgatgccg atgcgcgatc gcagcgacgc
     4861 caggcggatg tcccgcaggt ccacgccgtc gatcagaatc gcgccctggg tgggctcgac
     4921 aaaccgcggc aacaggttca ccagcgtggt ctttccgctg ccgctcgagc ccacgatcgc
     4981 gaccacctcg cccgcgcgcg cggtcaagga gacgtcggcc agggccgggc gctcggcgcc
     5041 ctcgtacgtg aagcccacgc cccgatactc gacgcgatcg cgcacccgat cgagctcgcg
     5101 cgtgccccgg tcccgccgct cctcggtctc cagatccatc aggctgaaga cgcgctccgc
     5161 ggacgccgcc gccgtgagca gggtattgtt cgcgccggac aacacccgca tcggcgcata
     5221 cgtcaaccaa cacgccccca ggaacgaaaa aaacgcgccg gtcgtcatct gcccgctgat
     5281 caccgcgtag ccgccgtacg ccaccaccgc gcccgcgccc accgcgccca ggacctccat
     5341 cagggcgggc gcgaactcgg aaacgaccgt gaccttcatc gcgttccgga agtaccgccg
     5401 gttgagtcgg cgcacgcgct cggcctcgaa ctcctcgcgc ccgaatccct tgacgaccga
     5461 gatgcccgcg aaggtttcct gcaacatccc ggacaactcc gccatgcgct cctgcccgtc
     5521 gtgggcgagc cgcttgagcc gacggcccag gcggatcatg gggtaatacc cgaacggcag
     5581 cacggcgatc gccatcagcg ccatccgcca gttctggtag acgataaacc cgagcatggc
     5641 cgccagcgtg agcggctgct gcaccaggtc cttgaccacg cccaccgcgc gctgcatctg
     5701 ccccacgtcg ttggtgacgt tcgacatcaa gcccccagtc gaacgcgcgg tgtgaaatcc
     5761 catggacagt ttgaggatgt gccgatacaa cgcgtcgcga atgtccgtga tgatggtgtt
     5821 ggcggtgtgc cccatgaggt agacgtaccc caactggaac acccgtttgg ccagcgcgac
     5881 cgcggggatg gcgatcgcca gcggcacgag ccacgcgctg tgtttgtcga tgaagatccg
     5941 gtccaggacc ttctggacca accagaggtt ggtcgcggtc aggccggaca cgcccgcggc
     6001 gcagaggccg gccgccagca cgcggcgcca gtgcggcgcg atcagccgcg cgatgcgccc
     6061 gtagacgctc cgcgctcccg cggcgggagc cgtcacgcgg cgcgcctccc ggccagcacg
     6121 gccagcaccg cctcggccgc gcgggcggac gcgccgggct cccccaacgc ggcgcgcacc
     6181 gcggccaact cccgccgcat cgcctcgttc gcgcggtcat cggtcaacaa gggcgccacc
//
LOCUS       DBNGTT010000331         2240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_843759, whole genome shotgun sequence.
ACCESSION   DBNGTT010000331 DBNGTT010000000
VERSION     DBNGTT010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_843759"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..768
                     /locus_tag="AC16BC_07465"
     CDS             202..768
                     /locus_tag="AC16BC_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3241496.1"
                     /translation="MGVAIETLKKEHAAIERMLAILEIVCRRLQAGELVDPAHVGQIV
                     EFFHGFADECHHGKEETVLFPALEKAGIPREGGPVGVMLYEHDQMRGFLRGVSEAVER
                     LRAGEVGAEGDIVRHAGDYAGTLRRHIDKENTVLFRMAEMRLGPDQEKRLAEQFEVVE
                     TRKMGAGKHEELHALLDRLEGIYLRGKT"
     gene            1122..1508
                     /locus_tag="AC16BC_07470"
     CDS             1122..1508
                     /locus_tag="AC16BC_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3241497.1"
                     /translation="MRACWEAIPEHLQQTTVDTFVVMPNHVHGVVWVHGEGRGTACRA
                     PTADRVEEFGRPVAGSLATIVRSFKAAVTKRINARRHSSGLPVWQRNYYEHVIRAENE
                     LARVREYIRDNPRAWDSDPENPSIVR"
     gene            complement(1546..1752)
                     /locus_tag="AC16BC_07475"
     CDS             complement(1546..1752)
                     /locus_tag="AC16BC_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241498.1"
                     /translation="MANRDLKILLEKVEGGYIVADPNPDPKTPTDVWINKAVHTDFDS
                     AMEDVRRRLGVSDIIASHEGKLSI"
     gene            complement(1788..2204)
                     /locus_tag="AC16BC_07480"
     CDS             complement(1788..2204)
                     /locus_tag="AC16BC_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor family protein"
                     /protein_id="HIR3241499.1"
                     /translation="MSFPVSPQKNDELTARMAKLGVREADLDETFVRGSGPGGQHVNK
                     TATCVMLVHRPSGTSVRCQASRSQGLNRFYARRLLLDKIERQQLGQASAAQQEIEKIR
                     RQKRKRSKRAKAKMLDEKRQHAEKKATRGRVRPNSD"
BASE COUNT          416 a          606 c          729 g          489 t
ORIGIN      
        1 cgcatgtttc cgcggtctca cagccgcggc ctcattgaag gaaccaaccg gggacgttct
       61 tttaagattt aaacttttcg acctttgtat tgtgatcgat ttactcgccg agttagtatt
      121 gagcctcgtt gtacagcaac tgtcgttttc aaccttgaac tttgaacatt aaaccttgaa
      181 ccagcgcacc ggaggcgtga catgggcgtg gcgattgaga cgttgaagaa ggagcatgcg
      241 gcgattgagc gaatgctcgc gattttggaa atcgtgtgtc gacggcttca ggcgggcgaa
      301 ttggttgatc cggcgcacgt ggggcagatc gtggagttct ttcatgggtt cgcggacgag
      361 tgccatcacg gcaaggaaga gacggtgctg tttcccgcgt tggagaaggc aggaattccc
      421 agggagggcg ggccggtggg tgtgatgctc tatgagcatg atcagatgcg cggattctta
      481 cgcggtgtga gtgaagcggt ggaacgcttg cgcgcggggg aggtgggcgc ggagggggac
      541 attgttcgcc acgccgggga ctacgccggc acgttgaggc gacacatcga caaggaaaac
      601 accgtgctct ttcgcatggc cgagatgcgc ctggggccgg atcaggagaa gcggcttgcc
      661 gagcagttcg aggtggtcga aacccggaag atgggggcgg gcaaacacga ggaactccac
      721 gcgctgctcg acagactgga aggaatctat cttcgcggaa aaacgtaggg gcgcggcatg
      781 ccgtgcccct actggccaga aagggccaat tgacctctgt cacaccttat atataatgat
      841 gcgccctgag acaaggcacg gcatgccgtg cccctacggt cggctgaaat cgacgcttga
      901 gaagtaacca ccacggatct gtttccgcgt tgcgccgcgc ctcgcggagg gtcacgtgtc
      961 ctataacccc gaacgacacc ctcgtcggtc tgtacggtta cctggccatg attacgcaac
     1021 cgccggcgcg tatttcatca cgatgtgtac gtggaaccga gagtgtctct ttggcgatgt
     1081 tatcggcggc gagatgacac tgaacgaatt atggagaggt cgtgcgcgcc tgttgggaag
     1141 cgatccctga acatcttcag cagacgacgg tggacacctt tgtggttatg ccgaatcacg
     1201 tacacggcgt ggtgtgggtc catggtgagg gtaggggcac ggcatgccgt gcccctacgg
     1261 ccgatcgtgt cgaagaattc ggacggccgg ttgccgggtc gttagcaaca atcgtccggt
     1321 ccttcaaagc cgccgtgacg aaacggatca atgcccggcg ccacagttcc ggtcttccgg
     1381 tgtggcagcg caactattac gagcacgtga tccgggctga gaatgaattg gctcgggttc
     1441 gcgaatatat tcgtgacaac ccccgggcat gggattccga tccggaaaac ccctcgatcg
     1501 tcaggtaggg gcacgacatg tcgtgcccct acggacggag ggcaattaaa tcgacagctt
     1561 gccttcgtgc gacgcgatga tgtccgacac gcccaatcgc cggcgcacgt cttccatcgc
     1621 ggagtcgaag tccgtgtgca cggccttgtt gatccagacg tcggtcgggg ttttggggtc
     1681 ggggttgggg tcggccacga tgtagccgcc ctcgactttt tccagcagga ttttcagatc
     1741 gcgattggcc ataccaaggg gattctataa gcgcctgagt gaggctgtca atcgctgttc
     1801 ggtctgacgc ggccccgcgt cgcctttttc tccgcgtgtt gccgcttctc atccagcatc
     1861 ttggccttgg cgcgtttcga gcgtttccgt ttctggcggc ggatcttttc gatctcctgt
     1921 tgcgcggcgc tggcttggcc cagttgttga cgttcgatct tgtcgagcag gaggcggcgc
     1981 gcgtagaagc gattgaggcc ttgcgagcgc gaggcctggc agcggacgct ggttccgctc
     2041 gggcggtgca ccagcatgac gcacgtggcg gtcttgttca cgtgctgccc gccggggcct
     2101 gatccgcgca cgaaggtttc gtcgaggtcc gcctcgcgca ccccgagctt ggccatccgc
     2161 gcggtcaact cgtcattctt ttgcggcgac accggaaagg acatctggga tctcctggtt
     2221 ggccggtcta tgggttagcc
//
LOCUS       DBNGTT010000332         1861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_587576, whole genome shotgun sequence.
ACCESSION   DBNGTT010000332 DBNGTT010000000
VERSION     DBNGTT010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_587576"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="AC16BC_07485"
     CDS             <1..1086
                     /locus_tag="AC16BC_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR3241500.1"
                     /translation="YTVADRSTGRLMVLRPDVTPQIARMAATVLRDAPKPLRLYYGAN
                     VFRYEDEHAGHEREIFQLGAELIGPSDPSADAEVLALAVDLLGRFGLPRPTVAIGHVG
                     FFLGWLADADVSPDRAHALEEAVAKKDRSGVVEVLVASGRSRAAASRLAGLLDLIGSS
                     EVLERAPSVVDNAPAREAVASLREIARQARDYGIAGALVFDLSEIRGREYYSGLIFDL
                     FADGVGYEIGRGGRYDELIGRFGPATPSTGFAIHLERLQQSLGTPPDRPPPGPLDALV
                     VATSGVREAIRLAGTLRRGGRRVALWTTADGAPLAYAKARNIPWLVSPGPSRDRVALT
                     SAATGWTRRITVAACSTAIAGRRGASS"
     gene            1140..>1861
                     /locus_tag="AC16BC_07490"
     CDS             1140..>1861
                     /locus_tag="AC16BC_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HIR3241501.1"
                     /translation="MDQLISAERVPPQHLEAEEFVLGAILLDNQAMNKVLEVLSPTAW
                     YREAHRKIFQAMVDLSEANEAIDQITVCERLRRRNELDQVGGASYLAHLVAQVPTAAN
                     VRHHAKIVQEKALLRNLIAVATDIVASGYQDTEKAEDLIDQAERKIFELAERRMRQAF
                     VPVRQIVKGAFEMIERLYEKKERVTGVPTGFTELDEMTAGLQPSDLIIVAGRPSMGKT
                     ALALSMAQHAAIHKRQTVAVFSL"
BASE COUNT          307 a          582 c          653 g          319 t
ORIGIN      
        1 tacacggtgg ccgaccgcag cacggggcgc ctcatggtgt tgcggccgga cgtgacgccc
       61 cagatcgcgc ggatggccgc caccgtgttg cgcgacgccc ccaaaccgct tcggctctac
      121 tacggcgcga atgtctttcg ttatgaggac gaacacgcgg gacacgagcg cgaaatcttt
      181 caactcggcg ccgaattgat cggtccgtcg gacccgtcag cggacgcgga agttctcgcg
      241 ttggcggtcg acctcctcgg ccggttcggg ttgccgcgcc ccaccgtggc gatcggccac
      301 gtgggattct ttcttggatg gctggccgac gccgatgtgt cgccggaccg cgcgcacgcg
      361 ctcgaggaag ccgtcgcgaa aaaagaccgg agcggcgtgg tcgaagtgct ggtggccagc
      421 ggccggtcgc gagccgccgc gtcgcgcttg gcgggcctct tggacctgat cgggtcctcc
      481 gaggtgctcg aacgcgcgcc gtccgtggtg gacaacgcgc ccgcgcggga ggcggtggcg
      541 tcgctgcggg aaatcgcccg acaggctcgc gactacggta tcgccggggc gctggtgttc
      601 gatttgtcgg agattcgcgg acgagaatac tactccggtc tcatcttcga cctgttcgcc
      661 gatggcgtgg gttatgagat cggtcggggt ggtcggtacg acgagttgat cggacgattc
      721 ggcccggcca cgccgtccac cgggtttgcc attcatctcg agcggctgca acaaagcctg
      781 gggacgccgc ccgatcgtcc tccgcccggg ccgctcgacg cgctggtcgt ggcgacgagc
      841 ggggtgcggg aggcgattcg tcttgccggg acccttcggc gcggagggcg gcgcgtcgcg
      901 ttgtggacga ccgccgacgg cgcgccgctg gcgtacgcga aggcgcgaaa catcccgtgg
      961 ctggtgtcgc cgggcccgtc ccgtgatcgg gtcgcgttga cgtcggcggc aaccggatgg
     1021 acgaggcgga tcacggtcgc ggcgtgttcg accgcgattg ccggacgtcg cggagcgtcg
     1081 tcgtgatgcc ccgtcgcatc acgcgattcg gaataatcct ccgcgcgaga gcgtgacgca
     1141 tggatcaact gatcagcgcc gaacgggtcc ccccgcaaca ccttgaagcc gaggagttcg
     1201 tgctcggcgc gattctgctc gacaaccagg ccatgaataa agtgctggaa gtcctgtcgc
     1261 ccaccgcgtg gtaccgcgag gcgcaccgga agatctttca ggccatggtg gatctctccg
     1321 aggcgaacga ggccatcgac cagattacgg tgtgcgagcg attgcggcgc cgtaatgagt
     1381 tggaccaggt cgggggcgcg tcctatttgg cgcacttggt ggcgcaagtc cccacggcgg
     1441 ccaatgttcg acatcacgcc aagattgtcc aggaaaaggc gctgctccgg aacctcatcg
     1501 cggtcgccac cgacatcgtg gcctccggat accaggacac ggagaaagcc gaagacctga
     1561 tcgaccaggc cgagcgcaag atcttcgaat tggcggaacg tcggatgcgt caggcattcg
     1621 ttcccgtgcg gcagatcgtg aagggcgcgt tcgagatgat cgaacggctc tacgagaaga
     1681 aggagcgcgt gaccggcgtg ccgacgggct ttaccgagct ggacgagatg accgccggtc
     1741 tccaaccgtc ggatctcatt atcgtggccg gacgcccgtc gatgggcaag acggcgctgg
     1801 cgctctccat ggcccagcat gcggcgattc acaaacgcca gacggttgcg gtgttcagcc
     1861 t
//
LOCUS       DBNGTT010000333         3434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2887395, whole genome shotgun sequence.
ACCESSION   DBNGTT010000333 DBNGTT010000000
VERSION     DBNGTT010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2887395"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16BC_07495"
     CDS             <1..417
                     /locus_tag="AC16BC_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3241502.1"
                     /translation="GAPGRTAALDLIHNGKRTTRTLKLGTVPGSKDAESASNDETGGA
                     KLNVTVAELGPEQKAELGVDHGVLVQDVKPGAAARAGVQPGDVILQVNGKRVESVSAL
                     RKGLDALAKGQPAALLIKRGDSTLFVAVEPNAEGVG"
     gene            1111..1275
                     /locus_tag="AC16BC_07500"
     CDS             1111..1275
                     /locus_tag="AC16BC_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241503.1"
                     /translation="MVDDWGSAVFAFAAITGGFGWMVLFGYELYTLAPPMTTEDHKEK
                     LPKTRHKKAA"
     gene            1486..2070
                     /locus_tag="AC16BC_07505"
     CDS             1486..2070
                     /locus_tag="AC16BC_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241504.1"
                     /translation="MFERLRELLHEAWRPVLGWHLAVASLGFAWIMYVHKTAPSGWVR
                     IIDDANLVFHEAGHPIFGLFFGSTLGLYGGTLGQLAIPFGIAVAFWVKREAVGFAVTG
                     FWFSENFLNIARYMADARAQVLPLVGGGEHDWTNIFSRWGVLASDTTIAGFAAGLGWL
                     GMIGAWGWLGWHWLASRADQQDEAVDIPSRWNAR"
     gene            2241..2573
                     /locus_tag="AC16BC_07510"
     CDS             2241..2573
                     /locus_tag="AC16BC_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HIR3241505.1"
                     /translation="MDTYYRPEDLGKFADMGKGNPELWEKFLAYYGAVFAEGALTERE
                     KALIALGVSHAVQCPYCIDAYTQACLEKGSNVEEMTEAVHVACAIRGGASLVHGVQMR
                     NVAHKLSM"
     gene            2584..>3434
                     /gene="arsS"
                     /locus_tag="AC16BC_07515"
     CDS             2584..>3434
                     /gene="arsS"
                     /locus_tag="AC16BC_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HIR3241506.1"
                     /translation="MPVTLPSLQARSNPLAETAEQRRIVAGANCQPFDDAIEQAGLFP
                     LTATNTRVFQINLGKLCNQTCRHCHVDAGPDRREMMSRDTLQACVDALAGTDIPVVDI
                     TGGAPELHPDFRWLVERARNLGRHVMDRCNLSVLLVASQTGLIEWLAQQRVEIVASLP
                     SANPGQTDSQRGEGVFDRSIAALRALNRVGYGVDGSGLTLNLVANPVGAFLPPKQGAI
                     EAQVRRELARKHSVVFNHLYTITNMPISRFLEFLIEGGNFEGYMERLANAFNPAAAKG
                     VMCRDIIS"
BASE COUNT          623 a          953 c         1164 g          694 t
ORIGIN      
        1 ggcgctccgg gccgcacagc cgctctcgac ctgattcata acggcaagcg caccacgcgt
       61 accctcaaac tcggcactgt cccgggatca aaagacgcgg agtcagcgtc caacgacgag
      121 acgggcggag caaagctcaa cgtgacggtc gcggaactgg gacccgagca aaaggccgaa
      181 ctcggcgttg accacggcgt gctggttcaa gacgtgaaac ccggcgccgc ggcgcgggcc
      241 ggcgtccagc ccggcgacgt gatcctgcag gtcaacggca aacgcgtgga gagcgtctcc
      301 gcgctgcgca aaggcttgga cgcattggcc aaaggtcagc ccgccgcgct gctcatcaaa
      361 cgcggcgaca gcaccctgtt cgtggcggtc gagccgaacg cggaaggcgt cggctagagc
      421 cgttggaggg agaaggaggt gagcgagatg ttcctgtttc tgatcgtcgc attgctgctc
      481 gcgacctgtc tgacatgggg agccgcagtg tgggcgtcct ttggtgataa gcaatgaccg
      541 aaggaaccgc gcgccgagaa ttccgcgaga aaaagagggg gacaggctac ttttggaaaa
      601 tccttaggcc cccctattgt aggcgaaaag tagcctgtcc cctttttgtc tcccgcggat
      661 cgcgagttcc ccgtggatcg gctcgatgcg ctggagctga agaaactcca atagattgtc
      721 gccaatcgcg cgtgcactgc gcgcacccac tcagtcgcgc ccttgcgatg cggccttcat
      781 caacgtgata atcgcttgct catccgtttc cttaggagcg ggtccctggt ttccaagggg
      841 cgctgtgatc agcgcaatac caaggcgaca tgcccaccca ttacatttca gaaacagacg
      901 actgagaacc gcgctactca atgtggtaat ttggcccaca gcgcggcgcg tgacggcgcc
      961 gttccgattt tttctaaccg attgatttcg taacatccac acggagacta tttctcggaa
     1021 agttgagggt ctttttcttg cttgcctttt ccggatcatg atttgtcgcc gcgcgccgtg
     1081 cacggccgca aacaccggaa ggaggtgcgt atggttgatg attggggctc ggcagttttt
     1141 gcgtttgccg ccatcacggg cgggtttggg tggatggtac tgttcggata cgaactctat
     1201 accctggctc ccccgatgac caccgaagac cacaaggaga aactccctaa gacaagacac
     1261 aagaaagccg catgagcctt gagctcagag ccccatcctc ggccgaggat ggggctctat
     1321 gcttatttgg ccagggtgga aggcgtcttg cgtagccgcc agtgagttcc gcacgttccg
     1381 tgtcggacga aacgtcgcac ggtctgtatt cttagggttt gagcggctgg gtgtcctacg
     1441 tcctgacgtc tcgggacgcg cgggcactgt tcagcgaggg atgggatgtt cgagcgatta
     1501 cgggaattgc ttcacgaggc ctggcggccg gtgctgggtt ggcacctagc ggtcgcatcg
     1561 ttgggttttg cgtggatcat gtatgtgcac aaaaccgcgc ctagcggctg ggtgcggatt
     1621 attgatgacg cgaatctggt gtttcacgaa gccgggcatc cgattttcgg cttgttcttc
     1681 ggatcgactc tcgggctgta cggcggcaca ttggggcagt tggcgattcc gttcgggatt
     1741 gcagtggcgt tttgggtgaa gcgcgaggcc gtgggctttg cggtgaccgg gttttggttc
     1801 tctgagaatt tcttgaacat tgcgcggtat atggctgatg ccagggcgca agtgctgccg
     1861 ttggtcggag gcggggagca cgactggacc aatatcttca gccggtgggg tgtgttggcg
     1921 tccgacacca cgatcgcggg gtttgccgcg ggcctcgggt ggctggggat gatcggcgcg
     1981 tgggggtggt tgggatggca ctggctggcg tcgcgcgctg atcagcagga cgaggcggtg
     2041 gatatcccgt ctcgatggaa cgcacggtag cgacccatca caatagatcg ttctcgccgc
     2101 gaccgagtgg gtcgcgtttt ccgctctgtt gatttcctgc tcatccatca cgcacccgcg
     2161 cgcatgacgt ttcgcggttg actggctgat cgcgttgata gtacggttac gcgtttcatg
     2221 ggaactcacg aggagaacgc atggacacgt attatcggcc ggaggatctg ggcaagttcg
     2281 ctgacatggg gaagggcaat cccgaactgt gggaaaaatt tctcgcgtac tacggcgcgg
     2341 tgtttgccga gggcgcgttg accgagcggg aaaaggcgtt gatcgcgttg ggcgtgtcgc
     2401 acgcggtgca gtgcccctat tgcatcgacg cgtataccca ggcgtgtctg gaaaagggct
     2461 cgaacgtcga ggagatgacc gaggcggtgc acgtggcgtg cgcgattcgg ggcggcgcgt
     2521 cgctggtgca cggggtccag atgcgcaacg tggcgcacaa gctctcgatg tgacggggcg
     2581 gccgtgccgg tgacgttgcc cagtttgcag gcccggtcca atcccttggc cgagaccgcg
     2641 gaacagcgcc ggatcgtggc aggtgcgaac tgtcagccat ttgatgacgc gatcgaacag
     2701 gccggcttgt ttccgctgac agccaccaac acccgggtct tccagatcaa cctcgggaag
     2761 ttgtgcaatc agacgtgccg ccactgccac gtggacgcgg ggccggatcg gcgcgagatg
     2821 atgagccgcg acacgctgca ggcgtgcgtg gacgcgttgg ccgggaccga tatcccggtg
     2881 gtggacatca cgggcggggc gccggagttg cacccggact ttcggtggct ggtcgaacgc
     2941 gcccggaacc tgggccggca cgtgatggat cggtgcaatc tcagcgtgct gctggtggcg
     3001 tcgcagaccg ggttgatcga gtggctcgcc caacagcgcg tggaaatcgt ggcgtctttg
     3061 ccgtcggcca atcccggcca gaccgattcc cagcgtggcg agggcgtgtt cgatcggtcg
     3121 atcgcggcgt tgcgcgcgct caaccgcgtg ggatacgggg tggatgggtc ggggctgaca
     3181 ttgaacctgg tggcgaaccc ggtaggggcg ttcctcccgc ccaagcaggg cgcgatcgaa
     3241 gcgcaggttc ggcgcgagtt ggcgcgaaag cacagcgtgg tcttcaacca tctctacacg
     3301 atcaccaaca tgccgatcag ccggtttctg gagttcttga tcgagggcgg aaacttcgag
     3361 ggctacatgg agcgcctggc caatgcgttc aacccggccg cggccaaggg cgtgatgtgc
     3421 cgggacatca tctc
//
LOCUS       DBNGTT010000334         3630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1866193, whole genome shotgun sequence.
ACCESSION   DBNGTT010000334 DBNGTT010000000
VERSION     DBNGTT010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1866193"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16BC_07520"
     CDS             complement(<1..230)
                     /locus_tag="AC16BC_07520"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR3241507.1"
                     /translation="MKAAVVVFPGSNCDRDCEHVLANVMGADVRMVWHRDAALPPVDL
                     VVIPGGFSYGDYLRTGALAACSPIMDAVRRHAG"
     gene            complement(241..621)
                     /locus_tag="AC16BC_07525"
     CDS             complement(241..621)
                     /locus_tag="AC16BC_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3241508.1"
                     /translation="MADYTFLVVEDSPTMRQLVSFSLKRIKGAKIVEATDGVDALKKL
                     SEQPFHLIVADINMPLMDGLKLLSIVRKEKAYSQIPVIIVTTEGADADRDKGLQLGAN
                     AYIPKPIQTNALLKAVRDLLKINE"
     gene            complement(679..1512)
                     /locus_tag="AC16BC_07530"
     CDS             complement(679..1512)
                     /locus_tag="AC16BC_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3241509.1"
                     /translation="MEKDKEPEIFRRADEFLGVFHKGIEFTNDLLKENERLRFAVAQM
                     AEQAKAAQKSTPSAELTRLRQQIDELAAEREAILNRYKLVEEENKDFASRYVEVEEEN
                     NNLANLYVASYQLHSTMDLKEVLRIVSEIILNLIGSDHFAILTRDEEEPVLHVAAAET
                     MDPKVEKIKLGEGMIGRVAQTGEPFVQEDLEHTVVSSPDRPIVCIPLKIHDTVIGVIA
                     IFDLFEQKRKRLTRVDHELFSMLAGHAATAIFSAMLYTRSQRSLSTIKDFLGLMKNKA
                     A"
     gene            complement(1587..2672)
                     /gene="cheB"
                     /locus_tag="AC16BC_07535"
     CDS             complement(1587..2672)
                     /gene="cheB"
                     /locus_tag="AC16BC_07535"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HIR3241510.1"
                     /translation="MPRPEPETVRVLVVDDSAFYRQALVMMLKEIPGVEVVGTASDGL
                     EAIQAVGKYRPTIITLDLEMPRMDGFGFLRWLMRTMPTPVLVISAASEAGNVFQALEL
                     GAADFLAKPTHLASWEILKTREDLHAKIRMMLAASPVRLSERAAKAASLRMTETVPVK
                     PALGAAERLKLVAIGSSTGGPAALQSIVASLPRDLPCAVAVAQHMPAGFTASFAERLN
                     RLSPWDVSEATPDQWCRTGQILICPGGHHLSFSRDRDLVKVVVERAGEEDRYVPSVDR
                     MMTSAAEVFGADLVGVILTGMGHDGRRGMARIKQQGGFTIAESQETAVVFGMPQEAIL
                     EGAVDIVAPLYAIAEQIGRHVTASIRR"
     gene            complement(2659..3522)
                     /locus_tag="AC16BC_07540"
     CDS             complement(2659..3522)
                     /locus_tag="AC16BC_07540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR3241511.1"
                     /translation="MIAIEQPQIPLTPDVFKLLRDMLHEHCGVWLDEQAKFLVEGRLQ
                     ETLRRLRFSGFKEFYYFLKYDARGADEFAVIVDLLTIHETYFFREDHQLYALQREILP
                     ELARRRTDRVLRIWSAGSSTGEEAYSVAMLLAGQPEYADWRIEIVASDISQRVLEVAR
                     RGVYPASAFRTTPPDYRSRFFHAEPDGFRIDDSIKRMVSFFSFNLADAERAALLPKMD
                     VILCRNVIIYFDLAVKKRVIEMFWNRLRPGGFLLLGHSESLINISTAFNLRHLRHDIV
                     YQKEERISAAA"
BASE COUNT          711 a         1202 c         1023 g          694 t
ORIGIN      
        1 cccgcgtgcc gccgcaccgc gtccatgatc ggcgaacagg ccgccaaggc ccccgttcgc
       61 aagtagtcgc cgtaggaaaa ccctccggga atgaccacca gatcgacggg agggagtgcg
      121 gcgtcccggt gccacaccat ccgcacgtcc gcgcccatga cgttcgccaa gacgtgttca
      181 caatctcggt cgcaattgga tccggggaac accacgaccg ccgccttcac gctaaggcac
      241 tcactcgttg attttcaaca aatcacgcac ggctttcaag agcgcgttcg tctggatcgg
      301 cttcgggata taggcgttgg cgccgagctg taagcccttg tcgcgatcag cgtccgcccc
      361 ttccgtagtc acgatgatca cgggaatctg gctatacgcc ttttccttgc ggacgatgct
      421 cagtaatttg agaccgtcca tcagtggcat gttgatgtcc gcgacgatca gatggaacgg
      481 ctgctcggac aatttcttca acgcgtcgac gccgtccgtc gcctcgacga tcttggcgcc
      541 cttgatccgc ttcaggctga aggagacgag ctggcgcatt gtaggcgaat cttcgacgac
      601 gagaaacgta tagtcagcca ttaccgtctc cccctctcac gtcgtatctc gggcaacccc
      661 gtcaaccgcc gccccacgtc aggccgcctt gttcttcatc aatcccagga agtccttgat
      721 ggtcgacagc gatcgttgcg acctcgtata cagcatcgcg ctgaaaatcg cggtcgcggc
      781 atggcccgcc aacatcgaga acaactcgtg atccacgcgc gtcagacgtt tgcgcttctg
      841 ttcaaagaga tcgaaaatcg cgatcacgcc gatgacggtg tcgtggattt tgagcgggat
      901 gcacacgatc ggacgatccg gagaggacac gaccgtgtgt tccagatcct cctgcacaaa
      961 cggctctccg gtctgcgcca cgcgcccgat catgccctcg ccgagcttga tcttttccac
     1021 cttcggatcc atggtttccg ccgccgccac gtgcagaacc ggctcctcct catcccgggt
     1081 gagaatggcg aagtggtcgg agccgatcaa gttcaaaatg atctcgctga cgatccgcag
     1141 aacctctttg agatccatcg tggaatggag ctgataactc gccacgtaca gattggcgag
     1201 attgttgttt tcctcttcca cttccacgta ccggctggca aaatccttgt tctcttcctc
     1261 gaccagcttg taccggttga gaatcgcctc tcgttcggcc gccaactcat cgatctgctg
     1321 ccggagcctg gtcaactcgg cggacggcgt ggacttctga gccgccttgg cctgttcggc
     1381 catctgcgcc accgcaaagc gcaatcgctc attttccttc agcagatcgt tggtgaactc
     1441 gatgcctttg tgaaagactc ccaggaactc atccgcccga cgaaagattt caggttcctt
     1501 gtctttctcc atcgccgtca tcctttcatg cgcccggagt atagcaccgg gctgttgggg
     1561 cccgcaagac gcgggcgaca acgcccttat cggcggatgc tcgccgtcac gtgcctgcca
     1621 atctgttccg caatcgcgta cagcggcgcc acaatatcaa ccgcgccctc caggatggcc
     1681 tcctgcggca tcccgaagac cacggcggtt tcctgcgact ccgcgatcgt gaacccgccc
     1741 tgctgcttga tccgcgccat gccccggcgt ccatcgtgac ccatgccggt caaaatcacc
     1801 ccgaccaagt ccgcgccgaa gacctccgcg gccgacgtca tcatgcgatc caccgacggc
     1861 acgtagcggt cttcctcccc ggctcgttcc accaccacct tgaccaggtc cctgtcccgc
     1921 gaaaaggaga gatgatgccc gcccggacaa atgagaatct ggcccgtccg gcaccattgg
     1981 tccggggtcg cctccgagac gtcccacggc gacaaccgat tgagtcgctc ggcaaacgac
     2041 gcggtgaacc cagccggcat atgttgcgcg acggccaccg cgcacggcaa gtccctgggc
     2101 agggaagcca cgatactttg cagggccgcc ggaccaccgg tcgacgaccc gatcgccacc
     2161 agcttgagtc gttccgccgc gccgagcgcc ggcttgacgg gaaccgtttc ggtcatccgc
     2221 agcgaggcgg ccttggcggc gcgctcgctg agtcgcacgg gggacgcggc gagcatcatc
     2281 cggatcttgg catgaagatc ctcccgcgtc ttgaggatct cccacgacgc gaggtgggtc
     2341 ggtttcgcga ggaagtcggc ggcgccgagc tcaagcgcct gaaagacgtt tccggcctcg
     2401 gacgcggcgc tgatcaccag caccggggtt ggcatcgttc gcatcaacca gcgcaggaac
     2461 ccgaagccgt ccattcgcgg catctcgaga tcgagagtga tgatcgtcgg gcgatacttt
     2521 cccaccgcct ggatcgcctc gagtccgtcc gaagcggtgc ccacgacctc gacgcccgga
     2581 atttccttga gcatcatgac cagcgcttga cgatagaacg ccgagtcatc gacgaccagg
     2641 acccgcacgg tctccggctc aggccgcggc actgattcgc tcctcttttt ggtacacgat
     2701 gtcgtgccga aggtgccgga gattaaacgc cgtgctgatg ttgatcaacg actccgagtg
     2761 ccccaagagc aagaatccgc cgggacggag ccggttccag aacatctcga tgacccgttt
     2821 cttcaccgcc agatcgaaat agatgatgac gtttcggcac aagatcacgt ccatcttggg
     2881 caggagggcc gcacgctcgg catcggcgag attaaacgag aagaaactca ccatccgttt
     2941 gatcgaatcg tcgatgcgga acccgtccgg ctcggcgtgg aaaaaacgcg aacgatagtc
     3001 aggaggcgtg gtccggaacg ccgaggccgg gtacacgccg cgccgcgcca cctccaacac
     3061 ccgctggctg atgtcgctcg ccacgatctc gatccgccaa tccgcgtatt ccggttggcc
     3121 ggccagcaac atcgcgacgg agtacgcctc ttctcccgtc gacgatccgg cgctccagat
     3181 ccgcaacacc cggtcagtcc gtcgacgcgc gagttccggg aggatttccc gctggagggc
     3241 gtacagctga tggtcctccc ggaaaaagta ggtctcatgg atggtcagga gatcgacgat
     3301 cacggcaaac tcatcggcac cccgcgcgtc atatttgagg aagtaataga actccttgaa
     3361 cccgctgaac cgaagtcgcc gcaacgtctc ctgaagccgc ccctcgacga gaaacttggc
     3421 ctgctcgtcc agccacacgc cgcagtgctc atgcagcatg tcgcggagga gcttgaagac
     3481 atcaggagtg agcggaatct gcggctgttc gatggcgatc acgcggtgtt cccggccttt
     3541 cggtttcgcc taggacgcct gatccaacac catcgcgagc ggatcgacgg catagtgagc
     3601 ggactcgcgc accagttggt caggatcacg
//
LOCUS       DBNGTT010000335         1624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_78127, whole genome shotgun sequence.
ACCESSION   DBNGTT010000335 DBNGTT010000000
VERSION     DBNGTT010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_78127"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="AC16BC_07545"
     CDS             complement(<1..387)
                     /locus_tag="AC16BC_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241512.1"
                     /translation="MPRTTPILKFASIALVIAMAASPALAVNLFTVNETSATGVAGSE
                     KLVPTFVSMADPTGIGGTFTQGGIYVPYMGTMTTCTSLIGTGSGDCSGWGSASIVAWA
                     NGLYYDPRFGTPFVPPSGDHHRTGYRM"
     gene            complement(773..1621)
                     /locus_tag="AC16BC_07550"
     CDS             complement(773..1621)
                     /locus_tag="AC16BC_07550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3241513.1"
                     /translation="MIDLYDEYTHAPLARRAFLARLARLAGGTAAAMALLPMLEASRA
                     RAALIAPNDPRIETESLFYPGATGSITAHLARLKGGMKLPAVIIIHENRGLNAHIEDV
                     TRRAAMEGFLALAPDLLSPAGGTPANEDTARDMIGKLETGQTVQNLVSAVAFLSKHPL
                     GNGAVGAVGFCWGGAMAGELAVHAPDLDAAVVYYGRQPHTEDVPKIHAPLLLHYAGLD
                     TRVNEGIPAFEDALKKANENYTLYRYEGANHAFNNDTSDARYNKQAAEQAWARTVAFL
                     KERLRP"
BASE COUNT          301 a          514 c          519 g          290 t
ORIGIN      
        1 catgcgatac ccggtccggt ggtggtcgcc ggacggcggc acgaacggcg tgccgaaccg
       61 cggatcatag tacagcccgt tcgcccacgc cacgatcgac gccgagcccc agcccgaaca
      121 atccccgctg ccggtcccga tcaacgacgt gcaggtggtc atggtgccca tgtacgggac
      181 gtagatcccg ccttgggtga acgtcccgcc gatcccggtg ggatcagcca tcgagacgaa
      241 cgtcggcacc aacttctcgc tgccggccac gccggttgcc gatgtttcat taacggtgaa
      301 gaggttgacg gccaaggcag gcgacgccgc catcgcaatc accaacgcga tgctcgcgaa
      361 tttcagtatc ggagttgtcc ttggcatcat gaggactcgc gtactgcgac accaggtctg
      421 gacaagagga tcttttcata taattgctgc aatggttgga ccagagtgga accgaaagga
      481 cgccggttga taaacaaccc gagcgaaaac aaataaatac caatacaaat ctcaagaatc
      541 gtgagcttgc tctccggtcg atacaaggac tgacgcgtag agaaaatggg aagggttttt
      601 cacgaaaatg gtcccgaaga aggactgaat cgggtcccgc gtcggcgaga caccgaccag
      661 cattcccgcg ttcgatgcac accgtcgccg gatgatccgg tgacgccgga accggccgag
      721 catcccgaag atattcctga cggacagtga gcagtccgcc cgccttcacg actcagggcc
      781 gcaaccgttc cttgaggaat gcgaccgttc tggcccacgc ctgctcggcg gcctgcttgt
      841 tgtagcgagc gtccgaggtg tcgttgttga acgcgtggtt ggcgccctcg taccggtaga
      901 gggtatagtt ctcgttggct ttcttcagcg cgtcttcgaa cgcggggatg ccttcgttga
      961 cgcgcgtgtc cagcccggcg tagtgcagca gcaacggcgc gtggatcttc gggacatcct
     1021 cggtgtgggg ctgccggccg tagtacacca cggccgcgtc caggtcgggc gcgtggaccg
     1081 cgagctcgcc cgccatcgcc ccgccccagc agaatcccac ggctccgacc gcgccgttgc
     1141 cgagcggatg cttggagaga aacgccacgg ccgacactag gttctgaacc gtctgtccgg
     1201 tctcgagttt gccgatcatg tcccgagccg tgtcttcgtt cgccggcgtc ccgcccgctg
     1261 gcgacaacag gtccggcgcg agcgcgagaa acccctccat tgccgcgcgc cgcgtcacgt
     1321 cttcgatgtg ggcgttgagg ccgcggtttt catggatgat gatcacggcc ggcagcttca
     1381 tcccgccctt gaggcgcgcc agatgggcgg tgatactgcc ggtggctccg ggatagaaca
     1441 ggctctccgt ctcgatccgc gggtcgttgg gagcaatcag cgcggcgcgg gcccgggagg
     1501 cctccagcat gggcagcaac gccatggcgg ccgcggtgcc tccggccaac ctggccagac
     1561 gcgccaggaa cgcgcggcgg gcgagcggcg cgtgcgtgta ctcgtcgtac aggtcgatga
     1621 tgtc
//
LOCUS       DBNGTT010000336         1669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_845460, whole genome shotgun sequence.
ACCESSION   DBNGTT010000336 DBNGTT010000000
VERSION     DBNGTT010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_845460"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="AC16BC_07555"
     CDS             complement(<1..851)
                     /locus_tag="AC16BC_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type ATPase actuator domain-containing
                     protein"
                     /protein_id="HIR3241514.1"
                     /translation="MPTGAHAARHRVAESRPAPTTGPDHWHRMEADDVLHALKTSRKG
                     LSSVEAEHRLTAHGLNALPAGGGTKLWAAIVSQLEDVMIVVLLGAAVIAWAAGDMADA
                     ALIAAVVVINLALGLGQHLRAERAVAALRELEEPVATVRRDGRVLNVPSVRLVPGDLV
                     LLEAGDRVPADGRLLEAVQLRVDESSLTGESVPADKHVRRLQAVAPPLGDRTNMAYMG
                     TTVAAGRGAAVVTETGLSAELGRIAGLLQTVEEPPTPLQRRLNELGKRLALATVILTS
                     LVFAVGV"
     gene            1066..1521
                     /locus_tag="AC16BC_07560"
     CDS             1066..1521
                     /locus_tag="AC16BC_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3241515.1"
                     /translation="MSALTRWDPFRELDDLQNRLSTLFGRAPVRKEGEREEKMTLVDW
                     APLVDVIEQEKEYVIKAELPEIKREDVKVTVQDDVITIAGERTREKEEKGKKFHRIER
                     AYGSFSRSFTLPEDADPAKVSAEFKNGVLNVHLAKSEKAKPKTVEVTVT"
BASE COUNT          303 a          568 c          543 g          255 t
ORIGIN      
        1 cacaccccca ccgcgaacac gagcgaggtg aggatcacgg tggccagcgc cagacgcttc
       61 ccgagctcgt tcagacggcg ctgcaacggc gtgggcggct cttccacggt ctggagcagc
      121 cccgcgatcc gtcccaactc cgcggacagg ccggtttcgg tcacgaccgc cgcgccgcgg
      181 cccgccgcca ccgtggtgcc catgtacgcc atgttggtgc ggtcgcccaa tggcggcgcc
      241 acggcctgga gtctccggac gtgcttgtcc gcgggcacgg attcgccggt caacgaggat
      301 tcgtcgaccc ggagctggac cgcttcgagg agccgtccgt ccgccgggac gcgatccccg
      361 gcctcgagca gcaccaggtc tcccggcacc aggcggaccg acggcacgtt cagcacgcgc
      421 ccgtcgcgcc ggaccgtggc gaccggctct tcgagctcgc gaagcgccgc caccgcgcgc
      481 tccgcgcgaa ggtgctggcc caggccgagc gcgaggttga tcaccaccac cgccgcgatc
      541 aacgcggcgt cagccatgtc gcccgcggcc cacgcgatca cggccgcccc gagcaggacc
      601 acgatcatca cgtcctcgag ctggctcacg atcgccgccc acagcttggt gccgccgccg
      661 gccggcaacg cgttcagccc gtgcgcggtg agccggtgtt ccgcctccac gctcgagagg
      721 cccttgcgcg acgtcttgag cgcgtggagc acgtcgtccg cctccatccg gtgccagtgg
      781 tcaggcccgg tggtgggcgc agggcggctc tcggccacgc gatgtcgcgc cgcatgagct
      841 ccggttggca tggccctagc ctacaggtct gcttcccgag aatccacccc tccaaacgga
      901 tgagcgcctc ccgccggaca acctacccgt ttgcgcgatc gccatcatcc gtttgcgtcg
      961 atgccccctc ctttgcgggg gttgtcccgc gccccgcgga tttgataggt tggtggcgct
     1021 gaaatggggt attcacttca actttcattc gggaggagag tcgaaatgag tgcactgacc
     1081 cgctgggatc cgttccggga actcgacgat ctgcaaaacc gtttgtccac attgttcgga
     1141 cgggcgccgg tccgcaaaga gggcgagcgc gaggaaaaga tgaccttggt ggactgggcg
     1201 ccgctggtcg acgtgatcga acaagagaag gagtacgtca tcaaggccga gttgcccgag
     1261 atcaaacggg aggacgtgaa ggtcacggtg caggacgacg tgatcaccat cgcaggcgaa
     1321 cgcacgcgcg aaaaagagga aaagggcaag aagttccacc gcatcgaacg cgcctacggc
     1381 tccttctcac gcagcttcac gctcccggaa gacgcggacc ccgcgaaggt cagcgcggag
     1441 ttcaagaacg gcgtgctgaa cgtgcatctg gccaaatccg aaaaagccaa acccaagacc
     1501 gtcgaagtca cggtgacgta ggaatggacg tagcctcgtg cgcctcactg tctttctttc
     1561 gaaactcatc gcagatgaga gcctgttcag gagcggtcag atgcaaggcg ccgcgagcac
     1621 cggagcggag cgtacgaggt cggtacgtga gcaccggatg cgcagcggc
//
LOCUS       DBNGTT010000337         2560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_589604, whole genome shotgun sequence.
ACCESSION   DBNGTT010000337 DBNGTT010000000
VERSION     DBNGTT010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_589604"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1328
                     /locus_tag="AC16BC_07565"
     CDS             45..1328
                     /locus_tag="AC16BC_07565"
                     /inference="COORDINATES: protein motif:HMM:NF023330.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HIR3241516.1"
                     /translation="MIPFDRLLSVPAHIHHAAYLMKDPPSESRAARDAFARTLSNLGV
                     GPDRSVINDRFGYGVLEHAAGDRLVIRWQLHTEYYTYQLWYLPADPARRLAFGPLEWP
                     GAPPVCSAFGETVTTVDLIVTDRATEVAPDFGLSADHTWFGGRVLGEPVLLFTDFAPD
                     AHGRRRYLLHGHDRKVLAQVTPFAADCVAKIENYYHLILAPLPGFSAAMDQVFRLEKR
                     QLADRDAITRGLAAADPAQLQAWLTQLTEALADVSRLGEAIRYNLASAGPYDSILRTT
                     VEDLREQPLERLESLGAHVLRRIRGIADGYQRLIQRIEALEQSFEGMVSIIRTRIDLA
                     MEQQNLTILSSVDRTTKIQVRLQHMVESLSIVVQAYYLTGLGSYVFKAMEHYGWLGDA
                     TTATALFIPAAFALAFFLTWQAKRLLTRHTEGSGS"
     gene            1325..1852
                     /locus_tag="AC16BC_07570"
     CDS             1325..1852
                     /locus_tag="AC16BC_07570"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3241517.1"
                     /translation="MIPIGESVPDFSLPGVRGERDARWRLSDARGRWVVLFFYPADFT
                     FVCPTEVVGFHRHLDAFRKLGVDILGVSVDATDSHRAWAKELGGIDYPLLADTDRAVS
                     AAFGVLDRQAGVARRVTFVIDPAGVVAYVVASHHNVGRSVEETLRVVAALQTGRLCPA
                     EWQPGEPTSDLGPKY"
     gene            2057..>2560
                     /locus_tag="AC16BC_07575"
     CDS             2057..>2560
                     /locus_tag="AC16BC_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3241518.1"
                     /translation="MTTLDDKRRRIEQRGRVREFVFGIQDGLISNLGLVTGIQGATAD
                     LKIVLLGGITAAVSGAISMAAGSYLSSKAEKEIFDAEIRAETDRLAGEPYLAQEAVLE
                     SLKTEGLPRENAYRVVRLMQPRPDILLQTYHEKVLGLGRAEINRPIQAALVMAVSVTV
                     GSVIPLVP"
BASE COUNT          406 a          795 c          877 g          482 t
ORIGIN      
        1 cgggttcggc aatctgagtc cgttttgacc gccggcgttg tccaatgatc ccgtttgatc
       61 gtctcctgtc cgttccggcc catatccacc acgccgctta tctaatgaag gacccgccgt
      121 ccgagagccg ggccgctcgc gacgcgttcg cgcgaacgtt gtcgaacctc ggcgtcggac
      181 cggatcgctc ggtcatcaac gatcgcttcg ggtacggggt ccttgagcac gccgcgggcg
      241 atcgcctggt gatccgttgg cagctccaca ccgagtacta cacgtatcaa ctctggtact
      301 tgcccgctga tccggcgcgc cgcttggcgt tcggccccct ggagtggccg ggcgctccgc
      361 ccgtgtgctc tgcgttcggg gagaccgtca ccacggttga tttgatcgtg accgatcggg
      421 ccaccgaggt cgcgcccgac ttcgggctct ccgcggacca cacgtggttc ggcggccggg
      481 tgttgggcga gccagtgctg ctgttcacgg acttcgcgcc ggatgcgcac ggccggcgac
      541 ggtacttgtt gcacggccac gatcgcaagg tgctcgcgca ggtgacgccg tttgcggccg
      601 actgcgtcgc gaagatcgaa aattactacc acctgattct ggcgccgttg cccggttttt
      661 cggcggccat ggatcaggtc ttccggctgg aaaagcgcca actcgccgac cgcgacgcga
      721 tcacgcgcgg actggctgcc gcggacccgg cgcagctgca ggcgtggttg acgcagttga
      781 ccgaagcctt ggccgatgtg agccggctgg gcgaagcgat ccgctacaac ttggcgtccg
      841 cgggacccta cgactcgatc ctccggacca cggtcgagga cctgcgcgag caaccgttgg
      901 aacggctgga gtcgttgggc gcgcacgtgc tccgtcgcat ccggggtatt gccgacgggt
      961 accagcgcct gatccagcgc atcgaggcgt tggagcagtc cttcgagggc atggtgtcga
     1021 tcatccgcac acggatcgat ctggccatgg aacaacaaaa tctcacgatc ttgtcgagcg
     1081 tggaccgcac cacgaagatc caagtccgtc tgcaacacat ggtggagtcg ctctcgatcg
     1141 tggtccaggc gtactacctg acggggttgg gaagttatgt gttcaaagcg atggaacatt
     1201 acggctggtt gggcgacgcc accaccgcca cggcgctgtt cattcccgcg gcgttcgccc
     1261 tggccttttt cctgacgtgg caggccaaac gtttgctcac gcgccacacg gagggatcag
     1321 gatcatgatt ccgatcggcg agtcggtccc ggatttttcc ctgccgggag tgcggggcga
     1381 gcgggacgcg cgatggcggt tgtcggacgc gcgcggccgg tgggtggtgc tgtttttcta
     1441 ccccgcggac ttcacgttcg tctgcccgac cgaggtcgtg ggctttcacc gccacctgga
     1501 cgcgtttcgc aagctcgggg tggacattct gggcgtgagc gtggacgcga ccgacagcca
     1561 ccgcgcgtgg gcgaaggaac tcgggggcat cgactacccg ttgctcgcgg acacggatcg
     1621 cgcggtctcc gccgcgttcg gcgtactgga tcggcaggcg ggcgtggcgc gccgcgtcac
     1681 gttcgtcatt gatccggccg gcgtggtggc gtacgtggtg gcgagccatc acaacgtggg
     1741 gcgaagcgtg gaagagacgc tgcgggtcgt ggccgcgctg cagaccggac ggttgtgtcc
     1801 ggcggagtgg cagccgggag agcccacgtc cgacctcgga ccaaagtatt agaagcccgt
     1861 cgataagcgg ccatctgcgg cgttgtcgct gcgcgtccgc tcctcacgta cgacccagta
     1921 cgctgcggtg cgggcgctcg ctccgccttg cgacccaccg ccacaaattc aaatggcgga
     1981 agtccccgaa ggggggcatc tggcgcgctc ctcaacgggc ttcatgcgcc tgaattcgtg
     2041 gcgcatgttg gggacaatga cgacgcttga cgacaaacgc agacgcatcg agcagcgcgg
     2101 acgggtgcgg gagttcgtgt tcgggattca ggacggtctc atcagcaacc tcggattggt
     2161 caccgggatc cagggcgcga cggcggattt gaagatcgtg ttgctcggcg gcattaccgc
     2221 ggcggtgtcg ggcgcgattt ccatggcggc ggggtcctac ctgtcctcga aggcggagaa
     2281 agaaattttc gacgctgaga ttcgcgccga gacagaccgt ctggcgggcg agccgtacct
     2341 ggcgcaagaa gccgtgctgg aatcgctcaa gaccgagggc cttccgcggg agaacgcgta
     2401 ccgggtggtg cggctgatgc agccgcgtcc cgacatcctg ttgcagacct atcatgaaaa
     2461 ggtcttgggg cttgggcgcg cggagatcaa ccggccgatt caagccgcgc tggtgatggc
     2521 cgtctcggtc accgtggggt cggtcatccc gcttgtgccc
//
LOCUS       DBNGTT010000338         5902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1612340, whole genome shotgun sequence.
ACCESSION   DBNGTT010000338 DBNGTT010000000
VERSION     DBNGTT010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1612340"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="AC16BC_07580"
     CDS             complement(<1..831)
                     /locus_tag="AC16BC_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3241519.1"
                     /translation="MAKPVTRVVGGLVLVAVLALLALSFLPQAVPVDAAPVTQGRFEQ
                     TVEEDGKTRVRERYVVSAPLAGRVLRIELKEGDTVAQRGLLAVMLPAAPALLDVRTEQ
                     ELKERLAAAEDEWRRTKAVVQRSKAALKQARIDFNRSRELAREHLVPPAQLEQSELAV
                     TIQTKELEAAEFQDNVAGHQVDLARAALLQARRGIDPKAAPDRLEIRSPVAGRVLRVL
                     QESEAVVALGAPLLEIADPSDLEVVVDVLTTDAVRITPGMRVRIERYGAGQPLEGRVR
                     L"
     gene            complement(831..1091)
                     /locus_tag="AC16BC_07585"
     CDS             complement(831..1091)
                     /locus_tag="AC16BC_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241520.1"
                     /translation="MSWFLLGELAIELVAAIPLGLALGYALAATLINLMHDETFAMPM
                     VIEPRTYAFAALAILIAGIASALVVRHRVDHLDLVAVLKTRE"
     gene            complement(1092..3191)
                     /locus_tag="AC16BC_07590"
     CDS             complement(1092..3191)
                     /locus_tag="AC16BC_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241521.1"
                     /translation="MKALDKKLLRDLWHTRSQVFTIALVVAGGMGAFIASFCTYDSLQ
                     WSRHVYYEVARFGEVFAGLKRAPAPLEARIAELPGVVEAETTVVFDVTLDLPGVKEPV
                     IGRMIGLPNGAQPRMNRLAIRRGRFIEPGRHREVLVSEGFANARHIGPGDRLAAILNG
                     KREELDIVGVVLSPEYVFATRGSSLPDDRSFGVFWIDRERLASAFDMEGAFNSVVARL
                     APGAGESAVIADLDRLLEPYGAFGAYGRGDQISNRILSQEISQQRVWGTVLPAIFMAV
                     AAFLVNVVLSRHVATQREAIAALKALGYSNVRIGAHYLAFVSVIVVIGSGLGLGVGWW
                     LGRGMTRMYTEFFHFPHLIFRIQPWVPLLGAGISFVAAVGGALNTVRVVAALAPAEAM
                     RPPSPAQYRRMLVERIGMERWLSPAARMVIRTLERRPFRAAFTSFGVACSVAIIISGT
                     FWRDVVDYMIDVQFNAVEREDASVVLTDPREARVRHEIERLPGVSQVEGAREVPVRLR
                     NGHRTYRTGIIGLSQGAQLRRLLDQDLVEVPLPGDGMLLTDRLATRLGLAPGDTVVVE
                     SLEGARITPEVIVVGVVADVFGMVGYMEINALNRLMGEGPSVSSIAVSVDRDRADELY
                     SRLKERPRVATVTVKASALRTFEETSARNILFFTTVITVFAAAIAIGVVYNSARIALA
                     ERAWELACLRVLGFTRR"
     gene            complement(3188..3847)
                     /locus_tag="AC16BC_07595"
     CDS             complement(3188..3847)
                     /locus_tag="AC16BC_07595"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3241522.1"
                     /translation="MGEVEVHALRGVDLDIYQGEFVVLLGPSGSGKSTLLNILGGLDT
                     PSSGEVRWRDHELTRADERALTTYRRQHVGFVFQFYNLISSLTVRENVALVTDIADQP
                     MHPDEALRWVGLEERLNHFPSQLSGGEQQRVAIARAIAKRPEVLLCDEPTGALDYQTG
                     KVVLEAIARINAELGTTAVVITHNVAISGMADRVMHLADGRISATEKNPRKLTPSELS
                     W"
     gene            complement(4123..4662)
                     /locus_tag="AC16BC_07600"
     CDS             complement(4123..4662)
                     /locus_tag="AC16BC_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241523.1"
                     /translation="MLLAVVGAFVTLPELWREPSLGTAASAAILTAAAVWLSWIRISG
                     LWHCIAARPRVVPYFQTRVGANDEPCGAFEHGFGVANRMAALDAAALERGVAQPSEFG
                     FSDEPKRWWDARDGLRTLDAIAGSVADSDELDQDLAALRSALEKAAEKEIPFALLVRL
                     GPDTFVSLYEMDTRKGSFW"
     gene            complement(5231..5587)
                     /locus_tag="AC16BC_07605"
     CDS             complement(5231..5587)
                     /locus_tag="AC16BC_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3241524.1"
                     /translation="MITAMAFTVYPVRDMARSRRFYENVLGLTAGYVFGDQWVEYDVG
                     GSTFAVTTTDMGHPPGAKGAVVAFETTDLDAFVKRLKEQSVPFVVETFATPVCRMAVI
                     EDPDENHVTIHQRQVR"
     gene            complement(5602..>5902)
                     /locus_tag="AC16BC_07610"
     CDS             complement(5602..>5902)
                     /locus_tag="AC16BC_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3241525.1"
                     /translation="RVFPGLAKDVVFQDVSSPMTHTRYTHSTGGTAYGIALIPSQSLF
                     RRVGHATDVPGLYLCGASTYTGHGITGVMWSGVLAASRIAGAGVLGDVTRPSRRS"
BASE COUNT         1069 a         2089 c         1800 g          944 t
ORIGIN      
        1 caggcgcacg cggccttcca gcggttggcc ggccccgtag cgctcgatgc gcacgcgcat
       61 gccgggcgtg atccgcaccg cgtccgtggt caacacgtcg accacgactt ccaggtcgga
      121 cgggtccgcg atctccagca gcggggcgcc gagcgccacc accgcctcgc tctcctgcaa
      181 cacgcgcagc acgcgcccgg ccacgggcga gcggatctcc aggcgatccg gcgcggcctt
      241 gggatcgatg ccgcgccggg cttgcagcaa cgcggcgcgc gccagatcca cctggtgccc
      301 ggccacgttg tcctggaact ccgcggcctc gagctccttg gtttgaatgg tcaccgcgag
      361 ttcggattgt tcgagttggg ccggaggaac caggtgttcc ctggccagtt cgcggctccg
      421 attgaagtcg atcctggcct gcttcagagc cgccttggag cgctggacca cggctttggt
      481 ccttcgccac tcgtcctcgg ccgcggcgag ccgctccttc agctcttgct cggtgcgcac
      541 gtccagcaag gcgggggccg cggggagcat cacggccagg agcccgcgtt gcgcgaccgt
      601 gtcgccctcc ttgagctcga tgcgcaacac ccggcccgcc aacggggcgg agaccacgta
      661 gcgctcgcgc acccgggtct tgccgtcttc ttccacggtc tgctcgaagc gtccctgcgt
      721 gaccggcgcc gcgtcgaccg ggaccgcttg cgggagaaaa ctcagggcca gcagggccag
      781 gacggcgacc agcacgaggc cgcccaccac gcgtgtcacg ggtttcgcca tcattccctc
      841 gtcttcaaca ccgccaccag atcaaggtga tccacgcggt ggcgcaccac caaggcgctg
      901 gcgatccccg cgatcaggat cgccagcgcc gcgaacgcgt atgtgcgggg ctcgatcacc
      961 atgggcatgg cgaacgtctc gtcgtgcatg agattgatca aggtcgcggc caacgcgtac
     1021 ccgagcgcca agcccaatgg aatggccgcg accagttcga tcgccagctc ccccaacagg
     1081 aaccacgaca cctagcgccg cgtgaatccc aggacgcgaa gacacgccag ttcccaggcg
     1141 cgctccgcga gcgcgatgcg agcgctgtta tacaccactc cgatcgcgat tgccgcggca
     1201 aacaccgtga tcacggtcgt aaaaaagagg atgttccggg ccgaggtctc ctcgaaggtt
     1261 cggagcgcgg acgccttcac ggtcacggtc gcgacccggg gccgctcctt gagccggctg
     1321 taaagctcgt ccgcccggtc ccggtccacc gacaccgcga tcgacgagac cgaagggcct
     1381 tcccccatca ggcgattgag ggcattgatc tccatgtacc ccaccatgcc gaacacgtcg
     1441 gccaccaccc cgaccacgat cacctcgggc gtgatcctgg cgccctccaa ggattcgacc
     1501 accacggtgt cccccggcgc aaggcccaga cgcgtggcca ggcgatcggt cagcaacatc
     1561 ccgtcgccgg gcaagggcac ctcgaccaga tcctgatcga gcaggcgccg caattgcgcg
     1621 ccttgcgaca gcccgatgat cccggtgcga tacgtccggt ggccgttgcg cagccggacc
     1681 ggcacttccc gcgccccttc cacctgggac acgccgggga ggcgctcgat ctcgtggcgc
     1741 acccgcgcct cccgcgggtc ggtcagcacc acgctcgcgt cctcccgctc cacggcgttg
     1801 aactggacgt cgatcatata gtcgactacg tcccgccaga acgtcccgga gatgatgatc
     1861 gccaccgagc aggcgacccc gaacgaggtg aacgcggcgc gaaacggccg gcgctccagg
     1921 gtgcggatga ccatccgcgc cgcgggcgac agccaccgct ccatgccgat ccgttcgacc
     1981 agcatccgcc ggtactgcgc gggcgatggc ggccgcatgg cttcggccgg cgcgagcgcc
     2041 gcgaccaccc ggaccgtgtt gagcgcgccc cccaccgcgg cgacaaaact gatccccgcg
     2101 ccgagcagcg gcacccacgg ctggatgcgg aagatcaggt gcgggaaatg gaagaactcg
     2161 gtgtacatcc gcgtcatgcc gcggcccagc caccacccga cgcccagccc gaggccggag
     2221 ccgatcacca cgatcaccga gacgaacgcg agatagtgcg cgccgatccg gacgttggag
     2281 tacccgagcg ccttcaacgc ggcaatggcc tcgcgttggg tggccacgtg ccggctcagc
     2341 accacgttga ccaggaacgc ggccacggcc atgaagatcg cgggcagcac ggtgccccag
     2401 acccgctgct ggctgatctc ctggctcaag atgcggttgg agatctggtc gccccggccg
     2461 taggcgccga acgcgccgta gggttcgagc aagcggtcga ggtccgcgat gaccgcggat
     2521 tcgccggcgc ccggcgccag acgcgccacc acggagttga acgcgccctc catgtcgaag
     2581 gccgaggcca gccgctcgcg gtcgatccag aacacgccga acgaacggtc gtccggcagc
     2641 gagctgccgc gggtcgcgaa cacgtactcc ggcgacagca ccaccccgac gatgtccagc
     2701 tcctcgcgct tgccgttgag gatcgcggcc agacgatcgc cgggcccgat gtgtctggcg
     2761 ttggcgaatc cctcggagac gaggacctcg cgatgacggc ccggctcgat gaaccgaccg
     2821 cgccggatcg ccagccggtt catgcgcggc tgcgccccgt ttggcaaccc gatcatccgg
     2881 ccgatcacgg gctccttcac cccggggaga tcgagcgtga cgtcgaagac cacggtggtc
     2941 tccgcctcga ccacgcccgg cagctcggcg attctcgcct ccaggggcgc gggcgcgcgt
     3001 ttgagtccgg caaagacttc tccgaatcgc gcgacctcgt agtacacgtg gcgggaccac
     3061 tgcaacgaat cgtacgtgca gaacgaggcg atgaaggcgc ccatcccgcc cgccaccacc
     3121 aatgcgatcg tgaacacctg gctccgggtg tgccagagat cgcgcaggag tttcttgtcg
     3181 agcgccttca ccagctcagc tccgacgggg tgagtttgcg cggattcttc tcggtcgcgg
     3241 agatgcggcc gtccgccagg tgcatcaccc ggtccgccat acccgagatc gcgacgttgt
     3301 gcgtaatgac cacggccgtg gttcccagct cggcgttgat ccgcgcgatg gcttccaaca
     3361 ccacctttcc ggtctggtaa tccaacgcgc cggtcggctc atcgcacagg agcacctccg
     3421 gacgtttggc gatggcgcgg gcaatggcca cgcgctgctg ctccccgccc gagagctggg
     3481 aggggaagtg attgagtcgc tcctccaagc ccacccagcg cagcgcttca tccgggtgca
     3541 tgggctgatc cgcgatgtcc gtgaccaggg ccacgttttc gcgcacggtc agactggaga
     3601 tgaggttgta gaattgaaac acgaacccca cgtgctgccg gcgataggtg gtcagcgcgc
     3661 gctcgtccgc cctcgtcagt tcgtgatccc gccagcgcac ctccccgctg gagggcgtgt
     3721 cgagcccgcc gaggatgttg agcagcgtgg atttaccgct ccccgagggc cccaagagca
     3781 ccacgaattc gccctggtag atgtcgagat ccacgccgcg caatgcatgg acctcgacct
     3841 cgcccatgcg atagaccttg gtgaggccgc gcgcggagaa gacgctggtt ggcgacggat
     3901 tgtcgatcgg gattgtccct ttcttcagga atgcagcggc aaccggaccg ttgcgccgat
     3961 tccgtgttgc gcgccgacca cctgtatcgc ggcggcatcg tgatcaagat ggcgcgtcaa
     4021 accgtctgga cgcttctcaa tccacgcgca atgaatgtcc caaaccagcg cggcgacgga
     4081 ttgaaccgcc tctgtgttgg gcgccgacca ggcgacgcgc tttcaccaga atgatccttt
     4141 gcgggtgtcc atctcatata gggagacgaa cgtatcgggt cccaacctca ccagcagcgc
     4201 aaacggtatc tctttctcgg cagccttctc cagcgcagac cgcaacgccg ccaagtcctg
     4261 atcaagctcg tccgagtctg caaccgaacc agcgatcgca tcgagcgtcc gcagcccgtc
     4321 ccgagcgtcc caccagcgct tgggctcgtc cgagaatccg aattcggacg gctgcgccac
     4381 tcccctctcc aacgctgccg catcaagggc agccatcctg ttggcaaccc cgaagccatg
     4441 ctcgaatgct ccgcaaggtt cgtcgttagc cccaacgcgt gtctggaaat atggcacgac
     4501 ccgaggccgc gcggcgatgc agtgccaaag accggagatc ctgatccatg aaagccacac
     4561 ggccgcggca gtgagtatcg cggcggaagc tgccgtcccg agggacggct ctcgccacaa
     4621 ttccgggagt gtcacaaatg caccgacgac cgcgaggagc atgccgaagt tcagcagcag
     4681 acaaatcgga tgaatcaggt ttgcgacgag acaatcctcc ctaagtctcc tgacttcgcg
     4741 ctgcagagaa ccgcctccac cactcaacgt tcagacactc tccctcacgt gcgcctaccg
     4801 gctggcggct cagcggcgag cacgcagcgc gagtccgctc agccgcgggt tagacggccc
     4861 ctgatcgctc ccgacgcaag aacgattaac tttcggaggc catacgacta aaaatcgaaa
     4921 aatcggggtc agagtactat ttcggttcgc tgacgctcgc aaagatcgaa aaagcacgct
     4981 gaccctaatt tccgtggccg ctggtgcctc cagccgattg gaccgtccgt cggctcgcca
     5041 cacctctcgc cgacgaggaa atcacccagt tacgcacgtg cgtgaaccga caggccccgt
     5101 ttggtgctcc cgactggatc gctcgcatgg ctgccacgct cggcctgcaa tctacgctgc
     5161 gcactctcgg aagacctcac aaactttccc cggaaaagta gcctgtcccc ttttttctgt
     5221 ggggcggcct tcagcgaacc tgtcgctggt gtatcgtgac gtggttctcg tccggatctt
     5281 caatcacggc cattcgacag accggcgttg caaacgtctc gacgacaaag ggcaccgact
     5341 gttccttgag tcgtttcacg aacgcatcga gatccgtggt ctcaaacgcg accaccgcgc
     5401 ccttagcccc tggaggatgt cccatgtccg tggtggtaac ggcaaacgtc gagcctccga
     5461 catcatactc gacccactga tctccaaaca cgtaccccgc cgtgagtccc aacacgttct
     5521 cataaaatcg cctggaacgc gccatgtccc tcaccgggta caccgtgaac gccattgcgg
     5581 taatcatccc cggtcctttc ttcacgagcg tcggctcggt cgcgtgacat cccccaacac
     5641 gcccgcgccc gcgattcgcg acgcggccaa caccccggac cacatcaccc ccgtgatccc
     5701 gtgtccggtg tacgtgctcg cgccgcacag gtacaggccg ggaacgtccg tggcgtgtcc
     5761 gacgcgacga aacagcgact gcgacgggat caacgcgatg ccgtacgcgg tgccgccggt
     5821 ggagtgcgtg taccgggtgt gcgtcatggg gctcgacacg tcctggaaga ccacgtcctt
     5881 ggcgagcccg ggaaacacgc gg
//
LOCUS       DBNGTT010000339         4793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_718728, whole genome shotgun sequence.
ACCESSION   DBNGTT010000339 DBNGTT010000000
VERSION     DBNGTT010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_718728"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..612
                     /locus_tag="AC16BC_07615"
     CDS             118..612
                     /locus_tag="AC16BC_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR3241526.1"
                     /translation="MGRAGDNDETPSPRHPVSPSLRCSDSGPHWEHFPHQSDIGVRGV
                     GASPAEAFEQAALALIAVMSDPATVEPREAVAVTCDAPDLELLFVEWLDRLIYEVATR
                     HMLFGRFTVRIDGLRLTGQAWGEAIDPARHPCVVEVKGATLTSLGVAQGADGRWTAQC
                     VVDV"
     gene            620..2050
                     /locus_tag="AC16BC_07620"
     CDS             620..2050
                     /locus_tag="AC16BC_07620"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR3241527.1"
                     /translation="MDLGRLIRVSDTEWHIERHGAMRVPGVIYATESLIREMDDKVYE
                     QVTNVATLPGIEVGSFAMPDAHWGYGFPIGGVAAFNPTEGGVISAGGVGFDISCGVRA
                     LLTGLRREQVEPVKMALADALFRAVPAGVGSTGLIRLSAAEMDAMLRGGAQWAVAQGY
                     GSQADLDRIESGGRTPDADPGEVSEYAKRRQRDEMGTLGSGNHYLEVQHVARVHDAAL
                     AEAFGLHEGDVIVSIHCGSRGLGHQIGTEFLKRMATEAPQHGIHLPDRELACAPIESA
                     LGRAYLGAMRAAMNCAMANRQIITHLVRQAVAGVLPDARLPLLYDVSHNTCKVEEHPV
                     DGRLTRLYIHRKGATRAFGPGHPDIPSALRAVGQPVLIGGTMGTASYILCGTRAGMSR
                     SFGSACHGAGRSMSRHQATRQWRGRDVVDDLARRGIIVRSPSLRGVAEEAPGAYKDVS
                     EVVGAADRAGLARTVARLEPLVCIKG"
     gene            2127..2606
                     /locus_tag="AC16BC_07625"
     CDS             2127..2606
                     /locus_tag="AC16BC_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR3241528.1"
                     /translation="MARGRRFWLMKSEPEVFSIEDLRRADGQTTCWDGVRNYQARNFL
                     RDGIKTGDGVLYYHSNAQPPGIAGEAVVVRDGYPDFTAFDPDDPHYDTKSDPNRPTWY
                     MVDVRFVRACKALIALDRLKAISTLRSMLVLRRGMRLSVQPVTTKEWDAIMALPEWG"
     gene            2608..3345
                     /locus_tag="AC16BC_07630"
     CDS             2608..3345
                     /locus_tag="AC16BC_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR3241529.1"
                     /translation="MTGRPSSWFAKWGFLTALIGAAACAFAGWGTRAELWSFGAGLRV
                     LRLAAVGALFGSALSLIGLIATRGGKKRGRFSAILGVVLGLAAAGLPAAQDMKARTLP
                     AIHDITTDLENPPAFSAVVPLRRGAPNGVEYEGASVADQQRRAYPDIQPLSLVVPANQ
                     AFDRALEAAREMGWTIVDANPAHGRIEATATTFWFGFKDDVVVRVSATDSGSRIDVRS
                     LSRVGRSDIGANARRIRCYLDKLSAVG"
     gene            complement(3342..>4793)
                     /gene="mutS"
                     /locus_tag="AC16BC_07635"
     CDS             complement(3342..>4793)
                     /gene="mutS"
                     /locus_tag="AC16BC_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR3241530.1"
                     /translation="KILAGGAEPRVRALGEGWEDFGQLIDRLTTALVPTPPPSARDGG
                     VIRDGYHVELDSLRTLRRDGSTWLAQLEARERARTGIDSLKIRHNHVFGYYLEVTKAN
                     AARVPPDYQRRQTLVNAERYVIPELLDLEAKVMSADEKSRALEIRLVEELREAVVEHA
                     PRIQAHARRLAALDAAAGLAESASRNGWTRPTLVEEPVLRIREGRHPVVERGLPAQRF
                     VPNDTDMDGESARLMLLTGPNMAGKSTYMRQVALIVIMAQMGSYVPADEAVIGLVDRL
                     FARVGAHDDIAGGRSTFMVEMSETAAILARATPRSLILLDEIGRGTSTFDGISIAWAV
                     AEDVHTRIGARTLFATHYHELTTLAESLPGLANYKAAVREWNDEIVFMRKIVAGGADR
                     SYGIQVARLAGLPASVIRRAREILNELERPGSRPAPPLADGQPDLFAAPLHPAVVEIA
                     ALDIVNLTPVAALNLLDRLKREIGGSGHPPQRG"
BASE COUNT          722 a         1532 c         1707 g          832 t
ORIGIN      
        1 tgattatcgt ggcgctcggc ctggccatgg agcggctcgt gttcgcgccg ctggagcatc
       61 gcgtccgtcg gcgctgggga ctggaaaggg cgtaaggccc gacacggaga aggggcggtg
      121 gggagagcgg gagataacga cgagaccccc tcaccccgtc atcccgtttc cccgtccctt
      181 cgttgttccg acagtggacc gcactgggag cattttcctc accaatccga tatcggcgtg
      241 cgcggagtgg gcgcgtcacc ggccgaagca ttcgagcagg cggccctcgc gcttattgcg
      301 gttatgagcg accccgccac ggtcgagcca cgggaagccg tggctgtgac gtgcgacgcg
      361 cccgacctgg aattattgtt cgtggaatgg ctggatcggt tgatttacga ggtcgcgacg
      421 cgccacatgc tcttcgggcg gtttaccgtg cgcatcgatg gactccggtt aaccgggcag
      481 gcgtggggcg aagcgatcga ccccgcccga catccttgcg tggtcgaggt gaagggagcg
      541 accttgacca gcttgggcgt ggcgcagggc gccgacggcc ggtggaccgc gcaatgcgtg
      601 gtggacgtat gaggaagccg tggatctcgg acgattgatc agggtgtcgg acaccgagtg
      661 gcacatcgag cgccacggcg ccatgcgcgt gccgggcgtg atctacgcca ccgaatcgtt
      721 gatccgggag atggacgaca aggtctacga gcaggtgacg aacgtggcga ccttgccggg
      781 catcgaggtc gggtcgttcg cgatgccgga cgcgcactgg ggctacgggt ttccgatcgg
      841 gggcgtggcc gcgttcaacc cgaccgaggg cggcgtgatc tccgccgggg gcgtggggtt
      901 cgacatttcg tgcggcgtgc gcgccctgtt gaccggcctc cggcgcgagc aggtcgagcc
      961 ggtcaagatg gcattggccg atgccttgtt tcgagccgtg ccggcgggcg tggggagtac
     1021 gggactcatc cgtctctcgg ccgcggaaat ggacgccatg ctcaggggcg gcgcgcaatg
     1081 ggccgtcgcg caagggtacg gctctcaggc cgatctggac cggatcgaaa gcggcgggcg
     1141 cactccggac gcggatcctg gggaggtgtc ggagtacgcc aaacgtcggc agcgcgatga
     1201 aatgggcacg ctgggatcgg gcaaccatta tctggaggtc cagcacgtgg cccgggtgca
     1261 cgatgcggcg ctcgccgagg cgttcggcct tcacgaaggc gacgtgatcg tgagcatcca
     1321 ctgcgggtct cgcggattgg gccatcagat cggcaccgaa ttcctcaagc gaatggcgac
     1381 cgaagcgccg caacacggaa tccatctgcc ggaccgcgaa ctcgcgtgcg ccccgatcga
     1441 atccgcgctg gggcgcgcat acctcggggc gatgcgcgcc gccatgaact gcgccatggc
     1501 caaccgccag atcattacgc acctggtgcg gcaggcggtg gccggcgtgt tgccggacgc
     1561 caggctgccg ctgctctatg acgtgtctca taacacgtgc aaagtggagg agcatcccgt
     1621 cgacgggcgc ctcacacggc tgtatatcca ccgcaaaggc gccacgcgcg cgttcggccc
     1681 cgggcatccc gatattccct ccgcgctccg cgcggtcggg caacccgtgt tgatcggcgg
     1741 gaccatgggc accgcatctt atatcttatg cggcacgcgc gcgggcatgt cgcgctcatt
     1801 cggttcggcc tgccacggag ccggacggag catgagccgg catcaggcca cgcggcagtg
     1861 gcggggcagg gacgtggtgg atgatctggc ccgtcgcggc atcatcgtac gcagcccgtc
     1921 cctccgcggc gtggcggaag aagcgcccgg agcgtacaag gacgtgagcg aagtggtggg
     1981 cgccgcggac cgcgcgggcc ttgcgcgaac cgtggcgcgg ttggagcctc tggtctgcat
     2041 caaggggtga cggtcggcgc cggccgttga ccgttcgctg gccgactggt atataccatg
     2101 ctggaacgtc ccagggaggg gtcctcatgg cccgcggtcg tcggttctgg ttgatgaagt
     2161 ccgagcccga agtgttttcg atcgaggatc tgcggcgggc cgacggtcag accacgtgct
     2221 gggacggggt tcggaactac caggcccgca acttccttcg cgacggaatc aagaccggtg
     2281 acggcgtgct gtactatcac agcaacgcgc agccgcccgg gatcgcgggc gaggccgtgg
     2341 tggttcgcga cgggtatccc gatttcaccg cgttcgaccc ggatgacccg cattacgata
     2401 cgaagagcga tcccaatcgt cccacctggt acatggtcga tgtgcggttc gtgcgcgcgt
     2461 gtaaggcgtt gatcgcgctc gatcggctca aggcgatctc gacgctccgt tccatgctcg
     2521 tgcttcggcg cgggatgcgc ctgtccgtcc agcccgtgac caccaaggaa tgggacgcca
     2581 tcatggcgct cccggagtgg gggtgacatg acgggccggc cgtcgtcgtg gttcgccaaa
     2641 tggggcttcc tcacggccct gatcggagct gcggcctgcg cgttcgcggg atggggaacc
     2701 cgcgccgaac tgtggtcgtt cggagcgggt ctgcgcgtgc tccgattggc ggcagtcggc
     2761 gccctgttcg ggagcgcgtt gtcgttgatc gggctgatcg ccacgcgcgg gggaaagaag
     2821 cgcggacgtt tttccgcgat cctgggggta gtgctcggcc ttgcggcggc cggcctccct
     2881 gcggcgcagg acatgaaggc gcggaccctc ccggcgatcc acgacatcac cacggacctg
     2941 gagaatcccc cggcgttctc ggccgtggtg ccgttgcgtc ggggcgctcc caacggggtg
     3001 gaatacgagg gggcgtcggt ggccgatcaa cagcgccggg cctatcccga tatccagccc
     3061 ctgtcgctcg tcgtccccgc caatcaggcg ttcgaccgcg ctttggaggc ggcgcgcgag
     3121 atggggtgga ccatcgtgga cgccaacccc gcgcacgggc ggatcgaggc cacggccacc
     3181 acgttctggt tcgggttcaa ggacgacgtg gtggtgcgcg tctccgcgac cgactccggc
     3241 agccggatcg acgtgcggtc gctttcgcgc gtgggccgca gcgacatcgg tgccaacgcg
     3301 cggcgcattc ggtgttatct cgacaagttg tccgcggtcg gttaaccgcg ttgcggtgga
     3361 tgtcccgatc cgccgatctc gcgcttcaga cgatccagca ggttcaaggc cgcgaccggg
     3421 gtgagattca cgatgtcgag ggcggcgatt tccaccacgg cggggtgcag gggcgcggcg
     3481 aagaggtccg gctgcccgtc ggcaagcggc ggcgcggggc gcgatccggg acgttccaat
     3541 tcgttcaaga tttcccgcgc gcgacggatg acgcttgccg gcaggccggc gaggcgcgcc
     3601 acctggatgc cgtagcttcg gtccgcgccg cccgccacga tttttcgcat gaacacgatc
     3661 tcgtcgttcc attcccggac cgcggcctta tagttggcga gcccgggcag cgactccgcg
     3721 agcgtggtca gttcgtggta gtgggtggcg aagagcgtgc gcgcgccgat acgcgtgtgc
     3781 acgtcctccg ccaccgccca cgcgatgctg atgccgtcaa acgtgctggt cccccttccg
     3841 atctcatcga ggaggatcag gctgcgcggg gtggcgcgcg ccaagatcgc cgcggtctcg
     3901 ctcatctcca ccatgaacgt gctgcgtccg cccgcgatgt cgtcgtgagc gcccacgcgc
     3961 gcgaacagcc ggtcaaccag cccgatcacg gcctcgtccg ccgggacgta cgagcccatt
     4021 tgcgccatga tgacgatgag cgccacctgt cgcatgtagg tggactttcc cgccatgttg
     4081 gggccggtga gcagcatgag ccgcgcggac tctccgtcca tgtcggtatc gttcggcaca
     4141 aaacgttgcg cgggcaggcc gcgttcgacc accgggtgcc ggccttcgcg aatgcggagc
     4201 acgggttctt ccacgagcgt cggccgagtc cacccgtttc gcgacgccga ttcggccagt
     4261 ccagcggccg cgtcaagcgc ggccagccgg cgcgcgtggg cttgaatccg tggggcgtgt
     4321 tctaccacgg cttcgcgtaa ttcttcgacc aggcggattt ctagcgcgcg gcttttctca
     4381 tccgcgctca tcaccttggc ctccaggtcc agcagttcgg ggatgacgta gcgctccgcg
     4441 ttcaccaggg tctgtcgccg ctggtagtca ggcggaacgc gcgcggcgtt cgccttggtc
     4501 acctcgaggt agtagccgaa cacgtggttg tggcggatct tgagcgagtc gatgcccgta
     4561 cgcgcgcgct cgcgcgcttc gagttgcgcg agccaggttg agccgtcccg gcgcagggtc
     4621 cgcagggagt cgagttccac gtggtagccg tctcggatca cacccccgtc gcgcgcgctc
     4681 ggaggcggcg tgggcacgag cgccgtggtc agtcgatcga tcaattgtcc gaagtcttcc
     4741 cacccctcgc ccagcgcccg cacgcgcggc tcggcgccgc cggccaggat ctt
//
LOCUS       DBNGTT010000340         3643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_463371, whole genome shotgun sequence.
ACCESSION   DBNGTT010000340 DBNGTT010000000
VERSION     DBNGTT010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_463371"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="AC16BC_07640"
     CDS             complement(<1..1146)
                     /locus_tag="AC16BC_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241531.1"
                     /translation="MNILLALVGLLSIGGVGWLWTSGFAGLDRPQRVGFAYGVGAVWI
                     GVMMGVIPLVGLPLVPAVGWSVVGVPPVGWWVWRRLRGGVAAKDADRPIAAGRSGHAA
                     AIRDAWWWGAAGFVGLLAAIVVARTVVKPIQAWDAWSTYATKAKAIYLSATVPRSMFE
                     TVTAPNYPLGVPLQEVWMSWFVGAWDDVAVKLLFPGYLVALLCLVYGELRVRWAARVA
                     MGGTLFVGCLPFLLQHGQDGYTDLPLAYFVLGGAGALTRFLRHREGHALALGAIFAAG
                     MVWSREDGVLVVVINGALLLYDGAWSFGIRSARTWRALLGYVAVPAAVWGGWTLVKAL
                     WAIPANLSLSIAGMAAHLDRLGGAVDALLTALFLEGNWLILWALVFLA"
     gene            complement(1143..2300)
                     /locus_tag="AC16BC_07645"
     CDS             complement(1143..2300)
                     /locus_tag="AC16BC_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241532.1"
                     /translation="MERAPVPRPGRTWGVRVLAVGATVWLVWAVAALGSHRLPPALSP
                     LNPVWDFSDAATPAWSSPNGRAVRGPLGLLLSPTVEAPMLSSGPLGHDPAAFRRVHLR
                     LSVRERTTGRFGLVLRGREGTRRVVAPFEVRGGATFEDVALSLDIPDQPGFRVEEAVL
                     VPSTISQPAVVAWISAEPGDGSLATWAKELWSPSPGESAALAGFAMHTLPPPALDGRS
                     VWLFLIPIVVIAGSVARLVGDAPGPCAVIRRFAWGTVGTVWVLGAALATYHQTVALAT
                     DIDQFGGRTGDDVYALIDGAPLRDDMRAVAALIPAGDAVNFMTDARLDPTVSALWKGR
                     AGYYLAPIGVHPSGAVSLFYFGGPHQPCSAVAGDAAVLSDSERYCAWRTAR"
     gene            complement(2303..>3643)
                     /locus_tag="AC16BC_07650"
     CDS             complement(2303..>3643)
                     /locus_tag="AC16BC_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3241533.1"
                     /translation="DGTFEDVTAASRIRTAHYGMGCAAADYDNDGREDIVLTGHGGIS
                     LFHNEGRGRFEDVTESAGVRDAGWSTCAVWVDADGDGRLDLFVCHYVAWSPETDQVCR
                     LDARVKVFCGPDPYPPAAPRLWRNLGGGRFEDVTERAGLSKPGKALGAALLDADNDGR
                     VDLFLANDQMPNFLYRNRGDGTFEDWNTRLAPWPGRFGMARAGMGVDVDDAAGRIAVG
                     NFIGEGIALYQRDADGRFHDRTRETGLFAPSLPFLTFGLAFADMNLDGRLDVVAANGH
                     VDAELARALDGGASQQERPLLFLGTDDGAYIEAGAALGLTEPFVGRGLAVADLDGDGD
                     LDVVLTENAGRARVYRNDVPKGRWLRINLIGTSGNRDGVGAVVTVTAAGRTQTRMLRT
                     GSSYLSQSERAVVFGLAGSDRVDDVTVRWPGGAVSRLTNVAANQRLTIAEQAGP"
BASE COUNT          682 a         1402 c         1100 g          459 t
ORIGIN      
        1 cgccagaaac accagcgccc aaagaatcaa ccagttccct tccagaaaca gcgcggtcag
       61 caaggcatca acggctccgc cgagtcgatc caggtgcgcg gccatgccgg cgatcgagag
      121 cgacaaattc gcgggaatcg cccagagcgc tttcaccagc gtccacccgc cccacaccgc
      181 cgcgggcacc gccacatagc caagcaacgc gcgccaggtc cgcgccgatc gaatcccgaa
      241 gctccaggct ccatcataca gcagcagcgc cccgttgatc actaccacca ggacgccgtc
      301 ctcccggctc cacaccatgc cggctgcaaa aatcgccccg agcgccaggg catggccttc
      361 tcgatgacgg agaaagcgcg tcaacgcgcc ggcgccgccc aggacaaagt aggccaaggg
      421 aagatccgtg tatccgtcct ggccgtgctg cagcagaaac ggaagacagc cgacgaacag
      481 cgtgcctccc atcgcgaccc tggccgccca tcgtacgcgc aactccccgt acaccaggca
      541 cagcagcgcc acgagatacc cgggaaacag caacttgacg gcgacatcgt cccaggcgcc
      601 cacgaaccac gacatccaga cctcttgcag cggcactccc aacggataat tcggagccgt
      661 gacggtctcg aacatcgacc gggggaccgt ggcggagagg tagatggcct ttgccttggt
      721 ggcgtacgtc gaccacgcgt cccacgcttg aatgggtttg accacggtgc gagcgaccac
      781 gatcgctgcc agcagcccca cgaagccggc cgcgccccac caccacgcat cgcgaatggc
      841 cgccgcgtgg ccggatcgac ccgccgcaat cggtcgatcg gcatctttcg cggcgacacc
      901 tccacggagg cggcgccaca cccaccaccc gaccggaggc acgcctacca cactccagcc
      961 aaccgccggc accagcggca gcccgaccaa cgggatgacg cccatcatca cgccgatcca
     1021 gaccgccccg acgccgtacg caaaccccac ccgttgcggc cggtccaatc cggcgaaacc
     1081 cgacgtccac agccatccaa ccccgccgat gctcaacagt ccgacaaggg ccaacaaaat
     1141 gttcatcgcg cggtccgcca cgcacaatag cgttcgctgt cggacaagac cgcagcgtca
     1201 cccgccacgg ccgagcacgg ctggtgaggc cctccaaaat aaaagaggct caccgcgccg
     1261 gacggatgca ccccgatcgg ggcgaggtaa taacccgcgc ggcctttcca gagcgccgag
     1321 accgttggat cgagccgagc gtccgtcatg aagttgaccg cgtcgccggc ggggatgagc
     1381 gctgcgaccg cacgcatgtc atctcgcaac ggcgccccgt cgatcagcgc gtagacatca
     1441 tcccctgttc gccctccgaa ctgatcgata tccgtagcca gagccacggt ttgatgatac
     1501 gtggcgagcg cggcgccaag cacccacacc gtccccaccg ttccccacgc gaagcgtcga
     1561 atcaccgcgc acggcccggg agcatccccg accagccggg ccacgctgcc cgcgatcacg
     1621 acgatcggga tcaagaacaa ccagacggat cgcccatcga gcgctggggg agggagggtg
     1681 tgcatcgcga aacccgccag cgccgcggat tcacccggcg acggagacca caactccttc
     1741 gcccacgtcg ccagacttcc atcgcccggc tccgcgctga tccacgccac caccgcgggc
     1801 tgcgaaatgg tcgagggaac gaggacggcc tcttcgaccc ggaaacccgg ctgatcggga
     1861 atatccagcg agagcgccac gtcctcgaac gtcgcgccgc ctcgcacctc aaacggggcc
     1921 accacccggc gggttccttc gcggccgcgc agcacaaggc cgaatcggcc cgtggtgcgc
     1981 tcccgcacgc tcagccgcag atgcacgcgc cggaaggccg cgggatcgtg acccagcggg
     2041 ccgctcgaca gcatgggagc ctcgacggtc ggcgagagca gaaggcccag cggcccgcga
     2101 accgctcgac cgttcggaga actccaagcc ggggtggcgg cgtcggagaa atcccagacg
     2161 ggattcaagg gagacaacgc cgggggcagc cggtgcgacc ccaacgccgc caccgcccac
     2221 accagccaca ccgtggcgcc caccgcgagc acccggacgc cccacgtacg tccgggacgc
     2281 gggacgggcg cccgctccat ggtcacgggc ccgcctgttc ggcgatcgtg aggcgttgat
     2341 tcgcggccac gttcgtcagg cggctcacgg ccccgccggg ccaccgcacc gtcacgtcgt
     2401 ccacccggtc ggacccggcc aggccgaaca ccaccgcgcg ttcgctctgc gacagatagc
     2461 tggatccggt cctgagcatc cgcgtctggg ttcggcccgc cgcggtcacg gtcaccaccg
     2521 cgcccacccc gtcgcgattg cccgacgttc cgatcaggtt gatccggagc caccgtccct
     2581 tggggacgtc attgcgatag acgcgggcgc gtcctgcgtt ctcggtcaac accacgtcca
     2641 agtctccgtc cccgtccagg tcggccacgg ccaggccgcg cccgacaaac ggttcggtca
     2701 gccccagcgc ggcgccggcc tcgatgtagg ccccgtcgtc ggttcccaga aacagcagcg
     2761 ggcgctcctg ctgggacgcg ccgccgtcga gcgcccgcgc gagttccgcg tccacgtgcc
     2821 cgttggccgc caccacgtcc aaccgaccgt cgagattcat gtccgcgaac gcgagcccga
     2881 acgtgaggaa cggaaggctc ggcgcgaaca gtccggtttc gcgcgtgcgg tcgtggaacc
     2941 gcccgtccgc gtcgcgttga tacagcgcga tcccctcgcc gatgaagttc ccgaccgcga
     3001 tccgccccgc ggcgtcgtcg acgtcgacgc ccatgcccgc gcgcgccatc ccgaaccggc
     3061 ccggccaggg agcgagccgc gtgttccagt cctcgaacgt gccgtcgccg cggttgcgat
     3121 agagaaagtt cggcatctga tcgttggcca ggaacaggtc cacgcgaccg tcgttgtccg
     3181 cgtccagcag cgccgcgccg agcgctttgc ccggcttgga caagccggcc cgctcggtca
     3241 cgtcctcgaa tcggccgccc ccgaggttgc gccacagccg cggcgccgcg ggcggatagg
     3301 gatcagggcc gcagaacact ttgacgcgcg cgtcgagccg gcagacttga tcggtctcgg
     3361 gcgaccacgc cacgtaatgg cacacgaaca ggtccaaccg cccatcccca tccgcgtcaa
     3421 cccagaccgc gcacgtgctc cagcccgcgt cgcgcacgcc ggccgattcg gtcacgtcct
     3481 caaatcggcc gcggccctcg ttgtgaaaca gcgagatgcc gccgtgcccg gtcaggacga
     3541 tatcctcccg accgtcgttg tcataatcgg ccgccgcgca gcccatgccg tagtgggccg
     3601 tgcgaatccg ggacgcggcg gtcacgtcct cgaacgtccc gtc
//
LOCUS       DBNGTT010000341         2911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_463916, whole genome shotgun sequence.
ACCESSION   DBNGTT010000341 DBNGTT010000000
VERSION     DBNGTT010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_463916"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="AC16BC_07655"
     CDS             complement(<1..316)
                     /locus_tag="AC16BC_07655"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0106029 - tRNA pseudouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HIR3241534.1"
                     /translation="MSVLRLIIEYDGTDYHGWQRQASAATIQGTLEDAIRRISGKPAA
                     VVGAGRTDAGVHAAAQVAHVETAAALDPQTWRRALNAVLPADVKIIAVDRAPRSFHAR
                     FSA"
     gene            complement(334..1401)
                     /locus_tag="AC16BC_07660"
     CDS             complement(334..1401)
                     /locus_tag="AC16BC_07660"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR3241535.1"
                     /translation="MRRCLASLVWLSAWTILPDASAAPNTAVLMQQATACDQRLHASD
                     KLKRLRSSWQRCIERYADVAASAPQDTTGAFALKRQAELAEELARRSGRRDDARQAQR
                     LRERATRSGEQDNRQPAAPDAQKPVRVVIDPGHGGKDPGTMSAAGLQEKDVVLDVGVR
                     LQKLLERSDHIAPLLTRRTDQFISLEGRTEFARTRDADLFISIHANSSPRRDVKGLEV
                     YVLGESSDADADATASRENDVPGQETVDVGALVQDMLGDLSNSQRDEHSLELAHTLRQ
                     AVRQRVGPKYEIVDLGIKRAPFFVLLNSGVPSILSELAFMSNPDDANRLAQPTFRQRI
                     AEALAAGILQYTASPVIAQSN"
     gene            1578..>2911
                     /locus_tag="AC16BC_07665"
     CDS             1578..>2911
                     /locus_tag="AC16BC_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR3241536.1"
                     /translation="MRRRSATAVGLLGLFTALHLATSLPLDAQVVGQSTSPQERAALA
                     RAKAVLADGYTAEAARLLDRFLQTYPTSESRAEAATLLGHIRLQEKAYPQVIALLTEI
                     VESGADHEFVDRARLDLGAAYLGAGKKTQAGTVLGVLAASDAAPGLRRQAYDLLSGLA
                     LETRDVLRAVTWLMEARALPGDEGDLSALDTRLREVITGADDPRDVESVAEAFAGRFP
                     ADVALVRAAQLYGAGGDPFDQDRVLQTLMTTFPQHEAVADARRTVEANRARVRAAKFA
                     IALPLPYEGALQPYATSILRGAQLAIDTERAGGQELSVALAARDYGGDPSRLGTVLDD
                     ICREARCVGIVGPVLTREGAAVASRAASWRIPAVGPTPIGGAAPNRYVFRTGLTAQEE
                     GAAIARYAAEHLGMKRFTVLAPQDRYSQEVATAFGNEVGRLGGRLVYSGAYQT"
BASE COUNT          440 a          898 c         1047 g          526 t
ORIGIN      
        1 cggcggagaa gcgggcgtgg aagctgcgcg gagcgcggtc gaccgcgatg attttcacgt
       61 ccgccggcaa cacggcgttc agggcgcgcc gccaggtctg gggatcgagc gcggcggccg
      121 tttcgacgtg cgcgacctgg gccgcggcgt ggaccccggc gtccgtgcgc cccgcgccga
      181 ccacggcggc gggtttcccg ctgatgcggc gaatcgcgtc ttcgagagtt ccctgaatcg
      241 tcgccgcgga ggcctggcgt tgccagccgt ggtagtcggt cccgtcgtat tcgatgatca
      301 gtcggaggac ggacatcaag gtcgtccggg acgtcagttc gattgagcga tcaccgggga
      361 agcggtgtac tggaggatgc cggccgcgag ggcttcggca atgcgctgcc ggaaggtcgg
      421 ttgcgcgagt cggttcgcgt cgtcgggatt gctcatgaac gccaactcgg acagaatgct
      481 gggcactccg gagttcagga gcacgaagaa cggcgcccgt ttgatcccga gatcgacgat
      541 ctcgtatttt gggccgacgc gttgccgcac ggcttgccgg agcgtgtggg ccaattcgag
      601 ggagtgctcg tcgcgttgac tgttggacag atctccgagc atatcttgga cgagggctcc
      661 gacatcgacg gtttcctggc ccggcacgtc gttctcgcgg gaggcggtcg cgtcggcgtc
      721 ggcgtcgctg gactctccga ggacatagac ctcaaggcct ttgacgtccc gtcgggggct
      781 ggagttcgcg tggatcgaaa taaacagatc ggcgtcgcgt gttcgggcga actcggtgcg
      841 gccttcgagc gagatgaact gatccgtgcg tcgggtcaac aggggggcga tgtggtcgct
      901 tcgttccagc aatttctgga ggcgtacgcc gacatccagc accacgtctt tttcctgaag
      961 gccggcggcg ctcatggtgc ccggatcttt tcctccgtgt cccgggtcaa tgaccacgcg
     1021 gacgggcttc tgggcatccg gcgcggccgg ctgtcggttg tcctgttcgc ccgaccgggt
     1081 ggcgcgttcc cggagtcgtt gagcttggcg cgcgtcgtcg cggcgaccgg atcgccgcgc
     1141 caattcctcc gccaattccg cttgccgttt gagcgcgaac gctccggtgg tgtcctgcgg
     1201 ggcggaggcc gcgacgtccg cgtaccgctc gatgcagcgt tgccagctgg accggaggcg
     1261 tttgagtttg tcggatgcgt ggagacgttg gtcgcaggcg gtggcctgtt gcatcaagac
     1321 ggcggtgttg ggagcggccg atgcgtcggg gaggatcgtc caggcgctga gccacaccag
     1381 cgatgccagg catcgtcgca caaggagtcg gcgcgacatt tggcccgaac agtaacgtag
     1441 ggcttgcccg atgtcaattc ccgatcagcc ggcgccttga ttttaccgag aggttctcgt
     1501 ataaacgtca ccacgctccg gcgcggtatg gctcgcgcct caagtggaga ggattgaaga
     1561 cccctttcgc gacgggcttg cgccgtcggt ccgcaacggc cgtcggcctg ttagggctgt
     1621 tcacagccct gcatctggcg accagccttc cgctcgacgc ccaggtggtc ggccagtcga
     1681 caagcccgca agaacgcgcc gccttggcgc gcgccaaggc cgtgttggcc gacggctaca
     1741 ccgccgaggc cgcccggcta ctcgaccgat ttctccagac ctatccgacc agcgagtcgc
     1801 gggccgaagc cgcgacattg ttgggacaca tccggcttca agaaaaggcg tatccgcaag
     1861 tcatcgccct gttgaccgag atcgtcgaat cgggggccga tcacgaattc gtcgatcgcg
     1921 cgcgtctgga cctcggggcc gcgtatctgg gcgccggaaa gaagacgcaa gcggggactg
     1981 tgttgggcgt gttggccgcg tcggacgcgg caccggggct ccgacgacag gcgtatgacc
     2041 tgttgagcgg tctcgcgctg gagacccgcg atgtcctccg cgccgtgacc tggttgatgg
     2101 aagcgcgtgc gttgcccggc gacgagggtg atctgtcggc gctcgatacg cgtcttcgcg
     2161 aggtcatcac cggcgccgac gaccctcgcg atgtggagtc ggtcgcggag gcttttgccg
     2221 gccggttccc cgccgacgtc gcgctcgtgc gtgcggccca actctacgga gccggcgggg
     2281 acccgttcga ccaggaccgg gtgctgcaga cgttgatgac cacgttcccg caacacgagg
     2341 cggttgccga cgcgcgcagg accgtcgagg ccaatcgcgc ccgagtccgg gcggccaagt
     2401 ttgccatcgc gcttccgctg ccgtatgaag gggcgctgca accctacgcg accagtattc
     2461 tgcgcggcgc gcagttggcg atcgacaccg agcgggccgg ggggcaggag ctttcggtgg
     2521 cgttggcggc cagggattac gggggagatc cgtctcgatt gggcacggtg ttggacgaca
     2581 tctgccgcga agcccgttgc gtgggcatcg tggggccggt gcttacgcgt gagggggccg
     2641 cggtggcctc acgggccgca tcgtggagaa ttcccgcggt cgggccgacc ccgatcggcg
     2701 gagccgctcc aaaccggtat gtcttccgga ccgggctcac ggcacaggag gaaggggcgg
     2761 cgatcgcccg gtacgccgcc gagcatctcg ggatgaaacg attcaccgtg ctcgcgccgc
     2821 aagaccggta ctcgcaggag gtggcgacgg ctttcggcaa cgaagtcggt cgtctcggcg
     2881 gacgactggt gtattctgga gcctatcaaa c
//
LOCUS       DBNGTT010000342         2798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2126116, whole genome shotgun sequence.
ACCESSION   DBNGTT010000342 DBNGTT010000000
VERSION     DBNGTT010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2126116"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC16BC_07670"
     CDS             complement(<1..257)
                     /locus_tag="AC16BC_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016870292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3241537.1"
                     /translation="MTEHKHDTDHPVREIPDTLPVLPLRDAVVFPFAVVPLSVGQQRS
                     VQLVDDAMKGNRMIAVVYQLSPDAVPAGPNDVHRVGTAAVV"
     gene            complement(290..667)
                     /locus_tag="AC16BC_07675"
     CDS             complement(290..667)
                     /locus_tag="AC16BC_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3241538.1"
                     /translation="MRYRRISYRYTEVVTQHTVRLLGDPWGLLSRTTVARPEWKPPAD
                     LYEADDTYVVKVELAGVAEDDLDVSVYTDAIVVEGVRPIGVTGEVRYHTAEIRYGPFR
                     LEVANPTAINRERLSARYERGFL"
     gene            977..1576
                     /locus_tag="AC16BC_07680"
     CDS             977..1576
                     /locus_tag="AC16BC_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3241539.1"
                     /translation="MATQPNQTTIQPGDALLVVDVQRDFLPGGSLAVPSGDAVIPALN
                     RYLALWRARGLPVFATRDWHPAKHASFREQGGPWPPHCVAGTPGAAFDPALALPSNAV
                     VISKGDAADRDAYSGFQGTTLHERLRAVGVRRVFVGGLATDYCVLATVRDAQALGYAV
                     GLLTDAIKAVDVHPGDGQRAEDEMIRLGAEPVRYEQIAP"
     gene            1573..>2798
                     /locus_tag="AC16BC_07685"
     CDS             1573..>2798
                     /locus_tag="AC16BC_07685"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR3241540.1"
                     /translation="MTPEPSVLLTDLYQLSMLDVYRRQGMEQIAVFEFFVRKLPASRR
                     FLLAAGLEQLLAYLETLRFTSEDLAWVEASGRFGPGFADYLRDFRFTGDVHAMPEGTV
                     CFPDEPLVRVTAPLPQAQLVETRLINIIQLQTLLASKAARFALVAPEALLVDFGLRRA
                     HGAEAGLLGARAVYLAGFSGTATVSANPAFGIPVYGTMAHSFIQAHDDEAEAFAAFAR
                     HQSQNVVLLLDTYDTEAAAEKVVALAPRLAAEGITVKGVRLDSGDLGEHARKVRRILD
                     AGGVTNITIFASGNLDEYAVRDLVRGGAPINGFGVGSRLMTSSDAPYLDCAYKLQEYA
                     RRARRKRSEGKATWPGRKQVYRRYGPDGRMLDDVLTVEGDVQEGEPLIVPVMRAGKRL
                     GPPRPLNEIREYARVQ"
BASE COUNT          427 a          921 c          986 g          464 t
ORIGIN      
        1 tggaccaccg cggcggtgcc cacgcgatgc acgtcgttgg ggccggcagg caccgcgtcc
       61 gggctcagtt ggtagaccac cgcgatcatg cggttgccct tcatcgcatc gtccacgagc
      121 tggaccgagc gctgctggcc gaccgacagc ggcaccacgg cgaacggaaa cacgaccgcg
      181 tcccgcagcg ggagcacggg caacgtgtcc gggatctccc ggaccgggtg gtcggtgtcg
      241 tgtttgtgct cagtcatgac cggccccccg tgtccttgcc gacggtcagc tagagaaagc
      301 cgcgttcgta ccgcgcggac agccgctcac ggttgatcgc ggtggggttc gccacttcga
      361 gccgaaacgg cccgtagcgg atctccgcgg tgtggtaacg cacctcgccg gtcaccccga
      421 tcggacggac gccctcgacc acgatggcgt cggtgtagac cgagacgtcg aggtcgtctt
      481 ccgccacgcc ggccagttcc accttgacca cgtaggtgtc gtcggcttcg tacagatcgg
      541 ccggcggttt ccactcgggc cgcgccacgg tcgtgcgcga caacaggccc cacggatcgc
      601 cgagcagccg caccgtatgc tgcgtgacca cctcggtata tcggtaactg attcgccggt
      661 accgcattca gtcttctcct tcgcgtgtct gacaggatgc cggaacgcgc cgcgtcttgc
      721 cattcctcaa ggggaggtgg ttccccgctc caagggagtg gcgcacgagc tggatagtac
      781 tccaaacgag tggacggagt caggcccatg catgaggccc gtatcccgcg cttggggaat
      841 ggcattcgtg gcccgcgacc gcgagaatgt cgcgtgatgg cgcgtgttgc gcgtggtggt
      901 ccgtgggcgt caccgcggtc ggcaaacggc tccggcctgg acatccgccg gcgcaatcag
      961 gcaggatgaa gaccgcgtgg ccactcaacc caaccagacg accattcaac cgggcgacgc
     1021 gctgctggtg gtggacgtgc agcgggactt cttgccgggc ggaagcctgg ccgtgccctc
     1081 gggcgacgcg gtgatcccgg cgctcaaccg ctatttggcc ctgtggcgcg cgcgcggatt
     1141 gccggtcttt gccacccgcg actggcaccc ggcgaagcac gcgtcgtttc gtgagcaggg
     1201 cggaccatgg cccccgcact gcgtggcggg gactccgggc gcggcgtttg atccggcgct
     1261 cgcccttcca tcaaacgcgg tcgtgatctc gaagggggac gcggcggatc gcgacgcgta
     1321 ttcgggattt cagggcacga ccctgcacga gcgcttgcgg gccgttgggg tgcgccgcgt
     1381 gttcgtgggc ggcctggcca cggactactg cgtgctcgcc accgtgcgcg acgcccaggc
     1441 gttgggctac gcggtcgggt tgctgacgga cgcgatcaag gccgtggacg ttcaccccgg
     1501 cgacgggcaa cgcgcggagg acgagatgat ccgcctcggc gccgagccgg tccgctacga
     1561 gcagatcgct ccatgacacc cgagccgagc gtcctgctca cggacctcta tcaactctcc
     1621 atgctggacg tgtatcgacg ccaaggcatg gagcagatcg cggtgttcga gttctttgtc
     1681 cgcaaattgc ccgcgtcccg ccgtttcctg ctggccgcgg ggctcgaaca gttgctcgcg
     1741 tatctcgaga ccctgcgctt cacatcggaa gacctggcgt gggtcgaggc cagcggacga
     1801 ttcggtccgg ggttcgcgga ctatttgcgg gactttcggt tcaccgggga cgtgcacgcc
     1861 atgcccgagg gcacggtgtg ttttcccgac gagccgttgg tgcgggtgac cgcgccgctc
     1921 ccgcaggccc aactggtcga gacccggctg atcaacatca tccagttgca gaccctgctg
     1981 gcgtccaaag ccgcgcgctt cgcgctggtg gcgccggagg cgctcttggt ggacttcggt
     2041 ttgcgacgcg cgcacggcgc cgaggccggg ctgctgggcg cgcgcgccgt gtacctggcg
     2101 ggattttcgg gcactgccac cgtgtccgcc aatcccgcgt tcgggatccc ggtctacggc
     2161 accatggcgc attcgttcat ccaggcgcat gacgacgagg ccgaggcgtt cgccgccttc
     2221 gcccgccatc aatcccagaa cgtggtgctg ctgctggaca cctacgacac cgaggccgcg
     2281 gccgaaaagg tggtggcgct cgcgccgcga ctcgcggccg agggcatcac tgtcaaaggc
     2341 gtgcggctcg acagcgggga tctgggcgag cacgcgcgca aagttcgccg gatcctggac
     2401 gccggggggg tgacgaacat caccatcttc gcgagcggga acctggacga gtacgcggtg
     2461 cgggatctgg tgcgcggggg cgcacccatc aacgggttcg gcgtgggctc gcggctgatg
     2521 acatcgtcgg acgcgccgta tctggattgc gcgtacaaac tgcaggagta cgcgcggcgg
     2581 gcgcggcgca agcgatccga aggcaaggcc acctggcccg gtcgcaagca ggtctaccgg
     2641 cgctacggcc cggacggccg gatgctggac gacgtgctca cggtggaggg cgacgtgcag
     2701 gagggcgagc cgctgatcgt gccggtgatg cgggccggca aacgcctggg accgccgcgg
     2761 ccgctcaacg agattcggga gtatgcgcga gtgcaatt
//
LOCUS       DBNGTT010000343         2746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2253352, whole genome shotgun sequence.
ACCESSION   DBNGTT010000343 DBNGTT010000000
VERSION     DBNGTT010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2253352"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="ntrB"
                     /locus_tag="AC16BC_07690"
     CDS             complement(<1..659)
                     /gene="ntrB"
                     /locus_tag="AC16BC_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HIR3241541.1"
                     /translation="MLPLVGVAVLVAIWAMVSQLAPFLPNPIQTVTKSWDLIRHPFFD
                     EGPNNKGLGWQVLYSLGRVGLGFGLAILVGIPAGLMLGLSSVTRKMWDPVVQVLKPVS
                     PLAWLPLGLATFQAATPAAVFTIFITALWPILINTSAGVQQVPVTYLQVGRILRLSRR
                     KMVTRILIPAALPYILSGLRVSLGVAWLVIVASEMLTGGMGIGFFVWDEWNNLSLEHI
                     IL"
     gene            complement(782..2068)
                     /locus_tag="AC16BC_07695"
     CDS             complement(782..2068)
                     /locus_tag="AC16BC_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3241542.1"
                     /translation="MSNASDQSHKTQDYSRRDVIKTAGVTALGVLLAGLPPLRGGLFA
                     GTGDAPEVAKLKIGFIPLTDCASVVMAYELGLYKKYGLDVTISKEASWAAVRDKLTIG
                     DIQASHILYGMPYASTLGIVGAEGQQKAMVIPMALNQNGQAITLGNHLKKQGVKTAQD
                     VKKLVDADKGKKTYTFAMTFPPGTHAMWMRYWLASGGVDPDNDIKLITIPPPQMVANM
                     RIGNMDGFCVGEPWNARAIFDGIGFSAISTQQMWVNHPEKVLGMTQEFAEKNPKTVKA
                     ILMAVIEASQYIDKMENRSKVAETIGKKEYVNAPFDVVLGRMQGKYVYGDGRTEQDPD
                     YMKFYRDGEVTFPWKSHGLWFLTQHRRWGFLKEPIDYKKVVDSVNRTDLYRDAAKALG
                     TPVPAEEYKKETLFDGIEFDPADPEGYVKKFAIKRV"
     gene            complement(2084..>2746)
                     /locus_tag="AC16BC_07700"
     CDS             complement(2084..>2746)
                     /locus_tag="AC16BC_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024765.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HIR3241543.1"
                     /translation="TAPAATGQRRTTLGLRIDGKAVNESVDFTGEAAYQSGAMTARGT
                     TPESDISAYAMAIKAGYTVPVDLKPRIGIEYDLASGDSNSADDTFETFENLFPTNHPF
                     YGIMDYVGWRNMQDLRLSLSAKPSATTGVSLDYHKFSLAEKTDNWYQASGAVFRTTPG
                     GNTESDLGQEIDLVGYVMVKEKVRIEAGYGRFMPGDYVDVNFPTANDASDWLYLQVGT
                     TF"
BASE COUNT          493 a          860 c          821 g          572 t
ORIGIN      
        1 agaatgatgt gctccaacga cagattgttc cactcgtccc agacaaaaaa cccgataccc
       61 ataccgccgg tgagcatctc ggacgccacg atcacgagcc aggccacgcc caggctcacc
      121 cgcaatccgg acaagatgta cgggagcgcg gcggggatca ggatgcgcgt gaccattttg
      181 cggcgcgaga gtcggaggat gcgaccgacc tgaagatacg tgaccggcac ctgctggact
      241 ccggccgagg tgttgatcag gatcggccat aacgcggtga tgaagatggt gaagaccgcg
      301 gccggcgtgg cggcctggaa cgtggccagg cccaacggca accatgccaa gggggagacg
      361 ggtttgagga cctggaccac cgggtcccac atttttctcg tgaccgaaga cagccccaac
      421 atcaggccgg ccgggatgcc gaccaggatg gcgaggccga atccgagccc gacgcgtccc
      481 aggctgtaga gcacctgcca ccccagtccc ttgttgttgg ggccttcgtc aaagaacggg
      541 tgacggatca gatcccaact cttggtcacg gtctggatgg gattgggcag gaacggtgcg
      601 agctggctca ccatggccca gatggccacg agcacggcca cgccgaccag aggcaacatg
      661 atgtcgcgga tcggtatccg tcgaaatccc cacgaggtaa atcccccccc gccccccttt
      721 ttcaaagggg ggactttctc actgaccgaa ggtattcgct gtgcctcgac tcgctccata
      781 ctcacaccct cttgatcgca aacttcttga cgtagccttc cggatccgcg gggtcgaact
      841 cgatcccgtc gaacagcgtc tccttcttgt actcttcggc tggcaccggg gtgcccaacg
      901 ccttggcggc atcgcgatac aggtccgtgc gattgaccga gtccacgact ttcttgtagt
      961 cgatgggctc cttgagaaat ccccaccgtc ggtgctgcgt caggaaccac aggccgtggg
     1021 acttccacgg gaacgtgact tccccgtccc ggtagaactt catgtagtcg gggtcttgct
     1081 cggtgcgtcc gtctccatac acgtatttgc cctgcatgcg tcccaacacc acgtcgaacg
     1141 gggcgttgac gtattccttc ttcccgattg tctccgcgac cttggatcga ttctccatct
     1201 tgtcgatgta ctgcgatgcc tcgatcaccg ccatcagaat agccttcacg gtcttggggt
     1261 tcttctccgc gaactcctgg gtcatcccta acaccttctc aggatgattg acccacattt
     1321 gctgcgtgga aatggcggaa aatccgatgc cgtcgaagat cgcgcgcgcg ttccagggct
     1381 cgcccacgca gaacccgtcc atgttgccga tccgcatgtt ggccaccatc tggggcggag
     1441 gaatcgtaat cagcttgatg tcgttgtccg ggtccacgcc gcccgaggcc agccagtacc
     1501 gcatccacat ggcgtgggtg ccgggcggga aggtcatggc gaacgtgtac gtcttcttgc
     1561 ccttgtccgc gtccaccagt ttcttgacgt cttgcgcggt tttcacgccc tgcttcttga
     1621 ggtggttgcc caaggtgatg gcttggccgt tctggttcaa cgccatcgga atgaccatcg
     1681 ccttttgctg gccttcggcg ccgacgatcc cgagcgtgga ggcgtagggc attccgtaga
     1741 ggatgtggga ggcctgaatg tctccgatcg tcagcttgtc gcgaaccgcg gcccacgacg
     1801 cctctttgga gatggtcacg tccaggccgt acttcttgta cagccccagt tcataggcca
     1861 tgaccaccga ggcgcagtcg gtcaggggaa tgaagccgat cttgagtttg gcgacctccg
     1921 gcgcgtcgcc ggtgccggcg aacagcccgc cccgcaatgg cggaagtccg gccagcaaca
     1981 cgccgagcgc ggtcacgccg gcggtcttaa taacgtcgcg gcgggagtaa tcttgggtct
     2041 tgtgtgattg atccgatgcg ttcgacatgt gtccctcggc tccctagaaa gttgttccga
     2101 cttgcagata cagccagtcc gacgcatcgt tcgcggtcgg gaagttcacg tccacgtagt
     2161 cacccggcat gaagcgtccg tacccggctt cgattcggac cttctctttg accatcacat
     2221 accccaccag gtcgatctcc tggcccaggt cggattccgt gttgcctccg ggtgtggtgc
     2281 gaaacaccgc gccggacgcc tgataccagt tgtcggtttt ctcggcgagg gagaacttgt
     2341 ggtagtccag gctcacgccg gtggttgcgc tcggtttggc gctcaatgac aaccgcaggt
     2401 cctgcatgtt ccgccatccc acgtagtcca tgatgccgta gaagggatgg ttggtgggaa
     2461 agaggttctc gaaggtctcg aacgtgtcgt cggcgctgtt gctgtctccc gacgccagat
     2521 cgtattcgat tccgatgcga ggcttcaggt cgaccggcac ggtgtagccg gccttgatcg
     2581 ccatggcgta tgccgaaata tcgctctcag gcgtggtgcc ccgcgccgtc attgcccccg
     2641 actggtaggc ggcctcaccg gtaaagtcga cggactcgtt gaccgccttc ccgtcaattc
     2701 gaagccccaa cgtggtccga cgctgcccgg tggcggccgg cgcggt
//
LOCUS       DBNGTT010000344         4207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2891680, whole genome shotgun sequence.
ACCESSION   DBNGTT010000344 DBNGTT010000000
VERSION     DBNGTT010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2891680"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="AC16BC_07705"
     CDS             <1..835
                     /locus_tag="AC16BC_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241544.1"
                     /translation="PWLTTIGLLQEHRMGFSAAGLAVASAALGRLLWDGAARRKTGRV
                     MRWTAAIAAITAVMGAIAVDRSRAAVWRDDRRLWGEVVGWSPDNMLARINLGAAYMEH
                     DEYDRAEAEYRAIIALTPAYPRAYYNLGLLALRRGRTDDAVAAFERAAALDPGNASPR
                     THLGILALRSGDAQRAEREFEAALGLDPTQRDALNNLSAIYLERREWSKALSLVDEAL
                     ARDPGFLEAAYNRGVALAGLGRRAEATVALTDVRRRLPPDAKFDQYRSGIDHLLGSVQ
                     P"
     gene            832..2781
                     /locus_tag="AC16BC_07710"
     CDS             832..2781
                     /locus_tag="AC16BC_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241545.1"
                     /translation="MRTPWAAVLIAGAIVAAHGGAVRGAFHYDDMPTVSDNLAIRTFQ
                     PLVYLTSPSAATAEPGSAGFRPVTVLTFAGNYALGRLVPAGYLAVNLVLHALIAWMVF
                     VTGRTLLRDDAWALFAALVYAVHPLNAESVNYVVARSSLLAALGSLVTVWAFFRRRAG
                     GGWWWTGVSVAAFVMALLSKESGVAVLIPLAAAVVMDPVAGAPRHRRKASEIVREAWQ
                     ALWPYGIAASLYLVAWWLVAGAHAEQHGRAAAYPVWAFAEIVGRSMLLWVWPWPLGLD
                     HPVAFASGFSAWTAGWLAAWLAVLIGAALVYRTRRPIVSWCLLWALAGLVPLAPLPWI
                     TVKGLMQENRMVFSAIALAWLTAVVARETLSAYHRAASARRWAVCDRLVRGGAMASAV
                     VVLIAAAAMDRARSAVWADDARLWREMVARSPESRTGYTNLGAAYMARNEYDRAEEAL
                     RRAVAIAPNDAFPYYALGMLAYRREQYDAARMLFVKSATLAPEYAKTYRMLGIIAIKQ
                     HRDQDGALFLRRALTLDPRDATAMANLGLAAQRAGDEAAARRLYRDALALDPAQRLAR
                     NNLGTLYLQGRQWGDALEQFSALLAAAPDDYDAALNRAVALHEMGRKDDARGAFEALL
                     ARLPPDPKFDMHRRAAELILRRTAP"
     gene            2778..>4207
                     /locus_tag="AC16BC_07715"
     CDS             2778..>4207
                     /locus_tag="AC16BC_07715"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR3241546.1"
                     /translation="MSPFAWLSLVFVLSGASGLIYQVVWGRLLVLVFGSTTIAVTTVL
                     AVFMGGLAIGSALGGRVVDRVRRPLLAYAVIEAAVAAYALAIPWLLHAVVPVYQAVWR
                     ELSTSPLSVMAARVGLVSAILAVPTVLMGATLPVISRVVAAGATSIAGRVSALYTLNT
                     AGAIAGAVGAGFLLLPAVGVTATIVVAAVGNAAAAITAWALVRRGFDSTSRSPRVDAA
                     GSESGEAGVRRRVAVTLAISGAAALVNEVVWTRALSLVLGSSVYAFSAMLTTFLSGLA
                     AGAACGTWLVRRTSPRFATLGAVQLATALASLVTLALIGRLPLALIAAVGSLRGLGAD
                     VIPAVQFALSFLVMFLPTFGFGVVFPVALSLAVRSPDAVGTSVGRLYAANTAGAIAGA
                     VVAGFVLVPLVGVPGSLVAAAATCAVLGLWLVVGDRSIARRLAWGGVLGSAVLGALVL
                     GAIPPWSGTLMSSGVYWNLPRYLALA"
BASE COUNT          474 a         1328 c         1624 g          781 t
ORIGIN      
        1 tccctggctg acgacgatcg gtttgcttca agaacaccgc atgggttttt cagccgccgg
       61 tctggccgtg gcgagcgccg cgttgggccg tctgctctgg gacggagcgg cgcgccgaaa
      121 gaccggccgg gtgatgcggt ggaccgcggc gatcgcggcg atcaccgcgg tcatgggggc
      181 gatcgccgtg gatcggtcgc gtgccgcggt ttggcgggac gatcgccgtc tgtggggtga
      241 ggtcgtgggt tggtcgcccg acaatatgtt ggcccggatc aacctgggcg ccgcctacat
      301 ggaacacgac gagtatgacc gcgccgaagc ggagtatcgc gcgatcattg cgctgacgcc
      361 ggcctatccc cgcgcgtact acaatctcgg gctgctggcg ttgcgtcgcg ggcgcacgga
      421 cgacgcggtc gcggcgtttg agcgcgcggc cgcgctcgat cccggcaacg cctcccccag
      481 gacgcacctg gggatcctgg cgcttcggtc gggcgacgcg caacgagccg aacgggagtt
      541 cgaggcggct ttgggcctcg atcccacgca acgcgacgcg ttgaacaatc tgtccgccat
      601 ttacctggag cggcgcgagt ggtccaaggc cttgagcctg gtggacgagg cgttggcgcg
      661 cgacccgggg tttttggagg cggcgtacaa tcggggggtg gcgttggccg ggctcgggcg
      721 acgcgcggag gcgaccgtgg ccctcacgga cgtccggcgg cgcttgccgc ccgacgcgaa
      781 gttcgaccag taccgatccg ggatcgacca tctgctggga agcgtgcagc cgtgagaacg
      841 ccgtgggcgg cggttctcat cgcaggggcg atcgtggccg cgcacggcgg cgccgtgcgc
      901 ggggcctttc attacgacga catgcccacg gtctcggaca atctcgcgat ccgaacattt
      961 caaccgttgg tgtacctgac ctctccgtcc gcggcgaccg ccgagccggg ctccgcgggg
     1021 tttcgtccag taacggtctt gacgttcgcc gggaactacg cgcttggtcg tctggtgccc
     1081 gccgggtact tggccgtcaa cctggtcctg cacgcactca ttgcctggat ggtgtttgtg
     1141 acgggccgga ccctgctgcg cgatgacgcg tgggcgttgt ttgccgcgct cgtgtacgcg
     1201 gtgcatccgt tgaacgcgga gagcgtcaac tacgtggtgg cccggtcctc gctgttggcg
     1261 gcgctcggca gcctcgtaac cgtgtgggcc ttcttccgcc gtcgagcggg cggcggctgg
     1321 tggtggaccg gtgtgagcgt ggcggcgttc gtcatggcgc tgctcagcaa ggagtcgggc
     1381 gtggccgtgc tcatcccgct ggcggcggcc gtggtcatgg accccgtggc cggcgcgccg
     1441 cgccatcgcc gcaaggcgtc ggagatcgtg cgggaggcct ggcaagcctt gtggccctat
     1501 gggatcgcgg ccagcctgta cctcgtggcg tggtggctcg tggccggcgc acatgccgag
     1561 caacacggcc gtgcggccgc ctaccccgtc tgggcctttg cggagatcgt gggccgttcg
     1621 atgctgctgt gggtgtggcc gtggcccctg ggattggatc accccgtggc gttcgcctcg
     1681 ggattcagcg cctggaccgc ggggtggctc gcggcgtggc tggcggtgtt gatcggcgcc
     1741 gcactcgtct atcggacccg gcggccgatc gtgtcgtggt gtctcctgtg ggcgctggcc
     1801 ggactcgttc cgctggcgcc gctcccgtgg atcaccgtca agggtctgat gcaggaaaat
     1861 cggatggtgt tttccgccat tgcgctcgcg tggcttacgg cggtcgtggc gcgcgagacg
     1921 ctgtctgcgt atcatcgcgc ggcgtcggct cgacgctggg ccgtctgcga ccgtctcgtg
     1981 cgcggcggcg cgatggcgtc cgccgtggtg gtcctgattg ccgcggccgc gatggatcgg
     2041 gcgcgatccg cggtgtgggc cgatgacgcg cggctgtggc gggagatggt ggcgcgttca
     2101 cccgagagcc ggaccgggta cacgaacctg ggcgccgctt acatggcgcg caacgagtac
     2161 gatcgggccg aggaagcgct caggcgggcc gtggcgatcg cgccgaacga cgcgttcccg
     2221 tattatgcgc tggggatgtt ggcgtaccgt cgcgaacaat acgatgccgc ccggatgctg
     2281 ttcgtgaaaa gcgcgaccct ggccccggag tacgcgaaaa cctaccggat gctggggatc
     2341 atcgcgatca aacaacaccg ggatcaggac ggcgcgctgt ttctccggcg cgcgctcacc
     2401 ctggatccgc gggacgccac ggctatggcc aatctggggc tcgcggcgca acgcgcgggc
     2461 gacgaggcgg cggcccgccg tttataccgt gacgcgctcg cgctcgatcc ggcgcagcgg
     2521 ctcgcccgga acaatctggg gaccttgtac ctccagggcc gccagtgggg cgacgcgttg
     2581 gagcagttct ccgcgttgct cgcggccgcg ccggacgact acgatgcggc attgaaccgc
     2641 gcggtggcgc tccatgaaat gggccgtaag gacgacgccc ggggcgcgtt cgaggcgttg
     2701 ttggcgcgcc ttccgcctga tccgaagttc gacatgcatc ggcgcgccgc cgaactcatc
     2761 ttacgccgga cggctccgtg agcccgtttg cgtggctgag tctggtgttc gtgctgtcgg
     2821 gcgcgtccgg actgatctat caggtggtct ggggccgact gctcgtgctg gtcttcgggt
     2881 ccacgaccat cgcggtcacc acggtgttgg ccgtgttcat gggcggcttg gcgatcggca
     2941 gcgcgctcgg gggacgggtc gtcgaccggg tgcgacggcc gctgctggcc tacgcggtga
     3001 tcgaagccgc cgtagcggcc tacgccctgg cgatcccctg gttgttgcac gccgtggtcc
     3061 cggtgtacca ggcggtgtgg cgggaactct cgacgtcgcc gctctcggtc atggccgcgc
     3121 gggtcggtct ggtgtccgcg atcctcgcgg tgcccacggt gctgatgggc gcgaccttgc
     3181 ccgtgatcag ccgggtcgtg gccgcgggcg cgacatccat cgcgggccgg gtgtccgcgt
     3241 tgtatacgct gaacaccgcc ggcgccattg ccggcgcggt gggcgccgga ttcctgctgc
     3301 tgcccgcggt cggggtgacc gcgacgatcg tggtcgcggc ggtcggcaac gcggcggccg
     3361 cgatcacggc gtgggcgttg gtgcggcgag gcttcgattc gacgtcccgg agtccgcgcg
     3421 tcgacgcggc cggctcggag tccggtgagg ccggcgtccg tcggcgcgtg gcggtgacgc
     3481 tcgcgatttc cggcgccgcc gcgctggtca acgaggtcgt gtggacgcgc gcgttgagcc
     3541 tggtgctcgg ctcgtcggtt tacgcgtttt ccgccatgct gacgaccttc ttgtccgggc
     3601 tggcggcggg cgcggcctgc gggacgtggc tcgtgcggcg gacctcgccg cggttcgcga
     3661 cgctgggcgc ggttcaactg gcgacggcgc tggcgtcatt ggtcacgctg gcgctgatcg
     3721 ggcggttgcc gttggccctg atcgccgcgg tcggatcgct ccggggactg ggggccgacg
     3781 tcatccccgc cgtgcagttc gcgttgtcgt ttctggtgat gttcctgccc acgttcgggt
     3841 tcggcgtggt gttccccgtg gcgctgtcgc tggcggtccg gagcccggat gcggtcggga
     3901 cgtcggtggg ccgcctgtat gcggccaaca ccgcgggggc gatcgcgggc gcggtggtcg
     3961 ccgggttcgt gttggtgccg ttggtgggcg tgccgggtag tctcgtcgcg gcggccgcaa
     4021 cctgcgcggt gttgggtctg tggctggtgg tcggagaccg atcgatcgcg cgacggctgg
     4081 cgtggggagg ggtcctcggt tcggccgtgc tcggggcgct ggtgctgggg gcgattccgc
     4141 cgtggagcgg gaccttgatg tcgtcggggg tgtactggaa tctcccccgg tatctggccc
     4201 tggcgga
//
LOCUS       DBNGTT010000345         4336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_336798, whole genome shotgun sequence.
ACCESSION   DBNGTT010000345 DBNGTT010000000
VERSION     DBNGTT010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_336798"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="AC16BC_07720"
     CDS             <1..553
                     /locus_tag="AC16BC_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3241547.1"
                     /translation="ARMTRVPGRFEKVDAGQDFTVVVDYAHTEDALQRVLAVARDLCR
                     GRLITVFGCGGDRDPGKRAPMGRVAARVSDLVILTSDNPRGEDPLAILSAIEQGLREE
                     LAARAPDRPSAFSRQPYLTLPDRREAIERAVQMAEAGDLIVIAGKGHEDYQIVGDRRL
                     SFDDRAAAREAIEQRLARRARSA"
     gene            577..1983
                     /locus_tag="AC16BC_07725"
     CDS             577..1983
                     /locus_tag="AC16BC_07725"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR3241548.1"
                     /translation="MFLVGDLAAAVRGRIVAGRADAVIESVSTDSRRLAPGALFWALP
                     GERFDGAAYVADALAAGACGAVVAAGSVPDPAVWRGRDAAIIAVDDPLRALQDAAAAY
                     RSRFAIPVVGVTGSNGKTTTKEMAAAILAQGGGVLKTQGNLNNHIGVPLTVFGLRPDH
                     RAAVVELGVNHHGEMTRLCEIARPTIGVITNIGHAHLEGFGGLEGVARAKGELFASLP
                     ADGMALLNADDPHVMALRGALRSESLTFGLGSGDVRGRMIDEPARTGTRVEIRYAGSV
                     IECVIPVVGRHNAANAVSAAAVGVSLGVDLAAVKRGLEAFRPATMRSELVTTASGIDV
                     FNDAYNANPSSMERALAAVGRLRGAGRVWAVLGEMRELGEASESLHRDVGRVAARAGL
                     DGLVAVGPLARWMADEAIKAGMPPRAIAWVETADEAVPVIVAWSRPGDVVLVKGSRRV
                     GLERVVSGLIAGAPSAAT"
     gene            1994..3070
                     /gene="mraY"
                     /locus_tag="AC16BC_07730"
     CDS             1994..3070
                     /gene="mraY"
                     /locus_tag="AC16BC_07730"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3241549.1"
                     /translation="MIYHLLYPWHTEYAFLNVFRYITFRTIYAVLTALVISFLFGPTI
                     IRWLQRLHIGQQIREEGPKSHQAKSGTPTMGGLLILIAVVCSTLLWADLAKRHVWVVL
                     LATVGFGLIGFWDDYLKVVKRHSRGLIPRYKFSLQVAVAAVSAGVLAMGPGFSTQIAL
                     PFVKTATVDLGWVYFVLVVLIVVGSSNAVNLTDGLDGLAIGPIIVAATAYMIVAYAAG
                     HRKIADYLLISYIDGAGELAVFCGAMIGASLGFLWFNTYPATVFMGDVGSLPLGAALG
                     LVAVVSKHELLLLLVGGLFVIEALSVIFQVASFKSRGKRVFLMAPIHHHFELKGWEEP
                     KVVVRFWIIAIILALLSLSTLKLR"
     gene            3081..>4336
                     /gene="murD"
                     /locus_tag="AC16BC_07735"
     CDS             3081..>4336
                     /gene="murD"
                     /locus_tag="AC16BC_07735"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3241550.1"
                     /translation="MDLKDKRVTVVGLARSGAAAARLLLRERAVVSVTDRRPPAELAE
                     WTKAFGPGDVRWFLGGHPDEAFLGADLIVLSPGVPVASPPLQAAKAKGIPIWSELELA
                     SRFVSAPISAITGSNGKSTTTALAAAMCGAAGRRTFIGGNYGVPLSEATLPGAAWGEV
                     VVEVSSFQLETVTTFHPVVAALLNVTPDHLDRYPNVAAYAAAKFRLFEHQGRDDAAVL
                     NADDPVCVDPAFTARLAARRVSFSRRSAPSGDAVWVDNGAITYRMGERNGRVLEVDRL
                     RIAGVHNVENALAATAVALSRGIDPAVIATVLSEFRGLEHRMELVRDLRGVTYINDSK
                     GTNVGAVQKSLESVSRPIILIAGGVGKGADFSALRPLVMRRVKKAVLIGQARPQLRQA
                     FEGITTVAEANGMEEAVRIAADSAVPG"
BASE COUNT          613 a         1323 c         1661 g          739 t
ORIGIN      
        1 cgcccggatg acccgggtcc cgggccgatt cgaaaaggtc gacgccggac aggacttcac
       61 cgtggtcgtg gattacgcgc acacggagga cgcgttgcag cgggtgctgg cggtggcgcg
      121 cgacctgtgc cgcggccggt tgatcacggt gttcgggtgc ggcggcgacc gcgatcccgg
      181 gaaacgggcg cccatgggcc gggtggccgc gcgcgtgagc gacctggtca tcctcacctc
      241 ggacaatccg cgcggcgaag accccctggc gatcctgagc gcgatcgagc aggggcttcg
      301 cgaggaactc gccgcgcgcg cgccggatcg accgtcggcg ttttcgcgac aaccgtattt
      361 gacgttgccc gatcggcgcg aggcgatcga acgggcggtg cagatggccg aagccggcga
      421 cctgatcgtg atcgcgggca aagggcatga ggattaccag atcgtgggcg atcggcggct
      481 ctcgttcgac gatcgcgcgg cggcgcggga agcgatcgaa caacggctcg cgcgccgagc
      541 gcggtccgcg tgagcccggc gtcggagtgt cgcggcatgt ttctcgtcgg tgatcttgcg
      601 gccgcggtgc ggggtcggat cgtggcgggc cgggccgacg ccgtgatcga gtccgtttcc
      661 acggactcgc ggcggttggc gccgggcgcg ttgttctggg cgctccccgg cgagcgcttc
      721 gacggggccg cgtacgtggc ggacgcgctc gccgcgggcg cgtgcggcgc ggtcgtggcc
      781 gcgggctcgg tgccggaccc ggcggtgtgg cgagggcgcg acgcggcgat catcgcggtg
      841 gacgaccccc tgcgcgcgct gcaggacgcc gccgcggcgt accggtcgcg gttcgcgatt
      901 ccggtggtcg gcgtgaccgg cagcaacggc aagaccacca ccaaggagat ggcggcggcg
      961 attctggcgc agggcggcgg cgtgctcaaa acgcagggca atctcaacaa ccacatcggg
     1021 gtgccgctca cggtgttcgg cctccggccc gaccaccgcg ccgcggtggt ggaactcggc
     1081 gtcaatcacc acggggagat gacgcggttg tgcgagatcg cgcggcccac gatcggcgtg
     1141 atcaccaaca tcggccacgc ccatctggag gggttcgggg gactggaggg cgtggcgcgc
     1201 gccaagggcg agttgttcgc ctccttgccc gcggacggga tggcgttgtt gaacgccgac
     1261 gacccccacg tgatggcgct ccgcggggcg ctgcggagcg agtccctgac cttcggcctc
     1321 ggaagcggcg acgtgcgggg acggatgatc gacgaacccg cgcggacggg cacgcgggtg
     1381 gagatccggt acgcggggtc ggtgatcgag tgcgtcatcc ccgtggtcgg ccgtcacaac
     1441 gcggccaatg ccgtgtccgc ggccgcggtg ggggtgtcgt tgggcgttga cctggccgcg
     1501 gtgaagcgcg ggctggaagc gttccggccc gcgaccatgc ggagcgagct ggtgaccacg
     1561 gcgtcgggca tcgacgtgtt caacgacgcg tacaacgcca acccgtcatc gatggagcgc
     1621 gcgttggccg cggtggggcg gttgcgcggg gcggggcgcg tgtgggcggt cttgggcgag
     1681 atgcgggaac tgggcgaggc gtccgaatcg ttgcatcgcg acgtggggcg ggtggcggcc
     1741 cgcgcggggc tcgacgggct ggtggcggtc ggcccgttgg cgcggtggat ggccgacgaa
     1801 gcgatcaagg ccgggatgcc gccgcgcgcg atcgcgtggg ttgaaaccgc ggacgaagcg
     1861 gtgccggtga tcgtggcgtg gagtcgtccg ggggacgtgg tcctggtcaa ggggtcgcga
     1921 cgcgtcgggt tggagcgcgt ggtgagcggg ctgatcgcgg gcgcaccgtc ggccgcgacc
     1981 taacggagtc gggatgatct atcacttgct gtatccgtgg cacaccgaat acgcgttcct
     2041 caacgtgttt cggtacatca cgttccgaac catctacgcg gtcttgaccg cgctggtgat
     2101 cagttttctg ttcggcccca ccatcatccg ctggctgcag cggctgcaca tcggacagca
     2161 gatccgcgaa gaggggccca agtcgcatca ggccaagtcg gggacgccca ccatgggcgg
     2221 gttgttgatc ctgatcgccg tggtctgttc caccctgctg tgggccgatc tggccaaacg
     2281 ccacgtctgg gtggtgctgc tcgccaccgt gggattcggt ctcatcggat tttgggacga
     2341 ctacctcaag gtcgtgaagc ggcactcgcg cggcctcatt ccccggtaca agttttcgct
     2401 gcaggtggcg gtggccgccg tgtccgccgg ggtgctggcg atgggtccgg gtttttcaac
     2461 gcagatcgcg ctgccgttcg tcaagacggc caccgtggat ctgggctggg tctatttcgt
     2521 attggtggtg ctgatcgtgg tcggatcgtc caacgcggtc aacctgaccg acggtctcga
     2581 cggcctcgcg atcggcccga tcatcgtggc ggcgaccgcg tacatgatcg tggcgtacgc
     2641 cgctgggcac cgcaagatcg cggactacct cttgatctcc tacatcgacg gcgcgggaga
     2701 actcgcggtc ttttgcggcg ccatgatcgg cgcgagtttg ggtttcctgt ggttcaacac
     2761 ctatccggcg accgtgttca tgggcgacgt ggggtcgctc ccgctgggcg cggcgctggg
     2821 cctggtcgcg gtggtctcca aacacgaatt gttgctgtta ttggtcggcg gcctgttcgt
     2881 gatcgaagcg ctttccgtga tctttcaggt cgcgtcgttc aagtcgcgcg ggaaacgcgt
     2941 gttcctgatg gcgccgatcc accaccattt cgaattgaag ggatgggagg aaccgaaagt
     3001 cgtcgtccga ttctggatca tcgcgatcat cttggccctg ttgagtctca gtaccctgaa
     3061 attgcggtaa ggggcattgg gtggacctca aagacaaacg ggtgacggtc gtggggttgg
     3121 cgcgaagcgg cgccgccgcg gcgcggttgc tcctgcgcga gcgcgcggtg gtgtcggtga
     3181 ccgatcgacg tccgcccgcg gagctggccg agtggaccaa ggctttcggg cccggggacg
     3241 tccggtggtt tctggggggc catcccgacg aagcctttct gggcgcggat ttgatcgtgc
     3301 tgagtcccgg ggtcccggtg gcgagcccgc cgctccaggc ggccaaggcc aaggggattc
     3361 cgatctggag cgagttggaa ctggcgtcgc gattcgtgtc cgcgccgatc agcgcgatca
     3421 ccgggagcaa cgggaagagc accaccaccg cgctggccgc ggcgatgtgc ggagccgcgg
     3481 gccggcgcac cttcatcggg ggcaactacg gcgtgccgct ctccgaggcc accctgcccg
     3541 gggcggcgtg gggcgaggtc gtggtggagg tgtccagctt ccaattggag accgtgacga
     3601 cgtttcatcc cgtggtggcg gcgttgctga acgtgacccc cgatcatttg gaccgatatc
     3661 cgaatgtcgc ggcctacgcc gccgcgaagt tccggctctt cgaacaccag gggcgggacg
     3721 acgcggcggt gctgaacgcg gatgatcccg tgtgcgtgga ccccgcgttc accgcgcggc
     3781 tcgccgcccg acgcgtgtcg ttctcccggc gttcggcgcc ctcgggcgac gcggtgtggg
     3841 tggacaacgg cgcgatcacc taccggatgg gcgagcgcaa cgggcgcgtc ctggaagtgg
     3901 accgcctgcg gatcgcgggg gtgcataacg tggagaacgc gctggcggcg accgccgtcg
     3961 ccctgtcgcg cggaatcgat cccgcggtga tcgcgaccgt gctgtcggag tttcgcgggc
     4021 tcgaacatcg catggagctg gtccgcgacc tgcggggggt cacgtacatc aacgattcga
     4081 aggggacgaa cgtgggcgcg gtccagaagt cgctggagag cgtgagccgt ccgatcatcc
     4141 tgatcgcggg cggggtcggc aaaggcgcgg atttctccgc gttgcgtccg ctcgtgatgc
     4201 ggcgggtcaa gaaagcggtc ctgatcggcc aggcgcgacc gcagctccgg caagccttcg
     4261 aggggattac cacggtcgcg gaggcgaacg ggatggagga ggcggtgcgg atcgcggccg
     4321 acagcgcggt cccggg
//
LOCUS       DBNGTT010000346         3787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3276253, whole genome shotgun sequence.
ACCESSION   DBNGTT010000346 DBNGTT010000000
VERSION     DBNGTT010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3276253"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="AC16BC_07740"
     CDS             <1..937
                     /locus_tag="AC16BC_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3241551.1"
                     /translation="SSWLDQKSARLAQEEKQESARQRALKHELEWVRTAPKGRHAKSK
                     ARIKAYEELASQEFTSRGETKDIMIPPGPRLGELVIEVKDLVKGFRDRLLIDGLSFSL
                     PRGGIVGIIGPNGAGKTTLFRMLVGQDKPDSGTIKVGDTVKLAYVDQSRDTLDGAKTV
                     FDEISGGHDVIMIGKRELPARAYVGGFNFKNTDQQKLVKELSGGERNRVHLAKLLKSG
                     GNVLLLDEPSNDLDVETLRAVEEALLDFAGCVLVISHDRWFLDRIATHILAFEGDSRV
                     VWFEGNYLEYEADRHKRLGSEADQPHRIKYRPLTR"
     gene            954..1139
                     /locus_tag="AC16BC_07745"
     CDS             954..1139
                     /locus_tag="AC16BC_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241552.1"
                     /translation="MKMLKVSRGSLFLCETCYHIIEESGQVEGDRASSDAVIAELCPN
                     CEDQNKPLLDDLLGSEE"
     gene            1235..1870
                     /locus_tag="AC16BC_07750"
     CDS             1235..1870
                     /locus_tag="AC16BC_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3241553.1"
                     /translation="MRITEIFHSIQGESTYAGLPCVFVRVTGCPLRCTWCDTAYAFYD
                     GEDLSLDQIIERVASFGCRLVEITGGEPLHDPEAFPLITRLADAGYTVLVETSGAIDI
                     TPVDRRAVIIMDLKCPGSGMADRNLWSNLDRLKPGDQVKFVLKDREDYLWARDVLART
                     RVHERQTVLLSPVFGELDAKLLAEWMLEDRLSARLQVQLHKLIWDPATRGV"
     gene            2038..3618
                     /locus_tag="AC16BC_07755"
     CDS             2038..3618
                     /locus_tag="AC16BC_07755"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR3241554.1"
                     /translation="MTRPITVKTGSIRQATEDAVVVFRNQGGEAPEFFAAIGRAVGPS
                     FAEALAGAGFEGKAGQAVLVHTAGRIKPRVVVAAGLGPREGMGPERVRQAAARAAQRA
                     RDAGCERVAFDAAIGDDASSTVRACVEGALMGLYRFTRYKTAHDNNGRAVKTLVCYIG
                     SGDAAAAQAGAQRGAIVAESVNHVRDLGNLPANVATPTYIADRARAVARANKLTCEVI
                     DRRGMERLGMGALLGVAKGSMEPPKLIVLRYDGGKRVAGAAERPVALVGKTITFDSGG
                     ISLKPADKMEQMKSDMMGGAGVLSVMEAAARLALPISIVGLLPATENMPSGTAIKPGD
                     VVTAMSGTTVEVINTDAEGRLVLADALAYAVKRYQPRLIVDIATLTGACSVALGQHAI
                     GLLGTDAALIARLQRAAEASGERVWQLPLWEEYYDQIKSDVADLKNTGGRPAGTITAA
                     AFLSKFVGQTPWAHLDIAGTMWSDESRHYVTKGPTGAGVRLLIQFLMDEVGRSGARSI
                     RGRAPARSRTTARRVARR"
BASE COUNT          686 a         1155 c         1358 g          588 t
ORIGIN      
        1 ctcgtcctgg ctcgatcaga aatcggcccg gctcgcgcag gaagaaaaac aggaatccgc
       61 gcggcagcgc gcgctcaaac acgagttgga atgggtgcgc accgcgccca agggacgcca
      121 cgccaagagc aaggcgcgta tcaaagccta cgaggaactc gcgtcgcagg aattcacgtc
      181 gcgcggcgaa accaaggaca tcatgatccc ccccgggccg cgcctgggcg aactggtgat
      241 cgaggtcaag gatctggtca agggattccg cgaccgattg ttgatcgacg gcctgagttt
      301 ttcgctcccg cgcggcggga tcgtggggat cattggtccc aatggcgcgg gcaagaccac
      361 gctcttccgg atgctggtgg ggcaggacaa gccggacagc gggaccatca aagtcggcga
      421 caccgtgaag ctggcgtatg tggatcagtc ccgcgacacc ttggacggcg ccaagaccgt
      481 gttcgacgaa atttccggcg gccacgacgt gatcatgatc ggcaaacgcg agttgcccgc
      541 gcgcgcgtac gtgggcgggt tcaatttcaa gaacacggat caacaaaagc tcgtgaagga
      601 gctctcgggg ggagagcgca accgcgtgca cctggccaaa ttgctcaaga gcggcgggaa
      661 cgtgttgctg ctggacgagc cgagcaacga cctggacgtg gaaaccctgc gcgcggtgga
      721 agaagcgctg ctggactttg ctggctgcgt gctggtgatc agccatgacc gctggttcct
      781 ggaccggatc gccacgcaca tcctggcgtt cgagggcgac agccgggtgg tgtggttcga
      841 gggcaactat ctggagtacg aggcggatcg tcataagcgc ctcggctcgg aagcggacca
      901 gccgcaccgg atcaagtacc gcccgctcac ccgctgaccg gatcgggcgc gccgtgaaga
      961 tgctcaaggt cagtcggggg tcgctgtttc tctgcgagac ctgctaccac atcatcgagg
     1021 agtcggggca ggtcgagggc gaccgcgcca gctccgacgc ggtgatcgcc gagttgtgcc
     1081 ccaactgcga agaccagaac aaaccattgc tggacgactt gctgggcagc gaggaatagg
     1141 accgacgatc cgatcccgat ttcttgattc actcccggtc tcttgctatt ctgactcact
     1201 tgtcgaaggc gtcaggacgc cacgccgggc caccatgcgc attaccgaga tcttccacag
     1261 catccagggc gagtccacct atgcgggcct cccgtgcgtg ttcgtgcggg tgaccgggtg
     1321 tccgctgcgc tgtacctggt gcgacaccgc gtacgcgttt tacgacggcg aagacctgag
     1381 cctggatcag atcatcgagc gcgtggcgag cttcggctgt cgattggtgg agatcacggg
     1441 cggcgagccg ctgcacgatc ccgaggcgtt ccccctgatc acgcgcctgg cggacgcggg
     1501 gtatacggtg ctggtggaaa ccagcggcgc catcgacatc acgccggtgg accgtcgggc
     1561 cgtgatcatc atggatctga agtgccccgg cagcggcatg gcggatcgca atctctggag
     1621 caatctcgat cgcctcaagc caggcgacca ggtaaagttc gtgctcaagg accgggagga
     1681 ttatctgtgg gcgcgtgacg tgctggcgcg cacgcgggtg cacgagcggc agaccgtgtt
     1741 gctctcgccc gtgttcggcg aactggatgc caagctgctc gcggaatgga tgctggagga
     1801 tcgcctgtcg gcgcgactcc aagtgcaatt gcacaaactg atttgggatc ccgcgacacg
     1861 cggcgtatag cgtaagaacg tgtccgagtc accgcgaaag cggagcctgt ccaggaaagc
     1921 cagacgcaag gcgccgctga gcaccggagc ggagcgtaca gggtgcgtac gtgagcgccg
     1981 gagcgcaagc ggcaacgccg cagatggctt tcctggacag gctcctagag gagggatatg
     2041 acgcgaccga tcacagtgaa aaccgggtcg atccggcaag ccacggaaga cgcggtggtg
     2101 gtgttccgca accagggggg ggaggctccc gagttcttcg ccgccatcgg ccgggcggtg
     2161 ggcccgtcgt tcgcggaggc attggccggc gccggattcg agggcaaggc tgggcaagcg
     2221 gtcctggtgc acaccgcggg tcgcatcaag ccgcgcgtgg tggtggcggc ggggctgggt
     2281 ccgcgcgagg gcatggggcc cgagcgcgtg cggcaggccg cggcgcgcgc ggcgcaacgc
     2341 gcgcgcgacg cggggtgcga gcgcgtggcg ttcgacgccg cgatcgggga cgacgcatcg
     2401 tcgacggttc gcgcctgcgt cgaaggcgcc ctaatgggat tgtatcgatt cacccggtac
     2461 aagaccgcgc acgacaacaa cgggcgcgcg gtcaagacgc tggtctgtta tatcgggtcg
     2521 ggggacgcgg cggccgcgca ggctggcgcg caacgcggcg cgatcgtggc ggagagcgtg
     2581 aaccacgtcc gcgacttggg gaacttgccc gcgaacgtgg ccactcccac ctatatcgcg
     2641 gaccgggcgc gggccgtggc gcgggcgaac aaattgacgt gcgaggtgat cgaccgcagg
     2701 ggcatggagc gcctgggcat gggggctttg ctgggcgtgg ccaagggcag catggagccg
     2761 ccgaaactca tcgtcctccg gtacgatggc ggcaaacgcg tcgcgggagc cgcggagcgt
     2821 cccgtcgccc tggtcggcaa gaccatcacg ttcgacagcg gcggcatttc gctcaagccc
     2881 gcggacaaga tggagcagat gaagagcgac atgatgggcg gcgcgggcgt gttgtcggtg
     2941 atggaggccg cggcgcggct ggcccttccg atctcgatcg tcgggctctt gcccgcgacc
     3001 gaaaacatgc cgagcgggac cgcgatcaaa cccggcgacg tcgtgaccgc catgtccggc
     3061 accacggtcg aagtgatcaa caccgacgcg gaggggcgat tggtcctcgc ggatgcgttg
     3121 gcctacgcgg tcaagcggta ccagccgcgc ctgatcgtgg acatcgccac gctgaccggc
     3181 gcgtgttcgg tcgcgttggg ccagcacgcc atcggattgc tgggcacgga cgcggcgctg
     3241 atcgcgcgcc tacagcgcgc cgcggaggcc tccggggagc gggtctggca gttgcccttg
     3301 tgggaggagt actacgacca gatcaagagc gacgtggcgg accttaagaa cacgggcggc
     3361 cgacccgcgg ggaccatcac ggccgcggcg tttttgagta agttcgtggg ccagaccccg
     3421 tgggcgcacc tggacatcgc gggcacgatg tggagcgacg agtcgcggca ttacgtgacc
     3481 aaaggcccca ccggcgcggg cgtgcgcctg ctcattcaat ttctgatgga tgaggtcggt
     3541 cgatccggcg cgcggagtat ccgcggccgg gctccggcgc gatcgcgcac cacggcccgt
     3601 cgcgtggccc gccgatgaac ctccgcgaaa aactggccca gctcatcatg gtcggattcg
     3661 agggcgccaa gcccggaccg gccatcacgc gtctggtgaa gaccacgcgg gtcgggggcg
     3721 tgatcctgtt ccggcgcaac gtggagaccc ccgcgcaggt ggcgtcgctc acgcgacgtc
     3781 tgcagcg
//
LOCUS       DBNGTT010000347         9158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1743925, whole genome shotgun sequence.
ACCESSION   DBNGTT010000347 DBNGTT010000000
VERSION     DBNGTT010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1743925"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="AC16BC_07760"
     CDS             <1..1098
                     /locus_tag="AC16BC_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3241555.1"
                     /translation="MKDTAKRDSVGFIFNFASNSKEYAAMKLTLFLSAGAVVALLGIF
                     LTYVGTGLNTFNLIELQGHEQQFSPVMRSAIWLMLFFGFASIAPVWPMHSGSPVGHAA
                     APAAVSMLHAGVLMKLGHFSIIRVGYWLFPQTTAEWAWLIAILCVGSIVYGGLVAFFQ
                     RDTKYVIGYSSSSHMGYIFLGMAALNMISMTGAVLYMFAHALATGMLFSMAGWVYDQT
                     HTRDIPSLGGLGRKMPFIMGAFVVGLMASVGLPGTVNFIGELMVFVGSWEQYPVQVVI
                     AVIGITITLGYLLRMFIGLFLGDMDPKWAHAADAKWVDRVPILIMVSFSLFFGIFPTQ
                     FIDVISAGMYPIVEQIRSVSVAATSIVGGGG"
     gene            1102..2568
                     /locus_tag="AC16BC_07765"
     CDS             1102..2568
                     /locus_tag="AC16BC_07765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3241556.1"
                     /translation="MEFTLAFSWSDLLLLLPELLLTALIGVVLVVDFTFPKVSKNTLA
                     WVTVAGLCAVLASLAWMWGDNVQGALFKQTFVLDPFAVFFKIIVVLATILIVLASLDY
                     IKRIVLFRGEYYIMVLLSALGMLFMASSNDLLTMFITIEFSTFGFYILVAYLREDNLF
                     SSEAGLKFFVLSVFTAAFMVFGISLIYGETGTIIFPEIAAAKPVLTGGLVIGFLCLIV
                     GLGFKIGAFPFHNWIPDIYQGAPTPITAYLGIAPKAAALAIVLRVFFSTFGQFKPEWV
                     WLLIGMAALSMTYGNVVAIAQTDIKRMLAYSGIAQMGNILIGLAAGTKMGGDSVLFYL
                     LAYLMANVGAFAVIIIFSNLTGSDRIEDYRGLARRAPLLAASMLVFLLSLAGVPPFAG
                     FIAKIYVFAAAMKQGLIALVCVGLVNIVISMYYYIVIIKKMYINEPVDRRPVPMSASM
                     QAVVFATLAGTVILGIYPGPFIEWAVSSTSLFASLVTP"
     gene            complement(2606..2914)
                     /locus_tag="AC16BC_07770"
     CDS             complement(2606..2914)
                     /locus_tag="AC16BC_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241557.1"
                     /translation="MNLDIWAAAAGPGFAPVVYLNGTLLFIAGLAIVRAHNHWTGGWP
                     VLVTLTGWCALLFGLIRMIAPVSAQRAGENHTALYALLVVLLAIGVFLTVKGYGRADS
                     "
     gene            3444..4220
                     /locus_tag="AC16BC_07775"
     CDS             3444..4220
                     /locus_tag="AC16BC_07775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3241558.1"
                     /translation="MTAIDRWWVVVASILAMLLTACQTVPLTGRHQLIIVSEEEELGL
                     GAAAYKKTLTSSKLSTDTATTAMVRRVGERIAKAADRPDYKWEFNLVEDKTPNAFCLP
                     GGKVVVYTGILPFTKDETGLAVVMGHEVAHALARHGAERMSTGELAGIAGKTAAIIFG
                     GGNPESEKQVEQAFGIGSNVGVLLPFSRHQESEADEIGLMLMAKAGYDPRQAVDFWTR
                     MAQGQNGAPPEFLSTHPSDQTRIKRIEGWIPEALKSYQPR"
     gene            complement(4274..6829)
                     /locus_tag="AC16BC_07780"
     CDS             complement(4274..6829)
                     /locus_tag="AC16BC_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR3241559.1"
                     /translation="MRRPLIPFALAMAVGIALGALADGFPITAAALALVVIAGTGLGR
                     RKLPRAVSFGPVHWAVILAAAAYTLATPPPAPDPRLTSEPVTVDAVLDEPVRHMTDWT
                     SVTASDLRLAGKKPDDLRGRIKLSGPPDALAALRYGDRIRVTVQFRPPRGFQNPGLFD
                     YGRWLERQGVIAVATVKPGEIARTGHRDSPVFDRVYEWRERIRVAAVASLPADVAPIF
                     LAMVTGETGYLTSDLRDRFMASGTVHLLSISGSHLGLIALIVFAAVRLIVLRLPERLL
                     LRVTMRVMPSQIAAAATMIPVVFYALLAGAQVATMRALLMILVYLSAVLIHRHGDLLN
                     LLAFAAIALLAWDPHALTDVSFQLSFLSVWCIALALEWSSGHEFSPEPPGPVRWQDRL
                     RARASALTLTSVAAGVGTAPLVAYHFFQVNWVGVIANPIIIPLAGALVVPLGLMSGIA
                     AALTDSPTLPLAEWNAAALHALLRVVDFFAAWPLSTVHVAAPSIAVLGLWYLIMASLV
                     DRRVKPWKRGLAGGALVILAVPALVGIPNWKSSATLEVTILDVGQGDSTVVRFPSGRV
                     MVVDAGKRFFELDTGRLVVAPTLWNQNVRHIDYLVATHPQLDHIGGLLFVADRFTVGE
                     AWINGRRPDSWAAREFDAVLARRGVQTNVIPREQPLWVDAARIWRVNPLAESPFAPLP
                     NRATENDRSIVLRVEYGRASFLLTGDVEFEGERAMTEANPQWNLLRSTVLKVPHHGSR
                     GALHPVFLRAVAPRISVVSVGATNTYGHPAPQTLRAYRRLNTRLFRTDEDGAIKLVTD
                     GTRLDVFRYDDLTIQPVRWGHGLAAAEWRNLRTALGAPTPSLSLDLTSTEPPL"
     gene            complement(6901..7608)
                     /locus_tag="AC16BC_07785"
     CDS             complement(6901..7608)
                     /locus_tag="AC16BC_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3241560.1"
                     /translation="MKRLCAALLLAAATQANPAAAADPHEAVINSPHNVFARSEFPIG
                     RRVCAGCHAEGAGAPAAESPAQPDEALAPEGESAPPPPLWQRSAAPYRIYSATRPAAA
                     SVGNGSDACLECHDGVLGQEIHASLVRNGKTFDHPSNVVYPRLPDGRFVPKTPTANQY
                     RYWSIPDLRDGNLISPTGPSSAVLAASDPAAAALRSVRTSDGMVQCGSCHDPHDNGSA
                     PFLRAPAQDLCFVCHDR"
     gene            complement(7605..9002)
                     /gene="glmM"
                     /locus_tag="AC16BC_07790"
     CDS             complement(7605..9002)
                     /gene="glmM"
                     /locus_tag="AC16BC_07790"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3241561.1"
                     /translation="MSKLGVERRLFGTDGVRGTANIEPVTAETAMKLGRAAAYLFKKH
                     HTGHHRFVIGKDTRLSGYMIESALTAGVCSMGVDVLLVGPMPTPAIAFLTKSLRADAG
                     VVISASHNPFEDNGIKFFSHDGLKLPDSVEQEIEDLIFSGAIEHERPTAREIGKAFRV
                     DDADGRYIEFVKNTLPKGMQFDGMKIVVDTANGAAYKVTPMVLRELGAEVITIADRPN
                     GTNINQHCGALYPEALQKAVAMHHADLGIAHDGDADRVVFCDDQGKIVHGDQILALCA
                     LELERVGRLAGHTVVATVMSNLGLELALRHAGISLVRTAVGDRYVLERMLKDGYVFGG
                     EQSGHIIFLDYNTTGDGLITAVQVLATMERTGKRLSELASCMAVCPQVLVNVRAAERR
                     DLNTVPGYAALVSNFERRLGPQGRLLIRYSGTEPLLRVMVEGEDFQTISTVAEELAET
                     LRTLVGAPSAPMGAA"
BASE COUNT         1589 a         2868 c         3050 g         1651 t
ORIGIN      
        1 atgaaggaca cggccaagcg cgactccgtg ggcttcattt tcaacttcgc gtccaacagc
       61 aaagagtacg cggccatgaa gctgacgttg ttcctgtcgg ccggggccgt ggtggcgttg
      121 ctgggcatct tcctcacgta cgtgggtacc gggctgaaca cgttcaacct gatcgagctg
      181 cagggccatg agcagcagtt ttcgcccgtg atgcgaagcg cgatctggct catgctgttc
      241 ttcggcttcg cgtcgatcgc gcccgtgtgg cccatgcact cggggtcgcc ggtgggccac
      301 gcggccgcgc ccgccgcggt gtccatgctg cacgcgggcg tgttgatgaa gctggggcac
      361 ttctccatca tccgcgtggg gtactggttg ttcccgcaga ccacggccga atgggcgtgg
      421 ctgatcgcca tcctgtgcgt ggggtcgatc gtctatggcg ggctggtcgc gttcttccag
      481 cgcgacacca agtacgtgat cgggtattcc agctccagcc acatggggta catcttcctg
      541 ggcatggccg cgctcaatat gatcagcatg accggcgccg tgctctacat gttcgcgcac
      601 gcgctcgcca ccggcatgct cttctccatg gcgggctggg tgtacgacca gacgcacacc
      661 cgggacatcc cctcgctcgg cggcctcggc aggaagatgc cgttcatcat gggcgcgttc
      721 gtggtggggc tcatggcgtc ggtgggactt ccgggcacgg tcaacttcat cggggaactg
      781 atggtctttg tcgggagctg ggagcaatat ccggtgcagg tggtgatcgc ggtgatcggc
      841 atcaccatca cgctcgggta cctgctgcgc atgttcatcg gcttgttcct gggcgacatg
      901 gaccccaagt gggcgcacgc cgctgacgcc aagtgggtgg accgcgtgcc gatcttgatc
      961 atggtctcgt tcagcctctt tttcgggatc ttccccacgc agttcatcga cgtgatcagc
     1021 gcggggatgt atccgattgt ggaacagatc cgctcggtga gcgtggcggc cacgtctata
     1081 gttggaggcg gcgggtaggc gatggagttt acgctcgcat tttcatggag tgatcttctc
     1141 ctcctgctgc cggaactgtt gctcacggcg ctgatcggcg tggtcctggt ggtggacttc
     1201 acgttcccca aggtcagtaa gaacacgctc gcgtgggtga cggtcgccgg gttgtgcgcg
     1261 gtgctcgcga gcctggcgtg gatgtggggc gacaacgtgc agggggcgct gttcaagcag
     1321 acgttcgtgc tcgacccgtt cgcggtgttc ttcaagatca tcgtggtgct ggccacgatc
     1381 ctgatcgtgc tggcctcgct cgactacatc aagcgcatcg tgttgttccg cggcgagtac
     1441 tacatcatgg tcctgctgtc cgcgctgggg atgctgttca tggcgtcctc caacgacctg
     1501 ctgaccatgt tcatcaccat cgagttttcc acgttcgggt tctacatcct ggtggcgtac
     1561 ctgcgtgaag acaacctgtt ctcgagcgaa gcgggcctga agttcttcgt gctgagcgtg
     1621 ttcaccgcgg cgttcatggt gttcggcatc agcctgattt acggcgaaac cgggaccatc
     1681 atcttcccgg agatcgcggc cgccaagccc gtgttgaccg gcggcctggt catcgggttc
     1741 ctgtgtctga tcgtgggatt gggctttaag atcggcgcgt tcccgtttca caactggatc
     1801 cccgatatct accagggcgc gcccacgccg atcaccgcgt acctgggcat cgcgcccaag
     1861 gctgcagcat tggccatcgt gctgcgtgtg ttcttttcca cgttcgggca gttcaagccc
     1921 gaatgggtgt ggctgctgat cgggatggcg gccctgtcca tgacttacgg caacgtcgtg
     1981 gccattgcgc agaccgacat caaacgcatg ctcgcgtatt ccggcatcgc gcagatgggc
     2041 aatatcctga tcggcctggc cgcgggcacc aagatgggcg gggattcggt gctcttttac
     2101 ctgctggcgt atttgatggc caatgtgggc gcgttcgcgg tgatcatcat tttctcgaat
     2161 ctcacgggca gcgaccggat cgaagactat cgcgggctgg cccgccgcgc gccgctcctg
     2221 gccgcgtcca tgctggtgtt cctgctctcg ctggccggcg tccctccgtt cgcgggcttt
     2281 atcgccaaga tctacgtgtt cgccgcggcc atgaagcagg gcttgatcgc cctggtgtgc
     2341 gtgggcctgg tcaacattgt gatctcgatg tactactaca tcgtgatcat caagaagatg
     2401 tacatcaacg agcccgtcga ccgccgcccg gtgcccatgt ccgcgtccat gcaggccgtg
     2461 gtcttcgcca cgctcgcggg caccgtgatc ctcggcatct accccggccc gttcatcgaa
     2521 tgggcggtct cctccacgtc gctcttcgcg tccctggtca caccgtaagt ccggtcaccg
     2581 actcccagcg ctgccgcgca accgtctaag aatccgctcg tccataccct ttgacggtca
     2641 ggaagacccc gatcgccagg agcacgacga gcaacgcgta caaggcggtg tgattctctc
     2701 cggcccgctg agcggatacc ggagcgatca tgcggatcaa accaaaaagc agagcacacc
     2761 acccggtgag agtcaccagc acgggccagc cgcctgtcca atgattgtgc gcgcgaacaa
     2821 tcgcaaggcc ggcaataaag agcaacgtgc cgttgagata cacgacaggg gcaaaccccg
     2881 gcccggcagc ggctgcccag atatcaagat tcatcgcttc ggttatcgag agcgcgatga
     2941 gcgtagggcc gagtagtccc gcgatgcgtt ttgagttcgg cattttagcc tttataaggc
     3001 gaaaataaat ccgtccccgg ttgtgctgtt tctgatctcc atcgtgcttc cgaccaaatc
     3061 tcaaaggcaa ataatagaga tgcggtaatc aaacaaaata tggtagccca aaatggaaag
     3121 gcattggcaa aagcatttac tagagcgccg aagagagcgc caaacgctga ccaaagaatt
     3181 gggttggcca tgacgcagtc ccccgtgatt gaatttgaaa gcgccaccct agcatccacg
     3241 aaattcggtg tcaaagttct atatcgcgaa gcagctaaat cgcttctgtg ataaccccgt
     3301 catgatcgtg tttcggaaga aaacagatgc gggattttac ggtatcccct ggtgctgcgt
     3361 agtatcgtga tgacgccgga cgccgattcg cttgagccct gccatccccc tgtgccataa
     3421 tgcgtcgtcc gaatggagtc gggatgactg cgattgaccg atggtgggtg gtggtggcga
     3481 gtattctggc gatgttgttg accgcctgcc agaccgtgcc gctcaccggg cggcatcagt
     3541 tgattatcgt gtccgaggag gaggaactgg gcctcggcgc ggcggcgtac aagaaaaccc
     3601 tcacgtcgtc caagctcagc acggacacgg caaccaccgc catggtccgc cgcgtgggcg
     3661 agcggatcgc caaggccgcg gatcgccctg actacaagtg ggagttcaac ctggtcgaag
     3721 acaagacgcc caacgcgttc tgtctgccgg gcggcaaggt cgtggtgtac accggcattc
     3781 tgccgttcac caaggatgaa accggattgg ccgtggtcat gggccatgag gtcgcgcacg
     3841 cgctggcgcg ccacggcgcg gagcggatgt ccacggggga gttggcgggg attgccggga
     3901 agaccgcggc catcatcttc gggggcggca accccgaaag cgagaagcag gtcgagcagg
     3961 cgttcggcat cggcagcaac gtgggcgttc tgctcccgtt cagccggcac caggaatcag
     4021 aggccgatga gatcggtctg atgttgatgg ccaaggccgg gtacgacccg cgccaggccg
     4081 tggacttctg gactcgcatg gcgcaaggcc agaacggcgc gcccccggaa tttctctcca
     4141 cccaccccag cgatcagacc cggatcaagc gcatcgaagg ctggattccc gaggcgctca
     4201 agagttacca gcctcggtaa ccacaccggt tgttttgcgc ctatcgtagg ggcacggcat
     4261 gccgtgcccc tacctacagc ggcggttcgg tgctcgtcag atccaaagac agtgacggag
     4321 tcggcgcgcc gagcgcggtg cggaggttgc gccattccgc ggcggccagg ccgtgacccc
     4381 agcggaccgg ttgaatggtc aggtcgtcgt aacgaaacac atccagccgc gtgccgtcgg
     4441 tgacgagttt gatggcgccg tcctcatcgg tccgaaagag tctggtgttg aggcggcgat
     4501 aagccctaag cgtttgcggg gcggggtggc cgtaggtgtt ggtcgcgccc accgacacca
     4561 cgctgattcg gggcgcgacc gcccgcaaga acacggggtg caacgcgccc cggctgccgt
     4621 ggtgcggcac cttgagcacg gtgctgcgca gcaggttcca ctgcgggttg gcctcggtca
     4681 tggcccgctc gccttcgaac tccacgtcgc cggttagcag gaacgacgcg cggccgtact
     4741 ccacgcgcag cacgatcgac cggtcgtttt ccgtggcccg gttcggcaag ggcgcgaagg
     4801 gcgattccgc cagggggttc acgcgccaga tcctggccgc gtccacccac agcggctgct
     4861 cgcgcgggat gacattggtc tgcacgccgc gccgggcgag caccgcgtcg aactcgcgcg
     4921 ccgcccaact gtcgggcctg cggccgttga tccaggcttc gccgaccgtg aaccggtccg
     4981 cgacaaagag cagtccgccg atgtggtcga gctgcgggtg cgtcgcgacc aggtagtcga
     5041 tgtgtctcac gttctggttc cacagcgtgg gggcgacgac caggcgcccg gtatccagct
     5101 cgaagaaccg cttgcccgcg tccacgacca tgacccggcc ggaggggaat cgcaccacgg
     5161 tgctgtcgcc ctggcccacg tccagtatgg tcacctccag ggtggcggac gatttccagt
     5221 tggggatgcc gaccagcgcg ggcacggcga ggatgaccag tgcgccgccc gccaacccgc
     5281 gtttccaggg tttgacccgg cgatccacca gcgacgccat gatcaggtac cacaggccca
     5341 acaccgcgat tgacggcgcc gcgacgtgca cggtcgagag cggccacgcg gcgaagaagt
     5401 ccaccacgcg cagcagcgcg tgcaacgcgg ccgcgttcca ttctgccaag ggcagtgtgg
     5461 gcgagtccgt cagcgccgcg gcgatgccgg acatcagacc gaggggcacc accagcgctc
     5521 cggccagcgg gatgatgatc ggattggcga tcacgcccac ccagttgacc tggaagaagt
     5581 gatacgccac cagcggggcg gtacccacgc ccgcggccac ggaggtgagc gtcagggccg
     5641 acgccctggc gcgaagccgg tcctgccagc gaaccggtcc gggtggttcc ggcgagaact
     5701 cgtggcccga cgaccactcc agggccaatg cgatacacca caccgagagg aacgagagtt
     5761 ggaacgacac gtcggtcaac gcgtgcgggt cccacgcgag caatgcgatc gccgcgaagg
     5821 cgagcagatt caagaggtcg ccgtggcggt ggatgagcac ggcggacaaa tacaccagga
     5881 tcatgagcaa cgcgcgcatg gtggcgactt gggcgccggc gagcagcgcg taaaagacca
     5941 ccgggatcat ggtggccgcg gccgcgattt gcgacggcat gacgcgcatg gtgacgcgca
     6001 gcagcaggcg ctccggcaga cggagcacga tcagacgaac cgcggcgaag acgatcagcg
     6061 cgatgagccc gaggtgcgag ccagaaatcg acaagaggtg cacggtgccc gaggccatga
     6121 accgatcgcg caggtccgag gtcagatagc cggtttctcc ggtcaccatg gccagaaaga
     6181 tcggcgcgac gtcggccggc agcgaggcga cggccgcgac gcggatgcgt tcgcgccact
     6241 catacacgcg atcgaacacc ggcgagtcgc gatgacccgt gcgcgcgatc tcaccgggct
     6301 tgaccgtggc gaccgcgatg acgccctggc gctccagcca gcggccgtag tcgaagagcc
     6361 cggggttttg aaacccgcgc ggcgggcgga attggaccgt gacccgaatc cggtcgccgt
     6421 agcgcaacgc cgcgagcgcg tcgggcggac cggacagttt gatccgccca cggagatcat
     6481 caggcttctt gcccgcgagt cggaggtccg acgcggtgac ggaggtccag tcggtcatgt
     6541 ggcggaccgg ctcgtccaga accgcgtcca ccgtgaccgg ttcgctcgtt agtcggggat
     6601 cgggcgcagg gggcggagtg gcgagggtgt aggcggccgc ggcaaggatc accgcccaat
     6661 gaaccgggcc gaaggacact gcgcggggga gcttccggcg tcccagtccg gttccggcga
     6721 tcacgaccag agccagcgcc gcggcggtga tcggaaaacc gtcggcgagc gcgcccaatg
     6781 cgatgccgac ggccatcgcc agggcgaatg ggatcagcgg gcgtcgcacc cgcgtgtccg
     6841 ccgagatctg gacatcggcg ggcggggagc ccgtgtcagg aggcagtggt cggtcgcttg
     6901 tcagcggtcg tgacagacga agcagagatc ctgcgccggc gcgcggagaa acggcgcgct
     6961 cccgttatcg tgcgggtcgt gacagctgcc gcactgcacc atgccgtccg acgtgcggac
     7021 cgaacgcaac gccgcggcgg ccggatcgga cgcggccagg accgcggagc tcggaccagt
     7081 gggggagatc agattgccgt cgcgcagatc cgggatcgac caataccgat attggttcgc
     7141 ggtcggagtt ttggggacga accggccgtc cggcaggcgc gggtacacca cgttgctggg
     7201 gtggtcgaag gtcttgccgt tgcgcacgag cgatgcgtgg atctcctgtc ccagtacgcc
     7261 gtcgtggcac tccaggcacg cgtcggatcc gttgcccacg gacgccgcgg ccggccgcgt
     7321 ggccgaataa atccggtagg gcgcagcgct tcgttgccac aggggcggag gcggtgcgga
     7381 ctctccttcc ggggcgaggg cctcgtcggg ttgagccggg ctctccgcgg ccggcgcccc
     7441 cgcgccctcg gcgtggcacc cggcgcagac gcgtcggccg atcggaaatt ccgatcgcgc
     7501 aaagacattg tgcgggctgt tgatgaccgc ttcatgcgga tcggccgccg cggccgggtt
     7561 ggcctgcgtc gccgcggcga gcaggagcgc ggcgcacaac cgtttcatgc ggcgcccatg
     7621 ggcgcggacg gcgccccgac cagggttctc agggtctccg cgagttcttc ggcgaccgtg
     7681 gaaatggtct ggaaatcttc gccctcgacc atcacccgga gcaggggttc ggttccggaa
     7741 tagcggatca gcaggcggcc ttgggggccg agccgtcgct cgaagttcga caccagcgcc
     7801 gcgtaaccgg ggaccgtgtt caagtcgcgg cgttcggcgg cccggacgtt caccagcacc
     7861 tgggggcaca ccgccatgca cgaggccagc tcggacagtc gctttccggt ccgttccatg
     7921 gtcgccagga cttggaccgc ggtgatcagg ccgtcgcccg tggtgttgta atcaaggaag
     7981 atgatgtgcc cggactgctc gccgccgaac acgtagccgt ctttgagcat gcgttccaac
     8041 acgtaccggt cgccgaccgc ggtccgcacg agcgaaatcc cggcgtgccg cagcgcgagt
     8101 tccagcccta ggttgctcat caccgtggcc acgaccgtgt ggccggccag gcggcccacg
     8161 cgttcaagct ccaacgcgca taacgcgagg atctggtcgc cgtggacgat tttgccctgg
     8221 tcgtcgcaaa agaccacccg gtccgcgtcc ccgtcgtggg cgatgccgag gtccgcgtgg
     8281 tgcatggcca cggccttttg gagcgcctcc gggtagaggg cgccgcagtg ctggttgatg
     8341 ttggtgccgt tggggcggtc cgcgatcgtg atcacctcgg cgccaagctc cctcagcacc
     8401 atgggcgtga ccttgtaggc ggcgccgttg gccgtgtcca cgacgatctt catgccgtcg
     8461 aactgcatgc ccttgggcaa cgtgttcttc acgaactcga tataccgtcc atccgcgtcg
     8521 tccacgcgaa aggccttgcc gatctcgcgg gcggtggggc gttcgtgctc gatcgcaccg
     8581 gaaaaaatca ggtcctcgat ctcctgttcg acgctgtccg gcagcttcag accatcgtgc
     8641 gaaaagaact tgatcccgtt gtcttcgaac gggttgtggg acgcggagat caccacgccg
     8701 gcgtccgcgc gcaggctctt ggtcaaaaag gcgatggcgg gggtgggcat ggggccgacc
     8761 agcaacacgt cgacgcccat ggaacagacg cccgcggtca acgcgctctc gatcatgtag
     8821 ccggacaagc gggtgtcctt gccgatgacg aagcggtggt ggcccgtgtg gtgcttcttg
     8881 aacaggtagg cggccgcgcg ccccaatttc atcgcggttt cggccgtgac gggctcgatg
     8941 ttggcggtcc cgcggacccc gtcggttcca aacagcctgc gttctacgcc gagcttggac
     9001 acaatggggt gatcttagca ggcgaatccc accttttcaa ccgagcaagg agcctgtcca
     9061 tgagcggtca tctgcggcgt tgtcacgtcg gcttcgcatc ctcacgtacg acccagtacg
     9121 ctgcggtgcg aagcctcgtt ccgccttgca gctgacgc
//
LOCUS       DBNGTT010000348        10899 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_211222, whole genome shotgun sequence.
ACCESSION   DBNGTT010000348 DBNGTT010000000
VERSION     DBNGTT010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_211222"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="AC16BC_07795"
     CDS             complement(<1..143)
                     /locus_tag="AC16BC_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR3241562.1"
                     /translation="MNEASVRDEPRETSSTRPFRSGRIALVGRPNVGKSTLINRLVGE
                     KVA"
     gene            complement(140..559)
                     /locus_tag="AC16BC_07800"
     CDS             complement(140..559)
                     /locus_tag="AC16BC_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR3241563.1"
                     /translation="MSRRYHRGERSDWRSKPVRVYRGGTWSRANRRKTDAERRRRAGR
                     LLTVTVGVPLIVVLAGALLFGERGLWRVAGMADHQRQLKQDIAAVEARNAELQADIAR
                     LRSDPFTIEAIARERLGMGRKGEIVYYFQSPDKTETR"
     gene            complement(556..1863)
                     /gene="eno"
                     /locus_tag="AC16BC_07805"
     CDS             complement(556..1863)
                     /gene="eno"
                     /locus_tag="AC16BC_07805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR3241564.1"
                     /translation="MSEIVQVFGREIMDSRGMPTVEAEVVLDSGATGRAAVPSGASTG
                     EREALELRDRDAKRYRGKGVLKAVRQVNETIGPALVGFEAGEQAVVDGAMIELDGTPS
                     KQKLGANAILAVSLAVLHAAAAEQRVPLYRYIGGLRARRLPVPLMNILNGGAHADNGI
                     DVQEFMIIPRGSGSFGEALRVGAEVFHALKEVLKQAGHTTAVGDEGGFAPRLGSNEEA
                     LTVVVRAIREAGYEPGREVAIALDVASSELYAKGKYAFRAGGKAERTAEQLVDWYEKL
                     LDRFPIVSIEDGMAENDWKGWRLLTERLGSRVQLVGDDLFVTNAELLARGIADGCGNA
                     ILIKLNQIGTVTETLETVELAQKAGYGVIISHRSGETEDTTIADLAVGVNAGQIKTGS
                     LSRTDRVAKYNQLLRLEEDLAEAGRYESGDLYAAAGSRGRTRR"
     gene            complement(1868..2359)
                     /locus_tag="AC16BC_07810"
     CDS             complement(1868..2359)
                     /locus_tag="AC16BC_07810"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIR3241565.1"
                     /translation="MTWRVVGGAAVAALLGVALSAGGRVLEHSPTFCVSCHEMERPGK
                     GWKASGASEHHPDCIMCHSGPGPFGVVEAQTRGLGMLVQHFVESEEKLKGPFKAKVPD
                     RFCTQCHEPVKIEAPHKKFSTVGKSCRDCHKHREDWEFNGELRGSGESAGETDASITW
                     EKD"
     gene            complement(2356..3807)
                     /gene="gatB"
                     /locus_tag="AC16BC_07815"
     CDS             complement(2356..3807)
                     /gene="gatB"
                     /locus_tag="AC16BC_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3241566.1"
                     /translation="MSASAATGTAYETVIGLEVHAQLLTSSKMFCACPTTFGSAPNTQ
                     VCPVCLGMPGVLPVVNRKAVEFAIRFGLAVGAAVSASRFARKNYFYPDLPKGYQISQY
                     ESPICGGGGLDVAFNGGTKRVRLVRAHLEEDAGKNLHDVGDGESHVDLNRAGTPLLEI
                     VSEPDLRSSDEAVGYLKALRELVVYLGICDGNMEEGSFRCDANVSIRPMGSPTLGTRV
                     ELKNINSFRFVKRAIEYEVERQIERVEAGERIAQETRLFDNQRGVTVSMRSKEEAHDY
                     RYFPDPDLVTVAPPEAWIEQIRAAMPELPEAKRARFAEQYGLSAYDATVLTGSRALAE
                     YYEAAVRHGAPAKAAANWIMSELLALLNADGLEIDACRVSAKSLAALFGLIDNNTISG
                     KIAKTVFEEMYRTGADPESVVAAKGLTQVSDAGELAAVIDQVLADSPKELEQYRSGKD
                     KLFGFFVGQVMKRTQGKANPAKVNELLKAKLAG"
     gene            complement(3815..5287)
                     /gene="gatA"
                     /locus_tag="AC16BC_07820"
     CDS             complement(3815..5287)
                     /gene="gatA"
                     /locus_tag="AC16BC_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR3241567.1"
                     /translation="MAEQALHGLTIHEAAAMLAARRISSRELLESVESRIAAVEPEVH
                     AYLSQTRDLARSQADEADRRIRAGSAVTPLTGIPIALKDNMCVDGVKTTCASKILEGF
                     VPPYDATVVARLRAAGAVFVGKTNMDEFAMGSSTENSAFGHTRNPWGRDRIPGGSSGG
                     SAAAVAADECLGALGSDTGGSIRQPAACCGVVGLKPTYGRVSRYGLVAFASSLDQIGP
                     FAKDVEDCALLLNAIAGHDANDSTSAEVPTPDYTADLANGIRGLRVGVPKEYFIPGMD
                     RGVEDAVKTAIRILEREGAVIEDISLPNTEYAVAVYYLLATAEASSNLARYDGVRYGY
                     RAKAPSNLMDLYFRSRAEGFGPEVKRRIMLGTYALSSGYYDAYYVKAQRVRTLIKRDF
                     DRAFERVDVIATPTAPTPAFRFGEKTQDPLQMYLSDIFTISVNLAGVPALSMPCGLSG
                     EGLPIGLQLIGKPFAESTILRAAAAYEGATDWTLRKPAIG"
     gene            complement(5280..6785)
                     /locus_tag="AC16BC_07825"
     CDS             complement(5280..6785)
                     /locus_tag="AC16BC_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR3241568.1"
                     /translation="MRINALNRPEMAFISEIATRFPQAQVYLVGGAVRDAMLGRETKD
                     LDFVVRGVPGEALARALANLGSVNYVGKTFGVYKFVPRGGDPQEPIDVALPRTEHSET
                     LSGAYREFAVQSDPMLPVEEDLGRRDYTVNAMALNVLTDELVDPFGGKGDLCARRLRA
                     VGEADRRFAEDYSRMLRGLRLACQLGFAWDDATWASLVRLMPQINATRGADFVVPRET
                     VGRELVRAFAADPVKALDLWDASGAVTAVMPELLPMKGCPQPLNYHAEGDVWTHTRLA
                     LERLASADFAGEWPERPPDAEVIFAVLLHDVAKPVTITTPEEDGADRIRFNNHDNVGA
                     RMARAICERLKLSQFPRESRWHIDAERLGWLVAHHLLLVHGPIEDMKNSTIEKYFFAD
                     PKAGEQLLMVMWADGSATVPPSGTPDLRNYRAMRARIAKLEELGRARKGLPPPLVDGT
                     RIMTEFGVRPGPTVGKYLALVREAQLAGEVATADDAIAWLKRQIGTMVSDG"
     gene            complement(6874..7224)
                     /gene="panD"
                     /locus_tag="AC16BC_07830"
     CDS             complement(6874..7224)
                     /gene="panD"
                     /locus_tag="AC16BC_07830"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIR3241569.1"
                     /translation="MLRLMLRSKIHRATVTGADLDYEGSLALDQDLIDAAGMLPYEQI
                     MVSNLQNGERFETYIIPGPRGSGMVCLNGPTARKAMIGDQIIIFCYEYYSQEEMASYK
                     PTIVKVDEKNRMKS"
     gene            complement(7394..7681)
                     /gene="gatC"
                     /locus_tag="AC16BC_07835"
     CDS             complement(7394..7681)
                     /gene="gatC"
                     /locus_tag="AC16BC_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR3241570.1"
                     /translation="MDITREQVEYAAKLARLAISEEETAVFAKQLSSILTYVETLNRV
                     DTSQVEPTSHVIPMQNVLREDVVKPPLSQDAALANAPDADAGCFRVPKIIE"
     gene            complement(7681..10032)
                     /locus_tag="AC16BC_07840"
     CDS             complement(7681..10032)
                     /locus_tag="AC16BC_07840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3241571.1"
                     /translation="MNDLLSELNPAQREAVLHTEGPLLILAGAGSGKTRVITYRIAHL
                     IETHRVHPAQILAVTFTNKAAGEMKDRVERLLGPAGRGVWVSTFHSACVRILRTHAER
                     LSYPREFVIYDAGDQAALVRDCARSLGINEDVYKPQAIARRISDLKNALTTPEAFRGT
                     AQTFGMDDAVALTYLLYQDRLAQAGAWDFDDLLMQTVRLFERAPDVLAAYHRQFRYLL
                     IDEYQDTNHAQYRFVRLLAGERANVCVVGDDDQSIYAFRGADVRNILEFEKDFPNAAT
                     VTLEQNYRSTQAILDAASAVVERNPSRKPKRLWTDRLGGEKITVARLPDDETEADYLC
                     RTLREHVRAGKTYRDFAVLYRTNAQSRALEERLRNEAIPYVIVGGLRFYERKEIKDAV
                     AYLRAIVNPADTMSLKRIINVPTRGIGATALERIDAYATQAGVPWAEALRAVAADPER
                     LVAGSRRAIERFLTLMEGLRETAKSAALPALLTAVLERTRYVESLREEFGADAESRIE
                     NIQELFSAVEEFEEQYANQATGEPGPPGEDAVPRLSPLAAFLDRVALVSDTDALTSTD
                     GAVPLMTLHSAKGLEFPVVFLVGVEEGLFPHSRSLNDPDAMEEERRLCYVGITRAKER
                     LYVTYTGLRRLYGSVQFNAPSRFIEEIPEDLKREIGVQEPAATGAGRAWESPGRGVSR
                     TGALPGARAPERAALLRHPEGAERLTGAPSAHGGFNPYNQEPPEAAEGSAFAIGARVR
                     HPVWGAGTIQASEGVGEDAKVVVAFRAAGTKKLAVKFARLEPA"
     gene            complement(10186..>10899)
                     /locus_tag="AC16BC_07845"
     CDS             complement(10186..>10899)
                     /locus_tag="AC16BC_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3241572.1"
                     /translation="CNVVGSTIAREADGVLYTHAGPEIGVASTKAFVCQLAGMVLLAL
                     AVGQAKGRLDAAIRKRLADAMFELPGRVESWLNHSAKAVADVARRFSGRSDFLFLGRG
                     IQYPIALEGALKLKEISYIHAEGYPAGEMKHGPIALIDENLPVVMLAMRDAAYDKSVN
                     GLMEVKAREGQVIAIVTEGDEENAAKADAAISVPALDPLVMPIMAVVPLQLLAYHIAV
                     LRGSDVDQPRNLAKSVTVE"
BASE COUNT         1718 a         3959 c         3292 g         1930 t
ORIGIN      
        1 atcgccacct tctcgcccac gaggcggttg atcagcgtcg acttcccgac gttgggccgc
       61 cccaccagcg cgatccgtcc gctgcgaaac ggacgcgtcg acgaggtttc gcgcggctcg
      121 tcccgaacgg atgcctcgtt caccgcgtct ccgtcttgtc gggcgattga aagtagtaca
      181 cgatttcccc cttgcgcccc atgccgagcc gctcacgcgc gatcgcctcg atcgtaaacg
      241 ggtcggaccg caggcgggcg atatcggcct gcagctccgc gttgcgggct tcgaccgcgg
      301 cgatgtcttg cttgagctgc cgctggtgat ccgccatgcc ggccacccgc cacaatcccc
      361 gctcgccgaa cagcaacgcg ccggccagga ccacgatcag cgggacgccc accgtcaccg
      421 tgaggaggcg ccccgcgcgc cgacgccgtt cggcgtcggt cttcctccgg ttggcccgcg
      481 accaggtccc gccccggtac acgcgaaccg gcttggaccg ccagtcggac cgttcaccgc
      541 gatggtatcg tcggctcacc gtctggtccg tccgcgcgat ccggcggccg cgtacagatc
      601 cccggattcg taccggccgg cctcggccag gtcctcttcg agccgcagca gttggttgta
      661 cttggcgacc cggtcggtgc gcgacaacga tccggtcttg atctgccccg cgttgacccc
      721 caccgccagg tccgcgatgg tggtgtcctc ggtttctccc gagcgatgcg agatgatcac
      781 cccgtagccg gctttctgcg ccagttccac ggtttccagc gtttcggtca cggtgccgat
      841 ttgattgagc ttgatgagga tcgcgttgcc gcagccgtcg gcgatgccgc gcgcgagcaa
      901 ttcggcgttg gtcacgaaca gatcgtcgcc caccagttgc acgcgggagc cgaggcgttc
      961 ggtgagcagc cgccagccct tccaatcgtt ctccgccatg ccgtcctcga tcgagacgat
     1021 cggaaaccgg tcgagcagct tctcgtacca gtccacgagc tgttcggcgg tccgctccgc
     1081 cttcccgcca gcgcgaaacg cgtatttgcc cttggcgtac aactcgctcg acgccacgtc
     1141 gagcgcgatc gccacctcgc ggcccggttc atacccggcc tcgcggatcg cgcgcaccac
     1201 cacggtcaac gcttcctcgt tcgagcccaa ccgcggcgcg aatccgcctt cgtctcccac
     1261 cgcggtcgtg tgcccggcct gtttcaggac ctccttgagc gcgtgaaaca cctccgcgcc
     1321 gactctcagg gcttcgccga acgaacccga gccgcgcggg atgatcatga actcctgcac
     1381 gtcgatgccg ttgtccgcgt gcgcgccgcc gttgaggatg ttcatcaacg gcacgggcag
     1441 cctgcgcgcg cggaggccgc cgatgtagcg atagagcggg acgcgctgtt cggcggccgc
     1501 ggcgtgcagc acggcgagcg acacggccag aatggcgttg gcgcccagtt tctgtttgga
     1561 cggcgtgccg tccagctcga tcatggcccc gtccaccacg gcctgctcgc cggcttcgaa
     1621 tcccaccagg gcggggccga tcgtttcatt gacctggcgc accgctttga gcacgccctt
     1681 gccgcgatac cgcttggcgt cgcgatcgcg cagctccagc gcttcccgtt ccccggtcga
     1741 ggcaccggac ggcaccgcgg cccgcccggt cgcgccgctg tccaggacca cctccgcttc
     1801 cacggtcggc atcccgcgcg aatccatgat ctcccgcccg aacacctgca cgatctcgct
     1861 catctgatca gtccttttcc cacgtgatgg atgcgtccgt ctctccggcg gattccccgg
     1921 aaccccggag ctcgccgttg aactcccaat cctcccggtg cttatggcaa tcccggcacg
     1981 acttgcccac ggtcgaaaac ttcttgtgcg gcgcttcgat cttcacgggt tcgtggcact
     2041 gggtgcagaa ccggtccggc accttggcct tgaacggccc cttgagtttc tcttcgctct
     2101 cgacgaagtg ctggaccagc atgcccaggc cgcgcgtctg cgcctccacg acgccgaacg
     2161 ggcccggacc ggagtgacac atgatgcagt cgggatgatg ctccgaggct ccggacgcct
     2221 tccatccctt tccgggacgc tccatctcgt gacacgagac gcagaacgtc gggctgtgtt
     2281 cgagcacgcg ccctcccgcg gacaatgcca ccccgagcag ggccgccacc gccgcgccgc
     2341 cgaccacgcg ccacgtcatc ccgccagctt ggccttgagc aattcattga ccttcgcggg
     2401 attggccttg ccctgcgtgc gtttcatcac ctggcccacg aagaacccga acagtttgtc
     2461 cttgccggaa cggtactgct cgagttcctt ggggctgtcc gccaggacct ggtcgatgac
     2521 cgcggcgagt tcgccggcgt cgctgacctg ggtcagcccc ttggccgcca ccaccgactc
     2581 cggatccgcg ccggtgcggt acatctcctc gaacacggtt ttggcgatct tcccgctgat
     2641 cgtgttgttg tcgatcaagc cgaagagcgc ggccagcgac ttcgcggaca cgcggcacgc
     2701 gtcgatctcc agcccgtccg cgttgagcag cgcgagcagc tcgctcatga tccagttggc
     2761 cgcggccttg gccggcgcgc cgtgtcggac cgcggcctcg taatactccg cgagcgcgcg
     2821 gctgcccgtg agcacggtgg cgtcgtacgc cgacaggccg tactgctccg cgaaccgcgc
     2881 gcgtttggct tccggcagtt ccggcatggc ggcgcggatc tgttcgatcc aggcttcggg
     2941 cggcgccacc gtgacgaggt cgggatccgg gaaatagcgg taatcgtgcg cctcttcctt
     3001 gctgcgcatg gacaccgtga cgccgcgctg attgtcgaac aggcgcgttt cctgggcgat
     3061 gcgctcgccc gcctcgaccc gctcgatctg gcgctccacc tcgtactcga tcgcgcgctt
     3121 cacgaaccgg aacgagttga tgttcttgag ttccacgcgc gtgccgaggg tggggctgcc
     3181 catcggccgg atcgacacgt tggcgtcgca ccgaaagctc ccctcttcca tgttgccgtc
     3241 gcaaatcccc aagtacacga ccagttcgcg cagggccttg agatacccaa ccgcttcgtc
     3301 ggacgagcgc aggtccggct cgctgacgat ctccagcagc ggcgtgcccg cccggttcaa
     3361 gtccacgtgg ctctcgccgt cgcccacgtc gtgcaggttc ttgcccgcgt cctcctccag
     3421 gtgcgcgcgg accagccgca cgcgcttggt gcctccgttg aacgccacgt cgagcccgcc
     3481 cccgccgcag atcggcgact cgtattgcga aatctgatac cccttgggca gatccgggta
     3541 gaagtaattt ttccgcgcga agcggctggc cgacaccgcg gcgccgacgg ccaggccgaa
     3601 gcggatcgcg aactccaccg ccttccggtt gaccaccggc aacacccccg gcatccccag
     3661 acagaccggg cacacctggg tgttcggcgc cgacccgaac gtggtggggc acgcgcagaa
     3721 catcttcgaa ctggtcagca actgggcgtg cacctccagg ccgatcacgg tttcgtaggc
     3781 ggttcctgtc gcggctgatg cgctcatggt cgtgtcatcc gatcgccggt tttcgcaatg
     3841 tccagtccgt cgcgccctcg tacgccgcgg ccgcgcgcag gatcgtggat tccgcgaacg
     3901 gtttgccgat caattgcagc ccgatcggca agccctcgcc cgacagcccg cagggcatgg
     3961 acagggccgg cacgcccgcc agattcaccg agatcgtgaa aatgtcggac agatacattt
     4021 gcagcgggtc ctgcgttttt tccccgaaac gaaacgcggg ggtgggcgcg gtgggggtgg
     4081 cgatcacgtc cacgcgttcg aacgcgcggt cgaagtcgcg tttgatcaac gtgcgcacgc
     4141 gctgggcctt gacgtagtac gcgtcgtagt agccggagga caaggcgtag gtgccgagca
     4201 tgatccggcg tttgacctcg ggaccgaacc cttcggcccg gctgcggaaa tacaggtcca
     4261 tgaggttcga gggcgctttc gcgcggtatc cgtatctaac gccgtcgtac cgcgccaggt
     4321 tggagctcgc ctcggccgtg gccagcaggt aatacaccgc taccgcgtat tccgtgttcg
     4381 ggagcgagat gtcctcgatc accgcgccct cgcgttcgag gattcggatc gcggttttca
     4441 ccgcgtcttc gaccccacga tccatgccgg gaatgaagta ctccttgggc acgcccacgc
     4501 gcaggccccg aatcccgttc gccaggtccg cggtgtaatc cggggtcggc acctcggccg
     4561 acgtggagtc gttcgcgtcg tgacccgcga tggcgttgag caacaacgcg cagtcctcca
     4621 cgtctttggc gaacggcccg atctggtcca gcgaggacgc gaacgccacc aacccgtacc
     4681 gcgagacccg gccgtacgtg ggcttgagcc ccaccacgcc gcaacaggcc gcgggctggc
     4741 ggatggaccc gcccgtgtcc gatcccaacg ctcccaaaca ctcgtccgcc gccaccgccg
     4801 cggccgatcc gccgctcgac ccgccgggga tccggtcgcg gccccacggg ttgcgggtgt
     4861 gcccgaacgc ggagttctcg gtggacgagc ccatcgcgaa ttcgtccatg ttggtcttgc
     4921 ccacgaacac cgcgcccgcg gcgcgcaatc gggccaccac cgtggcgtcg tagggcggga
     4981 cgaagccttc gaggatcttg gacgcgcacg tggtcttcac gccgtccacg cacatgttgt
     5041 ctttgagcgc gatggggatg ccggtcaacg gcgtcaccgc ggagccggcg cgaatccgcc
     5101 ggtcggcctc gtccgcctgg ctgcgcgcca gatcgcgcgt ttgcgacaga tacgcgtgga
     5161 cctcgggctc caccgccgcg atccgtgact ccacgctctc caagagctcg cggctgctga
     5221 tgcggcgggc cgccagcatc gcggccgctt cgtgaatggt caatccgtgt aaggcttgct
     5281 cagccatccg acaccattgt tccgatctgc cgcttgagcc acgcgatcgc gtcgtccgcg
     5341 gtcgcgacct cgccggccaa ctgcgcttcc cgaaccaacg ccagatactt gccgaccgtc
     5401 ggccccggcc ggacgccgaa ctccgtcatg atgcgcgtgc cgtccacgag cggcggcggc
     5461 aggcctttgc gcgcgcggcc cagttcctcc agcttcgcga tccgggcgcg catggcgcga
     5521 tagttgcgca gatccggtgt ccccgacggc ggaaccgtgg ccgacccgtc cgcccacatc
     5581 accatcaaca gttgctcgcc cgccttcggg tccgcgaaaa agtatttctc gatcgtcgaa
     5641 ttcttcatgt cctcgatcgg gccgtgcacc agcaacagat ggtgcgccac caaccacccc
     5701 aaccgctcgg cgtcgatatg ccatcgagac tcgcgcggga actgggacag cttcaatcgc
     5761 tcgcagatcg cgcgcgccat tctggcgccc acgttgtcgt ggttgttgaa ccggatccgg
     5821 tccgcgccgt cttcctccgg cgtggtgatc gtgaccggtt tggccacgtc gtgcagcagg
     5881 accgcgaaga tcacctccgc gtccggcggc cgctccggcc actccccggc gaagtccgcc
     5941 gacgccaacc gctccagcgc caaccgcgtg tgggtccaga cgtctccctc cgcgtgatag
     6001 ttgaggggct gcggacagcc cttcatgggg agcaactccg gcatcaccgc ggtcacggcg
     6061 ccgctcgcgt cccagagatc caacgccttg accgggtcgg cggcgaacgc gcgcaccagc
     6121 tcccgcccca cggtctcgcg cggcaccacg aagtcggcgc cccgcgtcgc gttgatctgc
     6181 ggcatcaaac gcaccagcga cgcccacgtg gcgtcgtccc atgcgaatcc gagctggcag
     6241 gccaatcgca atccgcgcag catccgcgaa taatcctccg cgaatctccg atccgcctcc
     6301 cccaccgcgc gcaaccgtcg ggcgcacaga tcgcccttgc cgccgaacgg atcgacgagt
     6361 tcgtcggtca gcacgttgag cgccatcgcg ttcacggtgt agtcgcgccg ccccagatcc
     6421 tcctcgaccg gcaacatcgg gtcggactgc accgcgaact cgcggtacgc ccccgacaat
     6481 gtttccgaat gttcggtgcg cggcagcgcc acgtcgatgg gctcctgcgg atccccgccg
     6541 cgtggaacga acttatacac gccgaacgtc ttgcccacgt aattgacgga cccgaggttg
     6601 gccaacgcgc gagccagcgc ctcgcccggc accccgcgca ccacgaagtc caaatccttg
     6661 gtttcgcgcc ccaacatcgc gtcccgcacc gccccgccca cgaggtacac ctgggcctgc
     6721 ggaaatcgcg tagcgatttc cgagataaag gccatctctg gccgattcaa ggcgttgatg
     6781 cgcattgcat tcccattgat gaaacgacga ccgaccggtc atccctccga tgactgatgt
     6841 cccgttccgg taggggcacg gcatgccgtg cccctacgat ttcattcgat tcttttcgtc
     6901 gaccttcaca atggtcggct tgtagctcgc catctcctcc tgcgagtagt actcgtaaca
     6961 aaaaatgatg atctgatcgc cgatcatcgc cttgcgcgcg gtcggtccgt tcaaacacac
     7021 catgccggac ccgcgcggac cggggatgat gtacgtctcg aaccgttcgc cgttctggag
     7081 gttggacacc atgatctgct cgtacggcaa catgccggcc gcgtcgatca gatcctggtc
     7141 caacgccaga ctgccttcgt aatccaagtc cgcgcccgtc accgtggcgc gatgaatttt
     7201 cgaccgcagc atcaaccgca acatacgtct cctcgtccag agaatcccga tcccatcatg
     7261 tcgcccgcgg agcagatttc ccctggctcc cggctgacaa agcaaatccc cccttgcccc
     7321 cctttgaaaa aggggggatg gggggattta aaacgtgtac cgcaaaaccc ggcattcatg
     7381 cgcgacaccg aggtcattcg atgatctttg gcacgcggaa acaccccgcg tccgcatccg
     7441 gcgcattcgc caacgccgcg tcctgcgaca acggcggctt gaccacgtcc tcgcgcagca
     7501 cgttctgcat cgggatcacg tgcgaggtcg gctccacctg gctcgtgtcg acccggttga
     7561 gcgtctcgac gtaggtcaag atcgacgaca actgtttggc gaacacggcc gtctcctcct
     7621 cgctgatcgc caatcgcgcc agcttggccg cgtattcgac ctgttcacgc gtgatgtcca
     7681 ttacgccggc tccaatctcg cgaatttgac cgccaatttc ttggtgcccg cggcgcgaaa
     7741 cgccaccacc accttggcgt cttcaccaac cccttccgag gcctggatcg ttcccgcgcc
     7801 ccacaccgga tggcggacgc gcgcgccgat cgcgaacgcc gagccctccg ccgcctccgg
     7861 aggttcctga ttatacgggt tgaacccgcc gtgcgccgac ggcgcacctg ttaatcgctc
     7921 tgctccttcc gggtggcgaa gcaaggcggc gcgctccggc gcgcgggccc cgggcaacgc
     7981 ccccgtgcgg gacacgccgc ggcccggcga ctcccacgcg cgccccgcgc cggtcgccgc
     8041 cggttcctgg accccgatct cgcgcttcaa gtcctcggga atctcttcga tgaaccgcga
     8101 gggcgcgttg aactggaccg agccgtagag ccgccgcaat ccggtgtagg tcacgtacag
     8161 ccgctccttg gctcgcgtga tccccacgta acacaatcgc cgttcctctt ccatggcgtc
     8221 ggggtcgttg agcgagcggg aatgggggaa gagtccttcc tccaccccga ccaaaaacac
     8281 caccggaaac tccagcccct tggccgagtg cagcgtcatc aacggcaccg cgccgtcggt
     8341 cgaggtcaac gcgtcggtgt cggacaccag ggccacgcgg tccagaaacg cggccagcgg
     8401 cgacagccgc gggaccgcgt cctcgccggg cgggccgggt tcgcccgttg cctggttcgc
     8461 gtattgctcc tcgaactctt ccaccgcgga gaacaattcc tggatgttct cgatgcgcga
     8521 ctccgcgtcc gcgccgaact cctcgcgcag cgattccacg tagcgcgtgc gctccaacac
     8581 cgcggtgagc agcgcgggca acgcggccga cttcgcggtt tcgcgcaggc cctccatcaa
     8641 ggtcaggaac cgttcgatcg cccgccgcga gcccgcgacc aggcgctccg ggtccgcggc
     8701 caccgcgcgc agcgcctcgg cccagggcac tccggcctgc gtcgcgtacg cgtcgatccg
     8761 ctccagggcc gtggccccga tcccgcgggt cggcacgttg atgatccgct tgaggctcat
     8821 ggtgtccgcg gggttcacga tcgcgcgcag gtacgccacc gcgtccttga tctccttgcg
     8881 ctcgtagaac cgcagcccgc ccacgatcac gtagggaatc gcctcgttgc gcaaccgctc
     8941 ttccagcgcg cgcgactgcg cgttggtgcg gtagagcacg gcgaagtcgc gatacgtctt
     9001 gcccgcgcgc acgtgctcgc gcaacgtgcg gcacaaataa tcggcttccg tctcgtcgtc
     9061 gggcagccgc gccaccgtga tcttctcccc gccaagccgg tcggtccaca gccgtttggg
     9121 cttgcgcgag gggttgcgct cgaccacggc cgacgccgcg tccaggatcg cctgggtgga
     9181 gcggtaattc tgctccaatg tgacggtcgc ggcgttcggg aaatccttct cgaactccag
     9241 gatgttgcgc acgtccgcgc cgcggaacgc gtagatgctc tgatcgtcgt cgcccactac
     9301 gcacacgttg gcgcgctcgc cggccagcaa tctgacgaaa cggtactgcg cgtgattggt
     9361 gtcctggtac tcgtcgatca gcaaataccg gaactgccgg tggtacgcgg ccaacacgtc
     9421 gggcgcgcgc tcgaagagcc ggaccgtctg catcagcaga tcgtcgaaat cccacgcgcc
     9481 cgcctgcgcc agccggtcct gatacaacag gtacgtaagg gccacggcgt cgtccatccc
     9541 gaacgtctgc gcggtcccgc ggaacgcttc cggcgtggtc agcgcgttct tcagatcgga
     9601 aatacgccgc gcgatcgcct gcggtttgta cacgtcttcg ttgatgccca ggctgcgcgc
     9661 gcagtcacgg accagggcgg cctgatcgcc cgcgtcgtag atcacgaact cgcgcgggta
     9721 gctcaaccgt tccgcgtgcg tccgcaggat ccgcacgcag gccgagtgaa acgtgctcac
     9781 ccacaccccg cggcccgccg ggcccagcag ccgctccacg cggtccttca tttcccccgc
     9841 ggccttgttg gtgaacgtga ccgccaggat ctgcgcgggg tgcacgcggt gggtttcgat
     9901 cagatgcgcg atccgatacg tgatcacgcg ggtcttgccg ctcccggccc cggccaggat
     9961 caacaacgga ccctcggtgt gcagcaccgc ctcgcgttgg gcgggattca attccgacaa
    10021 cagatcgttc atcgctcccc gatcaattca cggtgaagac ggaaccggag aaacggcgaa
    10081 tcggagaatc ggcgcagaaa aatccccggt tctgctcttc gcccactccc cgtttctccg
    10141 gttcaccgat tccccggttc gcttgctgtc tcaacatgcc ccgattcact ccaccgtcac
    10201 gctcttggcc agatttctgg gttgatccac gtcgctgccg cgcaggaccg cgatgtgata
    10261 cgcgagcaac tgcaacggca ccacggccat gatcggcatc accaacgggt cgagcgcggg
    10321 cacgctgatc gcggcgtcgg ccttggccgc gttctcctcg tcgccctcgg tcacgatcgc
    10381 gatgacctgg ccttcgcgcg ccttgacctc catcagcccg ttgacgctct tgtcgtaggc
    10441 cgcgtcgcgc atggcgagca tgaccaccgg caagttctcg tcgatgagcg cgatgggccc
    10501 gtgtttcatc tcaccggccg ggtacccctc ggcgtggatg tacgaaatct ccttgagttt
    10561 gagcgcgccc tcgagcgcga tcgggtactg aatcccccgt cccaagaaca ggaaatcgct
    10621 gcgtccggaa aaccgacgcg ccacgtcggc cacggccttg gcggagtggt tcagccacga
    10681 ctccacccgc cccggcagct caaacatcgc gtcggccaat cgcttgcgga tcgcggcgtc
    10741 gagccgcccc ttggcttgcc cgacggccag cgcgagcaag accatccccg ccaactggca
    10801 cacgaacgcc ttggtcgagg ccaccccgat ctcgggcccg gcgtgcgtgt acaacacgcc
    10861 gtccgcttcc cgcgcgatgg tgctgcccac tacgttgca
//
LOCUS       DBNGTT010000349         2741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2128329, whole genome shotgun sequence.
ACCESSION   DBNGTT010000349 DBNGTT010000000
VERSION     DBNGTT010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2128329"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..2260
                     /gene="glgX"
                     /locus_tag="AC16BC_07850"
     CDS             110..2260
                     /gene="glgX"
                     /locus_tag="AC16BC_07850"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR3241573.1"
                     /translation="MRTMPGSPYPLGATWDGKGVNFALFSEHAERVELCVFESEGAKH
                     ESHRVPLTEQTNRIWHAYLPDARPGMLYGYRVYGRYDPAAGHRFNPAKVLVDPYAKVI
                     ARKPHWDDSLFGYRFGHAATDLAIDRRDTAAFAPLARVVDPAFTWGDDRRPQTPWHDT
                     IIYETHVKGLTARHPEVEPRLRGTYAGLASDPVIRYLKDLGVTAVELMPVHHHVDDRH
                     LVEHGLVNYWGYNTLSFFAPDLRYHSGVMDPVHEFKTMVRTLHEAGIEVILDVVYNHT
                     AEGNHLGPTLSLRGIDNAAYYRLSPKDPRYYVDYTGCGNTLNMQHPQVLQLIMDSLRY
                     WITDMHVDGFRFDLASTLARELRDVDRLGAFFDIIHQDPVISQVKLIAEPWDLGEGGY
                     QVGNFPVLWTEWNGKYRDTVRRFWKGAGGQVAELATRLAGSSVLYEQTGRRPHASINF
                     VTCHDGYTLADLVSYEAKHNEANGEDNRDGCNHNDSWHCGVEGPTEDAEILRLRERQR
                     KNFLATLMLSQGVPMLCAGDELGRSQRGNNNAYCQDNELTWHNWDLDPDQRKFLEFLR
                     DLIRFRRQHPVLRRRTFFQGRRIRGSEIKDIAWFSATGREMTDEEWTADHVRSMGMRL
                     AGDAIEEVDDLGRRIAGESLLVLLNAGTAAVPFVLPAHRAGTRWIAVFDTARNGAPRV
                     MNHLERGGRPYDLQPHSMAVFRLAVAGDRRTERS"
     gene            complement(2530..>2741)
                     /locus_tag="AC16BC_07855"
     CDS             complement(2530..>2741)
                     /locus_tag="AC16BC_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR3241574.1"
                     /translation="GWAIGDASDAANGGDAKALYAKLDDVVLPLLTGPRAAWIAVMKG
                     AIGKNASYFNSHRMMRRYAIEAYLR"
BASE COUNT          510 a          921 c          883 g          427 t
ORIGIN      
        1 atgctatcgg aaactgcggc cgtattcaaa ggcccgcgcg tcgggtgacg ccgcgaccca
       61 atcgtcgagc cggattgaca tctcgcgggc gcgcttctat aaaacactca tgcgcacgat
      121 gccgggatcg ccctatccgc tcggggccac gtgggacggg aaaggcgtca acttcgccct
      181 gttctccgaa cacgcggaac gggtggagtt gtgcgtgttc gagtccgagg gcgcgaaaca
      241 cgagtcgcac cgcgtcccct tgaccgagca gaccaaccgg atctggcacg cgtacctccc
      301 ggacgcccgt cccggcatgc tctacggcta tcgggtgtac ggccggtacg accccgcggc
      361 cggccatcgc ttcaaccccg ccaaggtgtt ggtggatccc tacgccaagg tgatcgcgcg
      421 caagccgcac tgggacgatt ccctgttcgg gtaccgattc ggacacgccg cgacggatct
      481 ggcgatcgac cggcgggaca ccgccgcgtt cgcgcccttg gcccgggtgg ttgatcccgc
      541 gttcacgtgg ggcgacgatc gccggcccca gacgccctgg cacgacacca tcatctacga
      601 gacgcacgtg aaaggcctca ccgcccggca tccggaggtc gaaccgcgcc tgcgcggcac
      661 ctacgcgggc ctggcctcgg accccgtgat ccgctacctc aaggacctcg gcgtcaccgc
      721 ggtggagctg atgccggttc accaccacgt ggacgaccgc cacctggtgg aacacggcct
      781 ggtcaattac tggggataca acacgctctc gtttttcgcc ccggacctcc ggtatcactc
      841 gggcgtcatg gacccggtgc acgagttcaa gaccatggtc cgcaccctgc acgaggccgg
      901 gatcgaagtg attctggacg tggtgtacaa ccacaccgcg gagggcaacc acctgggccc
      961 gaccctgtcg ctgcgtggca tcgacaacgc ggcgtattat cgcctctcgc cgaaagatcc
     1021 gcgctactac gtggactaca ccggctgcgg caacactctg aacatgcagc acccccaggt
     1081 gctgcagttg atcatggaca gcctgcgcta ctggatcacc gacatgcacg tggacgggtt
     1141 ccggttcgat ctcgccagca cgctcgcccg ggaattgcgc gacgtggacc ggttgggcgc
     1201 gtttttcgac atcatccatc aggacccggt catctcccag gtcaaattga tcgcggagcc
     1261 ctgggacctg ggggaaggcg gctaccaggt gggcaacttc ccggtgttgt ggaccgagtg
     1321 gaacggcaag taccgcgaca cggtccgccg gttctggaag ggcgcgggcg gacaggtcgc
     1381 ggagctcgcc acccgcctgg cgggcagcag cgtcctgtac gagcagaccg gacgccgccc
     1441 gcacgcgagc atcaacttcg tcacctgcca cgacggctac acgctcgcgg acctcgtcag
     1501 ctacgaggcc aaacacaacg aggccaacgg cgaagacaac cgcgacgggt gcaaccacaa
     1561 cgacagttgg cactgcgggg tggagggccc caccgaggac gcggagatcc tccggttacg
     1621 cgagcgccag cgaaagaact tcctggcgac cctgatgctc tcccagggcg tgcccatgtt
     1681 gtgcgcgggc gacgaactcg gccggtcgca acgcggcaac aacaacgcct attgccagga
     1741 caatgaactg acgtggcata actgggacct tgatccggac cagcgcaagt tcctcgagtt
     1801 tctgcgcgac ctcatccgct ttcgcagaca gcacccggtg ctccgacgcc ggaccttttt
     1861 ccagggccgc cggatcaggg ggtcggagat caaggacatc gcctggtttt cggccacggg
     1921 ccgcgagatg accgacgagg agtggaccgc ggatcacgtg cggtccatgg gcatgcggct
     1981 cgcgggcgac gccatcgagg aagttgatga cctgggtcgg cgcatcgcgg gcgagagcct
     2041 gctcgtgttg ttgaacgccg ggaccgcggc cgtgccgttc gtgttgcccg cccaccgcgc
     2101 gggcacgcgc tggatcgcgg tattcgacac cgcgcgcaac ggcgcgccgc gcgtcatgaa
     2161 ccacctggag cgcggcggac gcccctacga tttgcagccc cactccatgg ccgtcttccg
     2221 attggccgtg gccggcgacc gtagaaccga aaggtcctga ccagccacaa tcacccccat
     2281 catcgcgtcg cgtgcaccgt agatcttcct ggcaggtaca gaacgtagaa gataacccgc
     2341 aggctgctca aaaagctccg gatgcaaggc cgcagggagg aggaaaccgg agcgtactga
     2401 gtcgtacgtg agggcgagtg gcgcgacgaa gaaggagcgc catgccgcga gcgccgccga
     2461 gtcggtgagg cggccgggcg cgagaagccc agcgacaacg ccgcagatga cccgtcatgg
     2521 acaggctccc taccggaggt aggcttcgat cgcgtaccgc cgcatcatcc ggtgactatt
     2581 aaagtaggag gcgttcttac cgatcgcacc cttcatcacc gcgatccagg cggcgcgcgg
     2641 tccggtgagg agcggcagga cgacatcgtc gagtttggcg tacaacgcct tggcgtctcc
     2701 cccgttggcg gcatccgacg catcgccgat ggcccagccg g
//
LOCUS       DBNGTT010000350         2600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1872100, whole genome shotgun sequence.
ACCESSION   DBNGTT010000350 DBNGTT010000000
VERSION     DBNGTT010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1872100"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="AC16BC_07860"
     CDS             <1..442
                     /locus_tag="AC16BC_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241575.1"
                     /translation="LKEIDDGRLNLMEVANQVRQVNIEAQVYRQVHRHLKRFLKQFIK
                     EPQSEALTHLRDQLSQELVNKGLITTPIPEATFRKVIRDLHREAVYFFEVLPTLVASP
                     AGSDGRRDAYFSSSGVDRYDLEELEREYFHQHRLDEALLQALQR"
     gene            480..1877
                     /locus_tag="AC16BC_07865"
     CDS             480..1877
                     /locus_tag="AC16BC_07865"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR3241576.1"
                     /translation="MTVRSAPRLVSSPLMTLAALSLIAALGVVGSPSVSPAKETGGLK
                     ALTELQDVFVDLAEHVKPSVVNISTGTATPPKSGERFRQRPESPSSGSGVIIDKNGYI
                     VTNNHVVGDAEEVQVRLLDKRKFTGKVVGRDPDTDLAVVKIESKTPLPTVPIGDSTQV
                     KVGQWVVAVGNPFGLEHTLTVGVVSALKRENVNLSRYEDFIQTDASINPGNSGGPLFN
                     IRGEVIGINTAIINFAQGIGFAIPSKMVQDIYPQLIAKGRVSRGWLGVGIQTVTEELA
                     TQFNLDKEQGVLVNEVFDGEPAQKAGVQPGDIILKVDGTAVDTPTSLAKVVAGLTPGK
                     PAKLKILRNGEQLDLPVLLSERKDESETPQAVPVKTPESILGLAIENITKETADQMKL
                     KDTAGVLVTKVDEDSLAGRGGIKEGDVIKEINRHAIADSEDFRKASEALKPDEGVLVR
                     IIREQRGLFLVLNDK"
     gene            2142..>2600
                     /locus_tag="AC16BC_07870"
     CDS             2142..>2600
                     /locus_tag="AC16BC_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3241577.1"
                     /translation="MREWVAEAPLIVTEADGVRVRDENGRWYLDGVSSLWVNVLGHRR
                     QEIDQAVIEQLGRVAHSTLLGCSSPPAILLAERLVALAPPGLTKVFYSDNGPTAVEVA
                     LKMALQYWQQVSPPRPDKTKILAFSLAYHGDTVGAVSVGGIDVFHARFGPY"
BASE COUNT          548 a          853 c          792 g          407 t
ORIGIN      
        1 actcaaggaa atcgacgatg gacggctgaa tttgatggaa gtcgccaatc aagtccgcca
       61 ggtcaacatc gaggcgcagg tgtatcgcca agtccatcga cacctcaagc ggttcctcaa
      121 acagttcatc aaggaaccgc aaagcgaggc cttgacccac ttgcgggacc aattgtccca
      181 ggagctggtc aacaaaggac tcattaccac gccgatccca gaggccacgt tccggaaagt
      241 gatccgggac ctccaccgcg aggcggtgta ctttttcgag gtgctgccga ccctcgtcgc
      301 atcccccgcc ggctcagacg gacgccgcga cgcctacttc tcctccagcg gcgtcgatcg
      361 gtacgacctc gaggaattgg aacgtgaata ttttcatcag caccgactcg atgaagcgct
      421 gctccaagcg ttgcagcgct gaacagatca ccgtcctctt gcagacaagg agtcgcacca
      481 tgaccgttcg atccgcacca cgtctcgtgt cctcccccct catgaccctc gcggcgttga
      541 gcctgatcgc cgccctcggc gtcgtgggct cgccgtcggt cagcccggcc aaggagaccg
      601 gcggactcaa ggccttgacc gaattgcagg acgtgttcgt cgacctggcc gaacacgtca
      661 aaccctcggt ggtgaacatt tccaccggca ccgcgacccc gccgaaaagc ggcgagcgtt
      721 tccgccagcg tccggagtcg cccagcagcg ggtccggcgt gatcatcgac aagaacggct
      781 acatcgtcac caacaatcac gtggtcggcg acgccgaaga agtccaggtc cgcctgttgg
      841 acaagcggaa attcaccggg aaagtcgtcg gccgtgaccc cgacaccgac ctcgcggtgg
      901 tcaagatcga atcgaagacc ccgctcccca cggtgccgat cggcgattcc acccaggtga
      961 aggtgggaca gtgggtggtg gcggtcggga accccttcgg gctggagcac acattgaccg
     1021 tcggggtcgt cagcgccctc aaacgggaga acgtgaacct gtcgcggtac gaggatttca
     1081 tccagaccga cgcgtcgatc aacccgggca acagcggcgg gccgctgttc aacatccgcg
     1141 gggaagtgat cgggatcaac accgcgatca tcaacttcgc gcagggcatc ggcttcgcga
     1201 tcccctcgaa gatggtgcag gacatttacc cgcaactgat cgccaagggc cgggtcagcc
     1261 gcggctggct gggcgtgggg atccagaccg tcacggaaga actcgccacg cagtttaatc
     1321 tggacaagga acaaggcgtg ctggtcaacg aggtcttcga cggggaaccc gcgcaaaagg
     1381 ccggggtcca gcccggggac atcatcttga aggtcgacgg aaccgcggtg gacacgccca
     1441 cgagcctggc caaggtcgtg gccgggctga cgcccggcaa gcccgccaag ctcaaaatcc
     1501 tcagaaacgg cgaacaactc gatctgcccg tgctcttgag cgaacgcaag gacgaatccg
     1561 aaacgccgca ggccgtcccg gtcaagacac cggagtccat tctggggctg gcgatcgaaa
     1621 acatcaccaa ggaaaccgcc gaccagatga aactcaagga caccgccggg gtgctggtca
     1681 ccaaggtcga cgaggacagc ctggccggcc gcggcggcat caaagagggc gacgtgatca
     1741 aggagatcaa ccgccacgcg atcgcggact cggaggattt ccgcaaggcc agcgaagcct
     1801 tgaagcccga tgagggcgtc ctggtccgca tcatccgcga gcaacgggga ctgttcctcg
     1861 tgctcaatga caagtagtcg gacgacgcat cacatcagcg caaggacgat tcactgatgc
     1921 cggtcagtga ccggcacgat catccggccg ctcaaaacgc tccagatgcg aggccgcagg
     1981 gagcgcgaag cccggagcgt acggagtccg tacgcgagaa tgcgccttcg gcatcgcgca
     2041 gcaatcttcg cgcgaccgag cacgaagcag acggacgttt tcagcagctg gcccacctcg
     2101 ccgccctcga ccacgcctat ctgtggcacc ccttcaccca aatgagggaa tgggtcgccg
     2161 aagcgccgct catcgtgacc gaagcggacg gcgtgcgcgt gcgggatgag aacggccgtt
     2221 ggtatctgga cggggtgtcg tcattgtggg tgaacgtcct tggccaccgg cgccaggaaa
     2281 tcgaccaagc cgtcatcgag caactcgggc gcgtcgcgca ctccaccttg ctcggctgct
     2341 catccccgcc cgcgatcctg ttggccgagc ggctggtcgc cctcgcgccc cctggcctga
     2401 ccaaagtctt ttactcggac aacggcccca ccgcggtcga agtcgcgctc aaaatggcgt
     2461 tgcagtactg gcaacaagtc tcgccgcctc ggcccgacaa gaccaagatc ctcgcgttct
     2521 cgttggccta ccacggcgac accgtgggcg cggtcagcgt gggcgggatc gacgtgttcc
     2581 acgcgcggtt cggcccgtac
//
LOCUS       DBNGTT010000351         9482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_467117, whole genome shotgun sequence.
ACCESSION   DBNGTT010000351 DBNGTT010000000
VERSION     DBNGTT010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9482
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_467117"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1264
                     /gene="ald"
                     /locus_tag="AC16BC_07875"
     CDS             161..1264
                     /gene="ald"
                     /locus_tag="AC16BC_07875"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR3241578.1"
                     /translation="MKVGVPREVMEHEYRVGMTPAGVRAVTRLGAQVFVERGAGEGSG
                     FPDRAYEEAGAMLVASTEAVYKMGELIVKVKEPLPKEYPWIQKGQTLFTFLHLAADRR
                     VTEALLDLGVVGIAYETVTLEDGTRPLLRPMSDIAGRLAVQVGASYLMRTNGGSGVLL
                     SGVPGVERGRVTILGGGVVGTSAAKIAVGLGARVTVLERDPNRLHVVDDLMGGRITTL
                     IASADTIHQAVVQADLVVGAVLVSGARTPHLVTGQTVAQMRKGSVIVDVSIDQGGCVE
                     TARKTTQADPVYTAHGVIHYGVANLPGTVPRTSTQALTAVTLPYVMQLVERGVEGALK
                     ADPALGAGLNLYHGKVVHEGVAEAHGMKLESWA"
     gene            1531..1604
                     /locus_tag="AC16BC_07880"
     tRNA            1531..1604
                     /locus_tag="AC16BC_07880"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1565..1567,aa:Pro,seq:cgg)
     gene            1575..1904
                     /locus_tag="AC16BC_07885"
     CDS             1575..1904
                     /locus_tag="AC16BC_07885"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3241579.1"
                     /translation="MSEVQILSPRFFRGVMAEAKVYQATVTVSGQVQGVGYRNFAAAE
                     ANRLGLRGHVKNLRDGRVEVRLEGAREVIDLVIGRLRIGPTAACVESVDVRWQEGSSG
                     ARGFTVA"
     gene            1897..2958
                     /locus_tag="AC16BC_07890"
     CDS             1897..2958
                     /locus_tag="AC16BC_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3241580.1"
                     /translation="MPNPKSRKAVLPKRPRKPKDVTLDDEPVEPSESASDEGFPLKTE
                     VEDEGHGEANGDEDVEFEARETVIEPEPESEPGGAMDAVKSYLKEIRRSTLLTFADEQ
                     RLAKLIEKGDAKAREQMIESNLRLVVSIGKRYINRGLPFSDILEEGNIGLIKAVEKFN
                     YRRGFKFSTYASWWIRQSIERAIINQSKLIRLPVHVVEKVNHYLQAVDALMQEYDRDP
                     TAKELSRRLRIPVEEVVHLQTLIRKTYSLESPIGSAQETTLKDIIEDTSQVSPAATTE
                     GIRRREDIIEWMKVLKPNEQKVLIMRFGLDGDEPHTLEEIGKVFGLTRERVRQIEAGA
                     LAKLRGVIGKKSITAEQII"
     gene            2978..3490
                     /locus_tag="AC16BC_07895"
     CDS             2978..3490
                     /locus_tag="AC16BC_07895"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012163376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR3241581.1"
                     /translation="MDLKAKIREVPNFPKPGILFYDVTTLLRDADAFGRVIHRVAEFY
                     RGEGITKIVGIESRGFILGAPVAVQLGAGFVPVRKPGKLPAEVYEKTYDLEYGSSSLA
                     IHKDAIDRGERVLIVDDLLATGGTMAATVHLVNELGGVVVGVAVLIELVELRGRDKLR
                     DQHVLSILSY"
     gene            3504..5354
                     /locus_tag="AC16BC_07900"
     CDS             3504..5354
                     /locus_tag="AC16BC_07900"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HIR3241582.1"
                     /translation="MGRHRPIPVLVAWGLCVAAMTWPTSDALAKEINVPADAASIQAA
                     INLSNQSTDASDTIHIATGTYIENLTISKPTAVAAKSLTIRANDGAKVVLQAAAPGSP
                     LVQIGAGAVVTVERLILVTSGGRGVVMDAATASLRNLVITQASTAIECSVATSGNTVE
                     QVTFSQVANGINCVSSTLTIRNNIFANLSGIPIAPFSPGSGATLPRFNLFFQSGTSGE
                     RGTNEVVARAGDDLDPQFVDVANNDFHIQEGSAAQGTGEGGVDVGAYGGPSSHTIPFP
                     PSKPSISNCTTTPPLACKVTWAKNLDYLVTGYVVLSHATTTPTAPSSSDYGSPVPVDN
                     ATATCEVSNDTTCETTLSSLPNFTTTPASPVHLRASVGSGKVQLAWDRVSNSNLYQVF
                     AELASPPPPPANPPPGSIPAMTVSVPRALIERLSDRTPLVNGTPYDFWVTSASRPMLY
                     AVVEAAYGTVATTDKVSEQSVPADPNAYGATQVSGFSAPVTATPQPPDPFPRLEDTGG
                     CFIATAAYGSAMAPQVDVLRAFREQYLRPYALGRAVIRQYELWSPPLADAIRGSERGR
                     LMTRIVLWPVVGLAWLAVQWPLALILVLIVGAGGTLIWSVRGRMRRTTRA"
     gene            5347..6024
                     /locus_tag="AC16BC_07905"
     CDS             5347..6024
                     /locus_tag="AC16BC_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3241583.1"
                     /translation="MRKIVRLAVIAVALTGISFALPGPSVAAHGSRVVGIQVGYFVPA
                     TEGWKDGYDKRGGVTTGLTAGYALNAQWAIGAEAAYFSADNQARNASGQPSIEKQELT
                     LIPVTVGLEYRLEFSPEQVVVPFVGLGYRRVEYSLKVSSLSGIRGGAGGSVVRGGFDL
                     LLDALDPLSASGMQKEYGVAHTYLRFEAQLANVSARSEPDPTTLAPQPDIDLGGKTFL
                     LGLRFEF"
     gene            complement(6068..6955)
                     /gene="xerD"
                     /locus_tag="AC16BC_07910"
     CDS             complement(6068..6955)
                     /gene="xerD"
                     /locus_tag="AC16BC_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR3241584.1"
                     /translation="MDDAVKRFLQYLSVEKGLAPLTLEAYGRDLGRAVGLLTDQGVAT
                     WSAVDRNHLRACLSSLHRHGLSGRTIARTVTTLRGLYRFLLDEGAVSSDPTAWMESPQ
                     RPLRLPRSLSPGDVERLLACSLGDKPRHARDQAMIELLYATGMRVSELVSLKLTDVNL
                     QVGYVTPLGKGSKVRIVPVGDAGREKLTRYMSGARGALLDGRQSAHVFVSNRGDAMTR
                     QRFWQILRDYARAAGVSAKFSPHTLRHSFATHLLMHGADLRSVQAMLGHAQITTTQIY
                     THVTRERLKALHEQLHPRG"
     gene            complement(7139..7816)
                     /locus_tag="AC16BC_07915"
     CDS             complement(7139..7816)
                     /locus_tag="AC16BC_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3241585.1"
                     /translation="MAKKIRVLIADDHQVVRDGLSAILQTKDDIVVVGEAKDGVEAVE
                     KTKQLQPDVVLMDISMPRMTGVEATRKIKEQCPSVRVVVLTMYEEEEYIFDLVKTGVA
                     GYLLKNSDSAQIVKAIRSAAKGESFLHPTIAGKILTEFTHMAEGRGKKPAKGKSDLTE
                     REQGVLQYIAEGKTNKEIANALSISEKTVKNHVRNIFHKLDVYDRTQAAIQAIRRGII
                     RIDMQKS"
     gene            complement(7830..8861)
                     /locus_tag="AC16BC_07920"
     CDS             complement(7830..8861)
                     /locus_tag="AC16BC_07920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3241586.1"
                     /translation="MIHVAIIGAGQGGTSLIRILHDDPLVRIVGVADLDSNAPGMLLA
                     RQLKIRTTTDYRRLLKLPKVDTIIDVSGSDDVADALLALDRPEAAVVGGPGAKFMWQL
                     IDERIRSKEELERHLHEYQSLYRLYMKEVRHAISEERTRIALDIHDGLVQTLAGLSYK
                     MDLCREFLALDPARGARTLRETQHLLKGAIEEARQVVFSLKPLHFERLDLRTALKHYA
                     KTYAKQYHIATTVSVQGSERGLSPKTKIFLFRIVQEALSNVQKHAGASKVHVRVAIEK
                     TRLTASIHDNGRGFDLPGVSADPEKWASLGLKGMTERARLLGGRARIETQPGQGTTVS
                     VTIPLKHQE"
     gene            complement(8981..9463)
                     /gene="nrdR"
                     /locus_tag="AC16BC_07925"
     CDS             complement(8981..9463)
                     /gene="nrdR"
                     /locus_tag="AC16BC_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR3241587.1"
                     /translation="MRCPFCAEQEDRVVDSRVNRDGLMIRRRRECLGCQRRYTTYERV
                     EEILPAVVKKDGRRGPFDRAKILHGLKKACEKRPVPVSVLEDIADRVEKRVQEMGEAE
                     VASRVIGEEVMAQLHQVDQVAYVRFASVYREFKDVTQFLDELTTLLKHERRPDERPAP
                     "
BASE COUNT         1622 a         2913 c         3211 g         1736 t
ORIGIN      
        1 cgtcccgccg cgcgacacgg cgcccttggt tttgcggccg ccggcccgga cggaggggca
       61 tgccgtcacg atccgctccc ggagggtctt ggtttgactt tgccccggct gacctgctac
      121 tttacgccga tcggatacgg acgtccgttg ggggggcgcg atgaaagttg gcgttccgcg
      181 agaagtcatg gagcacgagt accgggtcgg catgaccccc gcgggggtgc gcgcggtgac
      241 ccggttgggc gcccaggtgt tcgtggaacg gggcgcgggc gagggcagcg ggtttcccga
      301 ccgcgcctac gaagaagcgg gcgccatgct cgtggcctcc acggaagcgg tctacaagat
      361 gggcgaattg atcgtgaagg tcaaggagcc gcttccgaag gaatacccct ggattcaaaa
      421 ggggcagacg ttgttcacgt tcctccacct ggccgcggat cgccgggtga ccgaggcgtt
      481 gttggatctc ggcgtcgtgg ggatcgcgta cgagaccgtg acgttggagg acgggacgcg
      541 gccgttgttg cggccgatga gcgatatcgc ggggcggctc gccgtgcagg tcggcgcctc
      601 gtacttgatg cggaccaacg ggggcagcgg ggtgctgctc tctggcgtgc cgggcgttga
      661 acgcggccgg gtgacgattc tcggcggcgg ggtggtgggc acgagcgcgg ccaagatcgc
      721 ggtgggactc ggcgcgcggg tcacggtgtt ggagcgggac ccgaatcgtc tgcacgtggt
      781 ggacgatctg atgggcgggc gcatcaccac cctgatcgcc agcgcggaca cgattcacca
      841 ggcggtggtg caggccgatc tggtggtggg cgcggtgttg gtgtcgggcg cgcgcacgcc
      901 gcacctggtc accggccaga ccgtggcgca gatgcgcaag gggtcggtga tcgtggacgt
      961 gtcgatcgac cagggcgggt gcgtggagac cgcgaggaaa accacgcagg cggacccggt
     1021 gtacacggcg cacggcgtga tccactacgg agtcgcgaac ctgcccggca cggtgccgcg
     1081 gacgtccacc caggcgttga ccgcggtgac gttgccgtac gtgatgcagt tggtcgagcg
     1141 cggcgttgag ggcgcgctca aggccgatcc cgcgttgggc gcggggttga atctgtatca
     1201 cggcaaggtg gtgcatgagg gcgtggccga ggcccatggg atgaagctgg aatcgtgggc
     1261 gtagtgatgg aatcggggaa cgggcgaatg ggggagccgg ggaaagatct ggtcggtgtt
     1321 cggcggttga attcccccct tctcccactc accggttccc cggttcgtat tttgaaaaaa
     1381 cggggtgtgg cgcagcctgg tagcgcacta gcttcgggag ctaggtgtcg gaggttccac
     1441 ccgagcttgc taagcgcaac gcagggccgg gttcccggcc cgaggctgcg agagggcttt
     1501 gacgttgggt cttgtgggtg acagatggga cggggtgtgg cgcagcctgg tagcgcacta
     1561 gcttcgggag ctaggtgtcg gaggttcaaa tcctctcacc ccgatttttt cgcggcgtga
     1621 tggccgaggc caaggtctac caagcgacgg tgaccgtgag cggccaggtg cagggcgtgg
     1681 ggtatcggaa tttcgccgcg gccgaagcca accgactggg cctcaggggg cacgtcaaaa
     1741 acctgcgcga tgggcgcgtc gaggtgcggt tggagggcgc gcgcgaggtg atcgacctcg
     1801 tgatcggccg gctccggatc ggacccacgg ccgcgtgcgt cgagtcggtg gacgtccggt
     1861 ggcaagaggg gtcgtcagga gcgagaggat tcactgttgc ctaatcccaa atcacgaaaa
     1921 gccgttcttc ccaaacgccc gcgtaaaccg aaagacgtca ctcttgacga cgaaccggtc
     1981 gaaccgtccg aatcagcctc cgacgaaggg ttccctctca agaccgaggt ggaggacgag
     2041 ggacatggcg aggccaacgg ggacgaggac gttgaattcg aagcgcgcga aaccgtcatc
     2101 gagccggagc ccgagtccga gcccggcggg gcgatggacg cggtcaagag ctacctcaag
     2161 gagattcgcc gctccacgct gctcacgttc gccgacgaac aacgcctcgc caagctgatc
     2221 gaaaagggcg acgccaaggc ccgcgagcag atgatcgaat ccaatctgcg cctcgtggtc
     2281 agcatcggca agcggtacat caatcgcggg ctcccgttct ccgacatcct ggaggagggc
     2341 aacatcgggt tgatcaaggc ggtggagaag ttcaactacc gccgcgggtt caagttttcc
     2401 acctacgcct cctggtggat ccgccagtcc atcgagcggg ccatcatcaa tcagagcaag
     2461 ttgatccgcc ttccggtcca cgtggtggaa aaggtcaacc attacctcca ggcggtggac
     2521 gcgctgatgc aggagtacga ccgggacccg accgccaagg aactctcccg gcgcctccgg
     2581 attccggtcg aggaagtggt ccatctgcag acgttgatcc gcaagacgta ctcgttggag
     2641 agcccgatcg ggagcgcgca ggaaaccacg ctcaaggaca tcatcgagga cacctcgcag
     2701 gtctccccgg cggccacgac cgaaggcatc cgtcggcgcg aggacatcat cgagtggatg
     2761 aaagtgctca aacccaacga gcagaaggtg ctgatcatgc ggttcgggct cgacggcgac
     2821 gagccccaca cgctggaaga aatcggcaag gtgttcggtc tcaccaggga gcgggtgcgt
     2881 cagatcgagg ccggggcgct cgccaagctc cgcggcgtga tcggcaagaa gagcatcacc
     2941 gccgagcaga tcatctagtc gaatcggttt ccgcgacgtg gacctcaaag ccaaaatccg
     3001 cgaagttccg aactttccca agcccggcat cctgttttac gacgtcacca cgctcctgcg
     3061 cgacgcggac gcgttcggcc gcgtgatcca ccgggtcgcc gagttctatc gcggcgaggg
     3121 gatcaccaag atcgtgggca tcgagtcgcg cggattcatc ctcggggcgc cggtggccgt
     3181 gcaactcggc gccggattcg tgcccgtgcg aaaaccgggc aaactgcccg ctgaggtcta
     3241 cgagaagacc tacgacctgg aatacggatc aagctcgctc gcgatccaca aggacgcgat
     3301 cgaccgtggc gagcgcgtgc tgatcgtgga cgacttgctg gcgaccggcg gcaccatggc
     3361 cgccaccgtg cacctggtca acgagctggg cggcgtggtg gtgggcgtcg cggtcctgat
     3421 cgaactggtc gaactgcgcg gccgcgacaa actgcgcgac cagcacgtcc tgtccatcct
     3481 cagttactga ggagcatgcc ctgatggggc gacatcgtcc gataccggtc ctggttgcct
     3541 ggggcctctg cgtcgcggcc atgacgtggc cgacgagcga tgcgttggcg aaagagatca
     3601 atgtcccagc cgacgccgcc tcgattcaag ccgccatcaa cctgtccaat caaagcaccg
     3661 acgcgagcga cacaatccac attgcaaccg gtacgtacat cgagaatctg acgatttcca
     3721 agcctacggc cgtcgccgcg aagtccctga ccatccgggc caatgacggc gccaaggtcg
     3781 tgctgcaagc ggcggcgcca gggtctccgc tcgttcaaat cggggcgggc gccgtggtga
     3841 cggtcgagcg cctgatcctc gtgacgtccg gcggtcgcgg cgtggtcatg gacgccgcga
     3901 ccgccagtct gcgcaacctc gtcatcaccc aagcgtcaac cgcgatcgag tgctcggtcg
     3961 cgacgtcggg gaataccgtc gagcaggtga cgttttcgca ggtggcaaac ggcatcaact
     4021 gcgtcagttc aacgctcacg attaggaaca acatcttcgc caacctctcc ggcattccga
     4081 tcgcgccgtt ctcccccgga tctggagcga ccctccctcg cttcaatttg ttcttccaga
     4141 gcggaaccag cggcgagcgc gggaccaacg aggtggtggc cagggccggt gacgatctcg
     4201 atccgcagtt cgtggacgta gccaacaacg atttccacat tcaggaaggg tccgcggccc
     4261 agggaaccgg cgaaggcggg gtggatgtcg gcgcctatgg cggcccgtcc agtcacacca
     4321 tcccgttccc gccgtcaaag ccttcgatca gcaactgcac gaccacaccc ccgctggcgt
     4381 gtaaggtcac ttgggcgaag aatctcgatt atctggtgac cgggtacgta gtgttgtcgc
     4441 acgcgacgac aacgcctacg gcgccgtcgt cgtcggacta cggctcaccc gtaccggtgg
     4501 acaacgcgac agccacatgc gaggtctcca acgacaccac gtgcgagacc acgctgagtt
     4561 cattgccgaa tttcacaacc actcctgctt cgccagtcca tctaagagca tcggtcggct
     4621 ccggaaaggt ccaattggcg tgggacaggg tttcgaactc caatctgtac caggtgtttg
     4681 ccgagttagc ttctccgcct ccgccgccgg cgaatccgcc cccaggcagc attccggcca
     4741 tgactgtctc agtcccacgt gccctgatcg aacgcctcag cgaccgcacg ccgctggtga
     4801 atggcacgcc ctacgatttc tgggtcacaa gcgcgagtcg gccgatgttg tacgcggtgg
     4861 tggaggccgc gtacggaacc gtggccacaa ccgataaagt gagcgagcag tcggtacccg
     4921 cagatcccaa tgcgtacggg gcaactcagg tcagtgggtt ttcggcgccg gtgaccgcta
     4981 cgccgcaacc gccggatccg ttcccgcgtc tggaggacac cggcgggtgt tttatcgcga
     5041 ccgcggccta tggctcggcg atggcgcctc aagtggacgt cctgcgggcg tttcgcgaac
     5101 agtatttgcg cccgtacgca ctgggtcgag ccgtcattcg ccagtatgaa ctgtggagcc
     5161 cgccgttggc cgatgccatt cgcggatcgg agcgcggtcg gttgatgacg cggatcgtct
     5221 tgtggccggt cgtggggttg gcgtggctcg cggtacaatg gccgctggcg ctgatcctgg
     5281 tgctgattgt tggcgcgggc ggaacactga tctggagcgt gcggggaaga atgcgcagga
     5341 ccacgcgtgc gtaagatcgt tcgtctcgcc gtgatcgccg ttgcgttgac cggcatcagc
     5401 tttgcccttc ccggtccgag tgtcgccgcc cacgggtcgc gcgtggtggg tattcaggtc
     5461 ggctactttg tgccggcaac cgaggggtgg aaggacggct acgacaaacg cggcggggtg
     5521 acgacgggtc tgacggccgg gtacgcgctg aatgcgcaat gggcgattgg cgcggaagcc
     5581 gcgtatttca gcgcggacaa tcaggcccgg aatgccagcg gacagccgag tatcgaaaag
     5641 caggagttga ccttgatccc ggtgacggtc gggctggagt accggctgga gttcagcccc
     5701 gaacaagtgg tggttccgtt cgtcggcctg gggtatcgcc gcgtcgagta cagtctcaag
     5761 gtgagttccc tcagcgggat ccgtggcggg gcaggcggct ccgtggtccg agggggattc
     5821 gatctattac tcgacgcgtt ggatccgttg tcggcatccg gcatgcagaa agagtacggc
     5881 gtcgcccaca cgtatctacg cttcgaagcc cagttggcca atgtcagtgc tcggtccgag
     5941 ccagatccca ccacgttggc gccccaaccg gacatcgacc tcgggggcaa gacgtttctg
     6001 ctggggctgc gcttcgaatt ctagtcggtg aggcgggcgg cgagtcggcg cccgccggct
     6061 tcccggacta tccccgcggg tgtaactgtt cgtgaagggc cttgagccgc tcccgggtca
     6121 catgggtgta gatctgcgtg gtcgtgattt gcgcgtggcc caacatcgcc tgaaccgatc
     6181 gcaggtcggc gccgtgcatg agcaggtgcg tggcgaacga atggcgcagc gtgtgcgggg
     6241 agaacttggc gctgacgccg gcggcccggg cgtaatcccg tagaatttgc caaaaccgct
     6301 gacgggtcat ggcgtcgccg cggttactca cgaacacgtg cgcggattgc cgtccgtcca
     6361 ggagcgctcc gcgcgcaccg ctcatatacc gcgtcaactt ctcgcgcccc gcgtcgccga
     6421 ccggaacgat gcgcaccttg gatcccttgc ccaacggggt gacgtatccg acctgcagat
     6481 tcacgtccgt caatttcaac gagaccagtt cggacacccg catcccggtc gcgtagagca
     6541 attcaatcat ggcctgatcg cgcgcgtgcc ggggcttgtc gcccagcgag cacgccagca
     6601 gccgttcaac gtcgccgggc gagagcgacc ggggcaggcg gagcggacgt tgcggcgatt
     6661 ccatccacgc ggtggggtcc gacgacaccg cgccttcgtc caagaggaac cgatagagtc
     6721 cgcgcagggt ggtgacggtg cgtgcgatcg tccggcccga caggccgtgg cgatggagcg
     6781 aggacaggca cgcgcggagg tggtttcggt caaccgcact ccaggtcgcg accccttgat
     6841 ccgtgagcaa gccgacggcc cgccccagat cgcggccgta ggcttccaac gtcaacggtg
     6901 cgaggccttt ttccaccgac agatattgga gaaaccgctt gacggcgtcg tccatcgtgg
     6961 ttaggggcga ccggcggtcg cccctaaccg gctgctgaaa aagtccatct gcggcgttgc
     7021 cgctgcgcat ccgctcctca cgtaccggag acgtacgctc cggtgcggtg ctcgcggcgc
     7081 cttgcatctg gagctttttg agcagccggt aagcttcata acctcattca acatcggact
     7141 acgacttctg catgtcgatg cggatgatgc cgcgacggat ggcctggatg gcggcctggg
     7201 tgcggtcgta cacgtcgagt ttgtggaaaa tgttgcggac gtggttcttc acggtctttt
     7261 cgctgatgga caaggcgttc gcgatttcct tgttcgtctt gccttccgcg atgtattgca
     7321 gcacgccttg ctcgcgctcg gtgaggtcgc tcttgccctt ggcgggcttc ttgccgcggc
     7381 cctcggccat gtgggtgaac tcggtcagga tcttgcccgc gatggtcggg tgcaggaacg
     7441 actcgccctt ggccgcggag cggatggctt tgacgatctg cgcggagtcc gagttcttga
     7501 gcaggtagcc ggccacgccg gtcttcacca ggtcgaaaat gtactcctct tcctcgtaca
     7561 tggtgagcac cacgacccgg acggacgggc actgttcctt gatcttccgg gtggcttcca
     7621 cgccggtcat gcgcggcatg ctgatgtcca tcagcaccac gtcgggctgc agttgtttgg
     7681 tcttctccac ggcttccacg ccgtctttgg cttcgccgac cacgacgatg tcgtccttgg
     7741 tctggaggat ggccgacagc ccgtcgcgca ccacctggtg atcgtcggcg atcaagactc
     7801 ggattttttt cgccacctgc gaatcctcct cattcctggt gtttcaaggg gatggtgacc
     7861 gagacggtgg tgccctgtcc gggttgcgtt tcgatgcgcg cgcgtccgcc gagcagacgg
     7921 gcgcgttcgg tcatgccttt gagacccagc gaggcccatt tttccgggtc cgcggacacg
     7981 cccggcaggt cgaacccgcg gccgttgtcg tgaatcgacg cggtcagccg ggttttctcg
     8041 atcgcgaccc gcacgtggac cttggacgcg cccgcgtgtt tctgcacgtt cgagagcgcc
     8101 tcctggacga tgcgaaagag aaaaatcttg gtcttgggcg agagcccgcg ttcgctgccc
     8161 tggacgctga cggtggtggc gatgtggtac tgcttcgcgt aggtcttggc gtagtgtttg
     8221 agcgcggtgc gcaggtccag gcgctcgaag tgcagcggtt tcaggctgaa gaccacctgt
     8281 cgcgcctctt cgatcgcgcc cttgagcagg tgttgcgtct ctcggagcgt gcgcgcgccg
     8341 cgggctgggt ccagcgccag gaattcccgg cacagatcca ttttgtagct cagtccggcg
     8401 agggtctgca cgaggccgtc gtggatgtcg agtgcgatgc gcgtgcgctc ctcgctgatc
     8461 gcgtgacgga cctccttcat gtacaggcgg tagagcgact ggtattcgtg cagatggcgc
     8521 tccaactctt ccttgctgcg gatgcgttcg tcgatcaact gccacatgaa cttggcgccg
     8581 gggccgccga ccaccgcggc ttcgggccga tcgagcgcga ggagcgcgtc cgccacgtcg
     8641 tcgctgccgc tcacgtcgat gatcgtatcc accttgggta gtttgagcag gcgccgatag
     8701 tcggtcgtgg tccggatttt cagttgacgg gcgaggagca tgcccggggc gttggagtcg
     8761 agatccgcca cgcccacgat cctgaccagc gggtcgtcgt gcagaatgcg gatcagcgac
     8821 gtgccgccct gccccgcgcc gatgatggcc acgtgaatca tgcggtccgc gggaacccgc
     8881 cgcgcggtgg gctttggaac ccgtggccgg ctcgggcggc ccacccgggg atccaggggc
     8941 atccggggcc tgatgactcc cgcgcgaagc gccgggcggt tcagggcgcg ggcctttcgt
     9001 cgggacggcg ttcgtgtttg agcaacgtgg tcagctcgtc gaggaattgg gtcacgtcct
     9061 tgaactcacg atacaccgag gcgaaccgga cgtaggcgac ctgatccacc tgatgcagct
     9121 gcgccatgac ctcttcgccg atcacgcggc tggccacctc ggcttcgccc atctcctgca
     9181 cccgcttttc cacccggtcg gcgatgtctt cgagcacgga aaccgggacc ggccgcttct
     9241 cgcaggcctt cttgagtccg tgaagaattt tggcgcgatc gaagggcccg cgccggccgt
     9301 cctttttcac gaccgccggc aggatttcct cgacgcgctc gtaggtcgtg taccgccgct
     9361 ggcaacccag gcactcgcgc cgccgacgga tcatgaggcc gtcgcggttg acccgcgaat
     9421 ccaccacgcg gtcttcctgc tcggcgcaaa agggacagcg catcagtgaa gaagaaaagc
     9481 ga
//
LOCUS       DBNGTT010000352         4138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2767756, whole genome shotgun sequence.
ACCESSION   DBNGTT010000352 DBNGTT010000000
VERSION     DBNGTT010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2767756"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1804)
                     /locus_tag="AC16BC_07930"
     CDS             complement(<1..1804)
                     /locus_tag="AC16BC_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241588.1"
                     /translation="MSDSERRATLRARIDVVSSKAASVFDDVVNAVESLLARDDVERW
                     VDLGCAIASGSGVTAVKFFRESPSLLERLPVDRRGAMIRVGRELARAQPNLSLEFLRQ
                     SSPLAERFDDAAIEQWGRIAADIAAQDYDASIEYVKESAALVGVLPTASFALWGEVGV
                     LLSREDRTVKDFLALKYFRSSADWLGAASDPALRPLVLALTCRVAAASFPSGATRRPG
                     QVALDILQYAHAMLGAVPSHETRHALLAAGARVADRAPTLVETFLRHAPAVVALVEGS
                     VTRFDEWVAEGLAIADSNRERAEAYFALRSKQATEVAQRLSRGVFLRDVRSTLTYVAE
                     AMCGRSVEIRQGAGASSALTERGGIITLPDKITVYATAEDNLRFYRVLTFHEAAHLEF
                     GTYEPVPAEVAAWFGAEGSAPAKTESSALLGRFPDPGLAQNLWTIAEEARVDFLIRHH
                     YPGLRSDMDRVVAEQLRARPRIDTLPRRAAILESWLQLSVADVADVPFPVLDPVTHGY
                     EVIKRLRKPEATVMDVLRAVVDLYPLAAEGMDEPLPVSSEAAPPAEPPSEFDQLAMGH
                     APIGSFAFRDALQAPQVRMGQHGGQPDQHGPRPPD"
     gene            complement(1797..2222)
                     /locus_tag="AC16BC_07935"
     CDS             complement(1797..2222)
                     /locus_tag="AC16BC_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241589.1"
                     /translation="MNTPSESWFSSRTAPAGSGSAEWPPYDIYAIGVIFILNVAMDTW
                     LIVKNPAYSLPLFGATFTGLPGWIAKIQSPILHGVLGYGFVRRRRWAYWLYMAYAAFG
                     LSSALVNLATIGFGRIRMIFIVSLAVITAYIVTRRKVFV"
     gene            complement(2292..3962)
                     /locus_tag="AC16BC_07940"
     CDS             complement(2292..3962)
                     /locus_tag="AC16BC_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241590.1"
                     /translation="MYRWLAYVCSLVWVLSIPGQQAHAANSFTVNETNQEGVSGTLLA
                     TPPGTCTGVPGGLPGDCSLRASAALRDWAEGYYGNGSRWSDATEAQLPSGTNRIGLDG
                     SVTPITSAALASLVNTRVGTSWIPEVCNRPWCGNLLRGDVISHLDENGDGKWDDTWID
                     KNFNGIREAGEVDRTRAEQFAWGITSVMREMSDLSLIVGNVSPPGISRMRTRIALGDA
                     SQTLNAGCDGDIMNPDCHNAVVTPPDTSGLTPTKVDDDSAVFGRCDPDRPEFVALMGD
                     PVRAQRALDNCLWFFASFPVTAPSPGDVDPLHNQLDPTLRPEGLDPLADPFAVRDTWI
                     NGRVVKYVSAVMPEQDFAQSWLVTYGFASPASTSDIGSAVYQNEWRMSQSDSGTDTYP
                     YSFLLQHTADGRRRGFTHDPDVPVDECAPPLPGDTCSGTTNPTVDPFWGKAIVRTAFD
                     LNGDGILDPDLNPDGSFKLNFDQNFEFFDGQPCDDACRESGVGREKAFTFLYEQEVEG
                     YLLSCLNCGHPAPEPAETVTYSFTWPGLPSVVPVLHPPLPSSTIPVLP"
BASE COUNT          766 a         1299 c         1389 g          684 t
ORIGIN      
        1 ggtcgggtgg gcgtggtccg tgttgatcgg gttgcccgcc atgttgtccc atccggactt
       61 gcggggcttg cagcgcgtca cggaatgcga atgacccgat gggcgcgtgg cccatggcga
      121 gttgatcgaa ctcggagggc ggttcggccg gcggcgcggc ctccgatgaa accggaaggg
      181 gctcgtccat gccctcggcc gccaagggat acagatccac cacggcgcgg agcacgtcca
      241 tgaccgtggc ctcgggtttg cggagtcgtt taatcacctc gtacccgtgc gtgaccggat
      301 ccagcaccgg aaacggcacg tccgccacgt cggccaccga gagctgcagc cacgattcga
      361 gaatggcggc gcggcggggg agggtgtcga tcctgggacg agcgcgcagt tgttccgcca
      421 cgacccgatc catgtccgac cgcagaccgg ggtagtgatg gcggatcaga aagtccaccc
      481 gggcttcctc cgcgatggtc cagagattct gcgcgaggcc ggggtcggga aagcgtccga
      541 gcagcgccga agactctgtc ttggccgggg ccgagccttc cgcgccgaac caggcagcaa
      601 cctcggcggg aacgggctcg taggtgccga actcgaggtg cgcggcctcg tgaaacgtca
      661 gaacccggta gaaacgcagg ttgtcttcgg ctgtggcgta gaccgtgatc ttgtccggca
      721 gggtgatgat tccgccgcgc tcggtcagcg cgctggacgc gcctgcgcct tgcctgattt
      781 ccacggaacg cccgcacatc gcctcggcca cgtaggtgag cgtgcttcgc acgtcgcgaa
      841 ggaacacgcc acgcgacagg cgttgcgcga cctcggtggc ctgcttcgag cgcagcgcga
      901 agtaggcttc ggctcgctcg cgattcgaat cggcgatggc gagtccttcc gccacccatt
      961 cgtcgaaacg ggtgaccgac ccttccacca acgcgacgac tgcaggcgca tggcgcagga
     1021 acgtctccac cagggtcggg gcgcgatcgg ccacgcgcgc gcccgccgcg agcaacgcgt
     1081 ggcgcgtctc gtgcgacggc accgccccga gcatggcgtg cgcatactgg aggatgtcga
     1141 gcgcaacctg accgggccga cgcgtggcgc cggatggaaa cgacgcggcg gccacgcggc
     1201 acgtgagggc caggacgagc gggcgcagcg cggggtctga cgccgcgccg agccagtcgg
     1261 cgctggatcg aaaatacttc agcgccagaa agtctttgac cgtgcggtct tctcgggaga
     1321 gcaggacgcc gacctcgccc cagagcgcaa acgacgcggt tggcagcacc ccgacgagcg
     1381 cggcgctctc tttgacgtat tcgatcgacg cgtcgtaatc ctgggccgcg atgtcggccg
     1441 cgatgcggcc ccactgttcg atcgccgcgt cgtcgaaccg ttccgccaac ggcgaggact
     1501 gtcgcagaaa ttccaacgac aaattgggct gggcgcgcgc gagttcccgt ccgacccgaa
     1561 tcattgcgcc gcgtcgatcc accgggagcc gttcgagcag cgaggggctc tcgcggaaga
     1621 atttgaccgc ggtcacgccg gacccggacg cgatcgcgca tcccagatcg acccaccgct
     1681 ccacatcgtc gcgggccagc aaggactcca ccgcgttgac cacgtcatcg aacaccgacg
     1741 cggccttgga cgagaccacg tcgatccgcg cgcggagcgt ggcccggcgt tcggaatcag
     1801 acaaagacct tcctccgcgt gacgatatac gccgtgatta cggcaagcga cacgatgaag
     1861 atcatccgaa tgcggccgaa cccgatggtc gccaggttga ccaacgcgct ggacagaccg
     1921 aacgcggcgt acgccatata caaccagtag gcccaccgtc gccgcctcac aaagccgtaa
     1981 ccaaggacgc cgtgcagaat gggcgactgg atcttggcga tccatcctgg aagaccggtg
     2041 aacgtggcgc cgaacaacgg gagagagtac gcgggattct tcacgatcag ccaggtatcc
     2101 atcgcgacgt tgaggatgaa gatgacgccg atcgcgtaga tatcgtacgg aggccattcc
     2161 gccgagcccg aacctgccgg ggcggtccgg gacgaaaacc aggattcaga cggcgtgttc
     2221 atcggggcga ctgacggacg ccccattgta gggatttggc ggcgggaaga caacgccgcg
     2281 ttgacgcgat gttacggcag aacagggatg gtgctcgatg gaagcggagg atgcagcacc
     2341 gggaccacgg acgggaggcc gggccacgtg aacgaatagg tgacggtttc cgcgggttcc
     2401 ggtgccggat gtccgcaatt caggcacgac agcaggtagc cctcgacttc ctgttcatac
     2461 aagaacgtga acgccttctc acggcccacg ccggattcgc gacaggcgtc atcgcacggt
     2521 tgtccgtcga agaactcgaa gttctggtcg aagttcaatt tgaacgagcc gtcgggattc
     2581 agatccgggt cgaggatgcc gtcgccgttc aagtcgaacg cggttcggac aatggctttg
     2641 ccccagaatg ggtcgaccgt gggattcgtc gtgccggagc aggtgtctcc cgggagcggg
     2701 ggcgcgcatt cgtccaccgg cacgtcgggg tcgtgcgtga acccgcgacg ccgtccgtcg
     2761 gccgtgtgct gcaagagaaa gctgtacgga tacgtgtcgg tgcccgagtc gctctgcgac
     2821 atccgccatt cgttctggta gaccgcgctg ccgatatccg atgtgctggc cggcgacgcg
     2881 aatccgtagg tcaccagcca actttgcgca aagtcctgtt ccggcatgac ggcggagacg
     2941 tacttgacca cgcggccgtt gatccacgtg tccctgaccg cgaacggatc ggccagcggg
     3001 tccagaccct cgggccgcag cgtgggatca agttgattgt gtagcgggtc aacatctccg
     3061 gggctcggcg ccgtgaccgg aaacgacgca aagaaccaca gacagttgtc cagggcgcgt
     3121 tgcgcgcgga ccggatcgcc catcagggcc acgaactcgg gacggtcggg atcgcagcgt
     3181 ccaaagaccg cgctgtcgtc gtccacctta gtcggcgtga gaccgctggt atccggcggc
     3241 gtcaccacgg cgttatgaca gtcggggttc atgatgtcgc cgtcgcaccc agcgttgaga
     3301 gtttgcgacg cgtcgccgag cgcgatcctg gtgcgcatgc gcgagatacc gggcggcgac
     3361 acgttcccga caattaagct caggtccgac atttcccgca tcacggacgt gatgccccat
     3421 gcaaattgct ccgcgcgcgt gcggtccacc tctccggctt cccggatgcc gttgaagttc
     3481 ttgtcgatcc aggtgtcgtc ccacttgccg tcgccgtttt catccaggtg cgagatcacg
     3541 tcgccgcgca ggagattgcc gcaccagggg cggttacaga cttccggaat ccagctggtc
     3601 ccgacccgcg tgttgacgag gctcgccaac gcggccgatg tgatgggcgt gacggaaccg
     3661 tccaagccga tgcggttggt cccgctcggg agctgggcct ccgtcgcgtc gctccatcga
     3721 ctgccgttgc cgtagtagcc ctcggcccag tcccgaagcg cggcgctggc tcggagcgag
     3781 caatctcccg ggaggccgcc gggcacgccg gtgcacgttc ccggtggggt ggcgaggagc
     3841 gttccggaga cgccttcctg gttggtttcg ttgaccgtga atgagttggc cgcgtgagcc
     3901 tgctgaccgg ggatactcaa cacccagacg agagaacaga catatgccag ccagcggtac
     3961 acgtgtccct acgtgggcgg acccttggtg gcggatgtgg tagaggttta tctgaggtaa
     4021 aaaaagcaat aagtgtgccg atgactctga gcgtcagaat gcttacgccg gaccgcagtc
     4081 gggaaacttc cgggaaaaag aaggaaggga aaggcctttt ccgccgaaca actactca
//
LOCUS       DBNGTT010000353         4455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2511025, whole genome shotgun sequence.
ACCESSION   DBNGTT010000353 DBNGTT010000000
VERSION     DBNGTT010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2511025"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="AC16BC_07945"
     CDS             <1..595
                     /locus_tag="AC16BC_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3241591.1"
                     /translation="VWPDLWLLPLDGTQEQRLISASPMVKEFVPALSSDGHWLAYLDL
                     PMDRPPQYRLMLVELPGGLPRMLTEDHVVFSPIRFSPDNRSILYTADTLGSSDVWTIG
                     VTGAGARARTTRRDAETAGDYSPDGATIVFVSNQSGTDELWLMDAQGLGARQLTYDLA
                     TASLPAFSPNGALIAYTSTKSGNQDIWVIGSGIDRRP"
     gene            768..1142
                     /locus_tag="AC16BC_07950"
     CDS             768..1142
                     /locus_tag="AC16BC_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR3241592.1"
                     /translation="MKRTVGIAVTAALLISAGWWLFAGKPVQGFYGDMVLNSKAEQRG
                     MPPVIFPHWIHRIEFKCKVCHPAIFEMKSGANDIDMEKIVFKGQFCGKCHNGETAWKP
                     VACARCHSGKPGMVTGPLHGVN"
     gene            1145..1759
                     /locus_tag="AC16BC_07955"
     CDS             1145..1759
                     /locus_tag="AC16BC_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR3241593.1"
                     /translation="MMRRAIGGLGVVVLATVALFACTKPFTKKADAPESAAPTAAATP
                     APPPAMPSPPMPTQLSEDFEDPTQMPTPGPPFARENRNPEVILKNVPRDQGGNVDFVE
                     AFKKELIHPRESLDPTKAPVPPFDFNIDIPAVGAMPNVVFPHFPHTYWLDCGNCHPNI
                     FVMKKGANPISMVKIVNGEFCGRCHGRVAFPISNCSRCHVKPKS"
     gene            complement(1836..3137)
                     /locus_tag="AC16BC_07960"
     CDS             complement(1836..3137)
                     /locus_tag="AC16BC_07960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR3241594.1"
                     /translation="MNICVIGSGYVGLVTGACFAEFGVTVTCVDADANKIERLKRGEI
                     PIYEPGLSELVQKGVREGRLSFTVDTADAVRQSLVIFIAVGTPPRGDGSADLSAVEAV
                     AETIARHMNGYKVIVTKSTVPVGTGDRVRAIIGKSQTERVNFDVASNPEFLREGAAIE
                     DFMRPNRVVIGAQSDQAIAILRDLYRPLYLIETPIVITDIATAEMIKYASNAFLATKV
                     SFINEIANLCEHVGADVHQVARAMGLDNRIGAKFLHPGPGYGGSCFPKDVVALLALAK
                     RNAYDFQILGAVDAVNHRQRDFMVEKICRAVGNVSGTTIACLGLSFKPNTDDIREAPA
                     LTILPALQKLGASIRAYDPAAMGHAESALPGVTMCHDPYEAISGCDAVVLMTEWNQFR
                     NLDLDRVKSLLRRPTFIDLRNVYDPARMREQGFSYVGVGRG"
     gene            3225..4169
                     /gene="rsmI"
                     /locus_tag="AC16BC_07965"
     CDS             3225..4169
                     /gene="rsmI"
                     /locus_tag="AC16BC_07965"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR3241595.1"
                     /translation="MPQPRAPDPTPADQRTSGNGPCGFVAGSDMASGLTSPVTSRDRA
                     SRLAVALRTLLRSRLVEGPPQQGVLYVVGTPIGNLEDITLRALRVLKEVDTVAAEDTR
                     HTQKLLARYELHATLTSYHDFNKETKTPILLARIREGASVALVCDAGTPTISDPGYFL
                     INRCIEAGVAVVPVPGPSAVIGALSASGLPTDRFHFEGFLPKTSGRLAKRLAALQNRP
                     ETIILFESPHRLLKTLNAIYTAWGNRPAVLARELTKVHEEFLRGTLAELIAELERRPR
                     RGEMTLLIGGCDRRSAGEPIAENDEPSPGSPTLHDAGR"
     gene            complement(4144..>4455)
                     /locus_tag="AC16BC_07970"
     CDS             complement(4144..>4455)
                     /locus_tag="AC16BC_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase response regulator"
                     /protein_id="HIR3241596.1"
                     /translation="DDFVLVCPPERVSGLCQQVIKEFDAAIPRFYDPGDQERGYIISR
                     DRNDVVQQFPIMTISIAVVTNERREITSPMQVAEIAAQLKKYAKSFPKSIFVIDQRRE
                     V"
BASE COUNT          866 a         1460 c         1314 g          815 t
ORIGIN      
        1 cgtttggcct gatctatggc tcctccccct tgacgggacg caggagcaac gcctgatttc
       61 ggcaagcccc atggtcaagg aattcgtgcc cgccctgtcg tcggacggac actggctggc
      121 ctatttggat ctcccgatgg accgtccgcc gcagtaccgc ctaatgctgg tcgaactccc
      181 tggcgggctt ccgcgtatgt tgaccgaaga ccacgtggtg ttttcgccga ttcgcttcag
      241 ccctgacaat cggtcgatcc tctacacggc cgacaccctc gggtcgagcg acgtgtggac
      301 gatcggggtc accggcgccg gagcgcgggc gcgcaccacg cgccgtgacg cggaaacggc
      361 cggcgactac agcccggacg gcgccactat cgtcttcgtc tcgaatcaga gcggcacgga
      421 cgaactctgg ttgatggacg cccaaggctt gggagcccgc caactgacct atgatctcgc
      481 gacggcctcg ctcccggcat tttcgccgaa cggcgccctc atcgcgtata cctcgaccaa
      541 gtccggcaac caggacatct gggtgatcgg ctcggggatc gaccgccgcc catgaccgac
      601 aaatggcgaa aacacgcgct cagccgtgcg tccggttatt gacaaagccc gcgcgcttat
      661 gctacgaatc tcgtccatcg cttcttccgt cggtgggcac ggcgtgcggg aagaacgatc
      721 gggatatccc tcaacagcaa cttgacgcct agcacagaga gcggcggatg aagcggacag
      781 tcggaatcgc ggtcacggca gcacttctca tcagcgccgg gtggtggctg tttgccggca
      841 aacccgtcca agggttttac ggcgacatgg tgctcaacag caaagccgaa caacgcggga
      901 tgcccccggt gatcttcccg cactggattc atcgcattga attcaagtgc aaagtgtgtc
      961 accccgcgat cttcgagatg aaatccggcg cgaatgatat cgacatggaa aagatcgtgt
     1021 tcaagggcca attttgcggg aagtgccaca atggagaaac cgcctggaaa ccggtggcct
     1081 gcgcccgatg ccactcagga aaacccggga tggtcacggg cccgcttcac ggagtgaatt
     1141 agccatgatg cgtcgcgcaa tcgggggact gggggtcgtc gtgctggcga ccgtggcgct
     1201 gtttgcctgc accaaaccgt ttaccaagaa ggccgatgct ccggaatccg ctgcgccgac
     1261 ggccgccgca acgcccgctc ctccgccggc catgccgtcg ccgccgatgc cgacgcagtt
     1321 atccgaggat tttgaagacc ccacccagat gccgacgccg gggcctccct ttgcgaggga
     1381 aaaccgcaat ccggaagtca tcctgaaaaa cgtcccgcgg gatcagggag gcaacgtcga
     1441 ctttgtggaa gcgttcaaga aagaattgat ccacccgcgg gaatcccttg atcccacgaa
     1501 ggcgccggtg ccgccgtttg atttcaatat cgacattcct gcggtgggag ccatgcccaa
     1561 cgtggtgttc ccccactttc cgcacactta ttggctcgac tgcggcaact gccacccgaa
     1621 catcttcgtc atgaaaaagg gcgccaaccc gatttccatg gtcaagatcg tgaacggtga
     1681 gttctgcggc cgatgccacg gtcgcgtggc gtttccgatt tccaattgct cacgctgcca
     1741 cgtgaaaccc aagagctgat tccccagaaa cacgaagggg ggagcgtcat caggcgctcc
     1801 ccccttcgtg tttctctacc ccacgcggcg cgcgtctacc ctcgcccgac tcccacatac
     1861 gaaaaccctt gctcgcgcat ccgcgccgga tcgtagacat ttcggagatc gatgaacgtc
     1921 gggcgacgga gcagcgactt cacccgatcc aaatccaggt tgcggaactg attccactcg
     1981 gtcatgagga ccacggcgtc gcacccggaa atggcctcgt agggatcatg gcacatggtc
     2041 accccgggca acgcggactc cgcgtgcccc atggcggccg gatcgtacgc gcggatcgag
     2101 gccccgagct tctggagcgc gggcaggatg gtcagcgcgg gcgcttcgcg aatgtcgtcg
     2161 gtgttgggtt taaatgacag ccccaaacac gcgatggtcg tgcccgagac attgccgacc
     2221 gcgcgacaaa tcttctcgac catgaagtcc cgttgacggt gattcaccgc gtcgacggcc
     2281 cctagaatct ggaagtcgta cgcgttgcgc ttggccaggg ccaagagcgc caccacgtcc
     2341 ttgggaaaac acgatccacc gtagcctgga ccgggatgga ggaacttggc tccgatccga
     2401 ttgtcgagtc ccatggcccg cgcgacctgg tgcacgtcgg cccctacgtg ctcgcacaga
     2461 ttcgcgatct cgttgatgaa cgacactttg gtggccaaaa acgcgttcga ggcgtatttg
     2521 atcatttccg cggtcgcgat gtcggtgatc acgatcggcg tttcgatcag atagagcggc
     2581 cggtaaaggt cccggaggat cgcgatggcc tgatcgcttt gcgcgccgat caccacgcgg
     2641 ttgggccgca tgaaatcctc gatcgccgcg ccttcgcgca aaaactcggg gttggaggcc
     2701 acgtcgaagt tgacgcgctc ggtctgcgac ttgccgatga tcgcccgcac ccggtcgccg
     2761 gtcccaacag ggacggtgct cttggtgaca atcaccttgt acccgttcat atgccgggcg
     2821 atcgtctcgg ccaccgcttc aaccgcggaa agatccgcgg agccgtcgcc ccgcggcggc
     2881 gtccctaccg cgatgaaaat caccagcgac tgccgcaccg cgtccgcggt gtcgaccgta
     2941 aacgagagcc gcccctcgcg cacgcccttc tgcaccagct ccgaaaggcc gggctcataa
     3001 atcgggatct cgccgcgttt gagccgctcg attttgttgg cgtccgcatc gacacacgtg
     3061 acggtcacgc cgaattcggc aaaacacgct cccgtcacaa ggccgacgta ccctgaaccg
     3121 atcacgcaaa tgttcactcg cgcctcctcg ctcgaccgac cgcgccctgc ggaagggcgc
     3181 tcgaccggtc atgtcggatt ggaactatag cagaggacgg ctcaatgcct caaccacgtg
     3241 cgcccgatcc aacgcccgcg gatcaacgga ccagcggcaa tgggccgtgc ggcttcgtgg
     3301 ccggctccga catggcctcc ggactgacgt ctccggtgac cagccgagac cgcgcgtcac
     3361 ggcttgccgt tgcgcttcga actctgctac gctcgcgcct tgtggaaggc cccccgcaac
     3421 aaggcgtgct ctacgtcgtc ggcacgccca tcggcaacct cgaggacatc acgttacgcg
     3481 cgctccgggt actcaaggag gtggacacgg tggcggcgga ggatacccgt cacacccaga
     3541 aattgctggc gcgatacgag ctgcacgcca cgctcaccag ctatcacgat ttcaacaagg
     3601 agacgaaaac tccgatttta ctcgcccgca tccgggaagg cgcgtccgtg gcgttggtct
     3661 gcgacgcggg tacgcccacg atttccgatc cgggctactt cctgatcaat cgctgtattg
     3721 aggccggggt agccgtagtc ccggttccag gcccgtccgc cgtcatcggc gcgctgtcgg
     3781 cttccggcct ccccaccgac cgattccact tcgaaggatt tttgcccaaa acctcgggcc
     3841 gactggccaa gcgcctggcc gcgctccaga atcgacccga gacgatcatc ctgtttgaat
     3901 cgccccaccg actcctcaag acgctcaacg ccatttacac ggcgtggggc aatcggccag
     3961 ctgttctcgc gcgggaactg accaaggtgc acgaggaatt ccttcgggga acgctcgccg
     4021 aactcatcgc ggaactcgaa cggcgtccgc gacgcggcga aatgaccctg ctgatcggag
     4081 gatgcgaccg gcgctccgcg ggtgagccaa tcgccgagaa cgacgagccc tcgcccggct
     4141 cacctacact tcacgacgct ggtcgatgac aaagatactc ttcggaaagc ttttcgcgta
     4201 tttcttgagc tgcgcggcga tctccgcgac ctgcatgggc gaggtgattt ctcgccgttc
     4261 gttcgtgacc acggcaatgg aaatcgtcat gatcggaaac tgctgaacca cgtcgttgcg
     4321 gtcgcgggag atgatgtatc cgcgttcttg gtcgccggga tcgtagaacc gaggaatcgc
     4381 ggcgtcgaat tccttgatga cctgttggca caggccggac acccgttccg gcgggcacac
     4441 caacacaaag tcgtc
//
LOCUS       DBNGTT010000354         3438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2130016, whole genome shotgun sequence.
ACCESSION   DBNGTT010000354 DBNGTT010000000
VERSION     DBNGTT010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2130016"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..822
                     /locus_tag="AC16BC_07975"
     CDS             31..822
                     /locus_tag="AC16BC_07975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3241597.1"
                     /translation="MIAWRMTAAAVVMAGGLVPAWAGDEVATVNGQPITKAAFDEAVA
                     GVPPQMQQRVTTVEGREALLDDLIMQEVLMQESKRAGVEKDPEVKRQLDDLRRQVLVQ
                     ATLKKVADTEVTDEKVKAYYDAHKDEFRQVRASHILVETEDQAKDARKRLAEGGDFAA
                     LAKELSTDPSAKENGGDLGFFQKDQMVKPFADRAFAMKVDEISDPVKTEFGYHIIKVA
                     EIKDAGPIDTLDPQALLDIKRSVLSKRIEDMKGRAKIVVHKDRLK"
     gene            complement(875..1192)
                     /locus_tag="AC16BC_07980"
     CDS             complement(875..1192)
                     /locus_tag="AC16BC_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR3241598.1"
                     /translation="MSDLQEKGYNKEEEYFYRQNKELLEKRRKELDAQRRQQESQQRH
                     AHWMKCPKCGADMREIERQGIKVDQCGGCGGIYFDRGELELLMEAKEQKGFLGGLKTL
                     FNK"
     gene            complement(1225..2175)
                     /locus_tag="AC16BC_07985"
     CDS             complement(1225..2175)
                     /locus_tag="AC16BC_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3241599.1"
                     /translation="MLPTGLVTDPGFLAHNTGPSHPESPRRLEAILRRLESTDLSKRL
                     VRLPAPLVDRRWLETVHTPRHVDRIESAAPEQGYVYLDADTPMSPGSYRAALQAVGGT
                     LAAIDAVMAGRIANAFCAIRPPGHHAEPARAMGFCLFNNVAVAARYVQRQHGLGRVLI
                     LDWDVHHGNGTQAAFYDDPTVLYVSLHQYPWYPGTGGADERGAGPGEGYTLNVPMAAG
                     RGDEEYLAAFDAQIAPAVRAFAPEFILISAGFDAHRDDPLAMMELTETGYRAMTEIVN
                     GWAAASANGRIVSCLEGGYNLTALADSATTHVRALAGLST"
     gene            2259..3023
                     /locus_tag="AC16BC_07990"
     CDS             2259..3023
                     /locus_tag="AC16BC_07990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3241600.1"
                     /translation="MPRLSLDRANDFLDYALSAPPTCGNPDTVVIYVHGFASHQRGEK
                     ATYFRERFVSQGWAYLTFDLRGHGQSSGTMAELTVTRALADVETILAWARERFPRIVV
                     IGSSLGGQLAAWAAARKPEAIAANLLIAPAFAFYENRVRDLGPEGEARLRAQGYAIVR
                     NEWVTTTIGRDLVEDASGYGLDRLLPQYETPTLILHGTEDSTVPFAGSVDFARRATAR
                     PLELVAIAGGDHRLTAHKAALFDHMIGFLGRLDGGR"
     gene            complement(2997..>3438)
                     /locus_tag="AC16BC_07995"
     CDS             complement(2997..>3438)
                     /locus_tag="AC16BC_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR3241601.1"
                     /translation="DIASKGGLGLTVHAGEDEGPDCIWQALDVLGVTRIGHGCSAVQD
                     PALVKRLAADRVMVECCLTSNYHTGAVKRGQPHPIFTFLEAGVPVAICTDNTTVSRTH
                     QTAENTKLAETLALNEIAAIHDRARAHSFIRDVRPASAAAVEPS"
BASE COUNT          594 a         1076 c         1176 g          592 t
ORIGIN      
        1 cgcggtcgcg cgaggaaggg gaggaacggc atgatagcat ggcgaatgac ggccgcggcg
       61 gttgtgatgg ccggtgggct ggttccggcg tgggcggggg atgaggtggc gaccgtgaac
      121 gggcagccga tcacgaaggc ggcgttcgac gaggccgtgg ccggcgtgcc cccgcagatg
      181 caacagcggg tgacgacggt cgagggccgc gaggcgctgc tggatgatct catcatgcaa
      241 gaggtgttga tgcaggaaag caagcgggcg ggcgtggaga aagatcccga ggtgaagcgt
      301 caactggacg acctgcggcg tcaggtcctg gtccaggcca ccctgaagaa ggtggcggat
      361 accgaagtca cggacgagaa ggtcaaggcc tattatgacg cgcacaagga cgaattccgc
      421 caggttcgcg cgagccatat tctggtggag accgaagacc aggccaagga cgccaggaag
      481 cgattagcgg aaggcggcga cttcgcggca ttggccaagg aactctcgac cgacccctcc
      541 gcgaaggaga acgggggcga tctcgggttc ttccagaaag atcagatggt caaacccttt
      601 gccgaccggg cgtttgccat gaaggtcgac gagatcagcg acccggtgaa aaccgagttc
      661 gggtaccaca tcatcaaggt cgcggagatc aaggacgcgg ggccgatcga caccttggat
      721 ccgcaagccc tgctcgacat caagcggtcg gtgctgtcca agcgcatcga ggacatgaag
      781 ggccgcgcca agatcgtggt ccacaaggat cgtctcaagt agcgcgcacc gtgtggcgcc
      841 ggaggggggc gccagccccc ctccggcccg cgcactactt gttgaacagc gtcttcaatc
      901 caccgaggaa gcctttttgt tccttggctt ccatgagcag ttcgagttcg ccgcggtcaa
      961 agtaaatccc gccgcaccct ccgcactgat caactttgat gccctggcgc tcgatttcgc
     1021 gcatgtccgc tccgcacttc gggcacttca tccagtgagc gtgtcgctgc tgggactcct
     1081 gctgtctgcg ttgggcgtcg agttccttgc ggcgcttttc cagcaattcc ttgttctgcc
     1141 ggtagaagta ttcctcttcc ttgttgtagc ccttctcctg caaatcagac atggatccgt
     1201 cctttcgtgt cgtggtgatg acgattacgt ggagagtccg gccaacgccc ggacgtgggt
     1261 ggtcgccgag tccgcgagcg cggtgaggtt gtacccgcct tccaggcacg agacgatgcg
     1321 gccgttcgcg ctcgcggccg cccagccgtt gacgatctcg gtcatggcgc gatagcccgt
     1381 ctcggtcagt tccatcatgg ccagggggtc gtcgcgatgc gcgtcgaatc ccgccgagat
     1441 caggatgaac tccggggcga acgcgcgaac cgcgggcgcg atctgcgcgt cgaacgccgc
     1501 gaggtattcc tcgtcgccgc ggcccgcggc catgggcacg ttgagggtgt agccctcccc
     1561 gggcccggcg ccgcgttcat cggcgccgcc ggttccggga taccacgggt actggtgcag
     1621 gctgacgtac aacacggtag gatcgtcgta aaacgcggcc tgcgtgccgt tgccgtggtg
     1681 cacgtcccag tccagaatca gcacccggcc gaggccgtgt tgtcgttgaa cgtatcgagc
     1741 cgccaccgcg acgttattga acagacagaa gcccatggcg cgcgcgggct cggcgtggtg
     1801 acccggcggc cgaatggcgc agaacgcatt ggcgattcgc ccggccatca ccgcgtcgat
     1861 cgcggcgagc gtgccgccca cggcttgcag cgccgcgcgg tacgagcccg gggacatcgg
     1921 ggtatcggca tccagataca catacccctg ctcgggcgcc gcggattcga tccggtcgac
     1981 gtgccgcggc gtgtgcaccg tttccaacca ccggcgatcg accagcggcg cgggaagacg
     2041 cacgaggcgt ttggagagat cggtggactc cagccgccgg aggatggctt ccaagcgcct
     2101 cggcgactcc ggatgcgacg gcccggtatt gtgagcgaga aatccgggat cggtgaccag
     2161 tccggtgggc aacacggcgc tactataccg ggaccatccc cgcgattcaa cgcaccgctg
     2221 cccttgcggc ggcccacggt ccgacctatg atacggctat gccgcggctt tccctggaca
     2281 gggccaacga cttcctggac tacgcgctca gcgcgccccc gacatgcggc aaccccgaca
     2341 cggtcgtgat ctacgtgcac ggattcgcgt cgcaccaacg cggagaaaag gcgacgtatt
     2401 tccgggaacg cttcgtctct caggggtggg cgtacctcac gttcgatttg cgcggccacg
     2461 gccagtccag cggcaccatg gccgagttga ccgtcacgcg cgcgctggcg gacgtcgaaa
     2521 cgatcctcgc gtgggcgcgc gaacgctttc cgaggatcgt ggtgatcggg tcgtcgttag
     2581 ggggacaact tgcggcctgg gccgcggcgc gcaagcccga ggcgatcgcc gcgaacctgc
     2641 tcatcgcgcc ggcgttcgcg ttctatgaga accgcgtgcg cgacctgggg ccggaagggg
     2701 aggcgcgctt gcgcgcccag ggctacgcca tcgtaaggaa cgagtgggtg accacgacca
     2761 ttggcagaga cctcgtggag gatgcgtcgg ggtacggcct cgaccgtctg cttccccagt
     2821 acgagacgcc gacgttaatt ctgcacggga ccgaggattc gacggtgccg ttcgcgggga
     2881 gcgtggattt cgccaggcgc gccacggcga ggccgttgga actggtcgcg atcgcgggcg
     2941 gagatcatcg tctcaccgcg cacaaggcgg ccctgttcga ccacatgatc ggattcttag
     3001 gacggctcga cggcggccgc tgatgcaggc cgcacgtcgc ggataaacga atgcgcccgc
     3061 gcccggtcgt ggatcgcggc gatctcattg agcgcgaggg tttccgcgag cttggtgttt
     3121 tccgcggttt gatgggtccg cgacactgtg gtgttgtcgg tgcagatcgc gaccggcacg
     3181 ccggcttcga ggaacgtgaa gatggggtgc ggctgcccgc gtttgaccgc gccggtgtgg
     3241 tagttggagg tcaggcagca ctcgaccatc acacgatccg cagccagtcg tttcaccagc
     3301 gccgggtcct gcaccgcgga acacccgtgg ccgatccggg tcacgcccag cacgtcgagc
     3361 gcctgccaga tgcagtccgg cccttcgtct tctccggcgt ggacggtcag tcccaatccg
     3421 cccttgctcg cgatgtcg
//
LOCUS       DBNGTT010000355         1951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3151488, whole genome shotgun sequence.
ACCESSION   DBNGTT010000355 DBNGTT010000000
VERSION     DBNGTT010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3151488"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="AC16BC_08000"
     CDS             <1..1091
                     /locus_tag="AC16BC_08000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3241602.1"
                     /translation="HAHGVLHRGVFYGNRQSEIPRELLRIIRLQPFQVLSSLPARYYY
                     PLKRIYLDWVTARLIRRDGCDVFHGWTTEALRSLQAARAVGAVSLIERPAPHPLVSQA
                     LLDEEYDLVGLPRPGRRIPRFLARIEASHRDRTVAVEEFALADRIIVQSDYSRESFLS
                     MGFSKDKVVLIPRAADTRRFVPGAGAGDVFRVLFVGQLCLRKGVHHLLRAWDRLALDR
                     AELWLVGSVHDEVRSLLKRYESNSSIRVFGHHPDPAALFQQSSVFVLPSIVEGSAKTT
                     YEAMACGLPIVVTPNAGAVARDGQEGFIVPIRDPNALAERIERLHRDRDLRRTMGQNA
                     RRLMESHTWDDLRRRMWSVYEDLVAGRA"
     gene            1088..>1951
                     /locus_tag="AC16BC_08005"
     CDS             1088..>1951
                     /locus_tag="AC16BC_08005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3241603.1"
                     /translation="MTSWRILHTESSTGWGGQEHRIVAEVEGMIGRGHRAWIAAPPEA
                     RILARARDRGIETIALPMQGPGMVRSIGGLVRLIRREGVQIVNTHSSRDSWLAAAAAL
                     VSSPRPVLVRTRHLSTPIRRSPVTRLIYNTLPDAVVTTGEEIRRTMIEEHGFDAGKIV
                     SIPTGIDTRRFTRTRYDRGFRRTLGVPDGAPLIGNVAVLRSWKGHEDFVDAARLVLDA
                     IPEARFVIVGDGPRLAVIREHIAARGLTDRVVMAGHRDDVPAVLAGLDVFVLSSFGHE
                     GVPQSVLQALAM"
BASE COUNT          278 a          630 c          694 g          349 t
ORIGIN      
        1 tccacgctca cggcgtgctc catcgcgggg tgttttacgg gaaccggcaa tcggagattc
       61 ctcgcgagct gctgcgcatc atccgtctcc agccgttcca ggtgttgtcc agtctcccgg
      121 cccggtatta ctacccgctc aagcggatct accttgattg ggtgaccgcg agactgatcc
      181 ggcgcgacgg ctgcgacgtg tttcacgggt ggacgaccga agcgctgcgc tcgctccaag
      241 cggcgcgcgc ggtcggggcg gtgagcctga tcgagcgccc cgcgccccat ccgctggtct
      301 cccaggccct gctggatgaa gaatacgacc tcgtgggcct tccacggccc ggccgtcgga
      361 ttccccggtt cctggcccgc atcgaggctt ctcatcgcga tcgcacggtc gcggtcgagg
      421 agttcgcgct ggcggatcgg atcatcgtgc agtcggacta ttcgagggag tcctttctga
      481 gcatggggtt ttccaaggac aaggtggtgt tgatcccccg cgcggcagat acccggcgct
      541 tcgtgcccgg cgccggagcg ggcgacgtgt tccgcgtgtt gttcgtcggc caactctgtc
      601 tccgcaaagg ggtgcaccat ctcttgcggg cgtgggatcg gttggcgctc gaccgcgccg
      661 agttgtggct ggtcgggagc gtccacgacg aagtccgctc gctgttgaaa cggtacgagt
      721 cgaattcatc gatccgggtc ttcggccacc acccagaccc cgcggccctg ttccagcaga
      781 gttcggtgtt tgtgttgccg tcgatcgtcg agggcagcgc gaagacgacg tacgaagcca
      841 tggcctgcgg gttgccgatc gtggtcacgc cgaacgcggg cgcggtggct cgggatgggc
      901 aggagggatt catcgtgccg atccgggacc cgaacgcgtt ggcggagagg atcgagcggt
      961 tgcatcgcga ccgggacctc cggcgaacca tgggccaaaa cgcgcgtcgt cttatggagt
     1021 cgcacacgtg ggacgacctt cgtcggcgca tgtggtccgt gtacgaggat ctggtcgccg
     1081 ggcgcgcatg acttcgtggc gcatcctcca taccgaatcc tccacgggct ggggcgggca
     1141 ggagcaccgg atcgtggccg aggtggaggg catgatcggt cgcggccatc gcgcgtggat
     1201 cgcggcgccg ccggaggccc gcattctcgc ccgcgcgcgc gaccggggga tcgagacgat
     1261 cgcgctcccc atgcagggcc ccggcatggt ccgctcgatc ggcggcttgg tgcggctgat
     1321 ccggcgcgaa ggcgtgcaga tcgtcaacac ccacagctcg cgcgacagtt ggctggcggc
     1381 cgccgcggcc ctcgtgtcga gcccccggcc ggtgttggtc cgcacgcggc atttgtccac
     1441 gccgattcgg cgttcgcccg tcacgcggtt gatctacaac acgctgcccg acgcggtcgt
     1501 gaccacgggc gaggaaatcc ggcgcaccat gatcgaagag cacgggttcg acgccgggaa
     1561 aatcgtgtcc atcccgaccg ggatcgatac ccggcgtttc acgcggacgc ggtatgaccg
     1621 gggatttcgt cgaaccctgg gcgtgccgga cggcgcgccg ctcatcggga acgtcgcggt
     1681 cctgcgaagc tggaaagggc acgaggattt cgtggacgcc gcccgcctgg tgctggacgc
     1741 gatcccggag gcgcgcttcg tgatcgtggg cgacggcccc cgcctggccg tgatccgcga
     1801 gcacatcgcg gcgcgcggat tgacggatcg cgtggtcatg gccggccatc gcgacgacgt
     1861 gcccgcggtg ttggcgggtc tggacgtgtt tgtcctcagc tcattcgggc acgagggcgt
     1921 gccgcagtcc gtgctgcagg ccctggcgat g
//
LOCUS       DBNGTT010000356         3726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1874047, whole genome shotgun sequence.
ACCESSION   DBNGTT010000356 DBNGTT010000000
VERSION     DBNGTT010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1874047"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="AC16BC_08010"
     CDS             <1..562
                     /locus_tag="AC16BC_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241604.1"
                     /translation="VIALLTGCAYGFNDHKKLGVVPDNPESATASFVFGYIDMADAEP
                     RLDWVTFQQVAPVVKDPSIPARAKDGVFYLENVSPGSYQVYDFGGRGGGFFAKGNLYA
                     TYPLSFPVSRQSGLLRVKVEKPGVYFLGSYKYVHVRGNRVGTNYVYELERLKAPTEKD
                     VLAQLLPAVKGTMWEPRLGDHLRKLK"
     gene            559..1182
                     /locus_tag="AC16BC_08015"
     CDS             559..1182
                     /locus_tag="AC16BC_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241605.1"
                     /translation="MTTGGRGWDRAFTWALLGCWVLLAGCFTGRTAVDSLAQVASGEA
                     VLVGRIELAPPLAEGEQQLKGLGTSGMKNRAVFITDDHWRDLPGELSMSDYNGYVGGE
                     FGQTFYVAVPTKPLYVLRGIMYIFLTGKRQEQAWLPGGLKVDVKPGDKAVYMGTIRYH
                     RNEFMDITKVEIKDNFGTESAAFKKKFGDAAVLRKRLAVVIAPPAKG"
     gene            complement(1213..2085)
                     /locus_tag="AC16BC_08020"
     CDS             complement(1213..2085)
                     /locus_tag="AC16BC_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3241606.1"
                     /translation="MERTIERVFRGAREHWVGNGFRVSNYFPSATDFEKRMSPFFLLD
                     YHAPRVYDPTTERRGVGTHPHRGFETVTIAYQGAVAHRDSTGAGGVIRPGEVQWMTAG
                     GGILHNEYHEETFAKAGGVMHMMQIWVNLPKRHKLTAPQYQPLTTADIPEVTLPNDTG
                     MVRVISGSYESTKGAASTFTPAHMLDLRLKAGAEVRLPTPKDYNTALLVLNGTVKAND
                     SAAVTDGQAVLFKNDGDEVRVHAVTDAIVLFLSGEPIEEPLVHYGPFVMNSVDEINQA
                     VEDFNAGRFGYLDD"
     gene            2363..>3726
                     /locus_tag="AC16BC_08025"
     CDS             2363..>3726
                     /locus_tag="AC16BC_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241607.1"
                     /translation="MTERADFSDHDGVWSQDRSVGISTQRYDADGWLIASDEHDPRVW
                     PHARTVVTREADGSITMETRIAGRDGKALKTYVSKYDSKRNRTGAREMDAGGSVQWNE
                     TVSRNAFGATIQERMAMDGSLLYRLSWFDTRHGFRMMDITNCREGLPASRSRQIYNEH
                     GQIMLADGYAGDGQPENRVVFDYGPDSNLLASTSYAPDGRVLSRRIETYDANRDRTSA
                     TEFAYDSSGGMPGRTEYTYGPDGLVSSISRSGMGGLNTGLQISYELDEQRNWTRQTTS
                     HCHAVGDESGRMWDDPKPCEVTIRTITYYPASVDTDSSARQGSLSIASSISDNEGGKS
                     SMSIGGGTLTGKVTFSGVPPAPKIFSLSKFSPPGYCERAESDGHGNRVIQEVNIGTDH
                     ALRDVVIAVQDVPMEQPVEFGGTKVINEGCKWLVQGGPSTLVGVVVKHKDFEVKNMDA
                     DPDDP"
BASE COUNT          745 a         1107 c         1162 g          712 t
ORIGIN      
        1 ggtgatcgcg ttgctgaccg ggtgcgcgta cggattcaac gaccacaaga aactcggcgt
       61 cgttccggac aacccggagt ccgccactgc atcattcgtc ttcggttata tcgatatggc
      121 tgatgccgag ccccggctcg actgggtaac gttccagcag gtggcgccgg tggtcaaaga
      181 cccgtcgatc cctgcgcgcg ccaaggacgg cgtgttttat ttggaaaacg tgtcgcccgg
      241 ttcgtatcag gtctatgact tcggcgggcg aggcggcgga ttctttgcca agggcaactt
      301 gtacgcgacc tatccgctct cgttcccggt gtctcgccag tccggtctcc tgcgagtcaa
      361 ggtagagaag cccggcgtct atttcctggg ctcttacaag tacgttcacg tcagggggaa
      421 tcgtgtcggc accaactacg tctacgagtt ggaacggctc aaggcgccga ctgaaaagga
      481 cgtgctcgcg cagttgctgc ccgccgtcaa gggcacgatg tgggagcctc gactcggcga
      541 ccatctaagg aagctcaaat gacgacaggg gggagaggat gggaccgggc gttcacctgg
      601 gcgctcctgg ggtgctgggt gctgctcgcc ggctgcttca cgggccggac agcggttgac
      661 agtctggccc aagtggcgtc tggagaagcc gtgctggtcg gacggatcga actggctcca
      721 ccgctcgcgg aaggagaaca gcagctcaaa ggcctgggaa cctccggcat gaaaaaccgc
      781 gcagtcttca tcacggacga ccattggcgc gacctgccgg gcgaactttc catgtccgac
      841 tataacgggt acgtcggcgg agagttcggc cagaccttct atgtcgcggt tccgaccaaa
      901 ccgctctatg tcttgagggg tatcatgtac atctttctaa cgggaaagcg ccaggagcag
      961 gcgtggctgc cgggcggact gaaggtggac gtcaaaccgg gcgacaaggc cgtatacatg
     1021 ggaacgattc gctatcatcg gaacgaattc atggacatta ccaaggtcga gatcaaggac
     1081 aatttcggaa ctgaaagcgc cgcattcaag aaaaagttcg gagacgccgc ggtcctccgc
     1141 aaacggctcg ccgtggtgat cgctccgccc gccaaaggct gatgagcggc gggcaagcga
     1201 tctcgcacgg cctcagtcgt ccagatagcc gaatcgtccg gcgttgaagt cttccaccgc
     1261 ctggttgatt tcgtccacgc tgttcatcac gaacgggccg tagtgcacga gcggctcctc
     1321 gatcggctcg ccgctcaaga acagcacgat ggcatcggtc accgcgtgca cgcgcacctc
     1381 gtcgccgtcg ttcttgaaca ggacggcctg tccgtccgtg accgcggccg aatcattcgc
     1441 cttcaccgtg ccgttcaaga ccagcagcgc ggtgttgtag tccttgggcg tgggcaaccg
     1501 cacctcggcg cccgccttca atcgcaagtc cagcatgtgc gcgggggtga acgtcgaggc
     1561 cgcgcccttg gtcgattcat acgagccgga aatcacccgg accatgcccg tgtcattcgg
     1621 cagcgtgacc tccgggatgt ccgcggtggt caggggctgg tattggggcg cggtcaactt
     1681 gtgccgcttg ggcagattca cccagatctg catcatgtgc atgacgccgc cggctttcgc
     1741 gaacgtttct tcgtggtact cgttgtgcaa aatgccgccg ccagcggtca tccactgcac
     1801 ttcgccgggg cggatcaccc cgccggcgcc ggtgctgtcg cggtgcgcga ccgcgccctg
     1861 gtacgcgatg gtcacggtct caaagccgcg atgagggtgc gtgcccacgc cgcgccgttc
     1921 cgtcgtgggg tcgtacacgc gcggcgcgtg atagtccaac aggaagaacg gactcatgcg
     1981 tttttcgaag tccgtggccg acgggaagta gttgctcacc cggaagccgt tgcccaccca
     2041 atgttcccgc gctccccgaa acacccgttc gatcgtgcgt tccatcggcg tctcctcccg
     2101 ctcccttgat atcacaaagc aaccgcttgt tccagcgaac acctaaatcg gattacacaa
     2161 cccacctttc ggtgttctcg ggactcgatt gacaccgtct tcggcggctt gatagggtag
     2221 gactacctcg tatcattcga gggacgccat gtgccaaatc gtagttttcg tggtcgtcct
     2281 gtttctgttc tccatcacac aacctgccga cgctcggtcc gacgcggagc ggcaggggtt
     2341 gaaggggcgc gtcaagcaag tcgtgaccga gcgcgccgac ttttccgatc atgacggcgt
     2401 gtggagtcaa gatcgcagcg tcggtatctc aacacaacga tatgacgcgg acgggtggct
     2461 catcgcgtcg gacgagcacg acccccgcgt gtggccccac gctcgcacgg tcgtcactcg
     2521 cgaagcggac gggtccatca cgatggagac ccgaatagca gggagggacg gaaaggcgtt
     2581 gaagacctac gtctcgaaat acgactcaaa gagaaaccga accggggcga gggagatgga
     2641 tgctggcggc tccgtgcagt ggaacgagac ggtctcgcgg aatgcgttcg gggcaaccat
     2701 tcaggaacgc atggccatgg atggctcctt gctctaccgc ttgtcatggt tcgacactcg
     2761 gcacggattt cgcatgatgg acatcacgaa ctgccgcgaa ggccttcccg cgtcgcgatc
     2821 acggcagatc tacaacgagc acggtcagat catgctggcc gacgggtacg ccggagacgg
     2881 gcaacccgag aatcgcgtgg tctttgacta cgggccagac agcaatctgc tcgcgtcgac
     2941 cagctatgcc cccgacggac gcgtgctctc gcgccggatc gagacctacg acgcaaaccg
     3001 cgaccgaaca agcgcgacgg agttcgcgta cgactccagt ggagggatgc ctgggaggac
     3061 ggaatacacc tacggtcccg acggcctcgt ttcctcgatt tcccgctccg gaatgggcgg
     3121 gctcaacacc ggtttacaaa tcagttacga actcgacgaa cagaggaatt ggactcggca
     3181 aaccacctcg cattgccacg cggtaggtga cgagtctgga cggatgtggg atgatccaaa
     3241 gccgtgcgaa gtaacgatac gcactattac ctactatccg gcatcggtcg atacggatag
     3301 ttccgcgcgc caaggctcgc tttccatagc gagttcgatt agcgacaacg aagggggcaa
     3361 gtcgtcgatg tcgattggtg gcggcacgct caccggcaag gtgacgttca gcggcgttcc
     3421 tcccgcgccc aagattttta gcctcagcaa gttctcgccc ccgggatact gcgagagagc
     3481 tgaatccgac ggccacggca accgggtcat tcaggaggtc aacatcggta cggatcatgc
     3541 gctcagggat gtcgtgatcg cggtccagga tgtgccgatg gaacagcccg tcgagtttgg
     3601 cgggaccaag gtgattaacg aggggtgcaa gtggctggtg cagggcggac catcaaccct
     3661 tgtgggcgtg gtggtgaaac ataaggattt tgaggtcaaa aatatggatg ctgaccctga
     3721 cgatcc
//
LOCUS       DBNGTT010000357         3881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_340239, whole genome shotgun sequence.
ACCESSION   DBNGTT010000357 DBNGTT010000000
VERSION     DBNGTT010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_340239"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="AC16BC_08030"
     CDS             complement(<1..146)
                     /locus_tag="AC16BC_08030"
                     /inference="COORDINATES: protein motif:HMM:NF022577.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR3241608.1"
                     /translation="MPKNVGGLDRFYRFGIGFLSLALVFVSHNLLLKIVFALVALLGI
                     ATAAT"
     gene            complement(200..1270)
                     /gene="aroC"
                     /locus_tag="AC16BC_08035"
     CDS             complement(200..1270)
                     /gene="aroC"
                     /locus_tag="AC16BC_08035"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3241609.1"
                     /translation="MAGNSFGQAFRISTFGESHGPALGVIVDGCPAGLPLAVEDIQRD
                     LDRRKPGQSLLTTQRQESDRVEILSGVFEGRTTGTPIAMLVRNEDSRSQDYSRIKDVF
                     RPGHADFTYWKKYVQRDYRGGGRASARETIGRVAAGAIARKLLAGRNISIIGYVIQIG
                     PVKIGKKDLSVINQNALFCPDPEAAERMIQVIKDAQAAKDSVGAMVELVARGVPAGLG
                     EPVFDKLDAELAKAMMSINAVKGVEIGAGFGVVEMRGSENCDPITPTGFASNHAGGIL
                     GGISNGDEIVIRMALKPTSSIAIEQDTIDIHGNPAKIATKGRHDPCVGIRAVPIGEAM
                     MALVLADHFLRDRTSRVDKLGT"
     gene            1338..1751
                     /locus_tag="AC16BC_08040"
     CDS             1338..1751
                     /locus_tag="AC16BC_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3241610.1"
                     /translation="MKSYREELWFQTPTRRQFINITPQVEAAIKKSGITEGLCLVNPM
                     HITASVYINDDESGLLADYEAFLELLVPQQGDYRHNLTGEDNADSHIKRQLMGREVVV
                     AITNGALDFGTWEQIFYAEFDGKRKKRVLVKIIGE"
     gene            1924..2094
                     /locus_tag="AC16BC_08045"
     CDS             1924..2094
                     /locus_tag="AC16BC_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241611.1"
                     /translation="MATCDTCGMLLLGYGNERDYEETRKALAEQGTPFSTMTQGALKV
                     VAKPRLAKSTRR"
     gene            2094..2510
                     /locus_tag="AC16BC_08050"
     CDS             2094..2510
                     /locus_tag="AC16BC_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3241612.1"
                     /translation="MTDLLAAEEAVLAANERFYRAFESLDIKEMESVWSTTETVQCIH
                     PGWGPLSGWSDVRDAWVRIFNNTSAMSFTPRVLHVAVQGETAWLVCVEEISRRHVDEE
                     HTSQVLATNVFERSAGQWRMIHHHASPIVRASQEES"
     gene            2512..>3881
                     /locus_tag="AC16BC_08055"
     CDS             2512..>3881
                     /locus_tag="AC16BC_08055"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIR3241613.1"
                     /translation="MTAAELFVRCLEAEGVQFIFGLPGEENLDLLHALSRSPIRFIPT
                     RHEQGAAFMADVYGRLTGRAGVCLATLGPGATNLATGIGDANLDHAPLVAITGQAGLE
                     RVHKESHQYVNIVEAFAPLTKWNTRVERASVIPEVVRHAFKTAQEEKPGACHIELPED
                     VAGEAVEGTPLASRRARRPSPDRPSLRAAADRIDAARHPIILAGNGVIRGNAARELVA
                     FAEKTSIPVANTFMAKGAMPGDHPLSLLSIGLQARDFVSCGFDAADLIITVGYDQVEY
                     APYHWNPNGDKPIVHIDFTPAETDAMYQPEVEVVSDVREALALLHGEVTQAHDRGYPA
                     RLREAILGDLDRDAGAGAMPMKPARVLRDLRRALGRRDVVISDVGAHKLLVARLFPAY
                     EPNTVLISNGFASMGIALPGAIAAALVEPDRRVVAVCGDGGFLMNVQELETAVRLAAA
                     FVVIVLR"
BASE COUNT          670 a         1214 c         1316 g          681 t
ORIGIN      
        1 gtagcggccg tggcgatgcc gagcagagcc acgagggcga agacgatttt caggagtaga
       61 ttgtggctga cgaacacgag cgcgagggag aggaagccga ttccaaatcg gtagaagcgg
      121 tccaggccgc cgacattttt aggcatctcg cctccttggg ttgatctttg gtcagaggca
      181 ctcccgacta tgtgtcgggt caggttccga gtttgtcgac ccggctcgtg cggtcccgca
      241 agaagtggtc ggccagcacc agggccatca tggcttcgcc gatcggcacc gcgcggatgc
      301 ccacgcaggg gtcgtggcgg cctttggtgg cgatcttggc ggggttgccg tggatgtcga
      361 tggtgtcttg ttcgatcgcg atcgatgacg tgggcttgag cgccatccgg atcacgatct
      421 cgtcgccgtt ggagatcccg cccagaatcc cgcccgcgtg attgctggca aagcctgtgg
      481 gcgtgatggg gtcgcagttt tcgctgccgc gcatttccac cacgccgaag cccgcgccga
      541 tttccacgcc cttgactgcg ttgatggaca tcatggcctt ggcgagctcc gcgtcgagtt
      601 tgtcgaacac gggttcgccc aagcccgctg gcacgccgcg cgccacgagt tccaccatgg
      661 cacccacgga atctttcgcc gcctgcgcgt ccttgatcac ctgaatcatg cgttcggccg
      721 cttccgggtc cgggcagaac agggcgttct ggttgatgac cgacagatcc ttcttcccga
      781 tcttcacggg gccgatttgg atcacgtagc caatgatcga gatgttgcgg ccggccagga
      841 gctttcgcgc gatcgcgccg gccgcgaccc gtccgatggt ctcgcgcgcg gacgcgcggc
      901 cgccgccccg gtagtcgcgt tggacgtatt tcttccagta cgtgaagtcc gcgtggcccg
      961 gacggaacac gtccttgata cggctgtagt cctgcgagcg cgagtcttcg ttgcgcacga
     1021 gcatggcgat gggcgtaccc gtggtgcggc cctcgaacac gccgctgagg atctccacgc
     1081 ggtcgctctc ttgccgctgc gtggtgagca ggctctggcc cggcttgcgg cgatccagat
     1141 cgcgttggat gtcctcgacc gcgagcggca ggccggcggg gcagccgtcc acgatcacgc
     1201 ccagcgccgg gccgtggctc tccccgaacg tgctgatgcg gaaggcttgt ccgaagctgt
     1261 tccccgccat tgcgttcccc ttgggtcggt ggcattatac acggccagta cggctcaaga
     1321 ccatccggat tgtccccatg aaaagttatc gcgaagaact ctggtttcag acgcccacgc
     1381 ggcggcagtt catcaacatc acgccgcagg tggaggcggc gatcaagaag agcgggatca
     1441 cggagggatt gtgcctggtc aacccgatgc acatcaccgc cagtgtgtac atcaacgacg
     1501 atgagtcggg cttgctcgcg gactacgaag cgttcttgga actgctggtg ccgcagcagg
     1561 gcgactaccg ccacaacctg accggcgagg acaacgcgga ctcccatatc aaacggcaac
     1621 tcatggggcg cgaagtcgtg gtggccatca ccaacggcgc gctggacttc gggacgtggg
     1681 agcagatctt ctacgcggaa ttcgacggca agcgcaagaa gcgcgtgctg gtgaagatca
     1741 tcggagagtg agaaggccat gaaccggaga gggggggaat cggcgaaccg gggaaaggac
     1801 gctggggata aagaatcgcc cgtacgctct gtcgccggtt cgccgattcc ccccacctcc
     1861 ggttcggtct tctccccgtg ttgtcaaacg tcggccaagc gctacatcaa gaaacaccgc
     1921 gacgtggcga cctgcgacac gtgcgggatg ttgttgctcg gctacgggaa cgagcgcgat
     1981 tatgaggaga cgcgcaaggc cttggccgag cagggtactc cgttctcgac catgacccag
     2041 ggcgcgctca aggtggtcgc caaaccgcgt ctggcgaaat caacgaggcg atgatgacgg
     2101 atctgttggc cgcggaagaa gcggtcctgg ccgcgaacga gcgcttctac cgcgcgttcg
     2161 aaagtctgga cattaaggaa atggagtcgg tatggtccac gaccgagacc gtccagtgca
     2221 tccatcccgg atgggggccg ttgtccggct ggtccgacgt gcgcgatgcc tgggtccgca
     2281 ttttcaacaa cacgtccgcg atgtccttca cgccccgcgt cctccacgtg gcggtgcagg
     2341 gcgagaccgc atggttggtg tgcgtcgagg agatcagccg ccgccacgtg gacgaagaac
     2401 acaccagcca ggtcttggcg acgaacgtgt tcgagcggag cgcggggcag tggcggatga
     2461 tccatcacca cgcgtcgccg atcgtccgcg cgtcgcagga agaatcgtag cgtgaccgcg
     2521 gccgagctct ttgtccggtg tctggaagcc gaaggcgtgc aattcatctt cggacttccc
     2581 ggcgaagaaa acctcgacct gttgcacgct ctgtcccgct ctcccatccg cttcatcccg
     2641 acccggcacg aacaaggcgc ggcgttcatg gccgacgtct acggacggct caccggccgg
     2701 gccggagtgt gtctggccac gctggggccc ggcgccacga acctggcgac cgggatcggc
     2761 gacgcgaacc tggaccacgc gccgctggtg gcgatcaccg ggcaggccgg attggagcgg
     2821 gtgcacaagg aatcgcacca gtatgtgaac atcgtggagg cgttcgcgcc gttgaccaag
     2881 tggaacaccc gggtggagcg ggcttcggtc attcccgagg tcgtgcgcca cgcgttcaag
     2941 accgcgcagg aggaaaaacc cggcgcgtgt cacatcgagt tgcccgaaga cgtggcgggc
     3001 gaggccgtcg aaggcacgcc gctcgccagc cgccgcgcgc gacggccgtc cccggatcgg
     3061 ccgtcgctgc gggccgcggc cgatcgcatc gacgcggcgc gccatcccat catcctggcc
     3121 ggcaacggcg tgattcgagg caacgccgct cgggagttgg tcgcgttcgc tgaaaagacg
     3181 tcgattcccg tggccaacac ctttatggcc aagggcgcca tgcccggcga tcacccctta
     3241 tcgttgctct cgatcggcct gcaggcgagg gatttcgtgt cctgcggctt tgacgcggcg
     3301 gacctcatca tcaccgtggg gtacgaccag gtcgaatacg cgccgtacca ctggaatccg
     3361 aacggcgaca aaccgatcgt gcacatcgac ttcacgcccg cggaaaccga cgccatgtat
     3421 cagccggagg tggaagtggt cagcgacgtc cgcgaggcgt tggcgctgtt gcacggcgag
     3481 gtgacgcagg cgcacgaccg ggggtacccc gcgcgactcc gggaggcgat cctcggggat
     3541 ctggatcgcg acgccggggc gggcgccatg cccatgaagc ccgcgcgagt gttgcgcgat
     3601 ctgcgccgcg cgctggggcg gcgcgacgtg gtgatcagcg acgtgggcgc gcacaagctg
     3661 ctggtggcga ggctgttccc ggcctacgag cccaacaccg tcctgatttc caacgggttc
     3721 gcgtccatgg gcatcgcatt gcccggcgcg atcgccgcgg cactggtcga gccggatcgg
     3781 agggtggtcg cggtctgcgg ggacgggggg ttcctcatga acgtgcagga gctcgaaacc
     3841 gcggtgcggc tcgccgcggc gttcgtcgtg atcgtgttgc g
//
LOCUS       DBNGTT010000358         3416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3406930, whole genome shotgun sequence.
ACCESSION   DBNGTT010000358 DBNGTT010000000
VERSION     DBNGTT010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3406930"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="AC16BC_08060"
     CDS             complement(<1..188)
                     /locus_tag="AC16BC_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241614.1"
                     /translation="MNDTSNSHRISRPGRVGFGAIVAVCLAAVLLALLEPGSTAFSQS
                     LAGGENSGALQSAGGFWRS"
     gene            complement(461..1075)
                     /locus_tag="AC16BC_08065"
     CDS             complement(461..1075)
                     /locus_tag="AC16BC_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014693.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HIR3241615.1"
                     /translation="MNASPSATTFDSVAAFHGHVCLDIALGYRVATAALRELACERPR
                     DEELVAIVENDSCSVDAVQAVTGCTFGKGNLVYHPNGKAAYTFVDRRTNRAVRIYCHF
                     WEAFDRGEGVAFAALMNRVLAGQASSAERERFGREMSEISRRILSLPEADLFSISRVD
                     TPPPPGARIFSSAACERCGEWTMETRLTAVHGKRLCPPCRDAGT"
     gene            complement(1072..1434)
                     /locus_tag="AC16BC_08070"
     CDS             complement(1072..1434)
                     /locus_tag="AC16BC_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241616.1"
                     /translation="MTTYLSAAFLQQCFAMHRYDLVVKRLTSTGVAVSCRQCKLRHAV
                     RLGPREAGDSVAVDQPAAVVGDGLLACAAGHLGDVCVQSVDVQRDHIELACRQCRSAD
                     AFRLVECVTRQLTGGGAP"
     gene            complement(1442..2971)
                     /locus_tag="AC16BC_08075"
     CDS             complement(1442..2971)
                     /locus_tag="AC16BC_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3241617.1"
                     /translation="MTALERKVQELTCLYEVARALATSLDIKETSGEVLRTLVTVLGA
                     TQGSLALRDPMTDDLVITAAHGLTPEEMARGRFKPTEGVTGAVFSSGEPMIVPDLGKE
                     PLFLNRTQSRADLSRVALVAVPVQAQGDVLGVLSVDRPKPDPSVSLDEDVRVLTVAAS
                     LIGQAVKLQRMVDEERRKLEAINLSLQHQLQAKYRASPIVGRSPAMIEVFEAIERVGG
                     SRATVLLLGESGTGKELIARAIHYNSPQAEGPFITLNCAALPEHLVESELFGHEKGAF
                     TGATALRKGRFELADGGTLFLDEIGELPLAMQSKLLRVLQERTFERVGGSRPITVQVR
                     LIAATNRDLERAVAEGTFRGDLYYRLKVVPISIPPLRERREDIPLLVDHFLKRFRQEH
                     QKPATIMPDALARLVEYPWPGNVREVENTMERLVIMGRQPQITLQDVVAVIGERARPV
                     PAAADSEGTLTSSVEELERQQIVKAMDEAGGVQAKAAKALGITPRQLGYRLKKYGLAP
                     S"
     gene            complement(2968..>3416)
                     /locus_tag="AC16BC_08080"
     CDS             complement(2968..>3416)
                     /locus_tag="AC16BC_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3241618.1"
                     /translation="AGLRVGYAVADPALIDRMERVRLPFNVNRPAVAALELVTTRRAW
                     FAATARRIVADRRKLAAELARFTRCRPLPSDANFLLLALPDGLSSASVMEGLLSRGII
                     VRDVSGMPGLGDGYLRVTVGTPADNRRLVRALAALPETAKASGRRR"
BASE COUNT          556 a         1219 c         1119 g          522 t
ORIGIN      
        1 gaccgccaga accctccggc ggactggagc gccccgctgt tctcgccccc cgccagcgac
       61 tggctgaacg cggtgctccc gggttcgagg agggccaaga ggaccgcggc aagacagact
      121 gcgacgatcg cgccgaaccc gacccggccc ggccgcgaaa tccgatggct gttggatgtg
      181 tcgttcatgc ccccctcaac gcgactgccg ttgtaccaca gcgcggccgg caaggaaaac
      241 ccctgctcgc cgtcccgcgt taccggaaag accagtcagc acgccacttt cctccgaatt
      301 cttttttgac gagaggggct ggtcggactg gatcaaccgc tgagggggaa cgccgccccc
      361 tgtttcaccg gggacgaggg ggccttctat aatgaggagc cgcgagcgga cgggcgatcc
      421 gctcaaaatc gtgcaatcga acggtgtcga gcggggctgg tcaggtcccc gcgtcgcggc
      481 agggcgggca aagtcgcttg ccgtggaccg cggtgaggcg ggtttccatg gtccactccc
      541 cgcatcgctc gcacgccgcc gaggagaata tccgcgcgcc ggggggaggc ggcgtgtcca
      601 cgcgcgaaat cgagaacaaa tcggcctcgg gcaacgacag gattcggcgc gagatctccg
      661 acatttcgcg gccgaatcgt tcgcgctcgg ccgacgaggc ctggcccgcc agcacgcggt
      721 tcatgagcgc ggcaaacgcc acgccctcgc cgcgatcgaa cgcttcccag aagtgacaat
      781 agatcctgac cgcgcggttg gtccggcggt ccacgaacgt gtaggcggct ttgccgttgg
      841 gatgatagac gagattacct ttgccgaacg tgcagccggt cacggcctgc accgcgtcca
      901 ccgaacaaga gtcgttctcc acgatggcga ccagttcctc gtcgcgcggg cgctcgcacg
      961 ccaactcgcg cagggccgcg gtcgcgaccc ggtaccccaa cgcgatgtcc agacacacgt
     1021 ggccgtggaa cgccgccacc gagtcgaacg tcgtggcgct cggcgacgcg ttcacggcgc
     1081 gccccctccg gtcaactgcc gggtgacgca ctcgaccagc cggaacgcgt ccgcggaccg
     1141 gcactgccga cacgccaact cgatgtgatc gcgctgcacg tccacgctct gcacgcacac
     1201 gtcgccgaga tggccggccg cgcacgcgag gagcccgtcg cccaccacgg ccgccggctg
     1261 atccacggca acggaatcac ccgcctcacg cgggccgaga cgaaccgcgt gccggagctt
     1321 gcactgccgg cacgacaccg ccacgccggt cgaggtcagc cgtttgacca ccaggtcgta
     1381 gcggtgcatg gcgaaacact gctggagaaa cgcggcgctc agatacgtcg tcatcgagtc
     1441 gtcacgacgg agccaggccg tatttcttga gccggtaccc caactgccgc ggcgtgatcc
     1501 ccagggcctt ggccgctttg gcctgcaccc cgcccgcctc atccatggct ttgacgatct
     1561 gttgccgttc gagttcttcc accgacgagg tcagtgtgcc ctcgctgtcc gccgcggccg
     1621 gcacgggtcg cgcacgctcc ccgatcacgg ccaccacgtc ctggagcgtg atctggggct
     1681 ggcgtcccat gatcacgagg cgctccatcg tgttctcgac ctcgcggacg ttcccgggcc
     1741 acgggtattc caccagccgc gccaacgcgt cgggcatgat cgtcgcgggc ttctggtgct
     1801 cctggcgaaa ccgcttgaga aagtgatcga ccaacaaggg gatgtcctcg cgtcgctccc
     1861 ggagcggcgg gatcgagatc ggcacgacct tcaaccggta atacagatcg ccgcggaacg
     1921 tgccctcggc caccgcgcgc tccaggtcgc gattggtcgc ggcgatgagg cggacctgga
     1981 ccgtgatggg ccggctgccg cccacgcgtt cgaacgtgcg ctcctggagc acccgcagga
     2041 gcttggattg catcgcgagc gggagttcgc cgatctcgtc caggaacaag gtcccgccgt
     2101 ccgccagttc gaagcgcccc ttgcgcagcg cggtcgcgcc ggtaaacgcg cccttctcgt
     2161 gtccgaacag ctcgctctcc accaggtgct cgggcaaggc ggcgcaattc agggtgatga
     2221 acggtccttc ggcttgcggg ctgttgtagt ggatggcgcg ggcgatgagt tccttgccgg
     2281 ttccgctctc ccccagcagc aacaccgtgg cccggctccc gcccacgcgc tcgatggcct
     2341 cgaacacctc gatcatggcc gggctgcggc cgacgatggg cgaggcgcgg tatttggcct
     2401 gcaactggtg ctgcagcgac aggttgatcg cttcgagctt gcggcgctcc tcgtccacca
     2461 tccgttggag cttgaccgcc tgcccgatca atgacgccgc caccgtgagc acgcgcacgt
     2521 cctcatccag gctgaccgac ggatcgggct tgggacgatc cacgctcagc acgccgagca
     2581 cgtcgccttg ggcctgcacc ggcaccgcga ccagcgcgac gcgggacagg tccgcgcgcg
     2641 actgcgtgcg gttcagaaac aacggctcct tgccgagatc gggcacgatc atgggttcgc
     2701 cgctggaaaa caccgccccg gtgacgccct cggtgggctt gaatcggccg cgcgccattt
     2761 cctccggcgt cagaccgtgc gccgcggtga tcaccaggtc gtcggtcatc ggatcacgca
     2821 gcgccaggct cccctgcgtg gcgcccagca cggtcaccag ggtccggagc acctcgccgc
     2881 tcgtctcttt gatgtcgagc gaggtggcga gcgcccgcgc gacctcgtac aggcaggtga
     2941 gctcctggac tttgcgctcg agcgcggtca tcgacgccgt cccgacgcct tggcggtttc
     3001 gggcaatgcg gccaacgcgc gaacgagccg ccggttgtcg gccggcgtcc cgacggtgac
     3061 gcggagatag ccgtcgccca accccggcat cccgctcacg tcgcgcacga tgatgccgcg
     3121 gctcaacagg ccctccatga cgctcgcgga ggacaagccg tccggcaacg ccaggagcag
     3181 gaaattggcg tccgaaggca gcggacgaca gcgcgtgaac cgcgcgagtt cggcggcgag
     3241 cttcctgcga tcggccacga tgcggcgcgc cgtggccgcg aaccacgcgc ggcgcgtcgt
     3301 gaccagttcg agcgccgcga ccgccggacg gttgacgttg aacggcagcc tgacccgctc
     3361 catgcggtcg atgagcgccg gatccgccac cgcgtacccc acgcgcagcc cggcca
//
LOCUS       DBNGTT010000359         1801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3280019, whole genome shotgun sequence.
ACCESSION   DBNGTT010000359 DBNGTT010000000
VERSION     DBNGTT010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3280019"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1697)
                     /gene="serA"
                     /locus_tag="AC16BC_08085"
     CDS             complement(117..1697)
                     /gene="serA"
                     /locus_tag="AC16BC_08085"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3241619.1"
                     /translation="MRVLVSDSLSTQGVEILKQAGLDVDVKTKLTPEQLLDEIKNYEG
                     LVVRSATKVTAKVLDAGVKLKVVGRAGSGLDNVDLDAATKRGVVVMNTPGGNTVTTAE
                     HAIAMLCALARQIPQATASTKGGKWEKSKFMGVELYQKTLGVIGLGQIGSHVAKLGQG
                     LMMKVLAYDPYLSEDRARQMGVEHATLDQVFRRADFITIHSPLTPETKNLVRAETIAT
                     MKDGVRIVNCARGGIVNEADLYQALSSKKVGGAALDVFEKEPVDPANPLLTLENFICT
                     PHLGASTTEAQENVAVAVAEQIVDLLVKGIVRNAANIPSVPSDLLPQIQPYLALGEKL
                     GAFEAQRLDGGIRRVTVEYHGEVATLPTAPITVAILKGLLNPILEASVNYVNAPVVAK
                     ERGIELNEVKRTDAGDFSSLVVVELQVGDRTSRIAGTLFHRREPRIVSIDDFSVEVVP
                     EGAMLALSNSDQPGVIGTIGTFLGRHQVNISRMQLSRERPGGRAISVIGIDSPVSAST
                     LQELRELPGILSAKQIRL"
BASE COUNT          313 a          598 c          541 g          349 t
ORIGIN      
        1 agaaggggtc agatgcaagg cggagcaaga agccgtaccg ccgcgtaccg ggtggtacgc
       61 gaggatacgg attcgcagcg acaacgccgc agatggcccc ttctcgtcag atcagatcac
      121 aaccgaatct gtttggcgga gagaatgccc ggcagttcgc ggagctcctg cagcgtggac
      181 gcggacaccg gcgaatcgat gccgatcacc gaaatcgcgc gcccgcccgg ccgctctcgc
      241 gacaactgca tgcgggagat attgacctga tggcgaccga ggaatgtacc gatggttccg
      301 atgacgccgg gctgatcgct gttcgagagg gccaacatcg cgccttccgg gaccacttcc
      361 acggaaaagt cgtcgatgct cacgatgcgt ggctcgcgcc ggtgaaacag ggtacccgcg
      421 atgcgagagg ttcgatcgcc gacctggagt tccacgacca ccagactcga aaaatcgccc
      481 gcgtccgtgc gcttcacttc gttcaactcg atcccccgct ccttggcgac gaccggcgcg
      541 ttgacgtaat tgacgctcgc ctcgaggatc ggattgagca ggcctttcag gatcgccacc
      601 gtgatcggcg cggtgggcag ggtggccacc tcgccgtggt attcgacggt cacccgccgg
      661 atgccgccgt cgagtcgctg cgcctcgaac gcgccgagtt tttcgcccag cgccaggtac
      721 ggctggatct gcggcagcag atccgacggc accgagggaa tattcgccgc gttgcggacg
      781 atgcccttga ccaggaggtc cacgatctgc tcggccaccg cgaccgccac gttttcctgc
      841 gcttcggtgg tcgacgcgcc caagtgcggc gtgcagatga aattctcgag cgtcaacagc
      901 ggattggccg gatcaaccgg ctccttctca aaaacgtcga gcgccgcgcc gcccaccttc
      961 ttgctcgaca gcgcttgata gagatccgcc tcgttgacga tcccgcctcg cgcgcaattg
     1021 acgatgcgca cgccgtcctt catcgtcgcg atcgtctcgg cgcggaccag gttcttggtt
     1081 tccggcgtca ggggcgaatg gatcgtgatg aaatccgccc gacggaacac ctgatcgagc
     1141 gtggcgtgct cgacccccat ctgccgggcg cggtcttcgg acaaatacgg atcgtacgcc
     1201 aacaccttca tcatcaggcc ttggcccagt ttggccacgt ggctgccgat ctggcccagg
     1261 ccgatcaccc cgagcgtttt ttggtacagt tcgaccccca tgaacttgga cttctcccac
     1321 ttcccgcctt tggtcgaagc cgtggcttgg ggaatctgcc gcgccagcgc gcagagcatg
     1381 gcgatcgcgt gttcggcggt ggtgaccgtg ttgccgcccg gcgtgttcat gaccaccacg
     1441 ccgcgtttgg tggcggcgtc gaggtccacg ttgtcgaggc ccgatcccgc gcgccccacc
     1501 accttgagct tcacgcccgc gtcgagcacc ttggccgtga ctttcgtggc gctgcggacc
     1561 accagccctt cgtagttttt gatctcatcc agcaactgtt cgggcgtcag cttggtcttg
     1621 acgtcgacgt cgaggccggc ttgcttgagg atctccaccc cttgagtgga caggctatcg
     1681 ctgacgagca cacgcatagt gtttcagtgg ctgtgaatcc tattgcacca aaatcccttg
     1741 agcgaccccg acgcccttcc cgagcgtcac ggagtgcccc atccctttca acaccatctc
     1801 c
//
LOCUS       DBNGTT010000360         9558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1364242, whole genome shotgun sequence.
ACCESSION   DBNGTT010000360 DBNGTT010000000
VERSION     DBNGTT010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1364242"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="AC16BC_08090"
     CDS             complement(<1..471)
                     /locus_tag="AC16BC_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstC family ABC transporter permease"
                     /protein_id="HIR3241620.1"
                     /translation="MIARTGTKHVQEQAVHAVLLLCGALSIITTLGIVLVLLVETVAF
                     FREVSVVDFLTDTQWTPLFADKHFGILPLVCGTVLTAGIAIVVALPVGLVSAVYLSEY
                     AGERLRRVVKPVLVVLAGIPTVVYGYFALLFVTPLLQLVIPTLSGFNALAPGIVI"
     gene            complement(510..1526)
                     /locus_tag="AC16BC_08095"
     CDS             complement(510..1526)
                     /locus_tag="AC16BC_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3241621.1"
                     /translation="MSRRNGWGIGLAALLGVWTTAFAGSAHAATLIKVDGSSTVYPIT
                     EAVAEEFQKKNRTVRITVGISGTGGGFKKFCGGETDLADASRPIKATEVKQCADNKVE
                     YIELPVAFDGLAVMVNPKNTWAASMTVAELKKLWEPPAQGKITRWSQIRAGWPDKEIR
                     LFGPGVDSGTYDYFTEAVVGKEHASRGDYTASEDDNVLVQGIAGDPLALGFFGVSYYD
                     SNKGRLKLVAIDDENPKNGAGPILPTAETIKNATYEPLSRPIFIYVNKKALDRPEIAA
                     FVKFYLTAGRELVNEVGYIPLPDKAYELAMKRVEARAVGSVFGGKGSQVGVTIESLLA
                     VETK"
     gene            complement(1582..2829)
                     /locus_tag="AC16BC_08100"
     CDS             complement(1582..2829)
                     /locus_tag="AC16BC_08100"
                     /inference="COORDINATES: protein motif:HMM:NF028240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HIR3241622.1"
                     /translation="MKRWIGSLVVAPWALLAATLAGAEERKPLDQLLVEKGVLTKEEA
                     ASVQGAKLAAWLDRITFGGDIRLRQEMVYRHNGADDRSRQRFRLRVGADLKIDTVVVG
                     VRLASGTGEHVSANKSFDDLSAPTPLYIDRAFVRWQGDDTRWLTVTGGRMGNPFFSVY
                     SSDLVWDDDYNPEGLAENLSFALGGGDVFVNLAQIVLDEDSAGSLSTDQWLFGQQVGV
                     KMNPTTTLQTALAVTYLNAVNTQNGTLDQSVCADGNSRQATGTLCPGSTTRNAELVND
                     YDVVHVTAAATTKLASLPVSVMGDAVKNTADTQDASGNDTKDGGYQVGVILGKAGDPR
                     TWEVAYFYKKVGTDATLADIADSDFGTDGGTDRKGHIMWAAYNPTQAIQVKSKYMTSE
                     VEDDSSAEQKDVSRLQIELSVKF"
     gene            complement(3041..3526)
                     /locus_tag="AC16BC_08105"
     CDS             complement(3041..3526)
                     /locus_tag="AC16BC_08105"
                     /inference="COORDINATES: protein motif:HMM:NF017913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HIR3241623.1"
                     /translation="MTLSTTLDEVWQRLPAIAGLDAPRVWTTAIKAVLSLLLGLILLT
                     LLVGVVSVGLELRELGTAGVDAVLQRVLIGVLMLLAIVEVFRTTLAYLAEGRVKVTFI
                     VDTILVVMLTEILSLWLKGGEGATFTLLLFVIAVLSGVRILAIRFSPSSPGPDLRRDA
                     E"
     gene            3695..4873
                     /locus_tag="AC16BC_08110"
     CDS             3695..4873
                     /locus_tag="AC16BC_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR3241624.1"
                     /translation="MEPLWLIAALLVALILVVGVALFRRRPDPTITLLQQQLDAMSRQ
                     LSESAGGSKLMHDRLGQVTQEVNGQLSAITTQVNGQLGLITAQVNQQLTAIGKQVQEA
                     TGQMGTRLDNAARVVGEVRQNLGELTKATERVFDVGKDIASLQEILRAPKLRGVLGEL
                     FLGDLLGQILPPTHYALQYKFKSGEAVDAAIHLGQGIVPVDSKFPLENFRRVIASEAE
                     DERKAAKKKFVIDVKKHIDAIASKYILPDEGTFDFALMYIPAENVYYEVILKDDAFGE
                     DKGLCAYALAQRVIPVSPNSFYAYLQAIVLGLKGLRIEKNAQEMIQHLKRLQGDFQRF
                     RDDFDVLGKHLGNTRGKYEDASKRLERFGEKLLSVEESAAAPTSIEVDAASPAQRPLL
                     "
     gene            complement(4877..5170)
                     /locus_tag="AC16BC_08115"
     CDS             complement(4877..5170)
                     /locus_tag="AC16BC_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR3241625.1"
                     /translation="MKHCPYCAEEIQDDAIKCRYCGSFLNPTGNPRRLTRSRRERKLA
                     GICGGMAGYLGIDPAIVRILWVLAAFISFGVVVLLYLALIFIVPNEDEPERSV"
     gene            5322..6221
                     /locus_tag="AC16BC_08120"
     CDS             5322..6221
                     /locus_tag="AC16BC_08120"
                     /inference="COORDINATES: protein motif:HMM:NF021454.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HIR3241626.1"
                     /translation="MNECSRWIAGILLCLVIGGGVGSAHADEAERDLAWMVGPARVPL
                     ADQAVLTLPEGYRAIGPDDTQYVLKEMGNYPSGTELALIAPSADSPDSWFVVVRYVDA
                     GYIRDDEATDWDADGLLTSIREGTDEDNRQRKDAGQAALLIRGWEQKPAYDKASNKVV
                     WAISHTVEGEEGVGVNYNTLALGRHGYMSMNMVGDLNELAAMKPKAELLLTAMSFVDG
                     KRYADFNGATDKVAAVGLTALVAGVAAKTGLLAKLWAFLLPVVLVAKKFIVLLVVAII
                     ALGRSLWKRMRANAGPPTASPAP"
     gene            6167..6922
                     /locus_tag="AC16BC_08125"
     CDS             6167..6922
                     /locus_tag="AC16BC_08125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3241627.1"
                     /translation="METDAGERGTADRVARPVKTILLLLGAAKLGKVALTGGTMLLSM
                     VTYSFVFGWRYAVGFVLLIFFHESGHYVAAKQRGLNVGAPTFIPFVGAWIQLKDQPHD
                     VETEAYVGIAGPVAGTIAALACYYASEALGNRLLLALAYAGFMINIFNLIPLSPLDGG
                     RITAVLSPRVWWIGAPILVGLFMVRPSPMLILVAILAAPHLLATMGRGTGPMPARYYA
                     VSPRTRLTYGAYYLGLAAFLGVMTYETHHMLGA"
     gene            6983..7957
                     /locus_tag="AC16BC_08130"
     CDS             6983..7957
                     /locus_tag="AC16BC_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019889.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HIR3241628.1"
                     /translation="MIERRPMFGVPTLSIALALFAALTVPATTTAADVFTWTDENGTI
                     HFSDSVSDVPAKYRKSIKQKKFGPDTDSPSAAPKTPAQPPDQAAAPSPEPAPKDPSAP
                     ARFEVPYTPYEGSARRLIVSVRFNNRVTAPMILDTGAPSSLISMDIAEELGLLDKEHG
                     RLLVNIGGIGGSAVAVKTILDRVQIGDAKIDFVPAEITSSISDKFEGLIGMDFMANYS
                     TQIDSKKRVVVFEQIDDDASRPGGYDETWWRNTFREFADSYNRWKDYLQRLDKEIEQS
                     PIVNRGMPTDLSRRRDFAYWQTQEAEKLMDRLHRFARENAVPMHWRTY"
     gene            complement(7999..8385)
                     /locus_tag="AC16BC_08135"
     CDS             complement(7999..8385)
                     /locus_tag="AC16BC_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3241629.1"
                     /translation="MAIRIVRLGTPRAPNEGLRIGTVRRPPRGVPKSKFSSENWYDVW
                     LSNVAPTQETVTLALNAKTERQWAGFVKRYRAEMKKPENSRVLDLLAALSHGAQFSVG
                     CYCEDESRCHRSVLRELLLERGATLV"
     gene            complement(8739..9212)
                     /locus_tag="AC16BC_08140"
     CDS             complement(8739..9212)
                     /locus_tag="AC16BC_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241630.1"
                     /translation="MAILKADPRLRRQILLLHIAFAVLTFGTVQWLFPMFEEYLKKQE
                     LWVATRSFQFMIAILFAPVLGMAYYMHRVAKQILASGQCPPPGTRVVRDTEILEVDAA
                     RRRGRLLLILSVVLAGAALVGMIYFPYQIGEVFGKFERQPQPDASLQQTSVELNH"
     gene            9452..>9558
                     /locus_tag="AC16BC_08145"
     CDS             9452..>9558
                     /locus_tag="AC16BC_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3241631.1"
                     /translation="MLGLVLTGPPEALAIFQYTDDAGTVHFTDDPGQIP"
BASE COUNT         1870 a         3125 c         2790 g         1773 t
ORIGIN      
        1 gatcacgatc cccggcgcga gcgcgttgaa cccggagagc gtggggatga cgagttgcag
       61 cagcggggtc acgaacaaca acgcgaagta gccgtacacc acggtgggga tgcccgccaa
      121 caccaccaac acgggcttga ccacgcgacg caggcgttcg cccgcgtatt cgctcaagta
      181 caccgcgctc accagtccga ccggcaacgc caccacgatc gcgatccccg cggtgagcac
      241 cgtgccgcag accagcggta ggatgccgaa gtgcttgtcc gcgaagagcg gggtccactg
      301 cgtgtccgtg agaaaatcga ccaccgagac ttcgcggaag aacgccacgg tctccaccaa
      361 caataccagc acaatgccga gcgtggtgat gatcgacaac gccccgcaca agagcagcac
      421 ggcgtggacc gcctgctctt gaacgtgctt ggtgccggtt cgcgcgatca tattcgctct
      481 tgcccgatgc gatgcatcac cggctcgcgt tacttggtct caaccgccag cagcgactcg
      541 atcgtcaccc cgacctgcga acccttcccg ccaaacaccg acccgacggc ccgcgcctcc
      601 acccgcttca tggccagctc ataggctttg tccggcaacg ggatgtaccc cacctcattg
      661 accagttccc gcccggccgt gaggtagaac ttcacgaagg ccgcgatctc cggccgatcc
      721 agcgccttct tgttcacgta gatgaagatc ggccgagaca gcggctcgta cgtggcgttc
      781 ttgatggtct ccgctgtggg caggatcgga cccgcgccgt tcttgggatt ttcatcgtcg
      841 atcgcgacca acttgagacg ccctttgttg ctgtcgtaat acgacacgcc gaaaaacccc
      901 agcgcgagcg ggtcaccggc gatgccctgg accagcacgt tgtcatcctc gctcgcggtg
      961 tagtccccgc ggctggcgtg ttccttgccg accaccgcct cggtaaagta atcgtacgtg
     1021 ccggagtcca cgccggggcc gaagaggcgg atctccttgt ccggccaacc cgcgcgaatc
     1081 tgactccagc gcgtgatctt gccctgcgca gggggctccc acagtttctt gagttcagcg
     1141 accgtcatgc tggccgccca cgtgttcttg ggattcacca tgacggccag gccgtcgaac
     1201 gccaccggca gttcgatgta ttcgacctta ttgtccgcgc attgtttgac ctcggtggcc
     1261 ttgatgggcc gcgatgcgtc cgcgagatcg gtttccccgc cgcagaactt cttgaatccg
     1321 ccgccggtgc ccgagatccc gaccgtgatc cgcaccgtgc ggttcttctt ttggaattct
     1381 tccgccacgg cctcggtaat gggatacacc gtgctggagc cgtcgacctt gatcaatgtt
     1441 gcggcatgcg cggacccggc gaacgccgtt gtccacacgc ccagcagggc cgccaagccg
     1501 attccccacc cattgcgccg actcatctcc ggcctcctat tcgcacgcgt tcgggacacg
     1561 ttggcccaat ctcggtgact ctcagaactt gacggacaac tcgatctgca accgactgac
     1621 gtctttttgc tccgccgaag aatcatcttc gacttcgctc gtcatatact ttgacttgac
     1681 ttggatcgct tgggtgggat tgtacgcagc ccacatgatg tggcccttgc ggtcggtgcc
     1741 gccgtcggtg ccgaaatccg agtcggcgat gtcggccaga gtcgcgtcgg tccccacttt
     1801 cttatagaag tacgccacct cccaggtccg aggatcgccg gccttgccga gaatcacccc
     1861 gacctgatat ccgccatctt tggtgtcgtt gcccgacgcg tcctgggtgt cggccgtgtt
     1921 cttgacggca tcccccatca ccgagaccgg cagggatgcg agtttcgtgg tcgccgccgc
     1981 cgtgacatgc accacgtcat agtcgttgac gagctccgca ttgcgcgtgg tggagcctgg
     2041 gcagagcgta cccgttgcct ggcggctgtt gccgtccgcg cagacagact ggtcgagcgt
     2101 gccgttttga gtgttcaccg cattcaggta cgtgacggcc agcgcggtct gcagcgtggt
     2161 cgtcgggttc atcttgactc cgacctgctg accgaagagc cactggtccg tcgacagact
     2221 gccggcggaa tcttcgtcga gcacgatctg ggcaaggttc acgaacacgt cgccgcctcc
     2281 gagcgcgaac gacaggtttt ccgcaagccc ttcagggtta tagtcgtcgt cccagaccag
     2341 gtcggacgaa tacactgaga aaaacgggtt gcccattcgc ccgcccgtca cggtgagcca
     2401 ccgggtgtcg tctccctgcc accgcacgaa cgcccgatcg atatacagcg gcgtcggcgc
     2461 ggacaaatca tcgaagctct tgttcgcgga cacgtgctcg ccggtcccgc tcgccaatcg
     2521 gaccccgacc acgaccgtgt cgattttcag atccgcgccc acgcgaaggc ggaaccgttg
     2581 ccgcgagcga tcatcggcgc cattgtgccg gtacaccatc tcctgccgca atcggatatc
     2641 gccgccgaac gtgatccggt ccagccacgc cgcgagtttc gcgccctgca ccgacgcggc
     2701 ttcctccttg gtcaacaccc ctttttcaac cagtaattga tccaacggct tgcgttcctc
     2761 cgctcccgcc aacgtcgcgg ccagcagcgc ccacggcgcg accaccaggc ttccgatcca
     2821 tcgtttcatg cgtctccctc cacgtccgga tgggtttcag accgtcgtga cgagctcacc
     2881 gcgcgtgtgg cgtcccggcg agatcagcaa gggaagcgcg gatcgcgcgc gagtctgtgt
     2941 cgggcgcgac cgggccacgg taacaacggc gtgtttcgcc gttgttactt ttgtgttaac
     3001 cctgtgtaaa ttgtcgactg ggaaacgggg tggacgcctc ttattcagcg tctcgtcgga
     3061 ggtcagggcc gggcgatgag ggggagaatc gaatggccag gatccggacg ccgctcagca
     3121 ccgcgatgac gaacaagagc agggtaaacg tcgcgccctc accgcctttg agccagagcg
     3181 agagaatctc ggtcaacatg accaccagga tcgtatccac gatgaacgtg accttgaccc
     3241 gtccttccgc cagatacgcc aacgtggtgc gaaacacctc cacaatggcc aacaacatga
     3301 gcacgccgat caacacgcgc tggagcacgg catccacgcc cgccgttccc aactcccgca
     3361 actccaagcc cacagacacc accccgacca acaacgtgag caggatcaat ccgagcaaca
     3421 agctcaacac cgccttgatg gccgtggtcc acacccgcgg agcatccaac ccggcgatcg
     3481 ccggcaagcg ctgccacact tcgtccaatg tcgttgaaag cgtcatactc gttctccttc
     3541 gcctctgatc tcgtcctctc tcttctctcg catcgatcat agtgggggca cgttacagca
     3601 gcgttgccgc cgcgttgaag cttcgtgaat tttcgcggcg ttcatcggcg cgcggcgtgg
     3661 actggccggc ggagcgtgtg ttacgatccg gcgcatggaa cccctgtggc tgatcgcggc
     3721 attgctggtc gcgctgattc tcgtggtcgg cgtcgcccta tttcgccgcc ggcccgaccc
     3781 gaccatcacg ttgctccaac agcaactcga cgccatgagc cggcagctca gcgaatccgc
     3841 cggcggctcg aaattgatgc acgaccgcct gggccaggtc acgcaggagg tcaacggcca
     3901 actgtccgcc atcaccaccc aggtcaacgg ccagttgggc ctgatcaccg cacaggtcaa
     3961 ccagcaactc accgccatcg gcaagcaggt ccaggaagcc accggccaaa tgggcacgcg
     4021 cctggacaac gcggcgcgcg tggtgggcga ggtccggcaa aacctgggcg agttgaccaa
     4081 ggccaccgag cgcgtgttcg acgtgggcaa ggacatcgcg agcctgcagg aaatcctgcg
     4141 cgcgcccaaa ctgcgcggcg tgttgggcga actgttcctg ggcgatctgc tcggccagat
     4201 cctcccgccc acgcactacg cgctgcagta taaattcaag agcggggaag ccgtggacgc
     4261 ggccatccac ctggggcaag gcatcgtgcc ggtggacagc aagttcccct tggagaattt
     4321 tcgccgcgta atcgcgagcg aggccgagga cgagcgcaag gccgcgaaaa aaaaattcgt
     4381 catcgacgtg aagaaacaca tcgacgccat cgcgtccaag tacatcctgc cggacgaggg
     4441 cacgttcgac ttcgccttga tgtacatccc ggccgagaac gtgtactacg aagtgatctt
     4501 gaaggacgac gcgttcggcg aggacaaagg cctgtgcgcc tatgcgctcg cgcagcgcgt
     4561 gatcccggtg tcgcccaaca gcttctacgc gtacctgcaa gccatcgtgc tcgggctcaa
     4621 aggcctgcgc attgagaaga acgcgcagga gatgatccag cacctcaaac ggttacaagg
     4681 cgactttcag cgattccggg acgacttcga cgtcttgggt aaacacctgg gcaatacgcg
     4741 gggcaaatac gaagacgcat ccaagcgtct ggagcgcttc ggcgagaaac tcttgagcgt
     4801 ggaagagtcg gccgccgcgc cgacgtccat cgaagtcgac gccgcgtctc cggcccagcg
     4861 accgctgttg taaggcctag acggaccgct ccggttcgtc ctcgttcggc acgatgaaga
     4921 tcagcgccaa atagagcagc accaccacgc cgaacgagat aaacgcggcc agcacccaca
     4981 ggatgcgcac gatcgcgggg tcgatcccca ggtacccggc catccccccg caaatgcccg
     5041 cgagtttgcg ttcgcgtcgc gagcgggtca gacgccgggg atttccggtg gggttgagga
     5101 acgacccgca gtagcggcac ttgatcgcgt cgtcctgaat ctcctccgcg cagtacgggc
     5161 aatgtttcat aatttatacc ctaccaaaag cgcgcgcgca ttccaatgcc ggggtccgtc
     5221 ttcctaaggc gacgcccgcg ccaacttgcc ggaccaggga aacgcggtat atagttccgg
     5281 tgttcgcgat cccaccgcac catcacccgg aggacatccc catgaacgaa tgcagccgat
     5341 ggatcgccgg aatcctgctc tgtctcgtca taggcggcgg ggtcgggagc gcgcatgccg
     5401 acgaggccga acgcgacctt gcctggatgg ttgggccggc cagggtgccg ttggccgacc
     5461 aagcggtcct aaccttgccg gagggttatc gcgcgatcgg ccccgacgat acccaatacg
     5521 tgctcaaaga gatggggaac tacccgtctg gcactgaact cgcgttgatc gcgccgagcg
     5581 cggatagccc ggattcatgg ttcgtggtgg tccgctatgt ggacgcgggg tatatccgag
     5641 acgacgaggc aacggattgg gacgcggacg ggctgctgac ttccatcaga gagggcacgg
     5701 acgaggataa tcggcagcgc aaagacgcgg gacaggcggc gttgctcatt cgcgggtggg
     5761 aacaaaaacc cgcatacgac aaggcgagca acaaggtggt gtgggcgatc tcccacacgg
     5821 tcgagggcga agagggcgtc ggggtcaact acaacacctt ggcgctcgga cgccacggct
     5881 atatgagcat gaacatggtc ggcgacttga acgagcttgc ggcgatgaaa ccgaaggcgg
     5941 aactgctgtt gaccgcgatg agctttgtcg acggcaaacg gtacgccgac tttaacggcg
     6001 ccacggacaa agtggcggcc gtgggcctca ccgcgctcgt cgcgggagtc gcggcaaaga
     6061 ccgggctgct ggccaaactc tgggcgttcc tcctcccggt cgttctggtc gcgaaaaaat
     6121 tcatcgtgct gctcgtcgtg gcgatcatcg cgttgggccg tagcctatgg aaacggatgc
     6181 gggcgaacgc gggaccgccg accgcgtcgc ccgccccgtg aaaaccatcc tgctcctgct
     6241 cggcgccgcc aaactcggca aagtcgccct gaccggcgga accatgctgc tgtccatggt
     6301 cacgtactcg ttcgtgttcg gctggcggta cgcggtggga ttcgtgctcc tcatcttttt
     6361 ccacgagagc ggacattacg tggcggccaa acaacgcggg ctcaacgtcg gggccccgac
     6421 cttcatcccg tttgtgggcg cgtggatcca attgaaagac cagccccacg atgtggagac
     6481 cgaggcttac gtcgggatcg cggggccagt cgccggaacc atcgcggcgc tcgcctgcta
     6541 ctacgcgtcc gaggcgctgg gaaaccgcct cctgctggcg ttggcgtacg cgggcttcat
     6601 gatcaacatc tttaacctca tccccctctc gccgctcgac ggcggccgca tcaccgccgt
     6661 gctctcgccg agggtctggt ggatcggcgc gccgattctc gtcggcctgt tcatggtccg
     6721 accgagtccc atgctgattc tggtcgcgat cctggccgcg ccgcacctgc tggccaccat
     6781 gggccgcgga accggcccga tgccggcccg gtactacgcc gtctcccccc gaacccgact
     6841 cacctacggc gcgtattatc tcgggctcgc cgccttcctc ggcgtcatga cctacgaaac
     6901 gcaccacatg ctgggcgcgt aggccgccgt gacgtggcac tcttcatgct cgccaacaca
     6961 cccacggtga gcggaagccc ggatgattga acggcgaccc atgttcggcg tgccgaccct
     7021 gagcatcgcg ctggcgctct tcgcggcact caccgttccg gccaccacga ccgcggcgga
     7081 cgttttcacc tggaccgatg agaacggcac gatccatttc tcggattccg tgagcgacgt
     7141 gcccgccaaa taccgcaagt ccatcaagca aaaaaagttc ggtccggaca cggactcccc
     7201 gtcagccgcg cccaaaaccc cggcgcaacc gcccgatcaa gccgccgcgc cgtctccgga
     7261 acccgccccg aaagacccga gcgcgccggc ccgcttcgag gtgccgtaca cgccgtacga
     7321 gggatcggcc cgccggttga tcgtgtccgt gcgcttcaat aatcgcgtca ccgcgcccat
     7381 gattttggat accggcgctc cgtcgtcgct catttccatg gacatcgccg aagagttggg
     7441 cttgctcgac aaggagcacg gcagactgct ggtcaacatc gggggcatcg gcggcagcgc
     7501 ggtggccgtg aaaaccatcc tggaccgggt gcagatcggg gacgcgaaga tcgatttcgt
     7561 gcccgcggag atcacgtcgt cgatttcgga caagttcgag ggcctgatcg gcatggactt
     7621 catggccaac tactcgacgc agatcgactc caagaaacgc gtggtggtct tcgaacagat
     7681 cgacgacgac gcgagccgcc cgggcggata cgacgagacg tggtggcgca atacgtttcg
     7741 ggagttcgcc gactcgtaca atcggtggaa ggactacctc cagcgattgg ataaggaaat
     7801 cgagcagtcg ccgatcgtga atcgcggcat gcccacggac ctgtcgagac gccgggactt
     7861 cgcgtactgg cagacccagg aagccgaaaa actgatggac cgcctccacc gcttcgcccg
     7921 cgaaaacgcg gtccccatgc actggcgaac ctactgacgt ccggtcagag caagactcct
     7981 acggacagac ccccgcgctc ataccagcgt cgccccccgt tccagcagga gttcacgaag
     8041 cacggatctg tgacagcgcg attcgtcctc gcagtagcag cccaccgaga actgcgcgcc
     8101 atgtgacagg gccgccagca gatcaagaac ccggctgttt tcaggcttct tcatctcggc
     8161 gcggtagcgc tttacgaagc cggcccactg gcgctcggtc ttggcattga gcgccagcgt
     8221 cacggtctcc tgcgtcggcg ccacgttcga cagccagacg tcgtaccagt tctccgacga
     8281 gaatttcgat ttcggcactc cgcgcggcgg gcgcctgacc gtgccgatcc gaagcccctc
     8341 gttcggggcg cgcggggtac cgagacgcac gatgcggatg gccatgactc ccccaacgtt
     8401 gatgccgact gcaaacatcc atgggaatgt tactacgtta tgaagtttct cgacagacct
     8461 catggatggg gtgccgacgg aattaattcg ttccttagaa gcgtcccctg agttccctac
     8521 tactcgcgat tcaccatggc cgattgttca cgtatatgac aatgccaatg agtacaatcc
     8581 acatgatcgc ggcgatactg ccgttccgta cttcataggt atcggacact tctgtttcaa
     8641 ggctggtccg gcatcctttc accagccagc agaacactcc gccggttcca taaaacaaac
     8701 tcgtcaggag ttcgaaaagc gtttctgcca tgcgttgttt aatgattaag ttcaaccgaa
     8761 gtttgctgca aggacgcgtc aggctgcggt tgtcgttcga acttcccaaa aacctcaccg
     8821 atttgatacg ggaagtaaat cattccgacg agtgccgccc ctgctaatac cacgctaagg
     8881 atcaacaaca gacgtcctcg tcgtcttgcc gcgtcgacct ctaggatttc cgtatctcga
     8941 actactcgtg ttcccggagg aggacattgt ccgctggcca ggatttgctt ggcaacacga
     9001 tgcatgtagt acgccatacc gagtaccggc gcgaaaagaa tagcgatcat gaattgaaaa
     9061 ctacgcgtcg ctacccatag ttcctgcttc tttaagtact cctcaaacat tgggaagagc
     9121 cactggacag tgccgaacgt aaggaccgca aacgcaatat gcagcagcaa gatctgtcgc
     9181 cgcaaacggg ggtccgcttt caagatcgcc acaagtccgg ccctcacagg attctgagat
     9241 ttctatcggt cacgattaac cgccttcgtt cgccacggtt ctctatatat gcggactccg
     9301 ccacccagtc aacttgacaa ttcaacaaga tagatctgtc ccattgctgt tatcggagcg
     9361 ctctcgtcag cgtatgctag acttatcggc catggcttct cagaaacgct ccgtggccag
     9421 gcgcatcgga cgagcgtggc tcgccgcgtt catgctgggt cttgtattga ccggcccccc
     9481 cgaggctttg gcgatctttc agtatacgga cgacgcgggt acggtgcact tcacggacga
     9541 cccggggcag attccgga
//
LOCUS       DBNGTT010000361         1512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_597648, whole genome shotgun sequence.
ACCESSION   DBNGTT010000361 DBNGTT010000000
VERSION     DBNGTT010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_597648"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1512
                     /locus_tag="AC16BC_08150"
     CDS             24..>1512
                     /locus_tag="AC16BC_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241632.1"
                     /translation="MTRFSAADPPPQAWHPPAPWWIWVLLVGVTIAVYANSVPNGFHY
                     DDVFTILRNPAVQEVERLPRHFWSATIGNQEGTPSYRPLLMLTYGLNYWWGGTYPAGY
                     HVVNIGLHVMASVLVVLLLWSLSGHAVAAVLCGFVFALHPIQTEAVNYITARSSVLYA
                     VAGLAAVVAFTRYRATGRVEALAGSVLAYVAALLAKEAAVVVPVLLIGYDVIVRRFAW
                     RDLARWGPPHALFVAVTVAYLALRRLMMGEEITSGYQGYHADVFTAGVTFAAMVAQTL
                     SDQLVPVNLTVSHSFAPIRELTARAVGALLVVAAVAAVGIVACRRAGVLAFAVTWFVV
                     ALLPLAALPLITALALYQENRGYLSAVAVAWVGGVLLAWCWESDRARQRGVTVFRRGM
                     VLLVLGAMAVAVVVRNPVWRDDVSLWLDALSKAPGNQAAYVNLGAAYQARGDLQAAAD
                     VYQRAVERFPRNAILHNNLGALYRASGDFRRAAEEFRAAIQVNPGF"
BASE COUNT          190 a          471 c          536 g          315 t
ORIGIN      
        1 aacacggggc tctccgcgcc ctgatgacga gattttccgc ggccgatcct cccccccagg
       61 cctggcaccc gccggcgccc tggtggatat gggtgctgct ggtcggcgtc acgatcgccg
      121 tgtacgcgaa ttccgtaccc aacggctttc attacgacga cgtgttcact attctccgga
      181 atcccgcggt gcaggaggtg gaacggctgc cccggcattt ctggtcggcg accatcggca
      241 accaggaggg cacgccgtcg tatcggcccc tgctgatgct gacctatggc ctgaattatt
      301 ggtggggcgg aacgtacccc gcgggttatc acgtggtcaa catcggcctc cacgtgatgg
      361 cctcagtcct tgtcgtgctc ctgctctgga gcctctcggg ccacgccgtg gccgcggtgc
      421 tctgcgggtt cgtgtttgcg ctccatccga tccagacgga agcggtcaac tacatcacgg
      481 cccgctcgtc cgtcttgtac gctgtggcgg gactcgccgc ggtggttgcg tttacgcggt
      541 accgcgccac cggacgggtg gaggccctgg cgggatcggt tctcgcgtat gtcgccgcgc
      601 tgctggccaa ggaagccgcg gtggtggtgc cggtcctgct gatcggctat gacgtgatcg
      661 tgcggcgttt cgcgtggagg gatctcgcgc gctgggggcc gccgcacgcg ctgttcgtgg
      721 ctgtgaccgt ggcctacctg gcgctccgcc gcctcatgat gggggaggag atcacgtccg
      781 ggtatcaggg ataccatgct gatgtattca cggcgggagt gacgtttgcc gccatggtgg
      841 cccaaaccct gagcgatcaa ctcgtgccgg tcaatttgac cgtctctcac tcgttcgcgc
      901 ccatccgcga actcacggcc cgcgccgttg gggcgctgtt ggtggtggcg gccgttgcgg
      961 cggtggggat cgtcgcctgc cgtcgagccg gggtgctcgc ctttgccgtg acctggtttg
     1021 tcgtcgcgtt gttgcccttg gcggcgctgc cgctcattac ggcgctcgcg ttgtaccagg
     1081 aaaaccgggg atatctgtcc gccgtagccg tcgcatgggt gggcggcgtg ctgctggcgt
     1141 ggtgttggga gtccgaccgg gctcgtcaac ggggcgtgac ggtgttccgc cgcgggatgg
     1201 tgctgctcgt gcttggggcg atggccgtcg cggtcgttgt ccgaaacccg gtgtggcgcg
     1261 atgacgtctc gctgtggctc gacgccttga gcaaggcccc gggtaatcaa gcggcgtacg
     1321 tgaacctggg cgccgcgtat caggcccggg gggatcttca ggccgcggcg gatgtctatc
     1381 agcgggccgt ggagcgattt ccccggaacg cgatcctgca caacaacctt ggggcgctct
     1441 accgagcgag cggcgatttc cggcgggcgg cggaggagtt tcgtgccgcg atccaggtca
     1501 accccggttt tg
//
LOCUS       DBNGTT010000362         2359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3152738, whole genome shotgun sequence.
ACCESSION   DBNGTT010000362 DBNGTT010000000
VERSION     DBNGTT010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3152738"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1644)
                     /gene="bcsG"
                     /locus_tag="AC16BC_08155"
     CDS             complement(115..1644)
                     /gene="bcsG"
                     /locus_tag="AC16BC_08155"
                     /inference="COORDINATES: protein motif:HMM:NF023088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HIR3241633.1"
                     /translation="MGLWASYFFAKGALYFAGYIPLSVGWNLLFAAFLAIPTPERIAQ
                     RRAVVVARAVLHILIALALLWHDSWLPSWHEAVGFVAMGGLPTPEFVGEFLAGAINPW
                     IVGGLAILLVATAAAHRYMRLTPVVAVLLLTVGAVEFTEPKEAMDRVVHAFLTDEARQ
                     VTSFPSHDNAAPACDIVFLHVCSLSWDDLRDVGLDDSPFFRQFDYLLTDFNSVTSHST
                     IAALRLLRSTCGQTTQEALYRRGHPECYALDALRGAGYQTWFALNHSGEYMGFARDVQ
                     AWGHADAPLEIDDLPVSQYDFSGDPIRSDSAVLERWLSERERSGARRAALYYNTISLH
                     IGGRRAGEAVAAGTNEDEYRRDLRILFEEFDRFFARLASSGRDTVVMFVPEHGAALRG
                     TKLQPAGLREIPIRRITNVPVAVKLIGRRPPTGHRPQRVIATPTSYQSLAAFVNEFLT
                     RSPFEEQAWAEGPPAVRVPEIAFVAENEGVRVIQAGTEFFMKQGGAKDQWIKLPSEIA
                     M"
     gene            complement(1693..>2359)
                     /locus_tag="AC16BC_08160"
     CDS             complement(1693..>2359)
                     /locus_tag="AC16BC_08160"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3241634.1"
                     /translation="AVDLSETGVRHVHNQPRSLPESVEVRLADAVRDQPGVKGRVIRN
                     DVLDRTSAVVGIEFRDLSERQLRDVILQMYSDPSMWDKRPEIDTSAWRSFFLLGTSLV
                     HAFGKDIESLRRRDPRSPLEIPCEVISRDVVLSGATENISQAGLLIRLREGSRTVPDV
                     CSVRLIPGSDVLTFQGRIVWKAPKGTPVRAGVRLDERVSPFLISWMEFAKASRAPMGG
                     PGR"
BASE COUNT          447 a          822 c          730 g          360 t
ORIGIN      
        1 ggggatgtca ggactccggc cacgcagatt cccccgaaga ggcccctgaa ccggctccac
       61 aagtcactcg gtcgtctcac gttcatctcc tttcaggcca atacaacgtc ccgctcacat
      121 ggcgatctcg gaggggagtt tgatccattg atctttcgcc cctccctgct tcatgaaaaa
      181 ctcggtgccg gcctggatga cccgcacgcc ttcgttctcc gcgacgaacg cgatttccgg
      241 cacacggacc gcgggcgggc cctcggccca cgcctgttcc tcgaacggac tgcgggtcag
      301 gaactcgttc acgaacgccg ccaacgactg atagctggtg ggcgtggcga tcacccgttg
      361 cggccgatgg ccggtcggcg ggcgacggcc gatgagtttg acggcgaccg gcacgttggt
      421 aatgcgacga atcgggattt cccgcaaacc cgcgggctgg agcttggtcc cgcgaagcgc
      481 cgcgccgtgt tccggaacga acatgacgac ggtatcgcgc cccgacgacg ccaggcgcgc
      541 aaagaagcgg tcgaactcct cgaacaggat gcggagatcc cgccggtact cgtcttcatt
      601 ggtgccggcc gcaaccgcct ctcccgcccg ccggcccccg atatggagcg aaatggtgtt
      661 gtaatacagc gcggcgcggc gagcccctga ccgctcccgc tccgacaacc atcgttccag
      721 caccgcggag tcggatcgga tcgggtcccc ggaaaagtcg tactggctca ccggaaggtc
      781 atcgatttcc agcggcgcgt ccgcgtgccc ccacgcttgg acatcgcgcg caaaccccat
      841 gtattcgccg ctgtggttca gcgcgaacca cgtctgatac ccggcgcccc ggagcgcgtc
      901 gagcgcgtaa cattccggat ggcccctccg atagagcgcc tcttgggtcg tttgcccgca
      961 cgtggaacgg agcaaccgaa gcgcggcaat ggtgctatgg gacgtcaccg agttgaaatc
     1021 cgtcaacagg tagtcgaact ggcggaaaaa cggcgagtca tcgaggccca cgtcgcgaag
     1081 atcgtcccac gacagggaac acacgtggag aaacacgatg tcgcacgcgg gcgccgcgtt
     1141 gtcgtgactc ggaaaactcg tcacctgccg tgcctcgtcg gtcagaaacg cgtgcaccac
     1201 gcgatccatg gcttccttgg gttcggtaaa ttccaccgcg ccgaccgtca acagcaggac
     1261 cgccaccacc ggcgtcaggc gcatatagcg atgggctgcg gcggtcgcga cgaggagaat
     1321 cgccagcccg cccacgatcc acggattgat cgcgcccgcg agaaattcgc ccacgaattc
     1381 cggcgtcgga agcccgccca tggccacgaa gccgacggcc tcatgccacg acggaagcca
     1441 cgagtcgtgc cacaacagcg ccagggcgat caggatgtgg aggacggcgc gcgcgacgac
     1501 caccgcgcgc cgttgcgcga tccgctccgg cgtggggatc gccaggaacg ccgcgaacag
     1561 caggttccag ccgacgctca gcgggatata ccccgcgaag tagagcgccc ccttcgcgaa
     1621 gaaatacgac gcccacaacc ccatgtgcgc gctcgctgcg ctacgtcccg ccgcgtgaga
     1681 cccgccgacg gctcaccgcc ccgggcctcc catgggagcg cgggacgctt tggcgaattc
     1741 catccagctg atgaggaacg gactcacgcg ctcgtcgagc cgcaccccgg cccggaccgg
     1801 cgtccctttg ggggctttcc acacgatccg gccctgaaac gtcaacacgt ccgacccggg
     1861 gatcaaccgc accgaacaca cgtcgggaac cgtgcggctc ccctcgcgaa gccggatgag
     1921 cagcccggcc tggctgatat tttccgtggc gccggagagc accacgtcgc gcgaaatgac
     1981 ttcgcagggg atctccaacg ggctccgggg gtcgcggcga cgcaaggact cgatgtcttt
     2041 gccgaacgcg tggacgagcg aagtgcccag caagaaaaac gaccgccacg ccgaggtgtc
     2101 gatctcgggc cgcttgtccc acatcgaggg atccgaatac atctgaagga tcacgtcccg
     2161 caactgccgt tccgacagat cccggaactc gatgcccacg accgcgctgg tccggtccaa
     2221 gacgtcgttg cggatcaccc gccccttgac gcccggctgg tcgcggaccg cgtcggccag
     2281 gcgaacttcg accgattccg ggagcgatcg cggctggttg tgcacgtgac ggacgcccgt
     2341 ttcgctcaga tcaacggcc
//
LOCUS       DBNGTT010000363         3733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2513562, whole genome shotgun sequence.
ACCESSION   DBNGTT010000363 DBNGTT010000000
VERSION     DBNGTT010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2513562"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..888)
                     /locus_tag="AC16BC_08165"
     CDS             complement(196..888)
                     /locus_tag="AC16BC_08165"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3241635.1"
                     /translation="MVKHPRDPVRTPARAIEPVHPRIFDYLAGLHQRHDEPVLLEMEA
                     EARERGFPIVGRVVGVFIEVMALSVGARTIFEFGSGYGYSAYWFSRAVGPEGSVICTD
                     GKADNARKARTYLERVGRWPRIDFRVGWAQDAFRATEGRFDVIYNDVDKDAYPEVWRL
                     ARERVRPGGLYIADNTLWSGRVVQDRVVDDVAPGWTEAIIEHNRLIAHDPDYDCFLNP
                     IRDGVIVARRKT"
     gene            960..1502
                     /locus_tag="AC16BC_08170"
     CDS             960..1502
                     /locus_tag="AC16BC_08170"
                     /inference="COORDINATES: protein motif:HMM:NF017774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR3241636.1"
                     /translation="MEIIIDGYNVIGSRGGLYGDVAAKRDAFIAELARYARIRGHAIT
                     VVFDGFPPGVATAAGGASGVPRNPPGVRVRFSEHERADDVVIRLATRLREQATIVSSD
                     REVRAACEAQRAVVLGAQAFDARLRDALTGEGGLDHAPEAADKDRDGDEDARGGRSKR
                     GNPRRSSKAQRKSQQRLGKL"
     gene            complement(1528..3177)
                     /locus_tag="AC16BC_08175"
     CDS             complement(1528..3177)
                     /locus_tag="AC16BC_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012316775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241637.1"
                     /translation="MTSVTDSVRTLPGRRPIDGWRLGALAIAAVVVFPLAVVLSSVFA
                     PADEVWRHLADTVLVDLLRNTFWLVAGVAVGTAVLGVGLAWITAVCEFPGRRVFDWAL
                     MLPLAIPAYVTAFVAVGLFDYTGPIQTLMRDVLPAGWARLPPIRSQGGVILVMSLALY
                     PYVYLLARQAFLTQGKRAMEAAQALGHGPWRGFFHLAVPMARPWIVGGLALVVMETLA
                     DFGTVAVFNYDTFTTAIYKAWFGLFSLTAAAQLASILIVIVFALLLIERRWHAGGRYT
                     TAGKQDTTRQRIRLGNGHAWLAFGACAAVLSLAFGVPVIQLLVWGVRRLATDVDARAI
                     GFLWHSTFLGAMAAALTCACALVLAYAQRRGDGLIRIATRVSTLGYALPGSVLAVGTF
                     IPLAWLDRQVAAGFSLFQREVGLVILGTPLAMLLAYLARFLAAGFGPIESAMHRVTPN
                     VEEAARGLGVSGRRLVRRIHLPIIRSGVLTAATLVFVDVMKEMPATLMTRPFGWDTLS
                     VRIFELTSEGQWEQAAIPSLALVLAGLLPVMLLTRHEGDTV"
     gene            complement(3217..>3733)
                     /locus_tag="AC16BC_08180"
     CDS             complement(3217..>3733)
                     /locus_tag="AC16BC_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3241638.1"
                     /translation="VYNQSLVATMIATLGEAKTEEIVRSWVGNLATDVFADDTTMLEA
                     IAAGQCDVGITNTYYFGRLKAKHPDIPLALFWPNQHDRGVHVNVSGAGVTRYSKHPEA
                     AVRLLEWLSSPRAQKLFTDMDFEYPANATVSPSAAVAAWGAFKSDPLNVAKAGELQRA
                     AVMLMDRAGYR"
BASE COUNT          655 a         1228 c         1296 g          554 t
ORIGIN      
        1 agagcctgtc catgagcggt catctgcggc gttgtcacgt cggcttcgca tcctcacgta
       61 cgacccagta cgctgcggtg cgaagcctcg ttccgccttg catctgacgc gctcctgaac
      121 aggctccgct tcgcggttag aaagtctgtg gcctacacaa ttcgttcgtg ttaaggcctg
      181 tcaaagcacc tgcgctcacg ttttccgtcg ggccacgatc acgccgtcgc gaatcgggtt
      241 gagaaaacag tcgtagtccg gatcgtgcgc gatcaggcgg ttgtgctcga tgatcgcttc
      301 ggtccagccc ggcgccacat cgtccaccac gcgatcctgg acgacccggc cggaccagag
      361 cgtgttgtcc gcgatataga ggccgcccgg acggacgcgc tcgcgcgcca ggcgccagac
      421 ctcggggtag gcgtctttgt ccacgtcgtt gtagatcacg tcgaaccggc cctcggtggc
      481 tcgaaacgcg tcctgcgccc agcccacgcg aaagtcgatg cgcggccagc ggcccacgcg
      541 ttccaggtac gtccgggctt tgcgcgcgtt gtccgccttg ccgtcggtgc agatcacgga
      601 gccctcgggg cccaccgcac gggaaaacca ataggcggag tagccgtacc cgctgccgaa
      661 ctcgaagatc gtccgggcgc ccacgctgag cgccatcacc tcgatgaata cgccgaccac
      721 gcggcccacg atgggaaacc ctcgttcgcg ggcctcggcc tccatctcca gcagcacggg
      781 ctcgtcgtgg cgttgatgaa ggccggcgag atagtcgaag attctggggt gcacgggttc
      841 gatggcgcgg gccggggtgc ggacgggatc acgcggatgt ttgaccatgg gtacctccgt
      901 gacagagaca gtgccgcttc tgtaccatga ggcatcgttg ctgatcaacc acggatcgca
      961 tggaaatcat catcgacggg tacaacgtca tcggcagccg aggcgggttg tacggggacg
     1021 tggcggccaa acgcgacgcc ttcatcgcgg agttggcgcg gtacgccagg atcagaggcc
     1081 acgcgatcac cgtggtgttc gacggcttcc cgccgggcgt cgcgaccgcg gcgggcggag
     1141 cgtcaggcgt cccgcggaat ccccccggcg tgcgcgtgag gttctcggag cacgagcggg
     1201 cggacgatgt ggtgatcagg ctggcgacgc ggctccgcga gcaggcgacg atcgtctcct
     1261 cggaccgcga agtccgcgcc gcgtgcgagg cgcagcgcgc cgtggtgttg ggggcgcagg
     1321 cgtttgacgc gaggttacgc gacgcgttga cgggcgaggg gggactcgat cacgccccgg
     1381 aggccgcgga caaagatcgc gatggcgacg aagacgcgcg cgggggacgc tccaagcgcg
     1441 gcaacccgcg ccggtcgtcg aaagcccagc gcaaatcgca acagcgcctg ggaaaactct
     1501 gagcatcggg cgagccggtc ggctcgctca gacggtgtcg ccttcgtgcc tggtgagcag
     1561 catgaccggg agcaggccgg ccagcaccag cgccagggag ggaatcgcgg cctgttccca
     1621 ctggccttcc gacgtcaatt cgaaaatccg cacggacaac gtgtcccagc cgaacgggcg
     1681 ggtcatcagc gtggcgggca tttccttcat cacgtccacg aacaccaggg tcgccgcggt
     1741 caacacgccg ctgcggataa tggggagatg gatccgccgc acgagtcggc gtccgctcac
     1801 gcccagcccg cgcgcggctt cctccacgtt gggcgtgacg cggtgcatgg cgctctcgat
     1861 gggcccgaat cccgcggcga gaaatctggc caggtacgcc aggagcatcg cgagcggggt
     1921 gccgaggatc acgagcccca cctcgcgttg aaacagggag aagccggccg cgacctgccg
     1981 gtcgagccag gcgagcggga tgaatgtgcc cacggccagg accgatccgg gcagggcgta
     2041 gccgagcgtg gacacgcgcg tggcgatccg gatcaatccg tcgccgcgtc gctgggcata
     2101 ggccaggacc aaggcgcacg cgcacgtcag ggccgccgcc atcgcgccca aaaacgtcga
     2161 gtgccagaga aagccgatgg cgcgggcgtc gacgtccgtg gccagtcggc gcacgcccca
     2221 gaccaggagt tggatgaccg gaacgccgaa tgccaaggag agcaccgcgg cgcaggcgcc
     2281 gaacgcgagc cacgcgtggc cgttgccgag cctgatccgc tggcgcgtgg tgtcctgttt
     2341 tcccgcggtc gtgtagcggc ccccggcgtg ccatcgccgt tcgatgagga gcagcgcgaa
     2401 gacgatgacg atcaagatcg aggccagttg cgccgcggcg gtcaacgaaa acaggccgaa
     2461 ccacgccttg tagatcgccg tggtgaacgt gtcgtagttg aacaccgcga ccgtcccgaa
     2521 atcggccagg gtttccatca cgaccaacgc gaggccaccg acgatccacg gccgcgccat
     2581 ggggacggcg aggtgaaaga agccgcgcca cggcccgtgg cccagggcct gcgccgcctc
     2641 catggcccgt ttgccttgcg tgaggaacgc ctggcgggcc agcaggtaga cgtaggggta
     2701 cagggcgagg ctcatcacca gaatcactcc gccttgcgat cggatcgggg gaaggcgcgc
     2761 ccacccggcc ggtaacacgt cgcgcatgag agtctggatc gggcccgtgt aatcgaacaa
     2821 tcccaccgcg acgaacgcgg tcacgtacgc ggggatcgcc aggggcaaca tcagcgccca
     2881 atcgaatacg cggcggcccg ggaactcgca gacggccgtg atccaggcca acccgactcc
     2941 cagcaccgcg gtaccgaccg cgactccggc gacgagccag aacgtgttgc ggagcagatc
     3001 gacgagtacg gtgtccgcga ggtgacgcca gacctcgtcg gccggggcga agacggacga
     3061 gaggacgacg gcgagcggga acaccacgac cgcggcgatg gccaacgcgc ccaggcgcca
     3121 gccgtcgatc gggcgccttc ccgggagtgt cctcacgctg tcggtcacgc tcgtcatggc
     3181 ggcccgtccg ggcacctgac ccggctcacg ccggcgtcag cggtagcccg cgcgatccat
     3241 cagcatgacc gccgcgcgct gcagttcacc ggccttggcg acgttcagcg gatcggattt
     3301 gaacgcgccc cacgcggcta cggcggccga cgggctcacg gtggcgttcg ccggatactc
     3361 gaagtccatg tcggtgaaga gcttctgcgc gcgcggcgac gagagccatt ccagcaaccg
     3421 gaccgcggcc tcggggtgtt tgctgtaccg ggtgacgccg gcgccggaca cgttgacgtg
     3481 cacgccgcgg tcgtgctggt tgggccagaa gagcgccagg ggaatgtcgg ggtgtttggc
     3541 cttgagtcgg ccgaaataat acgtgttcgt gatcccgacg tcgcactggc cggcggcgat
     3601 cgcctccagc atcgtggtgt cgtcggcgaa cacgtcggtg gcgaggttgc cgacccacga
     3661 tcgcacgatc tcctcggttt tggcttcgcc gagggtggcg atcatcgtcg ccaccagcga
     3721 ctggttatag act
//
LOCUS       DBNGTT010000364         1665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3407822, whole genome shotgun sequence.
ACCESSION   DBNGTT010000364 DBNGTT010000000
VERSION     DBNGTT010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3407822"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="AC16BC_08185"
     CDS             complement(<1..403)
                     /locus_tag="AC16BC_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3241639.1"
                     /translation="MPKTANRVVASSTQIVFRVPGGTRCGFRRPSTQQWDFFSGLLMF
                     LQQLINGLTLGAVYALVALGYTMVYGILELINFAHGEIYMLGAYIGILSLGVMTVWGW
                     TQTAPLLSIVAAVLVAAVYCGAVGATMERVAY"
     gene            complement(557..>1665)
                     /locus_tag="AC16BC_08190"
     CDS             complement(557..>1665)
                     /locus_tag="AC16BC_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3241640.1"
                     /translation="LTILALLFGLLMASCSSKASNEIKIGAAGPMTGDQSKMGTDLRH
                     GVELAVEEWNAKGGVLGKKIVLQVEDDQHDPKQAVAVANKFVNSGVVGVIGHWNSSAS
                     IPASGVYHQAKVVMITPASTNPQLTEQGYPTIFRVCGRDDQQGPVAARFVRERFPTGA
                     VAILHDKTTYGQGLAEQFQHALGPDAKIVFSSGVTQGDKDFRAVLTTVKSAKPDVIFF
                     GGIYPEAGLLVRQARELGLTAPFISGDGTYDQKFLEIAGPAAEGTFLTFGPDPSGIPQ
                     AQAFLTKYHERFGPHGPYSIYAYDAANILLSAIAQAKTADGTRVADAIRAMTHPGAQG
                     EIRFDAKGDVLNAPYIVWTVKNGAYEQFLTPGAS"
BASE COUNT          299 a          536 c          535 g          295 t
ORIGIN      
        1 ggtaggccac gcgttccatg gtggcgccca ccgcgccgca gtacaccgcc gccaccagca
       61 ccgcggccac gatcgagagc agcggcgcgg tctgggtcca gccccagacc gtcatgaccc
      121 ccaacgacag aatgccgata tacgcgccca gcatgtagat ttcaccgtgg gcgaagttga
      181 tcaactccag gatgccgtag accatggtgt aaccgagcgc gacgagggcg tacacggcgc
      241 cgagggtgag gccattgatg agttgctgga ggaacatcag cagaccgctg aaaaagtccc
      301 actgctgcgt gctcggtcgt cggaaaccgc accgtgtccc gccagggaca cggaacacga
      361 tttgcgtcga agacgcgacc acgcggttcg ccgtcttcgg caactcctca cgtacgactc
      421 agtacgctcc gggttcctcc ttcctgcgct cttgcgaccc accgccaaga agagaaatac
      481 cggaagtccc cggagggggc atagggggca tttggagctt tttgagcggc ctgcgggagc
      541 caaaggtgtt ttcgacttac gaagcacccg gcgtcaaaaa ctgctcgtac gcgccgttct
      601 tcacggtcca cacgatgtac ggcgcgttga gcacgtcgcc tttggcgtcg aagcggatct
      661 cgccttgcgc gccgggatgc gtcatggccc ggatcgcgtc ggcgaccctc gtcccgtcgg
      721 cggtcttggc ctgggcgatc gcgctgagta ggatattggc ggcgtcatac gcgtagatcg
      781 agtacgggcc gtgcggtccg aagcgttcgt ggtacttggt gaggaaggct tgcgcctggg
      841 gaatgcccga cggatcgggt ccgaacgtga ggaacgtgcc ctcggccgcc ggccccgcga
      901 tctccagaaa cttctggtcg tacgtgccgt cgcccgagat gaacggcgcg gtcagcccca
      961 actcgcgcgc ctgtcgcacc agcagaccgg cttcagggta gatgccaccg aaaaagatca
     1021 cgtcgggctt ggcggatttg accgtggtga gcaccgcgcg gaaatccttg tcgccctgcg
     1081 tgacgccgct ggagaagacg atcttggcgt ccggcccgag ggcgtgctgg aactgctcgg
     1141 ccaacccctg gccgtaggtg gtcttgtcgt gcaggatcgc gaccgcgccg gtcgggaacc
     1201 gctcgcgcac gaagcgcgcg gccaccgggc cttgctggtc atcgcgcccg cacacgcgaa
     1261 agatcgtggg gtatccctgt tcggtcaatt gcggattggt ggacgcgggg gtgatcatga
     1321 cgaccttggc ctggtggtac acgccggagg ccggaatcga cgcgctggaa ttccaatgcc
     1381 cgatcacgcc caccacgccg gaattgacga acttgttggc cacggccacg gcttgcttgg
     1441 gatcgtgctg atcgtcctcg acttgcagga cgatcttctt gcccagcacg ccgcctttcg
     1501 cgttccactc ctccacggcc aactccacgc cgtgacgcag atccgtgccc atcttgctct
     1561 gatccccggt catgggcccg gccgcaccga tcttgatctc gttggaagcc ttcgaggagc
     1621 aggacgccat caggagcccg aataacaggg ccaggatggt caacg
//
LOCUS       DBNGTT010000365         3023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2642019, whole genome shotgun sequence.
ACCESSION   DBNGTT010000365 DBNGTT010000000
VERSION     DBNGTT010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2642019"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="AC16BC_08195"
     CDS             <1..201
                     /locus_tag="AC16BC_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3641 domain-containing protein"
                     /protein_id="HIR3241641.1"
                     /translation="ISVGWDGTLYDCDFNQMLDLPVVPEAPQHIRDFDLDRLRNRRIV
                     TGNHCYGCTAGAGSSCGGAITS"
     gene            390..1349
                     /gene="msrP"
                     /locus_tag="AC16BC_08200"
     CDS             390..1349
                     /gene="msrP"
                     /locus_tag="AC16BC_08200"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR3241642.1"
                     /translation="MLIQRAPEVKSSEITPYAAYMNRRRFLEAAGYLAVAGTAMNLIP
                     GLLSPTPARAGMKLDGVKKGAFGIDETPTPYVSVTSYNNYYEFSTDKEEPAHLAKAFK
                     PRPWSIAVEGEVKRPAAYGLEEFIKPYALEERVYRLRCVEGWSMVIPWVGFPLADLLR
                     RVEPLSKARYVEFTTLHDPAQMPGQRRSVLAWPYVEGLRLDEAMHPLAILAIGLYGEV
                     LPNQNGAPLRLVVPWKYGFKSIKSIVKIRLTDLQPRTTWAATNPREYGFYANVNPEVD
                     HPRWSQARERRVGDPLKRKTLLFNGYADQVASMYAGMDLAKNF"
     gene            1425..2078
                     /locus_tag="AC16BC_08205"
     CDS             1425..2078
                     /locus_tag="AC16BC_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR3241643.1"
                     /translation="MTPWIKPAVFGICLFPLVWLAWDGVTGHLGANPIEAVVRRTGVW
                     AINFLLISLAVTPARRLPGGNRLIAFRRMLGLFAFFYAGVHLMTYVGLDQFFAVQDIV
                     KDVIKRPFITIGMASFILLVPLAVTSTNRMIARLGGARWRMLHRLVYVIGIGGVLHYF
                     WLVKADIRSPLIYAGLLAALLGARVWIAYGSRLSASRPAPRDQGGMLGPRSAEVMSR"
     gene            2146..2853
                     /locus_tag="AC16BC_08210"
     CDS             2146..2853
                     /locus_tag="AC16BC_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3241644.1"
                     /translation="MSAPLLILIVEDDPKTSNLLKVYLEREGYATRTAADGAQAVDIA
                     QRHRPALIVLDLMIPKLDGLEVCRRIRQTSDTPVLMLTARVEEVDKLVGLSIGADDYV
                     TKPFSPREVVARVKAILRRASPKPDGTAVTILASGDLVVDEAKAKVTLKGKPVSLTAI
                     ELRLLTKLMRSPGTVFSRAQLLDALYTQDTVHVLDRTVDVHIGRLRDKIEPAPSQPCY
                     VLTVRGLGYKFRDQDRT"
BASE COUNT          516 a          953 c          990 g          564 t
ORIGIN      
        1 atctcggtgg gctgggacgg caccttgtac gattgcgact tcaatcaaat gttggacctg
       61 ccggtcgtgc ccgaggcgcc gcagcacatc cgcgatttcg acttggaccg tctgcgcaac
      121 cgccggatcg tgaccggcaa ccactgttac ggttgcaccg cgggagccgg atcttcctgc
      181 ggcggggcca tcacgtcgta aggtagtgtc ggggagcgcc tgctccccgg cattcgctcg
      241 tccctggtct gtccatcccc gcacatttgc gctccaaacc acgtcacaca atcgtcatga
      301 gtccgtggtc aggccgtgat gcggccttgg taccgtaggt cacgtcacac ggccggggct
      361 cgcgcccggc gacaccacat gagggaccca tgctgatcca gcgcgcaccc gaggtgaaat
      421 catccgagat cacgccctac gccgcgtaca tgaaccgccg ccgattcctg gaggcggccg
      481 gatatctggc cgtggccgga accgccatga acctgattcc gggacttctg agtcccacgc
      541 ctgcgcgggc cggcatgaag ctggatgggg tgaagaaggg agcattcggg attgacgaga
      601 cgccgacccc gtacgtgagt gtgacgtcct ataacaacta ttacgagttt tccacggaca
      661 aggaggagcc cgcgcacctc gccaaggcgt tcaagcccag gccgtggtca atcgcggtgg
      721 agggcgaggt gaagcgcccg gcagcgtacg gcctggagga gttcatcaag ccgtatgcgc
      781 tggaggagcg cgtctaccgg ctgcgctgcg tggaggggtg gtccatggtg atcccatggg
      841 tgggattccc attggccgac ctgctgcgcc gcgtggagcc cttgtccaag gctcggtatg
      901 tggagttcac cacgttgcac gatcccgcgc agatgccggg ccagcgacga tccgtcctcg
      961 cgtggcccta cgttgagggg ctccggctgg acgaggccat gcacccgctc gcgatcctgg
     1021 ccatcgggct ctacggcgaa gtgctgccca accagaacgg cgcgcccctc cgcctggtgg
     1081 ttccgtggaa gtacggtttc aagagcatca agtcgatcgt gaagatccgg ctcacggacc
     1141 tgcagccgcg gaccacgtgg gccgcgacca acccgcggga gtacgggttt tacgccaacg
     1201 tgaatcccga ggtggatcac ccgcggtgga gccaggcgcg ggagcgacgg gtcggggacc
     1261 cgctcaagcg caagaccttg ttgttcaacg gctacgcgga ccaggttgca tccatgtacg
     1321 cggggatgga tttggccaag aacttctaac cacgccgcgc aatactcgcg ggcggcagct
     1381 agggaggggt accaatggtc aggacattct tcaagaacgg gtggatgaca ccgtggatta
     1441 aaccagcggt attcgggatc tgtcttttcc cgttggtctg gctcgcatgg gacggcgtga
     1501 ccgggcactt gggcgcaaac cccattgaag ccgtggtgcg gcgaaccggg gtgtgggcga
     1561 tcaacttttt gctgatctcg ttggccgtca cgccggcccg tcgcctgccg ggcggaaacc
     1621 ggctcatcgc gtttcggcgg atgctgggct tgttcgcgtt tttctacgct ggcgttcatc
     1681 tgatgacgta cgtgggtctg gaccaattct ttgccgtgca ggacatcgtc aaggacgtca
     1741 tcaagcggcc gttcatcacc atcggcatgg cgagctttat cctgctggtt ccgctggccg
     1801 tgacctcgac caatcgcatg atcgcgcgcc ttgggggagc gaggtggcgc atgctgcatc
     1861 ggctggtgta cgtgatcggc atcggcgggg tccttcatta tttctggctg gtcaaggcgg
     1921 acatccggtc ccccctgatc tacgcggggc tcctggccgc gctgctcgga gcacgagtgt
     1981 ggatcgccta cgggagccgg ctctccgcga gccgtccggc gccccgtgac caaggaggaa
     2041 tgctcgggcc ccggtccgcc gaagtcatgt cccggtgaag cgggggatcg cgcgatcgcc
     2101 gctccgtgac ctgacccgac gccgatcgtg tagaatcatc gcgtcatgag cgcgcccttg
     2161 ctgatcctga tcgtcgagga cgaccccaag acctcgaacc tgctcaaggt ttatttggaa
     2221 cgggaagggt atgccacgcg cacggccgcg gacggcgcgc aggccgtgga cattgcccag
     2281 cgtcaccgcc cggccctgat cgtgctggac ctgatgattc ccaagttgga cgggctggag
     2341 gtttgccgaa ggatccgaca gacgtccgat acgccggtcc tcatgctgac ggcccgggtc
     2401 gaagaagtgg ataagctggt cggcctgtcg atcggcgcgg acgattacgt gaccaagccg
     2461 ttttccccgc gcgaggtcgt ggcgcgcgtg aaagcgattc tgcgccgcgc gtcgccgaaa
     2521 cccgatggca ccgcggtgac gatcctggct tccggcgacc tggtggtgga cgaggccaag
     2581 gccaaggtca cgctgaaggg caaacccgtg tcgctcacgg ccattgagtt gcgactcctg
     2641 accaagttga tgcgctcccc aggcaccgtg ttctcccgcg cgcagctcct ggacgcgctc
     2701 tatacgcagg acacggtgca cgtgctcgac cgcaccgtgg acgtgcacat cggacggctg
     2761 cgcgacaaga tcgaacccgc gccgtcgcag ccctgctacg tgctcaccgt gcgcgggctg
     2821 gggtacaagt ttcgcgacca ggaccggaca tgagccctgc gccgcgctcc ggtccgcgcc
     2881 gccttccatt gctctggaag ctgatgggca ttcacctgct cgtcatctcg gtggtcgtcg
     2941 ccgtcatctg gatcgcgatc cagcgcatcg cgggcaatta cttcatgcaa ttgatgcagg
     3001 actaccacat cgacccggtc atg
//
LOCUS       DBNGTT010000366         3803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_982147, whole genome shotgun sequence.
ACCESSION   DBNGTT010000366 DBNGTT010000000
VERSION     DBNGTT010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_982147"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="AC16BC_08215"
     CDS             complement(<1..243)
                     /locus_tag="AC16BC_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3241645.1"
                     /translation="MATYQYIFTMQKVTKVVPPKRKILEDISLSFYPGAKIGVLGLNG
                     SGKSTLLRIMAGVDTEFLGEAKPAKGIKIGFLSQEPQ"
     gene            complement(244..837)
                     /locus_tag="AC16BC_08220"
     CDS             complement(244..837)
                     /locus_tag="AC16BC_08220"
                     /inference="COORDINATES: protein motif:HMM:NF021613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR3241646.1"
                     /translation="MQRILFFDIETVPTEHGFTHGKPRGPEESEEQYLKRLSLSALTA
                     RVLCVGYALEPPTDAPVQILAGDEAAILKEFWGLVERADLFVGHNVLDFDLKFMVQRS
                     TIHRIKPSREIPFARFRSAPVFDTMQEWSRWGRDAASLDALAVALGLASPKDGMDGSK
                     VYPAFVVGKLEEIYRYCRGDVDTVRKVYRRLKFAEGS"
     gene            complement(979..2697)
                     /locus_tag="AC16BC_08225"
     CDS             complement(979..2697)
                     /locus_tag="AC16BC_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3241647.1"
                     /translation="MPRHWSFVVVVLGLSAASCASPARFQQTPPVRSVVNPPAAAPRP
                     NAVARPAPPAPAPNVAVPSNAPSAPETPLPIIPELSVIEEVAPDAADTDEADGDAQLQ
                     AESEAEADGGLPYDVPIVLNASVESHLDYFSTTARDRFQRWLNRSGRYLPLMTQIFRE
                     YGLPEDLVFVSLIESGFNPYAYSRSHAVGAWQFMKGTGLKYGLRIDPYIDERRDVVKS
                     THAAAAYLKDLYGMFNSWPLALASYNGGEGRVGRAMKRTNAEDFWDLRATRHLHPETR
                     NYVPKFMAATIIAKNPAKYGFTTQYDPPLAYDEVVITRPTDLRVIAKVASVTYGELKH
                     LNPELRRSVTPLGYDQYRLKLPVGTRAVFEQNFSKLPEWSKTVWMRHTVRRGETLASI
                     ARRFGTSSAVLRDMNQRSGDRIRVGATVLVPAGSQSPQYAGAPARPAVRPAASAPNTT
                     YRVRRGDTLWHIAQKFDVPLGDLRVWNGLSRRDGIQAGQRLVLHPTSDQPARVKSVDL
                     TDDSPPSKRPKRLTYRVKRGDTLWALSKNFNVPISDIRKWNGLSRTGTIRSGQRLVLY
                     VNAEQS"
     gene            complement(2859..>3803)
                     /locus_tag="AC16BC_08230"
     CDS             complement(2859..>3803)
                     /locus_tag="AC16BC_08230"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR3241648.1"
                     /translation="VLTAGFSHGVMVTDREQIKAFGVDRKKLASIGVDAFFQQFFVAG
                     FFHADPHPGNFFAMPNGTLCLHDFGMVGYLDQASRRELLSCLIAFVNKDIEAFIKHIL
                     HLAVVDDHSDVAGFEKDAAGILSEFFFSEHPPSVAWAFFRIINTASGRGIRFSGDLAL
                     FGKALVTTEGMGGKLYPEFDLNSELAPFVTSALKGYFSPTKAFEQLQTDLLDYAAAIK
                     TFPDRVTNVLNKFEKGEIGVKLDAGDLSGMKKEFDRQNDVRVLGMVLTAVGVATLALL
                     HFEGYTTLFGVHLSSVGLVVSGFLLVWFIARLRKGPGP"
BASE COUNT          685 a         1222 c         1231 g          665 t
ORIGIN      
        1 ttggggctcc tgggacagga acccgatctt gatgcccttg gcgggtttgg cctcgcccag
       61 aaattcggtg tccacgcccg ccatgatccg cagcagcgtg gacttgcccg agccgttcaa
      121 ccccagcacc ccgatcttgg ccccgggata aaacgacaac gagatgtcct caaggatctt
      181 gcgcttgggc gggaccacct tcgtgacctt ctgcatcgtg aaaatgtatt gatacgtcgc
      241 catctaactc ccttccgcga atttcaaccg tcgatacacc ttgcgcaccg tgtccacgtc
      301 gccgcggcag taccggtaga tctcctccag cttgcccacg acgaacgcgg ggtacacctt
      361 ggagccgtcc atcccgtcct tgggtgacgc cagacccagg gccactgcca gcgcgtcgag
      421 actggcggca tcccggcccc agcgcgacca ctcctgcatg gtatcgaaca ccggcgcgct
      481 gcgaaaccgc gcgaacggga tctcgcggct gggcttgatc cggtggatgg tggagcgctg
      541 caccatgaac ttgagatcaa aatccagcac attgtggccc acgaacaaat ccgcgcgctc
      601 gaccagtccc caaaactcct tgaggatcgc ggcctcgtct cccgccagaa tctgcacggg
      661 cgcgtccgtg ggcggctcca gcgcgtaccc cacgcacaac acgcgcgcgg tgagcgcgga
      721 gagcgacaac cgcttgaggt attgttcctc gctctcctcg gggccgcgcg gcttgccgtg
      781 cgtaaacccg tgctcggtgg gcacggtttc tatgtcgaaa aacagaatgc gctgcacgca
      841 aaccctccgc cggggggctg attagtatac ggggtttacc ggctcaggcg caacgcgggc
      901 gcacggagga tgggcgttga tcgccgtccc ggttcggaac gatggatcgt cccaccgcgg
      961 cacgctcgac gggatcggtt aggactgctc cgcgttgacg tagaggacga ggcgttgtcc
     1021 ggagcggatc gtaccggtcc ggctcaatcc gttccatttg cggatgtcgc tgatcgggac
     1081 gttgaagttc ttcgagagcg cccagagcgt gtcgccccgc ttgacccggt aggtgaggcg
     1141 tttggggcgc ttggatgggg gggaatcgtc ggtcaggtcc accgacttga cccgcgccgg
     1201 ttggtcggag gtggggtgga gcacgaggcg ctgtccggcc tgaatcccgt cgcgacggct
     1261 caatccgttc cagacccgga gatcacccag cggcacgtcg aacttctgcg caatatgcca
     1321 gagcgtatcg ccgcgccgca cgcgatacgt cgtgttcggg gccgacgcgg cggggcggac
     1381 cgcggggcga gcgggggcgc cggcgtactg cggtgactgt gaaccggcgg gcaccagcac
     1441 ggtcgcgccg actcggatcc ggtcgccgga gcgctgattc atgtcgcgga gcacggccga
     1501 cgacgtgccg aagcgacggg cgatcgacgc aagggtttcg ccgcgacgaa ccgtgtgccg
     1561 catccagacc gtcttgctcc attccgggag tttcgaaaaa ttctgctcga acacggctct
     1621 ggtgccgacc ggaagcttga ggcggtattg gtcgtatccg agcggagtca cgctccgtcg
     1681 cagttccggg ttcaggtgtt tgagctcgcc gtaggtgacg ctcgccactt tggcgatcac
     1741 gcgcaggtcg gtggggcggg tgatcaccac ctcgtcatag gccaacggcg ggtcgtactg
     1801 ggtggtgaac ccgtatttcg cgggattctt cgcgatgatg gtggcggcca tgaacttggg
     1861 gacgtagttg cgcgtttccg gatgcaggtg gcgcgtggcg cgaaggtccc agaaatcctc
     1921 ggcgttcgtg cgcttcatgg ctcgcccgac ccggccctcg ccgccgttgt acgaggcgag
     1981 cgccaggggc cacgaattga acatcccgta caggtccttg agatacgcgg cggccgcgtg
     2041 cgtggacttg accacgtcgc gccgctcgtc gatgtacggg tcgatgcgaa gcccgtattt
     2101 caggccggtg cccttcatga attgccacgc gcccaccgca tgcgagcgcg aatacgcgta
     2161 cgggttgaag ccgctctcga tcaacgacac gaacacgaga tcctcgggaa gcccgtactc
     2221 gcgaaagatc tgcgtcatga gggggaggta ccggcccgac cgattcagcc agcgctggaa
     2281 gcggtcgcgg gccgtggtgc tgaaatagtc gagatggctt tccacgctcg cgttgaggac
     2341 gatgggcacg tcgtacggca ggccgccgtc cgcttccgcc tcgctctcgg cctggagctg
     2401 cgcgtccccg tcggcctcgt ccgtgtcggc ggcgtccggc gccacctctt cgatcaccga
     2461 cagttccggg atgatcggca gcggcgtctc gggagcgctg ggcgcgttgc tgggaacggc
     2521 gacgtttgga gccggcgccg gtggcgccgg ccgcgcgacc gcgttgggcc ggggagccgc
     2581 ggcgggcggg ttgaccacgc ttcgaaccgg cggcgtctgc tgaaaccgag ccggcgacgc
     2641 gcaggaggcc gccgacaggc cgagaaccac gacaacgaag gaccagtgcc tgggcatgcg
     2701 ctccctcaca tccgtatgcg ggaaccgaga aggaccgctt cgccgcgatc cctcgcggca
     2761 catggcgatc ccccacctgc gtaacagact atgaaggtat ggaaaatctc accaggaagt
     2821 caatggtcgt ccgcgatgag gggcaagcgc tcgggacgct acggtcccgg tcccttgcgc
     2881 aagcgagcga tgaaccacac cagcaggaaa ccactgacca ccaaacccac gctgctcaga
     2941 tgcacgccga acaacgtggt gtagccttcg aaatgcagca aggcgagggt cgcgaccccc
     3001 accgcggtca gcaccatccc caacacccgg acatcgttct ggcggtcgaa ctcctttttc
     3061 atgcccgaca ggtcgcccgc gtccagtttc acgccgatct cgcccttctc gaacttgttc
     3121 aggacattgg tcacccggtc gggaaacgtt ttgatggccg ccgcgtaatc cagcagatcg
     3181 gtctggagct gctcgaacgc cttggtgggc gagaaatacc ctttgagcgc gctggtcacg
     3241 aacggcgcca actccgagtt cagatcgaac tccgggtaga gtttgccgcc catgccttcg
     3301 gtggtcacca gcgccttgcc gaagagcgcc aggtctcccg agaaccggat gccgcgaccg
     3361 ctcgcggtgt tgatgatccg gaaaaacgcc caggccacgc tgggcgggtg ctcggagaaa
     3421 aagaactcgc tcaagatccc ggccgcgtcc ttctcgaatc cggcgacgtc gctgtggtcg
     3481 tcgaccacgg ccagatgcag gatgtgtttg ataaaggcct cgatgtcctt gttcacgaac
     3541 gcgatcaaac agctcaagag ttcccgacgc gacgcctggt ccaggtaccc gaccatgccg
     3601 aaatcgtgca ggcagagcgt gccgttgggc atggcgaaga aattcccggg atgcgggtcc
     3661 gcgtggaaaa atcccgccac gaagaattgc tggaaaaagg cgtccacccc gatcgaggcc
     3721 agcttcttgc gatccacccc gaacgccttg atctgctcgc gatccgtgac catgaccccg
     3781 tgggagaacc cagcggtcaa cac
//
LOCUS       DBNGTT010000367         2188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_982208, whole genome shotgun sequence.
ACCESSION   DBNGTT010000367 DBNGTT010000000
VERSION     DBNGTT010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_982208"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC16BC_08235"
     CDS             <1..387
                     /locus_tag="AC16BC_08235"
                     /inference="COORDINATES: protein motif:HMM:NF015853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR3241649.1"
                     /translation="VDESDLETRTREVAKTLRCTVCQTENVWESGSPFAQTVRANIRE
                     RLQQGQSPEEIRAYFLSRYGDYILMQPPTRGVNWIVWVGPFVLLAVGGVLLYRSLARW
                     VTPAAPPPVQPLDEASRSRIERELHS"
     gene            404..1324
                     /gene="ccmI"
                     /locus_tag="AC16BC_08240"
     CDS             404..1324
                     /gene="ccmI"
                     /locus_tag="AC16BC_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR3241650.1"
                     /translation="MFLVVVGLLMAVVLALLSMPWWRRDPEPIPVGYAADQDQEQIDL
                     VIERESLIRSLKELEVEHGQGRLLDADYARLRATDERRLLQVLDRIEALSRTASAQPE
                     ISAPPVRATAAGRWLSAVVPVVLVLVASSGIYAYLQWRQARAWDAVSAQAGPSGMPDP
                     RQMVARLEERLRANPNDLEGQIMAGRSYMALDRLQDAKQAWAKVLELDARNHEAHYRL
                     GVIMINTRKFDDPELFRAALAHFDQALVNVPMEPAVNWYRGLALWYLKRYRETEEAWS
                     TAAQNLPPGSEDAEFVKGALVKLRAGQVPF"
     gene            1443..1739
                     /locus_tag="AC16BC_08245"
     CDS             1443..1739
                     /locus_tag="AC16BC_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HIR3241651.1"
                     /translation="MPELQKSRRGFAAMDPAKQRAIASRGGKAAHEKGTAHQFTAEEA
                     RLAGRKGGEIAHQRGTAHEFTSEEARVAGRKGGRSRRASGTPLPPSEPRGGQAV"
     gene            1906..>2188
                     /locus_tag="AC16BC_08250"
     CDS             1906..>2188
                     /locus_tag="AC16BC_08250"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3241652.1"
                     /translation="MARHIWIWERVAGVLLAAVTASVYVAGGFERPELMTYDWRLRAF
                     PGPPRSDIAIVGIDSPSLKALDTWPWPRSVHAALVDRLTQANARVIAFDV"
BASE COUNT          381 a          739 c          696 g          372 t
ORIGIN      
        1 gtggatgaat cggaccttga gacgcgaacg cgggaagtgg ccaagaccct gcgctgcacc
       61 gtctgtcaga ccgaaaacgt ctgggagtcc ggatcaccgt tcgcccaaac ggttcgcgcg
      121 aacatccgcg aacgcctcca acagggtcag tcgccggagg aaatccgcgc ctactttttg
      181 agccgatacg gcgactacat tttgatgcag ccgcccaccc gcggcgtcaa ttggatcgtc
      241 tgggtggggc cgttcgtcct gctggcggtc ggtggcgtcc tgctgtaccg ctcattggcg
      301 cgttgggtca cacccgccgc cccgccgccc gtgcagccac tcgacgaggc gtcccgatcg
      361 agaatcgagc gcgagctgca ttcgtgacca ggcggtatca ctgatgttcc ttgttgtcgt
      421 cggtctcctg atggccgtcg tgctggccct tctctctatg ccgtggtggc gtcgcgatcc
      481 agagcccatt ccggtcggtt acgccgcgga ccaggatcaa gaacagatcg acttggttat
      541 cgagcgggag tccttgatcc ggtcgctcaa ggaattggaa gtcgaacacg gccaggggcg
      601 actactggac gcggattacg cgcggctcag agccactgac gaacgcaggc tgttgcaggt
      661 tctggatcgg atcgaagccc tctctcgcac cgcctccgcg caacccgaaa tcagcgcgcc
      721 ccccgttcgt gcgacagccg ccggacgatg gctctcagcc gtggtgccgg ttgttctcgt
      781 gctggtcgcc tcgtcgggca tttacgcgta cctccagtgg cgccaggcgc gcgcctggga
      841 cgcggtctcg gcccaggcgg gcccctcggg gatgccggac cctcgccaaa tggtcgctcg
      901 gctcgaggaa cggctccgcg ccaaccccaa tgacctcgaa ggccagatca tggccggacg
      961 gtcgtatatg gcgcttgatc gcctgcaaga cgccaaacaa gcctgggcaa aggtcctgga
     1021 gcttgacgct cgcaatcacg aagcgcacta ccgcctcggc gtgatcatga tcaacacgcg
     1081 caagttcgac gatcccgagc tattccgggc cgcgttggcg catttcgacc aagccctggt
     1141 caacgtcccc atggaaccgg cggtgaactg gtaccgcggg ttggcgctgt ggtatctcaa
     1201 acggtatcgc gagaccgaag aggcgtggag cacggcggcg cagaacctgc cgcccggcag
     1261 cgaagacgcc gagttcgtga aaggcgcctt ggtgaaactc cgagccggac aggtcccgtt
     1321 ctagtcggct gatcccgcgc cgacttgaca cgggttcggc cgcgttcata tcatccaggc
     1381 aggtcccttc gatcacggtc tcggtgtcgg gtcgttcccc ttccacgcaa ggaggaattg
     1441 ccatgccgga gctccagaaa agtcgtcgcg gatttgccgc aatggacccc gccaagcaac
     1501 gcgcgatcgc gagtcgcggc gggaaagccg cccatgaaaa gggcacggcc catcagttta
     1561 cggcggagga agcccgcctc gcgggccgca aaggcggaga aatcgctcac cagagaggca
     1621 ccgcccacga gttcacctcc gaagaagccc gcgtcgccgg ccgcaagggc gggaggtcca
     1681 ggcgagcctc cggaacccca ctgccgccat ccgaaccacg gggcggacaa gccgtgtaac
     1741 ggatacccga cccgggtggc ggtcctcagc cgtaactgct accccaggcc gatagtacgt
     1801 ccttcgggtc gaggccccgg gatttcgcgc cgcacccgtc acttttccgc tcaccagctt
     1861 tacagcgccc ggcggcttgg tagactcggc tgcggttgcg caccgatggc tcggcacatc
     1921 tggatatggg aacgggtcgc tggcgtgctc ctcgccgccg tcacggccag cgtctacgtc
     1981 gccggcggct tcgagcgccc ggagctgatg acgtacgact ggcggctgcg cgcgttcccg
     2041 ggaccaccgc gcagcgacat tgccattgtc ggtatcgaca gcccgagtct caaggccctg
     2101 gacacctggc cctggccgcg atccgtgcac gcggcgctcg tcgaccggct cacccaggcc
     2161 aacgcccgcg tcatcgcgtt cgacgtcg
//
LOCUS       DBNGTT010000368         4871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2132661, whole genome shotgun sequence.
ACCESSION   DBNGTT010000368 DBNGTT010000000
VERSION     DBNGTT010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2132661"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="AC16BC_08255"
     CDS             complement(<1..398)
                     /locus_tag="AC16BC_08255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3241653.1"
                     /translation="MATSTAIDAGAVVEALKGHRSFSVSTHVSPEGDALGSAIALALA
                     LRSAGKTADVVIKDPVPGFLDFLPYQGVVQRHNTLPQGYDVLAVVDCGDLARTGMFES
                     APPRVKLVVNIDHHITNRGFGGLNWIVPDAT"
     gene            complement(376..792)
                     /gene="rbfA"
                     /locus_tag="AC16BC_08260"
     CDS             complement(376..792)
                     /gene="rbfA"
                     /locus_tag="AC16BC_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3241654.1"
                     /translation="MNGVGERMRAGFRRTDRVGDLMRNEIADVLLRRVKDPRVGFVTV
                     TGVKVSPDLRHATVFVSLLDEGAPAAETLAALDRAAGFVRTELGRRMRMKYTPELVFK
                     QDTTPSRAAHLEAVLDELSEARGETGDDAHGDEHRD"
     gene            complement(789..1070)
                     /locus_tag="AC16BC_08265"
     CDS             complement(789..1070)
                     /locus_tag="AC16BC_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR3241655.1"
                     /translation="MVIGVCRVELHLPDRHSLKEKRYVVKSLKDRIRKDFNVSIAEVG
                     DQDVWQRAALAAACVGTDRAYVNGVLDQLLRMVEREDGVEIVRSELEFV"
     gene            complement(1088..3514)
                     /gene="infB"
                     /locus_tag="AC16BC_08270"
     CDS             complement(1088..3514)
                     /gene="infB"
                     /locus_tag="AC16BC_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3241656.1"
                     /translation="MRVFELAKQLGKTSKELLADLAKQGTALKNHMSVLEDDMVEALM
                     KKYGAKKEAAPEAAPRRGQVLIKKRVQAEAPPAPVVEAPVAPPPPEIAAPSQPGAPAP
                     RQAPEVFRPHAPVVVPQPPMPEPIRPAAVQPAAPAAPPRGPVSTETLVKEREKEKERL
                     KKGPKKGAKRDKEAEAAAVPLQQDALVWQDFKPLHRRDERLRAQLRRAHQGGPTVEAT
                     KPRQKAVKLRAGLTVKDLADLLGVKVAQVIGQLMAMGTAASINQTVDLEAAMLVAEHF
                     GVKAEGVAERTEEEMIEAVPDEPGMRVPRPPVVTIMGHVDHGKTSLLDAIRSTKVTEQ
                     EAGGITQHIGAYTVSVHGKSVTFLDTPGHEAFTSLRARGAKVTDLVVLVVAADDGVMP
                     QTVEAINHAKAGDTPIIVALNKMDKPEAQPDRVKNQLSEHGLISEAWGGTTIFVEVSA
                     KKKTGLDALLEMILLQADVLELKANPNKPAVGAIIEARLDKGRGPVATVLVQDGTLRV
                     GDLFVTGAQSGKVRALLDDMGRRVDKAEPSTPVELIGLEGMPSAGDLFQVVKDDRTAK
                     DIVETRLGKARAAGVGGAAAPRKMTLEDLYGKMAEGGAKDLLIVLKADAQGSLEALRH
                     ALEKLSTDQVRLRVLHEGIGGITESDVLLAAASDAVIIGFSVRPDNKAQAVAEREKVD
                     IRTYRIIYEAVDNVRAAMEGLLAPTLKEQVLGRAEVRQVFMVPRAGAIAGSYVSQGVI
                     SRASAGVRVIRDGVVVYQGKLGSLRRFKDDVREVQTGYECGIGVENFNDIKVGDILED
                     FAIEKVPAKL"
     gene            complement(3556..4743)
                     /gene="nusA"
                     /locus_tag="AC16BC_08275"
     CDS             complement(3556..4743)
                     /gene="nusA"
                     /locus_tag="AC16BC_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR3241657.1"
                     /translation="MSRELLTVIGQIEREKGIKGEKILQAVELAVQTAARKKYGAADN
                     IQVRVDPETGDIEVVSLKKIVELVTNPKAEIALEDALAIDPGAELGDEIGDLMPMEDF
                     GRIAAQTAKQVIFQRVREAELETVYKEYSVRQGEIISGMILGQERRNYIVDLGKTEAL
                     LPVSEQIPREGFRRGDRLRAYLLEVKPSSRGPQLILSRACPEFVKKLFELEVPEVAEG
                     IVQIRGVVREPGDRTKICVSSRDKAVDPVGACVGVKGVRVQAVVRELRGEKIDIITWT
                     EDPRTFIGEALSPAVVEKVGITESEKSAIAVVADHQLSLAIGKKGQNVRLAAKLTGWK
                     IDIISQSEYEKERVKDRDREISAAIAHEQARLAAATGESGAAATAPVPTEQTQQADTE
                     SAS"
BASE COUNT          721 a         1807 c         1418 g          925 t
ORIGIN      
        1 gtggcatcgg gcacgatcca attcaatccg ccgaacccgc ggttggtgat gtggtggtcg
       61 atgttgacca cgagtttgac ccgcgggggc gccgactcga acatcccggt gcgcgccagg
      121 tccccgcaat ccaccaccgc cagcacgtcg tagccctgcg gcaacgtatt gtgccgctgc
      181 accacgccct ggtagggcag aaaatccagg aaccccggca cgggatcctt gatcaccacg
      241 tccgcggtct tgcccgcgga gcggagcgcg agcgccaacg cgatcgcgga gcccaatgcg
      301 tcgccttcgg ggctgacatg ggtggacacg ctgaaggacc gatggccctt gagcgcctcc
      361 accacggcgc ccgcgtcaat cgcggtgctc gtcgccatgt gcgtcgtctc ccgtctcgcc
      421 tcgcgcctcg ctcaactcat cgagcacggc ttccaagtgc gccgcgcggc tcggcgtggt
      481 gtcctgtttg aacaccaatt ccggcgtgta tttcatgcgc atccgccggc ccagttccgt
      541 gcgcacgaac cccgcggcgc ggtccagcgc cgccaaggtt tccgcggcgg gcgcgccctc
      601 gtccaacaga ctcacgaaca cggtggcgtg ccgcaggtcc gggctgacct tgaccccggt
      661 cacggtcaca aacccgaccc gcgggtcttt gacccgccgc agcaacacgt ccgcgatctc
      721 gttccgcatg agatcgccga cgcgatccgt ccgccgaaac cccgcgcgca tacgctcccc
      781 gactccattc acacgaactc caactcggac cgcacgatct ccacgccgtc ttcccgctcg
      841 accatgcgca ggagttggtc caagaccccg ttgacgtacg cgcgatccgt ccccacgcac
      901 gccgccgcca acgccgcccg ctgccacacg tcctggtcgc ccacttccgc gatcgacacg
      961 ttgaagtcct tccgaatccg atccttcaaa cttttgacca cgtagcgctt ctctttcaac
     1021 gagtgccggt ccggcaggtg caactcgacg cgacagaccc cgatcaccat gccgaatcga
     1081 ccggaggcta cagcttcgcc ggcaccttct cgatcgcgaa gtcctcgagg atatcgccca
     1141 ccttgatgtc gttgaagttc tccaccccga tcccgcactc gtatccggtc tgcacctcgc
     1201 gcacgtcgtc cttgaaccga cggagcgagc ccagtttgcc ctggtacacg acgaccccgt
     1261 cgcggatcac gcgcacgccg gcgctcgccc ggctgatcac gccctgcgac acatacgatc
     1321 ccgcgatcgc gcccgcgcgc ggcaccatga acacctgacg cacttcggcg cggcccagca
     1381 cctgttcctt gagcgtgggc gcgagcagcc cttccatcgc ggcgcgcacg ttgtccaccg
     1441 cttcatagat gatccggtac gtgcggatgt ccaccttctc gcgttcggcc acggcctgcg
     1501 ccttgttgtc gggacgcacg ctgaacccga taatcaccgc gtccgacgcg gccgccagca
     1561 acacgtcgct ctcggtgatg ccgccgatgc cctcgtgcaa cacgcgcaac cgcacctgat
     1621 cggtggacag cttctccagc gcgtgacgca aggcttccag cgacccctgc gcgtcggctt
     1681 tcagcacaat gagcaggtcc ttggccccgc cctcggccat cttgccgtac aaatcctcca
     1741 acgtcatctt gcggggcgcc gcggcgccgc ccactcccgc ggcgcgggcc ttgcccaatc
     1801 gggtttcgac gatatccttg gcggtacggt cgtccttgac gacctggaac aggtctccgg
     1861 ccgacggcat gccttccagc ccgatgagtt ccaccggcgt ggacggttcg gccttgtcta
     1921 cgcggcggcc catgtcatcg agcaacgcgc ggaccttgcc cgattgcgcg ccggtcacga
     1981 agaggtcccc gacccgcagc gtcccgtcct gcaccagcac cgtggccacc ggcccacggc
     2041 ccttgtccaa ccgcgcttcg atgatcgcgc ccaccgcggg tttgttcggg ttggccttca
     2101 actccaacac gtccgcctgc agcaggatca tttccagcaa cgcgtcgagc ccggtctttt
     2161 tcttggccga gacctcgacg aagatggtgg tcccgcccca ggcttccgat atgagcccgt
     2221 gctcggacaa ttgattcttc acgcgatcgg gctgcgcttc tggcttatcc atcttattga
     2281 gcgccacgat gatcggcgtg tcgcccgcct tggcgtggtt gatggcttcc acggtctgcg
     2341 gcatcacgcc gtcgtcggcc gccaccacca gcaccaccag gtccgtcacc ttggcgccgc
     2401 gcgcgcgcag gctggtgaac gcttcgtggc ccggcgtgtc caggaacgtg accgatttgc
     2461 cgtgtaccga caccgtgtac gcgccgatgt gctgcgtgat cccgcccgct tcctgctcgg
     2521 tcaccttggt cgagcggatc gcgtcgagca gcgaggtctt gccgtggtcc acgtggccca
     2581 tgatggtgac caccggcggc cggggcacgc gcatcccggg ctcgtccggc acggcctcga
     2641 tcatttcttc ttcggtgcgt tcggccacgc cttcggcctt gaccccgaag tgctccgcca
     2701 ccagcatcgc ggcctccaga tccacggtct ggttgatgct ggccgccgtg cccatggcca
     2761 tcaattgccc gatcacctgg gcgaccttca cccccaagag atccgcgaga tccttgaccg
     2821 tgagacccgc gcggagtttg accgcctttt gccgcggctt ggtcgcctcc acggtcggcc
     2881 cgccctggtg cgcgcgccgc aactgggccc gcaagcgctc gtcgcgacgg tgcagcggct
     2941 tgaagtcctg ccagaccaac gcgtcctgct gaagcggcac ggccgcggct tccgcttcct
     3001 tgtcgcgctt ggcgcctttc ttcggtccct tcttgagtcg ttccttttcc ttctcgcgct
     3061 ctttgaccaa tgtctcggtc gagaccgggc ctcggggcgg cgcagccgga gccgccggct
     3121 gaaccgcggc cggccgaatc ggttccggca tggggggctg cggcaccacg accggcgcgt
     3181 gcggacgaaa cacctcgggc gcctggcgag gcgcgggagc ccccggctga gagggcgccg
     3241 cgatctccgg cggaggcggc gcgaccggcg cttcgaccac cggcgcgggc ggagcttcgg
     3301 cctggacgcg tttcttgatc aggacttgac cgcggcgtgg ggccgcttcc ggcgcggcct
     3361 ccttcttggc cccgtatttc ttcatcaggg cctcgaccat gtcgtcctcc agcacgctca
     3421 tgtgattttt gagcgcggtg ccctgcttgg caagatccgc gagcagttcc ttgctcgtct
     3481 tgcccagttg cttcgccagc tcaaagactc tcatggccga atccttctgc gccgtcgtcg
     3541 ccgtcacgtc acctctcaag aagccgattc agtgtcggcc tgttgtgtct gttcagtcgg
     3601 aaccggcgcg gtcgcggcag cgccggactc cccggtcgcc gcggcgagcc tcgcctgttc
     3661 atgagcgatc gccgcgctga tctcgcggtc gcgatccttg acgcgctcct tctcgtactc
     3721 gctctggctg atgatgtcga tcttccaccc ggtcaatttg gcggccaggc ggacgttctg
     3781 ccccttcttg ccgatggcga gcgacagctg gtggtcggcc accacggcga tggcggactt
     3841 ctcggattcc gtgatcccga ccttttccac caccgcggga ctgagcgcct cgccgatgaa
     3901 cgtgcgcggg tcctcggtcc aggtgatgat gtcgatcttt tcgccgcgca gctcgcgcac
     3961 gaccgcttgc acgcgcacgc ccttcacgcc cacgcacgcc cccaccgggt ccaccgcctt
     4021 gtcgcgcgag gacacgcaga tcttggtccg gtcaccgggc tcgcgcacca cgccgcggat
     4081 ctgcacgatc ccttccgcga cttccggcac ttccagttcg aacaatttct tcacgaactc
     4141 ggggcaggcg cgggacaaga tcagctgcgg tccgcgcgag gacggcttga cctccaacag
     4201 gtacgcgcgc aagcggtccc cgcgtcggaa accctcgcgc ggaatctgct cgctcaccgg
     4261 caacaaggcc tcggtcttgc ccaggtccac gatatagttg cggcgctcct ggcccaggat
     4321 catcccgctg atgatctcgc cctgccggac cgagtactcc ttgtacacgg tctccaattc
     4381 ggcctcgcgt acccgctgaa agatcacctg tttggcggtc tgggccgcga tgcggccgaa
     4441 atcctccatc ggcatgagat cgccgatttc gtcgcccagc tcggcgccgg gatcaatggc
     4501 cagcgcgtct tccagcgcga tttccgcctt gggattggtg accaactcca cgattttctt
     4561 gagcgagacc acttcgatat ccccggtctc gggatcgacc cgcacctgaa tgttgtcggc
     4621 cgcgccgtac ttcttgcgcg cggcggtttg caccgccagc tccaccgcct ggaggatctt
     4681 ttcgcccttg atcccctttt cccgttcgat ttggccgatc accgtcaata attcacgact
     4741 catagactat ccctttacat gcagtttgcc gcagcgccga accgcccgca ccggacgccc
     4801 tgcggtgtca ccactcaact tccaacctgg cgtgcgcgat cgcgtccaac ggcagggacc
     4861 acagccgcgc c
//
LOCUS       DBNGTT010000369        10961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1109730, whole genome shotgun sequence.
ACCESSION   DBNGTT010000369 DBNGTT010000000
VERSION     DBNGTT010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10961
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1109730"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="AC16BC_08280"
     CDS             complement(<1..806)
                     /locus_tag="AC16BC_08280"
                     /inference="COORDINATES: protein motif:HMM:NF027583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HIR3241658.1"
                     /translation="MPSKRPRKRTPSPKGETGSRAKKPASSRKAGASKAPETAGSARS
                     RRAGAAKSAKAPNTPRPVGPRRAALLARAAAAGIAGRHRMTIPQLQAALARLAEPPSI
                     SSPPVAPDLRPLVVGSPPPVAGLPWQYGVTELVAMPVDPLLVYVYWELTPEAVARARA
                     DLGALWEGSQQVLRAYEVGNTLGGQNNGDTVVARARHHFDSDVGGDVGSYYVHLWSPE
                     QTLVFELGWRSRGGRFVAAVRSNLVRTPRNAPCGGEERWMTVRNGRIVTTP"
     gene            complement(796..2268)
                     /gene="glgA"
                     /locus_tag="AC16BC_08285"
     CDS             complement(796..2268)
                     /gene="glgA"
                     /locus_tag="AC16BC_08285"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR3241659.1"
                     /translation="MADALRILFAVSEVAPFSKTGGLGDVASALPRALSALGHDVRVV
                     TPRYRAFAGAVERLAVMRVSTPQGMVSATVGRAVLPDSAVPVYTVEDDRGWFDRDGLY
                     QSHSGDFPDNLERFAFFSRAALQVPSVVGWTPHVIHCHDWQTALIPLYVAAGSERPAV
                     RTVLTIHNMGFHGSFPGDRFPAIGIPAAWFSINGVEFFNCVNLLKGGLLAADLITTVS
                     PTYAEEIQTEPLSHGLHGVLATRRRDLVGILNGIDRKAWNPATDSLLAARFSASKPAG
                     KAACREDLLRRVNLTAGPAPVLALVTRLAEQKGVDLVIDLLPDLLLLDLRLVVLGTGD
                     GPYETALRHWAAKRPDRVAAVLAFDESMAHRIMAGADVFLMPSRYEPCGLSQMYAMRY
                     GAVPVAHQTGGLADTIVDYTPRTVHDGRATGFLFRPCAADNLLRAIQFALAVRRDRAA
                     WKRLVVNAMRADFGWEQSATQYVDAYRRALMKQPRRPLAE"
     gene            complement(2261..3247)
                     /gene="lipA"
                     /locus_tag="AC16BC_08290"
     CDS             complement(2261..3247)
                     /gene="lipA"
                     /locus_tag="AC16BC_08290"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR3241660.1"
                     /translation="MSDLIQITRGPTRRGKRPAHVTESAARQPLPPWIRVRLHQGPDL
                     ARLRKTFREATLHTVCEEARCPNLWECWNRGTATFMILGDVCTRSCGFCAVKSGRPTD
                     TDRDEPRRVAEAVASMNLRHAVVTSVNRDELADGGAAVFAETIRAIRRAKPDCTVEVL
                     IPDFQGSEAALITVLDARPDLLNHNTETVPRLYATVRPQARYDRSLEVLRRAKTAGAV
                     TKTGLMVGLGETADELLAVFADLRGAGCDILTVGQYLQPTREHLPVVRYYDPPEFDAL
                     RDHAVRLGFAHVEAGPLVRSSYHADDQATSAAASDGTTGRSLRPAFDEAPRG"
     gene            complement(3467..5380)
                     /gene="dxs"
                     /locus_tag="AC16BC_08295"
     CDS             complement(3467..5380)
                     /gene="dxs"
                     /locus_tag="AC16BC_08295"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR3241661.1"
                     /translation="MMRLLDQINAPEDLRRLSPEQLPQVAAELRDTIINTLAANGGHF
                     AGPLGAVDLNVALHYVFDTPRDRLVWDVGYQAYAHKLLTGRRERFHTLRQLDGISGFP
                     LRAESSYDTFGTGHAATSISAALGMAEGRDLLPEGGDVTPRKVVAIIGDGSMTAGMAF
                     EGLNQAGALKTDLIVVLNDNSMAISENVGALSSYLTRIITGKLYTKVKEETEAILRHI
                     PKIGPPMLKMAKKAEESMKGLFVPGLLFEEMGFRYIGPIDGHRLDHLVTTFENVKHLS
                     GPLLVHVITHKGKGYEPAERNPIAFHGVTPFDRETGRSKKAPGRPSYTTIFANTLIEL
                     ARSRPEVVAITAAMPEGTGLNLFAKEFPDRYYDVGIAEQHAVTFAAGLATEGVRPVVA
                     IYSTFLQRGYDQVVHDVCLQNLPVLFCLDRGGLVGEDGATHHGVFDFSYLRHIPNMVV
                     MAPKDENELRHMLLTGLLHPGPCAIRYPRGESEGVTPDPTIVPLPIGRGEIVRDGDDV
                     ALVAIGAMVPVALKAADLLRDRGVAATVVNARFVKPLDRDLLLDVAERVRRIVTIEDQ
                     ALMGGFGSAVLELFEEADARGVEVRRIGLPDAFIEHGAQKLLRQQHGLDPEGVAQRVM
                     AFLKSPLEVSEPV"
     gene            complement(5374..6315)
                     /locus_tag="AC16BC_08300"
     CDS             complement(5374..6315)
                     /locus_tag="AC16BC_08300"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3241662.1"
                     /translation="MPATSANPPITTYLDDRRALVDRALRDALPGARTPSVLREAVTY
                     SVMAGGKRIRPILALAACDAVGGDSRRIVPLVVSLEFVHTYSLIHDDLPAMDDDDLRR
                     GRPTNHRVFGEDMAILAGDALQAMAFAALSDARDPDDIPAAVRLAAVAELAAASGAEG
                     LAGGQALDLRADAPPAAAPADEAARAAALATLDDIHARKTGALIRAAVRMGAILGGAT
                     EARLLALTRYGEAVGLLFQIADDVLDVEATAEEMGKATGKDRDRHKWAYPAVIGVAAS
                     REIAEACVRDAIEALSGFSEAADPLRGVARYILDRRS"
     gene            complement(6296..6559)
                     /locus_tag="AC16BC_08305"
     CDS             complement(6296..6559)
                     /locus_tag="AC16BC_08305"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR3241663.1"
                     /translation="MAVLKFEDALGRLETIVQTLERGELSLDESLKAFEEGVKLSKNC
                     LKLLDDAERRVDILVGERGRKKTQPFPTDDDTDPETTDAGDER"
     gene            complement(6610..8025)
                     /locus_tag="AC16BC_08310"
     CDS             complement(6610..8025)
                     /locus_tag="AC16BC_08310"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3241664.1"
                     /translation="MDTSSVPEAESAPGESGGPTASRLTTSTTFHSLPIAPEVLQGLD
                     QLGYTHCTPIQERTLPITLAGKDVAGQAQTGTGKTAAFLISVFTRLLRTPPPPKSARS
                     SPRALVVAPTRELAVQIEQEARAVGAFTGLSMMAVYGGVDYTKQRDDLAAGVDLLVGT
                     PGRLIDYFKQKVYDLRRTEIVVIDEADRMFDMGFIADIRYLLRRMPPYDKRQSMLFSA
                     TLSMRVMELCYEHMNNPEKVSVTPQRITADKIQEALYHVERSRKFSLLAGLLKREPWE
                     RVLIFVNTKHVGERLAEQFNHHGYTARAITGDIPQPKRLKYLERFKKGEIKILVATDV
                     ASRGLHIEGVSIVVNYDIPQDPEDYVHRIGRTARAGAAGKAISLACEEYAMGLEAIEA
                     YIGRKIPVEWAEDDWFAKVTSAPRRPRPSEGGAGPRRASHGPASRPGARSTAGSASAS
                     SGRGPRRRRGPRSGTPPPAAS"
     gene            complement(8072..8863)
                     /locus_tag="AC16BC_08315"
     CDS             complement(8072..8863)
                     /locus_tag="AC16BC_08315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR3241665.1"
                     /translation="MDCAPHRLNGDALAASPPAPDATWKTRVRIVLVRPEHAGNIGAA
                     ARAIKNFGLSRLDLVAPECGHLTSDSLKMAYGARDVIEDARIHATLREALTDVRWAAA
                     FGMARPDRPDAVWLADAAPGLADRAQAHDTALVFGPERSGLSNDELALCHQLVAVPTD
                     PAQPSLNLAQAVLLGCYELTRGSLAEAAAPRDLASGAEIDGLTDHLDATLHAIGFLQP
                     PQGRRLLHELRAFIHRAQPSPREVRVFRGILRQIGWAIRNARGTD"
     gene            9047..9817
                     /gene="rpsB"
                     /locus_tag="AC16BC_08320"
     CDS             9047..9817
                     /gene="rpsB"
                     /locus_tag="AC16BC_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR3241666.1"
                     /translation="MSDIGLKDLLEAGVHFGHQTKRWNPKMKKYIFGERNGVYIIDLQ
                     KTVKKFGEACAFVENAVADGATVLFVGTKRQAQEIVESEAKRCGMYYVNQRWLGGMLT
                     NFQTLRKSADKLKKLDAAKTDGSLERRTKKEAAGMEKERAQLEKILGGIKTMTSLPGI
                     IFLIDCKKENIAVHEANRLGIPVVAIVDTNCDPDQVDYVIPGNDDAIRGLRLITGRIA
                     DAVIEGRRLHAARIEEQAKANAVSAGHAMAELAAAEGR"
     gene            9820..10428
                     /gene="tsf"
                     /locus_tag="AC16BC_08325"
     CDS             9820..10428
                     /gene="tsf"
                     /locus_tag="AC16BC_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR3241667.1"
                     /translation="MAAIDPALVKQLRERTGAGMMDCKKALEQHGGDLEKAADWLREQ
                     GAAVAAKKAGREAADGLIGSYIHAGGKIGVLVEVNCETDFVARNPEFQALVKDLAMHV
                     AGTPTTPQYISRKDIPESALTEARARFTEEAKQSGKPEKVVAQIVQGRIDKFSAEISL
                     LDQPFIKDPTVTIDALITEKVAKIGERISVRRFIRYKLGEDV"
     gene            10493..>10961
                     /locus_tag="AC16BC_08330"
     CDS             10493..>10961
                     /locus_tag="AC16BC_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR3241668.1"
                     /translation="MADLKYRRVLLKLSGEVLAGERGYGIDPVVVGEIADQIQEVNAL
                     GVELAIVIGGGNIFRGLAAAATGIERSSADYMGMLATVMNALALQSVLERKGVLTRVQ
                     SAIEMRQLAEPYIRRRAIRHLEKKRVVILACGTGNPYFSTDTAAALRAMEMGAE"
BASE COUNT         1826 a         3580 c         3819 g         1736 t
ORIGIN      
        1 ggcgtggtca cgatccggcc gtttcgcacg gtcatccacc gttcctcccc gccgcagggc
       61 gcgttgcgcg gagtacggac gaggttggaa cgcacggcgg ccacgaagcg ccccccccga
      121 ctgcgccagc cgagctcgaa caccaaggtt tgctccggac tccacaggtg gacgtaataa
      181 ctgcccacgt cgccgcccac gtccgaatcg aagtgatgac gcgctcgcgc cacgacggta
      241 tcgccgttat tctggccccc gagcgtgttg cccacctcat aggcccgcaa tacctgctga
      301 gatccctccc acagggcgcc gaggtcggcg cgcgcgcggg cgacggcctc gggcgtgagc
      361 tcccaataca cgtagacgag gagcggatcc accggcatgg ccaccaattc ggtgacgccg
      421 tactgccagg gaagcccggc gaccggaggc ggcgagccca cgaccagcgg ccggaggtcc
      481 ggcgcgacgg gcggcgatga gatggacggc ggttccgcga ggcgcgcgag cgcagcctgc
      541 aattggggga tggtcatgcg gtgacgtccc gcgattcccg cggcggcggc ccgggccaac
      601 aacgcggcgc gccgagggcc gacgggccgc ggcgtgttcg gcgccttcgc ggacttggcc
      661 gcgccggcgc gccggctccg agcggaccct gcggtctccg gcgccttcga tgcgccggcc
      721 ttccggctcg acgccggttt cttcgcgcgg gagccggtct cccccttcgg ggacggcgtg
      781 cgtttccggg gtcgtttact cggcaagggg cctccttggt tgtttcatca gcgcccggcg
      841 gtaggcgtcc acgtactgcg tcgcggattg ttcccacccg aagtcggcgc gcatggcgtt
      901 gaccacgagc cgcttccacg cggcccgatc ccggcgtacg gcgagcgcga actggatcgc
      961 ccggaggaga ttgtcggccg cgcacgggcg aaagagaaat ccggtcgcgc gtccgtcgtg
     1021 aaccgtgcgc ggcgtgtagt cgacgatcgt gtccgcgagt ccgccggttt ggtgcgcgac
     1081 cggcacggcg ccgtaacgca tcgcgtacat ctggctgagc ccgcagggct cgtaccgcga
     1141 gggcatgagg aatacgtccg cccccgccat gatccgatgg gccatggatt cgtcgaaggc
     1201 gagcacggcc gcgacgcgat cggggcgttt ggcggcccag tgccggagcg cggtctcgta
     1261 cgggccgtca ccggtgccga gcacgaccag gcgaaggtcc aggagcagca agtccggcaa
     1321 caggtcgatc accaggtcca cgcccttctg ctcggcgagc cgcgtgacca aggcgagtac
     1381 cggggcggga cccgcggtga ggttcacgcg gcgcaacaga tcctcgcgac aggcggcctt
     1441 gccggccggc ttggacgcgg agaagcgggc cgccagcagc gaatcggtcg cggggttcca
     1501 ggccttgcga tcgatgccgt tcaaaatccc gacgaggtcg cgtcgccgcg tcgccagcac
     1561 gccgtgcaac ccgtggctca acggctcggt ctggatttct tcggcgtagg tgggactgac
     1621 ggtggtgatg agatccgcgg cgagcagccc gcccttcagg aggttgacgc aattaaagaa
     1681 ctccaccccg ttgatggaga accaggccgc gggaatcccg atcgccggaa agcgatcccc
     1741 gggaaacgag ccgtggaacc ccatgttgtg aatggtcagc accgtgcgaa ccgcggggcg
     1801 ctcgctgccc gccgccacat acagcgggat caatgcggtc tgccagtcgt ggcaatgaat
     1861 gacgtgcgga gtccaaccca ccacggacgg gacttggagc gcggcgcgcg aaaaaaacgc
     1921 gaatcgttcc aggttgtccg gaaagtctcc ggagtgggac tgatacaagc cgtcgcggtc
     1981 gaaccacccg cgatcgtcct ccacggtata caccgggacc gcgctgtccg gcagcacggc
     2041 gcgtccgacc gtggcggaca ccatgccttg cggcgtggac acgcgcatga ccgcgaggcg
     2101 ttccaccgcg cctgcgaacg cgcgataccg aggcgtcacc acgcgcacat catgtcccag
     2161 cgcggacagg gcgcgcggca gcgccgacgc cacgtcgccc aagccgccgg tcttggaaaa
     2221 cggggcgact tcggagaccg caaagaggat gcggagcgcg tcagccacgc ggggcctcgt
     2281 cgaaagcggg acggagcgaa cggccggtcg tcccgtcaga cgctgcggcg gacgtcgcct
     2341 ggtcgtccgc gtgatacgag ctgcgcacca gggggccggc ctcaacgtgc gcaaacccca
     2401 gtcgaaccgc gtgatcgcgc agggcgtcga attccggagg gtcgtagtag cgcacgaccg
     2461 gcaggtgctc gcgcgtgggt tgcaggtact ggccgacggt gaggatgtca cagcccgcgc
     2521 cgcgcaggtc ggcaaacacc gccaacaatt catcggcggt ttcgcccagc cccaccatca
     2581 gcccggtctt cgtcaccgcg cccgcggtct tggcgcgacg cagcacttcc agggaccggt
     2641 cgtaacgcgc ttgcggccgg accgtcgcgt agagacgggg gacggtttcg gtgttgtgat
     2701 tgaggaggtc cggccgggcg tccagcaccg ttatgagcgc cgcctcggac ccctgaaaat
     2761 cagggatcaa gacttcgacc gtgcagtccg gtttggcccg acggatcgcg cggatcgttt
     2821 cggcaaacac cgcggccccg ccgtcggcca actcgtcgcg gttgaccgag gtcaccacgg
     2881 cgtggcgcag gttcatggac gcgaccgcct cggccacgcg ccgcggttcg tcccgatccg
     2941 tgtccgttgg tcggccggac ttgaccgcgc aaaacccgca cgagcgggtg cacacgtcgc
     3001 ccaggatcat gaacgtcgcg gtgccgcggt tccagcactc ccacaggttg ggacagcgcg
     3061 cctcttcgca caccgtgtgc agggtcgcct cccgaaaggt ttttcgcaga cgcgcgaggt
     3121 cggggccttg gtgcaagcgc acgcgaatcc acggtggcag cggctgccgt gccgcggact
     3181 cggtgacgtg cgcgggccgt ttaccgcgcc gcgtaggtcc tctggtgatc tggatcagat
     3241 cgctcatggg gtagtagttg ccgtggtgtt cccggcgtca cgccgtatgc gacgccggag
     3301 cgtcgctcag gaatcggcca gatgccccct tcggggactt ccggcattgc tctcctcgaa
     3361 ggtgggtcgc aaggcgccgc gagcaccggc ccggagcgta ctgggtcgta cgtgaggacc
     3421 ggcagcgcag cggcaacgcc gcagatggcc gttcatgagc acgctcttag accggctccg
     3481 aaacttcgag cggggacttg aggaacgcca tcacgcgctg cgccacgcct tcagggtcca
     3541 ggccgtgctg ttgtctcagg agtttctggg ctccgtgctc gatgaacgcg tccggcaagc
     3601 cgatccgtcg cacctccact ccgcgcgcgt ccgcttcctc gaacagttcc agcaccgcgc
     3661 tgccgaaccc tcccatcaac gcctgatcct cgatcgtgac gatgcgccgg acgcgttccg
     3721 ccacgtcgag gagcaagtcg cggtccaggg gtttgacgaa ccgggcgttg accacggtcg
     3781 cggccacgcc gcgatcgcgc aacaggtccg cggccttgag cgccaccggc accatggcgc
     3841 cgatcgccac cagcgccacg tcgtcgccgt cacggacgat ctccccgcgg ccgatgggca
     3901 acggcacgat cgtgggatcc ggggtcacgc cctcggactc gccgcgcgga tagcggatcg
     3961 cgcagggccc cgggtgcagc aggccggtga gcagcatgtg gcgcaactcg ttttcgtcct
     4021 tgggcgccat gacgaccatg ttcggaatgt gccgcaaata cgagaagtcg aacacgccgt
     4081 ggtgggtggc tccgtcttcg cccaccaggc cgccgcggtc caggcaaaac agcaccggga
     4141 ggttttgcag gcacacgtcg tggaccacct gatcgtaccc gcgctgcagg aaggtggaat
     4201 agatcgcgac cacgggccgg acgccctcgg tcgccaggcc cgcggcgaac gtcaccgcgt
     4261 gctgctcggc gatccccacg tcgtaatacc gatccgggaa ttccttggcg aacagattca
     4321 aacccgtgcc ttccggcatg gccgcggtga tggcgacgac ttcgggccgc gagcgcgcga
     4381 gctcgatgag cgtgttggcg aagatcgtgg tgtacgacgg tcgccccgga gctttcttgg
     4441 agcggccggt ctcccgatcg aacggcgtca cgccgtgaaa cgcgatggga ttgcgttcgg
     4501 ccggctcgta tcccttgccc ttgtgcgtga tgacgtggac caacagcggt cccgagaggt
     4561 gtttgacgtt ctcgaacgtc gtcaccagat ggtccaggcg gtgaccgtcg atgggaccga
     4621 tataccgaaa gcccatttcc tcgaacagga gcccggggac gaacaggcct ttcatcgact
     4681 cctcggcctt cttggccatc ttgagcatgg gcgggccgat cttgggaatg tggcgcaaga
     4741 tcgcctcggt ttcttccttg accttggtgt acagcttccc cgtgatgatc cgggtcaaat
     4801 aactcgacag cgcgccgacg ttttccgaga tggccatgga gttgtcgttc aagaccacga
     4861 tcaagtcggt cttgagcgcg ccggcctggt tgagcccctc gaacgccatg ccggcggtca
     4921 tggagccgtc gccgatgatc gccaccacct tgcgcggcgt cacgtcgccg ccttcaggca
     4981 gcaggtcccg gccctcggcc atgccgagcg cggccgaaat cgacgtggcc gcgtggcccg
     5041 tgccgaaggt gtcgtacgaa ctctcggcgc ggaggggaaa gccgctgatg ccgtcgagtt
     5101 ggcgcaaggt gtgaaaccgc tcgcgccggc ccgtcagcag cttgtgcgcg taggcctggt
     5161 agccgacgtc ccagaccagc cggtcgcgcg gggtgtcgaa cacgtaatgg agcgctacgt
     5221 tcaggtccac cgcgcccagg ggccccgcga aatggccgcc gttcgcggcg agcgtgttga
     5281 tgatggtatc gcgcaactcg gccgccacct gcggaagctg ctccggcgac aggcggcgga
     5341 ggtcctccgg cgcgttgatc tggtcgagca gcctcatgat cgtcgatcca ggatgtaccg
     5401 cgcgacgccc cgcaacgggt ccgcggcctc ggaaaaaccg gacaaggctt cgatcgcgtc
     5461 gcggacgcac gcttccgcga tttcgcggga cgcggcgacg ccgatcaccg cgggatacgc
     5521 ccacttatgt cggtcgcgat ccttgcccgt ggccttgccc atttcttcgg ccgtggcttc
     5581 gacgtccaac acgtcgtccg cgatctgaaa gagcaggccc acggcttcgc cgtagcgcgt
     5641 cagcgcgagg aggcgcgcct cggtggcgcc gcccaggatc gcgcccatgc gcaccgcggc
     5701 gcggatcagc gcgccggtct tgcgcgcgtg aatgtcgtcg agggtcgcga gtgccgcggc
     5761 acgggccgct tcgtcggcgg gtgccgcggc gggcggggcg tcggcgcgca ggtccaacgc
     5821 ttgtccgccc gccagtcctt ccgcgccgga agccgcggcc agttccgcca cggcggccag
     5881 ccgcaccgcg gccgggatgt cgtcggggtc ccgggcgtcc gagagcgcgg cgaacgccat
     5941 cgcctgcaac gcgtcgccgg ccaggatcgc catgtcttcc ccgaagacgc gatggttcgt
     6001 ggggcggccc cgacgcagat catcgtcgtc catggccggg agatcgtcgt gaatcaacga
     6061 gtaggtgtgc acgaattcca gggaaaccac caatggcacg atgcggcggc tgtcgccccc
     6121 gaccgcgtcg cacgcggcga gcgcgaggat cggccgaatg cgcttgccgc cggccatgac
     6181 gctgtaggtc acggcctccc gcaagacgga cggagtcctg gcgccgggca gcgcgtcgcg
     6241 caacgcccgg tccaccaatg cgcgccggtc gtccagatac gttgtgatcg gcgggttagc
     6301 gctcgtcgcc ggcatcggtg gtctctgggt cggtgtcgtc gtccgtggga aacggctggg
     6361 tcttcttgcg tccccgctcc cccaccaaga tgtccacgcg gcgctccgcg tcgtcgagca
     6421 gcttgaggca gttcttggaa agcttgacgc cctcttcgaa cgccttcagg gattcgtcga
     6481 gtgaaagctc gccgcgttcg agggtctgga cgatggtttc gaggcgtccc agtgcgtctt
     6541 caaacttcaa cacggccatc ggagcactcc gcgacaggtg tgcatcttaa acaacgcggg
     6601 agggggcggt caagacgccg cggggggcgg cgtgccagac ctgggacctc gacgccgtcg
     6661 cggcccgcgg ccggacgaag ccgacgccga gccggcggtc gatcgcgcgc cggggcgcga
     6721 agcggggccg tggcttgcgc gtcgcggacc agccccgccc tcagagggac gcgggcgccg
     6781 tggggccgag gtgaccttgg caaaccagtc gtcttccgcc cactccaccg ggattttgcg
     6841 tccgatgtac gcttcgatcg cttcgagccc catcgcgtat tcctcacagg ccaaggagat
     6901 ggccttgccc gcggcgccgg cgcgggcggt gcggccgatg cgatgcacgt aatcttcggg
     6961 gtcctggggg atgtcgtagt tcaccacgat cgacacgccc tcgatatgta atccgcgcga
     7021 ggccacgtcg gtggcgacca ggatcttgat ctcccccttc ttgaaccgct cgaggtactt
     7081 gaggcgcttg ggctgcggga tgtcgccggt gatcgcccgc gcggtgtacc cgtggtgatt
     7141 gaactgctcc gcgagacgtt cgcccacgtg tttggtgttc acgaagatca acacgcgctc
     7201 ccacggttcg cgtttgagca agccggccaa caaggagaac ttgcgggagc gttcgacgtg
     7261 atacagcgct tcctggattt tatccgcggt gatccgctgg ggcgtgaccg acaccttctc
     7321 ggggttgttc atgtgctcgt aacacagttc catcacgcgc atggacaacg tggccgagaa
     7381 caacatggac tggcgtttgt cgtagggcgg catccgccgc agcagatacc ggatgtccgc
     7441 gatgaacccc atgtcgaaca tgcggtcggc ctcgtcgatc accacaatct cggtgcgccg
     7501 caggtcgtaa accttttgtt tgaagtagtc aatgagccgg cccggcgtgc cgaccaggag
     7561 atccaccccc gcggcaaggt cgtcgcgctg tttggtgtag tccacgccgc cgtagaccgc
     7621 catcatggac aggccggtaa acgcgccgac cgcccgggct tcctgctcga tttgcacggc
     7681 cagctcgcgc gtgggcgcca ccaccaatgc ccgcggactg gagcgcgcgc tcttgggcgg
     7741 tggcggcgtg cgcagcagcc gggtaaacac gctgatcaaa aacgccgcgg tcttgcccgt
     7801 gccggtctgc gcctgtcccg ccacgtcttt gcccgcgagc gtgatcggca gcgtgcgttc
     7861 ttgaatcggc gtgcagtggg tatagcccaa ctggtcgagc ccctgcagga cctcgggcgc
     7921 gatcgggagc gagtgaaagg tggtcgaggt cgtgaggcgg gacgcggtcg ggccgccgga
     7981 ctcgcccggg gccgactcgg cctcgggaac agatgaagtg tcaatcacgc cgcgcatcat
     8041 accaaagcgg cgcgcggacg cgcaaggcga gtcagtccgt cccgcgcgcg ttgcggatgg
     8101 cccagccgat ttggcggagg atgccgcgga acacgcggac ttcgcgcggg gagggttgcg
     8161 cgcggtggat gaaggcgcgt agttcgtgta gcaggcggcg gccttggggc ggctgcagga
     8221 agccgatcgc gtgcagcgtg gcgtccaggt ggtcggtgag gccgtcgatc tccgcgccgg
     8281 aggccagatc gcggggggcg gcggcctcgg caagacttcc gcgcgtaagc tcgtaacagc
     8341 ccagcagcac ggcctgcgcg aggttgagcg agggctgcgc cgggtcggtg ggcacggcca
     8401 cgagttggtg gcacaacgcg agttcgtcgt tggacaggcc ggagcgctcc gggccgaaca
     8461 ccaatgcggt gtcgtgcgcc tgcgcccgat cggcgagccc gggcgcggcg tcggcgagcc
     8521 agaccgcgtc gggtcggtcg ggtcgcgcca tgccgaaggc cgcggcccat cgcacatcgg
     8581 tgagcgcctc gcgcagggtc gcgtggatgc gggcgtcttc gatcacgtcg cgcgcgccat
     8641 aggccatctt gagcgagtcg gacgtgaggt gcccgcactc gggagcgacc aggtccaggc
     8701 gggacagtcc gaagttcttg atggcgcgcg ccgcggcccc gatgttgccc gcgtgttccg
     8761 gccgcacgag cacgatccgg acgcgcgtct tccacgtggc gtcgggcgcc ggagggctcg
     8821 ccgcgagggc gtctccattc agtctgtgcg gggcgcaatc catcgcagtc cgacgcgagt
     8881 atacgccttg caaaaggccc ccgtctatgg taagacaggc cgttcaaacg gggcggcggt
     8941 cgccccaaat ctcacacgcg cgcggagggc gccggcggtc ccgcatcgcc gggtccggcg
     9001 tccgttgaag cgcgcggcgg atcaacttca cggggaggag tacggtatgt ctgacatcgg
     9061 gctgaaagat ctcctggagg ccggcgtgca tttcggccac cagaccaagc ggtggaaccc
     9121 caagatgaag aaatacatct tcggggaacg caacggcgtc tacatcatcg acctgcagaa
     9181 aacggtcaag aagttcggtg aagcctgcgc gttcgtggaa aacgcggtgg ccgacggcgc
     9241 gaccgtgctg ttcgtgggaa ccaagcggca ggcgcaggag atcgtggagt cggaagccaa
     9301 acgctgcggg atgtattacg tcaaccagcg gtggctgggc ggcatgttga ccaacttcca
     9361 gaccctgcgc aagagcgcgg acaaactcaa gaagttggac gcggccaaga ccgacggcag
     9421 cctcgagcgg cggacgaaga aggaagccgc ggggatggag aaggagcggg cccaactgga
     9481 gaagatcttg ggcggcatca agaccatgac ctccttgccg ggcatcattt ttctcatcga
     9541 ttgcaagaag gaaaatattg cggtgcacga ggcgaaccgt ctcggcattc ccgtggtggc
     9601 gatcgtggac actaattgcg atcccgacca ggtggactac gtcatccccg gaaacgacga
     9661 cgcgattcgc ggactgcggc tgatcaccgg acgcatcgcg gacgcggtga tcgaggggcg
     9721 tcggttgcac gccgcccgga tcgaggagca ggccaaggcg aacgcggtgt ccgcggggca
     9781 cgcgatggcg gagttggccg ccgcggaggg ccggtagcaa tggcggcgat cgatcccgcg
     9841 ctcgtcaagc agttgcgcga gcgcaccggc gccggcatga tggactgcaa gaaggcgctg
     9901 gaacaacacg gcggcgatct ggaaaaggcc gcggactggc tgcgcgagca gggcgcggcg
     9961 gtggcggcca agaaagccgg ccgcgaagcc gcggacgggt tgatcggttc gtacatccac
    10021 gccggcggca agatcggcgt gttggtggag gtcaactgcg aaaccgattt cgtggcccgc
    10081 aaccccgagt tccaggccct ggtcaaggac ctggccatgc atgtggccgg aaccccgacc
    10141 acgccgcaat acatcagccg taaggacatc cctgaaagcg ccttgaccga ggcgcgcgcg
    10201 cggtttaccg aggaagccaa gcagtcgggc aaacccgaga aggtcgtcgc gcagatcgtg
    10261 caaggccgca tcgacaagtt cagcgccgag atctcgctgc tggatcagcc gttcatcaag
    10321 gatccgaccg tgacgatcga cgctttgatc acggaaaagg tcgccaagat cggcgagcgt
    10381 atttccgtgc gccggttcat tcggtacaag ctgggagaag acgtataagc cggcggtgcg
    10441 atcgcctgcg atccgtcgtc ggcaccttga cggctgtgtc cctggatacc ccgtggccga
    10501 cctgaaatat cgtcgagtgt tgttgaagct gagcggcgag gtgctggccg gcgaacgcgg
    10561 ttacgggatc gaccccgtgg tggtggggga gatcgctgac cagatccaag aggtcaatgc
    10621 cctcggcgtg gaactggcca ttgtcatcgg cggcggcaac atcttccgcg ggctcgcggc
    10681 cgcggccacc gggatcgagc ggtcctcggc cgactacatg ggcatgctcg ccaccgtcat
    10741 gaacgcgctg gcgctgcaaa gcgtgttgga gcgcaagggc gtgctcacgc gggttcagtc
    10801 cgccatcgag atgcggcagt tggccgagcc gtacatccgg cgccgcgcga tccggcatct
    10861 ggaaaagaaa cgcgtcgtca tcctcgcgtg cggcaccggc aatccctatt tttcgaccga
    10921 caccgcggcc gccctgcgcg ccatggagat gggcgcggag g
//
LOCUS       DBNGTT010000370         2943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_216101, whole genome shotgun sequence.
ACCESSION   DBNGTT010000370 DBNGTT010000000
VERSION     DBNGTT010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_216101"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /gene="rplS"
                     /locus_tag="AC16BC_08335"
     CDS             complement(<1..342)
                     /gene="rplS"
                     /locus_tag="AC16BC_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR3241669.1"
                     /translation="MDQMQRIEKRWLKSDAPKFQVGDTVRVHVKVIEGEKERVQVFEG
                     VVIAKKGTSNRETFSVRKVSYGVGVERVFPVHSPFVEKVEVVRSGRVRRAKLYYLRSR
                     QGRAARIAERGA"
     gene            complement(401..1162)
                     /gene="trmD"
                     /locus_tag="AC16BC_08340"
     CDS             complement(401..1162)
                     /gene="trmD"
                     /locus_tag="AC16BC_08340"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR3241670.1"
                     /translation="MRCEIITLFPDMVRAVLGASILRRACEAGLVDVRVIDLRDYTVD
                     RHRTADDVPYGGGAGMVLKVEPVARAVDALRTDGEPRRWLLTSPRGRSFSHELATEFS
                     REGRCLTLLCGHYEGVDERIHGTAEWEDVSIGDYVLTGGELAALVMLDATARLVPGVL
                     GDPRSAHEESFVHSLLEYPQYTRPAEFRGMRVPEVLLSGHHQAISQWRRDQSLQDTRR
                     RRPDLFERVAATEEGRSRVVSDAGGSSADLADGMN"
     gene            complement(1165..1719)
                     /gene="rimM"
                     /locus_tag="AC16BC_08345"
     CDS             complement(1165..1719)
                     /gene="rimM"
                     /locus_tag="AC16BC_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3241671.1"
                     /translation="MTSVAFKNGPAAVAVGPASPVVVARVVKPIGLKGAVRVEAMSDA
                     PDRFTAGARFWVLSTPPVRVTLAEAAGAPDGRLVLRFEGRESIDAVSAWRGCFLAIEE
                     SERAPLPDGAFYHDELKGMSVETEAGILVGVVRDVLSAGPYDLLAIEDGAQERLLPMV
                     KAFVIRLDRALRRVTVRPPAGWLD"
     gene            complement(1716..1976)
                     /gene="rpsP"
                     /locus_tag="AC16BC_08350"
     CDS             complement(1716..1976)
                     /gene="rpsP"
                     /locus_tag="AC16BC_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR3241672.1"
                     /translation="MVKIRLARQGRHKRPLYRIVVADSRSPRDGRFIEVLGTYDPLAA
                     SAAVRVNAERANGWLARGAQVTDTVRQLFKRSGVGSASPRAV"
     gene            complement(2016..>2943)
                     /locus_tag="AC16BC_08355"
     CDS             complement(2016..>2943)
                     /locus_tag="AC16BC_08355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3241673.1"
                     /translation="RRSRPPAGVVRWLDCFPHNVAVVAPRRTQERPVGFQIDQERFTE
                     LFRHASDGVIVIDSSLRIVAINPAAEQLTACPAKAAVGIKICSEVAVCQNAEGFELCE
                     TACPGQAAFSRGERRLDLEVNLPGRGGQTAPAACVPLKSVGGESLAMILVRDVSDRAA
                     LEEHVLSVERVDRVSGFYARSYFDELYRREVRLAERQQRALAAVVVSLSGDALEGPDA
                     DRRIRGVAEAIRASLRRVDIAGRYDHGTFVVVLLDADSSGAASLIARIRAKVPSQSVA
                     EGLSITYGVGTAAINGYAGLLKAAKQSLKGSL"
BASE COUNT          571 a         1092 c          803 g          477 t
ORIGIN      
        1 ggcgccgcgc tcggcgattc gagccgcccg cccttgtctg gaccggagat aatacaattt
       61 cgcccggcga acgcgccccg aacgaaccac ctcaaccttc tctacaaacg gcgaatgcac
      121 cgggaacacg cgctcgaccc cgaccccgta ggacactttg cgaaccgaga acgtctcgcg
      181 gttactggtg cccttcttgg cgatgacgac cccttcgaac acctggaccc gttctttctc
      241 gccttcgatc accttgacgt ggacgcgcac cgtatccccc acttgaaatt tgggagcgtc
      301 ggatttgagc catcgttttt caattcgttg catctgatcc atcggaatcc tccttaccga
      361 aacacgcgag cagacgtctc gcgcacactg tgtagcgtct ctaattcatt ccgtcggcga
      421 gatccgccga cgagcctccg gcatccgaaa ccacccgcga tcgtccctcc tccgtggccg
      481 ccacccgctc gaatagatcg gggcggcgac gacgggtatc ctgaagcgac tggtcccggc
      541 gccactgcga aatcgcttgg tgatggccgg acagcaacac ctccggcact cgcatgcctc
      601 gaaactcggc gggacgcgtg tactgcggat actccagcaa ggagtggacg aacgattcct
      661 catgggccga tcgggggtca cccaacaccc caggcaccag ccgcgccgtg gcgtcgagca
      721 tcaccagcgc cgccaattca cccccggtca ggacgtaatc gccgatcgag acgtcctccc
      781 actcggcggt gccatggatc cgctcatcaa ccccctcgta gtgaccgcac aacagcgtca
      841 gacaccggcc ttcacgactg aattcggtcg ccagttcatg ggaaaaggac cggccccgag
      901 gagacgtgag gagccatcga cgcggctcac cgtcggtgcg caaggcgtcc acggcgcggg
      961 ccaccggctc gaccttcaac accattcccg cgccgcctcc atacgggacg tcatccgcgg
     1021 tgcggtgccg atccacggtg tagtcgcgta gatcaatgac gcgcacgtcc accagccccg
     1081 cctcgcacgc gcgacgcagg atgctggcgc ccaacaccgc tcggaccatg tcgggaaaga
     1141 gcgtgatgat ctcacaccgc aacatcagtc taaccatccc gccggtggtc gcacggtgac
     1201 ccgtcggagc gcccgatcca agcggattac aaaggctttg accatcggta aaagccgctc
     1261 ctgtgctcca tcttcgatcg ccaacaggtc gtagggaccg gccgacaaca cgtcgcgcac
     1321 gacacccaca agaatcccgg cctcggtctc gaccgacatc cccttgagtt cgtcgtggta
     1381 aaacgcacca tccggtaacg gggcgcgttc ggactcttcg atcgccagaa aacacccgcg
     1441 ccaggcactg accgcatcaa tcgactcccg gccctcgaag cgcagcacca accgtccatc
     1501 gggcgcgccg gccgcctccg caagggtcac ccggaccgga ggggtggaca gcacccaaaa
     1561 tcgagcgccc gcggtaaacc gatccggcgc gtccgacatc gcttccactc tgacggctcc
     1621 cttgagccca atgggcttca ccacccgggc gaccaccacc gggctcgctg gtccgacggc
     1681 gaccgcagcc ggaccgttct tgaacgcgac gctcgtcaca ccgcgcgcgg cgacgccgaa
     1741 ccgaccccgg aacgtttgaa caactggcgg actgtatccg tcacctgcgc gcctcgcgcc
     1801 aaccatccgt tggcccgctc cgcgttgacc cggaccgcgg cggacgccgc cagcgggtca
     1861 tatgtcccca gcacttcgat gaatcgtccg tcccgtggcg accgcgagtc cgccaccacg
     1921 atccgataca acggccgctt gtgacgaccc tgtcgcgcca accgaatttt taccatacgc
     1981 gttccatccc tttccggttc tctctgagcc ctggtctaca gcgaaccctt gagtgactgt
     2041 ttggcggcct tgagcaaccc ggcatacccg ttgatcgccg ccgtgccgac accatatgta
     2101 atcgagagcc cctccgcgac gctctgcgaa ggcaccttcg cgcgaatcct cgcgatcaaa
     2161 ctcgccgcgc cgcttgaatc cgcgtccaac aacacgacca cgaacgtgcc gtgatcatat
     2221 cgcccggcga tatcgacccg acgaagcgac gcacggatgg cctccgcgac tccgcgaatt
     2281 cgccggtccg catcgggccc ctcaagcgcg tcacccgaca gcgacacgac gaccgcggcg
     2341 agcgcgcgct gctgtcgttc ggccaaccgc acctctcgcc gatacaactc gtcgaagtag
     2401 gatctggcgt agaaccccga gacgcgatca acgcgctcga ccgacagaac gtgctcttcc
     2461 aacgccgcac gatccgaaac gtcgcgcacc aaaatcatcg ccagcgactc cccgccgacc
     2521 gacttgagcg gaacacacgc cgccggcgcc gtttgtccgc cgcggcccgg cagattcacc
     2581 tccagatcga ggcgtcgctc tcctcgggaa aacgccgctt gccccggaca cgccgtctcg
     2641 cacagttcaa atccctcggc gttctgacac accgccactt cgctacaaat cttgatgccg
     2701 accgccgcct tggcgggaca cgcggtcaac tgctcggcgg cggggttgat cgcgacgatg
     2761 cgtagcgagc tgtcgatcac gatcacgccg tcggaggcgt gccggaacag ttccgtgaac
     2821 cgctcctggt cgatctgaaa ccccaccggt cgctcctggg ttctccgcgg cgccacaaca
     2881 gccacgttat gcggaaaaca gtcgagccat cgcactacgc ccgcgggagg tcttgagcgt
     2941 ctt
//
LOCUS       DBNGTT010000371         5599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3409506, whole genome shotgun sequence.
ACCESSION   DBNGTT010000371 DBNGTT010000000
VERSION     DBNGTT010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3409506"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="AC16BC_08360"
     CDS             complement(<1..1136)
                     /locus_tag="AC16BC_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR3241674.1"
                     /translation="MNETPTTAIDPVCGMTVDTANPRGGTHVHEGKTYYFCNPSCRER
                     FKADPARYLDLAPPKPALMTIGPPVAKREAPATAIDPVCGMAVPIADPKGGTHVHEGT
                     TYYFCNPSCRDRFKAAPARYLASSEPHLMTIGVPPVQRPEPTPAAPPGAKLEYICPMD
                     PEVLSDRPGPCPICGMALEPRTVQVQEGPNPELIDMTRRFRVGLIVGFPVFALAMADL
                     VIGPAVRGTISMRVSNWIQLLLATPVVLWAGWPFFERGWTSLVTRRFNMFTLIALGVG
                     AAYVYSAAAAVAPGLFPEGFRVHGAVEPYFDTAVVITLLVLLGQVLELRARGRTGAAI
                     RALLGLAPKTARLVRDGREEDIPLERVRRDDLLRVRPGEKVPVDG"
     gene            1350..2024
                     /locus_tag="AC16BC_08365"
     CDS             1350..2024
                     /locus_tag="AC16BC_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HIR3241675.1"
                     /translation="MKTYQQAAEPVTIADDPKARELLRGAFESTYRWTADFKGFRARL
                     AVREAGKQTEGDVLVELPETVKVSLSDADAQKWAEGQIGMMAVHRGHRAFEQADGRHT
                     LTLGEIDGHPLGRLLLIHGDGMNSRYRILANQIRQINRSMGPARFTINVEDSTATTDG
                     RRLTTRYTVYYFNPQDGAIKQVDSITDVHAVVDGVYLPGTRRVIGAENGAVRVRMMEF
                     SGHQLI"
     gene            2039..2299
                     /locus_tag="AC16BC_08370"
     CDS             2039..2299
                     /locus_tag="AC16BC_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241676.1"
                     /translation="MRCTACDWTAVSFDLESRTWRCKRCHAAVTDSGGPGFAGSAKAG
                     APKKPAASRASTAGKAKPAAKKKSARAAAKPAPRKPAARKKR"
     gene            2296..2646
                     /gene="arsC"
                     /locus_tag="AC16BC_08375"
     CDS             2296..2646
                     /gene="arsC"
                     /locus_tag="AC16BC_08375"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033580.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIR3241677.1"
                     /translation="MSGVTIYHNPRCSTSRKTLDLIRAAGIEPTVIEYLKTPLSASEL
                     DRILTLLKMEPRDLMRKKEDEYKTLKLDRRDLTRKDLIKAMADHPILIERPIVLSKGK
                     AALGRPPENVAAIL"
     gene            complement(2703..3200)
                     /locus_tag="AC16BC_08380"
     CDS             complement(2703..3200)
                     /locus_tag="AC16BC_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR3241678.1"
                     /translation="MQGISLFFRWGHFLAGITWIGLLYYFNAVQVPFLKAVTPEAKAE
                     AFKHLVPRALWWFRWAALATWIFGAGLLGPRLVGAFTLQGPDAIIGMGAWLGTIMLFN
                     VWGIIWPNQKLVLGLKAAKPEEKAAAARKAFLASRVNVMLSIPMLFFMASSSHGTGLF
                     MQMGG"
     gene            3530..4519
                     /locus_tag="AC16BC_08385"
     CDS             3530..4519
                     /locus_tag="AC16BC_08385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3241679.1"
                     /translation="MTAPLTEHDASTQAASLIVEAEPIRPSGFAQRVAWALLRGIQFV
                     VTIMPLRAALWLGRRLGDAAYYAVYPRTRIALEQIAGAMPELSRSEQRATIRDMYRQF
                     AQGVVEFLRLPRTVATGQLDQLVAVDGEDIVRNAYEQGKGLLILTAHYGNFELLSTFF
                     AAHGYQVNLVTRRLKNGALDRFWAEQRRRLKIRAIFKEQSLKEVIARLRRNEAMGYVL
                     DQNMGLGQGVFVEFFGRPACTLGVVAVLAKRFGSPVIPVFIARDPHDPTRHRIVFEQP
                     VPFETNADDKSDADIVENTQRYTAIIERRVRERPDHWIWIHKRWKTRPPQKAG"
     gene            4524..5138
                     /locus_tag="AC16BC_08390"
     CDS             4524..5138
                     /locus_tag="AC16BC_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HIR3241680.1"
                     /translation="MAESLVPKHGGYRKLKSFQVAQLAYDVTVRFCDRYVEKRSRTHD
                     QMVQAARSGVQNIAEGSQASGTSKKMELKLTNVARASLEELRLDYEDFLRQRKLALRE
                     RQDSRRKNLIDRRPDTADAVAQWAREIHETSGRRGLSGRSGHDNRSTSSTYPEIAANG
                     ALALIAVATVLLDRQIAAQAKSFETEGGFTERLYRTRSAKRNER"
     gene            5227..>5599
                     /locus_tag="AC16BC_08395"
     CDS             5227..>5599
                     /locus_tag="AC16BC_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241681.1"
                     /translation="MIQVARRFVGTVHFFQGPSKSNPVALYARKQGADFLIAAVIEPW
                     NTVSGTGPTINKAAGNFEINWKAQPIPAEQYSGPHWEEGIKAEKPAPPPKPATPAPAA
                     AAGTAAAAPAQAAAAAPPAAES"
BASE COUNT         1100 a         1736 c         1864 g          899 t
ORIGIN      
        1 ccgtccaccg gaactttttc accgggtcgc acccgtaaga gatcatcgcg ccggacgcgt
       61 tcgaggggaa tgtcctcttc gcggccgtcg cggaccaggc gcgcggtttt gggcgcgaga
      121 cccagcaggg cgcgaatggc cgcgccggtg cggccgcgcg cgcgaagttc cagaacctgg
      181 cccagcagga ccaggagggt gatgaccacc gcggtatcga aatacggctc caccgcgccg
      241 tgcacgcgaa agccctcggg gaacagcccc ggcgcgacgg cggcggccgc gctgtagacg
      301 tacgccgcgc ccacgcccag cgcgatcaac gtgaacatgt tgaaccgtct tgtcacgagg
      361 gacgtccacc cgcgttcaaa gaacggccac cccgcccaga gcacgaccgg ggtagccagc
      421 agcaactgga tccagttcga gacgcgcatg gaaatcgtgc cgcgtacggc gggtccgatc
      481 accaggtcgg ccatggccag ggcgaacacg gggaacccga cgatcagccc gacccggaac
      541 cggcgggtca tatcgatcaa ctcagggttc ggtccctcct gaacctgcac ggtgcgcggc
      601 tccagcgcca tgccgcagat cgggcagggc cccgggcgat cgctcagcac ctcgggatcc
      661 atggggcaga tgtattcgag cttggcgccg ggcggcgccg ccggcgtcgg ttccggtcgt
      721 tgaaccggcg ggacgccgat ggtcatgagg tgaggttcgg acgacgccag gtagcgcgcc
      781 ggggcggcct tgaatcggtc gcggcaactt ggattgcaga agtagtacgt agtgccttcg
      841 tgaacgtgcg tgccgccctt ggggtcggcg atcggcacgg ccatgccgca gaccgggtcg
      901 atcgcggtcg ccggcgcctc gcgcttggcg accggcgggc cgatcgtcat cagcgcgggc
      961 ttgggcggcg cgaggtcgag gtagcgggcc ggatcggctt tgaaccgttc ccggcagctc
     1021 ggattgcaga aatagtaggt cttgccctcg tggacgtggg tgccgccgcg cgggttggcc
     1081 gtgtccacgg tcatgccgca gaccgggtcg attgcagtgg tcggcgtctc gttcatggat
     1141 gccatctcgc ggcggtaacc ggcagcctat cgcatcggcc acgcctaatc aaggcgactg
     1201 ggtttctttg cgacagaggt ctcggtgtcc gatgcgggag agcgggtcag gccacgaccg
     1261 tctgatcgcc gtcgggtcga tacgcggcct tgtaattttt cgcgcaactc gctaggctcc
     1321 cacctgctca ttcacaccag ggggacgcca tgaagaccta ccaacaggcc gccgaaccgg
     1381 tgaccattgc ggacgatccc aaggcgcgcg agctgctccg gggcgcgttc gagtccacgt
     1441 atcgctggac agccgacttc aaagggtttc gcgcacgcct cgcggttcgg gaagcgggga
     1501 aacagaccga gggcgacgtg ctggtcgagc tgccggagac ggtcaaggtt tcgctgtctg
     1561 acgccgacgc gcagaagtgg gccgagggac agatcggcat gatggccgtg caccgcggac
     1621 accgcgcgtt cgaacaggcc gacggccgcc acaccctcac gctcggcgag attgacgggc
     1681 atccgctggg tcgcctgttg ttgatccacg gcgacggcat gaactcgcgc taccggatcc
     1741 tggccaacca gattcggcag atcaaccgca gcatggggcc ggcgcgtttt accatcaacg
     1801 tggaagactc gaccgccacg accgacggcc gtcgtctgac cacgcgctac accgtctact
     1861 atttcaaccc gcaggacggg gcgatcaaac aggtggacag catcacggac gtgcacgcgg
     1921 tggtggacgg cgtgtatctc ccgggcacgc gtcgcgtcat cggcgcggag aacggcgcgg
     1981 tgcgcgtgcg catgatggag ttcagcgggc atcaactgat atagggagga gcgaggggat
     2041 gcggtgtacg gcatgcgact ggacggcggt gtcattcgat ctggaatcga gaacgtggcg
     2101 gtgcaaacgg tgtcacgccg cggtgactga ctcgggcggg ccggggttcg cgggctcggc
     2161 caaggcaggc gcgccgaaga aacccgcggc gtcgcgcgcc tcaaccgccg ggaaagccaa
     2221 acccgcggcg aagaagaaat cggctcgggc cgccgcgaaa ccggcgccac ggaagcccgc
     2281 ggctcggaag aaacgatgag tggcgtgacc atctatcaca acccgcgctg cagcacctcg
     2341 cgcaagacgc tggatctgat ccgggccgca ggcatcgagc ccaccgtcat cgaatacctc
     2401 aagaccccgt tgtccgcgtc ggaactggac cgcatcctca cgttgctcaa gatggaaccg
     2461 cgggacctga tgcggaagaa agaagacgag tacaagacgc tgaaactcga tcgccgggat
     2521 ctgacgcgca aggacttgat caaggcgatg gcggaccacc cgatcctgat cgagcggccc
     2581 atcgtgttgt ccaaaggtaa ggccgcgctg ggcaggccgc cggaaaacgt ggccgcgatc
     2641 ttataaaaaa cagggcgcag ggaccgcgag gatccctgcg ccctgttcac gctgttccga
     2701 aattacccgc ccatctgcat gaacaggccg gtcccgtgcg aggacgaggc catgaagaac
     2761 aacatcggaa tcgacagcat cacgttcacg cgcgaggcca ggaacgcctt gcgcgcggcc
     2821 gcggccttct cttccggctt ggccgccttc aaacccagga ccaatttctg gttcggccag
     2881 atgatgcccc acacgttgaa gagcatgatc gtgccgagcc atgcgcccat gccgatgatg
     2941 gcatccgggc cttgcagcgt gaaggcgccc accaggcggg ggccgagcaa cccggcgccg
     3001 aagatccagg tggccaacgc ggcccagcga aaccaccaca acgctctggg caccaagtgt
     3061 ttgaacgcct cggctttggc ttcgggcgtg acggctttca gaaacggtac ttgcacggca
     3121 ttaaagtagt acaggagtcc gatccacgta atcccggcca ggaagtggcc ccaacgaaag
     3181 aacagcgaga ttccttgcat gtcggcgccc tcctctcaga ccaacgcgtt gacgaagaaa
     3241 tacaggacca gcgtgagcgc aaatccgagg ccgatcgtgg ccggcagcga atcgagtgga
     3301 ttcgtcattc gaaattcctc ctgtgaggtc gtgggtgacg cgaagatccc gctcgcgtcc
     3361 atcgtggatc aatcgccggt atcatagtca ggggcatgat gtaagtcaac ggccgggcat
     3421 gatgccggtg ggtagcggtc ggcgaccgcc gatgccgtcc ggaaacgcga tgtgatatgc
     3481 tctgcccgcg ccggcccacc gtgatcgacg cgggagccgc aacaacacag tgaccgcacc
     3541 gctgactgaa cacgacgctt ccacgcaagc cgcttcgctc attgtcgagg cggaacccat
     3601 ccggccgtcc ggtttcgcgc agcgcgtggc gtgggcattg ctgcgcggca tccagttcgt
     3661 cgtcacgatc atgcccctgc gcgcggcgtt gtggttgggg cgacggttgg gcgacgcggc
     3721 ctattacgcg gtgtacccgc gaaccaggat cgccctggaa cagatcgcgg gcgccatgcc
     3781 cgagttgtcg cgatcggaac aacgcgcgac cattcgcgac atgtaccgcc agttcgcgca
     3841 gggcgtggtg gagttcctcc ggctgccgcg cacggtggcc accggacaac tcgatcaatt
     3901 ggtcgcggtg gacggggagg acatcgtgcg caatgcgtat gagcaaggca agggcctgct
     3961 cattctgacc gcgcactatg gcaacttcga attgctgtcc acgttttttg cggcccacgg
     4021 gtatcaggtc aacctggtca cgcgccggtt gaagaacggg gcgctcgatc gcttctgggc
     4081 ggagcaacgc cgccggctca agatccgcgc gatcttcaag gagcagtcgc ttaaagaggt
     4141 gatcgcccgg ctgcggcgca acgaggccat ggggtacgtc ctggatcaga acatggggct
     4201 tggtcagggc gtgttcgtcg agtttttcgg acggcccgcg tgtacgctcg gggtggtggc
     4261 ggtgctggcc aagcgattcg gcagcccggt gatcccggtg ttcatcgcgc gcgaccccca
     4321 cgaccccacg cggcaccgca tcgtgttcga gcagccggtt cccttcgaga caaacgccga
     4381 cgataagtcc gacgcggaca tcgtcgagaa tacccagcgt tacaccgcga tcatcgaacg
     4441 ccgcgtgcgc gagcgcccgg accactggat ctggattcac aagcgctgga agacccggcc
     4501 gccgcaaaag gcgggatagg gtcatggccg agtccttggt cccaaaacat ggcgggtacc
     4561 gcaaactcaa gagctttcag gtggcgcagc ttgcctatga tgtcacggtg cggttttgtg
     4621 accgatacgt cgagaaacgc agccggaccc acgatcaaat ggtgcaggcg gcgcggtccg
     4681 gggtgcaaaa cattgccgag ggcagccaag ccagcgggac ctcgaaaaag atggaactaa
     4741 aactgaccaa cgtggctcgg gccagcttgg aggaacttcg gttggattac gaagattttc
     4801 tgcgccagcg aaagctggcg ctgcgggaaa gacaagattc acgacgaaag aacctgattg
     4861 atcggcggcc ggacacggcc gatgccgtgg cgcaatgggc tcgggagatt catgagacta
     4921 gtggacgacg tggactgagt ggacgcagtg gacacgataa taggtccaca tcgtccacct
     4981 atcctgaaat tgccgccaat ggcgcgctgg cgctcatcgc cgtggccacg gtgcttctgg
     5041 accgacagat cgcggcgcaa gccaagagct ttgaaacaga aggcgggttc accgaacgcc
     5101 tgtaccggac gcgctccgca aagagaaacg aacggtaaga ttgctcgcag tcttgcattg
     5161 ccccgcgaca ttatggcacc atcccggcgt taggtggcgt ctgatcagca atagaaggag
     5221 cccgtcgtga ttcaagttgc gcgtcgtttc gtggggaccg tccatttttt tcagggaccg
     5281 tccaaaagta atccggtggc cttgtacgcc cgtaagcagg gcgcggactt tctcattgcg
     5341 gcggtgatcg agccgtggaa tacggtgtcg gggaccggcc cgaccatcaa caaggcggcc
     5401 ggcaatttcg agatcaattg gaaggcgcag ccgataccgg ctgaacagta ttcggggccg
     5461 cattgggaag aggggatcaa ggccgagaaa cccgcaccac cccccaagcc cgcgacgccg
     5521 gctccagccg cggcagcggg aacggccgcc gccgctccgg ctcaagcggc tgctgcggcg
     5581 cctccggcgg ccgagtcgc
//
LOCUS       DBNGTT010000372         6041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_856401, whole genome shotgun sequence.
ACCESSION   DBNGTT010000372 DBNGTT010000000
VERSION     DBNGTT010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_856401"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(253..486)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            492..1796
                     /locus_tag="AC16BC_08400"
     CDS             492..1796
                     /locus_tag="AC16BC_08400"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL and HDOD domain-containing protein"
                     /protein_id="HIR3241682.1"
                     /translation="MRRGVSSAVLTRVDAWSTWTMLYLSAVGDIVDVFIARQPIFDRQ
                     QQVYGYELLFRSGWDNVFRHHDLNQASAQVIANSLLVMGLDTMTSGRMSFVNCTRDAL
                     VKQYASVLPSRTTVVEVLEHVDADDEVVAACKSLKAAGYTLALDDFVFRDSVEPLLAL
                     ADIIKVDVLNSSVDEQRELFDRCGGRIPMLAEKVETREEFDRGLAMGYRYFQGYFFSK
                     PVVLNGRHIPTNKLSVLELLGEINRSDLDYTKLERILQREVSLSYKLLRYINSAAFAF
                     RSQIGSLKQAMVLLGEREVKKFASLIALADMGHDKPGELLVTAVVRGRFGEVLAPKLA
                     MGDRGPDLFLTGLFSVIDALMDRALPDLLDSLPLEDEIKQALMGRNSRFQFVHQLIMA
                     YERGDWKEVARLSQAAHLGDTDLQSCYLAAVDWAQQTVRQAL"
     gene            complement(2046..2195)
                     /locus_tag="AC16BC_08405"
     CDS             complement(2046..2195)
                     /locus_tag="AC16BC_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241683.1"
                     /translation="MLAFPVQRGYLEHPQNVTPAGAPQGIRTTIVLPHFGQPGDAVAP
                     NFPSP"
     gene            complement(2199..2393)
                     /locus_tag="AC16BC_08410"
     CDS             complement(2199..2393)
                     /locus_tag="AC16BC_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241684.1"
                     /translation="MNLATVVDDALKLLRATLPTTIEIRTEFPAALPTVSENPAQIHQ
                     IIMNLSTGPTSSDRMDSPGS"
     gene            2706..5492
                     /locus_tag="AC16BC_08415"
     CDS             2706..5492
                     /locus_tag="AC16BC_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3241685.1"
                     /translation="MDVTQTAHALLETLEALAIELHQTPPGRALTLDSSLERDFGFDS
                     LGRVELVMRLERRFGVRLSEQVMVTAETPRELLRAVLNREEVPEPAVPVETVHARVGA
                     VAGDPGETATLIEALRWHVDRHPERPHIDLLDDRGRKDRITYADLWQGASAVAAGLRA
                     RGIGSGDTVAIMLPTSREYFTGFAGILLAGAIPVPLYPPLRLAQIEDHLRRQTAILRN
                     AGAVLMITVPEAQRVVHLLKAQVDSLRHAATVEELSAGPAETVRPAVNARDIALLQYT
                     SGSTGTPKGVILTHANLLANIRAMGEAVHASSEDVLVSWLPLYHDMGLIGAWLGSLYY
                     AAPFVVMSPLTFLSRPERWLRAITEYRGTISAGPNFAFELCVRKIDDAALASLDLSSW
                     RLAVNGAEPISPDTMSRFIDRFAACGFRPATMAPVYGLAESSVGLAFPPLGRVPPIDR
                     VDRDAFASQGVARPAAADDPNALRMVACGRPLPGHEIRVVDEDGREAADREVGRIQFK
                     GPSTTSGYYRNPDATKALFDGTWLESGDYGYLADGDIHITGRAKEVIIRAGRNLYPYE
                     LEEAVGAIPGIRKGCVAVFGLTDPANGTERLVVVAETRERDAPLLDALRARIHGLAMD
                     LLHTAPDDIVLAPPHTVLKTSSGKIRRSACSDLYRRGALGASGQRAVWLQVARVAAGG
                     ALVSARRWMRTAGRALYAAYAWTVFGAGMLTALVVVTVVPPAEARWRMVSAIARLGFR
                     LAGVPLVVRGHAPRGPVVFVVNHASYLDAVALAAALPAGAAFVAKRELAAAAISGFFL
                     RRLGTELVERFDRQKSVEDTGRMADVSRQGRSLVMFPEGTFGRAAGLRPFRMGAFVVA
                     AQTGTPVVPVAIRGTRSILRDGSWFPRRGPLSMVIGDPIAPQGGDWAAAIALRDAARA
                     EILRWCGEPDLKGC"
BASE COUNT          954 a         1900 c         2104 g         1083 t
ORIGIN      
        1 gtcacggccg gagagagcga aacgatgcgc cggggcgcgc ggtcgaattg cagcgtctgt
       61 cccaggtcgt cgcggaccac gagcggttcg gcttccgccg cggcgacgga ggtgatgagg
      121 gctacgacga cgaggcggag gaacctaggt cgtgatgaag cgtagggcac gcgcaatcct
      181 cgcgaaacac gcttcgagga ttgcacccag cttcttcatc ccgaagagcg gacaggcctt
      241 cagacgaggc gcgaccgcat gcggctccag gcaggtcttc tgactcccgg ctcatccgat
      301 tccctcggtc cttcccagcg cctgaggcgc cagtggactt gttgaacggt ttgaacgttt
      361 gaactcttga acgttcgtgc cgttccagag gggctcgtcc ccggttacag cggcgggacc
      421 gtcccggatt tccaccgggt tcccttttca cgcccgtgat gggcgacctg gatcgcgagc
      481 actgtagagg aatgcggcgg ggcgtgtcaa gcgcggtgtt gacgcgtgtt gacgcgtggt
      541 caacctggac tatgctctac ctgtcggccg tcggggacat cgtggacgtt tttatcgcgc
      601 ggcaacccat ctttgatcgt cagcagcagg tgtacgggta cgagctgttg ttccgatcag
      661 ggtgggacaa tgtgtttcgc caccacgacc tgaaccaagc ctccgcgcag gtcatcgcga
      721 acagcctgtt ggtgatgggg ctggacacca tgacgtccgg ccgcatgtcg ttcgtgaact
      781 gcacgcgcga cgccctggtc aagcaatacg cgtccgtgtt gcccagccgg accacggtgg
      841 ttgaagtgct ggagcacgtg gatgccgacg acgaggtcgt ggccgcgtgc aaatcgctca
      901 aagccgcggg gtacactctg gcgctggatg acttcgtgtt ccgcgactcc gtcgagccgc
      961 tcctggcgct ggcggacatc atcaaggtgg atgtcctgaa ctcgagcgtg gacgaacagc
     1021 gcgaattgtt cgatcgctgc ggcggcagga ttccgatgtt ggccgaaaaa gtggagaccc
     1081 gcgaagagtt cgatcgcggc ctggccatgg ggtaccggta ctttcaaggg tatttcttca
     1141 gcaaacccgt ggtgctcaac ggccgccaca ttcccaccaa taagctgagc gtgttggaac
     1201 tcctgggcga gatcaatcga tcggacctcg actatacgaa acttgagcgc attctccagc
     1261 gggaggtctc gctctcatac aagttgctgc gctacatcaa ttccgccgcg ttcgcgttcc
     1321 gctcccagat cggatcgctc aagcaggcga tggtgttgtt gggcgagcgc gaggtcaaga
     1381 agttcgcgtc gctcattgcc ttggccgaca tggggcacga caaacccggt gaactgttgg
     1441 tcaccgcggt ggtgcgcggc cgtttcgggg aggtgctcgc gcccaagctc gccatgggcg
     1501 accggggccc ggatctgttc ctcacggggt tgttttcggt gatcgacgcg ttgatggatc
     1561 gggccttgcc cgatctgctc gactcattgc cgctggagga cgagatcaag caggcgctca
     1621 tgggccgcaa cagccggttt cagttcgtgc accaactgat catggcgtac gagcggggag
     1681 attggaaaga agtggcgcgc ctgagccagg ccgcgcatct cggcgatacc gacctgcaat
     1741 cctgctatct cgccgcggtg gactgggcgc agcagacggt ccgacaagcc ttgtagtgcc
     1801 ctcgtcagtc ccgcaaacgc gggaatccgg ttccgcccca actttgtgga tgagtcgacg
     1861 gttttgtcgc cgtgccgctt ccgtggcgcg gcgatgatgg gttccttggg cggtggcgtg
     1921 gattgtgcaa taaataacca tgcaatcgag ggatcagggg ccaaggaggg ttcagggatg
     1981 gcaccgagac gacataacgc aatggccgga catgcaacgg atgcggctcc cgtggtcggg
     2041 atggctcacg gcgatggaaa attcggcgcc accgcgtcgc cgggctgccc gaaatgcggc
     2101 aacacgatgg tcgtgcgaat cccctggggc gcgccagcgg gcgtcacgtt ctgggggtgt
     2161 tccagatatc cccgctgcac ggggaaagcc agcacggtct aggaacccgg cgagtccatt
     2221 cgatcggacg atgtcggtcc ggtcgaaaga ttcatgatga tctggtggat ctgcgccggg
     2281 ttctccgaca ccgtggggag cgcggccgga aactcggtgc ggatctcgat ggtcgtgggg
     2341 agcgtcgcgc gcagcagttt gagcgcgtcg tccaccaccg tggccagatt caccacctcg
     2401 tattcgggtg tcctgcgggc ggctgaagag caggatgcgc cggaccagtt cggccgcgcg
     2461 cgccggcctg gtcgatttcg cgcaggtgcc ggtccgcctg gcgtcgcgag gacgagtcga
     2521 tttcgccggc ccgcctcgtc gttgttgcct cagatcatgg aaccagcccc atggaacaac
     2581 acgacgaagg acgcccatcg ataggcctgt tgcgattccc aagtcaagtc gggccgacac
     2641 ggcagggcga tctcggctga cgcggcgtgc aggatggcgt ccgattagtg taggctgacg
     2701 cccacatgga cgtgacgcag accgcacacg cgttgctcga gacgctggaa gccctggcga
     2761 tcgaactgca tcaaaccccg ccgggtcgcg cgttgacgct cgacagttcg ctggagcggg
     2821 atttcgggtt cgacagtctc ggacgggtgg aactcgtgat gcggttggag cggcgcttcg
     2881 gggtccggct gtccgagcag gtgatggtca ccgcggaaac cccccgcgag ttgctgcgcg
     2941 cggtgttgaa ccgcgaggaa gtccccgaac cggcggtccc ggtggagacc gtgcacgcgc
     3001 gcgtgggcgc ggtcgcgggg gatccgggtg agaccgcgac attgatcgag gccctgcggt
     3061 ggcacgtgga ccgccacccc gagcggccgc atatcgacct gctggatgat cggggccgca
     3121 aggaccggat cacgtacgcg gacctgtggc agggcgcctc ggcggtggcc gcgggtctgc
     3181 gcgcgcgggg catcgggtcc ggcgatacgg tggcgatcat gctgcccacc tctcgcgagt
     3241 acttcacggg cttcgcgggg atcttgctgg cgggcgccat cccggtgccg ctctatccgc
     3301 cgttgcggct ggcccagatc gaagaccacc tgcggcgcca gaccgcgatc ctgcgcaacg
     3361 cgggcgcggt gctgatgatc accgtgcccg aggcgcagcg cgtggtgcac ttgctcaagg
     3421 cccaggtgga ttccctgcgg cacgcggcca cggtggagga gttgtccgcg ggtccggcgg
     3481 agaccgtgcg tcccgcggtg aatgcccggg acatcgcatt gctgcaatac acctccggga
     3541 gcacgggcac gcccaagggc gtcatcctca cgcacgccaa tctcctggcc aatatccggg
     3601 ccatgggcga ggcggtgcat gcgagctccg aggatgtgct ggtgagctgg ctcccgctgt
     3661 atcacgacat gggcttgatc ggggcgtggc tcgggagtct gtattacgcc gcgccgttcg
     3721 tggtgatgtc gccgctgacg ttcctgtcgc ggcccgaacg ctggttgcgc gcgatcaccg
     3781 agtaccgcgg cacgatctcg gcgggtccga acttcgcgtt cgaattgtgc gtgcgaaaaa
     3841 tcgacgacgc cgcgctcgcg agcctggatc tgagttcctg gcgcctggcg gtcaacggcg
     3901 ccgaacccat cagcccggat accatgtcgc ggtttatcga ccgattcgcg gcctgcggat
     3961 tccgccccgc caccatggcc ccggtctacg ggctcgccga atcgtcggtg ggtctggcgt
     4021 tcccgccgct cggacgcgtg ccgccgatcg atcgcgtgga tcgcgacgcg ttcgcgtccc
     4081 aaggcgtggc ccggcccgcg gccgcggacg acccgaacgc gttgcgaatg gtggcgtgcg
     4141 gcaggcccct gccggggcat gaaatccgcg tggtggacga agacggccgc gaggcggctg
     4201 accgcgaggt ggggcggatc cagttcaagg ggccgtccac caccagcggc tactaccgca
     4261 atcccgacgc caccaaggcg ttgttcgacg gaacgtggtt ggagtcgggc gactacgggt
     4321 acctcgcgga cggggacatc cacatcacgg ggcgggccaa ggaagtgatc attcgcgcgg
     4381 gcaggaacct ctatccgtac gaactggagg aggcggtcgg cgcgattccc ggcatccgca
     4441 aaggctgcgt ggcggtgttc ggtctcacgg acccggcgaa cggtaccgag cggttggtgg
     4501 ttgtggccga aacccgggaa cgcgacgcgc ccttgctgga cgcgctgcgc gcccgcatcc
     4561 acgggttggc catggatctg ctgcacaccg cgccggacga catcgtgttg gccccgccgc
     4621 acacggtctt gaagacctcg agcggcaaga tccggcgttc cgcctgcagc gacttgtacc
     4681 ggcgcggcgc gctgggcgcg tccgggcagc gggcggtgtg gctgcaagtc gcgcgcgtgg
     4741 ccgcgggcgg cgcgctcgtg agcgcccggc ggtggatgcg caccgcgggc cgcgcgctct
     4801 atgccgccta tgcctggacc gtattcggag cgggcatgct gacggcgttg gtggtggtca
     4861 ccgtcgtccc gccggcggag gcgcgttggc gcatggtttc cgcgatcgcg cgcctcggct
     4921 ttcgtctggc cggcgtgccc ctggtcgtgc gcggccatgc gccccgcggt ccggtcgtgt
     4981 tcgtggtcaa ccacgcgagt tacctggacg cggtcgcgct tgccgccgcc ctgccggccg
     5041 gcgccgcgtt cgtggccaaa cgggaattgg ccgccgcggc gatcagcggg ttttttctgc
     5101 gacggcttgg caccgaactg gtcgagcggt tcgaccgtca gaagagcgtc gaggacaccg
     5161 gacgcatggc cgatgtgtcg cgacaagggc gttccctggt gatgtttccg gaaggcacgt
     5221 tcggccgcgc ggccgggctg cggccgttcc ggatgggcgc gttcgtggtg gcggcgcaga
     5281 ccggcacccc ggtggtcccg gtggcgatcc gcggcacccg ctcgattctg cgcgacgggt
     5341 cgtggtttcc tcgccgcggc ccgctcagca tggtcatcgg cgatcccatc gccccgcaag
     5401 gcggggactg ggccgcggcc atcgcgctgc gcgacgcggc gcgcgcggag attctgcgtt
     5461 ggtgcggcga acccgatctg aaaggctgct gaaaaagtcc ggctgctgcg ttgaggtctg
     5521 aggagtcccg gccgcctcac cgactcggcg gcgctcaagg cttggcgcca cttattcgtg
     5581 gcgccactcg ccccacgtac gacacagtac gctccggttt tcctcctccg aggccttgcg
     5641 acccaccacc aacaactcaa atcccggaag tccccgaagg gggcatccgg agctttttga
     5701 gcagccttcg gcttcgccgg acttcctcgg atctgcgcag ataggacccc gagagaacta
     5761 ccgcatctgc gttacgacga cgtcggcttg tccgatgccg gtttggacac ctggagcctt
     5821 cggtggtcgg atagtacgtc ggtcatttcc agttgcccgc gaagcgctcg ttgcggtata
     5881 cacctgctag accatgacgg ccttcaccta caccccgatc ggcgtgtttc ggaccccgta
     5941 ccggaccacc gacgagatcc ccaagtcatt gggccaggct ccgagcgcgg agggcgtgat
     6001 cgaggtcgat cccgcgtacg cggacgggct gctcgacatc g
//
LOCUS       DBNGTT010000373         2580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1622724, whole genome shotgun sequence.
ACCESSION   DBNGTT010000373 DBNGTT010000000
VERSION     DBNGTT010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1622724"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..378)
                     /locus_tag="AC16BC_08420"
     tRNA            complement(302..378)
                     /locus_tag="AC16BC_08420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Arg,seq:cct)
     gene            553..960
                     /locus_tag="AC16BC_08425"
     CDS             553..960
                     /locus_tag="AC16BC_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3241686.1"
                     /translation="MRTQGAVKTEATVADYMTQEVRSVRWDATLKDAGKLLQKYKIGL
                     LFVDDDKRYVGVITESGLSRNAVAKGLDPNTTLVKTVMTRKIVSIEDNEPIIEAVKLM
                     KEHGIRHLAVTVNETVVGIISISDIVRYYSGVS"
     gene            complement(976..1356)
                     /locus_tag="AC16BC_08430"
     CDS             complement(976..1356)
                     /locus_tag="AC16BC_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241687.1"
                     /translation="MAQQLNLRVEVGVGKVLFHVRMTRHEDFVKPIEKSALVEAAGVT
                     HFDRTKRLRIDGDLFVQFACGFSSHRPPRPGGNVPVSSIEYRRAHAIGNADRSSNAGR
                     AGASEPRSLSDTRSADRLEGKTAL"
     gene            complement(1694..>2580)
                     /locus_tag="AC16BC_08435"
     CDS             complement(1694..>2580)
                     /locus_tag="AC16BC_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241688.1"
                     /translation="GKAQCPLCHAFIAGSLDDRAPNLLGIAARAGQRVKEARYLQPDS
                     VQTESFPGSGRATTAVEYIAESHACPSCYVVVGYGVRGTNDRVSPMAVIHKPPIGLTI
                     DEQIAVDTFLFYREGGDVPPVAEIKAAYERFIPPGEREQPKHVERGPDNGILPTDTPR
                     DMIAKLACAACHKIPTTIATVGVIGPMLIEKTNAPKRLASREYRALVKAGMAHASTPK
                     EYVMESIVNPNAFIVPGYGLPTTPPTSPMPHGFGQKLSYDALDRLADFLLSIDEAAAV
                     KAGMLTGPAAPASLASAS"
BASE COUNT          516 a          810 c          766 g          488 t
ORIGIN      
        1 ccttcaccac tcccgtaccg atacctcgct agtgtttcac aggcgcacgc tgctagagcc
       61 gccacggcga agttccctcc tgggtcgagg ctcagaattc gtgtgagatc gcgctcgata
      121 aagccgaaga ccctatcaaa cttcttttcg atgtcggcga aggacgcggt catgggagac
      181 tcctttatcc cctgtcagtg tctgttggga gaattgatgg gactgtttcc cggaattcgg
      241 tcatagttta ggcatagttg gcgtgctctc gcgaagtgcc gttctgcaac tcattgaaaa
      301 atggtgcccc cgggccgaat tgaacggccg acacgcggtt taggaaaccg ctgctctatc
      361 caactgagct acgggggcgt ctgcgcacgg cgccgcaaac cggcagggag ttatatcaca
      421 aggcactagg cgtgtcatta cgtgcgacgc gccgccgctc ctctgccatc ctttccggcg
      481 atttggtaca cttcttccca cgaattcatc cgggtgcgcg gcacccaata aactcttagc
      541 acgaggagcg tgatgaggac acagggagcg gtgaagactg aggccacggt ggccgattac
      601 atgacgcagg aagtgcgcag cgtacggtgg gacgccaccc tcaaggacgc gggcaaactg
      661 ctccagaaat acaagatcgg cctcctcttc gtggacgacg acaagcggta tgtcggcgtg
      721 atcacggaat cggggctcag ccgcaacgcg gtggccaagg ggctggatcc caataccaca
      781 ttggtcaaaa cggtcatgac ccgcaagatc gtctcgatcg aggacaacga accgatcatc
      841 gaggcggtca aactcatgaa agagcacggc atccgccacc tggcggtcac ggtaaacgaa
      901 accgtggtcg ggatcatctc gatctccgac atcgtgcgct actactccgg cgtctcctga
      961 cgccgtcccc gcaagtcaca acgcggtttt tccttcgagc ctgtcagccg atcgtgtgtc
     1021 actgagcgac cggggctcac tcgctccggc gcgccccgcg ttcgatgaac ggtcggcgtt
     1081 gccaatcgcg tgagctcgtc tatactcgat actcgacacc ggaacattcc caccgggacg
     1141 aggaggccta tggctgctga acccgcacgc aaactggacg aacaggtcgc catcaatccg
     1201 aagacgcttc gtgcggtcga agtgagttac gcccgctgcc tcaacaagag cggatttctc
     1261 aatcggtttt acgaaatctt catggcgagt catccggacg tgaaaaagca ctttgcccac
     1321 accgacttcg acgcgcagat tcagttgctg cgccacgggc tcatgtccgt cttcatgttc
     1381 tcggagaaca acccgatcgg gcaaaaaagc cctcacccgc atccgcgaat cccacaaacg
     1441 gaccaaactc aacatcgctc cgcccctcta ccagcattgg gccgacagct tgatcaaaac
     1501 cgtgtccgag ttcgacggcc agttcaacgc ggacctcgaa cggtcctggc ggcaggtcat
     1561 ccagcccgcc atcgactaca tccggtccgg gtacaacggc tgacccggcg ggcccgccac
     1621 cggcgcgcga tcagacatcg gggccgtctc tgtgttcatg catcagagac ggccccgagt
     1681 gttgatatcc gattcaactc gccgaggcaa gagacgcggg agcggcggga ccggtgagca
     1741 tgccggcctt gaccgcggcc gcctcatcga tcgagagcag gaagtctgca aggcggtcga
     1801 gcgcgtcgta actcaatttc tgtccgaacc cgtgcggcat gggagacgtc ggcggcgtgg
     1861 tgggcagacc gtatcccggc acgatgaacg cgttcggatt cacgatcgat tccatcacgt
     1921 actccttggg cgtcgaggcg tgggccatgc cggccttcac cagcgcccga tactcccgcg
     1981 acgcgagccg tttgggcgcg ttcgtctttt cgatcagcat cggcccgatc acacccacgg
     2041 tggcgatcgt ggtcggaatc ttgtggcacg cggcacacgc cagcttcgcg atcatgtccc
     2101 ggggcgtgtc ggtgggcagg atgccgttgt caggtccgcg ctccacgtgc ttgggctgct
     2161 cgcgttcgcc cgggggaatg aatcgctcgt acgcagcctt gatttcggcg actggaggaa
     2221 cgtcgccgcc ctcccggtaa aaaaggaagg tgtcgaccgc aatctgttcg tcgatggtca
     2281 agccgatggg aggcttgtga atcacggcca tcgggctgac ccgatcattc gtgcctctga
     2341 cgccgtaccc gacgaccacg taacaactcg gacacgcgtg cgactccgcg atgtactcca
     2401 ccgccgtggt cgcgcggccg ctgcccggga aggactcggt ctgaaccgag tccggttgga
     2461 gatagcgcgc ttccttgacg cgttgccctg cgcgcgcggc gatgcccagg agattcggcg
     2521 cccgatcgtc gagagatccc gcgataaagg catggcagag cgggcactgt gccttgccta
//
LOCUS       DBNGTT010000374         2755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1750844, whole genome shotgun sequence.
ACCESSION   DBNGTT010000374 DBNGTT010000000
VERSION     DBNGTT010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1750844"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="AC16BC_08440"
     CDS             <1..1111
                     /locus_tag="AC16BC_08440"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR3241689.1"
                     /translation="DTVDWVNTLFLIGTLVVAVIGVPIHLATIGFSWPILGLFAFYMA
                     ATGLSITGGYHRLFSHQSYQTTAPVRAFYLIFGAAACQNSALKWSSDHRYHHLYVDQD
                     RDPYNIGRGFFWAHIGWIFFKRSAYDFSNVQDLKKDPLVVWQNRYYVPLAIGVGGVLP
                     LLFGWAVGDWWGGFLLIGVARTVIVHHSTFLINSLCHYLGRQPYSTKDSSRDSGLVAL
                     LTYGEGYHNFHHHFQYDYRNGIRWFDWDPTKWLINTLAAVGWADRLRTAKDAHIFQAR
                     LEVQRETAKQKLAHRPQEFRSAMENKLHAAHDALVAARAKWEHLRVEYSAARQSMDTK
                     RRQLAATIKQELAAARLHFQNTHASWNLLLEAAGA"
     gene            complement(1150..1887)
                     /locus_tag="AC16BC_08445"
     CDS             complement(1150..1887)
                     /locus_tag="AC16BC_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR3241690.1"
                     /translation="MSTRAEILIIGNEILSGKVADEHAAFLCREFRALGVDVRRVVVL
                     PDDVDVIAEAVRAAWARADLIVTTGGVGPTHDDVTIEGVAKGLGRPLVRHPVLFGLVQ
                     ELYGASGDPYVNRLADVPEGATLITAEGLRVPVVSVDKLYVFPGVPEVFRRKFNAIKS
                     RFRSSPFHLRTIYLGVGEESIASLLYDAAARYPDLALGSYPVVGRNSYRVRLTLESKD
                     PTCLESAFAFLTERLPNGSVVAVDREP"
     gene            2059..>2755
                     /locus_tag="AC16BC_08450"
     CDS             2059..>2755
                     /locus_tag="AC16BC_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241691.1"
                     /translation="MAARRSIRFRPAGVCHAAIVTLTLAACGTAQTTLPVSSRLDAPQ
                     HVVAQSDGLGITVSWDPVDGASSYRVYWKQGSTVTPQSGSQATASAPPYFQQAAAFDL
                     TYAFVVAAVDDNGRTGRVSQVVTTTPSGAVFDAPQHVNAAAGDRLVIITWDPSPGATG
                     YRIEVTTPFGQFSVPDPVAPPFTHSPLLNRTEYGYRVRAKFGTQLGPWSVTVSAIPMP
                     AVPGSPIFTDVRGI"
BASE COUNT          495 a          944 c          832 g          484 t
ORIGIN      
        1 ggataccgtt gactgggtca acaccctgtt cttgatcggg acgctggtcg tcgcggtgat
       61 cggggtgccc attcatctcg cgaccatcgg gttttcgtgg ccgattctgg gtctcttcgc
      121 cttctacatg gcggccaccg gcctctccat caccggggga taccaccggc tgttctccca
      181 ccagtcgtat caaacgaccg cgccggtccg ggcgttttat ctgatcttcg gagcggcggc
      241 ctgtcagaac tcggcgctca agtggtcgtc cgaccaccgc taccaccacc tctacgtgga
      301 ccaggaccgg gacccgtaca acatcggccg cggatttttc tgggcgcaca tcgggtggat
      361 cttcttcaaa cgatccgcgt acgatttcag caacgtccag gacctgaaga aagacccgtt
      421 ggtggtgtgg caaaaccgct actacgtgcc gctcgccatc ggcgtcgggg gcgtcctgcc
      481 cctgctgttc ggatgggccg tgggcgattg gtggggcggg ttcctcctga tcggcgtggc
      541 gcggaccgtg atcgtccacc actcgacgtt cctgatcaac tccctgtgtc actatctggg
      601 ccggcaaccg tattcgacca aggactcgtc acgcgacagc gggctcgtcg cgctgctgac
      661 ctacggcgag ggttaccaca atttccacca tcactttcaa tacgactacc gcaacggcat
      721 caggtggttc gactgggacc cgaccaagtg gttgatcaac acgctggccg cggtcggctg
      781 ggcggaccga ctccgtaccg ccaaggacgc gcacatcttc caggcccgcc ttgaagtgca
      841 gcgggaaacg gcgaaacaga aactcgccca ccgtccacaa gagttccgca gcgcgatgga
      901 gaacaagctg cacgcggcgc acgatgcgct ggtcgcggcc cgcgcgaaat gggaacacct
      961 ccgggtcgaa tactcggcgg cccggcaatc catggatacc aaacgccggc agttggcggc
     1021 caccatcaaa caagaactcg ccgccgcgcg cctccatttc cagaacaccc acgcctcgtg
     1081 gaatctcctg ctggaggccg cgggagcata acggtcgttc gggagggacg agcctcctcg
     1141 tccctccctt tacggttctc ggtccaccgc caccacgctg ccgttcggca gccgctccgt
     1201 caggaacgcg aacgccgatt ccagacacgt cgggtctttt gattccaacg tcagcctgac
     1261 ccgatacgag ttccggccaa cgaccgggta cgagcccaac gccaaatccg gatatcgcgc
     1321 cgccgcgtca tataacaggg acgcgatcga ctcctccccc accccgaggt atatcgtgcg
     1381 caagtgaaac ggggaactcc gaaatctcga tttaatggca ttgaacttcc gccgaaagac
     1441 ctccggcacg ccggggaaca catagagctt gtcgaccgag acgacgggaa cgcgcaaccc
     1501 ttcggccgtg atgagggtgg ccccctccgg cacgtccgcc aatctgttga cgtacggatc
     1561 accggacgcg ccgtagagct cctgaaccaa accaaagagc accggatgac ggacgagcgg
     1621 acgccccaac cctttggcga cgccctcgat cgtgacgtcg tcatgggtcg gcccgacccc
     1681 gcccgtggtc acgatcaagt cggcgcgagc ccacgcggca cgcaccgcct cggcgatgac
     1741 atcgacatcg tccggcaaca ccacgacccg ccgaacatcc acccccaacg cccggaactc
     1801 tcggcagaga aacgcggcat gttcatccgc gactttgccg gagagaatct cattgccaat
     1861 gatcaaaatt tcggcgcggg tgctcatggg actgggatgc gttcgccggg tgacgccggg
     1921 cggtcgtcgt ccgtcggctg cgcgagtcta ccaccggacg ctcgggcccg acaagcgctg
     1981 tcgatgggcg ggggccggtc cgttatgctc ccgccggcgc ctggtgcgtt cgccaccggt
     2041 tccgtgctag catggcacat ggcggctcgg cgctccatcc gcttccgacc ggccggcgtc
     2101 tgccacgccg caattgtcac cctgacgctg gccgcgtgcg ggactgcgca aacgaccctg
     2161 cccgtgtcgt cgcgattgga cgcgccgcaa catgtcgtcg cccagtccga cggactcggc
     2221 atcacggtgt cgtgggaccc ggtcgacggc gcgtcgtcct atcgcgtgta ctggaaacag
     2281 gggtcgaccg tgactccgca atccggctca caggccactg cgtcggcgcc gccgtatttc
     2341 cagcaagcgg ccgcgttcga cctgacgtat gcgtttgtgg tcgcggcggt ggacgacaac
     2401 gggcggaccg gccgcgtctc ccaagtagtg accacgacgc cgagcggcgc ggtgttcgac
     2461 gcgccgcaac acgtcaacgc cgcggcgggc gatcgactcg tgatcatcac ctgggatccg
     2521 agtcccggcg ccacgggtta tcggatcgag gtgaccacgc cgttcggaca gttctccgtt
     2581 cccgatccgg tcgctccgcc gtttacccac tccccgctgc tcaaccggac ggaatacgga
     2641 tatcgcgtgc gggcgaagtt cgggactcaa ctgggaccgt ggtccgtaac ggtgtcggcg
     2701 atccccatgc cggctgttcc cggaagtccc attttcaccg acgtgagggg gatta
//
LOCUS       DBNGTT010000375        13381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1367622, whole genome shotgun sequence.
ACCESSION   DBNGTT010000375 DBNGTT010000000
VERSION     DBNGTT010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 13381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13381
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1367622"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="AC16BC_08455"
     CDS             complement(<1..900)
                     /locus_tag="AC16BC_08455"
                     /inference="COORDINATES: protein motif:HMM:NF017770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HIR3241692.1"
                     /translation="MLDSMRCSKCRLIQLAAPTCQSCGTPSPRLVIRQASASAPAARR
                     PVAEAPRPTAPPAPPATPHPAAASSPAPFDAEGDRTELVDKPTRASAAVTAVSEVVGD
                     ASTERRLTFEGQGGALFGIQIVNVFLTLLTLGIYSFWGKVRVRSYLMSQTEFEGDRFA
                     YHGTGKELYRGAVKAGLVFGGLSALLNASQVMPKGAPQISAVLAAYLGLSLLMPVAIV
                     GARRYRLSRTSWRNIRFAFRGNTGAFIKLFLKGSLLTGLTFGLYYPFFATNQHAFLIS
                     NSYFGDKSFGFNGRGRHLFGPYAL"
     gene            complement(1153..1518)
                     /gene="rplT"
                     /locus_tag="AC16BC_08460"
     CDS             complement(1153..1518)
                     /gene="rplT"
                     /locus_tag="AC16BC_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3241693.1"
                     /translation="MPRVKGGPKTKDRRRKRLKLAKGAYGGKSRLFRTATELVDKGLQ
                     YAFRDRRARKRDFRRLWIARISAAVRAEGLSYSQFMAALKNLGITLNRKVLSDLAIAD
                     PTGFSKVVSQAKTKTAAAA"
     gene            complement(1559..1780)
                     /gene="rpmI"
                     /locus_tag="AC16BC_08465"
     CDS             complement(1559..1780)
                     /gene="rpmI"
                     /locus_tag="AC16BC_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR3241694.1"
                     /translation="MPRTGKKMKTHKGAFKRFRTTGSGKISRKRAGNRHLLTHKNAQR
                     KRGYSTSVVLEPSEQSMIKRLLPYGTGKT"
     gene            complement(1832..2344)
                     /gene="infC"
                     /locus_tag="AC16BC_08470"
     CDS             complement(1832..2344)
                     /gene="infC"
                     /locus_tag="AC16BC_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR3241695.1"
                     /translation="MAVKHRVNQEIRIREVRVIGPNSEQIGVLPIGEALKRAEEAGLD
                     LVEVSPTAVPPVCRIIDYGKYRYEQSKKQQGAKQKSAQLKEVKLRPFTDTHDLDFKTR
                     HARDFLAEGHKVKITVMFRGREMAHQDAGRTILAKVIEQLGAAAQVEQQPRMEGRNMS
                     ILLAPKHGGG"
     gene            complement(2454..4412)
                     /gene="thrS"
                     /locus_tag="AC16BC_08475"
     CDS             complement(2454..4412)
                     /gene="thrS"
                     /locus_tag="AC16BC_08475"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR3241696.1"
                     /translation="MPSEIVLTFPDQTSRSFPAGVTPQAVIDSLDSARRARTIALKVN
                     GHVADLHAPLRHDARVEPLSFDSAEGREVFRHSSAHLMAQAVKRLFPGIKLAIGPAIE
                     DGFYYDFDYDRPLTPEDLEKVEALMQELARNAFSIERQELSKADAIRFFRERGEPYKV
                     EIIEGIPDPTVSLYKQGEFIDLCEGPHLASTAGVAHFKLTTLAGAYWRGDEKNRMLQR
                     IYGTSFPTREELDAHLARLEEAKRRDHRKLGRDLDLFSLTDEVGSGLVLWHPKGARIR
                     RTIEDYWRNEHDRAGYELVYSPHVAKLDLWRRSGHVDFYRENMFTPMDVEGVEYELKP
                     MNCPFHIMVYKSRPRSYRDLPLRFAELGTVYRFERSGVLHGLMRVRGFTQDDAHLFCR
                     PDQLQHEITNVLDFVTTILTTFGFPEYDVYLSTRPEKYVGALERWDLATSALEGALQA
                     KGLPYQIDPGEGVFYGPKIDIKIKDVLRRSWQCATIQVDFNFPDRFGLHYKGEDDKDH
                     QPIMIHRALLGSLERFLGVLIEHYGGAFPTWLAPTQAIVLPIADRVHPYSRDVLAALA
                     ARGIRAELDARNEKIGLKIREAQLAKIPYMLVIGDREAAAGTVSVRRRDGQELPAQAV
                     SAVAEHIETEIRSRVAGPATAQTPKGRA"
     gene            complement(4515..4589)
                     /locus_tag="AC16BC_08480"
     tRNA            complement(4515..4589)
                     /locus_tag="AC16BC_08480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4555..4557),aa:Val,seq:gac)
     gene            complement(4732..5031)
                     /locus_tag="AC16BC_08485"
     CDS             complement(4732..5031)
                     /locus_tag="AC16BC_08485"
                     /inference="COORDINATES: protein motif:HMM:NF037081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3241697.1"
                     /translation="MIHSDQEGRMTKAELIDRMAGTAGISKTSATKALSALVAGITGA
                     LKKGQRVSLVGFGTFAVSKRKARIGRNPRTGEAIKIAATKVPRFSAGKTFKSAIR"
     gene            5220..5600
                     /gene="rnpB"
                     /locus_tag="AC16BC_08490"
     ncRNA           5220..5600
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16BC_08490"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5790..7226
                     /locus_tag="AC16BC_08495"
     CDS             5790..7226
                     /locus_tag="AC16BC_08495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3241698.1"
                     /translation="MRDQKSGGGSRRPAQYQGAALLADPIHGYITFTVPKDGDDAREA
                     TEKDLIDTPWVQRLRYVYQLQSARWVFPSAEHTRFVHSIGAMHVAGRFATHLYPSLKS
                     AVPDCPSAAYVEELMRVTALLHDVGHGPFCHFFDDNFLDQFGVTHEMIGRKIVVDELG
                     AVIRRIRRSPHGPFAPREVLDPEYIGFLICKHSDHDRSKYPTWLQFLQPMIAGIYTAD
                     NLDYVLRDAYMCGVAIGPVDLDRLIHYTFFTDQGWTLHRAGCSALTMFLNARLYLYSN
                     VYYHRTTRAIDLHLRDLFTDTMRIIFPHNPLDRMDAYLGLTDWSLMEEVRGWPRAAGK
                     TKRELGEGWRKILQREVMWKMAYDATLSLRDPGRGQTFVTQEALEAGIRAALPTKLRG
                     LDFRVDLAFQDPRPVNPLRMGGKQIYVYNPSSRTVSKEPLQEFLDFIPAQLVQCRIFA
                     KDHAHDLELAAIAEKVLGQEGAHIKTNV"
     gene            7380..8150
                     /locus_tag="AC16BC_08500"
     CDS             7380..8150
                     /locus_tag="AC16BC_08500"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3241699.1"
                     /translation="MASSVHLSPARRQLNAVSLARALSKLGVTSRSQARGWIEAGRVL
                     VDGRVVRDPDRRIDLDAVKLVVDGKPVAASSRVYLALHKPKGVVTTRSDEQGRETVYG
                     VLARAETADTRWVAPVGRLDRDSSGLLLLTNDTQWGAGISDPGSAVTKTYHVRVQPRI
                     TPEHLSRLSAPVSLVEDFGPPVAVSVVRESPRGTWLEVTLTEGKNREIRRMCARLGYR
                     VETLVRIRIGSIALGDLKPGEIRRLTSKEVAALRVRSR"
     gene            complement(8305..9099)
                     /gene="hemF"
                     /locus_tag="AC16BC_08505"
     CDS             complement(8305..9099)
                     /gene="hemF"
                     /locus_tag="AC16BC_08505"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HIR3241700.1"
                     /translation="MRQQMERYTQDLRDRICRAFEALDTAKFEQRAWQREGGGGGGVM
                     SVMRGTVFEKVGVNCSSVWGDMDPEMAAKLQSKTTEFFATGVSLVAHMANPLVPAVHM
                     NVRYLEMDRWWFGGGTDLTPYYPFEEDTRDFHAALQQACDRHDPSYYPRFKKWCDEYF
                     FLPHRNEPRGVGGIFFDYLNDRPAETLFAFVREVGDTFARIYPEIVARRKALPFTPEQ
                     RKHQLQRRGRYVEFNLIYDRGTLFGLKTKGDVEAILMSMPPVVTWP"
     gene            9452..10273
                     /locus_tag="AC16BC_08510"
     CDS             9452..10273
                     /locus_tag="AC16BC_08510"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241701.1"
                     /translation="MPQPIKAPAKSPVLDPNELLRTERVQDWFLANLRPIMTGVGILV
                     LAGAAWGIVALFQQRAEDRAAALYATANATYQDALAPERQVRAISPETKEMFERAIKE
                     FHAVRGQYPRTAHAALALFYEGNALAGINRFDDAIAAYQTWLSTYTRKDLAPLVAQRL
                     AYALWAKGATQEALARFEDVTKMTDAPNRDLAYFEMGRIQESLGQKDKALETYTALAK
                     EFGASPWSSEGNARIVALGGTPPGAEVPNTSETAPHTPEGAAQPAPTGQQPAPSK"
     gene            complement(10396..11640)
                     /locus_tag="AC16BC_08515"
     CDS             complement(10396..11640)
                     /locus_tag="AC16BC_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3241702.1"
                     /translation="MAIIDEPAPETDEEDAPTYDVPIILNASVEAHIDYFNTRIRDKF
                     ELWLSRSGRYLPLMREIFRQNNLPEDLVFVSLIESGFNPYAYSRARAVGPWQFMKGTG
                     RKYGLRIDEWIDERRDPVKSSYAAAAYLKDLYAMFNSCPLALASYNAGEGRVGRAMKR
                     SKADDFWDLRTTRYLRPETRNYVPKFMAATIIAKNPEKYGFNLNYHEPLRFEEVMVDR
                     PTDLKLIAKTAGVSYEEVKELNPELKQSVTPIDYENYRVKLPFGAKAAFDENFANIPE
                     WEKTVWIKHTVRRGETLASIARKFGTTVPTLRDINHLKRSSVRVGTKILVPTGNRTEV
                     ADDETSSPSSISNASLVVEEPIAEATKPLRYRVKRGDTLWSIAERFNTTVRKIREWNG
                     LGSGSAIRAGQRLKLYVNAEQS"
     gene            12196..13089
                     /locus_tag="AC16BC_08520"
     CDS             12196..13089
                     /locus_tag="AC16BC_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HIR3241703.1"
                     /translation="MPAGISMGGSMVAAARLPAAGAVLVWLAVGAWSSAAWAGSVEYL
                     PGTSPFRPLLADPRETHHSVRYLVGPGRARGEASFGDTFGLVRLSNSPIAQVGIQASV
                     FTRFNSDADSAGFLDINSADYMIFIPVDVRLGAVTVRSGIGHVSSHLGEMEAQHQILD
                     QGASFFDPRFLYRRDYVRVVAAWDVTERLRIYGGASVAVHMKPNRARTAAQAGVEWRG
                     PSRPLGSLLRQWFVGVDLQTWAESDWAANGNWEGGLRLSRPDGERAIRVVVSGYAGRS
                     LQRILAFQREQYLSAGLVFEF"
BASE COUNT         2304 a         4153 c         4366 g         2558 t
ORIGIN      
        1 cagcgcatac gggccgaaga ggtgacggcc gcgcccgttg aacccgaacg atttgtcgcc
       61 gaaataggaa ttcgagatga gaaaggcgtg ctgattcgtg gcgaagaacg gatagtacag
      121 gccgaacgtc agcccggtca ggagcgaccc cttgaggaag agtttgatga acgcccccgt
      181 gtttccgcga aacgcgaacc ggatgttccg ccacgacgta cggctgagcc ggtaccgccg
      241 ggcgccgacg attgcgaccg gcatgaggag cgacaacccc agatacgcgg ccaagaccgc
      301 cgaaatctgg ggcgcgcctt ttggcatcac ttgagaggcg ttcaacaggg cgctgagtcc
      361 gccgaatacc aggcccgcct tcaccgcgcc gcggtagagt tccttgcccg tgccgtgata
      421 ggcgaagcgg tcgccttcga actcggtctg gctcatcagg tacgagcgga cgcggacctt
      481 gccccagaac gagtaaatgc ccagcgtgag cagagtcaga aacacgttca cgatctggat
      541 gccgaacagt gccccgccct gtccctcgaa tgtgaggcgc cgctccgtgg acgcgtcgcc
      601 aaccacctcc gacacggcgg tcacggcagc ggacgcacgc gtcggtttgt ccaccagttc
      661 cgttcgatcc ccctccgcgt cgaacggtgc cggggacgac gccgcggccg gatgcggcgt
      721 cgctggtggc gcgggaggcg ccgtgggccg cggggcttcg gctacgggcc ggcgtgcggc
      781 gggcgctgat gcgctggcct ggcgtataac caggcgtggc gacggagtcc cgcacgactg
      841 gcacgtgggc gcggccagtt gaatgagacg gcacttcgag cagcgcatcg aatcgagcat
      901 ggcctctcct ccactgaggg cgcgtaatac cggaccagtg tagcagagcg tggcaagcct
      961 gtcccttcgc cccgcgcgtt ttcgcggtct ggcaccgaaa agtggatagc accgaagaag
     1021 ggataggcta ctttcctgag acgaggaaat tgattgttac gaattgaagg gaacgttttg
     1081 gacagggggt cagaaagcga aaggggcgct tgcgcgcccc tttcgctttc tgggaattct
     1141 tctgaactcg acttacgccg cagccgcggt tttggtcttg gcttggctca cgactttcga
     1201 gaacccggtc gggtccgcga tcgccaggtc ggacaacact ttgcggttga gtgtgatgcc
     1261 caagttcttc agcgcggcca tgaactggct gtaggacagg ccttccgcgc ggacggccgc
     1321 ggagatgcgg gcgatccaga ggcggcgaaa gtcgcgcttg cgggcgcggc ggtcgcggaa
     1381 cgcgtactgc aggcccttgt ccaccagttc ggtcgcggtg cggaagagtc ggctcttccc
     1441 gccgtacgcg cccttggcga gcttgaggcg cttgcggcgc cggtccttgg tttttggtcc
     1501 acctttaaca cgaggcatgt tgtactcctt tcttttcctt cgtcagtagc ccttcggcct
     1561 aggttttccc agtgccgtaa gggagtaagc gcttgatcat ggattgttcc gagggttcca
     1621 gcaccacgga cgtgctgtag ccgcgtttgc gctgcgcgtt cttgtgcgtc agcaggtggc
     1681 ggttgcccgc gcgcttacgc gagatcttgc cgctccccgt ggtccggaag cgcttgaacg
     1741 cgcccttgtg cgttttcatc ttctttcccg ttcgtggcat tcgatgtcct ttccagtgcg
     1801 accgacttcg cgacgccgat cgacattatg actatccgcc gccgtgtttc ggcgcgagga
     1861 ggatgctcat gttgcggccc tccatgcgcg gctgctgttc aacttgcgcc gcggcgccca
     1921 gttgctcgat gaccttcgcc aaaatggtgc gccccgcgtc ctgatgggcc atctcccgcc
     1981 cgcggaacat caccgtgatt tttaccttat gtccctcggc caggaagtcc cgcgcgtggc
     2041 gggtcttaaa gtccaagtcg tgcgtgtcgg tgaaggggcg aagcttcacc tctttgagct
     2101 gcgccgactt ctgcttggcc ccttgctgct tctttgactg ctcgtagcgg tacttcccgt
     2161 agtcgatgat ccgacacacg ggcggaacgg ccgtcggcga cacctccacg aggtcaagcc
     2221 ccgcttcctc cgcgcgtttg agcgcttcgc cgatcggcag cacgccgatc tgttcggagt
     2281 tgggcccgat gacccgcacc tcgcggattc tgatttcctg attgacgcgg tgttttactg
     2341 cgatagacgg cctccttttg tacggtcagc gcgcggctgg ggctgacgat cacggaacac
     2401 gcgcccggcg gctgagagga ctgcgaatcc tcgggtgagg gagtcgggcg tcatcacgcc
     2461 cgacctttgg gagtctgcgc ggtggccggc ccggccaccc gcgaacgaat ttcggtctcg
     2521 atgtgttccg cgacggccga gaccgcctgc gcgggcagtt cctggccgtc gcgccgtcgc
     2581 accgacacgg tgccggccgc ggcttcgcga tcgccgatca cgagcatgta ggggatcttg
     2641 gccaactggg cttcgcggat cttcagcccg attttttcgt tgcgcgcgtc gagttcggcc
     2701 cgaatgccgc gcgccgcgag cgccgcgagc acgtcgcggc tatacggatg cacgcgatcc
     2761 gcgatcggca acacgatggc ttgcgtgggc gcgagccacg tgggaaaggc gccgccgtag
     2821 tgttcgatca acacgcccag gaaccgctcc agcgatccca agagcgcgcg gtggatcatg
     2881 atgggctgat gatccttgtc atcttcgccc ttgtagtgca ggccgaaacg atccggaaaa
     2941 ttgaagtcca cctggatcgt ggcgcactgc cacgagcgcc gcagcacgtc tttgatcttg
     3001 atgtcgatct tggggccgta gaacacgccc tcgcccggat cgatctggta cggcaacccc
     3061 ttggcttgca gcgcgccttc cagcgccgag gtggcgaggt cccaccgctc caaggcgccc
     3121 acgtattttt cgggccgcgt ggagaggtac acgtcgtact ccgggaaccc gaaggtcgtc
     3181 agaatggtgg tcacgaagtc gagcacgttg gtgatttcgt gctgcaactg gtcgggccgg
     3241 cagaacaggt gcgcgtcgtc ctgggtgaac ccgcgcaccc gcatcaagcc gtgcagcacg
     3301 ccggagcgct cgaagcgata caccgtgccg agttcggcga agcgcagcgg caggtcgcgg
     3361 tagctgcgcg gccgcgactt gtagaccatg atgtggaagg ggcagttcat gggcttgagt
     3421 tcgtactcca cgccctccac gtccatggga gtgaacatgt tctcacggta gaaatccacg
     3481 tgcccgctgc gccgccacaa gtcgagtttg gccacgtgcg ggctgtagac cagttcgtag
     3541 cccgcgcggt cgtgttcgtt gcgccagtag tcctcgatgg tccgccggat gcgggcgccc
     3601 ttggggtgcc acaacaccag gcccgagccc acttcgtcgg tcagggagaa gagatccaga
     3661 tcgcggccga gctttcggtg gtcgcggcgt ttggcttctt ccagtcgggc cagatgcgcg
     3721 tccagttcct cgcgcgtggg gaacgaggtg ccgtagatgc gctgcagcat ccggttcttc
     3781 tcgtccccgc gccaatacgc gccggccagg gtggtgagtt tgaagtgcgc gaccccggcc
     3841 gtgctggcga ggtgcgggcc ctcgcacagg tcgatgaact cgccctgttt gtacaagctg
     3901 accgtgggat cggggatgcc ttcgatgatt tcgactttat acggctcgcc gcgctcgcgg
     3961 aagaaccgga tggcgtcggc cttggaaagt tcctggcgct cgatgctgaa cgcgttgcgc
     4021 gccagttcct gcatcagggc ctcgaccttt tccaggtcct cgggcgtgag cggccggtcg
     4081 tagtcgaagt cgtaatagaa cccgtcttcg atcgcgggac cgatggcgag tttgatgccg
     4141 gggaacaggc gtttgaccgc ctgcgccatg aggtgcgcgg agctgtgacg gaacacctcg
     4201 cggccctcgg cgctgtcgaa cgagagcggc tccacccgcg cgtcgtgcct cagcggcgcg
     4261 tggaggtccg ccacgtgccc gttcaccttg agggcgatgg tgcgggcccg gcgcgcggag
     4321 tccaagctgt cgatcacggc ctgcggggtg accccggccg ggaacgatcg gctggtctga
     4381 tccggaaacg tcaacacaat ctctgaaggc atcaattccc cgaacgcact gcgttgtctc
     4441 tcaaaatggt cggctgagtc gcgcacatac tctcaggcgg atacgccgcg cgcaagtccg
     4501 ccttgagccg gtggtggtag gcacgggcgg tcttgaaccg ccgacctcta ccgtgtcaag
     4561 gtagcgctct ccccctgagc tacgtgccta tcccaccgcg aaagtgctca tcgtacttaa
     4621 tgacttttga tgttgtcaag cggtcgtcat gacgcgaaac ggcggtagcg gaagcccgcc
     4681 accgccgttt cataccagcg tgtccgcgac gtgcgttgcg ggggcgattc gtcagcgaat
     4741 ggcggattta aacgtcttgc cggccgagaa acgcggaacc ttcgtggcgg cgatcttgat
     4801 tgcttcgccg gtgcgcgggt tgcggccgat gcgcgccttg cgcttggaca ccgcgaaggt
     4861 gccgaatccc accagcgaga cgcgctgacc cttcttgagc gcgcccgtga tgccagcgac
     4921 cagggcgctc agcgccttcg tggccgacgt tttggagatg cctgccgtac ctgccatgcg
     4981 gtcaatcagt tctgccttgg tcatgcgtcc ctcctggtcc gagtgaatga tgagcgttcc
     5041 cctactgccc gatcccacgg ccctcgtggc tcccgcggcg cgtccagacg cgacgaaccg
     5101 cggctccgca tacctctaaa tggcccgcag aatagcaagg tcccgcgagc cctgtcaagc
     5161 gcggagcggc gttccgttta cattgtctga tgccgctgcg tataatccgt gcgcacgcca
     5221 gagcggaacg gatgatcgct ccggttgttc cggagaggaa agtccgaact ccgaagggca
     5281 gggtgctccg taacgcggag gcctcgcaag gggacggaaa gtgccacaga aaacaaaccg
     5341 ccccgccggg atttcccggt agggtaaggg tgaaacggtg gggtaagagc ccaccgcccc
     5401 gagggtgacc aagggggcac ggcaaacccc acccggagca agaccaaata ggaggacgct
     5461 ctccctcggg agaacagggt ggcccgccct agtcctcggg tcaggtcgca ggaggccgcg
     5521 ggcaaccgcg gtcccagaga aatgatcatc gcccgccgga gacggcgggg acagaattcg
     5581 gcttatgttt cgctctggcg tgccgcactc ataagccgac cgtcgatacg gggcgactgc
     5641 ggcgttgtcg ggtcggtttc gctcctcacg tacgacacag tacgctccgg ggcgaaacct
     5701 tccctccgcc ttgcattcgc cctcgtctcg gcggtcggcc cggcgtaaca gaacgccgga
     5761 tggtcaaaac cgaggacata tagaccaaca tgcgagatca gaaatcgggc ggtgggtctc
     5821 ggcggccggc gcagtatcag ggggcggcgc tgctggcgga tccgattcac gggtacatca
     5881 cgttcacggt gcccaaggac ggggatgacg cgcgcgaggc cacggagaag gatctgatcg
     5941 acacgccgtg ggtgcagcgg ctgcggtacg tgtaccagct gcagagcgcg cggtgggtgt
     6001 ttccgtcggc cgaacacacg cggttcgtcc actccatcgg cgccatgcac gtggcggggc
     6061 ggttcgccac gcacctgtac ccgtcgctca agtccgcggt gcccgactgt ccctccgccg
     6121 cgtacgtgga agagttgatg cgcgtgaccg cgctgctaca tgacgtgggg cacgggccgt
     6181 tctgtcactt cttcgacgac aactttctcg accagttcgg cgtcacgcac gaaatgatcg
     6241 gccgaaaaat cgtggtcgac gaattgggcg cggtgatccg ccgcatccgc cgcagcccgc
     6301 acggaccgtt cgccccgcgc gaggtgttgg atcccgagta catcgggttt ctcatctgca
     6361 aacacagcga ccacgaccga tcgaagtacc cgacgtggct tcagtttctc cagcccatga
     6421 tcgcggggat ctacaccgcg gacaatctcg attacgtgct gcgcgacgca tacatgtgcg
     6481 gggtggcgat cggtcccgtg gatttggatc gactgatcca ctacacgttt ttcaccgacc
     6541 agggctggac cctgcaccgc gccggctgct ccgcgctgac catgttcctc aacgctcgcc
     6601 tgtacctgta cagcaacgtg tattaccacc gcaccacccg cgcgatcgac ttgcacctgc
     6661 gggacctgtt tacggacacg atgcggatca tcttccccca caacccgctg gatcggatgg
     6721 acgcgtatct cggattgacc gactggtcgc tgatggagga ggtgcgcggt tggccgcggg
     6781 ccgcgggcaa gaccaagcgc gagttgggag aaggctggcg caagattctg cagcgcgaag
     6841 tgatgtggaa gatggcgtac gacgccacgc tgtcgctgcg cgaccccgga cgggggcaga
     6901 cgttcgtcac gcaagaggcg ctggaagcgg ggattcgggc cgcgctgccg accaaactcc
     6961 gagggctcga ttttcgcgtg gacctcgcgt ttcaagaccc gcggccggtc aaccccttgc
     7021 gcatgggcgg caaacagatc tacgtgtaca atccgtcttc gcgaacggtt tccaaggagc
     7081 cgctccagga gttcctggac ttcatccccg cccaactggt gcagtgccgg atcttcgcga
     7141 aagaccatgc ccatgatctg gaactggcgg cgatcgcgga aaaagtgttg ggccaggagg
     7201 gagcgcacat caagacgaac gtgtgatgcg ctcgcagcag acagcccacc gaagaacaag
     7261 tcgttgtcaa tgttgttcct gtaagtgtcc atgagccggt ggctcacccg acgaagatga
     7321 aaaggcaccc cccaccctcc cctccccctc caggggggag ggcaagggag ggggtggccg
     7381 tggcttcttc tgtgcatctg agccctgcga gacgtcagct caacgccgtc agtctggccc
     7441 gtgcactctc caagttgggg gtcacttcgc gcagccaggc gcgtgggtgg atcgaagcgg
     7501 ggcgcgtgtt ggtggatggg cgggtggtgc gcgaccctga ccgacgaatc gatctggatg
     7561 cggtgaaact tgttgtcgac gggaaaccgg ttgcggcgag ttctcgcgtc taccttgcgc
     7621 tacacaagcc caagggcgtg gtcaccaccc gatccgatga acagggacgc gaaaccgttt
     7681 acggggtcct cgcccgtgcc gagaccgcgg atacacgatg ggtcgcgccg gtcgggcggt
     7741 tggatcgcga cagctcgggg ctgttgctgc tgaccaacga cacccaatgg ggcgcgggga
     7801 tcagtgatcc gggatcagcc gtgaccaaga cgtatcacgt ccgcgtacag ccccggatca
     7861 cgcccgagca cctctcgcgt ctgtccgcgc ccgtctccct ggtcgaggac ttcggtcccc
     7921 cggtcgcggt ctccgtggtg cgcgagtccc cgcgcgggac gtggttggag gtgaccttga
     7981 ccgaaggtaa gaaccgcgaa atccgccgca tgtgcgcgcg tctgggctac cgcgtggaga
     8041 ccttggtccg aatccggatc ggctccattg cgctcggcga cctcaaaccc ggcgagatcc
     8101 ggcggttgac ttcgaaggaa gtggccgcgc tgcgggtccg cagccggtaa atggtttgtc
     8161 cttgaaaccg ggcgaagccc gcaggctgct caaaaagctc cggctgcaag gccgcaggga
     8221 ggaggaaacc ggagcgtact gtgtcgtacg tgaggatttc cgacgaccga gaacgccgca
     8281 gacggacttt ttcagtagcc tgccctaagg ccaagtgacg acgggcggca tcgacatgag
     8341 aatcgcctcc acgtccccct tggtcttgag tccgaacaag gtgccgcggt cgtaaatcag
     8401 attgaattcc acgtatcgtc cccgccgttg gagctggtgc ttgcgttgct cgggggtaaa
     8461 gggcaaggcc ttccggcgag ccacgatctc cggatagatc cgggcaaagg tgtcgcccac
     8521 ctcccgcaca aaggcaaaca gggtttcggc cggccggtcg ttcaggtaat cgaagaagat
     8581 gccgcccacg ccgcgcggtt cattccgatg gggcagaaag aagtactcgt cgcaccattt
     8641 tttgaatcgc ggatagtagc tcgggtcgtg ccgatcgcac gcctgctgca aggccgcgtg
     8701 aaagtcccga gtgtcttctt caaaggggta gtagggggtc agatcggtcc cgccaccgaa
     8761 ccaccatcgg tccatctcaa ggtaacgcac gttcatatga accgcgggaa ccaagggatt
     8821 ggccatgtgc gccacgagcg acacgccggt ggcaaagaac tcggtggtct tgctctggag
     8881 cttggccgcc atctcggggt ccatgtcgcc ccatacggat gaacagttca cgcccacttt
     8941 ttcgaacacc gtgccgcgca tgacggacat gaccccgccg ccgcctcctc cttcgcgctg
     9001 ccacgcccgt tgttcgaatt tggcggtgtc caaggcctcg aacgcgcgac aaatccgatc
     9061 gcgcaggtcc tgggtgtagc gttccatctg ctgtcgcatg gccatcgtga ttcgaatccc
     9121 ttgcgttcag gcgcgccatt ggtgcaacca caccgtggcg cgggcggata ccgaaacgtg
     9181 tcgtgaacgg gcgcattata gggtgatcga cgcctgggag gcaagctaac cggagggagt
     9241 aatccggatt gttccggtat cggcaaaccg atctgttcac gagcgggtgc ctcggaaggg
     9301 gggggcgttc ggagtcgctg aaggcatggg atgagcgggg ccgctcgtgc gaggctcgct
     9361 tgagtccctc cccgggctat ggcataatcc gcgggttccg ccgcgtgggt attcgacggc
     9421 ggtggtcgac caacgagaga ggataggtcc cgtgccgcaa ccaattaaag cccccgcgaa
     9481 atccccggtg ctcgatccca acgagctcct tcgcaccgaa cgcgttcagg attggttcct
     9541 tgcgaacctt cgaccgatca tgaccggggt cggaatcctg gtgctcgcgg gcgccgcgtg
     9601 gggtattgtc gcgctctttc agcaacgggc cgaggaccgc gccgcggccc tctacgcgac
     9661 cgcgaacgcc acgtatcagg acgccctcgc gccggaacgg caggttcgcg cgatttctcc
     9721 cgagacgaaa gagatgtttg aacgcgcgat caaggaattt cacgccgtgc gcggtcagta
     9781 tccgcggacc gcgcacgcgg cgctcgcgct gttttacgag ggcaacgcgt tggccggtat
     9841 caaccgattc gacgacgcga tcgcggcgta tcaaacgtgg ctgtcgacct atacgcgaaa
     9901 agacctcgcg ccgttggtcg ctcagcggtt ggcgtatgcc ctgtgggcaa aaggcgccac
     9961 gcaagaggct ttggcgcgct tcgaggacgt caccaagatg acggacgcgc cgaatcgcga
    10021 tctggcgtac tttgagatgg gtcgcatcca ggagagcctc ggccagaagg acaaggctct
    10081 tgaaacctac accgcgctgg ccaaggaatt cggggcgtct ccgtggagca gcgaggggaa
    10141 tgcgcgaatc gtcgcgctgg gtggaacccc tccgggcgcg gaagtcccga atacgtccga
    10201 aaccgctccc cacacgcccg aaggcgctgc gcagccggca ccgaccgggc agcaacccgc
    10261 tccctcgaag tagtccctac cgctcggaag ccgtcgctgg ggcgacggct tccggcgagt
    10321 ctaaggttct cagttcatga atcgggcgga tggagccgtc cgatgaggcg agacggtctg
    10381 agatggtgaa ggctgttagg actgctcggc gttgacgtag agtttcaacc gctgtccggc
    10441 ccgaatcgcg cttccggaac cgaggccgtt ccactcgcgg atcttcctca cggtggtgtt
    10501 gaatcgctcc gcgatgctcc acagcgtgtc gccgcgcttc acgcgataac gcagcggttt
    10561 ggtcgcctcc gcgatcggtt cttccacgac cagcgacgcg ttcgaaatcg aggacgggga
    10621 cgatgtttcg tcgtccgcga cctcggtccg gttgccggtg gggacgagga tcttggtgcc
    10681 gactcgaacg ctggaccgtt tcaggtgatt gatgtcccgc aaggttggaa ccgtggtccc
    10741 gaacttccgg gcgatcgacg ccagcgtctc gccgcgtcga acggtgtgtt tgatccagac
    10801 cgtcttttcc cattccggaa tgttcgcgaa attttcgtca aaggcggcct tggctccgaa
    10861 gggcagcttc acccggtaat tctcgtagtc gatcggcgtc acgctttgtt tcagttccgg
    10921 attgagctct ttgacctctt cgtaggacac gccggccgtc ttcgcgatca acttgaggtc
    10981 ggtcggacga tcgaccatga cctcctcgaa ccggagcggt tcgtggtagt tcagattgaa
    11041 tccgtatttt tccggattct tggcgatgat ggttgcggcc atgaacttgg ggacgtagtt
    11101 gcgggtctcc gggcgcaagt accgggtggt gcgaagatcc cagaagtcgt ccgccttcga
    11161 gcgtttcatg gcgcggccca cgcggccctc tcccgcgttg tacgacgcga gcgcgagcgg
    11221 acacgaattg aacatggcgt acaagtcctt caggtacgcg gccgcggcgt aactcgattt
    11281 gaccggatcg cgccgctcgt cgatccattc gtcgattcga agtccgtact tccggcccgt
    11341 gcccttcatg aactgccagg gcccgaccgc gcgcgcgcgc gaataggcgt acgggttgaa
    11401 cccgctttca atcagcgaca cgaacacgag gtcttcgggg agattgtttt gccggaagat
    11461 ctcccgcatc agggggagat agcgacccga ccggctgagc cataactcga atttgtcccg
    11521 aatcctggta ttgaaataat cgatatgcgc ttccacgctg gcgttcaaaa tgatcggaac
    11581 gtcgtacgtc ggggcgtcct cttcgtctgt ttccggagca ggctcgtcga tgatcgccac
    11641 ctgcgggggc gcctccgcgg gtacgtggac ggtttccgcc ggagcttctg ccggcgtcgg
    11701 ctcggccgga tgttggatga ccgccggctg tgggtgatcg gcgacttcag ccggttgggg
    11761 ctgaaccgcg gggcgctgcg gaaccgttgc cgagggcgac gcggccgcgc gcgtgagggt
    11821 gacggcacgc ggcggctgag gtgttgcgca ggccgcgacg ccaaccaata gaccagtgac
    11881 gacgatgagg cgtttcaacg ggaacctcca aaagttgtgg gcgatgctac tgatttcttc
    11941 gccggactgt caaccagaaa atcccttcct cgcgcgcgaa tgggagaggc gttggtcctc
    12001 gaagtataga cagctatcgg tcgctgtcaa tgccaccgga tcacgaacgg cagtcgccgg
    12061 gccgcttgac acatttcgaa tccccgtgct acatcgggcg cacgcatgac gccatcttcc
    12121 gatctgtgct gattccccat cgagacgcgc gtgctccgct tacgctcttg taatcctgag
    12181 gcgaggagcg cgcgcgtgcc cgcaggcata tccatgggcg gctccatggt cgcggcggcg
    12241 cgcctccccg ccgcgggagc ggtcttggtc tggctcgcgg tgggcgcgtg gagctccgcc
    12301 gcgtgggccg ggtcggtcga atatttgccc gggacctctc cgtttcgccc gctgctggcc
    12361 gatcctcggg agacccacca cagcgtacgg tatttggtcg gaccgggccg cgcccgcggc
    12421 gaggcgtcgt tcggcgacac gttcggcttg gtccgactct cgaattcgcc gattgcgcag
    12481 gtcggcattc aggcatcggt ctttactcgc ttcaacagcg acgcggattc ggccggattt
    12541 ctcgacatca acagcgccga ctacatgatc tttatcccgg tcgacgtccg gctcggcgcg
    12601 gtgacggttc gttccggcat cggccacgtc agttcgcacc ttggcgagat ggaggcgcag
    12661 catcagattc tcgatcaagg cgcgagtttt ttcgatccac gatttctcta tcgccgcgac
    12721 tacgtgcggg tggtcgcggc gtgggacgtc acggagcggc tcaggatcta cggcggcgcg
    12781 agcgtcgcgg tgcacatgaa accgaatcgc gcgcgaaccg cggctcaggc cggcgtggag
    12841 tggcgtggcc cgtcccgccc gttgggttcc ctcctccgcc aatggttcgt gggcgttgac
    12901 ctgcaaacct gggccgagtc cgactgggcg gccaatggaa attgggaagg gggactgcgg
    12961 ttgtctcggc ccgacgggga gcgggcgatc cgggtggtgg tttcgggata tgccgggcga
    13021 tcgctccagc gtatcttggc gttccagcgc gagcagtacc tgagcgcggg actcgtcttc
    13081 gagttctgat cgcctgtcca tgaatggtca tctgcggcgt tgccgctgcg cagccgcccc
    13141 ggccgcctca ccgcctcggc gacgctcgta gcatggcgct ccttcttcgt cgcgccactc
    13201 gccctcacgt acgggttcag tacactctgg tccggtgctc gcggcgcctt gcgacccacc
    13261 gtcaagaaat gggatgtcgg aagtccccga agggggcatc tgacgcgctc ctgaacaggc
    13321 tcgccctgcg tagcagggcg gttaaggctt agaaccttcc agtgtttctc tcacgaattc
    13381 c
//
LOCUS       DBNGTT010000376         3857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_473523, whole genome shotgun sequence.
ACCESSION   DBNGTT010000376 DBNGTT010000000
VERSION     DBNGTT010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_473523"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="AC16BC_08525"
     CDS             complement(<1..309)
                     /locus_tag="AC16BC_08525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR3241704.1"
                     /translation="MGGPGLNLDPSGINRVLIIKLRHIGDVLLTVPTIRAVRERFPNA
                     HITALVLPGTEAMLTGHPCLNEVLTTPVSKGTSSLRRLADEWKFLRTIRAQRFDLTID
                     L"
     gene            complement(291..839)
                     /locus_tag="AC16BC_08530"
     CDS             complement(291..839)
                     /locus_tag="AC16BC_08530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3241705.1"
                     /translation="MIEKLRRFVSHNRIVTSAAFGVLFLILAHPTPNSLLLGLPVVVL
                     GEAVRTWASGYIRKDNRLATDGLYSLTRNPLYLGNFLLGFGFSIMANRWPVILAFLAV
                     FLLIYDATIEEEERKLLKRFGDAFVDYCREVPRFFPRFTGWRAAPFEWALVRQHREFK
                     TWGAVAGIVAVILMKMWWEDLG"
     gene            complement(836..1597)
                     /locus_tag="AC16BC_08535"
     CDS             complement(836..1597)
                     /locus_tag="AC16BC_08535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3241706.1"
                     /translation="MPKLSVTIVAQDEETDIAECVASAAWADEVVVVDGGSRDRTVEL
                     AERAGARVIRQPWMGYAAQKNFAIERATHDWVFSLDADERVTPDLREEIARVLGTASP
                     RDGYYVPRRSYFLGQWMRHGGWYPDYNLRLFRRDKGRFRPREVHECVDVAGSVGYLTA
                     PIDHYTYRTLSEYAQRMDRYSSLAATELLKRGSRPGWTDVVVRPALTFARMFVAKAGF
                     RDGMRGLLLAGCYASYTFFKYAKAWEAARASGRAS"
     gene            complement(1762..1929)
                     /locus_tag="AC16BC_08540"
     CDS             complement(1762..1929)
                     /locus_tag="AC16BC_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR3241707.1"
                     /translation="MAIDHDLLSILACPKCKGPIRLTEKGDGLVCEACRLRYAIKDGI
                     PVMLIDEAPRI"
     gene            complement(1961..3037)
                     /gene="waaC"
                     /locus_tag="AC16BC_08545"
     CDS             complement(1961..3037)
                     /gene="waaC"
                     /locus_tag="AC16BC_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR3241708.1"
                     /translation="MSSDDARVELGPAPRIAVVKLGALGDVVYAFPLVTALKGRWPGA
                     RVTWIVEARLADLPRLHPGVDDVVTVDTRGWRAALRRGGWRAAWREFRAFQRSVDGRF
                     DAAVDAQGLVKSGVVAWLTRAPIRVGFAAQDCRERLNASFMTHHAARVGPVHAVVKNR
                     GLAAVLGAESATARFEIRPGTDDEAWADAWLERHAPGRPARLITLHPGAGHPGKRWPL
                     DRWVALADRLGDRGWRVVLVAGPDERDAVTATALVATPPTVGALAALLRRAEIVVGGD
                     TGPVHLAAALGRRIVGLYGPSDPATAAPVGAGHRLIKHPCACGWTPGPTFNRRCPERF
                     ACMRAIEVEEAADAVEKQLAGQHA"
     gene            complement(3174..3737)
                     /locus_tag="AC16BC_08550"
     CDS             complement(3174..3737)
                     /locus_tag="AC16BC_08550"
                     /EC_number="3.1.3.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034200 - D-glycero-beta-D-manno-heptose
                     1,7-bisphosphate 7-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-alpha-D-manno-heptose-1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR3241709.1"
                     /translation="MSRPLDIVFLDRDGTLNLDEGYLADPERLVLLPGAAEAVAALNA
                     AGVKAVVITNQSGVGRGLIAPEALVRIHERLAALLARGGAKLDGIYSCLHRPEDGCAC
                     RKPATALALQAARDLGVDASRSAMIGDKPVDLELGRRLGGRSVLVRSGEGEKTAAELD
                     AAPGPDYIARDVYDAVQWLVFGARGRP"
BASE COUNT          652 a         1460 c         1180 g          565 t
ORIGIN      
        1 caggtcgatc gtgaggtcga accgttgcgc ccggatcgtc cgaaggaatt tccactcgtc
       61 cgcgagccga cgcagcgagg atgtgccctt cgacaccggg gtcgtcaaca cctcgttcag
      121 acacggatgg ccggtgagca tggcttcggt gccggggagg accagcgcgg tgatgtgggc
      181 gttcgggaac cgctcgcgca ccgcgcgaat cgtgggcacg gtgagcagga cgtcgccgat
      241 gtggcgcagc ttgatgatca acacccggtt gatcccggac ggatcgaggt tcagcccagg
      301 tcctcccacc acatcttcat gagaatgacg gccacgatcc ccgccaccgc gccccaggtc
      361 ttgaactcgc ggtgctggcg caccagcgcc cactcgaacg gcgcggcgcg ccagcccgtg
      421 aaccgcggga agaagcgcgg aacttcccgg caatagtcga cgaacgcgtc cccgaaccgt
      481 ttgagcagct tgcgctcttc ttcttcgatg gtggcgtcgt agatcaacag gaacacggcc
      541 aggaacgcca ggatcaccgg ccaccgattg gccatgatgg aaaacccgaa tcccaggaga
      601 aagttcccca gatacagcgg attgcgggtc agcgagtaga ggccgtcggt cgcgagccgg
      661 ttgtccttgc ggatataccc cgacgcccag gtgcggaccg cctcgcccaa cacgaccacg
      721 ggcagtccga gcagcaggga gttcggcgtg ggatgggcca gaatcagaaa cagcacgccg
      781 aacgccgcgc tcgtcacgat acggttgtgg ctgacgaagc gacgcagctt ttcgatcacg
      841 atgcgcgtcc cgacgccctc gccgcctccc acgccttcgc gtacttgaaa aacgtgtacg
      901 acgcgtagca ccccgccagc aacaatccgc gcatcccgtc gcgaaacccc gccttggcca
      961 cgaacatccg cgcgaacgtc agcgccggcc gcacgaccac gtcggtccac ccgggacggc
     1021 ttccgcgttt gagcaattcg gtcgcggcca gactcgaata ccggtccatc cgctgcgcgt
     1081 actcggacaa cgtgcggtag gtgtaatggt cgatgggcgc ggtcagatac cccacgctgc
     1141 cggccacgtc cacgcactcg tgcacttcgc gcggccggaa gcgccccttg tcgcgccgaa
     1201 acagccgcag attgtaatcg ggataccacc cgccgtgccg catccactgc cccaggaaat
     1261 aactccgccg cggcacgtag tacccgtcgc gcggcgacgc cgtccccaac acccgcgcga
     1321 tctcctcgcg caagtccggc gtcacgcgct cgtccgcgtc gagactgaac acccagtcgt
     1381 gcgtcgcccg ctcgatggcg aagttctttt gcgcggcgta tcccatccag ggttgacgga
     1441 tcacccgcgc gcccgcgcgc tcggccagtt ccacggtccg gtcgcggctt ccgccgtcca
     1501 ccacgacgac ctcgtcggcc cacgccgcgc tggccacgca ctccgcgatg tccgtttcct
     1561 cgtcctgggc gacgatcgtg actgataatt tcggcatcgt cgcgtcagtt caaattcctt
     1621 cccaccccaa tccccttgag cctgttgagg aggggccgga tgcaaggcgc cgcgagcacc
     1681 ggagcggagc gtactgtatg cgtacgtgag caccggatgc acagcggcaa cgccgcagac
     1741 ggcccctcat cgacaggcct cctagatccg cggcgcctcg tcgatcagca tcaccggaat
     1801 gccgtccttg atcgcgtacc gcaggcggca cgcctcgcac accaacccgt cgcctttctc
     1861 ggtgagacga atcggcccct tgcacttcgg gcacgccaga atactgagca aatcgtggtc
     1921 aatcgccata ccccctccag gttctcgcgc cggccgtgcg ttacgcgtgt tgaccggcga
     1981 gttgtttttc caccgcgtcc gcggcttcct ccacctcgat ggcccgcatg caggcgaatc
     2041 gctcggggca ccgccggttg aacgtgggcc ccggcgtcca cccgcacgcg cagggatgtt
     2101 tgatcagacg atggcccgcc ccgaccggcg ccgcggtcgc ggggtcggac ggcccgtaca
     2161 accccacgat ccgccgtccc aaggccgcgg ccaggtggac ggggcccgtg tcgccgccga
     2221 ccacgatctc ggcccgccgc agcaacgcgg ccagcgcgcc cacggtgggc ggcgtcgcca
     2281 ccaacgcggt cgcggtcacc gcgtcgcgtt cgtcgggtcc cgccaccagc acgacgcgcc
     2341 acccccggtc gccgagccga tccgccagcg ccacccaccg atccagcggc cagcgctttc
     2401 cgggatgccc cgcgccggga tggagcgtga tcagccgagc cggccgaccc ggcgcgtggc
     2461 gctcgagcca cgcatcggcc caggcctcgt catcggtccc gggacgaatc tcgaatcgag
     2521 ccgtcgcgct ctccgcgccg agcacggccg ccaacccgcg gttcttcacc accgcgtgga
     2581 ccggccccac ccgcgccgcg tggtgggtca tgaacgaagc gttcaaccgc tcgcggcaat
     2641 cctgggccgc aaaccccacc cggatcggcg cccgcgtcag ccacgccacc accccgcttt
     2701 tgacgagtcc ctgcgcgtcg accgccgcgt caaagcgtcc gtccaccgat cgctggaacg
     2761 cgcgaaactc ccgccacgcc gcccgccacc cgccccggcg caaggccgcg cgccaccctc
     2821 tggtatccac cgtgaccacg tcgtccacgc ccggatgcag ccgcggcaga tccgccaggc
     2881 gcgcttcgac gatccacgtc acccgcgcgc ccggccaccg gcccttgagc gcggtgacca
     2941 gcggaaacgc atacaccacg tcccccagcg ccccgagctt gaccaccgca atccgcggcg
     3001 ccggcccgag ttccacgcgc gcgtcgtcgc tagacaagca agctctccga tgacaccgtc
     3061 atcggaggcc gttcaggaat gtgtcggctg caaggcgccg cgagcaccgg agcggagcgt
     3121 actgtgtcgt acgtgagcac cggatgcgca gcggcaacgc cgcagacgac cattcatgga
     3181 cggcctctgg ctccaaaaac gagccattgt actgcgtcat agacatcccg cgcaatataa
     3241 tccggccccg gcgcggcatc caactccgcg gcggtcttct cgccctcgcc gcttcggacc
     3301 aggaccgatc gcccgcccaa tcgacggccg agttcaagat ccaccggttt gtcgccgatc
     3361 atcgccgacc gcgacgcgtc caccccgagg tcgcgcgccg cttggagcgc gagcgcggtg
     3421 gccggtttgc ggcacgcaca cccgtcctcg ggccggtgaa gacacgagta gatcccgtcc
     3481 agcttcgcgc cgccgcgcgc gagcaacgcg gccagtcgtt cgtgaatcct caccagcgcc
     3541 tcgggtgcaa tcagtccccg ccccacgccc gactggttgg tgatcacgac cgctttgacg
     3601 cccgcggcgt tgagcgcggc caccgcctcc gcggcgccgg gcaggagcac gagccgctcc
     3661 gggtcggcca gatacccctc gtcgaggttg agggtgccgt cgcggtcgag gaatacgatg
     3721 tcgagcgggc ggctcatggg tcacgagcaa atcccccctt accccccctt gacaacgggg
     3781 ggacgagggg attggttgac gcggcgctcg cgaccgccgt gtagacctcg tccactccca
     3841 ccccggtcat gcaccga
//
LOCUS       DBNGTT010000377         4252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3156882, whole genome shotgun sequence.
ACCESSION   DBNGTT010000377 DBNGTT010000000
VERSION     DBNGTT010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3156882"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="AC16BC_08555"
     CDS             <1..456
                     /locus_tag="AC16BC_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241710.1"
                     /translation="LPQTSNEQAVRALIDRRLLLNEADRFSIPIPETTDIAARTSAIT
                     TRLGGPEALTRALARLGWEQGDLKAWVTDELRVTEFLNQRIYFFIIIPPQDVDAYYES
                     HREEFAGLSVDEARDLAGKRLVRERGDVKRDQFLSGLREKATIRLNPIE"
     gene            complement(566..1360)
                     /locus_tag="AC16BC_08560"
     CDS             complement(566..1360)
                     /locus_tag="AC16BC_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3241711.1"
                     /translation="MEWWVLYVVVGIAAGLLAGLLGVGGGVVVVPCLVFAFHVQGLAD
                     QYVMPLALGTSLAAIVVTAAASVRAHDGRGAVEWMVVHRLAPGLALGALGGAWCATWL
                     SSNVLKGLFAGFLWLVATRLVFDGAPRPTRALPGAGGLFVVGGAIGMVSGMTGIGGGT
                     LSVPFLLRHRVPIHRTIGTAAAAGLPIAVAGATGYLVTGLAVSGLPSQSLGFIHLPAL
                     AGIAIASVATAPMGAALAHRMPTHRLKTVFAGLLYLVGARLAFSAF"
     gene            complement(1530..1886)
                     /locus_tag="AC16BC_08565"
     CDS             complement(1530..1886)
                     /locus_tag="AC16BC_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3241712.1"
                     /translation="MPNPFIHVELQTRDLDNAKTFYAALFDWRLEDVPGLGYTMIEVG
                     EGTGGGMMQAKSPDAPAQWIPFIAVGDVRATTDRVIALGGRILKGPSEVPGYGWYSVI
                     TDPTGATFGIWKPERT"
     gene            complement(2082..2549)
                     /locus_tag="AC16BC_08570"
     CDS             complement(2082..2549)
                     /locus_tag="AC16BC_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3241713.1"
                     /translation="MAVEDRLRGLGLELPPAPKPVASYVPAVRSGNLLFLSGILPMKD
                     GKPGWTGKLGRELTVEQGAEAARLACLNALAVVKAELGSLDAVTRIVRLGGHVASADG
                     FTQQPAVVNGASDLLVAIFGDAGRHARLALGAAELPLGAAIELELIVEIGNRR"
     gene            complement(2549..3553)
                     /locus_tag="AC16BC_08575"
     CDS             complement(2549..3553)
                     /locus_tag="AC16BC_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR3241714.1"
                     /translation="MARSRSPEAFQPPWWLRNRHAQTIWRRVAGSTPRVDYRRERVDT
                     PDGDFIDLDWLDGPTSDRDTPLLVVLHGLEGSSRAKYVLGLLSIASASGWNGVAVNFR
                     SCSGELNRLARFYHSGETEDLNWVISLLIARSPHRPLAIVGYSLGGNVLLKWLGERGD
                     TTPEPVRAAVAVSVPYDLGVAAHRVDHGFGRVYGQVFLRTLKTKALAKAERFPGLVDE
                     GRVRRLSRFAAFDEHVTAPIHGFAGARDYWTRSSCVPWLDRICRPTLMISAADDPFLP
                     SAYWPREAVARSSWLETAFSERGGHVGFVQGPWPWTPLYWVDQRAAAFLETIRSKEGF
                     "
     gene            complement(3559..>4252)
                     /gene="lgt"
                     /locus_tag="AC16BC_08580"
     CDS             complement(3559..>4252)
                     /gene="lgt"
                     /locus_tag="AC16BC_08580"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3241715.1"
                     /translation="ARKGLPISDDDKWSFLTLVLFAGILGGRLYYVAFNWRYYGANPG
                     EIPAIWHGGLAIHGGLIGGTLAGWWWVRKHRIPFWKLADASAPSILLGQAFGRFGNFM
                     NGEIHGYPTTLPWGIVFPPNSPAGHDFPGMAVHPAMLYELVLNVIGFAILWSLRRRPT
                     RDGFQICLYLILYGVIRAFVSSFRVEDLMIGGVRAPYVVSAAMVVVGVGAILAFRLWS
                     GDQEASPRRSRA"
BASE COUNT          823 a         1472 c         1300 g          657 t
ORIGIN      
        1 cttccccaga cttcaaacga acaggcggtg cgcgcgctga ttgatcggcg gttgttgctc
       61 aacgaggctg atcggttctc gattcccatc cccgagacca cggacatcgc cgccagaacc
      121 tcggccatca ccacccgcct cggcggcccc gaggcgctga cgcgggcatt ggcccgtctg
      181 gggtgggaac aaggtgattt gaaggcatgg gtcaccgacg aactccgcgt gaccgagttc
      241 ctgaatcagc ggatttattt tttcattatc attccaccgc aggacgtgga cgcgtactac
      301 gagagccacc gcgaggagtt cgctggcctc tcggtcgacg aagcgcgcga cctcgcggga
      361 aaacggttag ttcgggagcg aggagacgtg aaacgcgacc agtttctctc gggtttgcgt
      421 gaaaaagcga cgattcgcct caatcctatc gagtaaattc accggttggc tgtcacgatg
      481 acgcggtagc cgggaatgtc cgccgtattt agggtccgtt caagaatggg gcagattgag
      541 gtagccccga tttcctggac agaccttaga atgcggagaa ggccagcctc gcgcccacga
      601 gatagagcag ccccgcaaac acggtcttga gccgatgggt cggcatccgg tgagccagcg
      661 cggcgcccat cggcgcggtg gccacactcg cgattgcgat gccggccaac gcgggcaggt
      721 ggataaatcc cagactttgc gagggcaatc cggacaccgc gagtccggtg accaggtaac
      781 ccgtcgcgcc ggccacggcg atcggaaggc ccgccgcggc cgcggtcccg atcgtccgat
      841 gaatcggcac ccgatgtcgc agaaggaatg ggaccgagag cgtcccgccc ccgatccccg
      901 tcattcccga gaccatgccg atcgccccgc ccaccacgaa caagccgccc gcgccgggaa
      961 gtgcccgcgt gggccgcggc gccccatcga acacgaggcg cgtggccacc agccacaaga
     1021 atcccgcgaa gaggcccttc agaacgttcg aggaaagcca cgtcgcgcac catgccccgc
     1081 ccaatgctcc gagcgccaag cccggggcga gcctgtgcac gaccatccac tcgaccgccc
     1141 cccggccgtc atgggcgcgc acactcgccg cggccgtcac cacgatcgcg gccaaagacg
     1201 tgcccagagc caggggcatc acgtattggt cggcaagccc ctgcacgtga aacgcgaaga
     1261 ccagacacgg gaccacaacc acgccgcccc caacccccag cagcccagcc aataagcccg
     1321 cggcgattcc gaccacgaca tagagaaccc accattccac gttggaatct tacgcactgc
     1381 ggccaaacgc cgtcaatcac ggggtgttct gatggaacga catgacgcgc tgtagccgaa
     1441 gagagaacgc ccgccggcac tcggcgggcg ttgggcgatg atcggtcctc cgcgtgctgt
     1501 cgcggcacac ggtggaggcc tcgacggtgt caggtcctct cgggcttcca gatcccgaac
     1561 gtggcgccgg tcggatcagt gatgacgctg taccagccgt atcccggcac ctcggacggc
     1621 cccttgagaa ttctgccacc cagggcaatg acccggtcgg tcgtcgcacg gacgtcgccg
     1681 accgcgatga atggaatcca ttgcgcgggc gcatcgggcg atttggcctg catcatgcca
     1741 ccgcccgtcc cctcgcccac ttcgatcatg gtgtagccca agccgggaac gtcttccagc
     1801 cgccaatcaa acaacgcggc ataaaacgtt ttggcgttgt ccagatcgcg cgtctgaagt
     1861 tcgacgtgaa tgaacggatt gggcatggca cctccgaagt ctatgggctc gcggtcctga
     1921 catccctttc aggcggatcg gccttctcgc catcaaccgg attcgtccga tcgactcggg
     1981 cggcgtgtcg tttgtgaatc atcacgtgat tgccgtccgg gtatgcgaca cacgcgatcg
     2041 aggtcatcac gtgcattgct ccagaatgaa ctcagggctg atcagcggcg gttcccgatc
     2101 tcaacgatca attcgagctc gatcgccgcg cccagcggca actcagccgc gcccagcgcc
     2161 aggcgcgcgt gacggccggc gtcgccgaag atcgcgacca gcaggtccga cgcgccgttg
     2221 accaccgcgg gttgttgcgt gaagccgtcg gccgaggcca catggccgcc cagacggacg
     2281 atccgcgtaa ctgcgtcgag actgccgagt tctgctttca ccaccgccag cgcgttcaga
     2341 cacgcgaggc gcgcggcttc cgcgccttgc tccacggtca actcccggcc gagcttgccg
     2401 gtccaaccgg gcttgccgtc tttcatcggc aggatgccgc tcaagaacaa caaattcccg
     2461 ctccgcaccg cgggcacgta cgacgccacg ggtttcggcg ccggcgggag ttcaaggcct
     2521 aacccgcgca atcggtcttc caccgccatc agaaaccctc cttggatcgg atcgtctcca
     2581 gaaacgccgc ggcccgctga tccacccagt acaacggagt ccacggccac gggccttgca
     2641 cgaatccgac gtggccgccg cgttcgctga acgccgtctc caaccaggac gagcgcgcca
     2701 cggcctcgcg cggccaatag gccgacggta aaaacgggtc gtccgccgcg ctgatcatca
     2761 gcgtgggccg acaaatccga tcgagccacg gtacgcagct cgaccgcgtc caataatccc
     2821 gagcgcccgc aaatccgtgg atcggcgcgg tcacgtgttc gtcgaatgcc gcgaacctcg
     2881 acaagcgtcg cacccgtccc tcgtcgacca acccggggaa tcgctccgcc ttggccaggg
     2941 ctttcgtctt caaggtacgg aggaatacct ggccgtacac gcgcccgaac ccgtgatcca
     3001 cgcggtgcgc ggccacgccc aagtcatacg gcaccgacac cgcgaccgcg gcgcggaccg
     3061 gctccggtgt cgtgtcgccg cgctctccca gccatttgag cagcacgttg ccgccgagcg
     3121 aatacccgac gatggcgagg ggacgatggg gtgagcgggc gatcaacaag gagatcaccc
     3181 aattgaggtc ttcggtttct cccgagtgat aaaagcgcgc aagtcgattg agttccccgc
     3241 tgcacgaacg aaagttgacg gccacgccgt tccatccgct cgccgaggcg atggacagga
     3301 gtcccagcac gtacttggcc cgcgacgagc cctccaggcc gtgcagaacc acgagcaatg
     3361 gggtatcccg atccgatgtc ggaccgtcga gccagtcgag gtcgatgaag tctccgtccg
     3421 gggtatcgac ccgctcccgt cggtagtcga cccgtggcgt gctccccgcc acccgccgcc
     3481 agatcgtctg tgcgtggcgg tttcgcagcc accacggtgg ctggaacgcc tccgggctcc
     3541 ggctgcgcgc cacgcccctt acgcgcgaga ccggcgagga gacgcctcct gatccccgct
     3601 ccacagacga aacgcgagaa tggcgcccac gcccaccacg accatcgcgg cgctcaccac
     3661 gtagggcgcg cgcacgccgc cgatcatcag atcttccacg cgaaagctgc tgacgaacgc
     3721 gcggatcacg ccgtacaaga ttaaataaag gcagatttgg aacccgtccc gcgtgggccg
     3781 tcgccgcagc gaccacagga tcgcgaagcc gatgacgttc aataccaatt cgtacagcat
     3841 ggcgggatgc acggccatgc ccggaaagtc gtggccggcc gggctgttcg gcgggaacac
     3901 gatgccccac ggcagggtgg tggggtagcc gtggatctcg ccgttcatga agttgccgaa
     3961 ccgcccgaac gcctggccca ggagaatcga cggcgcgctc gcgtccgcga gcttccagaa
     4021 ggggatccgg tgcttgcgta cccaccacca cccggccaga gtgccgccga tcaggccgcc
     4081 gtggatcgcg agacctccgt gccagatcgc cgggatttcg ccggggttgg cgccgtagta
     4141 acgccagttg aacgccacgt agtagagtcg cccgcccagg atcccggcaa agagcaccag
     4201 ggtcaggaag gaccacttgt cgtcatccga gatcggcaag cccttgcgcg cg
//
LOCUS       DBNGTT010000378         5319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2135880, whole genome shotgun sequence.
ACCESSION   DBNGTT010000378 DBNGTT010000000
VERSION     DBNGTT010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2135880"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..1074)
                     /locus_tag="AC16BC_08585"
     CDS             complement(319..1074)
                     /locus_tag="AC16BC_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241716.1"
                     /translation="MRVPWRIGVGVIALVMSGCGRVAEPVVPTPCSSGECWDLSSGAE
                     VLVNDEPAWAELGPDEAHAWRFHAIPGHQYLVLTRVFSGSADTYASTWPTIDPFTHPM
                     VDVRSGSGLSFTASGSIAYLAVADRGNAAGSAYTVRVVSYDESLDPLPGTARLTVNGL
                     PAPRALASGELARFVFDAIRGADYTVTVITSRGATETYVSLIPSVDDDFFDVAGESGA
                     MPFRATETGAYYVAVVDRSGSAGADIAIRVTSP"
     gene            1185..2063
                     /locus_tag="AC16BC_08590"
     CDS             1185..2063
                     /locus_tag="AC16BC_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3241717.1"
                     /translation="MTGTSSTHRLGMALMLAAAACIGIQGLFVKLAGREIHPVTLAFL
                     RFAASYLLMRAAIGAGWTEVRPVNRRILWVRGILGGIGSLLLFLAVTHTRLSNAVVLF
                     YTYPIFSALIVGLIHGTALSFAVAGAFAASFVGVYLIVQPSFDTVASGDVFGLLAALT
                     AGGAIVSLKVSRATETSWTVFHYYNIAGMLVTAPFLLAYWTTPTIASIPVLLAVLVFS
                     ILGQLGTTYAYRFTTAVEGGVLSMFGAVVGGALGIGALGEEATPHFLAGALLVLLTGI
                     YLTVRGTEDKPVTDRP"
     gene            complement(2121..2456)
                     /locus_tag="AC16BC_08595"
     CDS             complement(2121..2456)
                     /locus_tag="AC16BC_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241718.1"
                     /translation="MSEVEQKVAVAANDDCSVADSADAPPLVVVYDMKGSEVLFRTIR
                     YYDSSMIEAVNDCKMVIGKSFGDGLKGELASRGIEARLTTVDSSDEAVGQIFGIKRGS
                     SKVEMKIQH"
     gene            2765..3040
                     /locus_tag="AC16BC_08600"
     CDS             2765..3040
                     /locus_tag="AC16BC_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241719.1"
                     /translation="MRSTWPSLALAALMAACPIAVHAADDTEDSTRPPQHLVVDSDSA
                     PKVSVTNAVQLVEIVLVPSGEYRPVHPDSVEPDGEPASVEPDAAPAK"
     gene            3078..3530
                     /locus_tag="AC16BC_08605"
     CDS             3078..3530
                     /locus_tag="AC16BC_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3241720.1"
                     /translation="MVKFGERFAAWRTARRMGADDVARRAAMTATRLETIERGDEEPS
                     GSEIEALAAALGVPPGWLYDDPKHLLALIAADGEPCTESDPVTARMLAAAGTERALFT
                     LLTQLVWSGEDRLVRAAEVSLRSLVKQIPRTTVPWESRPPGHFEPPTD"
     gene            3736..>5319
                     /locus_tag="AC16BC_08610"
     CDS             3736..>5319
                     /locus_tag="AC16BC_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR3241721.1"
                     /translation="MTIRDSADQLRRLLARRILVLDGAMGTVLQTKNLTATDFGGPEY
                     EGCNEHLNLTRPDVISSVFEAYFDAGADIIETNTFGSTPLVLAEYGLAHKALEISRAS
                     AAIARKVADARSTADKPRFVAGSMGPTTKTITVTGGVTFDQLIESFTVQAKGLIEGGV
                     DLLLLETAQDTLNLKAAAIGITAAQRALGSRVPLMVSGTIEPMGTMLAGQGVEALYTS
                     LEHLGLFSIGLNCATGPEFMTDHVRSLAGMAMCFVSVYPNAGLPDEEGKYAETPATLA
                     AKLSRFVDEGWINIIGGCCGTTPAHIAAIAKMVAGKQPRAPKTSLRPAVSGIDYLPFE
                     EDNRPVIVGERTNVIGSRKFKDLIIAGKIEEASEIGRAQVKNGAQIVDVCMANPDRNE
                     LADMDAFLAVLTKKVKAPLMIDSTDHMVIEAALTRCQGKCVINSINLEDGEERFEQVV
                     PLIKRYGAAVVVGCIDEDPKQGMAVTRERKLAVAQRSYDLLVNKYGIPPRDLIFDALV
                     FPVGTGDENYIGSAVETIEG"
BASE COUNT          971 a         1779 c         1619 g          950 t
ORIGIN      
        1 atgtcacact aagatatatt caaatcgtgt gccaattatc attatcaaaa atacgtacca
       61 aggcataagg aagaattatc aggttttaca gctggttgta tgaataattg ctcatagacc
      121 gaattagtca caattctttg tagccacggc ttgtgtaaaa aaatcattca cgcctgatat
      181 agccgctctg caataacgac taattattaa tataaatcaa tagtttaatc tgatcagcat
      241 cctgtcagct ttttatacac cttaatcaga gccgccccgg atttcttacg cctcgcagaa
      301 cggcaggaac ttcaaacatc atgggctcgt cacgcgaatc gcgatatcag ccccggcaga
      361 ccctgagcgg tcgaccacgg ccacgtagta ggccccggtt tcagtcgcgc ggaagggcat
      421 cgcgccggac tcgccagcca catcgaagaa atcatcgtcc acggatggaa tcagcgagac
      481 atacgtctcg gtcgcgcccc ggctggtgat gaccgtgacg gtgtagtccg cgccgcggat
      541 cgcatcgaac acaaaccgcg ccaattcccc ggacgccagg gcgcgcggcg ccgggagtcc
      601 gttgaccgtc agacgcgcgg tgcctgggag cggatcgagg gactcgtcgt acgacaccac
      661 gcggaccgtg tacgcgctgc ccgcggcatt gccgcgatcc gccaccgcga ggtaggcgat
      721 gctcccgctc gcggtaaacg acaacccgga gcccgaccgc acgtccacca tggggtgcgt
      781 gaacggatca atagtcggcc aggtcgacgc ataggtatcg gcgctgcccg agaacacccg
      841 ggtcaacacc aaatactgat gtccgggaat cgcatgaaac cgccacgcgt gcgcctcatc
      901 gggccccaat tcggcccagg ccggctcgtc gttgacaagg acctcggctc cgctcgacag
      961 atcccagcac tcgccggagg agcacggcgt cggcacgacg ggctccgcga cccggccgca
     1021 cccggacatc accagcgcga tcactccgac accgattcgc caaggcacac gcatggccgc
     1081 ctccggatca cacgtctgga cactcggtca gacggcgggc gccggagatt attcacgcgc
     1141 gtcactttga ctttaccgcg cccccgtact acgatcgcgc tcccatgacc ggcacgtcct
     1201 cgacacaccg actcggtatg gcgttgatgc tcgcggccgc ggcctgcatc gggatccagg
     1261 gattgttcgt caaactggcc ggccgcgaga tccacccggt cacgctcgcg ttcctgcgtt
     1321 ttgccgcgag ttatctcctc atgcgcgcgg ccatcggcgc ggggtggacc gaggtccggc
     1381 ccgtgaaccg tcgtatcctg tgggtccgcg gcatcctggg cggaatcggc agcctgctgc
     1441 tctttctcgc ggtcacgcac acgcgcctgt ccaacgccgt ggtgctgttc tacacctacc
     1501 cgatcttctc ggcgttgatc gtcggcctga tccacgggac ggccttgtcg ttcgcggtag
     1561 ccggcgcgtt tgccgcgtca ttcgtgggcg tatatctgat cgtgcagccg tcgttcgaca
     1621 cggtggcctc gggcgacgtg ttcgggttac tggcggcgct gacagcgggc ggcgcgatcg
     1681 tctcgctgaa ggtctcgcgc gcgaccgaaa cctcgtggac cgtgtttcac tactacaata
     1741 tcgcgggcat gctggtgacc gcgcccttcc tgctcgcgta ttggacgacg ccgaccatcg
     1801 cgagcattcc ggtgctgctg gcggtcttgg tgttctcaat cttgggacaa ctcgggacga
     1861 cctacgccta ccggttcacg accgcggtcg agggcggggt gttatccatg ttcggcgcgg
     1921 tggtgggcgg cgcgctcggc atcggcgcgc tcggcgagga agccacgccg cactttctcg
     1981 cgggcgcgct cctggtgctc ttgacgggaa tctatttgac ggtccggggg acggaagaca
     2041 agccggtcac cgatcgcccg tgacggcgcg cagccccgag atccgacgtt ccgtgcgccg
     2101 agggcgaggc ccccggctgg ttagtgctga atcttcatct ccaccttgga cgaaccccgc
     2161 ttgatgccga aaatctgacc cacggcttcg tccgacgagt ccacggtggt caggcgcgct
     2221 tcgatgccgc gagaggccag ttcgcccttg agcccgtcgc cgaacgactt gccgatcacc
     2281 atcttgcagt cattgaccgc ttcgatcatg ctgctgtcgt agtaacgaat cgtgcggaac
     2341 agcacctcgc tgcccttcat gtcgtacacc acgaccaagg gcggcgcatc cgccgaatcc
     2401 gccacgctac agtcatcgtt cgccgcgacc gccaccttct gctcgacttc actcatcgtt
     2461 cacccccgga tgacaccgac cctgtttcat tagatcatag accatcgaca acggcccgtt
     2521 attttatcga gattctcccg gacgatgcaa tcgcgcgcat cgcccgactc cccgcgcgca
     2581 tccatttgcc cccaggttga ggactttttc ctcaatcggg ttcgctccca aagccgcatc
     2641 acaggccgct ctcctcgttt tcgccccgct tttatccgcc ggggccgggc gtacggattg
     2701 cattcttcac cgttcacccg atcgccacgc ggcccaaccc tttctccgca aggagtactt
     2761 ggtaatgcgt tcgacatggc cctccctggc attggccgct ctcatggcgg catgcccgat
     2821 cgccgttcac gcggcggacg acaccgagga ctccacgcgc ccaccgcagc acctggtcgt
     2881 ggattcagac tccgcgccca aggtttcggt gaccaacgcc gtccaactgg tcgagatcgt
     2941 gctggtaccg agcggcgagt accgacctgt ccaccccgat tcggtcgagc ccgacggcga
     3001 acccgcctcg gtcgagcccg acgcggctcc cgcaaagtag ctgttttcgt cgccctgatt
     3061 tcgctacaat cgttcccgtg gtgaaattcg gagaacgttt tgccgcatgg cggacggcgc
     3121 gccggatggg ggctgacgac gtggccaggc gcgccgccat gaccgcgacg cgcctggaaa
     3181 ccattgaacg gggcgacgag gaaccctcgg gttccgagat cgaggcgctc gcggccgcgt
     3241 tgggcgtccc gcccggctgg ctctacgacg accccaagca cctgctcgcc ttgatcgcgg
     3301 ccgacggcga gccgtgcacc gagtctgacc ccgtgaccgc gcggatgctg gctgccgcgg
     3361 ggaccgagcg cgccctcttc accctcctga ctcaattagt gtggtccggc gaggatcgct
     3421 tggtccgcgc cgcggaagtg agcctgcgga gcctggtcaa acagatcccg cggacaaccg
     3481 tgccgtggga gtcgcgcccg cccggacatt ttgagccacc cacggactga cgagggctgg
     3541 gagtgtccgt gtctcgggcc cttgataagc ctgtccgcca acgggccgga tgcaaggcgc
     3601 cgcgagaacc ggagcgcagc gtactgtacg cgtacgtgag caccggaagc gcagcggcaa
     3661 cgcggcagat ggcccttgcc ggacaggctc ccgcgcgtag acgcgcctca tcagcctttg
     3721 ttatgatgcc cgaccatgac catccgcgac tccgctgatc aactccgccg cctgctcgcc
     3781 cgccgcatcc tggtgctcga cggcgccatg ggcacggtgt tgcaaaccaa gaatctgacc
     3841 gccaccgact tcggagggcc ggagtacgag gggtgcaacg agcacctcaa tctcacgcgc
     3901 cccgatgtga tctccagcgt gttcgaagcg tactttgacg cgggcgcgga catcatcgag
     3961 accaacacct tcggcagcac gccgctcgtg ctggcggagt acggcctggc tcataaagcg
     4021 ttggagatca gccgcgccag cgccgcgatc gcgcgcaagg tggcggacgc gcgatcaacg
     4081 gccgacaaac cgcgcttcgt ggcggggtcc atggggccca ccaccaagac catcacggtc
     4141 accggcggcg tcacgttcga ccaactgatc gagtcgttca cggtccaggc caagggactg
     4201 atcgaaggcg gcgtggatct gctgctgctg gaaaccgcgc aggacacgct caacctcaag
     4261 gccgcggcca tcggcatcac ggccgcgcag cgggcattgg gctcgcgggt gcccctcatg
     4321 gtctccggca ctatcgaacc catgggcacc atgctcgcgg gccaaggggt cgaagccctc
     4381 tacacctcgc tcgaacatct cggtctgttt tcgatcggcc tgaactgcgc caccggcccg
     4441 gagttcatga ccgaccacgt gcgctcgctc gccggcatgg ccatgtgttt cgtatcggtc
     4501 tatcccaacg cgggtctgcc ggacgaagaa ggcaagtacg cggagacgcc ggcgacgctc
     4561 gcggccaagc tgtcgcgctt cgtggatgaa ggctggatca acatcatcgg cgggtgctgc
     4621 ggcaccacgc ccgcgcacat cgccgcgatc gcgaagatgg tcgcggggaa acaaccccgc
     4681 gcgcccaaga cctcgttgcg gcccgcggtg tccggcattg attatctccc cttcgaggag
     4741 gacaaccgcc cggtcatcgt gggcgagcgc accaacgtga tcggcagccg caagttcaag
     4801 gacctgatca tcgcgggcaa gatcgaggag gcgtcggaaa tcggccgcgc gcaggtcaag
     4861 aacggcgcgc agatcgtgga cgtgtgcatg gccaacccgg accgcaacga gttggccgac
     4921 atggacgcgt tcctcgcagt gctgaccaag aaggtcaagg cgccgctgat gatcgactcg
     4981 accgatcaca tggtgatcga ggccgcgctc acgcgttgcc agggcaagtg cgtgatcaac
     5041 tcgatcaacc tggaggacgg cgaggagcgg ttcgagcagg tggtgccgct gatcaaacgt
     5101 tacggcgccg cggtggtggt cgggtgcatc gacgaagacc ccaaacaggg catggcggtc
     5161 acgcgcgagc gcaagctggc cgtggcccag cgatcgtacg acctgctggt caacaaatac
     5221 ggcatccctc cgcgagattt gatcttcgac gccctggtgt tccccgtggg cacgggcgac
     5281 gagaactaca tcggctcggc cgtggagacg atcgaaggc
//
LOCUS       DBNGTT010000379         3578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3285024, whole genome shotgun sequence.
ACCESSION   DBNGTT010000379 DBNGTT010000000
VERSION     DBNGTT010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3285024"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..1255)
                     /locus_tag="AC16BC_08615"
     CDS             complement(398..1255)
                     /locus_tag="AC16BC_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3241722.1"
                     /translation="MLTPRRKLPDPAQHVEQVKAFYANIAGLDLAQYPYFIRIYQLFD
                     ACQREIFELGRHGGPYESVLDVGCGEGYHSFYAARFAKQVTGLDVSPDALALAEQRAR
                     RERVSNLRFVCSDLTKPPLPDASFDCVFAYGDVIGHIPEYDAALAQLSRLCKPGALMV
                     LDYDNKYHPGLLWDAEERRLARETPEIGHTHAWSFRGRELEFNTFGRREMRDLLARHG
                     FVLERVYGFDFFSFLIPHPERYQFSDRIGFAERAVMALNRLDLACRNWWPVNRWGYTQ
                     IGFARKTAG"
     gene            complement(1304..1951)
                     /gene="rnhC"
                     /locus_tag="AC16BC_08620"
     CDS             complement(1304..1951)
                     /gene="rnhC"
                     /locus_tag="AC16BC_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HIR3241723.1"
                     /translation="MTATLSAWIGVDESGKGDYFGPLVIAAVCVDDETASMLRTSGVR
                     DSKTLSDRTIADLAKEIEGLCRTSVVAIGPARYNELYNKFKNLNRLLGWGHARAIENV
                     LEAGCPASHAISDQFGDERFITSALMEKGRTIRLTQRPRAEEDVAVAAASIMARAEFV
                     KRLDRLSDEAGLTLPKGASNLVDAAAKRLVRAKGKDALTAFAKVHFKTTAKVLAE"
     gene            2241..2612
                     /gene="rpsF"
                     /locus_tag="AC16BC_08625"
     CDS             2241..2612
                     /gene="rpsF"
                     /locus_tag="AC16BC_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3241724.1"
                     /translation="MTVYETIFIVKTSLTDEELSGLVGKMRGVIEKHGGEILKSENWG
                     KKKLAYEVRKEKKGAYVFFRFRGAGSVISELERQYRVEDAIIKFLTVKCDPKLLADEM
                     AREAAAARAAAAPAAAPAESA"
     gene            2649..3080
                     /locus_tag="AC16BC_08630"
     CDS             2649..3080
                     /locus_tag="AC16BC_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3241725.1"
                     /translation="MASFNKVILMGNLTRDPEIRYTPSGTPVATLGLAVNRRYKQGEE
                     LKDEVCYIDVVVFGKQAEHCGQYLNKGHGVIVDGRLQQRRWETDDGQKRSKHEVVAQT
                     INFLPKRPDQAGGGAPSAGKAGSSPDVVLQNEEFADQDVPF"
     gene            3139..3450
                     /gene="rpsR"
                     /locus_tag="AC16BC_08635"
     CDS             3139..3450
                     /gene="rpsR"
                     /locus_tag="AC16BC_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3241726.1"
                     /translation="MRTAPVTSPSASSSAPARGEARQGAGAPSERKFFGRRKACRFCT
                     DKVVGINYKDVSMLRNYLTERGKIMPRRLSGNCARHQRELTLAIKRARNIALVAFTEE
                     R"
BASE COUNT          674 a         1079 c         1189 g          636 t
ORIGIN      
        1 gaccggcctc aggtctccag tagccgggct aattcccgca cgccgcggtt gatacagcaa
       61 tagtggggcc agcgggtttt tccggctttc atggcaatcg ctcatcctaa ggcagctccg
      121 gctcgcaggg ttcagtcgtc ctcgtcaaaa aacattcacc aatatcgtca aataattgac
      181 agtctcgtcc agatgaactg atacgcgaga tcggtgatcg acctgtggag tctggaagtc
      241 tgatcgacat tgatgtggtg ctgcccgtgg ttcgcttcca ccgcagctca tgaaggacga
      301 cgtcgttcgg gtgaagccgg tcacagggct gtgagaaacc gacgcctgcg aagtaggcgt
      361 ggcgttacgg tgccggagtt gtctggcgcg ccgggggcta tccggcggtt tttctcgcga
      421 atccgatctg cgtataaccc caccggttga ccggccacca attccggcag gccaagtcca
      481 ggcggttgag cgccatcacc gcgcgctcgg cgaacccgat gcgatcggag aattggtatc
      541 gttcggggtg cggaatcaga aacgaaaaaa agtcgaatcc atagacccgt tccaggacaa
      601 agccgtgacg cgcgagcagg tcgcgcattt cacgccggcc gaaggtgttg aactccagtt
      661 cccggccgcg aaacgaccac gcgtgggtat ggccgatctc cggggtttcc cgggcgagcc
      721 ggcgctcctc cgcgtcccag agcagaccgg ggtgatactt gttgtcgtag tcgagcacca
      781 tgagcgcccc gggtttgcag aggcgcgaga gttgcgcgag cgcggcgtcg tactccggga
      841 tgtggccgat cacgtcgccg taggcgaaca cgcagtcgaa cgaggcgtcg gggagcggcg
      901 gcttggtcag gtccgaacac acgaaccgca ggttgctcac gcgttcgcgt cgggcccgtt
      961 gctcggccag cgccaacgcg tcgggcgaaa cgtccagccc cgtgacctgt ttggcaaacc
     1021 gcgcggcgta gaacgagtgg tacccctccc cgcagcccac gtccaacacg gattcgtagg
     1081 gcccgccatg ccggcccaat tcgaaaattt cgcgctgaca ggcgtcgaag agctggtaga
     1141 tgcggatgaa gtacgggtac tgcgcgagat cgaggcccgc gatgttggcg tagaaggcct
     1201 tgacctgctc cacgtgttgg gcgggatcag ggagtttgcg acggggcgtc aacatgagag
     1261 cgtcgacatc ggggcggcat tatacgcaag cgtcttgccg aagtcactcc gcgaggacct
     1321 tggccgtggt tttgaagtgc actttggcga acgcggtgag cgcgtccttg cccttggcgc
     1381 gcaccaggcg tttggccgcg gcatccacga gattggaggc gcctttcggc aacgtgagtc
     1441 cggcttcgtc cgagagtcgg tccaggcgct tcacgaactc ggcccgcgcc atgatcgagg
     1501 cggcggccac ggccacgtcc tcctcggcgc gcggccgctg ggtgagccgg atggtccggc
     1561 ctttttccat caacgcgctc gtgatgaacc gttcatcgcc gaactgatcg ctgatggcgt
     1621 gcgacgcagg gcaccccgct tccaggacgt tttcgatggc ccgcgcgtgg ccccacccca
     1681 acagccggtt caggtttttg aacttgttgt agagctcgtt gtatcgcgcc gggccgatgg
     1741 ccaccacgct ggtccggcag agcccctcga tttctttggc gagatccgcg atggtgcggt
     1801 ccgagagggt cttgctgtcg cgcacgcccg aggttcgcag catgctcgcg gtttcgtcgt
     1861 ccacgcaaac cgccgcgatg accagcgggc cgaagtagtc gcctttgccc gattcgtcca
     1921 cgccgatcca cgcggagagg gtcgcggtca tggatatccc gaatcgatag ggcgtgaaca
     1981 ggcggatcgc acggcgagcg actgtagcag aagcggtccg attcgatcaa ggccgcgtcg
     2041 cggccgtcgc gggagggctc aggcgcggac ggaccgcaaa cgcgttcagg ggggggcggc
     2101 gcgctcgccg cgattcgtgc cggcctcgta acgatgcgcc cgacgatcgc cttgctttct
     2161 cgtgacgccc tatgctatag atcacggtcc ttgctctcgc cacggtgcga gggctttaga
     2221 gccaaaagga gccctgtcat atgacggtct atgagaccat cttcatcgtc aaaacctcgc
     2281 tgaccgacga ggaattgtcc ggtctggtcg gaaaaatgcg cggcgtcatt gaaaaacacg
     2341 ggggcgagat cctcaagtcg gagaactggg gcaagaagaa gctggcgtac gaggtgcgca
     2401 aggagaaaaa gggcgcctac gtcttcttcc gattccgcgg cgcgggatcg gtgatctccg
     2461 aactggagcg ccagtaccgg gtcgaggacg ccatcatcaa gttcctgacg gtcaagtgcg
     2521 atcccaaact gctggccgac gagatggccc gcgaggcggc cgcggcccgt gcggcggcag
     2581 cccccgcggc ggcgccggcg gagtctgcgt aatcccacga cgggccgccg cgacgcggag
     2641 gagcatgtat ggcgagtttt aacaaagtga tcctgatggg caatctcacc cgcgacccgg
     2701 aaatccggta taccccgagc ggcacgccgg tggcgacctt gggccttgcc gtgaatcggc
     2761 ggtacaagca aggcgaagag ttgaaagacg aagtctgtta catcgacgtc gtggtcttcg
     2821 gcaaacaggc cgagcactgc ggccagtacc tgaacaaagg acacggggtg atcgtcgacg
     2881 gccggctcca gcaacgccgc tgggaaacgg acgacggcca gaagcggagc aaacacgagg
     2941 tggtggctca gacgatcaat ttcctcccga agcgtcccga tcaagccgga gggggggcgc
     3001 cgtcggccgg gaaagccggc tcgtccccgg acgtggtcct gcagaacgag gagtttgcgg
     3061 atcaggacgt gccgttttga ggattgaccg tcgggcgatg cgctcgcccg cgaaccaaga
     3121 ggagtgaaaa gagaatccat gagaaccgcg ccagtcacat cgccctccgc gtcgtcttcc
     3181 gcgccggccc gtggggaagc ccgtcagggg gcaggggctc cgtcggaacg gaagtttttc
     3241 ggacggcgga aagcctgtcg tttctgcacc gataaggtgg tgggcatcaa ttataaggac
     3301 gtgtcgatgc tccggaacta tttgaccgag cgcgggaaga tcatgccgcg tcggttgtcc
     3361 ggcaattgcg cgcgacacca gcgcgagctg accctggcga tcaaacgcgc ccgcaacatc
     3421 gcgttggtcg cgtttaccga agaacgataa cctccacgct tcggccgtcc ggcgcggttc
     3481 ccttcgacgg gccgccgccc gggttggccg gcggagcggc gccgtatgtt ctcccggtta
     3541 ggcttcctcg cgcgcctcaa taaaaaaacg gtctggat
//
LOCUS       DBNGTT010000380         1559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_345856, whole genome shotgun sequence.
ACCESSION   DBNGTT010000380 DBNGTT010000000
VERSION     DBNGTT010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_345856"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /gene="ccsA"
                     /locus_tag="AC16BC_08640"
     CDS             <1..331
                     /gene="ccsA"
                     /locus_tag="AC16BC_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR3241727.1"
                     /translation="LFFRLGAVSELDTMLHRTMVLGFPLLTLGMLSGAVYSRIAHGVF
                     WGWSAKELWSLAAWAIYAVLLYGHRAWGWHGRRAAFFAIAGFVAYLLAYLGLKFASWL
                     VSRGQYV"
     gene            498..1457
                     /locus_tag="AC16BC_08645"
     CDS             498..1457
                     /locus_tag="AC16BC_08645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005449802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3241728.1"
                     /translation="MITRRPVLLTRTLPPRAMALLQSRVDLTCNPHDRALTRDDLFRM
                     IAGQHGVVTMLTDRVDGELLDAAPDLKVVANYAVGYNNIDLEAARARGIVVTNTPGVL
                     TDATADLTWALILGITRRIGEGDRLVRAGRWSGWTPTQLLGMELRDSVLGIVGMGRIG
                     RAVASRAGAFGMRVVYAARHPVPNAAPAWRAASLDELLATADVVSLHVPLTDATRHLI
                     GSKQLAAMKPAAYLINTSRGPVVDEAALVEALRLGRIAGAGLDVYEREPEIQAGLDAC
                     DNTLLLPHLGSATTATRERMGAMVVENVLAVLEGRRPPNPVEA"
BASE COUNT          242 a          514 c          516 g          287 t
ORIGIN      
        1 gttgtttttc cgcctcggcg ccgtgagtga actcgacacc atgctccacc gcaccatggt
       61 gctcggtttc ccgttgctca cgctcgggat gctcagcggc gcggtctact cccgcatcgc
      121 ccatggcgtg ttctggggct ggagcgcgaa ggagctgtgg tcgctggccg cgtgggcgat
      181 ctacgccgtc ctgctgtacg gccaccgcgc gtgggggtgg cacgggcgtc gcgccgcgtt
      241 tttcgccatc gccggattcg tcgcgtacct ccttgcgtat ctggggctga agtttgcgtc
      301 gtggctggtt tcccgcggcc agtacgtcta ggagcctgtc catgactggt catctgctgc
      361 gttgccgctg cgcgtccggt cctcacgtac acattgagta cgctccggtc cgggcgctcg
      421 cggcgccttg catctgacgc agtcctgaac aggctcccat tacggcatct ccgaatacac
      481 ttccagtcaa attgactatg atcacccgcc ggcccgtgtt gttgacgagg acgctcccgc
      541 cgcgcgccat ggcgctgctg caatcccgcg tcgacctcac gtgtaatccc cacgaccgcg
      601 cgctgacgcg cgacgacctg ttccgcatga tcgccggaca acacggcgtc gtcacgatgc
      661 tgaccgatcg cgtcgacggc gaattgttgg atgccgcgcc ggatctgaaa gtggtcgcca
      721 actatgcggt cgggtacaac aatatcgacc ttgaggccgc gcgagcccgc ggcatcgtgg
      781 tgacgaacac gcccggcgtg ctgaccgacg cgaccgcgga tctgacgtgg gccttgatcc
      841 tcgggatcac acggcgtatc ggcgaaggcg atcggctggt tcgagccggg cgctggagcg
      901 gctggacccc gacgcaactc ctgggcatgg agctgcgcga cagcgtgttg ggcatcgtgg
      961 gcatgggccg gatcggccgc gcggtggcgt cacgcgccgg cgcgttcggc atgcgcgtgg
     1021 tctacgccgc gcgacatccc gtgccaaatg cggccccggc ctggagagcc gcctcattgg
     1081 acgagttgct ggccaccgcc gacgtggtgt cgctgcacgt gcccctcacc gacgcgacgc
     1141 ggcatctgat cggatcgaag cagttggccg cgatgaaacc cgcggcgtat ctgatcaaca
     1201 cgtcacgagg cccggtcgtg gacgaggccg cgctggtcga ggcgctgagg ctggggcgaa
     1261 tcgcgggcgc cggactcgat gtctacgaac gcgagccgga gattcaggcc ggcttggacg
     1321 cgtgcgacaa taccctcctc ttgccgcacc tgggatccgc gaccacggcg acacgcgaac
     1381 gtatgggagc catggtggtc gagaacgtgc tcgcggtgct tgaagggcgc cgcccgccca
     1441 acccggttga ggcataaaaa aaaacggggt gcactaacgt gcgccccgtt cgtcccgaag
     1501 gacattatcc ccgcagggtc tgaagctttt tcaaccgatc cggataccgc agcttccgg
//
LOCUS       DBNGTT010000381         6384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2391108, whole genome shotgun sequence.
ACCESSION   DBNGTT010000381 DBNGTT010000000
VERSION     DBNGTT010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2391108"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16BC_08650"
     CDS             complement(<1..225)
                     /locus_tag="AC16BC_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010035979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241729.1"
                     /translation="MADTTEYRRPASADRDDFGVIHHQPEPAPDDRAVLRTEELLLNM
                     GPQHPSTHGVLRVVLELEGERILKATPHLGY"
     gene            complement(256..765)
                     /locus_tag="AC16BC_08655"
     CDS             complement(256..765)
                     /locus_tag="AC16BC_08655"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3241730.1"
                     /translation="MHPLAQFVQDRFPDAVTGVNEAFGELTISLRKDRLVPICRTLRD
                     DPAWRFNFLSDVLSVDFPDDEERFEVVYHLYSIEKRHRVRLKARVSEDDCRIESVTPL
                     WPGANFMEREVYDLMGIEFLNHPDHRRILLTDDFDGHPLRKDYPTEGKGWRNTFEFLP
                     KGEGPTVSQ"
     gene            complement(769..1248)
                     /locus_tag="AC16BC_08660"
     CDS             complement(769..1248)
                     /locus_tag="AC16BC_08660"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3241731.1"
                     /translation="MGVGGGLDSTIVTTKVDSVINWARKSSLWPMTFGLACCAIEMIT
                     SVSARHDIDRYGAGVFRASPRQSDLMIVAGTVCRKMAPIIRRIYDQMPEPRYVISMGS
                     CATSGNIYNSYSVVQGVDQIVPVDIYVPGCPPRPEALFDGILKLQEQIMQRRVLTKA"
     gene            complement(1239..1610)
                     /locus_tag="AC16BC_08665"
     CDS             complement(1239..1610)
                     /locus_tag="AC16BC_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3241732.1"
                     /translation="MPGEYVPQQYLPILIFTAIAAVFGFAQLFVASFIRPHDPYREKL
                     RPYESGVEPFSDARIPFPMRYYVITMLFVIFDIEAVFLYPWAVVYKQIGLYALVEMVL
                     FIAILVVGYVYAWKKGALEWE"
     gene            1972..3768
                     /locus_tag="AC16BC_08670"
     CDS             1972..3768
                     /locus_tag="AC16BC_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HIR3241733.1"
                     /translation="MPHTPRPRVFRLPSLLLALTLVACGGGGGASDSPPNADLAGLTL
                     SIGSLSPAFDPAATDYTATTGFLSPSVNVTPTAASSGATITVNGVATPSGVSSAPITL
                     AEGDNAVFIDVTASDGTTKRYTVTIAHPNAASLVPPDYLKASNSGASDRFGKSVALDG
                     DTLVVGAALEDSAATGVNSLQGPSDNSATDSGAVYVFVRANGAWSQQAYIKPSNTGAG
                     DQFGFSVALSGDTLVVGAPREDSASTGVGGKQTNNQASNSGAVYVFVRTNGVWSQRAY
                     LKASNSEAQDHFGSSVALAGDTLVVGSPGEDSSATGVNGDQGDNASTDSGAAYVFVRN
                     GTTWQQQAYLKASTTDANPQFGDYPDQFGFSVAVDGNTLAVGAIGEDSNAAGANGNAF
                     NNDNNTKESGAAYVFTRSADTWTLQAYLKASNPAAGDWFGYSVALDGDTLAVGAIWED
                     SGTTGVNGDDTNDAIGDSGAAYVFVRNSNAWMQQAYIKASNPGVLDAFGYSLALERDT
                     LVVGARNEASNATGVNGDQTNNAMPYSGAAYAFMRNGATWSQQAYLKASNTGPSHAFG
                     GSLSLSGDTLAVTADGEGGATDAASGAVYVLQ"
     gene            complement(3825..4982)
                     /locus_tag="AC16BC_08675"
     CDS             complement(3825..4982)
                     /locus_tag="AC16BC_08675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3241734.1"
                     /translation="MSDASDHEPAVIALIRADIAARGPITFARFMDLALYAPGAGYYM
                     RDADRIGARGDFYTSGNLHALFGESVARQIEEMASRVGEDSFTVIEPGAGRGALAFDV
                     LNVLSRAPHRTAFRYVIVEKSPAMIARQRRLLAPWLDSGVLSWAAALPAEPIDGCVLS
                     NELVDAFPVHRVVFGGGALKEIYVDAEGEALVERLGEPSTPELAAYLERVGVSLAEGQ
                     QAEINLAAIAWMRAAGRALRRGYVLTIDYGYPAEELYAPHRARGTLLAYHRHRTNEAF
                     YERVGRQDLTAHVDFTTLAWAGRAVGLDVEGFTQQTSFLLGLGAHEAAERVLDAAADE
                     AARERALAGIRGVLDPQGMGRIFKVLIQRKGLAPGSLRGLSFGANRSLGLE"
     gene            complement(5016..5189)
                     /locus_tag="AC16BC_08680"
     CDS             complement(5016..5189)
                     /locus_tag="AC16BC_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241735.1"
                     /translation="MSVAKNLGGCLMVIVAIAGFYIAITLSLTFWALPLAIIIFFGCL
                     LLFSLALGSTDRR"
     gene            complement(5237..5659)
                     /locus_tag="AC16BC_08685"
     CDS             complement(5237..5659)
                     /locus_tag="AC16BC_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3241736.1"
                     /translation="MPKDAKTKNDTKKAPKSASSAKERELAEIRQDLETQRKTLLSDA
                     GLLIGQGLNYGAENLPDLGDQASAVTDQNFALRLKEREQRLLKKIDEALDRLTNGTFG
                     VCESCGGEISIKRLKARPVTTLCIECKTKQEEDERVRQ"
     gene            complement(5751..6026)
                     /locus_tag="AC16BC_08690"
     CDS             complement(5751..6026)
                     /locus_tag="AC16BC_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241737.1"
                     /translation="MKSYPIAAPAAPSQAPPASGTQYLVVACNVTRVVTVDGRAVGRT
                     DEVLEVPAGEHVVSLLAPPENFRPKERRILLGGTSSEKPLVVRFETR"
     gene            complement(6140..>6384)
                     /locus_tag="AC16BC_08695"
     CDS             complement(6140..>6384)
                     /locus_tag="AC16BC_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR3241738.1"
                     /translation="HAYFVGKPNPLMMRTALHQLQEHSENAIMVGDRMDTDVIAGIES
                     GMETILVLTGVTRREDVERFPYRPAQIAESVNAIEL"
BASE COUNT         1140 a         2190 c         2031 g         1023 t
ORIGIN      
        1 gtagcccagg tgcggcgtgg ccttgagaat gcgttcgcct tccagctcca gcaccacgcg
       61 gagcaccccg tgcgtgctgg ggtgctgcgg ccccatgttg agaagcagtt cctcggtccg
      121 caacaccgcg cgatcgtccg gggccggctc cggctgatgg tggatcacgc cgaagtcgtc
      181 gcggtccgcg gacgccggac ggcggtattc ggtcgtatca gccatggcgg gtccctgtgc
      241 gggtcgcgat cgtgttcatt ggctcacggt gggcccctcc cccttgggga gaaattcgaa
      301 cgtgttgcgc caccccttgc cctcggtcgg atagtccttg cgcaggggat ggccgtcgaa
      361 atcgtcggtg agcagaatcc ggcgatggtc cgggtggttg aggaactcga tccccatgag
      421 gtcgtagacc tcgcgctcca tgaaattggc tcccggccag agtggcgtca ccgactcgat
      481 ccggcagtcg tcctcgctca cgcgcgcctt gaggcggacc ctgtgccgct tctcaatgga
      541 gtagaggtga tagaccacct cgaaccgctc ctcgtcgtcc ggaaaatcca ccgacaacac
      601 gtcgctcagg aagttgaacc gccacgcggg atcgtcacgc aatgttcgac atatcgggac
      661 cagacgatcc ttgcgcaacg agatcgtcag ctcgccgaac gcttcgttga cgccggtcac
      721 cgcatccggg aaccggtcct ggacgaactg ggcgagaggg tgcacgcctt acgccttcgt
      781 caggaccctt cgctgcatga tttgttcttg cagcttgaga atgccgtcga acagcgcttc
      841 cggacgggga ggacatcccg ggacgtagat gtcgaccggg acgatttgat ccacaccctg
      901 cacgaccgag tagctgttgt aaatgttgcc cgacgtggcg cacgagccca tcgagatcac
      961 gtagcgcggc tccggcatct ggtcgtagat gcgccggatg atgggcgcca tcttgcggca
     1021 caccgtgccc gccacgatca tcaggtccga ctgccgcggc gacgcgcgaa acacgcccgc
     1081 gccgtagcgg tcgatatcgt ggcgcgcgga caccgacgtg atcatttcga tcgcgcaaca
     1141 cgcgagaccg aacgtcatgg gccacagcga cgatttgcgc gcccagttga tgacggagtc
     1201 caccttggtg gtcacgatgg tggagtccaa cccgccccct actcccattc caacgcccct
     1261 ttcttccacg cgtacacgta cccgaccacg aggatggcga tgaacaacac catctccacc
     1321 aacgcgtaca ggccgatctg cttgtacacc acggcccacg ggtacaggaa cacggcttcg
     1381 atgtcgaaaa tcacgaacaa catggtgatg acgtaatagc gcatcggaaa cgggatgcgc
     1441 gcgtcggaga acggctccac gccgctttcg tacggccgga gcttctcgcg gtacggatcg
     1501 tgcggccgga tgaaggacgc cacgaagagt tgggcgaacc cgaacaccgc cgcgatggcg
     1561 gtgaaaatca gaatcggcag gtactgctgc ggaacgtact ctccaggcac ggagcctcct
     1621 tggagaccga ttaagtcagc ccgactcgtc cgccataacc cacgtcatgg catggagcag
     1681 gggtgccata ttatcgaggg cggttttcag tgtcaagcct tttcggggat cgctcccggt
     1741 ccgccgccgg ccccggcggg gcgggccgga cgcccggcga ggggttgcgc cgacgaacgc
     1801 gcggaccttc cgcaccgacc gcccctgagc ccggtggaat agcccggaat aaatccggcg
     1861 atctccccgt cttcacggag aggagatcgc gagatctcgc gccccccgac tgtttagcgg
     1921 cgggcgtccg gtcggttcgc ggtcgaccgc atgcacagcg ccccacgaca catgccgcac
     1981 acgccccgtc cgcgggtctt tcggttgccg tcccttctcc tggccctgac gttggtggcc
     2041 tgcggcggag gcggcggggc gagcgactcg ccccccaacg cggatctcgc ggggctcacc
     2101 ctgtcgatcg ggtcgttgtc tcccgccttt gaccccgccg ccacggatta tacggcgaca
     2161 acgggctttc tttcgccctc ggtgaacgtc acgcccacgg cggcgtcatc gggcgcgacg
     2221 atcaccgtca acggagtcgc caccccgtcc ggcgtgtcgt ccgctccgat caccctggcc
     2281 gagggcgaca acgcggtgtt catcgacgtc accgcatcgg acggcaccac gaagcgatac
     2341 accgtgacaa tcgcgcaccc gaacgcggcg tcgctggtcc cgccggacta tctgaaagcg
     2401 tccaacagcg gggcctctga ccgcttcggc aaaagcgtgg ccctcgatgg cgacacgctt
     2461 gtcgtcggcg cagccttgga ggacagcgcg gccaccggcg tcaattccct ccaagggccg
     2521 agtgacaaca gtgccaccga cagcggtgcg gtgtacgtgt tcgtccgcgc caacggggcc
     2581 tggtcccaac aggcgtacat caagccatcg aacaccgggg ctggcgatca attcggcttc
     2641 agcgtggcgc tctcgggtga caccctggtc gtgggcgccc ctcgcgaaga tagtgcctcg
     2701 accggtgtcg gcggcaagca aaccaacaac caggcgtcca acagcggcgc ggtgtatgtc
     2761 tttgtccgca ccaatggcgt gtggagccaa cgggcctacc tgaaagcttc caactcggaa
     2821 gcgcaggatc actttgggtc gagcgtcgcg ctagcgggcg acaccctggt cgtgggctcg
     2881 cccggagaag acagctccgc caccggggtc aacggcgacc aaggcgacaa cgcgtccacg
     2941 gacagcggcg cggcctacgt gttcgtccgg aacgggacga cctggcaaca gcaggcctat
     3001 ctcaaagcct cgacaaccga cgcgaacccc caattcggcg actaccccga ccagttcggc
     3061 ttcagcgtcg ccgtggacgg caacacgctc gcggtgggag cgattgggga agacagcaac
     3121 gccgcgggcg ctaacggcaa tgcgttcaac aatgacaaca acacgaagga gagcggcgcg
     3181 gcgtacgtct ttacccgcag cgccgacacg tggacgctgc aggcctatct caaggcttcc
     3241 aatccggctg ccggcgattg gttcgggtac agcgtggcgc tcgacggcga taccctcgcg
     3301 gtcggcgcga tctgggaaga cagcgggacc accggggtca atggcgacga cacgaacgac
     3361 gcgatcggcg acagcggcgc ggcctacgtg tttgtccgca acagcaatgc atggatgcag
     3421 caggcctaca tcaaagcgtc gaatcccggt gtcctcgacg cgttcgggta cagcctggcg
     3481 ctggaacgcg acacgctggt ggtgggcgcc cgcaatgaag cgagcaatgc caccggggtc
     3541 aacggcgacc agacgaacaa tgcgatgcca tacagcggcg cggcgtacgc gttcatgcga
     3601 aacggcgcga cgtggagcca acaggcgtac ctgaaagcgt ccaacaccgg ccccagtcac
     3661 gcgttcgggg gcagtctctc gctctcgggc gataccctcg cggtgaccgc cgacggcgaa
     3721 ggcggcgcca ccgacgcggc cagcggcgcg gtctacgtct tgcagtagcc cggcgaagtc
     3781 cccacccgct tcacgtccgc ctcaacgagc ccgctcgcgc gggctcactc caaccccaac
     3841 gaccggttcg cgccgaacga cagcccgcgg agcgagccgg gagcaaggcc cttgcgctga
     3901 atcagcactt tgaagatccg ccccatgccc tgcggatcga ggacgccgcg gatgcccgcc
     3961 agcgcgcgct cgcgtgcggc ctcgtcggcc gcggcatcca acacgcgttc ggcggcttcg
     4021 tgcgcgccca gccccaacag aaaactcgtc tgctgagtga acccctcgac gtcgaggccg
     4081 accgctcgcc cggcccaggc cagcgtggtg aagtccacgt gcgcggtcag gtcctgacgc
     4141 cccacccgct cgtaaaacgc ttcgttggtg cggtgccggt ggtacgccag caacgtccct
     4201 ctggcgcggt gcggcgcgta cagttcctcg gccggatacc cgtagtcgat ggtcaggacg
     4261 tagccgcgcc ggagcgcgcg gcccgccgcc cgcatccacg cgatcgcggc cagattgatc
     4321 tccgcctgct gcccctcggc cagcgacacc ccgacccgtt caagatacgc ggccagctcc
     4381 ggggtggacg gctcgccgag ccgctcgacc agcgcctcgc cttcggcatc cacgtagatt
     4441 tccttgagcg cccctccgcc gaacaccacg cgatgcaccg gaaacgcgtc gaccagttcg
     4501 ttggacaaca cgcatccgtc gatcggctcc gcgggcaagg cggcggccca cgacagcacg
     4561 cctgaatcga gccacggggc gagcaggcgg cgctgccgcg cgatcatggc cgggctcttc
     4621 tccacgatca cgtagcgaaa ggccgtgcga tgcggcgcgc gcgacagcac gttgagcacg
     4681 tcgaacgcga gcgccccgcg ccccgcgccc ggctcgatca cggtgaacga gtcttcgccc
     4741 actcgcgacg ccatctcctc gatctggcgc gcgacggatt ccccgaacag cgcgtgcagg
     4801 ttgccgctgg tgtagaagtc cccgcgcgcg ccgatccggt ccgcgtcgcg catgtagtag
     4861 cccgcgcccg gcgcgtagag cgcgaggtcc atgaaccggg cgaacgtgat gggtccgcgc
     4921 gccgcgatgt cggcgcggat caacgcgatg accgcgggtt cgtggtcgga cgcgtcggac
     4981 atgtcggacc cgtcagacaa ctcgtgggcg tgaagtcacc gccggtcggt ggagcccagc
     5041 gcgagcgaga agagcaggag acagccgaag aaaatgatga tcgcgagcgg caacgcccag
     5101 aacgtcagac tcaacgtgat ggcgatgtaa aaccccgcga tcgccacgat caccatcaaa
     5161 cagccgccga gatttttggc gacgctcatg cggagggacg tccctctccg ggacgcgagg
     5221 cgcgaacgcc cgggcgctac tggcggacgc gctcgtcctc ttcctgcttg gtcttacact
     5281 cgatgcacaa cgtcgtgacc gggcgggcct tgaggcgctt gatcgaaatc tcgcccccgc
     5341 acgactcgca caccccgaac gtgccgttgg tcaggcgatc gagcgcctca tcgatcttct
     5401 tgagcaggcg ttgctcgcgt tccttgagcc gcagcgcgaa gttctgatcg gtgaccgcgc
     5461 tggcctgatc gccgaggtcc ggaaggttct ccgcgccgta gttgagcccc tgcccgatca
     5521 gcagccccgc gtcggacaac aacgtcttgc gctgcgtctc cagatcctgg cggatctcgg
     5581 cgagctcgcg ctccttcgcc gacgaagccg acttgggcgc cttcttcgtg tcgttcttgg
     5641 ttttcgcgtc cttgggcatc accaactctc aatcggtaaa gatgggaaaa caagccggga
     5701 tgttaacaaa cgttttcccg gcaagtcaaa cttcgatcgc ccggcgcgct ttaccgggtt
     5761 tcgaaacgga ccaccaacgg cttctcggac gacgtgcctc cgagcaggat ccgccgctcc
     5821 ttgggccgga aattttcggg cggagcgagc aggctcacca cgtgttcgcc ggccggaacc
     5881 tccagcacct cgtcggtgcg gcccacggcc cggccgtcca cggtgaccac gcgcgtgacg
     5941 ttgcacgcga ccacgagata ctgcgtcccg gacgccggcg gagcctgact tggcgcggcg
     6001 ggcgccgcga tgggatacga cttcatacgc acactccacg actgggcgcc ggtccctggc
     6061 gccggcgcgc tcgaactaag acggcgatgc tagacaatct cggggcctgc cgtcaagagg
     6121 gaaatcgcgc gacggaggcc tacaactcga tcgcgttgac cgactccgcg atctgcgccg
     6181 ggcgataggg gaaccgctcc acgtcctccc gccgcgtcac gccggtcagg acgaggatgg
     6241 tttccatgcc gctctcgatg cccgcgatca cgtccgtgtc catgcgatcg cccaccatga
     6301 tcgcgttctc cgagtgctcc tggagctggt gcagcgcggt ccgcatcatg agcgggttgg
     6361 gtttccccac gaagtacgcg tggc
//
LOCUS       DBNGTT010000382         2231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1624960, whole genome shotgun sequence.
ACCESSION   DBNGTT010000382 DBNGTT010000000
VERSION     DBNGTT010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1624960"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1691)
                     /gene="guaA"
                     /locus_tag="AC16BC_08700"
     CDS             complement(27..1691)
                     /gene="guaA"
                     /locus_tag="AC16BC_08700"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR3241739.1"
                     /translation="MGDVTRRGATRRGAKRPARAAKPRVAEPVAAGPSAPADRILILD
                     FGSQYTQLIARRVREGRVYCEIMPHHASFERIAAFRPKGIILSGGPASVYSRNAPSID
                     PKVLEMGLPILGICYGMQLLTKLLGGVVARAARREYGHADVMIDEPARLFDRVRAERA
                     IDGRPAFRVWMSHGDRIESLPGGFRAVAHSANSPVAAMADPQRRMWAIQFHPEVEHTP
                     QGRAILNQFVHEVCGCGSTWTMGSFRKDAVAALTRTLAGQRAICALSGGVDSAVAASL
                     VHEAIGDRLTCVFVDNGLLRKGEAAEVLATFRDSMHLTVRRVDASARFLATLKGVSDP
                     ERKRKIIGREFIRVFEAEAKKIRGANWLVQGTLYPDVIESVSVRGPSATIKTHHNVGG
                     LPSRMKFRLIEPLRELFKDEVRLLGAELGVPDNILQRHPFPGPGLAVRVLGVITPDRL
                     AILREADLIAIEEIKRAGLYRSIWQAFTVLLPVRSVGVMGDERTYDYVVAIRAVSSLD
                     GMTADWSRIPHDVLAAISSRIVNEVKGVNRVVYDISTKPPSTIEWE"
     gene            complement(1679..>2231)
                     /locus_tag="AC16BC_08705"
     CDS             complement(1679..>2231)
                     /locus_tag="AC16BC_08705"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR3241740.1"
                     /translation="RVVAGAGVPQLTAIANCAKVADRLGVPVIGDGGIKYSGDITKAI
                     AAGASTVMIGSLLAGTAESPGETILYQGRTYKLYRGMGSLGAMVKGGKDRYFQEGRPD
                     AKLVPEGIEGRVPYKGRLTDIVYQLVGGLRSGMGYCGCRTIDDLRRNGRFVRVTAAGL
                     RESHVHDVIVTQEAPNYRREWEM"
BASE COUNT          335 a          811 c          726 g          359 t
ORIGIN      
        1 ggcatgccga acccctacgc ggctggttac tcccattcga tggtgctggg cggtttcgtg
       61 gaaatatcgt acacgacccg gttcacgcct ttgacctcgt tgacgatgcg ggaggagatc
      121 gcggccagca cgtcgtgcgg gatccggctc cagtccgcgg tcatgccgtc caggctggag
      181 accgcccgga tcgccaccac gtagtcgtag gtccgctcgt cgcccatcac gcccacgctc
      241 cgcaccggca acaacacggt gaacgcctgc cagatggagc gatacagccc ggcgcgtttg
      301 atctcttcga tcgcgatcag gtcggcctcg cgcaggatcg cgagccggtc cggggtgatc
      361 acgcccagca cgcgcaccgc cagacccggc cccgggaacg ggtggcgctg caggatattg
      421 tccggcacgc ccagttccgc gcccagcaac cgcacctcgt ccttgaacaa ttcgcgcaac
      481 ggctcgatca gccggaactt catccgggac ggcaggccgc ccacgttgtg atgcgtcttg
      541 atcgtggcgg acggaccgcg caccgacacg ctctcgatca cgtcggggta caacgtgccc
      601 tggaccagcc agttcgcgcc ccggatcttc ttcgcctcgg cttcgaacac ccggatgaat
      661 tcgcgcccga tgatttttcg cttccgctcc gggtccgaca cgcccttgag tgtggcgaga
      721 aaccgcgccg aggcatccac ccgccgcacc gtgaggtgca tgctgtcgcg aaacgtggcg
      781 agcacttcgg cggcttcgcc cttgcgcagc aatccgttgt ccacgaacac gcaggtcagg
      841 cgatccccga tcgcctcgtg caccaggctc gcggccaccg cggaatccac gcccccgctc
      901 aacgcgcaga tggcgcgttg ccccgcgagc gtgcgcgtca gcgcggccac cgcgtccttg
      961 cggaacgacc ccatggtcca cgtggagccg cacccgcaca cctcgtgcac gaactgattg
     1021 aggatggccc gcccctgcgg cgtgtgttcc acctcggggt gaaactggat cgcccacatg
     1081 cggcgctgcg gatcggccat ggccgcgacc ggcgagttcg cgctgtgcgc gacggcgcgg
     1141 aatccgccgg gcagggattc gatccggtcc ccgtggctca tccagacccg aaacgccggg
     1201 cgtccgtcga tcgcgcgttc cgcccgcacg cgatcgaaca gccgcgcggg ctcgtcgatc
     1261 atcacgtcgg cgtggccgta ctcgcgccgc gcggcccgcg ccaccacccc gcccagcagc
     1321 ttggtcagca gttgcatgcc gtagcagatc cccaggatgg gcagtcccat ctccaggacc
     1381 ttggggtcga tggacggcgc gttgcgcgaa tagacgctgg ccggccctcc cgacaggatg
     1441 atgcccttgg gacggaacgc cgcgatgcgc tcgaacgagg cgtggtgcgg catgatctcg
     1501 cagtacacgc ggccttcccg gacgcgtcgc gcgatcaact gggtgtactg ggaaccgaaa
     1561 tcgaggatca ggatgcggtc ggccggagcg gacgggccgg ccgcgacggg ttcagcgacg
     1621 cggggcttgg ctgcgcgcgc gggcctcttc gcgccgcgac gagtcgctcc ccgccgggtt
     1681 acatctccca ttcgcgtcgg tagttgggag cctcttgcgt gacgatgacg tcgtggacgt
     1741 ggctctcgcg caggccggcc gcggtgaccc gcacgaagcg gccgttgcgc cgcaggtcgt
     1801 cgatggtgcg gcagccgcag taccccatgc cggaccgcag gcccccgacc agttggtaca
     1861 cgatgtccgt gagtcggccc ttgtacggca cgcggccctc gatcccctcc ggcaccagct
     1921 tggcgtcggg acggccctcc tggaagtacc ggtccttccc gcccttgacc atggcgccga
     1981 gcgagcccat gccccggtag agtttgtagg tgcggccttg atacagaatg gtctcgcccg
     2041 ggctttccgc ggtgccggcc agcaggctgc cgatcatcac ggtgctggcg cccgcggcaa
     2101 tggccttggt gatgtccccg gagtacttga tcccgccgtc cccgatcacc gggaccccga
     2161 gccggtccgc caccttcgcg cagttcgcga tcgcggtcag ctgcggcacc ccggccccgg
     2221 ccaccacgcg c
//
LOCUS       DBNGTT010000383         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1625149, whole genome shotgun sequence.
ACCESSION   DBNGTT010000383 DBNGTT010000000
VERSION     DBNGTT010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1625149"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..805)
                     /gene="cysZ"
                     /locus_tag="AC16BC_08710"
     CDS             complement(59..805)
                     /gene="cysZ"
                     /locus_tag="AC16BC_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HIR3241741.1"
                     /translation="MIAHPLIGAGYLLQGLGLIRRREMLPYVAIPVAINVAVFSASAW
                     YAVHALAPVIDRLIPAGYEWLAWVLWPLVGLAVLVLLFYVCTLLANVIAAPFNGPLAA
                     AVEAHLTGRRPGAGVGVPSAVREAGAALASEGRKLVYYVVRALPLVVLGWIPVVNLAV
                     PFLWLALNAWMLAIEYADYPMGNHRIGFPEQRATLGSKPLVAFGFGAAALLLTLIPIV
                     NFVAVPTAVAGATALWINEFRDGAAGARGG"
     gene            complement(863..1351)
                     /locus_tag="AC16BC_08715"
     CDS             complement(863..1351)
                     /locus_tag="AC16BC_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241742.1"
                     /translation="MRPFNPQHRKGPRHGPSHHRPKPNRPPDRGGSNRPQTGPPSARA
                     AAIDPFQLFCAYYLGITPDKRYAPANIHEVGRRFGVDAGVIRQVLQEHGMDPESVMNT
                     EFDLAMAQIDIQVAPEGVDRVELAKGIYQEFRDAPRKPRDWRRLLDEDARENAKIFGR
                     RD"
     gene            complement(1360..>1945)
                     /locus_tag="AC16BC_08720"
     CDS             complement(1360..>1945)
                     /locus_tag="AC16BC_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AvaI/BsoBI family type II restriction
                     endonuclease"
                     /protein_id="HIR3241743.1"
                     /translation="LGGSMRNIGGFMAQKKLTRSIIAHLNLAGKSCEWLHADTKVWAS
                     LPKDDTDVELQLRGLSWETRKGPRTVIYNLTVPIVRNNVDLCLFDCRGKKLTADTYKN
                     PSAYIALGELKGGIDPAGADEHWKTARTALDRIHKAFTKNKHRPHTFFIGAAIEAKMA
                     SEIWGLLQGGDLENAANLTDEGQIASVTRWLCSL"
BASE COUNT          373 a          611 c          605 g          356 t
ORIGIN      
        1 gacgcgagcc gtatgttggt ggagaggtgg acatggcgta tctccgggtt cggggtattt
       61 aaccaccgcg cgcgccagcc gcgccatctc gaaattcgtt gatccacaac gcggtggcgc
      121 cggctaccgc ggtcggcacc gcgacgaagt tcacgatcgg gatcagggtc agcaacaagg
      181 ccgcggcgcc gaaaccgaac gccacgagcg gtttgctgcc gagcgtcgcg cgctgttcgg
      241 gaaacccgat acggtggttc cccatcgggt agtccgcgta ttcgatcgcc agcatccagg
      301 cattgagcgc caaccacaga aacggaaccg ccaggttcac caccggaatc cacccgagca
      361 ccacgagcgg aagcgcgcgg acgacgtaat agacgagttt gcggccttcc gacgcgagcg
      421 cggctccggc ctcacgcacg gccgagggca cgccgacccc ggcaccggga cggcgtccgg
      481 tcaggtgggc ctcgaccgcc gcggccaacg gtccgttgaa cggcgcggca atcacgttgg
      541 ccagcagcgt gcagacgtaa aagagcagga ccaggacggc cagccccacc aacggccaca
      601 acacccacgc gagccactcg taccctgccg ggatgagccg gtcaatcacc ggcgcgagcg
      661 cgtgtaccgc gtaccaggcg gacgccgaga acaccgcgac attgatcgcg accggaatcg
      721 cgacgtacgg cagcatttcg cgccggcgaa tcagcccaag cccttgcagc agatagccgg
      781 cgccgatgag cgggtgggcg atcatcgcaa cggtttggtc attcggaagc cgcgaccacg
      841 cggcccacca ggatgttcgg cgtcagtcgc ggcggccgaa aatcttggcg ttttcgcggg
      901 cgtcctcgtc caggaggcgg cgccaatcgc gcggtttgcg cggcgcgtcg cgaaattcct
      961 ggtagatgcc cttggccaac tcgacgcgat ccacgccctc cggcgccacc tggatatcga
     1021 tctgcgccat ggccagatcg aactccgtgt tcatcacgga ttcgggatcc atgccgtgtt
     1081 cctggagcac ctgtcggatc acgcccgcgt ccacgccgaa cctgcggccc acttcgtgga
     1141 tgttggccgg cgcgtagcgc ttgtccgggg taatgcccag gtaatacgcg caaaaaagct
     1201 ggaacggatc gatggcggcc gcgcgcgcgg atggcggacc ggtctggggc cgattgctcc
     1261 cgcctcggtc aggggggcga ttcggcttgg gacggtggtg cgacgggcca tgcctcggcc
     1321 ccttgcggtg ctgcggatta aacggtctca tttatagcct tataggctgc atagccagcg
     1381 tgtgacggac gcaatctggc cttcatcggt tagatttgcg gcgttctcaa ggtcgccacc
     1441 ctgaagcagt ccccagatct cggaagccat cttggcttca atggcggcac cgatgaaaaa
     1501 cgtatggggc cgatgcttgt tcttggtgaa ggccttgtga attctgtcta acgccgtgcg
     1561 agccgttttc cagtgctcgt cggctccagc cggatcaata ccacctttca actcgccaag
     1621 cgcaatatat gcggaagggt tcttgtacgt gtcggccgtt agtttttttc ctcgacagtc
     1681 gaacaggcac agatcgacat tattgcgaac gatcgggact gtcaggttgt aaatgactgt
     1741 ccgtggtcct ttcctggttt cccaagacaa gccacgaagc tgaagctcta catccgtatc
     1801 gtctttcgga aggctcgccc aaactttggt atcggcgtga agccactcgc acgatttccc
     1861 agccagattg agatgagcga tgatggaacg tgtaagcttc ttctgcgcca tgaacccgcc
     1921 aatgttccgc atggagccgc ccagt
//
LOCUS       DBNGTT010000384         2933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3158276, whole genome shotgun sequence.
ACCESSION   DBNGTT010000384 DBNGTT010000000
VERSION     DBNGTT010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3158276"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /gene="rsmB"
                     /locus_tag="AC16BC_08725"
     CDS             complement(<1..1496)
                     /gene="rsmB"
                     /locus_tag="AC16BC_08725"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR3241744.1"
                     /translation="MASRLIKKKATAPAPSPAPARPASAKATSARPPSQSRRPASSSR
                     PAGRPRPGAEATTKPAREHKTASTRPPRASARPAASSREQADAPRRRATPRLPPLASL
                     ADRNRWPARRWAAELLLRADANEAFGPALLAELPSTLSPEDRGLVHELFAGVLRHRSA
                     LDWRLAACSTRRWGMMTPAVQAILRLGAYQLLFLDRIPPHAAVHETVALAKVMAQEND
                     VGFTNAVLRALERRKDELKVPSLLDRPVDHLTAEFSHPVWLVRRWLKRFGLQRTMALC
                     RANNVIPPTTLRVNTLRTTRDRLIDVLAKEGLTAEPSAVSPQGLVLAKGAPAASRAFS
                     QGWYYVQDEAAQLVGLALAPQPGERILDACAAPGGKSIHLAELMGDRGEIVAVDSSAE
                     RIDLIAQNCRRLGVTSIRTIVADLTQADAAASLGRFDRILVDAPCTALGVLRRHPEAK
                     WHKTEALIGRLVSTQRAILDAVAPLLKPGGVLVYATCSTEREENEERV"
     gene            complement(1489..2412)
                     /gene="fmt"
                     /locus_tag="AC16BC_08730"
     CDS             complement(1489..2412)
                     /gene="fmt"
                     /locus_tag="AC16BC_08730"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3241745.1"
                     /translation="MRVIFMGTPAFALPSLDALRARGEHIVAVVTQPDRPQGRGQRTA
                     APPVKVAALAAGIPVQQPAKVRQPEFLEWCRAQAPDLIVVVAFGQILPKALLDIPRLG
                     CINVHASLLPKYRGAAPIAWAIIRGERETGITTMQMDPGMDTGPMLLQRSMPIRPDDT
                     TETLGARLAELGAQTLNDTLDVLAAGRLSPIVQDSSLATLAPMLKKDHGRINWQQPAS
                     AIDALVRGLDPWPGAWTQHAGEPWRIWKTVVEPGTGEPGSVLRADAAGIAIGTGEGHL
                     VITELQAPGKRRLTVRDYLAGHAIKPGERLG"
     gene            complement(2428..>2933)
                     /gene="def"
                     /locus_tag="AC16BC_08735"
     CDS             complement(2428..>2933)
                     /gene="def"
                     /locus_tag="AC16BC_08735"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004869669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3241746.1"
                     /translation="LRIREYPEDVLKRTAEPVKNVDGRLQALIEDMFETMYAAPGLGL
                     AAPQIGEPIRLFVYDLAAREGEHDRRIIINPTIVEQEGEILEEEGCLSIPDYREVVKR
                     AARVMLKGFDRDGKEVELEAKGLEARLFQHELDHLNGVLMFDRLSPLKRDLIQRKFKK
                     RLRTEGG"
BASE COUNT          466 a         1035 c          951 g          481 t
ORIGIN      
        1 tccacgcgct cctcgttttc ttcgcgctcg gtggagcacg tggcgtatac cagcaccccg
       61 cccggtttga gcagcggcgc cacggcatcg agaatcgcgc gctgcgtgga caccaatcgt
      121 ccgatcagcg cttcggtctt gtgccacttg gcctcggggt ggcggcgcaa cacgcccaat
      181 gcggtacacg gcgcgtccac caggatgcgg tcgaaccgac cgaggctcgc cgccgcgtcg
      241 gcttgcgtca gatccgcgac gattgtgcga atactcgtca ccccgagtcg gcggcaattc
      301 tgcgcgatca agtcgattcg ttccgcgctg gagtcgacgg ccacgatctc cccgcgatca
      361 cccatcagct cggccaggtg gatggacttg cccccgggcg cggcgcacgc gtccagaata
      421 cgctcgcccg gttggggcgc gagcgccagc cccacgagct gcgcggcctc gtcctgcacg
      481 tagtaccagc cctggctgaa cgcgcgcgac gccgcgggag cgcccttcgc caacaccaaa
      541 ccttgcggcg aaaccgcgga cggctcggcg gtcaaccctt ccttggccag cacgtcgatc
      601 aaccgatccc gcgtggtccg cagcgtgttc acccgcagcg tggtcggagg aatgacgttg
      661 ttcgcccgac acaatgccat ggtgcgctgc aacccgaatc gtttgagcca ccgccgaacc
      721 agccacaccg ggtgcgaaaa ttccgcggtg agatggtcca ccggacgatc cagcaggctg
      781 ggcaccttca attcgtcctt gcgccgctcc agcgcgcgca acaccgcgtt ggtgaacccc
      841 acgtcgttct cttgcgccat caccttggcg agcgcgacgg tttcatgcac cgcggcatgc
      901 gggggaatcc gatccaggaa caggagttga tacgcgccca gacgcaggat ggcctgcacc
      961 gcgggcgtca tcatccccca ccggcgggtc gaacacgcgg ccagccgcca atccagggcc
     1021 gagcggtggc gcaacacgcc cgcgaacaac tcgtgcacca ggccgcgatc ctcgggcgac
     1081 aacgtcgagg gcaactccgc gagcagcgcg gggccgaacg cctcgttggc gtccgcgcgc
     1141 aacaggagtt ccgcggccca gcgtcgggcc ggccaccgat tgcggtccgc gagcgaggcc
     1201 agcggaggca gacgcggggt cgcccggcgg cgcggcgcgt cagcctgctc gcgtgatgac
     1261 gcggcgggcc gcgcggacgc gcgcggcggt cgggtcgagg cggtcttgtg ctcgcgcgcc
     1321 ggcttggtcg tcgcctcggc gcccgggcga ggacgccccg cgggccgcga cgaagacgcc
     1381 ggccgacgcg actgactcgg cggtcgggcg ctggtcgcct tcgcggaggc gggtcgcgcg
     1441 ggcgctgggg aaggagccgg cgccgtggct ttcttcttga tcaaccggct agccaagccg
     1501 ctctcctggt ttgatcgcat gccccgccag gtagtcgcga accgtcagac gccgcttgcc
     1561 gggcgcttgc aactcggtga tcaccagatg cccctcgcct gtcccgatcg caataccggc
     1621 ggcgtcggct cgcaggaccg agccgggctc accggtcccg ggctcgacaa cggttttcca
     1681 aatacgccac ggctctccgg cgtgctgcgt ccacgctccg ggccacgggt ccaagccccg
     1741 caccagcgcg tcaattgcgg acgcgggttg ctgccaattg atccggccgt ggtccttttt
     1801 gagcatgggc gccaacgtgg ccaatgaaga gtcctggact atcggcgaca atcgtccggc
     1861 cgcgagcaca tccagcgtgt cgttgagcgt ctgcgccccc aattccgcaa ggcgcgcgcc
     1921 cagtgtctcc gtcgtatcat ccgggcggat cggcatcgat cgctgcagca acatgggccc
     1981 ggtgtccatg cccgggtcca tctgcatggt ggtgattccg gtctcgcgct cgccacggat
     2041 aatcgcccac gcaatgggcg cggcgccccg gtacttgggc aacagcgagg catggacgtt
     2101 gatacagccc aaacgcggga tgtccaacaa cgccttgggc aggatctgtc cgaacgccac
     2161 caccacgatc aggtccggcg cctgggcacg gcaccactcc aggaactcgg gttgtcgaac
     2221 cttggccggc tgctgcaccg gaatgcccgc cgcgagcgcc gcgactttca ccggcggcgc
     2281 agccgtccgc tgcccgcggc cttgcggccg atccggctgc gtgaccacgg ccacgatgtg
     2341 ctcgcctcgt gcccgcagcg cgtccaaaga cggcagcgcg aacgcgggcg tgcccatgaa
     2401 gatcacgcgc atggtgaaat tggacgatta cccgccttcg gtgcgaaggc gttttttgaa
     2461 cttacgctga atcaggtcgc gcttcaacgg gctgaggcgg tcgaacatca gcaccccgtt
     2521 gaggtgatcc agttcgtgct gaaagagccg cgcctcaagg cccttggctt ccagttcgac
     2581 ttccttgccg tcgcgatcaa agcccttgag catgacccgg gccgcgcgtt tgaccacctc
     2641 gcgatagtcc gggatcgaga ggcagccctc ttcctccaag atctcgccct cttgttccac
     2701 gatggtgggg ttgatgatga tgcggcgatc gtgttcgccc tcgcgcgcgg ccagatcgta
     2761 tacgaacaag cgaatcggtt ccccgatctg cggcgcggcc aacccaagcc ccggcgccgc
     2821 gtacatggtc tcgaacatgt cctcgatcag cgcctgcagc ctgccgtcca cgttcttgac
     2881 gggctccgcc gtgcgcttga gcacgtcttc cgggtattcg cgaatgcgta gga
//
LOCUS       DBNGTT010000385         1712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_475403, whole genome shotgun sequence.
ACCESSION   DBNGTT010000385 DBNGTT010000000
VERSION     DBNGTT010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_475403"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1160
                     /locus_tag="AC16BC_08740"
     CDS             129..1160
                     /locus_tag="AC16BC_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3241747.1"
                     /translation="MRQSILGVAFALGWGLASTPVAEAAPTTVRVAHFPNITHAQALV
                     GRADGSFSRALGAETTIDWKLFSAGPAVIQALFAGELDLAYLGTGPAIVGFVKSRGEA
                     LRIIAGAADGGAALVVRDESGITGPQDFHGKRVATPQLGNTQDVALRGWLAAHGLQVM
                     ERGGDVRVIPMANAEQLSMFRRGEIDAAWTAEPWVSRLVHEAGGRVLLDERDEWKPLT
                     GGKFATTVLVVHPRFLAAHPELVARWITAHVELTSRISAQPDEAKRLTNEELRRLTGK
                     PLPEAVLNEAWGRVAFTTDPIVPSIVQMARWAFDQGFLGRREPDLSRIVDGRWLADAT
                     PVSEPRSAP"
     gene            1157..>1712
                     /locus_tag="AC16BC_08745"
     CDS             1157..>1712
                     /locus_tag="AC16BC_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3241748.1"
                     /translation="MSPVTPHAVVDVRRQQCPYPLLMIKKALDGLQEGQILEAQTDHL
                     PTARETTPRFCRDMGYVFDVIEEPGLWRLIITKGNEAPKPVSVTLEGVGKWYPSSGGQ
                     VTALENINLQILEGEFVCIVGPSGCGKSTLLNLVAGLEFPDAGRVTIKGHAVTGAGAD
                     RVVVFQEGALFPWLTVRQNVEFGLK"
BASE COUNT          279 a          509 c          618 g          306 t
ORIGIN      
        1 ggtcggactg acggcggggt gaccctgcgg aggtctggtc cgccggctct cccgcccagg
       61 gtgcctcgcg tatggtccac gcacggcgtt cttgacggcc tttccgccga tccattattc
      121 tgactccaat gcgtcaatcc attctcggtg tggcgttcgc gctcggatgg gggctggcgt
      181 caacgccagt tgcggaggcc gcccccacca cggtccgcgt cgcccacttt cccaatatca
      241 cgcatgctca ggcgttggtc ggccgtgcgg acgggtcgtt ttcgcgggcg ttgggcgcgg
      301 agacaaccat cgactggaag ctgttcagcg cgggaccggc cgtgattcag gcgctgtttg
      361 ccggtgagct tgatctggcg tacctcggaa ccggaccggc catcgtgggc ttcgtgaaat
      421 cccgtgggga ggcgttgcgg attattgccg gggccgcgga cgggggcgcc gcgctggtgg
      481 ttcgagatga atccgggatc accgggccgc aggattttca cggcaagcgc gtggccacgc
      541 cgcaacttgg gaacacgcag gatgtcgcgc tgcgcggttg gctcgcggcg catggcttgc
      601 aagtgatgga acggggcggc gacgtgcggg tgataccgat ggccaacgcg gagcaactct
      661 ccatgttccg acggggcgaa atcgacgcgg cgtggaccgc ggagccgtgg gtctctcgtc
      721 tcgtgcacga agccggtggc cgggtgctgc ttgatgaacg cgacgaatgg aaaccgttga
      781 ccggcgggaa attcgccacc accgttctgg tggtgcaccc gcggtttctc gcggcgcatc
      841 ccgaactggt agcccgatgg atcaccgcgc atgtggaact gacgtcgcgc atcagcgcgc
      901 agccagatga agccaaacgc ctgacgaacg aggaactcag acggttgacc gggaagccgc
      961 tgcccgaggc ggtcctgaat gaggcatggg gccgggtcgc gttcacaacc gaccccatcg
     1021 tgccgtccat cgtgcaaatg gcccgctggg cctttgatca gggattcctc ggacggcgcg
     1081 aacccgattt gtcccggatc gtggacgggc gttggctggc agacgcgacg ccggtttccg
     1141 agccgcggag cgcgccatga gtcccgtgac tccgcacgcg gtggtcgacg ttcgtcggca
     1201 gcagtgtccg tacccgctgt tgatgatcaa aaaggcgctc gacggcttgc aggagggcca
     1261 gatcctggag gcgcagaccg accacctgcc gaccgcgcgg gaaaccacgc cgcgattttg
     1321 ccgggacatg gggtacgtgt tcgacgtgat cgaggagccc gggttgtggc gattgatcat
     1381 caccaagggc aacgaggctc ccaaaccggt gagcgtcacg ctcgaggggg tcggcaagtg
     1441 gtatccgtcg agcggggggc aagtcaccgc gctggaaaac atcaatctcc agattctcga
     1501 aggggagttc gtgtgtatcg tggggccctc ggggtgcggg aaatccacgc tgctcaacct
     1561 ggtcgcgggc ctggagtttc cggatgccgg gcgggtgacg attaaggggc acgcggtgac
     1621 cggcgcgggc gcggatcgcg tggtggtgtt ccaggagggc gcgctgttcc cgtggctgac
     1681 cgttcggcag aacgtcgaat tcgggctcaa ag
//
LOCUS       DBNGTT010000386         2995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1880879, whole genome shotgun sequence.
ACCESSION   DBNGTT010000386 DBNGTT010000000
VERSION     DBNGTT010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1880879"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1168
                     /locus_tag="AC16BC_08750"
     CDS             134..1168
                     /locus_tag="AC16BC_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR3241749.1"
                     /translation="MSGLTPRRTMITLLILLGLLALTGFTALLLGTERVPFGRLVRIV
                     TGLPASDAPADVSDTIILQIRGPRVLLAGLVGAVLGLTGAVLQSLLRNPLAEPHLIGV
                     SSGAALGVMIGSIWPMEGLIQGLTSAWLFAFAGGMAALVMLYRLATVNGRIRPETLVL
                     AGVVLNGTLAAVIMFLTSYLDPGRVFGRMVWLMGSLGSVESAVLWLTAPVLLAGMAWL
                     CLQGRPLNLMTLGADTAQSLGLQPDRVIRASLVVSASMIGLVVSLSGMIGFVGILTPH
                     ILRRLIGTDYRLLLPASALGGALFLIVADTVARVALAPTELPVGVVTALCGGPFFLWL
                     LAQRRREVAG"
     gene            1165..1965
                     /locus_tag="AC16BC_08755"
     CDS             1165..1965
                     /locus_tag="AC16BC_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3241750.1"
                     /translation="MTSPRTAQPIFRLDRVTVAYGSNAALREATLDVAAGAMTAVIGS
                     NGSGKSTALRVLAGLIEPTSGTVAFDGAPLQSLTPRERARRIAYVPQDTAIPFEFSVR
                     EIVAMGRSPYISAWGFESPDDQAAIDHAVALMDLGPLVARSILDVSGGERQRAVIARA
                     LAQRPSVLLLDEPGANLDMKHQIALHALLARLNREEGLTTVTVSHDLNAVAGSDRVIA
                     LKNGRIHAMGPPTTVLTEAVVADVFTCRALIDAHPRTGRPRVTMEWIE"
     gene            1962..2699
                     /locus_tag="AC16BC_08760"
     CDS             1962..2699
                     /locus_tag="AC16BC_08760"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3241751.1"
                     /translation="MSRPGFLRWFGWSAAAHLAVGSALWIGLPKADPTNLPAPDAAVT
                     MVALIEAPTEDPAAPAPRRVARHASRPVTPIEHAPPAATEDHPRPEPIFDAALAAPVP
                     TEPDTLVALSDPAPDSTEQARLQLPLPEAPQQVSDEDGSIPAEISANAGVPTDAYRPL
                     VLAMLERAKRYPLVADRWRLEGTVDIAFTIHPDGRLSDPELIASSTHPILDDAALAIV
                     RRVRYVPPPPSQASIRFSALIRYQVDQ"
     gene            2731..>2995
                     /locus_tag="AC16BC_08765"
     CDS             2731..>2995
                     /locus_tag="AC16BC_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3241752.1"
                     /translation="MRRAFVLLVLSILALPIVHASAQPTAKPDIVVHGTKTGTPIEQL
                     TGAVTVIDEAAIKEKRVPSVLEVLREVDGLDVVQSGGGGGVWWV"
BASE COUNT          415 a         1053 c          984 g          543 t
ORIGIN      
        1 cggcggtcaa cgccggccgg atgtacgcca tcccccgcga tctcgtcaac cggcccggac
       61 cgcgaatcat cgacgccatc gaattcatcg cgtccacgct ccatcccccg gatcagaacg
      121 tggagcatgc gccgtgagcg ggttgacccc tcgccgcacc atgatcacgc tgctgatcct
      181 gctgggcctg ttggccctga ccggattcac cgcactgctg ctgggcaccg agcgggtgcc
      241 gttcggtcgc ctggtccgga tcgtgaccgg gttgcccgcg tccgatgcgc ccgccgacgt
      301 gagcgacacc attattctcc aaatccgcgg cccgcgcgtg ttgttggccg ggctcgtcgg
      361 ggcggtgctg gggctgacgg gcgcggtgct gcaatcgttg ttgcgaaacc ccttggccga
      421 gccgcatttg atcggcgtct ccagcggcgc cgccttgggc gtcatgatcg gtagtatctg
      481 gcccatggag ggactgatcc aggggctcac ctccgcgtgg ctgttcgcgt tcgccggcgg
      541 catggccgcg ctcgtgatgc tctatcgcct cgccacggtc aacggccgca tccgacccga
      601 aaccctggtg ctcgcgggcg tggtcctcaa cgggaccctg gccgcggtga tcatgttcct
      661 gaccagttat ctggatcccg gacgcgtgtt cggacgcatg gtgtggctca tgggcagcct
      721 gggatcggtc gagtccgcgg tgttgtggct caccgccccg gttctgctgg ccggcatggc
      781 ttggctctgc ctgcagggcc gcccgctcaa tctcatgacg ttgggcgcgg acaccgcgca
      841 gtcgctgggt ttgcaaccgg atcgcgtcat tcgcgcgagc ctggtggttt ccgcttcaat
      901 gatcgggctc gtggtctcgc tgagcggcat gatcgggttc gtgggcattc tcacgcccca
      961 catcctgcga cgcttgatcg ggaccgacta ccgcctgctg ctgcccgcgt ccgcgctcgg
     1021 gggcgcgctg tttctgatcg tggccgacac cgtggcgcgg gtcgcgctcg cgcccacgga
     1081 gttgccggtc ggcgtcgtga ccgcgttgtg cggcggtcct tttttcctct ggttgttggc
     1141 gcaacgccga cgcgaggtcg cgggatgacg agtccgcgca ccgcgcagcc gatcttccgc
     1201 ctcgatcgcg tcaccgtcgc gtacggctcg aacgccgcgc ttcgtgaggc cacgctcgac
     1261 gtcgcggccg gcgccatgac cgcggtgatc ggctccaacg ggtcgggcaa gagcacggcg
     1321 ctgcgcgtgc tcgcggggtt gatcgagccg acgtcgggaa ccgtcgcctt cgacggcgcg
     1381 ccgcttcagt ccctgacgcc gcgcgagcgc gcccgccgga tcgcgtacgt gccgcaggat
     1441 acggccatcc cgttcgaatt ttcggtgcgc gagatcgtgg ccatgggccg gtccccctac
     1501 atctccgcgt ggggcttcga gtcgccggac gatcaggccg cgatcgacca cgccgtggcg
     1561 ctgatggacc tggggccctt ggtggctcgc tcgatcctgg acgtgtcggg cggcgagcgc
     1621 caacgcgcgg tcatcgcccg cgccctggcc caacgcccgt ccgtgctgtt gttggacgag
     1681 cccggcgcga atctggacat gaagcaccag atcgcgctgc acgcgctgtt ggcgcgcctg
     1741 aaccgcgagg aggggttgac cacggtcacg gtctcgcacg acctcaacgc cgtggcgggt
     1801 tcggatcgcg tcattgcgct caagaacggt cgaattcacg cgatgggacc gcccacgacc
     1861 gtgctcaccg aagccgtggt cgccgacgtg tttacctgcc gcgccttgat cgacgcgcat
     1921 ccgcgcacgg gccgaccccg cgtcaccatg gagtggatcg agtgagccgt ccgggattcc
     1981 tccgttggtt cgggtggtcc gcggccgccc accttgccgt gggctcggcg ctctggatcg
     2041 gattgcccaa ggcggatccg accaacctcc ccgcgccgga cgccgcggtc acgatggtcg
     2101 ccctgatcga ggcgccgacg gaagaccccg cagctcctgc gccgcggcgc gtcgctcgcc
     2161 acgcatcacg cccggtgact ccgatcgaac acgccccccc ggccgcgacc gaggaccatc
     2221 ctcgcccgga gccgatcttt gacgcggcgc ttgccgcgcc ggttcccacg gaaccggata
     2281 ccctggttgc cctgagcgat cccgcgcccg attcgaccga gcaggcacgc ctgcagcttc
     2341 cgctccccga ggctccccag caagtctccg acgaagacgg atctattccc gccgaaatct
     2401 ccgcgaacgc cggagtcccg accgacgcct accggccgct ggtgctggcc atgctcgaac
     2461 gagccaagcg gtatccgctg gtcgcggatc ggtggcgcct cgaaggcacg gtggacatcg
     2521 cgtttacgat tcacccggac ggacgcctct ccgaccctga actgatcgcc tcgtccacgc
     2581 atcccatatt ggatgacgcg gccttggcca tcgtccgccg cgtgcggtat gtccctccgc
     2641 cgccgtccca agcgtccatc cgattttcag ccctgattcg ataccaagtt gaccaatgac
     2701 gtccaccgat ttcacccaag gagcctcatc atgcgtcgtg cattcgtcct acttgtcctg
     2761 agcattctcg cgctacccat cgtccacgcg tcggctcaac cgacggcgaa gccggacatc
     2821 gtggtgcacg gcaccaagac cggcactccg atcgaacagc tcaccggcgc ggtgacggtt
     2881 attgacgagg ccgcgatcaa ggagaagcgg gtcccgagcg tgctggaagt cctccgagag
     2941 gtcgacggat tggacgtggt gcagagcggt ggtgggggtg gggtttggtg ggtgt
//
LOCUS       DBNGTT010000387         4094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2137501, whole genome shotgun sequence.
ACCESSION   DBNGTT010000387 DBNGTT010000000
VERSION     DBNGTT010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2137501"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..207
                     /locus_tag="AC16BC_08770"
     tRNA            131..207
                     /locus_tag="AC16BC_08770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:165..167,aa:Arg,seq:tct)
     gene            complement(226..1704)
                     /locus_tag="AC16BC_08775"
     CDS             complement(226..1704)
                     /locus_tag="AC16BC_08775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3241753.1"
                     /translation="MPPPTPSHSSLDEPVERDLRQIERREWQLWGVALTLLVASVAVT
                     VMSSYLLLQSDSSSPDLVRLLTSRGLPGLVVLVGLFCVYVFHTRVTFGRMRRLFEKQA
                     MRDALTGLFNRRYFSERVGNDLLHTEHDKGMFAVLLCDLDHFKKVNDSRGHQTGDDVL
                     KEVGKAILEATRGADLVVRWGGDEILVVLTRTTRDGVLTAADRIRKGVTLVAQQREIP
                     LDVSIGAALYPEHGRTVEELIRLADRALYIAKHGGDKIHIGEEEYRLDDHTVGTVFQP
                     VVDRTTGDILGYEALSRDPAGKVGIGELFRRYQAVGHLHELKCLCLRLQLQAAARLGL
                     QRVFINVDFAVLDHVRVIPKPPHTEVVLEISESEALHDVERHLNTTKTWRAAGYKFAI
                     DDFGAGFISLPFVARLIPDYIKLDRATIVQAGASRQFLTFLRDLILALRNYSKEGIIA
                     EGIETPDELQVVRDLGISPVQGFLVGKPQELHADTLRERRAA"
     gene            1840..2955
                     /locus_tag="AC16BC_08780"
     CDS             1840..2955
                     /locus_tag="AC16BC_08780"
                     /inference="COORDINATES: protein motif:HMM:NF019135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR3241754.1"
                     /translation="MTGALATRRLTEAVASLSRAGWLLTVGILVCSCTKESPLQYRVG
                     ESFETAADVFVRSLALEGPYLWVATSDGVIKVERASGRKVGHYTTQTGLMVNYAFTVA
                     VDPRGTTWFGTNSGGLARVDGERVTTFHVRDGLADPWVYDIAYQRDGTMWAGTWNGVS
                     RYDGKAFTSYHVEDGLINRWVYAVAVDRDDSVWFGTEGGVSRFDGATWTSWTHRDGLG
                     APNTAQLKSSDNTGLGALTTGNIHRHDLTALDPEGRETYNENYVLSLLIDPRGVKWIG
                     TWGGGVARFDGRTWTNYTTQDGLAGNVVYAIAQDPTGRLWFGTNHGVCTYDGRTWATV
                     SPAQRYAAGDVYAVVVDTDRSVWIGYKGGVAHITGRS"
     gene            3042..>4094
                     /gene="rimO"
                     /locus_tag="AC16BC_08785"
     CDS             3042..>4094
                     /gene="rimO"
                     /locus_tag="AC16BC_08785"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR3241755.1"
                     /translation="MTLGCPKNQVDSEVMLGQLAEAGLRAVEDPGRADVVIVNTCGFI
                     DTAKEESINTIIELGELKKSGSCRALIATGCLTQRYQGELLKELPELDAIVGTGDFPR
                     IAEIAQSQLGQKSRSAREWFEHPTFLYDAATPRRRLGPRHWAYVKISEGCDKRCSFCA
                     IPSFRGDLASRSVDSIVAEVRQLAADGVREINLIAQDLPAFGMDHGRKGELIPLIRAL
                     AKIDGVRWIRPMYLYPHKLPKGLIDVFVEEPKVVPYVEMPIQHIDDAILRAMNRGGTS
                     AEIRRTLDAFRDRVPGVAIRTSFIVGFPGETDAAFDRLVSFVEEQRFDRIAVFTYSME
                     EGTTAAPLGDPIAPEVK"
BASE COUNT          753 a         1215 c         1368 g          758 t
ORIGIN      
        1 cgctggttca aatccagtcg ccccgatttt ttaaagacga aggggtgagt gggcgatggg
       61 gagaatgggc gaaagtgctt ttcaccccct cacccgctct ccccgtctct ccttcgtctt
      121 ttcccgacac gcgcctgtag ctcagtcgga tagagcaaca gccttctaag ctgtgggtcg
      181 ctggttcgat tccagccagg cgcaccactt tccaggcgag gcgtctcacg ccgcgcggcg
      241 ctcgcgaagg gtgtcggcgt gcagttcctg cggtttgccc accaggaagc cttggaccgg
      301 gctgattccc aaatcgcgca cgacttgaag ctcgtcgggc gtttcaatgc cttccgcgat
      361 gatgccttcc ttcgagtagt tgcgcagcgc caggatgagg tcgcggagaa acgtcaggaa
      421 ttgccgggac gctccggcct ggacgatcgt ggcgcgatcc agtttgatgt agtcgggaat
      481 gaggcgcgcc acgaatggca gcgaaataaa cccggcgccg aaatcatcga tcgcaaactt
      541 gtagcccgcg gcgcgccagg ttttggtcgt gttgagatgg cgctccacgt cgtgtaacgc
      601 ttcggattcc gagatttcca ggaccacttc cgtatgtggc ggcttcggga tgacgcggac
      661 gtgatccagt accgcgaaat ccacgttgat gaaaacgcgt tgaagcccga gccgggcagc
      721 ggcttgaagt tgaagccgga ggcacaaaca tttgagttcg tgcaggtggc ccaccgcttg
      781 gtagcggcgg aacagctcgc cgatccccac cttgccggcg gggtctcggc tgagggcttc
      841 atagcccaga atgtcgcccg tggttcgatc caccacgggt tgaaacacgg tgccgacggt
      901 gtggtcatcc aagcggtatt cttcttcccc gatatgaatt ttgtcccctc cgtgtttcgc
      961 gatgtagagc gcccgatccg cgaggcggat gagttcttcg acggtccggc catgctcggg
     1021 gtacaacgcg gcgccgatgc tcacgtccaa gggaatctcg cgctgttgcg cgacgagcgt
     1081 gacccctttt cgaatacggt cggccgcggt taatacgcca tcgcgggtgg tccgtgtgag
     1141 cacgaccagg atttcatcgc cgccccaccg caccacgaga tccgcgccac gagtggcttc
     1201 cagaatggct tttccgacct ccttgaggac gtcgtctccc gtctggtgac ctcgggagtc
     1261 gttcactttc ttgaagtggt cgagatcgca cagcagcacg gcgaacatcc ccttgtcgtg
     1321 ctcggtgtgg agcaggtcgt tgccgactcg ctcgctgaaa tatcggcgat taaagagccc
     1381 ggtgagggcg tcacgcatgg cttgtttttc gaagagccgt cgcatccgcc cgaacgtgac
     1441 ccgtgtgtgg aagacgtaga cgcaaaacag cccgacgagg acgaccaatc ccggcaaacc
     1501 tcgggacgtg aggagccgaa ccaagtcagg actgctcgag tcggattgca gcaagagata
     1561 ggatgacatc acggtgaccg cgacggacgc gaccaggagg gtcagcgcga cgccccataa
     1621 ctgccactcg cggcgctcga tctgacggag gtcgcgctcg accggttcgt ccaaggacga
     1681 gtgtgacggc gtgggcgggg gcatgagccc tccaaggata cccagggacc gtggcgggtc
     1741 aaggcggagc cctcggcaag gcgcttcctt gctcaacccg ggacggctgt gttaaggtct
     1801 ggtgcgtgaa ggattatccc taatatggat gtttggccgg tgacgggggc gcttgcgacc
     1861 aggcgcttaa ccgaggccgt cgcgtcgttg agtcgcgcgg gctggctcct gacggtgggt
     1921 attttggtgt gttcctgcac gaaagagtcg ccgctccagt atcgcgtggg cgaaagcttt
     1981 gaaaccgcgg cggacgtctt cgttcgatcc ctcgcgctcg agggccccta tctgtgggtg
     2041 gcgacgtcgg acggggtgat caaggtcgag cgcgcgagcg ggcgcaaggt cggtcactac
     2101 acgacccaga ccgggttgat ggtcaattat gcgtttacgg tggccgtgga cccgcgcggg
     2161 acgacgtggt tcgggaccaa cagcggcggg ttggcgcggg tggacggcga gcgcgtcacg
     2221 acgtttcacg tccgggatgg cttggcggac ccgtgggtct acgatatcgc gtaccaacgg
     2281 gacggcacca tgtgggccgg cacctggaac ggcgtcagcc gatatgacgg gaaggcgttc
     2341 acctcgtatc acgtggagga cgggctgatc aaccggtggg tgtacgcggt cgcggtcgat
     2401 cgcgacgatt cggtgtggtt cgggaccgaa gggggcgtca gccgcttcga cggcgcgacc
     2461 tggacgagct ggacgcatcg cgacggattg ggcgctccca ataccgcgca gttgaagtcc
     2521 agcgacaata cggggctcgg cgcgttgacg accggcaata tccaccgaca cgacctcacc
     2581 gcgctggacc cggagggccg cgagacgtac aacgaaaact acgtgctgtc cctgctgatc
     2641 gatccacgcg gcgtcaagtg gatcggcacg tggggcggcg gcgtggcgcg gtttgacggg
     2701 cgaacctgga cgaactacac gactcaggac ggcttggccg gcaacgtcgt gtacgcgatc
     2761 gcccaggatc ccacgggtcg tctgtggttc ggcaccaatc acggcgtgtg cacgtacgac
     2821 gggcggacct gggccaccgt gtcaccggct cagcgctacg cggccggcga cgtgtatgcc
     2881 gtggtcgtgg ataccgaccg ctcggtgtgg atcgggtata agggcggggt ggcgcacatt
     2941 accgggcggt cgtagccgcg tccccgacaa ggacctcagt gcgaaaaaca cgaccaacac
     3001 caacacgccc tcgcgccgcg aagcaaccca cggtcgcttt ggtgaccctc gggtgcccca
     3061 agaatcaggt ggactccgag gtgatgttgg gacaactcgc cgaagcgggt ctgcgcgcgg
     3121 tggaagaccc cggtcgagcg gacgtagtga tcgtcaacac gtgcgggttc atcgacacgg
     3181 cgaaagaaga gtcgatcaac acgatcattg agctgggcga actcaaaaag agcggatcgt
     3241 gccgcgcgtt gatcgcgacc ggatgcctga cccagcgcta ccaaggcgaa ctgctcaagg
     3301 agttgccgga attggacgcg atcgtcggga ccggcgactt tccgcgcatt gccgagatcg
     3361 cgcagtctca attgggccaa aagtcccgtt ccgcgcgcga gtggttcgag caccccacgt
     3421 tcctgtacga cgccgccacg ccgcgccgtc gcctcgggcc tcggcactgg gcgtacgtca
     3481 agatttccga ggggtgtgat aagcggtgct cgttctgcgc gatccccagt ttccgaggcg
     3541 atttggccag ccggagcgtg gactccatcg tggcggaggt gcgtcaactg gcggccgacg
     3601 gcgtgcgcga gatcaacttg atcgcccagg atctccccgc gtttggcatg gaccacggcc
     3661 gcaaggggga attgatcccc ttgatccgcg cgctggccaa gatcgacggc gtgcgatgga
     3721 ttcggcccat gtacctgtat ccacacaagc ttcccaaggg cttgatcgac gtgttcgtgg
     3781 aggagcccaa ggtcgtcccg tacgtggaaa tgccgatcca gcatatcgat gacgcgatct
     3841 tgcgcgcgat gaatcgcggc gggaccagcg cggaaattcg acgcacgctc gacgcgtttc
     3901 gtgaccgcgt gccaggcgtg gcgatcagga cctcgttcat cgtggggttt ccgggagaga
     3961 ccgacgcggc gttcgatcga ctcgtgtcgt tcgttgaaga gcaacggttc gaccggatcg
     4021 cggtcttcac ctattcgatg gaagaaggca cgaccgcggc cccgttgggc gacccgattg
     4081 cacccgaggt caag
//
LOCUS       DBNGTT010000388         2534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2519177, whole genome shotgun sequence.
ACCESSION   DBNGTT010000388 DBNGTT010000000
VERSION     DBNGTT010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2519177"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC16BC_08790"
     CDS             complement(<1..183)
                     /locus_tag="AC16BC_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241756.1"
                     /translation="MTERVCRARPRAAANMIGAAVVAATVICSTAFATMPSAPVTVTI
                     ESAGPVAVDVDLTITLL"
     gene            complement(299..1126)
                     /locus_tag="AC16BC_08795"
     CDS             complement(299..1126)
                     /locus_tag="AC16BC_08795"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3241757.1"
                     /translation="MTTQTSEVHGEHGGAVALALSGGAARAAGHVGVLCALADAGVPI
                     AGVVGTSAGAFVGALFASGRYSADELEEVARGLRWTDVLRPALSMRGFWTSEQVGTAL
                     RRLIGGLRFSDLRRPFAAVACDLRTGEQVLLQEGDVCCAVQASCSLPVFFTPTIVGDR
                     VLVDGGAVSQLPVLAARAQFPGSPIVGVDVNYRATETARLSNMVSVGVQVVSLFARQN
                     AARERAEADLMIDVDAAGVALYDLAKRDLMIVRGRAAAKAALANNPHIARTAKIPPR"
     gene            1199..>2534
                     /locus_tag="AC16BC_08800"
     CDS             1199..>2534
                     /locus_tag="AC16BC_08800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3241758.1"
                     /translation="MAVRPPLGRHRLVRRLVLPYALLIIGTLAVSGWFFYGAAQDALD
                     QELTKRLLTVAQTVRSVITPRYLTQLRPSSEGTTLYALLLADLKRVQQASKASHIYVL
                     DPQHRMLLDGDERTPIGREFLSVKLDAADLADVWKGRSAASILYRGQDGKFYKSGYAP
                     LVDQDGTVVAIVGVEAGADFFEALTVTRRQVALTVAVSLLILLSIGWWLSRSILKPLD
                     VLVSAMDRVGKGEPPAKVNVLNQDEIGYLGRRFNEMVDALAEKDRLLTELYQQERQRA
                     DRHRGYSDFLLRSMTTGVIGVDFAGRLTSINPAALDILDLSTEDMGEELDSLLERDHS
                     IMILLESTLAGQAVQTRQELRLQTRAGRQKWVGVHTAPLHDHDGILIGASALLTDLTA
                     VRKLQDEIRRKDRLAAIGQLSAGIAHEIRNPLAAIEGFSELLARRVPDEGARV"
BASE COUNT          483 a          865 c          747 g          439 t
ORIGIN      
        1 gagcagcgtg atggtcagat ccacatcgac cgccaccggc cccgcggact cgatcgtcac
       61 tgtgaccgga gccgagggca tggtcgcgaa ggcggtggaa caaatcaccg tcgctgcgac
      121 aaccgccgcg ccgatcatgt tcgccgcggc acgcggacgc gcgcggcata cccgctctgt
      181 catggccgcc cccacaagca cctagacacc ttttttttga ggtttattcg gccgctcgcc
      241 atgagggggc catatctcgg ccatcccgca agcgtccggg gaccagaagt ggttcgattc
      301 agcgcggtgg gattttcgcg gttcttgcga tatgggggtt gttcgccaac gccgccttgg
      361 ccgcggcacg tccacgaacg atcatcagat ctcgtttcgc gaggtcatac agagccaccc
      421 cggcggcgtc gacgtcgatc attaggtcgg cctccgcccg ctctcgagcc gcgttctgac
      481 gcgcaaagag cgacaccacc tggacgccga ccgacaccat attggacaac cgcgccgttt
      541 ccgtcgcccg ataattcacg tccactccca cgatcggact ccccggaaat tgcgcgcggg
      601 ctgccagaac cgggagctgg ctcaccgctc cgccgtcgac caacacccgg tcgccgacaa
      661 tcgtcggggt gaagaacacg ggcagactgc aactggcctg caccgcgcaa cagacgtcgc
      721 cctcttgcag gagcacctgt tccccggtcc gcagatcaca cgcgaccgcg gcgaacggcc
      781 ttcgcaggtc ggagaaccga agcccgccga ttaatcgacg aagcgcggtg ccgacctgct
      841 cgctggtcca gaatcctcgc atcgagagcg ccggccggag cacatcggtc catcggagcc
      901 ctctcgcgac ctcctccaat tcgtccgccg aataccgtcc cgaggcaaat aaggcgccca
      961 caaacgcccc ggcgctggtc ccgaccacgc ccgcaatcgg aacccccgcg tccgccaatg
     1021 cacagagcac gcccacgtgc cctgcggcgc gcgccgcgcc gccgctcagc gcgagcgcca
     1081 ccgccccgcc atgctcgcca tggacttcgg aggtttgtgt cgtcatactc agcggcatac
     1141 tatatcccac atccgcggtt gacttcccgc agagacatcc cctaagctga tggcgtcaat
     1201 ggcagtccgt cctcccctcg gacgccaccg cctggtccgc cgtctggttc ttccgtacgc
     1261 gctcctgatt atcggcacct tggcggtgag cggttggttc ttctacggcg cggcgcagga
     1321 cgcgctcgac caagaattaa ccaaacgcct gctgaccgtg gcgcagacgg tccggtcagt
     1381 gatcactccg cggtatctca cgcaattgcg tccgagcagc gaaggtacca ccttatacgc
     1441 gctcctcctc gccgatctca agcgggtcca gcaagcctcg aaggccagcc atatctatgt
     1501 tttggacccc caacatcgaa tgttactgga cggcgatgaa cgcaccccga tcggccgaga
     1561 gtttttgtcg gtgaaactgg acgcggccga tctcgccgat gtctggaaag gccgatccgc
     1621 tgcgtcgatt ttgtaccgcg gacaggacgg aaaattctat aaatcaggct acgcgccgct
     1681 ggtcgaccag gacgggaccg tcgtcgcgat cgtgggcgtg gaagcgggcg cggacttttt
     1741 cgaggcgctc acggtcacgc gacgtcaggt ggccctaacc gtcgcggtca gcctgctcat
     1801 cctcctttcc atcggctggt ggctctcccg atcgatcctc aaaccattag acgtcctggt
     1861 ttccgcaatg gaccgcgtcg gcaaaggcga acctccggcc aaggtcaacg tgctgaatca
     1921 agatgaaatc ggctatttag gtcgccggtt caacgaaatg gtggacgcgt tggcggagaa
     1981 agaccggctg ctcacggagt tgtaccagca ggaacgccag cgagcagatc ggcaccgggg
     2041 gtacagcgac tttctcttac ggagcatgac gaccggtgtc attggcgtgg acttcgccgg
     2101 ccggctgacc agcatcaacc ccgccgcgct ggacattctg gacctctcga ccgaggacat
     2161 gggagaggag ctcgattctt tactcgaacg cgaccactcc atcatgatcc tccttgagag
     2221 tacgctggcg ggacaagcgg ttcagacgcg tcaggagctg cgcctacaga cgcgcgccgg
     2281 acgccagaaa tgggtcggcg ttcataccgc accgttgcac gaccacgacg ggatcctgat
     2341 cggcgcctca gccctgctga ccgacctgac cgcggtcaga aaactccagg acgagatccg
     2401 gagaaaggac cggttggcgg ccatcgggca gctgagcgcc ggcatcgcgc acgaaatccg
     2461 aaatcccctc gccgccatcg aagggttttc ggaactcctg gcgcgtcggg ttccggatga
     2521 aggcgcccgt gtcc
//
LOCUS       DBNGTT010000389         3773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1370718, whole genome shotgun sequence.
ACCESSION   DBNGTT010000389 DBNGTT010000000
VERSION     DBNGTT010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1370718"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /gene="plsX"
                     /locus_tag="AC16BC_08805"
     CDS             <1..978
                     /gene="plsX"
                     /locus_tag="AC16BC_08805"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR3241759.1"
                     /translation="GPAAVVEGALLAVADPRTRVILVGDEAKIRAELDRLRAPSGIRT
                     VHAPTVVGMDESASSVVRRRRDSSIWRATELVKSGEADAVVSAGHTGASMATAFFLLG
                     TLQGVERPAIATILPTLRGKAVLLDVGANVDSKPQHLVQFAIMGHVYAQRMLDVASPR
                     VGLLSIGEEDTKGNELTKETFKLLKQTQVNFIGNVEGRDVYTGTADVVVCDGFIGNVA
                     LKLSEGLAETIMQVLRREITDSVAGRLGFLLLRPAFRRFKRRVDYAEYGGAPLLGING
                     VSIICHGRSSPKAIKNAVLVARDFVAGRVNRHIRDDIERHLHQGSGTPG"
     gene            975..1964
                     /locus_tag="AC16BC_08810"
     CDS             975..1964
                     /locus_tag="AC16BC_08810"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR3241760.1"
                     /translation="MTSALRSRIIGTGSYVPEHIMTNADIERMVDTSDQWIVERTGIR
                     ERRIAAPSEATSDLAVAAATRALEAAGVRAPDLDLVLVATATPDMLFPATACLVQARL
                     GAKQAFAFDVSAACTGFLYGLSVADQYLRSGSARTVLLVGAEVLSRVMDWTDRTTCIL
                     FGDGAGAVVLRAERGERGVLSTHIHADGSMWDFIHVPGGGSRHPPSEDTLVRRLQFLR
                     MKGSETFKVAVRTLEETALEALKANGLTIADVRWLIPHQANRRIIEAVGARLGLPAER
                     VVVNLDRVGNTSAASIPIALDEVIRRGEVNPNDVLLFAAFGAGLTWGSAAVRW"
     gene            2052..3017
                     /gene="fabD"
                     /locus_tag="AC16BC_08815"
     CDS             2052..3017
                     /gene="fabD"
                     /locus_tag="AC16BC_08815"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3241761.1"
                     /translation="MTAAQHIAWLFPGQGSQTVGMGKLLHDQCPEARAVYVEASDALG
                     WDVARVSFDGPEAELNRTAVTQPALLTASVAAFRVLARKPIAASFAAGHSLGEYTALV
                     CAGAVPFAQAVRIVHHRGRYMQEAVPEGEGAMAAVLGLDQAGVAAACREASALGVVEP
                     ANLNSPGQIVIAGARAAVARAGELAAAAGAKRVVPLPVSVPSHCRLMADAARRLEVDL
                     RHATWSAPRVPVVTNVDAKPLTSTDAIVPALVRQLSSPLLWEDSIRFMLDQGVTTFVE
                     VGPGRVLSGLTKKIERSARALQADTPDTVEAVWAAVETLATEVAQ"
     gene            3014..>3773
                     /gene="fabG"
                     /locus_tag="AC16BC_08820"
     CDS             3014..>3773
                     /gene="fabG"
                     /locus_tag="AC16BC_08820"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR3241762.1"
                     /translation="MTDQQGQPAFGPAALADRVALVTGAAQGIGLSIAKQLAACGATV
                     VLADVDADKTQEAAKAVGGRALGIGMNVTRGAEVTAGVERVMAEFGRLDVLVNNAGIT
                     RDGLLIRMKEEDWDRVLDVNLKGTFHCTKAAMGVMVKQRRGRVISIASIVGVMGNAGQ
                     ANYAASKAAIIGFTKSIAREYANRGVTANAVAPGFIKTAMTDQLPEDVQTKLREQIPL
                     GRLGAPEDIAQAVAFLASDAAAYVTGQVLHVNGGM"
BASE COUNT          587 a         1290 c         1334 g          562 t
ORIGIN      
        1 ggcccggccg ccgtggtcga gggcgcgctc ctggccgtcg cggatccgcg gacgcgcgtg
       61 atcctggtcg gcgacgaagc caagatccgc gccgaactcg atcggctccg cgccccatcg
      121 ggcatccgaa cggtgcacgc gcccacggtc gtggggatgg atgaaagcgc ctcatccgtg
      181 gtccgccgcc gcagggattc ctcgatctgg cgcgccaccg aactggtcaa atccggagaa
      241 gccgacgccg tggtcagcgc gggccacacc ggcgcgtcga tggccaccgc gttcttcctc
      301 ctgggcacgt tgcagggcgt cgagcgcccg gcgatcgcca cgatcctccc caccctgcgc
      361 ggcaaggcgg tgctgctgga cgtgggcgcc aacgtggatt ccaagccgca acacctggtc
      421 cagttcgcga tcatggggca cgtctacgcg caacgcatgc tcgacgtggc gagcccccgg
      481 gtcggcctgt tgagcatcgg cgaggaagac accaagggca acgaacttac caaggaaacc
      541 ttcaagctgc tcaaacaaac gcaggtcaac ttcatcggca acgtggaagg ccgcgacgtc
      601 tacaccggca ccgcggacgt ggtggtctgc gacgggttca tcggcaacgt ggcgctcaag
      661 ttgagcgagg gactcgcgga aacgatcatg caggttctgc gtcgcgagat cacggactcg
      721 gtcgccggcc gtctgggctt tctgctgctg cgacccgcct ttcgccgatt caaacgccgc
      781 gtggactacg cggagtacgg cggcgcgccc ctgctcggga tcaacggcgt ctcgatcatc
      841 tgtcacgggc ggtcctcgcc caaggcgatc aagaacgccg tgctggtcgc gcgcgacttt
      901 gtcgcgggcc gcgtcaaccg ccacatccgc gacgacatcg aacggcacct gcaccaggga
      961 tcgggcacgc cgggatgacc tccgcgctgc gatcgcgcat catcggcacc ggctcgtacg
     1021 tccccgagca catcatgacc aacgcggaca tcgagcggat ggtggacacg tccgaccagt
     1081 ggatcgtgga gcgcaccggg atccgtgaac ggcggatcgc ggcgccctcc gaggcgacat
     1141 cggacctcgc cgtggcggcc gcgacccgcg cgctcgaagc cgcgggcgtg cgcgccccgg
     1201 acctcgacct cgtgttggtc gccaccgcaa ccccggacat gttgtttccg gccacggcct
     1261 gcctggtgca ggcccgcctg ggcgccaaac aggcgttcgc gttcgacgtg tcggccgcgt
     1321 gcacgggctt tctgtacggg ctgtcggtcg cggatcagta cctgcggtcc ggctccgcac
     1381 gcacggtgct gctcgtgggc gcggaggtcc tctcccgcgt gatggactgg accgatcgca
     1441 ccacgtgcat cctgttcggg gacggcgccg gcgccgtggt cctgcgcgcc gaacgcggcg
     1501 agcgcggcgt gctctccacg cacatccacg cggacggctc gatgtgggac ttcatccacg
     1561 tgccgggcgg cggctcgcgg cacccgccgt cggaagacac gctcgtgcgg cggctgcaat
     1621 ttctccgcat gaaaggcagc gaaaccttca aggtcgcggt gcgcacgctg gaagaaaccg
     1681 cgctggaggc cctgaaggcc aacgggctga ccatcgcgga cgtgcggtgg ttgattccgc
     1741 atcaagccaa ccggcgcatc atcgaggcgg ttggcgcgcg cctgggcctg cccgcggagc
     1801 gggtcgtggt caatctcgac cgggtcggca acacgtccgc ggcgtcgatc ccgatcgcac
     1861 tcgatgaagt catccgacgc ggcgaggtga acccgaacga cgtgctcctg ttcgcggcgt
     1921 tcggcgcggg cctcacgtgg ggctctgcgg ccgtgcgctg gtagcgcgcg cgacgcgatc
     1981 cgtcgcgcgc cccgcttgac aaccaggaac gatttcagca atatcaggcc accgttgacc
     2041 cggattttat cgtgaccgcc gctcaacaca tcgcatggct cttccccgga caaggctccc
     2101 agaccgtggg aatgggcaag ctgttgcacg accagtgccc cgaagcgcgc gccgtctatg
     2161 tcgaggcgtc cgacgccctg gggtgggacg tggcgcgggt gtcgttcgac ggcccggaag
     2221 cggagttgaa ccgcaccgcg gtgacacagc ccgccttgct cacggcgagc gtcgccgcat
     2281 tccgggtgct ggcccgaaaa ccgatcgccg cgtcctttgc cgcgggccac agcctgggtg
     2341 aatacaccgc cctggtgtgc gcgggcgccg tgccgtttgc gcaggcggtc cgcatcgttc
     2401 accatcgtgg gcggtacatg caggaggccg tgcccgaggg cgagggcgcc atggccgcgg
     2461 tgctcggcct ggatcaggcc ggggtggcgg ccgcgtgccg cgaggcctcc gcgctgggcg
     2521 tggtggagcc cgccaatttg aactcgccgg gtcagatcgt gattgccggg gcgcgcgccg
     2581 cagtggcgcg cgccggggaa ctggccgcgg ccgccggagc caagcgggtc gtgccgctcc
     2641 cggtgagcgt gccgtcgcac tgccgcctga tggccgacgc ggcgcgacgg ctggaagtcg
     2701 atctgcggca cgcgacgtgg tccgcgccca gggtgccggt ggtgaccaac gtggacgcca
     2761 agccgctgac ctcgaccgac gcgatcgtgc ccgcgctggt ccgacaactc tcctccccgc
     2821 tcctgtggga ggattcgatc cggttcatgc tcgatcaggg cgtcaccacg ttcgtggaag
     2881 tcggccccgg ccgcgtgctc tcgggtttga ccaagaagat cgagcgctcc gcccgcgcgt
     2941 tgcaggccga caccccggac acggtggagg cggtgtgggc cgcggtggag acgctcgcga
     3001 cggaggtggc gcaatgaccg atcagcaggg acagcccgcg ttcgggccgg ccgcgttggc
     3061 cgaccgggtg gcgctggtca cgggcgcggc ccagggcatc ggcttgtcga tcgccaaaca
     3121 actcgccgcg tgcggcgcga ccgtggtgct cgccgacgtg gacgcggaca agacccagga
     3181 agcggccaag gccgtgggcg gccgggcatt gggcatcggc atgaacgtga cccgcggcgc
     3241 ggaggtcacc gccggcgtgg agcgcgtgat ggccgagttc ggccggctcg acgtgctggt
     3301 caacaacgcc gggatcaccc gcgacggcct gctcatccgc atgaaggaag aggactggga
     3361 ccgcgtgctc gacgtgaacc tcaaaggcac gtttcactgc accaaggccg cgatgggggt
     3421 gatggtcaag caacggcggg gccgcgtcat cagcatcgcc tcgatcgtcg gggtgatggg
     3481 caacgcgggc caagccaact acgccgcgtc caaggccgcc attatcggct tcaccaagag
     3541 catcgcgcgc gagtacgcca accgcggcgt tacggccaac gccgtggcgc cggggttcat
     3601 caagaccgcc atgaccgatc agcttccgga agacgtccaa acgaagctgc gcgagcagat
     3661 cccgctgggt cggctcggcg cgcccgagga tatcgcccag gccgtggcgt tcctggcgtc
     3721 ggacgccgcg gcgtacgtca cggggcaggt gttgcacgtc aacggcggca tgt
//
LOCUS       DBNGTT010000390         3195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_476163, whole genome shotgun sequence.
ACCESSION   DBNGTT010000390 DBNGTT010000000
VERSION     DBNGTT010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_476163"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="AC16BC_08825"
     CDS             <1..1006
                     /locus_tag="AC16BC_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HIR3241763.1"
                     /translation="EQALMAAKAPRKPSTARRRKKPAGASSGLSAAALTEADAPQEVT
                     DLADAITRDNGRVLATYREPYNGRWVVFAALPIDQVEPTPYQRNISETHVRRLEAVIG
                     KLGRFLDPIIAVRADQGTEGATRYWTPNGNHRLTAMRTLGAKTITAIVIPEHAAAYQI
                     LALNTEKAHNLREKALEVVRMYEELVRLGEATEESYALEFEEPSFITLGLCYQERPRF
                     SGGAYHPLLKRADQFLTKPLRQAIDIRRSRAADLLALDDQVNQHVEALKARGLTSPYL
                     KAAVVARINPLRFRPKDAPALSYDDAMDRITKAAAKFNPAKLTSEDLARTGGAPEGAE
                     "
     gene            1051..1527
                     /locus_tag="AC16BC_08830"
     CDS             1051..1527
                     /locus_tag="AC16BC_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015591275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241764.1"
                     /translation="MAGGLSTGIHFGLTSGVITTLGLIVGLHAGTHSTLAVVGGVFTI
                     AVADALSDALGIHISKEAENTLSTRQIWLATLATFTAKFVIAGTFLAPLVLLPLATAI
                     TVSVVWGLLVLAVLSVRLARVQQLSPLPVILEHVGIGLVVVTLTHVIGDWVSRVFS"
     gene            complement(1556..2263)
                     /locus_tag="AC16BC_08835"
     CDS             complement(1556..2263)
                     /locus_tag="AC16BC_08835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3241765.1"
                     /translation="MEIPAGLRAAIFDFDGVIVDSEPVHFRWFQTLLAEEGVPLTRAD
                     YDAIYLGMDDRECFTEALTRHGKTAALPKVPALIERKSALLMAEVAKNPPVLPGAVEL
                     VRGLAKSVPLAICSGALRREIEAMLTHADILSCFAGIVSAEDVSHSKPHPEGYTKALA
                     LLNRTQPPARAIAASSCLVIEDSIAGIEAAKAAGMRCLAVANSYPEAALKQANAEAVV
                     ATLDGDVMAAVHQCFPS"
     gene            2384..3091
                     /locus_tag="AC16BC_08840"
     CDS             2384..3091
                     /locus_tag="AC16BC_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR3241766.1"
                     /translation="MNAKSRVVLSVSLFFNGLFLGMVLMGFKSPNVLTSFNADVDTPQ
                     VAKSAFVHEIGSVIGAVEIGERVYVAPTASVRGDEGQPIHIGNESNVQDSVVVHGLET
                     HEGKEVIAKNQVEVGGKKYSVYIGDRVSMAHQSQVHGPAKVGDDAFIGMQALVFKAEI
                     GGGTVVEPGAKVIGVKVGEKKIVPAGAILTKQADADALAAITDTYPFKDLNKAVVHVN
                     TQFADGYNGKKPAAHGH"
BASE COUNT          594 a         1067 c         1002 g          532 t
ORIGIN      
        1 tgaacaggct ctgatggccg ccaaggcgcc gcgcaaaccc tctaccgcgc gtcgccggaa
       61 gaagcccgcc ggcgcgtcat cggggctgtc ggccgcggcg ctgaccgaag ccgacgcgcc
      121 gcaagaagtc actgacctgg ccgatgccat cacccgcgac aacggccgcg tgttggccac
      181 gtaccgcgaa ccgtacaatg ggcgttgggt ggtgtttgcc gcgttgccca tcgaccaggt
      241 ggagcccacc ccgtatcagc ggaacatctc ggaaacgcac gtgcgccggc tcgaagccgt
      301 gatcggaaaa ctcggccgct tcctggaccc catcatcgcg gttcgcgcgg accagggcac
      361 tgaaggcgcc acacggtact ggacacccaa cggcaatcac cggctcaccg ccatgcgcac
      421 gctgggcgcc aagaccatca ccgcgatcgt gattccggaa cacgccgcgg cctaccagat
      481 cctcgcgctc aataccgaaa aggcgcacaa cctgcgggaa aaagcgctgg aagtggttcg
      541 catgtacgag gaattggtac ggttgggcga ggcgaccgag gagtcctacg cgctggagtt
      601 cgaggagccg tctttcatca cgctgggact gtgttatcag gagaggccgc gcttttcagg
      661 cggcgcgtat catcccctgc tcaaacgcgc ggaccaattc ctgaccaagc cgctccggca
      721 ggcaatcgac atccgccgct cgcgcgccgc cgacctattg gcgctggacg atcaagtgaa
      781 ccagcacgtc gaggcgctca aggcccgagg tctgaccagc ccgtatctca aagccgcggt
      841 ggtcgcgcgc atcaaccccc tgcgctttcg ccccaaagac gcgcccgcct tgagctacga
      901 cgacgccatg gaccgcatca ccaaagccgc ggccaaattc aaccccgcga aactcacgtc
      961 ggaagacctc gcgcgcaccg gcggggcgcc ggagggcgcc gagtgaacgc catccgatga
     1021 cgctgaatcc agcgcccttg acccgttaac atggccggcg gactcagcac cggcatccac
     1081 ttcggcctga cctccggcgt gatcacgacg ttggggctga tcgtcgggct ccacgccggg
     1141 acccattcga cattggccgt ggtcggcggc gtgttcacga tcgcggtggc ggatgcgctc
     1201 tcggacgcac tgggcatcca catttcaaag gaagcggaaa acacgctgtc gacacgacag
     1261 atctggctgg cgaccttggc gacgtttacg gccaagttcg tgatcgcggg aaccttcctc
     1321 gcgccgctcg tgttgctgcc gctcgcaacc gccattacgg tgagcgtggt ctgggggtta
     1381 ctcgtgctcg cagtgctgag cgtgcggctc gcgcgggttc agcaactctc gccgctcccg
     1441 gtgatcctgg aacacgtggg catcggctta gtcgtggtga cgctgacgca cgtgatcggc
     1501 gactgggttt cccgagtgtt ctcttaagca gccaggcaac tgttcgacgc agtcgtcagc
     1561 tgggaaaaca ctggtgcacc gcggccatga cgtccccgtc cagcgtcgcg accacggctt
     1621 cggcattcgc ctgcttcaac gcggcttcag ggtaggaatt ggccacggcc aggcaccgca
     1681 tgcccgcggc cttggcggcc tcgatgcccg cgatggaatc ttcgatcacc aggcacgacg
     1741 aggcggcgat ggcgcgcgcc gggggttggg tgcgattgag cagggccagg gccttggtgt
     1801 agccctcggg gtgcggtttg ctgtggctca cgtcttccgc gctgacgatg cccgcgaaac
     1861 acgagagaat gtccgcgtgc gtgagcatgg cctcgatctc ccggcgcaac gcgcccgagc
     1921 agatcgccag cggcacgctc ttggccaggc cccgcacgag ttccaccgcg cccggcagca
     1981 ccggcggatt cttcgccacc tcggccatca ataacgcgga cttgcgttcg atcaacgccg
     2041 ggaccttggg gagcgcggcg gttttcccgt gccgggtcaa cgcttcggtg aaacactcgc
     2101 ggtcgtccat accgaggtag atcgcgtcgt agtccgcgcg cgtgagcggt acgccttcct
     2161 cggccagcaa cgtctggaac cagcgaaagt gcaccggctc gctgtccacg atcaccccgt
     2221 caaaatcgaa aatcgccgcc cgcagtcccg ccggaatctc cacgcttgac ccccaaaaac
     2281 gatttgtgct acgttcgctc cccgtccgcg gtcccctccc gactcgccgg gttttcgcgg
     2341 acgcccatcg taccacagcg acatgttacc tgaaaggagt tgtatgaacg cgaagagccg
     2401 tgttgtgttg tcggtcagct tgttcttcaa cggtctgttc ctcgggatgg tcctgatggg
     2461 attcaagagc cccaatgtcc tgacgtcctt caacgcggac gtggacaccc cgcaggtggc
     2521 caaatccgcc ttcgtgcacg agatcgggtc ggtcatcggc gcggtcgaaa tcggcgagcg
     2581 cgtctacgtc gcccccaccg cgtccgtgcg cggggacgag ggtcagccga tccatatcgg
     2641 caacgagagc aacgtgcagg acagcgtggt ggtgcacggc ctcgagaccc atgaaggcaa
     2701 agaagtcatc gccaagaatc aggtggaggt cggcggcaag aaatactccg tctacatcgg
     2761 ggatcgcgtg tccatggccc accagtcgca ggttcacggc ccggccaagg tcggagacga
     2821 cgcgttcatc ggcatgcagg ccctggtgtt caaagccgag atcggcggcg gcaccgtggt
     2881 cgagcccggc gccaaagtca tcggcgtcaa ggtcggcgag aagaagatcg tgccggccgg
     2941 cgcgattctg accaaacagg ccgacgccga cgcgctggcc gccattacgg atacctatcc
     3001 tttcaaggat ctgaacaagg ccgtggtgca cgtgaacacg cagttcgcgg acggatacaa
     3061 cggcaagaaa cccgccgcgc acgggcactg atcgttcgac tcgggagggg cgcgcgcgta
     3121 cgcccctccc ccaccatgcg aggtctgcca tgaaacgcgt cctcgttcca ctcgctccgg
     3181 gcttcgaaga aatcg
//
LOCUS       DBNGTT010000391         2973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1243072, whole genome shotgun sequence.
ACCESSION   DBNGTT010000391 DBNGTT010000000
VERSION     DBNGTT010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1243072"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="AC16BC_08845"
     CDS             complement(<1..459)
                     /locus_tag="AC16BC_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241767.1"
                     /translation="MTTPAASRHARVATAFERTIARLEALRRRGTAASHRFTRWRIGI
                     FLAGAAAVTAAAFADPEHGGWIPLAIFGAAFFAVAYRHGRLKTTLRRLDAWIALKRTH
                     LARVRLDWSAIPAADHPAPEHHPYAVDLDVTGAHSLLRWLDTTVSTNGRGE"
     gene            complement(456..1298)
                     /locus_tag="AC16BC_08850"
     CDS             complement(456..1298)
                     /locus_tag="AC16BC_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3241768.1"
                     /translation="MSTPKIRVLILGFGRLGDALVRRHASRYEFRGIKRTPVEAAPCP
                     VFLMPLDDDRINDHLAWADHVLFCPAAPASDTDAYRATYLDNMVTLTRRLIEHRITPR
                     SIILISSTGVYPESADEPIDEDYQPPVESERQDVLLQTEHALIQSGLPYVILRCAGLY
                     GAGRDAFRERLADGRINSTMLTTQYIHFIHRHDVCDAIDLAIRRHLTAAIFNLVDDSM
                     IRRADFYRFLSTLYGLPIPDRGPAPETGRDRLISNARVKSGLGLTLSSPRITDYLAAT
                     AALR"
     gene            complement(1352..>2973)
                     /locus_tag="AC16BC_08855"
     CDS             complement(1352..>2973)
                     /locus_tag="AC16BC_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3241769.1"
                     /translation="VVQSGGPGGQTSLFLRGGNSNHVLVLIDGVKVNSPTTGAFDISH
                     LTVDQIERIEVLRGAQSPLYGSEAVSGVINIITKRAGKSVQNAVTIEGGSYHTRSGAL
                     THSGRGPVWDRTLSVASRWVVRGFSRADEDLGNTEPDGYANTAFSMNLGRSAGAGGRA
                     DINLRLTDATADIDDAYPAPTFQLSDSPAKSRNKAAVAGLTIAGPLSARWDHRLVIGW
                     ARDHGTTTQGSFGDTDIDAQSRQVDWAHTMGVGADNLLTVGYEYQGRLADIPGAGEHH
                     LATNAVYVQDQLAMFDPLFITIGGRTDDSNRFGRHNTYKAGASLKLSSWRSRLFGNYA
                     TGFRGPTLNDLYYPGFSNPNLRPEASAGYETGISVEVVPGLLTVQATRFRTNYDDLIA
                     LDNLFVPQNIATAETSGFELSGTWTVSPQSTITGNYTVTNAINGSTGDRLLRRPHNKG
                     NLVWTFHPDPATDFRIDYRCIGSRRDTPDGFTMLTLPHYALVNLAASERVSPETAVFM
                     RLDNALNRNYEEISGYGTPGRSVYAGVTVQF"
BASE COUNT          505 a          892 c         1057 g          519 t
ORIGIN      
        1 ctcaccccga ccgttggtgg agaccgtggt gtcgagccag cgcagcaggg aatgcgcgcc
       61 ggtgacgtcc agatccacgg cgtacggatg gtgctcgggc gcgggatgat ccgcggccgg
      121 gatggccgac cagtcgaggc gaacgcgagc cagatgggtg cgttttaacg cgatccaggc
      181 gtcgagccgc cgcaacgtgg tcttgagcct gccgtgccga tacgccacgg cgaagaacgc
      241 ggcgccgaaa atcgcgagcg gaatccatcc gccgtgttcg ggatcggcga acgcggccgc
      301 ggtcacggcg gccgcgcccg cgaggaaaat cccgatgcgc cagcgcgtaa agcggtggct
      361 cgccgccgtg ccccggcgcc gcaacgcctc gaggcgcgcg atggtccgtt caaaggccgt
      421 cgcgactcgg gcgtgacgag aggcggcggg agtcgtcatc gaagggcggc ggttgcggcg
      481 aggtagtcgg tgatacgggg cgaagagagc gtcagcccca acccgctctt gactctggca
      541 ttggaaatga ggcgatcgcg gcccgtctcc ggggccggac cgcggtcggg aatcggcagg
      601 ccgtagagcg tggacaggaa ccggtagaag tcggcgcgac ggatcatgga gtcatccacc
      661 aggttaaaga tagcagcggt gaggtgacgg cggatcgcca ggtcgatggc gtcgcacacg
      721 tcgtgtcggt ggatgaagtg tatatattgt gtcgtgagca tcgtggagtt gatgcggccg
      781 tcggccagcc gttcccggaa cgcgtcgcgt cccgcgccgt acaggccggc gcagcggagt
      841 atgacatacg gcagcccgct ctggatcaag gcgtgttcgg tctgcaggag gacgtcttgt
      901 cgctcggatt cgaccggcgg ttgatagtcc tcgtcgatgg gctcgtccgc agactccgga
      961 tacacgccgg ttgaactgat caagatgatc gagcgcggcg tgatccggtg ttcgatcaga
     1021 cgccgcgtca gcgtcaccat attgtccagg tacgtggcgc ggtaggcgtc cgtatccgac
     1081 gcgggcgcgg ccgggcaaaa cagcacgtga tcggcccacg cgagatgatc gttgatccgg
     1141 tcgtcgtcga ggggcatcaa gaacaccgga cacggggcgg cctcgaccgg cgtgcgcttg
     1201 atgccgcgaa actcgtaccg tgatgcgtga cggcgaacca aggcgtcacc gagtctgccg
     1261 aaaccgagga tcagtacccg aatcttgggc gtggacacgg ggtggagcct accacaacgc
     1321 cgcgtggact caaaaccgga cgcgtccgcg gttaaaactg tacggtcact cccgcataga
     1381 cggatcgtcc cggagtgccg tagccggaga tctcttcgta gttccggttg agggcgttgt
     1441 cgagtcgcat gaataccgcg gtctccggcg acacccgctc cgacgccgcg aggttcacca
     1501 gcgcgtagtg cggaagcgtc agcatggtga acccgtcggg ggtgtcccgt cggctgccga
     1561 tacagcggta gtcgatccgg aaatccgtgg ccggatcggg atgaaacgtc cagaccaggt
     1621 tgcccttgtt gtgcggtcgt cgcaggagtc gatccccggt cgagccgttg atggcgttgg
     1681 tcacggtgta gttgccggtg atcgtggact ggggagacac ggtccacgtc ccggacagtt
     1741 cgaagcccga cgtctcggcg gtcgcgatgt tctgggggac gaacagattg tcgagcgcga
     1801 tcagatcgtc gtagttcgtg cgaaagcggg tggcctggac cgtgagcagt ccgggaacca
     1861 cctcgacact gattcccgtc tcgtagccgg cggacgcttc cggacgcagg ttggggtttg
     1921 agaatccggg gtagtacagg tcgttcaacg tcggtccgcg gaatccggtc gcgtagttgc
     1981 cgaacaggcg agaccgccaa gacgacaact tgagcgacgc gccggccttg taggtgttgt
     2041 gacgaccgaa ccggttgctg tcgtcggtcc ggccgccgat cgtgatgaac aacgggtcga
     2101 acatggcgag ctggtcctgg acgtagaccg cgttggtggc caggtggtgc tcgccggctc
     2161 ccgggatatc ggccaggcgg ccctgatatt cgtaccccac ggtcaacaag ttgtcggccc
     2221 cgacgcccat ggtgtgggcc caatccacct gccgggattg cgcgtcgata tcggtgtcgc
     2281 cgaacgagcc ctgcgtggtg gttccgtggt cgcgggccca gccgatgacc aggcggtggt
     2341 cccaccgcgc gctgagcggt ccggcgatgg tcagtccggc gaccgccgcc ttgtttcggc
     2401 tcttggccgg gctgtccgag agttggaacg tcggcgcggg gtaggcgtcg tcgatgtccg
     2461 cggtcgcgtc ggtaagccgc agattgatgt ccgcgcgccc gcccgcgccg gcgctccggc
     2521 ccaggttcat cgaaaaggcg gtgttggcgt agccgtctgg ctcggtgttg cccaggtcct
     2581 cgtccgcgcg ggaaaaaccg cgaacgaccc atcgcgacgc cacggacaag gtccgatccc
     2641 acaccgggcc gcggcccgaa tgggtcaatg ctccggatcg cgtgtggtaa gagccgcctt
     2701 cgatcgtgac ggcgttctga acggatttcc cggcccgttt ggtgatgatg ttgatcacgc
     2761 cggacacggc ttcggagccg tacaacggac tttgcgcgcc tcggagcacc tcaatccgtt
     2821 cgatctggtc caccgtgagg tgcgatatgt cgaacgcgcc ggtggtgggg gagttgacct
     2881 tcacgccgtc gatcaacacc agcacatggt tggagttgcc gcctcgcagg aagagcgagg
     2941 tttggccacc gggtcctccg ctctgcacca cgt
//
LOCUS       DBNGTT010000392         5642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1498657, whole genome shotgun sequence.
ACCESSION   DBNGTT010000392 DBNGTT010000000
VERSION     DBNGTT010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1498657"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1401)
                     /locus_tag="AC16BC_08860"
     CDS             complement(106..1401)
                     /locus_tag="AC16BC_08860"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR3241770.1"
                     /translation="MPGVVILGTQWGDEGKGKIVDLLSPEADVIVRFQGGHNAGHTVV
                     VDTDQFVLHLVPSGILHPGKICVIGNGVVIDPAALLEEVDGLIGRGVDLKERLFVSDR
                     AHLIMPYHKAIDKESEKHKGARKIGTTGRGIGPAYADKMARVGLRVSDLLDADLFRQK
                     LTRSLTDMNYLLGRLYGVKGFELDAVYEAYLGYAARLRSFIVDTSELLHDAITSGRRV
                     LFEGAQATLLDVDHGTYPFVTSSSATVGGALIGTGVGPRGIDRVLGVAKAYSTRVGEG
                     PFPTELHDDRGGAELRARGQEFGATTGRPRRCGWFDAVVVRYAVRLNGLSGLAITKLD
                     VLDDCGDLKVCTGYRLDGRTLTTVPAVPEHYERCEPIYETLPGWKEPTVGITRYDDLP
                     MNAKQYLERLSELVNCPLDIISTGSKRDQTIIRCRAFTA"
     gene            complement(1615..2241)
                     /locus_tag="AC16BC_08865"
     CDS             complement(1615..2241)
                     /locus_tag="AC16BC_08865"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3241771.1"
                     /translation="MVRVYRTVVLAAVVFASAHVAEAGTVSGKARVAGKDSAANIVVY
                     LEGVNGTFKLPEKRPEMNHINLQFQPSVMAVLKGTTVDFPNSDSVFHSAFSISPSNPF
                     DLGLYQKGREKFVVFKNPGVVELFCHIHSHMHGFILVLDNPYFATTNPDGGFSIANVP
                     DGDYTVKAWASPTATMTKTVSLNGDKTVNMDFTLTADSDSAFAATASR"
     gene            complement(2283..3392)
                     /locus_tag="AC16BC_08870"
     CDS             complement(2283..3392)
                     /locus_tag="AC16BC_08870"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3241772.1"
                     /translation="MKVRTMGRWLVAAAVIVASVVSMSSVILAGSTEGDAKVQKARRT
                     LITLRTLDQMTALPGELGPLPAVPVPASNPQTPAKIELGKMLFFDPRLSANDHWACAT
                     CHNPSFGYSDGLPRSLGFGDEKELGRHSPTVINIAYNSAQFWDGRAATMEDQATGPIV
                     AAREMNSDPQQMINEVSDIPYYREKFKEVFGGPPTMKTVGMAIASFERTIVTGESRFD
                     RYAKGDKKALTDQEKRGLTMFISKAACTQCHNGPNFTDNSFQNLGVPQEGPLAEDLGR
                     YGVTKDAKDKGAFKVPSLRNIAMTPPYMHTGVFKTLEEVMDFYNKGGGPSPNKSPKIL
                     PLNMTDSEKKDLVAFLKTLTGDLPQIVPPQLPPRS"
     gene            complement(3529..4140)
                     /gene="yihA"
                     /locus_tag="AC16BC_08875"
     CDS             complement(3529..4140)
                     /gene="yihA"
                     /locus_tag="AC16BC_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR3241773.1"
                     /translation="MPIHEAHFVRACAHPGEFPKSGWPEVAFVGRSNVGKSSLLNALI
                     GRKQLAKVSATPGKTQTINFFAVDDAYYFVDLPGYGYAKVPQPVQRAWRALIEAYLAR
                     RATLCAVVLLVDARHPPTDLDLTMHQWLAAYEVPEVVVATKADKVPRGQRRAAEARIV
                     SALGLPEGHVPLFVSAHTGEGRPALQALIKDAVAPGRRRPARP"
     gene            4298..5026
                     /locus_tag="AC16BC_08880"
     CDS             4298..5026
                     /locus_tag="AC16BC_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241774.1"
                     /translation="MAIEFSVTYEKASRLFDAGRYPEAEDLYLKLVEAGGSGYADIYN
                     RLGLITAFQGQIETAVEWFLKAVQANPRYTEACLNLAVIYADLSRYDEADQVYHEAAR
                     LVTDAPRSTDFYVRGRLANEHAALGDRYVNLGLPDEALDEYRKALGLCPMFPDIVTRV
                     GMVLREQGAYDEAIRVFLRAKELSPDYVPATVHLGITYYMSGFPDLAREEWESIPAEA
                     ITGEHTAVYLALAKKDCLDPEVIS"
     gene            5074..5642
                     /locus_tag="AC16BC_08885"
                     /pseudo
     CDS             5074..5642
                     /locus_tag="AC16BC_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007727737.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         1075 a         1837 c         1657 g         1073 t
ORIGIN      
        1 ccttccaacc gtgcttgcgc cgtcaggcgc cgatcaatgc gccggaggct tcatctcaca
       61 tggcgaagca aggcggcgga tcgtatcaga cgcgcgttcg cagtgtcaag ccgtaaacgc
      121 gcgacaccga atgatggttt ggtcgcgttt cgatcccgtg gaaatgatat ccagcggaca
      181 gttcacgagt tcggagagcc gctccaggta ctgtttcgcg ttcatcggga gatcgtcata
      241 ccgggtgatc cccaccgtcg gttccttcca gcccggcagc gtttcgtaga tcggctcaca
      301 tcgttcgtag tgctccggca ccgcggggac ggtggtcagc gttcgaccgt cgagacgata
      361 tcccgtgcac accttgaggt cgccgcagtc atccaagaca tccaacttgg tgatcgctag
      421 tcccgaaagg ccgttcaatc gaacggcgta ccgcaccacc acggcgtcga accatccgca
      481 ccggcgaggt cgaccagtgg tcgcgccgaa ttcctggccg cgagcccgca gctccgctcc
      541 gcctcgatca tcgtggagtt cggtgggaaa cggtccctcg cccacgcgcg tcgaataggc
      601 tttcgccacg ccgagcaccc gatcgatccc gcggggcccc acgccggttc caatgagcgc
      661 cccgcccacc gtggcgctcg atgacgtgac gaacggatac gtgccatgat cgacgtcgag
      721 cagcgtggcc tgtgcgcctt cgaacaacac ccgccggccc gacgtgatcg cgtcgtggag
      781 caactccgag gtatccacga taaacgaacg cagccgcgcc gcgtacccga gatacgcctc
      841 gtacaccgcg tcgagttcga accctttgac gccgtacaaa cgtcccaaga ggtagttcat
      901 atccgtcagg ctgcgcgtca acttctggcg gaacagatcc gcgtcaagga gatccgagac
      961 gcgcaacccg acccgcgcca tcttgtcggc ataagccggg ccgatgccgc gtcccgtggt
     1021 gccgatcttc cgggcaccct tgtgcttctc gctctctttg tcgatcgcct tgtggtacgg
     1081 catgatcaag tgggcgcggt cgctgacgaa caaccgttcc ttcaggtcca ctccccgccc
     1141 gatcaggccg tcgacttcct ccaacagcgc cgccggatca atgaccacgc cgtttccaat
     1201 gacgcaaatc ttgccgggat ggaggattcc cgacggaacg aggtgaagga cgaattgatc
     1261 ggtatccacc accacggtat gtccggcatt atgcccgccc tgaaacctga cgatcacgtc
     1321 ggcttccggc gagaggagat ccacgatctt cccctttccc tcgtctcccc attgggtccc
     1381 cagtatcacg acgccaggca taccatcctc acggcgagcg gcgaaaataa acgaagccct
     1441 gaacgcccgg ttcagggctt cgcgtaacaa cggcggtatg ttgccttgcg ccaacctcca
     1501 aagtcaaggc gaccatgaag ccggaacaga cggagaattg ggacgtcccc gaaaaagcga
     1561 acggcgtaga tcggggcccg atctacgccg ttccattgcc aatcacgcgg tgcgtcagcg
     1621 cgacgccgtc gccgcgaacg ccgaatcgga gtcagccgtc aacgtaaaat ccatgttgac
     1681 ggtcttgtcc ccgttcaggc tcaccgtctt ggtcatcgtc gccgtggggc tcgcccacgc
     1741 cttcacggtg tagtccccgt ccgggacgtt cgcgatcgag aatccgccat caggattggt
     1801 ggtcgcgaaa tacgggttat ccaagaccag gatgaacccg tgcatgtggc tatggatgtg
     1861 gcagaacaac tcgaccaccc cggggttctt gaacacgacg aatttttccc gccctttctg
     1921 atacagcccg agatcaaacg ggttggatgg cgagatcgag aaggcgctgt ggaacacgga
     1981 atcgctgttc ggaaaatcaa ccgtcgttcc cttcaacacc gccatcacgc tcggctggaa
     2041 ctggaggttg atgtggttca tttcgggccg cttctccgga agcttgaagg tcccgttgac
     2101 cccttccaag tacaccacga tgttggccgc gctgtccttc cccgccacgc gcgccttccc
     2161 actcaccgtg cccgcctcgg ccacgtgagc cgacgcgaag acgaccgccg ctagaaccac
     2221 cgttcgatac acgcgtacca tacgtggcct ccttgtcagt gttgtccgat tacgttgtcc
     2281 tgtcacgacc gcggcggaag ctgcggcggc acgatctgcg gcagatcgcc cgtcagcgtc
     2341 ttgaggaacg ccaccaggtc tttcttctcc gagtcggtca tgttcaaggg aagaatcttc
     2401 gggctcttgt tcgggctcgg cccgcccccc ttgttgtaga agtccatgac ttcctccaag
     2461 gtcttaaaca cgccggtgtg catgtacggc ggggtcatgg cgatgttgcg caacgacggg
     2521 actttgaacg cgcctttgtc cttggcatct ttcgtgacgc cataccgccc caaatcctcg
     2581 gccagcggtc cttcctgcgg gacgccgaga ttctggaaac tgttgtcggt aaagttcggg
     2641 ccgttgtgac actgggtgca ggcagccttg ctgatgaaca tcgtcagtcc gcgcttttcc
     2701 tggtcggtga gcgccttctt atcgcccttg gcgtatcgat caaaccgaga ttcgcccgtg
     2761 acgatggtgc gctcgaacga cgcgatggcc atcccgaccg tcttcatggt gggcggcccg
     2821 ccgaagacct ctttgaattt ctcgcgataa tagggaatgt ccgaaacctc gttgatcatc
     2881 tgttgcgggt cagagttcat ctcccgagcg gcgacgattg ggccggtcgc ctggtcctcc
     2941 atggtcgccg cgcgtccatc ccagaactgg gccgagttgt aggcaatgtt gatgacggtc
     3001 ggtgaatgcc gccccagttc cttttcgtcc ccgaacccca gcgagcgggg cagcccatcg
     3061 ctgtacccaa agctcgggtt gtgacaagta gcgcacgccc agtggtcgtt cgccgacaac
     3121 cgcggatcga agaagagcat tttgcccagt tcgatcttcg ccggagtctg aggattgctg
     3181 gccgggacag gaaccgccgg cagcggcccg agttcgcccg ggagcgcggt catctgatca
     3241 agcgtcctca atgtgatcaa cgttcggcgg gccttctgaa ccttggcatc accctccgtt
     3301 gatccggcca gaatgaccga ggacatggac accacggacg cgacgatgac cgcggccgct
     3361 acgagccatc ttcccatcgt tcgcaccttc ataccccctc ctccatgaat gacggaccac
     3421 tcgccgaccc aacgggcgtt aaagatatca gccatcgggt gactgtcaag caaaccgcga
     3481 cgtggaccgt gaggaccccg aaccggggac ggagagcgag ggggggtatt aaggacgagc
     3541 gggacggcgt cgtccgggag cgaccgcgtc cttgatgagc gcttgcaatg ccggacgtcc
     3601 ttctcccgtg tgcgcggaga cgaacaacgg gacatgacct tcggggagac caagcgcgga
     3661 gacgatgcgc gcctccgccg cgcggcgctg tcccctcggg accttgtccg ccttggtggc
     3721 gaccacgact tccgggacct cgtacgccgc gagccattga tgcatggtca gatccaggtc
     3781 ggtcggcgga tgccgggcgt ccacgagcaa gaccaccgcg cacagcgtcg cccgacgagc
     3841 caggtaggcc tcgataagcg cgcgccacgc ccgttggacc ggttgcggca ctttggcgta
     3901 tccgtaaccc gggaggtcca cgaagtagta cgcatcatcg accgcgaaaa aattgatcgt
     3961 ctgcgtcttc ccgggcgtgg cgctcacctt ggccaactgc ttgcggccga tcagcgcgtt
     4021 gagcaaggat gactttccga cgttcgatcg accgacaaac gcgacctccg gccatcccga
     4081 cttcggaaac tctccgggat gggcgcacgc ccggacaaaa tgcgcttcat ggatcggcat
     4141 gcgaaacggg aactcccaca ctcgttcgac aatacatgag cagcgcactc tagcgaagcg
     4201 cctcgggtgg tgtcaatcaa tgccggtacg gcgtctcccg ccgattgaca taattttttc
     4261 gtttgttgta cagtttcgcc gcgcgtggga gtcggccgtg gcgatcgaat tttccgtgac
     4321 gtatgagaag gcatcaagat tgttcgacgc gggccgatac cctgaagccg aagacctcta
     4381 tctgaaatta gtcgaagccg gcggcagcgg ctacgcggat atctacaatc gtctcggact
     4441 catcaccgcg tttcagggac agatcgaaac tgcggtggag tggttcctca aagccgtcca
     4501 ggccaacccg cgctacaccg aagcgtgcct gaatctcgcg gtcatctacg cggatcttag
     4561 ccggtacgac gaggccgacc aggtctatca cgaagccgcc cggttggtca ccgacgcccc
     4621 gcggtcgacc gacttctacg ttcgaggacg attggccaac gaacacgcgg cgctcggcga
     4681 tcgctacgtc aatctcgggc tgcccgacga ggcgttggac gaataccgca aggcactcgg
     4741 cctgtgtccg atgtttcccg acatcgtcac cagggtgggc atggtcctgc gggagcaggg
     4801 cgcctacgac gaagcgattc gcgtgttcct ccgggccaag gaactctccc cggactacgt
     4861 cccggccacc gtccatctcg ggatcacgta ctatatgagc ggatttcccg atctggcccg
     4921 tgaagaatgg gagtcgattc ccgccgaggc catcaccggg gaacacaccg cggtgtatct
     4981 ggccttggcc aagaaggact gtttggaccc cgaggtgatt tcctagccga cgtgcgcggc
     5041 ctccccgccc acgcgggtgt tcccaccgct ccggtgtccc atcccctgct tcgcatcagc
     5101 gatctgaccg tcgagtttcc gtcgacgcgc ggtcgcgcgc gcgcggtcga tcacgtcagc
     5161 ctcgacgtct cgcgaggaga agtcctcggc gtcgtgggcg aatcgggttg cggcaaaagc
     5221 atgaccgcgc tcgccgtgct gggcctcgtg ccggctccgg gacgaatcgc cggcggacgc
     5281 gtgctgttcg acggacaaga cctgctcacg atacccccga tcgcgctcca gcagatccgc
     5341 ggcaaacgga tcggcatgat ctttcaggaa cccatgacgg cgttgaatcc ggtcctgacg
     5401 gtcggggagc agatcgcgga atcgatccga catcacctca aattctcacg cgcagacgcc
     5461 aaaacccgca ctatcgaatt gctccgcctg gtcgggatcc cctcggcgga atcccgatgg
     5521 gacgagtacc cccaccaact cagcggctga atgcgccaac gtgtgatgat cgccatggcg
     5581 atcgcctgtg atcccgacgt gatcttcgcg gacgaaccga ccaccgcgct cgacgtcacg
     5641 gt
//
LOCUS       DBNGTT010000393         7331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2520506, whole genome shotgun sequence.
ACCESSION   DBNGTT010000393 DBNGTT010000000
VERSION     DBNGTT010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2520506"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="AC16BC_08890"
     CDS             <1..249
                     /locus_tag="AC16BC_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241775.1"
                     /translation="GVTLISREDEKLVRSRLHEIAEIAKEFLIRTVFLLQQSEPIPAW
                     SGRRTSSISMADSRATLVVTDERILPTKDWKIKPEWCD"
     gene            450..2168
                     /locus_tag="AC16BC_08895"
     CDS             450..2168
                     /locus_tag="AC16BC_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3241776.1"
                     /translation="MVHEPLKPAPAGAQPAPENLLVESAREVMRALVKAVKASKMYLP
                     EHEICRKFREDFAARLSGHLEEHGDLALTVKGGEFWVGSTPVYEEANRHENLAFRCSS
                     DGLRELTIHRGLTREDLDRLIEILTSDARPLDNDIVTRLWEQPIPHVTYEIAEVDEAE
                     GGEVELSSRPPSEGASSEAADDRDPGTEAPAPEPEVVPESLRHDSPVFALTDEEIAAL
                     QRQVVEDTTQDYVGQLIDILESILALDDDEQSFLEVLQVLDDIVATSVRQGRFERAND
                     VVARLLALRDDEARVSARSRELIRVVWTQMGTWERVAPLEDPLNRQGAWEADALSEYL
                     GQLPSSAGDAFITLLDHVQTAKGRRILCDALTAVGGGAVETILTRLPDAPWYVARNLI
                     YVLGLVKDVRAQPALEAVMTHPDARLRKEALRALGDMGSDRLAAAIPRWIGDPDEGVR
                     LQVLRLARRHPPQGFLAMLTALINDRGFSRRQDAEQREVFETLADVGGDAALPLIRSW
                     LETGRGWPRFWSARHDPRARHAVAALRRIGSPAAIALLRETVTSGRGVILEDARRALH
                     DLERAA"
     gene            2165..3637
                     /locus_tag="AC16BC_08900"
     CDS             2165..3637
                     /locus_tag="AC16BC_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3241777.1"
                     /translation="MTPRGSAPRVATPKLFEAVKGSHIRDENLHRIGKRLVYQVHILL
                     KTAHIHLTGNVALSQAADRLVKTVGEVGGWRAEPVLRQDGDHLYLDDIRLKADLEGYI
                     GFSELIAAMQARGVGSLRFGGGLGTDEVMAFAHEWATAATTTAEELGAALHRVGVSHV
                     EAAPPPSARAKPAREHESQKAVAKRTYFRTISAVAEVMESVKLKQAVGVRKSKRVVHN
                     LVDVLLQDESTLLGLTTLRSHDEYTYSHCVNVCILALSIGQRLGYERSHLSELGVAAL
                     FHDVGKADIPLEILNKAGEFTPEDWRVIRRHPVFGVKSLLRLKGLDDVSTKIVIAGFE
                     HHLNYDLSGYPKIAMPRKLTLFGRIISLADCYDAMTASRVYSRVPLAPDKALSLMAAK
                     AGTVYDPLLLKVFANTMGVYPVGTVVRFDTGELAVVVGSHPAPERRAHPVVKLIRDAS
                     GRDVDGETIDLADPDARPSRRIVETADARSLGIDLARYFL"
     gene            3772..4062
                     /locus_tag="AC16BC_08905"
     CDS             3772..4062
                     /locus_tag="AC16BC_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241778.1"
                     /translation="MKKLVVLPLIAALMILPLTGCESKKLKAENASLKQQVDSLTQEK
                     NGLAAKADEMTKANADLQAKIGDLEKKVEELSKPKPAPKPAAKAPAKKTTKK"
     gene            4213..5703
                     /locus_tag="AC16BC_08910"
     CDS             4213..5703
                     /locus_tag="AC16BC_08910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3241779.1"
                     /translation="MQVSGSLSPRAPFRALQRLHLTTKLLLVMGVLTVLSLGSVFLFY
                     AYNERLLLREITDNINDLSSAIQISVEELTSQESTNEGRLKDYVNRLAKKGVREISII
                     SNAEKVIASSNPSRVGQRVNPSRKDLFITAKLGEEGAPGAQVHNLLVPIVVGKQQVGF
                     AHIMLVMDDYENLLRTNLVRRLMVTGGIFGLGIGLALFLSWRYTRPIYQVVDVAKQVA
                     AGNLSATLPQERGDEIGELIASVNEMVDRLREQRVLEDRLREAERFSALGQLAAGIAH
                     EIRNPLNFISLSLDRVRDVIKRDDGARSAEVPALLGSIKGEVFRLNRLIDGFLSYGRP
                     VRLTRRWVSVRGLLDDVIALVRQKAEEAKVDLSVEGAEPSPCFLDPEQITTCLMNVML
                     NAFQSMPDGGRLRVVLDLEADGNRAAERFVRLSVEDTGAGIPPEDLARVFEPYFTTKK
                     LGIGLGLALTKQIVEEHGGTIAITSRVGEGTTVVMRLPVGREKETA"
     gene            5700..7064
                     /locus_tag="AC16BC_08915"
     CDS             5700..7064
                     /locus_tag="AC16BC_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3241780.1"
                     /translation="MTRSLLVVDDERAQRDILARILRDEGYELRTAGTAREAVSIQRK
                     TPCDVVLSDVKMPDTEGVSVLDDLLGAAPNSTVILMTAHGTIDSAVDAMKKGAFDFLT
                     KPLDKDELLITIARAFERVNLLQENQRLRRELDDRVRVGSLIGHHPSMQDIIKVIKKV
                     APSSSTVLIVGESGTGKELIARAIHAESPRRDLPFQAINCAAIPDALMESELFGYEKG
                     AFSGAVARSIGLFEAAHRGTVFLDEIGDLGLGLQAKILRTLQERTIRRVGGQEEIPID
                     VRVVAATNKDLEAEIAAGRFRLDLFYRLNVVTLRVPPLRERAADIPHLVDAFLHKDAG
                     ADRGAKTVSKDALRVLMDYPWPGNVRQLAATIERAVLLCENDEIQVDDLPPEIRRPVS
                     PLPSPFQLPVGGVSLDQLERSLIEQAMAQSNGVIAKAARLLGLSYKTLQYRLEKLRAA
                     GPPT"
BASE COUNT         1320 a         2179 c         2534 g         1298 t
ORIGIN      
        1 ggagtgactt tgatcagtag agaagacgaa aaacttgtca ggtccaggtt acatgaaatc
       61 gcagagatag caaaagagtt tcttattcga acagtttttc ttctacaaca gtccgaaccc
      121 ataccagctt ggtcaggccg ccgaacaagc tctatctcaa tggctgattc gagggctact
      181 ttggttgtca ctgatgagcg tattttgcct acaaaagatt ggaaaataaa acccgagtgg
      241 tgtgattaaa acttctgtcg gtggcctatt cttttcgtgt acccaccacc aattcacgca
      301 agtcacaatg ggagcgccaa gtgacgcaaa ttgcctccct tctctcctaa cccgtccacg
      361 ctttgacacg aagtcggccg tagctatact tgcccgcgtc gcgagggggc tgttggtcgg
      421 cctccgcgcg ttcctgcttg cagcagatca tggtgcacga acccctcaag ccggcgcccg
      481 ctggagccca gccggcaccc gaaaacctct tggtcgagtc ggcccgcgag gtcatgcgcg
      541 cgttggtcaa ggcggtcaag gcgtcgaaga tgtatctgcc ggaacacgag atttgccgga
      601 aatttcggga ggattttgcg gcgcggctct ccggacatct tgaggagcac ggcgatctgg
      661 cgttgaccgt gaaggggggc gagttttggg tgggatcgac gccggtgtat gaagaggcca
      721 accggcacga gaatctcgcg tttcggtgtt cgtcggacgg cctccgggag ctgaccattc
      781 atcgggggct gacgcgggag gacttggatc gactgatcga gattctcacg tccgatgcgc
      841 ggcccttgga caatgacatt gtgactcggc tctgggagca gccgatcccg cacgtgacct
      901 atgaaattgc cgaggtcgat gaggccgaag gcggggaggt cgagttgtcg tcgcggccgc
      961 caagcgaggg ggcgtcgtcc gaagccgctg atgaccgcga ccccggaacc gaggcgccgg
     1021 ctcctgaacc cgaggtggtt cccgagtcgc tgcgtcacga cagcccggtg tttgcgctga
     1081 cggacgagga aatcgcggcc cttcaacggc aagtggtgga agacaccacg caggattacg
     1141 tggggcagtt gatcgatatt ctggagtcga tcctggcgct ggatgacgat gaacagtcgt
     1201 ttctcgaagt gttgcaggtg ttggacgaca tcgtggccac cagcgtgcgg caagggcggt
     1261 tcgagcgggc caacgacgtg gtggcgcggt tgttggcgct tcgggacgat gaggcgcggg
     1321 tctcggcgcg cagccgcgag ttgatccgcg tggtgtggac ccagatgggg acgtgggagc
     1381 gcgtggcgcc gttggaagat ccgttgaacc ggcaaggcgc gtgggaggcg gacgctttgt
     1441 ccgaatatct ggggcagttg ccgtcctcgg cgggcgacgc cttcattacc ctgctcgatc
     1501 acgtgcagac ggccaagggc cgccgaattc tgtgcgacgc gttgaccgcg gtggggggcg
     1561 gagcggtcga gacgatcctg acgcggttgc cggacgcgcc ctggtacgtg gcgcgaaacc
     1621 tcatctatgt tctcgggctc gtgaaggatg ttcgcgcgca gcccgcgttg gaggcggtga
     1681 tgacgcaccc cgacgcgcgg ctgcggaagg aggcgctcag ggccctgggg gacatgggat
     1741 cggaccgcct ggcggccgcg attccccgct ggatcggcga tccggatgaa ggcgtccggt
     1801 tgcaggttct ccgtctggct cgccgccacc cgccgcaggg tttcctcgcg atgttgaccg
     1861 cgctcatcaa tgaccgcgga ttcagtcgcc gtcaggacgc ggagcagcgc gaggtgtttg
     1921 agacgctcgc ggacgtcggg ggtgacgcgg cgctgcccct gatccgctcc tggctcgaga
     1981 cggggcgcgg gtggccgcgg ttctggtccg cgaggcacga tccgcgggcg cggcacgcgg
     2041 tggccgcgtt gcgccggatc gggtcccccg cggccatcgc gcttttacga gagacggtca
     2101 cgtccggacg cggcgtcatc ctcgaagacg cgcgccgcgc cctccacgac ctggagcgcg
     2161 ccgcatgacg ccgcgaggca gcgctccgcg cgtcgccacg cccaagttgt tcgaggcggt
     2221 caagggcagc cacatccggg acgagaatct gcaccggatc ggcaaacgcc tcgtctatca
     2281 agtccatatc ctgctcaaga ccgcgcacat ccacttgacc ggcaacgtgg cgttgagcca
     2341 ggccgcggac cgattggtga agaccgtcgg ggaagtgggg ggctggcggg ccgaacccgt
     2401 gctgcgccag gacggggacc acctgtatct ggatgacatc cggctcaagg cggacctgga
     2461 ggggtacatc ggatttagcg aactgatcgc cgccatgcag gcgcgcgggg tgggaagcct
     2521 gcgattcggc ggcgggctcg gcacggacga ggtgatggcg ttcgcgcacg agtgggccac
     2581 ggccgcgacc acgaccgcgg aggaacttgg cgcggctttg caccgcgtcg gggtgtcgca
     2641 cgtggaggcg gcccctcccc cgtcggcccg ggccaaaccg gcccgcgagc acgagagtca
     2701 gaaagccgtg gccaagcgca cctacttccg gacgatctcc gcggtggcgg aagtcatgga
     2761 gagcgtcaag ctcaaacagg cggtgggcgt ccggaagtcc aaacgcgtgg tccataacct
     2821 ggttgacgtg ttgttgcagg acgagagcac gttgttgggg ttgaccacgc tgcgcagcca
     2881 cgacgaatac acgtacagtc actgcgtgaa cgtgtgcatc ctggccctgt cgatcggcca
     2941 gcggctcggc tacgagcggt cgcatttgtc ggaactcggg gtggccgcgc tgtttcacga
     3001 cgtggggaag gccgacattc cgctggagat cctgaacaag gcgggcgagt tcacgcccga
     3061 agactggcgg gtcatccggc ggcacccggt cttcggggtc aaaagcctgc tgcgtctcaa
     3121 ggggctcgac gatgtctcca ccaagatcgt gatcgcgggg ttcgagcacc acctcaatta
     3181 cgatttgtcg gggtatccga agatcgccat gccccgcaag ctgaccttgt ttggccggat
     3241 catttccctc gcggactgct acgacgccat gaccgcgtcc cgcgtgtaca gccgggtgcc
     3301 gttggcgccg gacaaggcct tgagcctgat ggcggccaag gcgggaaccg tctacgatcc
     3361 gctcctgctc aaggtgttcg ccaacaccat gggcgtgtat ccggtgggga cggtcgtgcg
     3421 gttcgacacc ggggaactcg cggtcgtggt cgggagccat cccgcgcccg aacgacgcgc
     3481 gcatccggtg gtcaagctga ttcgcgatgc gtcgggccgg gacgtggacg gcgagaccat
     3541 cgatctcgcg gacccggacg cccgtccgag ccggcggatc gtggagaccg ccgacgcccg
     3601 atcgctgggc atcgacctcg cccggtactt tctgtaagga tttttacggt ttccccttga
     3661 tttctctgag ggccgtggtg tatctctatc gcggttctcg aaccggtggc atgtgccatt
     3721 ctggaatact tacccgcatc acatggacac gagagagggg aggtgatcgt agtgaagaaa
     3781 ctcgtagtgt tacccttgat tgccgcgctg atgatcctgc cgttgaccgg gtgtgaatcg
     3841 aaaaagctca aagcggagaa cgcgagtctg aaacagcagg tggactcgtt gacccaggag
     3901 aagaacgggt tggcggcgaa ggccgatgag atgaccaagg ccaacgcgga cctccaggcc
     3961 aagatcggtg atctggagaa gaaggtcgag gagttgtcga agccgaaacc ggctccgaag
     4021 cccgcggcca aggcgcccgc caaaaagacc acgaagaagt agtcgagaca tcaacgcgtt
     4081 ttgcgagggg cggacctgac gggtccgccc tttgtttttg tccggtcgac gtccgggggc
     4141 gtgacgcgcg tccggagtcc cgcgccttta catgggctcc cgggcggcag taggatacgg
     4201 ggccggacgg ccatgcaagt ctcgggttcc ctcagtccgc gcgctccgtt tcgcgcgctt
     4261 cagcgcctcc atctcacgac caaactgctg ttggtgatgg gggtgctgac cgtgttgtcc
     4321 ctcgggtcgg tgtttctctt ttacgcctac aacgaacggc tgctgctccg ggagatcacc
     4381 gacaacatca atgatttgtc ctccgcgatc cagatcagcg tggaggagtt gaccagccag
     4441 gagtccacga acgaggggcg tctcaaggac tacgtgaacc ggttggccaa gaagggcgtt
     4501 cgggaaatct cgatcatcag caacgccgag aaggtcatcg cgagttccaa cccgagtcgc
     4561 gtgggccaga gggtcaaccc gtcgcgcaag gacttgttta tcaccgccaa gctcggggag
     4621 gagggcgccc cgggcgcgca ggtccacaac ctgctcgtgc ccatcgtggt gggcaagcaa
     4681 caggtcgggt tcgcccacat catgctggtg atggacgact acgagaacct gttgcggacg
     4741 aatctggtca ggcggctgat ggtgaccgga ggcatcttcg gactcgggat cgggctagcg
     4801 ctctttctgt catggcgcta tacgcggccc atctatcagg tggtggacgt ggccaagcag
     4861 gtggccgcgg gcaatctctc cgcgacgttg ccccaggaac gcggggatga gatcggcgaa
     4921 ctcatcgcga gcgtgaacga gatggtcgat cggctgcgcg agcagcgggt gctggaagac
     4981 cggctgcggg aggccgagcg gttctcggcg ttggggcaat tggcggccgg gatcgcgcac
     5041 gaaatccgca atccgctgaa cttcatcagc ctgtcgctcg atcgcgtgcg ggacgtgatc
     5101 aagcgggacg acggcgcgcg ctccgccgag gtgccggcgc tcctcgggag catcaagggc
     5161 gaggtgttcc ggctcaatcg cctgatcgac ggattcctga gctacgggcg gccggtgagg
     5221 ctcacgcggc ggtgggtctc ggtgcgcggg ctgctcgacg acgtgatcgc cctggtcagg
     5281 cagaaggcgg aagaagccaa ggtcgacctg tcggtggaag gcgccgagcc atcgccgtgt
     5341 tttctggacc ccgaacagat cacgacctgc ttgatgaatg tcatgctcaa cgcctttcag
     5401 tccatgccgg acggcggccg cctgcgcgtg gtcctggacc tggaggcgga cggcaaccgc
     5461 gccgcggagc gattcgtgcg gttgtcggtc gaagacaccg gagccggcat ccctcccgag
     5521 gacctcgcgc gggtgttcga gccgtacttt acgaccaaga agctcggcat cgggttgggg
     5581 ctggcgttga ccaagcagat cgtcgaagaa cacggcggga ccatcgccat caccagccgg
     5641 gtcggcgagg gcaccaccgt ggtgatgcgg ttgccggtcg gacgcgagaa ggagacggcg
     5701 tgacccgttc cttgttggtg gtggacgacg aacgcgcgca acgggacatc ctggcgcgga
     5761 ttctccgcga cgagggctac gagctgcgca cggccgggac cgcgcgggag gccgtgtcga
     5821 tccagcggaa gaccccgtgc gacgtggtgc tcagcgacgt gaaaatgccg gacaccgagg
     5881 gggtgtcggt gttggatgat ctgctcggcg ccgcgccgaa ctcgaccgtc atcctgatga
     5941 ccgcgcacgg gaccatcgac tccgccgtgg acgcgatgaa aaagggggcg ttcgacttcc
     6001 tgaccaaacc attggacaag gacgagttgc tgatcaccat cgcgcgggcg ttcgagcgcg
     6061 tgaacctctt gcaggagaac cagcggctgc gccgggaact ggacgaccgc gtacgggtcg
     6121 gcagtctgat cggtcaccat ccgtcgatgc aggacatcat caaggtgatc aagaaagtgg
     6181 cgcccagttc atcgaccgtg ctgatcgtgg gggagagcgg aaccggcaaa gaattgatcg
     6241 cgcgcgcgat ccacgccgag agcccccgac gggacctgcc gtttcaggcc atcaactgcg
     6301 cggcgatccc cgatgcgctg atggaaagcg aactgttcgg ctacgaaaaa ggggcgttct
     6361 ccggcgcggt cgcccgcagc atcggcctct tcgaggcggc ccaccgcggc acggtttttc
     6421 tcgatgaaat cggcgacctc gggctcggtc tgcaggcgaa aatccttcgg acgctgcagg
     6481 agcggaccat ccgccgggtg ggcgggcagg aggagatccc gatcgacgtg cgggtggtcg
     6541 cggcgaccaa caaagacctt gaagcggaga tcgcggcggg ccgcttccgt ctggatctgt
     6601 tctaccggct caacgtggtg accctccggg tgccgccgtt gcgcgaacgc gccgccgaca
     6661 tcccccacct ggtcgacgcg ttccttcaca aggacgcggg agcggaccgc ggcgccaaga
     6721 ccgtctcgaa ggacgcgctg cgcgtgctga tggactatcc gtggcccggc aacgtgcgtc
     6781 agttggccgc gaccatcgag cgcgcggtgt tgctctgcga gaacgacgag atccaggtgg
     6841 atgatctgcc gccggaaatc cgccggccgg tctctccgct tcccagcccg tttcaacttc
     6901 cggtcggcgg cgtgtcgctc gatcaactcg aacgatccct gatcgaacag gcgatggcgc
     6961 aaagcaacgg ggtcatcgcc aaggccgccc gcctcctcgg attgtcctac aagaccctcc
     7021 aataccggct cgagaaattg cgggccgccg gaccgccgac ctgacaccgc aaatcgggtg
     7081 ggcggcgccg catttcgggt atcgcggggg ccgcgcgttc cgaggatatt cctcaactcc
     7141 tttgtttacc ggggcttttg agcccatcac cgcgctggca cgtcccgtgc tgtagtggac
     7201 tgctccagga agggaggtga tgtgacgtga agcagtggtt caagagcgtg tttgcggtgg
     7261 cggcggtggt ggtgttcgcg gcggcggcgt atgccgggat ggcgacggtg agcggcgagg
     7321 tcaccaagta c
//
LOCUS       DBNGTT010000394        11842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2393720, whole genome shotgun sequence.
ACCESSION   DBNGTT010000394 DBNGTT010000000
VERSION     DBNGTT010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11842
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2393720"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="AC16BC_08920"
     CDS             complement(<1..341)
                     /locus_tag="AC16BC_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S
                     domain-containing protein"
                     /protein_id="HIR3241781.1"
                     /translation="MGVIGNIRRAGAVARLTDPLPEDWRMAPLEEIAEINPRLDKTSL
                     PDDLLVSFVPMAAVGAGDGAIDISNARPFKEVKKGFTPFQEGDVLFAKITPCMENGKM
                     AVVPKLTNGYG"
     gene            complement(332..1774)
                     /locus_tag="AC16BC_08925"
     CDS             complement(332..1774)
                     /locus_tag="AC16BC_08925"
                     /inference="COORDINATES: protein motif:HMM:NF025019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3241782.1"
                     /translation="MKDLVDRAEIIAVLHGFNPWWSGRPVRVPEFRRLAYNACRRYLG
                     HPTLQRAILLSGPRRVGKTTILQQVVDGLIKDAQDPKSILYLSLDHPILKLLSLRNIL
                     AVYHEHIHPEGQPTTLLLDEIQYSREWETEVKLLVDHHPGYRIVATGSASVVHKKRLA
                     ESGVGRWITVPVPTLSFYEFTRLRGEPEPAIDPKLRPGDFFEAAPATLATVAASVRPL
                     LPLFQRYLLVGGFPETAKQDDISLCQRLLREDVVERVLKRDMTALFGVRNVNDLEKLF
                     IYLCLHTGGILAHKTAATALETTTGTVGNHLMLLEQANLVYRLPPIRAGGKKVLKARN
                     KYYLVDAALRNAVLLRGEEVLTNPTEMGLIVETTVLRHLYAYYYRDVPEIAYWRDAVS
                     DREVDIIIRSPKYVLPFEVKYRDKVPLDRSSGLAVFSDAENVKQAYFVTKNETDFGPT
                     KLDGIDTQFLKIPAHVLTYLLGQAERVLWE"
     gene            complement(1935..2207)
                     /locus_tag="AC16BC_08930"
     CDS             complement(1935..2207)
                     /locus_tag="AC16BC_08930"
                     /inference="COORDINATES: protein motif:HMM:NF020052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HIR3241783.1"
                     /translation="MSLVRFATHQENELVPFPERVNANFKSWVGSVEAASRRFAPEQM
                     RWLEMIRDHVAANLSIDVNDFDYAPFGSRAGLARGISSLARTWIRC"
     gene            complement(2313..2540)
                     /locus_tag="AC16BC_08935"
     CDS             complement(2313..2540)
                     /locus_tag="AC16BC_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015140856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HIR3241784.1"
                     /translation="MNESLVTELLKLSPAERIQLAEDLWDSVAAHPESLPPITNEQRT
                     EIERRLSEHRRDPSSAVSLEQVRTRLWSRLG"
     gene            complement(2675..5458)
                     /locus_tag="AC16BC_08940"
     CDS             complement(2675..5458)
                     /locus_tag="AC16BC_08940"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIR3241785.1"
                     /translation="MTTPEDKAREEIDRLLTRAGWIVCNPGDANIVAHHGVAIREFPL
                     KSGHGEADYLFYVGGKAAGVIEAKPEGTTLTGVEVQSAKYTSGLPDALPAWRRPLPFS
                     YQSTGVETRFTNGLDPSPRSRQVFAFHRPEMLARWLDAPYESQAAEKVRTHEGAPATF
                     LAAMQHMPPLEEQGLWRAQINAIKNLERSLAENRSRALIQMATGSGKTFTAITFIYRL
                     IKFAGARRVLFLVDRGNLGDQTLKEFQQYVSPYNNFKFTEEYIVQRLAGNTLDTTARV
                     CISTIQRMYSMLKGRDLPDDLDEQSLDSLGPLFRQPEPIEYNPKIPIETFDIIVTDEC
                     HRSIYNLWAQVLEYFDAYLIGLTATPSKQTFGFFQQNLVMEYGHEQAVADGVNVNYDV
                     YRIRTAITEAGSKVEAGFYVEKRERETRKTRWEELEDDFAYDPNQLDRDVVAPDQIRT
                     VIRTFRDKLSTEIFPGREWIPKTLIFAKDDAHAEEIVQIVRQEFAKGNDFAQKITYRT
                     TGETPQNLIKAFRNSPMPRIAVTVDMIATGTDIKAVEVVMFMRAVKSRAFFEQMKGRG
                     VRVIKPDDLKAVTPDAATKDHFVIVDAVGVCEQDKTDARPMEKKPSVAFDKLLQAVAF
                     GNTEEDVLTSIAARLARMEHRIGQEDEKRIVKASGGLTLKDLSRRLIDAVNPDNVTPS
                     PLAGEGGGEGAALQARIEAVKPLHDPKLRELLIEIKKRDEITIDHISKDRVIEAAFSA
                     DSLDKAKRTVESFEQFIKEHKDEITALQILYSRPYKQRLKFEDVKELADAIEKPPYLW
                     NESQLWQAYAALEKSRVKGASTKRILTDLVSLVRFATHQENELVPFPERVNANFKSWM
                     AQQEQGGKKFTAEQRRWLEMIRDHVAANLAIETSDFDYAPFAQEGGIGKVHQLFGDGL
                     SAIIEELNGALAA"
     gene            5662..6552
                     /locus_tag="AC16BC_08945"
     CDS             5662..6552
                     /locus_tag="AC16BC_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241786.1"
                     /translation="MEPARLWIPGFEPVQFLILLAILFFVGIAVQRVLTAKGRRITMF
                     WQVVSAWIACWAFLTFMLPLPLPFHLRANYLGVITLALFLYAGDTESGWQECCDTVLG
                     MLRGHTRVHRFARVAVFVGLPLVTYAGLHDQMIPRFEAPLEFRAYHPPPPNAFTVQGA
                     TYSDNFSGTMWVRKTEEHPSPALGAAGVTLHIPPHLVELAGGYTHFQIAASGLDVVDV
                     LYQAEAAIPALGSSLHKAPGGSTRGVNVFVLDKDGHFVGDDAYTVGGRSRCCRAKFTT
                     IPPPGSHVDVIPTGSAFTPA"
     gene            complement(6701..6775)
                     /locus_tag="AC16BC_08950"
     tRNA            complement(6701..6775)
                     /locus_tag="AC16BC_08950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6741..6743),aa:Gly,seq:gcc)
     gene            complement(6847..7689)
                     /gene="prmA"
                     /locus_tag="AC16BC_08955"
     CDS             complement(6847..7689)
                     /gene="prmA"
                     /locus_tag="AC16BC_08955"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3241787.1"
                     /translation="MSWISISLRIKPEFAEILGEALIARGSVGVWESQPGWLTAYFPA
                     ASDPRAVKELLEGMSVSFGQGDCLIAPVSDQDWIATWKASVTPLRVTPRLTIVPSWSR
                     YSEEPGEQVVILDPEMAFGTGHHETTRMCLELLDERLQRGDHPAVFDLGTGSGILAIA
                     AAKLGSGRVVACDTDPLACETASRNVEANAVPDTVSVKDAEAGWKSGPYDLIVANLTA
                     EDLCALMPRIAAVLAPNGEAILSGILTSRDSIVNDAVGQAGLQVMQRRQQGEWTALQV
                     GTRR"
     gene            complement(7910..8677)
                     /locus_tag="AC16BC_08960"
     CDS             complement(7910..8677)
                     /locus_tag="AC16BC_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241788.1"
                     /translation="MSPYLQETTAMTRRWFQRFRREPMSIIFGLTQPVLWLVLFGNLF
                     KNATAITGLQAPSYIAFMTGGVVVMTVLNSGLAGGIEILFDKENGFLERLMAAPIKRS
                     SLIISRFLFVTALTGLQALMILGMAFLFGVVPATGWPGVILILVIGMMLGIGFTAISL
                     GLAFSMKGHGGFFALLGFITLPLTFLSSALAPTEIMPGWLALIAHLNPMTYAIDAVRG
                     LILTGWHIHIIKVLVILAVFDAVCLAWSNRILLRGMR"
     gene            complement(8791..9753)
                     /locus_tag="AC16BC_08965"
     CDS             complement(8791..9753)
                     /locus_tag="AC16BC_08965"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3241789.1"
                     /translation="MSGPSIITAANLVKRFGKTTAVDGLSLEMYEGEVFGLLGPNGAG
                     KTTTMRMLITLLQPDGGDITVGGYKVRENAERVRSLIGYIPQERAIDRALTGREHLAL
                     FADLYHLPKAEGLERAEWALKLVGLEDRADEVVMNYSGGMKKRLEIACGLIHRPKILF
                     LDEPTLGLDVESRTLIWRHIRELKAQGMTMVMSTNYLDEADQLCDRLAIIAQGKLAAL
                     GTPRQLKESLQGDVLLLTPEGADTAALERLAISLKGLEGVVNVVRRDGSLELRVTPSR
                     AILNRVLDAVASQGARLEAIQYTRPSLEKVFIHYTGRHIKDVWE"
     gene            complement(9750..10592)
                     /locus_tag="AC16BC_08970"
     CDS             complement(9750..10592)
                     /locus_tag="AC16BC_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HIR3241790.1"
                     /translation="MTTPISQDRWSAARGPRRIVCLTPESAEILYALGAGERVVGVSG
                     YTVQPPEARRKPKVGAYTTVDFDRVMGLDPDLVVAYSDLQADITAALARRGATVLHLN
                     QRTFDGLCHAVELLGGVVGEPAAAAKLIGLIRGECASASRAARALPRRPRVYFEEWDD
                     PLIAGIGWVSEAVAIAGGEDVFADLNRTGAAKDRVVSADDVRRRNPDIVFASWCGKKV
                     RPERIASRPGWRDIAAVRDGHVYEIKSAYILQPGPSVLTGVRQMAERIAAWSAAASGA
                     ARPS"
     gene            11148..11675
                     /locus_tag="AC16BC_08975"
     CDS             11148..11675
                     /locus_tag="AC16BC_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241791.1"
                     /translation="MSPAPTETPAPAPAPKETPGAAPETPPAPPPALKDRPYFLRAAL
                     QTRGDDKSRVGVGVSAEMRSPLPWDLLGGPGIELDLFQSQSDSNAVSFLPVYWGVEYR
                     PLQRFPHAFFAGRLGFDMLGQEGDDTMASRTFYAASIGLVTHLNRAKAMQWELIYSRM
                     RGAFPGIGLSVGYRY"
BASE COUNT         2057 a         3845 c         3646 g         2294 t
ORIGIN      
        1 aaaccatacc cattcgtgag cttcggcact actgccatct tgccgttctc catgcagggc
       61 gtgatcttcg caaacaacac gtcgccctct tggaacggcg tgaatccttt cttgacctcc
      121 ttgaacggcc tggcgttgct gatgtcgatt gccccatcgc ctgcccctac tgcagccatg
      181 gggacaaacg agaccaacag atcatctggt aacgacgtct tgtcgagacg cgggttgatc
      241 tcggcgatct cttccagtgg cgccatcctc caatcttcgg gtaatggatc cgtcaagcgt
      301 gcgacggcac cagccctccg gatgttgccg atcactccca caggactcgc tccgcctgac
      361 caagcagata ggtcaacacg tgcgcgggga tcttgaggaa ttgagtatcg atcccgtcga
      421 gtttggtggg gccgaagtcc gtttcgttct tggtcacgaa gtacgcctgc ttcacgtttt
      481 cggcgtcgct gaaaacagcg aggccgcttg agcgatcaag cggtaccttg tctcggtact
      541 tcacttcgaa aggcaacacg tatttggggc tgcggatgat gatgtccacc tcccggtcgc
      601 tgaccgcgtc acgccaatac gcgatttcgg gaacgtctcg gtagtaatat gcgtagagat
      661 gtcgcagcac cgtggtctcg acgatcaatc ccatctcagt tgggttggtg agcacctcct
      721 ccccgcggag cagcacggcg ttgcgcaacg cggcgtcgac caggtagtac ttgttgcgag
      781 ctttgaggac tttctttccc ccggcgcgga tcggcggcaa tcgatacacc aggttcgcct
      841 gttcgagcag catgaggtgg ttccccacgg tgccggtcgt ggtctccagg gccgtggcgg
      901 ccgtcttgtg cgcgagaata ccgccggtgt gcaggcagag atagatgaac aacttctcaa
      961 ggtcgttgac gttgcgtacg ccaaagagcg cagtcatatc gcgcttgagc acacgctcga
     1021 ccacgtcctc gcgtaagagg cgctgacaca aactgatatc atcctgcttg gcggtctccg
     1081 ggaacccgcc gaccagcaga tagcgttgga agaggggcaa cagcggccgc acgcttgctg
     1141 ccaccgtggc cagggtcgct ggggcggcct cgaagaaatc gccgggacga agcttgggat
     1201 cgatcgccgg ttccggctcg ccgcgcagac gggtgaattc gtagaacgac agtgtgggaa
     1261 ccggcaccgt aatccagcgc cccactccac tttcggcaag ccgtttctta tgaaccacgc
     1321 tggccgatcc cgtggccacg atgcgatagc ccgggtggtg gtctacgagc agcttgacct
     1381 cggtttccca ctcgcgggag tattgaatct catccaacaa caacgtagtc ggctgtccct
     1441 caggatggat gtgctcgtgg tacaccgcga gaatatttcg caacgagagg agtttgagga
     1501 tcggatgatc caggctcaaa tacaggatgc tcttggggtc ctgagcgtcc ttgatgagcc
     1561 cgtcaacgac ctgttgtaga atcgtcgtct tgccgacccg tctgggacca gagagaagga
     1621 tggcgcgctg caacgtgggg tgcccgagat agcgacgaca ggcgttgtag gcgagacgcc
     1681 ggaactcagg gacacgaacc ggcctcccac tccaccacgg attaaaaccg tgcagaaccg
     1741 cgataatctc cgcccggtca acaagatctt tcaattctcg ttcctcactc gataaaaagt
     1801 actttttgtc aataagtgca taacatacta tgcactcacg tcatataaaa tcaagttatt
     1861 gagatgctaa tggaactttg tggatggtgg tcagtgaatc gccttctatg ctgccaaagc
     1921 ctcgttcaat tcgctcagca tctgatccag gtccttgcca aagagctgat gccccttgcc
     1981 aatcccgccc tgctgccgaa tggcgcatag tcgaagtcgt ttacgtcaat tgagagattc
     2041 gccgcgacgt ggtccctgat catttccagc cagcgcattt gctcgggggc aaagcggcgt
     2101 gacgccgctt ctacgctgcc aacccatgac ttgaagttcg cattgacccg ctcaggaaac
     2161 ggaaccaatt cattttcttg atgcgtcgcg aaccggacta acgacaccaa gtctgcggcg
     2221 tgcggcgccg agaccgcgcc cgtgggcttc gtaccagtcg accgcctcag cgagttcggc
     2281 ctcgacctcg ggccggaaga ccaggcgtcg cgtcacccga ggcgcgacca gagtcgcgtg
     2341 cgcacctgct ccaaactgac cgccgaactg ggatcgcgcc ggtgttcaga caggcggcgc
     2401 tcgatctcgg tgcgctgctc gttcgtgata ggagggaggc tttcggggtg ggctgcgacg
     2461 ctatcccaaa gatcttcggc aagctgaata cgctcggcag gggacagctt gagcaattcc
     2521 gtaacgagag attcgttcat catctcctcc gtacgttcca cggtgcatag gggtgtcgct
     2581 ggtacgctga ttgtacatca gcaccatctc tgtttccatc aagcaccctt ctttcgccat
     2641 cccttgtcac agtgagggga tggatggggg gagcttatgc cgccagtgcc ccgttcaact
     2701 cttcaatgat cgcactcagc ccgtcgccga acagttgatg gaccttgccg atcccaccct
     2761 cctgcgcaaa cggcgcatag tcgaagtcgc tcgtttcgat tgccagattc gcggcaacgt
     2821 gatcgcggat catttccaac cagcgacgct gctctgccgt gaatttcttc ccgccctgtt
     2881 cctgctgcgc catccacgat ttgaagttcg cattcacccg ctcgggaaac gggaccaact
     2941 cattctcctg gtgcgtggcg aatcggacca aggacacgag atcggtgaga atccgcttgg
     3001 tgctcgcgcc tttaacccga gacttctcca gcgccgcata cgcctgccag agctgcgatt
     3061 cgttccacag gtacggcggc ttctcgatcg cgtccgcgag ttctttgacg tcttcgaact
     3121 tgagccgctg tttgtacggt cggctataga ggatttgcag cgcggtgatt tcgtccttgt
     3181 gctctttgat gaattgctcg aacgactcga ctgttctctt cgctttgtcc aacgaatcgg
     3241 ccgaaaacgc ggcttcgatc acccgatcct tgctgatgtg gtcgatggtg atttcgtcgc
     3301 gcttcttgat ttcgatcagc aactcgcgga gtttggggtc gtgcaggggt ttgaccgctt
     3361 cgatccgcgc ttgcagcgcg gccccctcac ccccgccctc tcccgcaagg ggcgagggag
     3421 tcacgttgtc gggattgacc gcgtcgatca accggcggct caggtctttg agcgtcaggc
     3481 cgccgctggc tttgacgatg cgcttctcgt cctcttggcc gatccggtgc tccatgcgcg
     3541 cgaggcgcgc ggcgatcgac gtgagcacgt cttcttcggt gttgccgaac gccacggctt
     3601 ggaggagctt gtcgaacgcc acgctcggct ttttctccat gggccgggca tcggtcttgt
     3661 cctgctcgca caccccgacc gcgtccacga tcacgaagtg gtctttggtg gcggcatcgg
     3721 gcgtgacggc tttgaggtcg tcgggcttga tcacgcgcac gccgcggcct ttcatctgct
     3781 cgaagaaggc gcgggatttg acggcgcgca tgaacatgac cacttccacc gccttgatgt
     3841 cggtgccggt cgcgatcatg tccaccgtca cggcgatgcg cggcattggg ctgttgcgga
     3901 aagctttgat gaggttctgc ggcgtctcac ccgtggtccg gtacgtgatc ttttgggcga
     3961 agtcattccc tttggcgaat tcctggcgca cgatctgcac gatctcttca gcgtgggcgt
     4021 cgtccttggc gaaaatgagg gtcttgggta tccactcgcg gcccgggaaa atctcggtgg
     4081 atagcttgtc gcgaaacgtc cgaatgacgg tgcggatctg gtccggcgcg accacgtcgc
     4141 ggtcgagctg gttggggtcg tacgcgaagt cgtcctccaa ctcctcccaa cgcgtcttgc
     4201 gggtctcgcg ctcgcgcttt tccacgtaaa agcccgcctc caccttggac ccggcctcgg
     4261 tgatggcggt gcggatgcgg tagacgtcgt agttgacgtt cacgccgtcg gccacggcct
     4321 gttcgtggcc gtactccatc accaggttct gctggaagaa accgaaggtc tgcttgctcg
     4381 gcgtggcggt gaggccgatg aggtaggcgt cgaaatactc cagcacctgc gcccagaggt
     4441 tgtagatgga gcgatggcac tcgtcggtca cgatgatgtc gaacgtctcg atggggatct
     4501 tggggttgta ctcgatcggc tcgggctgcc tgaagagcgg cccgaggctg tcgagggatt
     4561 gctcgtcgag atcgtccggg aggtcgcggc ccttgagcat ggagtacatg cgctggatgg
     4621 tgctgatgca gacccgggcg gtggtgtcga gcgtgttgcc ggcgaggcgc tggacaatgt
     4681 actcctcggt gaatttgaag ttgttgtagg gcgagacgta ctgctggaac tccttgaggg
     4741 tctggtcgcc gaggttgccc cggtccacca ggaacaggac gcgccgcgcg cccgcgaact
     4801 tgatgagccg gtagatgaac gtgatggcgg tgaaggtctt gcccgagccg gtggccattt
     4861 ggatgagggc gcgcgagcgg ttctcggcca gggagcgttc gagattcttg atggcgttga
     4921 tctgggcgcg ccaaaggcct tgttcttcga ggggcggcat gtgctgcatg gccgccagaa
     4981 atgtggcggg agcaccctcg tgcgtgcgga ccttttccgc cgcttggctt tcgtacggcg
     5041 cgtcgagcca tctggcgagc atctcgggcc gatggaaggc aaacacctgc cgcgaacgcg
     5101 gcgacgggtc caggccgttg gtgaagcgcg tttccacgcc cgtggactga tacgaaaatg
     5161 ggagcggacg gcgccacgcc ggcagcgcgt ccggcaggcc cgaggtgtac ttggccgact
     5221 gcacttcgac cccggtgagc gtcgtgccct cgggtttggc ctcgatcact cccgccgcct
     5281 taccgcccac gtagaacaag taatcggcct ctccatggcc cgacttcagc gggaactccc
     5341 gaatcgccac gccgtgatgc gccacgatat tggcgtcacc cggattgcag acgatccaac
     5401 ccgcccgcgt cagtaaacga tcgatctctt cccgcgcctt gtcctcgggg gtggtcatgg
     5461 caagcacgac cccgaaccga tagctgttgt gggacggctc tcaaacaagt gccaggcccc
     5521 tcgtagattt ccttgcggtg gaggcccagc ttagcccgtt gcgggctctc gaatcaagac
     5581 actcccccgc cgtccccctt ttgacacgca cctcgttcca ggcgtagagt gggttcacca
     5641 catcccaccc gggagggcca catggagcct gctcgcctct ggattccggg attcgagcct
     5701 gttcagttcc tgattctgct cgcgatcttg ttcttcgtcg gcattgcggt tcaacgggtg
     5761 ctcacggcca agggccggcg catcaccatg ttctggcagg tggtgtcggc gtggatcgcg
     5821 tgttgggcct tcctgacgtt tatgctgccc ttgccgctgc cgttccacct ccgggcgaac
     5881 tacctcggcg tgatcacgct ggcattgttc ctgtacgcgg gggacacgga atcggggtgg
     5941 caggagtgtt gcgacacggt gctgggcatg ctccgcggcc acacccgcgt gcaccggttc
     6001 gccagagtag cggtgttcgt cgggctgccg ctcgtcacct acgcggggct ccatgaccag
     6061 atgatcccac ggttcgaagc gccccttgag ttccgtgcgt accatccccc gccgccgaac
     6121 gcgttcacgg tgcagggggc gacatattcc gataacttct ccgggacgat gtgggtgcgc
     6181 aaaaccgagg agcatccgtc gccggcactt ggcgccgcag gcgtcaccct ccacatcccg
     6241 ccgcatctgg tggagctggc cggcgggtac acgcactttc agatcgcggc gtcagggctc
     6301 gacgtcgtcg acgtgttgta ccaggctgag gccgcgattc cggccttggg aagttcgctg
     6361 cacaaggctc cgggagggtc cacccggggc gtgaacgtct tcgtgctcga caaggacggg
     6421 cacttcgtcg gagacgatgc gtataccgtc ggggggcgat cccgctgctg cagggcgaag
     6481 ttcacaacca tcccgccccc gggaagccac gtcgatgtca tccccacggg ctccgcgttc
     6541 acgcccgcgt aggggaccaa catccgcttg agcgcggcga gatccgcgtg gggggcgagc
     6601 cagcggcact ggtccagggg cgcgagaatg tgatggcggc gcgaagcgcc cactcctcgg
     6661 gagcaaagcc cgaacatccc gcgacattga cgtgaggaaa tggagcggga aacgggattt
     6721 gaacccgcga ccctcgcctt ggcaaggcga tgctctaccg ctgagctatt cccgcttgtg
     6781 tgacgccgcg cggcgcgaaa ctcgccgcgc acagccggga ttttttatca cggccggtaa
     6841 gggaagtcaa cgtcgcgtcc cgacctgcaa cgcggtccac tccccttgct gccggcgttg
     6901 catgacctgc aggcccgcct gtccgaccgc gtcgttcacg atggagtcgc gcgacgtgag
     6961 aatgccggac aggatcgctt cgccgttggg cgcgagcacg gccgcgatgc ggggcatcaa
     7021 ggcgcacagg tcttccgcgg tcaggttggc cacgatcaga tcgtacggcc cggatttcca
     7081 tcccgcctcc gcgtccttca cggacaccgt gtcagggacc gcgttggctt ccacgttgcg
     7141 gctcgcggtt tcgcacgcga gcgggtccgt gtcgcaggcc accacgcggc ccgatcccaa
     7201 cttggccgcg gcaatggcca ggatcccgga acccgtgccc aggtcgaaca ccgcggggtg
     7261 atccccgcgc tgcaagcgct cgtccagcaa ctccaggcac atccgcgtgg tctcgtggtg
     7321 cccggtgccg aacgccatct ccgggtccag aatcaccacc tgctcgccag gctcctctga
     7381 ataccgagac cagctcggca caatggtgag gcgcggcgtg acgcgcaacg gcgtcacgct
     7441 cgccttccac gtcgcgatcc aatcctgatc gctgaccgga gcgatcagac aatcgccttg
     7501 tccgaacgat accgacattc cctcaagtaa ttctttaacc gctctcgggt ccgacgcggc
     7561 cggaaaatac gcggtgagcc agccaggctg agactcccaa acccccacac tcccccgcgc
     7621 aatcaacgcc tccccgagga tctcggcaaa ctccggcttg atgcgaagcg atatggaaat
     7681 ccaagacatg gggtgtgttt gttttgtttt tatcttaacc gtgtccgagg ttaccggaca
     7741 cggagcctgt tcaggaaggt gtcagatgcc cccttcgggg acttccggca tttgattttt
     7801 tggcggtggg tcgcaaggcg gagcgagcgc ccgcaccgga gcgtactgtg tcgtacgtga
     7861 ggcgcggacg cgcagcgaca acgcagcaga tgacccttca tggacaggct caccgcatgc
     7921 cgcgcaagag gatgcgatta ctccaggcga gacacaccgc atcaaacacc gccagaatca
     7981 ccagcacttt gatgatgtgg atgtgccagc ccgtgaggat cagcccgcgg accgcgtcga
     8041 tggcgtacgt catgggattg aggtgggcga tcagcgccag ccaccccggc atgatctcgg
     8101 tcggcgccag cgcgctgctc aggaacgtga ggggcagggt gatgaagccc agcagcgcga
     8161 aaaacccgcc gtgccctttc atggaaaacg ccaggccgag cgaaatcgcg gtgaagccga
     8221 tgcccagcat catgccgatc accagaatga ggatcacgcc cggccatccg gtcgcgggca
     8281 ccacgccgaa gagaaacgcc atgcccagga tcatgagcgc ctgaagcccg gtgagcgcgg
     8341 tcacgaagag aaagcggctg atgatcaacg agctgcgctt gatgggcgcg gccatcagac
     8401 gctcgaggaa cccgttctcc ttgtcgaaga ggatttcgat gccgccggcg agcccgctgt
     8461 tcagcacggt catcaccacc accccgccgg tcatgaatgc gatgtaactc ggcgcctgga
     8521 gacccgtgat ggccgtcgcg ttcttgaaca ggttcccgaa cagcaccagc cacaacaccg
     8581 gctgggtcag cccgaagata atgctcatgg gctcgcgccg gaaccgctgg aaccagcgcc
     8641 gggtcatggc tgtggtttct tgaaggtacg gtgacatcgt gtgtcctgtt cgcctattct
     8701 ccggttcccc ggctcaccgg ttcgtcctta ctccgattcc ccgcttcccc gattcgccct
     8761 tactccgata ccccggctca atccctatcc ctactcccac acgtccttga tgtgcctgcc
     8821 cgtgtaatgg atgaacacct tctccaggct cggccgcgtg tactgaatcg cctcaagacg
     8881 ggcgccctgc gacgccaccg catccaagac gcgattgagg atcgcgcgcg acggggtgac
     8941 gcgcagttcc agcgagccgt cgcggcgcac cacattcacc acgccttcca gtcccttgag
     9001 ggagatggcc aggcgttcga gcgccgcggt gtccgcgcct tccggcgtga gcaggagcac
     9061 gtcgccctgc aggctctctt tgagttgccg cggggtaccc agcgcggcca atttgccttg
     9121 cgcgatgatg gccaggcggt cgcacaactg gtccgcttcg tcgaggtagt tcgtgctcat
     9181 caccatggtc atgccctgcg ccttgagctc ccggatgtgg cgccagatca gggtgcggct
     9241 ctccacgtcc aggcccaggg tgggctcgtc cagaaacagg atcttgggcc ggtggatcag
     9301 cccgcacgcg atctccaggc gcttcttcat gccgccggag tagttcatca ccacttcgtc
     9361 cgcgcggtct tccagcccca ccagcttcaa cgcccattcc gcccgttcga gtccctcggc
     9421 cttgggcaga tgatacaggt ccgcgaacaa cgcgaggtgc tcgcgcccgg tgagcgcgcg
     9481 gtcgatggcg cgctcctgcg ggatgtagcc gatcaggctc cgcacgcgct ccgcgttctc
     9541 ccgaaccttg tacccgccca ccgtgatgtc gcccccgtcc ggttgcagga gcgtgatcag
     9601 catgcgcatg gtggtggttt tgcccgcgcc gttggggccc agcaggccga agacctcgcc
     9661 ctcgtacatt tcgagggaca acccgtccac cgccgtggtt ttgccgaacc gcttgaccag
     9721 gttggcggcg gtgatgatgg aaggcccgct cacgagggac gcgccgctcc cgaagccgcg
     9781 gccgaccacg ccgcgatgcg ctcggccatt tgccgcacgc cggtcagcac gctcgggccg
     9841 ggctggagaa tgtacgcgga cttgatctcg tacacgtgcc cgtcgcgcac cgccgcgatg
     9901 tcccgccagc ccggccgcga cgcgatgcgc tcgggccgga ctttcttgcc gcaccacgag
     9961 gcgaacacga tgtccgggtt gcggcggcgc acgtcgtccg cggacaccac gcggtccttc
    10021 gccgcgccgg tgcggttcag atccgcgaac acgtcctcgc cgcccgcgat ggccacggct
    10081 tcgctgaccc aaccgatgcc cgcgatcaac ggatcgtccc actcctcgaa gtacacgcgc
    10141 gggcgtcggg gcaaggcccg cgcggcgcgg ctggccgacg cgcactcgcc gcggatcagc
    10201 ccgatcaact tcgcggcggc cgcgggttcg ccgaccacgc cgcccagcag ttcgacggcg
    10261 tggcacaggc cgtcgaacgt ccgttggttc aggtgcagga cggtggctcc gcgccgggcc
    10321 aacgccgcgg tgatgtcggc ctgcaagtcc gagtacgcca ccaccagatc cgggtcgagc
    10381 cccatcaccc ggtcgaaatc cacggtggtg tacgcgccca ctttcggctt gcgacgggcc
    10441 tcgggcggtt gcacggtgta cccgctgacg ccgaccacgc gctcgcccgc gcccagcgcg
    10501 tacaggatct ccgcgctctc cggcgtgagg cacacgatgc gtcgcggccc gcgggccgcg
    10561 ctccagcgat cttgagagat aggcgttgtc atcagaatga cgaggtaatc ccccgtcagg
    10621 agcgcgagat tataacaccg cgccgatggc acctcaagga ggaaaactcg cgctcgcgac
    10681 ccgcagccgg cgcgggatat cccgccaact aggcaacccg ctgtcgactg tgctaggctg
    10741 ggatgatttt cgcaaggccc gatgtccagt aggagggcct tttgtccgtc ttgctcgtga
    10801 ccggtcacga aatcatcaac gactcgacat cccaggcgtc gccaccacga cgtcccgctc
    10861 agcggttctc ccgccccgcg gccgcggtcc tcgcgctgag cgcgtggatg gtcggcgccg
    10921 gagcctgggc ccaggaaccg gatacaccgg cgccggagcc tccggcaaac gaggccccga
    10981 cccagccgcc ccaatccgaa ccagcgccac cggccgagtc accggcgccg caagagccga
    11041 cgcccgcgcc gccggtgtcc caggagccgt ccatccctgc gccggctccg caagaaaccc
    11101 cggctacgcc gccggctccc cgggaaatac cgcccgcgcc gccgaccgtg tcacccgcac
    11161 ccacggaaac tccggcgccc gcaccggctc cgaaagaaac accgggggct gcgccggaga
    11221 caccgcctgc gccgcccccc gcgctcaagg accggccgta tttcctgcgc gccgcgctgc
    11281 aaacgcgcgg tgacgacaag tcgcgcgtcg gggtcggggt gtccgcggaa atgcgttccc
    11341 cgctcccctg ggacttgctc ggcggtcccg ggatcgaact cgacctgttt cagtcccaaa
    11401 gcgattccaa cgccgtcagc tttcttcccg tgtattgggg cgtggagtat cgtccgctgc
    11461 aacgatttcc gcacgcgttt ttcgccggcc ggctcgggtt cgacatgctg ggccaggagg
    11521 gcgacgacac catggcgtcg cgcacgtttt acgccgcgag catcggcctg gtcacgcacc
    11581 tgaaccgggc caaagccatg cagtgggaac tgatctactc gcgcatgcgg ggcgcgtttc
    11641 cgggaattgg actgtcggta ggctaccggt actaagttca tcgaggctgc tgaaaacgcc
    11701 cacctgctgc gttctcggtc gtcggaaatc ctcacgtacg actcagtacg ctccgggttc
    11761 ctccttcctg cggccttgca gctgggacgt tttgagcagc ctccggctcc gccgaattat
    11821 tcagacggtt gtcaatctcc ag
//
LOCUS       DBNGTT010000395         4787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1499396, whole genome shotgun sequence.
ACCESSION   DBNGTT010000395 DBNGTT010000000
VERSION     DBNGTT010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1499396"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(430..927)
                     /locus_tag="AC16BC_08980"
     CDS             complement(430..927)
                     /locus_tag="AC16BC_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HIR3241792.1"
                     /translation="MKGRRDAARLAAREQGLHERMGRAAHALLSDGVSIERTPDFNAM
                     LAELGQLATRQRNRMHGAHSEQAPLSPSVSVRRLTRALQAGDWRVHTMAIEASSPWLG
                     KTLDEEPPAGLCLAVQRDRTLFPFSADWTFQQADLLLVLAPVSHLALWENWMAKGSTD
                     YGDAL"
     gene            complement(1087..2271)
                     /locus_tag="AC16BC_08985"
     CDS             complement(1087..2271)
                     /locus_tag="AC16BC_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR3241793.1"
                     /translation="MSLEGSIKDFGLSDIFQLIYVQQKSGLMSIEDGARRAGVGFVKG
                     MVVSAQMDRSEGIERLGEVLVRSKRITRAQLDRALKAQEESGENIGKILVSQQSVSEE
                     DLKKALRLQTLETVYRLFRWKEGRYSFDQRDIDYPKQYIDPISTEHILMEGVRRLDEW
                     PPIEKRIPSLQIVFKQVPEKWAEIEQADPPPAPAKSAEDDPFADLDPEPEGRFSAHEI
                     SVYHGVNGSHTVSELIELLQIGEFEVCKALANLLNAGLIVPLNVPSIAETTEQPGRLS
                     ELGKYASTALFWMINVALTAGLIGGPAWLFWNARDTLARIVGDESVTMNVVLLPNQIK
                     GLREIVEVWKAEYGAFPTSVFDPLASYGAAAWPLKDPWGNPWSYDQNTGKVGVGSKSP
                     AK"
     gene            complement(2278..4137)
                     /locus_tag="AC16BC_08990"
     CDS             complement(2278..4137)
                     /locus_tag="AC16BC_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241794.1"
                     /translation="MHLWAATVRTLVVTLSFVSPVIAATDVDPQAAWNAYRATVDSAA
                     ESGEIPVAARAVADAARNGHIQQYPAWSLVIVRDADRLLKRGAREEAIELAQIASSLA
                     PELSSPHIWLASALLSGPSPDALASGKEWVAALNASYRDFWSLLYRIDRWVAAAVLSL
                     LSAVVVVILVLVIRVIPLLAHLFVEWSGHRIFRPTAWLAALWLLIAPLAMVQWSAWYV
                     MVPGAALWWFLSRRERVVLGGLAALGILASLLIPRVVPILTADENAEFRLVVDVAEGR
                     DPAVALADSEIVDSADGAVARATALMRAGRTDEAVTLSQEALVRWPNDPRLLTGYGNI
                     LFRRQDYGHAISAYEQALAVVPNAVPVLYNLSQAYRADLKFDEGEVKYQAARVINAGL
                     LDRYGELSARGADFAVVDYPTTTEELLTDALRSPPASPVIQDSVTKVARHLSPAATIG
                     IAVLLAACWGLGRWFPSQAASPCEICGGAICRRCQRYFLDLKLCTACWKSYARNVKLA
                     PRSTLPQVLHRWDVRRRVAAVLSVIPGAGHALLGRPFWGVIFALAGWWVLWIGILQDV
                     SWNTADARIVPAPWYVMWGPVVGGFAVIGLLGARHLLTMNWTPAASSLPEDRR"
     gene            complement(4137..>4787)
                     /gene="plsY"
                     /locus_tag="AC16BC_08995"
     CDS             complement(4137..>4787)
                     /gene="plsY"
                     /locus_tag="AC16BC_08995"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR3241795.1"
                     /translation="GLSPLARPDFRDMTADSSLLVGLAIVLFAYMIGTIPFGVVVARW
                     IGASDPRTKGSGNIGSTNVLRVAGKGPAAATLLLDAGKGAVAVWAASLLTADQTARWA
                     EISGMAVVAGHTFPVWTGFRGGKGVATGIGALAALAPITAGGVLLAWITVLALFRYVS
                     LASITAAASLPIAVFALGPSRLRVFSVVVAAVIVIRHQENIRRLLAGTESPVGRRV"
BASE COUNT          960 a         1547 c         1442 g          838 t
ORIGIN      
        1 tcgaagaact cgcggcgttg gcctcggacc tcaagcggtt aatcgcccga tagataagga
       61 ttgattctcg aattataggg aagtcccggt ctgcccggtc accgcgtcaa cgccgtgact
      121 gggcagacgt gtttctagcc gcgtaaaagc ccgaatggga ataatcatcc agtacttgat
      181 gtttatatct caagggggct tggcggccaa tgagtcggat tctcctgcag tggtgggggc
      241 gttcgttgat taggattaag ctccagtggc ttgccctggc tggataccgc ttggaacgaa
      301 ggatcgaggg tcggaagcgc gactcgatcc gatctcactg aaaaatcacg cagaaacctg
      361 atcaaaccca aagccgtctc gtcggacgcc gatgaattgt gacgtccgcg cgcttagcaa
      421 cagggttgtt cacagcgcat ccccataatc tgtggaaccc tttgccatcc agttctccca
      481 taaggcaagg tgcgaaaccg gagcgagaac cagcagaagg tccgcctgct gaaacgtcca
      541 gtccgcgctg aacggaaaga gggtgcggtc tcgctggacc gcgaggcaga gtccggccgg
      601 cggctcttcg tccagcgtct tcccaagcca cggagacgac gcctcgatag ccatggtatg
      661 gacccgccaa tctccggctt ggagggctcg cgtgaggcgg cgcaccgaga cggacggact
      721 gagcggtgcc tgttcggaat gagcgccgtg catccggttc cgctggcgtg ttgccagttg
      781 accgagctcc gccagcatgg cgttgaagtc aggggtacgc tcgatcgaca cgccgtcgct
      841 gagcaacgcg tgcgcggctc gccccatccg ctcgtgcagt ccctgttcgc gcgccgccag
      901 ccgggcggcg tcacgccgac ctttcatctg cgatcgcaca tcctccgcaa gatcagcgac
      961 ctcccagagg atttcgcgac aggcgtgagc gagtacggaa atatcgtcga tgagttgggc
     1021 agccaatcgg ggcattttat gtgtccgata agaaatattc tgtaaaatta tgatttgagc
     1081 gcatacttat tttgcggggc ttttcgaacc aacccccacc ttgcctgtgt tctggtcata
     1141 actccaggga ttgccccagg gatccttgag cggccaggcc gccgcgccat aactcgccaa
     1201 cggatcaaac accgaggtgg gaaatgcgcc gtattcagcc ttccagacct cgacaatttc
     1261 tctcagtccc ttgatctgat tgggaagcag cacgacgttc atcgtcaccg actcatcccc
     1321 gacaattcga gccagcgtgt ctcgcgcatt ccagaataac caggccggcc ccccaatcag
     1381 tccggcggtc aaggcgacgt ttatcatcca gaacagggcc gtcgacgcgt atttgcccag
     1441 ctcagacagc ctgccgggtt gctcagtcgt ctccgcgatc gacggcacgt tgagcggaac
     1501 aatgagcccg gcgttcaaga gattggccag ggccttgcaa acctcgaatt ctccaatttg
     1561 gagcagttcg atcagttcag agacggtatg ggagccgttg accccatgat acacgctgat
     1621 ttcgtgcgcc gaaaaccgcc cttcgggctc cggatccaaa tcggcaaacg ggtcgtcttc
     1681 cgcggatttc gcgggagcag gcggcgggtc ggcctgttca atctccgccc acttttccgg
     1741 aacctgcttg aacacgatct ggagcgacgg gatgcgtttc tcgatgggcg gccattcatc
     1801 gagacgacgt actccctcca ttaagatgtg ttcggtgctg atcgggtcga tgtactgttt
     1861 ggggtaatcg atgtcccgct gatcaaacga gtagcgccct tccttccatc ggaacaatcg
     1921 atacacggtt tcgagggttt gcagccttaa agcttttttg aggtcttctt cgctaacaga
     1981 ttgctgtgaa acaagaattt tcccgatatt ttctcctgat tcctcctgag cctttagcgc
     2041 ccgatcgagt tgtgcccggg taatccgctt ggagcgaacc agaacctctc cgagtcgctc
     2101 aatcccctca ctccggtcca tctgcgccga aaccaccatg cccttgacga agcccacgcc
     2161 ggcccggcgc gccccgtctt caatggacat gaggccgctc ttttgctgga cgtaaatgag
     2221 ctggaagatg tccgagagtc cgaagtcctt gatcgatcct tcgagcgaca tcgacggtta
     2281 cctgcggtcc tccggcagtg atgaggccgc cggggtccag ttcatggtca aaagatggcg
     2341 agcgccgagc agtccgatca ccgcgaagcc cccgaccacc gggccccaca tgacgtacca
     2401 gggcgcgggg acgattcgcg cgtccgcggt gttccaactc acgtcctgga ggatgccgat
     2461 ccaaaggacc caccatcccg ccaaggcgaa gatcaccccc cagaacggcc gtccaagcaa
     2521 cgcgtgcccc gcgccgggaa tgacggagag gacggccgcc acgcgacgcc ggacgtccca
     2581 ccggtgcagg acttgcggaa gggttgaacg aggcgcgagc ttgacgttcc gtgcgtacga
     2641 tttccagcac gccgtgcaca atttgagatc caggaagtac cgttgacagc gccgacaaat
     2701 ggcgccgccg cagatttcgc agggagacgc cgcctggctc ggaaaccatc gtcccagtcc
     2761 ccagcaggcc gcgaggagca cggcgatccc gatggttgcc gcgggggaca aatgccgggc
     2821 gactttcgtc accgagtcct ggatgaccgg tgacgcgggt ggcgagcgaa gggcgtcggt
     2881 caagagctct tccgtggtgg tcgggtaatc gaccaccgcg aagtccgccc ctcgggcgga
     2941 cagttcgccg taccggtcga gcagtccggc gttgatgacg cgcgccgcct ggtacttgac
     3001 ctccccttca tcgaacttca ggtcggcccg atacgcttgg ctcaggttgt agagcaccgg
     3061 gaccgcgttc ggcaccacgg ccaaggcctg ctcgtaggcg gagatcgcgt gaccgtagtc
     3121 ctgacggcgg aacaggatgt tgccgtaccc ggtgagcaat cgcgggtcat tgggccaccg
     3181 gaccagggcc tcttgagaca acgtcacggc ctcatcggtt cgtccggccc gcatcaaggc
     3241 cgtcgcccga gccacggcgc cgtcggccga gtcgacgatt tcggagtccg ccagcgccac
     3301 ggcggggtct cggccttccg ccacgtccac gaccaaccgg aactccgcgt tttcgtccgc
     3361 ggtcaggatg ggcacgaccc gaggaatcag cagtgacgcg agaatgccga gggcggccaa
     3421 cccgcccagc accacgcgtt cgcgacggga cagaaaccac cacaacgccg cacccggaac
     3481 catgacgtac caggcgctcc attgaaccat cgcaagcggc gcgatcaaca accatagtgc
     3541 agcgagccag gcggttggtc gaaagatccg gtgtccgctc cattcgacga atagatgggc
     3601 gaggagcggg atgacccgga tcaccagcac caggatgacc acgaccaccg ccgataacag
     3661 gctcaggacg gccgccgcca cccatcggtc gatccggtac aacagcgacc aaaagtctcg
     3721 gtaggatgcg ttgagcgccg ccacccattc cttcccgctg gccagcgcgt cggggctggg
     3781 gccggagaga agagccgagg ccagccagat gtgcggggac gacaattcag gagccagcga
     3841 cgaggcgatt tgcgcgagtt cgatggcctc ctcgcgcgct ccccgtttga gcaaccggtc
     3901 cgcgtcgcgg acgatcacca gactccacgc ggggtattgc tggatgtgtc cgttccgcgc
     3961 ggcgtccgcg acggcgcgcg ccgcgaccgg aatttccccg gattccgcgg ccgagtcgac
     4021 cgtcgcccga taggcgttcc atgccgcttg cggatcaaca tccgtcgcgg cgatcacggg
     4081 cgagacgaat gagagggtga ccaccagggt acggaccgtg gccgcccaca ggtgcatcag
     4141 acgcggcgtc ccaccggtga ttcggtcccg gccagaaggc gccgaatatt ttcctgatgt
     4201 cggatgacga tcacggcggc cacgaccacg gaaaacaccc ggagccgaga cggtcccagc
     4261 gcgaagaccg caatcgggag cgaggccgcg gccgtgatgc tggccagcga cacatagcga
     4321 aagagcgcca acacggtgat ccaggccagg agcactcccc ccgcggttat cggcgccaac
     4381 gccgccagcg cgccgatgcc ggtggccacg ccctttccgc ctcgaaaccc cgtccacacc
     4441 ggaaacgtgt gacccgcgac aaccgccatg ccggaaatct ctgcccaccg cgccgtctga
     4501 tccgccgtca gcaacgacgc cgcccacacc gcgacggccc ccttgcccgc atccagtaac
     4561 aatgtagcgg ccgcaggccc cttgcccgcg acccgtagga cgttcgtgct cccgatgttt
     4621 ccgcttccct tggtcctggg atccgaggcg ccgatccacc gggcgaccac cacgccaaaa
     4681 ggaatcgtgc caatcatata cgcgaacagc acaatcgcca agccgaccag gagggacgaa
     4741 tccgcggtca tgtcgcggaa gtcgggccga gcgagcggcg ataggcc
//
LOCUS       DBNGTT010000396         1852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_605821, whole genome shotgun sequence.
ACCESSION   DBNGTT010000396 DBNGTT010000000
VERSION     DBNGTT010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_605821"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC16BC_09000"
     CDS             <1..474
                     /locus_tag="AC16BC_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR3241796.1"
                     /translation="LFALPRLLAGPVTGVLVDAIGWRDFFLVTIVVGVPGLVMLQRFC
                     PVGTREPLLTADRASQGPPVPARSLVFRGVVGALGGFLGGTVTVAALAGLKAIHKTPD
                     APFALGSRVAELVTPATVGGWAQLVGLVLFGLVVGLAVAATAAARSRVARGGDVR"
     gene            621..>1852
                     /locus_tag="AC16BC_09005"
     CDS             621..>1852
                     /locus_tag="AC16BC_09005"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3241797.1"
                     /translation="MSVTATSTPSLAALLRHRAAVLRMDSVRATSEAGSGHPSSCCSA
                     ADIVATLFFSVMRYDPEDPRHPGSDRFVLSKGHAAPLLYAAWAEAGLIPKADLLKLRT
                     LTSDLEGHPTPRLSFVDMATGSLGQGLSAGVGMAVAAKRLQRSAARVYVLMGDGESVE
                     GSVWEAAEIARHERLDNLCAIVDVNRLGQSDPTMLQHDMEAYRARWSGFGWNAVVVDG
                     HDVEALVAAFGAAAATNGKPSVVLARTFKGKGISFVEDRPDWHGKALKKGEELDRALA
                     ELSKGLDPNATAPVITRPSPPAAIATTPKLVAPPSYKPTDVVATREAFGHALAAVGEA
                     NPLVVGLDADVKNSTFTDAFGKRFPERFLENYIAEQNMVGAAVGLAASGYIPFAATFA
                     CFLTRAYDFIRMAAISGA"
BASE COUNT          282 a          610 c          631 g          329 t
ORIGIN      
        1 ctctttgcgc tgccgcgtct gctcgcgggt ccggtcaccg gggtcctcgt ggatgcgatc
       61 ggttggcggg acttcttcct ggtgacgatc gtggtcggcg tgcccggcct cgtgatgctc
      121 cagcggttct gcccggtggg tactcgcgaa ccgttactga cggcggaccg cgcatcccag
      181 ggcccgcccg tgccggctcg ctccctcgtc ttccggggcg tggtcggcgc gctgggcgga
      241 ttcctcggcg ggacggtgac cgtcgcggcg ctggcgggtt tgaaagccat ccacaagacc
      301 ccggacgcac cgtttgccct cgggtcgcgc gtcgcggagc tggtcacgcc cgcgacggtt
      361 ggcgggtggg cccaactggt cggcctggtc ttgttcggcc ttgtggtcgg cctcgccgtc
      421 gccgccaccg cggccgcgcg gtcacgggtt gcgcgaggcg gcgacgtgcg ctgatcgccg
      481 cgtcaaaaca cgcgtgctcg aactgccgag agacgtcgat ctgaaattga ctcgtccgaa
      541 acgattttga taccctctcg ccgtcctcgc gccgatctgc cggctcgcgt accaccagac
      601 atgcacacaa aggagattcc atgagcgtca ccgctacttc gaccccgtcg ttggccgcgc
      661 tgttgcgcca tcgagccgcc gtcctgcgga tggacagcgt gcgcgccacc agtgaagccg
      721 gcagcggcca cccgtcgagt tgttgctcag cggccgacat cgtggccacc ttgttctttt
      781 ccgtgatgcg ctacgacccc gaggatccgc gccatcccgg aagcgatcgc ttcgtactgt
      841 caaagggcca cgcggcgccg ctgctgtacg cggcatgggc cgaagccgga ctgataccca
      901 aagccgatct gttgaagttg aggacgctga cgtctgatct ggaaggccac ccgacgccgc
      961 gcctctcgtt cgtggacatg gccacgggct cgttggggca agggctctcg gccggcgtgg
     1021 gcatggcggt ggccgcgaag cgtttgcaac gatcggccgc gcgcgtgtac gtgctgatgg
     1081 gggacgggga atcggtcgaa ggctcggtgt gggaggccgc cgagatcgcc cgtcacgagc
     1141 gcctcgacaa cctctgcgcg atcgtggacg tcaaccgcct gggacagagc gatcccacca
     1201 tgctccagca cgatatggag gcctatcgcg cccgatggtc ggggttcgga tggaacgccg
     1261 tcgtcgtgga tgggcacgac gtggaggcgt tggtggcggc gttcggcgcg gccgccgcca
     1321 cgaacggaaa accctcggtc gtcttggcgc gtacgtttaa aggcaagggg atctcgttcg
     1381 ttgaagaccg tcccgactgg cacggaaagg ccctcaagaa gggcgaggag ctggaccgcg
     1441 cgctggcgga attgtccaag ggactcgacc ccaacgcgac cgctccggtg atcacacgtc
     1501 cgtcgccccc ggccgcgatc gcgacgacgc ccaaactcgt cgcgcccccg tcgtataagc
     1561 cgaccgacgt ggtggcgacg cgcgaagcgt ttgggcacgc gttggcggcc gttggcgaag
     1621 ccaatcccct ggttgtggga ttggacgcgg acgtgaaaaa ttcgacgttc accgacgcct
     1681 tcggcaaacg gttcccggag cggttcctcg aaaactacat cgccgaacag aacatggtgg
     1741 gggcggcggt cggactcgcc gcgagcggat acatcccatt tgccgccacc tttgcctgtt
     1801 tcctcacccg cgcgtacgat tttatccgca tggccgcgat cagcggcgcg aa
//
LOCUS       DBNGTT010000397         3256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2649555, whole genome shotgun sequence.
ACCESSION   DBNGTT010000397 DBNGTT010000000
VERSION     DBNGTT010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2649555"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="AC16BC_09010"
     CDS             complement(<1..455)
                     /locus_tag="AC16BC_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3241798.1"
                     /translation="MSKIEVTTILVPTDFSDGSLQAMRYAMGLSELLGARVVFLHVVE
                     PPAYGVELTGTYPGIAPDVTKKLAAMMHDWVEQVMVRGARVEWHLEMGSAFAEILAAV
                     ERYGADLIVMGTHARTGLSHLLLGSVAARVVQHAPCPVLTVRASAAAPAP"
     gene            complement(676..1146)
                     /locus_tag="AC16BC_09015"
     CDS             complement(676..1146)
                     /locus_tag="AC16BC_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3241799.1"
                     /translation="MIPIKRILLATDFSDCSRAAIDYALAFAERLGAEVHLVHVFERP
                     VYFEVGVSHRLQLGRDVDEWIRDLKADATKQLDALAAEVRGRKPGTQVSMREGLPVDE
                     ILLAAKALPADVIVLGTHGRTGLPHVLLGSVAERVVRGAPCPVLTVRLPVGARP"
     gene            complement(1317..>3256)
                     /locus_tag="AC16BC_09020"
     CDS             complement(1317..>3256)
                     /locus_tag="AC16BC_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3241800.1"
                     /translation="VFALGVWRGEPFTLMLLTAVSLAVAVIPEGLPAMVAIVLALGAL
                     RMARRQALIRKLPAVESLGCVTTVCSDKTGTLTQNVMSVALISQGGRMVEVTGEAYRP
                     EGAFIEGGKRIDPRTDEALARLLRSAALCTNARLQLHEDHWTVLGDPTEGALLAAAAK
                     AGLWKETLEGEYPRLAELPFDSDRKLMSAVHQDRAGRVWVVTKGGIDEVLARSTRVSW
                     DGAEEPLTRSRREQIARINRELSSDGVRVIACAVRSLDALPHAEDLAGVEDDLCFLGL
                     FGMMDPPRPEVKAAVVRCREAGIRPVMITGDHRVTAEAIAVNLGIKTPADTVLTGEEV
                     ERMDPDALQREAGRTAVYARVSPSHKVKIVTALKRRGEVVAMTGDGVNDAPALKTADI
                     GVAMGRGGTHVAREAAHMVLLDDNFSTIVAAVEEGRIIYANIRKFTRYMLSTNSGEIL
                     TVMLAMLVGLPIPLLPVQILWLNLVSDGLPALALGFEPPERSVMSRPPRRPDETIFAD
                     GMGAHIAWVGLLMGVGTAAVFAWALAQGDLARAHTMAFFTLTAFQMFHVLAIRSEREA
                     LWTIGLWSNPRLLAAVALAVAAQLALTYHPALQEFFHTTALTGRELAVCGAVASSVYV
                     AVEVEKWWRRARDRSGMISHG"
BASE COUNT          549 a         1198 c         1018 g          491 t
ORIGIN      
        1 ggggccggcg cggccgcgct cgcccggacc gtcaacaccg gacacggcgc gtgctgcacc
       61 acccgggccg caacgctccc caacaacaga tgcgacagac cggtgcgggc gtgggtgccc
      121 atcacgatga gatccgcgcc gtaacgctcg accgcggcga ggatctccgc aaacgccgag
      181 cccatctcca ggtgccactc cacgcgcgcg ccgcgcacca tcacctgttc gacccagtcg
      241 tgcatcatcg cggccagttt tttggtcacg tcgggcgcga tgcccggata ggtcccggtc
      301 aattccaccc cgtacgcggg cggctccacc acgtgcagaa acaccacgcg ggcgccgagc
      361 agctccgaca gccccatggc gtaccgcatc gcctgcagcg agccgtcgga aaaatccgtg
      421 ggcaccagaa tagtcgtgac ctcgatcttg ctcatcgcac tctcctcgac tgtctcagaa
      481 cttgtctgaa ttcactgcga agcagagccc gttcagggaa ggccagatgc caggcgaacc
      541 cgccttgcgc cgacggcgca agatgcaatc gacgtcgtcc tctcgggtgg cgaagcaagg
      601 gaggaccttg cgcgcggagc gtaccgggtc gtacgtgagc acgcaaggcc gaccgacaac
      661 gccgcagatg gccgttcatg gacgggctcc taccgggagc cggaccgtca acaccggaca
      721 cggcgcaccc cgtactacgc gctcggccac gctgcccagc agcacgtgcg gcaacccggt
      781 ccgtccgtgc gtgccgagca cgatcacgtc cgccgggagt gctttcgccg ccaggaggat
      841 ctcatcgacc ggcaatccct cgcgcatgga cacctgcgtg cccggcttgc gaccgcgcac
      901 ctcagcggcc agcgcgtcca gttgtttggt cgcgtcggct ttaaggtcgc gaatccattc
      961 atccacgtcg cgccccagtt ggagcctgtg agagaccccc acctcgaaat acaccggccg
     1021 ctcgaacacg tggaccagat gcacctccgc gcccaaccgt tcggcgaacg cgagcgcgta
     1081 gtcgatggcc gcgcgggagc agtcggaaaa gtcggtggct aagagtatgc gtttaatcgg
     1141 tatcattcaa tctcctgtgg gagcccgccc atgaacggcc atctgcggcg ttgccgctgc
     1201 gcatccgctc ctcacgtact caccacgtac gctgcggtgc ggtgctcgcg gcgcctggca
     1261 tcgggccgat tcctgaccgg gctgaatgtc aacttccgtc aacatgatcc gagcgtctag
     1321 ccgtgtgaga tcatgccact gcgatcgcgt gcccgcctcc accacttctc cacttccacc
     1381 gccacgtaca ccgacgacgc caccgcgccg cagaccgcca gttcccgccc cgtgagcgcg
     1441 gtggtgtgga aaaattcttg cagcgccgga tggtacgtga gcgccaactg ggcggccacg
     1501 gccagcgcga ccgcggccag caatcgcggg ttggaccaca ggccgatggt ccacaacgcc
     1561 tcgcgctccg accgaatggc gagcacgtgg aacatctgaa acgcggtgag cgtgaaaaac
     1621 gccatggtgt gggcgcgcgc caggtcgccc tgcgccaggg cccaggcgaa cacggccgcg
     1681 gtgccgacgc ccatcaacaa ccccacccac gcgatgtgcg cgcccatgcc gtcggcgaag
     1741 atggtttcgt cgggtcgccg cgggggccgg ctcatcaccg accgctcggg cggctcgaac
     1801 cccagcgcca acgccggaag tccgtccgaa accaggttca gccacaaaat ctgcacgggc
     1861 agcagcggaa tcgggagccc gaccagcatg gccagcatca cggtcaggat ctccccgcta
     1921 ttcgtcgaga gcatgtaccg cgtgaacttg cggatattag catagatgat gcgtccctct
     1981 tcaaccgccg ccacgatcgt cgagaaatta tcgtccagca gcaccatgtg cgcggcctcc
     2041 cgggccacgt gggtgccgcc gcggcccatg gcgaccccga tgtccgcggt cttgagcgcg
     2101 ggggcgtcgt tcacgccgtc gccggtcatg gccaccacct cgccgcgccg cttgagcgcg
     2161 gtcacgatct tgaccttgtg cgagggcgag acccgggcgt agaccgcggt gcgcccggct
     2221 tcgcgctgca gcgcgtccgg gtccatccgc tccacctctt cgccggtcag caccgtgtcc
     2281 gcgggcgtct tgatccccag attcacggca atggcttcgg ccgtgacccg gtggtcgccg
     2341 gtgatcatca ccggccggat gcccgcctcg cggcaccgca ccaccgcggc tttgacctcg
     2401 ggccgcggcg ggtccatcat cccgaacaac cccagaaagc acaggtcgtc ctccacgccg
     2461 gccaggtcct cggcgtgcgg caacgcgtcg agcgatcgga cggcgcacgc gatcacgcgc
     2521 acgccgtcgg acgacaactc gcggttgatc cgcgcgatct gttcgcgccg cgaccgcgtg
     2581 agcggctctt cggccccgtc ccacgacacg cgggtggacc gggccagcac ctcgtcgatc
     2641 ccgcccttgg tcaccaccca cacgcggccc gcgcggtcct ggtggacggc gctcatcaac
     2701 ttccggtccg agtcgaacgg cagttcggcc aggcgcggat attcgccctc cagcgtctcc
     2761 ttccacaacc cggccttggc cgcggccgcg agcagggcgc cctcggtggg gtcgccgagc
     2821 acggtccagt gatcctcgtg cagttgcagc cgcgcgttgg tgcagagcgc cgcggagcgc
     2881 aacaaccgcg ccagggcctc gtccgtccgg ggatcgatcc gttttccgcc ttcgatgaac
     2941 gcgccttccg gccggtacgc ctccccggtc acctccacca tgcgcccgcc ctgcgagatc
     3001 agggccacgg acatcacgtt ctgggtgagg gtgccggtct tgtccgagca gacggtggtc
     3061 acgcacccga ggctttcaac ggccgggagt ttgcggatga gcgcttgccg tcgcgccatg
     3121 cgcaacgcgc ccagggccag gacgatcgcc accatggcgg gcaagccctc ggggatgacg
     3181 gccacggcca ggctcaccgc ggtcagcagc atgagcgtga agggttctcc ccgccacacc
     3241 cccagcgcga acacga
//
LOCUS       DBNGTT010000398         5269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2649631, whole genome shotgun sequence.
ACCESSION   DBNGTT010000398 DBNGTT010000000
VERSION     DBNGTT010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2649631"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..869)
                     /locus_tag="AC16BC_09025"
     CDS             complement(225..869)
                     /locus_tag="AC16BC_09025"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR3241801.1"
                     /translation="MFTGIVEELGVVQALERGLQSAVLTVLARRVIKGMNVGDSITVN
                     GVCLTVVRVTDQGFAVDVGPETLRVTTLGGLAPGDGVNLERAMRVGDRLGGHLVSGHV
                     EAVGRVASRTPDGNAINLTIEAPREIMRYCVGKGSITIDGVSLTITSLTEKTFGVMII
                     PHTAEVTNLGLKPPGSLVNLEPDLIAKYVERLLMGGDAKAEKVDAEFLRKRGLL"
     gene            complement(1025..1840)
                     /gene="thiD"
                     /locus_tag="AC16BC_09030"
     CDS             complement(1025..1840)
                     /gene="thiD"
                     /locus_tag="AC16BC_09030"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR3241802.1"
                     /translation="MGMPKIRTLPQALTIATSDSGGGAGIQGDIKALAAHGVFGLSVV
                     VAITAQNTTTVSRIHAVPSDVITAQLDAVFGDFAITAVKTGMLHSAEIVRTVADYLRT
                     RGPIRLVVDPVMVSSTGVRLLEPAAVKAMINDLLPLAEVVTPNVPEAEALTGMRIATI
                     DDARAAAQKIRQYGCRAVLIKGGHLSQAPATDVLFDGASWTEIPGEWIDSPHTHGTGC
                     AYSAAIAAHLATGADLVSAVRAAKTYITGAIRRGLAIGHGKGPMNLLYRLEEA"
     gene            1935..2720
                     /locus_tag="AC16BC_09035"
     CDS             1935..2720
                     /locus_tag="AC16BC_09035"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR3241803.1"
                     /translation="MDTWRLIMDPPSPAGYNMAVDEALADACRRGLIGPTLRLYEWDR
                     PSISLGYFQRPEAIVDLERCRDAGVPVVRRTTGGRAVYHHREVTYSIVAPTPHPLFAP
                     TIRGAYEVVARALASGLTDLGLRVELHPRDPGRPRRGARTPLCFDSTSRSELTLRGRK
                     VVGSAQRRWPAAVLQHGSILLAYDSSEAESWFLGHAAAAESITGLDAGRRDISPDDVR
                     RALCSGVERTFGVRLLLDTLSPDEADAVAQSSQGRDLTVTQVV"
     gene            2731..2880
                     /locus_tag="AC16BC_09040"
     CDS             2731..2880
                     /locus_tag="AC16BC_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241804.1"
                     /translation="MGTSALVLIVEDDPKTSNLLKVHLEREGYQTRSADGPQVVESRS
                     GTRPR"
     gene            2877..3044
                     /locus_tag="AC16BC_09045"
     CDS             2877..3044
                     /locus_tag="AC16BC_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3241805.1"
                     /translation="MIVLNLTRDSVHVLDRTVDVHIGRLRDKIEPTPSQPTYVLTVRG
                     LGYKFRDEDDA"
     gene            complement(3157..3669)
                     /locus_tag="AC16BC_09050"
     CDS             complement(3157..3669)
                     /locus_tag="AC16BC_09050"
                     /inference="COORDINATES: protein motif:HMM:NF018984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HIR3241806.1"
                     /translation="MTWVGPRLRDLHAITNTIGRGEPEAQRSLEEVRRFRGALRGTVE
                     RITQGKPVPRHVIEAINQRLRARTGYIEMTRVRGGFTKRVHAEFKAPPDLLVPMAEAA
                     SDLLCDADFSLIRRCENRQCVLFFYDQTKNHSRRWCSMQWCGNRMKVAAFYHRRREQG
                     TKARSTGRSG"
     gene            3774..4445
                     /locus_tag="AC16BC_09055"
     CDS             3774..4445
                     /locus_tag="AC16BC_09055"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HIR3241807.1"
                     /translation="MNTRWLAALTTGLTLTSGALTTVPAFADDEDSRRYAVTITNLTH
                     GQLLAPPVVIVHSQEFTLFTPGEPARPELAWLAEDGMTQPLVDLLATEQGVYQTHVGG
                     FVPPGESTTVEVEISGPFRLLTAAGMLATTNDGFYAIEDIKVSEKGGMEVDADAYDAG
                     SEANNESCAFIPGPPCGHPGVRDTVGAEGFVHIHAGIHGLADLVPATHDWRNPVAQVT
                     IERIR"
     gene            4680..>5269
                     /locus_tag="AC16BC_09060"
     CDS             4680..>5269
                     /locus_tag="AC16BC_09060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3241808.1"
                     /translation="MLLDPAGPNWISTDERGQRILDLFDGARTFGQVVHAYAAEAKLD
                     VVAAWQHVDTLARDAIRRGMLADQPIVHQPYRGRASYLGPRRPTELWLHTNNSCNLAC
                     SHCLVSSGQDGDKGLSTDVLLRVIRDARTLGVRRFFFTGGEPFLRKDITTLIDAALED
                     PKAELAILTNGILFTPTRIEELRQRDPARLKIQISL"
BASE COUNT          912 a         1849 c         1595 g          913 t
ORIGIN      
        1 gtcgggcgtc ggacacttcc cgccaggcgt catcgaacag atcctcgcgc acaacgcgcg
       61 cggcagcttc cggctctacg ccgaccctaa cggccgtctc aatgacgtgg ggtttcgctg
      121 cgcgaagtga accggagaac ccgggaatcg gagaaccggc gaaaaagatt tggctgttag
      181 ccttttcgcc ggttctccgg ctctcccatt ctccggttca atcatcacaa cagtccccgc
      241 ttccgcagga actccgcgtc caccttctcg gctttcgcgt ccccgcccat cagcaggcgt
      301 tcgacgtatt tggcaatgag atccggttcg agattgacga gcgaccccgg gggcttgagc
      361 cccaggttcg tgacttccgc ggtatggggg atgatcatca ccccgaacgt tttttcggtc
      421 aacgacgtga tcgtgagact cacgccgtcg atcgtgatcg agcccttgcc cacgcagtag
      481 cgcatgattt cgcgcggggc ctcgatcgtg agattgatgg cgttcccgtc cggggtgcgg
      541 gacgccacgc ggcccacggc ctccacgtgc ccgctcacca aatggccgcc caaacgatcg
      601 cccacccgca tcgcccgctc caggttcact ccgtctcccg gcgcaagccc gcccagcgtg
      661 gtcacgcgca gggtctcggg ccccacgtcc acggcaaaac cctggtcggt gacccgcacc
      721 acggtcagac acaccccgtt caccgtgatg ctgtcgccca cgttcatgcc cttgatcacg
      781 cgtcgcgcga gcacggtcag gaccgcggac tgcaacccgc gctccagcgc ctggaccacg
      841 cccagctctt ccacgattcc ggtaaacatc gcttggaatt cctcctaatt cagaacctac
      901 aacaagcccc ccctccctca ccctcccccg tcacggggga gggctacctg attggcccct
      961 ccccccttga gggggaggga agggtggggg gacgcattat catgccgtgg ggcgaatccg
     1021 cggttcatgc ctcctccaac cgatacaaga gattcatcgg cccttttccg tgaccgatgg
     1081 ccaagccgcg tcgaatcgcc ccggtgatgt atgttttcgc ggcccgcacc gcggacacca
     1141 ggtccgcgcc cgtggcaagg tgcgcggcga tggcggcgga gtaggcgcag cccgttccgt
     1201 gcgtgtgcgg gctgtcgatc cattccccgg gaatctcggt ccacgacgcg ccgtcgaaca
     1261 acacgtcggt cgccggcgcc tgcgacaagt gcccgccctt gatcagcacc gcacggcacc
     1321 cgtattgacg gattttttgg gccgcggcgc gcgcgtcgtc aatcgtcgcg atccgcatcc
     1381 cggtcaatgc ctcggcttcg ggcacattgg gcgtgaccac ctcggccaat ggcaacaaat
     1441 cgttgatcat cgccttcacc gccgcgggtt cgagcaaccg cacgccggtc gacgacacca
     1501 tcaccggatc caccaccaac cggatcggcc cgcgcgtgcg caagtagtcc gccacggtcc
     1561 gcacgatctc cgcggaatgc agcatgccgg tcttgaccgc ggtgatcgcg aaatcgccga
     1621 ataccgcgtc gagctgcgcg gtgatcacgt cgctcggcac cgcgtgaatc cggctgaccg
     1681 tggtcgtatt ctgcgccgtg atcgcgacca ccacggacag gccgaacacg ccgtgcgccg
     1741 ccagggcctt gatgtcgcct tgaataccgg cgccgccgcc ggaatcagac gtggcgatgg
     1801 tcagggcttg tggcagcgtg cgaatcttgg gcattcccat catgtcgtcc cacgtgaccg
     1861 cattctacat cctccccgtg ccgccttcaa cgcaggccct tgaccttggc ggccggaatc
     1921 ctcacactcg gcgcgtggac acgtggcgac tgatcatgga cccgccctcg cccgccggtt
     1981 acaacatggc ggtcgacgaa gccctggccg acgcgtgtcg gcgcggcctc atcgggccca
     2041 cgctgagatt atacgaatgg gatcgcccat cgatctcgct cgggtacttt cagcggcccg
     2101 aggcgatcgt ggatctggag cgatgccgcg acgccggcgt gcccgtggtc agacgcacca
     2161 ccgggggccg cgcggtctat caccatcgcg aggtcacgta cagcatcgtc gcgccgacac
     2221 cccacccctt gttcgctcca acgatccgcg gcgcgtatga agtcgtggcg cgcgcgctgg
     2281 cgtcgggatt gacggacctc ggattgcgcg tcgaactcca tccgcgcgac cccgggcgtc
     2341 cgcgccgcgg ggcgcggacg ccgttgtgtt tcgattcgac atctcgttcc gagttgacgt
     2401 tacgcggccg gaaggtcgta ggcagcgccc aacgccgatg gcccgccgcg gtcctccaac
     2461 acggctcgat cctgctggcg tacgactcat cggaagccga atcgtggttt ctcggtcacg
     2521 cggccgccgc cgaatccatc accggcctgg acgcggggcg ccgcgacatc tcgccggacg
     2581 acgttcgtcg cgccctgtgc tccggggtcg aacgaacctt cggcgttcgg ttgctcctgg
     2641 acacgctgtc gcccgatgag gcggacgccg tcgcacaatc gtcacaaggc cgtgacctca
     2701 ccgtgaccca ggtcgtgtaa catcactcgc atgggcacga gcgctctcgt tctgatcgtt
     2761 gaagacgacc ccaagacatc gaacctgctc aaggtccacc tcgaacgcga aggttatcag
     2821 acccgctccg ccgacggccc gcaggtggtc gagtcgcgca gcggcactcg ccctcgctga
     2881 tcgttctcaa cctcacgcga gacagcgttc acgtgctcga ccgcaccgtg gacgtgcaca
     2941 tcggccggct gcgcgacaaa attgagccca cgccctcgca gcccacctac gtgctcaccg
     3001 tgcgcgggtt gggatacaag ttccgcgacg aggacgacgc atgagcgtcg acgccgcgca
     3061 tcgccggcga ttgcttcatg cagttgatga aggaataccg catcgacccg gtcacgccgc
     3121 agcgaaccgc ggcgaattgg tcacccaaga gacggatcaa cccgatcgtc ccgtggaccg
     3181 ggcctttgtc ccctgttctc gccggcggtg gtaaaaggcc gcgaccttca tccggtttcc
     3241 gcaccattgc atgctgcacc accgccgact gtggttcttg gtctggtcgt aaaagaacag
     3301 gacgcactga cggttctcgc accgtctgat caaggagaag tcggcgtcac acagcagatc
     3361 gctcgccgcc tccgccatcg ggaccagcag gtccggcggc gccttgaatt ccgcgtgcac
     3421 ccgctttgtg aatcctccgc ggactctcgt catctcgatg tacccggttc gggcccggag
     3481 ccgttgattg atggcctcga tgacatgacg cggaaccggt tttccctgag tgatccgttc
     3541 gaccgttccg cgcaacgcgc cgcgaaaccg ccgcacctct tcaagcgacc gctgggcctc
     3601 cggctcgcct cgcccgatgg tattggtaat ggcgtgcaga tctcgaagcc gaggtcccac
     3661 ccacgtcatc gaatcgtcat acagcagtca tctgacctac acgtcgaggg cgtacggtgg
     3721 acgagtctcg ggcaggcggt ctgccccaca acccttggac aggaggacgg atgatgaaca
     3781 cacgatggct cgctgcactg acaaccggcc tgacgctgac gtccggcgcc cttaccacgg
     3841 tcccggcgtt cgcggatgac gaggacagcc ggcgttacgc tgtgacgatc acgaatctca
     3901 cccacggcca gctgctcgcg ccgccggtcg tcatcgtgca cagccaagag tttacgttgt
     3961 tcacgccagg cgaacccgcg cggccagagc tggcctggtt ggcggaggac gggatgaccc
     4021 aaccgctggt tgacctgctc gccaccgaac agggcgtgta ccagacacac gtaggcggct
     4081 tcgtcccacc tggcgagtcc accaccgttg aagtcgaaat ctccggcccg ttccggctgc
     4141 tgaccgccgc gggcatgctc gcgaccacca acgacgggtt ctatgcgatc gaggacatca
     4201 aggtgtccga gaagggcggc atggaagttg acgcggacgc gtacgacgcg ggcagcgaag
     4261 ccaacaacga gtcgtgcgcc ttcatccccg ggccgccgtg cggccacccc ggcgtccgcg
     4321 acacggtcgg ggccgagggc ttcgtgcaca ttcacgcggg cattcatggt ctcgccgacc
     4381 tcgtgcccgc gacgcacgac tggcgaaatc ccgtggcaca ggttacgatc gaacggattc
     4441 gatgatggga attcgtgcat gcgccggcgg aaccatcacg cgttgacatg cgccagcatg
     4501 cacggtagat tcgccctcgg ctcgttctgc gaggttcaca tcaatcggcg gccgggacca
     4561 catagggtcc cggccgcttg acattcctga ccatgacgta gggaggcagc atgctactga
     4621 cgcacgagac ggtactccac gctccgctcc gagagacgct ttcctccaac gggatccgcg
     4681 tgttgctaga tccggcgggg ccgaactgga tcagcaccga cgagcgcggc cagcgcattc
     4741 tggacctctt cgacggcgcg cgcacgttcg gccaagtggt tcacgcctac gcggcggagg
     4801 ccaagctcga cgtggtcgcg gcctggcagc acgtcgatac gctcgcgcgc gacgcaatcc
     4861 gccgcggcat gttggccgac cagccgatcg tgcaccagcc ctaccgtggc cgcgcctcgt
     4921 acctggggcc tcgccgcccg accgagctgt ggctgcacac caacaactcg tgcaacctgg
     4981 cgtgcagcca ctgcctggta tcctcaggtc aggacggcga caagggcttg tccaccgacg
     5041 tgctgctgcg cgtgatccgc gacgcccgga cgctgggcgt gcgccggttc ttcttcacgg
     5101 gcggcgagcc gttcctcaga aaagacatca ccacgctcat cgatgccgcg ctggaggatc
     5161 ccaaggcgga gctggcgatt ctaacgaacg gaattctgtt cactccgact cggatcgagg
     5221 agctgcgcca gcgcgacccc gcgcgcctca agattcagat cagcctgga
//
LOCUS       DBNGTT010000399         1842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1372990, whole genome shotgun sequence.
ACCESSION   DBNGTT010000399 DBNGTT010000000
VERSION     DBNGTT010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1372990"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="AC16BC_09065"
     CDS             complement(<1..639)
                     /locus_tag="AC16BC_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3241809.1"
                     /translation="MEREILQYLEPYDPGVFPAAVTARYGIPESRIANLASNETPYRL
                     PRAVITGLCRSVGEVTRYPDPSYRRAKAAVAAYVRCSPDAVSVGNGASEVLATLAMVT
                     LESHARVVIPIPSYSLYLFVAMAQNASLDLVQMDPPGFAFDADRVIERARDAALVLIC
                     SPNNPTGASVPASAVTRLHEETSAVIAVDEAYAEFSGRSVLSLLKTHPRLVVI"
     gene            complement(658..966)
                     /locus_tag="AC16BC_09070"
     CDS             complement(658..966)
                     /locus_tag="AC16BC_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241810.1"
                     /translation="MGVHRITSPAAKAYMQRERILGEGLSLLAAAAEKPTVSPQALEA
                     AGDLAAQLVPHAPGYGGKALQIVATQFWRQAKVKEKAAEMLSFEDLEKKVNALARLIG
                     "
     gene            complement(1008..>1842)
                     /gene="glnA"
                     /locus_tag="AC16BC_09075"
     CDS             complement(1008..>1842)
                     /gene="glnA"
                     /locus_tag="AC16BC_09075"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3241811.1"
                     /translation="EMTMELQRMGIPVERQHHEVATAGQAEIDIRFDSLVNMGDKLMT
                     FKYVIKNVAKRYGKSVTFMPKPLFGDNGSGMHTHQSIWKDGKPLFAGKEYAGVSQMCL
                     HYIGGILKHAGAIAALTNPTTNSYKRLTPGFEAPVNLAYSSRNRSASIRIPMYSSSPK
                     AKRIEVRFPDPSCNPYLAFSAMLMAGLDGIENKIDPGPAVEGDLYEMSPKELSRIKTM
                     PGSLDEALENLSVDHAFLLKGGVFTKDVIETWIGYKREKEVDAMRLRPHPYEFFLYYD
                     I"
BASE COUNT          321 a          600 c          577 g          344 t
ORIGIN      
        1 aatcacgacc agccgcggat gggttttgag cagggacagc acgctgcggc cggagaactc
       61 ggcgtacgct tcatccacgg caatgaccgc ggaggtctcc tcgtgcaacc gggtgacggc
      121 ggaggccggc acggacgcgc cggtcggatt gttgggcgaa cagatcaaca ccagggcggc
      181 gtcgcgcgcg cgctcgatca cccgatccgc gtcgaacgcg aatcccggag ggtccatctg
      241 caccaggtcg agcgaggcgt tctgcgccat ggcgacgaac agatacaggg aatacgacgg
      301 gatcggaatg accacacggg cgtgcgactc gagcgtgacc atggccaacg tcgcgagcac
      361 ctcgctcgcc ccgttgccca cgctcaccgc gtcgggcgaa caccgcacgt acgccgcgac
      421 cgcggccttg gctcggcggt aggacggatc ggggtaccgc gtcacctccc ccacactccg
      481 acacaacccg gtgatcaccg cgcgcgggag acgatacggc gtctcgttcg acgcgaggtt
      541 cgcgatccgg gactcgggaa tcccgtaccg cgcggtgacg gccgcgggaa aaactccggg
      601 gtcgtatggt tcgagatatt ggagaatctc ccgttccatg acgaccccgg aaagccctta
      661 accgatcaga cgagcgagcg cgttgacctt cttctccagg tcctcgaacg acagcatttc
      721 cgccgctttc tccttgacct tggcctgccg ccagaactgg gtggcgacga tctgcagcgc
      781 cttgcccccg taaccgggtg cgtgcggcac cagctgcgcg gccaaatcgc cggccgcttc
      841 caacgcctgg ggactcacgg tcggtttctc ggccgcggcc gccaataacg acagcccctc
      901 tcccaggatt cgctcccgct gcatgtacgc cttggcagca ggacttgtaa tgcgatggac
      961 gcccatcagt ccctccttgg ttcacgatcc gagtggatgc tcggcgatta gatgtcgtag
     1021 tacaggaaga attcgtacgg atgcggacgc agtcgcatcg cgtcgacctc tttctcgcgt
     1081 ttatagccga tccacgtctc gatcacgtcc ttggtgaaca ccccgccctt gagcaggaac
     1141 gcgtggtcca ccgacaggtt ttccaacgct tcatccagac tgcccggcat ggtcttgatg
     1201 cgcgagagtt ctttcgggct catctcgtac agatcacctt cgaccgccgg gcccggatcg
     1261 atcttgttct cgatcccgtc gagtccggcc atcaacatcg cggagaacgc gaggtacggg
     1321 ttgcaggacg gatcagggaa ccgcacttcg atgcgcttgg ccttcgggct gctggagtac
     1381 atcgggatgc ggatcgacgc ggagcggttg cggctcgaat acgccaggtt caccggcgct
     1441 tcgaaccccg gggtcaggcg cttgtaggag ttcgtggtcg ggttggtcaa ggccgcgatc
     1501 gcgcccgcgt gcttgagaat gccgccgatg tagtgcaggc acatctgcga cacgccggcg
     1561 tactccttgc ccgcgaagag cggcttgccg tccttccaga tggactggtg cgtgtgcatg
     1621 cccgagccgt tgtcgccgaa gagcggcttg ggcatgaacg tgacgctctt gccgtagcgt
     1681 ttggccacgt tcttgatgac gtacttgaac gtcatgagct tgtcgcccat gttcacgagc
     1741 gaatcgaacc ggatgtcgat ctcggcctgc cccgcggtcg ccacctcgtg gtgctggcgc
     1801 tcgaccggga tgcccatccg ctgcaactcc atggtcatct cg
//
LOCUS       DBNGTT010000400         5823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3161379, whole genome shotgun sequence.
ACCESSION   DBNGTT010000400 DBNGTT010000000
VERSION     DBNGTT010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3161379"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /locus_tag="AC16BC_09080"
     CDS             <1..1966
                     /locus_tag="AC16BC_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR3241812.1"
                     /translation="GDPIPIEQVEPLAEIVKRFTTGAMSYGAISKEAHETLAVAMNRI
                     GAKSNTGEGGEDPERFNPLPNGDSKNSAIKQVASARFGVTIHYLAHAKELQIKMAQGA
                     KPGEGGQLPGHKVDETIARMRFATPGVQLISPPPHHDIYSIEDLAQLIFDLKNANPEA
                     AVSVKLVSEVGVGTVAAGVAKVHADKILISGDSGGTGASPLASIKYAGIPWEIGLAET
                     QQTLVKNNLRGRVRIETDGQLKTGRDVVIAALLGAEEFGFSTAPLIVEGCIMMRKCHL
                     NTCPVGVATQDPVLRAKFTGQPEHVVNYFFFVAQEVRELMAQLGFRTVAEMIGRVERL
                     KIHKAVDHWKAKGLDLTPLLSKPEVADSVPLRCVESQQHGIDDVLDRALIERAQAAIE
                     RREPVEIALPIRNVHRTVGAMLSNQIAKRYGDEGLPQDTIRIAFTGTAGQSFGAFLAR
                     GVTLTLEGESNDYLGKGLSGGRIIVYPPKGSTFVPEETILVGNTVLYGATSGEGFFYG
                     MAGERFAVRNSGARAVVEGVGDHGCEYMTGGVVVVLGRTGRNFAGGMSGGIAFVLNED
                     GKFEQRCNLGMVELEPVREAEDKALLKDFIQRHLDYTGSRKAKTVLDRWDDFLPKFVK
                     VVPTEYRKVLDARKVSAQKHSSQKPAPVHG"
     gene            1959..3407
                     /locus_tag="AC16BC_09085"
     CDS             1959..3407
                     /locus_tag="AC16BC_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR3241813.1"
                     /translation="MADIRGFLKVKREGPKRRPVEERVHDWKEFYQPISEDTLRNQGS
                     RCMDCGVPFCQGNTGCPVENLIPEWNDLVHRGRWRDALKALHNTNNFPEFTGRLCPAP
                     CESACVLGVIDDPVSIRVIEWNIIDKGFDEGWVSPILPVNETGRRVAVVGSGPAGRAA
                     AQQLRRAGHQVTVFEKADRIGGLLRYGIPDFKMEKWVIDRRLEQMTAEGVQFQTNVDV
                     GRDVSADRLRREYDAVLLATGAMVPRDLKVPGRELAGVHFAMDYLTQQNKINAGDPPP
                     SADQRITADGKRVVIIGGGDTGSDCLGTAHRQNVKEVYQFELLPEPPPQRDASTPWPY
                     WPMQLRSSHAHEEGCKREWSVSTTKLSGRNGRVEKLHAVRGEFEKDAQGKTKMIEVPN
                     SAFELNADLVLLAMGFTGPEKPGLLTDLGVPLDPRGNVSVDANYMSSVPGVFAAGDAK
                     RGASLIVWAIAEGRKAAAGIERFLAAKSPVGR"
     gene            3520..3888
                     /locus_tag="AC16BC_09090"
     CDS             3520..3888
                     /locus_tag="AC16BC_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HIR3241814.1"
                     /translation="MAVVVYGREGCPMCADAKQWLTERKIAFTFREIFEQPLSHQELL
                     ALAAKVPDGLHGLYAPKGARKVGLPEDGRALTPDQIISLQVSNPDLIRYPLFEIDNQL
                     LFGFHQATRNALTALAGESR"
     gene            3885..4280
                     /gene="msrB"
                     /locus_tag="AC16BC_09095"
     CDS             3885..4280
                     /gene="msrB"
                     /locus_tag="AC16BC_09095"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3241815.1"
                     /translation="MTEKIKKSDAEWKAQLTPEQYQVTRKKGTERAFTGEYHDSKEPG
                     TYTCVCCGNPLFSSETKFDSGTGWPSFYQPMSPDAVATEDDSSFFMRRTEVLCSRCDA
                     HLGHVFPDGPKPTGLRYCINSASLKKTKP"
     gene            4315..4713
                     /locus_tag="AC16BC_09100"
     CDS             4315..4713
                     /locus_tag="AC16BC_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3241816.1"
                     /translation="MEPRISFITLGVSDLERATRFYEHGLGLPRLETPPSVTFFEMGK
                     TWLALWPWENLAADAGLSPQSSGFPRFSLAHNVRSPAEVDALLAHVAKFGAKVVKPGQ
                     PTFWGGYAGYFTDPDGFLWEVAHNPDFPHV"
     gene            4824..5327
                     /locus_tag="AC16BC_09105"
     CDS             4824..5327
                     /locus_tag="AC16BC_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241817.1"
                     /translation="MHDTNNDFWIPDREIEVIHALNSTSTKYLVIGGYAVLYYGYSNR
                     QVDDLDIWVNNSQDNAERLISALQTLLQNNEPPSIERLVKPTPLKIDLRNSRYEVELL
                     TMVKGVSFENAYSDRKVSSQDGENIDIVSIGDLLTIKRIAAQESATRLHKELKDIGFL
                     ESRSEEQ"
     gene            complement(5616..5753)
                     /locus_tag="AC16BC_09110"
     CDS             complement(5616..5753)
                     /locus_tag="AC16BC_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241818.1"
                     /translation="MTDGTVLQPLKHVNRLLQIERHHLRAAGSDLGLGGYSRSVFRGP
                     A"
BASE COUNT         1176 a         1861 c         1752 g         1034 t
ORIGIN      
        1 cggcgacccc attcccatcg aacaggtcga accccttgcg gagatcgtga agcgcttcac
       61 caccggcgcc atgtcctacg gcgccatcag caaggaagcg cacgagaccc tcgccgtggc
      121 catgaaccgg atcggcgcca agagcaacac gggcgaaggc ggcgaggacc cggaacggtt
      181 caatcccttg cccaacggcg actccaagaa cagcgcgatc aagcaggtgg cgtccgcgcg
      241 attcggcgtg accatccatt acctggccca cgccaaggaa ttgcagatca agatggcgca
      301 gggcgccaag cccggcgagg gcggccagct ccccggccac aaggtggacg agaccatcgc
      361 gcgcatgcgg ttcgccacgc ccggcgtgca attgatctcg ccgcccccgc accacgacat
      421 ttacagcatc gaggatctcg cgcagttgat cttcgatctc aagaacgcga accccgaggc
      481 cgcggtgtcg gtcaagttgg tttccgaggt cggggtcggc acggttgccg ctggcgtggc
      541 caaagtgcac gcggacaaaa ttctgatcag cggcgactcc ggcggcaccg gcgcgtcccc
      601 gctcgcgtcc atcaagtacg cgggcatccc gtgggaaatc gggttggccg aaacccagca
      661 gaccctggtc aagaacaact tgcgcggccg cgtacgcatc gaaaccgacg gccagctcaa
      721 gaccggccgc gacgtggtga tcgccgcgtt gctcggggcc gaggagttcg ggttttccac
      781 cgcgcccttg atcgtcgaag gctgcatcat gatgcgcaag tgccacctca acacctgccc
      841 ggttggcgtg gccacccagg acccggtgct gcgcgccaag ttcacggggc aacccgaaca
      901 cgtggtgaat tacttcttct tcgtggccca ggaagtgcgc gagttgatgg cgcaactggg
      961 attccggacc gtcgcggaga tgatcggacg cgtggagcgg ctcaagatcc acaaggccgt
     1021 ggaccactgg aaagccaagg gtctggacct cacgcccctg ctgtccaagc ccgaggtcgc
     1081 ggactcggtg ccgctccgct gcgtggagtc ccaacaacac ggcatcgacg acgtgctgga
     1141 ccgcgcgctg atcgagcggg cccaagccgc gatcgagcgt cgcgaacccg tggagatcgc
     1201 cttgccgatc cgcaacgtgc accgcacggt cggcgccatg ctctccaatc agatcgccaa
     1261 gcggtacggg gacgaggggc tgccgcagga caccatccgc atcgcgttta ccggcaccgc
     1321 gggccaatcc ttcggcgcgt tcctggccag gggcgtcacg ctcaccctgg aaggcgagtc
     1381 caacgattac ctgggcaagg gcctgtccgg cggccgcatc atcgtgtatc cgcccaaggg
     1441 ctccacgttc gtgcccgagg aaaccattct ggtcggcaac accgtgctct acggcgccac
     1501 ctccggcgaa ggctttttct acggcatggc gggcgagcgc ttcgcggtcc gcaactcggg
     1561 cgcgcgcgcg gtggtcgagg gcgtgggcga ccacggctgc gagtacatga ccggcggcgt
     1621 ggtggtggta ctgggtcgca ccggccgcaa cttcgcgggc gggatgagcg gcggcatcgc
     1681 gttcgtgctc aacgaggacg gcaagttcga gcaacgctgc aacctcggca tggtcgagtt
     1741 ggaacccgtg cgcgaggccg aggacaaggc attgctcaag gatttcatcc agcggcacct
     1801 ggactacacg ggcagccgca aggccaagac cgtcctggat cgctgggacg acttcctgcc
     1861 caagttcgta aaggtggtcc ccaccgagta ccgcaaggtg ctcgacgcgc gcaaggtctc
     1921 cgcgcagaag cactcgtccc agaagccggc gccggtccat ggctgacatc cgcggttttc
     1981 tcaaggtcaa acgcgaaggg cccaaacgcc ggccggtgga agaacgcgtc cacgactgga
     2041 aagagttcta ccagccgatt tccgaggaca cgctccgcaa ccagggctcg cgctgcatgg
     2101 attgcggcgt gccgttttgc cagggcaaca cgggttgtcc ggtcgagaat ctgatcccgg
     2161 aatggaacga cctggtgcac cgcggccgct ggcgcgacgc gctcaaggcc ttgcacaaca
     2221 ccaacaactt cccggagttc accggccgcc tctgccccgc gccgtgcgaa tccgcctgcg
     2281 tgttgggcgt gatcgacgac ccggtgtcga tccgggtgat cgaatggaac atcatcgaca
     2341 agggttttga cgaaggctgg gtctccccga tcctgcccgt caacgaaacg ggccgccgcg
     2401 tggcggtggt gggctcgggc ccggccggcc gggccgcggc ccagcaactg cgccgcgcgg
     2461 ggcatcaggt cacggtcttc gagaaagccg accgcatcgg cgggctcctg cgctacggca
     2521 tcccggactt caagatggag aagtgggtca ttgaccgccg cctggagcag atgacggccg
     2581 agggcgtgca gttccagacc aacgtggacg tgggccgcga cgtgtccgcc gaccgattgc
     2641 gacgcgagta cgacgcggtc ctgctcgcca ccggcgccat ggtgccgcga gacctcaagg
     2701 tcccgggccg cgaactcgcg ggcgtccact ttgccatgga ctatctgacc cagcaaaaca
     2761 aaatcaacgc gggcgacccg ccgccgtccg cggatcaacg catcaccgcg gacggcaaac
     2821 gcgtcgtgat catcggcggc ggcgacaccg ggtccgactg cctgggcacc gcgcaccgcc
     2881 agaacgtcaa agaggtctac cagttcgaac tgctccccga accgccgccc cagcgcgacg
     2941 cctccactcc gtggccctac tggcccatgc aactgcgctc ctcgcacgcg catgaagaag
     3001 gctgcaagcg cgaatggagc gtctccacca ccaagctctc cggccgcaac ggccgcgtgg
     3061 agaaactgca cgccgtgcgc ggggaattcg aaaaagacgc gcagggcaag accaaaatga
     3121 tcgaggtccc caactccgcg ttcgagttga acgcggacct ggtcctgctc gccatgggat
     3181 ttaccggtcc cgaaaaaccc ggcctgctca ccgacctagg cgtaccgctc gatccccgcg
     3241 gcaacgtgtc ggtggacgcg aactacatga gcagcgtgcc cggcgtgttc gcggccggcg
     3301 acgccaaacg cggcgcctcg ctcatcgtct gggcgatcgc cgaaggccgc aaagccgctg
     3361 caggtattga gcgcttcctc gccgccaaat cgccagtcgg gcgctaacac ctatcccttt
     3421 catcgccgtt tctgaatccc ccctttggaa aaggggggct ggggggattt gccccttgac
     3481 ctaacgcgga tcaatgcgga atgatggcag gcacgaacta tggccgtcgt cgtttatggt
     3541 cgcgaaggct gtcccatgtg cgcggacgcg aagcagtggc tgaccgagcg caagatcgcg
     3601 ttcacgtttc gcgagatttt tgagcagccc ctgtcgcacc aggaactcct cgcgctcgcg
     3661 gccaaagtcc ctgacgggct tcacgggctg tacgcgccaa aaggcgcacg caaggtgggt
     3721 ttaccggagg acggtcgcgc gctcacgccc gaccagatca tcagcctcca ggtcagtaac
     3781 ccggacttga ttcgttatcc actgttcgag atcgacaacc aactgctgtt tggttttcac
     3841 caggccacgc gcaacgcgct cacggcattg gcaggagaat ccagatgacc gagaagatca
     3901 aaaagtccga cgcggaatgg aaagcccaac tcacgcccga gcagtatcag gtgacccgca
     3961 agaaaggcac cgaacgcgcg ttcacgggcg agtaccacga ctccaaggag cccggcacct
     4021 acacctgcgt gtgctgcggc aatccgctct tcagctccga aacgaagttc gactcaggca
     4081 ctggctggcc cagcttctac cagcccatgt cccccgacgc ggttgccacc gaggacgaca
     4141 gcagcttctt catgcgccgc accgaagtcc tctgcagccg ctgcgacgcg cacctgggcc
     4201 acgtgttccc agacggcccc aagcccacgg gcctgaggta ctgcatcaac tccgcgtcac
     4261 tgaagaaaac caaaccctga tcggaggcgc gccgtattgc gcgcgaagaa ggcgatggag
     4321 cctcgaatca gctttatcac gctcggcgtc tcggaccttg aacgggccac gcgcttctac
     4381 gagcacggcc ttggcctgcc gcgactcgaa acgccgccgt cggtgacgtt cttcgagatg
     4441 ggaaagacct ggctcgcact gtggccatgg gagaacctgg cggccgacgc cggcttgtcg
     4501 ccgcaaagct ccgggttccc cagattttcc ctggcccaca acgtccgctc cccggccgag
     4561 gtcgatgcac tgctggccca cgtggccaag ttcggcgcca aggtggtaaa gcccggccaa
     4621 cccacattct ggggcggcta cgccggctac ttcaccgacc ccgacggttt cttatgggag
     4681 gttgcgcaca acccggactt cccgcatgtg tagtgttgtg ttgaagtggt tctagatgat
     4741 gaccaaatcc atgttggcga gaatgcgacc cactctgggt caggcttgac ttcttgactc
     4801 tgttagaata tccagccgtc gctatgcatg atacgaataa cgatttctgg atcccggata
     4861 gagagatcga ggtgattcat gcattgaact ccacatcaac caaatatctc gtgataggag
     4921 gatatgcggt cctttattat ggctactcga accgccaagt agatgattta gatatctggg
     4981 tgaataattc ccaagataat gccgaaagat tgatctcagc cctgcagacc ctattgcaga
     5041 acaatgagcc accttccatt gagcgcttag tgaaaccgac acccctaaag atcgacttgc
     5101 gcaatagccg ttacgaggtg gagttgctga ctatggtcaa gggtgtttcc tttgaaaacg
     5161 catactcgga tagaaaagtc tcctcgcaag atggcgaaaa tatagacatc gtgtccattg
     5221 gagatttgct tacgattaag agaatcgccg ctcaggaatc tgcgactagg ttacacaagg
     5281 agttgaaaga cattggcttc ttggagagca gatcagagga acagtgagta aatactcata
     5341 tctgactcca acgctgtccc gaactttccg catgtgcaat agactttgat tttcttgtct
     5401 tgtctctttt tgcccatgag acgatttgtc aattttctca tcctggttcc tgcttacctt
     5461 atcttcggta taggtcttgt tgtgtcaata gaggcccctc ggggtcaggc ttgacttgtt
     5521 gactttgaga acaatggctg cgcctcatct gcacacacca tccgccttat gcggctgtaa
     5581 cgtgagagtg gtgagtgtaa ttacagcctt gctggtcacg ctgggcctcg gaacactgac
     5641 cggctgtacc cgcccaagcc caagtcgctg cccgcggcgc gcaggtgatg ccgttcgatc
     5701 tggagcagac gattcacgtg tttcagcggc tggaggacgg tcccatcggt caccatcggg
     5761 gtcaggcttg acttgttgac tttgagaaca atggctgcgc ctcatctgca cacaccatcc
     5821 gcc
//
LOCUS       DBNGTT010000401         1751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2267202, whole genome shotgun sequence.
ACCESSION   DBNGTT010000401 DBNGTT010000000
VERSION     DBNGTT010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2267202"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1751
                     /locus_tag="AC16BC_09115"
     CDS             <1..>1751
                     /locus_tag="AC16BC_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3241819.1"
                     /translation="DGSTPTTNDPIRGRGSFQRIVDGIRAAIGGALSVTVSTVITETN
                     TDDLPEITRLVARLGGGTHHLLWLHKRGRANNGADRTPSVDRVIDVVRRCREVGRSVG
                     VLVDNHDAVKARLRYPAGTKRDLASAGVSSLCVYADGQVYPSAAMANVPELACGSILE
                     QSLRDIWMASDVARAFASATVEDKAICKTCPLKFLCGGGDIEHAYFYGGSIAAHDPYC
                     ELHQAMISDALQELTEQRRALVTNRKSGFSAPIIYTGMGESAVTCATDETPGEVLTSR
                     SECVLTFDLDAPRKVVREFYGNAAETPQEELCCPVQPNPDDPAHIPKAVVERFYGCGS
                     PVGAAGITPGEITLDLGSGAGIDVFIAAKKVGPTGRAIGVDMTPQMLAVARDAQRDVA
                     AALGYDVVEFRDGFLEQIPAESKSVDVVTSNCVINLSPDKPRVFADMWRVLKDHGRIV
                     VADIVADRDVPPRHRKDPRLWGECISGALTEEEFLAALERAGFYGLQVLKKSFWKEVE
                     GFQFFSVTVRGFKFEKTSGCVHAGQTATYVGPFKGVGDEEGHWFPRNVAIDVCTDTAA
                     KLSHAPYAGLFLVTRVE"
BASE COUNT          311 a          557 c          594 g          289 t
ORIGIN      
        1 tggacgggtc cacgcccacg accaacgacc ccatccgagg gagaggaagt tttcagcgga
       61 tcgtggacgg catccgcgcc gcgatcggag gtgcactctc ggtcacggtc agcaccgtga
      121 tcaccgaaac caacaccgac gaccttccgg agatcaccag gctcgtcgcc cgcctcggcg
      181 gcgggaccca ccacctgttg tggctgcaca agcgaggacg cgcgaacaac ggcgctgatc
      241 gcacgccgtc cgtcgaccgc gtgatcgacg tggtccgtcg ctgccgcgag gtgggacgat
      301 cagtgggtgt gctggttgat aaccacgacg ccgtgaaagc ccggctgcga tacccggcag
      361 gcaccaaacg cgatttggcg agcgccgggg tctccagttt gtgcgtgtat gcggacggcc
      421 aagtctaccc ctcggcggcc atggccaatg tcccggagct cgcgtgtggg tcgatcctcg
      481 aacagtcgct gcgcgacatc tggatggcga gcgacgtggc gcgtgcgttc gcctccgcaa
      541 ccgtcgagga caaggcgatt tgcaagacct gcccgctgaa atttctctgc gggggcggtg
      601 acatcgagca cgcctacttc tacggcgggt cgatcgccgc gcacgaccct tactgcgaac
      661 tacaccaagc catgatctcg gatgccctgc aggagctcac cgagcagcgg agagcgctcg
      721 tcacgaaccg caagtccggt ttctcggcgc cgatcatcta caccgggatg ggtgaatccg
      781 ccgtgacgtg cgcgaccgac gagacgcccg gcgaagtgct gaccagtcga tccgagtgcg
      841 tgttgacgtt cgacctggac gcgccccgca aggtggtccg cgagttctac ggcaatgccg
      901 cggagacgcc gcaggaagaa ctgtgctgcc cggtgcagcc caacccggac gacccggcgc
      961 acattcccaa ggcggtcgtc gaacgatttt acggctgtgg atcaccggtg ggcgcggcgg
     1021 gaatcacacc gggcgagatc acgctggatc tgggcagcgg cgcgggtatc gacgtgttca
     1081 tcgccgcgaa aaaggtcggg ccgaccgggc gggccatcgg cgtggacatg acgccccaaa
     1141 tgctcgcggt ggcgcgcgac gcgcagcgag acgtcgcggc cgcgctgggt tatgacgtgg
     1201 tggagttccg cgacggattc ttggagcaga ttccggccga gtccaaatcc gtggacgtgg
     1261 tgacgtccaa ctgcgtgatc aatctctctc ccgacaagcc ccgcgtgttc gcggacatgt
     1321 ggcgggtcct caaggaccac ggccgcatcg tggtcgcgga catcgtggcg gatcgcgacg
     1381 tccccccgcg ccatcgcaag gacccccgcc tgtggggcga atgcatcagc ggcgcgctga
     1441 ccgaggagga gttcctggcc gcgttggaac gcgccggatt ctacggcctg caggtcctca
     1501 aaaagtcgtt ttggaaagag gtcgagggct ttcagttttt ctcggtcacg gtccgcggtt
     1561 tcaagttcga gaagacttcg gggtgcgtcc acgcggggca gaccgcgacg tacgtggggc
     1621 catttaaggg cgtcggcgac gaggaaggcc actggttccc gcgcaacgtg gcgatcgacg
     1681 tctgcaccga tactgccgcg aagctctcgc acgcgccgta cgccgggctg ttcctcgtga
     1741 cgagggtcga g
//
LOCUS       DBNGTT010000402         1834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2650309, whole genome shotgun sequence.
ACCESSION   DBNGTT010000402 DBNGTT010000000
VERSION     DBNGTT010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2650309"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="AC16BC_09120"
     CDS             complement(<1..594)
                     /locus_tag="AC16BC_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241820.1"
                     /translation="MAYHTHRTTSAIFLGFLGGAIAALLAGWWVLSSHLATAGVMDRS
                     NRMANMTEMEGMDMSGGNTQAPVTLSATRRQAIGVKTGVVEKRPLETVIRAVGAVTYD
                     ERRVRQVSLRVAGWVTNLAVNYTGQAVRAGEPLLTIYSPELAATQAEYRLALRTQARI
                     GQDVMEPIRAGSDAQVQAAADRLRRLNMSREQIEALKQ"
     gene            complement(622..>1834)
                     /locus_tag="AC16BC_09125"
     CDS             complement(622..>1834)
                     /locus_tag="AC16BC_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3241821.1"
                     /translation="AVLAAIADYSIGWSADQTLEELLARAMDANPTVIAARQRWEVAK
                     AQAPQARALEDPVVSVTEWDVPSDLNLGETGQTWYGIEQAFPSPGKRALRARIATFDS
                     EAAEQDSLAVTRRVRAQVKTAYARLYRVQQQIAVHREHQALMNELIQSALHRYAVGQA
                     TQQESLKAQIELSTLHASLLALEQEQQSLRIELNTLTGHPDDPLFFGPAQIEYRPLTV
                     ALDALEAQALEHRPELRAAGRMVARQDEAVALAQRGWWPDFMAEVSYWDVHDGPNRWM
                     LTGKMTLPLGASGKYRAKIAEETAGRMRAAAEVDAARNETASLVRDTFLKLKTSQSLI
                     DLYRHGIIVQAEQGLESARIAYTTGRTDFLNVIDAERQLRDARLAADLALADWAEQRA
                     ELERVVGVDLP"
BASE COUNT          291 a          607 c          582 g          354 t
ORIGIN      
        1 ctgcttcaac gcctcgatct gctcacgact catattcaga cgccggagcc gatcggccgc
       61 ggcctggacc tgggcgtccg agccggcccg gatcggctcc atcacgtctt gaccgatccg
      121 cgcctgggtg cggagggcca accggtactc ggcctgggtc gcggccagtt ccgggctgta
      181 gatcgtgagc agcggctcgc ccgcgcggac cgcctggccc gtgtaattca cggccaggtt
      241 ggtgacccat ccggccacgc gaaggctcac ctgtctgacg cgccgttcgt catacgtcac
      301 ggcgcccacc gcgcggatca ccgtctccaa tggacgcttc tccacgaccc cggtcttcac
      361 gccgatcgcc tggcggcggg tcgccgagag cgtgacgggc gcctgcgtgt tcccacccga
      421 catgtccatg ccctccatct cagtcatgtt cgccatcctg tttgagcggt ccatgactcc
      481 ggccgtggcc agatggctcg acaggaccca ccaccccgcg agcaaggcgg cgatcgcgcc
      541 cccgaggaag cccaagaaga ttgctgacgt ggttcgatgc gtgtgatatg ccatggttgt
      601 ttgtcctttc cgtattgagg tttaggggag gtcgacgccg accacgcgtt ccagttcggc
      661 gcgttgctcg gcccagtcgg cgagcgcaag gtccgccgcg agccgcgcgt cccgcagttg
      721 acgttccgcg tcgatcacat tcagaaagtc ggtccggccg gtcgtgtacg cgatccgcgc
      781 cgactccaaa ccctgttcgg cctgaacgat gatgccgtgg cgatatagat cgatcagcga
      841 ctgagaagtc ttgagcttga gaaacgtgtc ccgaaccaag gacgcggttt cgttcctggc
      901 ggcgtcgact tcggccgcgg cccgcatccg gcccgcggtt tcctccgcga tttttgcccg
      961 atattttccg gaagcaccta agggaagcgt catctttccg gtgagcatcc agcgattcgg
     1021 cccgtcgtgc acgtcccagt acgaaacttc cgccatgaaa tctggccacc acccgcgttg
     1081 cgccagcgca acggcttcgt cctgacgcgc gaccatacgc ccggccgctc gaagctcggg
     1141 acggtgttcc agggcctgcg cctccaacgc atcgagggcg accgtgaggg ggcgatactc
     1201 gatctgcgcc ggaccgaaga acaacgggtc gtcgggatgg ccggtgagcg tgttcagttc
     1261 gatgcggaga ctctgttgct cttgttcgag cgccaggaga ctcgcgtgca atgtggaaag
     1321 ttcgatttga gctttgaggc tctcctgttg ggtcgcctgt cccaccgcgt agcgatgcaa
     1381 tgcgctctgg atcaactcgt tcatcagggc ctggtgctcg cggtgaacgg cgatctgttg
     1441 ctggacgcga tacagccgtg cataggccgt tttcacctgg gcgcggaccc gccgcgtcac
     1501 ggcgagagag tcctgttccg cggcctcgga atcgaacgtc gcgatacggg ctcgtaaggc
     1561 ccgcttgccc ggggagggga acgcctgttc gattccgtac caggtttggc cggtctcccc
     1621 gaggttcaaa tccgacggaa catcccactc ggtgacgctg acgaccgggt cctcgagcgc
     1681 gcgcgcctga ggcgcttggg ccttggccac ttcccaccgt tgccgggccg cgatgacggt
     1741 cgggttggcg tccatggccc gagcgagcag ttcttcgagc gtctgatcag cggaccaccc
     1801 gatggaataa tcagcgatcg cggcgagcac tgcc
//
LOCUS       DBNGTT010000403         3035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1756734, whole genome shotgun sequence.
ACCESSION   DBNGTT010000403 DBNGTT010000000
VERSION     DBNGTT010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1756734"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1369)
                     /locus_tag="AC16BC_09130"
     CDS             complement(140..1369)
                     /locus_tag="AC16BC_09130"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR3241822.1"
                     /translation="MKAELIAVGTELLWGDRVDTNTVTLADALAACGIEVAAKTVVGD
                     DEESLARALSDAMARAPLVILTGGLGITHDDVTIGAIARVAKRRLVLRDDVLAEIRNA
                     YRRRGREAPPASERQALLPVGAEALPNPVGIAPGLRLTEGGAHVVALPGVPAEMRAMF
                     DASVAPWLADLGAGAPIPRRTVHLFGLGELEVDRRLSDLFRDAACETGILASPRGVEI
                     RLRATGKDRATDELDRLVAEIDRRLEDAVYAHDGVPMEAVVGDLLRAKGWTVAVAESC
                     TGGLVGHRLTEVSGSSRYFVGGWVTYANEAKTRWLGVAPEQLAAHGAVSEPVAAMMAD
                     GARRAAGSDVALALTGIAGPEGGTPDKPVGTLVVGLADARGTETARWRFSGTRADTKL
                     AFSQFGLNALRRFLARP"
     gene            complement(1366..1827)
                     /locus_tag="AC16BC_09135"
     CDS             complement(1366..1827)
                     /locus_tag="AC16BC_09135"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR3241823.1"
                     /translation="MRERWAWWVATAGGVGTVPWAPGTAGSVVGAALGWWAGPALGLP
                     GYAAVTAVLFGLGVAAAGRTERAARRKDPSIVVIDEVVGMLVALCALPFRAGEVLAAF
                     LCFRLFDIAKPFPLRRLEAQPGGWGIMLDDLVAGVYANLAVRLGRWALGVS"
     gene            2177..2638
                     /gene="speD"
                     /locus_tag="AC16BC_09140"
     CDS             2177..2638
                     /gene="speD"
                     /locus_tag="AC16BC_09140"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HIR3241824.1"
                     /translation="MTKGGEKVHGLGTHLLAELKACNAKLLNDVKKVEMIMVEAAKTA
                     KATIVDVHFHKFSPFGISGVVVIAESHLAIHTWPEYGYAAVDVFTCGDVLQPEVAIEF
                     LIEKFQCKQPSIMEVRRGILSKGNVKLPHKTVPTSEATPVDDRAKELQMVS"
     gene            2664..>3035
                     /locus_tag="AC16BC_09145"
     CDS             2664..>3035
                     /locus_tag="AC16BC_09145"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermine/spermidine synthase domain-containing
                     protein"
                     /protein_id="HIR3241825.1"
                     /translation="MHGIRTMLYAAQTKYQQVEIMDTGSYGRCLVLDGKMQSSEADEF
                     IYHEALVHPALLTHPEPKRVFIVGGGEGATLREILRHRTVEHVLMVDIDQEVVERCKQ
                     FLPEWHQGAFDDPRVELKFLDA"
BASE COUNT          528 a         1123 c          957 g          427 t
ORIGIN      
        1 catcggatgc ccccggcccc gcgggccagg cgatcccgca gcgccgccag acccgcggcc
       61 cacgattccg ggacgtggac cgccacgaaa ctccgcaccg cgtcccgatc ttccccggtc
      121 cgcgcgcgtc cccccgctct cacgggcgcg cgagaaaccg ccgcagcgcg ttcagcccga
      181 attgggagaa cgcgagcttc gtgtccgccc gcgtgccgga gaaccgccat cgcgccgtct
      241 ccgtgccccg cgcgtcggcg agcccgacca ccaaggtgcc gaccggcttg tcgggagtgc
      301 cgccctcggg tcccgcgata cccgtcaacg ccagcgccac atcgctgccc gcggcccggc
      361 gcgcgccgtc ggccatcatc gcggccaccg gctcgctcac cgcgccgtgc gcggcgagtt
      421 gctccggggc cacgcccaac caccgcgtct tcgcctcgtt cgcgtaggtc acccagccgc
      481 ccacgaagta ccgcgaactg cccgacacct cggtgagccg gtgcccgacc aacccgccgg
      541 tgcacgactc cgccacggcc acggtccacc ctttcgcgcg cagcaggtcg ccgaccaccg
      601 cctccatcgg gaccccgtcg tgcgcgtaca ccgcgtcctc caaccggcgg tcgatctccg
      661 cgaccaggcg gtccagttcg tcggtcgcgc gatccttccc ggtcgcgcgg agccggatct
      721 ccacgcctct gggcgacgcc aggatcccgg tctcgcacgc ggcgtcgcga aagagatccg
      781 acaaccgccg atccacctcc agctcgccga gcccgaagag atgcacggtg cgccgcggga
      841 tcggcgcgcc cgcgccgagg tccgccagcc acggcgccac ggacgcgtcg aacatcgcgc
      901 gcatttcggc cggcaccccg ggcagcgcca ccacgtgggc cccgccctcg gtgaggcgaa
      961 gcccgggcgc gatgcccacc ggattcggca gcgcctcggc gcccaccggc aacaacgcct
     1021 gtcgctcgct cgccggaggc gcttcgcgtc cccgacgccg gtacgcgttg cgaatctccg
     1081 cgagcacgtc gtcacgcaac acgagacggc gtttggccac gcgcgcgatc gccccgatgg
     1141 tgacgtcgtc gtgcgtgatg ccgagtccgc cggtcaggat cacgagcggc gcgcgcgcca
     1201 tggcgtcgga caacgcgcgg gcgagcgatt cctcgtcgtc gcccaccacg gttttggccg
     1261 ccacctcgat cccgcacgcg gccaacgcgt ccgcgagcgt caccgtgttc gtgtcgaccc
     1321 gatcgcccca caacagttcg gtgccgaccg cgatgagttc tgccttcacg acacgcccag
     1381 cgcccaccgc cccagccgca ccgccaggtt cgcgtacact cccgcgacca gatcatcgag
     1441 catgatgccc catcccccgg gctgcgcttc gagccggcgg aggggaaacg gcttggcgat
     1501 gtcgaacagc cgaaaacaga gaaaagcggc cagcacctcg ccggcccgga aggggagcgc
     1561 acagagggcc acgagcatcc cgaccacctc gtcgatgacc acgatggagg ggtccttccg
     1621 gcgcgcggcc cgttcggttc gtccggcggc cgccacgccc agtccgaaga gcacggcggt
     1681 gaccgccgcg taccccggga gaccgagcgc gggccccgcc caccatccga gcgccgcgcc
     1741 gacgacactg cccgcggtgc cgggcgccca aggcacggtc ccgacccccc cggccgtggc
     1801 cacccaccat gcccatcgct cccgcaccgc gtgatctcgt cgcctcggcc gatcccgtcg
     1861 gaaaaggcgg cccattctag cacccggtcc ggggggaatc aaggcgagcc cacgaggatt
     1921 cggcgaccgg aatatcccac actggagtcg acgctttcgg cgccccgcgc gaccgcccgt
     1981 gcggcgccgg agcgcgtcgc aaattttttt cgcggcggca aaattttttc ttgacttgga
     2041 ccttgattca gttatagtac ggtccaaaat ttccgatgac gattcgcgtg gcgtgtcgcg
     2101 ccgcgcgcga gtcgaaaatg gggatcgatg cgggcgccgg acacggctct gcaagcggca
     2161 cccgcgggac ggcgcgatga cgaaaggggg agagaaggtg cacggattag gaacccatct
     2221 gttggcggag ctcaaggcgt gcaatgccaa gctgctcaac gatgtgaaga aggtcgagat
     2281 gatcatggtg gaggccgcga agaccgcgaa ggccaccatc gtggacgtgc actttcacaa
     2341 gttctcgccg ttcgggatca gcggggtggt ggtgatcgcg gagtcgcacc tggcgatcca
     2401 cacctggccc gaatacggct acgccgcggt cgacgtgttc acctgcggcg acgtgttgca
     2461 acccgaagtc gccatcgagt tcctgatcga gaagtttcaa tgcaagcaac cgtctatcat
     2521 ggaagtgcgg agaggcatcc tctcgaaggg caacgtgaaa ctgccccaca aaaccgttcc
     2581 gaccagcgag gcgacgcccg tcgatgaccg agccaaagag ctacaaatgg tttcttgact
     2641 tcctcaaccc tgaggaaggg catatgcatg gtatccgcac catgctgtat gccgcgcaaa
     2701 ccaagtacca acaagtcgaa atcatggaca ccggcagtta cggccggtgc ctggtgctgg
     2761 acggcaagat gcagtcgtcg gaagccgacg agttcatcta tcacgaggcg ctcgtccacc
     2821 ccgcgctgct gacgcacccc gaacccaaac gcgtgttcat cgtgggcggg ggcgagggcg
     2881 ccacgctccg ggaaattctc cgccaccgca cggtcgaaca cgtgttgatg gtggacatcg
     2941 accaggaagt agtcgagcgg tgcaaacaat ttctccccga gtggcaccag ggcgcgttcg
     3001 acgatccgcg ggtggagctc aagttcctgg acgcc
//
LOCUS       DBNGTT010000404         2104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_863643, whole genome shotgun sequence.
ACCESSION   DBNGTT010000404 DBNGTT010000000
VERSION     DBNGTT010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_863643"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /gene="ruvC"
                     /locus_tag="AC16BC_09150"
     CDS             complement(<1..388)
                     /gene="ruvC"
                     /locus_tag="AC16BC_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR3241826.1"
                     /translation="MRILGIDPGLNATGYGVVELEGSELCHVASGTIVVPATRALPAR
                     LNQLFESLREVCERYRPEEVAVENTFLAKNVAVALKLGQAKGVALLVAEQAKLPILEY
                     TPTEIKIAVTGHGGAAKEQVQYMVGRL"
     gene            complement(427..1179)
                     /locus_tag="AC16BC_09155"
     CDS             complement(427..1179)
                     /locus_tag="AC16BC_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3241827.1"
                     /translation="MSGHSKWATTKHKKAAADSKRGKIFTKLIKEITVAARIGGGDPD
                     GNPRLRLAIQKSKEANMPADNIKRAVMKGTGELPGVTYEEFVYEGYGPGGTAILVEIV
                     TDNKNRTVSEIRHVFSKNGGNMGEAGCVAWMFQKRGYITVAKNKADEDTLMTLALDAG
                     AEDMKSDDEEQFEIFTAPSDVEAVKKALEDKKVPVALAEVAQIPQTYIKLEGKDAEQM
                     IKLMDALEDHDDVQKVHANFDIDQAVLEKVLG"
     gene            complement(1459..>2104)
                     /locus_tag="AC16BC_09160"
     CDS             complement(1459..>2104)
                     /locus_tag="AC16BC_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HIR3241828.1"
                     /translation="AFRFALDPAVDFRFIAGQYVMVDVPKGETVVRKAYSIVSPPFQA
                     GSIDLCIKHVEGGYVSTYFHEQFRVGDALTISDARGNFVDRGGDRERVYVAAGTGIAP
                     IRSMILGLYDNGFDGPMTLYFGVRHEDEILYETDWRRLEAAHTGFRFIPAVSRPRAWT
                     GRVGWVQNALRETLTDPATKTAYVCGLVPMVKEVKALLIELGVPREQIHTEKYT"
BASE COUNT          353 a          681 c          627 g          443 t
ORIGIN      
        1 gcaagcggcc gaccatgtat tggacctgtt ccttggccgc gccgccgtgg ccggtgaccg
       61 cgattttaat ctcagtcggt gtgtactcca ggatgggcag cttggcctgc tccgccacca
      121 ggagcgccac gcccttggcc tgaccgagct tgagcgccac ggccacgttc ttggccaaga
      181 acgtgttttc caccgccacc tcttcggggc ggtagcgttc gcacacttcg cgcagggatt
      241 cgaagagttg attgaggcgc gcgggcaacg cgcgcgtggc gggaaccacg atggtgccgg
      301 acgccacgtg gcagagctcg gatccttcca gctccacgac gccgtagccg gtggcgttca
      361 gacctggatc aatccccaaa atccgcatgg ccggccctct ctcccttgag ccgcggacgg
      421 tgcgtcctac cccagcacct tctccaacac ggcctggtcg atgtcgaagt tcgcgtggac
      481 cttctgcacg tcgtcgtgat cttccagcgc gtccatcaac ttgatcatct gctcggcgtc
      541 cttgccttcg agcttgatgt aggtttgcgg aatctgggcc acctcggcga gcgcgaccgg
      601 caccttcttg tcttccaggg ccttcttgac cgcttccacg tcggacggcg cggtgaaaat
      661 ctcgaactgc tcctcgtcgt cggatttcat gtcttcggcg cccgcgtcca gggcgagggt
      721 catgagcgtg tcttcgtccg ccttgttctt ggccaccgtg atgtagccgc gtttctggaa
      781 catccaggcc acgcagccgg cttcgcccat gttgccgccg ttcttggaaa acacgtggcg
      841 gatctccgac acggtgcgat tcttgttgtc ggtcacgatt tcgaccaaga tggcggtgcc
      901 gccgggaccg tacccctcgt agacgaattc ttcgtacgtc acgccgggga gctcgcccgt
      961 gcccttcatg accgcgcgct tgatattgtc cgccggcatg ttggcttcct tggacttctg
     1021 gatggccagg cgcaggcgcg ggttgccgtc cgggtcgccg cccccgatcc gcgcggccac
     1081 cgtgatctcc ttgatcagct tggtgaaaat cttgccgcgt ttggaatccg ccgcggcttt
     1141 tttatgcttg gtggtggccc atttagagtg gccggacatg acggaactcc gcggaattga
     1201 ctcgaaaatt tgcctggaaa acagcgtggt tgtaacacat gtttgacgag cgagtcaaac
     1261 gcgcgaacgg ctgctgaaaa agtccaccgg cggcgttgct tcgcgatccg caaggatgtc
     1321 aatttgtagc cgctccttcg gagcggggcg gattctcggt cgtcggaact cctcacgtac
     1381 aacccagtac gctccggggt tcctcctccc tgcggccttg ccgctggagc tttttgagca
     1441 gccgaggctt tcaccctgtt aggtgtattt ttcggtgtgg atctgctcgc gcggcacgcc
     1501 caactcgatc agcagggctt tgacttcctt gaccatgggc accaggccgc agacgtacgc
     1561 ggttttggtc gcgggatcgg tcaatgtctc gcgcagcgcg ttctgcaccc agcccacgcg
     1621 gccggtccat gcgcgcggac ggctgaccgc ggggataaaa cgaaacccgg tgtgcgcggc
     1681 ttcgagtcgg cgccaatcgg tctcgtagag gatttcgtcc tcgtgccgca cgccgaaata
     1741 caacgtcatg ggcccgtcga atccgttgtc gtacaagccg aggatcatgc tgcggatcgg
     1801 cgcgatgccg gtgcccgcgg ccacgtacac ccgctcgcga tcaccgccgc ggtccacgaa
     1861 attcccacgc gcatcggaga tagtcagagc gtcgcccacc cgaaactgtt cgtgaaaata
     1921 cgtggacacg tacccgcctt ccacgtgctt gatgcacagg tcgatggacc cggcctgaaa
     1981 cggcggagac acgattgaat acgccttgcg caccacggtc tcgcccttgg gcacatcgac
     2041 catcacgtac tgcccggcga taaaccgaaa atccaccgca ggatcgagcg cgaaccgaaa
     2101 cgcc
//
LOCUS       DBNGTT010000405         2619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1757613, whole genome shotgun sequence.
ACCESSION   DBNGTT010000405 DBNGTT010000000
VERSION     DBNGTT010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1757613"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="hrcA"
                     /locus_tag="AC16BC_09165"
     CDS             <1..983
                     /gene="hrcA"
                     /locus_tag="AC16BC_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3241829.1"
                     /translation="TPVGSRTLTKSMELGLSPATIRNVMADLEEMGYLAQPHTSAGRI
                     PTEKAYRVYVNDLLNAGPSDATDSTPANLHLAPADDLKDILQETSKLISHLSNYTGVV
                     VTPKSVTHRLKQVEFFRLGGRQVLAVFLMGDGFVHHKRIELDTDPAPEDLRRIADELN
                     TRFSGQDLAVIRRQLIEEMHEHKRDYDRLMERVLAAAQGTLSQQAGEVYVEGAGNILG
                     LPEFSDVERMKALFKTFEETYMMSTLIEKSLNTDGVRVYIGSDNAALGANDLSLVVAN
                     YRCGDHAHGTLGVLGPMRMEYDRVIPLVNRFAALLGECLQDQAGADPAHG"
     gene            1024..1557
                     /gene="grpE"
                     /locus_tag="AC16BC_09170"
                     /pseudo
     CDS             1024..1557
                     /gene="grpE"
                     /locus_tag="AC16BC_09170"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
     gene            1717..>2619
                     /locus_tag="AC16BC_09175"
     CDS             1717..>2619
                     /locus_tag="AC16BC_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR3241830.1"
                     /translation="MGKVIGIDLGTTNSCVAIVSGGDPVVIPNAEGSRTTPSVVAITD
                     KGERLVGQIAKRQAITNPEHTIYSVKRLMGRKFGSAQVREAIKRLPYKVVEAKNGDAH
                     VEIRGKAYSPPEVSAMILQKMKQTAEDYLGEPVTEAVITVPAYFDDSQRQATKDAGEV
                     AGLKVLRIINEPTAASLAYGLEKKKDEKIAVYDLGGGTFDISILEIGDGVFEVKSTNG
                     DTYLGGDDFDLRIMDWLVDEFKRDQGIDLRKDKMALQRLKEAAEKAKIELSASQETEI
                     NLPFITADASGPKHLVVKLSRSKME"
BASE COUNT          610 a          789 c          813 g          407 t
ORIGIN      
        1 cgacgccagt cggatcccga acactcacga aaagcatgga actgggcctc agccctgcca
       61 ccatccgaaa cgtgatggct gaccttgagg aaatggggta cctcgcgcaa ccgcacactt
      121 cggccggacg cattcccacg gaaaaggcct atcgcgtcta tgtgaacgat ctgctgaatg
      181 cgggaccaag cgacgccacg gactcgacgc cggccaacct gcatctcgct ccggccgatg
      241 atctcaaaga catactacag gagacgtcca agctgatctc gcacttgtcg aactacaccg
      301 gcgtggtcgt cacgccaaaa tccgtgacgc accggctcaa gcaagtcgaa ttcttccgct
      361 tgggggggcg acaagtcctg gcggtgttct tgatgggcga tggattcgtc caccacaagc
      421 ggatcgaact ggacacggac cccgcccccg aggacctgcg ccggatcgcg gacgagttga
      481 acacccgctt ttccggacaa gacctcgccg taatccgcag acagctcatc gaggagatgc
      541 acgagcacaa acgtgattac gatcggctca tggagcgagt gctcgcggcg gcgcaaggaa
      601 ccctctcgca gcaagccgga gaagtgtacg tggaaggcgc gggcaacatt ctcggcctcc
      661 cggaattctc ggacgtggaa cgcatgaaag cgctcttcaa aacgttcgag gaaacctaca
      721 tgatgagcac cctgatcgaa aagagcttga acaccgacgg cgtgcgggtc tatatcgggt
      781 ccgacaacgc ggccctgggg gccaacgacc tcagcctggt ggtcgcgaac tatcggtgcg
      841 gcgaccacgc gcacggcacg ctgggggtgt tgggcccgat gcgcatggag tacgaccggg
      901 tgatcccgct ggtcaaccga ttcgcggcat tgctgggcga gtgtttgcaa gaccaggccg
      961 gcgccgaccc ggcgcacgga taacccgccc gccgcaccac gcgatacgga tcgagtcagg
     1021 agcacgatgg agccagaaca ggaggaacac gtgacgcatg ccggggccac gcccgagaac
     1081 ggcgcggact gggagacgca actcgcggca aaagaacgcg aatgctcgga cgccaaggat
     1141 cggtatctgc ggctgtacgc cgagtttgag aattacaaac ggcttgcgca acgcgatcaa
     1201 caagactaca ccaagttcgc ggccgaaaaa gtgctcaaag aactgctccc gatcgtggac
     1261 aatttggagc gggccgtctc gcacgcacgc ggcgcgcaag cggacgcgtc gatcgtggac
     1321 ggcatggcgc tgatcctccg gcaatttcac gacgcgctga gcaagaacgg gtgcgaaccg
     1381 atcgaggcgg tcggcaaacc gttcgacccc gcgtttcacc aggcgctcgc gcaggttgaa
     1441 tcgaccgagc acaaagcgga caccatcgtc gaggaggcgc aacgcggata tctgctgcac
     1501 ggtcggattc tccgcccgtc gttggtcacg gtcagcaaga aaccgggctc gacctgacat
     1561 cgcggcgact tgcgggaagc cacggggaca tgataaaacg acgcggttta cgcgccatct
     1621 tcaaagacga tccacgcggc gtccgaaccg tcgtccccac gcggaacgcc gtattcacgg
     1681 tttgtctcga tatcacggag tgtgaaagga gagtgtatgg gtaaagtcat cggcattgat
     1741 ctcggcacca ccaactcctg cgtggcgatc gtctccgggg gcgacccggt ggtgatcccc
     1801 aacgctgaag gcagccgcac cacgccgtcg gtcgtcgcca tcacggacaa gggcgaacgg
     1861 ctggtcgggc aaatcgccaa acggcaggcg atcaccaacc cggaacacac catttactcg
     1921 gtgaaacggc tgatgggccg caagttcggg tcggcgcagg ttcgcgaggc catcaaacgc
     1981 ctgccgtaca aggtggttga ggccaagaac ggcgatgccc acgtggagat ccgcgggaaa
     2041 gcgtacagcc cgcccgaagt ctcggcgatg attcttcaga aaatgaagca gaccgcggag
     2101 gattatctgg gcgagccggt cacggaagcc gtgatcacgg tccccgcata tttcgacgac
     2161 agccagcgcc aggccaccaa ggacgcggga gaggtcgcgg gcctgaaggt cttgcgcatc
     2221 atcaacgaac ccaccgccgc gtcgcttgca tatggtttgg aaaagaaaaa agacgagaag
     2281 atcgcggtgt acgaccttgg cggcggcacg ttcgatatct cgattctcga gatcggcgac
     2341 ggcgtgttcg aggtcaaatc caccaacggc gacacgtacc ttggcggcga cgacttcgat
     2401 ctccggatca tggattggct ggtcgacgag ttcaaacgcg atcagggcat tgacctgcgc
     2461 aaagacaaaa tggcgctgca gcggctgaag gaagctgcgg agaaggccaa gatcgaattg
     2521 tcggcgtccc aggaaaccga aatcaacctc cccttcatca cggccgacgc gagcggaccc
     2581 aaacacctgg tcgtgaaact gagtcggtcg aagatggag
//
LOCUS       DBNGTT010000406         2910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2523464, whole genome shotgun sequence.
ACCESSION   DBNGTT010000406 DBNGTT010000000
VERSION     DBNGTT010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2523464"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2480
                     /gene="secA"
                     /locus_tag="AC16BC_09180"
     CDS             <1..2480
                     /gene="secA"
                     /locus_tag="AC16BC_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3241831.1"
                     /translation="SLDALLPEAFAVVREASRRVLGMRHFDVQLIGGIVLHEGKIAEM
                     KTGEGKTLVATLPVYLNALAGQGVHVITVNDYLARRDSQWMGEIYRFLGLTVGVIQHD
                     LDDAARQAAYAVDVTYGTNNEFGFDYLRDNMKFDPRHFVQRPLSFAIVDEVDSILIDE
                     ARRPLIISGPAEDSTELYYKINRVIPHLKAEADFTIEEKTKTVALTEEGNAKVERFLG
                     VENLYDLGNMSLVHHVHQALRAHSLYKRDVDYVVKDGEVVIVDEFTGRLMPGRRWSDG
                     LHQAVEAKEGVKIANENQTLATITFQNYFRMYKKLSGMTGTADTEAGEFAKIYNLDVM
                     VIPPNRRMVRRDHADVVYRTEAEKFQAITEEVVECHQRGQPVLVGTISIEKSERLSAL
                     LKRQGVRHAVLNAKFHEKEAEIIAQAGRKGTVTIATNMAGRGTDILLGGNSDFLFKQA
                     VARDSDLTAAEHEADRLRLNEQFRAECEADKEAVVALGGLHIIGTERHESRRIDNQLR
                     GRAGRQGDPGSSRFYLSLDDDLLRIFGSDRISGLMQRLGMEEGVPIEHRMVTRAIETA
                     QKKVEAHHFDIRKQLLEYDDVMNKQREVIYDQRRQVLSGEPFVDGIHEMVEDLVAGMV
                     AQYCSDTTYAEEWDVKGLAESVGAQFSITVDPSAWADSGAEAIKDDILSRVKDAYRNK
                     EKEFGPGVLRELEKAVMLRVIDTHWKDHLLTMDYLKEGIGLRGYGQKDPLVEYKREGY
                     DLFVGLIDRIKVDAVGHLFRIQAVRQDQVAPRPQAPRPQGMILGAPAAPATAGGSVPV
                     VRERERVGRNDPCPCGSGKKYKKCHGV"
     gene            2543..>2910
                     /locus_tag="AC16BC_09185"
     CDS             2543..>2910
                     /locus_tag="AC16BC_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3241832.1"
                     /translation="MRSQLHAKAKQLTFFLNTAKALTSSLELATVLDVVMDRARRLVP
                     CERSTLWLVDEVDGRRELRPHGAHHGGHGRNRLHPIPWGSGPSGWVAQHARPLLIQDR
                     SLPLPDGLRGWTPPRTRVQS"
BASE COUNT          607 a          794 c          968 g          541 t
ORIGIN      
        1 agtcgttgga tgccttgttg ccggaagctt tcgccgttgt ccgggaggcg tcgcgtcggg
       61 tcctcgggat gcgtcatttc gacgtgcagt tgatcggcgg gattgtactc cacgaaggca
      121 agatcgccga gatgaagacc ggcgaaggga aaacgctggt ggcgacgctc ccggtctacc
      181 tgaatgcgct ggccggccag ggcgtgcatg tgatcaccgt caatgattac cttgcccgtc
      241 gtgacagcca atggatgggg gagatctatc ggttcctggg gttgaccgtg ggggtcatcc
      301 agcatgatct ggacgacgcc gcgcggcaag ctgcgtacgc ggtggatgtg acctatggga
      361 ccaacaatga atttgggttc gactatctgc gcgacaacat gaagttcgac ccccgccatt
      421 tcgtgcaacg tccgctgagc ttcgcgatcg tggatgaagt ggacagcatt ttgatcgacg
      481 aagcccggag gccgttgatt atttccgggc cggccgagga ttcgaccgag ctctattaca
      541 aaatcaatcg cgtcatcccg cacctgaagg ccgaagccga tttcaccatc gaagagaaga
      601 ccaagaccgt cgcgttgacc gaggaaggga acgcaaaagt tgaacgcttc ctgggggtgg
      661 aaaatctcta cgacctgggc aacatgtcgc tggtccatca cgtccatcag gcgctcagag
      721 cacactcgtt gtataagcgg gacgtggatt acgtggtcaa ggacggcgag gtggtgattg
      781 tcgacgaatt taccggtcgg ctgatgcctg gccggcgatg gagcgacggt ctccatcagg
      841 cggtagaggc caaagaaggg gtcaagatcg ccaacgagaa ccagacgctc gcgacgatca
      901 cgtttcagaa ttacttccgc atgtacaaga agctcagcgg gatgacgggc actgcggata
      961 ccgaagcggg tgagttcgcg aagatttata acctggacgt catggtgatt cctcccaacc
     1021 gccggatggt gcggcgcgac cacgccgatg tggtgtaccg gaccgaagcc gagaagttcc
     1081 aagcgattac ggaggaagtc gtggagtgcc atcaacgagg ccaaccggtg ttggtgggca
     1141 caatatcgat cgaaaagtcc gagcggctct ccgccttgct gaaacgacag ggcgttcgcc
     1201 acgcggtgct caacgcgaag ttccatgaga aggaagccga aatcatcgcg caggccggac
     1261 gcaaaggcac ggtgaccatc gccaccaaca tggcgggacg cgggacggat attctgctcg
     1321 gtggcaactc cgactttttg ttcaagcagg ccgtggcccg tgattctgat ttgacggcgg
     1381 ccgagcacga ggcggatcgg ctgcggctca atgagcagtt ccgtgccgaa tgcgaggccg
     1441 acaaggaagc ggtcgtggcc ctgggcgggc tgcatatcat cggcaccgag cgtcatgaga
     1501 gccgacgtat cgacaaccaa ttgagggggc gcgcgggccg tcagggcgac cccgggtcgt
     1561 cgcggtttta cctgtcgctc gatgatgacc tgttgcggat tttcgggtcc gatcggatct
     1621 ctgggttgat gcagcgattg gggatggagg agggcgttcc gattgaacac cgcatggtca
     1681 cgcgggcgat cgaaaccgcc cagaagaaag tcgaagcgca ccatttcgat atccgcaaac
     1741 agttgctcga gtacgatgat gtgatgaaca agcaacgcga ggtcatttac gaccagcgtc
     1801 ggcaggtcct cagcggggaa ccgttcgtgg acggcatcca tgagatggtc gaggaccttg
     1861 tcgcgggcat ggttgcccaa tattgtagtg acaccaccta tgcggaagag tgggacgtca
     1921 aagggcttgc cgaatcggtc ggcgcccagt tttcgatcac cgtcgaccca tccgcgtggg
     1981 cggattccgg agcggaggcg atcaaggatg acattctgtc gcgagtgaag gacgcgtaca
     2041 ggaacaagga aaaagagttc gggccgggtg tactccgcga actcgagaaa gccgtgatgc
     2101 tccgcgtgat cgatacgcac tggaaggacc acctgttgac catggattac ctcaaggagg
     2161 gaatcgggct gcggggctac ggtcagaagg atccccttgt cgagtacaaa cgcgagggtt
     2221 acgacctgtt cgtggggttg attgaccgga tcaaggtcga tgccgtggga cacttgttca
     2281 gaatccaggc ggtgcgtcag gatcaggtcg cgccgcggcc gcaagcaccg cggcctcagg
     2341 ggatgatcct gggcgcgccg gcggcccccg cgacagccgg ggggagcgtt ccggttgtcc
     2401 gtgagcgaga gcgggtagga cgaaacgatc cgtgcccgtg cgggagcggg aaaaaataca
     2461 aaaagtgcca cggggtctga aacacctctc gcccggccgg ggtggcccct ccctcgccgg
     2521 cctgcagcgt gaaaacgctg cgttgcgttc ccaactccac gcgaaagcca aacagctcac
     2581 cttctttctc aatacggcca aggcgctcac gtcgtccctg gaactggcca cggtgttgga
     2641 cgtcgtgatg gaccgagcga gacgactggt cccgtgtgaa cggtcgacct tgtggctggt
     2701 tgatgaagtc gacgggcgtc gggagttgcg cccccatggg gcccatcacg gaggacacgg
     2761 tcggaatcgc ctgcatccga ttccgtgggg atcagggccg tccgggtggg tcgcccagca
     2821 cgccaggccg ttattgattc aggaccgttc gttgccgctc ccggatggac tgcgcggctg
     2881 gactcccccg cggacccggg ttcagtcgtt
//
LOCUS       DBNGTT010000407         2246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_224970, whole genome shotgun sequence.
ACCESSION   DBNGTT010000407 DBNGTT010000000
VERSION     DBNGTT010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_224970"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="AC16BC_09190"
     CDS             <1..1741
                     /locus_tag="AC16BC_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3241833.1"
                     /translation="FTLEAQEGRLFKPLAFTKTFSMLFASIVSITVVPLLMVALIRGH
                     ITPVAKNPINRALVALYAPVARLALRHGAAVIVIALALIAATVPVCRSLGSEFMPPLN
                     EGTILYMPTALPGISITKATQVLQQQNALLKTIPEVETVFGKIGRAETATDPAPLSMA
                     ETTITLKPESHWRPGMTWDGLIAEMDRLVHFPGMPNIWWMPIQTRTEMLATGVRSAVG
                     IKILGRDLAEIDRVGQEIEGALADLPGTRSAFAERVTGGYYLDIAVDRQRAARHNLTV
                     GDVERVIEVAIGGVNVTTTVEGRERYPVNVRYARALREDPESLKRVLVPTPGGATVPL
                     AQLANFTVTMGPSMVRDENGSLAGIVYVDVAGRDLAGYVEEAKQRVAERVTLPVGTSL
                     IWAGSYEYLLRAQERMKIVVPITLLLVFVLLYLNFRSVAQTLIVLLSIPFAVVGSVWL
                     LRALHYNVSVAVWVGIIALAGVAAETGVIMLIYLDEAFRRWEREGRVRTRAELRGAVI
                     EGAVLRVRPVVMTVSAVIFGLIPIMWSHGAGADVMKRIAAPMIGGMVTSTLLTLIVIP
                     VIYAWWRGRSLTA"
     gene            complement(1753..>2246)
                     /locus_tag="AC16BC_09195"
     CDS             complement(1753..>2246)
                     /locus_tag="AC16BC_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3241834.1"
                     /translation="SPFGVRSLSACHRDRPYSLECDGIEHRVAYDPAESRSGMFGGNS
                     NWRGPIWFPTNFLLIESLQKFHYFLGDDYTVECPSGSGKLMTLREVADELSRRLTRIF
                     LQGPDGRRPVYGDNAAFQRDPHWRDLVLFYEYFHGDRGMGLGASHQTGWTGLVAKLLQ
                     QLG"
BASE COUNT          369 a          701 c          778 g          398 t
ORIGIN      
        1 cttcacgttg gaggcgcagg aggggcgatt gttcaagccg ctcgccttca ccaagacgtt
       61 ctcgatgttg tttgcctcga tcgtgtcgat cacggtggtc ccgttgctta tggtggccct
      121 gatccggggc cacatcaccc cggtcgccaa gaacccgatc aatcgtgctc tggtcgcgct
      181 ttacgccccg gtggctcggc tggcgctgcg acacggggcg gcggtgatcg tgatcgcgct
      241 ggcgctcatc gcggcgaccg tgccggtttg ccgctcgttg gggtcggagt tcatgccgcc
      301 gctcaacgag ggcacgatcc tctacatgcc caccgcgctc ccggggattt cgatcaccaa
      361 ggccacgcag gtccttcaac aacagaacgc gttgctcaaa accatccccg aagtggagac
      421 cgtcttcggc aaaatcggcc gcgccgagac cgccactgat ccggcgccgc tgtccatggc
      481 ggagaccacc atcacgctca aacccgagtc gcactggcga cccgggatga cgtgggacgg
      541 cctcatcgcg gagatggacc gtctggtcca cttccccggg atgccgaaca tctggtggat
      601 gccgatccag acccggaccg agatgttggc gactggcgtg cgcagcgcgg tgggcatcaa
      661 gatcttgggg cgtgacctgg cagagatcga ccgcgtgggc caggaaatag aaggcgcgct
      721 cgcggacctg ccgggcacgc gcagcgcgtt cgccgagcgg gtgaccggcg gctactatct
      781 cgacatcgcg gttgaccgac agcgggccgc gcggcacaac ctgacggtcg gggacgtcga
      841 acgcgtgatc gaggtcgcga tcggcggcgt gaacgtcacc accacggtgg aaggccgcga
      901 acggtacccg gtcaacgtcc gatacgcgcg cgcgctgcgc gaggatcccg agagccttaa
      961 gcgtgtgctc gtgcccacgc ccggcggagc cacggttccg ctcgcccagc tcgcgaattt
     1021 taccgttacg atggggccgt cgatggtccg ggacgaaaac ggttcgctcg cgggcatcgt
     1081 ctacgtggac gtcgcggggc gcgatctggc cggctacgtg gaggaggcca aacaacgcgt
     1141 cgccgaacgg gtcacgctcc cagtcgggac gtctttgatc tgggcgggat cgtacgagta
     1201 cctgctgcgc gcgcaggagc ggatgaagat cgtggtgccg atcacgctgc tcctcgtctt
     1261 tgtgctgctc tatctgaact tccgctcggt cgcgcagacc ctgatcgtgc tgctctcgat
     1321 cccgttcgcg gtggtcggga gcgtgtggtt gctgcgggcg ctccactaca atgtgagcgt
     1381 cgcggtttgg gtggggatca ttgcgctggc gggcgtggcc gcggagacgg gggtgatcat
     1441 gctcatctat ctcgacgaag cgttccgccg ctgggaacgc gagggacgtg tgcgcacccg
     1501 cgccgaactg cgaggcgccg tgatcgaagg cgcggtgctg cgcgtgcgac cggtggtgat
     1561 gacggtttcc gccgtcatct tcggattgat tccgatcatg tggagccacg gcgcgggcgc
     1621 cgacgtgatg aaacggatcg cggccccgat gatcggcggc atggtgacgt ctacgctgtt
     1681 gaccttgatc gtgattcccg tgatctacgc gtggtggcgg gggcggtcac tgactgcttg
     1741 acggggacga ggtcagccca gttgctggag cagcttggcg accagcccgg tccagcccgt
     1801 ctgatggctc gcgcccaggc ccatgccgcg gtcgccgtgg aagtattcgt aaaacagcac
     1861 gaggtcgcgc cagtggggat cgcgttggaa cgcggcattg tccccgtaga cggggcgtcg
     1921 gccgtcgggg ccttgcagga agatgcgcgt caggcgccgg gagagttcgt ccgcaacttc
     1981 ccggagcgtc atcagtttgc ccgatcccga cgggcactcg accgtgtagt cgtcgcccag
     2041 gaagtagtgg aacttctgca gggactcgat gagcaggaaa ttggtcggga accagatcgg
     2101 gccccgccag ttggagttgc cgccgaacat cccggaacgc gactccgccg gatcgtacgc
     2161 cacccggtgc tcgatcccgt cacactccaa gctgtacgga cggtcgcgat ggcacgcgga
     2221 cagcgaacgc acaccgaagg gcgaga
//
LOCUS       DBNGTT010000408         6303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2780702, whole genome shotgun sequence.
ACCESSION   DBNGTT010000408 DBNGTT010000000
VERSION     DBNGTT010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2780702"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="AC16BC_09200"
     CDS             <1..366
                     /locus_tag="AC16BC_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3241835.1"
                     /translation="DELAAESMGVPTTRLKVMAFALGAAWAGVAGVCFAARYAFVSPE
                     SFTFFESVVILAMVVLGGMSSVAGVAIAAVAIVVLPELLRGVDDFRMVAFGAVLVALM
                     AWRPEGLARRAVARRARTA"
     gene            363..1160
                     /locus_tag="AC16BC_09205"
     CDS             363..1160
                     /locus_tag="AC16BC_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3241836.1"
                     /translation="MTGDQPLLHAQGVTKAFGGLTALDHVSMAIERGMIAALIGPNGA
                     GKTTFFNCLTGLAQPDAGAILFDGAELAGLTPHAVASRGITRTFQNVRLFGALSAREN
                     VLVGAYCRQRAGLFPSILRTARARAEAAEADRAAEHWLDFVGIGDVADVLAGELAYGV
                     QRRLEIARALASRPALLLLDEPAAGMNPTETQALTALIRRIRDTGVTVLLIEHDMKVV
                     MTVSDRVVVFDHGVKIAEGSPSAVQRDPAVIEAYLGTAAAAGSDAAR"
     gene            1147..1857
                     /locus_tag="AC16BC_09210"
     CDS             1147..1857
                     /locus_tag="AC16BC_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3241837.1"
                     /translation="MLRVEALRVAYGHIEAVKGIDLEIRAGEIVALIGANGAGKSTTL
                     MTISGAITAAGGAVRFQGEPVHGRTASEIVRRGIAHVPEGRRIFRRFTVMENLRVGAM
                     SRRHGEIAESLDRVFGYFPLLRDRRGQLGGTLSGGEQQMLAIGRALMARPTLLLLDEP
                     SLGLAPLMVERIFEIIRAIHGAGTTMLLVEQNAHTALALADRAYVMETGRIVLHGPSA
                     ALRRDPKVRAAYLGEETQ"
     gene            1854..2963
                     /gene="gcvT"
                     /locus_tag="AC16BC_09215"
     CDS             1854..2963
                     /gene="gcvT"
                     /locus_tag="AC16BC_09215"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR3241838.1"
                     /translation="MTTPTQPLLTTALHAAHRALGARLVDFAGWEMPISYAGTVAEHT
                     AVRRAAGVFDIGHMGRLRVRGSGATAYLESLVTRGVGDIPPGAARYALVCNEQGTILD
                     DIFVYRYAGDDWLLIVNGANRLKIAAWMQQHRPMGVTVEDRTLETGLLAIQGPRTREV
                     LDRLSIALPSPFRLHTFIETVRNGRPVLVARTGYTGEWGVEVMAEHAVVAAVWDEALE
                     RGKDLGLVPVGLGARDTLRLEMGYALYGHEIDETTTPLDAGLEWAVDFAKTDFIGKAA
                     LSAQRARGLTRRLVGFELIDKGVPRQGHPILADGAPVGVVTSGNLSPSLDKGVGMGYM
                     DAARAVDGAPIEIDIRGKRKRAVIMKPPFYKKGKS"
     gene            3213..4550
                     /gene="gcvPA"
                     /locus_tag="AC16BC_09220"
     CDS             3213..4550
                     /gene="gcvPA"
                     /locus_tag="AC16BC_09220"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR3241839.1"
                     /translation="MEYMPNTADDHRAMLATIGVARFEDLLTEIPARARLNRPLTLPP
                     ALSELEVRREIGGLLDRNAHADRSPCFLGAGAYDHAIPSIVPFLTSRSEFSTSYTPYQ
                     AEMSQGMLQTIFEFQTMVCELTGMDVANASMYDGASALAEAALMACRVTKRSRIVAAG
                     TIHPHHRAVLATYLSGISHPVHTVRIVDGRADLDDLRRSLTDDTAAVLVQHPNFMGNL
                     EDVAAIAEAAHGRGALAVASVDPLSLGLLAPPGFWGADVAVGEGQALGNPMSFGGPFV
                     GFMATKKDFMRQIPGRIVGATVDTKGRPGYCLTLQAREQHIRRERATSNICTNQALMA
                     LTASIYLATLGPSGLREVGEQCVAKSHYAAERIAAVKGFARAAAAPFFKEFVVRTPRP
                     PAEINAALLKAGIIGGLDLGRFDTAWTNHWLVCVTEKRTREEIDRLVEVIGNI"
     gene            4621..6093
                     /gene="gcvPB"
                     /locus_tag="AC16BC_09225"
     CDS             4621..6093
                     /gene="gcvPB"
                     /locus_tag="AC16BC_09225"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR3241840.1"
                     /translation="MPEPLLFEKSTTGRRGYALPALDVPTPAADTIPTGARRATPPAL
                     PQLSEVDVIRHYTRLSRENYGVDAGFYPLGSCTMKYNPKINEEMARQPGAALAHPLQG
                     EALSQGALRLMWELEQELKEVAGVDRVSLQPAAGAQGEFAGILMIRAYHAAKGSPRRR
                     VLIPDSAHGTNPASAALAGYEVQTVHSNAEGLVDVGDLAKLINEECAALMLTNPNTLG
                     LFEKEILEIARIVHDAGALLYMDGANLNALLGLVRPGDMGFDVVHFNLHKTFSTPHGG
                     GGPGAGPIGVKSPLIPYLPTPTVERDGDRFYFDENRPQSIGRLHGFYGNFGILVRAYT
                     YLRRLGAAGLADISRHAIINANYVRARLGGAYQVAYDRACMHECVLSAKRQRSKGVHA
                     WDIAKRLIDYGIYPPTINFPMVVEEAIMVEPPESESRQTIDAFCDAMLAIAKECEEQP
                     DLVKGAPHTTPRSRLDEVLAARKPVVRWSPHPSLPLKGGG"
     gene            6111..>6303
                     /locus_tag="AC16BC_09230"
     CDS             6111..>6303
                     /locus_tag="AC16BC_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3241841.1"
                     /translation="MLSHDILIIGGGLAGMRAAIEVPSDLDVALISKVHPLRSHSVAA
                     QGGINAAIDPQDSWESHTFD"
BASE COUNT         1030 a         2081 c         2166 g         1026 t
ORIGIN      
        1 gacgaactcg ccgcggaatc catgggcgtg cccaccaccc gcctcaaggt catggctttt
       61 gccttgggcg cggcgtgggc cggcgtggcc ggcgtgtgtt tcgccgcccg gtacgcgttc
      121 gtctcacccg agagcttcac cttcttcgag tcggtcgtga tcctcgcgat ggtggtgttg
      181 ggcggaatgt ccagcgtcgc gggcgtggcg atcgccgcgg tggcgatcgt ggtgctgccc
      241 gaactgctcc ggggcgtgga cgactttcgc atggtggcgt tcggcgcggt cctggtggcc
      301 ctgatggcct ggcggcccga ggggctggcc cgccgcgccg tcgcccgccg ggcgaggacc
      361 gcgtgaccgg cgatcagccc ctcctgcacg cgcagggcgt gaccaaggcg ttcggcggcc
      421 tcaccgcgct cgaccacgtg agcatggcga tcgaacgcgg gatgatcgcc gccctcatcg
      481 gccccaacgg cgcgggcaag accacgttct tcaactgtct gaccggcctg gcgcaaccgg
      541 acgcgggggc gattctgttc gacggcgcgg agctggcagg gctcactccg cacgcggtcg
      601 cgtcgcgcgg gatcacgcgc acgtttcaaa acgtccggtt gttcggcgcg ctgagcgcgc
      661 gcgagaacgt gttggtgggc gcgtactgcc gccagcgggc cggattgttc ccgtcgatcc
      721 tgcggaccgc gcgcgcgcgc gccgaagccg ccgaggcgga ccgggccgcg gagcattggc
      781 tggacttcgt ggggatcggc gacgtggccg acgtcctcgc gggggaattg gcctacggcg
      841 tgcagcgccg gttggaaatc gcccgggcgc tggccagccg acccgcgctg ctgttgctcg
      901 atgagccggc cgccggcatg aatcccaccg aaacccaggc gttgaccgcg ctgatccgcc
      961 ggattcgcga cacgggcgtg accgtgctgc tcatcgaaca cgacatgaag gtggtcatga
     1021 cggtctcgga ccgcgtggtg gtgttcgacc acggggtgaa gatcgccgag ggttctccga
     1081 gcgcggtgca gcgcgatccg gccgtgatcg aagcgtacct ggggaccgcc gcggccgcgg
     1141 gttccgatgc tgcgcgttga ggcattgcgc gtggcatacg gccacatcga agccgtcaaa
     1201 ggcatcgacc ttgagatccg cgcgggcgaa atcgtggcgc tcatcggcgc gaacggcgcg
     1261 ggcaagagca ccacgctgat gacgatctcc ggcgcgatca cggcggccgg cggagccgtc
     1321 cggttccagg gcgagccggt ccacggccgc acggcctccg agatcgtacg ccgcggcatc
     1381 gcgcacgtgc ccgaggggcg gcggatcttc cgccgcttca cggtcatgga aaatctccgg
     1441 gtcggcgcga tgtcgcgccg ccacggcgag atcgcggagt cgctcgaccg ggtcttcggc
     1501 tatttcccgt tgttgcgcga tcgacgcggg cagttgggcg gcacgctcag cggcggagaa
     1561 caacagatgc tggcgatcgg ccgcgcgctg atggcccggc cgaccctgct gctgctcgat
     1621 gaaccgtcgc tcggactggc cccattgatg gttgagcgaa tttttgagat cattcgggcc
     1681 attcacggag cggggactac gatgctgctg gtcgaacaga acgcgcatac cgcgctggcg
     1741 ctcgcggatc gtgcctatgt catggaaacc gggcggatcg tgttgcacgg tccgtccgcc
     1801 gcgctgcgac gggacccgaa ggtccgcgcg gcgtatctcg gagaggagac gcagtgacga
     1861 cgcccacgca acccttattg accaccgccc tgcacgcggc gcaccgggcg ctgggcgcca
     1921 ggctggtgga tttcgccggc tgggagatgc cgatctcgta cgccggaacc gtggccgaac
     1981 acacggcggt ccgccgcgcc gcgggcgtgt tcgatatcgg tcacatggga cgcctgcgcg
     2041 tccgcgggtc gggcgccacg gcgtacctgg agtcgctggt gacgcgcggc gtgggagaca
     2101 ttccgcccgg cgcggcccgc tacgccctgg tgtgcaacga gcaaggcacg atcctggacg
     2161 acattttcgt ctaccggtac gccggagacg actggctgct gatcgtcaac ggcgccaacc
     2221 gcctcaagat cgcggcttgg atgcaacagc accgcccgat gggcgtcacg gtcgaggacc
     2281 gcacgctgga aaccgggctc ctcgcgatcc agggaccgcg cacgcgcgag gtcctcgatc
     2341 ggctgtcgat cgcgctgccc tcgccgttcc gactgcacac gttcattgaa accgttcgga
     2401 acggacggcc ggtgctggtc gcgcgcacgg gctacaccgg ggaatggggc gtggaggtca
     2461 tggccgaaca cgcggtagtc gccgcggtgt gggacgaagc gctcgagcgg ggcaaggatc
     2521 tgggtctggt tccggtgggg ctcggcgcgc gggacacgct ccgattggag atggggtacg
     2581 cgttgtacgg ccacgaaatc gacgagacca ccacgccgct ggacgcgggg ctggagtggg
     2641 ccgtggattt cgccaagacg gattttatcg ggaaggccgc gttgtcggcc cagcgcgctc
     2701 gcgggctcac ccggcggctg gtgggattcg aactgatcga caagggcgtg ccgcgtcaag
     2761 gccacccgat cctggccgac ggcgcgccgg tcggcgtggt gaccagcggc aatttgtcgc
     2821 ccagtctcga caagggcgtt ggaatggggt atatggatgc ggctcgcgcc gtggacggag
     2881 cgccgatcga aatcgatatc cgcggcaagc gcaaacgggc ggtgatcatg aagccgccct
     2941 tctataagaa gggcaagagt taggatgttc aagggttcaa tgttcacgaa gccctaagag
     3001 aacgctagaa cctctttaat gccgacaggc tgctcaaaaa gttccagctg cccccttcgg
     3061 ggacttccgg caattcgatt cttggcggtg ggtcgcaagg ccgacgaccg agaatccgcg
     3121 ctgctccgaa ggagcggata taatccgcct tccttacgga tggcgaagca acgccgcaga
     3181 tgggcttttt cagcagcctg ctaaggagca ccatggaata catgccgaat accgcggacg
     3241 atcaccgcgc catgttggcc accattgggg tggcgcggtt cgaggacttg ctgacggaaa
     3301 ttcccgcgcg cgcgcgcctg aatcgcccgc tgacgctgcc gcccgcgctc agcgaattgg
     3361 aggtgcgccg cgagatcggc gggctgctcg accgcaacgc ccacgccgac cgctcgccct
     3421 gttttctggg cgcgggcgcg tacgaccacg ccattccgtc gatcgtgccg tttctcacct
     3481 cgcggtccga gttctcgacc tcgtacaccc cctaccaggc cgagatgagc cagggcatgt
     3541 tgcagaccat cttcgagttc cagaccatgg tctgcgaact gaccggcatg gacgtggcca
     3601 acgcctcgat gtacgacggc gcgtccgcgc tcgccgaggc cgcgctcatg gcctgccgtg
     3661 tcaccaaacg ctcccgcatc gtggcggcgg gcacgatcca cccgcaccac cgcgccgtgc
     3721 tcgccacgta cctctccggc atctcgcatc cggtccacac ggtgcggatc gtggacggcc
     3781 gcgcggacct ggacgatctt cgccggtcgt tgaccgacga caccgcggcg gtgctggtcc
     3841 agcaccccaa cttcatgggc aacctggaag acgtggccgc gatcgcggag gccgcgcacg
     3901 gccggggcgc gctcgcggtc gcgtcggttg atccgctctc gctcggtttg ctggccccgc
     3961 ccggattctg gggcgcggac gtggccgtgg gcgaaggcca ggcgctcggc aaccccatgt
     4021 ccttcggcgg tccgttcgtg gggttcatgg cgaccaagaa ggatttcatg cggcagatcc
     4081 cgggccggat cgtgggcgcc accgtggaca ccaaggggcg accgggctac tgcctgacgc
     4141 tccaggcgcg cgagcagcac atccgtcgcg agcgcgcgac ctcgaatatc tgcaccaacc
     4201 aggcgctgat ggcgctcacc gcgtcgatct acctggccac gctcggcccc agcggtctgc
     4261 gcgaggtggg cgagcagtgc gtggccaaat cgcactacgc cgcggagcgg atcgcggccg
     4321 tgaaggggtt tgcccgcgcc gcggccgcgc cgttcttcaa ggagttcgtg gtccgaacgc
     4381 cgcgcccgcc cgcggagatc aacgccgccc tgttgaaagc cgggatcatc ggcggattgg
     4441 atctgggacg gttcgatacg gcatggacca accactggct ggtgtgcgtg accgaaaaac
     4501 gaacgcgtga agagatcgac cggctggtcg aggtcatagg gaacatataa ggcgttcaag
     4561 ggttgaaacc gttgaatctt gaacgttgaa cctttgaaca aattttttaa gggagatctg
     4621 atgcctgaac cgttactgtt cgagaagagc actactggcc gccggggata tgccttgccc
     4681 gccctggacg tgcccacgcc cgctgccgat acgatcccga cgggtgcgcg ccgggcgacg
     4741 ccccccgccc tgccgcaact cagcgaagtg gacgtgatcc gccattacac gcgtctctcg
     4801 cgcgagaact acggcgtgga cgcggggttt tatcccctgg ggtcctgcac catgaagtac
     4861 aaccccaaga tcaacgaaga aatggcccgt cagccggggg ctgcgttggc ccacccgctc
     4921 caaggtgaag cgctgtcgca gggcgcgctg cgcctgatgt gggagctcga acaagaattg
     4981 aaggaagtcg ccggcgtgga ccgcgtctcg ctgcaacccg cggccggcgc gcagggcgag
     5041 ttcgcgggca tcttgatgat ccgcgcgtac cacgcggcca agggctcgcc gcgccgccgc
     5101 gtgctgatcc cggattcggc gcacggcacc aatcccgcgt ccgcggccct ggccggctac
     5161 gaggtccaga ccgtgcactc caacgccgag ggtctggtgg acgtgggcga cctggcgaag
     5221 ttgatcaacg aggaatgcgc cgcgctgatg ttgaccaacc ccaacacgtt gggactgttc
     5281 gagaaggaaa ttctcgagat cgcgcgcatc gtgcacgacg cgggcgcgct gctgtacatg
     5341 gacggcgcga acctcaacgc gctcttgggc ctggtgcgcc ccggcgacat gggctttgac
     5401 gtggtgcact tcaacctgca caagacgttt tccacgcctc acggcggcgg cggtccgggc
     5461 gcgggaccga tcggcgtgaa atcgccgctc atcccctatc tgccgacgcc gaccgtggag
     5521 cgcgacggcg accggttcta tttcgacgag aaccggccgc aatccatcgg ccgcttgcac
     5581 ggcttttacg gcaacttcgg catcctagtg cgcgcgtaca cctatctgcg gcggctgggc
     5641 gcggcgggac tcgcggacat cagccgtcac gccatcatca acgccaacta cgtgcgcgcc
     5701 cgcctcggcg gcgcgtacca ggtcgcgtac gaccgcgcgt gcatgcacga atgcgtcctc
     5761 tcggccaaac gccaacgctc caagggcgtg cacgcctggg acatcgccaa acgcctgatc
     5821 gactacggca tctacccgcc caccatcaac ttcccgatgg tggtggaaga ggccatcatg
     5881 gtcgagccgc ccgagagcga atcgcggcag accatcgacg cgttctgcga cgccatgttg
     5941 gccatcgcca aggagtgcga agagcaaccc gatctggtca agggcgcccc ccacaccacg
     6001 ccgcgctcgc gcctggacga agtgttggcg gcgaggaagc cggtggtacg gtggtccccc
     6061 cacccctccc tccccctcaa ggggggaggg taagggaggg ggtgccccct atgctctctc
     6121 acgatattct gattatcggc ggcggcttgg ccggcatgcg cgcggccatc gaggtgccgt
     6181 ccgacctgga cgtggccctg atctccaagg tgcatccgtt gcgcagtcac tcggtggccg
     6241 cgcagggcgg catcaacgcc gcgatcgacc cgcaggactc gtgggagtcg cacacgttcg
     6301 aca
//
LOCUS       DBNGTT010000409         2735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_97381, whole genome shotgun sequence.
ACCESSION   DBNGTT010000409 DBNGTT010000000
VERSION     DBNGTT010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_97381"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="AC16BC_09235"
     CDS             complement(<1..589)
                     /locus_tag="AC16BC_09235"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3241842.1"
                     /translation="MIPKSIRAVPWTADAPAVIAHRGASAVAPENTLAAFRLAVRAGA
                     PVVEFDVHQTRDHRLVVVHDASLQRTTGVRRAVEHLTAREIIGLDAGSWFGPEHRGAR
                     IPTLDQVLGALTGRAALNIELKAGTRRLYPGLESRVLALVRRSGWRERVLVSSFHVRY
                     LQRLRRLDATIAIGVLIHPWSLRGALSRAKRLAAAS"
     gene            628..936
                     /locus_tag="AC16BC_09240"
     CDS             628..936
                     /locus_tag="AC16BC_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR3241843.1"
                     /translation="MTQPNQPNPPLQIQVELDDATAQGIYTNLALVAHTETEFLFDFL
                     FVQPQQPKAKVRARIISSPTHTKRFFLALRENITRYEVRFGEIKVPDDSPGARDLPHE
                     "
     gene            933..2057
                     /locus_tag="AC16BC_09245"
     CDS             933..2057
                     /locus_tag="AC16BC_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HIR3241844.1"
                     /translation="MIPRQRGQAVAWTLLAGWLLATPTFAADPLPRPVSLRGTLSSDG
                     ASASIRITVANPGERTSAPGTLEVYLSADGLVDPGDARIARRTLTPLAPDARIDIDLT
                     PPVPPQSPGRYYLVARVAPLDDQAVSPASDALWGAPLAIGPDLTIEELQTTPGRQGVV
                     LSGRVANRGTQAAVSGSLGAFWTARDGASVGHAPESASFNGLPPGGIAPFEWTATAGN
                     LPPGEYGIDVEVDPDHLIAESDDANNRSHGGGSFATGPDLAIAELSARQDGDTIVVHD
                     AAVNHGNAPSGSCGVLFVLSRNGIWDAGDVSIGYRIVPALDPDATSAAQTRFELPKER
                     LPTGRYFLLGKVDASNHVAESREANNLTLAAAPVDLRLQP"
     gene            complement(2072..>2735)
                     /locus_tag="AC16BC_09250"
     CDS             complement(2072..>2735)
                     /locus_tag="AC16BC_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3241845.1"
                     /translation="GDFFSTEFAGYFAERGLACIRFRSQGDLGRLYGDPASLSFFRDL
                     LRARVIDTRRVLDWLSQRQEIDVNRVALVGFSHGAIVGSLVTAVDTRIKASALVLGGG
                     DLAGIIRDSREGALARIREGVMADEGLTLDGFYDTAAATLEDVDPLTYAPLVSPRTVL
                     MINARFDWVIRRPYTEALWRALGRPELVWLPAGHYTAGLFTSYARHKILDHITSVFHP
                     GS"
BASE COUNT          490 a          992 c          860 g          393 t
ORIGIN      
        1 tcgacgccgc ggcgagccgt ttggcccggg acaacgcccc acggagcgac cagggatgga
       61 tcaggacccc gatggcgatc gtcgcatcca gccgccgcag ccgttgcaga taccgcacgt
      121 gaaacgacga aaccaggacg cgctcgcgcc agcccgagcg gcggaccagc gcgagcaccc
      181 gggattcaag gccggggtac aacctccgcg tgccggcttt gagctcgatg ttgagcgcgg
      241 cccgtcccgt gagcgcgccc agcacctgat ccaacgtggg aatccgcgcg ccccggtgtt
      301 cgggcccgaa ccacgatccc gcatccaagc cgatgatctc gcgagccgtc aaatgctcca
      361 ccgcccgacg tacgccggta gtccgttgca gcgacgcgtc atgcacgacg accagccgat
      421 gatcgcgggt ctggtgcacg tcgaattcga ccacgggcgc tcccgcccgc acggccaacc
      481 gaaaggcggc caacgtattt tcgggagcga cggcggacgc gccgcgatgc gcgataaccg
      541 ccggcgcatc ggccgtccac ggcacggcgc gaattgactt gggtatcatg ttccggtaca
      601 gtccatgccg ttttccgagg aattcccgtg acccagccca accagccgaa cccgccgctg
      661 caaattcaag tcgagctcga cgacgcgacg gcgcagggaa tatataccaa cctcgcgctg
      721 gtggcgcaca cggaaaccga gtttctattc gattttctct tcgtccagcc gcaacaaccc
      781 aaagccaagg tccgcgcgcg gatcatctcc agtcccaccc acaccaaacg gttctttctc
      841 gccttgcgcg agaatatcac gcggtacgaa gtgcgcttcg gcgaaatcaa ggtgccggac
      901 gacagccccg gcgcgcgcga ccttccccac gaatgatccc gcggcaacgg ggccaggcgg
      961 ttgcatggac cctgctcgcc ggatggctgc tcgcgacgcc gacgttcgcg gctgaccccc
     1021 tgccccgacc cgtgtccctg cgcgggacgt tgtcatccga tggcgcgtcc gcgtcgatcc
     1081 ggatcaccgt cgccaacccg ggagagcgga ccagcgcgcc gggcacgctg gaagtctacc
     1141 tctcggcgga cgggctggtc gatcccggcg acgcgcggat cgcccggcgc acgctgactc
     1201 cgcttgcgcc cgacgcccgg atcgacatcg acctgacgcc gccggtgccg ccccaatccc
     1261 ccgggcggta ctacctggtc gccagagtgg ctcccctcga cgatcaagcg gtctcgccag
     1321 cgtccgacgc tctctggggc gctccgttgg ccatcggccc cgatctgacc atcgaggagc
     1381 tgcaaaccac cccgggacgc cagggtgtcg tactgagcgg ccgagtcgcc aaccgcggca
     1441 cccaagccgc ggtctcggga tcgctcggcg cgttctggac cgctcgggac ggcgcctccg
     1501 tcgggcacgc cccggaatcc gcctcgttca acggccttcc gcccggcggc atcgccccgt
     1561 ttgaatggac ggcgaccgcc gggaacctgc ctccgggcga gtacgggatc gacgtggagg
     1621 ttgaccccga ccacctgatc gccgagtcgg acgacgcgaa caatcgatcg cacggcggcg
     1681 gctccttcgc gaccgggccg gacttggcga tcgcggagtt gtccgcgcga caagacggcg
     1741 ataccatcgt cgtgcatgac gccgcagtca accacggcaa cgccccatcg ggctcgtgcg
     1801 gcgtcctgtt cgtcctgtca cgaaacggca tctgggacgc cggcgacgtg tccatcgggt
     1861 accggatcgt gcccgcgctc gatcccgacg cgacctccgc ggcacagacg cgcttcgaac
     1921 tccccaaaga gcgtctcccc acgggccgct actttctgct gggaaaagtc gacgcgtcca
     1981 accacgtggc ggagagccga gaggccaaca acctcacgct ggccgccgcc ccggtggacc
     2041 tccgtctcca accctagccg cgtccgcacg gtcatgatcc gggatggaac acgctcgtga
     2101 tgtggtccag aatcttgtgc cgcgcgtagg acgtgaagag ccccgcggtg taatgaccgg
     2161 ccggcagcca gaccagctcc ggacgaccca acgcgcgcca cagcgcctcg gtatagggcc
     2221 gacggatgac ccagtcgaac cgcgcgttga tcatgagcac cgtgcggggc gaaaccagcg
     2281 gcgcataggt caacggatcc acgtcctcca gggtggcggc agccgtgtcg tagaaaccat
     2341 ccagggtgag gccttcatcg gccatcaccc cctcgcgaat gcgggccagc gccccctctc
     2401 gggagtcccg gatgatgccg gcgaggtcgc cgcccccgag cacgagggcc gaggccttga
     2461 tccgcgtatc aacggccgtg accaggctgc cgacgatggc tccatgcgaa aagcccacca
     2521 gcgcgacgcg gttgacgtcg atttcctggc gttgtgacag ccagtccagc acgcgccggg
     2581 tgtcgatgac tcgggccctg agcaggtcgc gaaagaacga gaggctggcc gggtccccgt
     2641 agagccgtcc aagatcgcct tggctgcgaa agcggataca cgccaacccg cgttcggcaa
     2701 aatacccggc gaactccgtg gagaaaaaat cgccc
//
LOCUS       DBNGTT010000410         1588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2396410, whole genome shotgun sequence.
ACCESSION   DBNGTT010000410 DBNGTT010000000
VERSION     DBNGTT010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2396410"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16BC_09255"
     CDS             complement(<1..184)
                     /locus_tag="AC16BC_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241846.1"
                     /translation="MAVNLFTINETGGAGAAGSEKFVPTFISMADPTGIGGTFTQGGI
                     YIPYMGTLTTCTSLIGT"
     gene            589..>1588
                     /locus_tag="AC16BC_09260"
     CDS             589..>1588
                     /locus_tag="AC16BC_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3241847.1"
                     /translation="MPLSSGHPPVTDPVDLSALRIDRPREHTPRRVPHPFFGWGILVA
                     VIVVGAWAAYSWTTSPIAVDVGAATTLSSNNASAMLHASGYVVAQRQADVASKATGRL
                     VNLYVGPGDRVRTGQVLAKIESADVAARLHQAQAGLAAAQAAVAQAQAAADEARLDFE
                     RKQRLRDANLVSGAEFDVASARHATAQAGVVSAEAQARAAEAAVRAAGVELDNTVIRA
                     PFDGTVLTKNADVGEIVAPFGSSATARAAVVRIADMRSLMVEADVSERHLDQVVKGSP
                     CEITLDAYPQLRYRGTVETIVPTADRAKATVLTKIRFADLDDRVLPEMSAKVAFFSA"
BASE COUNT          280 a          526 c          513 g          269 t
ORIGIN      
        1 tggtcccgat gagcgacgta caggtggtca acgtccccat gtacgggatg tagatcccgc
       61 cctgggtgaa cgtcccgccg atcccggtgg gatcggccat cgagatgaac gtcggcacga
      121 acttctcgct gccggcggca cccgctccgc ccgtttcatt gatggtgaaa aggttgacag
      181 ccatcgcagg cacggtgctc aacacgaagg cacacaccgc cagccccgac accatagatc
      241 tacagtgctt tcgcatcatg tgatcacccc cttccctgcg ataaggttac agggctttcg
      301 ccctggttca gccttgctct gaaccgcgtc cacgcatcag gaaacaccac tcccgcgccg
      361 ccttacggat acacggccgc gacaaccgac ttttcgagtg aaggcttatt ttaggagtag
      421 ttaaaacgtg aggtcaatat atttgtttcg accggtcaac actaattgca caggttcagg
      481 tacaggtaaa atcaccggtt ccccctttcc ccagcccacg ccgtacagcc cgtcggatct
      541 gaccggagat ctcgccatcg cgcgcccgac ccggtataat cggccggcat gccgctctcg
      601 tccggacacc cgcccgtcac cgaccccgtc gatctctcgg cgcttcgcat cgatcgtccg
      661 cgggaacaca ctcctcgccg ggtcccgcac ccgttcttcg ggtggggcat cctggtcgcg
      721 gtcatcgtgg tcggcgcatg ggccgcgtat tcatggacca cgtcgccgat cgcggtggac
      781 gtcggcgcgg ccaccacgct cagttcgaac aacgcgtcgg ccatgctgca cgccagcgga
      841 tacgtggtgg cgcagcgcca ggccgacgtg gcgtccaaag ccacggggcg gctcgtgaac
      901 ctgtacgtcg gacccggaga ccgggtccgc accgggcagg tgctggccaa gatcgaaagc
      961 gcggacgtcg ccgcgcggct gcatcaagcc caggccggtc tggcggcggc gcaggccgcg
     1021 gtcgcgcagg cgcaggccgc ggccgacgag gcgcgattgg acttcgagcg caaacaacga
     1081 ttgcgggacg cgaacctggt ttcgggcgcc gagttcgacg tggcgtcggc tcgacacgcc
     1141 acggcccagg ccggcgtggt ttccgcggaa gcgcaggcga gggcggccga ggccgcggtg
     1201 cgggccgcgg gggtggaact ggacaacacc gtgattcgcg cgccgttcga cggcacggtg
     1261 ttgaccaaaa acgcggacgt gggcgaaatc gtggcgccgt tcgggtcgtc ggccacggcg
     1321 cgcgccgcgg tggtgcggat cgcggatatg cgctcgctga tggtcgaagc cgacgtgtcg
     1381 gaacgccacc tggaccaggt cgtcaagggc tcgccgtgcg agatcacgct ggatgcctat
     1441 ccccagctac gctatcgcgg aacggtcgag acgatcgtgc ccaccgcgga ccgggccaaa
     1501 gccacggtcc tgaccaagat ccggttcgct gatttggatg atcgcgtgct gccggaaatg
     1561 agcgccaagg tggcgttttt ttccgcca
//
LOCUS       DBNGTT010000411         2549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1374961, whole genome shotgun sequence.
ACCESSION   DBNGTT010000411 DBNGTT010000000
VERSION     DBNGTT010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1374961"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="AC16BC_09265"
     CDS             <1..661
                     /locus_tag="AC16BC_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3241848.1"
                     /translation="LRLDVDPEFDLFRRLDRGELPPAFSQLFGAPRVTVVVPASAPAD
                     LLDGYQRLAETLTASSEHNEIVLDETLERLPEDRAVWVLGWENRFRPAISQAAAGLPV
                     TIGDSTARIQDTNLTRSGHAVVLSAHQPGNPEHGLGWIGCDRVAAMPGLARKLPHYGK
                     YGYLGFEGDEPTNVVKGEWPVTHSPMSVLVKQPDGSVRDVPRARLAARRGLAQIAGVS
                     P"
     gene            complement(872..1354)
                     /locus_tag="AC16BC_09270"
     CDS             complement(872..1354)
                     /locus_tag="AC16BC_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3241849.1"
                     /translation="MPKAAYQLLVVFVVFSAAVYLLLMIGPEESATKAGERDITLPRA
                     PAEAKAVANPIPPSDQVLVEGERIYQTKGTCFTCHGTTGRGDGPAGLELNPRPRNFTN
                     PRFHELRTDGEMFWVIRNGSPGTRMFSYSPSIITEDEAWKVIHYLRTLPTRAGTQTSG
                     "
     gene            complement(1358..1753)
                     /locus_tag="AC16BC_09275"
     CDS             complement(1358..1753)
                     /locus_tag="AC16BC_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241850.1"
                     /translation="MAHVLPTDKLYSGLTWELYKEHVKKFSHAAVKGMPTEQEMKEDL
                     AALVEAATVKVLAQERVGMVVTFHWMDTFPALEVCDITDVKGFLTQRYGGGKFKLNLY
                     HGINFLSTKNWETEGPPRWKDMIRGPEEG"
     gene            complement(1753..2088)
                     /locus_tag="AC16BC_09280"
     CDS             complement(1753..2088)
                     /locus_tag="AC16BC_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241851.1"
                     /translation="MSTTNELTAKQATSPSMAEQQAVEERLAEQESRLRDNQTELDHL
                     RRELDSLRAAVERLTARSVATCKKCGTTYDMFSHHYSIGLFDNIVYVKCPDCQTPMPV
                     DPRLGVRAE"
BASE COUNT          435 a          776 c          840 g          498 t
ORIGIN      
        1 gctgcgtttg gacgtggatc cggagttcga cttgtttcgg cgattggatc gcggggaact
       61 gcccccggcg ttctcccagc tcttcggcgc gccgcgggtc acggtggtcg taccggcctc
      121 ggcgcccgcc gacctgctgg atgggtatca gcgactcgcc gagacattga ccgcttcgtc
      181 ggagcacaac gagatcgtgc tcgacgaaac gcttgaacgc ttgccggaag accgcgcggt
      241 gtgggtgttg ggttgggaga atcggttccg acccgcgatc agtcaagccg ccgccggtct
      301 gccggtgaca atcggcgact cgaccgcgcg gattcaggac acgaacctca ctcggagcgg
      361 tcacgcggtc gtgttgtccg cgcaccagcc cgggaacccg gaacacgggc tgggctggat
      421 cgggtgcgac cgggttgcgg caatgccggg gctcgcgcga aagctcccgc actacggcaa
      481 atacggctac ctcgggtttg aaggcgacga gccgaccaac gtggtcaagg gcgaatggcc
      541 cgtgacgcac tcaccgatgt cggtgctcgt caagcagccc gacggctcag tgcgagacgt
      601 gccccgcgcc cggctggccg cccgtcgggg cttggcccag atagcagggg tgtcacctta
      661 gcgggatgct gtaaaactct agaaagccct ccggctgctc aaaaagtccc agctgcaagg
      721 ccgcagagag gaggaaaccg gagcgtactg gttcgtacgt gaggatttcc gacgagcgag
      781 aatctgtgcc gcgccgaagg tgtggggtgg gatcgactgc ttccttacag atggcgaagc
      841 aacgcagtag atgggctttt tcagcagccg gttagccgga cgtctgcgtg ccggctcgcg
      901 tcggcaacgt ccgcaagtag tggatcacct tccacgcttc gtcttccgtg atgatcgacg
      961 gcgaatacga gaacatccgc gtgcccgggc tgccgtttcg gatcacccag aacatttcgc
     1021 cgtccgttcg cagttcgtgg aatcgcgggt tggtgaagtt gcggggacgc gggttgagtt
     1081 caagtccggc gggtccgtcg ccgcggccgg tggtgccgtg gcaggtaaaa caggtgcctt
     1141 tggtctggta gatccgctcg ccttccacga ggacctggtc ggagggcggg atcgggttgg
     1201 ccacggcctt ggcttccgcc ggggcgcgcg gaagcgtgat gtcccgctcg ccggccttgg
     1261 tggcggattc ttccggtccg atcattaaga ggagatagac cgccgcggag aacacgacaa
     1321 acaccacgag caactggtac gcggctttgg gcacgggcta cccttcttcc ggaccgcgga
     1381 tcatgtcctt ccatcgcgga gggccttccg tctcccagtt cttggtcgaa aggaagttga
     1441 tgccgtggta caagttcaat ttgaatttgc cgccgccgta ccgctgggtg aggaatccct
     1501 tgacgtcggt gatgtcgcag acctccaacg cggggaacgt gtccatccag tggaaggtga
     1561 ccaccatgcc cacgcgttcc tgggccagga ccttgaccgt ggccgcttcc acgagcgcgg
     1621 ccaggtcttc cttcatctcc tgttcggtgg gcatgccctt gaccgcggca tgagagaatt
     1681 tcttgacgtg ctccttataa agttcccagg tcaggcccga atagagcttg tcggtcggca
     1741 gcacgtgagc cattactccg ctcgaactcc cagccgggga tcgaccggca tcggggtctg
     1801 acagtctgga cacttcacgt agacgatatt gtcgaacagc ccgatgctat aatggtggct
     1861 gaacatgtcg tacgtcgtcc cgcatttctt gcacgtggcg acgctcctgg ccgtgagtcg
     1921 ctccaccgcc gcgcggagcg agtccagctc ccggcgcagg tggtccagtt cagtctgatt
     1981 gtcgcggagc cgggattcct gctcggcgag acgttcttca accgcttgct gctcggccat
     2041 cgacggcgag gtggcttgtt tcgcggtgag ttcatttgtg gtggacatgg cgctcccaat
     2101 cgacaagtgg tccgagacga cgcgtgctcg aaccgggcga tcatacacat ttttcatccc
     2161 gggaacaagc cgaccgctct cctccggtcc gcagcccgga gaaatcggtc ttctccgggc
     2221 cgtcgtgttg tgttatgctt cggcgaccgc gcggtccatc cgggttcccg atgttgagcg
     2281 tcgcgcgcgg tcgaacgtct tcgacgtgca gtgaggcgcc cgcgcacgtt actcaaacag
     2341 cccgctgtgg gcgatagagt cggcgcaacg cgacgccacg cacgtgctca ggcaccatca
     2401 gggaggaacg cggccacggt cacgcatggc ttccgctgtc ctatgcgggg cgccgcgctc
     2461 gtgaacacac attcactcga gggggacggt atgattacgg agaaacgggc gtctcgccgc
     2521 gtgacgctca tgggcacgac gctcgtcag
//
LOCUS       DBNGTT010000412         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2781295, whole genome shotgun sequence.
ACCESSION   DBNGTT010000412 DBNGTT010000000
VERSION     DBNGTT010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2781295"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="AC16BC_09285"
     CDS             <1..232
                     /locus_tag="AC16BC_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3241852.1"
                     /translation="LKFVRAYSESGGRDLYGFMMKEFERPLLEFALAQTSGNQRRAAD
                     LLGMSRNTLRKRMQLCGIAQKSSTPRVRRRPR"
     gene            complement(252..770)
                     /locus_tag="AC16BC_09290"
     CDS             complement(252..770)
                     /locus_tag="AC16BC_09290"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241853.1"
                     /translation="MTVVVARGIVAAALTLTALALGGCGTLQAHAPSAPAEPDASAPP
                     QEVVAYHLVEDGRRQLDAGQAAAAVATFQKALALAPSSPHANLALAEAKIRQGEFRAA
                     LIYANRVLRLTSDRSDWRWRVALVRGQAFEGTGDAASAQAEFRRVLEQDPENEDAKSG
                     LSRLETGTGLPN"
     gene            complement(767..>1978)
                     /locus_tag="AC16BC_09295"
     CDS             complement(767..>1978)
                     /locus_tag="AC16BC_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3241854.1"
                     /translation="DELERLYSRDALASEGLRIFTSLDVEAQRAAEEALRAGLVALDR
                     RAPKASETLQGGAIVLQPQTGYVRALVGGRDYAASQFNRMTQARRQPGSLFKPIAYLA
                     ALKKNGETGAPRYTPVSVIKDERLTIATPSGPWTPQNYDKRYHGPVTLRTALEYSFNA
                     AAVQVGQDIGVDHVVETARLLGLTTPLQAVPSLVLGTSEVSPLELAGAYAVFANFGIR
                     TEPLVIKAVVDRDGQVVSRKEIPVEQAIAPEEAYLMTALLQGVIERGTGRGVRAMGFT
                     KPAAGKTGTTSDFRDAWFVGYTPETLALVWVGFDHNQPLNLTGSEAALPIWTDIMKRV
                     TVTEPERDFVPPPGIVVRQIDPATGLLSARQCPDGITEAFLAGTEPTATCADPEQRGP
                     SLLRWLRRVLG"
BASE COUNT          316 a          702 c          630 g          330 t
ORIGIN      
        1 gctgaagttt gtcagggcgt acagcgagag cggcggacgg gatctgtatg gcttcatgat
       61 gaaggaattc gagcggccct tgctcgaatt cgcgctcgct caaacgagcg ggaatcagcg
      121 acgcgccgcg gacctgctgg ggatgagccg caacacgctg cgaaaacgca tgcagctctg
      181 cggcatcgcg cagaaatcct caacgcccag ggttcgtcgc cgcccccgtt gagcaagggg
      241 gcatgccggc ctcagttggg gaggcccgtc ccggtttcga ggcgcgagag tccggatttc
      301 gcgtcttcgt tctcaggatc ctgttcgagc acccgccgga attccgcctg cgcgctcgcc
      361 gcatcgccgg tgccctcgaa tgcctgtccg cgcaccaggg ccacccgcca tcgccagtcc
      421 gaccggtccg aggtcaaccg cagcacccga ttcgcgtata tgagcgcggc ccggaattcg
      481 ccttggcgga tcttggcttc ggcgagcgcc aggttcgcgt gggggctgga gggcgccagg
      541 gccagcgcct tctgaaacgt ggccaccgcc gccgcggcct gaccggcatc gagctgcctg
      601 cggccgtctt ccaccaagtg atacgccaca acctcctgcg ggggagcgct cgcgtccggc
      661 tcggccggtg ctgacggcgc gtgcgcctgc aacgtcccgc acccgccgag agccagcgcg
      721 gtcaacgtga gcgccgccgc gacgatgcca cgcgccacga cgaccgtcat cccaacaccc
      781 gccggagcca ccgtaaaaga ctggggcctc gctgttcggg atcggcgcag gtggcggtcg
      841 gttccgtgcc cgccaaaaac gcctcggtga tcccgtcggg gcactgccgc gcgctgagca
      901 gtccggtggc ggggtcgatt tgccgcacca cgatcccggg aggcgggaca aaatcccgct
      961 caggctccgt gaccgtgacc cgtttcatga tatcggtcca gatcggcagc gcggcctcgg
     1021 aacccgtcag attgagcggt tggttgtggt caaaccccac ccacaccagg gcgagggttt
     1081 ccggcgtgta gccgacgaac cacgcatccc ggaagtcgct ggtggtgccg gtcttgccgg
     1141 ccgcgggttt cgtgaagccc atggcccgca cgccgcgccc ggtccctcgt tcaatcacgc
     1201 cctgcaggag cgcggtcatg agatacgcct cttccggggc gatcgcctgt tcaaccggaa
     1261 tttctttccg gctcacgact tgtccgtcgc gatccaccac ggccttgatg accagcggct
     1321 ccgtccgaat gccgaagttc gcgaacaccg cgtacgcccc cgcaagctcc agcgggctca
     1381 cctccgacgt ccccaacacg agcgacggca ccgcctgcaa gggcgtcgtc agtcccaaca
     1441 gtcgcgcggt ttcaaccacg tgatcgaccc cgatgtcctg gccgacctgg accgccgcgg
     1501 cattgaacga atactcgagc gcggtacgaa gcgtcaccgg accgtggtat cgcttgtcat
     1561 aattctgcgg cgtccacggg ccggacggcg tggcgatcgt gagacgctcg tctttgatca
     1621 cgctcaccgg cgtataccgg ggcgcgccgg tctccccgtt tttcttcagc gcggccaggt
     1681 acgcgatcgg cttgaacagc gatcccggct gccggcgggc ctgcgtcatc cggttgaact
     1741 gcgacgccgc gtagtccctg cccccgacca gggcgcggac atacccggtc tggggctgca
     1801 atacgatcgc gccgccttgc agcgtttccg atgccttcgg cgctcgccgg tcgagcgcca
     1861 caagccccgc gcgaagcgct tcttccgccg cgcgctgggc ctccacgtct aaactggtaa
     1921 aaatccgcaa cccctcggac gccagcgcat cgcgcgagta gagccgttcg agttcgtc
//
LOCUS       DBNGTT010000413         5186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_992519, whole genome shotgun sequence.
ACCESSION   DBNGTT010000413 DBNGTT010000000
VERSION     DBNGTT010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_992519"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..171)
                     /gene="thiS"
                     /locus_tag="AC16BC_09300"
     CDS             complement(19..171)
                     /gene="thiS"
                     /locus_tag="AC16BC_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR3241855.1"
                     /translation="MLDLLKARNVEPQMVSVEHNLKILEKEEFATTPLRDGDSIELLY
                     YMGGGE"
     gene            500..943
                     /gene="mraZ"
                     /locus_tag="AC16BC_09305"
     CDS             500..943
                     /gene="mraZ"
                     /locus_tag="AC16BC_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR3241856.1"
                     /translation="MFFGQHQHTIDSKGRLSIPGKYREILAQRASGILVVTKDPEQCL
                     AIVPIEEWRQRAAKIQAIPNSVQAVRDYKRFLFSEAADCALDGQGRILIPPELRRYAG
                     LERDVMLVGVDGHMEAWSFARWEAKSAQVSRDLPKIVDVIAQYGV"
     gene            1128..2063
                     /gene="rsmH"
                     /locus_tag="AC16BC_09310"
     CDS             1128..2063
                     /gene="rsmH"
                     /locus_tag="AC16BC_09310"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR3241857.1"
                     /translation="MSAIHRPVMVREVVQWLACAPGRRMADLTVGLGGHASAILAACA
                     PDGLLVGLDRDEQALAVARTRLPADRVRLHHANARALPEVVAREGWGRVDGVLLDLGV
                     SSLQLDTPERGFSFQADGPLDMRMDRRNPLTAAVIVNEWPEDALADVIRRYGEERWAG
                     RIAAAIARARSLHPLTGTKELAGLVVDAIPAKFRAPHPHPATRTFQALRIAVNRELED
                     LAPTLEAARECLTVGGRLVVISFHSLEDRIVKQTFRNWEGACVCPPGLPVCGCGRRRL
                     ARVLTPRPIAPDARETRENPRARSAKLRAVERAAA"
     gene            2100..2471
                     /locus_tag="AC16BC_09315"
     CDS             2100..2471
                     /locus_tag="AC16BC_09315"
                     /inference="COORDINATES: protein motif:HMM:NF016860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR3241858.1"
                     /translation="MIRRLTGWCRSPRGAWAIWSAVIAFSVVVLWHHHRTIQLGYETE
                     RLQAEKARLDRLQRQLLIERESLASLERIEPLARAMGLTHVPPRDALVVRLPERASDE
                     ERTLIAAVGRGMLPTDAHAAP"
     gene            2458..4197
                     /locus_tag="AC16BC_09320"
     CDS             2458..4197
                     /locus_tag="AC16BC_09320"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR3241859.1"
                     /translation="MRHPEDADQAARRRAFWVLGAFAVLYAALALRVVYLHVVRGPEL
                     AHRAERQHEKSVAVEAERGTIYDRNGRVLATDLTVPSLYAAPPLIDNPESIARELAAM
                     LRVDAKPLARRLAEDRGFIWLERRVDPAVARAVEELGAEGVRVVPEPSRVYPRKTLLS
                     HVLGFAGTDHVGLEGVERFYDRDLRGETGRIIYERDGAGKRLFPKGLEYVAPSRGHDL
                     VLTIDEGIRHISERELDAAMSETGAESGTVVVMDPWDGGVLALAVRPEFNPNEPNVSR
                     PEAWRNRAITDTFEPGSTFKIVTAAAALDAGLVRPDEIIDCEQGRWPITGGVLHDHEP
                     LGRVPFSEVVAKSSNIGTAKIAQRLGPERLRRAITDFGFGRKTGIDLGGEAAGRVAPL
                     ARWSKRTAVTMAIGQELMVTTMQLAVAYAAVANGGRLVTPHVVAHVRGADGLDAAGAA
                     SPGNRVMSERTAGVMARLLEGVVGEDGTGALAVVEGIRVAGKTGTAQQVDPETGRYSA
                     DRETASFIGFAPSTRPAVVIAVVLDNPSGRGWGGRAAAPVFSRVAAGAVHYLSTPAAT
                     PAPQDGVLRVALR"
     gene            4194..>5186
                     /locus_tag="AC16BC_09325"
     CDS             4194..>5186
                     /locus_tag="AC16BC_09325"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR3241860.1"
                     /translation="MTPSRRLGDLLRGIAGVTVSGGADHDIARVTADSRRVVPGSLFV
                     ALGGTRMDGHRFLADAVARGAVAIAGAEAETIRRVEGPANLARVVVPGGAAWFGVVTA
                     RWWGDPSHRLGMVGVTGTNGKTTTTHVIRSVYEAAGRRTGLIGTVAYAIGEEIRPASH
                     TTPDAEALHALLAEMAARGMTDVVMEVSSHALAQDRIAGCAFDVGVFTNLTQDHLDFH
                     GSMDAYAAAKRRLFESLGRDNPKTRPRLAVINRDDPRWSEMARASREPVWTFGLTPDA
                     DVRPESVSSTVEGIRCRVRTPRGGLDLTSPLLGSYNLSNLLAATATAVGQDVPLD"
BASE COUNT          763 a         1577 c         1984 g          862 t
ORIGIN      
        1 cgacgtccgg cgccgcgttc actcgccccc gcccatatag tacaggagtt cgatcgagtc
       61 gccgtctcgg agcggggtgg tcgcgaattc ctctttttcg aggattttga ggttgtgttc
      121 caccgacacc atttgcggtt cgacgttgcg ggccttcagc aggtccagca ccgtggtctg
      181 cgggatgtcc tcggttttcc gttgactctg accttcatgg gggtccttgg ttcgcggtct
      241 cgtgagtggt cgtcaccggc gagccccgag ggtgcccgcc gcgcaccggg acaaccggcg
      301 catcactgta aaaagaactt catttgtagc aaatagggga aaaatgtgtc aaggcagcgc
      361 cgaactcgat ctgtggcaga accaaacgcg cagtccgcgt cctctccttg attttttcgc
      421 ttgatttcgc tgtggtgttt gcgttacgtt gccacccaca aagtggggta aagtgggata
      481 gggtagggga gcggaccacg tgttttttgg ccagcaccag cacaccatcg actctaaagg
      541 ccggctcagc attcccggga agtaccgcga gatcctggcg caacgggcgt cgggcatctt
      601 ggtggtcacc aaggacccgg aacagtgtct ggccatcgtg ccgatcgaag aatggcggca
      661 gcgggcggcc aagatccagg cgattcccaa ttcggtgcaa gcggtgcggg attataagcg
      721 ctttctgttc agcgaggcgg ccgactgcgc gctcgatgga caggggcgaa ttctcatccc
      781 gcccgagctg cgccggtacg cggggctgga gcgggacgtg atgttggtcg gcgtggatgg
      841 ccacatggag gcgtggtcgt tcgcgcgttg ggaggcgaag tccgcgcagg tgtcgcgcga
      901 tctgcccaag attgtggatg tgatcgccca atacggcgtg taggagggaa gctgtccatg
      961 accggccatc tgcggcgttg ccgctgcgcg tccgctcctc acgcacgaac tagtgcgctc
     1021 cggtgcgggc gctcgcggcg ccttgcatat ggcgcggtcc tgaacagctt ccatactccg
     1081 cggcggatac accagtttgt gaaaaggcgt gagcaggaga gctcatcgtg tcggcgattc
     1141 atcggccggt catggttcgc gaggttgtgc agtggctggc gtgcgcgccg gggcggcgca
     1201 tggcggattt gaccgtggga ctggggggac acgcgtctgc gattcttgcg gcctgcgccc
     1261 cggacggact gttggtgggg cttgatcgag acgagcaggc gttggcggtc gcgaggacgc
     1321 gactccccgc ggaccgcgtg cggctgcacc atgcgaacgc gcgcgcgttg ccggaggtag
     1381 tggcgaggga gggctggggc cgcgtggacg gtgtgctgct cgacctcggg gtgtcgtcgc
     1441 tgcaactgga tacgcccgaa cggggcttct cgtttcaagc cgacgggccg ctggacatgc
     1501 gcatggatcg gcgaaacccg ctgaccgccg ccgtgatcgt gaacgagtgg ccggaagacg
     1561 cgttggccga cgtcatccgg cggtacggcg aggagcggtg ggccgggaga atcgccgccg
     1621 ccatcgcgcg cgcgcggagt ctgcatccgc tcacgggcac gaaggaactg gccggtttgg
     1681 tggtggacgc gatccccgcg aagtttcgcg cgccgcatcc gcaccccgcg acccggacgt
     1741 tccaggcgtt gcggatcgcg gtgaaccggg aactggagga cctcgcgccc acgctggagg
     1801 ccgcgcgcga gtgtttgacc gtgggcgggc ggttggtggt gatttcgttt cactcgctgg
     1861 aagatcgcat cgtgaagcag acgttccgga actgggaagg cgcgtgcgtg tgtccgcccg
     1921 ggctgccggt gtgcgggtgc gggcgtcggc ggttggcccg ggtcttgacg ccgaggccga
     1981 tcgcgccgga tgcccgtgaa acgcgtgaga acccgcgcgc gcgaagcgcg aaactgcgcg
     2041 cggtggaacg cgccgccgcg taagcggacg ccggggagtc ggaacgaatt gaggcgcgga
     2101 tgatcaggcg attgacgggg tggtgccgca gcccgcgcgg cgcgtgggcg atctggagcg
     2161 cggtcatcgc gttcagcgtg gtggtgctct ggcaccacca tcgcaccatc cagttgggat
     2221 atgaaaccga acggcttcag gccgaaaaag cgcggctgga ccgcttgcag cgccaactgc
     2281 tcatcgagcg cgagagcctt gcgtcgctcg agcggatcga gccgctcgcc cgcgcgatgg
     2341 gcctgaccca cgtgccgccg cgcgacgcgt tggtggtccg cctcccggag cgagcctccg
     2401 acgaggagcg cacgttgatc gcggcggtgg gccgcgggat gcttccgacg gacgcgcatg
     2461 cggcaccctg aggacgcgga ccaggcggct cgtcggcgcg cgttctgggt gctgggcgcg
     2521 ttcgcggtgc tgtacgcggc gctcgccctg cgcgtggtgt acctgcacgt ggtccggggt
     2581 ccggagcttg cacatcgtgc ggagcgtcag cacgagaagt ccgtggcggt ggaggccgag
     2641 cgcggcacga tctacgaccg caacggccgc gtgttggcca cggatctgac cgtgccgtcc
     2701 ttgtacgcgg cgccgccgct catcgacaac cccgaatcga tcgcccggga gttggcggcg
     2761 atgctgcggg tcgacgccaa accgttggcg cggcgtcttg ccgaggaccg cgggttcatc
     2821 tggctggagc gacgcgttga tcctgcggtg gctcgcgccg tggaggagtt gggcgccgag
     2881 ggcgtgcgcg tggtcccgga gccgtcgcgg gtttatccgc gcaagacgtt attgagccac
     2941 gtgttgggat tcgccgggac cgatcacgtg gggttggagg gagtggagcg gttctacgac
     3001 cgggatctgc ggggggaaac gggccggatc atctatgagc gggacggcgc cggcaaacgg
     3061 ctgttcccca agggactgga gtacgtggcg ccgtcgcggg gccacgacct ggtcctgacc
     3121 attgacgaag gcattcgaca tatcagcgaa cgggaactcg acgcggccat gtccgagacc
     3181 ggcgcggaga gcgggacggt cgtggtgatg gacccctggg acggcggcgt gctcgcgctg
     3241 gccgtccgcc ccgagttcaa tccgaacgag cccaatgtgt cgcggccgga ggcatggcgc
     3301 aaccgcgcga tcacggacac gttcgagccg ggttcgacgt ttaagatcgt gaccgcagcc
     3361 gcggcgcttg atgccgggtt ggtgcggccg gatgagatca tcgactgcga gcaggggcgc
     3421 tggccgatca ccggcggcgt gctgcacgac cacgaaccgt tgggccgagt gccgttctcc
     3481 gaggtcgtcg ccaagtccag caacatcggg accgccaaga tcgcgcagcg attgggcccc
     3541 gaacgtctgc gtcgcgcgat cacggacttc gggttcggcc gcaagaccgg gatcgacctg
     3601 ggcggcgaag ccgccgggcg cgtggcgccg ctggcgcgct ggtccaaacg aacggccgtc
     3661 accatggcta tcggccagga actgatggtg accacgatgc agctcgcggt cgcgtacgcc
     3721 gcggtggcca acggcggacg cctggtcacg ccccacgtgg tggcgcacgt gcgcggagcg
     3781 gacgggctcg acgcggcggg cgcggcgtcg ccggggaatc gcgtgatgtc cgagcggacg
     3841 gcgggggtca tggcgcggct gctggaaggc gtggtcgggg aggacggaac gggcgcgttg
     3901 gcggtggtcg agggcatccg cgtggcgggc aagaccggga ccgcgcaaca agtcgatccc
     3961 gagaccggtc ggtactcggc cgatcgtgaa accgcgtcgt ttatcgggtt tgcgccctcg
     4021 actcgtcccg cggtggtgat cgcggtggtg ctggacaacc cgagcgggcg agggtggggc
     4081 ggccgcgccg cggcgccggt gttttcgcgc gtggccgcgg gcgcggtgca ctatctgagc
     4141 acgcctgccg cgaccccggc gccgcaggac ggcgtgctcc gcgtggcgtt gcggtgaccc
     4201 cgtcgcggcg tctgggcgac ctgttgcggg ggatcgccgg cgtgaccgtg tcgggcggcg
     4261 cggatcacga catcgcgcgg gtgaccgcgg actcgcgtcg cgtggtcccg ggatcgctgt
     4321 ttgtcgcgct ggggggcacg cgcatggacg gccatcggtt tctcgcggac gcggtcgcgc
     4381 gcggagccgt cgcgatcgcg ggggcggagg ccgagacgat ccgtcgggtg gagggccccg
     4441 cgaatctggc gcgggtcgtg gtgccgggcg gcgccgcgtg gttcggcgtg gtgacggcgc
     4501 gatggtgggg tgatccgtcg caccgactgg gcatggtcgg cgtgaccggc accaacggca
     4561 aaaccaccac cacgcacgtg attcggtcgg tgtatgaggc ggcgggccgg cgcaccgggc
     4621 tgatcggcac ggtggcctac gcgatcggcg aggagatccg ccccgcctcc cacaccacgc
     4681 ccgacgcgga ggcgctgcac gcgctgttgg cggagatggc ggcgcgcggc atgaccgatg
     4741 tggtgatgga ggtgtcgtcg cacgcgctgg cgcaggatcg aatcgccggc tgcgcgttcg
     4801 acgtgggggt gttcacgaac ttgacgcagg atcacttgga ttttcacggc agcatggacg
     4861 cgtacgccgc ggccaaacgg cggctgttcg agtcgctcgg gcgggacaac cccaagacga
     4921 ggccccgtct ggcggtcatc aaccgcgacg atccgcgctg gagcgagatg gcgcgcgcct
     4981 cccgcgaacc ggtgtggacg ttcggactca cgccggacgc ggacgtgcgc cccgaatccg
     5041 tgtcctcgac ggtggagggc attcggtgcc gggtgcggac tccgcgcggc ggtctcgatc
     5101 tgacctcgcc gttgctgggg tcgtataatt tgtcgaacct gttggccgcg accgcgaccg
     5161 cggtgggcca agacgtgccg ctggac
//
LOCUS       DBNGTT010000414         3713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2143219, whole genome shotgun sequence.
ACCESSION   DBNGTT010000414 DBNGTT010000000
VERSION     DBNGTT010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2143219"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..572
                     /locus_tag="AC16BC_09330"
     CDS             93..572
                     /locus_tag="AC16BC_09330"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR3241861.1"
                     /translation="MPHKKIAMEHDKRIALVAHDNKKQDLVEWAKYNRTLLAHHVVYA
                     TGTTGKILEQELGFKISRLQSGPLGGDQQIGAKIVDGEIDFLIFFWDPLEPMPHDPDV
                     KALLRMAVVWNIPIACNRASADFMISSPLMDGEYDRLVPDYESYRRRKIPSGGIGGS"
     gene            682..1179
                     /gene="rfaE2"
                     /locus_tag="AC16BC_09335"
     CDS             682..1179
                     /gene="rfaE2"
                     /locus_tag="AC16BC_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HIR3241862.1"
                     /translation="MTSKLKTVSELAQLAATARREGRRVVFTNGCFDLLHIGHTRYLQ
                     EARSLGDLLMVAINSDASVSALKGPERPLVPEQERAEVLAALGCVDYVTIFDAPDPAD
                     VIAAIQPDVLAKGGDWPVDKIVGREVVQARGGRVVSIPLVPGSSTTGLVRRIVERATT
                     RASGA"
     gene            1206..>3713
                     /locus_tag="AC16BC_09340"
     CDS             1206..>3713
                     /locus_tag="AC16BC_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3241863.1"
                     /translation="MTTPLINVPSTELRDALRADATRVHVAGLRPPAKAEILRGAATV
                     RPVLAVTASLDAADVLAGDVRFTAALARERVAVQVIPDWNPALPPPIDLEARRVAALA
                     ALLDRSAAITIAPIDALRAPLISPAAFRAHTRTLERGLTVSREDLSRVLAAAGYEMVS
                     TVTLPGEVAIRGGIIDAFSPLEDAPVRIELDGDEVASLRLVDPLTQRSQRYVERVVIG
                     PAPGFVHADADSTLASYLALDTVLAIDEPELLPDAPLDGRLPATDGHVPLTRVLTLHS
                     LAIAAPGGWRLLTAEHAAPERFGIGTARQSVAAALQRLDEVRRDFLAVVVCRSPEQVG
                     RFKTLAADHNVPAADLPSGGVADSLSAIPTPVFVTSGELSAGLVDSASRLLVVTEEEL
                     LGKERIARPTRRARLTPFLSSIDELRPGDLAVHSEYGIGRYEGLSRMAMAGTESEFLV
                     LRYLGGDKVYVPIDRLDLVQPYGGVEGKTPRMEALGGTGWVKTKQRVRRAIQRMAADL
                     LALYAEREVAKGHAFPPEGIEARDFAAGFEYEETPDQARAIAEVALDMEKPRPMDRLV
                     CGDVGYGKTEVALRAAFKAVMGHKQVAVLVPTTLLAQQHARTFAARFAPFPVRVEALS
                     RFRPQAEQREVLAGLADGRVDIVIGTHRLLQKDVVFDDLGLVIVDEEQRFGVRHKERF
                     KELRKNVHVLTLTATPIPRTLQMALGAVRDLSLIETAPADRLAIRTTLAPWNPDLVRD
                     AIRRELARGGQIFMVHNRVADIERVAEEIRHLAPEARIVVGHGQLSDHQLERVMETFL
                     AKEADILLSTTIIESGLDIPSANTILINRADRFGLAEL"
BASE COUNT          612 a         1257 c         1236 g          608 t
ORIGIN      
        1 gattgaggct ctcgggaagc ggaccaggcc ttccggcaac cggaatcgca gcgcgtaaca
       61 accccaagac ctcccccctt cagaggtttt atatgccgca caagaaaatc gcgatggagc
      121 acgacaagag aatcgccctg gtcgcgcacg acaacaagaa gcaggacttg gtggagtggg
      181 ccaagtacaa ccgcacgctc ctggcgcatc acgttgtgta cgcaaccggg acgaccggga
      241 agatcctgga acaggaatta ggttttaaga tttcgcggct ccaaagcggg ccgttgggcg
      301 gcgatcaaca gatcggcgcg aagatcgtgg acggcgagat cgactttctg atctttttct
      361 gggatccgct cgaacctatg ccgcacgacc ccgacgtcaa ggcgctcctg cgcatggccg
      421 tggtctggaa catccccatc gcgtgcaacc gcgcctcggc cgacttcatg atctcatcgc
      481 cgctcatgga cggcgagtac gatcggctgg tcccggatta cgagagttac cgtaggcgaa
      541 agatcccgag cgggggaatc ggcggttcgt aatagttttg ccggaaaggg accttccact
      601 cgggtcctct tcgaggaccg acccgccgtt cttgcaaccc cgctgctgaa agggttacac
      661 ttttccgcaa cgactcgttt gatgacgtcc aagctcaaga cggtttctga actcgcccaa
      721 ctcgcggcaa ctgcccgccg ggaaggccgc cgcgtggtct tcaccaacgg ttgcttcgac
      781 ctcctgcaca tcggccacac gcggtacttg caggaagcgc ggtccctcgg ggatttgttg
      841 atggtcgcga tcaacagcga cgcgtcggtg tccgcgctca agggaccgga gcgaccgctg
      901 gtgccggaac aggaacgggc cgaagtcttg gccgcgctcg ggtgcgtgga ctacgtcacg
      961 attttcgatg cgcccgaccc cgcggatgtg atcgcggcga tccagcccga cgtcttggcc
     1021 aagggcggcg actggcccgt ggacaaaatc gtggggcgcg aggtggtgca ggcgcgcggc
     1081 ggccgcgtgg tgtccattcc cctcgtgcca ggctcgtcga ccaccggcct cgtgcgccgc
     1141 attgttgaac gcgccaccac ccgcgcatcc ggggcctgac cggggtgccg accactcacg
     1201 agtccttgac cacccctctg atcaacgtgc cgtcgaccga gttgcgcgac gcgttgcggg
     1261 ccgacgcgac gcgcgtgcac gtcgccgggc ttcgcccgcc ggccaaagcc gagatcctgc
     1321 gaggggccgc aacggttcgt cccgtgttgg ccgtgaccgc gtcgttggac gcggctgacg
     1381 tcctggcggg ggacgtccgg ttcacggccg cgctggccag ggaacgcgtc gcggtccagg
     1441 tgatcccgga ttggaacccc gcgctgcccc cgccgatcga cttggaagcc cgtcgagtcg
     1501 cggcgctggc ggcgctgctc gaccggtccg ccgccatcac cattgcgccg atcgacgcgc
     1561 tccgggcccc gctgatctca cccgccgcgt ttcgcgcgca cacgcggacg ctcgaacgag
     1621 gcctgaccgt ctcgcgtgag gacttgtccc gcgttctggc ggcggccggg tatgagatgg
     1681 tttccaccgt gaccttaccg ggcgaggtgg cgatccgtgg aggcattatc gacgccttct
     1741 ccccgctcga agacgcgccg gtccggattg aactggacgg cgacgaggtc gcctcgctgc
     1801 gactggtcga tcccctcacc caacgatcac agcggtacgt ggaacgcgtg gtcatcgggc
     1861 cggcgccggg ctttgtgcac gcggacgcgg actcgacact cgcctcgtac ctcgcgctcg
     1921 atacggtgtt ggcgatcgac gaacccgagt tgctgcccga cgcgccgctc gacggccgac
     1981 tgcccgcgac ggacggccat gtgccgttga cgcgggtcct gacgctgcac tcgctggcca
     2041 tcgccgcccc gggaggctgg cggttgctga cggcggagca cgcggcgccg gaacgattcg
     2101 gcatcggcac cgcgcgtcaa tcagtggctg cggcgctcca gcgcctggac gaggtgcgcc
     2161 gggacttcct cgccgtggtc gtttgccgca gccccgagca ggtcggccga ttcaagacgc
     2221 tggcggccga ccacaacgtg ccggccgctg atctcccctc cgggggcgtc gcggacagcc
     2281 tgtccgcgat cccgactccc gtgttcgtca catccggaga gctctcggcc ggcctcgtcg
     2341 actccgcgtc gcggctcctc gtggtgacgg aagaggagtt gctcggcaaa gaacggatcg
     2401 cccgccccac cagacgggcg cgactgaccc cgtttttgtc gtcgatcgat gaattgcggc
     2461 ccggcgacct ggccgtgcac agcgagtacg ggatcggccg ttacgaaggc ctctcgcgca
     2521 tggccatggc cggcaccgag agcgagttcc tcgtcctgcg ctatctgggc ggcgacaagg
     2581 tctacgtccc catcgaccgc ttggacctgg tgcagccgta cggcggcgtg gagggcaaga
     2641 ccccgcgcat ggaggcatta ggcggcacgg gatgggtcaa gaccaaacag cgcgtgcggc
     2701 gcgcgatcca gcgcatggcc gcggacttgc tcgcgctcta tgcggaacgc gaagtggcca
     2761 agggccacgc gttcccgccc gaagggatcg aagcgcgcga ctttgccgcg ggctttgaat
     2821 acgaagagac gcccgaccag gcgcgcgcga tcgcggaagt ggcgctcgac atggagaagc
     2881 cgcggcccat ggaccgcctc gtgtgcggcg acgtgggcta cggcaagacc gaggtcgcgt
     2941 tgcgcgccgc gttcaaagcc gtgatgggcc acaagcaggt ggcggtgctg gtgccgacca
     3001 cgctcctggc ccagcaacac gcccgtacgt ttgccgcgcg ctttgcgccg ttcccggtcc
     3061 gggtcgaagc gctcagccgg ttccgtccgc aggccgagca gcgcgaggtg ctggccggac
     3121 tcgcggacgg ccgcgtggac atcgtcatcg gcacgcaccg attgctccag aaggacgtgg
     3181 tgtttgacga cctcggcctg gtcatcgtgg acgaagagca gcgattcggc gtgcgccaca
     3241 aggagcggtt caaggaactc cgcaagaatg tccacgtgct cacgctgacc gccacgccca
     3301 ttccccgcac gctgcagatg gcgctgggcg cggtccgcga cctctcgttg atcgaaaccg
     3361 cgcccgccga ccgcctggcc attcgcacca cgttggcgcc gtggaatccg gacctcgtcc
     3421 gcgacgcgat tcggcgggag ctggcgcgcg gcggccagat tttcatggtc cataaccgcg
     3481 tggccgacat cgagcgggtc gcggaggaaa tccggcacct cgcgccggag gcccgcatcg
     3541 tggtggggca cggccagctg agcgaccatc aactcgaacg cgtgatggag acgttcctgg
     3601 ccaaggaagc ggacattctc ctctccacca cgatcatcga gtccggactc gacattccct
     3661 cggccaacac gatcttgatc aaccgcgcgg accggttcgg cctcgccgag ctg
//
LOCUS       DBNGTT010000415         4661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_739034, whole genome shotgun sequence.
ACCESSION   DBNGTT010000415 DBNGTT010000000
VERSION     DBNGTT010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_739034"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4661
                     /locus_tag="AC16BC_09345"
     CDS             <1..>4661
                     /locus_tag="AC16BC_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241864.1"
                     /translation="IVTPPQPTVTSVTLATVTYGITPSQSVTISGTNFVVVATVTVGT
                     LTGTTVAGSVATAAVPYVFVSSTTIRFWWPNTALAPGAYTVTVANPGAAGGLAGSLAD
                     GFTVAPPQPTVTSATPNAVTYGITPSQAITISGTNFVVGATVTLSSPTTATTLSGPTV
                     AGSLATATTPYVFVSATRLSVYWSSTSLAPGSYAILVTNPSNAGGLSAQAPDLLTVAG
                     AQPVVSTVTPTQVTYGTQASLAITISGSGFLNGAAIAVSGPSPSTTVLLSGTTQTGSV
                     ATASIPYVFVSATRLSFYWTNTSLPLGRYTITVTNPAASGGLASAPLVDGFEVAAPQP
                     VVSGTSPNQVTYDITPSRAITITGANFVDGAVITVGTPSASGVCALPTCASGTTVTGT
                     AATASTPFVFVRSSQLSFYWDELGWLPGRSYDVRVVNPAVAGSLGHTLAGAFTVVAPQ
                     PQITSITPSSGEYNVTPSTSVRIVGSNFYAPSGTDVLSVTVGSLPYTCSVGTAPATAT
                     TECVYIDSRNIEFYWTNIHNNPRVLPPGDHAVRVTNPVYAGAASSNAVTFRVNAPEPR
                     IETIRPLAVAAGISASRAIDVVGVDDAANYVGFLAGASLRVEDSVNPSQSLNCTAQAV
                     SGVTVPTTANPCIYVGPGSLRFYWDTDRSVDPVSPLLGPGSYSVNVTNPANRGGLPAP
                     TPGAFRVVAAPELISVEPAAGVGAGARSAVLTLATAGFPAGSTVKIGTAEPDPSDATA
                     PPYVQVQDPQVTMRSAFRAFAAGDGGTVVIDVADDPAQTTNRLLAVTITSQDGRYRFT
                     CPAPSGTGACASGLIIAPAPAITAITPALAVGASGRTVTITGSRFVAGGTSVTLPAGI
                     SGTCSATSAAAIDCTAVAVASAVTPGSLSVIVINPDGGRATGTLTINQPPVISSLTPG
                     TGAAGSTVRVQITGSGFQTGLALASEAGVTVTVVSVASGTVVADFALAAGAADGYRSV
                     TLTNPDGGSTAAAQAFYIGTPPADAESILSTFGQRGQSAIQYARSVGSGWSGRFSGPA
                     LSAPPVWQVMKASPLRDERLLAVVDDQRGLTLHVWNGQAWGGALAATGSTGLTRPTQT
                     VDVAFEQQSGRGVAVYAINDTTLRYRVWDGTSWSAEAWVEDSVNGQPTSGRPLWVRLE
                     ARPGTNDLILAYEDVNDHVAAFVWDGTTRTWGDAIVLTTTAPGHESALVDVAFERLTG
                     RGLVAWAIAGDPAGRPRYRVWNGGWGAEGIAQLGAGAAANTLTALRVVGDHASDRVAL
                     ASSDGTAMVAQMWDGQQWIATGSSAPVSVTLELAVEGRGRPFDLAWEGSSGDLVIAYA
                     PRSVSGGMSRSWSSAGGWTGPVALPAPTPETVITTTTASSVGVSDASLVLVATAALPD
                     AGLIQIDTELIRYSSKTTNTAGTLATLTGVTRGFGGTAATAHAAGAPVQFVPVPAWFD
                     LAAARGTNGLALATLDTQGRVTLRRWTGAAWAAPIETQSQAAGLVSVFDPALARNALV
                     VGRGVAVTLAQHLAGPGPVISPPGTVADTTAPSTPTFGGGTPAATTVSLSWTAVGDDG
                     M"
BASE COUNT          712 a         1520 c         1659 g          770 t
ORIGIN      
        1 tcatcgtcac gccgccccaa cccacggtga cgtcggtgac gctcgccacc gtgacgtacg
       61 gaattacgcc gagtcagtcg gttacgattt caggaacgaa ctttgtcgtg gtcgcgaccg
      121 tgacggtcgg aaccttgacc gggaccacgg tggctggcag cgtggccacg gcggccgtgc
      181 cgtacgtgtt cgtgagcagc accacgatcc ggttttggtg gccgaacacg gcgttggccc
      241 ccggcgcgta caccgtgacg gtggcgaacc cgggcgcagc ggggggcttg gccgggagtc
      301 tggcggacgg attcacggtg gccccgcccc aacccacggt gacgtccgcg actccgaacg
      361 cggtgacgta cgggatcacg ccgagccaag ccatcacgat ttcgggaacg aactttgtcg
      421 tgggcgcgac cgtgacgctg agtagtccga ccaccgccac gacattgagc ggaccaacgg
      481 tggcggggtc gctggcaacc gcgacgacgc cgtatgtgtt cgtcagtgcc acccgcttga
      541 gcgtgtattg gtcgagcacg tcgttggctc cgggatcgta tgcgatcctg gtcacgaacc
      601 cctccaacgc cggcggactc tccgcccagg cgccggatct gctgacggtg gcgggggcac
      661 agcccgtggt gtccacggtg acgccgaccc aggtgacgta cggtacgcaa gccagtttgg
      721 cgatcacgat ttccgggtcc ggcttcctca acggggcggc cattgcggtc agtggtccgt
      781 ccccgtccac gacggttctg ttgtcgggaa ccacgcaaac aggttcggtg gcgaccgcgt
      841 cgattccgta tgtcttcgtc agcgccaccc ggctgagctt ttactggacc aacacgtcgc
      901 tcccgttggg ccgttacacc atcacggtga ccaatccggc ggcgtcgggc ggcctcgcgt
      961 cggcgccact ggttgacggc ttcgaggtcg cggccccgca gccggtggtg agcgggacct
     1021 ccccgaatca agtgacgtat gacatcacgc ccagccgggc gattacgatc accggcgcga
     1081 atttcgtgga cggtgcggtg atcacggtgg gcacgccgtc ggcctcaggg gtgtgtgcgc
     1141 ttcccacctg cgcaagcggc accaccgtga ccgggaccgc cgcaaccgcg tccactccgt
     1201 ttgtattcgt ccgatcgagt caattgagct tttactggga cgagctgggg tggttgcccg
     1261 ggcggtccta cgacgtgcgg gtggtgaatc cggccgtggc gggcagtctc ggccatacct
     1321 tggcaggggc gtttaccgtt gtggcgccgc agccgcagat taccagcatc accccgtcgt
     1381 ccggggagta caatgtcacc cccagcacca gcgtccgcat cgtgggttcg aacttctacg
     1441 ccccgagcgg caccgacgtc ctgtcggtca cggtgggatc attgccgtac acctgcagcg
     1501 tgggaaccgc tccggcgacg gcgaccaccg aatgcgtgta catcgactcg aggaatatcg
     1561 agttttattg gacgaacatt cataacaatc cccgcgtgct tccgcccggt gaccatgccg
     1621 tgcgagtgac caacccggtc tacgcggggg cggcatccag caacgccgtc acgttcaggg
     1681 tgaacgcgcc ggagccccgg atcgaaacga ttcgcccgtt ggccgtggcc gccggtatca
     1741 gcgcgtcccg cgcgatcgat gtcgtgggcg tggatgacgc ggccaattac gtcggctttc
     1801 tcgcgggcgc gagcctccgg gtcgaagatt cggtcaaccc atcacagagc ctcaactgta
     1861 ccgcgcaagc cgtgagcggg gtcaccgtgc cgaccacggc gaacccgtgc atctacgtcg
     1921 gccccgggtc gttgcgcttc tattgggaca cggatcggtc cgttgatccc gtcagtccgt
     1981 tgttggggcc gggatcatac agcgtgaacg tgaccaatcc ggccaatcgc ggcggtcttc
     2041 ccgcgccgac gccgggcgcc ttccgcgtgg tcgcggcgcc ggaactcatc agcgtcgaac
     2101 ccgccgcggg ggtcggggcg ggcgcgcgga gcgcggtgct gaccttggcg accgcgggct
     2161 tcccggccgg atccacggtg aagatcggca ctgcggagcc cgatccctcc gatgcgacgg
     2221 cgccgccgta cgtgcaggtc caggatccgc aagtgaccat gcgatcggcg ttccgagcct
     2281 ttgccgcggg ggacgggggc accgtggtga tcgacgtggc cgacgacccg gcgcaaacca
     2341 ccaaccgatt gctggcggtc acgataacga gccaggacgg tcggtatcgg tttacatgtc
     2401 ccgctccgag tggaaccggc gcctgcgcgt cggggctcat catcgccccg gcgccagcca
     2461 ttacggccat tacgcctgcg ttggcagtgg gcgccagcgg gcggaccgtg accattacgg
     2521 gcagtcggtt tgtcgcgggg ggcacgtcgg tgacgttgcc tgcggggatc agcggcacgt
     2581 gcagcgcgac cagcgccgcg gccattgact gtaccgcggt agcggtggcg tccgcggtga
     2641 cgccggggag tctctcggtc attgtcatca acccggacgg tgggcgggcg accgggacgc
     2701 tgaccattaa ccagccgccc gtgatttcgt cgctgacccc tgggaccggc gcggccggaa
     2761 gcacggtccg cgtgcagatc acagggtccg ggttccagac cggacttgcg ttggccagcg
     2821 aagcaggcgt gaccgtcact gtggtgagcg tcgcttccgg aaccgtggtg gcggattttg
     2881 cattggccgc gggtgccgcc gacgggtatc ggtcggtgac gctgacgaat cccgacggcg
     2941 gatcgacggc ggccgcacag gcgttctaca tcggcacgcc tcccgcggat gcggaatcga
     3001 ttctgtcgac gttcggacag cggggccaga gcgccatcca atacgcgcgc tccgttggtt
     3061 ccggatggtc ggggcggttc agcggtcccg cgttgtcggc gccgccggtc tggcaagtga
     3121 tgaaagcgag tccgctccgc gacgagcgtc tgctggccgt ggtggacgat cagcgcggcc
     3181 tgacgctgca tgtgtggaac ggacaagcgt ggggaggggc gttggcggcc accgggtcca
     3241 ccggactgac gcggccgacg cagacggtcg atgtggcgtt cgagcagcaa agcgggcgcg
     3301 gcgtggcggt ctacgcgatc aacgacacca cgttgcggta ccgcgtgtgg gatggaacga
     3361 gttggagcgc cgaagcctgg gtggaggact cggtcaacgg ccaacccacg tcgggacgcc
     3421 cgctgtgggt acggctcgag gcacgccccg ggaccaacga tctgatcctc gcctatgaag
     3481 atgtcaacga ccacgtggcc gcgttcgtat gggacgggac cacgcgcacg tggggcgatg
     3541 cgatcgtgtt gacgacgacc gcgccgggcc atgagagtgc gctggtggac gtcgcgttcg
     3601 agcggctcac cggccggggg ctggtggcgt gggcgatcgc gggtgatccg gccggtcggc
     3661 cgcgttaccg agtgtggaac gggggctggg gagccgaagg gatcgcgcag ctcggcgcag
     3721 gcgccgccgc caacacgttg accgcgctcc gcgtggtcgg cgatcacgcg tccgaccgcg
     3781 tcgccctggc cagcagcgac gggaccgcga tggtagccca gatgtgggac ggacagcagt
     3841 ggatcgcgac gggctccagt gctccggtca gcgtgacgct ggaacttgcc gtggagggac
     3901 gcggccggcc gttcgacctg gcctgggagg gcagcagcgg agacctcgtg atcgcctacg
     3961 cgccgcggag cgtgtccggc ggcatgtcac gcagctggtc atccgcaggc ggctggacgg
     4021 gtccggttgc gctgcccgcc ccgacgcccg agaccgtgat caccaccacg acggcgtcgt
     4081 cggtgggcgt gagcgacgcc agcctggtgc tggttgccac ggccgcgctt cccgacgccg
     4141 gactgattca aatcgacacc gagcttattc ggtattcgag caagacgacc aatacggcgg
     4201 gaacgctggc gacactcacc ggcgtgacca gaggattcgg cggaacggcc gccacggcgc
     4261 acgcggccgg cgccccggtt caattcgtcc ccgttcccgc gtggttcgac ctggccgccg
     4321 ctcgcggaac caacgggctt gcgctggcga cgttggacac gcaggggcgg gtcaccctcc
     4381 ggcgctggac cggcgcggcc tgggccgcgc caattgaaac ccaatcgcag gccgcggggc
     4441 tcgtgtcggt ctttgatccg gcattggctc gtaatgcctt ggtcgtgggt cgaggcgtgg
     4501 ccgtgacatt ggcgcagcat ctggcgggtc cgggacccgt gatttctccg cccggcacgg
     4561 tggccgacac tacggcgccc tccacgccca cgttcggggg gggcactccg gcggccacca
     4621 ctgtctcgct ctcctggacc gcggtcgggg acgacgggat g
//
LOCUS       DBNGTT010000416         7185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_867483, whole genome shotgun sequence.
ACCESSION   DBNGTT010000416 DBNGTT010000000
VERSION     DBNGTT010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_867483"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1596
                     /locus_tag="AC16BC_09350"
     CDS             121..1596
                     /locus_tag="AC16BC_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3241865.1"
                     /translation="MKRVASALIIGVVFAASSWAAGASVSAPADPRTMTFPTEPFTPP
                     KAERVTFPNGMIVYLLEDHELPLVSLSALIRTGSIYDPPDKVGLAELTGTVIRTGGTA
                     TRAGDDLDEALEFVGAEMGSWVGLDSGGASLNVLTKDVTTGVEWFAEMLMRPAFAQEK
                     IDLARNQTIEGIRRRNDHPGSIASREFRKLLYGPNHPYGRESTEATIRAITREDLVAF
                     HRRYYHPNTAMLAVTGDFQRHDMLALLRRAFDGWNRESVTWPPVPPVTEPRNEPVNLI
                     RRDVTQTQVRMGWLGITQSDPDFFALSLLDDILGGQAFTSRLFQEVRTKAGLAYSVGS
                     VLNAGRFDRGTFFLYAQTKAESTTQAIRAMRAEIDRILTAPVSEHELAEAKQAFMNSF
                     VFSFANPAQITNRQMSLEYYGLPADFLERYRANVERVTARDLLRVAKRHLTPKRLVVF
                     AVGDERRFEQPLATFGRVRTIELADPLGAMSDPRAGAARGQ"
     gene            complement(1590..2693)
                     /gene="lptG"
                     /locus_tag="AC16BC_09355"
     CDS             complement(1590..2693)
                     /gene="lptG"
                     /locus_tag="AC16BC_09355"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR3241866.1"
                     /translation="MSDTRRAVPILFSYIARQYLRVVGLALGSLVLIYLVSEVFSKAS
                     KVSQHRPEAAALVAYLALRMPRAAYEVLPLAGLIGSVLTTSLLSRQHEVTALRACGVG
                     LSRIVTPIAAVSLALAAAAFGANWSIIPGATVDAQTVKNVRIEGRPSPSTLKRNRVWI
                     RLEHRTFLNVRMTDPGTSSLYGVRLYEVGTDFQLTREIDAPRVTVKDGLWTAFEGTER
                     RFAEDGSVRVARFTERTLDLAKLPKDFGQIEVKEEHLSYPQLLAYVEDLRQAGINPGR
                     YAVDASSRLSIPFITVVMALIGIPFGLTDTRRGGWGMAVGLSLIVALAYWMVYSLSIS
                     LGRGGLLHPWVAAWLGNVLFLAVAAALLIQKRH"
     gene            complement(2690..3799)
                     /locus_tag="AC16BC_09360"
     CDS             complement(2690..3799)
                     /locus_tag="AC16BC_09360"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR3241867.1"
                     /translation="MTIISRYLLKELVPPFTLSVAALAFLLVTQQALRLIDLLVNRGV
                     DAGILIRIFTALLPAFFVVTLPIAALMATIGAFNRLSADREIIALRATGVATRRLMGP
                     AVVFSACLCAAAYALSLAGEPWSGRSFRGLTGDLLKQQATLAVTEGTFNEDIAGMTIY
                     VERVPAPNRLEGVLIVDERAAGSEWLILADHGTFLNAGDGSSFSLRLQHGRIHRALAG
                     EPDDRYQVATFQSYELKLDPSRFQASLAADEPVRSVGALRRQAQDERRETGAVSADTE
                     RALFTAYKDLAFPLATFWLGLLGVPLGLSTGPTGRMSGFAFGVLAIGLYYLVMVGADA
                     VAARGWLPALAAASLPSLLLASAAAVLVWRMDRLT"
     gene            3965..6202
                     /locus_tag="AC16BC_09365"
     CDS             3965..6202
                     /locus_tag="AC16BC_09365"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241868.1"
                     /translation="MASDKNSIILTAQRLTAKGEIDKAIEAWRSLIAETPNDGNIYNT
                     IGDLYLKKNAAKPAIEAFLKAGTTFQDAGFALKTIAVYKKIIKLDPSRMDVVLKLADV
                     NAERGIVGNAIEDYLKVARHQLKEGRGKAAIEVYRKMAALDPGNPTIGIKLAELCQKE
                     GMIREATEELTRVAGHLEATGKTEGLTEIRAKLAELGGGQPRPSAKVQQPEQSHASLP
                     SKSAETTLMTEAAPPRAEASVPAPAAPAPEPTIEPTRLKTAQEFDENTHERLRDALTE
                     TDVYVKYGMLDKAIAHVKALAQREPNDVVVQLKLKELYVSQGDNGAAADVCRRIAELY
                     SVLGRDADRDAMLREAMELNPDLAPADDPHPETSMRADVAAENPPVETATVGEAPNGA
                     ADLKGNEAVAQDSLEQDLAEADFYFQQGFDDEAKRLFEAIVRRVPQHALATERLAELR
                     ARAATAQPAAAQPETSLESSLAGAELAAAAAEPQHPSLEETSLGASLTLNASPVEEAE
                     ARASSLPPRSAETTKGEEYIDLVEALREDMERSLPPPEEEELSQELSAERELENVFRK
                     FQKGIQAQYGKEDYETHYNLGVAYREMGLINEAIGEFRLVLQSPERAISATAMIASCY
                     RAKGSLPRAEEILLDVLNNPQYAKAEGLAGLAYELGEILEIQGRHDDALAQYYRVTAL
                     EPGHKEAAGRIAQLERPRAATASHPASPATTDGSSRKGGDRTAAQKPAKQEPPRTDRK
                     KVSYL"
     gene            6227..7060
                     /locus_tag="AC16BC_09370"
     CDS             6227..7060
                     /locus_tag="AC16BC_09370"
                     /inference="COORDINATES: protein motif:HMM:NF017437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3241869.1"
                     /translation="MNYLEFYHLREQPFSTSVDPKFYYNSQQHDDALVRLMYAASERK
                     GLAVLVGEVGAGKTTVARRLLDQLDETQFESVLMIIVHAAITSQWLLRKIAAQLGVEQ
                     PAEDSPGLLAQLSQRLVELFEQDKKVVVLIDEAQMLNSRELMEEFRGILNMEIDGHKL
                     ITFILFGLPELDQTLALDGPLAQRVAIRYQLRAFTDAITRDYITFRLKVAGSEQSPFT
                     AGAISAIHRVTGGTPRLINTLCDNALLEGCLRKRTPIDETLIQEVAVTLKLSVPLASA
                     A"
BASE COUNT         1369 a         2289 c         2450 g         1077 t
ORIGIN      
        1 cattcgggat cgcgtggccg cggtgacgcc gcaggatatc cagcgggtgg cgtcgacgtg
       61 gttcgtgaag tccaatcgga ccgtcgcccg actggtccgc cccgcgtcca aggacgcgcc
      121 gtgaaacgag tcgcgtcggc cctgatcatc ggagtcgtgt tcgccgcctc gtcgtgggcg
      181 gcgggagcgt cggtttcggc gccggcggat ccgcggacca tgacctttcc cacggaaccg
      241 ttcacgccgc ccaaggccga gcgcgtgacg ttccccaacg ggatgatcgt gtacttgttg
      301 gaggaccacg agctgcccct ggtcagtctc agcgccctga tcaggaccgg gagcatctat
      361 gatccgccgg acaaggtggg gctcgcggag ttgaccggca ccgtgatccg cacgggcgga
      421 accgcgacgc gggccggtga cgacctggac gaggccctgg agttcgtcgg cgcggagatg
      481 gggtcgtggg tggggctgga ctccggcggc gcgtcgctga acgtgctcac gaaagacgtg
      541 acgaccggcg tggagtggtt cgccgagatg ttgatgcgcc ccgcgttcgc ccaggagaag
      601 atcgacctgg ctcggaacca aacgatcgag ggcatccggc gcagaaacga ccatccgggc
      661 tcgatcgcgt cgcgggaatt caggaagctc ctgtacggcc cgaaccatcc gtacgggcgc
      721 gagagcaccg aggccacgat ccgcgcgatc acgcgcgagg atctggtcgc gtttcaccgg
      781 cggtactacc accccaatac cgcgatgctg gccgtgaccg gcgattttca gcgccacgac
      841 atgctggcgc tcctccgtcg ggcattcgac ggctggaacc gggagtcggt gacgtggccg
      901 ccggtaccgc cggtgaccga accgcgcaac gagccggtca atctcatccg gcgcgacgtg
      961 acgcaaaccc aggtccggat gggatggctc gggatcacgc agtcggaccc ggacttcttc
     1021 gcgttgtccc tgctggacga catcctgggc gggcaggcgt ttacgagccg cctgttccag
     1081 gaggtgcgga cgaaagcggg cctggcctac tcggtgggga gcgtgctcaa cgcgggacga
     1141 ttcgatcggg gaacgttctt cctgtacgcg caaaccaagg ccgagtccac gacccaggcg
     1201 atccgggcga tgcgggcgga gatcgaccgg atcctgaccg cgccggtgtc ggagcacgaa
     1261 ctggccgagg ccaaacaggc gttcatgaac tcgttcgtgt tttcgttcgc caatccggcc
     1321 cagatcacca accgacagat gtcgctcgag tactacgggt tgccagcgga ttttctggaa
     1381 cgctaccggg ccaacgtcga acgggtgacc gcgcgcgatc tgctgcgggt cgccaaacga
     1441 cacctgacgc cgaagcgcct cgtggtgttc gcggtgggcg acgagcgacg cttcgaacag
     1501 ccgctggcga ccttcgggcg ggttcgcacc atcgaactcg cggatccgtt aggagccatg
     1561 tcggacccgc gggcgggggc cgcccgcggt cagtgacgtt tctggatgag gagcgccgcg
     1621 gctacggcga ggaagagcac gttgccgagc cacgcggcca cccagggatg gagcagaccg
     1681 cctcgtccca gtgagatcga gagcgagtag accatccagt aggcgagggc cacgatcagc
     1741 gacaggccca ccgccatgcc ccagccgccc cgacgcgtgt cggtcagccc gaacggaatc
     1801 ccgatgagcg ccatcacgac cgtgatgaac gggatcgaca gacgggacga cgcgtccacc
     1861 gcataccgac ccgggttgat gccggcctga cgcaggtcct ccacgtaggc caggagctgc
     1921 ggataactga ggtgctcttc cttgacctcg atctgcccga agtccttcgg cagcttggcg
     1981 agatcgaggg tgcgttccgt gaatcgggcg acgcgcaccg agccgtcttc cgcaaaccgc
     2041 cgttcggtcc cctcgaacgc ggtccacagg ccgtccttca ccgtgacgcg gggcgcgtcg
     2101 atttcacgcg tgagttgaaa gtccgtcccg acctcgtaca gccgcacgcc gtagagcgag
     2161 ctcgtgccgg ggtccgtcat ccggacgttg agaaaggttc gatgctcgag ccgaatccac
     2221 acgcggtttc gtttgagcgt ggacgggctg ggccggccct cgatccggac gtttttcacg
     2281 gtctgggcgt cgaccgtggc gcccggaatg atcgaccagt ttgcgccgaa cgcggccgcg
     2341 gccaacgcga gggagacggc cgcgatcggc gtcacgatgc ggctcaatcc gactccgcag
     2401 gcgcgcaggg ccgtgacttc gtgctgacgc gacaacaggc tggtggtgag cacgctcccg
     2461 atcagacccg ccaacggcag gacttcgtac gcggcgcgag gcatgcggag ggccaggtac
     2521 gccacgagcg ccgcggcctc cggtcgatgt tgggaaacct tgctcgcctt gctgaagacc
     2581 tcgctgacca ggtagatgag caccagggag ccgagcgcca accccaccac gcgcaggtac
     2641 tggcgcgcga tgtaggagaa caggatcggc acggctcggc gcgtgtcgct cacgtcagtc
     2701 ggtccattcg ccacaccagc acggcggcgg cggacgccag gagcaggctg gggagcgagg
     2761 cggccgccag ggcgggaagc cagccgcgag cggcgaccgc gtccgcgccc accatcacca
     2821 gatagtacaa tccgatcgcg agcacgccga aggcgaaccc gctcattcgg ccggtcggcc
     2881 ccgtggagag ccccagggga acgccgagca ggcccagcca gaacgtggcc aagggaaacg
     2941 ccaggtcctt gtacgccgtg aacaatgcgc gttccgtgtc cgcggagacg gcgccggttt
     3001 cgcggcgttc gtcttgagcc tggcggcgca atgcgcccac ggatctcacg ggctcgtccg
     3061 cggccaggga cgcctgaaag cgcgacggat ccagtttgag ttcgtaactc tggaacgtcg
     3121 cgacctggta ccgatcgtcc ggctccccgg cgagcgcgcg atggatgcgg ccgtgttgga
     3181 gccggaggct gaacgaggaa ccgtcgcccg cgttcagaaa cgtgccgtgg tcggccagaa
     3241 tcagccactc ggatccggcg gcccgctcgt cgacgatgag gacgccttcg agccggttcg
     3301 gggcgggcac gcgttcgacg taaatcgtca tgccggcgat gtcctcgttg aacgtgcctt
     3361 ccgtgacggc caaggtcgcc tgctgcttga ggagatctcc ggtgagtccg cgaaacgagc
     3421 ggccgctcca gggttcgccg gccagggaca atgcgtaggc cgcggcgcac agacacgccg
     3481 aaaacacgac ggccggtccc atcagccgac gggtcgcgac cccggtcgcc cgcaacgcga
     3541 tgatctcgcg gtcggccgag agccggttga aggcgccgat cgtcgccatc agggccgcga
     3601 tcggcaaggt gaccacgaaa aacgccggca gcagggcggt aaagatgcga atcaggatcc
     3661 cggcgtcgac cccgcggttc accagcagat cgatcaggcg caaggcctgt tgggtcacga
     3721 gcaggaacgc gagcgccgcg acgctcagcg tgaaaggggg gacgagttcc ttgagcaggt
     3781 accgcgaaat aatggtcacg gattctcccg ttgatgggcg gccccactat atcgaaaggc
     3841 cgtcggcggc gcaacgagcg ggccggcggg cgaaaaaccg gccgaaatcc ttgacacgga
     3901 ctcgacctct gttacagttt ggagcactca acccggccgc tagcggcaat ccttcggagg
     3961 atccgtggca tctgacaaga attccattat tctcaccgcg cagcgtttga cggccaaagg
     4021 ggagatcgac aaggccatcg aggcgtggcg gtcgttgatc gcggagaccc ccaacgacgg
     4081 caacatctat aacacgatcg gcgatttgta cctgaagaag aacgcggcca aaccggccat
     4141 cgaagcgttc ctcaaggccg ggaccacgtt ccaagacgcg ggtttcgcgc tcaaaaccat
     4201 cgcggtctac aagaagatca tcaagctcga tccctcccgc atggacgtcg tgctgaagtt
     4261 ggccgacgtc aacgcggagc ggggcatcgt cggcaacgcc atcgaggact atctcaaggt
     4321 cgcgcggcat caactcaagg agggcagggg gaaggccgcc atcgaggtgt accgtaagat
     4381 ggcggccttg gatcccggga acccgaccat cggcatcaaa ctcgccgaac tgtgccaaaa
     4441 agagggcatg attcgcgagg ccacggaaga gttgacgcga gtggccggcc acctggaggc
     4501 gacgggaaaa accgaggggc tgacggagat ccgcgcgaag ctcgcggaac tgggcggggg
     4561 acagccgcgg ccgtcggcga aggtccaaca gcccgaacag agccatgcgt ctctgccgtc
     4621 gaagagcgcc gaaaccacat tgatgaccga ggcggctccg cctcgggccg aggcgtcggt
     4681 gccggcgccg gccgcgcccg cgccggaacc gacgatcgag ccgacccgcc tcaagacggc
     4741 gcaggagttc gatgagaaca cgcacgagcg actgcgggac gccctgaccg aaacggacgt
     4801 ctacgtgaag tacgggatgc tcgacaaggc gatcgcgcac gtcaaagcgc tggcgcaacg
     4861 cgagccgaac gacgtggtgg tccaactcaa actcaaagaa ctctacgtgt cgcaggggga
     4921 caacggcgcg gccgccgacg tgtgccggcg gatcgcggaa ctgtacagcg tgctcggccg
     4981 ggacgcggac cgcgacgcga tgctccggga ggcgatggaa ctcaacccgg acctcgcgcc
     5041 cgccgacgac ccgcaccccg agacgtcgat gcgggcggac gtcgcggcgg agaatcctcc
     5101 ggttgaaacc gcgacggtcg gagaggcgcc gaatggcgcc gcggatctca agggcaacga
     5161 ggccgtcgcc caggattcgc tggagcagga tctggccgag gcggacttct attttcaaca
     5221 agggttcgac gacgaagcga agcggctctt cgaggccatt gttcggcggg tcccgcagca
     5281 tgcgctcgcg accgagcggt tggcggaatt gcgcgcgcgg gccgcgaccg cgcagcccgc
     5341 cgcggcgcag ccggagacct cgctggagtc ctcgctcgcg ggcgccgaac tcgcggccgc
     5401 tgccgccgaa ccccagcatc cgtcgctgga ggaaaccagt ctgggggcga gtctgacgct
     5461 caacgcctct ccggtcgagg aggccgaggc ccgggcgtcc agcctgccgc cgcgctccgc
     5521 ggaaacgacg aagggagagg agtatatcga cctcgtggag gcgcttcggg aggacatgga
     5581 acggagcctt cctcccccag aagaggagga gttgagccag gaactgtcgg ccgaacgcga
     5641 gctggaaaac gtcttccgaa aatttcagaa ggggatacaa gcccagtacg ggaaggagga
     5701 ctacgagacg cactacaatc tcggggtcgc gtaccgtgaa atggggttga tcaatgaggc
     5761 catcggcgag ttccggctgg tgttgcagtc tcccgaacgc gccatctcgg cgaccgcgat
     5821 gatcgcctcc tgttatcggg ccaagggctc gttgccgcgg gccgaagaaa tcctgctcga
     5881 tgtcctcaat aatccccaat acgccaaggc cgaagggctc gcgggactgg cttacgaatt
     5941 gggggagatc ctggagattc aaggccgtca cgacgacgcg cttgcccagt attatcgcgt
     6001 gaccgcgctg gagccggggc ataaagaagc cgcgggccgc atcgcgcaat tggagcgccc
     6061 tcgggcggcg accgcgtctc atccggcgtc tccggcgacc acggacggca gttcgcggaa
     6121 ggggggcgat cggacggcgg cgcagaaacc ggccaaacaa gaaccgccgc gcacggaccg
     6181 gaaaaaagtt tcgtatctct gagccacaag gacgtggggg gacaccgtga actaccttga
     6241 gttctaccat cttcgcgagc aaccgttttc cacctcggtc gacccgaagt tctactacaa
     6301 cagccagcag cacgacgacg cgctggttcg cctgatgtac gccgcgtccg agcggaaggg
     6361 cctggcggtc ctggtcggag aagtcggcgc gggaaaaacc acggtggcgc gccgtctgct
     6421 cgaccagctc gatgaaacgc agttcgaatc cgtgctcatg atcatcgtgc acgcggcgat
     6481 cacgtcgcag tggttgctca ggaagatcgc ggcgcaactc ggcgtggaac agccggcgga
     6541 ggattctccg ggcctgctgg cgcagctctc gcagcggttg gtggagcttt tcgagcagga
     6601 caagaaggtc gtggtgttga tcgacgaggc gcaaatgctc aacagccgcg agttgatgga
     6661 ggagtttcgc ggcattctca acatggagat cgacggccac aagttgatca cgttcatcct
     6721 cttcggcctg ccggaactgg atcagacgct ggccctcgac gggcccttgg cgcaacgggt
     6781 cgcgattcgc taccagcttc gcgccttcac ggacgccatc acgcgcgact acatcacgtt
     6841 tcgtctgaaa gtcgcgggca gcgaacagag cccgtttacg gccggcgcga tcagcgcgat
     6901 tcatcgcgtc accggcggca cgccgcgcct catcaatacg ttgtgcgaca acgcgctgct
     6961 cgagggctgt ctgcgcaagc gcacgccgat cgacgagacg ctgatccagg aagtggccgt
     7021 gaccctcaag ctgtccgtgc cgctggcctc cgccgcgtga accggccccg gtatcggctt
     7081 gcaatttgct cctcctttcg atagggtagc cgtcgcgttc gcgtgaacgc acgcgccgcg
     7141 cggaatgttg cggccggggt gggcgattcc gaatgtcgaa acgcg
//
LOCUS       DBNGTT010000417         3358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1888596, whole genome shotgun sequence.
ACCESSION   DBNGTT010000417 DBNGTT010000000
VERSION     DBNGTT010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1888596"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="AC16BC_09375"
     CDS             <1..1934
                     /locus_tag="AC16BC_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR3241870.1"
                     /translation="EPADYQISRDEAGSTLSIDFPKTQGAETQAALPVVEEVAPVPST
                     PQEPTIVAPSVGGRITSIRIVPQKTFVDVVVGAEGPLASPNVFAIKDSRLVIDFPEAQ
                     SAVKATVVKAEANTLVRQARVGQHRDPQKVRVVLDLKKAVSYAVSPRESGVAVRLTTA
                     AGTTSKPAEAPTAKSEPAPVAPAVSAAPAPGPVAAVAPPAASQPVAAAPTAESTPAPA
                     VMVPVAKSGPKTIGDAGFSGQKISLDFQDAEVSNILRLIADVSDLNLVVGDDVKGKTT
                     LKLFNVPWDQALDIILKSKGLGQVREGNIIRIDTNTNIAKQQDEAAKAKDAQTKAEDL
                     KTAIIPINYAKATDLATTLKKNLSTRGELTVNESTNSLIAKDVERVVKDIQQLIKLLD
                     LPNPQVLIETRIVQANTNFARDLGVQWGAAYSDTPGSSQLNLNAGATGSFNSQTPTFA
                     VNLPAAGVAGPIGNVGVQWGKLSGALNLDLRLSAGEALGETKIISSPRVVTIDNKEAV
                     IQQGDSIPYETVSDKGTQTQFVDATLNLTVTPHITPNGSVIMKIKATKNAIGSFSSSK
                     TGAPSISKREASTEVMVQDGETTVIGGIFETTKGESLSGVPWLYKIPLVGWLFKREST
                     TTTNQELLIFITPTIVKRA"
     gene            1931..>3358
                     /gene="aroB"
                     /locus_tag="AC16BC_09380"
     CDS             1931..>3358
                     /gene="aroB"
                     /locus_tag="AC16BC_09380"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3241871.1"
                     /translation="MTRLRGLGENVVLLGAMGSGKSTVGRRLAERLGMAFVDTDALVE
                     REAGRSIAQIFQQDGESKFRSLERRAIAAAIAGRGRVIATGGGAILDPVTRVRLKRAG
                     VTIWLKTQPEVAAERLGGSGDRPLLASGQTTDTLTRLLARREPYYREALIHVDTSHQS
                     VEGVAAAIAARLRPASTVVPVSAGAFRYDVSIGWATLDRLGECVSGRHRACTVAVITN
                     PRVGGLYADRVTASLRAVGFRPFLVRVPDGERYKTLRSAGRLYDELVRRRFERGDLIV
                     ALGGGVIGDLAGFVAATYLRGVDYVQVPTTVVAQVDSSIGGKTGVDHRQGKNLVGAFH
                     HPVGVYADVSTLATLPRREFVAGLAEVVKYGIIADATLIEYLEEHANAVLGGVPDVLA
                     EIVRRSASVKADVVRQDEREVGLRKILNYGHTAGHVIETLTGYSAVRHGEGVAMGMDF
                     AARLALRLGLCDETLVRRQQALLERL"
BASE COUNT          676 a          936 c         1106 g          640 t
ORIGIN      
        1 ccgagcccgc cgattatcaa atttcccgcg acgaagccgg gagcacgctg tccattgatt
       61 ttccgaagac acagggggcg gagacccagg ccgcattgcc tgttgtggag gaagtggctc
      121 cggtaccgtc gacaccccaa gaaccaacga tcgtcgcgcc gagtgtcggg ggacgtatta
      181 cgtcgatccg aatcgtgccg cagaagacct tcgtggacgt tgtggttggt gcagagggtc
      241 cgctggcgtc acccaatgtg ttcgcgatca aagattcccg attggtgatc gatttcccgg
      301 aggcgcaaag cgcggtcaag gccacggtcg taaaggcgga agccaatacc ttggtgaggc
      361 aggcgcgagt cgggcaacac cgcgatccgc agaaagtccg ggtggtcttg gatctaaaga
      421 aggcggtttc ctatgcggtg tcaccgcgcg agtcgggagt cgcagtgcga ctcacgaccg
      481 ccgccggcac gacgtcgaaa cctgccgaag ctcccacggc gaagtctgag ccggcgcccg
      541 tagcgccggc cgtctcggcc gcgccagccc ctggtccggt cgcggcagtg gcaccgcctg
      601 ctgcgtccca gcccgtggca gcggctccga ccgcggaatc aactccggcg cctgcggtga
      661 tggtcccggt cgcgaagtcc ggcccgaaga cgatcggcga cgcggggttt agcggtcaga
      721 aaatctccct ggattttcag gacgcggagg tctccaacat tctgcggctc attgccgatg
      781 tcagcgatct caatttagtg gtgggcgacg acgtcaaagg gaaaacgaca ttgaagttgt
      841 tcaacgtacc gtgggatcag gcgttggaca ttatcttgaa atcgaagggg ttgggccagg
      901 tccgggaggg aaacataatc cggatcgaca ccaacaccaa catcgcaaaa caacaggacg
      961 aggccgcgaa ggcaaaggat gcccagacaa aggccgagga cctcaagacg gcgatcatcc
     1021 ccatcaatta tgcgaaggcg acggatctgg ctacgacgtt gaagaagaac ctcagtactc
     1081 gtggagaact cacggttaac gaatctacga acagcttgat tgcaaaggat gttgaacgcg
     1141 tggtcaagga tattcagcag ctcatcaagc tgcttgacct cccgaatcca caggtcttga
     1201 tcgagacgag gatcgttcag gccaacacga actttgcgcg ggaccttggc gtacaatggg
     1261 gggcggcgta ctcggatacg ccgggatcca gccaactgaa tctgaatgcg ggcgcgacag
     1321 gatcatttaa ttcgcagacg ccgacgtttg ccgtgaacct accggccgcc ggcgttgccg
     1381 gaccgatcgg caacgtgggc gtccaatggg ggaagctgtc gggggcgctc aatcttgatc
     1441 tccggttgtc ggctggtgaa gcgttgggcg agaccaagat tatttccagt ccccgtgtgg
     1501 tcacgatcga caacaaagag gctgtgatcc aacaaggtga ttccattccc tatgagacag
     1561 tatccgacaa aggaacgcag acacagtttg tcgacgcgac cttgaacctg actgtcacgc
     1621 cgcacattac gccgaacggc agcgtgatca tgaagatcaa ggcgacgaag aacgccattg
     1681 gaagtttctc cagttcgaaa accggcgctc caagtatcag caaacgcgaa gcttcgactg
     1741 aagtcatggt tcaagatggg gaaacgacgg tgatcggtgg gattttcgag acaacgaagg
     1801 gcgagtccct cagcggtgtc ccgtggcttt ataagatccc cttggtcggc tggctattta
     1861 agcgcgagag caccaccacg accaatcagg agttgttgat ttttatcacc ccgacgatcg
     1921 ttaagcgtgc gtgacccggt tgcgtggtct tggagagaac gtggtgttgc tcggagcgat
     1981 gggctccggc aaatccacgg ttggtcgtcg cctggccgag cgcctgggaa tggcttttgt
     2041 agacaccgat gctctcgttg aacgcgaagc cggtcggtcg atcgctcaaa tcttccagca
     2101 ggatggtgag tcgaaattcc ggtccttaga gcgacgggca atcgccgccg cgattgcggg
     2161 gcggggacgc gtcattgcca ctggcggcgg tgcgattctc gatccggtga cccgcgtgcg
     2221 actcaagcgc gcgggtgtca cgatttggct caagacccaa ccggaagtgg cggccgagcg
     2281 cctgggagga tccggcgatc gtccgctgct cgcgtccggg cagaccaccg atacgttgac
     2341 acgcctcctc gctcgacgcg agccgtatta ccgcgaagcg ctcatccacg tggacacgtc
     2401 ccaccagagc gtcgaagggg tggcggcggc gatcgccgca aggctccggc cagcctcgac
     2461 ggttgtcccg gtttccgcgg gagcgttccg atacgatgtg agcattggct gggcgaccct
     2521 cgatcggctc ggtgagtgtg tgagcgggcg acaccgcgcg tgcaccgtag ccgtgatcac
     2581 gaatccacgg gtcggggggc tgtatgccga tcgtgtgacg gcgagtctgc gggccgtggg
     2641 gttccgacca tttctggtcc gggttccgga cggggagcgg tacaagacgc tccgttcggc
     2701 tggtcgtctt tacgatgaac tggtcagacg gcgatttgaa cgaggcgatc ttatcgtcgc
     2761 cttgggcggc ggcgtcattg gagacctcgc gggatttgtc gcggcaacgt atttacgcgg
     2821 ggtggactat gtgcaggttc cgacgacggt ggtggcccag gttgattcga gcatcggcgg
     2881 gaagacgggc gtcgatcatc gacaggggaa gaacctcgtc ggcgccttcc atcatccggt
     2941 gggggtctac gccgatgtct cgacgctggc cacgttgccg aggcgcgaat ttgtcgcggg
     3001 gttggccgag gtcgtcaagt acggcataat cgccgacgcc acgttgatcg agtatcttga
     3061 agagcacgcg aatgccgtgc tgggcggagt gcccgacgtt ctggcggaaa tcgtccgtcg
     3121 gtcggcttcc gtgaaggccg acgtggtgcg gcaagacgaa cgcgaggtgg ggcttcgcaa
     3181 aattctcaat tacggccaca ccgcgggcca cgtcatcgag acgttgacgg gctattcggc
     3241 cgtccgtcac ggcgaagggg ttgcaatggg gatggacttt gccgcccggt tggccctcag
     3301 actcggtttg tgtgatgaga cgctcgtgcg tcgtcagcag gcgttgttgg agcggctc
//
LOCUS       DBNGTT010000418         3805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3549859, whole genome shotgun sequence.
ACCESSION   DBNGTT010000418 DBNGTT010000000
VERSION     DBNGTT010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3549859"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /gene="cas4g/cas1g"
                     /locus_tag="AC16BC_09385"
     CDS             complement(<1..1354)
                     /gene="cas4g/cas1g"
                     /locus_tag="AC16BC_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas4g/Cas1g"
                     /protein_id="HIR3241872.1"
                     /translation="MDTADDAPAAGAEMPPLPVRMLNEFTYCPRLGYLMWVQGEFADT
                     ADTVDGRFQHRRVDQDKKRRAPKPTAESDGPATIHARSVSLTADRLGLTAKVDLVEGE
                     GNRVTPVDYKRGKRPHTERGAWEPEMVQLCAQGLILRENGFECEEGVLYFVSSRERVT
                     ITFDDALVARTLDLAAQMRRAAHEGVVPPPLVDSPKCPRCALVSICLPDEVHFLGDRA
                     GGHEPRPLFPMRDDALPLHVQHQGAKVRKNGDVLKITDGDEVLGEARIREVSQLVVLG
                     GVQVSTSVVHELCKRGVPISYLSGGGWFYGVTHGMSHKNVELRRHQYQAAASRERSLS
                     LARRFVQAKIANCRTLLRRNAAGVTEDVLADLKGDIRRAGDTATMESLLGIEGNSAQR
                     YFSWFSKMITGDVTTFDFQTRNRRPPKDPINALLSLAYAMLTREWTTTLLSVGFDPYL
                     G"
     gene            complement(1429..1827)
                     /locus_tag="AC16BC_09390"
     CDS             complement(1429..1827)
                     /locus_tag="AC16BC_09390"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR3241873.1"
                     /translation="MSEREDSPYSEDWLRVARQDWHRIHVMLADGDGDAAGLFLQQAI
                     EKFLKAFLLEHGWKLKKVHTLQSLLDEAVAFKSTLAPFRPLCEKVSGFYFAERYPSLG
                     GEELDAEDVRRELDHARGLILALFPREVPE"
     gene            complement(1824..2153)
                     /locus_tag="AC16BC_09395"
     CDS             complement(1824..2153)
                     /locus_tag="AC16BC_09395"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3241874.1"
                     /translation="MRPTTEVADRVRLRAIADRLRERLGAQRVILFGSAARGEATEHS
                     DIDLLVVAPTTDRFYDRMGAALAAVRDISAGMPLAPIVLTPEEVAARLRRGDQFVQEI
                     VETGVSL"
     gene            complement(2505..>3805)
                     /gene="csb2"
                     /locus_tag="AC16BC_09400"
                     /gene_synonym="csx5"
     CDS             complement(2505..>3805)
                     /gene="csb2"
                     /locus_tag="AC16BC_09400"
                     /gene_synonym="csx5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-G CRISPR-associated protein Csb2"
                     /protein_id="HIR3241875.1"
                     /translation="VATASPVLFCWRTLTVEEKQRSLLWDILQHVSYFGRAESLCNLE
                     LVDGKVFPDDGWCRPCLNNGNPIRRIAENCRDVFCPDPQGFQANDLWSRRAGRDALDR
                     NNAPPHLVEDLLAHQPLPDGGRWVSYEMPVGWPGRWVVRVAKSARRKSIPVLPAPRIA
                     HYLRFSLQCRIPVPLKFTVPLAEQFRKRALAHYGRANANGSSFALSGHNRPDDVEGEH
                     QHAFYLPLGTDRPRPDLLNELHVWCPYGFTQAEVEALMRVDRLDWGSGKHPLRPVLMA
                     IGNTVPHECQISIGQISSRIWRSRTPFVPPRYFYRGNLHRAKLKAKDAPEQQLAQCLR
                     QAGIDTTGEIRRLTPNGQTQQSIPPLSEWDIVRAPESEEEALTSGIITAVHVPTNSAS
                     VEKTRRIGLFFEIAFDVPVSLRVPALGHSAHFGLGLFIAG"
BASE COUNT          745 a         1255 c         1079 g          726 t
ORIGIN      
        1 aacccaagta gggatcgaat cccacgctca gcaacgtggt ggtccactcg cgcgtcagca
       61 tggcataggc gagcgacagg agcgcgttga tcgggtcttt cggcggccga cggttccgag
      121 tctgaaaatc aaacgtcgtg acgtcgcccg tgatcatctt cgagaaccac gaaaaatacc
      181 gctgggccga attcccctcg attcccaaca gcgattccat ggtggccgtg tcgcccgcgc
      241 gtcggatgtc gcctttgaga tccgccagca cgtcctctgt cacgcccgcg gcgttccgcc
      301 gaagcagcgt gcggcagttg gcgatcttgg cttgcacgaa tcggcgggcg agcgaaaggc
      361 tccgctcacg gctcgccgcc gcctgatact ggtgccgccg cagttccacg ttcttatgac
      421 tcatcccgtg ggtcacgccg tagaaccacc cgcccccgga caggtacgag atcggcacgc
      481 cccgcttaca cagctcgtgg accaccgacg tgctgacttg cacccctccc agcaccacca
      541 gttgcgagac ctcgcgtatc cgcgcctcgc ccagcacctc gtccccgtcc gtgatcttca
      601 gcacgtcccc gttcttgcgc accttcgcgc cttggtgctg cacgtgcagc ggcaacgcat
      661 catcccgcat cgggaacaac ggccttggct cgtggccgcc agcccgatcc ccgagaaagt
      721 ggacttcgtc aggcaggcaa atgctcacca atgcgcaacg tgggcacttg gggctatcaa
      781 cgagcggtgg cggaaccaca ccctcgtgcg ccgctcgccg catctgcgcg gccagatcca
      841 gcgtgcgagc cacgagcgcg tcatcaaacg tgatcgtcac ccgctcacgc gagctgacga
      901 agtacagcac cccttcctca cactcgaagc cattctcacg gaggatgagc ccctgcgcgc
      961 acaactgcac catctccggc tcccatgccc cgcgctccgt gtgcggccgc ttgccgcgct
     1021 tatagtcaac cggcgtgacg cggttcccct cgccttcaac cagatcgacc ttggccgtca
     1081 accccaagcg atccgcggtc agggacaccg accgcgcatg aatcgttgcc ggaccgtcag
     1141 actcggcagt cggcttgggc gcccggcgct tcttgtcttg atccacccgc ctatgctgaa
     1201 accgcccatc caccgtgtcc gccgtatccg caaactcccc ctgcacccac atgagatagc
     1261 cgagtcgcgg acagtacgtg aactcgttga gcatccgaac cggcaggggc ggcatctccg
     1321 cccctgccgc gggagcatca tcggctgtgt ccataggaac actcctattc agttgtcaaa
     1381 gaacgaccga ccacgaacgt ggtacccaac gtgccgaccg ggcctatctc actccggcac
     1441 ctcccgcggg aacaacgcga ggatcagacc ccgcgcgtga tcaagctccc gacggacgtc
     1501 ctctgcatcg agttcctctc ccccaagact cggataacgt tcggcgaaat agaaccccga
     1561 taccttttca cagagcgggc gaaacggtgc gagtgttgac ttgaacgcca ccgcctcatc
     1621 gaggagactt tgcagcgtgt gaaccttctt gagcttccac ccgtgctcca gaagaaacgc
     1681 cttgagaaat ttctcaatcg cctgttgaag aaataagccc gcggcgtctc cgtccccgtc
     1741 tgccagcatc acgtggatcc gatgccagtc ctgtcgcgca acccgcaacc aatcctcgct
     1801 gtatggcgaa tcttcgcgct cgctcacaga ctcaccccgg tctccacgat ctcctggacg
     1861 aactgatcgc cgcggcggag ccgagcagcc acctcctccg gagtcagcac gatcggcgcg
     1921 agcggcatgc ctgcgctgat gtcgcgcacc gcggccagcg cggcccccat acggtcgtaa
     1981 aatcggtccg tggtcggcgc gaccaccaag agatcgatgt cgctgtgctc ggtcgcctca
     2041 ccacgggccg ccgacccgaa gagaatcacc cgctgggctc ccaaccgttc ccgaagacgg
     2101 tcagcgatgg cgcgaaggcg aactcggtcc gccacttcag tcgtcggtct catcacatgc
     2161 acctcctcat atccctcttt ggatccctct ttcttgcccc gtttccgaga tagtaccctt
     2221 tcccgtgatg tctcccaccg gcgcgttcga cccgtgcatg ctcgcgcagc acgcgcagcc
     2281 gctgcggcga ctgcttcgtg aacagctcct gccgccctcg cgcctcgccc agatccgcga
     2341 gataccacgc cgtggtcagc ggaacggtct caggcttggc ggcaaattgg cgaagcgtgt
     2401 tcatcttcga tcaaatccac ttctcgggca tgcgattcaa catcacgagg tcgaacccga
     2461 ctccgtggac cagaaacggt tcctgccatg ttttcgattc cggatcaacc cgctataaac
     2521 agcccgaggc cgaaatgcgc agagtgccca agagctggaa cgcgaaggct cacagggaca
     2581 tcaaaagcaa tctcgaagaa cagaccaatg cgacgagtct tttccacgct agcggagttt
     2641 gtcggaacat ggacggcagt tatgataccg ctggtcaagg cttcttcttc gctctccggg
     2701 gcgcgaacaa tgtcccactc tgacaagggt ggaatggatt gttgggtctg tccgttgggt
     2761 gtcagacggc ggatttctcc ggtcgtgtcg atccctgcct gccggaggca ttgagcgagt
     2821 tgctgttccg gtgcgtcttt ggccttcagt ttcgcgcggt gcagatttcc ccggtagaag
     2881 tagcgcggag gaacaaatgg cgtgcggctt cgccagatgc gactcgaaat ctgcccaatg
     2941 gagatctggc attcgtgcgg aaccgtgttg cctattgcca tcagaaccgg cctgaggggg
     3001 tgttttccgc ttccccaatc gagacgatca acgcgcatca gggcttcgac ttcggcttgg
     3061 gtaaagccgt agggacacca gacgtgaagc tcgttcagta aatcgggacg aggccggtcc
     3121 gttcccagtg gaagatagaa ggcatgctgg tgttcaccct caacatcgtc tggtctgttg
     3181 tggcctgaca acgcgaaaga gctgccgtta gcgttggcgc gcccataatg cgcgagggct
     3241 ctcttcctga actgttccgc gagaggcacg gtgaacttga gtggcacagg aatgcggcat
     3301 tgaagagaga accgcagata gtgtgcaatg cggggcgccg gtaatacagg tatcgatttg
     3361 cgtcgtgcgg acttggcgac acgcacgacc catcgtcccg gccagccaac cggcatttcg
     3421 taggacaccc accgtccgcc gtcgggcagg ggttggtgtg ccaacaggtc ttcgacaaga
     3481 tggggcggag cgttgtttcg atcgagtgca tcgcgaccgg cgcggcgaga ccagagatcg
     3541 ttcgcttgaa agccctgtgg atcggggcag aacacgtcgc ggcagttttc ggcgatgcgg
     3601 cgaattggat ttccgttatt caggcaaggc cgacaccagc cgtcatcggg aaagaccttg
     3661 ccatccacca gttccagatt gcacagtgat tccgcacggc caaaatagct gacgtgctgg
     3721 aggatgtccc aaagcaatga gcgttgtttc tcctcaactg tgagggtcct ccagcagaat
     3781 aagaccgggc ttgcggtcgc gacaa
//
LOCUS       DBNGTT010000419         2845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2272317, whole genome shotgun sequence.
ACCESSION   DBNGTT010000419 DBNGTT010000000
VERSION     DBNGTT010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2272317"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="AC16BC_09405"
     CDS             complement(<1..491)
                     /locus_tag="AC16BC_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR3241876.1"
                     /translation="MKVLVIGSGGREHALVWKLAQSPRVAKIYCAPGNGGIARSATCL
                     PIAVDDAEGLLAAAGREGIDLTVVGPEAALAAGVVDRFEAAGRRIVGPARWAAQLETS
                     KAFSKAFMQRHGIPTAKAVVARSAQEALAVLDLHPLPVVIKADGLAAGKGVVVTADRD
                     EAR"
     gene            complement(842..2395)
                     /gene="purH"
                     /locus_tag="AC16BC_09410"
     CDS             complement(842..2395)
                     /gene="purH"
                     /locus_tag="AC16BC_09410"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR3241877.1"
                     /translation="MRRALISVSNKEGVVDVAKGLVGLGFELVSTGGTAKLLREQGLA
                     ARDISEITGFPEMLDGRVKTLHPNVHGGLLGRRRLPDHVEQMKRHGIEPIDVVIVNLY
                     PFEATISKPGCAFEDAIENIDIGGPSMLRSAAKNFEEVAVVVDPADYPLLLDGLKRPE
                     GLSRETRLAFARKVFAHTAKYDGVIAAYLDRQAPAAAPETRFPQTLILRYEKLQNLRY
                     GENPHQQAAIYRDPTVIEACTARARQLHGKEMSYNNFLDAHAALELVKEFETCAVVIV
                     KHNNPCGVALGPTPADAYRKAREGDPVSAFGGVAAFNRPVDGETAKEIGSTFMEVIVA
                     PGFTPEAVEELGRKKALRLLDIGPLGAGAPEAMEVRKIVGGLLYQERDLGRIADVRAL
                     RVVSKRTPTAEEYDALAFAWKVAKHTKSNAIVYARADRTVGVGAGQMSRVDSVKIGAM
                     KAVLPLSGTVIASDAFFPFRDGIDEAAKSGVTAVIQPGGSVKDEEVVGAANEHGMAMV
                     LTGMRHFRH"
     gene            complement(2440..>2845)
                     /locus_tag="AC16BC_09415"
     CDS             complement(2440..>2845)
                     /locus_tag="AC16BC_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241878.1"
                     /translation="KGAGNAKPGEVGEEFFRRLSATIDAVHARGVVLCDLRNMNNVLV
                     SDRGDPYVIDFAAAFRRGAWWNVPVNALFRLFVQDDCMGVIKLKRRLAPSLLTHEESE
                     RYDKGVFLQGPAIAVRNWGRRWLKRLVGMKTS"
BASE COUNT          455 a         1039 c          885 g          466 t
ORIGIN      
        1 tcgcgcgcct cgtcgcggtc ggccgtgacc accacgccct tgcccgcggc caggccgtcg
       61 gccttgatga ccaccggcaa cggatgcagg tcgagcacgg cgagcgcttc ctgcgccgaa
      121 cgcgcgacca cggccttggc ggtcggaatg ccgtggcgct gcatgaacgc tttggaaaag
      181 gccttgctgg tttccaactg cgcggcccaa cgcgcgggcc ccacgatccg tcgcccggcg
      241 gcttcgaacc ggtccaccac gcccgccgcg agcgcggcct cggggccgac cacggtcagg
      301 tcgatgcctt cgcgtccggc cgcggccagc aacccctccg cgtcgtccac cgcgatcggc
      361 aggcaggtcg cggaccgggc gatccccccg ttgcccggcg cgcaatagat cttcgcgacc
      421 cgcgggctct gcgccagctt ccacaccagc gcgtgttccc gcccgccgct cccgatcacc
      481 agcaccttca tcgccggccc tcgaaccgca gcggccctcc ctggccgaag aaggaaaaac
      541 cgaatgggag agggggggaa ggggagaatc ggggacacga atcaggtcgt tccgccctga
      601 ctccgattcg ccgatgagca aatcccccca ccctcccctc ccccgcaagg ggggagggaa
      661 aaaaggagtc agtgaagttg aggttttccg caggcagccc gaaaggctgc cctgcgagcc
      721 cgttcaggca tggacagatg caaggcggag ggagactttg cgcgcggagc gtactgggtc
      781 gtacgtgagc acgcaaagcc gaccgacaac gccgcagatg gccgtgcatg gacgggctca
      841 cctagtggcg gaagtggcgc atgcccgtga ggaccatggc catcccatgc tcgttcgccg
      901 cccccaccac ctcctcgtcc ttcaccgatc cgcccggctg aatcaccgcg gtcacgcccg
      961 acttggccgc ctcgtcgatg ccgtcccgaa acgggaaaaa cgcgtccgac gcgatcacgg
     1021 tgccggacag cggcagcacc gccttcatcg cgccgatctt gaccgaatcc acgcggctca
     1081 tctggcccgc gcccacgccc acggtccggt ccgcccgcgc gtacacgatg gcgttggact
     1141 tggtgtgctt ggccactttc caggcgaacg ccaaggcgtc gtattcctcg gcggtcggcg
     1201 tgcgtttgga caccacgcgc aacgcccgca cgtccgcgat ccgccccagg tcccgttcct
     1261 ggtagagcag gccgcccacg atctttcgca cctccatcgc ctcgggcgcg ccggccccca
     1321 acggaccgat gtcgagcaga cgcagggctt tcttgcggcc gagttcctcg accgcctcgg
     1381 gcgtgaaccc gggcgcgacg atcacctcca taaacgtgga cccgatctcc ttcgcggttt
     1441 cgccgtccac cggacggttg aacgcggcca cgcccccgaa cgcggacacc gggtcgccct
     1501 cgcgcgcctt gcggtacgcg tcggccgggg tcgggcccaa cgccacgccg caggggttgt
     1561 tgtgcttcac gatcaccacc gcgcaggtct cgaactcttt gaccagttcg agcgcggcgt
     1621 gcgcgtccag gaaattgttg tacgacattt ctttgccgtg cagttgacgg gcgcgcgcgg
     1681 tgcacgcctc gatgaccgtg gggtcgcggt agatcgcggc ctgctggtgc gggttttccc
     1741 cgtagcgcag gttctggagt ttttcgtacc gcaggatcaa ggtctgcggg aaccgcgtct
     1801 cgggcgcggc ggccggcgcc tggcgatcca ggtacgcggc gatcacgccg tcgtacttgg
     1861 cggtgtgggc gaacactttg cgcgcgaacg cgaggcgcgt ctcgcggctc aggccttcgg
     1921 gccgcttgag tccgtcgagc agcagcgggt agtccgcggg gtccaccacc accgccacct
     1981 cttcgaaatt cttcgcggcc gaacgcagca tcgagggccc gccgatgtcg atgttctcga
     2041 tcgcgtcctc gaacgcgcag ccgggcttgc tgatggtggc ttcgaacgga tagagattga
     2101 cgatcaccac gtcgatgggc tcgatgccgt ggcgcttcat ttgttcgacg tgatcgggca
     2161 atcgccgccg tccgagcaac ccgccgtgga cgttcgggtg caaggtcttc acccggccgt
     2221 cgagcatctc cgggaagccc gtgatttcgg aaatgtcccg cgccgccaac ccttgctcgc
     2281 gcaacagctt ggcggtgccg ccggtggaga ccagttcaaa gcccaggccg accaggccct
     2341 tggccacgtc aaccacgccc tctttgttcg acacgctgat caatgcccgc cgcatgatgt
     2401 cctccgaatc ccctcaccgc cgaccgtctc accggacggt cagcttgttt tcatccccac
     2461 caatcgcttg agccaccggc ggccccagtt gcgcaccgcg atcgccggcc cctgcaggaa
     2521 cacgcccttg tcataccgct cggactcttc gtgcgtcagc aaggacgggg cgagccgccg
     2581 cttcaacttg atcacgccca tgcaatcgtc ctgaacgaac aaccgaaaca acgcgttgac
     2641 cggcacgttc caccacgcgc cccgccggaa cgccgcggcg aagtcgatca cgtacggatc
     2701 gccgcggtcg ctgaccagca cgttgttcat gttgcgcagg tcgcacagca ccacgccgcg
     2761 cgcgtggacc gcgtcgatcg tggcgctcaa ccgccggaag aactcttctc cgacctcacc
     2821 gggcttggcg ttccccgcgc ccttg
//
LOCUS       DBNGTT010000420         2701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1250378, whole genome shotgun sequence.
ACCESSION   DBNGTT010000420 DBNGTT010000000
VERSION     DBNGTT010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1250378"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /gene="lpxD"
                     /locus_tag="AC16BC_09420"
     CDS             complement(<1..1087)
                     /gene="lpxD"
                     /locus_tag="AC16BC_09420"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR3241879.1"
                     /translation="MTDASRRPASSATAPVRTTTLAALAAHVGGVLKGEDCPITGVAG
                     IREAEPGQVTFLSNPRYAADAARTRASGVVIAARVPSEGVARIEVDDPYAAFCRIVRW
                     FHDRPYRGGGISPHAVIAPDAVIGADPTIAPFVTIGRRTRLGDRVTLLAGAAVGDDVV
                     IGDDTLVHPNVTIRDGCAIGSRVILQSGTVIGGDGYGFATRGGRHEKIPQVGRVVIED
                     DVELGANVCVDRAALGATVIKRGTKVDNLVHIAHNVVVGEDCLLVAQVGISGSTELGR
                     NVVLAGQVGVTGHLKIGDRVMVGAQGGVIADIEPGQVVSGTYAMPHPTWLRVQAVLPT
                     LPEMRRTIKHLEARVAELEQRASRPAPA"
     gene            complement(1133..1699)
                     /locus_tag="AC16BC_09425"
     CDS             complement(1133..1699)
                     /locus_tag="AC16BC_09425"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3241880.1"
                     /translation="MAGRRRSALSAWIGWIALAAWSLGGSAWVQAAEGFKVAYLDTQK
                     VFDQSKAGKKAKGMLEEYVKSRQKIIDLDEAEIKELEDGLVKQESVLSPEAKRTKQEE
                     LQRKLLAYQKKAGDLNREIQDKKIEVLNDFHGQLQGVVKRVAEREGYAMVFDKAGGGP
                     ADVAAVLYAKESLSITDKVLAELDKEAK"
     gene            complement(1719..>2701)
                     /locus_tag="AC16BC_09430"
     CDS             complement(1719..>2701)
                     /locus_tag="AC16BC_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR3241881.1"
                     /translation="SFREPYLFDRPVAGTIDLYSNERRLTTYRDGRTGFSLSLSRWFG
                     EYVSAGVSYTREATRLFDLTTRVVYDPTPPNPVTGVDPVIGDEVRAYEAPPAIVQEAL
                     RGKVTTSSVGVSVARDTRDFAFDPREGQRHSLSIEYAGGLLQGDNRFYKIVSDHSAFF
                     PLFWNTVFSLHLRFGYLGGQQYPFSERFVVGGINTVRGFDYGKAGRVDPSTGEIFGGN
                     KELIFNAEYLFPLVQEAKMKGVLFFDAGRAFDDQGRYDAGGAVLIAPDGRPMSSGERL
                     RFGDLRYSVGFGIRMLLPIGPIRLEWGYNLDAQPGEKSKPFPEFTIGSVF"
BASE COUNT          432 a          988 c          822 g          459 t
ORIGIN      
        1 ccgcgggcgc cggccggctc gcgcgctgtt cgagttccgc gacgcgcgcc tccaggtgct
       61 tgatcgtccg ccgcatctcc ggcagcgtgg gcaacacggc ttggacccga agccacgtgg
      121 ggtgcggcat cgcgtacgtg cccgacacga cctgccccgg ctcgatgtcc gcgatcaccc
      181 cgccctgggc gcccaccatg acgcggtccc cgatcttgag gtgaccggtc acgccgacct
      241 gtccggccag caccacgttg cgcccgagtt ccgtgctgcc cgaaatcccg acctgggcca
      301 ccagcaggca gtcttcgccg accaccacat tatgggcgat gtgcaccaga ttatcgacct
      361 tggtgccccg tttgatgacg gtcgcgccca gcgcggcccg gtccacacac acgttcgcgc
      421 ccagttccac gtcgtcctcg atcaccacgc ggccgacctg cggaatcttc tcgtggcgcc
      481 ccccgcgcgt ggcgaagccg tatccatcgc ccccgatcac cgtcccgctc tgcaggatca
      541 cgcgcgagcc gatcgcgcag ccgtcgcgga tcgtgacgtt ggggtgcacc agcgtgtcgt
      601 ccccgatgac gacgtcgtcg cccaccgcgg cgcccgcgag cagcgtgacc cgatccccga
      661 gtcgcgtccg gcggccgatg gtgacgaagg gggcgatcgt ggggtcggcc ccgatcacgg
      721 cgtcgggcgc gatgaccgcg tgcggactga tcccgccgcc gcgatagggc cggtcgtgga
      781 accaccgcac gatgcggcag aacgcggcgt aggggtcgtc gacctcgatc cgcgcgacgc
      841 cctcgctcgg aacgcgcgcc gcaatgacca cgcccgaggc ccgcgtccgc gccgcatccg
      901 ccgcgtaccg cgggttggac aggaaggtca cctgacccgg ttcggcttcg cggattccgg
      961 ccacgccggt aatcgggcaa tcctcgcctt tgagcacgcc gccgacgtgc gcggcgagcg
     1021 cggcgagcgt ggtggtccgc accggggccg tggcggagga cgcgggacgg cgcgacgcgt
     1081 cagtcatgga ccgccatgat ccccgccatc ccgaccggtc gccagcgcgc cgtcacttgg
     1141 cctctttgtc caactccgcc aacaccttgt cggtgatcga aagcgactcc ttggcgtaga
     1201 gcaccgccgc gacgtccgcg ggaccgcctc ccgccttgtc gaagaccatc gcgtagccct
     1261 cgcgctcggc cacccgcttg accacgccct gcagctgccc gtgaaagtcg ttgagcacct
     1321 cgatcttttt gtcctggatt tcgcggttca gatcgccggc tttcttttga tacgcgagca
     1381 gcttgcgctg caactcctcc tgcttggtcc gcttggcctc cgggctcaac acgctctcct
     1441 gcttgaccag accgtcttcg agctccttga tttccgcttc gtccagatca atgatcttct
     1501 gccggctctt gacgtactcc tccagcatgc ctttggcctt tttgccggcc ttggattgat
     1561 caaagacctt ctgcgtgtcg agataggcca ccttgaatcc ctcggcggcc tgcacccacg
     1621 ccgagcctcc gaggctccac gccgccaggg cgatccaccc gatccacgcg ctcagggcac
     1681 tcctgcgcct tcctgccatc acgagtctcc tcctctgctc agaacaccga cccgatcgta
     1741 aactcgggaa acggcttgct cttctcgccc ggttgggcgt cgagattata cccccattcc
     1801 agtcgaatcg gcccgatcgg gagcaacatc cggatgccga accccacgct gtaccgcaga
     1861 tcgccgaacc gcaaccgctc gccggaggac atgggccgcc cgtccggcgc gatcaggacc
     1921 gcgccgccgg cgtcataccg cccctgatcg tcgaaggcgc gtcccgcgtc gaagaacaag
     1981 actcccttca tcttcgcttc ctgaaccagc gggaagaggt attccgcgtt gaagatcagt
     2041 tccttgttcc cgccgaagat ttcccccgtg ctcgggtcga cgcggccggc cttgccgtag
     2101 tcgaagccgc gcaccgtgtt gatgcccccc accacaaacc gttccgaaaa cgggtactgc
     2161 tgcccgccca agtatccgaa ccgcagatgc aacgagaaca cggtgttcca gaacaggggg
     2221 aaaaacgcgc tgtggtcgga gacgatctta tagaagcggt tgtcgccctg cagaaggccg
     2281 ccggcgtatt cgatggacaa cgagtggcgt tggccttcgc gcggatcgaa cgcgaaatcg
     2341 cgcgtatcgc gcgccacgga cacccccacg ctgctggtcg tcaccttgcc gcgcaacgcc
     2401 tcctgcacga tcgcgggcgg ggcctcgtac gcgcgaacct cgtcgccgat gaccggatca
     2461 acgcccgtga ccgggttcgg gggggtgggg tcgtacacca cgcgcgtggt caggtcgaac
     2521 aaccgggtgg cttcgcgcgt atacgacacg ccagccgaca cgtactcgcc gaaccagcgg
     2581 ctcaggctca gggagaatcc ggtgcggccg tcgcggtacg tggtcaatcg ccgctcgttg
     2641 gagtacagat caatggtccc ggccactggc cggtcaaaca ggtagggctc gcgaaacgac
     2701 a
//
LOCUS       DBNGTT010000421         5648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1378442, whole genome shotgun sequence.
ACCESSION   DBNGTT010000421 DBNGTT010000000
VERSION     DBNGTT010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1378442"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1325)
                     /gene="hypD"
                     /locus_tag="AC16BC_09435"
     CDS             complement(201..1325)
                     /gene="hypD"
                     /locus_tag="AC16BC_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR3241882.1"
                     /translation="MRFLDEYRDANAARDVAGAIRRITTRPWSVMEVCGGQTHAIVRF
                     GLDELLPEQVTLLHGPGCPVCVTPVALIDAAIEIGSRHGAIVCSFGDMLRVPGSGRDL
                     LSVKAEGGDVRIVYSPLDALALARRFPDRQVVFFAVGFETTAPANALAVYQAERDGVE
                     NFSVLVAHVLVPPAMASILESPGNRVDGFLAAGHVCAVMGYTQYEPLAARFRVPIVVT
                     GFEPLDILQGIYQCVKQLEEGRAEFENQYARAVRRTGNSGAQEIVRRVFEASSRTWRG
                     IGDIAESGLVLRPRYARFDALRRFPLASRSAEEAGECVAGEIMLGAKIPADCAAFGTR
                     CTPDHPLGAPMVSSEGACAAYYRYRRPVPMFGVGARSGDV"
     gene            complement(1464..1706)
                     /locus_tag="AC16BC_09440"
     CDS             complement(1464..1706)
                     /locus_tag="AC16BC_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR3241883.1"
                     /translation="MCLAIPGRIVAVNGAEGVDRAGRVQFGGIVKDVSLAFVPEAAVG
                     DYVIVHVGFAIARVDEAEAARALEYIAELGEFGERD"
     gene            complement(1714..4188)
                     /gene="hypF"
                     /locus_tag="AC16BC_09445"
     CDS             complement(1714..4188)
                     /gene="hypF"
                     /locus_tag="AC16BC_09445"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIR3241884.1"
                     /translation="MTPLTVEARSAVTPPRVEPRRFDVLVSGRVQGVGFRPFVYRLAV
                     ELGLSGWVKNERGGVRVAAEGEPAALDRFVHRLRSEAPPHAAIDDVRATSGELIGVAG
                     FAIHPSDSEGAAGVHVPPDVMPCPECLRDVLDPSNRRYRYPFTHCASCGPRYTIIRAM
                     PYDRSNTTMARFPMCAACRAEYDDPSDRRFHAEAIACPACGPQLAAWDNDGRVESTGY
                     DAVAAAAAAIRNGRIVAVKGVGGFHLLAEATNDSAVRELRRRKHRPDKPFAVLCESLD
                     AVEAWCDVAEPERLLLQSVEGPIVLVRARSSAVAPSVAPGNPNLGVMLPPSPLHLLVA
                     RAVKAPVVATSGNRSDEPICIEERDAVERFRGIADLLLVHDRAIERRVDDSVVRVIGD
                     AAVVLRVGRGYAPLVIPVARSEVSTLAVGGGQKNTVALRTSEAVALSQHIGNLSAADT
                     RAVHRRTASDLAGLHGVTPAVTRCDRHPDDAAARDSASFGQPVVGIQHHHAHVLACRA
                     EHGLDGPVLGVAWDGTGYGLDGTIWGGEFLIVDDGSWRRVGHLRTFRLPGGEQAIREP
                     RRAALGVLVELFGERVATRDDLPPLASWSASERAVLARMASRAVNAPLTSSVGRLFDA
                     VSSLIGIRQTATFEGQAAMDLEFAAEKGHIPPMSRPEQNPPVPPFEKGGMGGFDSWGE
                     RGDLGSTGEPYPISVLEHDNILVVDWEPTVRAILDDVGRGVVVSVMARRFHEALVEAI
                     VTVAERVGEPRIALTGGCFQNRYLTERATARLTASGLTVYRHRRVPPNDGGLALGQAV
                     AYAGTNPIPPIPPLEKGGAGGFRSLP"
     gene            complement(4185..4448)
                     /locus_tag="AC16BC_09450"
     CDS             complement(4185..4448)
                     /locus_tag="AC16BC_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013334.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HIR3241885.1"
                     /translation="MHDAAWVDDLLRTVAAVARREGATRVLGVTVRLGPWSALSADHV
                     REHFSEAARGTIAEGARLVVSAAGDDVGPIERGEVLESIDIET"
     gene            complement(4441..4902)
                     /locus_tag="AC16BC_09455"
     CDS             complement(4441..4902)
                     /locus_tag="AC16BC_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR3241886.1"
                     /translation="MPATIIGVGNRWRSDDGLGLVVAERLAAARPLPGVVVRQEDASG
                     LADAWRGADVAVVVDAMASSDPPGTIRRFEVGDAPVPRDTLRCSTHGFGVAEAIELAR
                     SLGRLPSRLIVYGVAGERFDHGSDLSEPVARAVDAVIARILADTAGGDARA"
     gene            complement(4904..>5648)
                     /locus_tag="AC16BC_09460"
     CDS             complement(4904..>5648)
                     /locus_tag="AC16BC_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3241887.1"
                     /translation="NERDTGASPGETMIADLRARWMAGVLAVMVCGAGVPVEADAASR
                     TGSKAGAKQRAAQADHTALFDAAWSGQTETVRSLLARGVDVNARDAGEKTALMAAAVN
                     GYADTVKALLAAGADVHAQDEHGETALMNAAFNGFADIVTLLIERGADVNAARESGDT
                     ALMDASWNGDEIMVNTLLAAGANVNARTLDGDTALMMAASNGHADVVRSLLAMKAEVD
                     MENRYGDTALSLAADEGYLDVVKLLLKRR"
BASE COUNT          933 a         2161 c         1741 g          813 t
ORIGIN      
        1 gtgacgagcc tcgcccagcg gattgccgaa ccccgcgaca aacacctcgt cgatcaggcg
       61 gtgcatcaac cgcccgcccc cgccgtgcgc gagttgaatg cgatcggagg cgtcgatcgg
      121 aatcggacac gaccacgcga catcggcgtt tcggtcaacg gacatcatcg tctcccgcag
      181 gggcacggca tgccgtgccc ctacacatcg ccgctccgcg cgccaacccc gaacatcggg
      241 acaggccgtc gataccggta atacgcggcg cacgcgccct cggacgacac catgggcgcg
      301 cccagcggat ggtcgggcgt gcatcgcgtg ccgaacgccg cgcagtccgc cgggattttc
      361 gcgcccagca tgatctcgcc cgccacgcac tcgccggctt cctcggccga ccgcgacgcg
      421 aggggaaacc gtcggagcgc gtcgaaccgc gcatagcgcg gccgcaacac cagcccgctc
      481 tcggcgatgt ccccgatgcc ccgccacgtc cggctcgacg cctcgaacac ccggcggacg
      541 atctcctggg ccccgctgtt tccggttcgt cgcacggccc gcgcgtactg gttctcgaac
      601 tcggcgcgtc cctcctccaa ttgcttcacg cactggtaaa tgccctggag aatgtccaac
      661 ggctcgaacc ccgtgaccac gatcggcacg cggaaccggg cggccaacgg ctcgtactgc
      721 gtgtacccca tcaccgcgca cacgtggccc gcggccagaa acccgtcgac ccgattgccc
      781 ggcgattcga ggatcgaggc catcgcgggc ggcaccagaa catgggcgac cagcaccgaa
      841 aaattctcca cgccgtcccg ttcggcttga tacacggcca gggcgttggc cggcgccgtg
      901 gtttcgaatc ccaccgcgaa aaacacgacc tgccgatcgg ggaagcgccg ggccaacgcc
      961 agcgcgtcga gcggcgaata cacgatccgg acgtcgccgc cctccgcctt caccgacaac
     1021 aagtcccggc cggaacccgg cacgcgcaac atgtcgccga atgaacagac gatcgcgccg
     1081 tggcgggacc cgatctcgat cgccgcgtcg atcaacgcga ccggcgtcac gcagaccgga
     1141 cacccgggtc cgtgcagcaa cgtcacctgc tcgggcagca actcgtcgag gccgaaccgc
     1201 acgatcgcgt gcgtctgccc cccgcagacc tccatgacgg accacggcct ggtcgtgatc
     1261 cgccggatcg cgcccgccac gtcccgagcg gcgttggcgt cgcggtattc atcgagaaac
     1321 ctcatacccc cctccgaaca ggcgactgaa agaccccgcc acgtccttcg acaggctcag
     1381 gacgaacgga atgacacgtg atagtcaggc cgtttaccgt tcgcggcgag ctcggcgaac
     1441 cacgctcgac gtctcgtcac cggctaatcg cgctcgccga attctccgag ctcagcgata
     1501 tattccaatg cgcgcgcggc ttcggcctca tccacccgcg cgatcgcgaa ccccacgtgc
     1561 acgatcacgt aatcgccgac cgcggcctcg ggcacaaagg ccaaggacac gtccttcacg
     1621 atgccgccga actggacccg cccggcgcga tccactccct ccgcgccgtt gaccgccacg
     1681 atccgtcccg gaatcgcaag gcacatggtc gtctcagggc aaggagcgaa atccccccgc
     1741 cccccccttt tccaaagggg ggatgggagg aatgggattc gtccctgcat acgcgaccgc
     1801 ctgcccaagc gcaaggccgc cgtcgttggg cgggaccctg cgatgtcgat agaccgtcaa
     1861 acccgatgcc gtcagccgcg ccgtcgcgcg ttcggtcagg taacgattct ggaagcaccc
     1921 gccggtcagc gcgattcgcg gctcgcccac gcgctccgca accgtgacga tcgcctcgac
     1981 cagcgcctca tggaacctgc gtgccatgac tgaaacgacc acgccgcgcc ccacatcatc
     2041 gagtatcgcg cgcacggtgg gctcccaatc caccaccaga atattgtcgt gctccaacac
     2101 gctgatcggg tatggctcgc cggtgctacc taaatccccc cgttcccccc acgagtcaaa
     2161 tccccccatc cccccttttt caaagggggg cacgggggga ttttgctcag ggcgggacat
     2221 cggggggatg tgccccttct cggccgcaaa ctccaggtcc atcgccgcct gcccttcgaa
     2281 cgtcgccgtc tgcctgatcc cgatcaacga cgagaccgcg tcgaacaatc gtccaacgct
     2341 cgacgtgagc ggggcgttga ctgcccgcga cgccatgcga gcgagcaccg cgcgctcgga
     2401 cgcggaccac gaggcgagcg gcgggaggtc gtcgcgtgtc gccacgcgct ccccgaacag
     2461 ttcgaccagc actccgagcg cggcccggcg cggctcgcgg attgcctgct ctcctcccgg
     2521 caaacgaaac gtcctcaaat gcccgacccg tcgccacgac ccgtcatcaa caatcaggaa
     2581 ctccccgccc cagatcgtcc cgtcgagacc gtacccggtc ccgtcccaag ccacgcccag
     2641 caccgggccg tcgagtccgt gttccgcccg gcacgccagc acgtgggcgt ggtggtgttg
     2701 gatcccgacc acgggctgtc cgaatgacgc gctgtcgcgc gccgcggcat cgtccgggtg
     2761 acgatcgcac cgagtgaccg ccggggtgac cccgtgcagt cccgcaagat ccgatgccgt
     2821 gcgtcgatgg accgcccgcg tgtccgcggc gctcaggttg ccgatgtgct ggctcagcgc
     2881 cacggcctcg ctcgtgcgca gcgccacggt gttcttctgt ccgccgccca cggcgagcgt
     2941 cgaaacctcg gaccgcgcga ccgggatcac gagcggcgcg tagccccgcc cgacgcgcaa
     3001 gacgaccgcg gcgtccccga tgacgcggac cacggaatcg tcgacccggc gctcaatggc
     3061 gcggtcgtgc accaacaaca gatccgcgat cccgcggaac cgctccaccg cgtcgcgctc
     3121 ctcgatgcag atgggctcgt ctgagcgatt gccgctggtc gccaccaccg gagccttgac
     3181 cgcccgcgcc accaataggt gcaggggcga cggcggcagc atgacgccca ggttgggatt
     3241 gccaggcgca accgacgggg ccacggccga ggatcgcgcg cgaaccaaca cgatcggccc
     3301 ttccacggat tgcagcagta aacgctccgg ttcggcgacg tcgcaccacg cctcgaccgc
     3361 gtccagcgat tcgcagagca ccgcgaaggg tttgtcggga cggtgtttgc gccgtcgcag
     3421 ctcgcgcacc gcggaatcgt tcgtggcctc cgccagcagg tgaaacccgc ccacgccctt
     3481 gaccgcgaca atccggccgt tgcgaatcgc ggccgcggcc gcggccacgg cgtcgtatcc
     3541 cgtggactcg acccggccat cgttgtccca cgccgcgagt tgcggaccgc acgccgggca
     3601 cgcaatcgcc tcggcgtgga agcgacggtc cgagggatcg tcgtattcgg cccggcacgc
     3661 cgcgcacatc gggaaacgcg ccatggtcgt gttcgatcga tcgtagggca tggcccggat
     3721 gatcgtgtag cggggaccgc aggacgcgca atgggtgaag ggataccgat agcgccggtt
     3781 ggacggatca aggacgtcgc gaagacactc ggggcagggc attacatccg gcggaacatg
     3841 gacgccggcg gcgccctcgg agtccgacgg atgaatcgca aaccccgcga ccccgatcag
     3901 ttcgccggag gtcgcgcgca cgtcgtcgat cgcggcgtga ggcggggcct cggaccggag
     3961 tcgatggacg aaccggtcga gcgccgcggg ctcgccttcg gccgcgacgc gcaccccgcc
     4021 gcgttcgttt ttcacccacc cggacaagcc cagctccacg gccaatcgat acacgaacgg
     4081 ccggaagccc acgccctgga cgcggccgct caccagcacg tcgaatcgtc gcggctcgac
     4141 gcgcggcggg gtgacggcgg acctcgcttc aacggtgagc ggcgtcatgt ttcgatgtcg
     4201 atgctctcca acacctctcc ccgttcgatc ggaccgacgt cgtcgcccgc ggcgctgacc
     4261 acaagccgcg cgccctccgc gatcgtcccg cgcgcggcct cggagaaatg ttcccgcacg
     4321 tggtccgcgg acagcgccga ccacggaccg agccgcaccg tcacgcccag gacccgtgtg
     4381 gcgccttcgc gccgcgccac ggccgcgacc gtccgcagga ggtcatccac ccacgcggcg
     4441 tcatgcacgg gcgtcgcctc ccgccgtgtc cgcgagaatg cgcgcgatca ccgcgtcgac
     4501 ggcccgcgcc accggttcgc tcaagtcgct cccgtggtcg aatcgctcgc cggccacccc
     4561 gtacacgatc agcctcgacg ggaggcggcc cagtgatcga gccagctcga tcgcctcggc
     4621 cacgccgaac ccgtgcgtgg aacagcggag cgtgtcccga ggaacgggcg cgtctccgac
     4681 ttcgaatcgc cgaatcgtcc cgggcggatc ggacgacgcc atcgcatcga ccaccaccgc
     4741 cacgtcggcg ccgcgccacg cgtcggcaag cccggacgcg tcttcctggc ggacgaccac
     4801 gccgggcaac gggcgggccg cggccaatcg ctccgccacc accaggccca ggccgtcgtc
     4861 gctgcgccac cggttcccga cgccgatgat tgtcgccggc atgtcagcga cgcttgagca
     4921 gcaacttcac cacgtccaga tacccttcgt cggcggccaa gctgagcgcg gtgtcgccgt
     4981 accggttctc catgtcgacc tccgccttca tcgccagcag cgaccgcacg acgtcggcgt
     5041 gtccgttcga cgcggccatc atcaaggccg tgtcgccgtc gagggtccgc gcgttcacgt
     5101 tcgcgcccgc ggcgaggagc gtattgacca tgatttcgtc gccgttccac gacgcgtcca
     5161 tcagcgcggt atccccgctc tcgcgggccg cgttgacgtc ggccccgcgc tcgatcagga
     5221 gagtcacgat gtccgcgaag ccgttgaacg ccgcgttcat cagcgccgtc tcgccgtgtt
     5281 cgtcctgcgc gtgcacgtcg gcgcccgccg ccagcaacgc cttgaccgtg tccgcgtacc
     5341 cgttgaccgc cgcggccatc aacgcggttt tctcgcccgc gtcccgcgcg ttgacgtcga
     5401 cgccccgcgc cagcagcgac cgaaccgttt cggtctgccc gctccacgcc gcgtcgaaca
     5461 acgcggtgtg atccgcctgt gcggcccgtt gtttcgcgcc ggctttggat ccggtgcgcg
     5521 acgccgcgtc cgcctcgacc ggcacgcccg cgccgcacac catcacggcc agcactcccg
     5581 ccatccaccg cgctcgaagg tccgcgatca tcgtttctcc cggagaagcc cctgtgtcac
     5641 gttcgttg
//
LOCUS       DBNGTT010000422        12674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_868553, whole genome shotgun sequence.
ACCESSION   DBNGTT010000422 DBNGTT010000000
VERSION     DBNGTT010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_868553"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="AC16BC_09465"
     CDS             complement(<1..454)
                     /locus_tag="AC16BC_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241888.1"
                     /translation="MYRFIGMLTGVAVLALGGFAPALAAEYEETAVNNGGTITGTVTL
                     AGPIPEPRVFPLVLYPFGPFCKKISDGKGHIQVDEFIVSPDRGLQDTIVAVQRVTRGK
                     PFPRLKQELIAVDCMFHPAETAEDELFAIDAKGKLHHEHPLVMVLRNHE"
     gene            complement(535..5436)
                     /locus_tag="AC16BC_09470"
     CDS             complement(535..5436)
                     /locus_tag="AC16BC_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3241889.1"
                     /translation="MRTKVWGVVWGLALGAWLSGGPPAARAGDEHGAHDAASGTTVMA
                     AAQELSSWEQKFKQQLEREDAAEGRNGHRDQVEAAMKKLMSEIAGGTTSHGSHTAQGP
                     YSDAAMAQQMDKSYFLGVASTAETVTQGGHCPANVPTKEYDVSAINVEITLNQWLDYH
                     PGYMYVLTENIQKVRDEEKANREARKAVGYNPGAVTTGMQTDMIQPIVIRGNQGDCVQ
                     ITLRNQLDGEDVGLHIHGSSMIIKATGQAATASNADSTVKPGKSQAFEWYIRPGEQEG
                     SHMLHSHVGREQASLGMIGAFIVEPMGSRYLDPITGKEAKSGWQMMIEDPNAPDFREY
                     AIFYHEIGDESFRPLNKQGDTIPQRDPNTDAYRPSARALNYRSEPFGINNLALQEKYF
                     HFEDESMAYSSYTFGDVPTPIPRGYLGDPNKWRLIHGGGEVFHSHHPHGGSIRWLRQP
                     KADGKADFLMTAAQNGPVKYPEVRTTSDRVDVEVIGPSEVLDLQTECGWGLCQQVAAD
                     YLFHCHVAHHYVAGMWSYGRGYNTLQTGNYPVGSTDVMPSLQELPDRKGRMKPAVTSD
                     QLVGKTMDWYDKKWNITADKTDFSKPIPDVSIKDWVKMMIPPAGQPGHTADEKGQIEA
                     YDATVWDWTWDGNKAMGEPEATQTFDNPKYKSPIPGKRPPILFDAKTGKLAFPQFKPH
                     FGKRVPFARHHGPAPWLEPIHMDKNTGSVGDEPGGIGVAGGETNQPARPGEQGKWSLC
                     PQNAGRKQYTIHFIETPITMNNAIGKEKAVVDQHGTIYVLHEEEASVRSNSDLARPLV
                     YRANVYDCVDVILKSEWLDNDTTNFQMSKINIHPHFMQFDNNSSDGVITGFTYEMSVR
                     PFTQFKKDKAKGLPVPMNAHLTEDVKAGATAIKIKMPEGATKFHVGTDIMVGMEEVKT
                     SEVRWIKDIQGDTLVFSEPLKHAHKKDEIASVEFVRYRYWVDADLGTVFWHDHALGGT
                     TWPHGAVGGLIVEPVGATYHDPKTGKEIRSGPIADIHSTDPIGYGVNGSFREMVQYLH
                     DTVPYTAQVVTAGNPPGQTVKAAIDAGQALFFQMPYDLDLVSVPMLNGGTHTTGGGFF
                     FKAESIAKRLKNDPDPSHVFSTRAHGKDPGTPLLRAYLGDTVVFRLMHVMMNESHTWH
                     LAGHAFRTERYAEKSDFKNAWHVGIAERYDLVTKAGGPQQMAGDYLHFDGRPSHLSEG
                     SWGIFRVLDKEVPDLKKLPGRDEIPASAKSVCPADAPVKTFNVIAADRALKYNSHAPD
                     VIEVDFDRTLRVANPKGKIYMLEGEKSKVAEGGAQPMPLTLHVNVGDCIKVNLKNEMA
                     TSRASFSADMLAFAPNDSQGVNVGNNPGDQTVAPGKSRTYTFYAPPQYGETAAIVWDW
                     GNFVNNVRDGLFGGIIVGPKGSKYRDPKTGEDVSMKNAWQVDVIVDRSIPENANRSDF
                     RDASLFFQDEDNIIGTAFMPYIQQIAGLTGVNYRSEPWTFREENGCELGNMFTPCVAA
                     ETELVTPTIMAHAGDPVRLHVFGAFNEQNQVFSLEGHEWPLKPNMEGADMMSSSEFGS
                     SQNLDVLLKDGAGGPFRLPGDYLWQNHRMPYTQAGQWGYLRVLPAGDRRLLPLNSGGP
                     GGRTAELPRDNSEVAVSMLHPEK"
     gene            complement(5433..5825)
                     /locus_tag="AC16BC_09475"
     CDS             complement(5433..5825)
                     /locus_tag="AC16BC_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241890.1"
                     /translation="MAAKTRVLVVGSGALFGAAVEGLLGAQRGDQVEVESVRTVEAAI
                     EVAPRFQPDVIVFCLERDDPRDHNAWQRLRLMEVCPARVIRCTLESNHLTIYDTTRIV
                     DATAEDLLSAVCDTAGSPAATSRKGEEA"
     gene            complement(6027..6443)
                     /locus_tag="AC16BC_09480"
     CDS             complement(6027..6443)
                     /locus_tag="AC16BC_09480"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3241891.1"
                     /translation="MTEPGAVVTWQGGVRFDADVRGHRIVIDQPKDEGGSDEGMTPLE
                     CLGVSLGSCVAFFAARFAQRHAVSLADLRVGVTWDYAEQPHRIGRFDVRVTCRGELHA
                     AMTERLRRVVEGCTVHHTLTHAPDIKVTLTVGESAA"
     gene            complement(6440..6646)
                     /locus_tag="AC16BC_09485"
     CDS             complement(6440..6646)
                     /locus_tag="AC16BC_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR3241892.1"
                     /translation="MNYNVGNVDRAVRGVIGIALAAGALNAEAPLVRAVVGVLALVMV
                     GTALVGVCPLYQVFGLNTGRRKPR"
     gene            complement(6678..7649)
                     /locus_tag="AC16BC_09490"
     CDS             complement(6678..7649)
                     /locus_tag="AC16BC_09490"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HIR3241893.1"
                     /translation="MRRIGVLTSGGDAPGMNAAIRAVTRTGIAAGLEVVGIRRGFAGL
                     IAGEPGALTARSVSGIIDRGGTMLGSARSPEFTTEDGQRRALATIRAAALDALVVIGG
                     NGSQAGAYALEKLGVPVVGVASTIDNDLEGSDITIGVDTALNVALEAIDRIKTTAASH
                     RRAFLVEVMGRDCGYLALMAGIAGGAEVVAIPEQACDPEAMAAQFRAAYDGGKAHAIG
                     VIAEGVKFRAADIAEYFRAHHERIGFDLRVTCLGHIQRGGAPGAFDRLLATRLGAGAV
                     DALVRGRHGVLVGFVRGEVATTPLAEVVASCKPLDMRLWDLAKVLAK"
     gene            complement(7932..9560)
                     /locus_tag="AC16BC_09495"
     CDS             complement(7932..9560)
                     /locus_tag="AC16BC_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR3241894.1"
                     /translation="MERKPFAITLDPSSSLANHTGNWRTKRPVYVSRLAPCNHACPAG
                     EDIQRWLYPAEEGRYEDAWRELVKDNPMPGIHGRVCYHPCESACNRAQLDEAVSIHAV
                     ERFLGDLAIKNGWRVEAAAATGRRVLVVGAGPSGLSAAYHLARMGHTVEVFDAGPKAG
                     GMMRFGIPKYRLPREVLDAEVARIEGLGVRFVLNRRVADLAATMGEGAFDAVFLAVGA
                     QIGRRVDIPARDARRLVDAVGFLRAVESGAPIRLGRRVAVYGGGNTAVDAARTARRLG
                     AEETVIIYRRDRAHMSARDFEVAEALEEGVAIRWLESIAGFGDGRLRVEAMELDANGR
                     PVPTGRFEELAADTVVLAVGQEVDTTFLRRVEGLVIRPDGVIAVNDAMMTGHPGVFAG
                     GDMVPSDRTVTASTGHGKLAARHIDAWLKGTRYRPPDPQPPATFDRLNTWYYTDAEQA
                     KQPVLAPAERRAGFDEVVGGLDEATALLEARRCLSCGNCFRCDNCYGVCPDNAVIKLD
                     PAKGYAIDYDYCKGCGMCAQECPSGAIDMIDEIV"
     gene            complement(9550..10560)
                     /locus_tag="AC16BC_09500"
     CDS             complement(9550..10560)
                     /locus_tag="AC16BC_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3241895.1"
                     /translation="MSSERIKFYQTGTFTVGNRLLSEEERTVQSDVRRTNSLTSGHRA
                     CQGCGEALGARYAVDAAMRAANNRLIAVNATGCLEVFSSPYPESSWRIPWLHSLFGNA
                     PAVASGVAAALKAKGRADTLVLAQGGDGGTVDIGFGCLSGMFERNDDVLYVCYDNEAY
                     MNTGVQRSGSTPPAARTMTTPVTAGRPGNDFGTGKNVPKIAMAHGIPYVATASVADLH
                     DLEAKVMKAARIRGARYIHVFVPCPLGWAHDPALTVTVARLAVECGLFPLFEAEFGRV
                     TGRTTIRRRVPVERYLELQGRFKHLFGDPPNREALAAIQAIADRQIEEYGLLGEGAAH
                     GA"
     gene            complement(10557..11822)
                     /gene="porA"
                     /locus_tag="AC16BC_09505"
     CDS             complement(10557..11822)
                     /gene="porA"
                     /locus_tag="AC16BC_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HIR3241896.1"
                     /translation="MLKQIEGSQAIAAAVAACRPEVISCYPITPQTHIVEALSSMVRR
                     GEIGKCQFLTVESEFAALSALIGASATGARTYTATSSQGLLFMAEAVYNASGLGLPVV
                     MTIGNRAIGAPINIWNDHSDSMALRDAGWIQLHAESNQEAVDLHIQAFRLAEALGVPV
                     MVCVDGYVLTHAFEAVDVPEQALVDGYLPPYEPVQLLDPDHPVTVGAMVGPEAFTEVR
                     YLAHRVQLRALDLIPDLAAEFAEDFRRRSGGLLVPYRTEGAETIVVALGSVNGTIKDT
                     VDVLREGGGSVGCVKLVSYRPFPGEALRNAVAGARRVIVIEKDLAVGIGGIVSADVKL
                     ALSGTGVAVHTVIAGLGGRPITGASLRGVIERGRDDVLGEPHFLDLNEGVVDREIARQ
                     RERRRSGPTALNVLRDAGGPSAHGGRGAP"
     gene            complement(11806..12414)
                     /locus_tag="AC16BC_09510"
     CDS             complement(11806..12414)
                     /locus_tag="AC16BC_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004011387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3241897.1"
                     /translation="MFQVRIHGRGGQGVVTAAELLSQAAFRDGLHAQAFPSFGSERMG
                     APVVAFCRINDRAIRSREPVTHPDMVVVQDATLLHHVDLFAGLSLSGYVLLNSSRTSA
                     ELGIADLVGRLPAGRVVTVPATDIARRHTGRPLANAALLGAIAALTGVVSLRAVGLAV
                     QERFPCRVGTANAAAAEAVFEAVQTRDTVSTPVRGGAGAQAN"
BASE COUNT         2108 a         4467 c         3786 g         2313 t
ORIGIN      
        1 gttcgtgatt tctcagcacc atgaccaagg gatgctcgtg gtgcagcttg cccttggcgt
       61 cgatcgcaaa cagctcgtcc tccgcggtct cggccggatg gaacatgcag tccaccgcga
      121 tcaattcctg tttgagacgc gggaatggtt tgccgcgcgt gacccgttgc accgccacga
      181 tcgtgtcctg caggccccga tccggggaca cgataaactc gtccacctga atatggccct
      241 tgccatcaga aatcttcttg cagaagggac caaagggata cagcaccagg gggaagaccc
      301 tcggttccgg aatcggcccc gccaacgtca cggtccccgt gatcgtcccg ccgttgttta
      361 cggccgtctc ttcatactcc gccgccaagg ccggtgcaaa tcctcccaac gccagcacgg
      421 ccactcccgt caacataccg atgaaccgat acatcgcgct cccttcgctg acacaacggg
      481 gcaggggaca tcagcgtccc ctgccccgcc cagtctactt cgttccgact cccattactt
      541 ctccggatgc aacatcgaaa cagccacttc gctgttgtcc cgcgggagtt ccgcggtccg
      601 accgccgggc ccgccgctgt tcagcggaag gagtcgccga tcacccgcag gcaacacccg
      661 cagatacccc cactgtccgg cctgggtata gggcatccgg tgattctgcc agaggtaatc
      721 ccccggaaga cggaatggcc cgccggcacc gtccttcagg agcacgtcca gattttgcga
      781 tgacccgaac tcactcgagc tcatcatgtc ggccccctcc atgttcggct tgaggggcca
      841 ctcgtgacct tcgaggctga agacctggtt ctgctcgttg aacgcgccga acacgtggag
      901 cctcaccgga tcaccggcgt gcgccatgat ggtcggcgtc accagctcgg tctccgcggc
      961 cacgcacggc gtgaacatat tgcccagttc gcatccgttc tcctcccgga aggtccacgg
     1021 ctcggaccgg taattcacgc cggtcagtcc cgcgatctgc tggatgtacg gcatgaacgc
     1081 ggtgccgatg atgttgtcct catcctggaa gaagagcgag gcgtcccgga agtcggagcg
     1141 attggcattc tccgggatcg accggtccac gatcacgtcg acctgccacg cattcttcat
     1201 cgagacgtct tcgccggtct tcggatcccg atacttcgac cctttcgggc ccacgatgat
     1261 ccccccgaac aaaccgtccc ggacgttatt gacgaaattc ccccagtccc acacgatcgc
     1321 cgcggtctcc ccgtattgcg ggggcgcgta gaaggtgtag gtccggctct tccccggcgc
     1381 cacggtctga tcgccggggt tgttgcccac gttgaccccc tgcgaatcgt tcggggcgaa
     1441 cgccagcatg tccgcgctga acgacgcgcg gctcgtcgcc atctcgttct tcaagttgac
     1501 cttgatgcaa tcccccacat tgacgtgcaa cgtgagcggc atgggctgtg cgccgccctc
     1561 cgccaccttc gacttctccc cttcgagcat atagatcttg cccttggggt tggccacccg
     1621 gagcgtgcgg tcgaagtcca cctcgatcac gtcgggcgca tggctgttgt acttcaaggc
     1681 acggtccgcc gcgatcacgt tgaaggtctt gaccggcgca tccgccgggc agaccgattt
     1741 ggccgacgcc ggaatctcgt cgcgcccggg cagcttcttc aagtccggca cttctttatc
     1801 caacacacgg aatatgcccc aactcccttc ggacagatgg gacggccggc cgtcgaagtg
     1861 caggtagtcc ccggccatct gttgcggtcc gccggccttg gtcaccagat cgtaccgctc
     1921 cgcgatgccg acgtgccacg cgttcttgaa gtccgacttc tccgcgtacc gctcggtccg
     1981 gaaggcgtgg ccggccagat gccacgtgtg ggactcgttc atcatcacgt gcatcaagcg
     2041 aaacaccacc gtatcgccca ggtacgcccg caacaacggc gttcccgggt ctttcccgtg
     2101 ggccctggtg gaaaacacgt gcgacggatc cgggtcattc ttcagccgtt ttgcgatcga
     2161 ctcggccttg aagaaaaacc cgccgcccgt ggtgtgtgtc ccgccgttga gcatcggaac
     2221 ggacaccaga tccaggtcat acggcatctg gaagaacaac gcctgaccgg cgtcgatggc
     2281 cgccttgacc gtttgaccgg gcggattccc cgcggtcacg acctgcgcgg tgtacggcac
     2341 ggtgtcatgc aggtactgca ccatctcccg gaagctcccg ttgactccgt atccgatcgg
     2401 atcggtggaa tgaatatcgg cgatcggacc gctccggatc tccttgccgg tcttgggatc
     2461 atgatacgtg gcgccgaccg gctcgacgat gagccctccg accgcgccat gcggccaggt
     2521 ggttccgccg agcgcgtggt cgtgccagaa cacggttccc aaatcggcgt ccacccagta
     2581 gcggtaccgg acgaactcga ccgacgcgat ctcatctttc ttgtgcgcgt gtttcaaggg
     2641 ctcgctgaag acgagggtgt cgccctgaat atccttgatc cagcggacct cggacgtctt
     2701 gacttcctcc atgccgacca tgatgtcggt gccgacgtga aatttcgtcg ccccttccgg
     2761 catcttgatc ttgatggcgg tcgctccggc tttgacgtcc tccgtcaggt gggcgttcat
     2821 cggcaccggc aaccctttgg ccttatcctt tttgaactgc gtgaacggcc ggaccgacat
     2881 ttcatacgtg aatccggtga tcacgccgtc gctggagttg ttgtcgaact gcatgaagtg
     2941 cgggtggatg ttgatcttcg acatctggaa gttcgtggtg tcgttgtcca accactcgct
     3001 cttcaagatc acgtccacgc agtcgtatac gtttgcgcgg tagaccaacg gccgcgccag
     3061 atcactgttg gacctaacgg acgcttcttc ctcgtgcagg acatagatcg tcccgtgttg
     3121 atccaccacc gccttctctt tcccgatcgc attgttcatc gtaatcgggg tttcaatgaa
     3181 gtggatcgtg tactgcttgc gccccgcgtt ctgcgggcac agactccatt tcccttgctc
     3241 acccggccgg gccggctggt tggtctcccc gccggccacg ccgataccgc ccggttcgtc
     3301 gccgaccgag ccggtattct tatccatatg aatcggttcc aaccacggcg ccggtccgtg
     3361 atgacgggca aagggaaccc gcttcccgaa gtgcggcttg aactgaggga acgccagctt
     3421 cccggtcttt gcgtcgaaca aaatcggcgg ccgcttcccg ggtatcggcg acttatactt
     3481 gggattgtcg aaggtctgcg tcgcctccgg ctcacccatc gccttgttgc cgtcccacgt
     3541 ccagtcccac accgtggcgt catacgcctc gatctggcct ttctcatccg cggtgtggcc
     3601 cggttggccc gccggcggga tcatcatctt cacccagtcc ttgatggaca cgtccgggat
     3661 gggcttggaa aagtcggtct tgtccgcggt gatgttccat ttcttgtcgt accagtccat
     3721 ggtcttgccg accaactggt ccgacgtcac cgccggcttc atccggcctt tccggtccgg
     3781 caactcctgc agcgacggca tgacatccgt gctgccgacc ggataattcc cggtctgcag
     3841 cgtgttgtag ccgcggccat aggaccacat cccggccaca tagtggtgcg cgacgtgaca
     3901 atggaagagg tagtccgccg cgacctgctg gcagaggccc catccgcact cggtctgaag
     3961 atccaacacc tccgacggac cgatcacttc gacgtcgacc cggtccgagg tggtcctgac
     4021 ctccggatat ttcacgggac cgttctgcgc cgcggtcatc agaaagtccg ccttgccgtc
     4081 ggccttgggc tgacgcagcc agcgaatcga cccgccgtga ggatgatgcg aatgaaacac
     4141 ctcgcctccg ccgtgaatca ggcgccactt gttcggatcg cccaggtacc ctctcggaat
     4201 cggagtcggc acgtcgccga aggtgtacga gctgtaggcc atggactcgt cttcaaaatg
     4261 gaagtacttc tcctgaagcg ccaagttatt gatgccgaac ggttcgctgc gatagttgag
     4321 cgcccgtgcc gacggacggt acgcgtccgt gttgggatcg cgctgcggga tcgtatcgcc
     4381 ctgtttgttc aacggccgga aggattcatc gccgatttcg tgatagaaga tcgcgtactc
     4441 ccggaaatcc ggcgcgttcg ggtcctcgat catcatctgc cacccgctct tggcctcttt
     4501 cccggtgatc ggatcgaggt agcgggaccc catgggctcg acgatgaacg cgccgatcat
     4561 gcccaggctc gcctgctcgc gccccacgtg gctgtggagc atatgcgaac cttcttgctc
     4621 gccgggccgg atgtaccact cgaacgcctg actcttgccc ggcttgaccg tggagtccgc
     4681 gttggaggcg gtggcggctt gtccggtggc cttgatgatc atgctggaac cgtggatatg
     4741 aagcccgacg tcttcgccgt ccagctgatt gcgcagggtg atctgcacgc agtcgccctg
     4801 gttcccccgg atcacgatcg gctggatcat gtcggtctgc atgccggtgg tcacggctcc
     4861 ggggttgtat cccaccgcct tccgggcctc acggttggcc ttctcttcat cccgaacctt
     4921 ttggatgttc tcggtcagca cgtacatgta gccgggatga tagtccaacc attggttcaa
     4981 cgtgatctcc acgttgatcg ccgacacgtc gtattccttg gtggggacgt tggccggaca
     5041 atgaccgccc tgcgtcacgg tttcggccgt cgacgccacg cccaggaaat agctcttgtc
     5101 catctgctgg gccatggccg cgtcgctgta cgggccttgc gcggtgtggc tcccgtgcga
     5161 cgtggtgccg cccgcgatct ccgacatcaa cttcttcatc gcggcttcca cttggtctcg
     5221 gtggccattg cggccttcag cggcgtcctc gcgttcgagc tgctgcttaa acttctgttc
     5281 ccacgaagac agctcttgag cggccgccat caccgtcgtt cccgaagccg cgtcgtgcgc
     5341 gccgtgttcg tcaccggccc gcgccgctgg cggaccgccg gacaaccagg cgcccagcgc
     5401 cagtccccac actacgcccc acaccttcgt cctcatgctt cctccccttt tctactcgtg
     5461 gcggctggac tgccggcggt gtcacacacc gccgagagca ggtcttccgc cgtggcgtcc
     5521 acaatccgcg tcgtgtcata gatggtgaga tggttgcttt cgagcgtgca gcggatcacg
     5581 cgcgcgggac agacttccat cagccgcagg cgctgccacg cgttgtggtc gcgcggatca
     5641 tcgcgctcca ggcagaacac gatgacatcc ggctggaatc gcggcgccac ctcgatggcc
     5701 gcctccacgg tccgcacgga ttcgacctcc acctgatcgc cgcgctgcgc ccccagaagg
     5761 ccttcgaccg ctgctccgaa cagcgcccca ctcccgacca ccaacactct cgttttcgcc
     5821 gccatggtct cctcactcgg cgcggaccgt gtcctgccgc gtacgatagc gtttcaccac
     5881 cgggccgtcg attcttcgga gtaagtattt ttgttaggcc tattgggcca atggggagta
     5941 gatgaatcga acgacgttgt ccgtcggcgc ctcatcacga tcgtgatatc cctccaatgg
     6001 gtcatacgat cgacgcgagc ggcccgtcac gccgcggact cgccgaccgt gagcgtcacc
     6061 ttgatatccg gcgcgtgcgt cagggtgtgg tgcacggtac agccctccac cacgcggcgc
     6121 agacgctcgg tcatcgccgc atgtaactcg ccgcggcacg taacccgcac gtcgaatcgc
     6181 ccgatgcggt ggggctgctc cgcgtagtcc cacgtgaccc cgacccgaag atcagccagc
     6241 gacacggcgt gccgctgcgc gaaccgggcc gcaaaaaacg ccacgcaact ccccaacgac
     6301 accccgaggc attccaacgg cgtcatgcct tcgtcgcttc cgccctcgtc cttgggctga
     6361 tcgatcacga tccggtggcc gcgcacatcc gcgtcgaacc gaaccccgcc ttgccacgtc
     6421 acgaccgcgc cgggttcggt catcggggtt tccgccgacc ggtgttgagt ccgaacacct
     6481 gatagagggg acagaccccc acgagcgcgg tccccaccat gaccagcgca aggacgccaa
     6541 caaccgctcg gaccaacggc gcctccgcgt tgagcgcgcc cgccgccagc gcaattccga
     6601 ttaccccacg cacggcccga tcgacattgc ctacgttgta gttcatctcc gattcccccc
     6661 gtctccccct cgtcccttca cttcgccaga actttggcca gatcccacag ccgcatgtcg
     6721 agcggcttgc aggatgcgac cacctccgcc agcggcgtag tcgcgacctc gccgcgaacg
     6781 aacccgacca atactccgtg ccggccgcgc accagcgcgt caacggctcc ggcgcccaga
     6841 cgcgtggcga gcaggcgatc aaacgcgccc ggcgcgcccc cgcgctggat gtgtcccaaa
     6901 caggtcacgc gcagatcgaa cccgatgcgc tcgtggtggg cgcgaaaata ctcggcgatg
     6961 tccgcggcgc gaaacttgac cccctccgcg atcacgccga tggcatgcgc cttgccgccg
     7021 tcgtacgccg ccctgaactg cgcggccatg gcctccgggt cgcacgcttg ctccggaatc
     7081 gccaccactt cggccccgcc cgcgatgccg gccatgagcg ccagataccc gcagtcgcgg
     7141 cccatgacct ccaccaggaa cgcccgccgg tgggacgccg cggtggtctt gatccggtcg
     7201 atcgcctcca gcgcgacatt gagcgcggtg tcgactccaa tggtgatgtc gctgccctcc
     7261 agatcgttgt cgatggtgga cgccacgccc accaccggca cgcccagctt ttccaatgcg
     7321 tacgcgccgg cctgtgaccc gttcccgccg atcacgacca gcgcgtccag cgccgcggcc
     7381 cggatcgtgg ccagcgcccg gcgctggccg tcctccgtgg tgaattccgg gcttcgcgcg
     7441 ctgcccaaca tggtgccccc gcggtcgatg atcccgctca cggatcgcgc ggtgagcgcg
     7501 cccggctccc ccgcgatgag gcccgcgaac ccgcgacgga tccccaccac ttccagcccc
     7561 gcggcgatcc cggtccgcgt gacggcgcgg atcgccgcgt tcatgccggg agcgtcgccc
     7621 ccgctggtca gcactccgat tcgtcgcatt accccctcct tcccagaaga ttactgcgcg
     7681 agtatcggct aatcgatgct ccgagggaag taccgcggca gatttgtggg gggagcgcct
     7741 tacccaaagg ggtaaggcgt aagctttggg actgattatt tcagggttgc ccgcgtatcc
     7801 ttatacgcgg agatcaatca ggaatggaca gatgcaaggc gctgcgagca ccggagcgga
     7861 gcgtactccg tgtgtacgtg agcaccggat gcgcagcagc aacgcggcag atggccgttc
     7921 ctggttgatc tctagacgat ctcgtcgatc atgtcgatcg cgccgctcgg acactcctgc
     7981 gcacacatgc cgcagccctt gcaatagtcg tagtcgatcg cgtacccctt cgcagggtcg
     8041 agcttgatca ccgcattgtc gggacagacc ccgtaacagt tgtcgcagcg aaaacagttc
     8101 ccgcaggaca ggcagcggcg ggcctcaagc agcgcggtgg cttcgtccaa gccgcccacc
     8161 acttcgtcga agcccgcccg ccgttcggcc ggcgcgagca ccggctgttt ggcttgctcc
     8221 gcgtcggtgt aataccacgt gttcaggcgg tcgaacgtgg ccggcggctg gggatccggc
     8281 ggccggtatc gcgtcccctt gagccatgcg tcgatgtgcc gcgccgcgag tttgccgtga
     8341 ccggtcgagg cggtcacggt ccggtcggac gggaccatgt ccccgcccgc aaacacgccc
     8401 ggatgcccgg tcatcatggc gtcgttcacc gcgatgaccc cgtccggccg aatcaccaat
     8461 ccctccacgc ggcgcagaaa cgtggtgtcg acctcctgcc ccacggccaa caccacggtg
     8521 tccgccgcga gttcctcgaa ccggccggtc ggaacgggcc ggccattcgc gtccaactcc
     8581 atggcctcga cgcgcaaccg tccgtcccca aaccccgcga tcgactccag ccagcggatc
     8641 gcgacgcctt cttcaagcgc ctcggccacc tcgaagtctc gtgccgacat gtgcgcgcgg
     8701 tcgcgccgat agatgatgac cgtttcctcg gcgccgagcc gcctggcggt tcgcgccgca
     8761 tccaccgcgg tgttgccgcc cccgtacacg gccacgcggc ggccaagccg gatcggcgct
     8821 cccgactcga ccgcgcggag gaagccgacc gcgtccacga ggcgtctggc gtcgcgggcc
     8881 gggatgtcca ccctccggcc tatctgcgcg cccaccgcca ggaacacggc gtcgaacgcg
     8941 ccctccccca tggttgcggc gagatcagcg acccgacgat tgaggacgaa ccggacgcca
     9001 agcccctcga tccgggcgac ctccgcgtcg agcacctcgc gcggcaaccg gtatttggga
     9061 atcccgaagc gcatcatgcc gccagctttc ggtcccgcgt cgaagacctc caccgtgtgg
     9121 cccatccgcg ccaggtggta cgcggcggac aatccgctcg gcccggcgcc caccaccaac
     9181 acccgccgac ccgtggccgc agcagcctcc acgcgccaac cgttcttgat cgcaagatcg
     9241 cccaaaaacc gctccaccgc gtggatgctc acggcctcgt cgagttgggc ccggttacac
     9301 gcgctttcac acgggtggta acagacccga ccgtggatcc cgggcatggg gttgtccttc
     9361 accagttcgc gccacgcgtc ttcgtagcgg ccctcctccg ccggatacag ccaccgctgg
     9421 atgtcctccc cggccgggca cgcgtggttg cacggagcca ggcgcgacac gtacacggga
     9481 cgcttggtcc gccagttgcc cgtgtggttg gccagactgc tgctggggtc gagcgtgatg
     9541 gcgaagggct tacgctccat gggctgctcc ctctcccaac aaaccgtatt cctcgatctg
     9601 gcggtcggcg atcgcttgaa tcgccgcgag cgcttcgcgg ttcgggggat cgccgaacag
     9661 gtgcttgaag cgcccctgca attccaggta ccgctccacc ggaacgcgcc ggcggatcgt
     9721 agtcctaccg gtgacgcgcc cgaactcggc ctcgaacagg ggaaacaggc cgcattcaac
     9781 cgccaaccgg gctacggtga cggtcagcgc cgggtcgtgg gcccacccca acggacaagg
     9841 aacgaacacg tggatgtacc gcgcgcctcg aatgcgcgcc gccttcatca ccttggcttc
     9901 caggtcgtgc aaatccgcca ccgacgcggt cgccacgtac gggatgccgt gcgccatggc
     9961 gatcttgggg acgttcttcc cggtgccgaa gtcgttgccc ggccggccgg cggtcaccgg
    10021 ggtcgtcatc gtgcgcgccg cgggcggcgt ggagcccgag cgctgcacgc ccgtgttcat
    10081 ataggcctcg ttgtcgtagc agacatacag cacgtcgtcg ttgcgctcga acatcccgga
    10141 caaacatccg aacccgatgt ccacggtgcc gccgtccccg ccctgggcca acacgagcgt
    10201 atcggctcgc cccttggcct tgagcgcggc ggccactccc gaagccacgg ccggcgcgtt
    10261 gccgaacagc gagtgcagcc aggggatgcg ccaggagctt tccggatacg ggctcgaaaa
    10321 gacttcaagg catccggtcg cattgaccgc gatcaagcgg ttattggccg cgcgcatcgc
    10381 cgcgtccacc gcgtaccggg cgcccagcgc ttccccgcag ccctggcacg cccggtgtcc
    10441 ggacgtgagc gagttggtcc gtcgcacatc ggattgcacg gtccgctcct cctcggacag
    10501 gagccgattg ccgaccgtga aggtcccggt ctgatagaac ttgatgcgct cggaactcat
    10561 ggcgcgcccc gtcctccgtg cgcactcgga ccgccggcgt cacgcagcac gttcagcgcg
    10621 gtcggacccg accgccgccg ctcgcgctgc cgcgcgatct cccgatccac cacgccctcg
    10681 ttgagatcga ggaaatgcgg ctcccccaac acgtcgtcgc gcccccgctc gatgacgccg
    10741 cgaagggacg cgccggtgat cggccggccg ccaagccccg cgatgaccgt gtgcacggcg
    10801 acgcccgtcc cgctcagcgc gagcttcacg tcggcggaga cgatcccgcc gattcccacg
    10861 gcgaggtctt tttcaatcac gatgactctc cgagcgcctg ccaccgcgtt gcgcaacgcc
    10921 tcgcccggaa acggacgata cgagacaagc ttcacgcacc ccacggagcc gcccccctcg
    10981 cgaagcacgt cgaccgtgtc cttgatcgtc ccgttcaccg agcccaacgc caccacgatt
    11041 gtctccgcgc cctcggtccg atacgggacc agcaaccccc ctgaacgtcg tcggaaatcc
    11101 tcggcaaact ccgcagccag atccgggatg aggtcgagcg cccggagctg cacgcggtgc
    11161 gcgaggtagc ggacttcggt gaacgcctcg ggacccacca tggctccgac ggtcacgggg
    11221 tgatcgggat ccagaagctg gaccggctcg tacggaggca ggtacccgtc aaccagggcc
    11281 tgctccggca cgtccacggc ctcgaaggcg tgggtcaaca cataaccgtc cacgcagacc
    11341 atcaccggca cgccgagcgc ttccgcaagg cgaaaggctt ggatgtgcag gtcgaccgcc
    11401 tcttggttgg actcggcgtg caattgaatc caccccgcgt cccggagcgc catgctgtcc
    11461 gagtgatcgt tccagatgtt gatgggcgcg ccgatcgcgc gattcccgat ggtcatgacg
    11521 accggcagtc cgagacccga cgcgttgtac acggcttcgg ccatgaagag caggccctgg
    11581 ctgctggtgg cggtgtaggt tcgagccccg gttgccgacg ccccgatcaa cgcgctgagc
    11641 gcggcgaact cggactccac ggtcagaaac tggcatttac cgatctcccc gcgccggacc
    11701 atcgacgaca aggcttcaac gatgtgcgtt tgcggcgtaa tgggataaca ggaaatcacc
    11761 tcgggtcggc acgcggcgac ggcggcggca atggcctgcg agccttcaat ttgcttgagc
    11821 accagcgcct ccccttacgg gcgtcgatac cgtgtcccgg gtttgcaccg cctcgaatac
    11881 cgcctcggcc gccgccgcat tggccgtgcc cacgcgacat ggaaaccgct cttgaacggc
    11941 caggcccacg gcgcggagac tgaccacccc ggtgagcgcg gcgatcgcgc cgagcagcgc
    12001 cgcattggcc agcggccgcc cggtgtgccg tctggcaatg tccgtggccg gcacggtcac
    12061 gacgcgcccc gcgggaaggc gtccaaccag gtcggcaatc cccagttccg cggaagtgcg
    12121 gctggagttc agcagaacgt acccggaaag cgacagccct gcgaacaagt ccacgtggtg
    12181 cagcaacgtg gcatcctgca ccaccaccat atcggggtgt gtgaccggct cgcgcgaccg
    12241 gatcgcgcgg tcgttgatcc ggcaaaacgc cacaaccggc gcgcccatcc gctcggagcc
    12301 gaagctgggg aacgcctggg cgtgcagccc atcgcgaaac gcggcctgcg agagcaactc
    12361 agccgccgtc accacccctt gcccgccgcg accgtgaatg cggacttgaa acatcccgcc
    12421 tcctcgcgct gaacgcgagg ccacggtatc acatggggct agtgatcagg atcgctcgaa
    12481 atggttaggt ttgcgtcaat ttcggggtgg ggcgacgctg ggagaggcgg ttgggtgggc
    12541 ggaaaagccg ttgattcggt gatcgccgat cgcgttccgc cggcgcgtca gtggtgcgcg
    12601 tgttcccgtc gcacgcaata cggcccctcg cagccggtca gatccactgt cgggtcgcgc
    12661 tgcggcggaa tccg
//
LOCUS       DBNGTT010000423         1629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2018347, whole genome shotgun sequence.
ACCESSION   DBNGTT010000423 DBNGTT010000000
VERSION     DBNGTT010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2018347"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="AC16BC_09515"
     CDS             complement(<1..604)
                     /locus_tag="AC16BC_09515"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIR3241898.1"
                     /translation="MVREELFGPAPKRWEYTTLGIACERGGGDIQTGPFGSQLHAADY
                     VAEGIPSIMPQNIGDNRILEDGIARITPDDAERLSRYLVRKGDIVYSRRGDVERRALV
                     REKEDGWLCGTGCLRVRLGESGVDPRYAAFYLGHPSVREWIVRHAHGATMPNLNTSIL
                     AACPFLVPPLKEQHAIAHILGTLDDKIELNRRMNETLEAMA"
     gene            complement(617..1234)
                     /locus_tag="AC16BC_09520"
     CDS             complement(617..1234)
                     /locus_tag="AC16BC_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HIR3241899.1"
                     /translation="MGAKPTASRSTAVEPIEPLIQEIRGEKVIVDVDLANIYGVETRV
                     LNQAVRRNVEKFPSDFLFRLTPAEVRRVNRSQTVIGPQKHRNPRYRPFAFTEHGAIMA
                     ANILNSPRAVQMSVYVVRAFIAMRRTLGDTRDQARKLSALEKELKERLDVHEAAIVTI
                     LQRVMDIIDPPAPPESPGPPCKEIGFKAREGRPRYGRYAAAQNDT"
     gene            complement(1224..>1629)
                     /locus_tag="AC16BC_09525"
     CDS             complement(1224..>1629)
                     /locus_tag="AC16BC_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HIR3241900.1"
                     /translation="RNKKNGRFRDRRGETLFIDARKLGTMADRVHRELTDADIAKIAG
                     TYHAWRGDKDAGEYADVPGFCKRAVLEDIRKHGHVLTPGRYVGAEAQEDDGEPFEEKM
                     KRLTATLEEQFAKSAELEKIIRANLKGLGYGR"
BASE COUNT          280 a          518 c          470 g          361 t
ORIGIN      
        1 gtgccatcgc ctccagtgtc tcgttcatac gccggttcaa ctcgatcttg tcgtccagcg
       61 tgccgagaat gtgggcgatg gcgtgttgtt cctttagcgg cggaacaaga aaagggcacg
      121 ccgccaagat tgaagtattt agattcggca tggtcgcacc gtgagcatgc cgaacgatcc
      181 attcacgcac gctagggtgt cctagataga aagctgcgta tctcggatcg acaccactct
      241 cccctaaccg aactcgaaga caaccagtac cacagagcca cccgtcttcc ttttcccgga
      301 ccaaagcacg ccgttcgacg tcgccacgac ggctatagac gatatcgcct ttgcggacca
      361 gataccgact cagccgctct gcgtcgtcgg gtgttatacg agcaatgcca tcttcgagga
      421 ttctgttgtc cccaatgttc tggggcatga tcgaaggtat tccttcggca acatagtccg
      481 ccgcatgcag ttggctacca aacggccccg tttgaatatc acccccgcca cgttcacatg
      541 caataccaag cgtggtgtac tcccaccttt taggagcagg accgaacaac tcctccctaa
      601 ccatgccgga gctccgtcat gtatcgttct gcgccgccgc gtaccgccca tatcgaggcc
      661 ttccttctcg ggctttaaat ccgatctcct tgcacggggg ccctggggac tccggcggcg
      721 cgggcgggtc gatgatgtcc atgacgcgct ggaggatggt cacgatcgcg gcttcgtgga
      781 cgtccaggcg ctctttgagt tccttctcca acgcgctgag tttgcgcgct tggtcgcgcg
      841 tgtcgccgag ggttcggcgc atggcgatga acgctcgcac gacatacacg ctcatctgca
      901 cggcccgcgg gctgttgagg atgttggcgg ccatgatggc cccgtgctcg gtgaaggcaa
      961 aaggccgata gcgcgggttg cggtgcttct gcggaccgat cacagtttgt gatcggttga
     1021 cgcgcctcac ttccgccggg gtcaggcgaa agaggaaatc ggacgggaac ttctccacat
     1081 tgcggcggac cgcttgattg agcacccgcg tttccacgcc gtagatgttc gccaagtcga
     1141 cgtctacgat cactttttcg ccgcgaatct cctggatcag cggctcgatc ggttccaccg
     1201 cggtcgagcg cgacgctgtc ggcttagcgc ccatatccca gtcctttcag attggctcga
     1261 atgatttttt ccaattcggc tgatttcgca aactgctcct ccagcgtcgc ggtaagccgc
     1321 ttcatcttct cctcgaacgg ctcgccgtcg tcctcctgcg cctcggcccc gacatagcgt
     1381 cccggcgtga gcacgtggcc gtgcttgcgg atgtcctcga gcaccgcccg cttgcagaag
     1441 ccgggcacgt ctgcgtactc gccagcatcc ttgtcgcccc tccaggcgtg gtaggtgccg
     1501 gcgatcttcg cgatgtcggc gtcggtcagt tcgcgatgca cgcggtcggc catcgtgccg
     1561 agcttgcggg cgtcaatgaa gagcgtctcg ccgcggcggt cccggaagcg gccgttcttc
     1621 ttgttgcgg
//
LOCUS       DBNGTT010000424         3151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_612551, whole genome shotgun sequence.
ACCESSION   DBNGTT010000424 DBNGTT010000000
VERSION     DBNGTT010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_612551"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="AC16BC_09530"
     CDS             <1..572
                     /locus_tag="AC16BC_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241901.1"
                     /translation="AASKKRSATKAKKATKPKKTRSTAAKRRTTKKTASRTAGKKTTA
                     ARKPAKKRPARKPARKTARAAKPKPKAKPAPKRRAAIKKKPVTRRAARAPKVAPVVVP
                     VVPANELKVGVVTHYYSHLSVAVVSLTDRGLRVGDRIHVKGHTSDFYQNVESLQVEHE
                     SVMAAQTGQAVGMKVSEHAREHDAVYLVT"
     gene            complement(596..1249)
                     /locus_tag="AC16BC_09535"
     CDS             complement(596..1249)
                     /locus_tag="AC16BC_09535"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HIR3241902.1"
                     /translation="MDEVKSMLDKMVAQRIPVDLLFDGQGQSCTGRLTGTRRTQDAEG
                     FTLEVVSGDAPRTASVVRGRAVVERVLYEFAAVVTRRLSDPLPVFVIRQPTEIHRIER
                     RVHHRVEPAPHVKLRVSVSVSGEWLPVDVHNLSEGGISFSSPRLSAFGEGHAVARLEL
                     TFDEHPPIVTSGVVRNVYSIRYPREVGPVFGVQWGRLTPDESARLAAHVAACRGLTK"
     gene            complement(1306..2427)
                     /locus_tag="AC16BC_09540"
     CDS             complement(1306..2427)
                     /locus_tag="AC16BC_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012731.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3241903.1"
                     /translation="MTLLGSMTPIDLLDGCTPETRRLLHVVHELVDEPHERIDAKRVV
                     TLFCTRELGLDPEDVEAACEEVAAHVRHEYLPGTVGHVYRALLRLTQPWRVRYPLLEA
                     AGMIGDHHDDDPGGPDLVSVRASEVSNVVLPLNRPPLLPLGLLNGRLDGAPIVPSHNL
                     SELWTAMEHIRQDPDESLDDLMAVMPGPDFPTGGVIWGVEAIRQLYESGSATLVLRAA
                     IDDELQGARTRVAVASLPPGVLIKRVVEQIREAARRGRVALTQMEDRSTPERVRIVLD
                     APGSVTTADLKAVLWADTDCERRVPCHLSFGAESGAVARPLREWLALANQRCTPAWRP
                     KRGPILDRVPTLREIMDRGGYESPLFDVIDQRRTKILTG"
     gene            complement(2473..2676)
                     /locus_tag="AC16BC_09545"
     CDS             complement(2473..2676)
                     /locus_tag="AC16BC_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3241904.1"
                     /translation="MRAFIKVPDITDEQAASLTRTLKAVEGVVDAEVFAEDHVASVLY
                     DVRADVDTLVAAIQRAGYDAHPL"
     gene            2774..>3151
                     /locus_tag="AC16BC_09550"
     CDS             2774..>3151
                     /locus_tag="AC16BC_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR3241905.1"
                     /translation="MWVRLQREITECTKCPRLVAYRERVAADKRRQYRDETYWGRPLP
                     AFGDRSARLVVIGLAPAAHGGNRTGRMFTGDSSGQWLARALHESGFANQPTSERRDDG
                     LTLHDALITAAVRCAPPDNKPTPA"
BASE COUNT          558 a         1121 c         1035 g          437 t
ORIGIN      
        1 cagcggcttc gaagaaacga tcggccacca aggcgaagaa ggcgaccaaa cccaagaaga
       61 cccgatcaac cgcggccaag cgacggacca cgaagaagac ggcctcgcgc acggccggca
      121 agaagaccac cgcggcgcga aaaccggcca agaaaagacc cgcgcggaag ccggccagga
      181 agaccgcacg cgccgccaag cccaaaccca aagcgaagcc ggctcccaag cgccgggccg
      241 cgatcaaaaa gaagcccgtc acgaggcgcg cggcccgggc gccgaaggtc gcgccggtcg
      301 ttgtgccggt ggtccccgcc aatgagctca aagtgggagt ggtcacgcat tattactcgc
      361 acctgtccgt ggcggtcgtg tcgctgacgg accgggggct gcgcgtcggc gaccggattc
      421 acgtcaaggg gcacacgagc gacttttatc agaacgtgga gtcgttgcag gtcgagcacg
      481 aatccgtcat ggccgcgcag accggccagg ccgtgggcat gaaggtgtcg gagcacgcgc
      541 gcgagcacga cgcggtctac ctggtgacgt aaacgcgtcg gccccggggg cgtcgctatt
      601 tcgtcaggcc gcggcacgcg gccacgtgcg ccgcgaggcg cgcagactcg tccggggtca
      661 agcgccccca ctgcaccccg aacacgggtc ccacttctct cgggtaacga atcgaataca
      721 cgttgcgcac cacgcccgac gtgacgatcg gcgggtgttc gtcgaaggtg agctccaacc
      781 gcgccacggc gtgcccctcg ccgaacgcgg agaggcgagg cgacgaaaac gagattccgc
      841 cctcgctgag attgtgcacg tccaccggga gccactcgcc ggacaccgac acggacaccc
      901 gcagcttcac gtggggagcc ggctccaccc gatggtggac ccggcgctcg atccggtgga
      961 tctcggtcgg ctgccggatc acgaagaccg gaagcgggtc cgaaagccgg cgcgtgacca
     1021 ccgcggcgaa ttcatacagc acccgctcga ccacggcccg tccccgcacc accgacgccg
     1081 tgcgcggggc gtcgcccgac accacctcga gcgtgaaccc ctcggcgtcc tgcgtgcgcc
     1141 gcgtcccggt caatcgcccg gtacacgact ggccctgccc gtcgaacaag agatcgaccg
     1201 ggattcgctg cgccaccatc ttgtccagca tcgatttcac ctcatccatc ccaggctcct
     1261 ttcaccgaga ggaaccgcac gcgttgtgac gccggttacg cccggtcatc cagtcagaat
     1321 cttggttcgc cgctgatcaa tgacgtcgaa caagggactc tcgtacccgc ctcggtccat
     1381 gatctcgcgc aacgtcggga cccgatcgag gatggggccg cgtttcggtc gccacgcggg
     1441 cgtgcaccgt tggttggcga gcgccagcca ctcgcgcagc ggacgcgcga cggctccgct
     1501 ctccgcgccg aacgacaggt gacacggcac gcgccgctcg cagtcggtgt cggcccacag
     1561 gacggccttg aggtcagcgg tcgtcaccga gcccggcgcg tccaagacga tccgcacgcg
     1621 ttccggggtc gagcggtctt ccatctgcgt gagcgccacg cgaccccgcc tggccgcctc
     1681 ccggatctgt tcgacgaccc gcttgatcaa cacgcccggc ggaagcgacg cgaccgccac
     1741 gcgcgtgcgc gcgccctgca gctcgtcgtc gatcgcggcc cgcagcacca gcgtggccga
     1801 gccgctctcg tacaattgcc ggattgcctc gacgccccag atcactccgc ccgtggggaa
     1861 atccggcccc ggcatgaccg ccatcaaatc atcgagcgac tcatccgggt cctgccgaat
     1921 atgctccatc gcggtccaga gctcggacag gttgtgggac gggacgatgg gcgcgccgtc
     1981 gaggcggccg ttgagaagcc ccaagggcaa caacggcgga cgattcaacg gcaacaccac
     2041 gttggacacc tcggacgcgc gcacgctgac caggtcgggc ccgcccggat cgtcgtcgtg
     2101 atgatccccg atcatgcccg cggcttccag gagcgggtac cggacgcgcc agggctgcgt
     2161 caggcggagg agcgcgcggt agacgtgccc gacggtgccg ggcagatact cgtgccgcac
     2221 gtgcgccgcg acctcttcac aagcggcttc cacgtcctcg ggatcaagcc ccagctcgcg
     2281 cgtgcagaac agcgtgacca cgcgtttggc gtcgatccgc tcgtgcggct cgtccaccag
     2341 ttcgtggacc acgtgcagga ggcggcgggt ttccggggtg cacccgtcca gcaggtcgat
     2401 cggagtcatc gagccgagga gcgtcatctc gcgatcacgt cccgcgacac ggcgggcggc
     2461 tttgccgtcg cttcagagcg ggtgggcatc gtatccggcg cgctgaatcg cggcgaccag
     2521 cgtgtcgacg tccgcgcgga cgtcgtacag caccgaggcc acatgatcct cggcgaacac
     2581 ctcggcgtcc acgaccccct ccacggcctt gagcgtccgc gtgagcgacg cggcctgttc
     2641 gtcggtgatg tccgggactt tgatgaacgc tctcacattg attcccgcgc ggcgtgaagc
     2701 gtaactatag taaatccccc agcgtcgcgc aagactggac ggccgttccg gcggcgcgct
     2761 acaatccgcc gccatgtggg tgcgactcca acgcgagatc accgagtgca ccaagtgtcc
     2821 ccggttggtc gcgtaccggg aacgcgtggc cgcggacaag cgccgtcagt accgcgacga
     2881 gacgtactgg gggcggccgc tgcccgcgtt cggcgatcgg agcgcccgac tggtcgtcat
     2941 cgggttggcg ccggccgcgc acggcggcaa tcgcaccggc cgcatgttca cgggtgattc
     3001 gagcggccag tggttggccc gcgcgctgca cgagtcggga ttcgccaatc aaccgacgtc
     3061 ggagcgccgc gacgacggat tgacgctcca cgacgcgctg atcacggccg cggtgcgctg
     3121 cgcgccgccc gacaacaaac ccacgccagc g
//
LOCUS       DBNGTT010000425         4497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3422556, whole genome shotgun sequence.
ACCESSION   DBNGTT010000425 DBNGTT010000000
VERSION     DBNGTT010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3422556"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC16BC_09555"
     CDS             complement(<1..570)
                     /locus_tag="AC16BC_09555"
                     /inference="COORDINATES: protein motif:HMM:NF025871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIR3241906.1"
                     /translation="MAAQLTFQEQLLRVMDRKDHWAWKSFSSGAVTIPQLKIHFQQEY
                     EVYVRDFPVFLARVHGKNPPMPVRRALAENLYEEETGGLTRGRAHPEMFLYLMEGLKF
                     TAKDFDGIHLLPESAAYRRWLDQATTTGSWIEGAAVATIWVEGSVHERAVFDDTVREE
                     PDIERKIAEHPLVKYHGVPPRHLELARAHG"
     gene            687..1025
                     /locus_tag="AC16BC_09560"
     CDS             687..1025
                     /locus_tag="AC16BC_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HIR3241907.1"
                     /translation="MKIGILLTSGADHEDGYLVGKLSEAWLKLGHEVELFLMGEGVYH
                     AQSFSSPKRPYAALPGVLGLGAKVSICGVTAEARGISEADLYPGVEIASQYELSRIVD
                     RADRFLTFGY"
     gene            1032..1376
                     /locus_tag="AC16BC_09565"
     CDS             1032..1376
                     /locus_tag="AC16BC_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3241908.1"
                     /translation="MKHVVVAVRSHPLTTGRVHEALRMALGMTLSNHRVTVAYTGDGA
                     CAALKLDGERLQRPGLAESLELLDACHIREVVESDALTARHRGALRSRVEPVSRADVV
                     ALLKTSDVVIPW"
     gene            1370..1633
                     /locus_tag="AC16BC_09570"
     CDS             1370..1633
                     /locus_tag="AC16BC_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241909.1"
                     /translation="MVAGRALHVVRRRDDDMAWAVIAEQNRESPVDVLLVQDGVFSEP
                     PAGVSVCVNDEDVAARGVDTPHRRVGYEEIAGMVVNAATVKVW"
     gene            1688..1930
                     /locus_tag="AC16BC_09575"
     CDS             1688..1930
                     /locus_tag="AC16BC_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR3241910.1"
                     /translation="MAGTYTVAQVLDCKGLNCPLPVIKTKRAIEQLSVGQILQMIATD
                     PGSKPDMAAWSKSTGHELVETREESGVFTFFIRKAK"
     gene            1975..2571
                     /locus_tag="AC16BC_09580"
     CDS             1975..2571
                     /locus_tag="AC16BC_09580"
                     /inference="COORDINATES: protein motif:HMM:NF025066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR3241911.1"
                     /translation="MGLTSQEVDQVVAAKVKELAEHRLEELVREQVKAALEAEARKTK
                     PKRVAIVASKGTLDAAYPPLILATTAAALDMEAGIFFTFFGLQIVKKSGLENLKFVPV
                     ANPAMPMPMPNLLGALPGMTGLATLMMKQTIKQKHIASIPELMGLAKECGVKLWPCQM
                     TMGMMDIKREELIDGLQEPVGAATFLEFASNSDITLFV"
     gene            2653..2835
                     /locus_tag="AC16BC_09585"
     CDS             2653..2835
                     /locus_tag="AC16BC_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241912.1"
                     /translation="MSEMLVVSSKIKRLVREKAGFNTSAETLDALSQRVEKLCLDAID
                     RARADGRKTVKARDIV"
     gene            3162..4421
                     /gene="rho"
                     /locus_tag="AC16BC_09590"
     CDS             3162..4421
                     /gene="rho"
                     /locus_tag="AC16BC_09590"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3241913.1"
                     /translation="MNLSELKEKTIADLNEVAKDLKIEGASNLRKQELIFAILQAQAE
                     KNGTIYGEGVLETLQDGFGFLRSPDYNYLPGPDDIYISPSQIRRFNLRTGDVVSGQVR
                     PPKEGERYFAMLKVEKINFEDPEVARDKILFDNLTPLYPMEKIRLEAPGGDDISTRVM
                     ELVAPIGKGQRGLIVASPRTGKTVLLQNIAKAIMKNHPEITLIVLLIDERPEEVTDWQ
                     RQVKAEVVSSTFDEPPQRHAQVAEMVLEKAKRLVEHRHDVVILLDSVTRLARAYNTIA
                     PPSGKVLSGGLDANALQRPKRFFGAARNIENGGSLTIMATALIDTGSRMDDVIFEEFK
                     GTGNMEIHLDRKLSDRRVFPSIDIQKSGTRKEELLIPKEDLNRVWVLRKVLNPLSPVE
                     SMELIIDKLSKTKANADFLSSMSNGGR"
BASE COUNT          900 a         1306 c         1477 g          814 t
ORIGIN      
        1 gccgtgcgcg cgggccaatt ccaggtggcg gggcgggacg ccgtggtatt tgaccagcgg
       61 atgttccgcg atcttgcgct cgatgtcggg ttcttcacgg accgtgtcgt cgaagaccgc
      121 gcgctcatgc acgctgcctt ccacccagat cgtggccacg gcggccccct cgatccacga
      181 tccggtcgtg gtggcctggt cgagccatcg ccgatacgcc gcgctctccg gcagcaagtg
      241 gatcccgtcg aagtctttcg cggtaaattt cagcccttcc atgagataga ggaacatctc
      301 cgggtgggcg cggcctcgtg tgaggccgcc ggtttcctct tcatagaggt tttccgccag
      361 cgcgcgccga accggcatcg gaggattctt gccgtggacc cgcgccagga acacggggaa
      421 gtcgcgaacg tagacctcgt attcctgctg gaagtgaatc ttgagttggg ggatggtgac
      481 ggctccgctc gagaaggatt tccacgccca gtggtccttc cggtccatca cgcgtaagag
      541 ttgttcttga aacgtcagct gcgctgccat gcggatccct ccgttcgtcg gtcgcgtcgt
      601 gcgcgggcgc attgtatcac aggatactat tgatagcgcc gcgggattcg gttgacaaac
      661 gttttcgggc catcatacaa ttcgccatga aaatcggcat cttgctcacc agcggcgccg
      721 atcacgagga cggatacctc gtggggaagc tttccgaggc gtggttgaag ctggggcacg
      781 aggtcgaatt gttcctcatg ggtgaaggcg tctatcacgc gcaatcgttc tcgtcgccga
      841 agcgaccgta cgccgcgctc cccggcgtgc tgggtcttgg cgcgaaggtg tccatctgcg
      901 gcgtcacggc ggaagcccga gggatctccg aagccgacct gtatccgggc gttgaaatcg
      961 ccagtcaata cgaactgtcc cgaatcgttg atcgcgccga ccggttcttg accttcgggt
     1021 attgatcggc ggtgaaacac gtggttgtcg cggtccgaag ccacccgctc acgacgggac
     1081 gcgtgcacga agcgcttcgg atggcgctgg gcatgacgct gtcgaatcat cgcgtgaccg
     1141 tcgcgtatac cggcgacggc gcgtgcgcgg cgttgaagtt ggacggagag cgcctccagc
     1201 gtcccggcct tgccgagtcg ctcgaactgt tggacgcttg tcatattcga gaagtggtcg
     1261 agtctgacgc gttgacggcc cgtcaccgag gtgccctccg ctcccgcgtg gaacccgtga
     1321 gccgcgcgga cgtggtcgcg cttctgaaga cgtccgacgt ggtgatcccg tggtagcggg
     1381 tcgggcgctg cacgtggtgc gccggcggga cgacgacatg gcgtgggccg tgatcgccga
     1441 gcagaaccgg gagtcgccgg tggacgtgtt gttggtgcag gacggcgtgt tctcggagcc
     1501 accggccggc gtgtcggtgt gtgtcaatga tgaagacgtc gcggcccgcg gagtcgacac
     1561 gccgcaccgc cgggtggggt acgaggagat cgccggcatg gtcgtgaacg ccgcgaccgt
     1621 gaaggtgtgg tagcgccggg ccggatcgtg gatttggccg gcggctgact gggaaagggt
     1681 ggaaacgatg gcgggaactt acacggtagc ccaagtcctc gactgcaagg ggctcaattg
     1741 tccgctgccg gtgatcaaga ccaagagggc gatcgagcaa ctgtcggtcg ggcaaatcct
     1801 tcagatgatc gcgaccgacc ccggctccaa gcctgatatg gcggcttggt ccaagagcac
     1861 ggggcacgag ttggtggaaa cccgggagga atcgggcgtg ttcacgtttt tcattcggaa
     1921 ggccaagtag cggacacggc cgccgcgccg cgtcccggtg gaatgagggc acccatggga
     1981 ctgacatcgc aggaagtcga tcaggttgtc gccgccaagg tcaaggagtt ggcggagcac
     2041 cgactggaag aactcgtccg cgaacaggtg aaggcggcgc tcgaggccga agcgcgcaag
     2101 accaagccga aacgcgtggc gatcgtggcg tccaagggca ccctggacgc cgcgtatccc
     2161 cccttgatcc tggcgacgac cgcggcggct ctggacatgg aggcgggcat cttcttcacg
     2221 ttcttcggat tacagatcgt caagaagagc ggcctggaga atctcaagtt cgtgccggtg
     2281 gcgaatccgg ccatgccgat gcccatgccc aatttgctcg gcgcgctgcc cggcatgacg
     2341 gggctcgcca ccctgatgat gaagcagacg atcaagcaga aacatatcgc ctcgattccg
     2401 gaactcatgg ggctggccaa ggagtgcggc gtgaagctct ggccctgcca gatgacgatg
     2461 ggcatgatgg acatcaaacg cgaggagttg atcgacggtc tgcaagaacc ggtgggcgcg
     2521 gcgacgtttt tggagtttgc gtcaaacagc gacatcacgc tgtttgtgta acgagacggc
     2581 tcccctcacg gggggatggc ggtgcgccgg cgcctcgccg gcacgcgaag gacaactgag
     2641 aggaggaacg gtatgtcgga gatgttggtg gtgtcgtcga agatcaaacg attggtccgc
     2701 gagaaggcgg ggttcaatac gtcggcggaa accctcgatg cgctgagtca gcgggtcgaa
     2761 aagctgtgtc tcgatgccat cgaccgcgcg cgggccgacg gacgcaagac cgtgaaggcg
     2821 cgcgacattg tctgagccga cgagtcgccg cggttcggtg tcggcgccct gacgtcgggt
     2881 accggggcga cacttcccaa ttgacactta tgacgcggct tcgtataatg gtaatagttt
     2941 ccgtttcttc caaaccctga ttcctgaaaa ccaattcgcg gcgacacaga gatccaatcc
     3001 ccaaaggcgt gttgaagacc gacggatttt gcagtaggac agattaaacc atcaatcgga
     3061 ccaaccactc tccgacgcac gatgcgtgat tgtttgaacg cgatgttgga ccatcgcggt
     3121 atccgcacgt gcggcgcgac cgcgggcctg caggaggacc catgaatctg agcgagctaa
     3181 aagaaaaaac gatcgccgac ctcaacgaag tcgccaagga tttgaagatc gaaggcgcga
     3241 gcaacctgcg caagcaggaa ttgatcttcg ccattcttca agcccaggcg gaaaaaaacg
     3301 ggaccattta cggcgaaggg gtcctcgaga cgcttcagga cgggttcggc ttcctccgct
     3361 cgcccgacta caactacctg ccgggaccgg acgacatcta tatttcgccg tcgcagatcc
     3421 gccggttcaa cctgcggacc ggcgacgtgg tgtcgggtca ggtccgaccg cccaaagagg
     3481 gtgagcgcta cttcgcgatg ctcaaagtgg aaaagatcaa ctttgaggat ccggaggtgg
     3541 cgcgcgacaa gatcctcttc gataatctga ccccgctgta tcccatggag aaaatccggc
     3601 tcgaagcgcc gggcggcgac gatatctcca cgcgggtcat ggagttggtg gccccgatcg
     3661 gcaagggaca gcgcggactg atcgtggcct ctccccggac ggggaagacc gttctgcttc
     3721 agaacatcgc caaagcgatc atgaagaacc atccggagat cacgctgatc gtgctcctca
     3781 tcgatgaacg gcccgaagag gtgaccgatt ggcaacgtca ggtcaaggcc gaggtggtga
     3841 gttccacgtt cgacgagccc ccgcagcgtc acgcgcaggt ggccgagatg gtgctggaga
     3901 aagccaagcg tctggtcgag caccggcacg acgtggtgat cctgctcgac agcgtgacgc
     3961 gtctggcgcg cgcctacaac acgatcgcgc cgccgagcgg caaggtgtta tccggcgggc
     4021 tcgacgccaa cgccctgcaa cggcccaaac ggttcttcgg cgcggcgcgc aacatcgaaa
     4081 acggcggcag tctgaccatc atggcgacgg ccctgatcga caccggcagc cggatggacg
     4141 acgtgatctt cgaagagttc aagggcaccg gcaacatgga gatccatctg gatcgcaagc
     4201 tgtcggaccg ccgcgtgttc ccgtcgatcg acatccagaa gagcggaacg cgtaaagagg
     4261 aattactgat tccgaaagag gatctcaatc gcgtctgggt gctgcgcaag gtgctgaacc
     4321 cgctctcgcc cgtcgagtca atggaactca tcattgacaa gctctccaag accaaggcca
     4381 acgcggactt cttgtcatcg atgtccaacg gcgggcggta ggcctcggac cgagccaaca
     4441 accatagcgg attgccgttc aagcgattca atgccccgca ggggtaggag aatcagc
//
LOCUS       DBNGTT010000426         9416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2656695, whole genome shotgun sequence.
ACCESSION   DBNGTT010000426 DBNGTT010000000
VERSION     DBNGTT010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2656695"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="cysS"
                     /locus_tag="AC16BC_09595"
     CDS             complement(<1..880)
                     /gene="cysS"
                     /locus_tag="AC16BC_09595"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3241914.1"
                     /translation="MSRHASGDLTLRLYNTLSGTKEVLRTRVPRHLGMYVCGVTVYDL
                     CHIGHARSAIVFDVLRRYLESRGVIVTYVRNFTDIDDKIIHRAQRDGIGWREVADRYI
                     TEFYRDMDALGVGRATVEPKATDHIGDMLALIQGLIAKGKAYAEGSDVYYRVASFPGY
                     GKLSRRRLDQLRVGARVEVDERKHDPLDFALWKSSKPGEPAWDSPWGPGRPGWHIECS
                     AMAMAHLGQTIDLHGGGQDLTFPHHENEIAQSEGFTGVEFARCWVHNGFVTINEEKMS
                     KSLGNFFTIREILDKSP"
     gene            complement(877..1563)
                     /locus_tag="AC16BC_09600"
     CDS             complement(877..1563)
                     /locus_tag="AC16BC_09600"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3241915.1"
                     /translation="MDGDRVRPRLLAEQLAARGITDRRVLDAVAAVPRHLFVESALAD
                     RAYEDRALPIGERQTISQPYMVALMTQALELTGAERVLEIGTGSGYQAAVLAQLATRI
                     CSVERVKALADRARHTLDRLKLYHVVIKVFDGTFGWPGEAPFDAVIVTAGAPAVPKPL
                     IDQLKDNGRLVAPIGDRDGQRLVKVVKRKSGVQARVLTECAFVPLIGAHAWPADASAT
                     PLAVLGDSRR"
     gene            complement(1553..2329)
                     /gene="surE"
                     /locus_tag="AC16BC_09605"
     CDS             complement(1553..2329)
                     /gene="surE"
                     /locus_tag="AC16BC_09605"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3241916.1"
                     /translation="MSPRPKILISNDDGVHSAGLKRLAEALKAVGEVIVVAPDGERNA
                     ASHAITLHKPLRVTQLAEGVYSTSGTPTDCINLGVITILKERPALVVSGINLGENLAD
                     DVTYSGTVSAAMEGTLLGIPSIAMSQSGEGVFRFDAAAQVAVRLAEAVLEEGLPRDTL
                     LNVNVPDLDPADVRGVKITRLGRRSYDANDIIQNVDPRGRKYYWIGGNRLFGVDQEGT
                     DFGAVQAGYISVSPVHLDLTNYAALATLATWEPRLSTRGR"
     gene            complement(2626..2699)
                     /locus_tag="AC16BC_09610"
     tRNA            complement(2626..2699)
                     /locus_tag="AC16BC_09610"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2663..2665),aa:Pro,seq:ggg)
     gene            complement(2720..3160)
                     /locus_tag="AC16BC_09615"
     CDS             complement(2720..3160)
                     /locus_tag="AC16BC_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3241917.1"
                     /translation="MSASVRGSGQSSPRASRSAPVAPDKLFFKIGEVSRLTGLEAYVL
                     RYWETEFPALRPHKTAGGQRVYTRKDLDLVLQIKQMLYEQGFTIAGARKRLRSRSPEA
                     LIERRALLGEIRGELQSILNDLKACAPLRVSAIPGAKHEERRRS"
     gene            complement(3157..3504)
                     /locus_tag="AC16BC_09620"
     CDS             complement(3157..3504)
                     /locus_tag="AC16BC_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR3241918.1"
                     /translation="MRKADIATEVYEKLGIPKKDASDILELMLDSIKAVLQKGETVKI
                     AGFGNFVVRHKRARKGRNPKTGEEIGITPRKVVTFRPSQVFKHYVNDSQANEPSVVPP
                     ETAEAEEEEVDAP"
     gene            complement(3648..5240)
                     /gene="cimA"
                     /locus_tag="AC16BC_09625"
     CDS             complement(3648..5240)
                     /gene="cimA"
                     /locus_tag="AC16BC_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR3241919.1"
                     /translation="MKKKNEPTIELYDTTLRDGAQAEDIAFSVEDKLLIVQRLDELGV
                     PYIEGGWPGANPKDVEFFKEVRRLTLKQAQIAAFGATRRAKYRVQDDPSLKALLDAQT
                     PIITIFGKSWDLHVTEALGVSLKKNLELIGDSVGYLKSKRKMVVYDAEHFFDGYKANP
                     EYALATLARAQAAGSDRIALCDTNGGNMPWEVRDIVAAVAARVTVPLGIHAHNDAEVA
                     VANSLVAVEQGVVQVQGTINGFGERCGNANLCSLIPNLQLKLQRPVISTAQLAHLTDA
                     SRYVFEIANLPAHKRQPYVGESAFAHKGGVHVHAVRKKAETYEHVRPEVVGNRRRILV
                     SDAAGRGSLLEKAAEYGIDLDAGDPRLHDLLNTLKELERQGYQFEGADASFELLMRKA
                     LGTHRRFFDLIGFRVTVDKRREGEDPLCEATIMLRVGEDIEHTAAVGAGPVNALDHAL
                     RKALEGFYPQLRRVRLLDYKVRVLAAQGGTASRVRVLIESGDQERKWGTVGVSSNIIE
                     ASWQALVDGIEYRLLRGGSSRP"
     gene            complement(5334..6560)
                     /locus_tag="AC16BC_09630"
     CDS             complement(5334..6560)
                     /locus_tag="AC16BC_09630"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3241920.1"
                     /translation="MALIVQKYGGTSVGSPERIQAVAERVIRTKKAGDDVVVVLSAMS
                     GETDRLVGLAHKMSAAPDEREMDMLLSTGERVTIALLAMALRERGYPARSFTGRQVGI
                     VTDAVHTKARIERVTGERMREALAEGVIPVVAGFQGINEKSDVTTLGRGGSDTTAVAL
                     AAALKADRCDIYTDVDGVYTTDPNIVPNARRLDKISYEEMLEMASLGAKVLQTRSVEF
                     AMKYQVPVRVLSSFTDTPTGTLVTKEDREMEQVVVSGVAYDRNQAKITLLGVPDRPGI
                     AGRLFGGIAKENIVVDMIIQNISQDGLTDISFTVPKNEAKQAVALVKKLAQEIGARDV
                     ELSEGIAKVSIVGVGMRSHSGVAATMFETLAREGINIMMISTSEIKISCVMDAKYVEL
                     AVRALHEAFGLGEPRP"
     gene            complement(6645..7880)
                     /locus_tag="AC16BC_09635"
     CDS             complement(6645..7880)
                     /locus_tag="AC16BC_09635"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HIR3241921.1"
                     /translation="MKYLVLLADGMADLPIPRLQNRTPLDAAKTPNLDRLASDGELGL
                     VRTTPAGATPTSEITNLTIFGYDAKKYPQFGRSPLEAASLGVSVGKDDVVFRCNLVTL
                     KDEQRGYDIERLGPHVILEDPSAGHISSDDSRALIEELNSQLATEFLQFYAGVSYRHL
                     MVWIGGRARGSCTPPYDLAGKPVVGGLPTGDGSDMLRKVMESAVAVLTLHPLNVERVD
                     QGLKPANGVWLWGQGKAAELTPFAERFGKRGALIAAVDVMKGLALAVGFDVIHVPGAT
                     GYLDTDYQGKVDAAVAALNTHDLVVLHVEAPDEASHNGDLDAKLRAIEDFDAKVVGPV
                     IQATASEPALRVLALCDHVAPVSTRSHTPDPVPYLLHQRGGAAPAPSGARGFHEAAAK
                     ASGKLVADGHRLMEHFLKG"
     gene            complement(7881..8939)
                     /gene="thrC"
                     /locus_tag="AC16BC_09640"
     CDS             complement(7881..8939)
                     /gene="thrC"
                     /locus_tag="AC16BC_09640"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3241922.1"
                     /translation="MTGWPGIITHYRDLLPVTERTPIITLNEGNTPLIATKNLAAAIN
                     PDLRLFLKYEGANPTGSFKDRGMTLAISKAAEAGADAVICASTGNTSASAAAYGARAG
                     MRVFVLIPEGKIAMGKLAQAMIHRARVIQVEGNFDEALSIVKGVAERYPVTLVNSINP
                     FRLEGQKTGAFEVCDQLGRAPDVHVLPVGNAGNITAYWRGYKEYHARSRADRLPRMLG
                     FQAAGAAPIVLGHVVDKPRTIATAIRIGNPASWKSAVEAASESKGQIDLVTDDEIIEA
                     YRMIAGMEGVFCEPASAASVAGVIKLNKAGYFQGGETVVCTLTGHGLKDPDIAMSVSQ
                     KPTTIKARLEDVVKVLGY"
     gene            complement(8941..>9416)
                     /locus_tag="AC16BC_09645"
     CDS             complement(8941..>9416)
                     /locus_tag="AC16BC_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3241923.1"
                     /translation="VFNAIYVAGDFVGQTLFYGRGAGDQPTASAVVSDLVAIARNIRH
                     QAAGRVPPTSYDGASRRPLRLLAMEEISSLYYLRCSVLDNPGVLSTISGLLGKYNISI
                     SSVIQKGRKAGGAVSVVMMTHQALERDVRQALAEIDRLPSVVDKTVLIRVEGEGD"
BASE COUNT         1543 a         3162 c         2992 g         1719 t
ORIGIN      
        1 agggcgactt gtccaggatc tcgcggatcg taaagaagtt gcccagcgac ttggacatct
       61 tttcttcgtt gatcgtgacg aagccgttgt gcacccagca gcgcgcgaac tccacgccgg
      121 tgaagccctc ggactgcgcg atttcgtttt cgtggtgggg gaacgtcagg tcctgaccgc
      181 ccccgtgcag atcgatggtc tgccccaggt gcgccatggc catggccgaa cactcgatgt
      241 gccaccccgg acggcccggg ccccacgggc tgtcccacgc gggttcgccc ggtttggagg
      301 atttccagag cgcgaagtcc aggggatcgt gtttgcgttc gtccacctcc acgcgcgcgc
      361 cgacccgcaa ctgatcgagc cgccgccgcg agagcttgcc gtagcccggg aagctggcca
      421 cgcggtagta gacgtcgctt ccctccgcgt aggccttgcc cttcgcgatc aagccctgga
      481 tcaacgcgag catgtcgccg atgtggtcgg tggccttggg ctcgaccgtg gcgcggccca
      541 cgcccaatgc gtccatgtcc cggtagaact ccgtgatgta gcggtcggcc acctcgcgcc
      601 agccgatgcc gtcgcgttgc gcccgatgga tgatcttgtc gtcgatgtcg gtgaagttgc
      661 ggacataggt gacgatcacc ccgcgcgatt ccaggtagcg acgcaacacg tcgaacacga
      721 tcgcgctgcg cgcgtgtccg atatggcaga ggtcgtatac ggtcaccccg cacacgtaca
      781 tgcccaggtg gcgcggcacg cgcgtccgca acacctcctt ggtcccgctc aaggtgttgt
      841 acaggcgcag cgtgagatcc cccgacgcgt ggcggctcac cggcggctgt ccccgagcac
      901 ggcgagcggg gtggccgacg cgtccgcagg ccaggcgtgc gcgccgatga gcggcacgaa
      961 cgcgcactcg gtcaggaccc tggcttgcac gccggatttt cgcttgacca ccttgaccag
     1021 ccgttgtccg tcgcggtcgc cgatcggcgc gaccagccgg ccgttgtcct tgagttggtc
     1081 gatcagcggt ttgggcacgg cgggcgcgcc ggccgtgacg atcaccgcgt cgaacggggc
     1141 ttccccgggc cagccgaagg ttccgtcgaa caccttgatg accacgtggt acaacttgag
     1201 gcggtcgagg gtgtggcgcg cgcggtcggc cagcgctttg acgcgttcca ccgagcagat
     1261 cctggttgcg agctgggcca acaccgcggc ctgatacccg gacccggtgc cgatttccag
     1321 gacgcgttcc gcgccggtca actccagagc ctgcgtcatc agggcgacca tatagggctg
     1381 ggagatcgtc tggcgctcgc cgatgggcag cgcgcgatcc tcgtacgcgc gatccgcgag
     1441 ggccgactcg acaaagagat ggcggggcac ggccgccacc gcgtccaaca ctcggcggtc
     1501 cgtgatgccg cgcgccgcca actgctcggc caacaggcgg ggccttacgc gatcaccgtc
     1561 cacgggtcga aagtcgcggt tcccaggtcg ccaaggtggc gagcgccgcg tagttggtca
     1621 ggtcgaggtg aaccgggctc accgagatgt agccggcctg caccgcgccg aagtccgtcc
     1681 cctcctggtc gaccccgaac aggcggttgc cgccgatcca atagtacttg cggccgcgcg
     1741 ggtccacgtt ctggatgatg tcgttggcgt cgtacgatcg gcgtcccagc cgcgtgatct
     1801 tcacgccccg cacgtcggcg ggatccaggt ccggcacgtt gacgttgagc agggtgtccc
     1861 ggggcaggcc ttcctcgagc acggcctccg cgagccgaac cgccacctgc gcggccgcgt
     1921 cgaaccggaa cacgccttcg ccggattggg acatggcgat ggacgggatg cccagcaagg
     1981 tgccttccat ggccgcggac accgtgccgg agtacgtcac gtcatcggcc agattttcgc
     2041 ccaggttgat gccggagacg accaacgcgg gccgctcctt cagaatggtg atcacgccca
     2101 agttgatgca gtcggtcggg gtgccgctgg tgctgtatac gccctcggcg agttgcgtga
     2161 cccgcaacgg cttgtgcagg gtgatggcgt ggctggccgc attgcgctcg ccgtccggcg
     2221 ccaccacgat cacctcgccc acggccttga gcgcttccgc cagccgcttg agaccggcgg
     2281 agtggacgcc gtcatcattg gagatgagga ttttgggtcg gggggacatg cagatgctgc
     2341 aggcgttaca ggattggcct gcgactcaaa ctccgtcagc ggtagccgtt cgagagatca
     2401 cccttaaaga ggaaaggcac tacccgaggc gaaagatctc gcagcctcgg gccggggaac
     2461 ctgcactgcg ccatatggcg cagaatacaa ataggctgct tgccgtacag gtggcgaagc
     2521 aagtcaggcc ctacgttgcc cttcgcgggc gagtgattcc gaaaccttct ccgagcctcg
     2581 gcgcgggaca acgcgcctcc gttcggtttc gcaagcgagg atgggtcggg gcgactggat
     2641 ttgaaccagc gaccactcgc accccaagcg agtgcgctac caggctgcgc tacgccccga
     2701 atacccccac gatcccgtgc tacgatcgcc tccgctcctc gtgtttcgcg cctggaatcg
     2761 cggatacgcg aagcggagca caggccttca gatcgttcag aatcgactgc aactctcccc
     2821 ggatttcacc cagcagcgcg cgacgctcga tcaacgcttc cggggagcgg ctgcggagcc
     2881 gcttgcgcgc gccggcaatc gtgaacccct gctcgtacaa catctgcttg atctggagaa
     2941 ccagatccag atccttcctc gtatacacgc gttgtccccc cgcggtcttg tgggggcgca
     3001 gcgcgggaaa ttcggtttcc cagtaccgta gcacgtacgc ttcgagaccg gtgagtcgac
     3061 tgacctcgcc gatcttgaaa aacagcttgt cgggtgccac cggggccgac cgcgacgcgc
     3121 ggggagacga ctgccctgaa ccgcggaccg acgcgctcac ggcgcgtcca cctcttcctc
     3181 ttcagcctcg gcggtctccg ggggcactac cgacggctcg ttggcctgcg agtcgttgac
     3241 gtagtgcttg aagacctgac tggggcggaa cgtcaccacc ttgcgcggcg tgatgccgat
     3301 ttcctcgccg gtcttggggt tgcggccctt ccgcgcccgc ttgtggcgca ccacgaagtt
     3361 cccgaacccc gcgatcttga ccgtttcgcc cttctgcaac accgccttga tggaatcgag
     3421 cattaattcc aagatatccg aggcgtcctt tttgggaatc cccagcttct catacacttc
     3481 agtcgcgatg tcggcctttc gcatggcaca cactccgacc gtttacacgt caggcaagca
     3541 atggcgtcga ctgctacgac actctgccac cctaacgaaa caccggctca aagtcaagga
     3601 aaatcaactg gttactcggt gatcgcagaa tgcgccgagc gcgtcgatca ggggcgcgag
     3661 cttccgcctc gaagcaggcg gtactcgatg ccgtccacca acgcctgcca gctcgcctca
     3721 ataatattgg acgagacgcc cacggtgccc cacttgcgct cctgatcgcc ggattcgatc
     3781 aacacccgaa cgcgcgatgc ggtgccgccc tgcgctgcca gcacgcgaac cttgtagtcg
     3841 agcaggcgga cccgtcggag ctgtgggtag aacccctcca gggccttgcg cagggcgtgg
     3901 tccaaggcat tgaccggtcc cgctcccact gcggcggtgt gctcgatgtc ctcgcccacg
     3961 cgcagcatga tggtggcttc gcagagcggg tcctcgccct cgcgtcgctt gtcaacggtc
     4021 acgcggaacc cgatcaaatc gaaaaagcga cggtgcgtgc ccagcgcttt gcgcatgagc
     4081 aattcaaagg aggcgtccgc gccctcgaac tggtatccct ggcgctcgag ttctttcagt
     4141 gtgttcaaca aatcgtgcag acggggatcg ccggcgtcga gatcaatccc gtattccgcg
     4201 gccttctcga gcaggctgcc gcgtcccgcc gcgtcggaca ccagaatgcg gcggcgattg
     4261 ccgaccacct cgggccgcac gtgttcgtag gtttcggcct tcttgcgcac cgcgtgtacg
     4321 tgcacgccgc ccttgtgcgc gaacgcgctc tcgcccacgt acggctggcg cttgtgggcg
     4381 ggaaggttgg cgatttcgaa cacgtagcgc gacgcgtcgg tgaggtgcgc cagttgggcg
     4441 gtgctgatca ccgggcgctg caatttcagc tgcaaattcg gaatcagcga acacaggttc
     4501 gcgttgccgc agcgctcgcc gaacccgttg atcgtgcctt ggacctggac caccccttgc
     4561 tcgaccgcca cgagcgagtt ggccacggcc acttccgcgt cgttgtgcgc gtggatcccg
     4621 agcggcacgg tgacgcgcgc tgccacggcc gccacgatgt cgcgcacctc ccacggcatg
     4681 ttcccgccgt tggtgtcgca caacgcgatg cggtccgagc ccgcggcttg cgcgcgggcc
     4741 agcgtggcca gggcatactc ggggttggct ttgtacccgt cgaaaaagtg ctccgcgtcg
     4801 tagaccacca tcttccgttt ggatttcagg tagcccaccg aatcgccgat cagctccagg
     4861 ttcttcttga gcgagacgcc caaggcttcg gtcacgtgca aatcccagct cttgccgaaa
     4921 atcgtgatga tgggcgtctg cgcgtcgagc agggctttga gcgacgggtc gtcctgcacc
     4981 cgatatttgg cgcgtcgcgt ggcgccgaac gccgcgatct gcgcctgttt caatgtgagt
     5041 cggcggacct ctttgaagaa ctccacgtcc ttcggattcg cccccggcca cccaccctcg
     5101 atgtacggca cgcccagctc gtcgagccgc tgcacgatca gcagcttgtc ctcgaccgaa
     5161 aacgcaatat cctccgcctg cgccccatcc cggagggtcg tgtcgtagag ctcgatggtt
     5221 ggctcgttct tttttttcat gttttcccgc gaagcggaac cccgttcaga aacgggccag
     5281 atgcaaggcg ccgcgagcac cggcagcgca gcggcaacgc cgcagatggc cgtttatgga
     5341 cggggttcac caaggccgaa ggcctcgtgg agcgcgcgga cggccaactc cacatacttc
     5401 gcatccatca cgcacgaaat cttaatctcg ctggtcgaga tcatcatgat gttgatgcct
     5461 tcacgggcca acgtttcgaa catggtcgcg gccacgcccg agtgcgagcg catgcccacg
     5521 cccacgatcg acactttggc gatgccttcg gacagctcca cgtcgcgcgc gccgatctcc
     5581 tgcgcgagct tcttgaccag cgccacggcc tgtttcgcct cgttcttggg aaccgtgaag
     5641 gaaatgtcgg tcaggccgtc ctggctgatg ttctggatga tcatgtcgac gacgatgttt
     5701 tccttggcga tgccgccgaa caaacgcccc gcgatgccgg gacggtccgg cacgcccaat
     5761 agggtgatct tggcctgatt gcggtcgtag gcgacgcccg agaccaccac ctgttccatc
     5821 tcacgatcct ccttcgtcac gagagtgccg gtgggggtgt cggtgaagct ggacagcacc
     5881 cgcaccggaa cctggtactt cattgcgaat tccaccgacc gggtctgcaa caccttggcc
     5941 ccgaggctgg ccatctcgag catttcttcg tacgagatct tgtccagacg gcgcgcgttg
     6001 ggcacgatgt tggggtcggt ggtgtacacg ccgtccacgt cggtgtaaat gtcgcagcga
     6061 tcggctttga gcgcggcggc cagcgcgacc gcggtggtgt cggagccgcc gcggcccagc
     6121 gtggtcacgt cggacttttc attgatccct tgaaagcccg caaccaccgg gatcacgcct
     6181 tcggccaggg cttcgcgcat gcgctcgccg gtcacccgct cgatgcgggc cttggtgtgc
     6241 accgcgtcgg tcacgatgcc gacctggcgt ccggtgaacg agcgcgcggg atatccacgc
     6301 tcgcgcaagg ccatggccaa taacgcgatg gtgacccgtt cgcccgtgga cagaagcatg
     6361 tccatctcac gctcgtccgg cgcggcgctc attttatgcg cgagtccgac gagccggtcg
     6421 gtctcgccgc tcatggccga caacaccacc acgacgtcat cgccggcctt cttggtccgg
     6481 atcacgcgct cggccacggc ctggatgcgc tccgggctgc ccaccgaggt gccgccgtat
     6541 ttctggacga tcaatgccaa atgaacgatc ccatgagccg gtttaccggt tctccggttg
     6601 aagccccacg caccggtccc gtcaaccggt gaaccagcgc accactatcc cttcaggaaa
     6661 tgctccatga gccggtggcc gtcggccacg agtttaccgc tggccttggc cgcggcctcg
     6721 tgaaacccgc gtgctcccga aggcgccggg gccgcgccgc ctcgttggtg caggaggtac
     6781 gggaccgggt cgggcgtgtg cgaccgggtg gaaaccgggg ccacgtgatc gcacaaggcc
     6841 aacacgcgca atgcgggctc ggacgcggtg gcctggatca cgggtcccac gaccttggcg
     6901 tcgaaatctt cgatcgcgcg gagcttggcg tccaggtcgc cgttatggct ggcttcatcc
     6961 ggcgcttcca cgtggagcac gaccaagtca tgcgtgttga gcgccgcgac cgcggcgtcc
     7021 accttgccct ggtaatcggt gtccagatac ccggtggcgc ccggcacgtg gatcacgtcg
     7081 aatcccacgg ccagcgcgag ccccttcatc acatccacgg ccgcgatcag ggcgccgcgt
     7141 ttgccgaacc gctcggcaaa cggcgtcaac tccgcggcct tgccctggcc ccacagccag
     7201 acgccgttcg cgggcttgag accttggtcc acgcgttcca cgttgagcgg gtgcagggtc
     7261 aggaccgcga ccgcgctctc catgaccttg cgcagcatgt ccgagccgtc gccggtgggc
     7321 aatccgccga ccacgggttt cccggccaga tcgtagggcg gggtgcagct cccccgcgcg
     7381 cgaccgccga tccacaccat caggtgccgg taactcacgc ccgcgtagaa ctgcaggaac
     7441 tcggtggcca actggctgtt caactcctcg atcaacgcgc gcgaatcgtc cgagctgatg
     7501 tgccccgcgc tcgggtcctc caggatcacg tgcgggccga gccgttcgat gtcgtacccg
     7561 cgctgttcgt ccttgagcgt gaccaggttg caccggaaga ccacgtcgtc cttgcccacg
     7621 cttaccccga ggctggcggc ttcgagcggg gagcgtccga actgcgggta cttcttggcg
     7681 tcgtatccga agatggtgag gttggtgatc tcgctggtcg gcgtggcccc agccggcgtg
     7741 gtccgcacca gacccagctc cccgtcgctc gccaatcggt cgaggttcgg cgtcttggcc
     7801 gcgtccaacg gagtccgatt ctgcaaccgt ggaatcggca gatcagccat gccgtcggcg
     7861 agcaacacca ggtatttcat ctaatacccc aggaccttca cgacgtcttc caaccgcgct
     7921 ttgatggtgg tcggcttctg ggacacgctc atggcaatgt ccgggtcctt gagcccgtgg
     7981 ccggtgaggg tgcagaccac ggtttccccg ccttgaaaat aacccgcttt gttgagcttg
     8041 atgacccccg cgaccgacgc ggccgacgcg ggctcgcaaa agacgccttc catccccgcg
     8101 atcatgcgat acgcctcgat gatctcgtcg tcggtgacca ggtcgatctg ccccttggat
     8161 tcggacgcgg cttcgaccgc ggacttccaa ctcgcggggt tgccgatgcg gatcgcggta
     8221 gcgatggtgc ggggcttgtc caccacgtgg ccgagcacga tcggcgccgc gcccgcggcc
     8281 tgaaacccga gcatgcgcgg gaggcgatcc gcgcggcttc gcgcgtgata ttccttgtac
     8341 ccgcgccagt acgcggtgat gttgcccgcg ttgcccacgg gcaatacgtg cacgtcgggc
     8401 gcgcggccga gttgatcgca cacctcgaac gccccggtct tctggccctc cagtcggaaa
     8461 gggttgatgg agttcacgag cgtgacggga taccgctccg ccacgccctt gacgatggag
     8521 agggcctcgt cgaagttgcc ctcgacctgg atgacgcgcg cgcgatggat catggcctgc
     8581 gccagcttgc ccatcgcgat cttgccctcg gggatcaaca cgaacacgcg catcccggcg
     8641 cgcgcgccgt aggccgcggc cgacgccgac gtgttgccgg tggacgcgca gatcaccgcg
     8701 tccgcgcccg cttccgcggc cttggaaatc gcgagggtca tgccgcggtc cttgaacgag
     8761 cccgtggggt tcgcgccctc gtatttgagg aacagacgca aatcgggatt gatggcggcc
     8821 gcgaggttct tggtcgcgat gagcggggtg ttgccctcgt tgagcgtgat gatcggggtc
     8881 cgctcggtga ccgggagcag gtcgcgataa tgggtgatga tgccgggcca gccggtcacg
     8941 tcagtcgccc tccccctcga cgcggatcaa cacggtcttg tccaccaccg acggcagccg
     9001 gtcgatttcg gccaaggctt ggcgcacgtc gcgttcgagc gcctggtgcg tcatcatgac
     9061 caccgacacc gcgcccccgg ccttgcggcc tttttgaatc accgacgaaa tgctgatgtt
     9121 gtatttgccg agcaatccgg agatcgtgga cagcacgccg ggattgtcca acaccgagca
     9181 gcgcaggtag tacagactcg agatctcttc catggccagc agccgcagcg gccggcggct
     9241 cgccccgtcg taggacgtgg gggggacgcg gcccgcggcc tggtggcgga tgttgcgcgc
     9301 gatggcgacc aagtcgctga ccaccgcgct cgcggtgggt tgatcgcccg cgccgcggcc
     9361 gtaaaacaac gtctgtccga cgaagtcgcc cgccacgtag atggcgttga acacgc
//
LOCUS       DBNGTT010000427         2799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_613107, whole genome shotgun sequence.
ACCESSION   DBNGTT010000427 DBNGTT010000000
VERSION     DBNGTT010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_613107"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="AC16BC_09650"
     CDS             complement(<1..108)
                     /locus_tag="AC16BC_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3241924.1"
                     /translation="MEPKYLVVEGPIGVGKTSLVTLLAEQLNGTPILEES"
     gene            complement(130..648)
                     /gene="folK"
                     /locus_tag="AC16BC_09655"
     CDS             complement(130..648)
                     /gene="folK"
                     /locus_tag="AC16BC_09655"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3241925.1"
                     /translation="MTEAMRAYIGVGSNVGDRFAHCLAAVEALNRLGGTRVVGCSRWY
                     ETEPVGAIAQAWFLNGAVGIETTLSPHELLARCRYIEGTRGRDRARETPGGPRPLDLD
                     ILLFGDQVIATPDLQIPHPRMAERAFVLAPLNDIAPRAIHPGLKRTIGDLRAGLADTH
                     QIRLFEAVPRSA"
     gene            complement(813..2048)
                     /locus_tag="AC16BC_09660"
     CDS             complement(813..2048)
                     /locus_tag="AC16BC_09660"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR3241926.1"
                     /translation="MASSYIQRTFAERIGGAKFGKSTKIYKFEKIKRAKAAAQKAHPG
                     IELLDFGVGEPDDMAFPGVVEALQREAARWENRGYADNGIAEFKDAAARYLDRVFGAS
                     GIAPQTEVIHSIGSKPALAMLPACFINPGDVAIVTVPGYPVMATHTKWYGGRVVELPL
                     TQERNFLPDLGALKSADLKRAKLLYLNYPNNPTGASATREFYQGVITFAKKHKLIVVV
                     DAAYASLTYGEPPFSFLSLPGAKDVGVELHSLSKAYNMTGWRLGFVAGNPLVVSAFGM
                     VKDNCDSGQFKAIQRAGIYALEHPEITAEIGRKYERRLRRMVDALGAVGFDVTMPRGS
                     FFLYTGAPKAVKGGRKFKSAEEVSEFLITEKLISTVPWDDVGRYLRFSATFLAKDETD
                     ERRVLAELKSRLSDLGLVF"
     gene            complement(2076..2798)
                     /locus_tag="AC16BC_09665"
     CDS             complement(2076..2798)
                     /locus_tag="AC16BC_09665"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3241927.1"
                     /translation="MMYGRLDGDVIRLIEGDLFGAYRVTERTVPLASARLLTPCLPTK
                     IVAIGVNYKDHAAEFKKDLPEEPLMFLKPPSALLAPEEAIVYPEGMTRRMDFEGELAV
                     VIRKRIRHATLEQATAAILGYTCCNDVTARDLQKKDGQWSRAKGFDTFCPLGPVIATD
                     LDPSQLRIETRLNGEVKQDAPVSAMIFDVPTLISRVSAVMTLMPGDVITTGTPSGVGP
                     MKPGDVVEVHIAGIGTLRNRVV"
BASE COUNT          494 a          952 c          850 g          503 t
ORIGIN      
        1 ggattcttcc aggatcggcg tgccgttgag ttgctccgcg agcaacgtca ccaggctcgt
       61 cttgccgacg ccgatcgggc cttcaacgac caggtacttg ggctccatcc gtcgctcgcc
      121 gaatcccggt cacgcggacc gcggcaccgc ctcaaacaac cgaatctgat gggtatccgc
      181 gagccccgcg cgcaagtcgc cgatggttcg cttgagcccg ggatggattg cgcggggcgc
      241 aatgtcgttg agcggggcga gcacgaacgc gcgctccgcc atgcgcgggt gcgggatctg
      301 taggtcgggc gtcgcaatca cctggtcgcc gaataacagg atgtccaaat ccaatggccg
      361 aggtccgccg ggagtctctc gcgcgcggtc ccgtccgcgc gtcccttcga tgtagcggca
      421 tctcgccaac agctcgtgcg gcgacagcgt ggtctcgatg cccaccgccc cgttgagaaa
      481 ccaggcctgg gcaatcgcgc ccaccggctc ggtttcatac cagcgcgaac agcccaccac
      541 ccgggtcccg cccaggcgat tgagcgcctc gaccgcggcc agacaatgcg cgaaccgatc
      601 ccccacgttc gatcccacgc cgatataggc ccgcatggct tcagtcatgg ttagcgcttt
      661 gtgatcctgt cgaatgcacc gcagatatca gcctgttcag gaggcggggg atgcaaggcg
      721 ccgcgagaac cggagcggag cgtactctgt gtgtacgtga gcaccggaag cgcagcggca
      781 acgcagcaga cgcccctcct ggacaggctc tgctaaaaca ccaagcccag gtccgacaac
      841 cgactcttca actcagccag cacccgccgc tcatccgtct cgtccttcgc caggaacgtg
      901 gcggagaacc gcagataccg acccacgtcg tcccacggca cggtggagat cagtttctcc
      961 gtgatcagaa actcggacac ctcttccgcg ctcttgaact tccggccgcc tttgaccgcc
     1021 ttgggcgcgc ccgtgtacag gaagaacgag ccgcgcggca tggtcacgtc gaaccccacc
     1081 gcgccgagcg cgtccaccat gcggcgcaag cggcgctcgt acttgcggcc gatctcggcc
     1141 gtgatctcgg ggtgctccaa ggcatagatc cccgcgcgct ggatcgcctt gaattgcccc
     1201 gagtcgcagt tgtccttgac catgccgaac gcggacacga ccaacggatt gcccgccacg
     1261 aacccgaggc gccagccggt catgttgtac gccttggaca atgagtgcag ctcaacgccc
     1321 acgtccttgg ccccgggaag cgacaagaag ctgaacggcg gttcgccata cgtcagagac
     1381 gcgtaggccg catccaccac cacgatcagt ttgtgttttt tcgcgaacgt gatgacgccc
     1441 tgatagaact cgcgggtggc ggacgcgccc gtggggttgt tggggtagtt caggtacaag
     1501 agtttggctc gcttgaggtc cgcggacttg agcgcgccga ggtccggcag aaagttgcgc
     1561 tcctgggtga gcggcaattc caccacccgg ccgccgtacc acttggtgtg ggtggccatc
     1621 accggatacc cgggcacggt gacgatcgcc acgtcgccgg gattgatgaa acacgccggc
     1681 agcatggcca gggcgggttt ggagccgatc gaatggatca cctcggtctg cggggcgatg
     1741 cccgatgcgc cgaacacgcg atccaaatag cgcgccgcgg cgtccttgaa ctccgcgatc
     1801 ccgttgtccg cgtatccacg gttctcccac cgcgcggctt cgcgctgcag cgcctcgacc
     1861 acgcctggaa acgccatgtc gtccggctcg cccacgccga aatcgagcaa ttcgatcccg
     1921 gggtgcgcct tctgcgcggc ggctttggcg cgcttgatct tctcgaactt gtagatcttg
     1981 gtggacttgc cgaacttggc cccgccgatc cgctcggcga atgttctctg aatatacgat
     2041 gacgccatac ggttcctcgt tctttcccaa cctcgttaca ccacgcggtt tcgcaacgta
     2101 ccgatgcccg cgatatgaac ttccacgaca tcgcccggct tcatcggccc caccccggac
     2161 ggggtgcccg tggtgatcac gtcgccgggc atcaaggtca tgaccgcgga cacgcggctg
     2221 atgagggtgg gcacgtcgaa gatcatggcc gagaccggcg cgtcctgttt gacctcgccg
     2281 ttcaggcgcg tttcaatgcg caactgcgac ggatcaaggt cggtcgcgat caccggcccc
     2341 aaggggcaga acgtatcgaa ccccttggcc cggctccact gcccgtcttt tttctgcaga
     2401 tcgcgcgccg tgacgtcgtt gcagcaggtg tagccgagaa tggccgccgt ggcttgttcc
     2461 aacgtggcgt ggcgtatgcg tttgcggatc accaccgcca gttcgccctc aaagtccatc
     2521 cgccgcgtca tgccttccgg gtacacgatc gcctcctccg gcgccagcag tgcgctcgga
     2581 ggcttgagga acatcaacgg ctcctcgggc agatctttct tgaactccgc ggcgtgatcc
     2641 ttatagttga cgccgatcgc cacgatcttg gtcggcaggc acggcgtcag cagccgggcg
     2701 gacgccaacg gcacggtgcg ctcggtcacc cgatacgccc cgaacaagtc gccctcgatc
     2761 aagcgaatga catcgccgtc gaggcgcccg tacatcact
//
LOCUS       DBNGTT010000428         1824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1506881, whole genome shotgun sequence.
ACCESSION   DBNGTT010000428 DBNGTT010000000
VERSION     DBNGTT010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1506881"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..401
                     /locus_tag="AC16BC_09670"
     CDS             111..401
                     /locus_tag="AC16BC_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241928.1"
                     /translation="MVTNYEVDAFISSLRDEGAEVQGLVKNFLKVLDDAHNPDLAPAR
                     RRLRELMQRDAEIGKHQQVWPLLAPWLAEVAAPAKPGKPKGTVIKEIGVAPP"
     gene            complement(414..>1824)
                     /gene="ligA"
                     /locus_tag="AC16BC_09675"
     CDS             complement(414..>1824)
                     /gene="ligA"
                     /locus_tag="AC16BC_09675"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR3241929.1"
                     /translation="QPLANPRNAAAGAVRQLDPSVTAQRRLKFMPYGLGVIEGAAPTT
                     HWETLELLTRLRFPVSPHNERLADLDAVWAFAQKWEARRDELDYDTDGTVIKIDDLEL
                     QRELGEVSRRPRWAIAYKFPAEEATTRVQDIVLNVGRFGSLNPVAMLDPVQVGGVTVR
                     KATLHNEDIIAKKDVRIGDWVVVRRAGEVIPEIVKSIPERRTGDERPFVAPSTCPVCG
                     GTVIRPPGEVSSRCVNAACRAQLEQRVKHFVSKGAMDIRGMGEQWALRLLAEGYIAEV
                     ADIYHLTKAQLLKLERVKEKTASNLLEAINASRGRPFPRVLYALGIQHVGEVTAEKLA
                     ETFRTMDALMAADEDAISRARDVGPITARIVHAFFQEPKNRKLIDRLRRAGLHMEYEA
                     AAGPLVGKTFVLTGSLASLTRGQAQDKIRAAGGNVTDSVSRKVDYVVVGDDPGSKLEK
                     AKKLGIKILDESELLSLLR"
BASE COUNT          308 a          584 c          595 g          337 t
ORIGIN      
        1 aaacgagaac gtttgccgtg gcggatctgc cctggccgga attggcgttc gacagtacaa
       61 aacacgcatt gaccgactat ttgaaacacc acacacgcta aaggagccgc atggtcacga
      121 attacgaagt ggacgcgttt atcagctcgc tgcgggacga aggggccgag gtgcagggcc
      181 tggtcaagaa ttttctcaag gtcctcgacg acgcgcacaa tcccgacctg gccccggccc
      241 gtcggcgatt gcgggaattg atgcagcgcg acgcggagat cgggaaacac caacaggtgt
      301 ggccgctgct cgcgccgtgg ttggccgaag tggcggcgcc ggccaagccg ggcaagccga
      361 agggaacggt cattaaagaa attggcgtgg cgccgccgta agggcggtgg ttgctaccgc
      421 aacagcgaga gcagctccga ttcgtccagg atcttgatgc caagcttctt ggctttctcc
      481 agcttggacc ccggatcgtc gccgaccacc acgtaatcca ccttccggct caccgagtcc
      541 gtgacgttcc cgcccgcggc gcggatcttg tcctgggctt ggccgcgcgt gagtgaggcg
      601 agcgagccgg tcaacacgaa ggtctttccg accaacggac cggccgcggc ttcgtactcc
      661 atgtgcaagc cggcgcgacg cagccggtcg atcaacttcc ggtttttggg ttcttgaaag
      721 aacgcgtgga cgatgcgcgc ggtgattggt cccacgtcgc gcgctcggga gatcgcgtcc
      781 tcgtccgcag ccatcaacgc gtccatggtc cggaacgtct ccgcgagctt ttccgcggtc
      841 acctcgccca cgtgctgtat ccccaacgcg tacaacacgc ggggaaaggg cctgccccgg
      901 ctggcattga tcgcctccag caggttcgac gcggtcttct ctttgacccg ttcgagtttc
      961 agcagctgtg ccttggttaa atggtaaatg tccgcgacct cggcgatgta tccctcggcg
     1021 agcaggcgga gcgcccactg ttcgcccatg ccgcgaatat ccatggcccc tttggagaca
     1081 aagtgtttca cgcgctgctc gagctgcgcg cggcacgcgg cattgacgca gcgcgacgac
     1141 acctcgcccg gcggtcggat cacggtgccg ccgcagaccg ggcacgtgga tggcgcgaca
     1201 aagggccgct cgtcgccggt gcggcgttcg ggaatcgatt tgacgatttc cgggatgact
     1261 tcgccggcgc gccgcaccac cacccagtcg ccgatccgca cgtccttctt cgcgatgatg
     1321 tcttcgttgt gcagcgtggc cttgcgcacg gtcacgccgc cgacctggac cggatccagc
     1381 atggcgaccg ggttgagcga accgaagcga ccgacgttca gcacgatgtc ctggacgcgg
     1441 gtggtggctt cttccgccgg gaatttgtat gcgatcgccc agcgcggccg acgcgacact
     1501 tcgccgagtt cgcgttgcag ctccaggtcg tcgatcttga tgaccgtgcc gtccgtgtcg
     1561 tagtccaact cgtcccgacg agcctcccat ttctgagcga aggcccacac cgcgtccaag
     1621 tcggccaggc gttcgttgtg ggggctgacc gggaagcgca ggcgggtgag cagttccaag
     1681 gtttcccagt gggtggtcgg cgcggcgcct tcgatcacgc cgaggccgta cggcatgaac
     1741 ttcaagcggc gctgcgcggt gacgctcggg tccagctggc ggaccgcgcc ggccgcggcg
     1801 ttgcgcgggt tcgccagggg ttgt
//
LOCUS       DBNGTT010000429         3879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3040837, whole genome shotgun sequence.
ACCESSION   DBNGTT010000429 DBNGTT010000000
VERSION     DBNGTT010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3040837"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /gene="nikR"
                     /locus_tag="AC16BC_09680"
     CDS             <1..404
                     /gene="nikR"
                     /locus_tag="AC16BC_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HIR3241930.1"
                     /translation="QRFGVSIESNLLTRFDRVIEQKGYTNRSEAIRDLIRDSLVEQEW
                     DENREIIGTVTIVYNHEVRELSDTLTDLQHQYQKLFRSTLHIHLDEHNCLEVLVVQGR
                     GRDVKHVADRLIGTKGVKHGKFTMTTTGKDF"
     gene            618..1907
                     /locus_tag="AC16BC_09685"
     CDS             618..1907
                     /locus_tag="AC16BC_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016830.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HIR3241931.1"
                     /translation="MITRLYSLWWIGVVMGVMLGAGTTSTAAAGDVLEELEGLKSRLK
                     ALEDRDRDSDEAHADEAGHRPHPLHSLAKTTLDGDITMIAQATPSVAPSAQSEGTMSV
                     DLFLEHQVSDAGLVLLHLDVAQGQGFQFFPVFTAPNGNPTGANNDVETFDGQTAVHLD
                     QAYYHHHWLDERIEVTVGQYDPTAFFDTNAYANSERTQFIAPSFGTTPTLEFGGTGNF
                     YGFGGVLELRPAKRVDVILGVMEGDGDYREMFTRPWSIAEVDLDLDPWGREGTYRFFV
                     WENHRHHDPAFSLLPDARNRGLGVNFDQALTTHIGIWGRYGVQDGRVAQFDRAASLGV
                     QIGGGLINRPFDAFGMAYGLTMIGDPYTAAQALSGNPQFDANEGYLEAYYRCVLSGDG
                     ERIGVAVSPDVQYVTNAGGDRSIDPIVVYGIRFQAFF"
     gene            1924..2904
                     /locus_tag="AC16BC_09690"
     CDS             1924..2904
                     /locus_tag="AC16BC_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR3241932.1"
                     /translation="MFVNTRGRWLVGIGALAAMMMIGPADAADTGKKIKVLTTVAPIT
                     NIVRNVAGSNVELHGLIPEGSDSHTFEPVPSDVKYVANADLIIINGLHLETPTMHLVD
                     ANKKKGSVVVQLADQTIAKKEWVFDFSFPADKGDPNPHLWLNAAHGMKYAELVRNALV
                     KVDPANKAAYDANATAYLAKLKQLDEAIMATMKTIPEKNRKLVTYHDSWAYFCPRYGC
                     KVIGAVQPSDFAQPTPQDMVALINQLKAEAVPAIFGSEVFPSKVLDQIGREAGVNYVD
                     TLRDDDLPGGLDDPKHTYVGMMKENVETMARVLGGDASALKNIDPSNVGK"
     gene            3031..3279
                     /locus_tag="AC16BC_09695"
     CDS             3031..3279
                     /locus_tag="AC16BC_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241933.1"
                     /translation="MAAYLLLFSSAALVHAYAQDELTDPHGCSIGAWVQHANATGASA
                     PPLATLIGLSEFLPATHDTITESAVFVVASRGPPRSSL"
     gene            3375..>3879
                     /locus_tag="AC16BC_09700"
     CDS             3375..>3879
                     /locus_tag="AC16BC_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241934.1"
                     /translation="MRGTAGQQFVMWCLGLVTLWAGPVSAADDESIQRLEQELAETRS
                     AVRALETAQSTAPGKGLALPDISLDGLFAAAASTAADLESLETGAHDPAQRGFTVQNL
                     ELTLGGSVDPYFTAVSHIVLQIDRNGESTLEVEEAYLTSTALPANLQVRAGQFFTQFG
                     RLNPTHPH"
BASE COUNT          778 a         1176 c         1181 g          744 t
ORIGIN      
        1 tgcagcggtt cggcgtgtcg atcgaatcca acctcctgac ccgcttcgac cgggtcatcg
       61 aacaaaaagg ctacaccaac cgctcggaag cgattcgcga cctgatccgc gattcactcg
      121 ttgagcaaga gtgggacgag aatcgcgaga tcatcggcac ggtcacgatc gtctacaacc
      181 atgaagtccg cgagttgtcc gacacgctta ccgaccttca gcaccaatac cagaaactct
      241 tccgctccac cctccacatc cacctggacg agcacaactg cctcgaggtt ctggtggtcc
      301 agggccgtgg ccgagacgtc aaacacgtgg ccgatcgcct gatcggcacc aaaggcgtca
      361 agcacggcaa attcaccatg acgaccacgg gaaaagattt ctagtcccca gtcgtgatcg
      421 ttttccgtct cgaaacagcc gctaacttca ttatctaaat tcgtgcgggt cggtctgggg
      481 ggggcggttg cggctgtttc attggtaaca cgaaaaatat gtttattgac acgaaatata
      541 cttcgtgcta tttatgttaa gcaagtagca cggcattggt gacggctgag accttggcgc
      601 ttttccggat gacgatgatg attacgcgac tgtactcatt gtggtggatt ggcgtggtga
      661 tgggagtcat gctcggcgcg gggaccacca gtaccgccgc ggctggcgac gtccttgagg
      721 aactggaagg gctgaaatcg cgcctgaagg cgctcgagga ccgggaccgc gactcggacg
      781 aggcccatgc cgatgaggcg ggtcacaggc cgcacccgct ccactccctt gcgaagacga
      841 cgctcgacgg cgacatcacg atgatcgctc aagcgacccc gtcagttgct ccaagcgcgc
      901 agtcagaagg caccatgtcg gtggatttgt tcttggaaca ccaggtgagc gacgccgggt
      961 tggtgttgtt acacctggac gtggcccagg gacaaggctt tcaattcttc ccggtgttca
     1021 ccgcgccgaa cggcaatccg accggtgcga acaacgacgt tgaaacgttc gacgggcaga
     1081 ctgcggtcca cctggatcag gcgtattacc accatcactg gctcgatgag cgcatcgagg
     1141 tgaccgtcgg tcaatacgac cccacggcat ttttcgacac caatgcctac gcgaatagtg
     1201 agcgcacgca gtttatcgcg ccgagttttg ggacgacccc gaccctcgag ttcggaggga
     1261 ccggcaactt ctacgggttc ggaggcgtgt tggaactccg gccggcgaag cgcgttgatg
     1321 tgatcctcgg ggtgatggaa ggcgacggag attaccgcga gatgtttacc aggccgtggt
     1381 ccatcgcgga ggtcgatctg gacctcgatc cttggggacg cgagggcacg taccggttct
     1441 ttgtctggga aaatcatcga caccacgatc cggcattctc gttgctcccc gatgcacgta
     1501 atcgcggatt gggcgtcaat tttgatcaag ccctgaccac ccacatcggc atctggggcc
     1561 ggtacggggt gcaggacgga cgggtggcgc agtttgaccg cgcggcttcg ctgggcgtgc
     1621 agatcggcgg cgggctcatc aatcgaccgt tcgacgcctt cgggatggcc tacggcctga
     1681 ccatgatcgg ggatccctac acggcggctc aggccttgtc cggcaatccg caattcgatg
     1741 cgaacgaagg ctatttggag gcgtactacc ggtgcgtgct gtcaggggac ggtgaacgga
     1801 tcggggtggc ggtgtctccg gacgtgcagt acgtgacgaa cgcaggtggg gatcggtcga
     1861 tcgacccgat cgtggtgtac ggaattcggt ttcaggcgtt tttctaatga tgggaggtca
     1921 cgcatgttcg tgaacacacg gggtcgatgg cttgtcggga tcggtgcgct ggccgcgatg
     1981 atgatgattg ggcccgcgga cgcggcggac accggaaaga agatcaaggt gctgaccacg
     2041 gtggcgccga tcacgaacat cgtgcgcaat gtggcgggtt cgaacgtcga attacacggc
     2101 ctgatcccag agggttcgga ttcccacacc ttcgagccgg tgccgagcga tgtgaagtac
     2161 gtggcgaatg ccgatctgat catcatcaac ggactgcacc tcgagacccc gaccatgcac
     2221 ttggtcgacg ccaataagaa gaaggggtcc gtggtcgtcc aactcgcgga ccagaccatc
     2281 gccaagaagg agtgggtctt tgatttctcg tttcccgcgg acaagggcga tccgaacccg
     2341 cacctgtggc tcaatgccgc gcacggcatg aaatacgcgg aactcgtgcg caacgcgctg
     2401 gtcaaggtcg atccggccaa caaggcggcc tatgacgcga atgcgaccgc gtacctcgcc
     2461 aaactcaaac agctcgacga ggcgatcatg gccaccatga agaccatccc ggagaagaac
     2521 cgcaaactcg tcacctacca tgactcgtgg gcgtacttct gcccgcgcta cggctgtaaa
     2581 gtcatcggcg cggtgcaacc gtcggacttc gcccagccca cgccccagga catggtggcg
     2641 ctgatcaatc agctcaaagc cgaagccgtg cccgcgatct tcgggtccga ggtgttcccc
     2701 agcaaggtgt tggatcagat cggccgcgaa gccggagtca actacgtgga taccctgcgg
     2761 gacgacgatc tgccgggcgg gttggacgat cccaagcaca cctacgtcgg catgatgaag
     2821 gaaaacgtgg agaccatggc gcgggtattg ggcggggatg cctcggcgct gaagaacatc
     2881 gatccgagca acgtcggaaa gtaggtcgcc ataacacggc gacccttgca aggccggaca
     2941 gactgtggta cctctaaccc cggtctgatg aacaacccgt tccttcgtct gcgcgcccat
     3001 cttccagggc ggtcgctgtt ttggaccgcc ttggccgcgt atctgctgct gttttcgtcc
     3061 gccgcgctcg ttcacgcgta cgcgcaagac gagctgactg acccccacgg atgctccatc
     3121 ggcgcgtggg tacagcacgc caacgcgacc ggggcctccg ctccaccgct cgccaccctc
     3181 attggcctca gcgaattcct cccggcgact cacgacacga tcactgaatc ggctgttttc
     3241 gtcgtcgcct cccgcggtcc gccgcgctct tctctctaat ctcctcaacc acctaaccga
     3301 atcggaatac cacctcgcgt gcgcggatga cctccgcgcg gcttcgtcgt gtgttccgtg
     3361 tccatggagg agtcatgcgg ggaacagcgg gtcagcaatt cgtcatgtgg tgtcttggcc
     3421 tcgtcacctt gtgggccggg ccggtttcgg cggctgatga cgaatcgatc caacgtctcg
     3481 agcaagaatt ggccgaaaca cggtccgccg ttcgcgcgtt ggagaccgcg caatccacgg
     3541 caccgggcaa aggcctcgca ctcccggaca tttcgctgga cgggttgttc gcggccgcgg
     3601 cgtccaccgc ggcggatctg gagtcgttgg agaccggcgc ccacgatccc gctcagcggg
     3661 gcttcacggt tcagaatctg gaactcacgc tcggagggag cgttgatccc tacttcaccg
     3721 ccgtcagcca catcgtgttg cagatcgacc gcaacggcga gagcacgctt gaagtcgagg
     3781 aagcgtacct gacatccacc gcgctgcccg ccaatctgca agtgcgcgcc ggacagttct
     3841 tcacccaatt cgggcggctc aaccccacgc atccgcata
//
LOCUS       DBNGTT010000430         2473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_230870, whole genome shotgun sequence.
ACCESSION   DBNGTT010000430 DBNGTT010000000
VERSION     DBNGTT010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_230870"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="AC16BC_09705"
     CDS             <1..614
                     /locus_tag="AC16BC_09705"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR3241935.1"
                     /translation="AALGLQVADHDVRFVQDDWESPSLGAWGLGWEVRLDGMEITQFT
                     YFQEIGGIELNPISAEITYGLERIAMYLQQVDSVFDLQWTDRVRYGDVHHETEVQGSR
                     YNFDRAHVPSLFTEYDLHEQEAKRLLADDLVLPAYDHCMKTSHLFNVLDARGAISVTE
                     RATYIARVRGLARACAEGWLKQREAKGFPLLGRETKSKARSGR"
     gene            618..>2473
                     /gene="glyS"
                     /locus_tag="AC16BC_09710"
     CDS             618..>2473
                     /gene="glyS"
                     /locus_tag="AC16BC_09710"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR3241936.1"
                     /translation="MATRTPPTADLLFEIGTEELPWTVIRGTIAHLDGATRAAFERAR
                     LSTRSVQVYGTPRRLVVVAEGVAARQAEATTQVVGPPASAAFDAEGKPTRAAEGFAKS
                     QNVAVADLTVVTTEKGRYVAVARSEERKAASAVLRELLPTLISGLSFPRAMKWEPGGA
                     RFARPIRWMVALFGGKSIPIRYGGVASGPMTRGHALSAPKPFRAPATWAAFSRALERR
                     GVIVDHARRRSAIAEQLAEAARGAGGRLVEDDDLLEQAAFLTESPCAVAGAFDRRYLA
                     LPREVIVTVLKEHQGYFSLVDKKGEPLPAFIAVSNVKPAARAMAAIRAGYERVIRARL
                     DDAKFYFDQDQKEALADRVVRLKGVVFQEKLGTVYEKVERLAARARQLAPRFEVDADQ
                     AERAARLCKADLTTGMVREFPELQGVMGREYARRQREPEAVAEAIGDHYRPRFAGDRL
                     PAHPLAQLVAVADKVDTIVRCFGAGLIPTGSQDPYALRRQALGVVQILRERPVVTLSA
                     LIASGAQSGTDHGTILEFFCQRIESQAKAEGFRADLINAVLDVPSAADAPHAAFRRLA
                     ALTAFSQRPAFESLMTACKRVMNILPEPCPAAIDPSRFTHDAERRLHAETQRA"
BASE COUNT          381 a          812 c          912 g          368 t
ORIGIN      
        1 tggcggcgct gggcctccag gtcgcggacc acgacgtgcg gttcgtgcag gacgactggg
       61 agtcgccgtc gctcggggcg tgggggctgg gctgggaagt gcgattggac gggatggaga
      121 tcacccagtt cacgtatttt caggagatcg gcgggatcga gctcaacccg atttccgccg
      181 agatcaccta cggcctcgaa cgcatcgcca tgtacctgca gcaggtggac agcgtgttcg
      241 acctgcaatg gaccgaccgg gtgcgctacg gcgacgtgca ccacgagacc gaagtccagg
      301 ggtcgcgcta caacttcgac cgcgcccacg tcccgtcgtt gttcacggag tacgacctgc
      361 acgagcagga agccaaacgc ctgctggccg acgacctggt cctgcccgcg tacgatcact
      421 gcatgaagac ctcgcacctg ttcaacgtgc tggacgcgcg cggcgcgatc agcgtgaccg
      481 aacgcgccac gtacatcgcc cgcgtgcggg gcctggcgcg cgcctgcgcg gaaggctggc
      541 tcaagcagcg ggaagccaaa ggattcccat tgctggggcg tgaaacgaag tcgaaggcgc
      601 gatcgggacg gtaggctgtg gcgacgcgca ccccaccaac cgccgaccta ttgtttgaga
      661 tcggcaccga ggagttgccc tggaccgtga tccgcgggac gatcgcgcac ttggacggcg
      721 ccacgcgcgc cgcgttcgaa cgggcgcggc tgtccacgcg gtcggtgcag gtctatggca
      781 cgccgcggcg gttggtcgtc gtcgcggaag gcgtggccgc gcgccaggca gaagccacca
      841 cgcaggtggt gggcccgccc gcgagcgcgg cgttcgacgc cgaaggcaag cccacccggg
      901 cggccgaggg cttcgccaaa tcgcagaacg tggcggtcgc ggatctgacc gtggtcacga
      961 ccgagaaggg gcggtacgtg gcggtggcgc gatcggagga gcgcaaggcc gcgtccgcgg
     1021 tgctgcggga gctgctgccc acgctcatca gcgggctctc gttcccgcgc gccatgaaat
     1081 gggagccggg cggcgcgcgg tttgcccggc ccatccgctg gatggtcgcg ctcttcggcg
     1141 ggaagtccat cccgatccga tacggcgggg tcgcgtcggg acccatgacg cgcggccacg
     1201 cgctgagcgc gccgaaaccg tttcgggcgc ccgcgacctg ggccgcgttt tcccgggccc
     1261 tggagcgtcg cggcgtgatc gtggaccacg cgcggcgccg gtcggcgatc gccgagcaac
     1321 tggccgaggc cgcgcgcggg gcgggcggcc gtctggtcga ggacgatgat ctgctcgaac
     1381 aagccgcgtt cctcaccgag tcgccttgcg cggtcgcggg cgcgttcgac cgccgctacc
     1441 tggcgttgcc ccgcgaagtg atcgtgaccg tgctgaagga gcaccaggga tatttctcgc
     1501 tggtggacaa gaagggcgag cccttgcccg cgttcatcgc ggtgagcaac gtgaaacccg
     1561 cggcccgcgc catggccgcg attcgcgccg ggtacgagcg ggtgatccgc gcgcgcctgg
     1621 acgacgcgaa gttttatttc gatcaggatc agaaggaagc gctggccgac cgggtggttc
     1681 ggctcaaggg cgtggtgttc caggagaagc tcgggaccgt gtacgagaag gtggagcggc
     1741 tggcggcccg ggcgcggcaa ctcgcgccgc ggttcgaggt ggacgcggat caggccgaac
     1801 gcgccgcgcg gttgtgcaag gcggatttga ccaccggcat ggtgcgcgag ttccccgagt
     1861 tgcagggggt catggggcgc gaatacgcgc gacggcagcg cgaacccgag gccgtggccg
     1921 aagccatcgg cgaccattac cggccgcggt ttgccgggga tcggttgccc gcgcatccgc
     1981 tcgcgcaact ggtggccgtg gcggacaagg tggacaccat cgtgcgctgc ttcggcgcgg
     2041 ggttgatccc cacggggtcg caggacccgt acgcgctgcg acgtcaggcc ttgggcgtgg
     2101 tgcagatcct gcgcgagcgt ccggtggtca cgctctcggc gttgatcgcg agcggcgcgc
     2161 agtccggcac ggaccacggc accatcctgg agttcttctg ccagcgcatc gagtcccagg
     2221 ccaaggccga gggattccgc gcggatttga tcaacgcagt gctggacgtg ccgtccgcgg
     2281 ccgacgcccc gcacgcggcg ttccgccggc tggccgcgct caccgcgttt tcgcaacggc
     2341 ccgcgttcga gtcgttgatg acggcctgca aacgggtcat gaacattctg cccgagccgt
     2401 gccccgccgc gatcgacccc tcgcgattca cgcacgacgc ggagcgccgc ctgcacgcgg
     2461 aaacgcagcg ggc
//
LOCUS       DBNGTT010000431         4987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1125147, whole genome shotgun sequence.
ACCESSION   DBNGTT010000431 DBNGTT010000000
VERSION     DBNGTT010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1125147"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..568)
                     /gene="gspM"
                     /locus_tag="AC16BC_09715"
     CDS             complement(20..568)
                     /gene="gspM"
                     /locus_tag="AC16BC_09715"
                     /inference="COORDINATES: protein motif:HMM:NF016493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HIR3241937.1"
                     /translation="MKARLSGWWRERNERERMMLGVGAVAVVALAVYGLVVDPLLERS
                     ARLDRKITQRQEDLDRVTKLAGQYRVAADQVAVLNQRMSAAGSFSLLSFLEESASAQQ
                     VRNRLASIRPQPAQTLAPYREVAAEVKLEAITLPQVVGYLEALDRAPQRIRIKSLRLK
                     TRYADPKLLDGSFVVSTYERMS"
     gene            complement(565..2064)
                     /locus_tag="AC16BC_09720"
     CDS             complement(565..2064)
                     /locus_tag="AC16BC_09720"
                     /inference="COORDINATES: protein motif:HMM:NF022554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HIR3241938.1"
                     /translation="MRGIGFDIGGSSITAAVVNAGLKGVRLEVVRSVRVSGIEEERAR
                     ALRSLKLRAAPAAIAVPVHQVSGRPVTLPFAQQTKWDAALPSELEGQIPFELDEVVID
                     GALMEREGAQSRVLAVALPKTTLRARLEEAARGGVDPKLVTVEAEALAAAAGLWLPVQ
                     GDAALVHLDERAVTLVLVARGRVRAMRAVLWDGAAARATVAVACGVPEAALEAVAAGD
                     GPPDVSGRAVADALVSTLKPALDDMARTLRADRVESSRRVAAIAVSGRWSAIPEVGEA
                     VAAALGLDGAPWPSVPVSGGERPLGPIALAAGLGLLAVRGADRLNFRRGEFIYGRERA
                     GLRRRIVALVALAVFALAAAGVDGWLRVAFKQRRWADIDGRVRAAFQEALPNTAIVSE
                     PDQLQAGIDTLAKQRAFLGGRLGVLDVLLALTDAIPPESGIAVSDLSIDQDKVRLEAE
                     TASFDWVNKIEGSVAKSPVVKSVTVSDAKTTADQSKVRFIMTVVLAEGV"
     gene            complement(2067..2960)
                     /gene="gspN"
                     /locus_tag="AC16BC_09725"
     CDS             complement(2067..2960)
                     /gene="gspN"
                     /locus_tag="AC16BC_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HIR3241939.1"
                     /translation="MTPPRANRRISWGALVAYAFYTLAVFLVFLVWQFPVEALQTRLL
                     AEIEQRTRAMVTVQEREWLFPLGVSWRGVRVVPRAAPERVIDLETVRVRVALLPLISR
                     RVEADLAWEAYGGKVRGGFTVRREGDGVRTSLDQFGQGFDLARIPGLPSGSWSGALQV
                     DLNGRWLNDAWWLGEGTGSAELSGAKIDGLVISGFPVNGIEFDALTAQIALKGGTVTL
                     QRLAGRGPLGTVSGDGTVLVRAPWTESVINVTLRLDPSAAAKSRVPMLALTEAQGGAV
                     TVRISGRLAKPGVIVNGVPIA"
     gene            complement(2988..3752)
                     /locus_tag="AC16BC_09730"
     CDS             complement(2988..3752)
                     /locus_tag="AC16BC_09730"
                     /inference="COORDINATES: protein motif:HMM:NF023044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HIR3241940.1"
                     /translation="MNTWIWSAMRSSRASDVVQTRSVFRNAESAWLRDQGGPQRLSVA
                     PLAQGGFTLLEVVVAIGILAVVMTIVYGAFSRSMDVTRETAEVTDRVRQVQIVTERLV
                     DELSAAYWIDEKRARFVGTKDTTSSEDARLDRLVWSTFAHRRYVGDSPESDVAELTYR
                     VEPSPETTTGRLMRDELINPLAAASWATHSDELADGVSEFRLRYLSGGEWVDEWDGAQ
                     RKGLPEAVEVTVALAGSGTAPAERVRTIVPLPLARR"
     gene            complement(3706..4068)
                     /gene="gspI"
                     /locus_tag="AC16BC_09735"
     CDS             complement(3706..4068)
                     /gene="gspI"
                     /locus_tag="AC16BC_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HIR3241941.1"
                     /translation="MAQSGFTLLEVMIALAIIAVAFVALLGLRNRDIVMHDRARAVIE
                     ATALAQQRLSETLVGAFPDVGTSEGQFDAGHARYAWRQSVSPTPFDFAREVRVSVIWD
                     PPQNEHVDLVSYAFQQGK"
     gene            complement(4149..4616)
                     /locus_tag="AC16BC_09740"
     CDS             complement(4149..4616)
                     /locus_tag="AC16BC_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241942.1"
                     /translation="MVFILALMAVLVTPALHRFAGGDARSASRELTGLVSALVQDSVA
                     THTIQRLYYDLDQREYWVTLLTPVGGVLEESQPVGVKRRLPSDVRFEDVVTAHQGLIT
                     SGTAFTQFFPSGAVTRTTIHLKDDADARYTVLVNPVTGRVAIKDGYIDVVGAS"
     gene            complement(4757..>4987)
                     /gene="gspG"
                     /locus_tag="AC16BC_09745"
     CDS             complement(4757..>4987)
                     /gene="gspG"
                     /locus_tag="AC16BC_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HIR3241943.1"
                     /translation="TEQGLDALVNKPTSSPVPERWRTGGYIPKVPKDPWNHAYVYLSP
                     GTHGDFDLLSFGGDGERGGEGKDADLENWDLK"
BASE COUNT          872 a         1851 c         1511 g          753 t
ORIGIN      
        1 ctcgccgcgg gccgcgcggt caggacatgc gttcatacgt ggacaccacg aacgagccgt
       61 ccagaagctt ggggtccgcg taccgcgtct tgaggcgcaa cgacttgatc cggatgcgct
      121 gcggggcgcg gtcaagggct tccaggtatc cgaccacctg cgggagcgta atcgcttcga
      181 gtttgacctc ggccgcgacc tcgcggtacg gcgccaacgt ttgcgcgggc tgtggtcgaa
      241 tcgacgcgag ccggttccgg acttgctgag cggaggccga ctcctcgaga aacgagagca
      301 gcgagaagct cccggccgcg gacatccgct gattgaggac cgccacctga tccgccgcca
      361 cgcggtactg cccggcgagc ttcgtgaccc gatcgaggtc ttcctggcgc tgggtgatct
      421 tgcggtccag ccgcgcgctg cgctcgagta gaggatccac gaccagtccg tagaccgcga
      481 gggccacgac cgcgaccgct ccgaccccga gcatcatgcg ctcgcgctcg ttgcgctctc
      541 gccaccagcc cgacagacgc gccttcatac gccctcggcc aggacgacgg tcatgatgaa
      601 ccgcaccttg gactggtcgg ccgtggtctt ggcgtcgctg accgtgacgc tcttgaccac
      661 gggcgatttc gccaccgagc cctcgatctt gttgacccaa tcaaacgacg cggtttcggc
      721 ttcaagtcga accttgtcct gatcgatcga caggtccgag accgcgatgc cgctctccgg
      781 cgggatcgcg tcggtcaacg ccaacaacac gtccaacacg ccgagacggc cgcccagaaa
      841 ggcccgctgc ttggccagcg tatcgatccc ggcctggagt tgatcgggtt cgctgacgat
      901 cgcggtgttg ggaagcgcct cctgaaaggc cgcgcggacc ctgccgtcga tgtccgccca
      961 tcgccgttgt ttgaacgcga cccgcaacca tccgtcgacc ccggccgctg ccagcgcgaa
     1021 tacggcgagc gccaccagcg ccacgatccg ccgacgcagc cccgcgcgct cgcggccgta
     1081 gatgaattcc ccccgtcgaa agttgaggcg atccgcgccg cgcaccgcga gcaggccgag
     1141 ccccgcggca agggcgatcg gcccgagggg ccgctcgccg ccgctcaccg gcaccgacgg
     1201 ccacggcgcg ccgtccaggc cgagcgcggc cgccaccgct tccccgacct cggggatcgc
     1261 ggaccaccgt ccgctcaccg cgatcgcggc cacgcggcgc gacgactcga cccggtccgc
     1321 gcgcaacgtg cgcgccatgt cgtccagcgc gggtttgagc gtggagacga gggcgtcggc
     1381 caccgcgcgg ccggagacat cgggcgggcc gtcacccgcc gcgacggcct cgagcgccgc
     1441 ctcgggtacg ccgcaggcca cggctacggt cgcccgcgcg gccgcgccgt cccataacac
     1501 cgcgcgcatc gcacggacgc gcccccgcgc gaccaggacg agcgtgaccg cgcgttcgtc
     1561 gaggtgcacg agcgccgcgt cgccctgaac cggcaaccac aaacccgcag ccgcggccag
     1621 ggcttcggcc tccaccgtca cgagcttggg atccaccccg ccgcgggcgg cctcctcgag
     1681 ccgcgcgcgc aacgtggtct tcggcaacgc cacggccagc acccgcgact gcgcgccttc
     1741 gcgctccatc agcgcaccgt cgatcaccac ctcgtcgagc tcgaacggga tctggccctc
     1801 caattcggac ggcaacgccg cgtcccattt ggtctgctgc gcgaacggga gggtcaccgg
     1861 acgtccggac acctggtgga cgggcacggc gatggccgcc ggcgcggccc gcagtttgag
     1921 cgatcgaagc gcgcgggcgc gctcctcctc gattccggac acccgcacgg aacggacaac
     1981 ctccagtctg acgcccttga gccccgcgtt cacgaccgcg gccgtaatcg acgagccccc
     2041 gatatcgaac ccgatcccgc gcatggctac gcgattggca ctccgttcac gatcacgccg
     2101 ggcttggcca atcgcccgct gatccggacc gtcacggccc ccccctgcgc ttcggtcaat
     2161 gcgagcatgg gcacccgcga tttggcggcg gcggatggat cgaggcgcaa cgtgacgttg
     2221 atcacgcttt cggtccacgg cgcgcgcacc aggaccgtgc cgtcgccgga caccgtgccc
     2281 aacggcccgc ggcccgcgag ccgttgcagc gtgacggttc cgcccttgag ggcgatttgc
     2341 gcggtcagcg catcgaactc gatgccgttg accgggaatc cggagatcac caggccgtca
     2401 atcttcgccc ccgaaagctc ggccgagccc gttccctcgc cgagccacca cgcgtcattg
     2461 agccagcgtc cgttcagatc gacttgcagc gcgccgctcc acgagccgct tgggagtcct
     2521 ggaatccgag ccagatcgaa tccctgcccg aattgatcga gcgaggtccg caccccgtcc
     2581 ccttcacgcc gcaccgtgaa gccgccgcgg accttcccgc cgtaggcctc ccacgccaga
     2641 tcagcctcga cccggcggct gatgagcggc aggagcgcga cgcggacccg cacggtttca
     2701 agatcgatga cgcgttccgg cgctgcacgc gggaccacgc gcacgccgcg ccacgagacc
     2761 cccaggggga acagccactc gcgctcctgc accgtcacca tcgcccgtgt ccgttgttcg
     2821 atctccgcca acaaccgggt ctgcagggct tcgaccggga actgccagac caaaaagacc
     2881 aggaacaccg cgagcgtgta gaacgcatac gcgacaagcg ccccccacga aattcggcga
     2941 ttggcgcgcg gaggagtcaa tgaaacggac ccaggcctca gcacaagcta ccgccgagcg
     3001 agcggcaacg gcacaatggt ccgcacgcgc tcagccggcg ccgtccccga cccggccagc
     3061 gccaccgtga cctccaccgc ctccggcagc cccttgcgtt gggccccatc ccactcgtcc
     3121 acccactcac cgccgctcaa ataccgcaaa cgaaattccg acacgccgtc ggccagttca
     3181 tcggagtgcg tggcccacga ggccgccgcc aacggattga tgagttcatc ccgcatcaat
     3241 cgtccggttg ttgtttcggg agacggttcc acccggtagg tcaactccgc cacgtcggat
     3301 tccggactat cgcccacgta ccggcggtgc gcgaacgtgg accacaccaa tcggtccaag
     3361 cgcgcgtctt cggacgacgt ggtgtccttc gtgccgacaa agcgcgcccg cttctcgtcg
     3421 atccagtacg cggccgacag ctcgtcgacc aaccgttcgg tgacgatttg gacctgccgg
     3481 acgcgatccg tgacctcggc ggtctcgcgc gtcacgtcca tgctcctgga gaacgccccg
     3541 tacacgatcg tcatcaccac ggcaaggata ccaatcgcca ccacgacctc aagcaacgta
     3601 aagccgcctt gcgccaaggg cgcaactgat aatcgttgcg gtcctccctg atcgcgaagc
     3661 caggcgcttt ctgcgttccg aaacacgctc cgcgtctgca cgacgtcact tgccctgctg
     3721 gaacgcatag ctgaccagat ccacgtgttc attctgcggc ggatcccata tcacgctgac
     3781 ccgcacttcc cgcgcaaaat cgaacggcgt gggcgacact gactgacgcc atgcgtatcg
     3841 cgcgtgtcct gcgtcgaatt gcccctcgct ggtgcccacg tcgggaaacg cgcccaccaa
     3901 cgtctcgctc aaccgttgct gcgccaatgc cgtggcctcg atgaccgcgc gcgcgcgatc
     3961 gtgcatcacg atgtcgcgat tgcgaagccc gagcagcgcg acaaaggcca cggcaataat
     4021 ggccagcgca atcatgacct ccagtaaggt aaatccgctt tgcgccaacg gcgcaactcg
     4081 taatcgtccc cttccttgcg gatcgcgcag caacccgtac tgcgctcccc gtggtgtcag
     4141 actcctgctc acgacgcccc caccacgtcg atgtagccgt ccttgatcgc gacgcgtccc
     4201 gtcaccgggt tcaccaatac cgtgtaccgc gcgtccgcat cgtctttgag atggatggtc
     4261 gtgcgggtca cggccccgga cgggaagaat tgggtaaacg cggtgccgga cgtgatcagc
     4321 ccctggtgcg ccgtgaccac gtcttcgaac cgcacgtcgg agggcaaccg gcgcttcacc
     4381 cccaccggct ggctctcctc aagcaccccg ccgaccgggg tgagcaacgt gacccagtat
     4441 tcgcgttgat ccaaatcgta gtacaggcgc tgaatcgtat gcgtggccac cgagtcctga
     4501 acgagcgccg acaccagccc cgtcaactcg cgagaagccg accgcgcgtc gccccccgca
     4561 aagcggtgca acgcgggcgt caccagcacc gccatgagcg cgaggataaa gaccacgatc
     4621 gtgatttcga ggagcgtgaa acctgacgaa tcatggagac ggccccgggc cgggcaatcc
     4681 ccgcctgtcc cccgttgaca atggggggaa gggaggattt gaccttggcg tacccgcgag
     4741 aacggccgat cgaacgtcac ttcaggtccc aattctccaa atcagcgtct ttaccctcac
     4801 cgccccgttc cccgtcaccg ccgaaggaga ggaggtcgaa gtccccgtgc gtgccgggcg
     4861 acaggtacac gtaggcatga ttccagggat ccttgggcac cttcggaatg tatccgccgg
     4921 tgcgccaccg ctcgggaacc ggactcgagg tgggtttgtt caccaacgcg tccaacccct
     4981 gctcggt
//
LOCUS       DBNGTT010000432         1833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2274735, whole genome shotgun sequence.
ACCESSION   DBNGTT010000432 DBNGTT010000000
VERSION     DBNGTT010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2274735"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1223..>1833)
                     /locus_tag="AC16BC_09750"
     CDS             complement(1223..>1833)
                     /locus_tag="AC16BC_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3241944.1"
                     /translation="DGPCYRCLQPEPPPPHLVPSCAEGGVLGVLPGVIGTLQAAEAIK
                     LITGAGETLAGRLLLFDALRMTFRTIRLRRRCEEHAAVTRLIDYEELCNPVHNNTDIT
                     PTEVHSKLSNGDEIVVIDVREPHEWSAGHIAQATHIRLSELPQRLAEIPRDREVVMVC
                     RSGGRSENARRFLAQSGYTQVKNMVGGMMRWAREVDPSVRVA"
BASE COUNT          378 a          557 c          549 g          349 t
ORIGIN      
        1 accgccgatc ttagcgacta tggattgacg tatacgcgga tgctcgattc ccgtggtcct
       61 gtaacatttt tgcccaacga aaattcgtat tgtcttgtga cctatctaga gcccgagtta
      121 ccaccgcgtc ccacgaaaag cggctcgaag taggcaacga ggtgatcttc tgtcccggtt
      181 cctacaatct ctggaacggc tattcggggg cagcctcaga cccttagttt agattagatt
      241 agtctagttt aagggaagat taagtaatgt gtcgtcaacg cgatacggag cgaggaaccg
      301 cagcggccgc aggttcacgc catcgagtgt tgaagacctg tccgcacgtc ccacgtgggg
      361 tccgtccgtc gaggctcaac gtctgtccca cgtctcaatc aattgagcac cccctcagcg
      421 cgcaatcaac tcttttccag ctgtaccacg ccaactgaag catcttcttc cagaagctga
      481 ggccctgcca ccatctgcga aggcctctgc acgagtcaca gccggtgccg accggtggcc
      541 caccattgct tccggtgctg ggaaaggtgc cgccaatgac gccccaaaca atgacaacga
      601 ttacgataac aacgatccat ccccacatgg gtgcctccct ctgttcggac cgcgcgtttg
      661 gcttgtcccg gctgacacca cctgttttgg aactgccaag cgctttgggc gttactccgt
      721 ctctgaagaa atagtgtcga ttcacgcgaa aacgtttcaa aatcacggaa gacgaggaaa
      781 aggtgataga tctccaatac tcggaaagac cggcaaggga gagcagaaaa ccaattccgg
      841 ctccagcctg agttggccgt tgcgcgcgct caatacaggc caagaaccaa tcgaacgatg
      901 gataggaatg gtatcgcccc aatcgtgagg tgagctgtga gcgcgacata cgaaacagcg
      961 cgcccgcgga agccgtgata ggagaggatg accgcggctg tcgctgtaat gattcccagg
     1021 agcgagcagg cggcgatcca aaacaaagcg aggtacggac cgccgggtcc gaggttccgc
     1081 atccactccg gaaacaagac ctccgccgcg aacggggtcg cgagctccac ggcgagaaaa
     1141 agccaggcga ggcctgtgac gcagttgcgg cgcattcggc tcaacgtgcc tgcgaatgga
     1201 acgacttgtc cggcgtcaac cttcacgcca cccgcacgct cgggtccact tcccttgccc
     1261 agcgcatcat tccgcccacc atgtttttca cctgcgtgta gccggactgg gccaggaagc
     1321 ggcgggcgtt ttcgctccgt ccgccggagc ggcagaccat gaccacttcc cggtcgcgcg
     1381 ggatctcggc gagtcgttgc ggcagctcgg agaggcggat gtgcgttgcc tgcgcgatgt
     1441 ggccggcgct ccattcgtgc ggctcgcgca cgtcgatgac gacaatctca tcgccattgg
     1501 acagtttgct gtgaacctcg gtcggggtga tgtcggtgtt gttgtgcacg ggattgcaga
     1561 gctcctcgta atcgatcagt cgggtgacgg ccgcgtgctc ttcgcagcgt ctgcgaaggc
     1621 ggatggtgcg gaacgtcatc cgcaaggcgt cgaagagtag cagacgtccc gccagcgtct
     1681 ctccggcgcc ggtgatgagc ttgatggcct cggcggcctg cagggtcccg atcacgcccg
     1741 gcagcacgcc gagcacgccc ccctcggcgc acgacggcac gaggtgcgga ggcggcggct
     1801 cgggctggag gcagcggtag cacggcccgt ccg
//
LOCUS       DBNGTT010000433         2953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1636088, whole genome shotgun sequence.
ACCESSION   DBNGTT010000433 DBNGTT010000000
VERSION     DBNGTT010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1636088"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1195
                     /gene="mtnA"
                     /locus_tag="AC16BC_09755"
     CDS             104..1195
                     /gene="mtnA"
                     /locus_tag="AC16BC_09755"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR3241945.1"
                     /translation="MTATAHTPQPRFITVGWKDGRVRLLDQRRLPGEVVYLDCADYRD
                     VAEAIRTLAVRGAPAIGIAAAMGLALGARTLSGASADDFQSRLSKIADELAATRPTAV
                     NLFWALDRMKRVAAGHRHLQPEALVRRLIDESQAILDEDVAMNRTMGAIGAAFIHDGD
                     TVLTHCNAGALATGGYGTALGVIRAACESGKRIRVVADETRPILQGARLTAWELMQDN
                     IPVTLITDNMAGALMRQGRIQACVVGADRITANGDVANKIGTYGVAVLAKHHGLPFYV
                     AAPTSTLDLSLRSGADIPIESRNPREVTHVNGQAHIAPDGVAVDNPAFDVTPAELITA
                     IITEKGAFRPADIRRLDGDPESLAAHRLA"
     gene            complement(1309..2079)
                     /locus_tag="AC16BC_09760"
     CDS             complement(1309..2079)
                     /locus_tag="AC16BC_09760"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR3241946.1"
                     /translation="MKRMRDWMTGCGLAGVVLLGASPSWAQEEGLWKGNLELSYLQTS
                     GNTSSTTLAAGGKAERALDRAKLTGEFLAIYGEKETVASDKNWMGRLKYDRFVSSRAF
                     AYLAETVERNVLKGVEIRYTTQAGMGYEFIKTPSDLLKGELGVGYIRENPIALDPTDT
                     TPPIDPLHDLGYMTGRAYGEYNHAFDDKTRFLQTAEYLPGLKGGVGFLAKEESSFITN
                     LMGNFAWKVSYAVLYDSDPPAGFTRYDRVFKTSLLYTF"
     gene            complement(2138..2581)
                     /gene="mscL"
                     /locus_tag="AC16BC_09765"
     CDS             complement(2138..2581)
                     /gene="mscL"
                     /locus_tag="AC16BC_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR3241947.1"
                     /translation="MLKDFKAFILRGNVLDMAVGIIIGAAFGKIVTSLVSDVLMPPIG
                     LIAGRVNFADLFINLSDTAYPSLAAAKAAGAPTLNYGMFLNTVVDFLIVAAVIFVLVK
                     QFARLQRQPEPPAAAPTTKACPYCVSTIPLKASRCPQCTSEIKAA"
     gene            complement(2655..>2953)
                     /locus_tag="AC16BC_09770"
     CDS             complement(2655..>2953)
                     /locus_tag="AC16BC_09770"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR3241948.1"
                     /translation="AWRSEWAFRADVAVFLVAAPLGLWVGRSGVERALLIGSLFLVLI
                     AELVNTAIEAVVDRIGPDRHALSKKAKDVGSAVVLLALFNAGVIWAVILLPRFL"
BASE COUNT          537 a          980 c          942 g          494 t
ORIGIN      
        1 gccgcgcgac atggcgcgcg gcgggacgtg gcgtaatgcg aatgttttga caccgcgtcc
       61 gcgccaagcc tacaataggc ggattcggag aaggactctc gtcatgactg cgacagcaca
      121 cacgccacaa ccccggttta tcaccgtcgg ctggaaggac ggccgcgtgc ggctcctgga
      181 tcaacgccgg ctcccgggcg aggtagtgta tctcgactgc gccgattatc gcgacgtggc
      241 ggaggccatc cgcacgctgg cggtgcgcgg ggcgccggcc atcgggatcg cggccgccat
      301 ggggcttgcg ctcggggcga gaaccctgtc aggagcgtcg gccgacgact ttcagagccg
      361 gttgtccaag atcgccgacg aactcgccgc gaccagaccg accgcggtga atctgttctg
      421 ggccctggac cggatgaagc gcgtggccgc gggccaccgg caccttcaac ccgaggccct
      481 ggtccggcga ttgatcgacg agtcgcaggc gattctggac gaggacgtgg ccatgaaccg
      541 gaccatgggc gcgatcggcg ccgcgttcat tcacgacggc gacaccgtgc tgacccactg
      601 caacgcgggc gcgttggcca ccggcgggta cggcaccgcg ctcggcgtga tccgcgcggc
      661 gtgcgaaagc ggcaaacgca ttcgcgtggt ggcggacgag acccggccca tcctccaggg
      721 cgcgcgcctc accgcgtggg aactgatgca ggacaacatc ccggtgacgc tgatcaccga
      781 caacatggcg ggcgcgttga tgcggcaagg ccgcatccag gcctgcgtgg tgggcgcgga
      841 ccgcatcacc gccaacggcg acgtggcgaa caagatcggc acctacggcg tggccgtgct
      901 cgccaaacac cacggcctgc ccttctacgt ggcggctccg acctccacgc tcgatctgtc
      961 gctacgatcg ggcgcggaca tcccgatcga atcccgcaac ccgcgcgaag tcacgcacgt
     1021 caacggccaa gcgcatatcg cgccggacgg cgtggccgtg gacaaccccg cattcgacgt
     1081 gaccccggcg gagttgatca cggccatcat cacggaaaag ggcgcgttcc gtccggccga
     1141 catccgccgg ctggacggcg accccgagtc gctcgccgcg caccgcctcg cgtaacgcga
     1201 ggccccgcca acggaacctg ccgtcatcgc ttcgccatgc gtatggtgtc gaagagtcat
     1261 ttgtcgcgcc gccgggaatc cgcgcgctcg tcgtgttgtt cgacgtggtt aaaacgtgta
     1321 gagcagcgac gtcttgaaca cgcgatcgta cctggtgaat cccgccggcg gatcgctgtc
     1381 gtataggacc gcgtaggaca ccttccacgc gaaattgccc atcaggttcg tgatgaacga
     1441 ggattcctcc ttggccagaa agcccacgcc gcctttcaag ccgggaaggt attccgcggt
     1501 ctggagaaac cgggtcttgt catcgaacgc gtgattgtac tcgccgtagg ctcgtccggt
     1561 catgtaccca aggtcgtgaa gcgggtcgat cgggggcgtc gtgtcggtgg gatcgagcgc
     1621 aatggggttc tcgcggatgt aacccacgcc caattcgccc ttgagcagat ccgacggcgt
     1681 tttgatgaac tcgtatccca taccggcctg agtcgtgtac cgtatctcaa cgcccttgag
     1741 cacgttgcgt tccaccgttt ccgcgagata cgcgaaggcg cgactgctga caaaccgatc
     1801 gtacttgaga cgccccatcc agttcttgtc cgacgcgacc gtttcttttt ccccatagat
     1861 ggcgaggaac tccccggtga gcttggcgcg atccaacgcc cgctcggcct tgcccccggc
     1921 cgcgagcgtc gtggagctgg tgttgccgct ggtttgcagg tacgacaact ccaggttgcc
     1981 tttccacaag ccttcctctt gggcccaaga cggcgacgcg cccaagagca cgacgccggc
     2041 cagaccacat cccgtcatcc agtcacgcat ccgtttcatc agcatctcct gtctggtgac
     2101 gatctatcgc gtcggaatca ggcagcgacc gacgcggcta cgcggccttg atttccgacg
     2161 tgcactgcgg gcagcggctt gccttgaggg gaatggtcga cacgcaatac gggcacgcct
     2221 tggtcgtggg cgcggccgcc ggcggctcgg gctggcgttg cagtcttgcg aactgcttga
     2281 ccaggacgaa gatcacggcc gccacgatga gaaagtccac caccgtgttc aggaacatgc
     2341 cgtaattgag ggtcggcgct ccggcggcct tggccgcggc caggcttgga tacgcggtgt
     2401 cagacaggtt gataaagaga tcggcgaaat tgacgcgacc ggcgatgagc ccgatcggcg
     2461 gcatcaacac gtcgctgacc agcgaggtga cgatttttcc gaacgcggcg ccgatgatga
     2521 tgccgaccgc catgtccagg acgttcccgc gcaaaatgaa tgctttgaag tctttgagca
     2581 tgatcccctc cctgggttgg aagtcgcgct cattcgccgg tgagaaccgg cgggcggcgt
     2641 tgagaaccga tcccttacag gaaacgcggg agcagaatga cggcccagat cacccccgcg
     2701 ttgaacaggg cgagcaacac cacggccgat cccacgtcct tggctttttt cgagagcgcg
     2761 tggcggtccg ggccgatccg atccaccacc gcttcgatcg ccgtgttgac cagctccgcg
     2821 atcaacacca ggaacagact gccgatgagc agcgcgcgct cgacgccgct gcgccccacc
     2881 cacaggccga gcggcgcggc cacgaggaag accgccacgt ccgcgcgaaa cgcccactcg
     2941 ctccgccacg cgg
//
LOCUS       DBNGTT010000434         4683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3041569, whole genome shotgun sequence.
ACCESSION   DBNGTT010000434 DBNGTT010000000
VERSION     DBNGTT010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3041569"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="miaA"
                     /locus_tag="AC16BC_09775"
     CDS             <1..864
                     /gene="miaA"
                     /locus_tag="AC16BC_09775"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR3241949.1"
                     /translation="IVCADSRQVYRGMEIGTAKPTAAERARVPHHLIDLVDPDEAYSA
                     GRYARDAAACLEALRARGARALVVGGTGLYVRALLWGLCDGPTADPALRESWLRREQN
                     ELGVVYRRLSEVDPASAAAIHPNDLAKALRAVEVFELTGTPLSSRQRDHGFLAPQVDA
                     VVIGLRRERADLYGRIDSRVDAMTAQGLVAEVEGLTRRGYGDDAPGMRAVGYRQLAGA
                     LKGAYGLDEAIRLVKRDSRRYAKRQMTWFGADSSTHWVDASSDTPVEELVDRVTTCLE
                     GVVCGGENRRG"
     gene            864..1751
                     /gene="mtnP"
                     /locus_tag="AC16BC_09780"
     CDS             864..1751
                     /gene="mtnP"
                     /locus_tag="AC16BC_09780"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HIR3241950.1"
                     /translation="MARTKTARNAPRIGIMGGSGLYEMEGFKAQRRERVRTPFGAPSD
                     AVTIGSLNGVEVAFLARHGAGHRHSPTRIPYRANIHAMKQLGVERILSVSAVGSMKET
                     IHPGEMLIPDQFYDHTKHRVSTFFDNGIVAHVAFGDPVCPELTAALAAGCGGLGLTAH
                     ARGTYLCMEGPQFSSRGESLIYRQWGVDVIGMTNATEAKLAREAEICYATLALVTDYD
                     CWHQEESDVTVEAVIKLLHENVAKAKRVLAAVVPTIAAARACRCGDALRYAIITRPEA
                     VSASAKARLKLLLGGWGKR"
     gene            1748..2470
                     /locus_tag="AC16BC_09785"
     CDS             1748..2470
                     /locus_tag="AC16BC_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241951.1"
                     /translation="MTRRGSLGFVLAIWAAAGCAAPLGPSLSHDETQLFRADYFDWPR
                     LKSGGLGLLGVTSTVAPEGIREDVAFVLDQAASNYLTRLRLVPHGDAAARAREVGMGA
                     DLDRLLRNYEVRGAIDAGVLRRIASAEGVRYFLVARIARYERTTREMEGPPTVAVTPG
                     MIPPKRTAERVQRIRLLGEVWDSRCGNVVWSGEGGTEAVEEAVSEDVRLQDVLSMAAT
                     NLVSLLPRPGDASKAVEKECGA"
     gene            2467..3381
                     /locus_tag="AC16BC_09790"
     CDS             2467..3381
                     /locus_tag="AC16BC_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3241952.1"
                     /translation="MSLLVVGSVALDSVKTPFGEATEVLGGSATYFSTAASYFTPVNV
                     VATVGEDFPAAHLEFLRSRGVDIAGIETGNGKTFRWRGEYSYQLNEAHTLETQLNVFE
                     RFAPRVPEAYRKPALVFLANIDPVLQRDVLGQVTRPKIVACDTMNFWIAGKKGELERT
                     LREVDILIINDGEARQLAGEVNLVKASRAIMQMGPRTVIIKRGEHGALLFSGAKVFAA
                     PAFPLENVFDPTGAGDSFAGGFLGHLAKHPSLDHTNLCKAVIFGSVLASYCVEAFSLD
                     RFRTLTRLEIQHRYREFKHLTFFEDVDA"
     gene            3449..4180
                     /locus_tag="AC16BC_09795"
     CDS             3449..4180
                     /locus_tag="AC16BC_09795"
                     /inference="COORDINATES: protein motif:HMM:NF051128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3241953.1"
                     /translation="MWAGLSAILLLTACANQADIRQADAHYKMGYSYLIDQQIQPAFV
                     EFQKAVELNPKDRDSHYALGHVYFMKEQLDDAEREFRRVIKLKADDAAAWNYLGKVYD
                     EQGKPEQALQAYDRALSFPQYATPDLAHYNKGKVYAKRGQPTDALREFNMALRINPTH
                     ALAAFEAGRLHEQDPNGVTQAIAAFEAAVKNAPTFAEAYYHLAKTYTRAGKTEQARDA
                     YRKVVELAPGSRWAEDANAQIARPQ"
     gene            4207..>4683
                     /locus_tag="AC16BC_09800"
     CDS             4207..>4683
                     /locus_tag="AC16BC_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3241954.1"
                     /translation="MESFGQYLRGQRGTRGVSLEEVSVRTRIRLQTLEALERDEYTAL
                     PAEVTVKGFLRSYARSLGLDERDVLARYQQYAAEYFQIAEESDPVKRVLPPDRHRLWQ
                     HHLTIGGLAAAGVMVVVSVIALWSPRRPPEHPTAISSPLEMPPPIIDGQGHPSMVPL"
BASE COUNT          815 a         1373 c         1690 g          805 t
ORIGIN      
        1 atcgtgtgcg cggattcgcg gcaggtgtac cgggggatgg agatcgggac cgccaaaccc
       61 acggccgcgg agcgcgcgcg ggtgccgcac cacctgatcg atctggtcga cccggacgaa
      121 gcgtacagcg cggggcggta tgcgcgcgac gcggccgcgt gtctggaggc gttgcgcgcg
      181 cgcggcgcgc gggccctcgt ggtcggcggg accgggctct atgtgcgcgc gttgttgtgg
      241 gggttgtgtg atggaccgac cgctgatcca gcgctgcgtg aatcctggtt gaggcgcgag
      301 cagaatgagt tgggtgtcgt ataccggcgg ctcagtgagg tcgatcccgc ttcggcggcc
      361 gcgatccatc ccaatgacct ggcgaaggcg ctgcgggcgg tcgaagtgtt cgaactgacc
      421 ggcacgccgt tgtcgagccg acagcgggat cacgggtttc tcgccccgca agtcgacgcg
      481 gtggtgattg ggcttcgccg cgaacgggcc gacctgtatg gacgcatcga ctcccgcgtg
      541 gacgccatga cggctcaagg gcttgtagcg gaggtcgagg ggctgacgcg tcgcggctac
      601 ggcgacgacg cgccgggcat gcgcgcggtg ggctaccgtc aactcgcggg cgccctgaaa
      661 ggcgcgtacg ggctggacga agcgatccgg ttggtcaagc gggatagccg ccggtacgcg
      721 aaacggcaaa tgacctggtt cggtgctgat tcgtccaccc actgggtgga tgcgtcgtcg
      781 gacaccccgg tggaagaact cgtggaccgc gtcacgacgt gtctggaggg cgtcgtgtgc
      841 gggggagaga atcggagagg atgatggcac gcacgaagac ggcgcggaac gcgccgcgga
      901 tcgggatcat gggcggcagc ggattgtacg agatggaggg cttcaaggcc caacgtcgcg
      961 agcgtgtgcg aacgccgttc ggggcgccgt cggacgccgt caccatcggc tcgctcaacg
     1021 gggtggaagt ggcgttcctg gctcggcacg gcgccggaca ccggcactcc ccgacccgca
     1081 ttccgtaccg cgcgaatatt cacgccatga agcaactcgg ggtcgaacgg atcctctcgg
     1141 tcagcgcggt gggcagcatg aaggaaacga tccatcccgg cgagatgttg atcccggatc
     1201 agttctacga ccacaccaaa caccgcgttt cgacgttctt cgataacggg atcgtggcgc
     1261 acgtcgcgtt cggggacccg gtctgtcccg agctgaccgc ggctttggcg gccgggtgcg
     1321 gcggtctagg gttgaccgcg cacgcgcgcg gaacatacct gtgcatggag ggaccgcagt
     1381 tctccagccg gggcgaatcg cttatctacc ggcagtgggg tgtggacgtg atcggcatga
     1441 ccaacgccac cgaggccaaa ctcgcgcggg aggccgaaat ctgctacgcc acgttggcgc
     1501 tcgtgaccga ctacgattgt tggcatcagg aagagtcgga cgtgaccgtc gaggcggtga
     1561 tcaaactcct gcacgagaac gtagccaaag ccaaacgggt gctggcggcc gtggtgccga
     1621 cgatcgcggc ggcgcgcgcg tgccggtgcg gggacgcgtt gcggtacgcg atcatcacca
     1681 ggcccgaggc ggtgtccgcg agcgccaagg cccgcctcaa actcctgctc ggtggatggg
     1741 gaaagcgatg acgcgtcgcg gctcgctcgg cttcgtcctg gcgatctggg ccgcggccgg
     1801 atgcgctgcg cccttgggac cgtcattgtc tcacgatgag acgcaactgt ttcgcgcgga
     1861 ctactttgac tggccccgac tcaagtcggg cgggctgggc ctgctcgggg tgacgtcaac
     1921 cgttgcgccg gaagggattc gggaggatgt cgcgttcgtc ttggaccaag ccgcgtccaa
     1981 ctacctgacg agactccggc tggtcccgca cggtgacgcg gccgctcgcg cccgggaagt
     2041 cgggatgggc gctgacctgg atcgtctgct gcgcaactac gaggtgcggg gcgcgatcga
     2101 cgcgggcgtg cttcggcgca tcgcctcggc cgaaggggtg cggtattttc tggtggcccg
     2161 catcgcgcga tacgagcgca cgacgcggga gatggaaggg ccgccgaccg tggcggtcac
     2221 gccggggatg atcccgccga agcggaccgc cgaacgcgtc cagcggatcc ggctgctcgg
     2281 ggaggtttgg gacagtcggt gcggcaacgt ggtgtggagc ggagaaggcg ggaccgaggc
     2341 ggtcgaagag gcggtgtcgg aggacgtccg cctgcaggac gtcttatcca tggctgcgac
     2401 gaatctggtg agccttttgc cgcgtccggg cgacgcgtcg aaggccgttg agaaggagtg
     2461 tggcgcatga gtttgttggt ggtggggtcg gtggcgttgg attcggtcaa gacgccgttt
     2521 ggtgaggcga ccgaggtgct gggggggtcg gccacgtact tttcaaccgc ggcgtcgtat
     2581 tttacgccgg tgaacgtggt ggcgacggtc ggcgaggatt tcccggccgc gcatttggag
     2641 tttctgcgca gtcggggagt cgacatcgcc ggcatcgaga ccgggaacgg gaagacgttt
     2701 cggtggcgcg gcgaatacag ttatcaactc aacgaggctc acacgttgga gacccagctc
     2761 aacgtgttcg agcggttcgc cccgcgggtg ccggaggcgt atcggaagcc tgcgctcgtg
     2821 ttcctggcca acatcgaccc cgtgttgcag cgcgacgtgc tcgggcaggt gacccgtccg
     2881 aagatcgtgg cctgcgacac catgaacttc tggatcgccg ggaaaaaggg cgagttggag
     2941 cgcaccctgc gggaggtgga tatcctgatc atcaacgacg gcgaggcccg gcagttggcg
     3001 ggtgaggtca acctcgtgaa ggcgagccgg gcgatcatgc agatggggcc gcgtaccgtg
     3061 atcatcaaac gcggggagca cggcgcgttg ctgttctccg gggccaaggt gtttgccgcc
     3121 cctgcgttcc cgctggaaaa cgtctttgat ccgaccggcg cgggcgacag ttttgccggc
     3181 ggtttcctcg gccacctcgc caaacatccg tcgctggacc acacgaattt atgcaaggcg
     3241 gtgattttcg gcagcgtgtt ggcgtcgtac tgcgtggagg cgttcagtct ggaccgcttc
     3301 cgtacactca cgaggttgga gattcagcac cggtatcggg agttcaagca tctcaccttt
     3361 ttcgaggacg ttgacgcgta aggaggcggc tgtggaacga acatgcaccc ctcgagggtg
     3421 gtccgcaagc cggccgggag ggtcggtggt gtgggccgga ttgtctgcta tactcctcct
     3481 cactgcctgc gcgaaccagg ctgacattcg gcaagcggat gctcactata agatggggta
     3541 ttcgtacttg attgaccagc agatccaacc cgcgttcgtg gaattccaga aggccgtcga
     3601 actcaatccc aaggatcggg acagccacta cgcgttgggt cacgtttact ttatgaaaga
     3661 gcagctcgat gacgcggagc gtgagttccg gcgcgtcatc aaactgaagg cggacgacgc
     3721 agcggcgtgg aactatctcg gcaaggtgta cgatgagcag ggaaagcctg aacaggcgct
     3781 gcaggcgtac gatcgcgcgt tgagtttccc gcaatatgcc acgccggacc tcgcgcacta
     3841 caacaagggc aaggtgtacg ccaaacgcgg gcagccgacc gacgcgttgc gcgagttcaa
     3901 tatggcgttg cgcatcaatc cgacgcacgc actcgccgcg ttcgaggcgg gccggctcca
     3961 tgaacaagac ccgaacggcg tgacgcaggc gatcgcggcg tttgaggccg cggtcaaaaa
     4021 cgcgccgacc ttcgccgagg cctattacca cttggccaag acctatacgc gcgcggggaa
     4081 gaccgagcag gcccgtgacg cctaccggaa agtcgtcgag ttggcgcccg gcagccgttg
     4141 ggcggaggac gcgaacgcgc agatcgcgcg gccgcaataa cgcagggcgc aaggagtcgt
     4201 tatcgcatgg aaagtttcgg acaataccta cgtggacagc gcggcacgcg gggcgtctca
     4261 ttggaggagg tgtccgttcg cacgcgaatc cgcctgcaaa cattggaggc gttggagcgg
     4321 gacgagtaca ccgcgttgcc ggccgaagtg acggtcaaag ggttcttgcg gtcctacgcg
     4381 cggtctctgg ggctcgacga gcgcgacgtg ctcgcgcggt atcaacagta cgccgccgag
     4441 tacttccaga tcgcggagga aagcgacccg gtcaagcgcg tcctgccgcc ggaccggcat
     4501 cggttgtggc aacaccacct cacgatcggc gggctcgccg cggcgggggt gatggtggtg
     4561 gtgtccgtca tcgcgctgtg gagtccgcgc agaccccctg agcaccccac cgcgatttcg
     4621 tcgccattgg aaatgcctcc accgatcatc gatggccagg gccatccctc gatggtgccg
     4681 ttg
//
LOCUS       DBNGTT010000435         4538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2912878, whole genome shotgun sequence.
ACCESSION   DBNGTT010000435 DBNGTT010000000
VERSION     DBNGTT010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2912878"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..353)
                     /locus_tag="AC16BC_09805"
     CDS             complement(54..353)
                     /locus_tag="AC16BC_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241955.1"
                     /translation="MPALAAEPKAPAKPPMMAEETVTITEVNYFSNFNIAAYSITAGT
                     MEIRADKQGPPCPGTEAGTKIAARYDNKLMFLGPEKDRCKLTIRRMEFTGPPQPH"
     gene            483..857
                     /gene="queF"
                     /locus_tag="AC16BC_09810"
     CDS             483..857
                     /gene="queF"
                     /locus_tag="AC16BC_09810"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR3241956.1"
                     /translation="MPTSPSKSLDTFHNPNPDRDYEIRFECPEFTCLCPKTGQPDFAT
                     IRIRYIPDRLCVELKSLKLYLWSYRNEGAFHEAVTNAILNDLVSTIQPRFAEIEGDFF
                     VRGGIHTVVTAKHRAPGFTPQP"
     gene            854..1321
                     /locus_tag="AC16BC_09815"
     CDS             854..1321
                     /locus_tag="AC16BC_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HIR3241957.1"
                     /translation="MSVWARLHITAFAFWVGVMAFLVTLAPITFQTFPQAQAGDLMSK
                     LFVPYHVVGYVCAGTALLTQWLAAGFRGKVRNILLIAMLGATLYAGLVISPEARALRT
                     ELRQADAATENPATKARFDTLHRRSVTVNSGILIAALTALVWAVVSPPHRKKS"
     gene            1354..2166
                     /locus_tag="AC16BC_09820"
     CDS             1354..2166
                     /locus_tag="AC16BC_09820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3241958.1"
                     /translation="MTTLGIQPQNAKMAATWNTGGQQYERISQTIADSIEHCVTRLNP
                     RPGERVLDVATGTGWTARRAAMRGAIVAGVDLGEDLIAAAKAAAAEARLTIDFRVGDA
                     EKLPFADQCFDAVVSTCGVMFVRDPEAAARELARVCKKGGRLGLTTWPADGTVAGLFK
                     VMKPYMAAPPTPPPPSPFEWGNPDRVRQLLGSAFDLKFETGTTVLREPSGQAVWDIFV
                     AGYGPTKSLAANLDPERREKLKQDFIAFHDGFRTELGVAMPRDYLVTIGVRK"
     gene            2175..2426
                     /locus_tag="AC16BC_09825"
     CDS             2175..2426
                     /locus_tag="AC16BC_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3241959.1"
                     /translation="MGSGGAAIAFDRMDSFNTFNSAAYILLMFLSTMFYPIDSLPAWF
                     RAIAWINPMTWQVDVLRYSLPGIGSATVVLITPNQRGQT"
     gene            2471..3400
                     /locus_tag="AC16BC_09830"
     CDS             2471..3400
                     /locus_tag="AC16BC_09830"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR3241960.1"
                     /translation="MARKPRIEYPGALYHVIARGNQRRDVFHDDMDRLRYLARLRAYK
                     ERYGFRLYAYALMTNHVHLLLEPQQTGLSKIMQGLHQSYTQGYNRRYCAVGHVFQGRY
                     QAILCDRDAYLLALVRYIHLNPVRARLVRDPADYPWSGHRAYLDSVGEGLVDTETVLG
                     MLGERRSLAQKRYQAFVTEELTGGSRPEYYQVRDQRFLGDDAFVEQMQRTTAEERRQP
                     VKTPSLAALLSAVVRATGVTRERITGPDRRGDAARARRLLVQAGAICAVPGRDLARLL
                     GRDPAVISRLARMNTNERSAVTRLVAGKSTSQV"
     gene            3610..3675
                     /locus_tag="AC16BC_09835"
     CDS             3610..3675
                     /locus_tag="AC16BC_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR3241961.1"
                     /translation="MGRNDPCPCGSARRFQELLYA"
     gene            3767..4456
                     /locus_tag="AC16BC_09840"
     CDS             3767..4456
                     /locus_tag="AC16BC_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241962.1"
                     /translation="MAFLILCSGCASYVTPGGPVRLADINRADILEVASRKPSPNFPA
                     RIAIARVQAPEYKSYSTEGFGTGRFSVLTTQELLTEEQLDSIGKWPSVAGVTTVNRLL
                     LPSRLESLDDLRLAAAKVQADVLLVYTIDTTFRVQGRTYGPLSVISLGLAPDRDAYIT
                     STASALFTDVRTGFVYGTAESTAKASDLTSFWGSKNTIDKKRLEAEQHAFNAFVPEAE
                     KTWAGIAKQYQ"
BASE COUNT          825 a         1403 c         1435 g          875 t
ORIGIN      
        1 gggaacgacg gattgaggca gggccccgca actgagcgga gtctgcgtcg cggtcagtgc
       61 ggttgcggcg gacccgtgaa ctccatccgc cggatggtga gcttgcagcg gtccttttcg
      121 gggccaagaa acatcaattt gttgtcgtag cgcgccgcga tcttggtccc ggcctcggtg
      181 cccggacacg gcggcccctg cttgtccgcc cggatctcca tggtcccggc cgtgatcgaa
      241 tacgccgcga tgttgaaatt cgaaaagtag ttcacctcgg tgatggtgac cgtctcctcc
      301 gccatcatgg gaggtttcgc aggagccttg ggctcggctg ccagggcggg catgccggac
      361 agcaggagcc acaaacccgc gcctattggt cctattcgtc tcataggtcc tattgctttc
      421 gcgattctac gcaagcccgg cccggattgc aaagcgcggc agccctacgg tacgatcctg
      481 ccgtgccgac cagtccgtcc aaatccctcg acacgttcca caacccgaat cccgatcgcg
      541 attacgagat ccggtttgag tgcccggagt tcacctgttt gtgtcccaaa accggccagc
      601 ccgacttcgc gacgattcgc attcgctaca tcccggatcg gctgtgcgtc gaactcaagt
      661 cgctgaagct gtatttatgg tcttatcgca atgaaggcgc gttccacgaa gccgtgacca
      721 acgccatcct caacgacctg gtgtccacga tccagccgcg ttttgccgag atcgaaggcg
      781 acttcttcgt gcgcggcggc atccacaccg tggtcacggc caaacaccgc gccccgggat
      841 tcaccccgca gccatgagcg tctgggcgcg actgcacatc acggcgttcg cgttctgggt
      901 gggcgtgatg gcgttcctgg tcaccctggc gccgatcacg tttcagacgt ttccgcaggc
      961 gcaggcgggc gatttgatga gcaagctgtt cgtgccctat cacgtggtcg gctacgtgtg
     1021 cgccggcacg gcgctgctga cccagtggct ggcggcgggg tttcgcggca aggtccgcaa
     1081 catcctgctg atcgccatgc tgggcgccac gctctacgcc gggctggtca tctcgccgga
     1141 ggcgcgcgcc cttcggacgg aactccgcca ggccgacgcc gcgaccgaga accccgcgac
     1201 caaagcgcgg ttcgataccc tgcaccgtcg ctccgtcacc gtaaacagcg gcattttgat
     1261 cgcggcgctc acagcgctcg tttgggcggt ggtctcgccg ccgcaccgca aaaagagctg
     1321 agcatcccgt cacgggtgct cgaaggagga gctatgacca cgctgggcat tcaaccccag
     1381 aacgcgaaaa tggccgccac ctggaacacg ggcgggcagc agtacgagcg catcagccag
     1441 accatcgccg actccattga gcactgcgtc acgcgcctga atccccggcc cggcgagcgc
     1501 gtgctggacg tggccacggg cacgggctgg accgcgcgcc gcgcggctat gcgaggcgcc
     1561 attgtggccg gggtggatct gggcgaggac ctgatcgcgg cggccaaggc cgcggcggcc
     1621 gaggcccggc tgaccatcga ctttcgcgtg ggcgacgccg agaagctgcc gttcgcggat
     1681 cagtgcttcg atgcggtcgt ctcaacctgc ggggtcatgt tcgttcgaga tcccgaggcg
     1741 gccgccaggg agttggcgcg cgtgtgcaag aaaggcggcc ggctcgggct caccacctgg
     1801 cccgcggacg ggaccgtcgc gggcctcttc aaagtcatga aaccctacat ggcggcgccg
     1861 cctacgcctc cgccgccgtc gccgttcgag tggggcaacc cggatcgcgt gaggcaactc
     1921 ttagggtcgg cctttgacct gaaattcgaa accggcacca cggtgctgcg cgagcccagc
     1981 ggtcaggcgg tctgggacat cttcgtggcc ggctacggcc ccaccaaatc attggccgcg
     2041 aacctggacc cggaacgccg cgagaaattg aagcaggact ttattgcctt ccacgacggc
     2101 ttcaggaccg aactcggcgt ggccatgccg cgcgactatc tggtgacgat cggcgtccgg
     2161 aagtagcggc gacgttgggt tcgggggggg cagcgatcgc tttcgaccgc atggactcct
     2221 tcaacacctt caactccgcc gcctacatcc tcctgatgtt cctcagcacc atgttctacc
     2281 ccatcgattc cctgcccgcg tggttccgcg ccatcgcgtg gatcaacccc atgacctggc
     2341 aggtggacgt gctgcgctac agcctgccgg ggatcgggtc ggcgaccgtc gtgctcatca
     2401 ctcccaacca aaggggtcag acttgactta ttgactttgg tgggcgcctc ctgctacggt
     2461 gcgccgcatc atggcccgta agccccgtat cgagtatccc ggagcgctct accacgtgat
     2521 tgcgcgcggc aaccagcgcc gagacgtgtt ccacgacgac atggaccgac tgcgctatct
     2581 cgccaggctg cgggcctaca aagaacgcta cggcttccgg ctgtatgcgt acgccctgat
     2641 gactaaccac gtccacctgc tgcttgaacc gcagcagacc gggctgtcga aaatcatgca
     2701 gggcttgcat cagagttaca cccagggcta taaccggcgc tattgcgcgg tcgggcatgt
     2761 ctttcaaggg cgctatcagg cgatcctgtg tgatcgggac gcgtacctgc ttgcgctggt
     2821 tcggtacatt catctcaatc ccgtgcgcgc gcggcttgtc cgggatcctg ccgactatcc
     2881 ctggagcggg catcgggcgt atctggactc ggtgggtgag gggctcgtcg atacggagac
     2941 ggtgctggga atgctgggcg agcgccgatc gttggcccag aagcgctacc aggcctttgt
     3001 aactgaggag ctgaccggtg ggagtcgacc agagtattat caggtgcgcg atcagcgctt
     3061 tctgggtgac gacgcgttcg tcgaacagat gcagaggact acggccgaag agcggcgcca
     3121 gccagtgaag acgccgtcgt tggcggcgct gctcagtgcg gtggtgcggg ccaccggtgt
     3181 tacgcgcgag cgcattaccg gacctgatcg ccggggcgat gccgcccggg cacggcggct
     3241 actggtgcag gcgggtgcga tctgcgcggt gcctggtcga gacctggccc ggctgctggg
     3301 gcgggatccc gccgtgatct cgcggcttgc gcggatgaac accaacgagc gatcagcggt
     3361 cacgcggttg gtggcgggaa agtcaacaag tcaagtctga ccccgagggg gcgtagaaat
     3421 gagcgtcgga ttcaggtagt ctttcgtctc gaaaactccc atccaggagg tgccacatgg
     3481 caaagaataa tttcgacgac gctccctgct ttcgagccca cgagtaagcg gcgtaaaggg
     3541 ttcccgtccg aaacgcgcgt caagcgcggg caacagatcg ttcacggcaa caaagaactg
     3601 cacgaaaagc tggggcggaa cgacccgtgc ccctgcggat cagcccgccg gtttcaagaa
     3661 ttgttgtatg cgtagcggcc ggtacgacgg cagtccgcgc gactactttt tttagagaat
     3721 gattcccgta gatttgctga ggaggatcat catgagatag ttactcgtgg cttttctgat
     3781 tctctgttcg ggttgcgcca gctacgtgac gcccggtggt ccggtgcgcc ttgctgacat
     3841 taatcgtgcc gatattttgg aggtggcatc acgtaagccg tcgccgaact ttccagcccg
     3901 catcgcgatc gcaagagttc aggcgccgga gtacaagtcg tactcgactg aggggtttgg
     3961 gaccgggcgg tttagtgttc tgacgacgca ggaactcctg acggaagaac agttggacag
     4021 catcggcaaa tggccctctg tggctggcgt gacgacggtc aatcgccttc tcctgccgtc
     4081 aagactggag tccctggacg atcttcgcct tgcagcggca aaggttcagg cagacgttct
     4141 gctggtctat acgatcgaca ccacattccg agtccaaggc cgcacatacg ggccactatc
     4201 ggtcatttcg ctcggtttgg ctcctgatcg agatgcctat attacatcca cggcttcagc
     4261 gctcttcacc gatgtccgca ctggcttcgt ctatggcact gcagaatcga ccgctaaggc
     4321 ttccgacctc acgagttttt ggggctcgaa gaatacgatc gataagaaac gtcttgaagc
     4381 cgagcaacac gcattcaatg cttttgttcc ggaggctgag aagacgtggg cggggattgc
     4441 gaaacagtat cagtaagcgg tcagcgaggt tgatgaggct tctatggaac gcagagacga
     4501 gtaccgcacg ctcggacgca ccgggctcag ggtgtctc
//
LOCUS       DBNGTT010000436         1561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3423956, whole genome shotgun sequence.
ACCESSION   DBNGTT010000436 DBNGTT010000000
VERSION     DBNGTT010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3423956"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="AC16BC_09845"
     CDS             complement(<1..372)
                     /locus_tag="AC16BC_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241963.1"
                     /translation="MAADGEDHAVSLAEILRQTRAHVRFARELGVTDVPKPRLQETGR
                     PPASRGTLDTRRMRRAPEPPPSDMTPRSPTPQPVTGNPADALRCLRTETIGDCQRCGL
                     APTRTNLVFGVGNPQAEVVFVG"
     gene            complement(362..865)
                     /gene="tsaE"
                     /locus_tag="AC16BC_09850"
     CDS             complement(362..865)
                     /gene="tsaE"
                     /locus_tag="AC16BC_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR3241964.1"
                     /translation="MSRAAISEPDTVWRCLSSSEAETRALGRRLGERLAGGEVLSLIG
                     ELGAGKTRFVQGLAEGLGVEPRRVSSPTFAIRHDHAGRVALLHLDFYRLHDPEEVEWL
                     GMLEAPDDAVIAVEWADRLPDALPDDRLEIAFTPGPTPDDRSLTWTAYGPRAERALAA
                     LRAVHGR"
     gene            complement(862..>1561)
                     /locus_tag="AC16BC_09855"
     CDS             complement(862..>1561)
                     /locus_tag="AC16BC_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241965.1"
                     /translation="GRIGPDGSIDRAPARPPHQTFIPQHGKERHMTLFGITLAFAILL
                     AALGSAMIIHSIVGKRRILADFRTLSERIGGHTSQPTVFSFPRVDGRYDGREASVFFH
                     LAKGEKASIIYVVYTLSAKAATALFLSKDKYYKTVNDPTKLSREAGEVVPGLDGRYEV
                     RTSDAARASRLFNQGDIRTHLDALEPFPSLVLGPDLLVAGKPFESPADTHPESALKAF
                     RALDKIAAEMEAVA"
BASE COUNT          245 a          513 c          545 g          258 t
ORIGIN      
        1 cccgacgaac accacctcgg cttgcggatt acccaccccg aagaccaggt tcgtgcgcgt
       61 gggcgcgagc ccgcagcgtt ggcaatcgcc gatggtctct gttctcaaac accgcagcgc
      121 gtccgccgga ttcccggtga ccggttgcgg cgtcggcgag cgcggcgtca tgtccgacgg
      181 cggcggttcg ggcgcgcgtc gcatccggcg cgtgtcgagg gttcctcggg acgcgggcgg
      241 gcggcctgtc tcctggaggc ggggcttggg cacgtccgtg acgcccagtt cccgcgcgaa
      301 acgcacgtgc gccctggtct ggcggagaat ctcggcgagg gacacggcgt ggtcctcgcc
      361 gtcagcggcc atggacggcg cgcagcgcgg ccaacgcgcg ttctgcgcgc ggaccgtatg
      421 cggtccaggt aagcgaccgg tcgtccgggg tcggtcccgg cgtgaacgcg atctccagac
      481 gatcgtcggg taacgcatcc ggtaggcggt cggcccactc gacggcaatc accgcgtcat
      541 caggcgcttc cagcatgccc aaccactcga cctcctcggg atcgtgcagc cggtagaagt
      601 ccaggtgcag gagggcgacc cttccggcgt gatcgtgacg gatcgcgaag gtcggactcg
      661 acacccggcg cggttcgacg ccgagccctt ccgccaatcc ttgaacaaag cgggttttgc
      721 cagcgcccaa ctccccgatc agcgagagga cctcgccgcc cgccagtcgc tctccgagac
      781 ggcggcccag ggcgcgggtc tccgcctcgg acgacgagag gcagcgccac accgtgtccg
      841 gctcgctgat tgcggcgcgg ctcacgcgac ggcttccatc tccgcggcga tcttgtcgag
      901 cgcgcggaac gctttgagcg ctgattccgg gtgggtatcc gccgggctct cgaacggttt
      961 gccggccacc aggaggtccg ggccgagcac caggcttgga aacggctcca acgcgtccaa
     1021 atgggtgcgg atgtcgcctt ggttgaacag tcggctggcc cgcgccgcgt cgctggtccg
     1081 cacttcatag cggccgtcaa gacccggcac gacctcgccc gcttcgcggg acagcttggt
     1141 cggatcgttc acggtcttgt agtacttgtc cttggagagg aacaaggcgg tcgcggcttt
     1201 ggccgagagc gtgtagacca cgtaaatgat gctggccttc tcgcccttgg cgaggtggaa
     1261 gaagaccgag gcctcccggc cgtcgtaccg gccgtcgacc cgcgggaacg aaaagaccgt
     1321 gggttgcgac gtatgcccgc cgatgcgttc cgagagcgtc cgaaagtcgg ctagtatccg
     1381 tcgcttcccc acgatggagt ggatgatcat ggccgagccg agcgcggcga gcaggatggc
     1441 aaatgccaaa gtgatgccga acaacgtcat gtgtcgctcc ttgccgtgtt gagggatgaa
     1501 cgtttgatga ggcgggcggg ctggtgcgcg gtcgatggaa ccatccggcc caatgcggcc
     1561 g
//
LOCUS       DBNGTT010000437         4985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_486556, whole genome shotgun sequence.
ACCESSION   DBNGTT010000437 DBNGTT010000000
VERSION     DBNGTT010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_486556"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="tsaD"
                     /locus_tag="AC16BC_09860"
     CDS             <1..928
                     /gene="tsaD"
                     /locus_tag="AC16BC_09860"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3241966.1"
                     /translation="QTGVHSKFGGVVPELACRGHVEVIDRVVEHTLAKAGVTASAVDA
                     VAVTSGPGLAGALLVGVSFAKGLAYALDRPLIGINHLEGHIVAPVLSGQSIAYPAVAL
                     IVSGGHTHLYVVEGFGRYRLVGHTIDDAAGEAFDKAAYCLGLGFPGGPAIDRLAGSGD
                     PTRFRFPRPLTGRGGGSHVGSRGFDMSFSGLKTAFVQALRREGPGDAAYPDLAAGFQA
                     AVVDVLVDKTIAAAKAAAVSDIVIGGGVAANSRLRAAIAARAAEHGFRLVMPTPRHCT
                     DNAAMIAAAAFHRAPLEHGAAMALNADPSLAL"
     gene            1211..2005
                     /locus_tag="AC16BC_09865"
     CDS             1211..2005
                     /locus_tag="AC16BC_09865"
                     /inference="COORDINATES: protein motif:HMM:NF005437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3241967.1"
                     /translation="MNIFITGASSGIGEALAVRLASPGRTLGLAARRASELERVALRC
                     RERGAAVSTFPLDVRDAQAVHDAGRAFLAEAGRVDAVVANAGVSRRDPDDRTDSEQSR
                     EVIETNLLGVIHTFEPFMAEMERAGAGSLVAISSVAGFRGLPNAGAYSASKAAVNTWM
                     ESLRVRLRGRVHVMTVCPGFVDTPMTASNRFRMPWLLTADDAAARIERGMARRTRVLV
                     FPAPMRWLMTCLRLIPSAWYEPVASWALRRNSDLLKKSDADPDPRS"
     gene            2160..4004
                     /gene="typA"
                     /locus_tag="AC16BC_09870"
     CDS             2160..4004
                     /gene="typA"
                     /locus_tag="AC16BC_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR3241968.1"
                     /translation="MTSVREDVRNIAIIAHVDHGKTTLVDRMLQQGGAFAAHETMGER
                     VMDSNALERERGITILAKNTAVRYQPPAGGPPILINIVDTPGHADFSGEVERVLTMVD
                     GVLLVVDAVDGPMPQTRFVLKKALELRLQPVVVINKIDRADARPVDVVNEVFDLFVEL
                     NASDEQLEFPVVFASAKTGIASHSATEPGRDLMPLFETIMQRIPPPPGDDAAPLQLQI
                     TNLDYDPYVGRIGLGRVARGTIRVNGAVARVSGDGRVEPGKITKLFLFHGLRRSEAQE
                     ARAGDIVAVAGLESLTIGDTLTAPDRPEGLPPLSIGEPTISMAFMVNNSPLAGREGKF
                     VTSRHLRDRLLRELRTNVALRVEESGTTDEFLVSGRGELHLAILIENMRREGYEFAVS
                     RPQVIFKTIDGETCEPMETLLLDVPESSVGVVMEALGPRRAELENMVNGGDGWVHLAF
                     TIPARGLFGYRSEYLTATKGEGLINHAFAGYRPSKGDVPTRNRGVLVALEPGESVTYA
                     LHNIQERGALFLGPGEPIYEGMIIGEHSRPGDLIVNPCKKKHLTNIRSSGAEDALVLT
                     PPRRLSLEQAIEFIEDDELVEITPQSIRLRKRYLNGEDRKRRRNAASA"
     gene            4066..>4985
                     /gene="guaB"
                     /locus_tag="AC16BC_09875"
     CDS             4066..>4985
                     /gene="guaB"
                     /locus_tag="AC16BC_09875"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR3241969.1"
                     /translation="MLSGDLEEGLTFDDVALIPAHSTVLPKDADTGTRLTKRIRLHIP
                     IVSAAMDTVTEAPLAIALARIGGIGIMHRVLSPEKQAEEVAKVKKSESGIITDPITIS
                     PDATINETVLIMERHEISGIPVTEQRKLVGIVTRRDLQFEKRMDLRVADVMTKGDRLI
                     TTPEGTTLEAASAIMQRHRIEKLPIVNDRFELQGLITVKDLKMAIEYPNAAKDERGRL
                     RVGAAVGVGREAHQRVDALVAAGVDVVVVDTAHGHSQDVLEMVQAVKSKYPNVDVIAG
                     NIATAQATEDLIKAGADAVKVGIGPGSICTT"
BASE COUNT          840 a         1576 c         1759 g          810 t
ORIGIN      
        1 ccagaccggc gtgcactcca agttcggcgg ggtggtgccc gagctggcgt gccgcgggca
       61 cgtggaagtc atcgaccgcg tggtcgagca cacgctcgcc aaggccgggg tgaccgcgtc
      121 ggccgtggac gcggtggccg tgacctccgg ccccgggctc gcgggcgcgc ttctggtcgg
      181 ggtgtcgttc gccaagggcc tggcctacgc gctcgatcgc ccgctgatcg ggatcaatca
      241 cctggaggga cacatcgtgg cgccggtgtt gtccgggcaa tccatcgcct atcccgcggt
      301 ggcgttgatc gtgtccgggg ggcacaccca cttgtacgtg gtcgaagggt tcgggcgcta
      361 ccggttggtg gggcacacga tcgacgacgc ggcgggcgaa gcgttcgaca aggcggcgta
      421 ttgcctgggc ctgggatttc ccggcggccc ggccatcgac cggctcgcgg gaagcggcga
      481 tcccacgcgg tttcgatttc cgcggccctt gaccggacgg ggcgggggaa gccacgtggg
      541 gtcgcgcgga ttcgacatga gtttcagcgg gctcaaaacc gcgttcgtcc aggcgttgcg
      601 ccgcgaaggg ccgggggacg cggcctaccc ggatctggcg gcgggatttc aggcggccgt
      661 ggtggacgtg ttggtggaca agaccatcgc ggccgcgaaa gccgcggctg tcagcgacat
      721 cgtcatcggc ggcggggtgg cggccaactc gcgcttgcgc gccgcgatcg cggcgcgggc
      781 ggccgagcac ggattccgcc tggtgatgcc cacgccccgg cactgcacgg acaacgccgc
      841 catgatcgcg gccgccgcgt ttcatcgcgc gccgctcgag catggggcgg cgatggcgct
      901 gaacgcggac ccgtcgctgg cgctgtagga gcctgtccat gaacctgagt gcaccgaggg
      961 tgcaagtgtt aattggcccg cagggaccct acaggtggcg cagcaaaggc catctgcggc
     1021 gttgtcggtc ggccgtgcgt gctcacgtac aacccagtac gctccgcgcg caaggtcctc
     1081 cctccgcctt gcatctggcc ttccctgaac aggctcaact agggatactc ggaagactcc
     1141 gaataaaaag ggcgagggtg gttccccgtt gatcggacaa ccaaggacga agtatgacga
     1201 tcattccgtc atgaacatct tcatcaccgg cgcgtcgtcc gggatcggcg aggcgctcgc
     1261 ggtgcgtctc gcgtcgccgg ggcggacgct cgggttggcg gctaggcggg cgtccgagct
     1321 cgagcgggtc gcgctccgtt gtcgtgaacg aggcgccgcg gtgtcgacct ttccgctcga
     1381 tgttcgcgac gcgcaggccg tgcatgacgc ggggcgcgcg ttcctggccg aggccggacg
     1441 cgtggatgcg gtggtcgcga acgcgggcgt gagtcgccgt gaccccgatg atcgaacgga
     1501 ctccgagcaa tcgcgagaag tcattgagac caatctgctg ggggtcattc acaccttcga
     1561 gccgttcatg gccgagatgg aacgagcggg cgcgggctcg ttggtggcca tttccagcgt
     1621 ggccgggttt cgcggcctgc ccaacgcggg cgcgtactcc gcgtccaagg ccgcggtcaa
     1681 cacctggatg gagtcgctgc gggttcggct gcgcggccgg gtgcacgtga tgaccgtgtg
     1741 ccccgggttc gtggacacgc ccatgaccgc ttccaaccgg ttccgaatgc cgtggctgct
     1801 caccgcggac gacgcggcgg cgcggatcga gcgcggtatg gcccggcgca cgcgggtgtt
     1861 ggtgtttccc gcgcccatgc gatggctgat gacgtgcctt cgcctcatcc cgtctgcatg
     1921 gtacgaaccg gtcgcgtcct gggcgctccg gcgcaattcc gacctgctta agaaatctga
     1981 cgcggaccct gacccgcggt cgtgacgccc ggcatgcttt accttgacca cgttcccgac
     2041 ggtgtgatac ggtagacaca gtttgttcgg gtggtcatcg tgttgagccc cgggactgcg
     2101 tcttggggct ttttcgtatc tatatgcagg gagcaggggg acacggggtg aacggtcaga
     2161 ttacgagtgt tcgtgaagac gtcaggaata tcgccattat cgcgcacgtt gaccacggga
     2221 aaacgaccct ggtcgatcgc atgctgcagc agggcggcgc gttcgccgcg cacgaaacca
     2281 tgggcgaacg ggtcatggat tccaacgcgt tggagcggga gcgcggcatc accattctgg
     2341 ccaagaacac cgcggtgcgg taccaacccc cggccggcgg acccccgatc ttgatcaata
     2401 tcgtggacac gccgggacac gcggacttct ccggcgaggt cgagcgggtg ttgaccatgg
     2461 tggacggcgt gctgctggtg gtcgacgccg tggacgggcc catgccccag acccggttcg
     2521 tgttgaagaa ggcgctggaa ctgcggttgc agccggtggt cgtcatcaac aagatcgatc
     2581 gcgcggacgc gcggccggtg gacgtggtca acgaagtgtt cgatctgttc gtcgagctca
     2641 acgcgagcga cgagcaattg gagttccccg tggtgttcgc gtcggccaag accgggatcg
     2701 cgtcgcacag cgcgaccgaa ccgggccggg atctgatgcc gttgttcgag acgatcatgc
     2761 agcggattcc gcccccgccc ggcgacgacg cggcgccgtt gcaactgcag atcaccaacc
     2821 tggactacga tccctacgtg ggccggatcg ggctcgggcg cgtcgcgcgc ggcaccatcc
     2881 gcgtgaacgg cgcggtggcg cgcgtgagcg gcgacggccg cgtggaaccc ggcaagatca
     2941 ccaagctgtt tctgttccac gggttgcgcc gttccgaggc gcaggaggcg cgggccggag
     3001 acatcgtggc cgtggccggg ctcgaatcac tgaccatcgg ggacacgttg accgcgccgg
     3061 atcgtccgga ggggttgccg ccgttgtcca tcggcgagcc cacgatctcc atggcgttca
     3121 tggtcaacaa ttcgccgttg gcgggccgcg aaggcaagtt cgtgacctcg cggcacttgc
     3181 gggaccggtt gctgcgcgaa ttgcggacca acgtggcctt gcgggtcgag gaatccggca
     3241 ccaccgacga gttcctggtg tcgggccgcg gggagttgca cctggcgatc ctcatcgaga
     3301 acatgcggcg cgagggctac gagttcgcgg tgtcgcgtcc gcaggtgatc ttcaagacca
     3361 tcgacggcga gacctgcgag cccatggaaa ccctgctcct cgacgtgccg gaatcctcgg
     3421 tcggggtggt gatggaagcg ctgggcccgc gtcgcgccga gttggagaac atggtcaacg
     3481 gcggcgacgg gtgggtgcac ctggcgttca cgatcccggc gcgcggcctg ttcgggtacc
     3541 gcagcgaata tctcaccgcc accaagggcg agggattgat caatcacgcg ttcgcgggct
     3601 accggccctc caagggcgac gtgcccacgc gcaatcgcgg ggtgctggtg gcgttggagc
     3661 cgggggaatc ggtcacgtac gcgctgcaca atatccagga gcggggcgcg ctgtttctcg
     3721 ggcccggcga gccgatctac gagggcatga tcatcggcga gcactcgcgg cccggggatc
     3781 tgatcgtcaa cccgtgcaag aagaaacacc tgaccaacat ccgttcgtcc ggggccgagg
     3841 acgcgctggt gctcacgccc ccgcgccggc tctcgctcga acaggcgatc gagttcatcg
     3901 aggacgacga gctggtggag atcacgccgc agtcgatccg gctccggaag cgctatttga
     3961 acggcgagga ccgcaagcgg cgacgcaacg cggcgtcggc ttgaccgggt tgcgcggagt
     4021 atgctacaca gaccgatact tgcctgacgg accggtaacg cgaccatgct gagcggggat
     4081 cttgaagagg gcctgacatt cgacgacgtg gccctgatcc ctgcccactc gaccgtcctg
     4141 cccaaggatg ccgacaccgg gacgcgcctc accaaacgca ttcgactcca tatccccatc
     4201 gtcagcgcgg cgatggacac cgtgaccgaa gccccgctcg cgatcgccct ggcccgcatc
     4261 ggggggatcg gcatcatgca ccgggtgctg agtcccgaga agcaggccga agaggtcgcg
     4321 aaggtcaaaa agtccgaaag cgggatcatc accgacccga tcacgatttc ccccgacgcc
     4381 acgatcaacg agacggtgct catcatggag cgccacgaaa tctccggcat tccggtcacg
     4441 gagcaacgca agctggtggg cattgtcacg cgccgcgatc tgcagttcga gaaacggatg
     4501 gacctcaggg tcgccgacgt catgaccaag ggcgaccggt tgatcaccac gcccgagggg
     4561 accacgctcg aagccgcgag cgcgatcatg cagcgccacc gcatcgaaaa actccccatc
     4621 gtcaacgacc gcttcgaatt gcagggcctc atcacggtca aagacctcaa gatggccatc
     4681 gagtacccca acgcggccaa ggacgaacgc gggcggctgc gcgtgggcgc ggccgtgggc
     4741 gtgggccgcg aagcgcacca gcgcgtggac gccctggtgg cggccggcgt ggacgtggtg
     4801 gtggtggaca ccgcgcacgg ccactcgcag gacgtgctgg aaatggtgca ggcggtcaaa
     4861 tcgaaatatc cgaacgtgga cgtgatcgcg ggcaacatcg ccaccgcgca ggccaccgag
     4921 gacctgatca aggccggcgc ggacgcggtg aaggtgggca tcgggccggg ctccatttgc
     4981 accac
//
LOCUS       DBNGTT010000438         6039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_998495, whole genome shotgun sequence.
ACCESSION   DBNGTT010000438 DBNGTT010000000
VERSION     DBNGTT010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_998495"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC16BC_09880"
     CDS             <1..311
                     /locus_tag="AC16BC_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3241970.1"
                     /translation="MGNAGQANYAASKAAIIGFTKSIAREYANRGVTANAVAPGFIKT
                     AMTDQLPEDVQTKLREQIPLGRLGAPEDIAQAVAFLASDAAAYVTGQVLHVNGGMWMG
                     "
     gene            393..623
                     /gene="acpP"
                     /locus_tag="AC16BC_09885"
     CDS             393..623
                     /gene="acpP"
                     /locus_tag="AC16BC_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3241971.1"
                     /translation="MAADDRVKKIITEQLGVDEEDVTPEANFVEDLGADSLDTVELVM
                     AFEEEFGIEIPDEDAEKILTVQQAVDYINEKL"
     gene            780..2015
                     /gene="fabF"
                     /locus_tag="AC16BC_09890"
     CDS             780..2015
                     /gene="fabF"
                     /locus_tag="AC16BC_09890"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3241972.1"
                     /translation="MTRRVVITGLGAVTPLGLDVEETWTALCAGRSGIGPITRFDTTD
                     FPVTIAGEVKGFDPVRHIDKKEVKKMDTFIHYALAAGQMAFADSGVRVDAAGTERVGV
                     YIGSGIGGLQAIEEWHTVLQEKGPKRITPFFIPMSIINMASGQLAIALGARGPNSAAV
                     TACATGNHCIGDAFRLIQRGDADVMIAGGTEAAITPLGVAGFAASRALSARNNDPQHA
                     SRPFDRDRDGFVIGEGAGVMVLESLESARKRNAKIYAEIVGYGSSADAYHITAPPEDG
                     EGAVRCMRSALADAGAKPSDVGYINAHATSTMADAIETRAIKTVFGADTRIPVSSTKS
                     MTGHLLGAAGGIEAVFSVLAIHREVLPPTINLENPDPACDLDYIPNTARRARVEVAMS
                     NSFGFGGVNACLVFRRFSA"
     gene            2045..2752
                     /gene="rnc"
                     /locus_tag="AC16BC_09895"
     CDS             2045..2752
                     /gene="rnc"
                     /locus_tag="AC16BC_09895"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3241973.1"
                     /translation="MSDAAATERLIERLAYRFRSPSLLDDALTHKSYLNEAPDPASRD
                     NERLEFLGDAVLDLVISEALLSRFPQAAEGTLSKLRARIVSEAALSQVAKRLELGGAL
                     RLGRGEELTGGREKPSLLADALEAVIAAVYADGGIDAARTVVLAAFSDLLDNLSRPEV
                     TDHKTELQEVCQRAFGGLPVYRVLGESGPDHHKQFEVELTIRGEVYGIGTGRSKKEAE
                     QRAAQHALQKLKHAPGA"
     gene            complement(2774..3496)
                     /locus_tag="AC16BC_09900"
     CDS             complement(2774..3496)
                     /locus_tag="AC16BC_09900"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HIR3241974.1"
                     /translation="MARRVGNIGVLASGGVESAALIAWALDRFSRVTPLYVRAGLRWE
                     PVERRWLGRLVRSIGSERLDAPVALDVPVRDLYGRHWSVTGRGVPDAASPDPAVYLPG
                     RNLLLLSKASVYAALHGLDALAIGPLGDNPFPDATPAFFTALESAIQRGLAAPIAIHT
                     PFRSLHKTELIARYRHVPWGVTFSCIRPVGLRHCGRCNKCEERRKAFTEAGVRDPTVY
                     AARAPGVARGPKSRRRSARPSR"
     gene            complement(3529..3951)
                     /locus_tag="AC16BC_09905"
     CDS             complement(3529..3951)
                     /locus_tag="AC16BC_09905"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR3241975.1"
                     /translation="MYKVTKRLTFCYGHRLMDYDGKCRVPHGHNGAVEIELEADQLDH
                     RGMVYDFSEIKSAVQTWLDRELDHRMLLRKDDPLVAALRGLGEPLVVMDENPTAEAIA
                     RLIYEFAESRGFPLTQVRLWETETSYATYERPRGSGAG"
     gene            complement(4112..4849)
                     /locus_tag="AC16BC_09910"
     CDS             complement(4112..4849)
                     /locus_tag="AC16BC_09910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3241976.1"
                     /translation="MRLEKRTVFVTGGAKRIGSQLARALAQRGANLVINYRYSSADAQ
                     AVVQELRRVGVDALAVPGDVSNANDVQRMVAGAMERFGRIDVLINNAAVYFKTPFAQL
                     TETEWDLTVDINLKGSFLCAKAVAGHMLAQGEGKIINFADWAGMRPYPEYLPYCVSKA
                     GVIALTQALAQELAPHVQVNCIAPGPILLPEDMDEDERQRVIRATPLKRIGSPEDIVQ
                     AVLFLIEGTDFATGGTYLIDGGRFVAS"
     gene            4948..5979
                     /gene="purM"
                     /locus_tag="AC16BC_09915"
     CDS             4948..5979
                     /gene="purM"
                     /locus_tag="AC16BC_09915"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3241977.1"
                     /translation="MAKETYRSAGVDIDAGDRTVELIKPLVKATFRPEVLADLGGFAG
                     LFRFDSAKYREPVLVSGTDGVGTKLRIAQLMRRHDTIGIDLVAMCVNDLIVQGAEPLF
                     FLDYFATGKLDNAVAEQVIRGIGEGCRQAGCALVGGETAEMPGMYGKGDYDLAGFTVG
                     VVEKSKIIDGSQVKSNDTLIGIASSGPHSNGYSLIRKIIEVRKVRLDQLLGSRPLGEV
                     LLAPTRIYVKPLLALMRELPVHAAAHITGGGLPGNVPRVLPADVRAVIDARAWKRPVI
                     FDWLQEMGGIEDFEMYRTFNCGIGMVLALDAADAEKALKLLAAAGETAAIIGHIETHP
                     GEDQVVILK"
BASE COUNT         1131 a         2050 c         1899 g          959 t
ORIGIN      
        1 tgatgggcaa cgcgggccaa gccaactacg ccgcgtccaa ggccgccatt atcggcttca
       61 ccaagagcat cgcgcgcgag tacgccaacc gcggcgtcac ggccaacgcc gtggcgccgg
      121 ggttcatcaa aaccgccatg accgatcagc ttccggaaga cgttcaaacg aaactgcgcg
      181 agcaaatccc gttgggtcgg ctcggcgcgc cagaggatat cgcccaggcc gtcgcgttcc
      241 tggcgtcgga cgccgcggcg tacgtcacgg ggcaggtgtt gcacgtcaac ggcggcatgt
      301 ggatgggata ggcgaccgag cctgagcggg cctgaacccg tcggctcatc atcgtcgccg
      361 aacaacagaa actaacagga ggagagtatc gtatggctgc ggacgatcgc gtcaagaaaa
      421 tcatcacgga acaactcgga gtggacgaag aagacgtcac gcccgaggcg aacttcgtcg
      481 aggacctcgg cgccgactcg ctcgacaccg tcgagttggt catggcgttt gaggaagaat
      541 tcgggatcga gatcccggac gaagacgcgg aaaaaattct gaccgtgcag caggcggtcg
      601 actacatcaa cgaaaagctc tgatttggcc gtgttctctc attatcatca gaagcctgtt
      661 cagcaacgcg ccagatgccc ccttcgagga cttccaccat tgcgaggtcg gtacgtgagc
      721 accggatgcg cagcggcaac gccgcaggtg gccgttgatg gacaggcgca gccattctca
      781 tgacccgccg cgtcgtcatc accgggttag gggctgtcac gcccttgggc ctcgacgtcg
      841 aagagacgtg gacggcgctg tgcgctggcc gatccggcat cggtcccatc acccgcttcg
      901 ataccaccga cttccccgtc accatcgcgg gagaggtcaa agggttcgac cccgtccggc
      961 acatcgataa aaaagaggtc aagaagatgg acacgttcat ccactatgcc ctcgcggccg
     1021 gccagatggc gttcgcggac tcgggggtgc gggtggacgc ggcgggcacc gaacgggtcg
     1081 gagtgtacat cggctccggg atcggcgggt tgcaggccat cgaagagtgg cataccgtgc
     1141 tccaggaaaa aggacccaag cggatcacgc cgttcttcat cccgatgtcc atcatcaaca
     1201 tggcctccgg ccaattggcg atcgcgctcg gcgcgcgcgg ccccaactcc gcggcggtca
     1261 cggcctgcgc cactggcaat cactgcatcg gggacgcgtt ccgtctgatc cagcgcggcg
     1321 acgccgacgt gatgatcgcg ggcggcaccg aagccgcgat cacgccgttg ggcgtggcgg
     1381 gattcgccgc gtcgcgggcg ctctcggcgc gcaacaacga cccgcagcac gcgtcgcgtc
     1441 cctttgaccg cgaccgcgac gggttcgtga tcggcgaagg cgccggggtc atggtgctcg
     1501 agtcgctgga gtcggcccgc aaacggaacg ccaagattta cgcggagatc gtgggctacg
     1561 gatcgtccgc ggacgcctac cacatcaccg cgccgccgga ggacggcgaa ggcgcggtcc
     1621 gctgcatgcg gtccgcgttg gccgacgcgg gagccaagcc ctccgatgtc gggtacatca
     1681 acgcgcacgc cacctcgacc atggccgatg cgatcgaaac gcgggcgatc aaaaccgtgt
     1741 tcggcgcgga caccaggatc ccggtgagtt ccaccaagtc catgaccggc cacctcctgg
     1801 gcgcggccgg cggcatcgaa gcggtcttct cggtgctggc gattcaccgc gaagtgttgc
     1861 ccccgaccat caatctggaa aacccggacc cggcgtgcga tctggattac atccccaaca
     1921 ccgcgcgccg cgcgcgcgtg gaggtcgcca tgtccaactc gttcgggttc gggggcgtca
     1981 acgcgtgttt agtgtttcgt cgatttagcg cctagcgcgc ccactcccgt cccggtcgca
     2041 ccgcatgtcc gacgccgcgg ccactgaacg cctgatcgaa cggctcgcct accggttccg
     2101 gtccccgagc ctgctcgatg acgcgctcac tcacaagtcg tatctgaacg aagcgcccga
     2161 tcccgcgagc cgggacaacg agcgcctgga gtttctgggg gacgcggttc tggacctggt
     2221 gatcagcgaa gcgttgctgt cgcggtttcc gcaggccgcg gaaggcacct tgtcgaagct
     2281 gcgcgcgcga atcgtcagcg aggccgcgct gagccaggtg gccaagcggc tggagctggg
     2341 cggcgcgctc cgcctgggcc gcggcgagga gctgaccggc ggccgcgaga aaccgtccct
     2401 gctggccgac gcgctcgaag ccgtgatcgc tgccgtctac gcggacgggg ggatcgacgc
     2461 ggcccgtaca gtggtcctgg cggcgttctc ggacctgctc gacaatctct cccgccccga
     2521 ggtgaccgac cacaagaccg aacttcagga ggtatgccag cgggcgttcg gcggactacc
     2581 ggtgtaccgc gtgttgggcg agtcggggcc ggatcaccac aaacaattcg aggtcgaact
     2641 caccatccgc ggcgaggtct acggcatcgg gaccggacgc agcaaaaaag aagcggaaca
     2701 gcgagccgcg caacacgcgc tgcaaaaact caagcacgcg cccggcgcat agcccaccga
     2761 tcaccatcca cgctcaccgc gaaggacgag cggatcgccg ccggctcttc gggccgcgcg
     2821 caacacccgg ggctctcgct gcatagaccg tcgggtccct aaccccggcc tccgtaaagg
     2881 ctttgcgtcg ctcctcgcat ttgttgcacc gcccgcagtg ccggagcccg accggacgga
     2941 tacaggaaaa cgtcaccccc cacgggacat gacggtatcg cgcgatcagt tccgtcttgt
     3001 gcagggatcg aaacggggtg tggatcgcga taggcgcggc caagccgcgt tggatcgccg
     3061 actccaaggc cgtgaaaaac gccggggtcg cgtccggaaa cgggttgtcg ccgagcggcc
     3121 cgatcgcgag cgcgtcaaga ccgtggagcg cggcatacac cgacgccttg ctcaggagga
     3181 gcaggttgcg tcccggcagg tagacggccg ggtccgggct cgccgcatcc ggcaccccgc
     3241 ggccggtcac gctccagtgc cggccgtaca gatcccgcac cggcacgtcc agcgcgaccg
     3301 gggcgtcgag tcgctcggag ccgatgctcc tcaccagccg ccccagccag cgacgctcga
     3361 ccggctccca acgaaggccg gcccgcacgt acaacggggt gacgcggctg aaccgatcca
     3421 gggcccacgc gatcagcgcg gcgctctcca ccccgccgct cgcgagcacg ccgatgttcc
     3481 cgacccgccg cgccaccgga ggttaccgga gcgcgcgacg ccgcgcggtc atccggctcc
     3541 cgacccgcgc ggacgctcgt acgtcgcgta cgacgtttcg gtctcccaca accggacctg
     3601 cgtcaacggg aatccgcggg actccgcgaa ttcgtagatc aggcgcgcga tcgcctcggc
     3661 ggtcggattc tcgtccatca ccaccaaggg ctcgcccagg ccgcgcaagg ccgcgaccag
     3721 cgggtcgtcc tttcgcagca acatccgatg gtcgagctcg cggtccaacc acgtttgcac
     3781 cgcggacttg atctcggaaa aatcatagac catgccccgg tggtcgagtt gatccgcttc
     3841 caactcgatc tccaccgcgc cgttgtgacc gtgcggcacc cggcacttcc cgtcatagtc
     3901 catcaatcga tggccgtaac agaatgtcag acgtttagtg actttataca tgaacaccgt
     3961 gtcctcgccc gcccctagca cgttgccgaa agaccttgtg gaagtccttc gacgagctca
     4021 ggacgaacgg atcgacaatg aactgctctg cgaaaactcc gttcgtggtg agcgtgtcga
     4081 accacgctcc atacgttgtc agcatacccg cctacgaggc aacgaaccgc cccccatcaa
     4141 taaggtatgt gccgccggtc gcaaaatccg tcccttcgat caaaaacaat acagcctgga
     4201 caatgtcctc cggcgaccca atacgtttca gcggggtcgc gcggatcacg cgctggcgtt
     4261 cgtcctcgtc catgtcttcc ggcaggagga tcggtcccgg cgcgatgcaa ttgacttgga
     4321 cgtggggcgc cagctcctgg gcgagcgcct gcgtcagcgc gatcaccccg gccttggaca
     4381 cgcaatacgg caggtactcc gggtacggcc gcatccccgc ccagtccgca aaattgatga
     4441 tcttgccctc gccctgcgcc agcatgtggc cggcaaccgc cttggcgcac aggaaactgc
     4501 ccttgagatt gatgtccacc gtgaggtccc attccgtctc ggtgagctgc gcgaacgggg
     4561 tcttgaagta caccgcggcg ttgttgatta agacgtcgat gcgcccgaac cgctccatcg
     4621 cgcccgccac catccgctgc acgtcgttcg cgttggacac gtcgcccggc accgccagcg
     4681 catccacccc cacgcgccgc agctcctgca ccaccgcctg cgcgtccgcg ctggaatacc
     4741 ggtagttgat caccagattg gccccgcgtt gcgccaatgc gcgagccaac tgactgccga
     4801 tccgcttggc cccgccggtg acgaacacgg ttcgcttctc aagccgcata gggagccgat
     4861 cttaaaggca agccggaacg ggtgtcaacg ccaaattgat ctttcccggg tgttcttgta
     4921 tcatgacgcg ggatcggacc tcctgttgtg gccaaagaaa cctaccgcag tgccggcgtg
     4981 gacatcgacg ccggagaccg caccgtcgag ctgatcaaac ccctggtgaa ggcgaccttc
     5041 cgccccgagg tgctcgcgga cctgggcggt ttcgccggct tgttccggtt cgacagcgcg
     5101 aagtaccgcg aacccgtgct cgtctcgggc accgacggcg tgggcaccaa actccgcatc
     5161 gcgcaattga tgcgccgcca cgacaccatc ggcatcgacc tggtggcgat gtgcgtcaac
     5221 gacctcatcg tgcagggcgc cgagccgctg ttcttcctcg actacttcgc caccggcaag
     5281 ctcgacaacg ccgtggccga gcaggtgatc cgcggcatcg gcgagggctg ccgccaggcc
     5341 ggctgtgcgc tggtcggcgg cgaaaccgcg gagatgccgg gcatgtacgg caagggcgat
     5401 tacgacctcg ccggtttcac cgtgggcgtg gtcgaaaaat ccaaaatcat cgacggttcg
     5461 caggtcaaga gcaacgacac cctcatcggc atcgcctcca gcggaccgca ttccaacggc
     5521 tattcgctga tccgcaagat catcgaggtg cgcaaggtca ggctcgacca gctcctcggc
     5581 agccgtccgc tgggagaagt gctgctggcg ccgacgcgca tttacgtcaa accgctgctg
     5641 gcgctgatgc gcgagctgcc ggtgcacgcg gcggcgcaca tcaccggcgg cggcctgccc
     5701 ggcaacgtcc cgcgcgtgct gccggcggac gtgcgcgccg tcatcgacgc gcgcgcctgg
     5761 aagcgccccg tcatcttcga ctggctgcag gaaatgggcg gcatcgagga cttcgagatg
     5821 tatcgcacct tcaactgcgg catcggcatg gtgctggcac tggacgcggc ggacgccgag
     5881 aaggcgctca agctgctggc cgccgccggc gaaaccgccg cgatcatcgg ccacatcgag
     5941 acccacccgg gagaagacca ggtcgtcatc cttaaataat taatataatg acaagacgaa
     6001 cggagcgaag cggagtaggg ctactactct tatgtcaaa
//
LOCUS       DBNGTT010000439         6953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_998748, whole genome shotgun sequence.
ACCESSION   DBNGTT010000439 DBNGTT010000000
VERSION     DBNGTT010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_998748"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..700
                     /locus_tag="AC16BC_09920"
     CDS             89..700
                     /locus_tag="AC16BC_09920"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3241978.1"
                     /translation="MLGHERDLASAGYATLAGIDEAGRGPLAGPVVAAAVVLPWSRPI
                     PGVRDSKVVPPRERERLYYEIIHQAVAIGIGVVSVGDIERINILRATVKAMEQAVFHL
                     SCQPDALLIDAVSLSGVALPQRAIVRGDATSYLIAAASIVAKVTRDRFFVDYNVRYPD
                     YDFAAHKGYGTPGHLARLTTLGPCPIHRRTFRPVHGGAARHAE"
     gene            690..1079
                     /locus_tag="AC16BC_09925"
     CDS             690..1079
                     /locus_tag="AC16BC_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR3241979.1"
                     /translation="MRSEAGALGARGEHMASDVLVRRGYRIIERNCRSRWGELDLVAY
                     DGDTLVFVEVKARRSSQYGEPAYAVDRRKQTRLIRLAERYLLRRRLGEPPCRFDVVLV
                     EERTMLPPRVEVIANAFDASAGERRPC"
     gene            1073..1456
                     /locus_tag="AC16BC_09930"
     CDS             1073..1456
                     /locus_tag="AC16BC_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241980.1"
                     /translation="MLSGINTDIEHGGKTYHVQTEDGGEHNPVIVTHLFAGGAILSSQ
                     RVSYADVIKAGPNPDAVRELMKQQHQTMIKSLSSKDGAGAAAPLAAPPRAEVAPPPTA
                     TLPKGRRSLDDVIDEYLQSHRGHRS"
     gene            1456..2070
                     /locus_tag="AC16BC_09935"
     CDS             1456..2070
                     /locus_tag="AC16BC_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3241981.1"
                     /translation="MPTPALFGLRGEAPQARTAGAFPLGVQIMDVWKSYQPGLPVLRE
                     VSLQIGKGDLVVVEGPTGAGKSTLLHLVAGIERPDAGRIAVGTPVRDRRVRVYETLKA
                     FGLESRRNDYPDQLSAGEAQCVGLARALAARPAVVLADEPTALLDRRSASSVLLVLRD
                     IHARGATVLITSHDPDMASRLGGRRVLLRDGRVDEPPAARRGTA"
     gene            2067..2930
                     /locus_tag="AC16BC_09940"
     CDS             2067..2930
                     /locus_tag="AC16BC_09940"
                     /inference="COORDINATES: protein motif:HMM:NF037183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR3241982.1"
                     /translation="MKAAARGPTTWVTLTTAVVLTLAGGCWLVAWNAWLLMRGWRGDV
                     KVVVYLTDTASEQEVEALRTAIANEPAVESLRLISQSEAEEAFLRFMQLDRSALEGVG
                     EDAFPASIEVSMKEEGQVPDMMARLAARWTAFAGVEDVRYGESLIRDLSRAARVVSVL
                     GAIIGTALLSGAAVVIGVMIHISLRHRAAEVSLLRLFGASESVIVKPFLWEGVTIGLV
                     GGLLASGVLLGGWLAIRQRWGMAFEGLLAAWVDRLEFPLGLLPGLIALAVVVGGLGSF
                     VAVRRVHHSMP"
     gene            2893..4110
                     /locus_tag="AC16BC_09945"
     CDS             2893..4110
                     /locus_tag="AC16BC_09945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR3241983.1"
                     /translation="MWRSGGFTTRCRKHPGIGGAAVLGAAVFAWCVIGLGTGWAKPSL
                     ADRIADERRELAALQQELDRIKKQRDLVRSKERSVAERLAESERVLAITRRALRLTEV
                     NVDRKDRDLAESVSTLAERRQRAEETRRLARTRVRELAKWKGVYDGTFVVAAAPSEMA
                     MRYDMVRRVVDRDRRLVRDAQAAAVAVSEQVAELERLRRDLEAVRAEERVALGEVLTE
                     RESRRRLLSELRTERIAYTRSIQDLEEASRRLEALIDDLARAARGPHQGVGLAKLKGR
                     LIWPIQGEVVGLFGRQKHPRFQTYVDRKGIDIKIAQREAIRAVADGKVAYASPLKGYG
                     NVVIIDHGDQYLSLYANASSIRVHPGDDVGAGEPLGEASAEGGDDRMYFELRHGKNPL
                     DPLRWLLTRGGME"
     gene            4107..5468
                     /locus_tag="AC16BC_09950"
     CDS             4107..5468
                     /locus_tag="AC16BC_09950"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3241984.1"
                     /translation="MTDHRRRGWIPFGVVLVFVAGLLSGRLLEGKGLAQQAETYEELR
                     PFTEVLNQVQRNYVEETKTRDVVYGAIRGLLNTLDPHSSFMPPDVYKEMQVDTKGEFG
                     GVGIQIGLKDDRLTVIAPIEDTPASRAGIRAGDKIVKIDGAPTKDVTLMDAVNKMRGV
                     KGTKVTLTLEREGTAKPIEVALTRETIKIQSTRHKMLDGGIGYVRLTQFQEQSGPDLA
                     KALNSLRDQKAEAVILDLRNNPGGLLTAAVEVSEQFLPKGKVVVSIKGRQGKPEEYAA
                     DGEHPILDMPLIVLVNEGSASASEIVAGAMQDWGRAVVVGTTTFGKGSVQTVIPLSDG
                     SALRLTTAKYYTPKGRSIQNTGIAPTIVVRNAVKGEVKRQVVRERDLERHLQNETASP
                     TPEESQEPAELSPIPDVPVDGAAEEDLQLQKAIELLKSWAVFKGLLDQPQPPTEASQG
                     SST"
     gene            5582..5875
                     /locus_tag="AC16BC_09955"
     CDS             5582..5875
                     /locus_tag="AC16BC_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3241985.1"
                     /translation="MGAETATRKKVNFKPLKERVFVSYSEEQERTSGGLYIPDAAKEK
                     PQRGKVEAVGGEVKSVKVGDEILFDKYSGSKISFDGADYLIVKEDEILGVIEK"
     gene            5924..>6953
                     /gene="groEL"
                     /locus_tag="AC16BC_09960"
     CDS             5924..>6953
                     /gene="groEL"
                     /locus_tag="AC16BC_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3241986.1"
                     /translation="MAKQMVFSEDARASILKGVNQLSNAVKATLGPKGRNAVIEKKFG
                     APTITKDGVTVAKEVELKDPYENMGAQLVKEVASKTSDVAGDGTTTATVLAQAIFREG
                     VKSVSAGANSMDIKRGIDKAVEVVLDELKKISRPCQTKKEIAQIGTISANNDKAIGDL
                     IAEAMEKVGKDGVITVEEAKSMSTSLDVVEGMQFDRGYLSPYFVTDPERMEASLEDAL
                     LLINEKKVSTMKDLLPILEQVARMGKPLLIIAEEVEGEALATLVVNKLRGTLNVAAVK
                     APGFGDRRKAMLEDIAILTGGQVISEDLGLKLENVKITDLGRAKRVTIDKDNTTIVEG
                     AGDSSKIQG"
BASE COUNT         1300 a         1967 c         2510 g         1176 t
ORIGIN      
        1 ggcgaggtct cggtcgtcgg cgagacggtc gagaccgaaa cgctccccgt gtcctgacga
       61 attcggatcc tgccgccttc ggtcgcgggt gctcgggcac gaacgggatc tggcgtctgc
      121 ggggtacgca accctcgccg ggattgacga ggcggggcgc ggtccgctgg cggggccggt
      181 ggtggccgcc gcggtggtcc ttccctggag ccgtccgatc cccggcgtgc gggattccaa
      241 agtggtgccc ccgcgcgagc gggaacggtt atactacgag atcatccatc aagccgtggc
      301 catcgggatc ggcgtggtga gtgtggggga tatcgagcgg atcaatatcc tccgggctac
      361 ggtcaaggcc atggaacagg ccgtcttcca tctcagttgc cagcctgatg cgctgttgat
      421 cgacgccgtg tcgctcagcg gcgtcgcact gccccagcgc gcaatcgtcc ggggcgacgc
      481 taccagctac ctcatcgccg ccgcgtcaat cgtcgccaaa gtgacgcgcg atcgattttt
      541 tgtagactac aacgtgcggt acccggacta tgatttcgcc gcgcacaagg ggtacggaac
      601 gcccggtcat ttggcgcgct tgacgaccct cggcccgtgt cccatccatc ggcgcacgtt
      661 tcggccggtt cacggaggcg ccgcgcgcca tgcggagtga ggccggcgcg ctgggcgcgc
      721 gcggcgaaca catggcatcc gacgtgttgg tccgacgcgg gtatcggatc atcgagcgca
      781 actgtcggtc gcggtggggt gaactggacc tggtggcgta cgacggggac acgctggtgt
      841 tcgtcgaggt caaagcgcgg cgcagctcgc agtatgggga gccggcctat gccgtggatc
      901 gccgcaagca gacacggctg attcggctgg ccgaacggta tctgttgcgt cgtcgcctgg
      961 gagagccgcc gtgtcgattc gatgtggtgt tggtcgagga gcgcacgatg ttgccgcctc
     1021 gggtggaagt gatcgccaac gcgttcgacg cgtcggcggg tgagcggagg ccatgttaag
     1081 cggaatcaat acggatattg agcacggcgg gaaaacctat cacgtccaga ccgaggacgg
     1141 cggggagcac aacccggtca tcgtcaccca tctgttcgcg ggcggcgcga tcttgtcgtc
     1201 gcagcgggtc tcgtacgccg atgtgatcaa agcgggcccc aaccccgatg cggtgcgcga
     1261 actcatgaaa cagcagcacc agacgatgat caaaagtctg tcgtccaagg acggcgccgg
     1321 cgcggcggcg ccgctggcgg ccccgccccg cgcggaggtc gcgcctccgc ccacggcaac
     1381 cttgcccaag gggcgtcgct cgctcgatga cgtcatcgac gagtacctgc agtcccatcg
     1441 tggacatcgg tcctgatgcc caccccggcg ttattcgggc tccgggggga ggcgcctcag
     1501 gctcgaaccg cgggcgcctt tcccctgggc gtccagatca tggacgtctg gaagtcctat
     1561 caacccggtc tcccggtgct tcgggaggtt tcgttgcaga tcgggaaggg tgatctggtg
     1621 gtggtggaag gcccgaccgg ggcgggtaaa agcacgttgc tgcatctcgt ggccgggatt
     1681 gaacgcccgg atgccgggcg gatcgccgtg ggcaccccgg tccgagaccg ccgagtccgt
     1741 gtgtacgaaa cgttaaaagc ctttgggttg gagagccgtc gcaacgatta ccccgatcaa
     1801 ctctccgcgg gcgaggcgca gtgtgtcggc ctggcgcggg cgctggcggc ccgcccggcc
     1861 gtggtgttgg cggacgagcc gaccgcgctc ctggatcgtc gcagcgcatc ctcggtgctc
     1921 ttggttttgc gggatatcca tgcgcgcggc gccacggtcc tcataacgtc gcatgatccc
     1981 gacatggcga gccggctcgg cggccgacga gtgctgttgc gcgacggccg ggtggatgag
     2041 ccgccggcgg cgcgcagggg gacggcgtga aggcggcggc ccgtggtccc accacgtggg
     2101 tgaccttgac caccgccgtg gtgttgacgc tggccggcgg ctgttggttg gtggcgtgga
     2161 acgcgtggtt gctgatgcgt gggtggcgcg gcgacgtcaa ggtggttgtg tatcttacgg
     2221 ataccgcttc ggaacaggaa gtcgaggcct tgcgcaccgc gatcgcgaac gaacccgcgg
     2281 tcgagtccct gcggctgatc tcgcagtccg aggccgaaga ggctttcttg cggtttatgc
     2341 agctggatcg ttccgcgctg gaaggcgtgg gcgaagacgc gtttcccgcg tccatcgaag
     2401 tgtccatgaa ggaggaaggt caggtcccgg acatgatggc gcgcctggcc gcgcgctgga
     2461 ccgcgttcgc cggggtggaa gacgtgcggt acggggagag cctcattcgt gacttgagtc
     2521 gcgccgcccg cgtggtatcc gtgctcggcg ccatcatcgg cacggccctg ttgagcggcg
     2581 ccgcggtggt catcggggtg atgatccata tttcgctccg gcatcgcgcc gcggaagtgt
     2641 cgctgctgcg cctgttcggc gcgtccgaga gcgtcattgt caaaccgttt ctgtgggagg
     2701 gcgtgaccat cggtctggtt gggggcctgc tcgcctccgg ggtgttgctc ggcgggtggt
     2761 tggcgatccg ccagcgatgg ggcatggcgt tcgaggggtt gttggcggcg tgggtcgatc
     2821 ggttggagtt cccgctgggg ctccttccgg ggctgatcgc gctcgcggtc gtggtcggag
     2881 gactcgggag ctttgtggcg gtccggcggg ttcaccactc gatgccgtaa acatccgggc
     2941 atcggcggcg cggcggtcct cggcgcggcc gtgttcgcat ggtgcgtgat cggcctcggc
     3001 actggctggg ccaagccgtc gttggcggat cggatcgccg acgaacgtcg agagttggcc
     3061 gcgctgcaac aggaattgga ccggatcaag aagcaacggg acctggtcag gtcgaaggaa
     3121 cggtcggtgg ccgaacggtt ggcggaatcg gaacgcgtgc tcgcgatcac gcggcgcgcg
     3181 ctgcggttga cggaggtgaa cgtggaccgg aaagaccgag accttgccga gtccgtgagc
     3241 acgctcgcgg agcgccgaca gcgcgccgag gagacgcggc ggttggcgag gacccgggtt
     3301 cgcgagttgg cgaagtggaa gggcgtctac gacgggacgt tcgtggtggc ggccgcacca
     3361 tcggagatgg ccatgcggta tgatatggtg cgtcgtgtgg tcgatcggga ccgtcgtctg
     3421 gtgagggacg cgcaggcggc ggcggtcgcg gtgtcggaac aggtcgcgga gctggagcgg
     3481 ttgcgacggg acctggaggc ggttcgcgcg gaggagcgcg tcgcgctggg cgaagtgctg
     3541 accgaacggg agtcgaggcg gcgattattg agcgagctcc ggaccgaacg catcgcctac
     3601 acgcgttcga tccaggattt ggaggaagcg tcgcggcgct tggaagcgct catcgatgac
     3661 ctggcgcggg cggcgcgggg accgcatcaa ggggtggggc tcgcgaagct caagggtcgg
     3721 ttgatctggc ccatacaggg cgaggtggtc gggctgttcg gtcgtcaaaa acacccgcgc
     3781 tttcagacgt acgtggaccg aaaaggcatc gacatcaaga tcgcgcaacg cgaggccatc
     3841 cgggcggtgg ccgacggcaa ggtggcgtat gcgagtcctc tcaaggggta tggaaacgtc
     3901 gtcatcattg accatgggga tcagtatttg tcactctacg caaacgccag ttcgatccgc
     3961 gtgcatccgg gtgacgacgt gggggcgggg gagccgctcg gcgaagcgtc cgccgagggc
     4021 ggcgatgacc gcatgtattt cgagctgcgg cacgggaaaa atccgctcga tccgctgagg
     4081 tggctgttga cacgaggagg aatggaatga ctgatcatcg tcgacggggc tggatcccat
     4141 tcggcgtcgt actggttttc gtcgccgggc tgctctccgg gcggttgttg gagggtaaag
     4201 ggctggccca gcaggccgaa acctacgaag agctgcggcc gtttaccgaa gtgttgaatc
     4261 aggtccagcg caactacgtg gaggaaacca agacccgaga cgtggtctac ggcgcgatcc
     4321 gcgggttgct caacaccctg gaccctcact cgtcgtttat gccgccggac gtgtacaagg
     4381 agatgcaggt cgacaccaaa ggggagttcg gcggcgtggg cattcagatc gggttaaagg
     4441 acgatcgtct gaccgtgatc gccccgatcg aggacacgcc cgcgtcccgg gcggggatcc
     4501 gggccggaga caaaattgtg aagatcgacg gcgcgccgac caaggacgtg acgctgatgg
     4561 acgcggtcaa caagatgcgg ggcgtcaagg ggaccaaggt gacgctcacg ctcgaacgcg
     4621 aggggaccgc caagccgatc gaagtcgcgt tgacgcggga aacgatcaag attcaaagca
     4681 cccggcacaa gatgcttgac gggggcatcg gttacgtccg gctcacgcag tttcaggagc
     4741 agagcgggcc ggatttggcc aaagccctga actcgctgcg cgatcaaaag gccgaggcgg
     4801 tgatcctcga tctgcggaac aatccgggcg ggctgttgac cgcggcggtc gaggtctccg
     4861 agcagttcct gcccaagggg aaggtcgtgg tctcgatcaa gggacgccaa ggcaagcccg
     4921 aggagtacgc cgcggacggc gagcatccga tcctcgacat gccgctgatc gtgctggtca
     4981 acgaaggcag cgccagcgcg tcggagatcg tggcgggcgc catgcaggac tgggggcggg
     5041 cggtggtggt gggcacgacc acgttcggca agggatcggt gcagacggtg atcccgctca
     5101 gcgacgggtc cgcgcttcgt ctgaccacgg cgaagtacta tacgcccaaa ggccgctcga
     5161 tccagaatac cgggatcgct ccgaccattg tcgtgcgcaa tgcggtcaag ggagaagtga
     5221 agcgtcaagt ggtccgcgag cgtgatttgg agcggcacct ccagaacgag accgcctcgc
     5281 ctactccgga ggaatcgcag gaaccggcgg aattgagtcc gatccccgat gtgcccgttg
     5341 acggtgccgc cgaggaggat ctccagctcc agaaggccat cgagctgttg aagagctggg
     5401 cggtttttaa gggcttgttg gaccaacccc agcctcccac cgaggcgtcc cagggaagtt
     5461 cgacatagtc ggggggcgat aagttgagcc cccttgattt ttgcggagct gatcgctagt
     5521 attgtcagca ctcaaatctg gtgagtgctg atggtattca actgacacaa ggaggaggaa
     5581 cgtgggcgca gaaacagcaa ctcggaaaaa ggtcaacttt aagcccctga aagagcgggt
     5641 gtttgtcagc tactcggaag aacaggagcg gaccagcggc ggactctaca tcccggatgc
     5701 ggccaaagag aaaccgcagc gaggcaaggt tgaggccgtg ggcggtgagg tcaaatccgt
     5761 gaaggtcggc gacgaaatcc tgttcgacaa gtactcgggg agcaagatct cgtttgacgg
     5821 tgcggactac ctgattgtga aagaagacga gattctcggg gtcatcgaga agtaaccacc
     5881 acgactgacg atcgatcgag acatcgaaag ggaggagtag aagatggcga agcaaatggt
     5941 gttcagcgaa gacgcgcggg cgtcgattct caagggcgtg aatcagctca gcaacgccgt
     6001 gaaggcgacg ctcggcccca agggacgcaa cgcggtgatc gaaaagaagt tcggcgcgcc
     6061 gaccatcacc aaggacggcg tgaccgtggc gaaggaagtg gagttgaagg acccgtacga
     6121 aaacatgggc gcgcaactgg tcaaggaagt ggccagcaag accagcgacg tggcgggcga
     6181 cggcaccacc accgcaaccg tgttggcaca ggcgatcttc cgcgaaggcg tgaaaagcgt
     6241 gtcggccggc gcgaactcga tggacatcaa gcgcgggatc gacaaagcgg tggaagtcgt
     6301 gctcgacgag ctgaagaaaa tcagccggcc gtgccagacc aaaaaagaga tcgcgcagat
     6361 cggcacgatc tccgcgaaca atgacaaggc gatcggcgat ctcattgccg aggccatgga
     6421 gaaggtcggc aaagacggcg tcatcacggt cgaggaagcc aagagcatga gcacctccct
     6481 ggacgtggtg gaggggatgc agtttgaccg cggctacctc tcgccctatt tcgtcacgga
     6541 tccggagcgc atggaagcct cgcttgaaga cgcgttgctc ctgatcaacg agaagaaggt
     6601 cagcaccatg aaggacctgc tgccgattct cgagcaggtg gcgcggatgg gcaagccgtt
     6661 gttgatcatc gcggaagaag tcgagggcga ggccttggcc acgctcgttg tcaacaagct
     6721 ccgcggcacg ttgaacgtcg cggcggtgaa ggcccccgga ttcggcgacc gacgcaaggc
     6781 gatgctggaa gacatcgcga tcctcacggg tgggcaagtg atctccgagg atctcggctt
     6841 gaaactggaa aacgtgaaga tcacggacct cggccgcgct aagcgcgtca cgatcgacaa
     6901 ggacaacacc acgatcgtgg aaggcgcggg cgactcttcc aagatccagg gcc
//
LOCUS       DBNGTT010000440         3691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2404035, whole genome shotgun sequence.
ACCESSION   DBNGTT010000440 DBNGTT010000000
VERSION     DBNGTT010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2404035"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="AC16BC_09965"
     CDS             complement(<1..485)
                     /locus_tag="AC16BC_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3241987.1"
                     /translation="MPSERDIVIVIGGGFAGLAAGVALAERGYRVTVLEKRSRLGGRA
                     SSFVDPATGEVLDNGQHVFLRSYQATIRFLATIGTLDQLVFQPRLSLEVVGLNGVRTR
                     VTAALLPAPWHMAVGLMRASGLPWDARRKGLELGWHLWRRGADDTEGLTVAEWLDRHQ
                     QP"
     gene            complement(652..1053)
                     /locus_tag="AC16BC_09970"
     CDS             complement(652..1053)
                     /locus_tag="AC16BC_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3241988.1"
                     /translation="MYRCPCCQQPSPKTQIDITNVQGAVNADPLTDQDTFKRTYIGHG
                     TQSSVFVFQGHRGPFRKHIHVTHDEIGYVLDGQGTVTVGGLTREVRPGDIWIIPANTP
                     HAAQFDSDRVQVLFMSSPMDDPAHPDRVWLD"
     gene            complement(1106..1486)
                     /locus_tag="AC16BC_09975"
     CDS             complement(1106..1486)
                     /locus_tag="AC16BC_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241989.1"
                     /translation="MMRTLFGVGLLLAWMLGAVGPTMAGDTKISNIETLFAQKDKLSG
                     QRIQISGKVVKVNNGIMGKNFFHVQDGSGKSGANDLTVTTQDIVQVGDQVTVTGVVTV
                     NRDFGAGYSYPLIVEEATVTPAGH"
     gene            complement(1552..2190)
                     /locus_tag="AC16BC_09980"
     CDS             complement(1552..2190)
                     /locus_tag="AC16BC_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3241990.1"
                     /translation="MHHLITIWFSWVHDWGYLGIVVLMAMESTIFPVPSELVIPPAAY
                     WAAQGRYTLWGVVLAGTVGSVIGAGLTYWVSRAVGRPLIVRFGRYAGVTEAKLVRAER
                     WLARYEAGGVFFARLLPVVRHLIGIPAGIVRMPIGLYLLVTTAGSALWCTVLAWFGAH
                     VIGDQPQLLDDPGHMIAVLQSRGWWFAGLALLLCVLYVVVMRLTAKPASPSA"
     gene            complement(2197..3459)
                     /locus_tag="AC16BC_09985"
     CDS             complement(2197..3459)
                     /locus_tag="AC16BC_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR3241991.1"
                     /translation="MAQRAARPPSSRPSDRPNGIIERYLKLSRLSYPVPAHGKTLPYT
                     LGGIVFLGFLLLFASGFLLAQFVDPSPERAYQSVKALGERVPGGSWVRALHYWTAQAA
                     VVALILHVLRIVFSGAYKAPRLVTWYVGLALLGLGVFGSYFSGTVLKWDQESFDALKH
                     YEAGLTRLGPLGAFLGSTDAVSLNVKLYASHVSILPLVIVAFLAAHFYLVNTHSLSPL
                     PFGEDSARESVPKERMTGTMTEHVKGIVLFGGIYFGVTALLAAIVPAPLGDPVTGEEM
                     SLKPPWPFLWLYGLENLTGKMDTMFHALGALFLALAVLPLVDRGPERNPARRKGVLAV
                     AGIALISMIGLTAYAAIAPPQMHHHEHGSGHDAPAMSHDAGTHDTMPDTTVPHDASPH
                     DRGTPDAAVPDPSPADNHHDETPREHAP"
     gene            complement(3469..3542)
                     /locus_tag="AC16BC_09990"
     tRNA            complement(3469..3542)
                     /locus_tag="AC16BC_09990"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3508..3510),aa:Gln,seq:ctg)
BASE COUNT          705 a         1186 c         1197 g          603 t
ORIGIN      
        1 ggctgctggt ggcgatcgag ccactcggcc acggtcaggc cctcggtgtc gtccgcgccg
       61 cgccgccaca gatgccagcc cagttcgagc cccttgcgtc gcgcgtccca cggcaggccg
      121 cttgcccgca tcaatccgac cgccatgtgc cacggcgccg ggagcaacgc ggcggtcacc
      181 ctcgtgcgca ccccgttgag gcccacgact tccagcgaca aacggggttg aaacacgagt
      241 tgatcgagcg tgccgatggt ggcgagaaag cggatcgtcg cctgatacga gcgaaggaac
      301 acgtgttgcc cgttgtccag cacttcgccg gtagcgggat ccacgaagga tgacgcgcgg
      361 ccgcccagcc gcgaacgctt ctccagcacc gtgacccgat acccccgctc cgccagggcc
      421 accccggccg ccagccccgc gaacccgcct ccgatgacaa tgacgatgtc acgttctgaa
      481 ggcattttct cctgtgttca gcatattgag ataatggtaa atggtgggcc gtcgagagtg
      541 ggtcagatgc aaggcggagg gagaccttgc gccggagcgt actgggtcgt acgtgaggac
      601 gcgaggtcga aacgacaacg ccgcagatgg cccgctatcg acagcccacg gctagtcgag
      661 ccaaacccgg tccggatgcg ccggatcatc catcggcgac gacataaaca acacctgaac
      721 ccggtcgcta tcaaactgcg ccgcgtgcgg agtgttcgcg ggaatgatcc agatgtcgcc
      781 gggacgcacc tcgcgggtga ggccccccac cgtgacggtc ccttgtccgt ccaagacgta
      841 gccgatctcg tcgtgggtga cgtggatgtg cttgcgaaac ggtccccggt gcccctggaa
      901 cacgaacacg ctggattggg tgccgtgccc gatataggtg cgcttgaacg tgtcctggtc
      961 ggtgagcggg tccgcgttga ccgcaccttg cacgttcgtg atatcaatct gggtcttcgg
     1021 gctcggctgc tgacagcacg gacaccgata catacgtggc tcctcccgtg tgcttgctcc
     1081 cgacggtcgc aggtcccggc cgcgatcaat ggccggccgg cgtcaccgtc gcttcttcca
     1141 cgatcaacgg gtacgaatac cccgcgccga agtcgcgatt caccgtgacc acgccggtca
     1201 ccgtcacttg gtcgcccacc tgcacgatat cctgcgtggt gacggtcagg tcgttggccc
     1261 cggactttcc gctgccgtcc tggacgtgga agaagttctt gcccatgatc ccgttgttga
     1321 ccttgacgac cttcccgctg atctggatgc gctgtccgct gagcttatcc ttctgcgcga
     1381 atagggtttc gatgttcgag attttcgtgt cgcccgccat tgtcgggccc acggcgccga
     1441 gcatccacgc gagcaagagt cccacaccaa ataatgtgcg catcatcgcg atctcctgag
     1501 gttagagtaa atccggttgc cgttgtagca catttccggc tcgaaaaaaa gttacgcgct
     1561 gggggaagca ggcttggcgg tgaggcgcat caccaccacg tagaggacgc acaagagcaa
     1621 agcgagtccg gcgaaccacc agcctcggga ctggagcacg gcgatcatgt gcccgggatc
     1681 gtcgaggagt tgcggttggt cgccgatcac atgcgcgccg aaccacgcga gcaccgtgca
     1741 ccagagcgcg gaacccgcgg tggtcacgag caggtacagt ccgatcggca tgcgcacgat
     1801 gccggccgga atgccgatga gatgccggac caccgggagc aggcgagcga agaagacccc
     1861 gccggcctcg tagcgcgcca gccatcgctc cgcgcggacg agtttggcct cggtgacgcc
     1921 ggcgtagcgt ccgaagcgca cgatgagcgg ccgtcccacc gcgcgcgaca cccaataggt
     1981 gagtccggcg ccgatgacgc tgcccacggt gccggcgagc accacgcccc acaaggtgta
     2041 ccggccctgc gcggcccaat acgcggccgg aggaatcacg agttcgctgg gaaccgggaa
     2101 gatcgtgctc tccatggcca tgagcacgac gatgcccaga tagccccagt catggaccca
     2161 cgaaaaccag atggtaatga ggtgatgcac gatagactat ggggcgtgct cgcgaggcgt
     2221 ctcgtcgtga tggttgtccg cgggagacgg atcgggtacc gccgcgtccg gcgttccacg
     2281 atcgtgcggc gaagcgtcgt gcgggaccgt agtgtccggc atggtgtcgt gcgtccccgc
     2341 gtcgtgcgac atggctggcg cgtcgtggcc gctcccgtgc tcgtgatgat gcatctgggg
     2401 cggcgcaatg gccgcgtatg ctgtcagccc gatcatggat atcagagcga ttcccgccac
     2461 ggcgaggacg cctttgcgtc gagcagggtt gcgctcaggg cctcggtcta ccagcggcag
     2521 tacggccagg gccaggaaga gcgcgcccag ggcatgaaac atcgtgtcca tcttccccgt
     2581 gaggttttcc aatccgtaca gccacagaaa cggccacggg ggcttcagac tcatctcctc
     2641 cccggtgacc gggtcgccca ggggcgcggg cacgatcgca gccaacagcg cggttacgcc
     2701 gaagtagatc ccgccgaaga ggacgatgcc cttgacgtgt tcggtcatgg tgccggtcat
     2761 gcgttccttg ggcaccgact cgcgggccga gtcctcaccg aacggcaggg gcgacagact
     2821 gtgcgtgttg accaagtaaa agtgcgccgc aaggaacgcg acgatcacca gcggaaggat
     2881 cgagacgtgc gaggcataca gcttcacgtt gagcgacacc gcgtcggttg agcccaagaa
     2941 ggctcccagc ggccccagcc gggtgaggcc cgcctcgtag tgcttcaagg cgtcgaacga
     3001 ctcctgatcc cacttgagca cggtgcccga aaaatacgac ccgaacacgc caaggcccag
     3061 gagcgccaac cccacgtacc acgtgacaag gcgcggggct ttgtatgcgc cggagaacac
     3121 aatgcgcaac acgtgcagga tcagcgccac caccgcggcc tgcgcggtcc aatagtgcag
     3181 cgcgcgcacc cacgatccgc ctggcacgcg ctcgccgaga gctttgacgc tctggtacgc
     3241 ccgctccgga ctcggatcaa caaactgcgc gagcaagaac ccgctggcaa acaacagcag
     3301 aaagccgagg aacacgatgc cgcccaacgt gtacggcaat gtcttgccgt gcgcggggac
     3361 cggataggac aggcggctga gtttcagata tcgctcgatg atcccattcg ggcgatcaga
     3421 cggtcgtgat gacgggggac gcgcggcgcg ttgggccatg aatgcgggtg gctgggggac
     3481 taggattcga acctagatag gcagatccag agtctgcagt cctgccattg gacgatcccc
     3541 caaaacccgt ggcccgcgtg tacccagggc gaccggacgc ggtcggcgct gactctacgg
     3601 aaaacccggt ggggtgtcaa gacggcggtg cgaagcggcc ggacgcgatc gcgcggacgg
     3661 tttcgtcgat gcgggtcagg gcttcagact t
//
LOCUS       DBNGTT010000441         3684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3299295, whole genome shotgun sequence.
ACCESSION   DBNGTT010000441 DBNGTT010000000
VERSION     DBNGTT010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3299295"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..2216)
                     /locus_tag="AC16BC_09995"
     CDS             complement(48..2216)
                     /locus_tag="AC16BC_09995"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR3241992.1"
                     /translation="MPAVWLTRPAAGVAAWGLILLATSSVASAAEPVKPTLAAFPLAD
                     ARVVVDGVLDESVWRTVPGMSGLIQQTPRTGEPAVGVTTVWVAYDAQALYVAARLDDP
                     QPELIQADERHRDAAMARSDTFDVVIDTYHDHQNAFLFQTNPLGAQYDAALTREGAFV
                     NDAWNGIWEVAAGRTPTGWAAEFRIPFTTLRYHSSAGATWGIQFRRVIPHLMETSYWS
                     PLAPEQSLYEISRGGHLVGVAPLHPRRRLTLMPYAKGAYGVRSDDDAGWHVDHDAGGD
                     ARVAITPDLTLDLTLNTDFAETEADHFQVRLDRFPLFFPERREFFLEDKGAFEFGLEG
                     QVQPFFSRTIGLSGGEPVPLWGGAKLTGKADRYGIGALTMQSRARDNDPAERFSVLRL
                     TRDLGLRSQVGVIGTRREVLGGPPYQTVGADASYNPTSELTTNAFWMRSDGPQAAQPG
                     YAGYAQAQYRDPFWRLRLYHLRVDERFDPQLGFVKQNDLAETYGYVDVRPRPATGPVL
                     EWGVKTELTYQSTTDGTMLYRSQYQRVLAEFRTGDTILVSWDPQIERLQDSFELKPGI
                     VIPAGEYRYHHWNVYVASEPSRPFSGYVSVLWGGYYGGRKTSLDVNLTASAFETVKLG
                     AGLSVDWIRVPTGVAPYHADTVPRVLTGDVQWSVTNRLVLKGLVQHEHESGTLASYLR
                     LAWEYHPGSFVYLIANPSSSRETGSAAVYLIKVTWRWEAA"
     gene            complement(2226..2540)
                     /locus_tag="AC16BC_10000"
     CDS             complement(2226..2540)
                     /locus_tag="AC16BC_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241993.1"
                     /translation="MSNLSPEEAVIIVNAYGETLESLAVVSSPRWDSDEAYQRDVMSS
                     PDPMALLRRTYDLRLLPYSKNAIRDALHVLLAHPDVDADMKNRLRMGLLALDDFVEGA
                     EA"
     gene            2695..3468
                     /locus_tag="AC16BC_10005"
     CDS             2695..3468
                     /locus_tag="AC16BC_10005"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR3241994.1"
                     /translation="MSRIARLALVTVVAMAWTPAFGAPNVPFAHGERLVYSIGWYNVN
                     GGIATLDLDEVDYHGVPVYRIRSLAKSNAVVSVFFPVEDRIESVIDRDTLSALHLDVK
                     QRQGKRKRDRVTEFDQVNHTATVFKNGEQKVYEIPPGVQDSLSCLYYFRSLPPIRVGE
                     TVTIDVHESNKNWRLAIVALNREKVRTSAGEFMAIRTRAKVEFEGVFLERGEVYVWFT
                     DDDRRLPVRMESKIKVGRISAKLIEYRPGAKPADEARAP"
     gene            3465..>3684
                     /locus_tag="AC16BC_10010"
     CDS             3465..>3684
                     /locus_tag="AC16BC_10010"
                     /inference="COORDINATES: protein motif:HMM:NF015821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR3241995.1"
                     /translation="MSSAPLCPICRTRKADPAHTPFCSARCRDVDMGRWLSGAYRVPT
                     EEPASSETAESSEPSGAPDKANPSNPAKP"
BASE COUNT          610 a         1209 c         1242 g          623 t
ORIGIN      
        1 tgcaccccat cgccccctct cccgctctcc ccctcgtgcc ttccccccta cgccgcttcc
       61 caccgccacg tgactttgat cagatacacc gcggccgacc cggtctcgcg cgaggacgac
      121 gggttcgcga tcagatacac gaaactcccc ggatgatact cccacgcgag gcgcaggtag
      181 gacgcgagcg tgccgctctc gtgttcgtgt tgcaccaagc ccttgagcac cagacggttg
      241 gtcacggacc attgcacatc gccggtgagg acgcggggca cggtgtcggc gtgatagggc
      301 gcgacgccgg tggggacgcg gatccagtcc acgctcaacc cggcgcccag tttgacggtc
      361 tcgaacgccg acgcggtcag gttcacgtcc agcgaggtct tcctgccccc gtagtatccg
      421 ccccacaaca cgctcacgta cccggagaat ggacggctcg gctcgctcgc cacgtacacg
      481 ttccagtgat ggtagcggta ttcgccggct gggatgacga tcccgggttt cagctcaaac
      541 gaatcctgga gccgctcgat ctgcgggtcc caactcacca ggatcgtgtc cccggttcga
      601 aactctgcga gcacgcgttg gtactggctg cggtacagca tcgtgccgtc ggtggtgctc
      661 tggtaggtca gttcggtttt gaccccccat tccaggaccg ggccggtggc gggcctcggg
      721 cgcacgtcga cgtacccgta cgtttccgcc aggtcgttct gtttcacgaa tcccagttgc
      781 ggatcaaagc gctcgtccac gcgcagatgg tacaaccgga gccgccagaa cgggtcgcgg
      841 tactgggcct gggcgtaccc cgcgtagccg ggttgcgcgg cttgcggccc gtccgagcgc
      901 atccaaaacg cgttggtggt gagctccgac gtgggattgt acgaggcgtc cgcgcccacg
      961 gtctggtacg gcgggccgcc cagcacttcg cgtcgcgtgc cgatcacgcc cacctgcgag
     1021 cgcaggccca ggtcgcgggt caggcgcagg acgctgaagc gctccgcggg atcattgtcg
     1081 cgcgcgcggc tttgcatggt gagcgcgccg atgccgtagc ggtcggcttt acccgtgagt
     1141 ttggcgccgc cccagagcgg aaccggctcg cccccgctca acccgatggt ccggctgaaa
     1201 aacggctgca cctggccttc caggccgaac tcgaacgcgc ccttgtcttc gaggaaaaac
     1261 tcgcggcgtt ccgggaagaa caggggaaag cggtcgagcc gcacctggaa atggtccgct
     1321 tcggtttccg cgaaatccgt gttcagggtc aggtcgaggg tgagatccgg ggtgatcgcg
     1381 acccgcgcgt cgcctcccgc gtcgtggtcc acgtgccagc cggcgtcgtc gtcagatcgc
     1441 acgccgtacg cgcccttggc gtacggcatg agggtgaggc gtcgtcgggg gtgcagcggc
     1501 gccacgccca ccaggtgccc gccgcgcgag atctcgtaca acgactgttc cggggcgagc
     1561 gggctccagt acgaggtctc catcaggtgg gggatcacgc ggcgaaactg gatgccccac
     1621 gtggcgcccg cggacgaatg gtagcgcagg gtcgtaaatg gaatgcgaaa ttccgcggcc
     1681 catcccgtgg gagtgcggcc ggccgccacc tcccagatcc cgttccacgc gtcgttgacg
     1741 aacgcgcctt cgcgggtcag ggccgcgtcg tactgcgcgc ccaagggatt ggtctgaaac
     1801 agaaacgcgt tctggtgatc gtgatacgtg tcgatcacca cgtcgaacgt atcgctgcgg
     1861 gccatggcgg cgtctcggtg gcgctcgtcg gcctggatca attccggttg ggggtcgtcg
     1921 agccgcgccg ccacgtagag cgcctgcgcg tcgtacgcca cccagacggt ggtgacgccg
     1981 accgccggtt cgccggtgcg cggcgtttgt tgaatcaatc cgctcatgcc ggggaccgtc
     2041 cgccagacgg actcatcgag caccccgtcc acgaccacgc gcgcgtccgc gagcgggaac
     2101 gcggcgagcg tgggcttgac cggctcggcg gccgacgcga cgctcgacgt cgcgagcagg
     2161 atcagccccc atgcggcgac gccggcggcc ggccgcgtca gccagacggc cggcatgtgc
     2221 cggcgctacg cctcggcgcc ttcgacgaag tcgtcgaggg cgagcaaccc catgcgcagc
     2281 cggttcttca tgtcggcgtc aacgtccggg tgcgcgagga gcacgtgcaa ggcgtcgcgg
     2341 atggcgttct tcgagtaggg caggaggcga aggtcgtacg tgcggcgcag cagcgccatg
     2401 ggatccgggc tgctcatcac gtcgcgctga taggcttcgt cgctgtccca gcgcggggac
     2461 gacaccacgg ccagggattc gagcgtctcg ccgtaggcgt tgacgatgat gacggcttct
     2521 tccgggctca gattggacat ggggcgacga ccgggatcac ggacggcacc atatcacgat
     2581 cctcggtcca cccgcaaatc gacggcgaag ccccatgccg atcgcggacc gtccttgtca
     2641 aggcccctga ccgtttctat aatcacgcgc atgaggcgtg ttcgacacgg gcccgtgtct
     2701 cggatcgcgc ggcttgcgct cgtgaccgtc gtggcgatgg catggacgcc ggcgttcggc
     2761 gcgccgaacg tgccctttgc tcacggcgaa cgcctggtgt actcgatcgg gtggtacaat
     2821 gtgaacggcg gcatcgcgac gctggacctg gacgaggtcg actaccatgg agttccggtg
     2881 tatcgcatcc gatcgctggc caaatccaac gcggtcgtgt ccgtcttttt cccggtggaa
     2941 gatcgcatcg aaagcgtgat cgaccgcgac acgctgagcg cgttgcatct ggacgtcaag
     3001 cagcgacaag gcaaacggaa acgggaccgg gtcacggaat tcgatcaggt caaccacacc
     3061 gcgaccgtgt tcaagaacgg cgagcaaaag gtgtacgaga ttccgcccgg cgtacaggac
     3121 tcgttgtcgt gtctatacta ctttcggagc cttccgccca ttcgggtcgg cgagaccgtg
     3181 accatcgacg tgcatgagag caacaagaac tggcggttgg cgatcgtggc gctgaaccgg
     3241 gaaaaggttc ggacctccgc cggcgagttt atggccatcc ggaccagggc caaggtggag
     3301 ttcgagggcg tcttcctgga acgcggggag gtctacgtct ggttcaccga cgacgatcgg
     3361 cggctcccgg tgcgcatgga gagcaaaatc aaggtcgggc ggatctcggc gaagctcatc
     3421 gaatatcgcc ccggcgccaa gccggcggac gaggctcgcg cgccgtgagt tccgccccgc
     3481 tgtgcccgat ctgtcgaacg cggaaggccg atcccgcgca cacgccgttt tgcagcgcgc
     3541 ggtgccgcga cgtggacatg ggccggtggc tctccggcgc gtatcgcgtg ccgaccgagg
     3601 agcccgcgtc gtcggagacg gccgaatcct cggaaccgtc cggggcgccg gataaggcga
     3661 atccgagtaa tcctgcgaag cctt
//
LOCUS       DBNGTT010000442         2910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2150054, whole genome shotgun sequence.
ACCESSION   DBNGTT010000442 DBNGTT010000000
VERSION     DBNGTT010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2150054"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..175)
                     /locus_tag="AC16BC_10015"
     CDS             complement(2..175)
                     /locus_tag="AC16BC_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241996.1"
                     /translation="MFDELTQLQGREVEVVYEGIIYRGRLVGADESELYLLTPGGSFN
                     LPLENITSVRPAA"
     gene            330..554
                     /locus_tag="AC16BC_10020"
     CDS             330..554
                     /locus_tag="AC16BC_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241997.1"
                     /translation="MAQSVDLTKAITQAKQQVKERRAAVESPRQDTALRGLRKTLRRL
                     QRRRRAATAQQAQLKAKATKKSGGEAAKVG"
     gene            561..1382
                     /locus_tag="AC16BC_10025"
     CDS             561..1382
                     /locus_tag="AC16BC_10025"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3241998.1"
                     /translation="MASLGLALGGGGVRGFAHLGVLRVLEREGLAPTAIAGTSMGAII
                     AALYALSPSLNAAEHRLGEFFLRFGPVLGHLAERVASSRVGIAVDEPSLLDAGVFHQH
                     FDSLIPDTTFDKTRLPLAVVAVDLVSGEEVVFTSGPVRPAVLGSLALPGVFPPIGYDG
                     RVLVDGGWTSRIPVEAVRQLGAERVLAIEVSDNVETPFPINQPTSGLQIVLRAAEITR
                     NHLARYAARNADLLLVPPTGQIALHDASRASECVRLGAEATQARITEIRALVTGR"
     gene            complement(1413..1868)
                     /locus_tag="AC16BC_10030"
     CDS             complement(1413..1868)
                     /locus_tag="AC16BC_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3241999.1"
                     /translation="MGGILKALLFAGCLALAFAAGYWLGDHRLDDVTSHVTSLREDLG
                     EKTKAIDRQMLALKRRETLAQAREALARAREALAEKNFGEAEDEIAAAAERIDALARD
                     AAAGTKTALTSVGEALKDVRKKVRASQEGVRDQLRRLARRLDELARNWE"
     gene            2011..2703
                     /locus_tag="AC16BC_10035"
     CDS             2011..2703
                     /locus_tag="AC16BC_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3242000.1"
                     /translation="MTVRSVMTRRASFALALAAIVGMTACAAHEDPALKPLVALSDLQ
                     YDAGVIISKAENLYDEQKWDEAAVEYERFLELHPVHRWAPHAQFKLALCYTQRIPAVG
                     RDPSIAEKAEAAFGRVLSYPDTRYADVARAKLAEVRTHRAKSDVEVGRFYLKQHKYPA
                     AITRFQNVIATKLGGQVTEDASYYLAVAYERDGQREQATQAAQDLMDAFPQTRYAKSV
                     AKLQARLASHVD"
     gene            2693..>2910
                     /locus_tag="AC16BC_10040"
     CDS             2693..>2910
                     /locus_tag="AC16BC_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3242001.1"
                     /translation="MSTDPRYYPAVLNLRDRAVVVVGGGVIATRKVAELLTTGARVTV
                     ISPTVSPRLARWAREGRLRHRARPYRRG"
BASE COUNT          475 a         1041 c          909 g          485 t
ORIGIN      
        1 gctacgcggc cgggcgcacc gaggtgatgt tttccagcgg aaggttgaac gaaccgccgg
       61 gcgtcagcaa atacagctcg gattcgtcgg cgccgacgag tcggccgcgg tagatgatgc
      121 cctcgtaaac gacctcgact tcccggcctt gcagttgggt cagttcgtcg aacaccgtcg
      181 cctctgtctc ggattatatc gacaagccga cggccgcgca acgcgacccc gcgattccga
      241 gcgtggggtc ctggaggcga tgcgtcgacg ctccccgttg cctcgcccca ggaggtatgg
      301 taggctcccg ccgatcactg gagacaccca tggctcaatc ggttgattta acaaaggcca
      361 ttacccaagc aaagcagcag gtcaaggagc ggcgcgccgc ggtcgagtcg ccgcggcagg
      421 acaccgccct ccgcggcctc cgcaagacct tgcgtcggct gcaacgccgt cggcgcgccg
      481 ccaccgcgca acaggcgcag ctcaaagcca aggccaccaa gaaatccggc ggggaggccg
      541 cgaaagtcgg gtaacggccg atggcctcgc tcggactcgc cctgggcggc gggggcgttc
      601 gcggcttcgc gcacctcggg gtcctgcgcg tgctggagcg cgaaggcctc gcgcctaccg
      661 cgatcgcggg gaccagcatg ggggcgatca tcgccgccct gtacgcgctc tcgccctccc
      721 tcaacgccgc ggagcaccgc ctcggcgaat tcttcctccg gtttgggccg gtcttgggtc
      781 acctcgcgga gcgcgtggcg tccagccgcg tcggcatcgc cgttgatgag ccgtccttgc
      841 tggacgccgg cgtgttccac caacacttcg acagcctgat tcccgacacc acgttcgaca
      901 agacccgcct tcccttggcc gtcgtcgccg tggacctggt ctccggcgaa gaggtcgtgt
      961 tcacctccgg gccggttcgg cccgcggtgt tgggcagcct cgcgctgccc ggagtgtttc
     1021 cgccgatcgg gtatgacgga cgcgtcctcg tggacggcgg ctggaccagc cgtatcccgg
     1081 tcgaggcggt tcgccaactc ggggcggagc gggtcctcgc gatagaagtc tcggacaacg
     1141 tcgaaacgcc gtttccgatc aatcaaccca cgagcggcct gcaaatcgtg ctgcgcgccg
     1201 ccgaaatcac gcggaaccat ctggcccggt acgccgcgcg caacgccgat ttgctgcttg
     1261 ttcctccgac cgggcagatc gcgctgcacg acgcgtcgcg cgcgtcggag tgcgtccggc
     1321 tcggcgcgga ggcgactcag gcgcggatca cggagattcg cgcgctggtc accggccggt
     1381 aacgcgacgc cccagcacat acagggccgc ccctactccc agttccgggc gagttcatcg
     1441 agtcgtctcg ccaggcgacg caactgatcc cgaacaccct cttgtgacgc gcggaccttc
     1501 ttccgcacgt ctttgagggc ctcgccgaca gacgtcagcg ccgtcttggt ccccgccgcg
     1561 gcatcccgcg cgagcgcgtc gatccgctcc gccgcggccg cgatttcatc ttccgcttct
     1621 ccgaaatttt tctccgccag cgcttcgcgc gcgcgcgcca acgcctcccg cgcctgcgcc
     1681 aacgtttccc gacgcttgag cgccagcatc tgccgatcaa tcgccttcgt cttctcgccg
     1741 agatcttccc tcagcgacgt gacgtgcgac gtaacgtcgt cgagccggtg atcccccagc
     1801 caataccccg ccgcgaacgc cagcgccaga catccagcaa acaacaacgc cttcaagatt
     1861 ccacccatcc ctgttctcca cgcatccgtt gatcgtgact gagtcgccgc tggcccggcc
     1921 ggcgcgacgt atgttccccc gcgccccccg cccgagtcaa gcgcgtgacc ttgattttca
     1981 cgtgacggcc ctgctagtct cgcgccgctt atgactgtcc gttccgtgat gacgcgtcgt
     2041 gcttcattcg ccctcgcgtt ggccgcgatc gtcgggatga ccgcgtgcgc cgcccacgag
     2101 gaccccgcgc tcaaaccgct cgtcgcgctc agcgacctcc agtacgacgc gggcgtcatc
     2161 atcagcaagg ccgaaaacct ctacgacgaa cagaagtggg acgaggccgc ggtcgaatac
     2221 gagcggttcc tggagcttca tccggttcat cgatgggcgc cccatgccca gttcaagctg
     2281 gcgctctgtt acacccaacg cattcccgcg gtcggcaggg atccatcgat cgctgaaaag
     2341 gccgaagccg cctttggacg ggtgctgtcg tatccggaca ccaggtacgc ggatgtcgcg
     2401 cgcgctaaac tggccgaggt gcggacgcac cgggcaaaat cggatgtcga ggtcggacgg
     2461 ttctacctga aacaacacaa gtatcccgcc gcgatcaccc gtttccagaa cgtgatcgcc
     2521 accaagctcg gcgggcaggt gacggaagac gcgtcgtact acctggcggt ggcctatgaa
     2581 cgcgacggac aacgggagca ggccacgcag gccgcgcagg atctgatgga cgcctttccc
     2641 cagacgcggt acgcgaagtc ggtggcgaaa ctccaggcgc gtctcgcgtc tcatgtcgac
     2701 tgatccgcgc tactatccgg cggtgctgaa tctccgtgac cgcgccgtgg tggtggtcgg
     2761 cgggggcgtc atcgcgacgc gcaaagtcgc cgagctgctg acaaccggcg cccgcgtgac
     2821 ggtcatcagt ccaaccgtca gcccccggct ggcgcgctgg gcccgtgaag gacggctgcg
     2881 gcaccgagcg cgcccctacc gccgcggcga
//
LOCUS       DBNGTT010000443         6423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2022398, whole genome shotgun sequence.
ACCESSION   DBNGTT010000443 DBNGTT010000000
VERSION     DBNGTT010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2022398"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="sucC"
                     /locus_tag="AC16BC_10045"
     CDS             <1..867
                     /gene="sucC"
                     /locus_tag="AC16BC_10045"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR3242002.1"
                     /translation="SIGHEFYLSLVVDRASASIAVVASREGGMEIEEVAAKHPEKIIK
                     VFIDPTVGYQPFHGRQVAFGLRLPKEVVNGFVKLLGSLYALFMAKDASLVEINPLVLT
                     TDQKFVALDGKMNFDDNGLFRQEDVRAFRDLDEEEPLEIKASEHNLNYVKLDGTIGCM
                     VNGAGLAMATMDVVKLAGGEPANFLDVGGGATKETVKEAFNILMADPNVKGVFVNIFG
                     GIVRCERIAGGIIDATREVNLRVPLVVRLDGTNAEEGKKMLASSGLNLDVAGTMWEGA
                     QRIVSLSGEAVR"
     gene            864..1895
                     /gene="sucD"
                     /locus_tag="AC16BC_10050"
     CDS             864..1895
                     /gene="sucD"
                     /locus_tag="AC16BC_10050"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3242003.1"
                     /translation="MSVLVDKDSRVVVQGLTGREGGFHADQCLAYGTKVVGGVTPGKG
                     GATWVGETSGKKAPVYDTVRQAVNDAGANVSLIFVPPPFAADAILESVDAEVPLIICI
                     TEGIPVLDMMRVKRALAGKSSRLIGPNCPGVITPGEAKIGIMPGFIHRPGSVGVVSRS
                     GTLTYEAVWQVTQRGLGQSTCVGIGGDPLNGTNFIDCLDLFNRDPDTEAIVMIGEIGG
                     DAEEQAAAFIARSVKKPVVGFIAGITAPPGRRMGHAGAIISGGVGSAADKMSALEEAG
                     VIVVKNPALIGEAIKAALGRRTRKAPAKAKAPAKPKARPARRATTRTRSVAAPRSRAR
                     GRGRGRGTR"
     gene            1892..2470
                     /locus_tag="AC16BC_10055"
     CDS             1892..2470
                     /locus_tag="AC16BC_10055"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3242004.1"
                     /translation="MKMTTVVFTLTAILVIGAGWLWATSGRAEMAVPVSIGQMAPDFT
                     LNDQNGMPVTLSDFWGRQTVVLYFYPKDNTPGCTKEACSFRDDAARFQALNARVLGVS
                     VDGLESHRGFAEKYKLTFPILSDEDHRVSRAYGVLTEFAGFAFAKRTTFVIGSDGVIR
                     RIFTDVDPKDHSAELLQYLKSISVSAESSTEG"
     gene            2474..3643
                     /locus_tag="AC16BC_10060"
     CDS             2474..3643
                     /locus_tag="AC16BC_10060"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR3242005.1"
                     /translation="MYVIGLSSGTSVDGIDAALTEVTGRGSRAKIRLITYRTLAYPRG
                     VRERVLALQSDSTDTLVELCRLNAYLGELFADAAIKLAKAAGVPLRRVGLIGSHGQTV
                     AHQPAPVREGRFRVAGTLQIGEPSVIAERTGVTTVADFRPRDLAAGGQGAPLTALIHH
                     RCFAHARRDRLVVNLGGITNVTWIPAGHVADPSAITAFDTGPGNMLIDGLADRFTRGR
                     ETMDRGGRMAASGAVHDRLLASLLTHPFLAKRPPKTTGRETFGRALADRVAADARRLR
                     VAPRDVMATVTAFTARTIADAHARFLRSRARNPALDVIVGGGGVFNATLMAELRRAFP
                     RAGVESMAAHGVDPKAIEAMAFALLAYLTFHREPDNVPSATGANRAVVLGKIIPK"
     gene            3762..6191
                     /locus_tag="AC16BC_10065"
     CDS             3762..6191
                     /locus_tag="AC16BC_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3242006.1"
                     /translation="MFERFTDRGRKIIILAREEAERHQNDYLGTEHLVLAILREGDGV
                     ALAVIKKMGLSPEQVRLEIERNLPSGGSTMTFGEIPFTPRVKKVIEFSIEEAKLLGHN
                     YIGSEHLLLGLLREEEGMGGKILRSLGANLLTARQLTINLLKKSVPREKDKKSNTPAL
                     DEFGRDLTQLATEGSLDPVIGRADEIERVLQILCRRTKNNPVLIGESGVGKTAIVEGL
                     AQRIIAADVPDNLLNRRVIALDLGSLVAGTKYRGQFEERLKVVMKEIVSAGNIIIFID
                     ELHTLVGAGAAEGSIDASNMLKPALARGEIQCIGATTLDEYRKHIERDAALKRRFQPI
                     HVNPPSTDETIKIIRGLKDRYEDHHGITITEDAVVEAVRLSDRYITDRFLPDKAIDVI
                     DEAGSRAKLLNYALPADLRGLEQEHKRVAKEKDVAIKAQNFEEAVKLREEEERLKKML
                     EDSKREWKRNQEKQRTNITKEEVALVVSKMTGIPLYKLEEEESKKLLQMEEALHKRVI
                     GQEEAIAAVSKAIRRARAGLKGEHRPIGSFIFLGPTGVGKTELARTLAEFLFDDEDAL
                     IRIDMSEYMERFASSRLVGAPPGYVGYEEGGQLTEKVRRRPYSVVLFDEIEKAHPEIF
                     NMMLQVLDDGALTDSLGRKVDFKNTVIIMTSNVGARMIDKGASLGFRQESEHGEGRRI
                     KDDVMGELKKTFNPEFLNRIDEIVVFHTLTKEHLVKIVDILIDGVNRRLVEKGIQIEL
                     TEDVKKWLIEEGYQPLYGARPMRRCVQRHIEDPLSEELLRGRFKDQKRIKVVLRDNAP
                     TFSEEPAMAGV"
     gene            6281..>6423
                     /locus_tag="AC16BC_10070"
     CDS             6281..>6423
                     /locus_tag="AC16BC_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3242007.1"
                     /translation="MLILGIETSCDETAAAIVRDGSAIVSEELATQTGVHSKFGGVVP
                     ELA"
BASE COUNT         1212 a         1975 c         2227 g         1009 t
ORIGIN      
        1 agcatcggcc acgagttcta tctgtcgctg gtggtggacc gcgcgtccgc gtcgatcgcg
       61 gtggtggcga gccgcgaggg cgggatggaa atcgaagaag tcgcggccaa acacccggaa
      121 aaaatcatca aggtgttcat cgatcccacc gtggggtacc agccgtttca cggccggcag
      181 gtggcgttcg gcctgcgcct gcccaaggaa gtggtcaacg ggttcgtgaa actgctcggt
      241 tcgttgtacg cgttgttcat ggccaaggac gcgtcgttgg tcgagatcaa cccgttggtg
      301 ctgaccacgg atcagaagtt cgtggcgctg gacgggaaga tgaacttcga cgacaacggc
      361 ttgttccgcc aggaggacgt gcgcgcgttc cgcgacctcg acgaggaaga gccgctggaa
      421 atcaaggcgt cggagcacaa cctcaattac gtgaaactcg acggcacgat cggctgcatg
      481 gtcaacggcg cgggcctcgc catggcgacc atggacgtgg tcaaactcgc cgggggcgaa
      541 cccgcgaact tcctggacgt gggcggcggc gccaccaagg aaaccgtgaa agaggcgttc
      601 aacatcctga tggcggaccc gaacgtgaag ggcgtgttcg tgaacatctt cggcggcatc
      661 gtgcgctgcg aacggatcgc gggcgggatc atcgacgcca cgcgcgaagt caatctcaga
      721 gtgccgttgg tcgtccgatt ggacgggacc aacgcggaag agggcaagaa gatgctggcc
      781 tcgtcggggc tcaatctcga tgtggccggc acgatgtggg agggggctca gcggatcgtc
      841 tccctttccg gggaggccgt gcgatgagcg ttctggtcga taaggattcg cgcgtggtgg
      901 tgcaggggct caccggccgc gaggggggat ttcacgcgga ccagtgcctg gcctacggga
      961 ccaaggtcgt gggcggggtg acgccgggca agggcggcgc cacgtgggtc ggggagacct
     1021 cgggcaagaa ggcgccggtc tacgacaccg tgcgccaggc cgtgaacgac gcgggcgcga
     1081 acgtgtcctt gatcttcgtg ccgccgccgt tcgcggcgga cgcgatcctg gagtcggtgg
     1141 acgccgaggt gccgctgatc atctgcatca ccgagggcat tccggttctc gacatgatgc
     1201 gggtcaagcg cgcgctggcc ggaaaatcct cgcggttgat cggcccgaac tgtcccgggg
     1261 tcatcacgcc cggcgaggcc aagatcggga tcatgccggg gttcattcac cgccccgggt
     1321 cggtcggcgt ggtgtcgcgc agcgggaccc tgacgtatga agcggtgtgg caggtcacgc
     1381 aacgcggcct gggccagagc acgtgcgtgg gcataggcgg cgatccgctc aacggcacca
     1441 acttcatcga ttgcctcgac ctgttcaatc gcgatcccga caccgaggcg atcgtgatga
     1501 tcggcgagat cggcggcgac gccgaggagc aggccgcggc gttcatcgcg cggtcggtga
     1561 agaaacccgt ggtcggattc atcgcgggca tcaccgcgcc gccgggacgt cgcatgggcc
     1621 acgcgggcgc gatcatttcc ggcggggtcg gcagcgcggc ggacaagatg tcggctctgg
     1681 aagaggccgg cgtgatcgtg gtgaaaaacc cggcgttgat cggcgaggcc atcaaggccg
     1741 cgctgggacg gcgcacgcgc aaggctccgg ccaaagccaa ggccccggcc aagcccaagg
     1801 cccgcccggc gcgtcgggcc acgacgcgca cgcgatccgt cgcggccccg cgctcacgcg
     1861 cgcggggccg ggggcgggga aggggcacgc gatgaaaatg accaccgtcg tcttcacgct
     1921 caccgcgatc ctcgtcatcg gggccgggtg gctgtgggcc acgtcaggac gcgcggagat
     1981 ggcggtgccg gtgagcatcg gccagatggc gccggacttc acgctcaacg accagaacgg
     2041 catgccggtg acgctcagcg acttctgggg gcggcagacc gtggtgctgt atttctaccc
     2101 caaggacaac acgccgggat gcacgaaaga agcgtgcagt ttccgcgacg acgcggcccg
     2161 gttccaggcc ctcaacgctc gggtgctggg cgtgagcgtg gacgggctgg agtcccatcg
     2221 cgggttcgcg gaaaaataca agttgacctt cccgatcctc agcgacgagg accaccgggt
     2281 cagccgcgcc tacggcgtgt tgaccgagtt cgcgggattc gccttcgcca agcgcaccac
     2341 gttcgtgatc ggctcggacg gcgtaatccg ccggatcttc accgacgtgg accccaagga
     2401 ccattcggcc gaactcctgc agtatctcaa gagcatcagc gtatcggctg agtcctcgac
     2461 cgaaggctag ttcgtgtacg tgatcggtct ctcgtcgggc acttccgtcg atgggatcga
     2521 tgcggcgctc accgaagtca cggggcgcgg atcacgcgcc aagattcgcc tgatcacgta
     2581 ccgcaccctg gcctatccgc gcggggtgcg cgagcgcgtg ctggcgctgc aatccgattc
     2641 aaccgatacc ctggtcgagc tctgccggct caacgcgtat ctgggcgaac tgttcgcgga
     2701 tgccgcgatc aagctggcca aggccgcggg cgtgccgctg cgccgggtgg gcttgatcgg
     2761 gtcgcacggc cagaccgtgg cgcatcaacc cgcgccggtc cgggaagggc gatttcgcgt
     2821 cgccggcacg ttgcagatcg gcgagccgtc cgtcatcgcg gaacgcaccg gggtgaccac
     2881 ggtcgcggat tttcggccgc gtgatctcgc ggcgggcggg cagggcgccc cgctcaccgc
     2941 gctcatccat catcgctgtt tcgcccatgc ccgtcgcgat cggttggtgg tcaatctggg
     3001 cggcatcacc aacgtcacgt ggattccggc cggccatgtc gcggatccga gcgcgatcac
     3061 ggcgttcgac accggacccg gcaacatgct catcgacggg ctcgcggacc ggttcacgcg
     3121 cgggcgcgag accatggatc gcggcggccg catggcggcg tccggcgcgg tgcacgaccg
     3181 gttgctggcc tcgttgctca cacacccgtt cctggcgaaa cggccgccca agaccaccgg
     3241 gcgggaaacg ttcggccgcg ccttggcgga ccgcgtcgcg gccgacgcgc gccggctgcg
     3301 cgtggcgccg cgcgacgtga tggccacggt caccgcgttc accgcgcgca cgatcgcgga
     3361 cgcgcacgcg cgatttctgc gctcccgcgc gcgcaacccc gcgttggacg tgatcgtggg
     3421 cggcggcggg gtgttcaacg cgacgttgat ggccgagttg cggcgcgcgt tcccccgtgc
     3481 cggggtggaa tccatggccg cgcacggcgt ggaccccaag gcgatcgagg ccatggcgtt
     3541 cgcgttgctc gcgtatctga cgtttcaccg cgaacccgac aacgtgccct cggccacggg
     3601 cgcgaaccgc gccgtcgtcc tcgggaaaat tatcccgaaa tagaatgcct tttcggccaa
     3661 aaatcggcgc gttgcttgac atggggttgc ctcaccgtta gaatgaaaac gaaccgtccg
     3721 gccaggtttt cagcggccgg gttgatcctc aggaggcccg aatgttcgag cgattcaccg
     3781 atcgaggcag gaaaatcatc atcctcgcga gggaggaagc cgagcgtcac cagaacgact
     3841 atctcggcac cgaacacctt gtgctcgcga tccttcgcga aggcgacggc gttgcgctgg
     3901 ccgtgatcaa gaaaatgggg ttgtcgccgg agcaggtgcg tctcgagatc gagcgcaatc
     3961 tgccgagcgg gggcagcacc atgacgttcg gggagattcc gttcaccccc cgcgtgaaga
     4021 aagtcattga attttcgatc gaagaagcca aactgctcgg ccacaattat atcggcagcg
     4081 agcacttgct gttggggttg ttgcgcgagg aagagggcat gggcgggaag atcctccgca
     4141 gcctgggggc caacctgctg accgcgcggc aactgaccat caacctgctc aagaagagcg
     4201 ttccgcgcga gaaggacaag aagagcaaca cccccgcgct cgacgaattc ggccgcgatc
     4261 tcacgcagtt ggcgaccgaa ggctcgctgg acccggtgat cgggcgcgcg gatgaaatcg
     4321 aacgcgtgtt gcagatcctc tgccgtcgga ccaagaacaa cccggtcctg atcggcgaat
     4381 ccggcgtggg caaaaccgcg atcgtggagg gcctggcgca gcgcatcatc gcggcggacg
     4441 taccggacaa cctcctgaac cggcgcgtga tcgcgctgga cctgggttcg ctggtggcgg
     4501 gcacgaaata ccgcgggcag ttcgaggagc ggctcaaggt cgtgatgaag gagatcgtga
     4561 gcgccggcaa catcatcatc ttcatcgacg agctgcacac cttggtcgga gccggcgcgg
     4621 ccgagggatc catcgacgcg tccaacatgc tcaagcccgc gctcgcgcgg ggcgagatcc
     4681 agtgcatcgg cgccacgacc ctcgacgagt accgcaagca catcgagcgg gacgcggcgc
     4741 tcaagcggcg gttccagccg atccacgtga atccgccgag cacggacgag accatcaaga
     4801 tcatccgggg cttgaaggac cggtacgaag atcaccacgg catcaccatc acggaagacg
     4861 ccgtggtgga ggcggtgcgc ctctccgacc gctacatcac ggaccggttc ctgccggaca
     4921 aggcgatcga cgtgatcgac gaggcgggct cgcgcgccaa actgctcaac tacgcgttgc
     4981 cggccgacct gcgcgggttg gagcaggagc acaagcgggt ggcgaaggaa aaagacgtcg
     5041 ccatcaaggc gcagaacttc gaagaagcgg tcaagctccg cgaggaggaa gagcgcctca
     5101 aaaagatgct cgaagactcc aagcgggaat ggaagcgcaa tcaagagaaa cagcggacca
     5161 acatcaccaa ggaggaagtg gccctggtgg tgtccaagat gaccgggatt cccctctata
     5221 aattggagga agaggagtcc aagaagctcc tgcagatgga agaggcgctg cacaaacgcg
     5281 tgatcgggca ggaagaggcg atcgcggccg tgtccaaggc catccgtcgg gcgcgcgccg
     5341 ggctcaaggg cgagcaccgt ccgatcgggt cgttcatctt cctgggcccc accggcgtgg
     5401 gcaagaccga attggcccgc accctggcgg agttcctgtt cgacgacgag gacgcgctga
     5461 tccggatcga catgtccgag tacatggagc gcttcgccag ctcgcggctg gtgggggcgc
     5521 ctccgggata cgtggggtac gaggaaggcg gccagttgac cgagaaggtc cgtcgccggc
     5581 cgtactcggt ggtgctgttc gacgagatcg aaaaggcgca tccggaaatc ttcaacatga
     5641 tgctccaggt cctggacgac ggcgcgttga ccgacagcct cggccgcaag gtggacttca
     5701 agaacacggt catcatcatg acgtccaacg tgggcgcgcg catgatcgac aaaggcgcgt
     5761 cgctcggatt ccggcaggaa tccgagcacg gcgaaggccg ccggatcaag gacgacgtga
     5821 tgggcgaact gaagaagacg ttcaacccgg aatttctcaa ccggatcgac gagatcgtgg
     5881 tcttccacac gttgaccaag gagcacctgg tcaagatcgt ggacatcctc atcgacggcg
     5941 tgaaccggcg gctggtggag aaggggattc agatcgagct caccgaagac gtgaagaagt
     6001 ggctgatcga ggaaggctat caacccttgt acggcgcgag gccgatgcgg cgctgcgtgc
     6061 agcggcacat cgaagatccc ttgtccgagg agttgctgcg cgggcggttc aaggaccaga
     6121 agcgcatcaa ggtggtgctc cgcgacaacg cgcccacgtt cagcgaagag cccgcaatgg
     6181 ccggcgtgta acgcgcccgg tccgacgaat catggtggag gggcggggat gacccgcccc
     6241 tccgtgtttg tgcttcccgc ggttcctgcc atttgacgac gtgctgatcc tgggaatcga
     6301 aacctcctgc gacgaaaccg ccgcggccat cgtgcgcgac gggtcggcca tcgtgtccga
     6361 ggagttggcc acccagaccg gcgtgcactc caagttcggc ggggtggtcc cggagctggc
     6421 gtg
//
LOCUS       DBNGTT010000444         2970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_999972, whole genome shotgun sequence.
ACCESSION   DBNGTT010000444 DBNGTT010000000
VERSION     DBNGTT010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_999972"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="AC16BC_10075"
     CDS             <1..403
                     /locus_tag="AC16BC_10075"
                     /inference="COORDINATES: protein motif:HMM:NF017555.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HIR3242008.1"
                     /translation="AFRRLAALTAFSQRPAFESLMTACKRVMNILPEPCPAAIDPARF
                     THDAERRLHAETQRAAAAVAVHEEQGRDEAVLETFESLKPAIDGFFTDVMVMADDPAV
                     RDTRLALLKAVNDLLARFADFRAVATTETAR"
     gene            488..>2970
                     /gene="ppdK"
                     /locus_tag="AC16BC_10080"
     CDS             488..>2970
                     /gene="ppdK"
                     /locus_tag="AC16BC_10080"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR3242009.1"
                     /translation="MARSATHKWVYSFGDGRAEGKAGMKDLLGGKGAGLAEMTNLKIP
                     VPPGFTITTEACVEYFRLKKTYPTGMWEQALAGMKKVERSMKMGFGDPERPLLVSVRS
                     GAKASMPGMMDTVLNLGLNERTLHGLIKKTGNPRFGYDAYRRFITMFGSIVLGVKREK
                     FERLLEDAKAERGVHHDTELDEHALRRLVGDFQGLVKAETGRPFPEDPMEQLTLAIKA
                     VFDSWYGARAVTYRKLNNIPESWGTAVNVVAMVFGNMGETSGTGVAFTRDPSTGERRF
                     FGECLLNAQGEDVVAGIRTPLPVESLKDTIPGAYRELLRVYKTLEKHYRDMLDLEFTI
                     QEGTLYMLQTRVGKRTAAAAIRIATEMAKEKLIDRETAILRVDPEQLDQLLHPTIDPS
                     AKLKIVAKGLPASPGAAGGKVVFTAEDAERAAGKGEKVILVRAETSPEDIGGMNAAEG
                     ILTARGGMTSHAAVVARGMGKCCVAGCSAVNIDEATDSFTVNGNVVRRGDWITLNGST
                     GEVILGEAPLIQPKWTKDFQVLMGWVDQTRRLRVRANADTPHDAKVARGFGAEGIGLC
                     RTEHMFFEGDRIRAVREMILADDLDGRQRALAKLLPMQKSDFLGIFKEMAGLPVTIRL
                     LDPPLHEFLPQTEGELASLAADMGVSVDDLRAKNKSLHEFNPMLGWRGCRLGITFPEI
                     YEMQVRAIFEAACELAQQKVKVIPEVMIPLVAHVKELARMKELLIRVAEETMKRYGKK
                     VAYTIGTMIELPRAALVADEIAKEAEFFSFGTNDLTQTTFGLSRDDAGKFLPFYVEQG
                     ILPRDPFVAIDQDGVGTLMQMGTQKGRSVR"
BASE COUNT          559 a          941 c         1015 g          455 t
ORIGIN      
        1 ggcgttccgc cggctggccg cgctcaccgc gttttcgcaa cggcccgcgt tcgagtcgtt
       61 gatgacggcc tgcaaacggg tcatgaacat cctgcccgag ccgtgccccg ccgcgatcga
      121 ccccgcgcgg ttcacgcatg acgcggaacg ccgcctgcac gcggaaacgc agcgggccgc
      181 ggccgcggtc gcggtgcacg aggagcaggg gcgggacgag gccgtgttgg agacgttcga
      241 gtcgctcaag cccgcgatcg acggattttt caccgacgtg atggtgatgg ccgatgatcc
      301 cgcggtgcgc gacacgcggc tggccctgct caaggcggtg aacgatctgt tggcgcggtt
      361 cgcggatttt cgcgcggtgg cgacgacgga aaccgcgcgg tgagaacata aggtcagaat
      421 atcttgcagc ctcggcgctg gaacccgcgc ctacgttgca ttcagcaagc tctgagggga
      481 gaacgtgatg gctcgatcag cgacgcacaa gtgggtgtac tccttcggcg acggacgcgc
      541 cgaaggcaag gccggcatga aggacttgct gggcgggaag ggcgccgggc tcgcggagat
      601 gaccaacctc aagattccgg tgccgcccgg gttcaccatc accaccgagg cgtgcgtcga
      661 gtatttccgg ctcaagaaaa cgtatcccac aggcatgtgg gagcaggcgc tcgcgggcat
      721 gaagaaggtc gagcggtcca tgaagatggg cttcggcgac ccggagcggc cgttgctggt
      781 ctcggtccgg tccggggcca aggcctccat gcccgggatg atggacacgg tcctgaatct
      841 gggcttgaac gagcgcacct tgcacgggct gatcaagaag accggcaacc cgcgcttcgg
      901 gtacgacgcg taccgccgct tcatcaccat gttcggctcc atcgtgctgg gcgtgaagcg
      961 cgagaagttc gagcgcctgc tggaagacgc caaagccgag cgcggcgtgc accacgacac
     1021 ggagctggac gaacacgcgc tgcggcggct ggtcggggac tttcagggcc tggtcaaggc
     1081 tgaaaccggc cggccgtttc cggaagatcc catggagcaa ctcacgctcg cgatcaaagc
     1141 cgtattcgat tcctggtacg gcgcgcgcgc ggtcacgtat cgcaagttga acaacatccc
     1201 ggagtcgtgg ggcactgcgg tgaacgtggt ggccatggtg ttcgggaaca tgggcgagac
     1261 cagcggcacg ggcgtggcgt tcacgcgcga cccctccacc ggcgagcgcc ggttcttcgg
     1321 ggagtgcctg ctcaacgcgc agggcgaaga cgtggtcgcg ggcatccgca cgccgctgcc
     1381 ggtcgagtcg ctcaaggaca cgattcccgg cgcgtatcgc gagttgctgc gcgtgtacaa
     1441 gacgctcgag aagcactacc gcgacatgct cgacctggag ttcacgatcc aggagggcac
     1501 gctctatatg ctccagacgc gcgtgggcaa acgcacggcc gcggccgcga ttcgcatcgc
     1561 cacggaaatg gccaaggaaa agctcatcga ccgcgagacc gcgatcctgc gcgtggaccc
     1621 ggagcagctc gaccagctcc tgcaccccac catcgacccc tcggccaaac tcaagatcgt
     1681 ggccaagggc ttgccggcgt cgccgggcgc ggcgggcggc aaggtggtgt tcacggccga
     1741 agacgcggag cgcgccgcgg gcaagggtga aaaggtcatc ctggtgcgcg cggagacctc
     1801 gcccgaggac atcggcggca tgaacgcggc cgaaggcatt ctcaccgcgc gcggcggcat
     1861 gacctcgcac gcggcggtgg tggcgcgcgg catgggcaag tgctgcgtgg ccgggtgcag
     1921 cgcggtcaac atcgacgaag cgaccgactc gtttacagtc aacgggaacg tggtgcgccg
     1981 gggcgattgg atcacgctca acggctcgac cggcgaagtc atcctgggcg aagccccgtt
     2041 gatccaaccc aaatggacca aggacttcca ggtcctcatg ggctgggtgg accagaccag
     2101 gcgcctgcgc gtgcgcgcga acgcggacac gccgcacgac gccaaggtgg cgcggggatt
     2161 cggcgcggag ggcatcgggc tgtgccggac cgagcacatg ttcttcgagg gcgaccggat
     2221 ccgcgcggtg cgcgaaatga tcctggccga tgatttggac ggccgccagc gcgcgctcgc
     2281 caagttgctc cccatgcaaa agagcgactt cctcggcatc ttcaaggaaa tggccggcct
     2341 gccggtgacc atccgcctgc tcgatccgcc gctgcacgaa ttcctgccgc aaaccgaagg
     2401 ggagctggcg tcgctggccg ctgacatggg cgtgtcggtc gatgacctgc gcgccaagaa
     2461 caagagtctg cacgaattca accccatgct gggatggcgg gggtgccgcc tgggcatcac
     2521 cttccccgag atttacgaga tgcaggtgcg cgcgatcttc gaagccgcgt gcgaactggc
     2581 gcagcagaaa gtgaaagtga ttcccgaggt catgatcccc ttggtcgcgc acgtgaagga
     2641 attggcgcgc atgaaggaat tgctgatccg cgtggccgaa gagaccatga agcgctacgg
     2701 caagaaagtc gcgtacacca tcggcaccat gatcgagctg ccgcgcgccg cattggtggc
     2761 cgacgagatc gccaaggaag ccgagttctt ctccttcggc accaacgacc tcacccaaac
     2821 caccttcggc ctctcccgcg acgacgcggg caagttcctg ccgttctacg tcgaacaagg
     2881 catcctgccc cgcgatccct tcgtggccat cgaccaggac ggcgtgggca ccctgatgca
     2941 aatgggcacc cagaaaggcc gctcggtccg
//
LOCUS       DBNGTT010000445         4959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1510373, whole genome shotgun sequence.
ACCESSION   DBNGTT010000445 DBNGTT010000000
VERSION     DBNGTT010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1510373"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..514)
                     /locus_tag="AC16BC_10085"
     CDS             complement(140..514)
                     /locus_tag="AC16BC_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242010.1"
                     /translation="MRRLNARRTIGAVVASSLVFFVIVATLCPLPSLVMAEAPALGGA
                     ASGPMECPFHPGRAASLSTALQYSLDTFKVIPGAQGPVVASDCGDAAFLSAIPRHVDY
                     AAPQGPVPTHVPLFLLHASLIR"
     gene            618..1052
                     /locus_tag="AC16BC_10090"
     CDS             618..1052
                     /locus_tag="AC16BC_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242011.1"
                     /translation="MFDRSTLRWWRPLAAVFVARTAYAGLIHGPLVLTPLVYARFPTA
                     MNPALNLPALLAVEALVSLLLVASFRAYFRSGRPDWRSAVLFGLWFGALVYVPQNLLN
                     WILLSPVRPRLAAAWIGAGLASGVVSALACRTFAPNTPRPVT"
     gene            complement(1079..1591)
                     /locus_tag="AC16BC_10095"
     CDS             complement(1079..1591)
                     /locus_tag="AC16BC_10095"
                     /inference="COORDINATES: protein motif:HMM:NF025447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HIR3242012.1"
                     /translation="MTDSRPENGRLVIRDVNTVGWHAAIVEPDQAHHGWAFSVGFTQT
                     FRHPEVAVFGLPADVLRAVLDAIGCELRSGRMFADGQDNALLAPPYRCVFRDVDAAWR
                     DLVLPGAAWFYGGRPFSALQLVWPDRGHRFPWETEFDPDLIAFQPLLFHAEASAARVS
                     AFLDRDVASG"
     gene            1704..2318
                     /locus_tag="AC16BC_10100"
     CDS             1704..2318
                     /locus_tag="AC16BC_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3242013.1"
                     /translation="MDRMAFEDQVIGLLDDLLGAAMRLAKNRQDAEDLVAESVAKAWA
                     SRRSLHDPARFRAWLFRILTNVFISECRKRGARPKADVSLDAPGDTEGAFSLFEELHQ
                     PFLLWWGNPEQEFLNKLLRRDIERAVDALPEAFRMVVVLADMEGFSYQEIGRMLGVPI
                     GTVRSRLARARSHLQKALWVHAKETPLDHRSHEACHEAGQDDRM"
     gene            2290..2529
                     /locus_tag="AC16BC_10105"
     CDS             2290..2529
                     /locus_tag="AC16BC_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3242014.1"
                     /translation="MKRVKTIGCEEALSHLLEYLDQEVGETTRHQVEHHLDACRGCFS
                     RAEFERHLRDKLRSTGRQPAGESFRERVKTLVKRF"
     gene            2556..2996
                     /locus_tag="AC16BC_10110"
     CDS             2556..2996
                     /locus_tag="AC16BC_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group I truncated hemoglobin"
                     /protein_id="HIR3242015.1"
                     /translation="MNSFRIAAVVGLSVALAAVGCARHTTPEASLYDRLGGKPAITAV
                     VGDFLQKVGSDGRIQHQPAPERVPMLTANLVDLVCQAAGGPCEYRGRPMKAAHAGMGI
                     TEGEFEAVVDDLVQTLDRYKVPEREKNELLAMLAPMRGDIVEAQ"
     gene            3074..3859
                     /locus_tag="AC16BC_10115"
     CDS             3074..3859
                     /locus_tag="AC16BC_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_391899583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3242016.1"
                     /translation="MVAITDTKKDSIFKAVSHMYTDVARCPSRAFHFPTGRPACEFVG
                     YPANELDAIPATAVESFAGVGYPFAVGAIREGDTVLDIGSGSGTDVLIASLWVGPNGR
                     VYGLDMTPAMLDKARSNAAIMNAQHVTFIEGNAEKIPLPDAAVDVVTSNGVLNLVPDK
                     PRAFAEIFRVLKPGGRIQISDIVLGKPIKETSRENPQLWAECIVGAVLEDEYVALFRK
                     AGFSGVEVLSHVDYFSRSPEPETREVAEKYGAITVILKGIKTA"
     gene            4093..4950
                     /locus_tag="AC16BC_10120"
     CDS             4093..4950
                     /locus_tag="AC16BC_10120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3242017.1"
                     /translation="MTTPTAQEHVESQRRDWNRVAPGWEKWDRVLDENLAFVNYRLAG
                     DARLRPGQRVLDLGSGTGYPALLSAQAVGSGGEVIGLDVAEDMLEVARRKAKALGLAN
                     VAFRHADVTTLPFEAGSFDAVISRFCLMFLPDVPKAVAEIARVLKPGGYVAAAVWSTP
                     DKNPFILAPINVIKTFTDVPAPAPEQPGIFRLAKPGDLLGMMARAGLTPLADDEVTGE
                     SPFASPQEYLDNLLDIAAPIQNLMAKLTDDQREAAKDGIKRAVESYRRGAGLALPMTI
                     RVVTARKPM"
BASE COUNT          923 a         1584 c         1631 g          821 t
ORIGIN      
        1 agtgccgcga gaccgatcag gaataaccga cgcaggcgta acgacatgtc atcctctcgt
       61 tgcgagtgtg aagtcgcaga acggaccaac gccccatcgc gcgaggcgat aagcgttcac
      121 cggaacaacg agcggacgat cagcggatca gcgaggcgtg gagcaaaaac agggggacgt
      181 gcgtgggaac cgggccctgc ggcgcggcgt aatcgacgtg ccgggggata gcggacaaga
      241 acgcggcgtc accgcagtca gacgcgacga ccggtccctg agcaccggga atcaccttga
      301 aggtatccag cgagtactgg agcgcggtgg acagtgacgc cgcccgcccg gggtggaacg
      361 ggcattccat tgggccggat gcggcgcctc caagggccgg ggcttcggcc atcaccagcg
      421 acgggagcgg acacagggtc gccacgatta caaaaaatac cagcgacgac gcaaccaccg
      481 ccccaatcgt gcggcgtgcg ttcagccttc gcatcgaccg actcatcgtg accacggggc
      541 caacatactc ccggcgggag agagctgtca agccgacgga ttgcacggcg tcgcggcgtc
      601 tgttacattg cgacgccgtg ttcgaccgat cgactcttcg ctggtggcgg cccctcgcgg
      661 cggtgttcgt tgcgcgaacc gcgtacgcgg ggctgatcca tggcccgctc gtgttgaccc
      721 cgctggtgta tgcgcggttt ccgaccgcga tgaacccggc gctcaacctc cccgccttgc
      781 tcgcggtcga agccctggtt tccctgctct tggtcgcctc gttccgcgcc tatttccgga
      841 gtgggcgacc cgattggcgg tccgccgtcc tgtttggact ctggttcggc gctctggtct
      901 atgtcccgca gaacctgctc aattggatcc tgctcagtcc ggtgcgcccg aggctcgcgg
      961 cggcgtggat cggagcggga ctcgccagcg gggtggtcag cgcgctggcg tgccgcacgt
     1021 tcgcgcccaa cacgccgcgt cctgtgacct gaacgagccg cctgacgcgt gagcgggatc
     1081 acccgctcgc aacgtcgcga tcgaggaagg ccgacaccct cgcggcggaa gcctctgcgt
     1141 gaaagagcag gggctggaac gcgatgaggt cgggatcgaa ctccgtctcc cacggaaacc
     1201 ggtggccccg gtcgggccag accagctgga gcgccgagaa cggacgcccg ccgtagaacc
     1261 atgcggcgcc cggcaacacg agatcccgcc acgccgcgtc gacatcgcgg aagacgcacc
     1321 gatacggcgg cgccaacagg gcattgtcct ggccgtccgc aaacattcgg ccggatcgaa
     1381 gctcgcaacc gatcgcatcc aggaccgcgc ggaggacgtc ggccggcagc ccgaagaccg
     1441 cgacctccgg atggcgaaac gtctgcgtga atcccacgga gaacgcccag ccgtggtgcg
     1501 cctggtccgg ctccacgatc gcggcgtgcc acccaacggt gttaacgtcg cggataacga
     1561 gacggccgtt ttcgggacgg gaatccgtca tagagaccac gttagcacaa aagccgtaac
     1621 gaagctcgcc gccctcaacg gtcgggaact ttttgtgacg atccgcgtct aagcgggtgt
     1681 agaaagtaca tggggtggac gcaatggacc ggatggcgtt cgaagatcag gtaattgggc
     1741 tgctcgacga cctgctgggg gcggccatgc ggctcgccaa aaaccggcag gacgccgaag
     1801 atctggtggc cgagtcggtg gccaaggcct gggccagcag acgttcgctg cacgatcccg
     1861 cccgcttccg ggcgtggctg tttcggatcc tgacgaacgt gttcatcagc gagtgtcgga
     1921 agcgcggcgc ccgcccgaaa gccgacgtgt ccctggacgc gccgggcgac accgaggggg
     1981 cgttttcctt attcgaagag ctgcaccagc cctttttgtt atggtggggc aaccccgagc
     2041 aggagtttct caacaagctc ctccgccgtg acatcgaacg cgcggtggat gccctgccgg
     2101 aggcctttcg catggtggtg gtcttggcgg acatggaagg gttttcgtat caggagatcg
     2161 gtcggatgct gggcgtcccg atcggcacgg tgcgatcgcg cctggcgcgg gcgcgcagcc
     2221 acttgcagaa ggcgctctgg gtccatgcga aggagacgcc gttggatcat cgctcacacg
     2281 aggcatgcca tgaagcgggt caagacgatc ggatgtgaag aagccttgtc ccacctcctc
     2341 gagtacctcg atcaggaggt gggagagacc acgcgccatc aggtcgaaca tcatctggac
     2401 gcgtgcagag gttgtttttc gcgcgcggag ttcgaacggc atttacggga caagctccgc
     2461 tcgacaggcc ggcagccggc aggcgagtcg tttcgagagc gggtcaaaac cctggtgaag
     2521 cggttttagc caccaatcac tgaggaggac actcgatgaa ctcatttcgc atcgccgccg
     2581 tggttggact ttccgtcgcg ctcgcggccg tcggctgcgc gcgccacacc acacccgagg
     2641 cgtcgctcta tgatcgcctg ggcggcaaac ccgctattac cgcggtcgtc ggggatttcc
     2701 tgcaaaaggt gggatcggac ggtcggatcc agcaccagcc cgctcccgaa cgcgtcccga
     2761 tgctgaccgc caacctggtg gatcttgtct gccaagccgc cggcggaccg tgcgagtacc
     2821 gcggcaggcc gatgaaggcc gcgcacgccg ggatggggat caccgaaggc gagttcgagg
     2881 cggtggtcga cgatctcgtc cagaccctcg accgctacaa agtgcccgaa cgggagaaaa
     2941 acgagctgct cgccatgttg gcgccgatgc ggggcgacat cgtggaagcg caataacgat
     3001 gcccccgtag catcggcgcc cttccgtgga gggcggcaca acgcccattg cgacaccgac
     3061 aaaggagata cccatggtcg cgatcaccga taccaaaaag gattcgatct tcaaggccgt
     3121 ctcgcacatg tacaccgacg tggcccgttg ccccagccgg gcgttccact tccccacggg
     3181 ccgccccgcg tgcgagttcg tggggtaccc agccaatgaa ctcgacgcga taccggcgac
     3241 cgccgtggag tcgttcgcag gcgtggggta cccctttgcc gtgggagcga tccgggaggg
     3301 cgacaccgtg ctcgacatcg gctcgggctc gggaaccgac gtgctcatcg cctcgctgtg
     3361 ggtcggcccg aacggcaggg tatacggcct ggacatgacg ccggccatgc ttgacaaggc
     3421 gcggtccaac gccgccataa tgaacgccca acacgtgacg ttcatcgaag ggaacgccga
     3481 gaagatcccg cttcccgacg ccgcggtgga tgtcgtgacc agcaacggcg tgttgaacct
     3541 cgtgcccgac aaaccccgtg ccttcgccga gatcttccgc gtcctcaagc ccggtggccg
     3601 gatccagatt tcggacatcg tgctcggcaa acccatcaaa gagacgtcgc gggagaatcc
     3661 ccaactctgg gccgagtgca tcgtgggggc ggttctcgag gacgagtatg tggcgctctt
     3721 ccgcaaggct ggtttttccg gcgttgaggt gctgtcccac gtcgattact tttcgcgaag
     3781 tcctgagccg gagacccgcg aggtcgcgga gaaatacggc gccatcacgg tcattttgaa
     3841 gggaatcaag acggcatagg ttcggcgctg aattgaccga tcgcttggcg ccacggtcag
     3901 cagccttgtt gtggtgtcgg gaggcgagac caccgtccag catccaggct ggtttcgccg
     3961 aataccctgc gctgacgaag cgacggtcgc acgacactgg caacgaggat tgaccggatg
     4021 gtcgtgcggt tgtgagcgtt ggcaccaagt gctgacccgt cgtacaatcg ctccatcaca
     4081 ccggagagtc ttatgacgac gcccaccgcc caagagcacg tcgagagcca acgccgggac
     4141 tggaaccgcg tcgcgcccgg ttgggaaaag tgggatcgag tgctcgatga gaacctcgcc
     4201 ttcgtcaatt atcgattggc gggagacgcg cggctccgcc cgggccagcg cgtgctggat
     4261 ctgggctccg gaaccgggta ccccgcgctg ctctccgcgc aggccgtggg cagcgggggc
     4321 gaggtcatcg gcctggacgt ggccgaagac atgctcgagg tggctcgccg caaggccaag
     4381 gcccttggcc ttgcgaacgt ggcatttcgc cacgcggatg tgaccaccct gccgttcgaa
     4441 gcgggttcct tcgacgccgt gatctcccgg ttctgtctga tgtttcttcc ggacgtgccc
     4501 aaggccgtgg ctgaaatcgc ccgcgtgctc aaacccggcg gctatgtcgc ggccgcggtc
     4561 tggtccacgc ccgataagaa tcccttcatc ctggcgccga tcaacgtcat caaaaccttc
     4621 accgacgtgc ccgctccggc gccggagcaa cccggcatct tccgtctcgc caaaccggga
     4681 gacctcctcg gcatgatggc gcgcgcgggc ctgactcctc tggccgacga cgaggtcacc
     4741 ggggagtctc cgttcgcctc cccccaggaa tacctggaca acttgctgga catcgcggcg
     4801 ccgattcaga atttgatggc caaactcacg gacgaccagc gggaggccgc gaaggacggg
     4861 atcaaacgcg cggttgaatc ctaccggcga ggcgcgggat tggccttgcc catgacgatc
     4921 cgcgtggtca ccgcgcgcaa accaatgtag ggagaagca
//
LOCUS       DBNGTT010000446         1792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2023238, whole genome shotgun sequence.
ACCESSION   DBNGTT010000446 DBNGTT010000000
VERSION     DBNGTT010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2023238"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="AC16BC_10125"
     CDS             complement(<1..643)
                     /locus_tag="AC16BC_10125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3242018.1"
                     /translation="MTRRDANVRGPLTAALIKRYKALADKKGRTASGLFLVEGPRAVR
                     QVLTSRPEAVVEIIATAEHASHYEGVPVRTATHAQLDGICHTKTPQGAVAVVHQPDHV
                     YEDQLPDSVGPRVLLLEEIQDPGNVGALIRTAAAFDYSLVILSDGCADPLAPKCVQAS
                     SGTILSVEIRRTSAYLNLAHALRDHGHVMAVADVRGEDDPSRLKEPEHLVFALG"
     gene            complement(646..>1792)
                     /locus_tag="AC16BC_10130"
     CDS             complement(646..>1792)
                     /locus_tag="AC16BC_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3242019.1"
                     /translation="PVQSVMVAEVYGPDEPVRRQAAASVVRAFNEAPGVVGIDDTLEA
                     ARPKLVVTVDQAKAAASGVATSDIVYALRLGVTGVPAGRMIDPREKEPVAALLRLPYE
                     KRSAIDSLLNVRLRATGGGMVPISELVRVERPLEEPPRYHKNLRPVVYVTGDVAGREE
                     SPVYGMLAINERLDALRAPDGSPVTLLYAAQPRSEERVAVKWDGEWQITYETFRDMGI
                     AFGIALVLIYLLIVGQFQSFLIPLVIMAPIPLTLVGVIPGHWITGSYFTATSMIGFIA
                     LAGIVVRNSILLVDFIQAEQAAGRSLTDAVLRAGAIRTRPILLTAAALMVGAFVIILD
                     PIFQGLAISLLFGVLGSTLLTLVVIPVLYVTLAELTPTGAARSNHEE"
BASE COUNT          296 a          631 c          594 g          271 t
ORIGIN      
        1 tgcccagggc gaagaccagg tgctccggct ccttcagccg cgacgggtcg tcttccccgc
       61 gcacgtccgc gaccgccatc acatggccgt gatcacggag cgcgtgggcc agattcaagt
      121 aggccgacgt gcgccggatc tccacggaca ggatggtccc gctggaggcc tgcacgcact
      181 tgggcgcgag cgggtccgcg cagccgtcgc tgaggatcac gagcgagtag tcgaacgcgg
      241 ccgcggtgcg gatcagcgcg cccacgttgc cggggtcctg gatctcttcg agcagcagca
      301 cgcgcggtcc cacggagtcc ggcaattgat cctcgtacac gtggtccggt tggtggacga
      361 cggccaccgc gccctgcggg gtcttggtgt ggcagatgcc gtcgagttgc gcgtgcgtgg
      421 ccgtccgcac cggaacgccc tcatagtgcg aggcgtgttc cgcggtcgcg atgatctcca
      481 ccaccgcctc gggccggctc gtcagcacct gccggaccgc gcgagggcct tccaccagaa
      541 acagcccgga cgccgtgcgc ccctttttgt cggccagggc cttgtagcgt ttgatcagcg
      601 cggcggtcaa cggtccgcga acattggcgt cacgacgggt cacggtcact cctcatggtt
      661 tgaccgggcc gcgcccgtcg gggtcagctc cgccagcgtc acgtacagga ccggaatcac
      721 gaccaacgtc aacagagtcg atccgagcac cccgaacaag agcgagatgg cgaggccctg
      781 gaagatgggg tccaggatga tcacgaacgc gcccaccatg agcgcggccg cggtcagcag
      841 gatcggacgc gtgcggatcg cgcccgcgcg cagcaccgcg tcggtcaatg accgccccgc
      901 ggcttgttcg gcctggatga aatccaccag caggatggaa ttgcggacca cgatgcccgc
      961 gagcgcgata aaaccgatca tggacgtggc ggtgaagtag gagcccgtga tccagtgccc
     1021 ggggatcacg ccgaccagcg tgagcggaat cggcgccatg atgaccagcg ggatcaggaa
     1081 cgactggaac tggcccacga tcaagaggta gatcagcacc agcgcgatgc cgaacgcgat
     1141 ccccatgtcg cggaacgttt catacgtgat ctgccactcc ccgtcccact tcaccgcgac
     1201 gcgctcctcc gatcgcggct gcgcggcgta caggagtgtg accggcgacc cgtccggcgc
     1261 ccgcagcgcg tccaggcgtt cgttgatagc cagcatgccg tagaccgggc tctcttcgcg
     1321 acccgccacg tcgcccgtga cgtagaccac cgggcgaagg ttcttgtgat agcgcggcgg
     1381 ctcttccaac ggtcgctcca cccggaccag ttcagagatc ggcaccatgc ccccgcccgt
     1441 tgcgcgcagc cgcacgttca gcaagctgtc gatcgcgctg cgcttctcgt acggcagccg
     1501 cagcaacgcc gcgaccggct ctttttcgcg cggatcgatc atgcggccgg ccggcacgcc
     1561 ggtcacgccc aacctgagcg cgtacacgat gtcggaggtg gccacgccgc tcgccgcggc
     1621 tttggcctgg tccaccgtga ccacgagctt gggccgcgcg gcttcgagcg tgtcgtcgat
     1681 gcccaccacg cccggcgcct cgttgaacgc gcgcaccacg gaggccgcgg cctggcggcg
     1741 cacgggctcg tcgggaccgt agacctcggc caccatcacg gactgcaccg gc
//
LOCUS       DBNGTT010000447         1612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3300297, whole genome shotgun sequence.
ACCESSION   DBNGTT010000447 DBNGTT010000000
VERSION     DBNGTT010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3300297"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="AC16BC_10135"
     CDS             <1..581
                     /locus_tag="AC16BC_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3242020.1"
                     /translation="AMGVFAAEGRRVEPAGLRSVTDHAGNVLAYYEPAPVDVVSRETA
                     YLITNMLEDVVQSGTGMRARALRRPVAGKTGTTNEFADAWFVGFAPNLAAGVWVGFDD
                     RRSLGDREAGASVALPIWVEFMKDALTQLPVQAFAIPDGVVFAKVDPKTGDLAAPGAP
                     AKVEMFVRDTEPTVVSRPTASIARFRQIDQGM"
     gene            742..817
                     /locus_tag="AC16BC_10140"
     tRNA            742..817
                     /locus_tag="AC16BC_10140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:Arg,seq:ccg)
     gene            832..907
                     /locus_tag="AC16BC_10145"
     tRNA            832..907
                     /locus_tag="AC16BC_10145"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:865..867,aa:Lys,seq:ctt)
     gene            1033..1117
                     /locus_tag="AC16BC_10150"
     tRNA            1033..1117
                     /locus_tag="AC16BC_10150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1067..1069,aa:Leu,seq:tag)
     gene            1191..>1612
                     /locus_tag="AC16BC_10155"
     CDS             1191..>1612
                     /locus_tag="AC16BC_10155"
                     /inference="COORDINATES: protein motif:HMM:NF017508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HIR3242021.1"
                     /translation="MKIAIETLSPVKRALSIEIEPSVVGQEFAEAYSELGRRVKVPGF
                     RPGKVPLPVLEKRFEREVTDDVVKRLVPRYYEQAIKDAGLVPVQLPTIEELSISRDAP
                     LSFRAVVEVRPSVALQPYKGIPLTRRRVQVTDAVLAEA"
BASE COUNT          259 a          461 c          560 g          332 t
ORIGIN      
        1 cggccatggg cgtgttcgcg gcggaggggc gacgggtcga gccggccggt cttcggtccg
       61 tgacggatca cgccgggaac gtgcttgcct attacgagcc cgcgccggtc gacgtggtct
      121 cgcgggagac cgcgtatctg attacgaaca tgttggagga cgtggtgcag tccggaaccg
      181 gaatgcgagc ccgcgcgttg cggcgtccgg tggccggaaa gaccgggacc acgaacgaat
      241 tcgcggacgc ctggttcgtg gggtttgccc cgaacctggc cgctggcgtg tgggtgggat
      301 ttgatgatcg tcgttcgctc ggggatcgcg aagcgggcgc gtcggtggcg ttgccgatct
      361 gggtggagtt tatgaaggac gccttgaccc aactgccggt ccaggcattt gcgattccgg
      421 acggagtcgt cttcgccaaa gtggatccca agaccggtga cctggcggct ccgggtgcgc
      481 cggcgaaagt ggagatgttt gttcgcgaca ccgagccgac ggtggtctcg cggccgaccg
      541 cgagcatcgc gcgattccga cagatcgacc aagggatgtg atgcgtcggt tgcggcccac
      601 gcgcatgatc tcgatcggcg tcggttttga caaaccgttt ggggctccgg tagaatacgc
      661 gcacttttcg ggggcgcttg gcgcagggtc ttctcgttga atcagaagtt ccgagcgccc
      721 gtccacgcgg ggcggagact tgcgcctgta gctcagcgga tagagccatg gcctccggag
      781 ccatgtgtcg ggggttcaat tccctccagg cgcgccagtg tcgatatacg tgggccgtta
      841 gctcagctgg tagagcagct gactcttaat cagcgggtcg taggttcgat ccctacacgg
      901 ctcaccactc ttttcgccgg cgccgccgga cgccgtagat ccacgaatca agccgattgg
      961 tttcggacgg ggcgcgccgg ggcaacggtg agcgcgccat cggcgggcgg tgtattgttg
     1021 gctgcgggtc gtgcgagagt ggcggaattg gcagacgcgc cagacttagg atctggtggg
     1081 tcaccccgtg ggggttcaag tcccccctct cgcaccagca aggcaggtgc gttgtgggtc
     1141 gtatcgatgt tttgtctgtc tatcactatc accctttgtc ctgagcccct atgaaaatcg
     1201 ctatcgagac gttatcgccg gtcaaacgcg cgttgtccat tgagatcgag ccgtctgtcg
     1261 tcggccaaga attcgcggag gcgtattcgg aactcggccg tcgcgtcaaa gttccgggat
     1321 tccgccccgg gaaggttccg cttccggtgc tggagaagcg gtttgaacgc gaggtgaccg
     1381 atgatgtcgt caagcggttg gtcccccggt actatgaaca ggcgatcaag gacgccggat
     1441 tggtgccggt gcaactgccg accatcgagg aattgtcgat ctccagggac gcgcctctgt
     1501 ccttccgcgc ggtggtcgag gtccgcccgt ccgtggcctt gcaaccgtat aagggcatcc
     1561 cgttgactcg acgccgcgtc caggtcacgg acgccgtttt ggcggaagcc tt
//
LOCUS       DBNGTT010000448         5301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1894905, whole genome shotgun sequence.
ACCESSION   DBNGTT010000448 DBNGTT010000000
VERSION     DBNGTT010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1894905"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1654)
                     /gene="zwf"
                     /locus_tag="AC16BC_10160"
     CDS             complement(110..1654)
                     /gene="zwf"
                     /locus_tag="AC16BC_10160"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR3242022.1"
                     /translation="MSETAVMNEAAADTKRGPTAQPCTLVIFGGSGDLARRKLIPALY
                     NLLLDGVLPQEFAVLGVGRTVLDDAGFRAMAREGIQAHSRQALDPVKWDAFERRLFYS
                     SGEVDDPATYQTIRSRAETIESQLGLPGNRIFYLAIPPTAFAAAAQGLRGAGLTQAPQ
                     PNGPFSRVIVEKPIGRDLASARQINEVLSRAFDESQIFRIDHYLGKETVQNLLVLRFA
                     NGLFEPLWNHKYIDHVQISVAEEEGIGSRASYYEEAGALRDMVQNHILQLLCLVAMEP
                     PWSLDPDVVRNARLEVLRSLRPITGHGVNDVTVRGQYSPGMVGAVEVPGYRREPDVKP
                     DSTTETYVGLKVFVDNWRWAGVPFYLRTGKRMTKRATEIAVQFKEVPPVLFNASPATP
                     LEPNILALRIQPEEGFSLHINSKLPGSRVRIEPVAMDFRYGAAFGGASPEAYERLLLD
                     VMAGDATLFMRRDAVEAAWEWVTAILDGWREHGTKWLPEYQAGTCGPVEADRLIERDG
                     RRWRAL"
     gene            complement(1670..2572)
                     /gene="gnd"
                     /locus_tag="AC16BC_10165"
     CDS             complement(1670..2572)
                     /gene="gnd"
                     /locus_tag="AC16BC_10165"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR3242023.1"
                     /translation="MEIGFIGLGKMGLNMVTRLTQGGHRVVAFDRDSAKVADAVRSGC
                     TGASSLDELISKLPAPRAVWIMVPAGAPTEDTVRDVAARLSPGDVVIDGGNTHFHDDV
                     RRAETLKGRGIHYVDAGTSGGVWGLKLGYCLMIGGTDDVVRRLAPIMTTLAPENGWAH
                     VGGVGAGHYVKMVHNGIEYGMMQAYAEGFELLSKSEYRLDLARIADLWMHGSVVRSWL
                     LELTAGALAGDPKLERLTGHVADSGEGRWMIADALEKNVPVPTLTAAIFTRFRSRQAE
                     SFAEKLLAALRNAFGGHAVKPSGG"
     gene            complement(2633..5293)
                     /locus_tag="AC16BC_10170"
     CDS             complement(2633..5293)
                     /locus_tag="AC16BC_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3242024.1"
                     /translation="MEETRARHAHWRRWGPYLSERQWGSVREDYSATGTAWEYFPHDH
                     ARSRAYRWGEDGIAGISDNHQRLCFALALWNGRDSILKERLFGLSGNEGNHGEDVKEC
                     YWYLDNTPTHSYMRFLYKYPQQAFPYDELIAENRCRGREAPEYELVDTGAFDDDRYWD
                     VFVRYAKGSPDDILIEIRVVNRGPDPAVLHVLPTLWYRNRWAWDRRVEKPLVKAGRNG
                     RGYRVIEASESTLGKRRLFCEGTPDLLFTENETNSERLFNAPNGCRYVKDGINDYVVY
                     GRADAVNPDRIGTKAAAHYCLDTSAGGETIIRLRLRSDAMAGPFADFGELVAEREREA
                     DEFYRRVTPSAPSESLLCIQRQAFAGMLWNRQHYHYVVEDWLAGDPAGPQPPRERRTG
                     RNRDWTHLYTDDILSMPDKWEYPWFAAWDLAFHVVPFAMIDPEFAKHQLVLLTREWYM
                     HPNGQIPAYEWAFGDVNPPVHAWAAWRVYKIEKKLYGRADTFFLERVFQKLLLNFTWW
                     VNRKDAEGKNIFQGGFLGLDNVSVFDRSTRLPTGGHIEQSDGTSWMAFYCLNLLAIAL
                     ELAKTNPSYEDIASKFFEHFLYIADAMNHLGGTGLWDEEDGFYHDVLHLPDGTAMPLK
                     LRSMVGLIPLFAIQTLEPDVMERLPGFARRLEWFIANRPDLSRNVASMDRPGVGKRRL
                     LSIVSASKLRRILQRMLDEQEFLSPYGVRSLSRVYGRDPYVLLVDGHESVVRYEPAES
                     SGGLFGGNSNWRGPVWFPVNYLIIESLQKFHHYLGDGFTVECPTGSGRWLTLSEVAAE
                     LSRRLIAIFLPDAEGRRPVHGHAAERYADPHWRDHVPFYEYFHGEHGAGIGASHQTGW
                     TGLVAKLIQQAAGASANARPTKGSSRRIAA"
BASE COUNT          948 a         1839 c         1568 g          946 t
ORIGIN      
        1 ggcttctcgt tcgatgtccc gacccgaggc cgtcggacgc acgagggcca gcaccgtttt
       61 cgtcccgccg atgtccccgg ccaagatcat gggtgtgtgc agcggacggt cagagcgcgc
      121 gccaacggcg accgtcgcgt tcgatcagcc gatcggcctc gaccggtccg catgtcccgg
      181 cttgatactc gggcagccac ttcgtcccat gctcgcgcca gccgtccaag atcgccgtca
      241 cccattccca cgcggcttcc accgcgtcgc gtcgcatgaa cagcgtggcg tcgccggcca
      301 tgacatcgag caacaggcgc tcataggctt ccggcgaggc cccgccgaac gccgccccgt
      361 accgaaaatc catggccacg ggttcgatgc gaacccgcga gccgggaagt ttggaattga
      421 tatgaagcga aaagccctct tcgggctgaa tgcgaagcgc caggatattg ggctccaggg
      481 gggtggcggg gctcgcgttg aacagaaccg gcggcacctc tttgaactgc accgcgatct
      541 ccgtcgcccg cttggtcatc cgtttgccgg tccgcaggta aaatgggacg ccggcccatc
      601 gccaattatc cacgaacacc ttcagcccca cgtatgtctc ggtcgtggag tccggtttca
      661 cgtcgggttc gcgccgataa cccggcacct ccacggctcc caccatcccg ggactgtatt
      721 ggccgcgcac cgtgacgtcg ttgaccccat gccccgtgat cggccgcaac gatcggagca
      781 cctcaaggcg ggcgttgcgg accacgtccg ggtccagcga ccacgggggc tccatcgcga
      841 ccaggcacaa caactggaga atatgattct gcaccatgtc gcgcagcgcg ccggcttcct
      901 cgtagtacga ggcgcgcgac ccgatcccct cttcttccgc caccgagatt tgcacgtggt
      961 cgatgtattt atggttccac agcggctcga agaggccgtt ggcgaatcgc agcaccagga
     1021 gattctggac cgtctctttg cccaaataat ggtcgatccg gaaaatctgc gactcgtcga
     1081 acgcgcggct caagacttcg ttgatctgcc gggccgacgc cagatcgcga ccgatgggct
     1141 tctcgacgat cacgcgcgag aacgggccgt tcggctgggg cgcctgcgtg agcccggctc
     1201 cgcggaggcc ctgtgccgcg gccgcgaacg ccgtcggcgg gatcgccaaa tagaagatgc
     1261 ggttcccggg aagtccgagt tgcgattcga tggtttccgc gcgcgaacga atcgtctgat
     1321 acgtggcggg atcgtccacc tcgccggatg aatagaacaa gcgccgctcg aaggcatccc
     1381 acttgaccgg atcgagcgcc tggcgcgagt gcgcctgaat accctcacga gccatggcgc
     1441 gaaatcccgc atcgtccagc acggtgcgcc ccacgcccag caccgcgaac tcctgcggga
     1501 gaacgccgtc gagtaacagg ttatagaggg ccgggatcag cttgcgccgc gcaaggtctc
     1561 ccgatccccc gaagatcacc agcgtgcagg gttgcgcggt cggtccccgt ttggtgtcag
     1621 ccgccgcttc gttcatgacc gccgtctccg acatggctcc ctccgtctcc tacccgcccg
     1681 agggcttgac cgcgtgcccg ccgaacgcat tgcgcagggc cgccagcagc ttctctgcga
     1741 acgactccgc ctgacgcgaa cgaaaccggg taaagatcgc cgcggtcaac gtgggcaccg
     1801 gcacgttctt ctccagcgcg tccgcgatca tccaacgtcc ttcgcccgaa tccgccacgt
     1861 gccctgtgag ccgctccagt ttcggatcgc cggccagtgc gcccgccgtc agttccaaga
     1921 gccacgaccg caccacgctg ccatgcatcc acaaatcggc gatgcgggcg agatccaaac
     1981 gatactcgct tttggaaagc aactcaaacc cctcggcgta cgcctgcatc atgccgtatt
     2041 cgatgccgtt gtgcaccatt ttcacgtagt ggccggcgcc gaccccgccc acgtgcgccc
     2101 agccgttctc cggcgcgagc gtggtcatga tcggggcgag ccgccgaacc acgtcatccg
     2161 ttccgccgat catgaggcag tatcccagct tcagccccca cacgccgccg ctggtgccgg
     2221 catccacgta gtggatcccc cggcccttga gcgtctcggc gcgccggaca tcgtcgtgaa
     2281 agtgcgtatt ccctccgtcg atgaccacgt cgcccgggct caaccgcgcc gccacgtcgc
     2341 gcacggtgtc ctcggtcggc gcgccggccg gaaccatgat ccagaccgcc cgtggcgcgg
     2401 ggagtttgga aatcagctcg tcgagcgacg acgcgcccgt gcacccgcta cgcaccgcgt
     2461 cggccacctt cgcggaatcg cgatcgaacg ccaccacccg gtgcccgccc tgcgtcagcc
     2521 gggtgaccat attcagcccc attttcccaa gaccaataaa tccgatctcc atctcgcacc
     2581 ctttccgccg tctccctccg atggagacgg ctggttccgg tctgctcgcc gcttaagccg
     2641 cgatccgtcg tgacgacccc ttggtgggcc gcgcgttcgc cgacgccccc gcggcctgct
     2701 ggatcaactt cgcgaccagg ccggtccacc cggtttgatg gctcgcccca atccccgcgc
     2761 cgtgttcacc gtgaaagtac tcgtagaacg ggacgtggtc ccgccagtga ggatcggcat
     2821 accgctccgc agcgtgcccg tgtacgggcc gccgcccttc ggcgtccggc agaaagatcg
     2881 cgatcaatcg gcgcgacagt tccgccgcca cctcggacaa cgtcaaccag cgtcctgatc
     2941 cggtcgggca ttccacggtg aacccgtcgc ccagatagtg atgaaacttc tggagcgatt
     3001 cgatgatcag gtaattgacg gggaaccaca ccggcccccg ccagttggag ttcccgccga
     3061 acagcccgcc cgacgactcc gccggctcgt agcggaccac gctctcgtgg ccgtcgacca
     3121 gcaggacgta gggatcgcgt ccatacacgc gggacaggga tcgaacgccg taggggctca
     3181 ggaactcctg ttcatccagc atgcgctgga gaatgcgccg cagcttgctc gcgctcacga
     3241 tggacagcag ccgccgcttc cccaccccgg gccggtccat gctcgccacg tttcgggaca
     3301 gatcgggccg gttcgcgatg aaccactcca gccggcgcgc gaaccccggc agccgctcca
     3361 tgacgtcggg ttccaaggtc tggatggcga acaacgggat caatccaacc atcgagcgca
     3421 gcttgagcgg catcgcggtg ccgtccggca aatggagcac gtcatggtag aacccatcct
     3481 cttcatccca cagtcccgtg ccgcccagat gattcatcgc gtccgcaata tacaagaagt
     3541 gctcaaaaaa cttgctcgcg atgtcttcgt agctcgggtt ggtcttggcc aactcgagcg
     3601 caatggccaa gaggttgagg cagtagaacg ccatccaact cgtcccgtcg gattgctcga
     3661 tatggcctcc ggtcggcaac cgggtgctgc ggtcgaacac cgagacatta tcgagtccca
     3721 gaaatccgcc ctggaaaatg ttcttgccct ccgcgtcctt tcggttgacc caccacgtga
     3781 agttgaggag cagtttctgg aatacgcgtt cgaggaaaaa cgtgtcggcg cgcccgtaca
     3841 gtttcttctc gatcttatac acccgccatg ccgcccacgc atgaaccggc ggattcacgt
     3901 ccccgaacgc ccactcgtac gcggggatct gcccgttggg gtgcatgtac cattcgcggg
     3961 tcagcaacac gagttgatgt ttggcgaact ccggatcgat catcgcgaac ggcaccacgt
     4021 ggaacgcgag gtcccacgcc gcgaaccagg ggtactccca cttgtcgggc atcgacagga
     4081 tgtcgtccgt gtacaggtgg gtccagtccc gattgcggcc ggttcgacgc tcgcgaggcg
     4141 gctgaggtcc cgcggggtcg ccggccaacc agtcctcgac cacgtaatgg tagtgctgac
     4201 ggttccacaa catccccgca aaggcctgcc gctggatgca tagcaggctc tctgatgggg
     4261 ccgacggcgt cacgcgccga taaaactcat cggcctcgcg ctcccgttcg gcaaccaact
     4321 cgccgaaatc cgcgaacgga ccagccatcg cgtccgaccg aaggcgaagg cggatgatcg
     4381 tttcgccgcc tgccgacgtg tccagacaat agtgcgccgc ggccttggtg ccgatgcggt
     4441 cgggattgac cgcgtcggct cgaccgtaca ccacgtagtc gttgatcccg tctttcacgt
     4501 agcggcaccc gtttggagcg ttgaacaggc gttcggagtt agtctcattc tcggtgaaga
     4561 gcaggtcggg cgtgccttca cagaagagcc gtcgtttgcc caacgtggac tcggacgcct
     4621 cgatcacgcg gtatccccgc ccgtttcgtc ccgccttcac caacggtttt tccacgcgcc
     4681 ggtcccacgc ccaccggttc cggtaccaga gcgtggggag cacgtggagc acggccgggt
     4741 cgggaccgcg gttgaccacg cggatctcga tcaatatatc gtccggagat cccttggcat
     4801 accggacgaa cacgtcccaa taccggtcgt cgtcgaatgc cccggtgtct accagctcgt
     4861 actcgggcgc ctcgcggccg cgacaacggt tttccgcgat caactcgtca tagggaaacg
     4921 cctgttgcgg gtacttatag agaaatcgca tgtacgagtg cgtgggcgtg ttgtcgaggt
     4981 accaataaca ctccttcacg tcctcgccgt gattgccttc attcccagac agcccgaaca
     5041 accgctcttt cagaatggaa tcgcggccgt tccacagcgc cagcgcgaaa cagaggcgtt
     5101 gatgattgtc ggaaatgccg gcgatcccgt cttctcccca acgataggcc cgcgaacgcg
     5161 cgtggtcgtg ggggaaatac tcccacgcgg tgccggtcgc gctatagtcc tcgcgcacgc
     5221 tgccccactg ccgttcgctc aggtaggggc cccagcggcg ccaatgcgcg tggcgcgcac
     5281 gcgtctcttc cagtcggcgt t
//
LOCUS       DBNGTT010000449         5249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2278498, whole genome shotgun sequence.
ACCESSION   DBNGTT010000449 DBNGTT010000000
VERSION     DBNGTT010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2278498"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="AC16BC_10175"
     CDS             <1..546
                     /locus_tag="AC16BC_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242025.1"
                     /translation="VDPSIAKIPPGSGPNQIPVAGYTRVSADGKTVFTVGYDATAGKS
                     LLSAIDASTSDAVLGVLDLPDIGVASFDEKAGDRLYLPSNDLGAKKNVIAVVDVNPSS
                     PTYLSVIGEITVGEALGDRHGEVSTDGRVAVYPDTCAACNAVNVIETATDSVVTTLTL
                     LGPPAKAVGMVRWPVTATENH"
     gene            640..3354
                     /locus_tag="AC16BC_10180"
     CDS             640..3354
                     /locus_tag="AC16BC_10180"
                     /inference="COORDINATES: protein motif:HMM:NF040138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR3242026.1"
                     /translation="MFLATRAVSQSTSLGRCFRFAAWLILLGVIAGCGGGTGGDSGSQ
                     GLIGPAGGTVTSADGKVSLVIPPNAVGVTTQFAIDPDPANGGAPSPRIVGTVYSIDSD
                     PTGISFPANALPQLTMPYPSGVADPSTLTIGTLENGQWVNVAGSQVNQANHTVTAALA
                     HLSPYAVLQLASGTDNNPPIASVGGPYQGEVGQLIQFDAVGSIDPDRDPLTYTWRFGD
                     GSPAVSGPQQSADHRYATAATFTVTLTVSDGRGGSDSKSTTATIVSAPPVNHQPTANA
                     GGPYAATVGQLITFDASASTDPDQDPLTYDWNFGDGATAANAGPAPTHTYNTTSGSPF
                     TVTVTVRDPSESFSSATATATISPLPPVNHAPAITSMMMPTPSTVIAGRSILVDGTAT
                     DADGDLLTYSWNFGDGSSSTPAISRSDATHTYNAAGPYGPYEVKLSVSDGQGGDVFDT
                     RNLVVEAPPVNHAPVAHAGGPYVGVVGQPLAFDASASSDQDQDPLTYSWTFGDGATGD
                     GVAVTHAYSTSAGSPFTVTVTVTDPGGLSGSASTTATITLPPNQPPIAVVNGPYAGTV
                     GQVITFSAAGSADPDNDPATLTYNWEFGDGATGSGLSTTHAYTTAATYPVKVTVTDPA
                     GASSSASTTAAITAPTPGNRLPVISVNVPPQALIFQQVVLSGSGSDPDGNTLSYSWNF
                     GDGTTSAFVATASAAHQYQTAGTYTPTLTADDGHGGVVSVSGSVTVVVPAQPVAFPAQ
                     LKVYIRQNVQAPHRWHYAVPLHGSGVPPLTFSIVAFPTHQDASLPTMAMAATQYYQWL
                     DPVTSEWRVECQSSTPAAACMATTSSSVNLATGTVVDPNTSSPIQVPSTLSPIVVYAP
                     ANCNDATWGIGQYSDAFAFTVTDGTGVTSAPATVSISFFDTGCAGGTH"
     gene            3530..4960
                     /locus_tag="AC16BC_10185"
     CDS             3530..4960
                     /locus_tag="AC16BC_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242027.1"
                     /translation="MSAALLANSVFLLMRTDAEAIPAFARKYDMDCSHCHSMAPKLNR
                     VGLKFHDNFTLKEALGDLDPELRDRVRNEDPEDMHPAYWPVSFRIAGGYQYNKRDNQP
                     VDSAGLDLRTISTNTFALERFELLAGGLFAPGISYYISYYPEALNVGLPGQPPVHAHP
                     GATSGSSQLGALGFAWARFADIFGQTAQDEHHDAAADGHDHEAAEPKHDHGQDLILGS
                     HELHLTLSGHHRLTNAPYLAYRYDPLYVLDPAKGFKLDAPQLGASLDGSTPWFGYAVS
                     LYNGTSNSADDNRTLDVFATINQDFGDNRVGVFGLRGTSPTSWIMTTTTSPPSPVPGT
                     GSANKPYFRFGADTDLNVGPLNVLFFALYGQVDKDLFDPSLGQTAKFYAGFLETDYLI
                     ESVRTMLIARYDGVRNVSQGLSTYAHNRGDTDAFTLAVRRDLVLTSRANLQVHVEANA
                     TRVQAIATNDNDQTSNTFFAGVDWSF"
     gene            5014..>5249
                     /locus_tag="AC16BC_10190"
     CDS             5014..>5249
                     /locus_tag="AC16BC_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242028.1"
                     /translation="MSTSAFGWTRAAAAAFALAAAGCASGSAPVDGQSGGSTSDVFTI
                     SGSLTTPVGVTLGPGASGSITFSVKGSDPASTFY"
BASE COUNT         1023 a         1779 c         1515 g          932 t
ORIGIN      
        1 gtggatccgt ccattgcgaa aattccgccc ggctcaggcc cgaatcagat cccggtcgcg
       61 gggtacaccc gcgtgtccgc cgacggcaag acggtgttca ccgtcgggta cgacgcgaca
      121 gcgggcaaga gcctgctctc cgccattgac gcgagcacca gcgacgccgt tctcggcgtg
      181 ctggatctcc ccgatatcgg cgtggccagt ttcgacgaga aggccggcga caggctgtat
      241 ctgcccagca acgacctcgg cgccaagaag aacgtgatcg cggtggtgga cgtcaatcca
      301 tcctcaccga cctatttgag cgtcatcggg gaaatcacgg tcggcgaagc gctcggcgac
      361 cgccacggcg aggtcagcac ggatggacgg gtggccgtgt acccggacac ctgcgccgcg
      421 tgcaacgcgg tcaatgtcat tgaaaccgcc acggatagcg tggtgacgac gctgaccttg
      481 ctcggaccac ccgccaaagc cgtcgggatg gtccggtggc cggtcaccgc gaccgagaac
      541 cattgatgga atgaaccgac gccttccgcg gtcataatta atcagacgtt cagtcgccaa
      601 ctaatcgcgt aacactttaa ccgcacggag cctgccccca tgtttcttgc cactcgcgcc
      661 gtttcgcagt cgacgtcttt gggacgctgt tttcggtttg ccgcttggct catcctgctg
      721 ggcgtgatcg cggggtgcgg cgggggcacg ggcggcgaca gcggctcgca agggctgatc
      781 ggcccggccg gaggaaccgt caccagcgct gacggcaagg tctccctggt catccccccc
      841 aacgccgtgg gggtcaccac gcaatttgcg attgatcccg atcccgcaaa cggcggcgcg
      901 ccatcgcccc gaattgtcgg gacggtctac agcatcgact ccgatccgac tgggatcagc
      961 ttcccagcca acgcgcttcc ccaattgacc atgccttatc caagcggggt ggcggatccg
     1021 tccaccctga cgatcgggac actcgaaaac ggccagtggg tgaacgtcgc gggatcgcag
     1081 gtcaaccagg cgaatcacac cgtgactgcg gcgctcgcgc atttgagccc ctatgcagtg
     1141 ctccaactgg ccagtggaac cgacaacaac ccgccgatcg ccagtgtcgg gggaccctac
     1201 caaggtgagg tcggacaatt aattcagttc gacgcggtcg gatcaatcga cccggatcgg
     1261 gatccgttga cgtacacgtg gaggttcggc gacggttcgc cggcggtcag cggtccacag
     1321 caatctgccg atcaccgcta tgcgacagcc gcaacattca cggtcaccct cacggtttcg
     1381 gacggccgcg gaggctccga cagcaaatcc acgaccgcga cgatcgtctc cgctcccccg
     1441 gtgaaccacc agcccacagc caatgctggc ggaccctatg ccgccacggt cggccaattg
     1501 atcacgttcg acgcctccgc gtcaaccgat cccgaccagg atccgttaac gtatgactgg
     1561 aacttcgggg acggcgccac ggccgccaac gccggtccgg cccctaccca cacgtacaac
     1621 accacgtccg gcagtccgtt cacggtcact gtcacggtca gggacccgtc cgaatcgttc
     1681 agcagcgcca ccgccacggc cacgatcagc cccttgccgc cggtcaacca cgcgccggcg
     1741 atcacgagca tgatgatgcc cactccctcc acggtcattg cgggacgctc catcctcgtg
     1801 gacgggacag ccaccgatgc ggacggcgat ctgctgacat atagttggaa cttcggcgat
     1861 ggatcgagca gcacgccggc gatctcccga tccgacgcca cccacacgta taacgccgcg
     1921 ggaccctacg gaccgtacga ggtcaaactg agcgtctccg acggccaagg cggagacgtc
     1981 ttcgatacgc ggaacctcgt tgttgaggcg cctccggtta accacgcgcc ggttgcacat
     2041 gccggcggtc cctatgtggg cgtggtcggc caaccgctcg cgttcgacgc ctccgcgtcc
     2101 agcgatcagg atcaggatcc gctcacctat tcctggacat tcggcgacgg cgcgacgggc
     2161 gacggcgtcg ccgtcacgca cgcatacagc accagcgcgg gcagcccatt caccgtgacc
     2221 gtgacggtca cggatccggg cgggttgtcc ggcagcgcct ccaccaccgc cacgattact
     2281 ctgcctccca accagccgcc gatcgccgtt gtgaatggac cgtatgccgg cacggtcggc
     2341 caggtcatta cgttcagcgc cgccggctcc gccgacccgg acaacgatcc cgcgaccctg
     2401 acatataact gggagttcgg cgacggcgcg acgggctccg gcttgtccac gactcacgca
     2461 tacacaaccg ccgccaccta tccggtcaag gtcacggtca cggacccggc tggcgcgtca
     2521 tcgagcgcct ccacgacggc agcgattacg gctcccactc ccgggaaccg acttcccgtg
     2581 atcagcgtca atgttccccc gcaggcgctc atttttcaac aagtagtctt gagtgggtca
     2641 ggatcggatc ccgacggcaa cacactgagt tacagttgga acttcggaga cggcaccaca
     2701 tcggccttcg tggccacggc gtcagccgcg caccaatacc agacagcggg aacctacact
     2761 ccgaccctga ccgccgacga tggccacggc ggagtcgttt cggtgtctgg atcagtgact
     2821 gtggtcgttc cggcacagcc ggtggcgttc ccagcgcaat taaaggtcta tatccgtcaa
     2881 aatgtccaag cgcctcaccg ttggcactac gcggtcccgt tgcatggcag cggagttccc
     2941 ccgctcacgt tttccatcgt cgcgtttccg acccaccaag atgcgagcct ccccaccatg
     3001 gcaatggcgg cgacccaata ttatcagtgg ctagatcccg tcacctcgga atggcgagtg
     3061 gaatgccaga gctcaacgcc tgcagccgcg tgtatggcga cgacgtcctc atcagtcaac
     3121 ctcgccaccg ggaccgtggt cgatccaaac acgagctccc ccatacaagt tccgtcgacc
     3181 ctgtcgccga ttgttgtgta cgcgccagca aactgcaacg atgccacctg ggggatcgga
     3241 cagtactcgg atgctttcgc gtttaccgtg acggacggca cgggcgtaac ctccgcgccc
     3301 gccaccgtga gcatctcatt ttttgatacc gggtgcgcgg gcggaacgca ttgatcattg
     3361 attacccagg atatttcgga tgattccggt tatcggagcc cacctccctg gaatgaagag
     3421 gacgaatccg tggtctttct gctcagcagt tcgtactcgg gcgtcccaat cacaggagtt
     3481 caataaaacg atgcgattcc cgcacgttcg gcaacgggtg atttttttcg tgagtgcggc
     3541 gctcttggcg aatagcgtct ttctgttgat gcggacggac gccgaagcca ttccggcgtt
     3601 tgcccgcaaa tacgacatgg actgtagcca ctgtcacagc atggcgccca agctcaatcg
     3661 ggtggggctg aagttccatg acaactttac gctcaaagag gcgctgggcg atttggaccc
     3721 cgaactgcgc gaccgcgtgc ggaatgaaga ccccgaagac atgcacccgg cgtattggcc
     3781 cgtgtcattc cgcattgccg gagggtacca gtacaacaaa cgagacaacc agccggtcga
     3841 ctcggccggc cttgaccttc ggactatcag caccaacacc tttgcgctcg aacgattcga
     3901 gctgctggcg ggaggtctgt tcgcccctgg catctcctat tacatttcgt attacccgga
     3961 ggccttgaac gtcggactcc caggccagcc tccagtccac gcgcaccccg gggcgacctc
     4021 gggctcgtca cagctcgggg ctctgggatt cgcctgggcc cggttcgccg acatcttcgg
     4081 ccagaccgcg caagacgaac atcacgatgc cgcggccgac ggccacgacc atgaggccgc
     4141 cgaacccaaa cacgatcacg ggcaggatct gattctcggc agtcacgaac tccatctgac
     4201 cctttcaggc caccatcgac tcaccaacgc accgtatctg gcgtatcgct acgacccgct
     4261 ctacgtgctg gacccagcga agggattcaa actggatgcc ccgcaactcg gagcctcact
     4321 ggacggctcc acgccgtggt tcggctacgc ggtttcgctt tataacggca ccagcaactc
     4381 ggccgacgat aaccgcacct tggacgtgtt cgccacgatc aaccaagact tcggcgacaa
     4441 tcgggtcgga gtgttcggtc tgcgcggcac gtcgcccacg agttggatca tgaccaccac
     4501 cacatcgccg ccgtcccctg ttccgggaac cgggagcgcc aacaaaccgt acttccgatt
     4561 cggcgccgac accgacctca atgtcgggcc cctgaacgtc ctgttcttcg cgctgtatgg
     4621 gcaggtcgat aaggatctgt tcgatccgtc gctcggccaa accgcgaagt tttacgcggg
     4681 cttcctggaa acagactacc tgatcgaatc cgtgcgcacc atgctgatcg cccgctacga
     4741 cggcgttcgc aacgtctccc aagggctgtc gacgtacgcg cacaaccggg gggacactga
     4801 cgcgttcacc ctggcggtcc gacgcgacct ggtcttgacc agccgcgcga accttcaagt
     4861 ccatgtcgaa gccaacgcca cgcgcgtcca agccatcgct accaacgaca acgatcagac
     4921 atccaacacg ttctttgccg gcgtggactg gtctttttag caaatgattc ggtggttcga
     4981 gggttcaagc gacacaaacg gaaaggacac gaaatgagca cttcggcctt cggatggacg
     5041 agagcagccg cggcggcatt cgctttggcg gccgccggct gcgcgagcgg cagcgctccg
     5101 gtcgacgggc aatccggcgg gtccacatcc gacgtgttca ccattagcgg gagtctcacg
     5161 acacccgtcg gcgtcacgct gggacccggc gcgtccggca gcatcacgtt ctcggtcaag
     5221 ggaagcgatc cggcatcgac gttttactg
//
LOCUS       DBNGTT010000450         6101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2661659, whole genome shotgun sequence.
ACCESSION   DBNGTT010000450 DBNGTT010000000
VERSION     DBNGTT010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2661659"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..135
                     /locus_tag="AC16BC_10195"
     CDS             4..135
                     /locus_tag="AC16BC_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242029.1"
                     /translation="MTAILDGWREHGTKWLPEYQAGTCGPVEADRLIERDGRRWRTL"
     gene            155..1147
                     /gene="glk"
                     /locus_tag="AC16BC_10200"
     CDS             155..1147
                     /gene="glk"
                     /locus_tag="AC16BC_10200"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR3242030.1"
                     /translation="MILAGDIGGTKTVLALVRPTASGRDIEREAVFASREYGRFDDLL
                     AAFLKEGGHRPDAVCLGVAGPVREGRCQTTNLPWVLEERELGRAVGARAVKLLNDVEA
                     AAFGVLGLRADEVVALNPGRRAVGNIAVIAAGTGLGEAILAWDGRRYVPVPSEGGHTD
                     FAPRTDREIDLLRFLRAEFGRVSYERVVSGPGQYNIYRFLRDTGTVEEPAWLTSELAA
                     GDPSAVVTRVGLAKGHPLCVDALDLFAALYGAEAGNLALKAFAIGGVFIAGGIAPKMI
                     DKLRDGVFMAAFAAKGRYRSLMESIPVSVSLNPRTPLLGAARYAVEHLVSAAAS"
     gene            1368..1718
                     /locus_tag="AC16BC_10205"
     CDS             1368..1718
                     /locus_tag="AC16BC_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3242031.1"
                     /translation="MPKDQIDAAKAARNPFPPSPENVAKGDGLYHGKGTCFVCHGQAG
                     QGDGPAAVGLDPSPRNFTNAAFHQARTDGELMWVLKNGSPGTAMVPLVGSVIDEQEGW
                     FIILYERSLAQPGK"
     gene            1774..2130
                     /locus_tag="AC16BC_10210"
     CDS             1774..2130
                     /locus_tag="AC16BC_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242032.1"
                     /translation="MRCSRGVRIGLVALVATVVSTFTTVGITRGADGRVAVVSPKPNE
                     VVGTDVTVTWTQEKSGRADHVHVFVDRGHMKPVITGTSTVIKGLSKGVHEITVQAYSS
                     DHEPLDAKASVTVKVE"
     gene            2181..2627
                     /locus_tag="AC16BC_10215"
     CDS             2181..2627
                     /locus_tag="AC16BC_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3242033.1"
                     /translation="MGKSTGVLVAGVVALAFATGVAVAAEKDIKIPRVPAGELQKAKD
                     MKNPLKPTPDVLAKGKAIFEGKGTCFTCHGQTGKGDGPAGAALDPSPRNFTNPEFNKV
                     RTAGEMFWVVKNGSPGTGMISYNPAMINDEEAWEAITYARSLGGEK"
     gene            complement(2748..4763)
                     /locus_tag="AC16BC_10220"
     CDS             complement(2748..4763)
                     /locus_tag="AC16BC_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3242034.1"
                     /translation="MVLIAIGYANSLNGEFHLDDLTFKEDATLHLTDLKLDSLASVVW
                     HRRPVASSTFALNYYWGGHEVFGYHMVNVAIHLAAALAWYAFLVIALGLPRAQPLFQN
                     REAAAALAATLLWAIHPVQTQAVSYVVQRMTTLASLFYVLALVAYLKGRLSAGMRRWV
                     WWAIALASAALALGSKENAATLPVAVLLIEVCLMSGLRGLRWRTVMAWTVGLAVPVLI
                     VAIFAAQHEAGGSFLTQLTAHRSTTQWFSVGERLLTEGRVIIHYIGLLMFPHPSRLTF
                     DYAFPISHSWLDPPTTVLAWAAIAAALTWALWSVRRTPVASFCVLWFFLNLAVESTVI
                     RLDLVFEHRLYLPSMGAALGAVALGMTIGRRLGAWGRSLAPIACAVLLILWTSWTIER
                     NRVWATDFSLWADTAAKAPANPRALTLLGIAYTDHGEADKALEAFQAAARANPDFHDA
                     HANLGSALIGRGDLTGALEAFTRARALDPNNAEDAYHLGLIYYKLGRMEEAQREYEAS
                     LQLDPEDAETHNNLGAVHQAAGRWEAAKAEYESALQRNPELAAAHYGLGQVYHHLGRE
                     GEAIQSFQETIRGNPTHLDAHLALAGIYLGRDDKPAALVWFQAALQLAPTSPQGHFQV
                     ARLLDQLGRRSEALAAYRQFVALAASDQAQTRSWAQERIRALEARQG"
     gene            5807..>6101
                     /locus_tag="AC16BC_10225"
     CDS             5807..>6101
                     /locus_tag="AC16BC_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HIR3242035.1"
                     /translation="MTAQRVYRSLTSGGPYTQITSFANNTTSTYTDTGLTNGTTYYYV
                     LRAFDGTQESADSNQASAVPLDNTAAAAPTGLSAADVAGDNGGALALSWTPSVS"
BASE COUNT         1131 a         1811 c         2066 g         1093 t
ORIGIN      
        1 tgggtgactg cgatcttgga cggctggcgc gagcatggga cgaagtggct gcccgagtac
       61 caagccggga catgcggacc ggtcgaggcc gatcggttga tcgaacgcga cgggcgccgt
      121 tggcgcacgc tctgatcgtc cacggcacgc gcccatgatc ttagccggag acatcggcgg
      181 gacgaaaacg gtgctcgcgc tcgtgcgccc gaccgcctcg ggtcgggaca tcgagcgaga
      241 agccgtcttc gcgagtcgcg agtacgggcg gttcgacgac ctgctcgccg cgttcctgaa
      301 agaagggggt caccgtccgg acgcggtctg tctcggcgtg gcgggtccgg ttcgcgaggg
      361 gcgttgccag acgacgaatt tgccgtgggt gctggaggaa cgtgaattgg gtcgggcggt
      421 cggcgcccgc gccgtgaaat tgctcaacga cgtggaggcg gccgcgttcg gcgtgctcgg
      481 tttgcgggcc gatgaagtgg tggcgctgaa tcccgggcgc cgggccgtgg gcaatatcgc
      541 cgtgatcgcg gccggtaccg ggcttggcga ggccattctg gcgtgggatg gccggcgcta
      601 cgttccggtg ccttccgagg gggggcacac cgactttgcg ccgaggaccg accgcgagat
      661 cgatttgctg cgattcctcc gggccgagtt tgggcgcgtc agttacgaac gagtcgtgtc
      721 gggcccgggc caatacaaca tttatcgatt tctgcgggat accggaaccg ttgaagagcc
      781 ggcgtggctg acaagcgaac tggctgccgg cgacccctcg gcggtcgtga cgagggtggg
      841 gcttgcgaag gggcatccgc tctgcgttga tgcgctggat ctgtttgccg cgctctatgg
      901 agccgaagcc ggaaacctcg cgctcaaagc gttcgccatc ggcggcgtgt tcatcgccgg
      961 aggcatcgcg ccgaaaatga tcgacaaact tcgcgacggc gtcttcatgg cggcctttgc
     1021 cgcgaaaggc aggtaccgct cgctgatgga gtccattccc gtgagcgtgt ccttgaatcc
     1081 ccggacgccg cttctgggcg ccgcgcgtta cgcggtcgag cacctggtct cggccgcggc
     1141 atcctagccg catctcgtcg tccgccggtg tcgcgttcga ccgcttccat tgacgcgtcg
     1201 gcttgacaag ggggggcgat ttctttaaga tccccgccgc attgaaacac cgaagacgat
     1261 tgcacgatag gtatggtatg tcgtggcagc tgcgggtggc gtgtctatta gtttgcttgc
     1321 tccccacggc agcgctgtcg gctgaacgcg atccttcgca accccgcgtg ccgaaggacc
     1381 aaatcgatgc cgccaaggct gcgagaaacc cctttcctcc ttcgccggag aatgtcgcga
     1441 agggcgacgg actgtatcac ggaaaaggta cgtgttttgt ctgccacggc caggcgggac
     1501 aaggtgacgg tcccgcggcg gtcggcctcg atccctcccc gaggaacttt accaacgcgg
     1561 cgtttcacca ggcgcggacc gatggggaac tcatgtgggt gctgaagaac gggagtcccg
     1621 gcacggccat ggtccccctg gtgggaagcg tgatcgacga acaggaaggg tggttcatca
     1681 tcttgtacga acggagcctg gcccaacctg gcaagtgagt ggcgcgatca atgaatattc
     1741 cagcccgatt tccgtctcaa gaggcgaccc tgagtgaggt gtagccgggg ggtgcggatc
     1801 gggttggttg cccttgtggc gaccgtggtg tcgaccttca caacagtcgg catcacccga
     1861 ggggctgacg ggagggtggc ggtggtgtcg cccaaaccga acgaggtggt ggggacggac
     1921 gtgacggtga cgtggacgca ggagaaatcg ggacgagccg accacgtgca tgtgttcgtg
     1981 gatcggggcc acatgaagcc ggtgatcacc ggcacgtcga cggtcatcaa ggggttgtcg
     2041 aagggagtgc atgaaatcac ggttcaggcg tacagcagcg atcacgagcc gctcgatgcc
     2101 aaggcgtccg ttaccgtgaa ggtcgaatag accaaggcag tcaggggctc actatcattc
     2161 atcaggagga ggagcgttcc atggggaaga gcacgggagt gttggtcgca ggcgtggtgg
     2221 cgttggcgtt tgcgacgggc gtggcggtcg cggcggagaa agacatcaag attccgcgcg
     2281 ttccggccgg tgagttgcag aaagccaagg acatgaagaa tccgttgaag ccgacgcccg
     2341 acgtgctggc gaagggcaag gcgattttcg agggtaaggg aacctgcttc acctgccacg
     2401 gccaaacggg taagggtgat ggccctgccg gggcggctct tgatccgtct ccccgcaact
     2461 tcaccaatcc ggagttcaac aaggtccgca ccgcgggcga gatgttctgg gtggtcaaga
     2521 acggcagccc gggcacgggg atgatctcgt acaaccccgc gatgatcaac gacgaagagg
     2581 cgtgggaagc gattacctac gcccggtcgc tcggcggcga gaaataacac gcttcatcta
     2641 acgtagtttt cgcgtagttc gaatagcgat tgagggaggc ccggacaacc gggcctccct
     2701 tttttggggg gggcgctcgg aggccggacg ggcgatgcgg gtgcgtttta gccctggcgg
     2761 gcctccaaag cacgaatacg ctcttgagcc catgagcggg tttgagcctg atcgctcgcg
     2821 gccagcgcga cgaactggcg gtacgcggcc agcgcctcgg agcggcggcc gagctgatct
     2881 aggaggcgcg cgacctggaa gtgtccttgc gggctggtgg gagcaagctg taaggcggct
     2941 tgaaaccata ccagagctgc gggcttgtca tctcgcccga gatagatccc cgcgagtgcg
     3001 aggtgggcgt caaggtgcgt cgggttccct cggatggtct cctgaaatga ttgaatggcc
     3061 tcaccctctc tcccgagatg gtggtacacc tgcccgaggc cgtagtgggc agcggcgagt
     3121 tcggggttgc gttgcagggc ggactcgtat tctgctttgg ccgcctccca tcggccggca
     3181 gcctggtgca cggcgccaag attgttgtgt gtctctgcgt cttccgggtc gagctgcagc
     3241 gaggcctcgt attctcgctg cgcctcctcc atgcggccga gcttgtagta gatcaagccg
     3301 aggtgatagg cgtcctcggc gttgttggga tcaagcgcgc gtgcccgagt gaaggcctcc
     3361 agcgcgccgg tcaggtcccc tcggccgatc agcgcgctgc cgaggttcgc gtgcgcatcg
     3421 tggaagtcgg ggttcgcccg cgccgcggcc tggaaggcct cgagagcctt gtcagcttcg
     3481 ccgtgatccg tgtaggcgat acctaagagg gtgagcgcac gggggttggc cggcgccttg
     3541 gctgcggtat ccgcccagag cgaaaagtcc gttgcccaca cacggtttct ctcgatcgtc
     3601 caagacgtcc acaggatcag gagcaccgcg cacgcgatcg gcgccaggga tcgtccccag
     3661 gctcccagcc gccgaccgat tgtcatcccc agcgccaccg cccccaatgc agcgcccatc
     3721 gacggcaaat agagccggtg ctcgaacacc agatcgagcc ggattaccgt ggattcgacc
     3781 gccaggttga ggaaaaacca caagacacag aacgacgcga ccggcgttcg tcgaacggac
     3841 cagagcgccc atgtgagggc ggctgcgatc gccgcccacg ccaacaccgt agtgggtgga
     3901 tccaaccaac tgtgcgaaat gggaaacgca tagtcgaatg tgagtcgcga tgggtgcggg
     3961 aacatcagga gaccgatgta gtggatgatg acgcgcccct cggtgagcag gcgctcgccc
     4021 acagagaacc attgggtcgt cgaacgatgc gcggtgagct gcgtcaaaaa tgagccacct
     4081 gcttcgtgtt gggccgcgaa gattgccacg atgagaaccg gcacggcgag ccccacagtc
     4141 catgccatca ctgtccgcca ccgaagtcca cggaggccgc tcatcagaca cacctcgatg
     4201 agcaggacgg cgacaggcag cgtcgcggcg ttttctttac tgcccagtgc gagggccgcg
     4261 cttgcgagcg cgatcgccca ccagacccac cggcgcatac ctgcgctgag ccgacccttg
     4321 aggtacgcga ccagcgcgag gacataaaag agcgaggcca gggtcgtcat gcgctggacc
     4381 acgtagctca ccgcctgcgt ctggactggg tggatcgccc agagcaacgt ggccgccaac
     4441 gcggctgcag cttctctgtt ttggaacaac ggctgcgcgc gagggaggcc cagcgctatg
     4501 accaggaagg cgtaccaggc cagcgccgcg gcgagatgta ttgcgacgtt gaccatgtga
     4561 tagccgaaca cctcgtgccc gccccagtag taattcaacg cgaaggtcga gctggctacc
     4621 ggccgtcgat gccagacgac gctggcgagt gaatcgagct tgaggtccgt gaggtggagc
     4681 gtggcgtcct ctttgaaggt gagatcgtcc aggtggaact cgccgtttag gctgttggcg
     4741 tagccgatgg cgatcaacac cacgatcgcg gtgaccgcgg acgcgtgcca gcgccacttt
     4801 gatggaggga cggatggggt ccgttcgcgc acctgatggg aacgcttgtg ccgaggagcc
     4861 gaaggcgatc gagttgccaa gtcagctgac acgatagtca ttccgtcgcg gctcagtcaa
     4921 gtctgtagcc cccaaagttc gtcttggtag gggttcattt ctgtccaatc tctcgttttt
     4981 tcacagaatg tcaaggctcc tctcctggtc ttgtacgagg gaaagggagg gtccgccatg
     5041 atgacgaggg gaaaatttcc cctttttcgg cttctacagc atgcgtggcg ggccaccacg
     5101 cccacgaccc gtcagggacg ttcgatccta atccattgaa aaaccgtctc ttttaccgtg
     5161 gaggggataa agtgaccctg tggagggggt ccgtgcgagc acggactttg cgccactggg
     5221 gtcatttggc tgaactaagc gattggttcg cctcctttct gttgtctccc aagacacttc
     5281 aggcggaatc agacgcgctc gcatgatccc atcctttctt acctcctgca ccaggctgag
     5341 cggagcatac aagcagagct ggatcgtgct ggcagtcgcg gtgagttggc tgggcctcgc
     5401 tccgtggacc gcgctcgcag cagagccacc gaccagcctg actgcgactg atacgcccaa
     5461 cgacgctggg ggttcgatca gcctgacttg gacgccgtca atctcggctg gtgtgaccga
     5521 gcaacgactc taccggagtt tgacctccgg cggcccctat acgctggcca ccacttttac
     5581 agacaacacc accagcgcgc acaccgagac aggcctcacc gatgggacaa cctattacta
     5641 cgtcatccgg gcgttcgatg ggatcgaatc agcggattcg aatcaagcct cggcggcccc
     5701 gctggacaac cggccggcgg ccccgacagg gctgagtgct tcggatgtag ctggcgacaa
     5761 cgggggcgca atcgctctga catggacacc atcgagttcc gggacggtga ccgcgcaacg
     5821 ggtgtatcgc tcgctcacca gcggcgggcc gtacacccag atcacgagct tcgccaacaa
     5881 cacgaccagc acgtacaccg acacgggcct gacgaacggg acgacgtact actatgtgct
     5941 ccgggcgttc gacgggaccc aggagagcgc cgatagcaat caagcctcgg cggtcccgct
     6001 ggacaacacg gcggccgcgg cgcccaccgg gctgagcgcg gcggacgtgg ccggggacaa
     6061 cgggggggcg ctggccttgt cctggacccc gtcggtgtcc a
//
LOCUS       DBNGTT010000451         4827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_361451, whole genome shotgun sequence.
ACCESSION   DBNGTT010000451 DBNGTT010000000
VERSION     DBNGTT010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_361451"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="AC16BC_10230"
     CDS             complement(<1..743)
                     /locus_tag="AC16BC_10230"
                     /inference="COORDINATES: protein motif:HMM:NF016593.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR3242036.1"
                     /translation="MYHPTFEEFTATARRGNLIPVYKEILADLETPVSAFLKLDDGRY
                     AYLLESVEGGEQWARYSFLGSRPTVVIEARGPRVEIRRGDRIEVVETDDPLRAVEKVM
                     ADYTPVAVPGLPPFVGGAVGYIGYDVAPRSFEHLAMRGKPSLGLPDLCLLITDTLVIF
                     DNLAQKIKVVSNAHVTGRPLREVYDESVAKIDAMIAALRRPLTPPTPQPARPHPAPAS
                     NVTKPEFVRMVRRAKEYIAAGDVFQVVLS"
     gene            complement(854..1051)
                     /locus_tag="AC16BC_10235"
     CDS             complement(854..1051)
                     /locus_tag="AC16BC_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242037.1"
                     /translation="MGEHSKVNGSARQIQVMVPTALYDELMTALGERDINDAVLDSLK
                     ETLRKLRFKRDLERGAPRRKA"
     gene            1248..1970
                     /locus_tag="AC16BC_10240"
     CDS             1248..1970
                     /locus_tag="AC16BC_10240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3242038.1"
                     /translation="MDPRIADLQALITGANRVTALTGAGISAESGIPTFRGPEGLWNN
                     YRPEELATPEAFARDPELVWRWYDWRRGIVAKAKPNAAHTGLAALQQRQAVTVITQNV
                     DGLHGKAGSRELIELHGNIWKVRCTSCAVIATNTDVPLATIPPSCKSCGALVRPHIVW
                     FGESVDPYDFQRSVEMSRSADVFLVIGTSGLVQPAASFAQIAKSAGAAVVEINPDDTP
                     TTPIADLAFRERAVPVFEALGL"
     gene            2419..>4827
                     /locus_tag="AC16BC_10245"
     CDS             2419..>4827
                     /locus_tag="AC16BC_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HIR3242039.1"
                     /translation="MFGGLPAPDGLYHPAQERDACGIGFVADIKGRKSHDIVRKAIEV
                     LNNLEHRGAQGCDPCTGDGAGILLQIPHEFLARVAPQSRIALPRAQEYGVGMVFLPQD
                     ANGRRLCEQIIERVILEEGQRFLGWRDVPVKESELGWLARQSMPAIRQIFVARDILSE
                     ADFERKLYVIRKRIESSIRDSAIPGREEFYVPSLSCRTIVYKGLLLPNQLEKFYQDLG
                     DGTLTTAIGLVHSRFSTNTFPTWPLSHPYRYVCHNGEINTLKGNVNWMRARQGRLQSD
                     LYGDDIDKLFPIIYENQSDSACFDNALEFLVMGGRSLPHAMMMLIPEAWVGNPQMDLD
                     RRGFYEYHAAIMEPWDGPAAMAFTDGRLIGGTLDRNGLRPARWLITHDGLAVLASEAG
                     VLTFAPQEIRAKGRLQPGKMFLVDTVEGRIIDDEEIKSDIASRKPYREWVATNRLSLD
                     ELPQPMSVPQPDHATLRQRQQAFGYTVEELKMVLTPMVVNGEEAVGSMGTDTPLAVLS
                     ERPQLLFKYFKQLFAQVTNPPIDPIREALVMSLVTNIGPKPNLLSETPETCRRIKVQQ
                     PILSNFDLQKIRSITDPDFRSRTLKMLFRVAEGPDGLAGAVEDLCRQAAEAVRAGDKF
                     LILSDRGVNEEWAPIPSLLAIAAVHHHMIRESLRAEVGLTVETGEAREIHHFACLIGF
                     GAGTVNPYLAFETLVDMARDAYLPEGIDAATAESKYIKAVNKGLLKVFSKMGISTVQS
                     YCGAQIFEAIGIGSDVINRYFTGTASRIQGIGLREIAEEVLRRHGQAYAPAPVHQLDF
                     GSEFH"
BASE COUNT          891 a         1632 c         1514 g          790 t
ORIGIN      
        1 tgggacaaca ccacctggaa cacgtcgccc gccgcaatgt actccttggc ccgccgcacc
       61 atccgcacga actcgggctt cgtgacgttc gaagccggcg ccgggtgggg ccgggccggt
      121 tgcggcgtcg gcggggtcaa gggacggcgc aacgccgcga tcatggcgtc gatcttggcg
      181 acggactcgt catagacttc gcgcagcgga cgcccggtca cgtgcgcgtt ggacaccact
      241 ttgatcttct gcgcgaggtt gtcgaagatc accagggtgt ccgtgatcaa cagacagagg
      301 tcgggcaacc cgagcgaggg tttgccccgc atcgccaggt gctcgaacga gcgcggggcc
      361 acgtcgtacc cgatgtaccc gaccgcgccg ccgacaaacg gcggcaaccc cggcaccgcc
      421 accggcgtgt agtccgccat gaccttctcg accgcccgga gcggatcgtc cgtctcgaca
      481 acctcgatcc gatcgccgcg tcggatttcg acgcgcggcc cgcgcgcttc gatcaccacc
      541 gtggggcggc tgcccaggaa ggaataccgc gcccactgct cgccgccctc cacgctttcg
      601 agcaggtacg catagcggcc gtcgtcgagc ttcaagaacg cggacacggg cgtctcgaga
      661 tcggccagga tctccttgta gacggggatc agattcccgc gacgggcggt cgcggtgaac
      721 tcttcaaagg tggggtgata cacgtcggga tacgccgcgg ttaggcggaa tgagccgtta
      781 ccgtaacacg gcgcgagcgg gcctgtcaac ccacgtgcgg tataccgccg cagcacgcgg
      841 cgggacggac ggtttacgct ttccggcgcg gcgcgccgcg ttcgaggtcg cgtttaaacc
      901 ggagcttgcg aagggtttct ttcaacgaat cgagcacggc gtcgttgata tcgcgctctc
      961 ccaacgccgt catcagttca tcgtagagcg ccgtgggtac catcacctgg atttgcctgg
     1021 ccgagccgtt caccttgctg tgttcaccca tccctgtcct cccggcttgc gcccgcgggg
     1081 tcgcgttgga gttcggctgt atgttggcgt ttgtgcagcc gaacagtgta accgccaacg
     1141 tccaatgaat attcccccgt attttttcgc gagacgcatg tactatactg gggcctcatg
     1201 gctcggcacg ggaccgggca tcgcccccat tggtattaat cgcatccttg gacccgagaa
     1261 tcgcagacct tcaggccctt atcaccgggg cgaaccgcgt cacggcatta accggcgcgg
     1321 gcatttccgc ggaaagcgga atcccgacgt tccgcggccc cgaaggcctg tggaacaact
     1381 accggccgga agagctcgcc acccccgaag cctttgcgcg ggacccggag ctggtgtggc
     1441 gatggtacga ctggcggcgc gggatcgtgg ccaaggccaa accgaacgcg gcgcacaccg
     1501 gattggctgc gctccagcaa cggcaggccg tgaccgtgat cacccagaac gttgacggac
     1561 tccacggcaa agccggcagc cgcgaattga tcgaactgca cggcaacatc tggaaagtac
     1621 gctgtacgag ctgcgccgtg atcgcgacca acaccgacgt gccgctcgcg accatccctc
     1681 cgtcgtgcaa gagctgcgga gcattagtcc gcccgcacat cgtgtggttc ggcgagtccg
     1741 tggacccata cgactttcag cgcagcgtag agatgagccg cagcgcggac gtatttctgg
     1801 tgatcggcac ctcgggtctg gtccagcccg ccgcctcgtt cgcccagatc gccaaatccg
     1861 ccggcgccgc cgtggtggaa atcaaccccg acgacacccc caccaccccg atcgccgacc
     1921 tcgcgttccg cgaacgcgcc gtccccgtgt ttgaggcttt gggcctttag tgcttccgcc
     1981 gacgacactg gccgacgaaa taatggcgga tgcggcctcc aaccacacct aacccgccct
     2041 gaatccttcc gtgtcgcgaa agcgacacga ggctgttcag gagcggtcag atgcaaggcg
     2101 ccgcgagaac cggaccggag cgtactctgc gtgtacgtga gggcgagtgg cgcgacgaag
     2161 aaggagcgcc acgccacgag cgccgccgag gcggtgaggc ggccgggcac ggaagcgcag
     2221 cggcaacgac gcagatggcc ggtcatggac agcctccacc ccacaccggc atagcgcttg
     2281 acaccacccc gccaattccg ttaggttgaa ggaccctccg ggtggtacat ccaaccgcga
     2341 gtaggcttcc agatggcatc ggatagaaga tcggcaagcg tcggcccggc ccaagagcca
     2401 gatccggcga attcacccat gttcggcggt ctgcccgctc ccgacgggct gtatcatccc
     2461 gcgcaggaac gcgacgcgtg cggtatcggc ttcgtcgcgg acatcaaggg ccgcaagtcc
     2521 cacgacatcg tgcgtaaggc gatcgaggtg ctcaacaacc tcgaacaccg cggcgcgcaa
     2581 ggctgcgacc cctgcaccgg ggacggagcc ggcatcctgc tgcaaatccc ccacgaattc
     2641 ctggcgcgcg tggcgccgca atcgcgcatc gccctgccca gggcgcagga gtacggggtc
     2701 ggaatggtgt tcctcccgca agacgccaac ggccgccgcc tgtgcgaaca gatcatcgaa
     2761 cgggtcattc tggaagaggg ccagcgattc ctgggctggc gcgacgtgcc ggtcaaggag
     2821 tccgaactcg gctggctggc gcgccaatcc atgcccgcga tccggcagat cttcgtagcg
     2881 cgcgacatcc tgagcgaagc ggacttcgaa cgaaagctct acgtcattcg caagcgaatc
     2941 gagtcgtcga tccgcgattc cgcgatcccg ggacgcgagg agttctacgt tcccagcctg
     3001 tcgtgccgga ccatcgtcta taaagggctg ctgttgccca accagctcga aaagttctac
     3061 caggatctgg gcgacggcac gctcaccacc gcgatcggcc tggtgcattc gcggttctcg
     3121 accaatacgt ttcccacctg gccgttgtcg cacccctacc gctacgtgtg ccacaacggc
     3181 gagatcaata cgctcaaggg caacgtcaat tggatgcgcg cgcggcaagg ccggctccag
     3241 tccgacctgt acggcgacga catcgacaaa ctcttcccga tcatttacga gaaccagagc
     3301 gattcggcgt gttttgacaa cgcgctcgaa tttctcgtca tgggcggccg ttccctgccg
     3361 cacgccatga tgatgctcat ccccgaggcc tgggtgggca acccgcagat ggacctcgac
     3421 cgccgcgggt tctacgaata ccacgccgcg atcatggagc cctgggacgg gcccgcggcc
     3481 atggcgttca cggacggccg gctgatcggc ggcacgctcg accgcaacgg cctgcgtccc
     3541 gcgcgctggc tgatcacgca cgacgggctc gcggtgctgg cgtccgaagc cggcgtgctc
     3601 acgttcgcgc cccaggagat ccgcgccaag ggccggttgc agccgggcaa gatgttcctg
     3661 gtggacacgg tcgagggccg gatcatcgac gacgaagaga tcaagagcga catcgcgtcg
     3721 cgcaagccct atcgcgagtg ggtcgccacc aaccgcttgt cgctggacga gttgccccag
     3781 cccatgagcg tgccgcagcc cgatcacgcc acgttgcggc agcgccagca ggcgttcggg
     3841 tatacggtcg aagaactcaa gatggtgctc accccgatgg tggtcaacgg cgaggaagcc
     3901 gtgggctcga tgggcaccga cacgccgctc gcggtgttgt ccgagcggcc gcaactgctc
     3961 tttaaatact tcaagcaact cttcgcgcag gtgaccaatc cgccgatcga cccgatccgc
     4021 gaagccctgg tcatgtccct ggtgaccaac atcggtccca agccgaatct gctgagcgag
     4081 acgccggaga cgtgccggcg catcaaggtc cagcaaccca tcctgtccaa cttcgacctg
     4141 cagaagatcc gctcgatcac ggacccggac ttccgcagcc gcacgctcaa gatgctgttc
     4201 cgcgtggccg agggcccgga cgggttggcc ggcgcggtgg aggacctttg ccgccaggcc
     4261 gcggaggccg ttcgcgcggg cgataaattc ctgatcctga gcgatcgcgg cgtcaacgaa
     4321 gagtgggccc cgattcccag cctgctcgcc atcgccgcgg tccaccacca catgatccga
     4381 gaatcgctcc gcgccgaggt gggcctcacc gtggaaaccg gcgaggcgcg cgagatccac
     4441 cacttcgcgt gtttgatcgg gttcggcgcc gggaccgtca acccctatct cgcgttcgag
     4501 accctggtgg acatggcgcg cgacgcgtac ctgcccgaag gcatcgacgc ggccaccgcc
     4561 gagtccaagt acatcaaggc cgtcaacaag ggcctgctca aggtcttctc caagatgggc
     4621 atctccacgg tgcagagcta ctgcggcgcg cagatcttcg aagccatcgg catcggttcc
     4681 gacgtgatca accggtactt cacgggcacg gcctcgcgca tccagggcat cgggttgcgc
     4741 gaaatcgcgg aagaggtgtt gcgccggcac ggccaggcct atgcgcccgc gccggtccac
     4801 cagctcgatt tcggcagcga gtttcac
//
LOCUS       DBNGTT010000452         4364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1129252, whole genome shotgun sequence.
ACCESSION   DBNGTT010000452 DBNGTT010000000
VERSION     DBNGTT010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1129252"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="AC16BC_10250"
     CDS             <1..1668
                     /locus_tag="AC16BC_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242040.1"
                     /translation="THHFVGGNNYMTKLVGADAAANGDEVAPYPELLHTSFSSTHHDS
                     RFHSARFDTTKIMPGSMPKTGPETQHERMAWDRMRNVNNLTLSVQASVNKPTTEILVP
                     ITAALTNDGSGHNFPTGFPEGRVAWVRVKAWDTKNTANIADDVELQIQNAAYGNRRSL
                     GVGYLTAAPMEDPNFPGCHWEIPEGSPDPYAFVMKAVSSLDKMCPTLDLPYATPINLV
                     VNASNMPIDQNGVVIDRANPLGIPRYLDLDGDGDFFDDSFLSDTRLRPLPHTDATKQL
                     NHYSIVVPSTVVGPIAVTMASYFQAFEGIVAKKLLGNLANTDDADANEGFPGGTPTLE
                     PCVLNGPCDRVGKAGATGEAQIREALKYDPVVVEGAPPTPMEVKNAVIQVSGTTDNVA
                     PRLIINNSKNNPNTTAAIPANRHWSPSPYGGATGNYAAEGMGEMNVSWRRVVKVTFTE
                     PVRECVGSTCGPLTAKTFYLTDDAGNMVTALVDQIDDTTWALFPYLDDMLKPTFLDGK
                     TYVINVAPSVSTGGATYKIVDFAGNQLQAGPASAGKYTFGFSAMARP"
     gene            1796..4129
                     /locus_tag="AC16BC_10255"
     CDS             1796..4129
                     /locus_tag="AC16BC_10255"
                     /inference="COORDINATES: protein motif:HMM:NF022747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR3242041.1"
                     /translation="MRQFISGMLLGVVLFFFLSFLGRSTAGAVEQHTMAYVANAGNNH
                     VQVIDVDSGRTLTKLYTGATPWRLRLAPDAKTLWVQHWYAQTTAVVDLKTQSVSAILP
                     ERGPGIFNAKGNLFWSFSWPESTLEAYDTKRFKPVRSRETNAKVPYDLALAWKDSSLF
                     VGEYDPVSKGNRRVYDFLRAYSMKDDKAPGVSLSLGDSPARMRLDPTGRYLVVANLDG
                     EELSLVNQNNAKAKITLVPSPQDILFGPHGLMVVLAWKPERRASEIVTLQVDWSKLPW
                     PEVTPSASKEIQGGLVAGQMDPSGKRFYALDRPGRRLVVFDLPALNEIKSIPVGDVPY
                     DLVVTQVNRKQRDRWTEERLSQTRLKRIIENMKAQGKAYQDLQFVEEMRLPQLPPEQE
                     VESGAPEEKTDKTAEPTTTKIQVRFQAPDSLRIEYEDGGIRLAQGGKAVTITPEGRFF
                     DSPRQDLLYVLYALHSLPVEDVIRHLAGDVPGSPFLKNGIAVDVLGQVKENGHVYDVI
                     GTQQSGARVSQLWVDANRSTPVSFVEQFPVMEAQSPHQKAGTLSGIAETKLQYEKRQD
                     GYQVPVRMVRVVDGLETGRVTLSDVRVNPGLQSAQFDLARLGVVAVTASEPHHAKTVA
                     SKHEPGRVVPGMGNAHIDHPAAPHDPYNSNPPTSGTHTRYLTDWGIHKIPIPPETQVH
                     NLEDGGVLLQYNCPQGCVEWVTRLEQLAMKNDKVVVAPYPLMKAKLALTAWERIEILD
                     QFDEGRVTAFINAYAGKDHHPEGGEGAVADPLGKAGE"
BASE COUNT          909 a         1308 c         1326 g          821 t
ORIGIN      
        1 acccatcact tcgtgggcgg caacaactac atgacgaagc tggtcggcgc ggacgcggcg
       61 gccaacgggg acgaggtcgc gccctatccg gaattgttgc acaccagctt ctcctccacc
      121 caccacgaca gccgcttcca cagcgcgcgg ttcgacacca cgaagatcat gccgggcagt
      181 atgcccaaga cgggtcccga aacccagcac gaacgtatgg cctgggaccg gatgagaaat
      241 gtgaacaacc tgacgctttc cgttcaggcc tctgtgaaca agccgacgac cgagatactg
      301 gttcccatta cggcggccct caccaacgac gggtccgggc acaacttccc caccggattc
      361 cccgaggggc gcgtcgcgtg ggtgcgggtg aaggcctggg acacgaagaa caccgcgaac
      421 atcgcggatg acgtggaact gcagatccag aacgcggctt atgggaatcg gagaagcttg
      481 ggcgtaggct atttgaccgc cgctcccatg gaggacccca atttccccgg ctgtcactgg
      541 gaaatcccgg agggttctcc ggatccctat gcctttgtga tgaaggcggt gtccagcctg
      601 gataagatgt gtccaacgct tgatctgccc tacgcgacgc cgatcaacct ggtggtgaat
      661 gccagcaaca tgcccattga ccaaaacggc gtggtgatcg accgggcgaa cccgttggga
      721 atcccccgct acctcgatct ggatggcgac ggcgatttct tcgacgattc cttcctaagc
      781 gacacgcggt tacggccgtt gccgcacacg gacgccacga agcaattgaa ccactactcc
      841 atcgtggttc cgtccacggt ggtgggtcct atcgccgtga cgatggccag ttacttccag
      901 gcctttgagg gcattgtggc caagaagctc ctgggtaatc tggccaacac ggatgatgcg
      961 gacgccaacg aagggttccc gggcgggacg cctacgttgg agccctgcgt gctcaacggg
     1021 ccctgtgacc gggtcggcaa agccggtgcc accggagagg cgcagatccg tgaggcattg
     1081 aaatacgatc cggtggtggt cgaaggcgcg ccgccgactc cgatggaggt gaagaacgcg
     1141 gtgattcagg tgtccggaac caccgacaac gtcgccccga ggttgatcat caacaactcg
     1201 aagaataatc ccaacaccac ggcggccatt ccggcgaacc ggcactggtc accctctcca
     1261 tacggggggg cgaccggcaa ctatgccgcg gaagggatgg gtgaaatgaa cgtgagctgg
     1321 cggcgcgtgg tgaaggtcac ctttacggag ccggtgcggg aatgcgtcgg atccacctgc
     1381 ggtccgctga ccgcgaagac cttctatctc acggatgatg cggggaatat ggtgacggcg
     1441 ctggttgacc agatcgacga cacgacctgg gcgctgtttc cctacctcga cgatatgttg
     1501 aagccgacgt tcctggacgg aaaaacgtac gtgatcaacg tcgctccgtc cgtgagtacc
     1561 ggaggcgcca cctataagat cgtggacttc gccggcaacc aattgcaggc cggaccggct
     1621 tccgcaggga aatatacctt cgggttttcg gcgatggccc gtccatagct gtccgaagga
     1681 tacgattgtt tcgttaaaaa tcgtgtttcg tgaagaatca ggacgacggg ggtcgccatt
     1741 cggcgacccc cggtcgtttg tcctgaggtg tgcggaacgg ctataaggag gcgcgatgcg
     1801 tcaattcatc tctgggatgc ttcttggggt tgttctcttc tttttcttat ccttcttggg
     1861 acgttcaacc gcgggggctg tggagcaaca caccatggcc tacgtagcca acgccggcaa
     1921 caaccacgtc caggtcatcg acgtggattc tggccggaca ttgaccaagc tctataccgg
     1981 cgccacgccg tggaggcttc ggctcgcccc ggatgccaaa accctctggg tccagcattg
     2041 gtacgcccaa accacggccg tcgtggacct gaagactcag agcgtctccg cgattcttcc
     2101 cgagcgcggg cccgggatat tcaacgccaa aggtaatctc ttctggagct tttcctggcc
     2161 ggagagcact cttgaggcct atgacacgaa gcgctttaag cccgttcgga gcagagagac
     2221 aaacgccaag gttccctacg acttggccct tgcctggaaa gacagcagtc tgttcgtcgg
     2281 cgagtacgat cccgtctcga aaggcaatcg ccgtgtgtac gattttctcc gtgcctactc
     2341 catgaaggac gacaaggctc ccggcgtcag cctctcgctc ggcgacagtc cggcccggat
     2401 gcgtctggat cccaccggcc gttatctcgt cgtcgccaac ttggacggcg aggagctgtc
     2461 ccttgtcaat caaaataacg ctaaggccaa gatcaccctg gttccttccc cccaagacat
     2521 cttgttcggc ccgcatggtc ttatggtggt cctcgcctgg aagcccgaac ggcgtgcaag
     2581 cgagatcgtg actctgcagg tggattggtc gaagctgccc tggcccgagg tcaccccgtc
     2641 tgccagcaaa gaaatccagg gggggctcgt ggctggccag atggatccct ccggaaagcg
     2701 cttttatgcc ctcgatcgtc cgggccggcg cttggttgtt tttgatcttc ccgcgctgaa
     2761 cgaaatcaag tcgattccgg tgggagatgt tccctatgac ctcgtcgtca ctcaggtgaa
     2821 ccggaaacaa agggaccgct ggacggaaga gcggctgagc cagacccggc tcaagcggat
     2881 catcgagaac atgaaggctc agggcaaggc ctaccaggat ctgcagttcg tggaagagat
     2941 gcgtcttccc cagctcccac cggaacagga ggtggaatcc ggcgcgccgg aggagaaaac
     3001 agacaagact gccgagccga ccacgaccaa aatacaggtc cgcttccaag cccccgacag
     3061 cctgagaatc gaatatgaag acggcggcat ccgactggcc cagggtggga aagccgtcac
     3121 gatcacgccc gaagggcgtt tcttcgactc tccccgccaa gaccttctat atgttctcta
     3181 cgcactccat tctctcccgg tggaagacgt cattcggcat ctggccggcg acgtgccggg
     3241 cagcccattt ctcaagaacg gcattgcggt cgacgtcttg ggccaggtga aggagaatgg
     3301 ccatgtgtac gacgtgatcg ggacgcaaca gtctggggcg cgggtttccc aactctgggt
     3361 ggatgccaat cgctcgactc cggtgagttt cgtcgagcaa tttcccgtga tggaggccca
     3421 gtctccccat cagaaggccg gcacgctgtc ggggattgcc gaaaccaaat tgcagtatga
     3481 aaaacggcag gacggctatc aggttccggt ccggatggtt cgtgtggtgg atggcctgga
     3541 aaccggacgg gtgaccctgt cggacgttcg ggtgaatccc gggttgcaat ccgcgcaatt
     3601 cgatctggcc cggctgggag tcgtggcggt cacggcctca gagccgcatc acgcgaagac
     3661 cgtcgcgtcc aaacacgagc cgggccgcgt cgttccggga atgggcaacg cgcatatcga
     3721 tcatccggcc gctccccatg acccctataa cagcaatccc cccacgtcgg gaactcatac
     3781 tcggtatctg accgattggg gtattcataa gattcccatc ccgccggaga cgcaggtgca
     3841 caatctggag gacgggggcg tcctgctcca gtacaactgc ccgcaaggat gcgtggaatg
     3901 ggtgacccga ctggagcaat tggccatgaa gaacgacaaa gtggtcgtcg cgccctatcc
     3961 cctcatgaag gccaaattgg ccttaaccgc ttgggagcgt atcgagatat tggaccagtt
     4021 cgacgaagga cgcgtgacgg ccttcatcaa cgcgtatgcg gggaaggacc accacccgga
     4081 gggtggcgag ggggctgttg ccgacccgct gggcaaggcg ggcgaatgag gcgcgccggc
     4141 cctctgcttg cctgctgaga tgtgctcggt tccttccatc atgtggccga aatcgtcggg
     4201 tatccgcacc ccacgacctt gacaactgcc gtggcccatc ctccgggacg atttcctctg
     4261 tgaggaagaa ggagtcgaac gagaacccga cctgctgatc ggcagctatc gaggaaatgt
     4321 ggagcggagt aaagagccgg ggcaaaagcc ctccctccga gcct
//
LOCUS       DBNGTT010000453         2173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1129322, whole genome shotgun sequence.
ACCESSION   DBNGTT010000453 DBNGTT010000000
VERSION     DBNGTT010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1129322"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /gene="purB"
                     /locus_tag="AC16BC_10260"
     CDS             complement(<1..1258)
                     /gene="purB"
                     /locus_tag="AC16BC_10260"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006323587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3242042.1"
                     /translation="MIDRYSRPAMRALWSPSHKYALWLKVELAACEAMAAEGRIPEAA
                     VAVIRSRAKVDVARIEELERITQHDVLAFVNAVAETVGPEGRFLHEGLTSSDVLDTAL
                     ALTLREATAILREDLARLHRVLKDLAHRHRGTVMIGRSHGVHGEPITFGLKMAIWYEE
                     VGRHRQRLDRAEEEIRVGKLSGSMGTFAHLPPSIETRVCERLGLLPDPVSNQIVQRDR
                     HAAYMAALGLIAASLEKFAVEIRHLQRTEVLEAEEYFAAGQKGSSSMPHKRNPIGSEN
                     ISGLARVIKANVQAALDNVALWHERDISHSSVERLILPDSTALLDYMLDRFTTLLERL
                     QVYPERMRKNLDQTGGLVYSQRVMLALVDSGMARDDAYALVQGLAMRAWKGDGRFTDL
                     VRADTAIAGRLGEDALTACFDPGYFVR"
     gene            complement(1271..1771)
                     /locus_tag="AC16BC_10265"
     CDS             complement(1271..1771)
                     /locus_tag="AC16BC_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3242043.1"
                     /translation="MRVTVIAEPPARVCADVLVTGFFEDVRPPQGMAGQVDWLANGGL
                     SRLIVDQRVSGRLAETALLSVPTFSTPRVLFVGLGRVSNYTYLVLHQVAEALRPVLGA
                     LQVKCAAIELMETQACGLDPVIAARTFLKAWYQGRSDDGIELAFVAPKGSQPQQIEQR
                     LREMGV"
     gene            complement(1749..>2173)
                     /gene="nusB"
                     /locus_tag="AC16BC_10270"
     CDS             complement(1749..>2173)
                     /gene="nusB"
                     /locus_tag="AC16BC_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3242044.1"
                     /translation="DLACAESAGRAREPRNLAEFWAQRRASAEVRSFAESLIHGVDQH
                     QGEIDAIVSRHATHWSLDRMAAVDRNVLRLAVYELLYAREVPERVVLNEAIEIAKKYG
                     ADESGAFVNGILDHIIKHDPAVTAQRDAERTHAGDGHR"
BASE COUNT          389 a          756 c          643 g          385 t
ORIGIN      
        1 gccgcacgaa atacccggga tcaaaacacg cggtcagggc atcctcgccc agtcgccccg
       61 cgatcgcggt atccgcgcgc acgagatccg tgaagcggcc atcccccttc cacgcacgca
      121 tcgccaaccc ctggacaagg gcgtacgcgt cgtcccgcgc catcccgctg tccacaagag
      181 ccagcatgac gcgttgcgaa tacaccaagc cgccggtttg atcgaggttc tttcgcatcc
      241 gctccgggta gacttgcagc cgttcgagca gcgtagtgaa ccgatcgagc atgtaatcga
      301 gcagcgcagt ggaatcggga agaatgagcc gttccaccga ggaatgggaa atatcgcgct
      361 catgccacaa cgccacgttg tcgagggccg cctgcacgtt agccttgatc acgcgcgcca
      421 atccggaaat gttttcggac ccgatcggat tgcgcttgtg cggcatgctg gacgagcctt
      481 tctgtcccgc cgcaaagtat tcctcggctt ccaacacctc ggtccgttgg aggtgccgga
      541 tctccaccgc gaacttctcc agactggcgg cgatcaaccc caacgccgcc atgtacgcgg
      601 cgtgccggtc gcgctggacg atttggttgg acaccggatc ggggaggagg ccaagacgct
      661 cgcacacgcg tgtttcgatc gagggcggca ggtgggcgaa cgtccccatg ctccccgaaa
      721 gtttcccgac gcggatttct tcttcggccc ggtccaggcg ctggcgatgg cggccgactt
      781 cctcatacca gatcgccatc ttgagtccga atgtgatggg ttcgccgtgc accccgtgcg
      841 agcgcccgat catcacggtc ccgcggtggc gatgggcgag gtctttgagc acccggtgga
      901 ggcgggccaa gtcttcgcgc aggattgcgg tcgcctccct cagagttagc gccaacgccg
      961 tatccaggac gtcggaggac gtcagcccct cgtgcaggaa ccggccttcc ggaccgaccg
     1021 tttcggccac ggcgttcacg aaggcgagca cgtcgtgttg cgtgatgcgc tccaactctt
     1081 cgatccgcgc cacgtcgacc ttggcccgtg accggatgac cgcaaccgcg gcttcgggaa
     1141 tccggccttc ggccgccatc gcctcacagg cggcgagttc caccttcaac cacagcgcgt
     1201 atttatgcga gggagaccac agagccctca tcgccggccg gctatagcgg tcgatcaccg
     1261 atgctcctcc ttacacgccc atctctcgca gccgctgttc gatctgctgc ggttgggatc
     1321 ctttcggcgc cacgaacgcg agttcgatgc cgtcatccga ccgcccctgg taccaggcct
     1381 tcaaaaacgt ccgcgccgcg atcacgggat caaggccgca cgcttgcgtc tccattaatt
     1441 cgatcgccgc gcatttgacc tgcagcgcgc ccaacaccgg tcggagcgct tccgccacct
     1501 gatgcaggac gagataggtg tagttcgaca cccgtcccaa ccccacaaac aacacccgcg
     1561 gggtcgaaaa cgtcgggacg gacaagagcg cggtctccgc caggcgtccg ctcacgcgct
     1621 gatcaacgat cagtcgggac aatccgccgt tcgccagcca gtcgacctgg cccgccatgc
     1681 cttgcggcgg ccgtacgtcc tcaaaaaacc ccgtgaccag cacgtccgcg cacactcgcg
     1741 cggggggctc agcgatgacc gtcacccgca tgggtccgct ccgcgtcccg ctgagccgtg
     1801 accgcggggt cgtgtttgat gatatggtcc agaatgccgt tgacgaaggc cccggactcg
     1861 tccgcaccgt actttttggc gatttcgatc gcttcgttca acaccacccg ttccgggact
     1921 tcacgggcgt ataacagctc gtacacggcc agccgaagca cgtttcgatc aaccgccgcc
     1981 atccggtcaa gcgaccaatg cgtggcgtgg cgggacacaa tggcgtcgat ctccccctgg
     2041 tgctggtcca ccccgtggat gagcgactcc gcgaacgatc ggacttccgc ggacgcgcgg
     2101 cgttgcgccc agaattcggc caggtttctc ggttcgcgag cccggcccgc agactcggcg
     2161 caggccagat ccg
//
LOCUS       DBNGTT010000454         2560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1768311, whole genome shotgun sequence.
ACCESSION   DBNGTT010000454 DBNGTT010000000
VERSION     DBNGTT010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1768311"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="yidC"
                     /locus_tag="AC16BC_10275"
     CDS             complement(<1..902)
                     /gene="yidC"
                     /locus_tag="AC16BC_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR3242045.1"
                     /translation="MERNAVLFLILSISIIIGYSVLFPPAPPPKPAGNEAAPVSPPAE
                     PATGAVVADAGGSSQATPSAAAPAVALPGEERSVVVETDLYRATLSSRGAVITSWRLN
                     RYKDGPTKDTLIDLYTPPPQGGLSPLAVVLDAGDEATRAAFRSGAYAVDGGDLRLSSD
                     HPSGTLKFETRGAGGETLVKRLTFHHDSYIVDVEVSTWGVPGAYRVVLGENFGIRQWG
                     DASFVGYIGPTSLIDGKIIQDKPSKLEQPAVHDGRVSWTALQDKYFLTALIPTSPSGT
                     VVAERFGDKRVTIGLRERAPADGKP"
     gene            complement(966..1220)
                     /gene="yidD"
                     /locus_tag="AC16BC_10280"
     CDS             complement(966..1220)
                     /gene="yidD"
                     /locus_tag="AC16BC_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR3242046.1"
                     /translation="MNATPSVSPGNRRGVAAAVCVALVQGYRLAISPWLRPRCRFLPT
                     CSAYSVEAFEHYGAAKGLWLTVRRLLRCHPLHAGGYDPVP"
     gene            complement(1217..1552)
                     /gene="rnpA"
                     /locus_tag="AC16BC_10285"
     CDS             complement(1217..1552)
                     /gene="rnpA"
                     /locus_tag="AC16BC_10285"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR3242047.1"
                     /translation="MSRITRGPDIRRIFERGKRLSRGPLTLIGLRRTDTPEARRAGVI
                     VGRKFGGAVARNRIKRRVRELLRTHPESVPPGWDWLLLPRSPVQSWSFITLNQRITAL
                     FVEWTRTQA"
     gene            complement(1567..1704)
                     /gene="rpmH"
                     /locus_tag="AC16BC_10290"
     CDS             complement(1567..1704)
                     /gene="rpmH"
                     /locus_tag="AC16BC_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004139783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR3242048.1"
                     /translation="MSVTVKQPSNLRRKRTHGFRKRMSTTDGRRVLKRRRAKGRKRLT
                     V"
     gene            1867..2472
                     /gene="pyrE"
                     /locus_tag="AC16BC_10295"
     CDS             1867..2472
                     /gene="pyrE"
                     /locus_tag="AC16BC_10295"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3242049.1"
                     /translation="MITVTSATQSDHDMLLRLLFERAFRYSPEPTFRLASGALSRYYL
                     NGKLVTLDPKGATLIGRLVFERLRGLGVHAVGGLTLGADPIAIATAIASEAAGEPIAA
                     FVVRKEPKGHGTAQQIEGPLPPGVPVAVVDDVVTTGGSTEKALDVLDQLGHPVLKVVA
                     LVDRNEGGREAITRRGVPFESLFQITDFLALWNAEPSRAVL"
BASE COUNT          466 a          851 c          815 g          428 t
ORIGIN      
        1 ggcttcccgt cggcgggcgc gcgctcacgc agcccgatcg tcacgcgctt gtcgccgaat
       61 cgctccgcga ctaccgtccc gctgggcgac gtcgggatca gcgcggttag aaaatacttg
      121 tcctgtaacg cggtccaact cacacgacca tcgtggaccg cgggctgctc gagcttggac
      181 ggcttgtctt gtatgatctt gccgtcgatc aggctggtgg gtccgatgta gcccacgaat
      241 gaggcatcgc cccactgcct gatgccgaag ttctcgccca acaccacgcg gtaggcgccg
      301 ggcacgcccc acgtggacac ctcgacatcc acgatatagc tgtcgtgatg aaaggtgagc
      361 cgtttgacca gggtttcgcc gccggctccg cgcgtttcaa acttcagggt tccgctcgga
      421 tgatccgagg aaagccggag gtcgccgccg tccaccgcgt acgcgccgga tcgaaacgct
      481 gcacgggtgg cctcgtctcc tgcatccaac accaccgcca atggtgacag cccgccctga
      541 ggcggcgggg tgtacaggtc gatcagcgtg tccttggtcg ggccgtcctt gtaccggttc
      601 agccgccacg aggtgatcac cgccccacga ctcgacagcg tcgcccgata caggtcggtt
      661 tcaaccacca cgctccgctc ttcaccgggc aatgccactg ccggcgctgc cgccgatggc
      721 gttgcttgcg aggagccgcc cgcgtcggcc accacggccc cggtcgcagg ttcggccggc
      781 ggcgagaccg gagccgcctc gttcccggcc ggcttcggag gcggagccgg gggaaacagc
      841 acggagtagc cgatgatgat cgagatcgac agaatgagaa acaggacggc gttgcgttcc
      901 atgtcgtggc gactgggctc gggttagtcg gaacagcggg tgaacgtcaa cgtctcacca
      961 aggcgtcacg ggaccgggtc atacccgccc gcgtgcaagg ggtgacatcg cagcaggcga
     1021 cgaaccgtga gccacaggcc cttggcggcc ccgtagtgtt caaaggcctc caccgagtac
     1081 gcggaacacg tcggcagaaa ccggcatcgc ggccgcaacc acggcgagat cgccagtcga
     1141 tacccctgca ccagcgccac gcacaccgcg gccgccacgc cgcgtctgtt gccggggctc
     1201 accgacggcg tggcgttcat gcctgggtcc gcgtccactc gacaaacagc gcggtgatcc
     1261 gttgattcaa ggtgatgaat gaccatgact gcacgggaga acgggggagc aggagccaat
     1321 cccagccggg aggaacagac tcggggtggg tccgcagcag ttcgcgcacg cggcgcttga
     1381 ttcggttgcg ggcgaccgct ccaccgaact tgcgccccac gatcacaccg gcgcgacggg
     1441 cctccggcgt atccgttcgg cgcaggccga tcaacgtgag cggcccacgc gacaaccgct
     1501 tcccgcgctc gaagatcctt cgaatgtcgg gaccgcgggt aatgcgactc acccctgcgg
     1561 cgctcctcag accgtcaggc gttttctccc cttggcgcga cggcgcttga gcacgcgacg
     1621 cccgtcggtg gtggacatcc gcttgcgaaa tccgtgggtg cgcttgcggc gcaagttact
     1681 cggttgtttg acggtaacgg acatgggacc acactcctct gcttgttatt tccaaaaaaa
     1741 cgccctatta tagggaaccc ctgaaggggt gtcaaacctg ccggtcgaaa cgagctcggc
     1801 tgaaccagca ggccgcgacc aacggcgcgc atgaaggccg ccggttcaac gcgcaaggat
     1861 cagcgcatga tcaccgtgac ttcggccaca caaagcgatc acgatatgct cttacgcttg
     1921 ctcttcgagc gggcgttccg ctatagccct gaaccgacct ttcgtctggc ctcgggcgcg
     1981 ctcagtcggt actacctcaa cggaaaactt gtgacgctcg accccaaggg cgccacgctg
     2041 ataggccgcc tggtattcga gcgccttcgc gggttgggtg tacatgccgt gggcggtctg
     2101 accttgggcg ccgaccccat cgcgatcgct accgcgatcg ccagcgaggc cgccggagag
     2161 ccgatcgccg cgttcgtggt gcgaaaagaa cccaaaggcc atggaaccgc ccaacaaatc
     2221 gaagggccct tgcccccggg tgttcccgtg gccgtggtgg acgacgtggt gaccaccggc
     2281 ggctccacgg agaaggcctt agacgtcctt gaccagctcg gtcatccggt gctgaaggtc
     2341 gtcgcgctgg ttgaccgaaa cgagggcggc cgcgaggcca tcaccagacg gggcgtgccg
     2401 tttgaaagcc tgtttcagat caccgacttc ctcgccttgt ggaatgccga gccctcacga
     2461 gccgtcttat aaagggttgg gccgcatcag caggcccgta ggacggcgtg ggaagtttgg
     2521 cggccgcaga gacgccagtc ccaagcctca atcgaacagc
//
LOCUS       DBNGTT010000455         2892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3427857, whole genome shotgun sequence.
ACCESSION   DBNGTT010000455 DBNGTT010000000
VERSION     DBNGTT010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3427857"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /gene="mnmA"
                     /locus_tag="AC16BC_10300"
     CDS             complement(<1..1049)
                     /gene="mnmA"
                     /locus_tag="AC16BC_10300"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3242050.1"
                     /translation="MNKVTHRSKKVVVGLSGGVDSAVVASMLVAQGYRVEGITLRTWS
                     EPGEREKRWQDRSCCKIGIAQYVSQELCIPHRVIDAQERFRRAVIDDFCATYARGRTP
                     NPCVLCNERVKFALLVQAAEEAGADLLATGHYARVSHDATSNRYQLAKGVDPAKDQSY
                     FLYRLSQAQLGRALFPLGGFTKADVWIKAQAMGLPVDEIAESQEICFVTQKDYREFVA
                     THLPQTEQPGAMVGVGGEELGSHRGVAFYTVGQRRGLGVAAGERRYVTKLDAGHNLVV
                     LGAEDDLYSAACLVEDVHFGAMGAINAPIRVSAKYRYKTPEATATVSPADDGLLRVTF
                     DTPQRAITPGQSIVF"
     gene            complement(1396..1815)
                     /locus_tag="AC16BC_10305"
     CDS             complement(1396..1815)
                     /locus_tag="AC16BC_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR3242051.1"
                     /translation="MLTKKDGDKRNGTADEIIAFLGKGTRFKGVVTYDGTVRIDGHIE
                     GEVISTGTLIVGETAVIDAEVTVGSLICGGKITGNITATERVQLLNPASVTGSIKTPV
                     LMIEAGVRFNGQCEMKGDSRAEMIVDHEPLAIAANDR"
     gene            complement(1848..2675)
                     /locus_tag="AC16BC_10310"
     CDS             complement(1848..2675)
                     /locus_tag="AC16BC_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3242052.1"
                     /translation="MGDAGRPADRQEERGIMIPVHLIARSPHQPRKRFSETSLEELAE
                     SLREQGILQPVVVRKIPNGYELIAGERRWRAAQRAGLAEIPAVVKEAAPAEAAVLALI
                     ENLQREDLNPVEAAQAFRRLMVQFGLTQDEVARRVHKDRSSVANTLRLLQLPEELQQA
                     VLDDRLSHGHAKVLLGLDPDIQLILGRRVIAEGLSVRRTEDLAGAVTRRTRRRVTPVE
                     TADLELRLSRAIGTRVTVRGTARAGQIRIQYASSQELDHLLEWLEAGPNAARSSTGS"
     gene            complement(2750..>2892)
                     /locus_tag="AC16BC_10315"
     CDS             complement(2750..>2892)
                     /locus_tag="AC16BC_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3242053.1"
                     /translation="FDAIIPRNVTLAEAPSHGKPVLLYQIASRGAQLYMQLAKEVLKR
                     TA"
BASE COUNT          469 a          997 c          887 g          539 t
ORIGIN      
        1 tagaacacga tggactggcc cggggtgatg gcgcgctgcg gggtgtcgaa cgtcacgcgc
       61 agcaggccat cgtcagccgg gctcaccgtg gccgttgcct ccggggtctt gtaccggtac
      121 ttggcggaca cgcgaatcgg agcgttgatc gcgcccattg cgccgaagtg cacgtcctcg
      181 accaggcagg cggcgctgta caagtcatcc tcggcgccga gcactaccag gttgtggccg
      241 gcgtccagct tggtcacgta acgtcgctcg cccgcggcca cgcccaggcc gcggcgctgg
      301 cccacggtgt agaacgccac tccgcggtgg gatcccaatt cctcgccgcc cacgcccacc
      361 atggcgccgg gctgctcggt ctggggaagg tgggtcgcga cgaattcgcg gtagtccttc
      421 tgcgtgacga agcagatctc ctggctctcc gcgatctcgt ccacgggcag gcccatggcc
      481 tgggccttga tccagacgtc ggccttggtg aacccgccga gcggaaacag cgcgcggcca
      541 agctgggcct gcgacaagcg gtacagaaag tagctctggt ccttggctgg atccacgccc
      601 ttggcaagct ggtagcgatt gcttgttgcg tcgtggctca cgcgcgcgta atgcccggtg
      661 gcgagcaggt ccgcgcccgc ctcctccgcg gcctggacca gcaacgcgaa cttcacccgc
      721 tcgttgcaca acacgcacgg attgggcgtg cggcctcggg cgtacgtggc gcaaaaatca
      781 tcgatcaccg cgcggcgaaa tcgttcctgc gcgtcgatca cgcgatgcgg aatgcacaac
      841 tcctgtgaca cgtactgcgc aatgccgatc ttgcagcacg agcgatcctg ccagcgcttc
      901 tcgcgctcgc ccggctcgga ccacgtgcgc aatgtgatgc cttcgacgcg atagccttgg
      961 gccacgagca tggacgccac caccgcggaa tccaccccgc cgctcaaacc cacgacgact
     1021 ttcttcgacc gatgagtgac cttgttcata accatgcttg ctttgagggc cggcggctta
     1081 gccgcacgct ttaaatcccc ccttcccccc ctttgcttcg ccatgtgtga ggtcgccttc
     1141 ggcccatacg cacattttca aaggggggaa ccttattaac gtaaatatgg gcggatatcc
     1201 tgtcttgttt gaaccccaaa atgcaagggc ggcctcagtg ggccgcccta tactttgctt
     1261 tcggccgcca agaaggtggc ggatgcaagg cggaaacgaa ggttcgcccc ggagcgtact
     1321 gtatcgtacg tgaggagcga acctgagttg acaacgcggc cagccgcccc ttatcggcgg
     1381 ctggccgcga acgacctacc ggtcgttcgc ggcaatggcg agcggctcgt gatcgacgat
     1441 catctccgcc cgcgagtcgc ccttcatctc gcactgcccg ttaaaccgca cgcccgcttc
     1501 aatcatcagc accggggtct tgatcgaacc cgtgaccgac gcggggttga gcaactggac
     1561 cctctcggtg gcggtgatgt tgcccgtgat cttgccgccg cagatgagcg agccgaccgt
     1621 cacctcggcg tcgatgaccg cggtttcgcc cacgatcagc gtgccggtgg agatgacttc
     1681 gccctcgatg tggccgtcga tgcggaccgt gccgtcgtag gtgaccacgc ccttgaagcg
     1741 cgtgcccttg cccagaaagg cgatgatctc gtccgccgtg ccgttgcgct tgtctccgtc
     1801 tttcttcgtc aacatcgcgt gatctcctta cgtgtgtgtc ggggtcctca tgacccggtc
     1861 gatgagcggg ccgcgttggg tccggcctcc agccattcta gcagatgatc caattcctga
     1921 ctcgacgcgt actggatgcg aatctgtccg gcgcgcgccg tgcccctgac cgtgacgcgc
     1981 gtgccgatcg cgcgcgagag ccgcaactcc agatccgccg tttccaccgg cgtcacgcga
     2041 cggcgggtcc tgcgcgtgac cgcgccggcc agatcctcgg tccgccgcac cgacaggcct
     2101 tccgcgatca cccggcgacc caagatcagt tggatgtccg gatccagccc cagcagcacc
     2161 ttggcgtgcc cgtgactcaa ccgatcgtcg agcacggcct gctgcagctc ctcgggcaac
     2221 tgcaacaagc gcagggtgtt ggccacgctc gagcggtctt tatgcacgcg ccgagccact
     2281 tcgtcctggg tcaacccgaa ttgcaccatc aaccgccgga acgcctgcgc ggcctccacc
     2341 gggttgagat cctcgcgctg caggttctcg atgagcgcca gcaccgcggc ctcggccggc
     2401 gccgcttctt tgaccaccgc cggaatttcg gccaggcccg cccgttgcgc ggcgcgccac
     2461 cggcgttcgc ccgcgatcaa ctcataccca tttggtattt ttcgaaccac caccggctgg
     2521 aggatccctt gctcgcgcaa cgactcggcc aattcttcga gcgaggtctc cgagaagcgc
     2581 ttgcgcggct ggtgcgggct ccgggcaatc aggtgcaccg ggatcatgat cccgcgctcc
     2641 tcctggcgat ccgcgggacg ccccgcatca cccaacaacg cctccaggcc gcggccgagc
     2701 gccttgcgtt caccgcctgg gcgcgttttt gccgttgatg ggggggaatc tacgccgtcc
     2761 gtttgagcac ctccttcgca agctgcatgt agagttgcgc gccccgcgag gcgatctggt
     2821 agagcaacac gggtttgccg tgactcgggg cctcggccag ggtcacgttg cgcggaataa
     2881 tcgcgtcgaa ca
//
LOCUS       DBNGTT010000456         4796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2024262, whole genome shotgun sequence.
ACCESSION   DBNGTT010000456 DBNGTT010000000
VERSION     DBNGTT010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2024262"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1716)
                     /locus_tag="AC16BC_10320"
     CDS             complement(7..1716)
                     /locus_tag="AC16BC_10320"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HIR3242054.1"
                     /translation="MIHPGGHDELQIRVGFLSETGRREANEDFVGSHVGDARQRAVKG
                     CVVAVADGVGGARGGRQAAEVTVRGFLDGYLGLPDTLGVERAAGRALDAMNRWVFAQG
                     RQDHSLEHMATTFSALVLRGRQAHTIHVGDSRIYRLRDNRLTRLTTDHTHGHPDLRHV
                     LYRAVGIEAILRADYAADGIKLHDRFVLCSDGVHAVLSDRAIQGVLSERTSPQESAER
                     LVRAALDAGSQDNATALVVDVVGLPAADEAEFEGAIDVLPIGDLPKAGELVDGFLLGN
                     VLSSGRYSRLFRAKDTLDGRDVALKFPHPRVAGEASYRRAFVREAWIASRVTSPWIGD
                     VIELPPGRRTRLYSVMPYYEGETLESRLARQPAVSLEEGVRIGIALAKAVYGLNRLRI
                     IHRDVKPDNVLVQPDGGLKLLDLGVARLPGIQDAPSDEIPGTPSFMAPELFAGEAGDD
                     QSDVYALGVTVYRLFSAGAYPYGEVEAFSRPRFTKRTPLTHYRPDLPAWLDAVLAKAT
                     AVDKAQRFGDSMEMAFELENGLAQGGQRGSDRVPLYHRNPVRFWQVVSALLALALLAL
                     LTT"
     gene            complement(1910..3193)
                     /locus_tag="AC16BC_10325"
     CDS             complement(1910..3193)
                     /locus_tag="AC16BC_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3242055.1"
                     /translation="MWNLGEFRRAGHWPTLLSAFLYFDVSFMVWVVLGPLALYISQDL
                     ALSVEEKFGVVAIPILFGAALRIPLGMLADQIGPKRAGQAGQLAVMAGLAYAWVIGLN
                     SKLDVELLGVILGVAGASFAVALPQASRWYPPRYQGMVLGIAAAGNMGVVLDSLFVPW
                     LAEQFGWRAVFGILLIPAVAVFLLYSVVAKDAPDDRLPVTRANYVSVLKDPDTWWFMF
                     FYSITFGGFVGLSSALPLYFTQWYHASGIAAGLMVAIVVFAGSLFRPVGGWLADRMGG
                     IRTLQTLFAVVSLMYLTVAFMPEGPAPATESVALAKVTGWNLADLPGGAWTSVGIFFV
                     GAVALGMGNGSVFQLVPLRFRREMGVMTGLVGAAGGIGGFVLARALGWSQGLTGNFAM
                     GFLTFSGLALVGLVGLVVVKARWRTTWGAVSGARV"
     gene            complement(3339..3677)
                     /locus_tag="AC16BC_10330"
     CDS             complement(3339..3677)
                     /locus_tag="AC16BC_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3242056.1"
                     /translation="MKKIEAIIKPHKLDDVKAALMDLGVHGMTVLEVKGFGRQKGHKE
                     VYRGAEYVVEFVPKTTIELVVSDDLAPRVVDAIVRAARTSSIGDGKIFVTSISDAIRI
                     RTGETGDNAL"
     gene            complement(3917..>4796)
                     /locus_tag="AC16BC_10335"
     CDS             complement(3917..>4796)
                     /locus_tag="AC16BC_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3242057.1"
                     /translation="TPALIVGAFAERMRFSAVLIFSSLWLLLVYAPICHWVWGGGWLG
                     KLGAVDFAGGAVIHISSGASALACALMLGARKGYGTANMAPHNLPMALLGTGLLWFGW
                     FGFNAGSGLGANSTAVVAFTATHIAAAAGAISWMAAEWAQRGKPTVLGVASGVIAGLA
                     TVTPGAGYVGPFSALLIGLVAGAACYFAVVAKARLGYDDSLDVVGIHGVGGVIGILAT
                     GLLASVGVKGLLFGNPGQLGVQALLAVVSAVFAFVGSIVILKIVDAVSGLRVSIEDEQ
                     KGLDLSQHEERAYSNE"
BASE COUNT          932 a         1721 c         1452 g          691 t
ORIGIN      
        1 tccgatctat gtggtcagca gggctaacag cgcgagcgcc agcaaggcgg agaccacctg
       61 ccagaaccgc accgggttgc ggtgatacag gggcacacgg tcagacccgc gctgaccgcc
      121 ctgggccaga ccgttttcca actcaaacgc catttccatc gagtctccga accgctgcgc
      181 cttgtccacg gccgtggctt tggccaacac cgcgtccagc cacgccggga gatcgggccg
      241 gtaatgggtc aagggggtcc gtttggtaaa gcgcgggcga gaaaacgcct ccacttctcc
      301 gtagggatac gcccccgcgc tgaacaggcg atacaccgtg acgcccaacg cgtacacgtc
      361 ggactggtcg tcgcccgcct cgccggcaaa cagctcgggc gccatgaagc tcggcgtgcc
      421 cggaatctcg tccgacggcg cgtcctgaat ccccggcaag cgcgccaccc ccaggtccaa
      481 gagtttaagc ccgccgtccg gctgcaccag cacgttgtcg ggcttgacgt ctcggtgaat
      541 gatccgcagg cgattcaatc cgtacacggc cttggccagc gcgatgccga tccgcacgcc
      601 ctcttccagc gagaccgcgg gctggcgcgc caggcgcgac tccaaggtct cgccctcgta
      661 gtacggcatc accgagtaca gccgggtcct gcggccaggc ggcagctcga tcacgtcgcc
      721 gatccacggg ctcgtcaccc gcgaggcgat ccacgcctcg cgcacgaacg cgcgccggta
      781 ggacgcctcg cccgcgaccc tggggtgcgg aaacttgagc gccacgtctc gcccgtcgag
      841 ggtgtctttg gcccggaaca ggcggctgta ccggccgctc gacagcacgt ttcccagcag
      901 gaacccgtcc accagctcgc cggccttcgg caggtcgccg atgggcaata cgtcgatcgc
      961 cccttcgaat tcggcctcat ccgcggcggg caatcccacg acgtccacca ccagcgccgt
     1021 ggcattgtcc tggctgcccg cgtcgagcgc cgcgcgcacc agccgctcgg cgctctcctg
     1081 cggagacgtt cgctccgaca ggaccccctg gatcgcgcga tccgacaaca ccgcgtgcac
     1141 gccgtcgctg cacagaacga accgatcgtg gagcttgatc ccgtccgcgg cgtaatccgc
     1201 gcgcaggatc gcttcgatgc ccacggcgcg atagagcacg tgacggaggt ccggatggcc
     1261 gtgggtgtga tcggtggtca accgggtcaa ccggttgtcc cgcaatcggt agatgcggct
     1321 gtccccgacg tggatcgtgt gcgcctgacg accgcgcagc accagggcgc tgaacgtggt
     1381 ggccatgtgt tccagcgagt ggtcctggcg cccctgcgca aacacccagc ggttcatggc
     1441 gtcgagcgcc cgacccgccg cgcgctccac gcccagcgta tccggcaggc ccagataccc
     1501 gtcgagaaac ccgcggaccg tgacctcggc ggcctggcgc ccgccgcgcg cgccgccgac
     1561 cccgtccgcc accgcgacca cgcacccctt cacggcgcgc tgacgcgcgt caccaacgtg
     1621 ggacccgacg aaatcctcgt tggcctcgcg tcggccggtc tcgctcagga atccgacgcg
     1681 gatttgcagt tcgtcatgcc ccccgggatg aatcatcggc ccgctattct acacccgcgc
     1741 gccgatgaaa cgagcaggca ctcctcgcgt agagctgaga gagcctgttc aggatgggcc
     1801 agatgccagg cggaggaagg gggttgcgcc cggagcgtac cggttcgtac gtgaggacgc
     1861 aacgccttcc aacaacgccg cagatggccc gtcatggaca ggctcccacc tacacccgcg
     1921 cgcctgacac agccccccag gtggttctcc accgcgcctt gaccaccacc aatccgacca
     1981 accccaccaa ggcgagcccg gaaaacgtca ggaaccccat ggcgaaattt ccggtcaacc
     2041 cttgcgacca tccgagggct cgggccaaga cgaatccccc gatcccaccg gccgcgccga
     2101 ccagtccggt catcacgccc atctcgcggc gaaagcgcag cggcaccaac tgaaacaccg
     2161 atccgttccc catgcccaag gccacggccc ctacgaagaa aattcccacc gaggtccagg
     2221 cgcccccggg cagatcggcc aggttccacc ccgtcacctt ggcgagcgcg acggattccg
     2281 tagcgggcgc cggcccctcg ggcatgaagg ccacggtcag gtacatgagc gacaccacgg
     2341 caaagagcgt ctgcaaggtc cggatgccgc ccatgcggtc ggccaaccac ccgcccaccg
     2401 gcctgaacag agaccccgcg aacacgacga tcgcgaccat caggcccgcg gcaatgccgg
     2461 aggcgtgata ccactgggta aaatagaggg ggagcgcact gctcagtccc acgaacccgc
     2521 cgaaggtgat ggaataaaaa aacatgaacc accacgtgtc ggggtccttg agcaccgaca
     2581 cgtagttcgc tcgcgtcacc ggcagccgat catccggcgc gtccttggcc accaccgagt
     2641 acaggaggaa caccgcgacc gcggggatca gcaggatgcc gaacaccgcc cgccagccga
     2701 attgttcggc caaccacggc acgaacagcg aatccaacac cacccccatg ttgccggcgg
     2761 cggcaatgcc cagaaccatg ccttggtaac gcggcgggta ccagcggctc gcctgaggaa
     2821 gcgccacggc aaagctcgcg ccggcgaccc cgaggatgac tccgagcaac tccacatcca
     2881 acttactgtt caaaccgatc acccaggcgt aggccagccc cgccatgacc gcgagttggc
     2941 cggcttgtcc cgcgcgtttg ggaccgatct ggtcggccag catcccgagg gggatacgaa
     3001 gcgcggcgcc gaacaagatc gggatcgcca cgacgccgaa tttctcttca acgctgagcg
     3061 cgaggtcctg agaaatgtac agcgccaggg gaccgagcac cacccagacc atgaaactga
     3121 cgtcgaaata taaaaatgcc gacagcaagg tcggccaatg ccccgcccga cgaaactccc
     3181 cgaggttcca cacggatcgc ctccagccag tccgagagag caaagcaatc acgatgccag
     3241 agtcggtggc acccgtgaga gggactcggg aggtgccgga caaaatcgtc gaacggaaat
     3301 gtcgaagctg cgacaatttc gtcgcagtgc gggcgtcgct acaacgcgtt gtcgccggtc
     3361 tcgccggtcc ggatgcggat ggcgtcgctg atcgaggtca cgaagatctt cccgtccccg
     3421 atgctggagg ttctcgcggc gcggacaatg gcgtccacca cgcgcggcgc aagatcgtcc
     3481 gacaccacga gttcgatcgt cgtcttcggg acgaactcca ccacgtactc ggcgccgcga
     3541 tagacttctt tgtggccttt ctgtcgcccg aagcctttca cttcgagaac ggtcatgccg
     3601 tgcacaccca gatccatgag cgccgccttg acgtcgtcga gcttatgggg cttgatgatg
     3661 gcttcgatct ttttcatgca cacgccctca tcgatgatcg actcgctcgc tagaacgctt
     3721 gcccgtgcga gcctgttgag caatgcgcca catgcaaggc ggcgcgagaa ccgcaccgga
     3781 gcgtacgtaa tgggtacgtg aggagcggaa agcgcacggc gccgctgtgc tgcggcgctg
     3841 acaatggatc ggtcatttcg cttggcgaag caagcgcagc gccaacgcag cagatggcca
     3901 ttgatcgaca ggcgttctat tcgttggaat acgcccgttc ctcgtgctgg ctcagatcca
     3961 atcctttttg ttcgtcctcg atcgagacgc gcaggccgga caccgcgtcc acgatcttga
     4021 gaatcacgat cgagccgacg aacgcgaaca ccgcggacac gaccgccaac agcgcctgca
     4081 ccccgagttg gcccggattg ccgaacagca gccctttcac gcccaccgac gccagaagac
     4141 cggtcgccag gatgccgatc acgccgccga cgccgtggat gcccaccacg tccagcgagt
     4201 cgtcataccc gagccgggcc ttcgcgacca ccgcgaaata acacgccgcg cccgcgacca
     4261 acccgatcag cagcgcggag aacgggccga cgtatccggc gcccggcgtc acggtggcga
     4321 ggcccgcgat gacgccgctc gccacgccca gcaccgtggg tttgccgcgt tgcgcccact
     4381 cagccgccat ccaggagatc gcgccggcag ccgccgcaat gtgggtggcc gtgaacgcga
     4441 ccaccgcggt cgaattcgcg cccaagccgc tgcccgcgtt gaatccgaac cacccgaacc
     4501 agagcaagcc ggtgcccagc agcgccatgg gcaggttgtg cggggccatg ttggcggtcc
     4561 cgtatccctt tctggcgccc agcatgagcg cgcacgccag cgcgctggcg ccggaactga
     4621 tgtgaatgac ggccccgccc gcgaaatcca cggcgccgag tttcccgagc catccgccgc
     4681 cccacaccca gtgacagatc ggtgcgtaca ccagcagcag ccagagcgag ctgaagatga
     4741 gcaccgctga gaagcgcatg cgctcggcga aggccccgac gatgagcgct ggcgta
//
LOCUS       DBNGTT010000457         6516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1385901, whole genome shotgun sequence.
ACCESSION   DBNGTT010000457 DBNGTT010000000
VERSION     DBNGTT010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1385901"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="AC16BC_10340"
     CDS             <1..279
                     /locus_tag="AC16BC_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242058.1"
                     /translation="WITLVEPPVGPGRALKPVAIGESAGGGYDEEISRAIAVLKERPD
                     DAQARFDLGITYLLTGKFDLALEESRILNMTDARLSAKLQALTKLLAT"
     gene            complement(289..1125)
                     /locus_tag="AC16BC_10345"
     CDS             complement(289..1125)
                     /locus_tag="AC16BC_10345"
                     /inference="COORDINATES: protein motif:HMM:NF046841.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3242059.1"
                     /translation="MTANAAARTPEPWTHAIVIHFQDGRIDRGVLTSSLFSGTTLTYR
                     PNLVTGERRTVVRVPLLTEAKIDRLTVGRVTDVSAQGAFVETLAPYPIGTALTISLRI
                     DNELINAPARVVFSDPGVGMGLEFTRPSTAEWNRVDAALHRLVRGERTQSAGRRRDAN
                     RRTGRGAPAGPRRWDARHRDRRHVAQPETAAPIVVDLAKVKSVFFIESNDQPRNGKSA
                     ADPHDREVTVEFRDGEMIHGTMGELDSDAPGFFVALRLDERHTHTVYVVKSAVKSIQT
                     AF"
     gene            complement(1208..1867)
                     /locus_tag="AC16BC_10350"
     CDS             complement(1208..1867)
                     /locus_tag="AC16BC_10350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3242060.1"
                     /translation="MNLIGLDLDGTLEDSRSDMTAAVRRVRAALGLAPRPDDAIRPWV
                     NRGMDQLYRACFDDYLNGDAARSVVVRERYETDYLANVAHETRLYPGIANALAKIADR
                     GRLAVITNKPERISHRLLEVLGVGKHFAAIIGGDTCARGKPDPSVLAEAAKRCGFVPG
                     RGRAVMIGDSAADVQLGRAFGAATIWCAWGYAAQPGEPPDFVARGPEQLPDLVATALT
                     S"
     gene            complement(1864..2289)
                     /locus_tag="AC16BC_10355"
     CDS             complement(1864..2289)
                     /locus_tag="AC16BC_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3242061.1"
                     /translation="MSERSKTATFPNGVKLIKWGRTEEPTADSVADAMRGFGYAVYDL
                     QTVAPWFERSRHAHDEPEIRGAVSGAITFHFDEFPITIEAGDILLIPGGLAHEVIGHN
                     SRPFSAYKGSLSGERKVTEHGDAKGSIEHLAAMQGSRRS"
     gene            2499..3635
                     /locus_tag="AC16BC_10360"
     CDS             2499..3635
                     /locus_tag="AC16BC_10360"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3242062.1"
                     /translation="MADLFRFADELGPVKIIHVYEPSIQLKAILVVDNVAAGPSIGGV
                     RMAPDVGVEECLRLARAMTLKNAAAGLAHGGGKAVVFGDPKMAKPKKEALIRGLAKAL
                     RNEEDYIFGPDMGTDEHCMAWVHDEIGRAVGLPRELGGIPLDDVGATGWGVAQVADVA
                     LEFCDVNRKKGVRVVVQGFGAVGHHAARFLADKGAVLVAASDSAGAIHDPDGLDVNEL
                     ITLKEQGKSVSAAAKGKSIDRDAVIDMDCDIWIPAARPDVVNEDNVHRLRAKLVIEGA
                     NIPITLGAEKVLAEKGILCVPDFIANAGGVICAAMEYHGTGESTVFQVIEDKLRRNTR
                     LVLEASASQRILPRQAAMDLAVQRVKKAMSMRRFSLFSSAPGFA"
     gene            3655..4593
                     /locus_tag="AC16BC_10365"
     CDS             3655..4593
                     /locus_tag="AC16BC_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3242063.1"
                     /translation="MYRVRFHGRGGQGIKTASRILGAAFFQAGFEVQDAPRYGAERRG
                     APIFAYVRASRQPINERGVIQRPDLVIVADETLMPVPAAGVLAGVRPRTVLLVNSHES
                     PESFRRRLNITSPVLILPAPAEADRAELVFIGATCAGAAARLVGVIPRDALEEAIRRE
                     LADFGSGIVAKNLEQAIAAFRSMQEHAGCVTEGDDASAVDYETPDWIDLPFEAARVSA
                     PVIHAGLTSVEVKTGLWRTLRPVIDYERCHRCWSICSTLCPDSAINVTPDGWPEIDYD
                     HCKGCMVCVAVCPTHAIDAIPEHVAKAQEEQGKPSA"
     gene            4590..5837
                     /locus_tag="AC16BC_10370"
     CDS             4590..5837
                     /locus_tag="AC16BC_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HIR3242064.1"
                     /translation="MTRTLLTGNGAAAWGARLAKVDYVPAFPITPQTEIIESLSNWIE
                     RGDMRSRLVTLESEHSMITAAGSAAATGVRVFTATSSQGLLYGMEMLYNAAGWRAPFV
                     LVNVSRGLAAPLTLEPDHNDVLAARDSGCLQIHCATCQEVLDTTVLAFRLAEDARVRL
                     PVIVNLDGFYLSFTREPVVIPDAEAVDAFLPPFAPGNMAFRASMPTSQAVVVLGGSSY
                     SYFRYEMHLAALKALDVYDEIAAEFAKKFGRTHEAIETYRCEDAEIVFVMMGSFATKA
                     KAAVDGLRAAGQAVGLVRPRLLRPFPANHLQRVLAGKRGVAVIDQNLSLGKGGVLHAE
                     LASVLYGRPDAPTILASFIGGLGGRDITDEEFFEIARVTRQAVARNQTPPPRLLYNRD
                     ELMQIQKLQAIAHVEREELRKNP"
     gene            5834..>6516
                     /locus_tag="AC16BC_10375"
     CDS             5834..>6516
                     /locus_tag="AC16BC_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3242065.1"
                     /translation="MSDTETLFKRLQDLPSTHLLGGGTSMCAGCGSLQALHEIYDILG
                     AKTVFVNAAGCMTLLSTYPFTPFRGSWLYTAMASAPAGAQGVRDALDILLESNRIPPE
                     ENLDVVVLTGDGSAYGMGLSATSAAMERNLDFIYICYDNEGYANTGQQASGATPHAAK
                     TATSTGACGFSGYKKDLFAIWAAHKPAYIATVIGADPLDLARKIEKAKRMKGPRMIIA
                     LAPCPTGWE"
BASE COUNT         1133 a         2070 c         2166 g         1147 t
ORIGIN      
        1 tggatcacgc tggtcgagcc gccggtgggt ccgggtcgcg cgctcaaacc cgtcgccatc
       61 ggggaatccg ccgggggcgg gtacgacgag gagatctcgc gcgcgatcgc cgtcctcaag
      121 gaacgtcccg acgacgcgca ggcgcgcttc gacctcggga ttacctatct cctgacgggg
      181 aaattcgacc tcgcgttgga agagtcgcga attctgaaca tgacggacgc gcggttgtcg
      241 gccaaactgc aggcgctgac gaagctgctg gcgacctgat cgccagagtc agaacgcggt
      301 ctggatgctc ttgaccgcgg atttgaccac gtagacggtg tgggtgtgcc gctcgtcgag
      361 gcgcaacgcg acgaagaacc ccggcgcgtc cgaatcaagc tcccccatcg tgccgtgaat
      421 catctcgccg tcgcgaaatt cgacggtgac ctcccgatca tgcgggtcag cggctgattt
      481 cccgttgcgc ggttggtcgt tggattcgat aaaaaacacg gacttcactt tcgccagatc
      541 gaccacgatg ggcgcggcgg tttccggttg cgcaacgtgc cggcggtcgc ggtgacgagc
      601 gtcccaccgc cgcggcccgg cgggcgcgcc gcgtccggtc cgtcggttcg cgtcccgccg
      661 ccggcccgcc gactgcgtcc gttcaccgcg gacgaggcgg tgcagggccg cgtcgactcg
      721 gttccactcg gccgttgacg gtcgcgtgaa ttccaggccc atccccaccc cgggatcgga
      781 gaagaccacg cgcgccggcg cattgatcaa ctcgttgtcg atccgaagcg agatggtcag
      841 ggcggtgccg atcggatacg gcgcgagcgt ctccacgaac gcgccttgcg cgctcacgtc
      901 cgtgacgcgg cccacggtca ggcggtcgat cttcgcctcg gtgagcaacg gcacgcggac
      961 gacggtgcgc cgctcgccgg tgaccaggtt cggccgatag gtcagggtcg ttccgctgaa
     1021 cagggacgac gtcaagacgc cgcggtcgat gcggccgtct tggaagtgga tcacgatggc
     1081 gtgagtccag ggctcgggcg tgcgagcggc ggcgttcgcg gtcatggttt tcataatgat
     1141 ggggagagca cgagcgaaag tatagcaacc gcggagcgat cgacaagcgc gccgcggccc
     1201 cgcccgtcta cgacgtgagc gccgttgcga cgaggtcggg gagttgctcc ggtccgcggg
     1261 cgacaaaatc cggcggctcg cccggctgcg ccgcgtaacc ccacgcgcac cagatggtgg
     1321 cggctccgaa cgcgcgaccg agttgcacgt ccgcggcgct gtcgccgatc atgacggcgc
     1381 ggccgcgccc cggcacgaac ccgcagcgct tggcggcttc cgccaacacg ctcgggtcgg
     1441 gtttgcccct ggcgcaggtg tctcccccga tgatcgcggc gaagtgcttg cccacgccga
     1501 ggacttcgag caagcgatgg gagatgcgtt cgggtttatt ggtgatcacg gccagccgac
     1561 cgcggtccgc gatcttcgcc agggcgttcg cgatgcccgg atagagtctg gtctcgtgcg
     1621 ccacgttggc caaataatcc gtctcgtacc gctcacgcac cacgaccgac cgcgcggcat
     1681 ccccgttgag gtagtcgtca aaacacgcgc gatacaactg gtccatcccg cgattgaccc
     1741 acggccggat cgcgtcgtcg ggccgtggcg ccaggccgag cgctgcgcgc acgcgccgca
     1801 ccgcggcggt catgtcgctc cggctgtcct ccagggtgcc gtcaagatcc agcccgatca
     1861 ggttcacgaa cggcgcgatc cctgcatcgc ggccaagtgc tcgatgctgc ccttggcgtc
     1921 gccatgctcg gtgaccttgc gttccccgct cagggaccct ttatacgcgg agaacggccg
     1981 gctgttgtgg ccgatcacct cgtgcgccag gccgccgggg atcagcagga tgtcgccggc
     2041 ctcgatcgtg atggggaact cgtcgaagtg aaacgtgatc gcgcccgaca ccgcgccgcg
     2101 gatttccggc tcatcgtgcg cgtgacggct gcgctcgaac cacggcgcca cggtctgcag
     2161 atcgtacacc gcgtacccga acccccgcat ggcgtcggcc acggaatccg cggtgggctc
     2221 ctcggtccgt ccccacttga tcagtttgac cccgttggga aacgtcgctg tcttggatcg
     2281 ctcgctcatg gtcgggcctc ctctgcgggc acacgataac gcgttcacga ggccgatgtc
     2341 cattcctcca ttcgggggag cgacggccgc gcactgctcg atccagaacc agtgacaagg
     2401 ggcaacggcg ggcggtgaaa gccgtcaggg gcttgattct ctggcgcgct tcaagtccaa
     2461 tatgtatggc tcgtgctggg atcacccggg ggacaaccat ggcagatctg tttcgattcg
     2521 ctgacgagtt gggtccggtc aaaatcatcc acgtgtacga accctcgatc caactcaagg
     2581 cgatcctggt ggtcgacaac gtggccgcgg ggccttcgat cggcggggtg cggatggcgc
     2641 cggacgtggg cgtcgaagaa tgcctccgac tcgccagggc catgaccctg aagaacgcgg
     2701 ccgcgggcct tgctcacgga ggcggcaagg cggtggtgtt cggcgacccg aaaatggcca
     2761 aacccaaaaa agaggcgctc atccgcggcc tggccaaggc cctgcggaac gaggaggact
     2821 acatcttcgg ccccgacatg ggcacggacg aacactgtat ggcgtgggtg cacgatgaga
     2881 tcgggcgcgc ggtcggcctg ccacgtgagc tgggcggcat tccgctggat gacgtcggcg
     2941 ccacgggctg gggagtcgct caggtcgcgg acgtggcgct agagttctgc gacgtcaacc
     3001 ggaagaaggg cgttcgggtc gttgtgcagg gattcggcgc ggtgggccac catgcggcgc
     3061 ggtttctcgc ggacaaaggc gcggtcctgg tggcggcgtc cgattccgcc ggagccattc
     3121 acgatccgga cgggctcgac gtgaacgagt tgatcaccct caaggagcag gggaaaagtg
     3181 tgtcagccgc agcaaagggt aaatccattg atcgggatgc cgtcatcgac atggactgcg
     3241 atatctggat tcctgctgcg cggcctgacg tggtcaacga agacaatgtt caccgcttga
     3301 gggcaaagct ggtgatcgag ggcgccaaca tcccgatcac gctcggcgcg gaaaaggtcc
     3361 tggcggagaa aggcattctc tgcgtgcccg actttatcgc caatgctgga ggggtgatct
     3421 gcgcggccat ggagtaccac ggcaccggcg aatccacggt attccaggtc attgaggata
     3481 agctgcggcg caacacgcgg ctggtgctcg aggcgtccgc ctcacaacgc atcctgccgc
     3541 gacaggccgc aatggacttg gccgtgcaac gggtgaaaaa ggcgatgagc atgcggcggt
     3601 tctccctgtt ctcatcagct cctgggttcg cgtagtccac gaagtcgagg gagtatgtat
     3661 cgggtccgat ttcacggccg cggcggacaa ggcatcaaga ccgctagtcg catcctgggc
     3721 gcggcatttt ttcaggcggg ctttgaagtc caggacgcgc ctcgatacgg cgccgagcgt
     3781 cgcggtgcgc cgatcttcgc ctacgtgcgg gcctcgagac aacccatcaa cgagcgcggc
     3841 gtcatccagc ggccggatct ggtgattgta gcggacgaga ccctgatgcc agtgcctgcc
     3901 gcgggcgtgc tcgcgggtgt tcgcccccgg accgtgctgc tcgtcaacag tcacgaatcg
     3961 ccggaatcgt ttcgtcgccg gctgaacatc acgtcgccgg ttctgattct ccccgcaccc
     4021 gcagaggcgg accgcgcgga gctggtcttt atcggcgcca cgtgcgcggg cgcggcggcg
     4081 cggctggtcg gcgtcatccc tcgggacgcg ttggaagaag cgattcgccg ggagctggcg
     4141 gactttgggt cgggcatcgt ggccaagaat ctggagcagg cgattgccgc cttccgatcg
     4201 atgcaggaac atgcggggtg cgtgaccgaa ggcgatgacg cgtcggcagt cgactatgag
     4261 acgccggatt ggatcgatct gccgtttgag gcggcgcgcg tctccgcccc cgtgatccat
     4321 gcgggcttga ccagcgtgga agtgaaaacc gggttgtggc gcacattgcg tccggtgatc
     4381 gactacgagc gatgccatcg ctgctggtcg atctgcagca ccctctgtcc ggacagcgcc
     4441 atcaacgtga cgcccgacgg atggccggag atcgattacg accattgcaa aggctgcatg
     4501 gtgtgcgtgg cggtctgccc gacccacgcg atcgatgcga ttccagaaca cgtggccaag
     4561 gcgcaggaag aacaggggaa gccgtccgca tgacccgcac attgctgacc ggaaacggag
     4621 ctgctgcatg gggcgcgcgt ctggccaagg tggactatgt ccccgcgttt cccatcacgc
     4681 cccaaaccga gattatcgag tcactgtcca actggattga gcgcggcgac atgcgaagcc
     4741 gcctggtcac gttggaatcg gaacattcca tgatcacggc cgctgggagc gcggccgcaa
     4801 ccggtgtccg ggtcttcacc gccacctcca gccaaggatt gctgtacggg atggaaatgc
     4861 tttacaacgc ggcggggtgg cgcgcgccct tcgtcctggt caatgtctcc cgcgggctgg
     4921 ccgcgcccct gaccctggaa cccgaccaca acgatgtcct ggcggcgcgg gactccggtt
     4981 gtttgcagat ccattgcgcc acctgtcagg aagttctcga caccaccgta ttggcctttc
     5041 gcctggcgga agatgcgcgc gtgcggttgc ccgtgatcgt gaatctggac ggcttctatc
     5101 tgtcctttac ccgcgagccc gtggtcatcc ccgacgctga ggccgtggat gcgtttctcc
     5161 cgccattcgc tcccgggaac atggcgtttc gcgccagcat gcccaccagt caggccgtgg
     5221 tggtgctggg cgggtcatcg tattcttatt ttcggtacga aatgcacctg gccgccttga
     5281 aggcgctcga cgtgtacgac gagatcgccg cggagttcgc caagaagttc gggcggacgc
     5341 atgaggcaat cgagacgtat cggtgcgagg acgcggagat cgtcttcgtg atgatgggat
     5401 ccttcgcaac caaggccaag gccgcggtgg atggcttgcg cgcggcgggc caggcagtgg
     5461 gcctggtgcg gccgcgactg ttgcgtccct ttcccgcaaa tcacctgcaa cgggtgttgg
     5521 ccggcaaacg gggggtggcg gtcattgatc agaatctgtc cctgggcaag gggggcgtgc
     5581 tccatgccga gctggcctcc gtgctctatg gtcggccgga tgccccgacc atcctggcca
     5641 gtttcatcgg cggccttggc ggacgcgaca tcaccgacga ggagtttttt gaaatcgcac
     5701 gcgtcacccg ccaagcggtg gcacgcaacc agacgccgcc gcccaggctg ctgtacaacc
     5761 gcgacgaact catgcaaatt caaaagctcc aggccatcgc gcacgtggaa cgcgaagaac
     5821 tccgaaagaa tccatgagcg ataccgagac gctcttcaag cgcctccagg atttgccatc
     5881 gacccatctg ttgggcgggg gcacgtccat gtgcgcgggg tgcggcagct tgcaagccct
     5941 gcacgagatc tacgacatcc tcggcgccaa gaccgtgttc gtgaacgccg cgggctgcat
     6001 gaccctcttg tccacgtatc cgttcacgcc gtttcgcggg tcctggctgt acacggccat
     6061 ggcatccgcg cctgccggag cccaaggcgt tcgtgacgcg ttggacattc tcctcgaatc
     6121 gaatcgcatt ccgcccgaag agaacctgga cgtggtggtg ctgaccgggg acggctcggc
     6181 ctacggcatg ggcctgtccg ccacctccgc cgccatggag cggaacctgg atttcatata
     6241 catctgttat gacaacgagg gctacgccaa caccggccaa caagcctcgg gcgccacgcc
     6301 ccatgccgcc aaaaccgcca ccagcaccgg agcatgtggc ttttccgggt acaagaaaga
     6361 tctgttcgcg atctgggcgg cacacaagcc cgcgtacatc gccacggtga ttggtgcaga
     6421 ccccttggac ctggcgcgta agattgagaa agccaagcgc atgaaaggtc cgcgcatgat
     6481 catcgcgctc gcaccctgcc ccaccgggtg ggagta
//
LOCUS       DBNGTT010000458         4503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1257860, whole genome shotgun sequence.
ACCESSION   DBNGTT010000458 DBNGTT010000000
VERSION     DBNGTT010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1257860"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16BC_10380"
     CDS             complement(<1..233)
                     /locus_tag="AC16BC_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3242066.1"
                     /translation="MKEQINPAHIMQTATAFWASKVLLTAVELDVFTTLGDGSMTAQQ
                     LRDALGLHPRGTYDFFDALVALKFLIRDGDGPEG"
     gene            complement(511..819)
                     /locus_tag="AC16BC_10385"
     CDS             complement(511..819)
                     /locus_tag="AC16BC_10385"
                     /inference="COORDINATES: protein motif:HMM:NF048125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7128 family protein"
                     /protein_id="HIR3242067.1"
                     /translation="MGQKPCRARAETAIDSIRIFLGIPLGAKTIDFDTSGEAQRPITS
                     MQIIKRGGKTYYQCSECGLFYAEENWAGQCETWCKENNSCNLEIAKHAVCGSEELPDS
                     "
     gene            complement(957..2069)
                     /locus_tag="AC16BC_10390"
     CDS             complement(957..2069)
                     /locus_tag="AC16BC_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242068.1"
                     /translation="MDERVPLMDRILRAVVVLIGLVAVVAVSSRAVWAQEDRPEEPAA
                     PSAEDTPRPNGAVAAIDRAHEAISSRISETADRLDAFLGKARIQEESNQSYLNVALLR
                     TTDEHGVEITKQIKLKIVLPRLQRRLQLVIERDNDGGAIDSEGASGVVEDLTTGPSSG
                     DLTTALRLMVRSARDLNIYIDAGVRVRIHPTVFSRIRYRRSVELEQWAVRFTQSVKWE
                     EQFAENPYQWEVISRLDFDRPISPEYFFRSTLQGAWYEGRHGYFVTLGFALVQQITDD
                     RALVYEWNTLARTGQLVDTENNPPIILDPAGRFRIEETGVKLRYRQTIGWPWLFFEAD
                     AERAFRRYLDTYSDFDGFWRSLVKLEVQFREFGNTK"
     gene            2871..3224
                     /locus_tag="AC16BC_10395"
     CDS             2871..3224
                     /locus_tag="AC16BC_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013514.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242069.1"
                     /translation="MESVLEAGCPATRAISDQFGDEWFIQDALMHKGRAIQLDQRPRA
                     EENVAVAAASIMARAEFVRRLERLSEDAGLVLLKGASDLVEAAAKRLVRAKGEAALAA
                     FAKVHFKTTAKVLTK"
     gene            3259..4101
                     /locus_tag="AC16BC_10400"
     CDS             3259..4101
                     /locus_tag="AC16BC_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3242070.1"
                     /translation="MLTPQSKLPDPTQHVEQVKAFYANIAGLNLAQFPYFIRIYQLFD
                     TCQREIFAQGRHGGPYESVLDVGCGEGYHTFYVARFAKQVTGLDVSPDALALAEQRAR
                     ARAQRAVRVLGPDEAAAARRRVRLRDRPHPGLRRALAQLSRVCRPGALLVLDYDNQYH
                     PGLLWDAEERRLARADPEHGHTHAWSFRGRELQFNTFGRQEMRDLLARHGFRLERAYG
                     FDFFSFLIPKPERLQFSDHIGWPERAVMALNRLDIACRRWWPVSRWAYTQIGFARKTG
                     PGTP"
     gene            complement(4242..>4503)
                     /locus_tag="AC16BC_10405"
     CDS             complement(4242..>4503)
                     /locus_tag="AC16BC_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3242071.1"
                     /translation="RGSVTISVTAGEAPGEFILVVRDTGVGFPKEVDFHETSGSLGIR
                     LVLTLVSQLGGRIDLDRSSGTTVTIRFREQVHPQAAVGPLQS"
BASE COUNT          861 a         1411 c         1327 g          904 t
ORIGIN      
        1 ccctccggtc catcgccgtc acggatgagg aattttaagg ctaccagcgc atcgaaaaag
       61 tcgtatgtac cgcgtggatg aaggccaagt gcatctctga gttgctgtgc cgtcatcgac
      121 ccgtcaccaa gtgtggtgaa tacgtccagt tcgactgcgg tgagtagtac cttggaagcc
      181 caaaaggccg ttgcggtttg cataatgtga gccggattga tttgttcttt cattttcatt
      241 ctccctataa attgatgccc aacaaaaata ggctccctaa ttatggcgac aaatcaagcg
      301 tagaccgcct cctaaccgaa aaactgttgg gctgcagatt tctgcggtcg ctcttacttt
      361 gatatggtta cggcctatac ctctttcggt gattgacgca tgttatcgcg catcatacac
      421 gtccttaaac cccgcgcgca acgccttgac cagactgccc tcaatgctgg gctcgctgca
      481 tcccgccagc ggggtcaaaa tcgacacctt ttaagagtcc ggcagttcct cactcccaca
      541 cacggcgtgt ttagctattt cgagattgca actattattc tccttgcacc acgtttcgca
      601 ttgtccagcc cagttctctt ctgcgtagaa aaggccacat tcgctgcact gatagtacgt
      661 ttttcctccc cgcttaatga tctgcatcga agttataggc ctctgggctt cgcctgaggt
      721 gtcaaaatca attgtttttg cccctagggg aatgcctaaa aaaatccgta tggaatcaat
      781 ggctgtttcc gcccgggcgc gacaaggttt ttggcccatc tcctgggcct gtcaattcaa
      841 ggtgtgttgc ggcggagcac gtcgatgaac agggctcgac gtttggtcac ggagtgtcgg
      901 tgttggggtg atcgtagcgg attgattccg gcggggtcaa gcgttcgcct gacgggtcat
      961 ttggtattcc cgaattcccg gaactgcacc tcgagcttca ccagggaccg ccaaaagccg
     1021 tcaaaatccg aatacgtatc cagataacgg cgaaacgcgc gttccgcgtc cgcttcaaag
     1081 aacagccacg gccacccgat ggtctggcgg taccggagct tgacacccgt ctcctcgatg
     1141 cgaaaccggc cggccgggtc caggatgatc ggggggttgt tctcggtgtc cacgagctgt
     1201 cccgtccgcg ccagggtgtt ccactcgtac accaaggccc ggtcgtcggt tatctgctgg
     1261 accagcgcga atcccagggt gacgaaatag ccgtgccgtc cttcgtacca cgcgccttgg
     1321 agcgtggatc ggaaaaagta ctccggggag atcgggcggt cgaagtcgag ccgcgagatc
     1381 acttcccact ggtacgggtt ttccgcaaac tgctcttccc atttgacgct ttgcgtgaat
     1441 cgcacggccc actgttcgag ttcaacgctc ctccgataac ggatcctgga aaacaccgtg
     1501 gggtggatgc gcacccgaac acccgcgtca atgtagatgt tgagatcccg cgccgaacgc
     1561 accatcagac gaagcgcggt cgtcagatcg cctgatgacg gtcccgtggt caggtcttcg
     1621 acgactccgg acgcgccctc cgaatcaatc gcccctccgt cgttgtctcg ttcgatgacc
     1681 agttggaggc gccgttgcag cctggggagg acgatcttca acttgatttg tttcgtgatc
     1741 tcgacgccgt gttcgtcggt ggttcgcagg agggcgacgt tgagataact ttggttcgat
     1801 tcttcctgaa tacgcgcttt cccgaggaag gcatcgagac ggtccgcggt ttccgagatc
     1861 cgggaggaga tcgcctcatg cgcccggtcg atggcggcga ccgccccatt gggcctcggc
     1921 gtgtcctcag cggagggcgc cgccggttct tccgggcgat cctcttgagc ccacaccgcg
     1981 cgtgacgaga ctgcgaccac cgcgacgagc ccgatcagga caacgacggc tctcaggatt
     2041 cggtccatga gcggcacgcg ctcgtccaca tcacgcacgc gaggacgtgc cccgtctcgt
     2101 cggatatcgc agtcgtcccc ttaaaacccg accgacaact ggtgaaggcg cgcctaggtg
     2161 ggccattgcc gggtgatcat atctgacgcg gcgtctcggc gccaagcccg acgtgaccgg
     2221 gcaggaggct tagccgatcc tctcaggctc cgggagtgac ggtatccgct gagggttccg
     2281 gaagaactgt attagggaat cgatgcgatg gagccggcgg tcgggattga accgacgacc
     2341 tgctgattac gaacacaatt cagatccgac cagccccacc gatcctgacg aatcgccaac
     2401 aaatgcaaga ggtccgcccg gatgatcggt catcctcgtc tggtggactc agggtagttt
     2461 ggcaccacgc ttggcacaag ccctttgggg tgaacccctc gggtgagaca gtcttgaatt
     2521 ggcacacctg cagttggtgt agagtgtttc acccccctta gcctctttgc cttattaaat
     2581 ggttggtttc ttcacccatt ctctctcacg ggattgatga tctctggggg tttgaaaaac
     2641 ctcaacgtcg ctccggtagc acggggtgcc gagtgggtca cgtgttcaaa cgtctcgggc
     2701 gggcgtgaca gcaaacgtct ctccgaccgg acgagcgcgg acctcgccgc agacatcccc
     2761 ggactctgcc gcaccagcgt ggtcgcgatc ggccccgcca aatacaccga gctctacggc
     2821 aacttcaaga acaacaaccg gctcctcggg gggggggtca cgcgcgcgcg atcgaaagcg
     2881 tgctcgaagc cgggtgcccg gccacgcggg cgatcagtga tcaattcggc gacgagtggt
     2941 tcattcagga cgccctgatg cacaagggcc gcgccatcca gctggaccaa cgcccgcgcg
     3001 cggaggaaaa cgtcgcggtc gccgcggcgt cgatcatggc gcgggcggag ttcgtcaggc
     3061 gcctggaacg gctctcggaa gacgccggac tcgtgttgct caagggcgcg tcggatctcg
     3121 tcgaggccgc ggccaaacgc ctggtgcgcg ccaagggaga agcggcgctt gcggcgttcg
     3181 ccaaggtgca tttcaagacc acggccaaag tgctcacgaa gtgacttcgc caaggccctt
     3241 gcctatcatg ccgccccgat gttgaccccg cagagcaaac tgcccgatcc cacccagcac
     3301 gttgagcagg tcaaagcctt ctacgccaac atcgcaggcc tgaatctcgc gcagtttcct
     3361 tacttcatcc ggatctacca actcttcgac acctgtcagc gggagatctt cgcgcaaggc
     3421 cgtcacggcg ggccgtatga atcggtgttg gacgtgggct gcggcgaagg ctatcacacg
     3481 ttctacgtcg cgcgattcgc caaacaggtc acggggctag acgtgtcgcc cgacgcgctg
     3541 gccctggccg agcagcgcgc gcgagcgcgt gcccaacgtg cggttcgtgt gctcggacct
     3601 gacgaagccg ccgctgcccg acgccgcgtt cgactgcgtg atcggccaca tcccggacta
     3661 cgacgcgcgc tcgcgcagct ctcccgcgtg tgccgacccg gcgcgctcct ggtgctcgac
     3721 tacgacaacc aataccaccc ggggctgctc tgggacgcgg aggagcgccg tttggcccgg
     3781 gccgaccctg agcacggcca cacccacgcc tggtcgtttc gcggccgcga gctgcagttc
     3841 aacacgttcg gccgccagga aatgcgcgat ctgctcgcgc gccacggctt ccggctcgaa
     3901 cgcgcgtacg ggtttgactt cttctcgttc ctgatcccga agccggagcg tctgcaattc
     3961 tccgaccaca tcggatggcc cgaacgcgcg gtcatggcgc tgaaccgctt ggacatcgcc
     4021 tgccggcgct ggtggccggt tagccggtgg gcgtatacgc agatcggctt tgcccggaag
     4081 accggaccgg ggactccctg aaacgcgaag ggcgccccga caggacgccc ttcgacgcgg
     4141 tacggctgtc gagcaggcgt tgcgctacac acgccctcgg gtagacagct ccagcgtgcc
     4201 tccatcggcg gcaccaagta tgggactgaa gattacccag cttaggactg cagaggccca
     4261 acggcggcct ggggatggac ctgttcacgg aaccggatcg taacggtggt tccggatgat
     4321 cggtcgaggt cgatccgacc cccaagttgg ctcaccagcg tcaacacaag acgtatcccc
     4381 aacgaccccg aggtctcatg aaagtcgacc tccttgggaa atcccactcc cgtgtccctg
     4441 accaccaaga tgaactctcc gggcgcctct ccggccgtga cgctgatagt gacggaaccc
     4501 cgt
//
LOCUS       DBNGTT010000459         5843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1512660, whole genome shotgun sequence.
ACCESSION   DBNGTT010000459 DBNGTT010000000
VERSION     DBNGTT010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1512660"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="AC16BC_10410"
     CDS             <1..778
                     /locus_tag="AC16BC_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242072.1"
                     /translation="LKAAERLIDAASPKERIVIPGASSLPSMTAMLVQTAQAQWGPVD
                     RISIRVFIGNRNPKGRGAITYLIKALRTPFQAKRAGRWVQEHTWLDQRRAAGHFEARV
                     LPFGLVESPDDHFLPRWFGASDVSFRVSLEFAWIHGVIAGIGALQRALPPFCDRLWIN
                     ALFYGHPVIKAWGSRSGWLEVTTTHTSNGAARTAVQRLGAFDEGQRVPSFPMSIIGRV
                     LAGELARPSHTARRFDRLLSREQFLTALADEGIDYRVAVV"
     gene            789..1319
                     /locus_tag="AC16BC_10415"
     CDS             789..1319
                     /locus_tag="AC16BC_10415"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3242073.1"
                     /translation="MSSLRTVTVGLISDTHGLLRPEAVSALQGSDVIIHAGDIGGPDV
                     LDALSRVAPVTAVRGNNDKGSWASALPETDVLRIGATCMLVIHNLADLDRDPAAAGFH
                     VVVSGHSHRPGQRMEQGVPFVNPGSAGPRRFKRPVAAGRLLVADAHVTPELFELPADG
                     NTVSARPIISARRPLR"
     gene            1457..2194
                     /locus_tag="AC16BC_10420"
     CDS             1457..2194
                     /locus_tag="AC16BC_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3242074.1"
                     /translation="MAVRVFVVAAVIVVGWSAVNWAYQTVRKPTELFFPVSHALAKSP
                     PETWDRYGSLFAEHSTTVMTPEFLAALAQVEAAGNPVARTYWRWRLTTDPFEVYQPAS
                     SAVGMYQITDGTFREAKRYCIHDHVAVEDAPLEGARLCWFNGFYTRVLPSHAIEMTAA
                     LLDRTVARTMERRRIAAATLQQKQNLAALIHLCGAGAGDAYAGRGFRLTAGQRCGAHD
                     VGAYLARVNAAKRQFARLAAASASNKT"
     gene            2424..4778
                     /locus_tag="AC16BC_10425"
     CDS             2424..4778
                     /locus_tag="AC16BC_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family V/XII"
                     /protein_id="HIR3242075.1"
                     /translation="MNTRFVWLRRVSAVGCFVLALAFVPGSGFAATQDVGWPREIKSP
                     EGALVMYEPQIDSFEGTTVTGRAAVAATPAGKKDAVFGAVWFEWAVHVDRDTRTVEVL
                     DVKIPRSRFPGTSPDEEQKLADLVERNAKAGDLTIALDRLLAAVRASEREKRIAEGLN
                     NEPPKILFATEPTLLVVVDGEPQLADMQGVELMRVINTPYFLVFDPQAKRYWLNGGAV
                     WYRAADLKGEWTPSSVVPRAVSAAYRTMQEQVPDEERTPVVTTPSGAKPPKILVSFEP
                     AELIACAGEPTYTPIADNALLYVSNTDQDVFKEIATQDVYVLLSGRWFRGRSFDGPWE
                     FVRSDNLPPAFAEIPEGSKKGSVLAHVAGTPQADEAVLDAQVPQTAAIKRSEARTTVE
                     YDGEPRFQQIPNTEVEYAVNTSSQVLRIKGKYYVCDQAVWFVGPSPDGPWTLADQAPP
                     EVQAIPPDSPVYNVKYVYVYDATPDIVYVGYYPGYMGAYPYYGTVVWGTGWYYRPWIT
                     PYVYYPRPWSWGFHAHYNPYRGWIFGVGFASGPFYVSFWNGGGYGGYWGPSYYRPSQV
                     NIIHHVIVTRPAPAERRWTWGTNINVYNRPENRVRNAVVARERPSAARTRPITKPDNV
                     FADQDGRVYRRDKTGKWDTREGRQWKPAPIAPAQPRPGAGEIAPGPRTDPRPIAPPSR
                     PRSETRTFTPPPPGPGDSPARPRPESRASTPPPQPPPRAPSAKPERRFERAPTQSLQR
                     DFSARERGNARVQAFSKKSKERGSAGKERDGGGGIDFRRQPQGR"
     gene            4830..5081
                     /locus_tag="AC16BC_10430"
     CDS             4830..5081
                     /locus_tag="AC16BC_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3242076.1"
                     /translation="MCQGHAGPIDLLLTDVVMPRMSGLRLADRLRALRPQIKTLFISG
                     YLDDVLGEFGASAGDITLLQKPFAASALARKVREVPDRS"
     gene            complement(5151..>5843)
                     /locus_tag="AC16BC_10435"
     CDS             complement(5151..>5843)
                     /locus_tag="AC16BC_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3242077.1"
                     /translation="DQLVADRYAYFAGVPVALALGGGLLTGLTRRTVRTAAALAVMAA
                     VVILGLGASSWSLTRTWRDAGTLWARAVAVDPNSYQAHTNLGLYELSRNRYDAAVSEF
                     DAAIALNPRSSNARFDRGLALAKWGRTADAIASYQAGLALDPVDATAHAHLGDLFASI
                     ARWADAEAEYRAAARLVPHPDLYNSLGVALAQQDRFAEAVAAFRDALAMDPSHADAKA
                     NLDMALQIRPTR"
BASE COUNT          950 a         1923 c         2022 g          948 t
ORIGIN      
        1 tttaaaggcc gccgaacgac tgatcgatgc cgcgtcgcct aaggaacgga tcgtcattcc
       61 cggcgcctca agtcttccat ccatgaccgc gatgttggtg cagaccgcgc aggcgcaatg
      121 ggggccggtc gatcggatca gtattcgagt gttcatcggc aatcgcaatc ccaaaggcag
      181 gggcgcgatt acctacctca ttaaggcatt gaggaccccg ttccaggcga agcgggccgg
      241 ccgttgggtc caggaacaca cgtggctcga tcagcgccgc gcggcgggcc acttcgaggc
      301 ccgggtcttg ccgttcgggc tggtggagtc gcccgacgat cactttcttc cgcgctggtt
      361 cggcgcatcg gatgtcagct ttcgggtgag cctggagttt gcctggattc acggcgtcat
      421 cgcggggatc ggcgcgcttc agcgggccct gccgccattc tgtgaccgtc tgtggatcaa
      481 cgcgttgttc tacggccacc ccgtgatcaa ggcatggggc agccgttccg gatggttgga
      541 ggtcaccacg acgcacacgt cgaacggggc cgctcggacc gcggttcagc gcctgggcgc
      601 gttcgatgaa ggccagcgcg tgccgtcgtt ccccatgagt atcatcggac gggtcctcgc
      661 tggcgaactt gcgcggccct cacacacggc cagacgattc gatcgcctgc tgtccaggga
      721 acaatttctc accgccctgg ctgacgaagg tatcgattat cgcgttgcgg tcgtatagtt
      781 gggggcacat gtcttcgctg cgcacagtca cggtcggcct catctcggat acgcacgggt
      841 tgctgcggcc tgaagccgtc agtgcgttgc aaggtagcga tgtgattatc cacgcgggcg
      901 acatcggcgg cccggacgtg ctcgacgcgt tgtcgcgcgt ggcgccggtg acggcggttc
      961 gcggcaacaa cgacaaggga tcgtgggcct cggcgctgcc ggagaccgac gtgctgagga
     1021 ttggcgcgac ttgcatgttg gtgattcaca atctggccga cctcgatcgt gatcccgcgg
     1081 ccgccgggtt ccacgtggtg gtgtcgggtc actcgcaccg cccggggcag cggatggagc
     1141 agggcgtgcc gttcgtgaat cccgggagcg ccggtccgcg gcgcttcaaa cggcccgttg
     1201 cggctgggcg gctgcttgtt gcggatgctc acgtaacacc ggagttgttc gaattgccgg
     1261 cggatggaaa taccgtgagc gccaggccga tcatatcggc gcggagaccg ctcaggtgag
     1321 gcataatagg cgcatggcgt ccaaatcacg gtcgcgtgct cctaaagccg ctccccgaca
     1381 acggcggcgc gtccgtcggg tccgccgccg cgtgcgattg caccgacgcg tgctccgggc
     1441 gatcctggct gcgccgatgg cggttcgtgt ctttgtcgtg gcggcggtca tcgtggtggg
     1501 ctggtccgcg gtcaattggg cataccagac ggtgcgcaag cccaccgagt tgttctttcc
     1561 ggtcagccac gcgctggcca agagcccgcc cgagacgtgg gatcggtacg gatcgctgtt
     1621 cgccgaacat tcgaccaccg tgatgacgcc ggagttcctc gccgcgctcg cccaagtcga
     1681 ggccgcgggc aatcccgtgg cgcggacgta ctggcggtgg cgcctgacca cggatccctt
     1741 cgaggtgtac caacccgcgt cgagcgcggt ggggatgtac cagatcacgg acggcacgtt
     1801 ccgcgaggcc aagcgctact gcatccacga ccacgtggcc gtggaggacg cgccgctgga
     1861 gggcgcgaga ttgtgctggt tcaacggctt ctacacgcgc gtgctgccga gccacgccat
     1921 cgagatgacc gcggcgctgc tggaccgcac cgtggcgcgg accatggagc gccggcggat
     1981 cgccgcggcc acgttgcaac aaaagcagaa tctcgccgcg ctgatccacc tctgcggcgc
     2041 gggagcgggc gacgcgtatg ccggccgcgg gttccggctg accgcgggcc agcggtgcgg
     2101 cgcccacgac gtgggcgcct acctggcgcg ggtgaacgcc gcgaagcggc agtttgccag
     2161 gctggccgcc gcttcggcgt ccaacaaaac ctagccggcc gagcacggcg tagtcgtttc
     2221 cggttctccc gtcctcatgg ctttgcttgc gtccattcag agagtcacag taccattcgt
     2281 tgtccgttcc tccccacggt gttccgaggc gatacgcgca tgccgcgaca catggccgac
     2341 gccgacgtgt ttcagaacca ccccacaacg gctcacaccg tcgtctcaca acgtcgccga
     2401 aacggttgac ggaggttcca tccatgaaca cgcgtttcgt atggctgcga agggtatcgg
     2461 cggtcggctg cttcgtgctc gccctggcgt tcgtccctgg atcgggattc gcggcaaccc
     2521 aggacgtggg ctggccgcgg gagatcaaga gcccggaggg cgcgctggtc atgtacgagc
     2581 cccagatcga ttccttcgaa ggcaccacgg tgacgggccg ggccgcggtg gccgcgactc
     2641 ctgccggcaa gaaggacgcg gtcttcggcg cggtgtggtt cgagtgggcg gtgcacgtgg
     2701 accgtgacac ccgcaccgtg gaggtgctcg acgtcaagat tccgcgatcc aggttcccgg
     2761 ggacgagtcc ggatgaggag cagaagctcg cggatctcgt ggagcgcaac gccaaggcgg
     2821 gcgatctcac gatcgccctg gatcgcctgc tcgccgcggt gcgcgcgagc gaacgggaga
     2881 agcggatcgc cgaagggttg aacaacgagc cgccgaagat cctgtttgcc accgagccca
     2941 cgttgctggt ggtggtggac ggggagccgc agctcgcgga catgcagggc gtcgagttga
     3001 tgcgcgtgat caacaccccg tatttcctgg tgttcgaccc gcaggccaaa cggtattggt
     3061 tgaacggcgg cgcggtctgg taccgagccg cggacctcaa aggcgaatgg accccgtcca
     3121 gcgtggtccc tcgcgcggtg agcgcggcct accggacgat gcaggagcag gtcccggacg
     3181 aggagcgcac gcccgtggtc acgacgccgt ccggcgccaa gccgcccaag atcctggtgt
     3241 cgttcgagcc cgcggagttg atcgcctgcg ccggcgaacc cacctacacg ccgatcgcgg
     3301 acaacgcgct cctctacgtg agcaacaccg accaggacgt gttcaaggag atcgccaccc
     3361 aggacgtgta cgtgctgctc tcgggccgct ggtttcgcgg caggtcgttc gacggcccct
     3421 gggaattcgt gcggtccgac aacctgccgc ccgcctttgc ggagatcccc gaaggctcca
     3481 agaagggctc ggtcctggcg cacgtcgccg ggaccccgca ggcggacgaa gcggtcctgg
     3541 acgcgcaggt cccgcagacc gcggccatca aacggagtga agccaggacc acggtcgaat
     3601 acgacggcga gccccggttt cagcagatcc ccaacaccga ggtggaatac gcggtcaaca
     3661 cgtcatcgca ggtcctgagg atcaagggaa agtactacgt gtgcgaccag gcggtctggt
     3721 tcgtcgggcc cagccctgac gggccgtgga ctctcgcgga ccaggcgccg ccggaagtgc
     3781 aggcgatccc cccggacagc ccggtgtaca acgtgaagta cgtctacgtg tacgacgcga
     3841 ccccggacat cgtctacgtg ggctactacc ccggttacat gggcgcctac ccctactatg
     3901 gaacggtcgt gtggggcacc gggtggtact accggccgtg gatcacgccc tacgtctact
     3961 acccgcgtcc ctggtcctgg ggctttcacg cgcactacaa cccctaccgg ggatggatct
     4021 tcggcgtcgg cttcgcctcg gggcctttct acgtgagctt ttggaacggc ggagggtacg
     4081 gggggtactg ggggccgtcg tactatcgac ccagccaggt caatatcatc caccacgtca
     4141 tcgtcacccg ccccgcgccc gcggaacggc ggtggacctg ggggaccaac atcaacgtgt
     4201 acaaccgtcc ggagaaccgg gtgcgcaacg cggtggtggc gcgcgagcgg ccttccgcgg
     4261 cgcggacccg cccgatcacc aagccggaca acgtgttcgc ggaccaggac ggcagggtat
     4321 accggcgcga caagaccggg aagtgggaca cgcgcgaggg caggcagtgg aagccggccc
     4381 cgatcgcgcc ggctcagccg cgccccggcg caggggagat tgccccgggc ccgaggacgg
     4441 accccagacc cattgcgccg ccgtcccgcc cgaggtcgga gaccaggacg tttactccgc
     4501 cgccaccggg tcccggggac agccccgctc gtccaaggcc ggagagcagg gcgagcaccc
     4561 cgccgccgca accgccaccc agagctccgt ccgcgaaacc ggaacggcga ttcgagcggg
     4621 cccccacgca atccctgcag cgtgactttt ccgcgcgcga gcggggcaac gcgcgggtgc
     4681 aggccttttc caagaagtcg aaagaacgcg ggtccgccgg caaggagcgt gacggtggcg
     4741 gcggtatcga cttccggcgg caaccccaag ggaggtgaac gcggtcgtgg agtcaggccg
     4801 ggcgtgctgt tcgtgaatcc gggctccgcc tgtgtcaggg tcacgcgggg cccatcgatc
     4861 ttctgctcac ggacgtcgtg atgccgcgga tgagcggtct gcggctggcc gatcgcctgc
     4921 gcgcgttgcg cccgcagatc aagacgttgt tcatctccgg gtacctcgac gacgtgctgg
     4981 gcgagttcgg cgcgtcggcc ggagacatca cgctccttca aaagccgttt gccgcgtccg
     5041 cgctggcccg caaggtgcgc gaggtgccgg accggtcgtg atcgtccttc ggcccgtccg
     5101 cgtgggcacg gttcgcggtc gggtccgcgc catggtccgc cgaccggggg tcaccgagtc
     5161 ggcctgatct gcaacgccat gtccaggttc gccttggcat ccgcgtgaga gggatccatc
     5221 gccagcgcgt cgcgaaacgc ggcgacggct tcggcgaagc ggtcttgttg cgcgagcgcg
     5281 acgcccaggc tgttgtacaa gtccgggtgc ggaacgaggc gcgccgcggc ccgatactcg
     5341 gcctcggcgt cggcccatcg ggcgatcgag gcaaacaggt cgcccaggtg cgcgtgcgcg
     5401 gtggcatcca cagggtcgag cgcgaggccg gcttggtagg aggcgatcgc gtccgcggtc
     5461 cgtccccact tggccagggc caggccgcgg tcaaaccgcg cgttggacga acgggggttg
     5521 agtgcaatgg cggcgtcgaa ctcgcttacc gcggcgtcgt aacggttccg gctcaactcg
     5581 tagagcccca agttggtgtg ggcttggtac gagttcggat cgaccgcgac cgcgcgcgcc
     5641 cacagcgtcc cggcgtcgcg ccacgtgcga gtcagactcc aagagctcgc ccccagtccg
     5701 agaatcacca ccgccgccat caccgcgagc gcggccgccg tcctgaccgt acgtcgggtc
     5761 agccccgtca gcaggccgcc gccgagcgca agggccacgg gcacgccggc aaaataggcg
     5821 tagcgatcgg cgacgagttg atc
//
LOCUS       DBNGTT010000460         1934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2025570, whole genome shotgun sequence.
ACCESSION   DBNGTT010000460 DBNGTT010000000
VERSION     DBNGTT010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2025570"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="AC16BC_10440"
     CDS             complement(<1..300)
                     /locus_tag="AC16BC_10440"
                     /inference="COORDINATES: protein motif:HMM:NF018021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR3242078.1"
                     /translation="MTREEFKRLVSIALERVPDEFRMAMQNVQVVLDDWPEPALMEEV
                     TGDPDEVLYGLYTGTPLPDRTADYGNTTPDVITLYQGPLEEDFPDREELLYEIEVT"
     gene            complement(304..915)
                     /locus_tag="AC16BC_10445"
     CDS             complement(304..915)
                     /locus_tag="AC16BC_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242079.1"
                     /translation="MTVALACVFAPRTASGDAPGALLEDFASFPQQWKIKDDNPRAAD
                     IYRVVSDASGPYLTASVGDKPGVRIFKYLPWDTRAYPVVTWKWRLKSFPQDKRRSVAF
                     YVSIHRDSFGIPTIVKYLWSTQEPAGTIREGGFFGATEIVIQSGAAPDPEGWVTARVD
                     AVADFERIYGELPGPEGVGIGVTVGMGIEADIADVRVEPRAVN"
     gene            1147..>1934
                     /locus_tag="AC16BC_10450"
     CDS             1147..>1934
                     /locus_tag="AC16BC_10450"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR3242080.1"
                     /translation="MRSDNSEPARFAEVVVAAMPDRAYHYDIPAELARAVRPGVRVRV
                     PFGARVVDGYVVRLVARTDVDALKPVVDVLDEQPIIDATTLHFSHWIADYYLAPWGRV
                     LAAVVPVGLKARAVKRVAVTDAGRAMDPSSVRGDARRRLLAILSAQRGPITLARLERL
                     GGSPTALPALIQAGWVADHDGPPPKPSGRQRLYASLPPGGPASEVRVRGLKQQAVLDA
                     VTASGGAFVSDLGPGAAAVCRTLEAKGALVVSARESVQDPYRDVA"
BASE COUNT          302 a          623 c          675 g          334 t
ORIGIN      
        1 cgtgacttcg atctcgtaga gcagctcctc gcggtcgggg aaatcctcct cgagcgggcc
       61 ttgatacagg gtgatgacgt cgggcgtggt gttgccgtag tccgcggtcc gatccggcaa
      121 gggagtcccg gtgtagagtc cgtacagcac ctcgtcagga tcccccgtga cctcctccat
      181 gagcgcgggt tcgggccaat catcgagaac cacttggacg ttctgcatgg ccattcgaaa
      241 ttcgtcgggg acgcgctcaa gcgcgatcga gaccaggcgc ttgaactcct cgcgcgtcat
      301 gggttaattg acggcgcgtg gttccacgcg gacgtcggca atatcggcct cgatacccat
      361 gccgaccgtg acgccgatgc ccaccccttc cgggcccgga agttctccgt agatgcgttc
      421 aaagtcggcg acggcgtcca cgcgggccgt gacccatccc tcagggtccg gcgcggcgcc
      481 gctctggatc acaatttcag tggcgccgaa aaatccgccc tcccgaatcg tccccgcggg
      541 ttcctgcgtg ctccagaggt atttgacgat cgtgggaatc ccgaacgagt cgcggtggat
      601 ggagacatag aaggcgaccg accggcgctt gtcctgggga aaagatttga gccgccattt
      661 ccaggtgacc acgggatagg cacgcgtgtc ccacggcaga tatttgaaaa tccgaacccc
      721 gggtttgtcg cccacgctgg cggtcaaata cggacctgac gcatccgaga ccacgcgata
      781 aatgtccgcg gcgcgcgggt tgtcgtcttt gatcttccat tgctggggga acgacgcgaa
      841 gtcctcgagg agcgcgccgg gggcgtcgcc ggacgctgtc cgcggcgcaa acacgcacgc
      901 caaggcgacg gtcatgacca acagggctcg cgcaaccgcg ccgccggccc tcggaatcgc
      961 gttgaaaaac ataggaccga tggtagaaga atgccgttcg cgttgtcaac acgcgctcgg
     1021 ggcgcggaaa cggccggaag ggccgcgccg gtgagttctg gccacacgtc aaccgagtcg
     1081 gcgcgatgac ccgggaacgc gatgggcctg tgatacaatc cctcgctttc catgaattcc
     1141 cctcgcgtgc gttcggacaa ttcggagccg gctcggttcg ccgaagtggt ggtcgcggcg
     1201 atgccggatc gtgcctacca ctacgacatt cccgcggagt tggcgcgggc ggtgcgtccc
     1261 ggcgtgcggg tgcgggtgcc gttcggcgcc cgggtggtcg acggctacgt ggtgcggttg
     1321 gtggcgcgaa cagacgtcga cgccctcaag cccgtggtgg acgtgctgga cgaacagccc
     1381 atcatcgacg cgacgacctt acacttctct cactggatcg cggactatta cctcgcgccg
     1441 tgggggcggg tcctggccgc cgtggttccc gtcgggctca aggcgcgggc ggtcaagcgc
     1501 gtggctgtga cggacgcggg gcgcgcgatg gacccgtctt cggtgcgggg cgacgcgcgc
     1561 cgccggctgt tggcgatttt gtcggcgcaa cgcggaccga tcacgctggc gcggctcgaa
     1621 cgcctcggcg gttccccgac ggccctgccg gcgctgattc aagccggctg ggtggccgac
     1681 cacgacggcc cgccgccgaa gccgtcaggc cgtcagcggt tgtacgcgtc gctcccgccc
     1741 ggcgggcccg cctccgaggt tcgcgtgcgc gggctgaagc agcaggccgt gctcgacgcg
     1801 gtgacggcgt ccggcggggc gtttgtctcg gaccttggcc ccggcgccgc ggcggtgtgt
     1861 cgaaccctgg aggccaaggg cgcgctggtg gtttcggcgc gcgagtcggt tcaggatccg
     1921 taccgcgacg tggc
//
LOCUS       DBNGTT010000461         5473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2408048, whole genome shotgun sequence.
ACCESSION   DBNGTT010000461 DBNGTT010000000
VERSION     DBNGTT010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2408048"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1063
                     /locus_tag="AC16BC_10455"
     CDS             260..1063
                     /locus_tag="AC16BC_10455"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR3242081.1"
                     /translation="MAMNEDQIKRYSRHILLPEVGGKGQKKIANAKVFIVGAGGLGSP
                     AALYLAAAGVGTIGLIDGDVVDLSNLQRQVIHHTPDVSRPKIDSAREKMTQLNPDVTV
                     VPIYDRLTAKNALNAVKDFDIIIDGTDNFPSKFLINDTAVLAKKPLIHGGILRFVGQV
                     LTILPGQSACYRCLFKAPPPSGVVPSCQEAGVLGALAGLIGTIQATEALKLILGVGQP
                     LTDRLLTYDALATRFRNVPLRKNPQCPVCSEHPSITELVDYEQEVCRTA"
     gene            1083..2318
                     /gene="thrC"
                     /locus_tag="AC16BC_10460"
     CDS             1083..2318
                     /gene="thrC"
                     /locus_tag="AC16BC_10460"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3242082.1"
                     /translation="MDKIKGLKCRECGKLYRPDPLHVCEFCFGPLEVDYDYDAIATSI
                     TRDSITRGPKSLWRYIDLLPVEREATVGLHGGMTPMIRAHRLGQALGLDELYLKNDTV
                     NHPTLSFKDRVVAVALTRAREFGFDTVACASTGNLANSVSAHAAQAGFRCFVFIPSDL
                     ETGKVLGNLIYHPTVVAVEGNYDDVNRLCSEIAGEYRWAFVNINIRPYYAEGSKTLAF
                     ETAEQLGWRAPDQVVVPIASGSLLTKIWKGLKEFERVGLISGVKTKVNGAQATGCSPV
                     ATAFAEGRDFIKPVKPKTLAKSLAIGNPADGFYAVKTARETGGVITSVTDDEIVEGIH
                     LLARTEGVFAETAGGVTIGVLKKLVEQGAIARRDVTVAYITGNGLKTQEAVAGTLGEA
                     VYIEPSLEKFQRRFRLKEK"
     gene            2320..2598
                     /locus_tag="AC16BC_10465"
     CDS             2320..2598
                     /locus_tag="AC16BC_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3242083.1"
                     /translation="MAVTVRIPSPLRKLTGGASEIETKADSIVNLIDTLESEYPGMRE
                     RLCDESGTVRRFVNIYVNDEDIRFLSNQSTPLKDGDQVSIVPAIAGGA"
     gene            2607..2846
                     /locus_tag="AC16BC_10470"
     CDS             2607..2846
                     /locus_tag="AC16BC_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HIR3242084.1"
                     /translation="MASLKLHITFPENRIKDPIIYQIGHEFKVITNIRRSDVTGKISW
                     MDIELTGEVAEIERAVAALKSKGLKVDPIERNVIE"
     gene            2869..3690
                     /gene="moeB"
                     /locus_tag="AC16BC_10475"
     CDS             2869..3690
                     /gene="moeB"
                     /locus_tag="AC16BC_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR3242085.1"
                     /translation="MLTDSQIQRYSRHLILKEVGGKGQKKINDAKVFLVGAGGLGSPS
                     ALYLAAAGVGTLGIIDADTVDLSNLQRQILHSTDRVGVAKVESARQTLSALNPDITIN
                     VYQDRISSANIMDLIRDYDIVLDGSDNFPTRFLVNDAVFFQKKTLVSGSIFRFEGQVA
                     VVKAHQGTPCYRCLYPEPPPSGLVPSCQEAGVLGVLAGTIGILQAAETLKEILGVGES
                     LAQGLVMYDALDMSFRTLKTRKNPTCALCGPNPTITTLLDYEQSCTLPSAGAPHA"
     gene            3687..4085
                     /locus_tag="AC16BC_10480"
     CDS             3687..4085
                     /locus_tag="AC16BC_10480"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR3242086.1"
                     /translation="MKIPQPLVATMIAHAEEAYPHECCGLLAGANGVPSHVYRVQNVH
                     ETPRVFFEMAPKEQFWAFKNMRHNGQQLVAIYHSHPETPARPSQSDIRLAYDPDPFYL
                     LVSLQHRDKPDLRAYRIIDGSVTEEAIEVV"
     gene            4180..4545
                     /locus_tag="AC16BC_10485"
     CDS             4180..4545
                     /locus_tag="AC16BC_10485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3242087.1"
                     /translation="MTDQSGHEHGARWGDRLTVRQPVTIQWDDRDREGMIHHLSVSGC
                     FVQTGFPYPVGTTLHLSFGLGPDQSAFVTDGKVVMRNLGGIGVRFLYLHADDPAVLKR
                     WIDTHRHSPHADTAFPDAA"
     gene            4621..5196
                     /locus_tag="AC16BC_10490"
     CDS             4621..5196
                     /locus_tag="AC16BC_10490"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3242088.1"
                     /translation="MLTASTMLPIGTRAPDFSLADTNNRIVRRDDFTSAPALLVAFIC
                     NHCPYVKHIRTGLAEFSRQYQAKGLAIVGINSNDADEYPEDGPEHMVTEAKAAGYVFP
                     YLYDATQAVAKAYRAACTPDFFLFDARQRLVYRGQFDDSRPGNDAPVTGKDLRAACDA
                     ALAGKPIPATQKPSAGCNIKWKPGNEPDYFR"
     gene            complement(5204..>5473)
                     /locus_tag="AC16BC_10495"
     CDS             complement(5204..>5473)
                     /locus_tag="AC16BC_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011611556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3242089.1"
                     /translation="VLRDEGYGLIRWKQERQFGAPFGIDFGNPDLVKLAESFGAKGYR
                     VERADDFSDLLKDALARHVPAVIDVPIDYRDGMINPEQGKIICPT"
BASE COUNT         1086 a         1808 c         1662 g          917 t
ORIGIN      
        1 gcctgggcaa gggccggtgc gtggtcacgg tgctgccgga tcgcggcgag cggtatttca
       61 gcatggaaaa gtatttcggc acggcgtaga aggctgaccc aattattcga cttcgtcagc
      121 ccgttcggga acgcgccaga tgcaaggcgc cgcgagcacc gcaccggagc gtacgtggtg
      181 agtacgtgag gagcggatgc gcagcggcaa cgcagcagct ggccgttcat ggacgggccc
      241 ccagcagaga gtaacgacca tggcaatgaa cgaagaccag atcaaacgtt acagccggca
      301 catcctgctg cccgaagtcg gaggcaaggg gcagaaaaag atcgccaatg ccaaggtgtt
      361 catcgtcgga gccggaggac tgggatcacc cgccgcattg tacttggcgg cggccggggt
      421 cggcaccatc ggcctgattg acggcgacgt ggtggacctc tccaatctcc agcgccaggt
      481 gattcatcac acgcccgacg tgtcgcgccc caagatcgac tccgcgcgcg agaaaatgac
      541 gcaacttaat cccgacgtca cggtggtgcc gatctacgat cgcctgacgg ccaagaacgc
      601 gctcaacgcg gtcaaggatt ttgacatcat catcgacggc accgacaact ttccgtcgaa
      661 gtttttgatc aacgacacgg ccgtgctcgc gaaaaagccg ctgattcacg gcggcatcct
      721 acgcttcgtg gggcaggtgc tgacgattct acccggacag tcggcctgct atcgttgcct
      781 gttcaaggcg cccccgccgt ccggcgtggt gccctcctgc caggaggctg gggtgctggg
      841 cgcgttggcg ggcctcatcg gcacgattca ggcgacggaa gcgctcaagt tgattctggg
      901 cgtgggacaa ccattgaccg accggctctt gacctatgac gcgctggcca cgcggtttcg
      961 caacgtcccg ctgcgcaaga acccgcagtg cccggtgtgc agcgagcacc cgagcatcac
     1021 cgagttggtg gactacgagc aggaggtgtg tcggaccgcg taagacgccg accactgaac
     1081 ccatggacaa gatcaaaggc ctcaaatgcc gcgaatgcgg caagctctac cgcccggacc
     1141 cgctgcacgt gtgcgagttc tgcttcggcc cgctcgaagt cgactacgac tacgacgcga
     1201 tcgcgacgtc gatcacccgc gactcgatca cgcggggccc caagagtctg tggcgctaca
     1261 ttgacttgct gccggttgag cgcgaggcca cggtggggct gcacggcggc atgaccccca
     1321 tgattcgggc ccaccgcctg ggccaagccc tgggcctcga cgagttgtac ctgaagaacg
     1381 atacggtcaa ccaccccacc ctgtccttca aggatcgcgt ggtggcggtc gcgctcaccc
     1441 gcgcgcggga gttcggcttc gacaccgtgg cgtgcgcgtc caccggcaac ctggccaact
     1501 cggtctccgc gcacgcggcg caggcggggt ttcgctgctt cgtgttcatc ccatcggatc
     1561 ttgaaaccgg caaagtgctc gggaacttga tttaccatcc caccgtggtc gcggtggagg
     1621 gcaactacga cgacgtgaac cgcctgtgca gcgagatcgc gggcgaatat cgctgggcgt
     1681 tcgtcaacat caatattcgt ccctactacg ccgaaggctc gaaaacgctc gcgttcgaga
     1741 ccgcggaaca gctcggctgg cgcgcaccgg accaggtcgt ggtccccatc gcgtcgggct
     1801 cgttgttgac caagatctgg aaagggctca aggagttcga gcgcgtgggc ctgatctccg
     1861 gcgtgaagac caaggtcaac ggcgcgcaag ccaccggctg ttctcccgtg gccaccgcgt
     1921 tcgcggaagg acgcgatttc atcaagccgg tcaaacccaa gaccctggcc aagtcactgg
     1981 ccatcggcaa ccccgcggac ggattctacg cggtgaaaac cgcgcgcgag accggcggcg
     2041 tgatcacgag cgtgacggac gacgaaatcg tggaaggcat ccacctcttg gccaggaccg
     2101 aaggcgtgtt cgcggagacc gccggcgggg tgaccatcgg cgtgctcaag aaactcgtgg
     2161 agcaaggcgc catcgcccgg cgcgacgtca cggtcgccta catcaccggc aacgggctca
     2221 agacccagga agccgtggcc ggcacgctgg gtgaagccgt gtacatcgag ccctcgctcg
     2281 aaaaattcca acgacgattt cgactcaagg agaagtaaga tggcggtgac cgttcggatc
     2341 ccgtccccgc tgcgcaagct gaccgggggc gcaagcgaga tcgaaaccaa ggccgactcg
     2401 atcgtcaacc tcatcgacac gctggaatcc gagtaccccg gcatgcgcga gcgcctgtgc
     2461 gacgaatcag ggacggtgcg caggtttgtg aacatctacg tcaacgacga agacatccgt
     2521 tttctctcga atcagagcac gccattgaaa gacggggatc aggtctcgat cgtccccgcc
     2581 atcgcgggtg gagcctagcc cggaccatgg cgagcttaaa actgcacatt acattccccg
     2641 agaaccggat caaggacccg atcatttatc agatcgggca tgaattcaaa gtcatcacca
     2701 acatccggcg ctcggacgtc acggggaaaa tcagttggat ggacatcgag ttgaccggcg
     2761 aggtcgcgga gatcgagcgc gcggtcgcag cgctcaagag caagggcctt aaagtcgacc
     2821 ccatcgaacg aaacgtcatc gagtagttcc agcacggagc agacacgcat gttgaccgat
     2881 tcgcagatcc agcgctacag ccggcacctg atcctcaagg aagtcggggg caagggccaa
     2941 aaaaagatca acgacgccaa ggtgttcctg gtgggcgcgg gcgggctggg ctcgccgtcg
     3001 gccttgtacc tcgcggccgc cggcgtcgga accctgggca tcatcgacgc ggacaccgta
     3061 gatctttcca acctgcagcg gcagatcctg cactccacgg atcgcgtggg cgtggctaag
     3121 gtggagtccg cgcggcaaac gctctccgcg ctgaatcccg atatcacgat caacgtgtac
     3181 caggaccgga tctcgtccgc gaacatcatg gacctgatcc gcgactacga catcgtgctc
     3241 gacggatccg acaactttcc cacgcgcttt ctcgtaaacg acgcggtgtt cttccaaaag
     3301 aagaccctgg tgtcgggcag catctttcgt ttcgaaggcc aggtcgcggt cgtcaaagcg
     3361 caccagggca cgccttgcta ccgctgcctg tatccggagc cgcccccgtc cgggctggtg
     3421 ccctcgtgcc aggaagccgg cgtgctgggc gtgctggccg ggacgatcgg cattcttcag
     3481 gccgcggaga ccctcaagga aattctcggc gtgggtgaat cgctcgccca aggcctggtg
     3541 atgtacgacg cgctcgacat gtcgttccgc acgctcaaga cgcgcaagaa ccccacctgc
     3601 gcgctctgcg gcccgaaccc cacgatcacc accctgctcg actacgagca gtcctgtacg
     3661 ctgccgtccg cgggcgcgcc gcacgcgtga agattcccca gccccttgtc gcgacgatga
     3721 tcgcgcacgc cgaggaggcg tacccccacg agtgctgcgg tctgttggcc ggcgcgaacg
     3781 gcgtgccgtc gcacgtgtac cgcgtgcaaa acgtccacga gacgccccgc gtgtttttcg
     3841 aaatggcgcc caaggagcag ttctgggcct tcaagaacat gcgccacaac ggtcagcagc
     3901 tcgtcgccat ctatcattcc caccccgaaa ccccggcgcg gccgtcgcag agcgacatcc
     3961 gccttgccta cgaccccgac ccgttctacc tgctcgtctc gctccagcac cgcgacaaac
     4021 ccgacctgcg cgcctatcgg atcatcgacg gcagcgtcac cgaagaagcc atcgaagtcg
     4081 tctagcccgt tcgtcgcaag ggcgcctgac cgcgcaatag cggattctcc gctggcctgt
     4141 ttcatcgccg atggtatgct tgcgggcgag ggaacgggca tgaccgacca atcagggcac
     4201 gaacacggag cacgctgggg cgatcggctg acggtgcgcc agccggtcac catccagtgg
     4261 gacgaccgcg accgtgaggg catgatccac cacctcagcg tgtcgggctg ttttgtccag
     4321 accggattcc cgtaccccgt cggcaccacg ctgcacctct ccttcgggct cggccccgat
     4381 caatccgcgt tcgtgaccga cgggaaggtc gtcatgcgaa atctgggcgg catcggagtg
     4441 cgcttcctgt acctgcacgc ggacgacccg gcggtattga agcgatggat cgacacccat
     4501 cgccattcgc cccacgccga caccgcgttt cccgacgcgg cttgacagcg gaccttcgcg
     4561 cttcttattg tcgctgacgc gcgccgcgcc tcagccatcc catccgcaag gagacgtttc
     4621 atgttgactg cgtcgaccat gttgccgatc ggcacccgtg ccccggactt cagcctggcg
     4681 gacacgaaca accggatcgt ccgccgcgac gatttcacct ccgccccggc cctgctcgtg
     4741 gcattcatct gcaatcactg cccgtacgtc aaacacattc gcaccggatt ggccgagttc
     4801 tcacgccagt accaggccaa ggggctcgcg atcgtgggaa tcaacagcaa cgacgcggac
     4861 gagtacccgg aagacggccc ggagcacatg gtgaccgagg ccaaggcagc gggatatgtg
     4921 tttccctacc tgtacgacgc cacccaagcc gtggccaaag cctaccgcgc cgcatgtacg
     4981 ccggacttct ttctcttcga cgcccgccag cggctcgtgt accggggcca gttcgacgac
     5041 agccggcccg gcaacgacgc gcccgtgacg ggcaaggacc tccgcgccgc gtgcgacgcc
     5101 gcgctggccg gcaaacccat tcccgccacg cagaagccca gcgcaggatg caacatcaag
     5161 tggaaaccgg gaaacgaacc cgactacttc cgttgacggt tgcttaggtg gggcagatga
     5221 tcttgccttg ctccggattg atcatgccat ctcggtagtc gatcggcacg tcgatcaccg
     5281 cgggcacgtg ccgggccaag gcgtctttta agaggtcgct gaaatcgtca gcccgctcca
     5341 cgcgataccc tttggcgccg aacgactccg ccagtttcac cagatccgga ttcccgaagt
     5401 cgatgccgaa cggcgcgccg aactgacgct cctgtttcca gcggatgagt ccgtatccct
     5461 cgtcgcgcaa cac
//
LOCUS       DBNGTT010000462         7206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_109972, whole genome shotgun sequence.
ACCESSION   DBNGTT010000462 DBNGTT010000000
VERSION     DBNGTT010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_109972"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="AC16BC_10500"
     CDS             <1..607
                     /locus_tag="AC16BC_10500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR3242090.1"
                     /translation="FRTLKEQLGVPGEVSLDLLISARDVLTTAEPETDVEGLADHVEG
                     LIQRALDGLDAMRRQEGESIARDVTGRLNQVASILDRVAVRAPRIAEEYAVKLRARVA
                     SLAGGMDVDPSRLAQEVALYAERCDFSEEVTRIRSHLDQFSKSMTGGGAVGRSLDFLT
                     QELNREINTIGSKANDAEVAAAVVALKSELEKIREQIQNIE"
     gene            812..1462
                     /gene="gmk"
                     /locus_tag="AC16BC_10505"
     CDS             812..1462
                     /gene="gmk"
                     /locus_tag="AC16BC_10505"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3242091.1"
                     /translation="MTTGPVGSSQGKGLLFVVSAPSGAGKTTLCREVANLVPGLRHSV
                     SYTTRKPRPGEVDGRDYFFLDDGGFRKKAERGEFIEWAEVHGNLYGTPWEQILMHDEQ
                     GVDLILDIDTQGAMQLKKKEVEGVYIYILPPSFDVLKARLLERKSDSPEEIARRLRKA
                     REEIWSYREYYYLIINDDVKRAMLELQSIIISERIKMKRMNFSWIEDTFIKNKEVF"
     gene            1464..1832
                     /gene="rpoZ"
                     /locus_tag="AC16BC_10510"
     CDS             1464..1832
                     /gene="rpoZ"
                     /locus_tag="AC16BC_10510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3242092.1"
                     /translation="MEIIELPINLDEISLDSRYRMVVIGAQRARQLMEGARASETTKY
                     SKATTVALEEFLASELKYVTGKDARLAQREARKKREELMRRRSLLEREEEISAEIKKD
                     LSVYIDDSAKRAAESPESPA"
     gene            1843..3045
                     /gene="coaBC"
                     /locus_tag="AC16BC_10515"
     CDS             1843..3045
                     /gene="coaBC"
                     /locus_tag="AC16BC_10515"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR3242093.1"
                     /translation="MLNGRRILLGVSGSIAAYKSVALLRRLTAMGADVTVIMTEAAKR
                     FVGPLTFQTLSGHPVYHDLFEPREEILHLTLADTADVFLIAPATANTIAKLAAGSADD
                     LLSALALAARGPVVLAPAMDAVMWEHRLVQRNLTALREAGVTVVPPESGALASGLVGP
                     GRLASEEAIIAAVTDRLGAGGRWSRERVLVTAGPTSEPIDPVRTITNRSSGKMGYAVA
                     AAAVRRGAQVTLISGPTRMAPPPGVAVVLVETADEMRRAVLEHADQATILIMAAAVAD
                     FRPKTVHAGKMKKRSAPVTLELVPTEDILGAVARRRGELFVVGFAAETDRVVEHASEK
                     LKTKHLDLIVANDVSIPGIGFGADDNEVTLIDRHGGIMPLPRLPKSAVADRILDHVEA
                     LRQPLADD"
     gene            3161..4270
                     /locus_tag="AC16BC_10520"
     CDS             3161..4270
                     /locus_tag="AC16BC_10520"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3242094.1"
                     /translation="MEGCLPGKDDTRAPELQKLEEKLRKDPASKLFFPLAEEYAKIGR
                     LDEAIALLRTGVKAHPDFLGARVALGKVLLTKGQTAEAKKEFEQVVAANPDNLMAHKK
                     LAAIYFKDQDRDKALASCEIVLAVSRTDPEALKLREEISRLPVPSAEEEPTELARPAT
                     LDPPEAEPTVVEAIRPAEEDVEATVTMVEPTVVERTVVEPPAEPTVVESIPAEPEVAE
                     VTEAIELPREAPMFPDLEAAPDRSAGNEMGDEDLATLSLADLYVAQGHYDRGIAIYRR
                     LLERTPGDADLSAKLDNALTLERLLAPQAVDAEVVKSEVVSQVLSRGEEPEIESLGEA
                     SHPPLVGVAANVARNQAAIRRLEVWLTRITERRRR"
     gene            4267..4623
                     /locus_tag="AC16BC_10525"
     CDS             4267..4623
                     /locus_tag="AC16BC_10525"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR3242095.1"
                     /translation="MSLAASLQSILNDMEGAEGIALIGVDGIIVNEVKRSPEVDLNTV
                     AAEYCGLWREMDRVSGGLSFGSTEECSISSEGHTLLFRRVTPGYFLALSMGRDGNAGK
                     GRFLLRYAAPQLARDL"
     gene            4650..5111
                     /gene="aroQ"
                     /locus_tag="AC16BC_10530"
     CDS             4650..5111
                     /gene="aroQ"
                     /locus_tag="AC16BC_10530"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008275861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3242096.1"
                     /translation="MRILVLHGPNLNLLGVREPELYGSLSLDGINDGIRAAAKDAGAN
                     VEIRQSNSEGELVTWIQEAPKRFDALVMNAAAYTHTSIAIRDAIAAVRIPTVDVHLSN
                     IHAREPFRHASRVAEVAVGQISGFGAYSYVLGIEAAVHHLRSRSESPASGV"
     gene            5157..5723
                     /gene="efp"
                     /locus_tag="AC16BC_10535"
     CDS             5157..5723
                     /gene="efp"
                     /locus_tag="AC16BC_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3242097.1"
                     /translation="MIVTTDFRNGLKLEVDGEPMEIIEYQHVKPGKGGAFVRTKMRNL
                     KTGNVLERTFRSGEKFEDPQVEERKMQYLYLQDGNYHFMDTDSYEQLFLSKEQMGDSW
                     QFLKENMEVDILFCKGKPLGVSLPLFVELTIVDTAPGIRGDTATGGTKPATLESGGVV
                     KVPFHIGPGTVIKVDTRTKAYVERVKGP"
     gene            5720..6169
                     /gene="accB"
                     /locus_tag="AC16BC_10540"
     CDS             5720..6169
                     /gene="accB"
                     /locus_tag="AC16BC_10540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3242098.1"
                     /translation="MNLREIKELIDLVKTSELTELEWERSGVRVRICRAPAPMVRHLA
                     TEGAVTAVNVPQGAPPAAGEAAKEVSGVQIRSPIVGTFYRAASPEAPPYVEVGDRVRR
                     GQILCIVEAMKLLNEIESEVDGVVAEILIENARPVEYGEVLFRIEPT"
     gene            6376..>7206
                     /locus_tag="AC16BC_10545"
     CDS             6376..>7206
                     /locus_tag="AC16BC_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3242099.1"
                     /translation="MAGLFKKILIANRGEIALRIIRSCRDLGIQTVVAYSEADAESLP
                     VQIADQRVCIGPPDPGMSYRNIPNILSAAEITGAEAIHPGYGFLAENAQFAEACEAAG
                     FKFIGPSPESIALMGDKAKARELMIKHGVPVIPGSTGALRDEAEARETAKRIGYPVIV
                     KASAGGGGRGMRVVNQEDDLLKAIQTAQTEAKSAFGDDSVYLEKYFIDPRHIEVQILA
                     DKKGHTIALGERDCSIQRRHQKLIEESPSPAVSPELRREMMKAAVDAVKAAKYVNTGT
                     V"
BASE COUNT         1394 a         2085 c         2494 g         1233 t
ORIGIN      
        1 gtttcgaacg ctcaaggagc aactgggcgt gccgggggag gtgtcgctgg atctgctgat
       61 ttccgcgcgg gacgtgctga cgacagccga acccgaaacc gacgtggagg ggctggccga
      121 ccacgtggaa gggttgatcc aacgggcctt ggatggcctg gatgcgatgc ggcgtcagga
      181 aggcgagagc atcgcgcggg acgtgaccgg gcggttgaat caggtcgcgt cgatcctgga
      241 tcgggtggcg gtccgcgcgc cgcggatcgc ggaagagtac gccgtgaagc tccgtgcgcg
      301 cgtcgcgtcg ctggccgggg gtatggacgt ggatccgtcg cgtctggcgc aggaagtggc
      361 cctgtacgcc gagcggtgcg acttttcgga agaggtgacc cggattcgca gccacctcga
      421 ccagttttcg aagtcgatga ccggtggggg cgcggtcggg cggtccctgg attttttgac
      481 gcaagaattg aatcgagaga tcaatacgat cggttccaag gccaacgacg cggaggtggc
      541 ggccgcggtc gtggcgctga agagcgagtt ggagaagatt cgcgagcaga ttcaaaacat
      601 cgagtgactc ggatgcgaag gaatccgctg gttcgaaaat ccgaacgaac ggacatcctc
      661 cagaaggtag acactacgtg ttccggagcg taaagcaaat ccccccttac ccccctttgc
      721 caaagggggg atggggggga tttcagacag gcgatgtggt cagcccggtc gccgaattga
      781 tggactcctt agcaagctgc cggggggcac ggtgacgacg gggccggtgg ggtccagcca
      841 agggaaaggc ttgttgttcg tggtctccgc gccgtcgggc gcggggaaga cgaccttgtg
      901 ccgggaagtc gcgaacctcg tcccgggtct acgccactcc gtgtcctata ccacgcgcaa
      961 gccgcggccc ggcgaagtgg acgggcggga ctacttcttc cttgacgacg gcgggtttcg
     1021 caagaaggcg gagcgggggg aatttattga atgggcggag gtgcacggga acctgtacgg
     1081 cacgccgtgg gaacagatcc tcatgcacga cgaacagggc gttgatttga tcttggatat
     1141 cgacacccaa ggcgcgatgc agctcaagaa aaaggaggtc gagggggtct atatctacat
     1201 ccttcccccc tcgtttgacg tgctcaaggc gcgcctcctg gagcggaagt ccgatagccc
     1261 cgaggaaatc gctcggagac ttcgaaaagc ccgggaggaa atttggagtt atcgagagta
     1321 ttattatctc atcatcaacg acgatgtgaa gcgggccatg ctcgaactgc agtcgatcat
     1381 catttcggaa cggatcaaga tgaagcgcat gaatttttcg tggatcgaag acacgttcat
     1441 caagaataag gaggtattct gacgtggaaa tcatcgaact gccgatcaat ctcgacgaga
     1501 tctccctgga ttcccgatat cgcatggtcg tgatcggcgc gcaacgcgcc cgccaactga
     1561 tggagggcgc gcgcgccagc gagaccacga agtattccaa ggccacgacc gtggcgttgg
     1621 aggagttcct ggccagcgaa ttgaagtacg tcacgggcaa ggacgcgcgt ctggcccaac
     1681 gcgaagcgcg caagaagcgc gaggagttga tgcggcggcg gtcgcttttg gagcgagagg
     1741 aagagatcag cgccgagatc aagaaggacc tgtcggtcta catcgacgat tccgccaagc
     1801 gcgcggccga gtctccggaa tcgccggcgt agcgacgtcg gtatgctgaa cgggcgacgc
     1861 atcctgctcg gggtttcggg gagcatcgcc gcctataagt cggttgcgct gcttcggcga
     1921 ctgacggcca tgggcgcgga cgtgaccgtg atcatgaccg aggccgcgaa gcggttcgtc
     1981 ggtccgctca cctttcaaac cctctccggc cacccggtgt accacgacct gttcgagccg
     2041 cgggaggaga tcctccacct gacgttggcc gacacggccg acgtgttcct gatcgctccg
     2101 gccaccgcca ataccattgc caaattggcg gccggttcgg cggatgacct gctgtccgcg
     2161 ctggcgctcg cggcccgtgg accggtcgtg ctggcgccgg ccatggatgc ggtgatgtgg
     2221 gaacaccgcc tggtccaacg aaacctcacc gccctgcgtg aggcgggggt gacggtggtg
     2281 cctccggaga gcggggcgct ggcctcgggc ctggtgggcc ccgggcgact cgcctccgag
     2341 gaggcgatca tcgcggcggt caccgaccga ttgggagccg gcgggcgctg gtcgcgcgaa
     2401 cgcgttctcg tgaccgcggg ccccaccagc gagccgattg atccggttcg caccatcacc
     2461 aaccgatcat ccgggaaaat ggggtatgcc gtggccgcgg ccgcggttcg gcgcggcgcg
     2521 caggtgacgc tcatcagcgg gccgacgcgg atggctccgc cgccgggcgt cgcggtggtt
     2581 ctggtcgaga ccgcggacga gatgcggcgg gcggtgctcg agcacgcgga tcaggcgacg
     2641 atcctgatca tggcggcggc cgtggcggat ttccgcccca aaacggttca tgccggcaag
     2701 atgaagaagc ggtccgcgcc ggtgacgctg gaactcgtgc cgacggaaga cattctgggc
     2761 gcggtcgcgc ggcgccgcgg cgaactcttc gtggtggggt ttgcggcgga gaccgaccgc
     2821 gtggtggagc acgcctcgga gaagctcaaa accaagcacc ttgacttgat cgtggcgaac
     2881 gacgtgtcga tcccggggat cggattcggg gcggacgaca acgaggtcac gttgattgac
     2941 cgccatggcg ggatcatgcc gttgccgcga cttcccaaat cagcggtggc ggatcggatc
     3001 ttggaccacg tcgaggcgct tcggcagccg ttggccgacg actgacggtt tggcgccgcc
     3061 aggctcacgg cctcgatcga ccgatctggt tgactttctt catgaagttg gtaagatgag
     3121 ggccgtttcc tcccgcctgt gacgcgtgag cgggtaactc atggagggct gtttgccggg
     3181 caaggacgat actcgggccc ccgagctcca aaagctcgaa gaaaagctcc ggaaggatcc
     3241 cgcgagcaag ctgttcttcc cgctggccga ggaatacgcc aagatcggac ggctggacga
     3301 agcgatcgcg ctgcttcgga ccggggtcaa ggcgcacccg gatttcctcg gggcccgcgt
     3361 ggcgctgggg aaagtcctcc tcaccaaagg ccagactgct gaagccaaga aggagttcga
     3421 gcaggtggtc gccgcgaacc ccgacaacct catggcccac aaaaaactcg cggccatcta
     3481 cttcaaggat caggataggg acaaagcgtt ggcctcgtgc gagatcgtct tggcggtcag
     3541 tcggaccgac cctgaggcgc tcaagctccg ggaggagatc agccggttgc cggtcccgtc
     3601 cgctgaagag gagcccaccg agctggcgcg accggccacg ctcgatccgc ctgaggctga
     3661 acccacggtg gtggaggcga ttcgtccggc ggaagaagac gtcgaggcga ccgtgacgat
     3721 ggtcgagccc accgtggtcg aacgcacagt ggtcgaaccg ccggcagaac cgacggtcgt
     3781 ggagtcgatt cccgccgaac ccgaggttgc cgaggtcacg gaagcgattg aactcccgag
     3841 ggaggcgccc atgtttccgg acctggaggc agccccggac cgctccgccg ggaacgaaat
     3901 gggcgacgag gatttggcca cgctctctct ggcggatttg tacgtggcac agggtcatta
     3961 cgaccgcggg attgcgattt atcgacgcct gctggaacga acgccgggcg atgcggatct
     4021 ttccgcaaag ctggacaatg ccctcacgtt ggaacgcttg ttggccccgc aagcggtgga
     4081 cgcggaggtg gtcaagtccg aggtggtgtc tcaagtgttg agcaggggcg aagaaccgga
     4141 aatagaatcc ttgggcgaag cgtcgcaccc gccgttggtc ggcgtggccg cgaacgtggc
     4201 ccggaatcag gccgcgatcc gccggcttga ggtgtggttg acccgtatca cagagaggcg
     4261 ccgccgatga gtctcgccgc atcgctacag tctattctca acgatatgga aggtgccgag
     4321 ggcattgcgt tgatcggcgt ggacggcatc atcgtcaatg aggtgaagcg gtcgccggag
     4381 gtggatctca acaccgtggc tgcggaatat tgcggtctct ggcgggagat ggaccgcgtc
     4441 tcgggcggcc tgtccttcgg ttcgaccgag gagtgttcga tttccagtga gggtcatacc
     4501 ctcttgttcc ggcgcgtgac gccgggatac tttctcgcgc tgtccatggg gcgagacggc
     4561 aacgcgggca agggacgctt cttgctgcga tacgccgcgc cccaactggc cagggacctg
     4621 tgaccggtcg cctcgcgcgg aggcggctga tgcggatcct ggtgctgcac ggccccaacc
     4681 tgaacctgct cggcgttcgg gagcctgagc tgtacggttc gctgtcgctt gacgggatca
     4741 acgacgggat tcgggccgcg gcgaaggacg cgggggcgaa tgtcgagatc cgccagtcga
     4801 atagcgaggg cgagttggtg acgtggatcc aggaggcgcc caagcgtttc gacgccctgg
     4861 tcatgaacgc cgcggcctac acgcatacga gtatcgcgat ccgcgacgcc atcgccgcgg
     4921 tgcggattcc gaccgttgat gtgcacctgt ccaatatcca cgcacgggag cccttccgac
     4981 acgcctctcg tgtcgcggaa gtggcggtgg ggcagatcag cggattcggc gcctacagct
     5041 acgtgctggg catcgaggcc gcggtccatc atctccgtag tcgttccgag agccccgctt
     5101 ccggggttta actgagtcgg ttgtatcagc ctgtcgagtg tgaaggagac gcgtacgtga
     5161 ttgttaccac tgattttcgt aatgggttga aattggaggt agacggcgaa ccgatggaaa
     5221 tcatcgagta tcagcacgtc aaacccggta agggcggggc gttcgtccgg accaagatgc
     5281 gcaacctcaa gaccgggaac gtgttggagc ggacgtttcg ctcgggcgag aaattcgaag
     5341 acccgcaagt cgaagagcgg aaaatgcagt atctctatct ccaagacggg aactaccact
     5401 tcatggacac ggattcgtac gaacagttgt tcctgtccaa ggaacagatg ggagactcct
     5461 ggcagtttct caaggagaac atggaggtgg acatcctttt ctgcaagggg aagccgctcg
     5521 gcgtcagctt gccgctgttc gtggagttga cgatcgtgga cacggcgccc ggcatccggg
     5581 gcgataccgc gaccggcggc accaagcccg ccacgctgga gtccgggggg gtcgtgaagg
     5641 tgccgtttca catcgggccg ggcaccgtca tcaaggtcga tacacggacc aaggcgtacg
     5701 tcgaacgggt caaggggcca tgaacctgcg cgagattaaa gagctcatcg atctggtcaa
     5761 gaccagcgag ttgaccgagc tggaatggga gcgatccggc gtgcgcgtgc ggatttgccg
     5821 ggcgccggcg ccgatggtcc ggcatctggc caccgaggga gcggtgacgg cggtgaacgt
     5881 gccgcagggc gcgccgcctg ccgccggcga ggcggccaag gaagtcagcg gggtgcaaat
     5941 ccgctcgccg atcgtcggga ccttctaccg cgccgcatcg cccgaggcgc cgccatacgt
     6001 ggaggtcggc gaccgggtgc gacgcggtca gattctgtgc atcgtcgaag ccatgaagct
     6061 gctcaacgag atcgagtccg aagtcgacgg ggtggtcgcg gaaatcttga tcgagaacgc
     6121 gcggcccgtt gaatatggag aagtcctctt ccgcatcgaa cccacatgag agcctgtcca
     6181 tgaggggtca tctgcggcgt tgtcgctgcg cttccggtgc tcacgtacac acagagtacg
     6241 ctccgctccg gtgctcgctc cgccttgcga cccaccgcca agaattcgaa tggcggaagt
     6301 ccccgaaggg ggcatctgac gccctcctga acaggctccg agggtagtgc cctcagacag
     6361 gaccaaaaga aaaccatggc agggctcttc aaaaagatat tgatcgccaa ccgcggcgaa
     6421 atcgcgctgc ggatcatccg ctcgtgccgc gacctcggga ttcagaccgt ggtggcgtat
     6481 tccgaggccg acgccgagag cctcccggtt cagatcgccg atcaacgggt ctgtatcggt
     6541 cccccggatc cgggaatgag ttaccggaac attccgaaca tcttgagcgc ggccgaaatc
     6601 acgggcgcgg aagccatcca tcccgggtac gggttcctgg ccgagaacgc ccagttcgcc
     6661 gaggcgtgtg aggcggcggg gttcaaattc atcgggccgt cgcccgagag catcgcgttg
     6721 atgggcgaca aggccaaggc gcgtgagttg atgatcaaac acggcgtgcc cgtgattccc
     6781 ggaagcacgg gcgcgttgcg agacgaggcg gaggcgcgcg agacggccaa gcgcatcggg
     6841 taccccgtga tcgtgaaggc ctccgcgggc ggcggcggac gggggatgcg ggtggtcaac
     6901 caggaggacg acctcctcaa ggcgattcag accgcgcaga ccgaggccaa gtccgcgttc
     6961 ggagacgact cggtgtacct ggagaagtac ttcatcgacc cgcggcacat cgaggttcag
     7021 atcctggccg acaagaaggg tcacaccatc gcgttgggcg agcgggactg ctccattcaa
     7081 cggcgccatc agaaactgat cgaagagtcg ccgtcaccgg ccgtgagccc ggagttgcgc
     7141 cgcgagatga tgaaggcggc ggtggacgcg gtcaaggccg ccaagtacgt caacaccgga
     7201 accgtc
//
LOCUS       DBNGTT010000463         2312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1004652, whole genome shotgun sequence.
ACCESSION   DBNGTT010000463 DBNGTT010000000
VERSION     DBNGTT010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1004652"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="AC16BC_10550"
     CDS             <1..693
                     /locus_tag="AC16BC_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3242100.1"
                     /translation="FGVSGRLAKAFLDGRGAGQPLQSGIDLRLTTSAIRDGFLLCQTF
                     LGPKIHAPNYAEPDLATVLARVVGTLFECMGTDEAFWLSRTVSAPLPTFGFQYDLSLE
                     PVRVNRDRMLQVFRNGVREFASILSPILSPATLEAIQRLIDVSDADLRFPDELWVKTI
                     YEFAASYHHGVIHRDHLLQALVPLYLGRTSSFVAENLGTDSMEFEQRLEALCLEYERF
                     KPYLIERWKPTT"
     gene            701..1402
                     /locus_tag="AC16BC_10555"
     CDS             701..1402
                     /locus_tag="AC16BC_10555"
                     /inference="COORDINATES: protein motif:HMM:NF017373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3242101.1"
                     /translation="MTETLLNGVTQTLDAALKMGIELLPKILGMLIIIAIGWVIAIVV
                     RLAVRRLLSLVKFNALCENVGATQIAAKADLPPPVDLLSALVFWVTWAAFLMLGISTL
                     GIVVLQEQIAEFFRYVPQIFIAVVILFMGLLFANFTARAVLLGMVNANVPSARLLSNA
                     VRVFLVMLSVVMALEQIALAKTVVLIAFSIAFGAVMLGLAIAFGLGGRDVARRMLERQ
                     FTEDAKSEKDEISHL"
     gene            complement(1391..>2312)
                     /gene="nhaA"
                     /locus_tag="AC16BC_10560"
     CDS             complement(1391..>2312)
                     /gene="nhaA"
                     /locus_tag="AC16BC_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR3242102.1"
                     /translation="TDIAFALGILTLLGKRVPLSLKVFLTALAIVDDLGAVVVIALFY
                     TDTIHWGSVFPALVILAVLMELNRQGVRHPLVYGVLGLGLWSAVLSSGIHATIAGVAL
                     AMTIPSRARLASDQFLAQSRATLAEFERAGHSREDQFITEAHQSALQALKSAAIQVQT
                     PMERLEHALHPAMRFVVMPLFALANAGVSFSGLGLSMLADPVVLGVFFGLVLGKQLGI
                     FLCAWGAVRSGLAALPPDLRWHHVYGAAWLGGIGFTMSLFIANLAFAETPFLPIAKLG
                     ILGASLVAGGVGWAILRGTGGRGARASSEV"
BASE COUNT          428 a          767 c          718 g          399 t
ORIGIN      
        1 tttggtgtct ccggacgcct cgccaaggcc tttctcgacg ggcgcggcgc gggacagccg
       61 cttcaatcgg gcatcgatct ccggctcacc acgtcggcca tccgggacgg gttcctgctc
      121 tgccagacgt tcctcgggcc gaaaatccac gcacccaact acgcggagcc ggatctcgca
      181 accgtgctgg cgcgcgtcgt cggcacattg ttcgagtgca tggggaccga cgaagcgttc
      241 tggctctccc gcaccgtgtc ggccccactt ccgacgttcg ggttccaata cgacctcagc
      301 ctggaaccgg ttcgggtcaa tcgcgatcgc atgctgcagg tcttccggaa cggggtccgc
      361 gaattcgcgt cgattctgag tccgatccta tcgccggcga cgctcgaagc gatccagcgt
      421 ctgatcgacg tgagcgacgc ggacctccgc tttccggacg aactctgggt gaaaacgatc
      481 tacgagttcg ccgcctccta ccatcacggc gtgatccatc gcgaccacct gctgcaagcg
      541 ctggtgcccc tctacctcgg acgcacgagt tcgttcgtcg ccgaaaatct gggaaccgat
      601 tcgatggagt tcgaacaacg gctggaggcc ttgtgcctgg aatacgagcg gttcaagccc
      661 tatctcatcg agcgctggaa gcccaccacg tgaggcaccc atgaccgaga ccttgctcaa
      721 tggcgtcaca cagacccttg acgcggccct gaaaatgggc atcgagcttt tgcccaagat
      781 ccttgggatg ttgatcatca tcgcgatcgg ctgggtgatc gcgatcgtcg tcaggctggc
      841 agtccgccgc ctcttgagcc tggtgaagtt caacgcgctg tgcgagaacg tgggagcgac
      901 gcagatcgcg gccaaggccg atctgccccc gccggtcgac ctgctgagcg cgctggtgtt
      961 ctgggtcacg tgggccgcgt tcctgatgtt gggcatcagc acgctgggca tcgtcgtgct
     1021 gcaggaacag atcgccgagt ttttccggta cgtcccccag atcttcattg ccgtggtgat
     1081 cctctttatg ggactcctct tcgccaactt cacggcgcgg gcggtgctgc tggggatggt
     1141 gaacgccaac gtcccatcgg cccggctgtt gagcaacgcc gtccgcgtgt tcctcgtgat
     1201 gttgagcgtg gtgatggccc tcgaacagat cgccttggcc aaaaccgtgg tgttgatcgc
     1261 cttctccatc gccttcggcg ccgtgatgct cggcttggcc atcgcgttcg ggttgggagg
     1321 acgcgacgtc gcccggcgta tgctcgaacg gcagttcacg gaggacgcca agagcgagaa
     1381 agacgaaatc tcacacctct gacgacgcac gcgccccgcg cccgccggtt ccgcgcagga
     1441 tcgcccagcc cacgcccccc gcgaccagcg acgcgcccag gatccccagt ttggcgattg
     1501 gcaagaacgg cgtctccgcg aacgccaggt tcgcgataaa gagcgacatg gtaaacccga
     1561 ttccgcccag ccacgccgcg ccatagacgt ggtgccatcg caggtccggc ggcaacgcgg
     1621 ccaggcccga tcgaaccgcg ccccacgcgc acaggaagat gccgagttgc ttgccgagga
     1681 ccagtccgaa gaacacgccc agaaccacgg ggtccgccag catcgagagc cccagcccgg
     1741 agaacgagac gccggcgttc gcaagcgcga agagcggcat gacgacgaac cgcatcgccg
     1801 gatgcagcgc gtgctccaat cgctccatcg gcgtttgaac ctgaatggcg gcgctcttga
     1861 gcgcctggag cgcggattgg tgcgcctcgg tgatgaactg atcctcgcgg gagtggcctg
     1921 cccgctcaaa ctccgccagc gtcgcgcgac tctgggccag aaactgatcg ctggcaaggc
     1981 gagcgcggga cgggatcgtc atggccaacg ccacgcccgc gatcgtggcg tggatcccgg
     2041 atgagaggac ggcggaccac agtccaaggc ccaggactcc gtacacgagc ggatggcgga
     2101 cgccctgccg gttcagctcc atcagcaccg cgaggataac gagtgccgga aacacgcttc
     2161 cccagtgaat cgtgtccgta taaaagagag cgatcacgac caccgccccc aggtcatcga
     2221 cgatcgccag cgcggtgagg aagaccttga gcgacaacgg gacccgtttg cccagcaggg
     2281 tcaagatccc gagggcaaac gcgatatccg tc
//
LOCUS       DBNGTT010000464         3105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2536817, whole genome shotgun sequence.
ACCESSION   DBNGTT010000464 DBNGTT010000000
VERSION     DBNGTT010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2536817"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1149)
                     /locus_tag="AC16BC_10565"
     CDS             complement(262..1149)
                     /locus_tag="AC16BC_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242103.1"
                     /translation="MNGDPMSVRSGRRVCAWIMGVLLLMTAGCEGTGALAKRPTATAA
                     SAPTGAASPQTPTPLAQPPRTPSPVPPAQSPQTPSPVPPAQSPQTPSPAPLAQSPQTP
                     SPEVSASSPSTNPNAGPGVAFRAPVSAFPSTASVIKGKKPNEIIEIVLPGRMYDPPMK
                     LEPVAARSQADRRTPEQASASDFSAMRSGEPEWIKENYTAEEYSSIQRLVEDPNMRKM
                     NQGVFMRHESKTVVASCLYKEYALVFVQYDNAKATGLLETYAKVGPIWKRTNALSNDE
                     TAAVVLFLFRNGEVAPAPQ"
     gene            complement(1164..1613)
                     /locus_tag="AC16BC_10570"
     CDS             complement(1164..1613)
                     /locus_tag="AC16BC_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242104.1"
                     /translation="MKRLVFAAVLATLTTLAGPVGTAAAADAPVTVTVQVIQASNQGS
                     AVDPALAKIRSQLSSLKFSSYRLLETRPVTTALGAKHTLALPGGRTLDLYPYGISGGS
                     LELLVTIMDGSKRMLDTTVRLPRNGTIVVGGPAHGDGVLIVALSGTF"
     gene            complement(1610..2143)
                     /locus_tag="AC16BC_10575"
     CDS             complement(1610..2143)
                     /locus_tag="AC16BC_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3242105.1"
                     /translation="MSNELTMIAAGDCGALEPWLSAWFDGEVFGEDRARIERHVAGCE
                     RCRAALDGYRLMGSAIRGAARTAATRDLTAITWPPKPAPRPVSAEPALTPRRSLLYDV
                     ARWINPWPVFVGAAAMIALILGIGMWRPSPPDALVEIERLDAAGPVMVLTANGGRTAI
                     IWLSEPEESPPSGPNPI"
     gene            complement(2133..2759)
                     /locus_tag="AC16BC_10580"
     CDS             complement(2133..2759)
                     /locus_tag="AC16BC_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3242106.1"
                     /translation="MPTDLANPMSGTGTQPSEPDDATWVAKARRGDEQAFGELVTRYR
                     TRMYQVAYGMVSHHETAMDLTQEAFLRAYQHLPAFRGDAKFSTWMRRIVTNVCLDHLR
                     KAETKAAPASYDESRAEGDDPEGVSLAGSLPSPDAGVSRRELGRAILNALRELTPEQR
                     AAIVLREVEDMSYEEIAKTLDCAVGTVMSRLHYARKRLQSLLREFHEQ"
     gene            complement(2761..>3105)
                     /locus_tag="AC16BC_10585"
     CDS             complement(2761..>3105)
                     /locus_tag="AC16BC_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242107.1"
                     /translation="GRHGARPTDTAGRWWFVPHLGGAVCPSCGVGALAGSNGQYAGLP
                     MTPGVHALLRQALRMPPSARHRVSAGAELIQEARGLLEACVAERGRRLIKRPVPNQPP
                     ARSVNAATVRRV"
BASE COUNT          491 a         1068 c         1025 g          521 t
ORIGIN      
        1 cccagtcgcc gtagggatcc aggtcccggg tgaatgtcgc atccccggag acggtatcct
       61 cctcagcccg gtcgtcccac tccggatcgt cgtgcgcgga cgccgtgacc ggtccgcaca
      121 gtcccaacgc cgccacacat gccgtgatcc cgatcaatcg cgctcgccac atggctcccc
      181 tcctgacgca gagtcgtctc gcgctagaca gacgtgggaa ccgggggatt attcacgccg
      241 gacaaagggt ccggcgcgga cttattgggg ggccggcgcg acctcgccgt ttcggaagag
      301 gaagagcacg acagcggcgg tttcatcgtt cgacagcgca ttggtgcgct tccagatcgg
      361 accgactttg gcgtacgtct caaggagtcc ggtggccttg gcgttgtcgt attgcacgaa
      421 caccaaggca tactccttgt acaggcacga ggcgaccacg gttttcgatt catgccgcat
      481 gaagacccct tggttcatct tccgcatgtt cggatcttca accaatcgct gtatcgagga
      541 gtactcctcc gcggtgtagt tctccttgat ccattccggc tcacccgagc gcatcgcgga
      601 gaaatccgag gcggacgcct gctcgggcgt gcgtcgatcc gcctggctcc gggccgcgac
      661 cggttcgagt ttcatgggcg ggtcgtacat ccgcccggga agaacgattt cgatgatttc
      721 gttcggtttc ttgcctttga tcacggatgc ggtgctcgga aacgcggaga ccggagcccg
      781 gaacgccaca ccaggtccgg cgttggggtt cgtggacggc gagctcgccg aaacctcggg
      841 actcggggtc tgcgggctct gagccagcgg agccggactc ggcgtctgtg gactctgagc
      901 cggcggaacc ggactcgggg tctgcgggct ctgagccggt ggaaccggac tcggcgttcg
      961 cggcggttga gccagcgggg tcggcgtctg cgggctcgcg gccccggtcg gagcggaggc
     1021 cgcggtcgcg gtagggcgct tggccaacgc gccggtgccc tcacagccag cggtcatgag
     1081 cagcaacacg cccatgatcc acgcacagac tcgccgtccc gatcgcaccg acatagggtc
     1141 cccgttcatc gcgtgatcct tgtttaaaac gttcccgaca gcgcgacgat caatacgccg
     1201 tcaccgtggg ccggtccgcc caccacgatg gttccgttcc gcggaagcct cacggtggtg
     1261 tccagcatcc gtttggatcc gtccatgatc gtcaccaaca attccaggct gccgccggaa
     1321 atcccgtagg gatacaaatc cagcgtgcgt cccccgggca atgcgagggt gtgtttggcg
     1381 cccaacgcgg tggtcacggg gcgggtttca aggagccgat agctgctgaa cttcaacgac
     1441 gacaactggg agcggatttt cgccagcgcg ggatcgaccg ccgagccttg attggaggcc
     1501 tggatgactt ggaccgtcac ggtgaccggg gcgtcggccg ccgcggccgt gccgaccggg
     1561 cccgcaaggg tggtgagggt cgcgagcacg gccgcgaaca cgagacgttt catatcggat
     1621 tcggtccgga tggaggcgac tcttcgggtt ccgacagcca gatgatcgcg gtccgaccgc
     1681 cgttcgcggt caacaccatc accggtcccg ccgcgtccag acgctcgatc tccaccagcg
     1741 cgtccggcgg cgacggccgc cacatgccaa tgcccaggat caacgcgatc atcgcggccg
     1801 ctccgacaaa gaccggccag ggattgatcc agcgcgcgac atcatagagg agcgagcgcc
     1861 ggggtgtcaa ggccggctcc gcggaaacgg gcctcggagc cggcttcggc ggccaggtga
     1921 tcgccgtgag atcccgcgtc gccgcggttc gggcggcccc tcggatcgcg cttcccatga
     1981 gtcgatagcc gtcgagcgcg gcccggcagc gttcgcagcc cgccacgtgg cgttcgatcc
     2041 gcgcgcgatc ctcgccgaac acttcgccgt cgaaccaggc gctcagccac ggctccagcg
     2101 cgccgcagtc tcccgcggcg atcatcgtca actcattgct catggaactc ccgcaacagc
     2161 gactgtaacc gcttcctggc gtaatgcaac cgcgacatga cggtgcccac cgcgcagtcc
     2221 agcgtcttgg cgatctcctc gtacgacatg tcctccactt cgcgcagcac gatcgcggcg
     2281 cgttgttcgg gcgtgagctc ccgcagcgcg ttcaggatcg cccggcccag ttcgcggcgc
     2341 gacacccccg cgtcggggga gggcagcgac cccgccaaac tgacgccctc cggatcgtcg
     2401 ccttcggccc ggctctcgtc gtaggacgcg ggcgcggcct tggtctccgc cttgcgcagg
     2461 tggtccaggc acacgttggt tacgatccgc cgcatccagg tcgagaactt cgcgtcgccg
     2521 cggaacgccg gcaggtgttg atacgcccgc agaaacgctt cctgcgtcag atccatcgcg
     2581 gtttcatggt ggctgaccat cccgtaggcg acctggtaca tgcgggtgcg gtatcgagtc
     2641 accaactccc cgaacgcctg ctcgtccccg cgccgagcct tggcgaccca cgtcgcgtcg
     2701 tcgggctccg acggctgcgt cccggtgccg ctcatcgggt tcgcgagatc cgtcggcacg
     2761 ttagacgcgg cggacggtcg cggcattcac ggatcgggcc gggggctgat tcgggaccgg
     2821 gcgcttgatg agacgccgtc cccgttcggc gacgcaggcc tccagcagcc ccctggcctc
     2881 ctgaatcagt tcggcgccgg ccgatacgcg atgtctggcc gagggcggca tccgaagcgc
     2941 ctggcggagc aaggcgtgaa cgccaggcgt catcgggagc cccgcgtact ggccgttgga
     3001 cccggccagc gcgccgaccc cgcacgaggg gcagaccgcg ccgcccaggt ggggaacgaa
     3061 ccaccaccgc cccgcggtgt cggtcggccg cgcgccgtgg cgccc
//
LOCUS       DBNGTT010000465         5230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2537035, whole genome shotgun sequence.
ACCESSION   DBNGTT010000465 DBNGTT010000000
VERSION     DBNGTT010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2537035"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..1561)
                     /locus_tag="AC16BC_10590"
     CDS             complement(299..1561)
                     /locus_tag="AC16BC_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3242108.1"
                     /translation="MKKSLPHKFVLDNGVRVLMEPMASVRSAAVGVWVDVGSRDEGPG
                     EEGLSHFLEHMFFKGTRKRSAHALAREIDVLGGELNAFTSRENTAFYTKALDEHLPRA
                     VALLGEIFLDSTFVRQELEREKQVVLEEIKMVEDDPEEFIYDLHTDLVWRDHPLGRPI
                     LGEIPVIESVTRKRLTEFLARKYRPQNTVISVAGRIDPSAFEDLIAKTFSRFDGGGPG
                     SARHTPPTTRGGVFLKRKALEQVHICLGAQGLPEGHKDRFALHVLNTVLGGSVSSRLF
                     QEVRERRGLAYSIYSSTASYQDAGLFTIYAAAGKASAQRVINLCLRECRSLRKRPVPK
                     DELAHAKDHLKGSLILGMEGTYSRMSRLAKDELYGGRHVSTTEIIHGIDAVSSDDLLR
                     IANTCLAPETLALTVLGPMQRRDLPATL"
     gene            complement(1623..3731)
                     /gene="pnp"
                     /locus_tag="AC16BC_10595"
     CDS             complement(1623..3731)
                     /gene="pnp"
                     /locus_tag="AC16BC_10595"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3242109.1"
                     /translation="MVERLEMDLAGRRLIIEQGRVARQADGAVLVQLADTVVLVTAVA
                     AKSPKPGVDFLPLTVDYQEKAYAAGKIPGGFFKREGRPAEREILSGRLIDRPLRPLFP
                     KRYYHETQVIASVLSADQSNVADCLGIIGASAALSISSAPFQGPVAGIRIGRVDGNFV
                     VAPSFAEVEKSTLNVVVAGTRDAVMMVEGEASELPEDVMLEAIMLAHEEIKKIIALQD
                     ELVRRVGKPKIEVTKVGPDAALVEAVHRDATNQVRDALLIPNKTERSGRMQQILDGLI
                     ASHGSTDAPEKNRQVMEIFHDLERSEMRLKILDKGVRADGRGPKDIRAITCEIGVLPR
                     THGSALFTRGETQSLSVATLGTSADEQRIDALEGESTKSFMLHYNFPPFSVGEARPVR
                     GPGRREIGHGALAERALKPVIPSKEAFPYTLRLVSDILESNGSSSMATVCGGTLALMD
                     AGVPISAPVGGIAMGLIKEGDRVEILSDILGVEDHLGDMDFKVTGTRKGVTAMQMDIK
                     IAGLSREILEKALAQAKIGRYHMLDKMAEAIAEPRGKLSAHAPRIVTMKVKPDKVREV
                     IGPGGKVIRGIIAKTGVKIDVEDDGSIAIASADEAAAAKAMEMIKQITEDVEVGKLYM
                     GKVTRIMDFGAIVEIGPGVDGLVHISQLAPHRVNNVSDEVKEGEEILVKVLEVDRQGK
                     IRLSRKEAMGAAATTEQTKP"
     gene            complement(4098..4364)
                     /gene="rpsO"
                     /locus_tag="AC16BC_10600"
     CDS             complement(4098..4364)
                     /gene="rpsO"
                     /locus_tag="AC16BC_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3242110.1"
                     /translation="MLEKTVKQGVINTFQRHTTDTGSPEVQVAILSKRITELSDHFKT
                     HAKDHHSRRGLIMMVSQRRKLLDYLKRTNAASYYKLIQQLEIRR"
     gene            complement(4406..>5230)
                     /gene="truB"
                     /locus_tag="AC16BC_10605"
     CDS             complement(4406..>5230)
                     /gene="truB"
                     /locus_tag="AC16BC_10605"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3242111.1"
                     /translation="ICIGRATKVAHYLLETDKDYRVVMRLGATTDTQDGTGRVLTRSA
                     LRPAADEILRVLSGMVGTQTQIPPMYSAIKVDGEPLYKAAREGRVVERRPRTVTIARL
                     EVLGIAEDEVTFDVTCSKGTYIRTLCADAGERLGVGAYMQALERRRVGAFGIDRAMTL
                     TDVEAAVRNGTVGERVLSIDEALIDVPAADVDAATADRVCHGVPLSASRVARWTGACR
                     AGGLVRIRANGATIALGTAPVDHVQLTSRGTTAVIRVDRVLREGASKVAGGHDRAL"
BASE COUNT          877 a         1784 c         1593 g          976 t
ORIGIN      
        1 cgagataccc cgtcgggctt gatcaacgac aacgtcttct caatcatgtg ccatccgctc
       61 ctcggctcaa aaacagcgcg tacaatatcg gaaagacccc ggagtgtcaa cggcggcgat
      121 gtgaccgtgc tcgtaatcga acccgttgcc actcattctc atggagcgct ggcatgcgat
      181 gaccagtgaa tctagacgtg gcgaacatga gccgaatcgg cccgacctgc tgagttcccc
      241 gacaatcagg gcagaccagg agactcccgc catcgcgggc atgcgaggcc aaacccgctt
      301 acagcgttgc cggcagatcc cggcgctgca tcggtcccag cacggtgagt gccagggttt
      361 cgggggcgag gcaggtgttg gcgatgcgga gcaggtcgtc ggaactcacg gcgtcgatgc
      421 cgtggatgat ctcggtcgtg gagacgtggc ggccgccgta gagttcgtcc ttggcgaggc
      481 ggctcatgcg gctgtaggtg ccttccatgc ccaggatcaa gctgcctttg aggtggtcct
      541 tggcgtgcgc gagttcgtcc ttgggcacgg gccgcttgcg caatgaccgg cactcgcgca
      601 gacacaagtt gatcacgcgt tgcgccgacg ccttgcccgc ggccgcgtag atggtgaaca
      661 gccccgcgtc ctggtacgac gcggtggagg agtaaatgga ataggccagg ccacgccgct
      721 cgcggacttc ttggaacagg cgcgagctca cgctgccgcc caacacggtg ttgagcacgt
      781 gcaacgcgaa gcggtccttg tgcccttcgg gcaatccttg cgcgcccagg cagatgtgga
      841 cctgctccag ggccttgcgc ttgaggaaca cgccgccgcg cgtcgtgggc ggggtgtggc
      901 gcgccgaacc agggcctccg ccgtcgaacc gcgagaacgt cttggcgatc agatcctcga
      961 acgcgcttgg gtcgatgcgg cccgccactg aaatcacggt attctgcggg cgatacttgc
     1021 gcgcgagaaa ctcggtgagg cgcttgcgcg tcacggactc gatcaccggg atctcgccca
     1081 ggatcggccg acccagcggg tggtcgcgcc agaccaaatc cgtgtgcagg tcgtagataa
     1141 actcttccgg gtcgtcctcg accattttga tttcttcgag cacgacctgt ttctcgcgct
     1201 ccaactcctg gcgcacgaag gtcgagtcca gaaagatctc cccgagcaag gctacggcgc
     1261 gcgggaggtg ttcatcgagc gccttggtgt agaacgcggt gttttcacgc gaggtgaacg
     1321 cgttgagttc gccgcccagc acgtcgatct cgcgagccaa cgcgtgggcc gaacgctttc
     1381 gcgtcccttt gaagaacatg tgttcgagaa aatgtgagag gccttcttcg cccggacctt
     1441 cgtcgcgaga gcctacgtcc acccacacgc ccaccgcggc ggagcggacc gaggccatcg
     1501 gctccatcaa cacgcgaacg ccgttgtcga ggacgaactt atggggcagg gattttttca
     1561 tcgacgcggg aggccgatcg cccggtgggg cggccgtgac cggccgcccc accggactgg
     1621 tattacggtt tggtctgttc cgtggtggct gcggcgccca tcgcttcttt gcgcgacagg
     1681 cggatcttgc cctggcggtc gacttccaac accttgacca ggatctcctc gccttccttg
     1741 acctcgtcgc tcacgttatt gacgcggtgc ggcgccaact gcgagatgtg cacaagcccg
     1801 tccacgccgg ggccgatctc cacgatcgcg ccgaagtcca tgatgcgcgt gaccttgccc
     1861 atgtagagct tcccgacctc gacatcctcg gtgatctgct tgatcatctc catggccttg
     1921 gccgcggcgg cttcgtcggc cgacgcaatg gcgatggaac cgtcgtcttc cacgtcgatc
     1981 ttcacgccgg tcttggcgat gattccgcgg atgaccttgc cgcccgggcc gatcacttcg
     2041 cggaccttgt ccggcttgac cttcatggtc acaatgcgcg gcgcgtgcgc ggagagcttg
     2101 ccgcgcggct ccgcgatggc ctcggccatc ttgtccagca tgtggtaccg gccgatcttg
     2161 gcctgggcca acgccttctc cagaatctcg cggctcaagc ccgcgatctt gatgtccatc
     2221 tgcatcgcgg tcacgccctt gcgggtgccg gtgaccttaa aatccatgtc gcccaggtgg
     2281 tcttccacgc ccaggatgtc cgagaggatc tcgacccgat cgccttcctt gatcagcccc
     2341 atggcgatgc cgccgaccgg cgcgctgatc ggcacccccg cgtccatcaa cgcgagcgtg
     2401 cccccgcaca ccgtggccat ggaggacgag ccgttggatt ccaggatgtc cgagaccagc
     2461 cgcagcgtgt agggaaacgc ttccttggac ggaatcacgg gtttgagcgc gcgctcggcc
     2521 aacgcgccgt gcccgatctc ccggcgcccc ggcccacgca ccggccgcgc ctctcccacg
     2581 gagaacggcg ggaagttgta gtgcagcatg aacgacttgg tggactcgcc ttccaatgcg
     2641 tcaatgcgtt gttcgtccgc cgacgtgccc aaggtggcca ccgacaggct ctgcgtctcc
     2701 ccgcgcgtga acaacgcgga gccgtgcgtg cggggcaaca ccccgatctc gcacgtgatc
     2761 gcccgaatgt ccttggggcc gcggccgtcc gcgcgcacgc ccttgtccag gatcttcagc
     2821 cgcatctcgc tgcgttccag gtcgtggaaa atttccatga cctggcgatt cttctccggc
     2881 gcgtccgtgg agccgtgcga cgcgatcaac ccgtccagga tctgctgcat ccggcccgag
     2941 cgctcggtct tgttgggaat caacagcgcg tctcggactt ggttggtggc atcgcgatgc
     3001 accgcctcca ccaacgcggc gtcagggccg accttggtga cctcaatctt gggtttgccc
     3061 acgcgccgga ccaactcatc ctgcagcgcg atgatcttct tgatctcctc gtgcgcgagc
     3121 atgatggctt cgagcatgac gtcttcgggc aattccgacg cctcgccctc gaccatcatg
     3181 accgcgtcgc gcgtgcccgc caccaccacg ttgagcgtgc tcttctccac ctcggcgaac
     3241 gagggcgcca ccacgaagtt gccgtccacg cggccgatgc gaatgccggc caccggccct
     3301 tgaaacggcg cggacgaaat cgacaacgcc gcggacgcgc cgatgatgcc gagacagtcc
     3361 gccacgttgc tctgatccgc ggacaatacc gatgcgatga cctgggtctc gtggtaatag
     3421 cgcttcggga acaacggccg caacgggcga tcgatcaacc ggccggagag gatttcgcgt
     3481 tcggccggac gtccttcgcg cttgaaaaaa ccgcccggga tcttccccgc cgcgtacgcc
     3541 ttttcctggt agtccacggt caacgggaga aagtcgactc ccggcttggg ggacttggcc
     3601 gcgaccgcgg tgaccagcac cacggtatcg gccagttgca ccagcacggc gccgtcggcc
     3661 tgcctggcca cccggccctg ttcgatgatc agccggcgtc cggccaaatc catttctaaa
     3721 cgttcaacca tactgtctcc ttgtgcgcct cctcatgaag gcgcggtgat ggagccccga
     3781 acgagtcaca gtcaatgacg tctgccgacc ccctcacggc gatcgccaaa ggtcattcac
     3841 tgtgcctggc ccggaacccg aattgacgca gggcctacgg agacctgcgc ccgctaacgc
     3901 gaaggtattt ccaatccgac gtaagatcaa gccggttcag gaatgcgtca gatgccccct
     3961 gcggggactt ccgccatttg aattcctggc ggtaggtcgc aaggcgccgc gagcaccgca
     4021 ccggagcgta cgtagcaagt acgtgaggag cggatgcgca gcggcaacgc agcagatgac
     4081 cattcatgga ccggcgacta acgacgaatt tccaactgtt ggatcagctt gtagtagctc
     4141 gccgcgttgg ttcgcttcaa gtaatcgagc agcttgcgcc gttggctcac catcatgatc
     4201 aacccgcgtc gcgagtgatg atccttggcg tgcgtcttga agtgatcgct cagctccgtg
     4261 atccgtttgc tcagaatcgc cacctggact tccggcgacc cggtgtccgt ggtgtggcgc
     4321 tgaaacgtgt tgatcacgcc ctgcttgacc gtcttttcca acatggtgcg tctccttgcc
     4381 tttccgttct ctaccgtgtg atccgttaca gcgcgcggtc gtgtccgccc gctaccttcg
     4441 atgccccttc ccgcaacacc cgatccaccc tgatcaccgc cgttgttcca cgcgacgtga
     4501 gttgcacgtg atcgaccgga gccgttccca acgcgatggt cgcgccgttg gcgcggatgc
     4561 gcacgagccc gcccgcgcgg cacgcgcccg tccagcgcgc cacccgcgac gcggacagcg
     4621 gcacgccatg gcacacgcga tccgcggtgg cggcatccac gtccgcggcc ggcacgtcga
     4681 tcaacgcctc gtcgatggac agcacgcgtt caccgacggt tccgttgcgc accgcggcct
     4741 caacgtcggt cagcgtcatg gcacggtcga tcccgaacgc gccgacccgc cgtcgctcca
     4801 atgcctgcat gtacgcgccg acaccgagtc gctcgcccgc gtccgcgcac aacgttcgaa
     4861 tgtacgtgcc cttggagcag gtcacgtcga acgtgacctc gtcctccgcg atgcccagca
     4921 cctccagccg cgcgatcgtc acggtgcggg gccggcgctc caccacgcgc ccctcccgcg
     4981 cggccttgta caacggctcg ccgtccacct tgatcgcgga atacatcggc gggatctgag
     5041 tctgggtccc gaccatgccg ctcagcaccc gcaggatctc gtccgcggcc ggccggagag
     5101 cggaacgcgt cagcacccgg ccggtcccgt cctgcgtgtc ggtggtggca cccaagcgca
     5161 tcaccacccg atagtccttg tccgtttcca gcaagtagtg cgcgaccttg gtcgcccggc
     5221 cgatgcagat
//
LOCUS       DBNGTT010000466         5322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_878311, whole genome shotgun sequence.
ACCESSION   DBNGTT010000466 DBNGTT010000000
VERSION     DBNGTT010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_878311"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..586)
                     /locus_tag="AC16BC_10610"
     CDS             complement(362..586)
                     /locus_tag="AC16BC_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242112.1"
                     /translation="MRAFVRLRQILASHAELAQQLAQLEQKYDAQFRVVFDAIRELMA
                     PPKTERRRIGFEVRERRPRYGRQAAGERAV"
     gene            complement(667..816)
                     /locus_tag="AC16BC_10615"
                     /pseudo
     CDS             complement(667..816)
                     /locus_tag="AC16BC_10615"
                     /inference="COORDINATES: protein motif:HMM:NF022018.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(938..1288)
                     /locus_tag="AC16BC_10620"
     CDS             complement(938..1288)
                     /locus_tag="AC16BC_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3242113.1"
                     /translation="MKKETLRERLARYPTLDESIKAREQRDPEFQAELARARLRVAIA
                     RAIKHARELAGMTQAELAKALDLKQSAVGRLESLRDQRLPSVDLLLRISAATRKRLVV
                     KQAGMRIELVAKTA"
     gene            complement(1285..1473)
                     /locus_tag="AC16BC_10625"
     CDS             complement(1285..1473)
                     /locus_tag="AC16BC_10625"
                     /inference="COORDINATES: protein motif:HMM:NF017757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3242114.1"
                     /translation="MRQIRGKLWELRISRTRIFYVVVVGDTMVLLHAYKKQGQKAPTR
                     EIETATRRMAEVLEGRRT"
     gene            complement(1614..1886)
                     /locus_tag="AC16BC_10630"
     CDS             complement(1614..1886)
                     /locus_tag="AC16BC_10630"
                     /inference="COORDINATES: protein motif:HMM:NF022018.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HIR3242115.1"
                     /translation="MGYTSSSVRTENIESRILVVRGQKVMLDADLAELYGVTTKRLNE
                     QVNRNRRRFPGDFSFHSRPRRRPRWSHIATTSRTRSLGPRLRKNNQ"
     gene            complement(1954..4587)
                     /locus_tag="AC16BC_10635"
     CDS             complement(1954..4587)
                     /locus_tag="AC16BC_10635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3242116.1"
                     /translation="MELTLTDSGTLWARVQGTEPRPYLVTVAPVGPTGLLWECTCPYF
                     RDRTDEACKHVIAVLLAWVSRRDRAPRRDSAPVLRRPRQVAPWTPTWAPTRFLPPRQE
                     PALPSVLDSATWTELRLDVIESAGGPPRVEVRFKPHGDEDSALLTLPPGATPDALMAL
                     RGRATYSPTALNIRLLRTPLRAELHASYDAAGRLVLTPSFRHPSRAGRNPGPRIVIVN
                     PSWIWSDGVFFRTEVVPDAFRPYFSHAEPTVLEGDAIHRFLEHDAPRLSEYPRYRPSH
                     DVRQSRLLPAPRFNAVSVFGKDRDWFWLDPLYQAGEHRFTLSELLDAAERNKPVRRGN
                     DWIAPPTAVSEAWEASGGRLDAGRVVMPRLGYLRRRADWGDLDVERDATVRRFEADLD
                     RLTPARPAPEPPDYKGSLRPYQRTGYDWLWFLHVNGFHGVLADEMGLGKTHMTMALLL
                     AAGREDAVRPSLIVCPTSVIDHWEDKLHEYAPSLAPLRYHGMARTGIATGALPPVVLT
                     SYAILARDSERFRAIEWNYVVLDEAQKIKNPATQSAKAAKALAARHRLALTGTPIENR
                     LAELWSIFDFLLPGYLGTARSFRREYELPIAKHGDREREAVLKRVIHPFKLRRLKAEV
                     LKELPPKVEDLRHCRLSPAQAAIYRDLVAHEGQPLADEIRQGSQPVPYIHVFALLSRL
                     KQLCDHPALLIKGAKGRKLASGKFDLFTELMDEALDAGEKVVVFTQYLGMIDLIGAWL
                     DQQHVDWVELRGATRDRRAVVKRFQTDPQCRVFVSSLLAGGLGIDLTAASIVIHYDRW
                     WNAARENQATDRVHRIGQSRGVQVFKLITRGTLEDKIDKMIREKASLLDAIVEEDAAT
                     LKALTREELVELLTSVPAESE"
     gene            5090..>5322
                     /locus_tag="AC16BC_10640"
     CDS             5090..>5322
                     /locus_tag="AC16BC_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HIR3242117.1"
                     /translation="MGLTTARLLAAGFSVTAQGAAASGKSGAFTAQADDPSALYYNPA
                     GITQLKTTELLVGTSIIVPRQAYHPDLGGPRWD"
BASE COUNT          965 a         1668 c         1682 g         1007 t
ORIGIN      
        1 cgtgcttata ctccgccgcg tccatgttgt tgcgcaggcc gtcggccatc tgccacaact
       61 tggcttcgaa accgaggttg ccgccgttac tggccttctt cggttgtttt gatttcgcag
      121 ccattgaccc tcagtgcaag taagtgacgc aaccgcgagc gcgttgcggg attggaacgg
      181 ctcgcaacga gtgccgggcc tcggatattt actaaggtgg aggcccagct tagcccgttg
      241 cgggggcgag ggtcaagaag gggaaaagga cgagggggtg aggagcaaag cgacgagccg
      301 gggaatcggt gaatcggggt gtcggagaaa cggcgtactg aaaaagaacc gacaaagcgc
      361 gtcacacggc tcgctcccct gccgcctgcc ggccgtagcg cgggcgtcgc tcgcggacct
      421 cgaagccgat tcgccggcgc tcggtcttgg gcggggccat gagttcccgg atggcgtcga
      481 agacgacccg aaactgcgcg tcgtatttct gctcaagctg cgcgagctgc tgagccagtt
      541 ccgcgtgcga ggccaggatc tggcgcagcc gcacgaaggc gcgcatgatc tcgatgttga
      601 gttgagcacg gtggagagca tcgcgatgcc ctgctccgtg aacgcgtacg gagcgtattt
      661 tcgatgccgg ccacgcccga gctttaaggt cgcaaaatgc gaccttaaag ccgcgaactc
      721 ttctccggtc agttggaaca tgaagtcaaa tgtgctgcaa aaacgggaca ggtctgtttt
      781 ctcccacgcg gcttcgaggg gcgcaagcat ttccattttg gaaacggttc gtcggcactg
      841 gcgtgtccgc cgcgcaattg tcccgccgcc tcactactac accgcccggc gcaccttcat
      901 cgcaacatcc cgtatcttcg gtcgatcgtc ctcaacgcta cgcggtcttg gcgactaatt
      961 cgatccgcat ccccgcctgc ttcaccacca atcgcttgcg ggtggccgcc gagatacgaa
     1021 gcagcaggtc gacgctgggg aggcgttgat cccgcaggct ttccaaccgg ccaaccgcgg
     1081 actgtttgag atcaagcgcc ttcgccaatt ccgcctgcgt catcccggcc aattcgcgcg
     1141 cgtgtttgat ggcgcgggca atggcgaccc gaagtctggc gcgggccaat tcggcctgaa
     1201 actccgggtc ccgctgttcc cgtgctttga tcgactcgtc cagggtggga taacgagcca
     1261 gccgttcacg cagcgtctcc ttcttcatgt ccttctcccc tccagtactt cggccatgcg
     1321 acgagttgcg gtctcgatct cgcgcgtcgg cgccttttgt ccctgcttct tgtaggcgtg
     1381 caacaagacc atcgtgtcac caaccaccac cacgtaaaag atccgggtcc gcgagattcg
     1441 cagctcccat aacttgccac ggatctgccg catcgagccc gtatcggcgg aggtcttcga
     1501 ggacggcgag cacttctgca cgctccgcga cgggaagttc atcaagatac ccatcgacgg
     1561 gactgcgccc actcgccgtg gtgtaccgtc gaatctgcac gactcaatat aacctattgg
     1621 ttattctttc gcaacctagg cccgagactt ctggttctgg aggtggtcgc aatgtgcgac
     1681 cacctcggtc ttctcctggg acgagagtga aaactgaaat cgccggggaa ccgacggcgg
     1741 ttacggttga cctgctcgtt gaggcgcttg gtggttactc cgtacagttc cgccaggtcc
     1801 gcatccagca tgaccttctg gccgcgaaca acgagaatcc ggctctcgat gttctcggtc
     1861 cgcacgctcg acgatgtgta ccccatacgg tcctccttaa taacgatggc tgaccctccg
     1921 gtgggtcacg cggctcggcg ggccgtcctc tggctactcg ctctccgccg gcacgctggt
     1981 caacagctcg accaattcct cgcgcgtcaa cgccttgagc gtggccgcgt cttcttccac
     2041 gatggcgtcc aggagcgagg ctttctcgcg gatcattttg tcgatcttgt cctccagggt
     2101 tccgcgcgtg atcagtttga agacctggac gccgcgggat tggccgatgc ggtggacgcg
     2161 gtcggtggct tggttttcgc gggccgcatt ccaccagcga tcgtaatgga tcacgatcga
     2221 ggcggcggtg aggtcgattc ccagcccgcc ggccaacagg ctggagacga agacgcggca
     2281 ctgggggtcg gtctgaaagc gcttgaccac ggcgcggcga tcgcgcgtgg cgccgcgcag
     2341 ctccacccag tccacgtgct gttgatcgag ccatgcgccg atcaggtcga tcatgcccag
     2401 gtactgggtg aagaccacca ccttctcgcc cgcgtccagg gcttcgtcca tcagctccgt
     2461 gaacaggtcg aacttgccgg acgccagctt tcttcctttg gcgcctttga tcagcaaggc
     2521 ggggtggtcg cagagttgtt tgaggcgcga gagcaaggcg aagacgtgga tatacgggac
     2581 cggttgggac ccttgtcgga tctcgtccgc gagcggctgc ccctcgtgcg ccaccaggtc
     2641 gcggtagatg gcggcctgcg ccggcgagag gcggcaatgg cggaggtctt cgaccttggg
     2701 gggcagttcc ttcaagacct cggccttgag tcgccgcagc ttgaacggat ggatcacgcg
     2761 cttgagcacg gcttcccgct cgcggtcccc gtgtttggcg atcggcagtt catactcccg
     2821 acgaaacgac ctggccgtgc ccagatagcc gggcaggaga aagtcgaaga tcgaccagag
     2881 ctcggccaga cggttttcga tcggggtccc ggtcaatgcc aggcggtgac gcgcggccaa
     2941 ggccttggcg gccttggccg attgggtcgc ggggttcttg atcttctgcg cttcgtccag
     3001 caccacgtag ttccactcga tggcgcgaaa ccgctcgctg tcgcgcgcca agatggcata
     3061 gctggtgagc acgaccggcg gcagcgcgcc cgtcgcgatg cccgtccggg ccatgccgtg
     3121 atagcgcaac ggggccaagg acggcgcgta ctcgtgcagc ttgtcctccc agtgatcgat
     3181 cacggacgtc gggcacacga tcagggacgg ccgcaccgcg tcctcgcggc ccgcggcgag
     3241 cagcaacgcc atggtcatgt gcgtcttgcc cagccccatc tcgtcggcca acacgccgtg
     3301 gaatccattg acgtgcagga accagagcca gtcgtacccg gttcgctgat acgggcgcag
     3361 cgagccttta tagtccggag gctccggagc cggacgcgcc ggggtcaggc gatcaaggtc
     3421 ggcttcgaat cgccgaacgg tcgcgtcgcg ttcaacgtcc agatcgcccc aatccgcccg
     3481 ccgcctgaga tagccgagac gtggcatcac cacgcgcccg gcgtccaggc gcccgcccga
     3541 agcttcccaa gcctccgaca cggccgttgg cggcgcgatc caatcgttcc cgcgtcggac
     3601 cggcttgttc cgctcagccg cgtccaggag ctcgctcaag gtgaagcggt gttcgcccgc
     3661 ttgatacagg ggatccagcc agaaccagtc gcggtccttg ccgaatacgg acaccgcgtt
     3721 gaatctcggg gcgggcagca aacggctctg acggacatcg tgcgacgggc gatagcgagg
     3781 atattccgac aaccgcggcg cgtcgtgctc aaggaaccgg tgtatcgcgt cgccttccag
     3841 gaccgtgggt tcggcgtggg agaagtacgg tcgaaacgcg tccgggacga cctcggttcg
     3901 gaaaaagact ccgtcggacc agatccacga cgggtttacg atcacgatcc gtgggcccgg
     3961 attccgtccc gcgcgggagg gatgacgaaa cgacggggtg agcaccaacc ggccggccgc
     4021 gtcgtacgag gcgtgcagct ccgcgcgcaa cggcgttcgc agcaaccgga tgttgagcgc
     4081 cgtcggcgaa taggtcgcgc ggccgcgaag cgccatcaac gcgtcggggg tcgcgcctgg
     4141 gggaagcgtg aggagggcgc tatcctcgtc gccgtgcggc ttgaaccgta cttcgacccg
     4201 cggcgggccc cccgcgctct cgatcacatc gagccggagc tccgtccatg tcgcgctgtc
     4261 gagaacggac gggagcgccg gctcctgccg cggcgggaga aaccgcgtgg gagcccacgt
     4321 cggagtccag ggggcgactt gccgcgggcg tcggagcacg ggcgcggagt cgcgtctcgg
     4381 cgcgcggtct cggcgggaca cccaggccag cagcacggcg atcacgtgtt tgcatgcttc
     4441 gtcggtgcga tcgcgaaaat acgggcaggt gcactcccac agcagcccgg tcggtcccac
     4501 cggcgcaacc gtgacgagat acggtctggg ctcggtgcct tgcacgcgtg cccagagtgt
     4561 gccgctgtca gtcagagtca gctccacgac cgcgccctgg cgataatact catttccacg
     4621 ctcgaatacc gggggcgagg ccaaagcacg gatgtggggt agggtcaggc ttacgagaga
     4681 aaccgacata acgacatatg ataagtcgga ttagccagtc gcgacaagga gctatgtgat
     4741 cattttttga cgtgaagcga atcgagcatt tcgataaccg cggtattaca tagagtggat
     4801 ctccatgaaa ctaaatccgg tccaatagaa tacttctcgg gatatgccca ggttagatat
     4861 ttcgattgca ctggtgcgca ggacgacata taattttttt acccaatatt gtccgaagag
     4921 cccctgttga aggaggtgat aagtgccgcc cacacctgca gaacacctgg taacggagtg
     4981 ccgacgggac acatgtggcg ggcggactct ttgccgccga cttggtcggc ccgatgtcct
     5041 aattcgcagg gcgggttccc tcctaataat cttcatcatc ctcgactcgg tggggctcac
     5101 aacggcgagg ctacttgcgg cggggttttc tgttaccgca caaggcgcag cagcaagcgg
     5161 caagagtggc gcgtttacgg cacaagccga tgatccctct gcgctgtact acaatcctgc
     5221 tggcatcact caactgaaaa cgacagagct cctcgtcggt acctccatca ttgttccgcg
     5281 ccaggcatat cacccagacc tcggcggccc tcggtgggac ca
//
LOCUS       DBNGTT010000467         4906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1516616, whole genome shotgun sequence.
ACCESSION   DBNGTT010000467 DBNGTT010000000
VERSION     DBNGTT010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1516616"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..336
                     /locus_tag="AC16BC_10645"
     tRNA            260..336
                     /locus_tag="AC16BC_10645"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:294..296,aa:Met,seq:cat)
     gene            complement(448..1833)
                     /locus_tag="AC16BC_10650"
     CDS             complement(448..1833)
                     /locus_tag="AC16BC_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3242118.1"
                     /translation="MSVTVRNPYDQRVVARLPLDQGKTLERKIENAAAAYTVWRRVPL
                     DERIQRVNQGLAYFQDHAEEIAREITLQMGKPIAEARGEVKTFFARAEYMLSIAKDTL
                     APEIVPGKPGFHLRIEQAPLGVVYNIAPWNYPLLTAVNVVVPALLAGNAVLLKHSPLT
                     PLVGQHFERAFAHGNPPNLVTKLILTNAQASRLLLDPRINYVAFTGSVATGTAVYRRA
                     AGRLLDVGLELGGKDPAYVAEDADLDFAVANIVDGACYNAGQSCCAVERVYVHRALYR
                     AFLDRAHAVLSKYRLGDPLDEQTTMGPLARCVALPFLEAQVREAVRRGAKLLLGGKRV
                     TGTRGNFFAPTLLADVPNRAKVMQDESFGPIVPVAPVADDHEALARMNDSQFGLTASI
                     WTRDRERVERMACDLEVGTVFQNRCDFLEPSLPWTGARDSGIGSTLSRYGFYHLTRRK
                     AMHFRTGEQTP"
     gene            complement(2037..2810)
                     /locus_tag="AC16BC_10655"
     CDS             complement(2037..2810)
                     /locus_tag="AC16BC_10655"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HIR3242119.1"
                     /translation="MRLKDKVALITGAGNGIGRESALLFASEGAQVAAADVDEAAAKD
                     TVAQIQKAGGEAVAVRADVSQAGECERMVAAAEQRFGRLQILFNNAGIMDGNDDNAMT
                     TDEGVWEKTMAINLKGVFLGCKYGIPALRRAGGGSIINTASFVAMLGAATPQLAYTAS
                     KGGVLAMTRELAVIHAREGIRVNALCPGPLRTELLMKFLNTEAKRQRRLVHIPMGRFG
                     EAKEIAKAALFLASDDSSFMTGASLMVDGGITAAYVTPE"
     gene            complement(2810..3541)
                     /locus_tag="AC16BC_10660"
     CDS             complement(2810..3541)
                     /locus_tag="AC16BC_10660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019495400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3242120.1"
                     /translation="MSLPLIGLTTYGRGEANRFSLPAPYVDAVRRAGGLPILLPPGET
                     HADELLERLDGLILTGGGDLDPAWYRGESHPTIYMVDSERDRHELALVAKIRASGLPT
                     FGICRGSQVINVALGGTLIEHLPDEVGESIAHRLPPREPTTHAVSLTPGSRLAEMLPA
                     REFSAPSWHHQAVRRLAPGLRAAAQATDGTIEAIEFPEHPWLIGVQWHPELAADKDPV
                     HQKLFDELVKAAATRLRKRSARRRT"
     gene            complement(3549..>4906)
                     /locus_tag="AC16BC_10665"
     CDS             complement(3549..>4906)
                     /locus_tag="AC16BC_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR3242121.1"
                     /translation="TRDELSRRVRAGQIDTVLLVFTDHYGRFMGKRLDAGFFLDDAAK
                     RGTHACDYLLTVDMEMEPVPGYRFSNWEKGYGDFHLVPDFATLRVASWLDKTALVLCD
                     VRDERTHQPVAQAPRSILRRQIDRAAAMGYRAMTGSELEYYIFNNSYRDAVGKGYRGL
                     EPAGWYLEDYHALQGGREEGFNGAARRHLRQSGIPVESSKGEWGLGQHELNVRYADIL
                     TMADRHAVFKQCLREIADQMGISVTFMAKFAANQAGSSCHIHMSLWRKNRNAFAGPKR
                     LGPVACSDTFRWFLGGWITHAPEMMVFYAPTVNSYKRYQAGSWAPTRLAWSYDNRTAG
                     FRVVGSGPSLRIECRIPGADCNPYLAFAAALASGLDGIEQRIEPPPIFEGDVYAAQHL
                     PRVPRTLRDATDLFEQSGMAKRALGEDVVEHYLHFYRTEQDAYDKAVTDWERQRYFER
                     I"
BASE COUNT          830 a         1656 c         1510 g          910 t
ORIGIN      
        1 atgtcctcga tcagcgcctg cagcctgccg tccacgttct tgacgggctc cgccgtgcgc
       61 ttgagcacgt cttccgggta ttcgcgaatg cgtaggatcg acatcgcggg tatgatgcca
      121 cgccaaacgc ccgaaatcaa gccaacgccg gcggcacgta gccccaatcg gtatacgctt
      181 ccgccacgat ttacaccgcc cgccgcgcgg ccaaacgcgc cgcttgacgt tggctcaagc
      241 gttttgttac gatgccgtcc gcggggtgga gcagcctggc agctcgtcgg gctcataacc
      301 cgaaggtcgt cggttcaaat ccggcccccg caaccaactt tccggaccat cccgatgctg
      361 acaggccgcg agttcgcggc ctgtggtgtt ttacgggtcg gcggagtctc ggaaccactc
      421 tgcgaactcg ccgttcactt ctttatgtta cggggtctgc tcccccgtcc taaagtgcat
      481 ggccttgcgc cgagtcaggt ggtaaaaacc gtaccgagac aaggtggagc cgatcccgct
      541 gtcacgggcg ccggtccagg gcagcgacgg ttcgaggaag tcgcagcggt tctggaacac
      601 ggtgcccacc tcaagatcac acgccatgcg ttcgacgcgt tcacggtcgc gcgtccagat
      661 ggaggcggtc aggccgaatt ggctgtcgtt catccgggca agcgcttcgt ggtcatccgc
      721 gaccggcgcc accggcacga tcggaccgaa gctttcatcc tgcatcacct tggcgcggtt
      781 gggcacgtcg gccagcaggg ttggcgcgaa gaaatttccg cgcgtaccag tgacccgctt
      841 gccgcccaac agcagtttgg cgccgcgtcg gaccgcttct cgcacctggg cttccaggaa
      901 cggcaacgcg acgcatcgcg ccagcggccc catcgtcgtc tgttcgtcga gcggatcgcc
      961 cagccggtac tttgagagga cggcgtgcgc gcgatccagg aacgcgcgat acaacgcgcg
     1021 gtgcacgtag acccgttcca ccgcgcagca ggactggccc gcgttgtagc acgcgccgtc
     1081 cacgatgttc gccacggcaa agtccaggtc cgcgtcctcc gccacgtatg ccgggtcctt
     1141 gcccccgagt tccagcccca cgtccagcag acggccggcg gcgcggcggt agaccgcggt
     1201 gccggtggcc actgatccgg tgaacgccac gtagttgatc cgcgggtcca agagaagacg
     1261 cgacgcctgc gcgttggtca gaatcaactt cgtcaccaga ttgggcgggt tgccgtgagc
     1321 gaacgcacgc tcgaagtgct ggcccaccaa cggggtgagc gggctgtgtt tgagcaggac
     1381 cgcgttgccc gcgagcaatg ccgggaccac cacgttcacc gcggtcagca agggatagtt
     1441 ccagggcgcg atgttataga ccacgcccag cggcgcctgt tcgatccgca gatgaaaccc
     1501 gggcttgcct gggacgatct cgggagcgag cgtgtccttg gcgatcgaca acatgtattc
     1561 ggcgcgcgca aaaaaggttt tcacctcgcc gcgcgcctcg gcaatgggtt tgcccatttg
     1621 cagggtgatc tcgcgcgcaa tttcctcggc atggtcttga aagtaggcga gcccttgatt
     1681 gactcgttgg atgcgctcgt caagcggcac gcggcgccag accgtatacg ccgcggccgc
     1741 gttctcgatt tttcgctcaa gggtcttgcc ctgatccagg ggcagcctgg ccaccacgcg
     1801 ttgatcatag ggattgcgaa cggtcacaga cacgcgtttc ctccgttagc aggttgctga
     1861 aaaagtccat ccgctgcgtt ctcgctcgtc ggaactcctc acgtacgacc cagtacgctc
     1921 cgggttcctc ctccctgcgg ccttgcggct ggagcttttt gagcaacctg cgagtttttc
     1981 agtcttcggc cttaccacac actaccgaac aatcgtctga tcactgtgtg atccggttat
     2041 tccggggtca cgtatgcggc ggtgatgccg ccgtccacca tcaatgacgc gccggtcatg
     2101 aaggacgagt cgtcgcttgc caggaacaat gcggccttgg cgatctcctt ggcctcgccg
     2161 aagcgcccca tcgggatgtg caccaaccga cgctggcgct tggcttcggt attgagaaac
     2221 ttcatcaaca gttcggtccg gagcgggccc gggcagagcg cgttgacccg aattccctcg
     2281 cgcgcgtgga tcacggccag ctcacgggtc atggccaaca ccccgccctt gctcgcggta
     2341 taggccaact gcggggtcgc ggcccccagc atggccacga acgacgcggt gttgatgatg
     2401 ctgcccccgc cggcccgacg cagcgcgggg atgccgtact tgcagccgag gaacacacct
     2461 ttgagattga tcgccatagt tttttcccag acgccctcat ccgtggtcat ggcgttgtcg
     2521 tcgttgccgt ccatgatccc ggcgttgttg aacaggatct ggagcctgcc gaatcgctgt
     2581 tccgccgcgg ccaccatgcg ctcacactcg ccggcctggg acacgtccgc gcgcaccgcg
     2641 accgcctccc cgccagcttt ttgaatctgc gcgaccgtgt ccttggccgc ggcttcgtcc
     2701 acgtcggcag ccgccacttg cgcgccttcg ctcgcaaaca ggagcgccga ctcccgtcca
     2761 atcccgttcc cggccccggt gatcagggcg accttgtctt tcaatcgcat catgttctcc
     2821 ttcgcgcgga tcgttttcgc agccgcgttg ccgcggcctt caccagttca tcgaacaatt
     2881 tttgatgcac cgggtctttg tccgcggcca actccgggtg ccactgcacg ccgatcaacc
     2941 acgggtgctc cgggaattcg atcgcttcga tggtcccgtc ggtcgcctgg gccgctgcgc
     3001 gtaacccggg cgcgagccgg cgcacggcct gatggtgcca ggacggggcc gagaattcgc
     3061 gcgcaggcaa catctcggcg agacggctcc cgggcgtcag cgacaccgca tgcgtggtgg
     3121 gctcgcgcgg cggcaaccgg tgcgcgatcg actccccgac ctcatccggc aggtgctcga
     3181 tcaaggttcc gcccaatgcc acgttgatga cctggctgcc gcggcaaatg ccgaatgtgg
     3241 gcaggccgga cgcgcgaatt ttcgcgacca gggcgagttc gtggcgatca cgctccgaat
     3301 cgaccatata gatcgtgggg tgcgactcgc cgcggtacca cgcgggatca aggtcgccgc
     3361 cgccggtcag gatcaatccg tcgaggcgct cgagcaattc atccgcgtgc gtttcgccgg
     3421 gcggaaggag aatcggcagc ccgccggcgc ggcgcaccgc gtccacatac ggcgcgggca
     3481 gcgagaaccg gttggcctcg ccgcggccgt aggtagtcag accgatcagg ggaagggaca
     3541 tgccggggtc agatcctttc gaagtagcgt tggcgttccc agtcggtcac cgccttgtcg
     3601 taggcgtcct gctccgtgcg atagaaatgc aggtagtgtt ccaccacgtc ttcgcccaac
     3661 gcgcgtttgg ccatgccgct ctgctcgaac aggtccgtcg catcgcgcaa ggtgcgcggc
     3721 acgcgcggga ggtgctgcgc cgcgtacacg tcgccctcga agatcggggg cggctcgatc
     3781 cgctgctcga tcccgtccag gccggacgcg agcgcggccg cgaacgccag gtacgggttg
     3841 cagtccgcgc ccgggatccg gcactcgatg cgcagactcg ggcccgaacc gaccacacga
     3901 aaccccgcgg tgcgattgtc gtagctccag gccagccgcg tgggcgccca cgacccggcc
     3961 tggtaacgct tgtacgaatt cacggtgggc gcgtagaaga ccatcatctc gggcgcgtgt
     4021 gtaatccacc cgcccaggaa ccagcgaaac gtgtcggagc acgcgaccgg tcccaggcgc
     4081 ttgggcccgg caaacgcatt ccggtttttc cgccacaggc tcatgtggat atggcagctc
     4141 gacccggcct gattggccgc gaacttggcc atgaacgtga cgctgatccc catttgatcc
     4201 gcgatctccc ttaaacactg tttgaagacc gcgtgccggt cggccatggt caggatgtcc
     4261 gcgtaccgga cattgagttc gtgttgcccc aacccccact cgcccttgga gctttccacc
     4321 ggaatcccgg attgccggag gtggcgtctg gccgcgccgt tgaatccttc ttcacggccg
     4381 ccctgcagcg cgtgataatc ctccaagtac caccccgcgg gttccagacc gcgatatccc
     4441 tttcccaccg cgtcgcgata cgaattgttg aagatgtaat actccagctc ggacccggtc
     4501 atggcgcgat atcccatggc cgctgcccga tcgatctgcc gtcgcaggat ggaccggggc
     4561 gcctgcgcca cgggctggtg cgtccgctcg tcccggacgt cgcacaggac caacgcggtc
     4621 ttgtccagcc agctcgccac ccgcaaggtc gcgaaatcgg gcaccagatg gaaatccccg
     4681 tatccctttt cccagttgga gaaccgatac ccgggaaccg gctccatctc catatcaacg
     4741 gtcaagagat agtcgcacgc atgggttcct cgcttggccg cgtcgtccag gaaaaacccg
     4801 gcgtcgagcc gcttgcccat aaagcggccg taatgatccg tgaagaccag cagcacggtg
     4861 tcgatctgtc ccgcgcggac tcggcgcgac aactcgtccc gcgtca
//
LOCUS       DBNGTT010000468         4310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2538726, whole genome shotgun sequence.
ACCESSION   DBNGTT010000468 DBNGTT010000000
VERSION     DBNGTT010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2538726"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="AC16BC_10670"
     CDS             complement(<1..867)
                     /locus_tag="AC16BC_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242122.1"
                     /translation="MPAQAEEARLAVSALLGTDSNAYLSDASSTSYPATALILQTTVD
                     GAVKTDAGNRHRLALTVRGQQRHYFDFAGRVANPNRLFLDTAVSYRYAARPSLLVGFV
                     QTTSYARMQLFDTEGDTLPRELFSSWSGETRGYTQFVARRSLATLGAGIRVRDVNETA
                     GQPSLDQDGYFASLDGTFRFDASTVTLGYEYAVTHYDVLPALPVGPTATPLTLIQNAA
                     RSRIDLSLGARTKLGLDARQRWVIDPFESDLTYRQTDVTPTAQLRLPMDMVWDLSVGH
                     RARVYPGRDKRER"
     gene            complement(959..1966)
                     /locus_tag="AC16BC_10675"
     CDS             complement(959..1966)
                     /locus_tag="AC16BC_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR3242123.1"
                     /translation="MKTHERLVINTLMGFALLLSSPAVSVHAETLGAKVISVHGMVEM
                     QGGSPGQWVSAGAPAMLPPGSALRTGPESQAVIELDGSVITLYETSLIRIPTVASQAS
                     TGTRPLRHPALDRGRAVFDVTPTPDHVPFSVQTPTIVAGVKGTVFEVVSTGTSEAVYV
                     WRGLVEVSSRIDPDDVELVSAGQFTALDQLRLTLPSEIPFEREMPLGLEKHPDTGTER
                     THAVIASAPPVSGVLPTAAKPDTLPVDEHSAISSWPDAAHVDALPADIDHTAKFWDAA
                     DQTAVQGALDRAHDIQVVVSSGSLRSPAEKDPVVTSSTVDNTVSSTETITSDLLGVLG
                     F"
     gene            complement(2106..>4310)
                     /locus_tag="AC16BC_10680"
     CDS             complement(2106..>4310)
                     /locus_tag="AC16BC_10680"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3242124.1"
                     /translation="YVAGGFERPELMTYDWRLRAFPGPPRSDIVIVGIDSPSLKALDT
                     WPWPRSIHAALVDRLTQANARVIAFDVDFSTPRDPGADKVFARAVKRSGRVVLAAFDD
                     HRTLEGGAVVEYASLPYPTLRTAAQAVGSINFSLDSDGTVRRALIRKSLLGEDGESFA
                     MQVARAYLGALDSPVREADDGALEFTGHRAHLGGTTEFLIRFTGGPWTMPVVSAIDVL
                     HGTVPTPWLADKIVLVGATSLDLQDFRVTPFSGPMAGVEIQANAIATILSGIAPRRMA
                     SHHVLGGILVIVIAWTLFLTALRVWNPQEFGRRALWLSLAGAGAIVAIAAGSVILFAR
                     STIVLDMVPLLSAAAGQLCSSLLAGYLAAERGLELHRDNVEALYHMGEATQDRASLDQ
                     LADLLYVQARHILLVDRLGLDVWGQDDLFTREWRFRPVDAGSLPIPPDVYNDLVARVR
                     SSGLPVAASDLVVPRRRSEQGVWIRASLFVPLVAHNRVIGILHVHRDRATPFQQREAK
                     TLLTLATQAALNIESGRLLDDVRTLFQRSLEAFSTALDFKDNDTGGHSQRVAAGAREV
                     ALRMGLQGDALDHIAQGALLHDIGKIAVPDSILRKPGALSEEEWATMRQHPDTGFRML
                     KAIHVPDTIAAVVRQHHEKYDGTGYPLGLRGADISVGARIFSVVDFYDALSSDRPYRK
                     ALPLDQVVDQMRRDSGRHFDPSVLDAFLSIPEETLRAMRADAGNQLRYDRAA"
BASE COUNT          829 a         1311 c         1424 g          746 t
ORIGIN      
        1 tcgttcgcgc ttgtcccgtc caggatagac ccgggcacga tggccgacgc tcaggtccca
       61 gaccatgtcc atcggcagtc ggagctgggc cgtgggagtc acgtcggttt gacgataggt
      121 aaggtcgctt tcgaacggat caatcaccca gcgctgccgc gcgtccagcc ctaacttggt
      181 ccgcgcccca agggagagat cgatccggga ccgcgcggcg ttttggatga gtgtcaaagg
      241 tgtcgcggtg ggtccgacag gcaaggccgg caacacgtca taatgggtca cggcgtattc
      301 gtaccctaac gtgacggtcg acgcgtcgaa gcgaaaggtg ccgtcgagcg aggcaaagta
      361 gccatcctga tcgagagatg gctggcccgc tgtctcgttg acatcgcgaa cgcggattcc
      421 ggctccgagg gttgcgagcg aacgtcgcgc cacgaactgg gtatagcccc gggtttcgcc
      481 gctccaagat gaaaacagct cccggggcag ggtgtcgccc tcggtatcga acagctgcat
      541 tctggcgtac gaggtggttt gcacgaaccc caccaacagg gatgggcgcg ccgcgtaccg
      601 atatgacacg gccgtatcga ggaacaggcg attaggattc gcaacgcgcc ccgcaaaatc
      661 gaagtagtgt cgttgctgac cacggacggt cagagccagg cggtggcgat tgccggcgtc
      721 ggttttaaca gcgccatcga ctgtggtctg aaggatcaaa gccgttgcgg gatatgacgt
      781 agagcttgca tcgctgagat aggcgttgga gtcggtgccg agtaatgctg agacagcgag
      841 ccgcgcttcc tcggcctgcg cgggcatagc gcccagcaga acggtgagca ggcaaaatgc
      901 agagagccat cgtgggttga ctgtctccca cgatggctct cttacggacc atggcgaact
      961 agaagccgag gactccgagt agatcggagg tgattgtctc tgttgaagac accgtgttat
     1021 ccactgtcga cgaagtgacc acgggatcct tttcggccgg gcttctgagt gaaccgcttg
     1081 acaccacaac ttgaatatca tgcgcccgat caagggcgcc ctggaccgcg gtctgatccg
     1141 ccgcgtccca gaacttcgca gtgtgatcaa tgtccgctgg gagcgcatca acgtgagctg
     1201 catcaggcca cgagctgatg gcggagtgct catcgaccgg aagggtatcc ggctttgccg
     1261 cggttggcaa aacgcctgag accggcggcg cggacgcaat taccgcatgc gtgcgctcag
     1321 tgccggtgtc gggatgtttc tccagcccca acggcatttc tcgctcgaac gggatctccg
     1381 acggaagcgt caatcggagc tgatccagcg ccgtgaactg tcccgcggaa acgagttcga
     1441 cgtcgtcggg atcgatccgc gaactcactt cgaccaggcc gcgccacacg tacacggctt
     1501 ccgacgttcc ggtggagacg acttcgaaca ccgtgccttt gactccggcg acgatcgtag
     1561 gcgtctgcac tgaaaatgga acgtggtcgg gcgtcggggt gacgtcaaaa acggcgcgcc
     1621 cgcggtccag cgcggggtgc cgaagcggtc gcgttccggt cgaggcctgc gaagcgaccg
     1681 tcggaatgcg gatcaagctt gtctcgtaca gcgtgatgac cgacccatcg agttcaatga
     1741 cggcctgcga ctcggggccg gtccgaagcg ccgacccggg cggcaacatc gcgggagcgc
     1801 ccgctgaaac ccattggccc ggcgagccgc cctgcatctc gaccatgccg tgtacgctga
     1861 tgacttttgc gcccaacgtc tccgcgtgca ccgaaaccgc cggtgacgaa agaaggagcg
     1921 cgaatcccat gagcgtgttg attacgagcc gttcatgtgt cttcatgtct ggccctcctc
     1981 gatgccgaaa ccacgacccc gtcccggata ttccaccgac ggcgcgacga gacacagttg
     2041 taatcagaat agctcgtggt ggtatgtcag ccgttcccca ggtgggtagc cggctccaca
     2101 gccagttagg ccgcgcggtc gtaacggagc tggtttcccg catcggcgcg catcgcgcgc
     2161 aacgtctcct ccggaatgga caaaaatgcg tcgagcacgg acgggtcaaa atggcggccg
     2221 ctgtctcgac gcatttgatc cacgacttgg tcgagcggca gggccttgcg gtagggccgg
     2281 tcgctcgata gggcgtcgta aaagtccacc acggaaaaaa tcctggctcc caccgaaatg
     2341 tccgccccgc gaaggccgag cgggtatccc gtgccgtcgt atttctcgtg gtgctgacgc
     2401 acgacggcgg cgatggtatc gggaacgtgg atcgctttga gcatccgaaa gccggtgtcg
     2461 ggatgttggc gcatggtggc ccattcctcc tcggacagcg cccctggctt gcgaaggatg
     2521 ctgtccggga ccgcgatctt cccgatgtcg tgcaagagcg cgccctgcgc aatgtggtcg
     2581 agagcgtctc cttgaaggcc catgcggagc gccacttcgc gcgcgccggc ggccacgcgc
     2641 tgcgagtggc cgccggtgtc gttgtccttg aagtcgagcg cggtggaaaa ggcctcgaga
     2701 ctccgttgaa acaaggtccg aacgtcgtcc agcagccggc cgctctcgat gttcaacgct
     2761 gcctgcgtcg ccagggtgag caacgtcttg gcttcacgtt gctggaacgg agtcgcccga
     2821 tcgcgatgga cgtgcagaat tccgatgacg cggttgtgcg cgaccagcgg gacaaataag
     2881 ctggcccgga tccatacacc ctgctcgctc cggcggcgcg gcaccacgag atcggatgcc
     2941 gcgaccggga gtccgctgga gcggacgcgc gcgaccaggt cgttgtagac gtccggagga
     3001 atcggcagag agcccgcatc gacgggccgg aatcgccact cgcgagtgaa cagatcgtcc
     3061 tgtccccaga cgtcgagccc cagccggtct accaggagga tgtgtcgggc ctggacgtag
     3121 agcagatcgg ccaattgatc gagcgaggcg cgatcctggg tggcttcacc catgtgatac
     3181 aaggcctcga cgttgtcacg gtgcaactca aggccgcgtt cagccgcaag atagccggcg
     3241 agcagtgagg agcagagttg tccggccgcc gccgacagca gcggcaccat atcgaggacg
     3301 atggtcgacc ttgcgaagag tatgacgctc cccgcggcga tggcgacgat cgcgccggcc
     3361 cccgcgagcg acaaccacag ggcgcggcgg ccaaactctt ggggattcca gacacgcagg
     3421 gccgtcaaga acaacgtcca tgcgatgacg atcaccagga tgccgcccaa cacgtgatgt
     3481 gaggccatgc ggcggggtgc gattccggac aggatagtgg cgatggcgtt ggcctggatc
     3541 tccactccgg ccatggggcc ggaaaacggc gtgactcgaa agtcctgcaa gtcgagcgat
     3601 gtggcgccga ccaagacgat tttgtcggca agccacggag tcgggaccgt gccgtgtagg
     3661 acgtcaatgg cggaaaccac cggcattgtc cagggtccgc cggtgaagcg gatgagaaac
     3721 tcggtggttc cccctaagtg ggcccggtgc ccggtgaatt cgagcgcgcc gtcatccgcc
     3781 tcacggaccg gggagtcgag cgcgccgagg taggcccgcg caacctgcat cgcgaaactt
     3841 tcgccatctt cgccgagcaa agattttcga atcagcgcac gacgcaccgt tccgtcgctg
     3901 tcgagcgaaa aattgattga acccacggct tgcgcggccg tccgtaacgt cggatagggc
     3961 aagctggcgt attccacgac tgcgccgccc tccagcgtgc ggtggtcatc gaaagctgcg
     4021 agtactacgc gccctgaacg cttgaccgcg cgtgcgaaga ccttgtccgc tcccgggtcg
     4081 cgtggcgtgc tgaaatcgac gtcgaacgcg atgacgcggg cgttggcctg ggtaagccgg
     4141 tcgacgagcg ccgcgtgtat ggatcgcggc cagggccagg tatccagggc cttgagactc
     4201 gggctgtcga tgccgacgat gacgatgtcg ctgcgcgggg gtcccgggaa cgcgcgcagc
     4261 cgccagtcgt acgtcatcag ctccgggcgc tcgaagccgc cagcgacgta
//
LOCUS       DBNGTT010000469         5157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2795839, whole genome shotgun sequence.
ACCESSION   DBNGTT010000469 DBNGTT010000000
VERSION     DBNGTT010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2795839"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1038)
                     /gene="bluB"
                     /locus_tag="AC16BC_10685"
     CDS             complement(337..1038)
                     /gene="bluB"
                     /locus_tag="AC16BC_10685"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HIR3242125.1"
                     /translation="MYEAIYRRRDVRRFLPRPVSDKVLRRILRAAHQAGSIGVMQPWN
                     FLVVRDAEIKRRVKQEFLRASEEVGRGAEDGQQHMDQSLTAEAIEQAPINLAVTCDRS
                     RRPHVASRHDTPDPDLVETCCAIQNLWLAGRAEGVGVGGVNILDPSAIAGILGLPAGI
                     VLVAYLCMGHVARFPDAPTLETAGGESRLSLESVLFYDQWGNRGQETHHHEELTDSTP
                     SRRTGRSLPRGSREP"
     gene            complement(1108..1674)
                     /locus_tag="AC16BC_10690"
     CDS             complement(1108..1674)
                     /locus_tag="AC16BC_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242126.1"
                     /translation="MSSLARAIDWRSHVWFAMLVAASIGLSTTFACATPLVAFGAAAA
                     LTLPSRDALRLIGVVWFTDQVFGYTVLHYPQTANSVAWGLIMGAAAVLATVAAQRALQ
                     RSEGRPKTVAAFAAFLVGFVVHQVALAVPAVMGLGGLGGFKPAVIARVFMVNAGTLVG
                     LMVLNYLGRAVGLAAQSSTPFTRTRRPA"
     gene            complement(1699..3576)
                     /locus_tag="AC16BC_10695"
     CDS             complement(1699..3576)
                     /locus_tag="AC16BC_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3242127.1"
                     /translation="MVAFAQGVHAQQPKRDADIVVTATRLPTPAAEVASSVTVITREE
                     IERKQQRTLADVLRDVPGLSLVQTGGPGGLTSVFMRGTNANHTKVFIDGIDVGDPSSA
                     GGDFDFAHLPAEDVERVEVLRGPQSGLYGSDAIGGVINVVTKRRSGATRLTVGVESGS
                     FGTFNQTGSASGAVSRLHYALDVGHLHSGDTPVTPQDLLPTGRNPIGDFYDNKTVSAK
                     LGAGLTDSFEMDVVARYIDTELRFTGEDFSTFPSSPAAEQSQADTGQFFTRGTAHALL
                     FDGALDQTLGVAYTRHYRRYLSPDTGLGPVDPVTYRGNRIKWDWQGNLFIAATQILTL
                     GAESQREEITTPVSAEATTNAGFLQMQSSLDKRLFSTISLRYDDHDRFGGETTYRIAP
                     AMLMRGTGTKLKGSVGTGFKAPALDQLFHDYPAFGFFANPDLKPEESFGYDLGFEQAL
                     TRAHVQVGATYFHNDVDNLITINNAGTSYANVAEATTSGVETFLAHELSDRLSWRADY
                     TYTKAEDAVSGEELLRRPKHKASVDARWQVTDAGTLSATVLYVGSRIDRTRDFTVPRF
                     EAAGYTIVNLAGSYDLSRVLMAFARIENLFNREYEDPIGFLRPGFGAFAGVQAVLGGT
                     R"
     regulatory      complement(3646..3827)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3882..4634)
                     /gene="cobS"
                     /locus_tag="AC16BC_10700"
     CDS             complement(3882..4634)
                     /gene="cobS"
                     /locus_tag="AC16BC_10700"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR3242128.1"
                     /translation="MDRFAMAWAFLTIVPVRLQPHSPDERACARSLAAFPLVGLVLGV
                     GLAGASVVLDRFFPHSLAVVVIVFLLMWITGGLHLDGVADTADACGARSKADALRIMR
                     SSTIGTFGSLALITVLGLKIAGLATLPPDGLAPALVAMPVLGRWAQVQMTTGHPSARE
                     DGAARRFIVGATRTDYAIATAFALPVVVFAMGLVGLLAAAVVWAAAAWFGRSMTRRLG
                     GVTGDTIGAAGEGAEVLAVLTWAGAVHALGAA"
     gene            complement(4634..>5157)
                     /locus_tag="AC16BC_10705"
     CDS             complement(4634..>5157)
                     /locus_tag="AC16BC_10705"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR3242129.1"
                     /translation="TTPSSAITAVMTGAIPRAVTGRGTGLDDPAWTRKILVIERALAV
                     NQPDPNDPLGVLTALGGLEIAALVGAMLGAARCRVPVVLDGFISGAAALIAVGLESSL
                     RGYLLASHRSVEPGHALALDRLGLKPLLELDLRLGEGTGAALAIGILDAAVKIVNEMA
                     TFHEAGVSERADP"
BASE COUNT          915 a         1702 c         1608 g          932 t
ORIGIN      
        1 ctacgacggt acatgatctc ctcgtagagt tctcgaactt gagtattcag gggcatattg
       61 aagccaaccg tttagaacct tgtcccgaac gacaacggcg ctgccgcgct caaatcattt
      121 gtttttgacc ctcagcagtc gcctcgaaaa agtctcatca aatcaatcgc tgtttccgtc
      181 agggtacaag gaggtttttg gaccactttc aaggccacct gtttcagcgc gtctttcggc
      241 agatcacgtc ggtgaaaggg actcgacgtg tggtcatgca gtagtggtga ccggataatc
      301 gtagcggatt gattctgacg gggtcaagcg cttacgtcac ggttcccgtg atccacgggg
      361 aagagagcgg ccagtgcgac gtgacggcgt actgtcggtc agctcctcgt ggtggtgagt
      421 ctcctgacca cggttacccc attgatcgta gaagaggacg gactccaacg acagccggct
      481 ttcgccgcct gcggtctcga gcgttggggc atcggggaac cgtgccacgt ggcccatgca
      541 gaggtatgcc accaacacga tgcccgcggg gagcccgagg attcctgcta ttgcggaggg
      601 atcgaggatg ttgacccctc ccacgcccac gccttctgcg cgcccagcca gccagaggtt
      661 ttgaatcgcg caacaggtct caaccaggtc gggatctggc gtgtcgtgac gactcgcgac
      721 gtgcgggcgc cgcgatcgat cgcacgtcac ggccaggttg atgggcgctt gctcaatggc
      781 ttccgccgtg aggctctgat ccatgtgttg ctgcccgtcc tcggcccccc gcccaacctc
      841 ctcgctcgcg cgtagaaatt cttgcttgac ccgacgtttg atctccgcgt ctcggacgac
      901 caggaagttc cacggctgca tgacgcctat tgaccctgct tggtgcgcgg cgcgcaggat
      961 ccggcggagc accttgtccg agaccggtct cggcagaaac cgtcgcacgt cgcgacggcg
     1021 atagatggcc tcgtacactc cccgcctcca gagcggcgga aatgcgttgg atgtcaactc
     1081 acgcacaggg gtcttcgtcg ttcctagtta cgcaggtcgt cgtgttcgag tgaacggtgt
     1141 cgaggattgc gcggccaggc cgacagcccg ccccagatag ttgaggacca tgagtcccac
     1201 caaggtgccg gcgttgacca tgaagacccg tgcgatgacc gccggcttga agcctcccag
     1261 gcccccgagc cccatgactg ccggaaccgc gagggcgact tggtgcacga caaacccgac
     1321 cagaaaagcg gcaaacgcgg cgacggtctt gggccggcct tccgaacgct gcagggcgcg
     1381 ttgcgcggcg acggtcgcga gcacggctgc ggcgcccatt atcaggcccc acgccacgct
     1441 gttcgcggtt tggggatagt gcagcaccgt gtatccaaac acctgatccg tgaaccagac
     1501 cacgccgatc agccgcaagg catcgcgtga cggcaacgtc agggcagcgg ccgcgccgaa
     1561 cgccaccaag ggcgttgcgc aggcgaaggt tgtgcttagt cctatgctgg cggccaccaa
     1621 catcgcgaac cacacgtggg aacgccaatc gattgcgcgt gcgagcgaag acatgttgtt
     1681 cctccttctc agaatgagtt atcgcgtccc accgagtacg gcttgcactc cggcgaatgc
     1741 gccgaatccc gggcgcagga aaccgatcgg gtcctcgtac tcccgattga acagattctc
     1801 gatgcgcgcg aatgccatga ggacacggct caagtcgtat gatcccgcga ggtttacgat
     1861 cgtgtacccg gccgcctcaa aacggggcac cgtaaaatct ctggtgcgat cgatacgcga
     1921 acccacgtag agcacggtcg ctgacagcgt cccggcgtcc gtgacctgcc accgggcgtc
     1981 cacactggcc ttgtgtttgg gccgacgcag cagttcttcc ccgctgacgg catcttcggc
     2041 cttggtatag gtgtagtccg cgcgccacga caggcgatcg gacagctcgt gcgccaggaa
     2101 ggtctcgacg ccggatgtgg tggcttccgc gacgttcgcg taggacgttc cagcattgtt
     2161 gatcgtgatg aggttgtcga cgtcgttgtg aaaatacgtg gcgccgacct gaacgtgcgc
     2221 tcgggtgagg gcttgctcga accccaggtc gtacccgaag ctttcttccg gcttgaggtc
     2281 gggattggcg aagaacccga acgcggggta gtcgtggaaa agttggtcga gtgcgggagc
     2341 tttgaacccg gttcccacgc tgcctttgag tttggtgccg gttccccgca tcagcatggc
     2401 gggcgcgatg cggtaggtgg tctcaccgcc gaaccggtcg tgatcgtcgt agcgcagact
     2461 gatggtgctg aacaggcgtt tatcaaggct cgattgcatc tggaggaagc ccgcattcgt
     2521 cgtggcctcg gctgagaccg gggtggtgat ctcttcccgc tggctctcag ctccaagagt
     2581 cagaatctgt gtcgccgcga tgaacagatt gccctgccag tcccacttga tccgattgcc
     2641 gcgataggtg acgggatcaa ccggtccgag ccctgtgtcc gggctcagat accggcggta
     2701 gtgacgcgtg taggccaccc cgagcgtttg gtccaaggcc ccgtcgaaaa gcagggcatg
     2761 cgccgtgccg cgcgtgaaga actgtcccgt atccgcctgg ctctgctctg cggccggtga
     2821 gctcggaaac gtactgaagt cctcgccggt gaatcggagt tcggtatcga tgtaccgcgc
     2881 caccacgtcc atctcgaacg agtcggtcag accggctccg agcttggccg agacggtctt
     2941 gttgtcgtag aaatcaccga tcggattcct gcccgtcggc aagagatcct gcggggtgac
     3001 gggcgtgtcc ccggagtgga gatggccgac atcgagggcg tagtggagac gactcacggc
     3061 gccgctcgcc gagccggtct gattgaaggt gccgaacgac ccgctttcca cgcccaccgt
     3121 caaccgagtc gctccgctcc tgcgtttcgt caccacgttg atgacgccgc cgatcgcgtc
     3181 ggagccgtac agcccgctct gcgggccgcg cagcacttcg acgcgttcga cgtcctcagc
     3241 cgggaggtgc gcgaaatcaa agtcgccgcc ggcggaactg gggtcaccga cgtcgatgcc
     3301 gtcgatgaac accttggtgt ggttggcgtt ggtgccgcgc atgaagaccg aggtcagtcc
     3361 tcccggtccc ccggtttgga ccaggctgag ccccggaaca tcgcgcagca catcggccaa
     3421 tgtccgttgt tgcttgcgtt cgatctcttc gcgggtgatc acggtcacgc tgctcgccac
     3481 ctcggctgca ggtgtgggta gacgcgtggc ggtcaccacg atgtcggcgt ctcttttcgg
     3541 ctgttgcgcg tgtacgccct gcgcgaatgc gaccatccct aaaagacaca ggactcccct
     3601 gctccaacgg ttcacgtgct tctccttccc cctcggaaag tccgagcgtg tgcggtgagc
     3661 caatcggtct cctgactcgt cgggtcgtcc tccctctgcg ccttcccgtc gttcgacagt
     3721 ggcacgtgca gaggtcgtcg ccgatcacag ttgcggggca gtggcggatt tgcaccgcct
     3781 tcccggcttt ggctcacctg ttgtggatat gacttacgcc attcagcgtc tccggttcgt
     3841 cgtctcgctc tttcagccct cgatttccaa cagcatgcgc cttacgcggc gccgagcgcg
     3901 tgaaccgccc cggcccacgt cagaaccgcg agcacttcgg cgccctcgcc cgccgcgccg
     3961 atggtgtcgc cggttacgcc gcccagccgg cgggtcatgc ttcggccgaa ccacgccgcc
     4021 gcagcccaca ccacggcggc cgccagcagg ccgaccagtc ccatggcgaa caccacaacc
     4081 ggcaacgcga acgcggtcgc gatcgcatag tccgtgcggg tcgcgcccac gataaagcgt
     4141 cgggccgcgc cgtcctcccg cgcagacggg tggcccgtgg tcatctgcac ttgcgcccac
     4201 cgtcccaaca ccggcatggc caccaatgcc ggcgccaacc cgtccggggg cagcgtcgcc
     4261 agcccggcga tcttgagccc gagcaccgtg atgagcgcga ggctgccgaa cgtgccgatg
     4321 gtggaactcc tcatgatccg gagcgcgtcg gccttcgagc gcgcgccgca cgcgtccgcg
     4381 gtatcggcca cgccgtcgag atgcagccct ccggtgatcc acatcaatag aaaaacgatg
     4441 acaaccaccg ccaaggagtg cggaaagaac cggtccagta cgaccgacgc cccggccagc
     4501 cccacgccca gcacgagccc caccagggga aacgcggcca gcgatcgggc gcacgcgcgc
     4561 tcgtccggtg aatgcggctg caggcgcacc ggaacaatgg tcaggaaagc ccacgccatg
     4621 gcgaagcgat ccatcatggg tccgctcgct ccgacacccc ggcctcgtgg aacgtggcca
     4681 tctcgttcac gatcttcacc gccgcgtcca ggatgccgat cgccagcgcc gcgcccgtgc
     4741 cctcgcccag acgcagatcc aactctaaga gcggcttgag gcccaatcga tcgagcgcaa
     4801 gggcatgacc cggctcgacc gaccggtgcg acgccaggag ataacctcgc agcgaggact
     4861 ccagccccac ggcgatcagg gccgcggccc cggagatgaa gccgtcgagc accaccggca
     4921 cgcggcaacg cgccgcgcca agcatcgcgc cgaccaacgc cgcgatctcc aggccgccga
     4981 gcgcggtcag gacccccaag ggatcattgg gatcgggctg gttgaccgcg agcgctcgct
     5041 cgatgaccag aatttttcga gtccaggccg ggtcgtccaa ccctgttcca cgacccgtca
     5101 ccgcccgtgg gatcgcaccg gtcatgaccg cggtgatcgc gctgctgggc gtagtgt
//
LOCUS       DBNGTT010000470         4108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_366797, whole genome shotgun sequence.
ACCESSION   DBNGTT010000470 DBNGTT010000000
VERSION     DBNGTT010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_366797"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="AC16BC_10710"
     CDS             <1..1029
                     /locus_tag="AC16BC_10710"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3242130.1"
                     /translation="HALARRAERPNPVAGVVARALLRPALFRAMTKFAAATQSLWDRP
                     LPRRVLDAISRPVLHRLAPTARLPHDLRLPRLARTTLRERHHRLTDEAGATGATAYFH
                     GCAAEYFDDGVGDAVIRLLERRGLGPVLPRQRCSGTPIQTYGQTGPLMGAARFNLDSL
                     ERFDTVVTGCASCTLMLRDYPTLFTGEDQRRAQALARKVRHITEILAVDSIAPSPAPP
                     IAGSVSTVTYHSSCHLRAAGISKEPRRVLATLPGMQFVEMPDADRCAGGAGTFIVKNP
                     ELSDQIVARKRRAVEASGADVVATSCPACLIRLRAALPDRVRTAHVAQLADEAESAAA
                     VVPGRSAP"
     gene            1048..1773
                     /locus_tag="AC16BC_10715"
     CDS             1048..1773
                     /locus_tag="AC16BC_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3242131.1"
                     /translation="MQLRVMTYNIHSGFGQDGYDLQAIRRAIEHERVDLVALQEVDFG
                     LRRTGYVNQARWLGDRLGLHAIAGGTRRQGRYGNALLTRWPATFVMNHNLTVWPQPGR
                     ACLEVHLDTPKGTLRCYVTHLGLLPQERTVQVTRLIEEIITKDAEHAPVVVMGDFNTV
                     SRSRVSGRLRARFTDVFHAVGNGRAATFHARLPGVRFDYIYTSPRLTPLSCHVAASPW
                     SERASDHLPLVAALSVAFGAKRS"
     gene            complement(1886..>4108)
                     /locus_tag="AC16BC_10720"
     CDS             complement(1886..>4108)
                     /locus_tag="AC16BC_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3242132.1"
                     /translation="SVLVELKAVEDAYPFYGRVETAPDRPLASWLGGGPPFGAVVHEA
                     LLIRLGLSPGDRFRLGDAEFVAAAVLRAEPDRTAGAFSLGPRVLIARAALDHTALVRT
                     GSRVRYRTLVALPAEAEPAERVRDALRAELADEPVQIAAFADAQPQLRRSLDRVGAYL
                     GLVGVTALLLGGVGVAGSARALLAERWRTIALLKCVGAEARTVAWATLGELAVLGLCG
                     GALGAGFGALGQRVLTAAAAAFTGLSPSGAASPWPYAQGAMVGVLTGVAFSLGPLLAA
                     ARAPAAAVLRHEASPLRPGRAAWWLGAVGSAAAGGLAWWQTRSAATAALYVGGIAAAA
                     LVLTAAAWGLGRLVRQAPAPPSFAWRRGLGALTRPGAHAAAAMLSIGLGVTAVLVVGF
                     VERAIRAELTERVPRDAPSLFFIDIQPDQRDGFAALLAARPTAADVVPVARGRLRVVG
                     DRAVGTESGSGGESDSRWYLTREYVVTVRPDLPRGNAVTKGRWWDALPSDDQTGARVS
                     VEQELARRLGIDVGSTIVLDVQGVPLEARVASLRSVDWDNRGLNFFLLFSPGALDEVP
                     ITYVAAARAAAGDEIALQQAVVAAFPNVTAIPIGEVLASVARVIERVSSAVRGVALLV
                     AAAGFIVLGGALAAGRAARLREAMILKTLGAGRGAMTRALTVEFGLVGAAAGVLGTGM
                     AAALAWAMARWLFEVPWVWAPDLAFNGVALTVAGTVAVGLGGTYRLLGKRPFPVLRGE
                     "
BASE COUNT          627 a         1638 c         1334 g          509 t
ORIGIN      
        1 cacgcgctcg cgcgccgcgc ggagcggccc aaccccgtgg ccggcgtagt ggcgcgcgcg
       61 ctgcttcgcc ccgcgttgtt ccgcgcgatg accaagttcg cggccgcgac ccagtcgctc
      121 tgggaccggc ccctgccgcg acgcgtcctt gacgcgatca gccgtccggt gttgcaccgc
      181 ctcgcgccca ccgcgcggct gccccacgac ctgcggctcc cgcgcctggc ccggaccacg
      241 ctgcgcgagc gccaccaccg tctgaccgac gaggcgggag ccaccggcgc caccgcgtat
      301 tttcacggct gcgcggccga gtacttcgac gacggggtgg gcgacgcggt gatccgcctg
      361 ttggagcggc ggggcctggg cccggtgctc ccgcgccagc gttgctccgg cacgccgatt
      421 caaacctacg gccagaccgg tccgctcatg ggggcggcgc gtttcaacct cgacagcctg
      481 gagcgcttcg acaccgtggt gacgggttgc gcgtcgtgca ccttgatgtt gagggactac
      541 ccgaccctgt tcacgggcga agaccagcgc cgcgcgcagg ccctggcccg aaaggtgcgg
      601 catatcaccg agattctcgc tgttgactcg atcgccccct cgcccgctcc ccccatcgcc
      661 ggttcggtca gtactgtcac ttaccactcc tcctgccact tgcgcgccgc gggaatctcc
      721 aaggaaccgc gccgcgtgct cgcgaccttg cccggcatgc agttcgtgga aatgccggac
      781 gcggatcggt gcgcgggagg cgcgggcacg ttcatcgtga aaaatcccga gctgtcggat
      841 cagatcgtcg cccgcaagcg gcgcgcggtc gaggcctcgg gcgccgacgt ggtcgcgacc
      901 agttgtccgg cctgcctgat ccgcctgcgc gccgcgctgc ccgaccgggt ccgcaccgcg
      961 cacgtggcgc aactcgcgga cgaagccgag tccgcggccg ccgtggtccc cggacgatcc
     1021 gcgccctgag tcgcgcccct gaccgccatg caactacgcg tcatgactta caacatccac
     1081 agcggattcg gccaggacgg gtacgacctg caggcgatcc gccgcgcgat cgaacacgaa
     1141 cgcgtggatc tggtggcgct gcaggaagtg gacttcggac tgcggcggac cgggtacgtg
     1201 aatcaagcgc ggtggctggg cgaccggctc ggcctgcacg ccattgcggg cggcacccgc
     1261 cgccagggcc gctacggcaa cgcgctgctc acgcgctggc ccgccacgtt cgtgatgaac
     1321 cacaatctca cggtgtggcc ccaacccggc cgcgcgtgcc tggaggtcca cctggacacc
     1381 ccgaagggca cgctccgctg ttatgtcacc cacctcggcc tgttgcccca ggagcgcacc
     1441 gtgcaggtca cgcggttgat cgaggagatc atcaccaagg acgcggaaca cgccccggtg
     1501 gtggtgatgg gcgacttcaa caccgtgtcg cgcagccgcg tgtcgggccg gctgcgcgcc
     1561 cggtttaccg acgtgttcca cgcggtgggc aacggccgcg ccgcgacgtt tcacgcgcgc
     1621 ctgccgggcg ttcggttcga ctacatttac accagccccc gcctcacccc gctgtcgtgc
     1681 cacgtggcgg ccagtccgtg gtccgagcgg gcctcggacc atctgccgct cgtggccgcg
     1741 ttgagcgtgg ctttcggcgc gaaacgctcg taagcacgtc cggccatgcc gtccgccgga
     1801 tcgcttcgcg cgccggaccc gggcgggctc agaccgggtc ccttgttcta atggccgcgt
     1861 gccgggtcgt tcgcgcgggc actgatcact cgccccgcag cacgggaaag gggcgcttgc
     1921 ccagcagacg ataggtgccg cccaggccca cggcgacggt ccccgcgacc gtgagcgcca
     1981 cgccgttgaa cgcgaggtcc ggggcccaga cccagggcac ctcgaacaac caccgcgcca
     2041 tcgcccacgc cagcgccgcg gccatgcccg tgccgagcac gccggccgcg gcccccacca
     2101 acccgaattc gaccgtcaac gcccgcgtca tggcgccgcg accggccccc agcgtcttga
     2161 gaatcatcgc ctcccgcaac cgcgccgcac gcccggccgc caacgcgccg ccgagcacga
     2221 tgaacccggc cgccgccacc agcagcgcca ctcctcgcac ggccgaggag acccgttcga
     2281 tcacgcgggc caccgaagcc agcacctcgc cgatcggaat cgcggtgacg ttggggaagg
     2341 ccgccaccac cgcctgttgc aacgcaatct cgtcgccggc cgccgcccgc gccgcggcca
     2401 cgtacgtgat cggcacctcg tccagcgcgc ccggggagaa caggagaaag aaattgaggc
     2461 cgcggttgtc ccaatccacc gaccgcaggc tggcgacgcg cgcctccagc ggcacgccct
     2521 gcacgtcgag cacaatcgtc gatccgacgt cgatccccag acgtcgggcc aattcctgtt
     2581 ccaccgacac gcgcgcaccc gtttggtcgt cagaaggcag cgcgtcccac caccgtccct
     2641 tcgtcacggc gttaccgcgc gggaggtcgg gccgcacggt caccacgtat tcacgggtca
     2701 agtaccagcg cgagtcggac tctcctccgg agccggactc cgtcccgacc gcccgatccc
     2761 ccacgacccg caatcgcccg cgagccaccg gcaccacgtc cgcggcggtc ggacgcgcgg
     2821 ccagcaacgc cgcgaatccg tcgcgctgat ccggctgaat gtcgatgaaa aacagactcg
     2881 gcgcgtcgcg aggcacgcgc tcggtcaact cggcgcggat cgcccgctcc acgaaaccca
     2941 ccaccagcac cgcggtcacg cccaacccga tcgaaagcat ggcggccgcg gcgtgcgcgc
     3001 ccggccgcgt cagcgcgccc agtccgcgcc gccacgcaaa cgagggcggc gcgggcgcct
     3061 ggcgcaccag gcgtcccagt ccccacgcag ccgcggtcaa caccagagcc gcggccgcga
     3121 tgcctcccac gtacagggcg gcggtcgccg cggaacgcgt ctgccaccac gcaagacctc
     3181 ccgcggccgc cgagccgacg gcccccagcc accacgccgc gcgtcccgga cgcaacggcg
     3241 acgcctcgtg ccggagcacc gccgccgcgg gcgctcgcgc ggccgcaaga agcggcccca
     3301 gcgaaaacgc cacccccgtg agcacgccca ccatcgctcc ttgcgcgtac ggccaggggc
     3361 tcgccgcacc gcttggggag agtccggtga acgctgccgc ggccgcggtg agcacgcgct
     3421 gccccagcgc gccgaatccg gctccgagcg cgcccccgca caatccgagg accgccaact
     3481 ctccgagcgt ggcccaggcc acggttcgag cctcggcgcc gacgcacttg agcagcgcga
     3541 tcgttcgcca gcgctcggcc agcaatgcgc gggcgctgcc cgccacgccc acgcccccca
     3601 agagcagcgc ggtcaccccc acgagtccca agtacgcgcc gacgcgatcg agagaacggc
     3661 gcaattgcgg ctgcgcgtcc gcgaaggccg cgatctgcac gggctcgtcc gccagttccg
     3721 cgcgcagggc gtcgcggacg cgctcggccg gctcggcctc cgctggcaac gccaccaagg
     3781 tgcgataccg cacccggctc cccgtccgca ccaacgccgt gtgatcgagc gccgcgcgcg
     3841 cgatcaggac ccgtggcccg agactgaacg ccccggccgt gcggtcgggt tcggcgcgca
     3901 acaccgcggc cgcgacgaac tccgcgtcgc ccagacggaa ccggtcgccc ggggacaatc
     3961 ccaggcggat caggagcgcc tcgtgcacca cggcgccgaa cggcggcccg ccgcccagcc
     4021 acgacgccag cggacgatcc ggcgcggtct cgacccgccc gtagaacgga tacgcgtctt
     4081 cgaccgcttt gagttccacc agcaccga
//
LOCUS       DBNGTT010000471         2653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2667814, whole genome shotgun sequence.
ACCESSION   DBNGTT010000471 DBNGTT010000000
VERSION     DBNGTT010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2667814"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="AC16BC_10725"
     CDS             complement(<1..506)
                     /locus_tag="AC16BC_10725"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981919.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3242133.1"
                     /translation="MIIVLKPEATQAQIEHIMEKIRERGLSPHLSKGQERSVIGVIGD
                     ERTIQNVPLAVFPGVESVTPILSPYKLVSRDFRKDTTVITVGDGAVIGGNTVPVMAGP
                     CAVETKAQVAGVAKAVKGAGARVLRGGAFKPRTSPYAFQGLAEEGLQYLAEAKKVTGL
                     PIITEVVDP"
     gene            complement(611..1705)
                     /gene="hisC"
                     /locus_tag="AC16BC_10730"
     CDS             complement(611..1705)
                     /gene="hisC"
                     /locus_tag="AC16BC_10730"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR3242134.1"
                     /translation="MAFRVRPELSRIAPYVPGKPVAEVEREYGVSHAIKLASNENPLG
                     PSPAALKAVREVLEGLHRYPDGSGVALRAAIAARLDVRPDQVILGNGSDEVVALLCNV
                     LLTPGDEAVMAAPTFGIYRIAVLSHQGTPVEVPLQAGRHDLDAMLGRVTDRTKLFFIC
                     NPNSPTGTALSRREVLDAIDRLPPHVVAVCDHAYEDYATAADFPFGGSLAQGGRPVVI
                     LRTFSKIYGLAGLRIGYGVGPAELIGWMNRIRLPFNASTVAQVAAIAALGDDAHVRAS
                     RMVNAAGKTYLTKECARLGLPVLPTEANFVYLDVGRDARGVFERLLREGVIVRHFDGR
                     WLRITIGLPEENTRCMAALERVLSDESGMG"
     gene            complement(1717..>2653)
                     /gene="pheA"
                     /locus_tag="AC16BC_10735"
     CDS             complement(1717..>2653)
                     /gene="pheA"
                     /locus_tag="AC16BC_10735"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3242135.1"
                     /translation="VPRREQEIFERLERENPGPFPNDGVRAVFREIMSASLALEQPLK
                     VAYLGPPGTFTHVASLKQFGASAAAIAVNTIKDVFNEVERGRADYGVVPIENSTEGVV
                     THTLDLLADSQLKIAGEIVQEISHYLLSRSGVLADVKRIYSHPQPIAQCRGWLAQHVP
                     NIPLVEVSSTARAAEMCRDEPEAAAIASEVAARLYGLSVIQKRIEDNPNNTTRFLLIA
                     PRAPERTGRDKTSIMVSVQDRVGALYDMLKPFAEFGLSLTKIESRPSRRKAWEYYFYI
                     DVEGHVDDEPVKKALDALRPHCQVLKVLGSYPRIA"
BASE COUNT          436 a          935 c          843 g          439 t
ORIGIN      
        1 gggtccacga cctcggtgat gatggggagg cccgtgacct tcttggcctc ggccaggtac
       61 tgcaaccctt cctcggccaa gccctggaac gcgtacggag acgtgcgcgg cttgaacgcc
      121 ccgccgcgca acacccgcgc gcccgccccc ttcacggcct tggccacgcc ggccacctgg
      181 gccttggtct ccaccgcgca cggcccggcc atgaccggca ccgtgttccc gccgatcacc
      241 gcgccgtcgc ccaccgtgat caccgtggtg tctttgcgaa aatcgcggct cacgagtttg
      301 tacggcgaga gaatcggcgt gacgctctcc accccgggga acaccgccaa gggcacgttc
      361 tggatcgtgc gctcgtcgcc gatgaccccg atcacgctcc gctcctgccc cttggacagg
      421 tgcggggaga ggccgcgctc gcggatcttc tccatgatgt gctcgatctg ggcctgcgtg
      481 gcttctggtt tcagtacgat aatcatcggt cgaactccct gttattgaaa aacatgaatc
      541 ggggaaccgg ggaaccgggg aaccggcgaa aaccacgacg gtctacgcgc tttttccccg
      601 tttctccgtt tcaccccatc cccgattcgt cactcagcac ccgttccagc gccgccatgc
      661 aacgcgtatt ttcttccggt agcccgatgg tgatccgcaa ccaccggccg tcgaagtgcc
      721 gcacgatcac gccctcccgc aacaggcgtt cgaacacgcc gcgcgcgtcg cggcccacgt
      781 cgagatacac gaagttggcc tcggtcggca acaccggcaa ccccaggcgc gcgcactcct
      841 tggtcagata cgtcttgccc gcggcgttga ccatgcggct cgcgcgcacg tgcgcgtcgt
      901 cgcccaacgc cgcgatcgcc gcgacctgcg ccacggtgct cgcgttgaag ggcagccgga
      961 tgcggttcat ccacccgatg agctccgcgg gccccacgcc gtacccgatc cgcagcccgg
     1021 caagaccgta gatcttcgag aacgtgcgca ggatcacgac cggacgcccc ccctgcgcca
     1081 gactcccgcc aaacgggaaa tccgcggcgg tcgcgtaatc ctcgtacgcg tgatcgcaga
     1141 ccgccaccac gtgcgggggc agccggtcga tcgcgtcgag cacctcgcgg cgcgagagcg
     1201 cggtgccggt cgggctgttg ggattgcaga tgaagaacaa cttggtccgg tcggtcacgc
     1261 gccccagcat ggcgtccaga tcgtgccgac ccgcctgcaa cggcacttcc accggcgtgc
     1321 cttggtgcga caagaccgcg atccgataga tcccgaacgt gggcgcggcc atcaccgcct
     1381 cgtcccccgg cgtgagcagg acgttgcaca ggagcgcgac cacctcgtct gacccgttcc
     1441 ccaggatgac ctgatcgggc cgcacatcga ggcgcgcggc gatcgccgcg cgcagggcca
     1501 cgcccgagcc gtccggatac cggtgcaacc cctccaacac ttcacgcacg gccttgagcg
     1561 cggcgggcga tggacccagc gggttctcgt tggacgcgag cttgatggcg tgcgacaccc
     1621 cgtactcgcg ctccacctcg gccacgggtt tgcccggcac gtagggcgcg atgcgggaga
     1681 gttccggtcg aacgcgaaac gccatgggtg atcgactcac gcgatgcggg ggtaggaccc
     1741 gaggaccttg aggacctggc agtggggacg gagcgcgtcc agcgccttct tgaccggctc
     1801 gtcgtccacg tgtccctcga cgtcgatgta gaagtaatac tcccacgcct tgcggcgcga
     1861 cggtcgcgac tcgatcttgg tcaggctcag tccgaattcc gcgaagggtt tgagcatgtc
     1921 gtacagcgcg cccacgcggt cctggaccga caccatgatc gaggtcttgt cccggccggt
     1981 gcgttcgggc gcgcgcggcg cgatcaacag gaaccgggtc gtgttgttcg gattgtcctc
     2041 gatgcgcttc tggatcacgg acaatccgta caaccgggcc gccacctcgg acgcgatcgc
     2101 ggcggcctcg ggctcgtcgc ggcacatttc cgcggcgcgc gccgtgctgg agacctcgac
     2161 gagcgggata ttgggcacgt gttgggccag ccacccccgg cactgggcga tgggctgcgg
     2221 gtgcgagtaa atgcgcttga cgtccgcgag cacgcccgag cgcgacaaga ggtagtgcga
     2281 gatttcctgg acgatctcgc ccgcgatctt gagctgcgaa tccgcgagca ggtccaaggt
     2341 gtgcgtgacc acgccctcgg tggagttctc gatcgggacc acgccgtaat ccgcgcgccc
     2401 gcgctccacc tcgttgaaaa cgtccttgat ggtgttgacc gcgatcgcgg cggccgacgc
     2461 gccgaattgc ttgagactcg ccacgtgagt gaacgtgccc ggcggcccca gatacgccac
     2521 tttcaacggc tgttcgaggg cgagcgacgc ggacatgatc tcgcggaaca ccgcgcgcac
     2581 gccgtcattc ggaaaaggac cggggttctc ccgctccaga cgttcgaaaa tctcctgctc
     2641 ccggcgcggg acg
//
LOCUS       DBNGTT010000472         6219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3178884, whole genome shotgun sequence.
ACCESSION   DBNGTT010000472 DBNGTT010000000
VERSION     DBNGTT010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3178884"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="AC16BC_10740"
     CDS             <1..435
                     /locus_tag="AC16BC_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HIR3242136.1"
                     /translation="DYRPIYPKIDPEAVINPCHPNLTIWHNKIEVGLFMDAHCHFVNV
                     MMKIIRAGTNCYTIALCANDTHEDAIASLGWVDLDKLARLRKAIVSLRNSGKVVPWAL
                     TDAGKAELAERAKRRAEARREVQATMERWDHELATGLDEHAE"
     gene            487..756
                     /locus_tag="AC16BC_10745"
     CDS             487..756
                     /locus_tag="AC16BC_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3242137.1"
                     /translation="MTPATIARPDSASSSPEDVGWLIDWYRRQLAVTPRDIDLRLKCG
                     LFLLDIRRTEEAVAVLQDILRMDPNHLAAREALVQARSLDRGTAS"
     gene            756..2531
                     /locus_tag="AC16BC_10750"
     CDS             756..2531
                     /locus_tag="AC16BC_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3242138.1"
                     /translation="MPEDLLPSTAWPVMSRRLSRLDESLIVRFVGVGGDGVLTAGRIL
                     AEAAAWSGLEVLSGATFSAEVRGGDAMYQLRMSPSALLTEGDRVDVWVLLDRDAHSCP
                     REDLTSGNVVLYEHGHGAAPRDDPPGVVWYPVPMTELAESAGCPVAKNVVALGALAEL
                     FGFSLANIETSLRKRLATKGDRVVASNLAALAGGARYAKEHLVKRDRFLAPVGAAREE
                     TVLVTGNEAIGLGAIMAGCRLYAHYPITPATSLAEWLAAHLPLFGGKICAAEDGIASL
                     GVAIGASVAGVKAMTATSGPGLDLMQELIGYASMAEVPVVIVNVQRAGASTGLPTKYE
                     QADLLPAICGAHGDAPRIVIAPGDVLDCMHLTVEAFNLAERYQVPVILLSDASLALRE
                     HTIARPDLAAITVVDRAPFLPDQGPYSRYRLTDSGISPMSVPGQLGGEYAATGLEHTI
                     SAFPSDAPDVHQAMTDKRFHKLLPIEDDRAPVEREGPPAAAVGLVSWGSTQGAVREAV
                     RRFTAIGMPVAALYPKVLWPPPVKALTAFARSVDQVVVVEGNRQGQFARVIAASTAIK
                     PKLVTRYRGVPLSAWDIFGREDLTH"
     gene            2551..3393
                     /locus_tag="AC16BC_10755"
     CDS             2551..3393
                     /locus_tag="AC16BC_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3242139.1"
                     /translation="MPTIEHDRSTFSTGRDSAWCRGCGHADVFDVLRRALASSGARPA
                     ETVVVTGVGCSSLITFALRSYAIHGAHGRALPLALGVKAANPDLRVVVAGGDGDAFSI
                     GGNHLLHAARKNPRLTYLIMDNQSYGMTRGQPSPTNVRETAHPLNALAVLLAAGATFV
                     AQTATWRPDEAHTVIARALNHPGFSVVNMLSPCPTFHHGEGPTGSNGIPLVKEIGSAH
                     DAGNLTHALSAAFSDGVPCVGLLYRVERPMYALTEGSALSHEPRKDAAILEAVLDEHR
                     VAWT"
     gene            3467..4078
                     /locus_tag="AC16BC_10760"
     CDS             3467..4078
                     /locus_tag="AC16BC_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HIR3242140.1"
                     /translation="MERQQPQSKYRVLPGPEGFLPPAAAAMGVMTPDAGQILVEGAPV
                     PVAEGMEIAAKRLLAAKNPTIFPGPLVLWHWSDRAAAEAKAVKKLAEAIPARVIPMAD
                     YRPKYPKINPEVEINPNHPNITIWHNRIDVCVFVGVHCHQSNIALKIIRGGTNCYTIA
                     LCTFHGDEEANITIRDLTASTILQLADTVKRLKTSSAKRSKPQ"
     gene            4242..>6219
                     /gene="hyfB"
                     /locus_tag="AC16BC_10765"
     CDS             4242..>6219
                     /gene="hyfB"
                     /locus_tag="AC16BC_10765"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HIR3242141.1"
                     /translation="MFDPALIVRPALLSLVTAHVAGALVPLLWPWSDQVKNWLAHGAA
                     VVACAAGVLIGISGLLAPDPLVFSLPSTVPLLHIQLRLDPLSAFFVLVISVSGVAVSI
                     YALGYVREFFGRHSISAMGTLYHGFLLSMILVVTADDGFFFLIVWEIMSLVSYLLVVT
                     EHEQPETRYAGFFYLVMTHVGTAFILIAFLVFYQQTGSFAFDAFRHPGEALPEGFRTI
                     AFVAALIGFGAKAGIVPLHVWLPYAHPAAPSHISALMSGVMIKTAIYGLIRVYFDFLG
                     GAFPWWWGFAVLAIGAVSALLGVMYALMEHDLKSLLAYHSVENIGIILLGLGAGMIFQ
                     SYGLSHLAGLGLLAALYHTMNHAMFKGLLFMGAGSLLYATHTRNMEEYGGLIRKMPWT
                     AFFFLVGAISISALPPTNGFVSEWLTFQSLFLSFMLPDLLMKILIPIGAAMLALTGAL
                     AVACFAKAFGISFLAMARSRHAKDAKEVPIPMRIGMGTLAVVCVALGLAPMVVVPMLD
                     GVIAPFTGAQPANSVIAKAGWALMPMGVGFASISTPVLTILLAVIIPVVFLGVVLIAG
                     RLKRRFYKTWGCGINLSPRMEYTATGFAQPIRQVFSTIYRPTVKIEKDMLEESRYIAK
                     RMRFEVHMEPTFQRYLYDPVVWVFSMVANRLRVVQ"
BASE COUNT         1056 a         2040 c         1934 g         1189 t
ORIGIN      
        1 gactaccggc cgatctatcc caaaatcgac cctgaggcgg tgatcaaccc ctgtcatccc
       61 aacctgacga tctggcacaa caagatcgag gtgggcctgt tcatggacgc gcattgccac
      121 ttcgtgaacg tgatgatgaa aataatccgg gccgggacga actgttacac gatcgcgctg
      181 tgcgccaacg acacccacga ggacgccatc gccagcctcg ggtgggtcga cctcgacaag
      241 ctcgcccgcc tgcgcaaagc cattgtctcg ctgcgtaaca gcgggaaggt cgtcccgtgg
      301 gcgctcacgg acgccgggaa agccgaattg gcggaacgcg ccaaacgccg ggccgaggct
      361 cggcgcgagg ttcaagccac catggaacgg tgggatcacg aattggccac gggtctggac
      421 gaacacgcgg aataggggtt cgacatgtcg aacatctaca tggcggtacg cagagggaat
      481 cgctccgtga cgccggccac aatcgccaga ccggacagcg cgtcgagttc tccggaggac
      541 gtgggctggc tgatcgactg gtatcgccga caactcgcgg tcacgcctcg tgatatcgac
      601 cttcgattga agtgtgggtt gttcctcttg gacatccgcc gcaccgagga ggccgtcgcg
      661 gtgcttcagg acatcctccg gatggatccg aatcacctag ccgcgcgaga agccttggtt
      721 caggcccgct cgcttgatcg cggaaccgcg tcctgatgcc cgaagacctc ctcccgtcca
      781 ccgcgtggcc cgtgatgtcg cgtcgactga gccggctcga cgagagcctg atcgtccgat
      841 tcgtgggcgt gggcggcgac ggggtgttga ccgccggacg aatcctcgct gaagccgccg
      901 cgtggtccgg attggaagtc ctgtccggag ccacgttctc cgcggaagtc cgcgggggag
      961 acgcgatgta tcagctccgg atgtccccct cggccctctt gacggaggga gaccgggtcg
     1021 atgtgtgggt cctgctcgat cgcgacgccc atagctgccc ccgcgaggac ctgacctccg
     1081 ggaacgtcgt gctctacgag catggtcacg gcgcggcgcc ccgcgacgat cctcccggcg
     1141 tcgtgtggta tcccgtgccg atgaccgaac tcgccgaatc cgccggctgt ccggtcgcga
     1201 agaacgtcgt ggcgctggga gcgctcgcgg aattgttcgg gttctcgctc gcgaacatcg
     1261 agacgtcatt gcgaaaacgg ttggcgacca agggggaccg cgtggtggcc tcgaacctcg
     1321 ccgcgttggc cggcggtgcg cggtacgcga aggagcatct ggtcaagcgt gaccgcttct
     1381 tggcgccggt cggcgcggcg cgcgaggaaa cggtcctcgt caccggcaac gaggccatcg
     1441 gcctcggggc gatcatggcc ggctgccggc tgtacgccca ttacccgatc acgccggcga
     1501 cgtcgcttgc ggagtggctc gccgctcacc ttcctctgtt cggcgggaag atctgcgcgg
     1561 cggaggacgg gatcgcgtcg ctcggcgtgg ccattggagc ctccgtggcc ggcgtcaaag
     1621 ccatgaccgc cacatccggc ccgggactcg acttgatgca ggaactgatc gggtacgcct
     1681 ccatggccga ggtcccggtg gtgatcgtca acgtccagcg cgccggagcc agcacggggc
     1741 tgcccaccaa gtacgaacaa gcggacctgc tgccggccat ctgcggagcg cacggcgacg
     1801 cgcccaggat cgtgatcgcg ccgggagacg tcctcgattg catgcacctc accgtcgagg
     1861 cattcaacct cgccgagcga taccaggtcc ccgtcatttt gttgagcgac gcgtcgctgg
     1921 cgcttcggga acacacgatc gcgcggccgg atctcgcggc gatcacggtc gttgatcgcg
     1981 cgccgtttct gcctgaccag gggccctatt cccggtatcg gctgaccgac tccggcattt
     2041 cgcccatgtc cgtcccagga cagctcggcg gtgaatacgc ggcgaccgga ctcgaacata
     2101 cgatatcggc gtttccctcg gacgctcccg acgtccacca agccatgacg gataagcggt
     2161 ttcacaaact cctcccgatc gaggacgacc gcgccccggt tgagcgcgag ggtcctcccg
     2221 cggcggccgt gggcctggtc agttggggat cgacccaggg cgcggtgcgg gaggcggtcc
     2281 ggcgcttcac ggccatcggc atgcccgtcg cggcgctgta tccgaaggtc ttgtggccgc
     2341 ctccggtcaa ggcgctgacg gcattcgcgc ggtcggtcga ccaggtcgtc gtcgtggaag
     2401 gcaatcgcca aggccagttc gcgcgcgtga tcgccgcctc gaccgcgatc aaaccgaaac
     2461 tggtgacccg gtatcgcggc gtgccgttga gcgcgtggga tattttcggg agagaagacc
     2521 ttacccactg accgacgcgc tggagattcg atgccgacca ttgagcacga tcgttcgacg
     2581 ttttcgaccg gaagggactc cgcctggtgc cgaggctgcg gacacgccga cgtgttcgac
     2641 gtcctgcgcc gcgcgctggc gtcttccggc gcgcgaccgg ccgagacggt cgtcgtcacc
     2701 ggagtgggct gttccagcct gatcacgttt gcgcttcgga gctacgcgat tcacggcgcg
     2761 cacggtcgcg cgctcccatt ggccctcggc gtgaaggcgg caaacccgga cctccgcgtg
     2821 gtggtggcgg gcggagacgg cgacgccttc tcgatcgggg ggaatcatct cctccacgcc
     2881 gcccgcaaga atcccagact cacctatctg attatggaca accaatccta cggcatgacg
     2941 agaggacaac cgtctccgac gaacgtccgc gagaccgccc accccttaaa tgccctcgcc
     3001 gtcctgctcg cggccggcgc gacgttcgtc gctcagacgg cgacgtggcg gccggatgaa
     3061 gcgcacacgg tgatcgcgcg ggccctcaac catccgggat tttcggtggt gaatatgctc
     3121 tcgccctgcc ccacgtttca ccacggagag ggaccaaccg gatcgaacgg catcccgctc
     3181 gtgaaggaaa tcggatcagc ccacgacgcc gggaacctga cccacgccct gtcggccgcc
     3241 ttctcggacg gcgtgccgtg cgtcggcctc ctctaccggg tggagcgccc catgtacgcg
     3301 ttgacggaag gttccgctct ctcccacgaa ccacggaaag acgcggcgat ccttgaagcc
     3361 gtgttggatg aacaccgcgt ggcatggacg tgaccaaccg gcatcgccga ccgccgacac
     3421 ggtcgtatcc ctgaacatct tcacaaccga aaggaggacg tcacacatgg aacggcaaca
     3481 gccacaatcg aaataccggg tgcttccagg tcccgaggga tttctccccc ccgcagcggc
     3541 cgcgatgggc gtgatgaccc ccgacgcggg ccagatcttg gtcgaggggg cgccggttcc
     3601 ggtggcggag ggcatggaga tcgccgccaa gcggttgctc gccgcgaaga atcccacgat
     3661 ctttccgggt ccgctcgtcc tctggcattg gtcggaccga gcggcggcgg aggccaaggc
     3721 cgtgaagaag ttggcggaag cgattcccgc tcgcgtcatt cccatggccg actatcgtcc
     3781 gaaatacccc aagatcaacc ctgaggtcga aatcaacccc aaccacccca acatcacgat
     3841 ttggcacaac cgcatcgacg tgtgcgtctt cgtcggcgta cactgccacc agtcgaacat
     3901 cgcgctgaaa atcatccgcg gcggaaccaa ttgctatacc attgcgctct gtacgttcca
     3961 cggcgatgaa gaagccaata tcacgatccg cgacctgacc gcctcgacga tcctgcagct
     4021 cgccgatacc gtcaagcgac tcaagacctc atccgcgaag cgatcgaaac cgcaataggg
     4081 tcacgcgcct gcctgagtcc cacgacacga ctcattcacg accattgtgt tctccgcgca
     4141 ctttgtccta tgatgcgagg cagttcgtgt caggcactct cctgcgcgcg tcaacgcgtc
     4201 gtacacggtc ataacgaggg acggcagcgt gtggcacttt cgtgtttgat cctgcgttaa
     4261 tcgttcgtcc ggccctgctc agcctcgtga ccgcgcatgt tgccggcgcg ctggtccccc
     4321 tgctctggcc gtggagcgac caggttaaga actggctggc ccacggcgca gcggtcgtgg
     4381 cctgcgcagc aggcgtgctc atcggcatca gcgggttgct ggctcctgac cccctggtct
     4441 tctcgctgcc ttcgaccgtt ccccttctgc acatccaact ccgactcgac cccctgtcgg
     4501 cgttttttgt gctcgtcatc tccgtctcgg gcgtcgccgt ctcgatctac gccctcgggt
     4561 acgtgcggga gttcttcggg cgccactcga tcagcgccat gggcacgctg taccacggct
     4621 tcctgctctc gatgatcctc gtggtgacgg cggacgacgg cttcttcttc ctcatcgtct
     4681 gggagatcat gtcgctcgtt tcgtacctgc tcgtggtcac cgagcacgag cagccggaga
     4741 ccaggtacgc ggggtttttc tatctcgtga tgactcacgt gggaacggca ttcatcctca
     4801 tcgcctttct ggtgttttac caacagaccg gtagttttgc cttcgacgcg ttccgccatc
     4861 ccggcgaagc gcttcccgaa ggctttcgga ccattgcctt cgtggcggcc ctgatcggat
     4921 tcggggccaa ggccggaatc gttcccttgc acgtctggtt gccctacgcg caccccgctg
     4981 ccccgtccca tatttctgcc ctgatgtcgg gcgtcatgat taagaccgcg atttacggat
     5041 tgatcagggt gtatttcgac ttcctcggcg gtgcgttccc ctggtggtgg ggctttgccg
     5101 tgctggcaat cggagcggtt tcggcgttgc tgggcgtcat gtacgccctg atggaacacg
     5161 atctcaagag cctgctcgcc taccacagcg tggaaaatat cggcatcatt ctcttagggc
     5221 tcggcgccgg catgatcttc cagtcgtacg gcctttccca tctcgccggg ctcgggttgc
     5281 tggccgcgct gtaccacacc atgaaccacg ccatgttcaa gggcctcttg ttcatgggag
     5341 cggggtcgtt gctctacgcg acccacacga gaaacatgga agaatacggc gggctgatcc
     5401 gcaagatgcc gtggaccgcc ttcttctttc tggtgggcgc gatttcgatc tcggcgctcc
     5461 ctccaaccaa cggattcgtc agcgagtggt tgacgttcca gagcctcttt ctcagtttca
     5521 tgctccccga cctcttgatg aagatcttga tcccgatcgg cgcggccatg ctggcgctaa
     5581 ccggggcgtt ggctgtggcc tgctttgcca aggcgttcgg gatttcgttc ttggctatgg
     5641 cgcgcagccg gcacgccaaa gacgccaaag aggttccgat tcccatgcgc atcgggatgg
     5701 gaacgctcgc cgtcgtgtgc gtagccctcg gtctcgctcc gatggtggtc gtcccgatgc
     5761 tcgacggagt gatcgctcca ttcacgggcg cccagcccgc taacagcgtg atcgcgaaag
     5821 cgggctgggc gctcatgccg atgggggtcg gatttgccag catctccacg cccgtcctga
     5881 cgatcctgct tgcggtcatc attcccgtcg tgttcctggg cgtggtgctc atcgcgggaa
     5941 ggctgaaacg ccgcttctat aagacgtggg gctgcggcat caatctcagc ccgcggatgg
     6001 agtacacggc cactggattt gcccagccga tccgccaggt cttcagcacg atctaccggc
     6061 cgaccgtcaa aatcgagaag gacatgctgg aggagtcccg gtatatcgcc aaacggatgc
     6121 ggttcgaggt ccatatggaa ccgacgtttc aacgatatct ctacgatccc gtggtgtggg
     6181 tgttcagcat ggtggccaac cgtctccgcg tcgtgcagg
//
LOCUS       DBNGTT010000473         3850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_241320, whole genome shotgun sequence.
ACCESSION   DBNGTT010000473 DBNGTT010000000
VERSION     DBNGTT010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_241320"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="AC16BC_10770"
     CDS             <1..258
                     /locus_tag="AC16BC_10770"
                     /inference="COORDINATES: protein motif:HMM:NF022666.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HIR3242142.1"
                     /translation="ESRQEQFVQSNFTSLAKDMAAGEGENLAAFAELLGCGAENEKAF
                     GALMQSRYAEIVGDDHAPSAVVAGVKQALAAEPELASRCAL"
     gene            359..2293
                     /locus_tag="AC16BC_10775"
     CDS             359..2293
                     /locus_tag="AC16BC_10775"
                     /inference="COORDINATES: protein motif:HMM:NF051510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lnb N-terminal periplasmic domain-containing
                     protein"
                     /protein_id="HIR3242143.1"
                     /translation="MKGSIHTVGRRVGVLLALMLFGVSTGFAQAFDAPDYSRELIQRA
                     RAIRLFDEREWHLLVHYKRTLFGAYESQEDAPAFFNAPNGKTNPEAELAATIERFFID
                     PTELKPGEAHPQCDFPARYKWLKFRLSFDPTRLREQPCERLDRWMTDINPQGVTLVFA
                     SYFMSSPASMFGHTLLRLDQALGRADGRLLDYSVNFAAAPDTNNPVLYVFRGLFGYFK
                     GQFSVAPYYTRVQDYSNWESRDLWEYPLNLSPDHIDTLLRHLWELGGNSFDYLYFQEN
                     CSYHILSVLEVANPELRLTDRFAFHVIPAETVKAVTEQPGLAGNARYRPSLVSQLNGK
                     RVRLSPDETEGLRALVFNRQPRLGTEYQALTDQAKSRVLDAYLDYRHYLDMQASKRPD
                     WISSETQIMLTERSTLPVVERPGKAVEFSSPPEMGHDSARVRLAGGVDASGIFQEIAF
                     RPAYHDLMAKDDGYSPDSQILFGDVGARYYDRSGRLRLQHFRLIDVVSLSPYETLFHR
                     KSFRFGVGIETIRDLDCLFCNAVRLDYGVGVAGRSGLRGPIRIYSLVDFSGESSHHFD
                     KSYRLGTDLLGGMLADIGDRWRFHITGGYAMFPVGHRSAYSHFAVSQRYAISRNLDVR
                     LDLNRVGARREATFGGSVYF"
     gene            2358..3350
                     /locus_tag="AC16BC_10780"
     CDS             2358..3350
                     /locus_tag="AC16BC_10780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3242144.1"
                     /translation="MSGKFFERRRSVGRVGWSSDGWSARNVWGSLVLSAVAAATVSGC
                     DGLFYFPSRELFHDPAAIGLPFRDVWFESRDGTRLHGWFFPADRQGRASVVGTVVHFH
                     GNAENISAHAYSSAWLSREGFNVLVFDYRGYGESAGTPDKDGVQQDAIAAVRWVSEQP
                     EVDPRRLVLFGQSLGAAIAVRVAADYRPSGLRCLVLESGFSSYRSIARDKLSQSVVLW
                     PFQWPLSFLVSDRYSPARAIAKIAPAPVLIVHGTADPIVPYSQAEALYAAAHQPKTLW
                     TIPGGGHISAFTRNGAVYREPLVAFLRRCVAETPEPCGDDERQRCSGVERSVAR"
     gene            complement(3424..3738)
                     /locus_tag="AC16BC_10785"
     CDS             complement(3424..3738)
                     /locus_tag="AC16BC_10785"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HIR3242145.1"
                     /translation="MRPAWASRNWARDAGCAGVVCSGDEVRAIKEQFGPEFLVVTPGI
                     RPAWAAVPGDDQRRAVTPADAIAAGADFLVVGRPIRAAQDSTEAAEQIVNEIEDALAR
                     QR"
BASE COUNT          690 a         1109 c         1209 g          842 t
ORIGIN      
        1 gagtcccgtc aggagcagtt tgtccaaagc aacttcacca gcttggcgaa ggatatggct
       61 gcgggtgaag gggagaacct ggccgcgttt gccgaactcc tgggctgcgg ggccgagaat
      121 gaaaaggcat tcggcgccct gatgcaaagc cgttacgcgg agatcgtggg cgacgatcat
      181 gcgccgtccg ccgtggtcgc gggcgttaag caggcgctgg cggccgagcc cgaattggcg
      241 tcgcgttgtg ccttgtaagt gatctgaccg cataccactg atacgggggg ataccatctt
      301 gaagcatcct ggtggtatcc ccctttgttt gacatgacgg cataaatcgc ctcgcgacat
      361 gaagggatcg attcacacgg taggacgacg ggtcggcgtc ctactcgcgc tcatgctgtt
      421 cggcgtatcc accgggttcg cgcaggcgtt tgatgccccg gactacagcc gagagttgat
      481 ccagcgcgcg cgcgcgatac ggctcttcga cgagcgcgag tggcatcttc tcgtccatta
      541 caagcggact ctgttcgggg cgtacgaaag ccaagaggac gcaccggcat ttttcaacgc
      601 ccccaacggc aaaaccaatc ctgaagccga actcgcggcg acgattgagc ggtttttcat
      661 cgaccccacc gaactcaagc ccggggaggc gcaccctcag tgcgatttcc cggcccgata
      721 taagtggctg aagtttcgcc tgtcgttcga tcccacccga ctgcgggagc aaccatgcga
      781 gcggctggat cgttggatga cggacatcaa tccccagggc gtcacgttgg tcttcgcgtc
      841 gtactttatg agcagcccgg cgtcgatgtt cggacacacg ctcctgcgct tagatcaggc
      901 ccttggccga gcggacggcc gattgctgga ttacagcgtc aattttgcgg cggctcccga
      961 cacgaacaat ccagttctgt atgtgttcag gggattgttc ggatacttca aggggcagtt
     1021 ctccgttgct ccgtattata cgagggttca ggattactcc aattgggaga gccgcgatct
     1081 gtgggaatat ccgctgaacc tgtcaccgga ccacatcgac acgttgctcc ggcacttgtg
     1141 ggagttggga gggaattcct ttgattacct ctattttcag gagaactgct cgtatcacat
     1201 tctgtcggtc ctggaagtcg cgaacccgga gcttcgactg accgaccggt ttgcgttcca
     1261 cgtgattccg gccgagacgg tcaaggccgt gaccgagcag cccggattgg ccggcaacgc
     1321 ccggtatcgc ccctcgctcg tcagtcaatt gaatgggaag cgcgttcgtc tgtcgccgga
     1381 tgagactgaa gggttgcgag ccctcgtctt taaccgccaa ccgcgtcttg ggacggaata
     1441 ccaggcgctc acggatcagg cgaaatcgcg cgtgctcgac gcgtatctcg actatcggca
     1501 ttatctggat atgcaagcga gtaaacgccc ggattggatc agctccgaaa cgcaaatcat
     1561 gctgaccgag cgcagcacat tgccggttgt cgagcgtccc ggtaaggccg tcgagttttc
     1621 ctcgccgccc gaaatgggtc atgatagcgc gagggttcgg ctcgcggggg gagttgacgc
     1681 cagcggtatc tttcaggaaa tcgcatttcg ccccgcgtac catgacctga tggccaagga
     1741 cgacgggtac agccccgatt cgcagatact gttcggcgac gtgggggctc gctattacga
     1801 ccggtccggg cgtctacggc tccagcattt ccggctgatc gatgtcgtct cgttgtcgcc
     1861 gtatgagacg ctgtttcacc gcaagtcgtt tcgatttggg gtggggatcg agaccatccg
     1921 agacctcgac tgcctgttct gcaacgcggt gcgcctcgat tatggcgtgg gcgtggcagg
     1981 gcggagcgga ctccgcgggc cgattcgaat ctattcgctc gtggacttct ccggagagtc
     2041 gtcgcaccat ttcgataaaa gctaccgact cggcaccgac ctgctcggtg gtatgttggc
     2101 cgacatcgga gaccgctggc ggttccatat cacaggtggg tatgcgatgt tcccagtcgg
     2161 gcatcgatcc gcatactcac attttgcagt gtcccagcga tatgcgattt cccgaaatct
     2221 cgatgtacgg ctggatctta accgagttgg agcccgtcgg gaggctacat tcggcggcag
     2281 cgtctatttt tgagcggagc acctgccgcg ccggagcgtg cggcaaccga actcccgatc
     2341 gagttgtcga ggtgaatatg agcggtaagt ttttcgagag aagacgttct gtcggtcgtg
     2401 tcggatggtc atccgatggc tggagtgcgc gaaacgtttg gggaagcctc gtgttgtcgg
     2461 cggtcgccgc cgctacggtt tccggctgtg acggactttt ctatttccct tcgcgggaat
     2521 tgtttcatga ccccgcggcg atcggtttgc catttcgaga cgtgtggttc gagagccgag
     2581 acggtacccg gcttcatggg tggtttttcc cggcagaccg tcaggggcgg gcgtccgtcg
     2641 tggggactgt cgttcatttt catggcaacg ccgagaatat ctcagcccac gcttattctt
     2701 cggcgtggtt gtcccgtgag ggtttcaatg tgttggtgtt cgactatcgt ggttacggag
     2761 aatccgcggg aacgcctgac aaagacggag tccagcagga cgcgatcgcc gcggtccgtt
     2821 gggtgagcga acagcctgaa gttgatcctc gtcggttagt gctgttcggc caaagcctcg
     2881 gcgcggccat cgcggtgcgg gtcgccgccg actaccgtcc ctcgggcctg cggtgtctcg
     2941 tgctcgaaag cgggttctcc agttaccgct ccatcgcccg agataagttg tcgcagagtg
     3001 tggtgctctg gccatttcag tggccgctct cgttcctggt gtccgaccgg tatagtccgg
     3061 cgagagcgat tgcgaagatc gcgccggctc cggtcttgat cgtccacggc acggccgacc
     3121 caatcgtccc ctattctcag gcggaggcgc tgtatgcggc cgcccaccaa cccaagaccc
     3181 tatggaccat tcctggcggg ggccatatca gcgcctttac gcgcaacggg gctgtctatc
     3241 gggagccctt ggtggcgttt ctcaggcggt gcgtggctga gacgcctgaa ccgtgtggtg
     3301 atgatgagcg acagcggtgc tcgggcgtgg aacggtcggt tgcacggtga gaccgtttcg
     3361 cggatagctg tagtagcaaa tagcattagc tccaaggggc cggcttgaag ttggacgctg
     3421 tagtcaacgc tgccgcgcga gcgcgtcttc gatctcgttt acaatttgtt ccgccgcttc
     3481 ggtcgagtcc tgcgccgcgc gaatcggccg acccaccacg agaaagtccg cgccggccgc
     3541 aatagcgtcg gcgggcgtga cagcgcggcg ttggtcgtcc cctgggaccg cggcccaggc
     3601 aggacggatg ccgggggtga caacgaggaa ctcggggccg aattgctcct tgatcgcgcg
     3661 aacctcgtct ccggaacaga ccacgccggc gcagcccgcg tcgcgggccc aatttctcga
     3721 cgcccaggcc gggcgcatgg tcgattgcct ctcagatccc gcgcacggcc attatcttga
     3781 atttactcga tgtattgagt aaatttactc atcatattga gtaaattgtt catggcctac
     3841 acgcgtcccc
//
LOCUS       DBNGTT010000474         3567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3051782, whole genome shotgun sequence.
ACCESSION   DBNGTT010000474 DBNGTT010000000
VERSION     DBNGTT010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3051782"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC16BC_10790"
     CDS             <1..509
                     /locus_tag="AC16BC_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3242146.1"
                     /translation="KGVFLIAEPSLQDPNFSETVILITHHSPRGSTGVVINRPTTTLL
                     SRVLPDRKELAGRLDVLHAGGPVDPRVFLLLVRSRTRPAHAQHVFGDVYVTQAADALL
                     DALKSDDETTVIRLFAGYAGWAPGQLADEIARGSWRVVPADAAVLFDRESEVIWKEMI
                     RRTSDQFI"
     gene            complement(622..987)
                     /locus_tag="AC16BC_10795"
     CDS             complement(622..987)
                     /locus_tag="AC16BC_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3242147.1"
                     /translation="MPIYEYQCQKCRHREELIQKVSDPPLTECPKCKGPMTKLIAAPA
                     LQFKGSGFYITDYSDKGKDKSDKSKSKSDSGSTEKTGGAARPAAESTSSTSAASTDKK
                     TDAGGSSSPPAKPSGDGKS"
     gene            complement(998..2191)
                     /locus_tag="AC16BC_10800"
     CDS             complement(998..2191)
                     /locus_tag="AC16BC_10800"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3242148.1"
                     /translation="MKLAQRVGRIQPSPTLAMAARAKALAAQGIDVIDFGVGEPDFDT
                     PDAIKDAAIAAIRAGFTKYTPPSGTEELKRAIIARLETDLGLRYQLNEVVVSCGAKHT
                     LYNLAQALFEAGDEVIIPAPYWVSYPDQVLLNDATPVFVQTMAAEGFILDPKRLEAAV
                     TPRSKALILNSPSNPTGAGYTKAQLERIAEVALRHRLIVISDEIYGQIVYDGYRHVSI
                     ASLSPEMKARTVVVDGVSKTYAMTGWRIGYAAGPAALMTAVGTMQSQSTSNPTSISQK
                     AAVAALTGPRASVERMVTEFSARRTEMVQRLSAIPGVTCFRPQGAFYAFPHVGSYYGK
                     RAGATTIKDSNDLATYLLDEGRIAVVAGAGFGADGHVRLSYATSTEKIKTGLDRMKAA
                     LAKLQ"
     gene            complement(2188..2676)
                     /gene="coaD"
                     /locus_tag="AC16BC_10805"
     CDS             complement(2188..2676)
                     /gene="coaD"
                     /locus_tag="AC16BC_10805"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR3242149.1"
                     /translation="MTRIGVCPGTFDPITNGHLDIVRRSLHFFDAVILAVALNPSKTP
                     LFDIKERMEMAVDATRGIERVTVEAFDGLLVDYLRQKGAHGIIRGLRAVSDFEYELQM
                     AMMNRNLDQNVETVFLMPSVEYSFLTSTIIKNVASYGGNVDDLVPAVAARRLREKFGG
                     RR"
     gene            complement(2673..3242)
                     /gene="rsmD"
                     /locus_tag="AC16BC_10810"
     CDS             complement(2673..3242)
                     /gene="rsmD"
                     /locus_tag="AC16BC_10810"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR3242150.1"
                     /translation="MRVIAGRFKGRRLRAPRGFTVRPTSDKVKGALFNILGSRIQGAR
                     VMDLFAGTGSIGIEALSRGAAHVLFVEDDPAAREALKANIESCEIPSREATLWKGRGV
                     AGLIRHLKTRPGETADLIFADPPYRQPETFRLLRPFVAGLGLADDGWLVVEHVRSAEL
                     PKPEPPVALTRTCVYGDTVLSIYTREEQP"
     gene            complement(3381..>3567)
                     /locus_tag="AC16BC_10815"
     CDS             complement(3381..>3567)
                     /locus_tag="AC16BC_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR3242151.1"
                     /translation="RDGLLWAGTDGGASWYDGSRWRTYTTRDGLMDNNVFSMAFDDHG
                     ARWFGTWKGLSKLEITR"
BASE COUNT          587 a         1131 c         1160 g          689 t
ORIGIN      
        1 aaaagggggt gttcctcatc gcggagccga gcctgcagga tcccaatttc agcgagaccg
       61 tgatcctcat cacgcaccac agtccgcgag gcagcaccgg ggtggtgatc aaccgtccga
      121 ccaccacgct gctttcgcgg gtgttgcccg accgaaagga acttgccggg cggttggatg
      181 tgttgcacgc gggcggaccg gttgatcccc gggtattcct gctgttggtg cgctcgcgaa
      241 cgcggcccgc gcacgcgcag cacgtgttcg gcgacgtcta cgtcacgcaa gccgctgacg
      301 ccttgctcga cgcgctgaag agcgatgacg agacgaccgt gatccgtttg ttcgcgggat
      361 acgcggggtg ggcgcctggt caactggcag acgaaatcgc gcgcggcagt tggcgcgtgg
      421 ttccggccga tgccgcggtg ctgttcgacc gcgaatcaga ggtgatctgg aaggagatga
      481 tccggcggac gtcggatcag tttatctgac gggtacaatg gggccctggt cggcaggccc
      541 gtgagagacg tggtgcgaca ggctcaccac gaacgggaac ttaggcttct ccgttcgtcc
      601 tgagcttgtc gaaggaccgt actagctctt cccgtcgccc gatggcttgg ccggaggaga
      661 tgatgatccc ccggcgtcgg tctttttgtc ggtcgaggcc gcgctggtag aggacgtgga
      721 ttcggccgct ggtctggccg cgccgcccgt tttctcggtt gatccgctgt cggatttgct
      781 tttacttttg tccgacttgt ctttgccctt gtccgaatag tccgtgatgt agaagccgga
      841 acctttgaat tgaagcgccg gcgccgcgat gagtttggtc atgggcccct tgcacttggg
      901 gcattcggtc aacggcgggt cgctgacttt ttggatcagt tcctcgcggt gacggcactt
      961 ttggcattgg tactcgtaaa tgggcaccac aaacctccta ttgcagcttg gccaacgcgg
     1021 ccttcatacg atccaatccg gttttgatct tctccgtcga cgtggcgtag gacaaccgga
     1081 cgtggccgtc cgcgccgaag cccgcgcccg ccaccaccgc gatgcggcct tcgtcgagca
     1141 ggtaggtggc caggtcgttg gagtctttga tcgtggtcgc gcccgcgcgc ttgccgtaat
     1201 acgaccccac gtggggaaac gcgtagaacg cgccctgggg gcgaaagcag gtcacgccgg
     1261 ggatggccga caaccgttgg accatctcgg tgcgccgcgc cgagaactcg gtgaccatgc
     1321 gttccacgga cgcgcgcggg ccggtgagcg cggccaccgc cgctttttgc gagatcgacg
     1381 tcggattgga ggtgctctgg ctctgcatgg tgcccaccgc ggtcatcaac gcggccgggc
     1441 ccgcggcgta accgatgcgc cacccggtca tggcgtacgt cttggacacg ccgtccacca
     1501 ccacggtgcg ggccttcatc tccggggaga gcgacgcgat gctgacgtgc cggtatccgt
     1561 cgtacacgat ctggccgtag atctcgtcgg agatcacgat cagccggtgg cgcaacgcca
     1621 cctcggcgat tcgctcaagt tgcgctttgg tgtagcccgc gccagtggga ttggacggcg
     1681 agttcagaat cagcgccttg ctccgcggag tgaccgcggc ttccaatcgt ttcgggtcca
     1741 gaatgaaccc ctccgcggcc atggtttgga cgaacacggg cgtggcgtcg ttgagcagca
     1801 cttgatccgg atacgacacc cagtagggcg ccgggatgat cacctcgtcg cccgcttcaa
     1861 agagcgcctg cgccaggttg tagagcgtgt gtttggcgcc gcacgagacc acgacttcgt
     1921 tgagttgata ccgcaagccc aagtccgtct ccaagcgcgc gatgatggcc cgcttgagtt
     1981 cctcggtccc ggacggcggc gtgtatttgg tgaacccggc cctgatcgcg gcgatggccg
     2041 cgtccttgat cgcgtccggg gtgtcgaagt cgggctcgcc cacgccgaag tcgatgacgt
     2101 cgatgccttg cgcggccagg gccttggcgc gggcggccat cgcgagtgtc ggggacggtt
     2161 ggatgcgtcc cacgcgttgc gcgagcttca tcggcggccc ccgaacttct cgcgcaagcg
     2221 gcgcgcggcc acggccggca ccaagtcatc cacgttcccg ccgtagctgg cgacgttctt
     2281 gataatggtc gaggtcagaa acgagtattc cacgctgggc atcagaaaca cggtctccac
     2341 gttttgatcc agattgcggt tcatcatcgc catctgcaac tcgtactcga agtcggacac
     2401 ggcgcgcagg ccgcggatga tcccgtgcgc gcccttttgc cgcaagtaat cgaccaacag
     2461 cccgtcgaac gcctcgaccg tcacgcgttc gatcccgcgc gtggcgtcaa cggccatctc
     2521 catgcgctcc ttgatgtcga agagcggcgt cttcgagggg ttgagcgcca ccgcgaggat
     2581 cacggcatcg aagaagtgca ggctccgccg gacgatgtcc aggtggccgt tggtgatcgg
     2641 gtcgaacgtg cccgggcaca cgccgatgcg cgtcacggct gctcctcccg cgtgtagatc
     2701 gacaacacgg tgtcgccgta gacgcaggtg cgggtcagcg cgaccggcgg ttcgggcttc
     2761 gggagttccg cgctgcgcac gtgctccacg accagccagc cgtcatcggc caaccccagg
     2821 cccgcgacaa acggacggag caaccgaaac gtttcgggtt gtcgatacgg cgggtccgcg
     2881 aaaatcaggt ccgcggtttc tccggggcgc gtcttgaggt gccggatcag gccggccacg
     2941 cctcggcctt tccagagcgt ggcttcgcgc gacggaatct cgcaggactc gatattcgcc
     3001 ttgagggcct cgcgtgccgc ggggtcgtct tccacgaaca atacgtgcgc cgcgccgcgg
     3061 ctcaacgctt cgatgccaat gctcccggtg ccggcgaaca ggtccatgac ccttgcgcct
     3121 tggattcgtg atcccaggat attgaacaac gcgcccttga ccttgtccga cgtggggcgc
     3181 accgtgaacc ctcgcggcgc ccgcaagcgg cgtcctttga atcgtccagc gatgacgcgc
     3241 atcagcttgc tcggccccgc aattcagccg agtaccttaa catgcgctca aaacagcggt
     3301 caacattcgg caagagatcc cggcgctttc tattcgccat tcccgcaaag gcgggaatcc
     3361 agagcgtggc tcttgaggcg ctaacgagtg atctccagct tgctgagccc tttccaggtg
     3421 ccgaaccatc gcgcgccgtg gtcatcgaac gccatggaga agacgttgtt gtccatgagc
     3481 ccgtcgcggg tggtgtacgt gcgccagcgt gagccgtcat accaactggc gcctccgtcg
     3541 gtgccggccc acagcaggcc gtcgcga
//
LOCUS       DBNGTT010000475        11363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3434156, whole genome shotgun sequence.
ACCESSION   DBNGTT010000475 DBNGTT010000000
VERSION     DBNGTT010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11363
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3434156"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2288)
                     /locus_tag="AC16BC_10820"
     CDS             complement(<1..2288)
                     /locus_tag="AC16BC_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242152.1"
                     /translation="MSGRVDAWWLPRSGARVLAVTIGAAALAAIATVSAWPRLAAAPQ
                     PYSDLVAGMITWSDYPKSSEYVAAAWFFAAFVFAWPILSRLANQLPWPERGAVMIETP
                     AVPYALYLGIATYAAAFGLVTRRWPWFPLAAALVLPAAWAGLTRGSTVIPAIVPAGPA
                     FVALLVAVVCAYFSLLGVLAAMDAVIPTWFAGHTSLWSAFALTTPFIFACGLVLGHRR
                     FSPATWRAIASGSQIPLPLLITVLAHDAYLHDGLVTRLNMPTMTKAGVLVFAAAMCAR
                     EMIAWRRAEPWLVVPDANRMIRPSSVVWIAAFLAYRVPEFSGFEWDSLHLGELLLPWH
                     QVVTHDRALFDQFVPAQGGMSLLYGAVNTLALDGAVSSFPIAVSWLSVAAAAGAAWTV
                     CRLGGPGWALLLAPFLPTGYTSVLASFNRFFLALPILAVLAHPTLLARPRRWLMAWAG
                     LSMVHVLYNAIVGLACTAATAPVAAWMAVRARTPQAEIAPSMITPRRTAMMFAIGGLG
                     AALAPSLGAYLLGLARFVIENAPTNTTAFGVGVLQAGRVPDWFWWHDNKLAWELIRIG
                     GWVGGAIALVALAVHQVSRQRRGDPVEPAGVVITLTGVLFLLALIPYSLGQVWPDELS
                     RSGAASMLAIGGLLPLAIILTRRPDPRAALIAAGVIGALLGVRGAFGDPNPWQIAQRA
                     IAAIDAPARTVQRSGAAAGVPNAGEGFVLPDKLDAIQRLNAALRRLLRDGETYLDLTN
                     RPALYFVLDRPVPAPYPDDYNAP"
     gene            complement(2285..3100)
                     /locus_tag="AC16BC_10825"
     CDS             complement(2285..3100)
                     /locus_tag="AC16BC_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3242153.1"
                     /translation="MNLAVSNIAWPRDQEHAAAALLETRGVRGVEIAPSAVWSDPTAS
                     SKRERLEYRRAWERRGIAIVAFQALLYGRPELAIFSGPDARAATMDYLFRLIHLAVDL
                     GARVLVFGSPGHRRTAGLAPREADEIAETFFRGVGRVGEHYGVQLCIEPIPRSWGCEF
                     VTTIREGLDLVRRVDSAGFGLHLDAASAAAEPDAASVIREAGTVVAHVHASEPNLAPI
                     GSGGVNHAAFAAALRRIGYSSWVSIEMRVADRERSIDRVDEALRHARTAYGLT"
     gene            complement(3097..3867)
                     /locus_tag="AC16BC_10830"
     CDS             complement(3097..3867)
                     /locus_tag="AC16BC_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3242154.1"
                     /translation="MAVSLIGDTGFVGGNLLRQVPVDATYNSGTIDAIVGRCFDLLII
                     AGAPGAKWRANQDPVADAAAIARLTAALMQVTARRAVLISTVDVYPNAVGVSEATTVV
                     PTDASPYGRHRRQLEDVVTARFDAVVIRLPGLFGPGLRKNVIFDLLNRRGIEQINPDS
                     TYQWYPIDRLWIDIRLALAKRLRLVNLVTEPLRVDELARDAFGLELAAAGASAPATHY
                     DVRTEYDRLFGGRSGYLAAKTESVRRITAFVKQARAQV"
     gene            complement(3852..4652)
                     /locus_tag="AC16BC_10835"
     CDS             complement(3852..4652)
                     /locus_tag="AC16BC_10835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3242155.1"
                     /translation="MSAPRVAVLGPRASRYCLCIPVINEGERLHAQLYRMRDIAGSVD
                     VVIADGGSTDGSVALPALREFGVRAVIPKSEPGALSTQLRLAFAYALEAGYDGVATMD
                     GNNKDDPASIPLVLRALDLGYDHVQGSRFVPGGRAVATPWTRLVAIRLLHAPVISLAA
                     GVRYTDTTNGFRGYSRRFLLDPRVAPFRPVFRGYELPYYLAAQAGRLGYRVIEVPVTR
                     RYPPGRVPTKIRPLVGHLEILSALWAVWRGRYAPPISPDERETRWPSA"
     gene            complement(4694..5698)
                     /locus_tag="AC16BC_10840"
     CDS             complement(4694..5698)
                     /locus_tag="AC16BC_10840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3242156.1"
                     /translation="MSVGLEPVVSVVAPLRNNAGIITEFLAETHGVLRRHFRHYEIVL
                     VDDASTDGTVAAAVSVFDRYDHVRVVTLSRAFGAEIAITAGLDLAIGDVVVVMSPDSD
                     PPELIPTMVRRCAAGSGVVFGVRSHRAGESLLVRIGAALFYWLCRRVFGLALPSNATH
                     FRALSRQAVHAIGQIKDRRRSLWVSSAYIGYGTDSLTYHPQSRSGNRNVTRFFQGIGL
                     AFQVISGNTTAPLRLAGRAALLGGVVSFLALGYVAGTWLFTRHVAEGWATLSSLAASM
                     FATVLAVLGVVCEYLARLLDESWNRPLYYIRDERTSPLLLAAVPHRNVLVDPPQTVRL
                     "
     gene            complement(5695..6888)
                     /locus_tag="AC16BC_10845"
     CDS             complement(5695..6888)
                     /locus_tag="AC16BC_10845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3242157.1"
                     /translation="MAPHGADAEDIARRDADVSSSDAWDAAIIGGGFFGCRLALELKR
                     QGLERVVLVEREADILRRASFANQARVHHGYHYPRSFLTALRSRVNFPKFVEEYPDCI
                     DRGFEHYYAVSTINSLAGISASRFRTFCDRISAPIEPAPAEVRRLFNPGLIEDVFLVQ
                     EYAFDAVRLRRALRAQIDRSGVSLALNTDVTAVRRGRDGRMELTLERGGRSGALLAER
                     VLNCTFARANRLLRNSGLPTLPLRYELAELALVEPPDALRRRAITVVCGPFFSIMPFP
                     ARGLHSLSHVRYTPHVAWTDGPDAPPDGPPSPARLGRASRYTHMVADAARFVPVLREC
                     RHVDSLWEIKTALPSSKSDDSRPIVIASTPGLPNFHSIVGAKIDQVYDLLSELDLMAA
                     SGGRA"
     gene            7119..7304
                     /locus_tag="AC16BC_10850"
     CDS             7119..7304
                     /locus_tag="AC16BC_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242158.1"
                     /translation="MKRFVIGCLVVQVESVRQALKISTKTDVRIHAEGSVERTVGDIG
                     SPDLRTIGGLLAFDTAS"
     gene            7324..7860
                     /locus_tag="AC16BC_10855"
     CDS             7324..7860
                     /locus_tag="AC16BC_10855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3242159.1"
                     /translation="MFRPHHPICAVVFSVFMSACAASPAPYLAVDQNPAPQTLDYQAL
                     MAAPVKPVTDVRQRVVQTATTHLGAPARQGGDTPEGFDCTGFVFYVYHEAARITLPRK
                     SHDQVQIGKTISPIALQPGDLIYFTVPKSRSFHVGLYVGEGRFIHAPGSNTAVRIDSL
                     GDASWRARFLGARQILPS"
     gene            8018..8731
                     /locus_tag="AC16BC_10860"
     CDS             8018..8731
                     /locus_tag="AC16BC_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR3242160.1"
                     /translation="MRAHVFQHVPFEGPGSIAPWLEARGAKISVTRFFQNDPLPQVGE
                     IDFLIVMGGPMSVNDEHVFPWLVAEKRFIREMINHGKPVLGICLGAQLIANALGSKVY
                     RNSEKEIGWWPIQSTPGAEHPHAVFRFPDHCVVFHWHGETFDLPQGAVRLAKSAACAN
                     QAFQFGANVIGLQFHLETTPASARELVANCRDELVPAKYVQSEDSILGVPPDQYATIN
                     ALMGQVSAFLVPDHESGRR"
     gene            complement(8790..9323)
                     /locus_tag="AC16BC_10865"
     CDS             complement(8790..9323)
                     /locus_tag="AC16BC_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3242161.1"
                     /translation="MAPAFAGIIDAKLPWTYNHSTGVADIAHGIGTLLGLPAPALRDL
                     RRAALLHDVGEPGVSSSILDKPGKRTDDEFVQMRRHSHYTEQILGRVDALHHVSELAC
                     SHHERLDGKGYHRGLTTEELSTTTRILPIADIYQALSIMRKDVGSALCPRAFAGLEQY
                     LDRFSAQDAAARLRKAA"
     gene            9828..10307
                     /locus_tag="AC16BC_10870"
     CDS             9828..10307
                     /locus_tag="AC16BC_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HIR3242162.1"
                     /translation="MTTPIRRIDQALLDRVTREAARHARLRMNYNFHQHEEPVQRFLN
                     AVEPGSYVRPHRHDDPPKWELFVCLRGRGAAMVFDDDGRIAQIHRLDPAQGDYGVEIP
                     AGVWHCIVSLSPGSVFLEIKEGPFEPKHAGVFAPWAPAPDDPGAAAFLAALERAASA"
     gene            complement(10304..10600)
                     /locus_tag="AC16BC_10875"
     CDS             complement(10304..10600)
                     /locus_tag="AC16BC_10875"
                     /inference="COORDINATES: protein motif:HMM:NF025363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HIR3242163.1"
                     /translation="MSARLTEIQSRREALVGRAAEQRDALNELIEPWRARLAVADRLI
                     AFARKLHAGWITLAAGALVISQLGKGARGKWIASIWTFWNVYNALRGRGPRSRS"
     gene            complement(10605..11000)
                     /locus_tag="AC16BC_10880"
     CDS             complement(10605..11000)
                     /locus_tag="AC16BC_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3242164.1"
                     /translation="MTKPDQGEDPRGTGLLDSVQRLAVTLIEIVHTRVDIVATEFEEE
                     RERIRELALFGLMALFFAGFGFLLLTLFLIVLFWDTHRLLAVGGFTVLYLGLGGFAAA
                     TLRRRLKTRPRLFATTLAELSKDREHLSR"
     gene            complement(10993..11313)
                     /locus_tag="AC16BC_10885"
     CDS             complement(10993..11313)
                     /locus_tag="AC16BC_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ElaB/YgaM/YqjD family protein"
                     /protein_id="HIR3242165.1"
                     /translation="METATDRAAVTDKLVQDVKAVIADTEELLKATAGQTGEQIAAVR
                     AKLVARINASRQHLADLEHGAVEKAKAAAEATDKLVREHPWPAMGVAAAVGFLLGLLT
                     SRRD"
BASE COUNT         1978 a         3729 c         3880 g         1776 t
ORIGIN      
        1 ggcgcgttgt agtcgtccgg gtacggcgcg ggcaccggcc gatcgagcac gaagtacaac
       61 gccggacgat tggtcaggtc gagataggtt tcgccgtcgc gcagcaggcg acgcagggcc
      121 gcgttcaggc gctggatcgc gtccagcttg tccggcagca cgaagccctc cccggcgttg
      181 ggcacgcccg ccgcggcgcc ggagcgctgg acggtgcggg ccggcgcgtc gatcgccgcg
      241 atcgcgcgtt gcgcgatctg ccaggggttc ggatcaccga acgcgccgcg cacgccgagc
      301 aacgcgccga tgacccccgc cgcgatcagt gcggcgcgcg ggtcgggacg acgcgtgagg
      361 atgattgcca ggggcagcag gccgccgatg gcgagcatcg acgccgcgcc cgagcggctc
      421 aattcgtccg gccagacctg tcccaggctg taggggatga gcgcgagaag aaagagcacg
      481 ccggtgagcg tgatcacgac cccggcgggt tcgaccgggt cgccgcgccg ctgtctcgac
      541 acctgatgca cggccaacgc gacgagggcg atcgctccgc ccacccatcc gccgatccgg
      601 atcagttccc acgccagctt gttgtcatgc caccaaaacc agtccgggac gcgcccggcc
      661 tgcaacacgc cgacgccgaa cgccgtggtg ttggtcggcg cgttttcaat cacaaaccgc
      721 gcgaggccga gaagatacgc gccgagcgac ggtgccagcg cggcgccgag tcctccgatc
      781 gcgaacatca tggcggtccg gcgaggggtg atcatcgacg gcgcgatctc ggcctgcggt
      841 gttcgcgcgc gcaccgccat ccacgcggcc accggggccg tggccgctgt gcaggccaac
      901 cccacgatcg cgttgtagag gacatggacc atcgacaggc ccgcccacgc catcagccat
      961 cgcctcggcc gggccaggag agtcgggtgc gccagtacag ccagaatcgg gagcgcgagg
     1021 aagaagcggt tgaagctggc caacaccgac gtgtacccgg tcggtagaaa cggcgcgagc
     1081 agcaaggccc atcctgggcc gcccaggcgg cacacggtcc aggccgctcc agcggccgcg
     1141 gccaccgaca accacgacac cgcgatcggg aacgacgaca cggcgccgtc caaggccagc
     1201 gtgttgaccg cgccgtacaa caggctcatg ccgccttgtg ccggcacgaa ctggtcgaac
     1261 agtgcgcggt catgcgtcac gacctggtgc cacggcagca ggagctcccc gaggtggagc
     1321 gagtcccact caaacccgct gaactcgggc acgcggtacg cgagaaacgc ggcgatccag
     1381 accacgctcg acggacggat catccggttc gcgtccggca ccacgagcca tggttcggcg
     1441 cggcgccacg cgatcatctc gcgcgcgcac atggccgcgg cgaacacgag cacgcccgcc
     1501 ttggtcatgg tcggcatatt gagccgcgtg acaaggccgt cgtgcaggta cgcgtcgtgc
     1561 gccaacaccg tgatcagcaa cggcagcggg atctggctgc ccgacgcgat cgcgcgccag
     1621 gtcgcagggg agaaccgccg atgcccaagc acgagtccgc acgcgaaaat gaacggggtg
     1681 gtgagcgcga acgcgctcca gagggacgtg tgtccggcga accatgtcgg gatcacggcg
     1741 tccatggccg cgagcacgcc gaggagcgag aagtaggcgc agacaaccgc gacgaggagc
     1801 gcgacgaatg ccgggccggc gggtacgatc gccgggatca cggttgatcc gcgagtcagt
     1861 ccggcccacg cggccggcag caccagcgcc gcggcgagcg gaaaccacgg ccaacgtcgc
     1921 gtgacgagtc cgaacgcggc ggcgtacgtc gcgatgccga gatacagcgc gtagggaacc
     1981 gcgggcgttt cgatcatcac ggcgccgcgc tcgggccacg gtagttgatt ggccagtcgg
     2041 ctcaggatcg gccacgcgaa cacgaaggcg gcgaagaacc acgcggcggc aacatactcg
     2101 gaggacttgg ggtagtcgct ccaggtgatc atgcccgcga ccaggtcgga atagggctgg
     2161 ggcgcggctg cgagccgagg ccacgcggac acggtcgcga tcgcggcgag cgccgcggcg
     2221 ccgatggtca cggcgagcac gcgcgcgccg gatcgcggca gccaccacgc gtcaacgcgg
     2281 ccgctcacgt cagtccatac gcggtccgcg catgccggag ggcctcgtcc acgcggtcga
     2341 tggaccgctc gcggtccgcg acccgcatct cgatcgagac ccacgaggaa tacccgatgc
     2401 gtcgcagcgc ggcggcaaac gccgcgtgat tcaccccgcc ggaaccgatc ggggccaggt
     2461 tcggttcact cgcgtggacg tgggcgacaa cggttcccgc ctcgcggatc acggacgccg
     2521 cgtctggttc ggcggcggcg ctggccgcat cgagatgcaa tccgaacccc gcgctgtcga
     2581 cgcgacgcac cagatcaagg ccttcacgga tcgtggtcac gaactcgcat ccccacgagc
     2641 gcggaatggg ctcgatgcaa agctgaacgc cgtagtgctc ccccaccctt ccgacgccgc
     2701 gaaagaacgt ctcggcgatc tcgtccgctt cacgcggagc caggccggcg gtgcgtcggt
     2761 gtccggggga gccgaacacc aacacccggg cacccaggtc aaccgccagg tggatcagtc
     2821 ggaagagata gtccatggtc gcggctctcg cgtccggtcc ggaaaaaatc gcgagttccg
     2881 gccgcccgta taacaaggct tggaaggcga cgatggcgat gccgcggcgt tcccacgcgc
     2941 ggcgatactc cagccgttcc cgtttggagg atgcggtcgg gtccgaccag accgcgctcg
     3001 gcgcgatttc caccccgcgg accccgcgcg tttcgaggag cgcggccgcg gcgtgttcct
     3061 gatcgcgcgg ccacgcgatg ttggagacgg ccaggttcac acctgcgccc gcgcctgctt
     3121 cacgaacgcc gtgatgcggc gcacgctctc ggtctttgcg gcaaggtagc cgctccgtcc
     3181 gccgaagagc cggtcgtact cggttcgcac gtcatagtgg gtcgcaggcg ccgatgcccc
     3241 ggcggcggcc agctccagtc cgaacgcgtc gcgcgccagc tcatcgacgc ggagcggttc
     3301 ggtgaccagg ttgaccagcc ggaggcgctt cgccagcgcg agacggatat cgatccagag
     3361 acggtcgatc ggataccact gatacgtgct gtcggggttg atctgttcga tgccgcgccg
     3421 gttcagcaag tcaaagatga cgttctttct caggccgggt ccgaacaggc cgggcagtcg
     3481 gatcacgacg gcgtcgaacc gggcggtcac gacgtcttcc agttgccgcc ggtgccgacc
     3541 gtacggggag gcgtcggttg gaacaaccgt ggtggcttcg ctgactccga ccgcgttcgg
     3601 gtacacgtcc acggtcgaga tcagcaccgc gcgccgcgcg gtcacctgca tgagcgcggc
     3661 ggtgaggcgc gcgatcgccg ccgcgtccgc gaccgggtcc tgattcgctc gccatttggc
     3721 gccgggcgcg cccgcgatga tcagcaggtc gaagcaacga ccgacgatgg cgtcgatcgt
     3781 gccggaattg taggtcgcat ccaccggcac ctgccgcagc aggttgccgc ccacgaagcc
     3841 ggtgtctccg attaggctga cggccatcgc gtctccctct catccggaga gatcgggggg
     3901 gcgtatcgcc cacgccatac ggcccagagc gccgaaagga tttccaggtg cccgaccagc
     3961 ggccggatct tggtcggcac ccgtcccggg ggataccggc gcgtgaccgg gacctcgatc
     4021 acgcggtagc cgaggcggcc ggcttgcgcg gcgaggtaat acgggagttc gtatccccgg
     4081 aacacgggcc ggaacggcgc cacgcgcgga tcgagcaaga accgccggct gtagccgcga
     4141 aacccgttgg tggtgtcggt gtatcgcacg cccgccgcca ggctgatcac gggcgcgtgc
     4201 aagagcctga tcgctacgag gcgcgtccag ggcgtggcga cggcgcgtcc gccggggacg
     4261 aaccgggagc cttgaacatg gtcgtagccg agatcgagcg cgcgcagcac gagcgggatc
     4321 gacgcggggt cgtccttgtt attgccgtcc atggtcgcga cgccgtcgta tcccgcctcg
     4381 agggcgtagg caaaggccag gcgcaactgc gtgctcaatg cgcccggctc gctcttgggg
     4441 atcacggcgc ggaccccgaa ttcgcgcagg gcggggagcg cgaccgaccc gtcggtgctc
     4501 cctccgtccg cgatcacgac gtcgacgctt ccggcgatgt cccgcatgcg atagagttgg
     4561 gcgtgcaggc gctcaccctc gttgatcacc ggaatgcaga ggcagtaccg ggaagcgcgc
     4621 ggcccgagga cggccacgcg cggcgcgctc acgtcagagg cggaaccgga cggcgccgga
     4681 tgcgcgacca tggtcacagg cgcaccgttt gcgggggatc gaccagcacg ttgcgatgcg
     4741 ggaccgcggc gagcagcagc gggctggttc gctcatccct gatgtaatag agcggtcgat
     4801 tccacgactc gtccaacaat cgggcgagat actcgcacac gacgcccagc acggccagca
     4861 cggtggcgaa catcgacgcg gccagcgacg agagcgtggc ccagccttcc gccacgtgac
     4921 gtgtgaagag ccaggtgccg gcaacgtacc cgagcgccag gaaactgacg acgccgccga
     4981 gcagcgcggc gcggccggcc aagcgaaggg gcgccgtggt gttgcccgag atcacttgaa
     5041 aggccaggcc gatcccttgg aaaaaccgcg tgacgttgcg gttgcctgac cggctctgcg
     5101 ggtggtaggt gaggctgtcg gtgccgtatc cgatgtacgc gctggacacc cagagcgacc
     5161 ggcgccggtc cttgatctgg ccgatggcgt ggaccgcctg gcggctcaac gcgcgaaaat
     5221 gggtggcgtt gctggggagc gccagcccga agacccgccg gcacagccaa taaaacaacg
     5281 cggcgcctat gcggaccaac agggattccc ccgcgcgatg actgcggacg ccgaacacca
     5341 ccccggagcc cgccgcgcaa cggcggacca tggtcggaat cagttccgga gggtccgaat
     5401 ccggcgacat caccacgacc acgtcgccga tcgccagatc caagccggcg gtaatcgcga
     5461 tctcggcgcc gaacgcgcgg gacagggtga cgacgcggac gtgatcgtag cgatcaaaga
     5521 cggacaccgc ggcggcgacg gtgccgtcgg tcgacgcgtc gtcgacgagg acaatctcgt
     5581 agtgtcggaa gtggcgcctc agcaccccgt gcgtctcggc cagaaactcg gtgatgatgc
     5641 cggcgttgtt ccgaagcgga gcgaccaccg aaaccacggg ttcgagcccg acgctcacgc
     5701 acggcctccg gaggctgcca tcagatcgag ttcggacagc aggtcgtaga cctggtcgat
     5761 cttggctccc acgatcgagt ggaagttcgg gaggcccggc gtgctcgcga tcacgatggg
     5821 gcgactgtcg tcgctcttgc tggacggcag ggcggtcttg atctcccaga gcgagtccac
     5881 gtggcggcac tccctgagga cgggaacgaa ccgggccgcg tccgcgacca tgtgcgtgta
     5941 gcggctggcg cggcccaggc gcgctgggga cggaggcccg tccggggggg cgtccgggcc
     6001 gtccgtccaa gccacgtgcg gcgtgtaccg cacatggctc aatgagtgga ggccgcgggc
     6061 cggaaacggc atgatcgaga agaacggccc gcacaccacc gtgatcgcgc ggcgccgcag
     6121 cgcgtcgggc ggctccacca gcgcgagttc cgccaattcg tatcgcaggg gcagcgtcgg
     6181 cagtcccgag tttcgcagca gccggttggc gcgcgcgaac gtgcagttca acacgcgttc
     6241 cgcgagcagg gcgccggacc ggccgccgcg ttcaagggtg agttccatcc gcccgtcccg
     6301 cccgcgtcgc acggcggtca cgtcggtgtt gagcgccagc gagacgcccg agcggtcgat
     6361 ctgcgcacgg agggcgcggc ggagtctgac cgcgtcgaac gcatactctt gcaccaggaa
     6421 cacgtcctcg atcaggccgg ggttgaacag gcgccgaact tcggccggtg cgggctcgat
     6481 cggcgcgctg atgcggtcgc aaaacgtgcg aaacctcgag gcgctgattc cggcgagcga
     6541 attgatcgtt gagacggcgt agtagtgctc gaagccgcgg tcgatgcagt ccggatattc
     6601 ctcgacgaac ttggggaagt tgacgcggga gcgcagcgcg gtcaaaaaac ttcggggata
     6661 gtggtacccg tggtgcacgc gcgcctggtt cgcgaacgac gcgcgccgga ggatgtcggc
     6721 ttcgcgctcc acgagcacga cccgctccaa tccttggcgt ttgagctcca aggccaagcg
     6781 gcagccgaag aacccgcccc cgataatcgc ggcgtcccac gcgtcggagg aagagacgtc
     6841 ggcgtcgcgc ctcgcgatgt cctcagcatc ggctccgtgc ggcgccatgg aggcgaacct
     6901 agcacgcggg gcattaagaa tcggttaagg ggtcggaccc gcggttcaga ccgcgtggcg
     6961 ccggcgacgc ctcacgaccg cttcggcggg gtgatcggta cccggcggct gaagaagacc
     7021 gaatagggat acctcggttg acagcgagag cgcgacggat atgctttggt ccgtcggtac
     7081 acgcattgct cggcggcatc atcgggcggg gggtgtgcgt gaaacgtttt gtgatcggat
     7141 gtctggtcgt tcaagtcgaa tccgtgaggc aggccctgaa gatcagcact aagaccgatg
     7201 tccggatcca tgcggaggga tcagtggaac gaaccgtggg ggatatcggc tcaccggacc
     7261 tgcgaaccat cggtgggttg ctggcgttcg acaccgcatc ttaaggtgct cggtactgtc
     7321 gacatgttcc gtcctcatca ccccatctgc gccgtcgtgt tttccgtgtt catgtcggcc
     7381 tgcgccgcgt ctcccgcgcc gtacctggcg gtcgatcaga acccggcccc gcaaaccctc
     7441 gactaccagg cgttgatggc cgcgccggtc aaacccgtga cggacgttcg ccagcgggtg
     7501 gtccagaccg cgaccacgca tctcggggcc ccggcgcgac agggcgggga cacgcccgag
     7561 ggattcgatt gcaccggctt cgtgttctac gtgtaccacg aagcggcccg gatcacgctg
     7621 ccgcgaaaat cccacgacca ggtccagatc gggaaaacca tctcgccgat cgccctgcaa
     7681 cccggcgatc tgatctactt cacggtgccc aagagccggt ccttccatgt cgggttgtac
     7741 gtcggcgaag gacggttcat ccacgcgccg ggcagcaaca ccgcggtgcg tatcgacagc
     7801 ctcggcgacg cgtcctggcg cgcccgattc ctgggcgcgc ggcagattct tccgagttaa
     7861 tccttagatg cgagcgggcg ttgcccgcgg ctccttgacc cgtgatccgc cccgggacta
     7921 tgctgcggtc gcggtccggg cctttgccac caggcttgat gggacacccc cttaaacaga
     7981 tacgcgccgc tcgatccacc aagtcaataa gcttgtgatg cgcgcccacg tattccagca
     8041 cgttccattc gaaggtccgg ggagcatcgc gccctggttg gaggcgcgcg gggccaagat
     8101 cagcgtcacg cgcttctttc agaacgaccc attgccccaa gtcggggaga tcgacttttt
     8161 aatcgtcatg ggcgggccca tgagcgtgaa cgacgaacac gtcttcccgt ggctcgttgc
     8221 cgagaagcgc ttcatccgcg agatgatcaa tcacggcaag cccgtgctgg gcatctgcct
     8281 gggcgcgcaa ctgatcgcga acgcgttggg ttcaaaggtc tatcgcaact cggagaagga
     8341 aatcggctgg tggccgatcc aatcaacgcc cggagccgaa catccgcacg ccgtcttcag
     8401 atttcccgac cactgcgtgg tatttcactg gcacggcgag accttcgacc tcccgcaggg
     8461 cgccgtgaga ttggccaaga gcgcagcctg cgccaaccag gcgttccagt tcggcgcgaa
     8521 cgtgatcggc ctgcagttcc acctggaaac cacgcccgcg tccgcgcgcg aattggtcgc
     8581 gaactgccgc gatgagttgg tgcccgcgaa atatgtccag tccgaagaca gcattctcgg
     8641 agtacctcct gaccagtacg caaccatcaa tgcgctgatg ggccaggtgt cggcgttcct
     8701 agttccggat cacgagtcag gccgtaggta ggccgatccg gtggggaggc ggggtgtggg
     8761 gcgagacgaa ctgcgcgggt accagagcgc tatgccgctt tgcgcaggcg cgcggcggcg
     8821 tcctgcgcgc tgaagcggtc gaggtattgt tccagcccgg cgaacgcgcg ggggcagagc
     8881 gcggagccga cgtccttccg catgatgctg agcgcctggt agatgtcggc gatcggcagg
     8941 atacgcgtcg tggtggagag ttcctccgtg gtcagcccgc ggtggtagcc cttgccgtcc
     9001 aatcgttcgt gatgggaaca ggccaactcg gacacgtgat ggagcgcgtc gacgcggccg
     9061 agaatctgct cggtgtagtg ggagtgccgg cgcatttgca cgaactcatc gtccgtgcgc
     9121 ttgccgggtt tgtccaggat cgagctcgac acgccgggtt cacccacgtc gtgcaacagc
     9181 gcggcgcgtc tgagatcgcg cagggccggg gcgggcaggc ccagcagcgt gccgatgccg
     9241 tgcgcgatgt ccgcgactcc ggtggagtgg ttgtaggtcc acggtaattt ggcgtcgatg
     9301 atgccggcga atgccggcgc cacgcgatcc aggcgcgcgc tgtccagccg gagccgttgt
     9361 gtcgatgatt ccgcgcgtgc gtcgctcgtt caaaaattgt atgctgtccc ggtgttcgct
     9421 gctgatcgaa tggggcgtcg acgcgtcgcg tctgtcgctg gcgtctcact aggtgtttga
     9481 cgtgccacca tcgtttcgtg tcgaccgcac gatgaagaac tcctggttca tcccatggca
     9541 ttgttcgctg gcgcgcgatc tggacggtgt gttggcggcg ggaacgcaga ttgtcccgaa
     9601 tttacgtcgg gtcgaaccgg actttagctg aactatctga aaagacaggc gtttggtggc
     9661 ccataaattg ggcaatgtgc gcaaacgctg ataaggtggg gctcggattt tccaatccac
     9721 atggttgacc acaagtcatc cacaggacat gaggactcgt gcgatcgtcc atgcgcgatt
     9781 aaatcggtga ctcgacacca cgatcccgat cgactctcgg atctcggatg accactccga
     9841 tccgccgcat tgaccaggcc ctgctcgatc gcgtcacccg cgaggcggcg cggcacgcgc
     9901 gtctgcggat gaactacaac ttccaccagc acgaagagcc agtgcaacgc ttcctcaacg
     9961 cggtggagcc ggggagttac gtccggcccc accgtcacga cgatcctccc aaatgggaac
    10021 tgttcgtgtg tctgcgcgga cggggcgcgg ccatggtctt cgatgatgac gggcggatcg
    10081 cacagattca ccgactcgat cccgcgcaag gcgactacgg cgtggagatc ccggccggcg
    10141 tgtggcactg catcgtcagt ctgtcgccgg gttcggtgtt tttggaaatt aaggaggggc
    10201 cgtttgagcc gaagcacgcg ggcgtgtttg cgccgtgggc gccggcgccg gatgatccgg
    10261 gggcggcggc gttcctggcc gcgctggagc gcgcggcgtc cgcttaggat cgactgcggg
    10321 ggccgcgccc acgcagggcg ttgtagacgt tccaaaaggt ccagatgctg gcgatccact
    10381 tgccgcgggc gccttttccc agttggctga tgaccagtgc gcccgcggcg agcgtgatcc
    10441 aacccgcgtg aagcttgcgg gcgaatgcta tcaggcgatc cgctacggcg agacgggcac
    10501 gccacggttc gatgagctcg ttgagagcat cccgctgctc cgctgccctc cccaccagcg
    10561 cttcccgtcg ggactgaatc tcggtcaacc gcgcgctcat gccttcaccg actcaggtgc
    10621 tcccggtcct tggagagttc ggccagagtc gtggcgaaca accgcggcct ggtcttgagc
    10681 cgccgcctca aggtcgcggc cgcaaagcca cccaacccca gatagagcac cgtgaatccg
    10741 cccacggcca acagacggtg cgtatcccag aacaggacga tcagaaacag ggtgagcagc
    10801 aggaaaccga acccggcgaa aaacaacgcc atgagcccga acagggccag ttcgcgaatc
    10861 cgctcgcgct cttcttcaaa ctcggtggcg acgatgtcga cccgggtgtg cacgatctcg
    10921 atcaacgtga cggccaaccg ttgcaccgag tcgagcaagc ccgtgccccg gggatcttcg
    10981 ccctggtcgg gcttagtcac ggcgggaggt caggagtccc agcagaaacc cgacggccgc
    11041 ggccacgccc atcgcgggcc acgggtgctc gcggaccagt ttgtcggtcg cctcggccgc
    11101 ggctttcgcc ttctcgaccg ccccgtgctc cagatccgcg aggtgttgtc ggctggcgtt
    11161 gatgcgcgcg acgagttttg cgcgaaccgc ggcgatctgt tccccggtct gccccgccgt
    11221 ggctttgagc aactcctccg tgtccgcgat cacggctttg acgtcctgga ccagtttgtc
    11281 cgtgaccgct gctctgtcgg ttgccgtctc catcgcattc ctccttggtg tcattgcgac
    11341 gcccgcattg tactacgcgg cgc
//
LOCUS       DBNGTT010000476         3962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_881338, whole genome shotgun sequence.
ACCESSION   DBNGTT010000476 DBNGTT010000000
VERSION     DBNGTT010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_881338"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="AC16BC_10890"
     CDS             <1..438
                     /locus_tag="AC16BC_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3242166.1"
                     /translation="PTTALDVTVQAQILTLLRDLRERLGMAMVFISHNLGVIAQVAHR
                     VVVMYAGRIVEQTTAAQLFAQPQHPYTKGLLASLPPADFHIGRAGRLSAIPGVVPVLG
                     SIPGGCTFAPRCPVVVERCVQEPPLLSTGNDHLTRCWLVEPPP"
     gene            441..1451
                     /locus_tag="AC16BC_10895"
     CDS             441..1451
                     /locus_tag="AC16BC_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3242167.1"
                     /translation="MTPVSPQPAPASRLDPDVLLDVTGLAKYFPVRSGAFGMHRTQRV
                     HAVDGVSLRINRGEVLGLVGESGCGKSTTARLILRLIEPSAGTIRFLGEDLLSAGAAR
                     MKALRKHLQIVFQDPFSSLNPRMRIGSILEEPLSIHGLGSRAERADAVRGIIEKVGLT
                     PDALARYPHEFSGGQRQRIGIARALILKPQLVVADEPVSSLDVSIQAQIINLLQDLRR
                     EYQLSLLFIAHDLVMVRHISDRVAVMYLGRIVEFASSDALFTHPRHPYTQALIAAIPV
                     PDPAVAPRTMLLEGDPPSPINLPTGCRFYSRCPKRVPECQTIDPPLRQIENGHEVACI
                     LA"
     gene            complement(1468..2481)
                     /gene="trpS"
                     /locus_tag="AC16BC_10900"
     CDS             complement(1468..2481)
                     /gene="trpS"
                     /locus_tag="AC16BC_10900"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3242168.1"
                     /translation="MAGRPRILSGMQPSGRLHLGNYVGALANWVTLQDTHECFFMVAD
                     WHAVSTNYAETSRIRSFTREMLIDWLAAGLDPNRSTVFVQSSVPEHAVLHLLLSMIVP
                     VPWLERNPTYKEQQEQLADRDLTSYGFMGYPVLQSADILLYRADAVPVGQDQLPHLEI
                     TREIARRFNHIYGVVFPEPKALLTQAAKLPGTDGRKMSKSYNNAIYLSDTEPTVRGKL
                     RTMVTDPARKRRSDPGNPDVCPVFDLHKIFSPAAVRERVNAECRTAAIGCIDCKSLLA
                     DGVVARMQPIWERRTALEQEPSQIDRAIKSGAEKARAVAAETLNAVNTAMHLGVDAPP
                     SPS"
     gene            complement(2584..3234)
                     /locus_tag="AC16BC_10905"
     CDS             complement(2584..3234)
                     /locus_tag="AC16BC_10905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3242169.1"
                     /translation="MSIAALPVLLAITLHEAAHGYVADRKGDPTAKYLGRLTLNPLAH
                     IDPFGTILLPLMLLYFGGIMFGYAKPVPVNFMNLRRPKKDMVWVAAAGPLANLGLAVL
                     FGLVLRLIAGFSGVSTTTGEPGNESGFQIMEPIVQMLLFGVVINVLLMLFNLLPIPPL
                     DGGRVLVGLLPDAVARRVAGLEPYGMLIVILLVLEPLHLIFRILDPLRTALINLIL"
     gene            complement(3265..>3962)
                     /locus_tag="AC16BC_10910"
     CDS             complement(3265..>3962)
                     /locus_tag="AC16BC_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3242170.1"
                     /translation="DRYSQEVATAFGSEVGRLGGRLVYSGSYQSGSVDFGAEIKSLKE
                     ADLKQEGVLEQLPVEAGTPAAVPGAPPKEPVYLPGFDALFLPGDAETSGLLASQVRFF
                     DIAVPLLGDSGWNDRTVIKAGGKFVEDAIFADVFFADAQDPAVQRFVRDYRARYHAAP
                     DVFAALAYDATAALVRGIKSGATTGERLREELTKMNGYAGVTGLTGFGVDGEAQRRLS
                     WIQIRNGRFVPAL"
BASE COUNT          736 a         1323 c         1190 g          713 t
ORIGIN      
        1 ccgaccaccg cgctcgacgt cacggtccag gcccagattc tgacgctgtt gcgggacctt
       61 cgtgagcggc tcggcatggc gatggtcttc atctcgcaca atctcggcgt cattgcgcaa
      121 gtggcgcacc gcgtcgtggt tatgtacgcg ggacggatcg tggaacagac caccgcggcc
      181 caactctttg cacagccgca gcatccttac accaagggac tactcgcgtc gctccccccg
      241 gccgacttcc acatcggtcg ggccggtcgc ttgtcggcca ttcccggcgt ggtgcccgtg
      301 ctgggctcga ttcccggcgg ttgcacattt gcgccgcgct gtccggtggt agtggaacgg
      361 tgtgttcaag aaccgccact tctatccacg ggcaacgatc acctcacgcg gtgttggctc
      421 gtggaaccgc cgccatgacc atgacgcccg tttcgcccca accagcccca gcgtcacgtc
      481 tcgaccccga cgtgctgttg gacgtcaccg gcctcgccaa atatttcccg gtccgatccg
      541 gggcgttcgg catgcaccgc acgcagcgcg tgcacgccgt cgacggggtg tcgttgcgga
      601 tcaaccgcgg agaagtgctc gggctcgtcg gggagtcggg gtgcggtaaa tccacgaccg
      661 cccgcctgat cctgcggctc atcgaaccgt cggccggaac catccgcttt ctgggcgaag
      721 acctcctcag cgccggtgcc gcccgaatga aggcgttacg caaacacctc caaatcgtgt
      781 ttcaggatcc gttctcttcg ctcaacccga gaatgcggat cggctcgatc ctggaggaac
      841 cgctcagcat ccacggcctg ggctcacggg ccgagcgagc cgacgcggtt cgaggcatca
      901 tcgaaaaggt cgggctcacc cccgacgcgc tcgcccggta tccgcacgaa ttcagcggcg
      961 gccaacgcca gcgcatcggc atcgcccgcg ccttgattct caaaccgcag ctcgtggtcg
     1021 ccgacgaacc cgtctcgtcc ctcgacgtgt cgattcaggc ccagatcatc aacctgttgc
     1081 aggacctgcg gcgcgagtac caactctccc tgctgttcat cgcgcacgac ctggtgatgg
     1141 tccgccacat cagcgaccgc gtggccgtga tgtatctcgg tcgcatcgtc gaattcgcgt
     1201 cgtcggacgc gctgtttaca cacccgcgcc atccctacac gcaggcgctc atcgccgcga
     1261 ttccggtgcc ggatcccgcg gtcgcccccc gcaccatgct cctggagggt gatccgccga
     1321 gccccatcaa cctgccgacc ggatgccggt tctattcgcg ttgtccgaaa cgagtccccg
     1381 agtgccagac cattgatccg ccgctgaggc agatcgagaa cgggcacgag gtggcgtgca
     1441 tcctcgcgta atgtagggac cggggaacta cgacggagat ggaggtgcgt cgacgccgag
     1501 gtgcatcgcg gtgttcaccg cgttcagcgt ctcggccgcc accgcgcgcg ccttctcggc
     1561 gcctgatttg atcgcgcggt cgatctgaga cggctcctgc tccagcgccg tcctgcgctc
     1621 ccaaatcggc tgcatgcgag cgaccacgcc gtcggccagc agtgacttgc aatcgataca
     1681 cccgatggcc gcggtgcggc actcggcgtt gacgcgttcg cgtaccgcgg cgggggagaa
     1741 aattttatgg agatcgaaga ccgggcacac gtccgggttg ccgggatcgc tgcgccgctt
     1801 gcgcgcggga tcggtcacca tagtccgtag tttcccgcgg acggtcggct ccgtatccga
     1861 caggtagatc gcgttgttgt agcttttgct catcttgcgg ccgtcggttc ccggcagctt
     1921 ggcggcctga gtcagcagcg ccttgggctc ggggaaaaca acaccgtaga tatgattgaa
     1981 tcggcgcgcg atctcccggg tgatctccag gtgcggcagt tggtcctgcc ccaccggcac
     2041 cgcgtcggcc cgatacagca gaatatccgc ggattgcagc acgggatacc ccatgaaccc
     2101 gtaactcgtg agatcgcggt cagcgagttg ctcctgctgt tccttgtacg tcggattgcg
     2161 ctcgagccac ggcaccggaa cgatcatgga cagaagcagg tggagcaccg cgtgctccgg
     2221 gaccgaggat tggacgaaga cggttgatcg gttggggtcc aagcccgccg ccagccagtc
     2281 aatgagcatc tcgcgggtga acgaccggat ccgactggtc tcggcgtagt tcgtggacac
     2341 cgcatgccag tcggccacca tgaagaaaca ttcgtgggtg tcctgaagcg tgacccaatt
     2401 ggccaacgcc cccacgtagt ttccgaggtg cagccgtcca ctgggctgca tcccgctcag
     2461 aattcgcggc cgaccggcca tatccactcc ttattcacga agtcgccgcc cgacaacgct
     2521 ggatcatcac acgacccaat gctgcggggg ctccagtttc tgtcgttgcg cggccatggg
     2581 aatctagaga atgagattga tcaacgccgt gcgtagtggg tccaagatac gaaagatcaa
     2641 atgcagtggt tccaagacga gcaatattac gatcagcatt ccgtagggtt caagccccgc
     2701 cacccgccga gcgaccgcgt ccggtaacaa cccgacgagc acgcgcccac cgtcgagtgg
     2761 gggaatcggg aggagattga aaagcatcag gagcacatta atcaccaccc caaacagcag
     2821 catctggacg atcggctcca tgatctggaa ccctgattcg tttcctggct ctccagtggt
     2881 ggtcgaaaca ccagagaatc ccgcgatgag gcgaagcact agaccaaaca gcaccgcgag
     2941 gccgaggttc gcaagcggcc cggccgccgc cacccacacc atgtccttct tgggacgacg
     3001 taagttcatg aagttcaccg ggacaggctt tgcgtacccg aacataatcc cgccaaagta
     3061 caagagcatc aacggcaaga gtatggtccc aaacgggtca atgtgggcca aggggttgag
     3121 ggttaaccgc ccgagatact tggcggtcgg atctcctttg cggtccgcca cataaccgtg
     3181 agccgcttcg tgcagcgtaa tcgccagcag gaccggcagg gccgcgatcg acaccgtgtg
     3241 gagaaattgg gtcaattgca cggtttacaa ggccggaacg aaccgaccgt tccggatctg
     3301 gatccacgac agtcgccgct gcgcctcccc gtccactccg aatcccgtca gcccggtgac
     3361 gcccgcgtag ccgttcattt tggtcaattc ctcgcggagc cgctcgccgg tcgtcgcgcc
     3421 gctcttgatt ccccggacga gggccgcggt cgcgtcgtac gcgagcgcgg caaagacgtc
     3481 cggagccgcg tgataccggg cgcggtagtc cctgacgaac cgctgaaccg ccggatcctg
     3541 ggcgtcggcg aaaaacacgt cggcgaagat cgcgtcctcc acgaacttcc cgccggcctt
     3601 gatcaccgtg cgatcgttcc atccgctgtc gcccaggagc ggaaccgcga tgtcgaagaa
     3661 gcggacctgc gacgccaaca aacccgacgt ctccgcgtcg ccgggaagga acaacgcgtc
     3721 gaatcccggg aggtagacgg gctccttggg cggagcgcct ggcacagcgg ctggagtccc
     3781 cgcttccacc ggcagttgtt ccagaacccc ttcctgcttg agatccgctt cctttaggct
     3841 cttgatctct gccccgaaat ccacggagcc agactgataa gatccggaat acaccagccg
     3901 tccgccgaga cgaccgacct cgctgccgaa ggccgtcgcc acctcctgtg agtaccggtc
     3961 tt
//
LOCUS       DBNGTT010000477         2964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_114389, whole genome shotgun sequence.
ACCESSION   DBNGTT010000477 DBNGTT010000000
VERSION     DBNGTT010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_114389"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="AC16BC_10915"
     CDS             <1..1279
                     /locus_tag="AC16BC_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR3242171.1"
                     /translation="RTRVDLRFHITEGPAVTIGSISLTGNERTSPRVILRELDVRAGD
                     VYNPRRIFDNQRRVAQLGFLREVRLEPEDPETVEPVKALRLAVKERDAGSVDIGLGYA
                     NYEGPRGFAEITYRNLWGSGRRAGLRVEGSRIERKAVLSYRHPWLFDRRLDGKAALFH
                     EVREEETRGYQLSAYGTSLGIERTLIPHLFGAMTYDYQLSHIEAFTEKNPSLLPFDEK
                     RRNIASVTPSLVWDTRDSPFNPSSGLLATIALENASLLLGSQEQFWKVTAGASKFTPL
                     RKSVVLALGIRGGIAGRFGEPGPDILLPPTERFYVGGRSSIRGYDEDTVGPQEAGTPT
                     GGNILLVLNAEVRVALPGSLGLVAFWDGGNVWQDQRDIDSVDVFTYIKKTVGGGLRYN
                     TPVGPLRLDYGHKLNWLPGESHGTLHFTLGHAF"
     gene            1455..2003
                     /locus_tag="AC16BC_10920"
     CDS             1455..2003
                     /locus_tag="AC16BC_10920"
                     /inference="COORDINATES: protein motif:HMM:NF025692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HIR3242172.1"
                     /translation="MALLLFAAEPASGGGESIDRLSVAQQDGRILVSAALNPGLPSQT
                     DEDLRKGLPKDLYYYLVLKQRRRNWFDEEILAVTVKYSLKYDLLKQEYVVSTRLPSGT
                     AQTVVRDFASARELVSRIDRVPVADVRHLNRRRSYVVSVKAEMKAPQLPLYLDYFLFF
                     IPFLEVDTEWRNTAPFRAPDGP"
     gene            2058..>2964
                     /locus_tag="AC16BC_10925"
     CDS             2058..>2964
                     /locus_tag="AC16BC_10925"
                     /inference="COORDINATES: protein motif:HMM:NF039586.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242173.1"
                     /translation="MAAKTPSRRWSKKALRALWLTLILILLSISATTLYYKGLGAPSA
                     LSNNILVLTLVNVNFILVILLTLLLSRNLIKLFFERRHQRLGTGFRTKLTFAFVGLSL
                     IPSLLLFVVASGLLTAAVENWFGLQVERSLTSALGVAQSVYQEHEAGIYTQARSIGRE
                     IVRRDWWQPEAHGALTAFLEAEAAGAGRRSIWLLPPGSDPRRTARWSGAPVRAVSPSA
                     RPPFDDPPDELRAVLSDRSLVRSTDYGDVVWGAVPVVDREGTPLGTVAIESLIPPDLV
                     AKLEGISKTYEDYNQLKTFKNPIKGG"
BASE COUNT          518 a          982 c          948 g          516 t
ORIGIN      
        1 ccggacgcgc gtcgacctgc gcttccacat caccgaaggc cccgcggtga ccatcgggag
       61 catctccctc accggcaacg aacgaaccag cccgcgcgtc attctgcgcg aactcgacgt
      121 gcgcgcggga gacgtctaca acccccgccg gatcttcgac aaccagcgcc gggtggcgca
      181 gctcgggttt ttgcgggaag tgcgcctgga gccggaggac cccgaaaccg tggaacccgt
      241 caaggccctg cgcctggccg tgaaggagcg cgatgcgggc agcgtggaca tcgggctggg
      301 gtacgcgaac tacgagggcc cgcgcgggtt cgctgaaatc acctatcgca atctctgggg
      361 gtcaggccgg cgggcgggcc tgcgggtcga gggcagccgc atcgaacgga aagccgtgtt
      421 gagttatcgg catccgtggc tgttcgaccg gcgcctcgac gggaaggccg cgctatttca
      481 cgaggtccgc gaggaggaaa cccgggggta ccaactgagc gcgtacggca cgagtctcgg
      541 aatcgagcgg accctcatcc cccacctgtt cggcgcgatg acgtacgact accagttgag
      601 ccacatcgaa gcgttcacgg aaaagaaccc gagcctcctg ccgttcgatg aaaagcgcag
      661 aaacatcgcc agcgtcacgc cgtcgctcgt ctgggacacc cgcgattctc cctttaatcc
      721 cagttcgggt ttgctggcga ccattgccct tgagaacgcc tcgctgctgt taggttcgca
      781 agagcagttc tggaaggtca cggccggcgc gagtaagttc accccgctca ggaaatccgt
      841 ggtcctcgcg ctgggaattc gcggcggcat tgccggtcgc tttggcgagc ccgggcccga
      901 cattctgctc ccgccgaccg agcgcttcta cgtcggcgga cgaagttcca tccgcggata
      961 cgacgaagac acggtgggac cgcaagaggc cggcactccg accggaggaa acattctgct
     1021 cgtactgaat gcagaggtcc gggtggcact ccccggctcc ctggggcttg tcgcgttctg
     1081 ggacggtggc aatgtctggc aggatcagcg cgacatcgac tcggtcgacg tgttcaccta
     1141 tatcaagaag accgtgggcg gcggactgcg gtacaacacg ccggtggggc cgcttcgcct
     1201 cgattacggc cataagttga attggcttcc cggggaatcc cacggcacgc tccacttcac
     1261 cttgggccac gcgttttagg cgcctcatcc accgcgggga gacgcggcca catacgcccg
     1321 cggggtgcat tcgtgttaag atgcggcgga tcatgatgca ggccaggacg cggcgcaccg
     1381 gagagcacca gggaaagact gggtctgcga ggaccgcgcc gagaccgcgc gcgcgagggt
     1441 tcggcgtcct gtcgttggca ctgctgctgt tcgcggccga gccggcgtcc ggcggcgggg
     1501 aaagcatcga ccgcctgtcc gttgcgcagc aggacggccg cattcttgtc tcggccgcgc
     1561 tcaatccggg cttgccgtcc caaaccgacg aggacctccg caagggcctg ccgaaggatc
     1621 tgtactacta tctcgtgctc aaacagcggc ggcgcaactg gttcgacgaa gaaatcctcg
     1681 ccgtcacggt caagtacagc ctgaaatacg atctcctgaa gcaagaatac gtggtaagca
     1741 cccgcttgcc gtcaggcacc gcccagaccg tggtgcggga ctttgcctcc gcccgggaac
     1801 tggtgtcgcg gatcgaccgc gtcccggtgg ccgatgtccg gcatctgaac cgccgcagga
     1861 gttacgtggt gagcgtcaag gcggagatga aggccccgca gctccccttg tatctggatt
     1921 acttcttgtt tttcattccg ttcctggaag tcgacaccga gtggcgcaat accgcgccgt
     1981 ttcgagcgcc cgatgggccg taaccgcccg atggccgata gaggtccggg aggccgcgcg
     2041 tcggctggca tcgcgtgatg gccgccaaaa ctccttcgcg acgctggtcg aaaaaggcgc
     2101 tccgcgcctt gtggttgacc ctcatcttga tcctattgtc catttccgcg accaccctct
     2161 attacaaggg cctgggtgcg ccgtccgcgc tgtcgaacaa catcctcgtc ctcacgctgg
     2221 tcaacgtcaa tttcatcctg gtcatcctgc tcacgctcct gctgtcccgc aatctcatca
     2281 aactgttctt cgaacgccgg catcagcgac tgggcaccgg gtttcggacc aaattgacct
     2341 tcgccttcgt cggcctctcg ttgattccct ccctcctgct gttcgtggtc gcgagcggcc
     2401 tgctgaccgc ggcggtcgag aattggttcg gcctccaagt ggagcgctcg ttgaccagcg
     2461 ccctgggcgt ggcccagagc gtgtaccagg aacacgaagc cggcatctat acccaggcgc
     2521 gatccatcgg gcgggaaatc gtccgccgcg actggtggca acccgaggct cacggcgccc
     2581 tgaccgcgtt tttggaggcc gaagccgcgg gcgcgggccg acggtccatc tggttgctcc
     2641 cccccgggtc ggacccgcgg cggaccgcgc gctggagcgg ggcgccggtg cgcgcggtgt
     2701 ctcccagcgc ccggcccccg ttcgacgacc cgccggacga gctgcgcgcg gtcctcagcg
     2761 accgcagcct ggtgcgatcc acggactacg gcgacgtggt gtggggcgcg gtgcccgtcg
     2821 tcgaccggga agggacgccc ctcggcaccg tggcgatcga aagcctcatt ccgccggacc
     2881 tcgtcgccaa actcgaaggc atcagcaaga cctacgaaga ctacaatcag ctcaagacgt
     2941 tcaagaaccc gatcaagggc ggct
//
LOCUS       DBNGTT010000478         4077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_497418, whole genome shotgun sequence.
ACCESSION   DBNGTT010000478 DBNGTT010000000
VERSION     DBNGTT010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_497418"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..382
                     /locus_tag="AC16BC_10930"
     CDS             182..382
                     /locus_tag="AC16BC_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HIR3242174.1"
                     /translation="MGRTNIDLDDRLVAEGLKLTRTKTKRELVELALRELVTKKRRKR
                     LLALAGKVGWRGDLSKTRKSRV"
     gene            379..774
                     /gene="vapC"
                     /locus_tag="AC16BC_10935"
     CDS             379..774
                     /gene="vapC"
                     /locus_tag="AC16BC_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3242175.1"
                     /translation="MILVDTTVWVDFFRGTNSPVNRIFRQLIEQEDDLCLTPLNVTEI
                     LQGIRHEALYEETKDHLLQFPLFHSDGISTFLHAADIYRACRGRGRTIRSTIDCMIAA
                     IAIEHDLTVLHNDRDFVHIAACTRLRLFR"
     gene            831..2273
                     /locus_tag="AC16BC_10940"
     CDS             831..2273
                     /locus_tag="AC16BC_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HIR3242176.1"
                     /translation="MVSGNLTAGQSPIVFGTSGWRAVIADQFTFDHVELVARAIAEYL
                     DAQAGGDGPVIVGYDTRFLADRFAGRAADALAATGRRVLLADRDVPTPVVAHAILRQR
                     AAGGVNLTASHNPPEYCGVKFSPSWGGPAQPADTRWIEARIAQLRQGGTGSFKAARAG
                     SVERFDPREPYLAHVRTLIDFKAVRAARLRVAADVLYGTGRGYLDALLADAGCEVTVR
                     HDWLDPLFGGGAPDPSERRLAELASAVREGGYHLGLATDGDADRFGVVDADGAYVTAN
                     EVLALVLDYLIRTRDVKGGVARSVATSHLADAVAARYGRTVYETGVGFKYIGRLIADG
                     KLVAGGEEAQGLTIGGHVPDKDGILTCLLLAEMVARTGKTLRRQLEELIAQVGPRHYR
                     RSDCAAPAGGAGPLNARLGRLTRIGDRPIVRTAEVEGVRKWYFDEATWMIIRPSGTEP
                     VVRLYVESPSLEQAESLLKAGQAVIAECAA"
     gene            2288..>4077
                     /locus_tag="AC16BC_10945"
     CDS             2288..>4077
                     /locus_tag="AC16BC_10945"
                     /inference="COORDINATES: protein motif:HMM:NF046672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HIR3242177.1"
                     /translation="MAWAALAALIALAWAGAASAQSAVPSASLLRQAQDACRELQEVD
                     PEKAKNLCQALSQQPAQSETPLAPSAMPKEPLLRKPEPAPPVPVSSPAIGRDLAPAEQ
                     PRSELERYFANGGGDGPGLPEPEQALAQFGYELFDQRDATPAVDMPVSADYLIGPGDE
                     FTVTLWGLVDASYTVRVDRNGEITLPRAGVIPVVGVRYEELRPLLERRLAKYYTDFHL
                     SVGMGRLRSIRVYVVGEVQQPGSHSLSALSTAYNALFSAGGPTRRGSLRAIQLIRGGK
                     TVATIDLYDFLLKGDRTRDVRLQHEDTVMVPLIGPTVGVSGNVARPAIYEFKGAGGSL
                     ADALALAGGPKPTADLNRVQIERVQAHERRVAVDLNLSNQEDAARIPLENMDVVKLFP
                     IFESRKGIVRLSGHVVRPGTYELKPGMRIGDLIQSYEQLLPEPSLAYSEIVRYEWPTM
                     ARTVVAFSIRKVMDHDPDNNLVLQDRDEIRVYPASRFQDERFVTIKGEVRHPGRYRLY
                     ERMSLTELIRQAGDPTPEALVERAEIRRKVPNDDAGYERQIMYFRLDQLLDGDDPERR
                     TLEPDDEVVIFGKADAKALADITVRGAVKRP"
BASE COUNT          660 a         1289 c         1461 g          667 t
ORIGIN      
        1 tgtggcgacg ggccctcgtg gtgatccggt gcgggcggtg ctcgtgtatc tcgcgcggat
       61 cgaggaacgc ggactctgaa accgatacgc tcccggttcc tctgtggttg tcagcgtgcg
      121 gtgtcgttga tctatacacc tcaacttgtt attgtgtgtg tatccatccg gggagtctcc
      181 aatggggcgc acgaacattg atttggatga tcggttggtg gcggagggac tgaaactgac
      241 gcggaccaag acgaagcggg aactggtcga gttggcgctg cgggagttgg tgacgaaaaa
      301 gcgtcgcaag cgcctcttgg cattggcggg caaagtgggg tggcggggcg acctgtcgaa
      361 gacgcggaag agtcgcgtgt gatactcgtc gacaccacgg tgtgggtgga ttttttccga
      421 ggcacgaact ccccggtcaa tcgaatcttt cgtcaactga tcgaacagga agacgatctc
      481 tgcttaacgc cgctcaatgt gaccgaaatc ctgcagggca ttcgacacga agcgctgtac
      541 gaagagacca aggaccacct cttgcaattt ccgctctttc actccgacgg catctcgacg
      601 tttctgcacg cggcggacat ttatcgcgcg tgccgcgggc ggggacggac cattcgcagc
      661 acgatcgact gtatgatcgc ggcgatcgcc atcgagcatg acttgacggt gctccacaac
      721 gaccgggatt tcgtccatat cgccgcgtgc accagactcc ggttgttccg gtagccgtcg
      781 gccgcttgcg gccttgcatc cgcggacgcg agtctgctat aagcaccggc atggtctccg
      841 ggaacctgac ggcaggccag tctcccatcg ttttcggcac gtcgggttgg cgggcggtca
      901 tcgccgacca attcacgttt gaccacgtcg aactggtcgc gcgggcgatc gccgagtact
      961 tggacgcgca agcggggggc gacgggcccg tcattgtcgg ttacgacacg cggtttctcg
     1021 cggatcggtt cgcgggccgc gccgcggacg cgctcgccgc cacgggtcgt cgggtgctgc
     1081 tggccgaccg cgacgtgccc actcccgtgg ttgcgcacgc catcctgcgc caacgcgccg
     1141 cgggcggggt caatctcacc gccagccaca atcccccgga gtattgcggc gtcaaatttt
     1201 cgccatcgtg gggcggaccg gcccaaccgg ccgacacgcg ctggatcgag gcgcggatcg
     1261 cccaactccg tcaaggcggc accgggtcgt tcaaagccgc gcgcgcggga tccgtggagc
     1321 ggttcgatcc ccgcgagccg tatctggcgc acgtgcgcac cttgatcgat ttcaaggccg
     1381 ttcgcgcggc caggttgcgg gtcgcggcgg acgtgttgta cgggaccggg cgcgggtacc
     1441 tcgatgccct gctcgccgac gcgggctgcg aggtcaccgt gcggcacgac tggctcgatc
     1501 ccttgttcgg gggcggggcg cctgatccgt ccgagcgccg attggctgaa ttggcgtccg
     1561 cggtgcgcga gggcgggtac cacctcggcc tggccacgga cggggacgcg gaccggttcg
     1621 gggtggtgga cgcggacggc gcctacgtca cggccaatga agtcctggcc ctggtgctgg
     1681 actatctgat ccggacccgc gacgtgaaag gcggggtggc gcggtccgtg gcgaccagcc
     1741 acctggccga cgcggtggcg gcgcgctacg gccggaccgt gtacgagacc ggcgtgggat
     1801 tcaagtacat cggccgcctg atcgcggacg gcaaactggt ggcgggcggg gaggaagccc
     1861 aggggttgac catcggcggg cacgtgccgg acaaggacgg gatcttgacg tgcctgctgc
     1921 tggcggaaat ggtcgcgcgg accggcaaga cattgcgccg gcagctcgag gagttgatcg
     1981 cgcaggtcgg gccccgtcac tatcgccgga gcgattgtgc cgcgccggcc ggcggcgcgg
     2041 ggccgctcaa cgcgaggttg ggaaggctca ccaggatcgg cgatcggccg atcgtgcgca
     2101 cggccgaggt ggaaggcgtg cgcaaatggt acttcgacga ggccacatgg atgatcatcc
     2161 gaccgtctgg aaccgaaccc gtggttcggc tgtacgtgga gtcgccgtcg ctggaacagg
     2221 cggagagttt gctcaaggcc gggcaagccg tgatcgcgga atgcgcggcg tgaccaggga
     2281 tcgcgggatg gcgtgggcgg ctctggccgc gctgatcgcg ctcgcgtggg cgggcgcggc
     2341 atcggcgcaa tccgcggtgc cgtccgcgtc gttgctgcgg caggcgcagg acgcgtgccg
     2401 cgagctgcag gaggtggatc ccgagaaggc caagaatctc tgtcaggcct tgtcgcaaca
     2461 gcccgcgcag tccgagaccc cgctggctcc gagcgccatg cccaaggaac cgttgctccg
     2521 gaagccggag cccgcgcccc cggtgccggt gtcctcgccc gcgatcggcc gcgacctggc
     2581 gcccgcggaa caaccccgct ccgagctgga gcggtatttc gccaacggcg ggggcgacgg
     2641 gcccgggctg cccgagccgg agcaggcctt ggcccagttc ggctacgaat tgttcgacca
     2701 gcgcgacgcc acgccggccg tggacatgcc ggtgagcgcc gactacctga tcgggcccgg
     2761 cgacgaattc acggtcacgt tgtgggggct ggtggacgcc tcgtacacgg tgcgggtgga
     2821 ccgcaacggg gaaatcaccc tgccgcgcgc cggggtcatt cccgtggtcg gggtgcgtta
     2881 cgaggaactg cgtcccttgt tggagcgacg gctggccaag tactacacgg attttcacct
     2941 gagcgtgggc atggggcggc tgcgcagcat ccgcgtctac gtggtgggcg aggtgcagca
     3001 gccgggcagc cactcgttga gcgcgctctc caccgcgtac aacgccttgt tctccgcggg
     3061 cggtccgacc cggcgcggca gcctgcgcgc gatccagttg atccgcggcg gcaagaccgt
     3121 ggcgaccatc gacctgtacg attttctgct caagggggac cgcacccgcg acgtgcgcct
     3181 gcagcacgag gacaccgtga tggtgccgct gatcggcccc accgtgggcg tgtcgggcaa
     3241 cgtggcgcgg ccggccatct acgaattcaa gggcgcgggc ggctcgctgg ccgacgccct
     3301 ggccctggcg ggcggcccca aacccaccgc ggatttgaac cgcgtccaga tcgaacgcgt
     3361 gcaggcgcac gaacggcggg tcgcggtgga cctgaacctg tccaaccagg aagacgcggc
     3421 gcgcattccg ctcgagaaca tggacgtggt caagttgttc ccgatcttcg agagccgcaa
     3481 gggcatcgtc cggctcagcg ggcacgtggt gcggccgggc acgtacgaac tcaagcccgg
     3541 gatgcggatc ggcgacctga tccagtccta cgagcagttg ttgcccgagc cctcgctcgc
     3601 gtacagcgag atcgtccggt acgagtggcc gaccatggcg cgcaccgtgg tggcgttttc
     3661 gatccgcaag gtgatggacc acgacccgga caacaacctg gtgctgcagg accgggacga
     3721 gatccgggtg tatcccgcgt cgcgcttcca ggacgaacgc ttcgtcacca tcaaggggga
     3781 ggtgcggcac ccgggccgct accggttgta cgaacgcatg agcctgaccg agttgatccg
     3841 ccaggccggt gatccgaccc ctgaagccct ggtcgagcgg gccgagatcc gccgcaaggt
     3901 gccgaacgac gacgcggggt acgagcggca gatcatgtat ttccgcctgg accagttgct
     3961 cgacggggac gaccccgagc gccggaccct ggagcccgac gacgaagtgg tcatcttcgg
     4021 caaggccgac gccaaggcgc tcgcggacat cacggtgcgg ggcgcggtga aacggcc
//
LOCUS       DBNGTT010000479         2598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_369048, whole genome shotgun sequence.
ACCESSION   DBNGTT010000479 DBNGTT010000000
VERSION     DBNGTT010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_369048"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="AC16BC_10950"
     CDS             <1..664
                     /locus_tag="AC16BC_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010476458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3242178.1"
                     /translation="APMPGMDHGGQGDMGAMPGMDHGAMENMGTDTMPGMAPGEAPMV
                     MHGPDTHGKGNSMVAMMPRSRLHEPGVGLEDTGTRVLAYTDLRAREPYDHRSPEREIE
                     LHLTGNMDRYIWGFNGRKYSESEPIPMRYGERLRVVLVNDTMMEHPLHLHGMWMELDN
                     GNGDRNPRKFTINVKPGERLSFLMTADALGAWPFHCHLGYHMAAGMMAHVVVSEHGTE
                     TH"
     gene            664..1416
                     /locus_tag="AC16BC_10955"
     CDS             664..1416
                     /locus_tag="AC16BC_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HIR3242179.1"
                     /translation="MTNANAVITGIVRLGVVLLIATTALAGRAERAAADEPTDEPTLL
                     TYLEVDQFEYRAGNGEDTFSWEAQGWIGGDYDKVWFKTRGDDVVNGAMERAEMQLLYS
                     RAIAAFWDLQVGARYDVKPDPSRDYVVLGVQGLAPYFFEVDAAAFLSNKGDASARLEA
                     EYELLITQRLIAKPSAELNFAVQDVEELGIGSGLSEAGLELRLRYEIVREIAPYIGIS
                     WERKVGPTADIARRAGADVDDLTFVTGVRFWF"
     gene            1514..>2598
                     /locus_tag="AC16BC_10960"
     CDS             1514..>2598
                     /locus_tag="AC16BC_10960"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR3242180.1"
                     /translation="MRRAAEYYRAKFGRLPRGMWPAEGAVSSSVVDLFAEAGIAWIAT
                     DRGVLQRSGRWGYRVDDPNVLCRPYRAGEGERRLSVFSRDTTLSDRIGFHYQSYADAE
                     QAAADFIAEIKARFAHRVDDPPNRVLSVILDGENAWSAYRESARPFLHALYRRLGVDP
                     EIRTVTFAEFLEGNPARRVVSHPASSHERVYDLFCGSWIGEMGSRSGVELGTWIGEPE
                     ENRAWELLREARQTLSERAATPERHPAAYESLYAAEGSDWFWWYGDDQSADHGSEFDE
                     LFRTHVKQIYCGLGRRVPSRLSQPLVPRTVIWSFEHPVGQIERSDRLAIQTHCRGVLT
                     WAAEDAEVPQRAELTAVGGVMAGVRRYR"
BASE COUNT          474 a          785 c          894 g          445 t
ORIGIN      
        1 ggctcccatg ccggggatgg atcacggagg gcagggtgac atgggcgcca tgcctggcat
       61 ggatcacggc gcgatggaaa acatgggaac ggacacgatg ccgggcatgg cccctggcga
      121 ggcgccgatg gtcatgcacg gtcccgacac ccacggcaag ggcaactcca tggtcgcgat
      181 gatgcccaga agccgcctcc acgagcccgg cgtggggctt gaggacacgg gcacgcgcgt
      241 gcttgcctac accgacctgc gagcccgcga gccctacgat caccgatcgc ccgagcgcga
      301 gatcgagctg catctcaccg gcaacatgga tcgctacatc tggggcttca acgggaggaa
      361 gtattcggag tccgagccga tcccgatgcg gtacggcgaa cggctgcgcg tggtcttggt
      421 caacgacacc atgatggagc acccgcttca cctgcacggg atgtggatgg agctcgacaa
      481 cgggaacggc gaccgtaacc cgcggaagtt cacgatcaac gtgaaaccgg gcgagcgcct
      541 gtcgttcctc atgaccgccg acgccctggg cgcgtggccg ttccattgcc acctgggcta
      601 ccacatggcc gccggcatga tggcgcacgt ggtggtctcc gagcacggca cggagacgca
      661 ctaatgacaa acgcgaacgc cgtgatcacc ggaatcgtgc ggctgggagt cgtgctgctt
      721 attgccacga ccgcgctggc cgggcgagcc gaacgcgcgg cagcggacga accaacggac
      781 gaacccacgc tcctgacata tctcgaagtg gaccaattcg aataccgggc cgggaatgga
      841 gaggacacgt tcagttggga agcgcaaggc tggatcggcg gagactacga caaagtctgg
      901 ttcaaaacac ggggcgacga cgtggtcaat ggcgccatgg agcgtgccga gatgcagctc
      961 ctttacagcc gggccatcgc cgcgttttgg gacctgcagg tgggggcgcg ctacgacgtg
     1021 aagcccgacc cctcgcgcga ctacgttgtc ctcggggtcc aagggctggc gccctatttc
     1081 ttcgaggtgg atgcagcggc gtttctcagc aacaaaggag acgcgtcggc gcgcctggag
     1141 gccgagtacg aactgctcat cacgcagcgg ctgatcgcca aaccgtccgc ggaactcaac
     1201 tttgccgttc aggacgtcga ggaactgggt attgggtccg gcttgagcga ggccgggttg
     1261 gagttgcgac tgcggtacga gatcgtgcgc gagatcgccc cgtacatcgg catctcctgg
     1321 gaacgcaagg tcggtcccac ggctgacatc gcgcgacgcg ccggcgcgga cgtcgacgat
     1381 ctgaccttcg ttaccggcgt ccgcttctgg ttttgagtca cgtgttggga gcgtagatgc
     1441 agatctggca ctccaccgtc gatgcccaac gcactccgcg cggttttcct atcccgagga
     1501 cgcccgagcg caggtgcggc gcgcggccga gtattaccgg gcgaagttcg gtcggcttcc
     1561 cagaggaatg tggccggcgg aaggcgccgt aagttcctcc gtggtcgatc tctttgcgga
     1621 ggccgggatc gcgtggatcg ccaccgaccg cggagtgctt cagcgctcag ggcggtgggg
     1681 ataccgggtc gatgatccca acgtgctctg ccggccctac cgagccggag aaggtgaacg
     1741 caggctctcg gtgttctccc gggacaccac gctgtctgac cggatcggat ttcactacca
     1801 gagctacgcc gacgccgaac aagcggcggc tgatttcatc gcggagatca aggctcggtt
     1861 cgcgcatcga gtcgatgatc cgccgaatcg ggttctgtcc gtcattctcg atggggagaa
     1921 cgcgtggagc gcctatcgcg agtcggcgcg gcccttcctg cacgcgctct atcggcggct
     1981 cggcgtcgat cccgaaatca gaacggtgac cttcgccgag tttctggaag gaaatcccgc
     2041 gcggcgcgtg gtctcgcatc ccgcgtcgtc gcacgagagg gtctacgacc tgttctgcgg
     2101 gagctggatc ggcgagatgg gctctcgctc cggcgtggag ttggggacgt ggatcgggga
     2161 accggaagaa aaccgggcgt gggaactcct ccgtgaagcc cggcagaccc tgtctgaacg
     2221 agccgcaacg ccggagcgcc atccggccgc gtacgagtca ctctacgctg ctgaagggtc
     2281 cgattggttc tggtggtatg gagacgacca gagcgccgat catgggagcg agttcgacga
     2341 gctgttcaga acgcacgtga agcagatcta ttgcgggctg ggccgcaggg tgccgagtcg
     2401 cttgtcgcag cctctggttc cacgaaccgt gatctggtcg ttcgagcatc cggttgggca
     2461 gattgagcgg tccgaccggt tagcaattca gacccattgc cggggcgtgc tgacgtgggc
     2521 ggctgaagat gcggaggttc cacagcgtgc ggaactcacc gcggtcggtg gcgtcatggc
     2581 gggcgtccgc cggtatcg
//
LOCUS       DBNGTT010000480         3413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_369423, whole genome shotgun sequence.
ACCESSION   DBNGTT010000480 DBNGTT010000000
VERSION     DBNGTT010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_369423"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="AC16BC_10965"
     CDS             complement(<1..831)
                     /locus_tag="AC16BC_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242181.1"
                     /translation="MYDGRGVRVVTGYSGPVLASIVITPSAIVGGETAQGVVTLTAPA
                     GSGGATVRLVPSALVQALRRHTAGSVERELLRADSSGGGEHGRHHRGDVVRDRPVAAA
                     HGAASRRGRLEPRSAHGHRRQLRHGRRIPERLGACGRAGGGDDELGPLIATVPPNVTV
                     PAGAAATTFPVGTPVRDDTAAAVVSAAANGVTQSATLTVLRCTPAPATPQFAAGEWVW
                     FDDAPPAGAATHGNWVWDSAVRASGSVSLVDTAVAGIHDQWFDLATNRLTPLASDQLF
                     V"
     gene            complement(2064..2777)
                     /locus_tag="AC16BC_10970"
     CDS             complement(2064..2777)
                     /locus_tag="AC16BC_10970"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3242182.1"
                     /translation="MAKLMNETVLEKLRQCADTIEPVARGAWLHAISDLGRFGLTTDA
                     DLHVALSDDADAAKQAAVCWALGHLKDERAVDGLVRAMERNNEVAFEAANALVRIGSK
                     RAVASLVTLLRSSAPLPRRAAAAYALGYLGGQSGNALDDVLGDRRQPASLRARCAEAL
                     GVMRWRAALESLMSATYDPSPEVRFWSIYALGELGDPMARSTVAARLHDAEEVATLGS
                     IGAEATRVLEWLDAHNTSA"
     gene            complement(2765..>3413)
                     /locus_tag="AC16BC_10975"
     CDS             complement(2765..>3413)
                     /locus_tag="AC16BC_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HIR3242183.1"
                     /translation="HTLRAGADRHQPLSLPGQEADREGERSYNIFRWYRAGWGRYTSV
                     DRFGWDSVAGSAAVRERWPDYLLSPEMGVAYSYALNSPLARTDPRGESAAAALPLAGA
                     CALLDGPLPFGDILAVAILAGAAIGAINNVDFNKCDDCEEKKKKGRWTCVATCNVVPF
                     APKPGITYPDRVQGTGNGNDRGTACQAAISVTQSMSPFGSYTRHCHCKKQDCWRN"
BASE COUNT          681 a         1094 c          992 g          646 t
ORIGIN      
        1 gacgaacagt tggtccgagg ccagcggtgt caggcggttc gtggcgaggt cgaaccattg
       61 atcgtggatg ccggcgaccg ccgtatcgac gagcgacacc gagccgctgg ctctgaccgc
      121 ggagtcccag acccagttgc cgtgcgtggc ggctccggcg ggcggggcgt cgtcgaacca
      181 gacccactcg cccgccgcga actgcggcgt tgcgggcgcg ggagtgcagc ggagcacggt
      241 cagcgtcgcg gactgcgtca cgccgttggc cgccgcggac accaccgcgg cggctgtgtc
      301 gtcgcgaacg ggcgtgccca cggggaacgt cgtcgcagcc gctcccgccg gcacggtgac
      361 gttcggcgga acggtggcga tcaggggtcc gagctcgtcg tcgccaccgc cagcccgccc
      421 gcaggcgccg agccgctcag ggatacggcg gccgtggcgg agctgccgcc gatgaccgtg
      481 ggcggatcga ggctcaagcc tgccacgacg ggaggcagca ccgtgagcgg cggcgacagg
      541 acggtcccgc acaacgtcgc cgcgatggtg acgtccgtgc tcgccgccac cgctgctgtc
      601 tgcgcggaga agctcgcgct cgacgctccc tgcggtatga cgacggagtg cctgcacgag
      661 tgccgaggga acgagccgca cggtcgcgcc cccgctgccg gccggcgcgg tcagcgtcac
      721 cacgccctgc gccgtttccc cgccgacgat ggcggacggt gtgatcacga tcgacgcgag
      781 caccggtccg gaatacccgg tcaccacgcg cacgccgcgg ccgtcgtaca cgtaggactc
      841 gtctcccgac gacgtcagat gattcgcagg cgagtagctg gacgcctcgc ctccgacgcc
      901 gatctcattg cccgccgcgt cctggtagac ggcgcgcgtg ccgttctccg tgaccgacgc
      961 aagcttcggc gtaccggcaa cgtaacgtgc ggaaacccgt ggtttaccta aagctgaaaa
     1021 tcggctgttc cgtactcggc atcgcgaaga acgataacgc tatatcgtgc gttggggaat
     1081 agagtcgtag caggcgtagt cgcttcatac ccggctggtg ctgcgccgta cgttatctga
     1141 gcaacgggag caaaccggct catcaaatcc tcatcggatt tcaacactcc gcggaaaaga
     1201 gtcccgaacg catgcggcag cggaacggaa acaaacgcgc cagcgccgtc gccgctcggc
     1261 tgcatcgatg atgggtgaac ttcccatatt gtgtctccct gatgggtaac aaccacgaga
     1321 gtaacgaacg attggggccc gtcgtcaaaa acaaaaagga cttgccccga ggtgtccctc
     1381 gtcaccgtga tatcaatggt gcgcatctta gaattcacct ggcggcattg tcgggtctgg
     1441 attcatcggt ttttgtttga tgggcatcgg cggaaaacat ttccagccat gtaggtcttg
     1501 agtgagttga ttgccctcca ttgtccaagt gcatataccc gagcactttt tgcaaagctt
     1561 tccggaggga aggggcagcg gctcaacgcc tccaagcgtc ggatagatct gtttcgaaat
     1621 ggtgccgata cagaagccac cataaatgca ttcgccataa atcggcatag gccacaacgg
     1681 cttcgccaca caccagactt tctcaagtcc gaggggatcc ataaacgata ttggatttcc
     1741 gccaacgtat gcttaagagt ttagttcggc acccgcgtcg aatgagagac tctgtccgat
     1801 ctcaacgccc agacgcaaga gtccaacctg agctacgttc agaattaccc accataaagc
     1861 ggtcagcggc aacccaaata ctgccaggat gacgctccac atacatgccg actcgcttcg
     1921 cagggtggca ccgtgatgtg gccactatca gctatcgccc aggccatagt cgatagacac
     1981 agaagcaacc aaatgattct cagatggttg atcatttgac accgcatttc gctcggcatc
     2041 gctcattgac accgtcatca tattcaagca gaagtgttgt gcgcgtctag ccattcgagc
     2101 acccgtgtcg cctcggctcc gatcgatccc aatgttgcca cttcttcagc atcgtgcagc
     2161 cgcgccgcga ccgtggacct cgccatggga tcgcccaact caccgagtgc gtagatggac
     2221 cagaaccgaa cctctggaga tggatcgtaa gtcgccgaca tgagcgattc gagcgcggct
     2281 cgccatcgca ttaccccaag cgcttcggcg catctggctc gcagactcgc aggttgacgc
     2341 cgatcgccca gaacatcatc aagagcattt ccgctttggc cgccaagata gccaagcgcg
     2401 tacgccgccg cagcgcgtct cggcagcggt gctgatgatc tgagcaacgt gactagcgac
     2461 gcgacggctc gtttgctccc gattcggacg agagcattcg ccgcttcgaa cgcaacctca
     2521 ttgttacgtt ccattgctcg aaccaatcca tccactgctc gctcatcttt caaatgacca
     2581 agtgcccagc acaccgcggc ttgtttcgct gcatcggcgt cgtccgacaa tgcgacgtga
     2641 agatctgcat cggtcgtcaa cccgaagcga cccagatcac tgatcgcgtg cagccatgca
     2701 ccccgtgcga ccggttcgat tgtgtcggca cactgtcgta gtttttcgag cacagtttcg
     2761 ttcattagtt tcgccaacag tcctgcttct tgcagtgaca gtgccgtgtg tatgacccga
     2821 acggcgacat actctgtgtc acactaatcg cagcctgaca agctgtcccc cggtcgttac
     2881 cgttccctgt gccttgaact cgatccggat atgtgatccc cggctttggt gcgaacggca
     2941 cgacattgca agtcgcaacg caagtccagc gacccttttt cttcttttcc tcgcagtcat
     3001 cgcacttgtt gaaatcgaca ttgttgattg caccaatggc cgcacctgca agaatggcca
     3061 ctgcgagaat atccccgaac ggaagcgggc catccagaag agcgcacgct ccagcgagcg
     3121 gcagggctgc cgctgccgat tctccgcgcg gatccgtacg cgctaacggt gaattgagag
     3181 cgtagctgta cgccacgccc atttccggac tcaaaaggta gtccggccat cgttcacgga
     3241 ccgcagccga tccggccact gaatcccagc cgaagcgatc aacactcgta tatcgccccc
     3301 accccgcccg gtaccagcgg aagatgttgt agctccgctc cccttcccga tccgcctcct
     3361 ggccgggcaa gctcaggggt tggtggcgat cggcgccggc gcggagggtg tgg
//
LOCUS       DBNGTT010000481         8451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1776479, whole genome shotgun sequence.
ACCESSION   DBNGTT010000481 DBNGTT010000000
VERSION     DBNGTT010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1776479"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="AC16BC_10980"
     CDS             complement(<1..1047)
                     /locus_tag="AC16BC_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3242184.1"
                     /translation="MGLEPGAIILADLNGDIVPDLVTANEGSNDISVLLSNGDGTLAT
                     QASYGVGIHPIGVVIADLNGDNILDVVTANRGSNDMSVLLGYGDGTFTSQASYPVNST
                     PRAITIADFNGDTVPDVITVNSRDDVSVLLGLGNGTFASQVAYGTGATPIAVVVADLN
                     GDQMPDLVTANVNSSDVSVLLGHGDGTFTDRMSYSVDANPVSITAADLNKDGFPDLIT
                     ANTNSCYATGGPNDVSVLLGHGDGTFAGQIVYGTGVCPIAVAIADLNGDTVPDIVTVN
                     QSSGDVSVLLGQSDGMFVSQATYAVGSQPSSIVIADLNGDLIPDLVTSNTGSKNASVL
                     LGHGDGTFAARAIYY"
     gene            complement(1340..1879)
                     /locus_tag="AC16BC_10985"
     CDS             complement(1340..1879)
                     /locus_tag="AC16BC_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR3242185.1"
                     /translation="MAQAVNTILAALTVASHAVLLLGLAYFLIHRNDPANVILTFTGR
                     HALLFAWLVSCAATALSLLYSEVIGYEPCVLCWYQRIFIYPQVVLLGLAWWRQDDNII
                     DYSLLLALIGAVIALYHTYIGYGGAPLLACGADESSNPCAKRYVFEFGYVTIPVMSLT
                     SFLLVITLLGVRKFYQGSR"
     gene            complement(1882..2244)
                     /locus_tag="AC16BC_10990"
     CDS             complement(1882..2244)
                     /locus_tag="AC16BC_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242186.1"
                     /translation="MVIRAIVGLVAAVVIVLGVHEYGKPGKYDAFAQCLTDKGATFYG
                     AFWCPHCQTQKRMFGKSAKLLPYVECSTPDGKGKLAICTEKGVESYPTWEFPDGTRKS
                     GEVPLAELAIKTDCPLPQ"
     gene            2415..2948
                     /locus_tag="AC16BC_10995"
     CDS             2415..2948
                     /locus_tag="AC16BC_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RipA family octameric membrane protein"
                     /protein_id="HIR3242187.1"
                     /translation="MNTEIDSAGDLRETYRNLVELWTSGVRDYHTLLSDYFTANSVFV
                     AAIGFLLVREPLSAIFLLLIVILCGFGILMALQMAIVLGRFSAQTALWEWRLRGIEHT
                     AIWTQQRLFVDLQRLRDRHQALEDADNEPPVMAPNWATRRHRQWWARREISFPLFFGI
                     VYTLFLAWGVTQIFAGS"
     gene            complement(2970..3434)
                     /locus_tag="AC16BC_11000"
     CDS             complement(2970..3434)
                     /locus_tag="AC16BC_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242188.1"
                     /translation="MQTKRWLLQYGVAVGLAALFAAMLGQIPLFRETMVGKFRASNLV
                     QFIGYGAALITILLGARQIAGDRSDAWKWLGPFRDLVVPAALLVVLPLAYQVALLALG
                     PLLGAGGKRVYNWMFVLGIAGAGGWVIATWVTKCAPKFADAGTEHKEEKRAA"
     gene            complement(3567..6005)
                     /locus_tag="AC16BC_11005"
     CDS             complement(3567..6005)
                     /locus_tag="AC16BC_11005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3242189.1"
                     /translation="MRPIDQIVQLLSHQAIAFLLGWTAAYLAAARIRKSHWLVSIAVV
                     SGLQALAWWILAQFTPGPSPAWLAAPAAVGLLTIALTEHWNALAHACMTSSITVSVLF
                     LAYVVAVTVHANLGPMSLAFAGILMAMQAITLVLMVASSYEILDVMGRVRWTRVRHPR
                     PTTDYFPKVSLHVPAYNEPPDMVKETLDALARLDYPNYEVIVIDDNTADERLWRPIEA
                     HCKTLGFKFFHLDNWPGYKSGALNFALTRTAADAEVIGVVDSDYVVEPNYLKDCVGFF
                     KDPNLAFVQTPQDYRDVAPDDRYATACYHSYLYFFKVSMASRNEHNGIIFAGTMGLIR
                     RSVLQELGGWDEWCITEDAEISLRILDRGYESVYLDKSYGHGLMPLNFEGLKKQRFRW
                     AFGGMQILKAHWRSLAPWAGRDESHRLTGAQRWDYLLGGLQWLNDPLTFGFTVLLLLG
                     ATALVIGRSLFIQPMIGAVIITPFLFIFIGVTRFLWVLRHRVGCTWDRALSAFAILLS
                     LTWVVTLACILGLVKREGAFLRTPKKRGAADPWHAARIVSHETLLAGLCVVAAVALAW
                     KVPPSPATTVMIGLLVWQTVVYAAAPVASLWSFRSETRASHPAYAAASSRTTGLRISS
                     MIEDRALARGAFALALALCAFFYFAVTLAPEREQLFRANPSETPLVGPALITASPVAQ
                     VKSVLFIEERTALHADVDRAVRLWDPRGVIRDANYTPDDPSDDRTWTGLDGIRGRYRT
                     EFAARRYLSLAHTDASVIIENDRAVVVNDLRAEIQTASGVQTVFLSKGDRWTFIRGPD
                     GWKIAELVVNRAPR"
     gene            complement(6100..6621)
                     /locus_tag="AC16BC_11010"
     CDS             complement(6100..6621)
                     /locus_tag="AC16BC_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR3242190.1"
                     /translation="MRPSTRSHTTYQSIAAVLLILGAAMLFPAGALAKSTKFDGLVMR
                     VHDGDTLSVVRRGRAEKVKLAGIDCPELRQSFGSSARNFTSRLTSGQTVTVRIQGTDE
                     QGRILGVVILPDGRSLNRELVAAGLAWKLKDSKNKQVAQLEKDARRAKRGLWADAHPT
                     PPWDFRKTKSAKR"
     gene            complement(6638..6904)
                     /locus_tag="AC16BC_11015"
     CDS             complement(6638..6904)
                     /locus_tag="AC16BC_11015"
                     /inference="COORDINATES: protein motif:HMM:NF022603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HIR3242191.1"
                     /translation="MKQNRHGLTSVTTTTQPGSHDPAVQQRIAERAYQLFQQRGGSHG
                     SDVQDWLTAEREVMAATRGRRSRNARNHPANGHSPTRGKTRRAA"
     gene            7230..7391
                     /locus_tag="AC16BC_11020"
     CDS             7230..7391
                     /locus_tag="AC16BC_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3242192.1"
                     /translation="MPMYEYECEKCKKTFTLALSFKEHEEGAVCPECGSKKVTQLISS
                     FIAKTASKT"
     gene            7540..7842
                     /locus_tag="AC16BC_11025"
     CDS             7540..7842
                     /locus_tag="AC16BC_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR3242193.1"
                     /translation="MATYVMLTKVSPESMASPAKLRQYEEDVKAKIKRHCPQVSWVSN
                     YAVLGPYDYLDVFEAPDEVMAGKVATIVRSFGHATTETWGAIPWARFKSMVDDLAA"
     gene            7894..>8451
                     /locus_tag="AC16BC_11030"
     CDS             7894..>8451
                     /locus_tag="AC16BC_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3242194.1"
                     /translation="MTVIIERRAFDDLFTALGERGYTLVGPTIRDSAIVYDRIRSPTD
                     LPVGWTDEQSGGVYRLTRRDDHALFGYAVGPQSWKKYVLPPRLTLWRAQREADGFVVI
                     EPEHDPPRLALIGVRACELRAIAIQDRVLMEGECVDPHYRAQRERLFVLAVNCGHAAG
                     TCFCVSMQAGPRASGGFDLALTEIID"
BASE COUNT         1580 a         2511 c         2797 g         1563 t
ORIGIN      
        1 ataatagatt gcccgggcag cgaaggtccc atcgccgtgg cccagtaaca ccgatgcatt
       61 cttggagccc gtgttggacg taacaaggtc ggggatgaga tccccattaa ggtcggcgat
      121 aacgatagaa ctaggctggg aacctacggc ataggtggcc tgggaaacaa acattccgtc
      181 gctttggcct agcaataccg acacatcacc ggaggactgg ttcaccgtga ctatgtctgg
      241 tacggtgtcg ccgttgagat cggctatggc gaccgcgata gggcagacgc cagtcccata
      301 gacgatctgg cccgcgaagg ttccgtcacc gtggcccagc aacactgaca catcattcgg
      361 gccaccggtt gcataacagg aatttgtatt ggctgtgatc aggtcgggga acccgtcttt
      421 attgagatct gcggccgtaa ttgaaaccgg gttggcatcc acggaatagc tcatcctgtc
      481 ggtaaatgtg ccgtctccgt ggcccagcaa taccgacacg tccgaggagt tcacattggc
      541 cgtgaccaag tcgggcattt ggtcgccgtt gaggtccgct acgacgaccg cgataggggt
      601 ggcgccggtt ccataggcca cctgggaggc gaaggtcccg ttgccgagac ccagcagcac
      661 tgaaacatca tcacgggagt taaccgtaat tacgtcgggc acggtgtcgc cattgaaatc
      721 ggcgatcgta attgctctcg gcgtggaatt aacgggatag gaggcttgag aggtgaaggt
      781 cccgtcgcca tatcccagca ataccgacat gtcgttggac cctcggttcg ccgtgaccac
      841 atccagtatg ttgtcaccat tgaggtctgc tatgacgact cctatagggt gaatgcccac
      901 tccataagat gcctgggttg cgagcgtccc gtcgccgttg ctcaacaata ccgagatatc
      961 attggacccc tcattggccg tgaccaggtc gggtacgatg tcgccgttga ggtcggccag
     1021 gatgatggcc ccgggttcaa gtcccacaga atagagttca ttgggaaacc gcgcagctgc
     1081 tggatactcg acgtataggg gcgcaggtga tggtgtgcct tcaccgctgc cacaggcggc
     1141 cagggatccg tttaggacaa gcagtgcaag aacatgcttc caggacggcc tgtgatccat
     1201 gatccgttgc tctgtgtttc cgtgttggac cggttgagaa gaaacgacca gacggcgtct
     1261 ttattagact agacagcttt cagggggcat ttcaagcaaa aaagtcgatc ggtttgccgt
     1321 cgcgaatgtg cggtattgac tatcgactgc cttggtagaa cttccgaacg ccgagcagcg
     1381 tgatgaccaa caggaatgag gtgagcgaca tcactggtat cgtgacgtag ccgaattcga
     1441 agacgtagcg cttggcgcag gggttgctcg attcgtccgc gccgcaggcg aggagcgggg
     1501 cgccgccgta gccgatgtag gtgtgataga gcgcgatgac tgcgccgatc agggcgagca
     1561 gcaggctgta gtcgatgatg ttgtcgtctt gtcgccacca tgccagcccc agcagcacga
     1621 cctgcgggta gatgaagatg cgttgatacc agcagagcac gcagggctcg tatccgatca
     1681 cctcggaata gagcaggctc aacgcggtgg ccgcgcacga gaccagccac gcgaacagca
     1741 gggcgtgacg ccccgtgaaa gtcaggatga cgttcgcggg atcgtttcga tggatcagga
     1801 aataggcgag cccgagcagc aggacggcgt ggcttgcgac cgtgagcgcg gccaggatgg
     1861 tgttgacggc ctgcgccatg gttactgggg cagggggcag tcggtcttga tcgcgagttc
     1921 agcgaggggc acttctccgg acttgcgggt gccgtccggg aattcccagg tcggataact
     1981 ctccacgccc ttctccgtgc agatggccag tttgcctttg ccgtcgggcg tggagcattc
     2041 cacgtagggg aggagtttgg cggatttgcc gaacatccgt ttctgggtct ggcaatgcgg
     2101 gcaccagaac gcgccataga acgtggcgcc cttgtccgtg aggcattggg cgaacgcgtc
     2161 gtatttgccc ggcttgccgt attcgtgcac gcccaggaca atgaccacgg ccgcgaccaa
     2221 tcccacgatt gctctgatca ccacggatct ggtgcgagag cgggacgtcc gtggggttga
     2281 ttgtgcgcgc gcagccatcg ccgcattata ccaaggtttc agagggcaag gcgtgcgccc
     2341 gcgccatgcg ccgacgaaag tcttgcgttt cggtgtatga tggccgcgtt tcgtggagtg
     2401 gtggccggat gccgatgaat accgagatcg actcggcggg agatctgagg gagacatacc
     2461 gcaatctcgt cgagctctgg acttcgggcg tgcgcgacta ccacactctg ttgtccgact
     2521 acttcaccgc gaactccgtg ttcgtggccg ccatcggctt cttgctggtc cgcgagcccc
     2581 tcagcgccat ctttctgctg ctgatcgtga ttctctgcgg gttcgggatt ctgatggcgt
     2641 tgcagatggc gatcgtcttg ggtcggtttt cggcccagac cgcgctctgg gagtggcggc
     2701 ttcgcgggat cgagcacacc gcgatctgga cgcagcagcg gttgttcgtg gatcttcagc
     2761 gcttgcggga ccgccaccag gcgctcgaag atgcggacaa cgagccgccc gtgatggcgc
     2821 ccaattgggc gacgcgccgg catcggcagt ggtgggcgcg gcgggaaatc agcttcccgc
     2881 tctttttcgg catcgtgtac acgctctttc tggcctgggg cgtgacccag attttcgccg
     2941 gatcgtgaag ccccgggcgg tccgaaggat caggccgccc gcttttcttc cttgtgctcg
     3001 gtcccggcgt cggcaaactt cggcgcgcat ttggtcaccc acgtcgcgat gacccacccc
     3061 ccggcgcccg cgatgccgag cacgaacatc cagttataaa cccgcttccc tcccgctccg
     3121 agcaacggac cgagcgccaa tagcgcgacc tgataggcca acggcaacac caccaacagg
     3181 gcggccggca ccaccaggtc gcggaatggg ccgagccatt tccaggcgtc cgaacgatct
     3241 ccggcgattt ggcgggcgcc gagcaggatc gtgatcaagg cggcgccgta cccgatgaac
     3301 tgcacgagat tcgacgctcg aaacttcccg accattgttt ctcgaaacag cgggatctgt
     3361 ccgagcatcg ccgcgaacaa tgccgccaat ccaacggcca caccgtattg gagcaaccaa
     3421 cgttttgttt gcattgcgcg cctcccgtgc gttcgctacg tctttgaacc gtgcggagcc
     3481 ggaccgggtc gacgtgactc cgccaaacgc agggaagcct aacacgcaac agatgttttg
     3541 tcgtccggct ggaggtttcc gacccgtcac cgcggggcgc gattgaccac gagttcagcg
     3601 atcttccagc cgtccgggcc gcggatgaag gtccaccgat cgcctttgga gaggaacacg
     3661 gtctggactc ccgacgcggt ctggatttcc gcgcgcaagt cattgaccac cacggcgcgg
     3721 tcgttctcga tgatgaccga ggcgtcggtg tgcgcgagcg agagataccg tcgggccgcg
     3781 aattcggtcc ggtatcgccc gcggattccg tccaggccgg tccacgtgcg gtcgtcgctc
     3841 gggtcgtccg gcgtgtagtt ggcgtcgcgg atgacgcccc gcggatccca gagccgcacg
     3901 gcgcggtcca cgtccgcgtg cagggcggtc cgctcctcaa tgaacaagac ggatttgacc
     3961 tgcgcgaccg gcgaggcggt gatcaacgcg ggaccgacca acggggtttc ggagggattc
     4021 gcccggaaca attgctcgcg ttccggagcc aaggtgacgg cgaaatagaa gaacgcacag
     4081 agtgccaggg ccaacgcgaa cgcgccgcga gcgagtgcgc ggtcctcgat catggacgaa
     4141 atacgaaggc cggtggtccg ggaggacgcg gcggcgtacg ccgggtgcga ggcgcgggtc
     4201 tcgctgcgaa agctccacag gctcgcaacg ggcgcggcgg cgtacaccac ggtctgccag
     4261 accaacaggc cgatcatgac cgttgtggcc ggggagggcg gaaccttcca cgccaatgcc
     4321 acggccgcga ccacgcacag gccggccagg agggtctcgt gcgagacgat ccgcgccgcg
     4381 tgccatgggt ccgccgcgcc gcgttttttg ggcgtgcgaa ggaaggcgcc ctcgcgtttg
     4441 accaagccca agatgcacgc gagcgtcacg acccacgtca aactcagcaa gatggcgaac
     4501 gcggacaggg cgcggtccca ggtgcagccg acgcgatgcc gcagcaccca gagaaaccgc
     4561 gtgacgccga tgaagatgaa cagaaacggc gtgatgatca cggcgccgat catgggttgg
     4621 atgaagaggg atcggccgat caccagcgcg gtcgcgccca gcaacagcag gaccgtgaac
     4681 ccgaacgtca gcgggtcgtt gagccactgc agccctccga gaagatagtc ccatcgctgc
     4741 gcgccggtga ggcggtgcga ttcgtcgcgg ccggcccacg gcgccagcga tcgccaatgg
     4801 gccttgagaa tctgcatgcc gccgaaggcc cagcggaacc gttgcttctt gaggccctcg
     4861 aagttcaagg gcatgagtcc gtgaccgtag gacttgtcga ggtacaccga ctcatacccc
     4921 cgatccagaa tccgcaaaga gatttccgcg tcctcggtga tgcaccactc gtcccatccg
     4981 cccagttctt ggagcacgga tcgccggatc aatcccatgg tgcccgcgaa aatgatgccg
     5041 ttgtgctcgt tgcgggaggc catcgacacc ttgaagaagt acagatacga gtggtagcag
     5101 gccgtggcgt accgatcgtc cggcgccacg tcgcggtagt cttgcggcgt ttgcacgaac
     5161 gcgaggttcg ggtccttgaa aaagcccacg cagtccttga ggtagttcgg ttcgaccacg
     5221 tagtcggagt ccaccacgcc gatgacctcg gcgtcggccg cggtgcgggt gagggcgaag
     5281 ttgagcgcgc cggacttgta ccctggccag ttgtccaggt gaaagaattt gaatccgagg
     5341 gtcttgcagt gcgcttcgat cggccgccac agccgctcgt cggcggtgtt gtcgtcgatc
     5401 acgatcactt cgtaattcgg gtagtcgagc cgcgcgagcg cgtccaacgt ttccttgacc
     5461 atgtcgggcg gttcattgta cgcgggcacg tgcagggaca ccttgggaaa gtagtccgtc
     5521 gtcggtcggg gatggcgcac ccgcgtccag cgcacgcgac ccatcacatc gaggatctcg
     5581 taactgctcg ccaccatgag cacgagcgtg atcgcctgca tcgccatgag gatgccggcg
     5641 aacgcgagac tcatggggcc gagattggcg tggaccgtga cggcgacgac gtacgccaga
     5701 aagaggacgc tcaccgtgat gctgctcgtc atacaggcgt gggcgagcgc gttccagtgc
     5761 tcggtgagcg cgatggtcag gaggccgacg gcggcgggcg ccgccagcca ggcgggcgac
     5821 ggtcccgggg tgaattgggc gaggatccac caggcaaggg cttggagccc actgaccacg
     5881 gcgatcgaga cgagccaatg cgacttgcgg atcctggcag cggccagata ggccgccgtc
     5941 caacccagga ggaacgcaat ggcttggtgg gagagaagtt ggacgatctg gtcgatcgga
     6001 cgcatcgccg ccctcggcgg taagcggaac tgagcctctg ggcaacggac ccggttcgcg
     6061 ggaccaatat tcccggaccc acggagcccg ggaagagggc tagcgcttgg cgcttttggt
     6121 cttccgaaag tcccagggcg gcgtggggtg ggcgtcggcc cagaggccgc gtttggcgcg
     6181 gcgggcgtct ttttcgagct gagcgacctg cttgtttttc gagtctttga gcttccacgc
     6241 cagcccggcg gccacgagtt ctcgattcaa tgatcggccg tcaggcagga tcaccacccc
     6301 gaggatgcgg ccctgttcat ccgtgccttg aatgcggacc gtgacggttt gtccggacgt
     6361 gaggcgcgag gtgaagttcc tggcgctgga gccgaaggat tggcgcagct cggggcagtc
     6421 gatcccggcg agtttgacct tttcggcccg gccgcggcgg accaccgaca gggtgtcgcc
     6481 gtcgtggacc cgcatcacca agccgtcgaa cttggttgat ttggcgagcg ccccggccgg
     6541 gaacagcatt gcggcgccca ggatcagaag aaccgcggcg attgattgat acgtggtgtg
     6601 agaacgggtg cttggacgca tgggcgtctc cttcgggtta cgcggccctg cgggttttcc
     6661 cccgcgtcgg ggagtgcccg ttggccgggt gattccttgc gttcctgctc cgccgaccgc
     6721 gcgttgcggc catgacctcg cgttccgcgg tgagccaatc ctgcacgtcg cttccgtggg
     6781 agccgccccg ctgttgaaag agttgatacg cgcgctccgc gatccgctgt tgcaccgccg
     6841 ggtcatgcga gccgggctgc gtggttgtcg tgacgctggt caggccgtgt cggttctgtt
     6901 tcatggatcg atcctccgtg atggtcccaa ctgtgagtag atacgaactg ctcgccccgt
     6961 gggggtaaat gtagcactcc tccgccgaaa caccatgtga agacggggac cccggccggt
     7021 cagtgcccgg caatccaaac gaggtacggt ctctccccag tttgtccggc cgtatagggc
     7081 gagcgcggga tggaggcgga gacgcgactt gcgcgatact tgcttcgcgg aacgccatcg
     7141 gcatataaag aaaccgttct cgggctgttc ccgccttcgc agaggtagcg acgacgtttt
     7201 attgaccggt cgtggcagga ggattcgtca tgccgatgta cgaatacgaa tgtgagaagt
     7261 gtaagaagac gttcacgctc gcgctcagct tcaaggaaca tgaagagggc gccgtgtgtc
     7321 cggagtgcgg gtccaaaaag gtcacccaat tgatcagttc gttcatcgcc aagaccgcca
     7381 gcaagacgta aacaacgcgt cacgccgcat gcgacacgcg ttgttcttcc accggagaac
     7441 gggccgggga gtccccggcg gcgccgagac atggcgcgga gttgctcgcg cgtcgcggcg
     7501 cgacgtgtaa tgggacgatc tcacaccagg aggagcacca tggctaccta tgtgatgctg
     7561 acaaaggtct ctccggaaag catggcctcg ccggccaagc tcagacagta cgaagaagac
     7621 gtcaaggcca aaatcaaacg acactgcccg caggtctcgt gggtcagcaa ctacgcggtg
     7681 ctgggtccgt acgactatct ggatgtgttt gaggcgcccg acgaggtcat ggcgggaaag
     7741 gttgcgacga tcgtccgctc cttcggtcac gccaccacgg aaacctgggg cgcgattccc
     7801 tgggctcggt tcaagtcaat ggtcgacgat ctggcggcgt aacggagcac cgggcatgtc
     7861 atcccgcggt cgttcggtgg agcactgccg cccatgaccg tcatcatcga acgacgggcg
     7921 ttcgacgacc tgttcacggc gttgggcgag cgcggctaca cgctggtggg gccaaccatc
     7981 cgggacagcg ccatcgtgta cgaccggatc cggtcgccca cggacctgcc ggtcggatgg
     8041 accgacgagc agagcggcgg ggtgtatcga ctcacgcgcc gcgacgacca cgcgctgttc
     8101 ggctacgcgg tcgggccgca gtcgtggaaa aaatacgtat tgcccccccg tttgacgttg
     8161 tggcgcgccc agcgcgaggc ggacggcttc gtggtcatcg agccggaaca tgaccctccg
     8221 cggctggcat tgatcggcgt gcgcgcgtgt gagttgcggg cgatcgcgat tcaggatcgc
     8281 gtgctgatgg agggggagtg cgttgatccg cattatcgcg cgcaacggga acggctgttc
     8341 gtgctcgcgg tcaactgcgg ccacgcagcg ggcacgtgtt tctgcgtgtc catgcaggcg
     8401 gggccgcgcg cgtccggcgg gtttgatctg gccctgaccg aaatcatcga c
//
LOCUS       DBNGTT010000482         9761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1776630, whole genome shotgun sequence.
ACCESSION   DBNGTT010000482 DBNGTT010000000
VERSION     DBNGTT010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1776630"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC16BC_11035"
     CDS             <1..633
                     /locus_tag="AC16BC_11035"
                     /inference="COORDINATES: protein motif:HMM:NF051347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3242195.1"
                     /translation="PQFQRALQIDPSYHQARRNLALAYQRTFRWADALAEYETLGRLA
                     DGSDDDRLLIGALALQAGDLDKAAANLHAVINRHPDSYPAWFNLGLAEVRRGRATEAE
                     QAFQQAAALNPNDPDVYYQRGVLASRDGRSSDAAQLFEAALARRPEYFLAHYELARLE
                     DGRGRRDAAAEHYQRFVETVPRDPAWSDARAEADAWLAARGRSGHGAVGR"
     gene            complement(607..1581)
                     /gene="queG"
                     /locus_tag="AC16BC_11040"
     CDS             complement(607..1581)
                     /gene="queG"
                     /locus_tag="AC16BC_11040"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR3242196.1"
                     /translation="MSTSLAELIKREALALGFAAVGIAPAAPLPEHQRAFRTYLEDGR
                     HAGMAYLAQEPERREDPARVLPGAKSVIALGWAYYAGDHPPPPDPLAGRIARYAWGPD
                     YHDVIEPRLERLKAFILAHRPDAAIRCAVDHAPVLERAYAQHAGLGFIGKHTLLITPD
                     HGSWILLGELITDIELPVDDPAVNQCGSCTRCMEACPTGALTAPFQLDAGRCIAYLTI
                     ENKNEIPAPLRPLVGDWIFGCDLCQEACPYNEAPVESSAQPEAPGRGQAGELGPWLML
                     DDAATPRSHGQYRDRFARTALRRITSKHLTRNAAVVRANQLTADRPMA"
     gene            complement(1562..2407)
                     /locus_tag="AC16BC_11045"
     CDS             complement(1562..2407)
                     /locus_tag="AC16BC_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HIR3242197.1"
                     /translation="MASAMLIKRLMRERPYLREPLQFYRRVCEIWDEATPDDLTPVLA
                     PTDERPLRLARGLPLLDPARFGSADLTHVAQTFTRLLAVVRARTPALAPVVDALDAAS
                     GAGPDRARWLARLLGGHEPPPANAPAEFVAYAVRLALRPSLAALLPALTDVFTDRAWN
                     RPLCPLCGFPPGMAEERERDRRLYCSVCPTSWIFTRPGCPFCGNAVDQHLACSIEDVE
                     PGYHVAICTECNGYLKTAHAHELGREIDPSLEDLLTVPLDHAAQRDGYRPPLAADAAG
                     VDLAR"
     gene            2532..3977
                     /gene="tldD"
                     /locus_tag="AC16BC_11050"
     CDS             2532..3977
                     /gene="tldD"
                     /locus_tag="AC16BC_11050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR3242198.1"
                     /translation="MTERALVPQRFFETRFGLTSRDLETVVARAMERRVEYADLYFEY
                     EVSEAVSLEEGLVKTASRDISQGVGVRAVAGDRTGYAYSNDISVPQIDLAAQTARSIA
                     DAGKSTSVLVPGGGPLIRDLYPVDQSVLDAPLSARIDLLQDIDRLARAYDPRIRNVMA
                     GLSCESKLVLVVTSDGAIVGDVRPLARLNVTCIAQDGSNRQIGTYGGGGRIGFDFFFD
                     QDRWRTFTREAARRAIGNLSARPAPAGTMTVVLGPGWPGILLHEAVGHGLEADFNRKG
                     VSAFSNRLGQRVASELCTIVDDGTIAGRRGSLNVDDEGSPSGRTVLIENGILRGYLQD
                     KTNARLMKMPVTGNGRRESFAHAPMPRMTNTFMLAGSSTPEEILASVDHGLYAVSFGG
                     GQVDITSGKFVFSASEAYLIEKGRVTHPVKGATLIGNGPEVLKRVSMVGNDLSLDAGV
                     GTCGKEGQSVPVGVGLPTIRIDGLTVGGTQG"
     gene            3979..5325
                     /locus_tag="AC16BC_11055"
     CDS             3979..5325
                     /locus_tag="AC16BC_11055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR3242199.1"
                     /translation="MPIERDLAEELLAKARRLGATEADVMAVEGTAFSTRVRLGEVDK
                     LEQARERRLGLRVIVDQRTAVSSTADLSPASLDDLVSETYARAVRMAPDPHAGLPAKG
                     QGIVSVPDLDLWDGAYGDPPADEKIELARAAEAAALGFDPRISNSEGGEFDYGRSEIV
                     YADSNGVYGRYRSSSFSLSVVAIAAREGAMQRDYWYSARRKFRLLDPPEAIGKVAAQR
                     ALARLGARKVKTQEAPVVFDPQMSARLVSALTTAVSGYALYKGASFLGGALGQTVASP
                     LVTIVDDGTLPTGLGSKPFDGEGLPTRRTVVVDQGILSSYLLDTYSAKKLGLAATGNA
                     ARGVGDTPSVAPTNCYLAAGASSPQDIIASVRNGLYVTELIGFGVNTVNGDYSQGASG
                     LWIENGEVTFPVEEITIAGNLKDMLRQIEMVGNDLEFRSRVCAPTVKIASMTIAGT"
     gene            complement(5497..6477)
                     /locus_tag="AC16BC_11060"
     CDS             complement(5497..6477)
                     /locus_tag="AC16BC_11060"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIR3242200.1"
                     /translation="MGAVITGTGIGLPSRIVTNEELETGLALPAGAILRRTGIDSRRW
                     VLEHEASSDLAATAARAALNAAQRLPSDVDLLLVSTTSGDMGFASTACLLQRELKAAG
                     CPAFDLAASCTGFLYGLSTAQAFLRTGQARTVLLAATEVKSRFIDQRDPTSAILFGDG
                     AGAVVLSEGPEDRGVLDVTLHADGTRWPLIHLPAGGSREPLSEASLAANRHAMHLDGS
                     AVFRTAVRTIEHAVRDQAARFDLPLDQIDHFIFHQANLRILQQVTRRLQLPDAKVPVT
                     ISRYGNTSSSALPIALHHAVAEGRIRSGEWVLLAAFGGGITWGTALIRWP"
     gene            complement(6484..6966)
                     /locus_tag="AC16BC_11065"
     CDS             complement(6484..6966)
                     /locus_tag="AC16BC_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242201.1"
                     /translation="MTTALPPSDSRDTPTHPLVERLAESVIHEFHQELQDEKIRRRSM
                     ARRHVWGTQPLWVFGVGLMLASGLLLRGDDRWVVLVIGALMLAIEVGLWRPIESKRGL
                     LEGVLSAAVAVTAIARLAESVNNTLTVHHIYLVLALVGAVFVLVESVKRGVAPADPDR
                     "
     gene            complement(7052..7599)
                     /locus_tag="AC16BC_11070"
                     /pseudo
     CDS             complement(7052..7599)
                     /locus_tag="AC16BC_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7796..8689
                     /gene="truA"
                     /locus_tag="AC16BC_11075"
     CDS             7796..8689
                     /gene="truA"
                     /locus_tag="AC16BC_11075"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3242202.1"
                     /translation="MTYRKGDPRVTPRRERPPRRPHAARPIRPVATQPTSTAGGSHFK
                     LTIEYEGSRYRGWQEQSNAMTVQEVIKNALNEILGNEYQMAGAGRTDAGVHALAQVVS
                     LHSPRAIAPAVLLDELNRRLTKDVNVLKVEPAAPDFHARHSAVARYYVYQISLRRTAF
                     AKNYCWWVKQALDLGAIDRSLPAFLGLHDYKAFADARMGDGSTKVQVDDLRVARHGDL
                     LLIRIGASHFLWKMVRRIVGVLVKIGVKEVPAGLSLSELGRRYAKQIPTWTAPAAGLF
                     LERVCYPGDPPPGEPVPLIAL"
     gene            8841..>9761
                     /gene="hemE"
                     /locus_tag="AC16BC_11080"
     CDS             8841..>9761
                     /gene="hemE"
                     /locus_tag="AC16BC_11080"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR3242203.1"
                     /translation="MSPQALKNDRVLRALRREPVDVTPVWIMRQAGRYMPEYRAIRAE
                     ADFLTLCKTPELATRATLLPIDMLGVDAAIIFSDILIPVQAMGMELVFSESKGPQLPS
                     PIRDRSDIDRLQDFDPRTHTRFLPDSIRQTVKALDGRVPLFGFAGAPYTLATYMIEGE
                     TSKNFYGIKRLAYQSPDLLHRLLERLTRMVGDYLIAQVEAGAAAVQIFDTWAGALSPG
                     DYAQFCAPYTARIVEAVKKTGAPVLLYVNGCAALLEAMANTGVDAISVDWRIDLAEAK
                     RRVGSRVALQGNLDPCVLYAQPDVIRRHARR"
BASE COUNT         1625 a         3122 c         3386 g         1628 t
ORIGIN      
        1 ccccagttcc agcgcgcgct ccagattgat cccagctacc atcaggcccg gcgcaacctc
       61 gcgctggcct accaacggac gtttcgctgg gccgatgcct tggcggaata tgagacgttg
      121 ggtcgtctcg ccgacgggag cgacgacgac cgcctgctca tcggagcctt ggccttgcaa
      181 gcgggtgatc tcgacaaggc cgccgcgaac ctgcatgcgg tgatcaatcg tcaccccgac
      241 tcctacccgg cgtggttcaa cctgggcctc gcggaggtgc gacgggggcg cgccacagag
      301 gccgagcagg cgttccaaca ggccgccgcc ttgaacccga acgatcccga tgtgtattac
      361 cagcggggcg tgctcgcgtc gcgcgacggc cgatcgagcg acgccgcgca gttgttcgag
      421 gcggcgctgg cgcgacgccc cgagtacttt ctggcccact acgaattggc ccgacttgag
      481 gacggccgcg ggcggcgcga cgcagccgcg gagcactacc aacggttcgt ggagaccgtt
      541 ccgagggatc cggcatggag cgacgcgcga gccgaggccg acgcctggct cgcggcgcgc
      601 ggtcgttcag gccatggggc ggtcggccgt tagctgattc gcccgaacaa ccgcggcgtt
      661 gcgggtcagg tgtttggagg tgattcgccg taacgcggtg cgggcaaacc gatcgcggta
      721 ctgaccgtgc gatcgcgggg tggcggcatc atccagcatc aaccacgggc cgagttcacc
      781 ggcctggccc cggccggggg cttccggctg ggcgctcgat tccacggggg cctcgttgta
      841 cggacaggct tcctggcaaa gatcgcatcc aaagatccag tcgcccacca acggacgaag
      901 cggcgcggga atctcgttct tgttttcgat cgtcagatac gcgatgcagc ggcccgcgtc
      961 gagctgaaac ggcgcggtca gcgcgcccgt ggggcacgcc tccatgcagc gcgtgcagct
     1021 cccgcattga ttgaccgcgg ggtcgtcaac cggcagctcg atgtcggtga tgagttcgcc
     1081 gagcagaatc cacgatccgt ggtcgggtgt gatgagcaag gtgtgtttcc cgataaagcc
     1141 gagcccggcg tgttgcgcgt aggcccgctc caacaccggc gcgtggtcca cggcgcaacg
     1201 gatcgcggcg tcgggacggt gcgcgagaat aaaggccttg agccgctcca ggcgcggctc
     1261 gatgacgtcg tggtaatccg gcccccacgc gtagcgggcg atcctgcccg caagcgggtc
     1321 cgggggcggc ggatggtcgc ccgcgtaata cgcccagccg agcgcgatca cgctcttggc
     1381 gccgggcagc acgcgagccg ggtcttcccg gcgttccggt tcctgcgcca gataggccat
     1441 gccggcgtgg cgtccgtcct ccagatacgt ccggaacgcg cgttggtgct ccgggagcgg
     1501 agcggccggg gcgatgccga ccgcggcgaa gcccagtgcc agggcttcgc gcttgatcag
     1561 ctcagcgagc gaggtcgaca ccggcggcgt cggccgcgag cggcggccgg tatccgtcgc
     1621 gctgcgcggc gtggtcgagc gggacggtga ggagatcttc cagcgacggg tcgatctcgc
     1681 gtccgagttc atgagcgtgc gcggttttca ggtacccatt gcattcggtg cagatcgcga
     1741 cgtgatagcc gggttcgacg tcctcgatcg aacacgccaa atgttgatcg accgcgttcc
     1801 cgcagaacgg acagccgggg cgcgtgaaga tccacgaggt cgggcagacg ctgcaataca
     1861 gccgccggtc gcgttcgcgt tcctcggcca tgccgggcgg aaagccgcac aacgggcaca
     1921 gcgggcggtt ccacgcccgg tcggtgaaca cgtcggtcaa cgcggggagg agcgcggcga
     1981 gcgacgggcg cagcgcgagg cggaccgcgt acgccacgaa ctcggccggg gcgttcgccg
     2041 ggggcggctc gtggccgccg agcagccgcg cgagccagcg ggcccggtcg gggcccgcgc
     2101 cggacgccgc gtccagcgcg tccaccacgg gcgccagggc gggcgtgcgc gcgcgcacca
     2161 ccgcgagcaa acgggtgaag gtctgtgcca cgtgcgtgag gtccgcggac ccgaagcgcg
     2221 cgggatcgag cagcgggagt ccgcgcgcca gtcgcagcgg gcgctcgtcg gtcggggcca
     2281 acaccggggt cagatcgtcg ggcgtcgcct cgtcccatat ttcgcacacg cggcgataga
     2341 actgcagggg ttcgcgcagg taaggacgct cgcgcatcaa ccgtttgatc agcatcgcgg
     2401 acgccatggc gctcgatcgt accggcccgc acccgggccg gtcaaggcct cgagccttgg
     2461 ttgaccgtgt tcagcgcttg ctcatataat cgacgtcgtg cccacctcat gccccccgtc
     2521 cggagaatct cgtgactgaa cgcgcattgg ttccgcaacg cttctttgaa acccgcttcg
     2581 ggctgacgtc tcgggacttg gagaccgtcg tggcccgcgc catggaacgg cgggtcgaat
     2641 acgcggacct gtattttgag tacgaagtgt cggaggccgt gtcgctcgaa gaggggctgg
     2701 tgaaaaccgc ctcgcgcgac atcagccagg gcgtcggcgt gcgcgcggtg gccggggatc
     2761 gcaccgggta cgcgtactcc aacgacatca gcgttccgca gatcgatctg gcggcgcaaa
     2821 ccgcgcggtc catcgccgac gcggggaaat cgaccagcgt gttggtgccg ggcgggggac
     2881 cgttgatccg ggatctgtac cccgtggatc agtccgtgct ggacgcgccg ttgtccgcgc
     2941 gcatcgacct gctgcaggac atcgaccgcc tcgcccgcgc gtacgaccca cggatccgca
     3001 acgtgatggc cggcctgtcc tgcgaatcga agctcgtcct ggtcgtgacg tcggacggcg
     3061 ccattgtcgg ggatgtccgg ccgctcgcgc ggctgaacgt gacgtgtatc gcccaggacg
     3121 gcagcaaccg gcagatcggg acgtacggcg ggggcggacg catcgggttc gacttctttt
     3181 tcgatcagga ccgctggcgc acgttcaccc gagaggcggc ccgccgcgcg atcgggaacc
     3241 tctcggcccg gcccgcgccg gccggcacca tgaccgtggt gttggggccg gggtggcccg
     3301 gcatcctgtt gcacgaagcg gtcggccacg ggttggaggc ggacttcaat cgcaagggcg
     3361 tgtccgcgtt ttccaaccgc ctggggcagc gcgtggcgtc cgaactctgc accatcgtgg
     3421 atgacggcac gatcgcggga cggcgggggt cgctcaacgt cgacgacgaa gggtcgccca
     3481 gcggccggac ggtgttgatc gagaacggga tcttgcgcgg ctatttgcag gataagacca
     3541 acgcccgctt gatgaagatg ccggtgaccg ggaacggtcg ccgggaaagt ttcgcccacg
     3601 cgcccatgcc gcggatgacg aataccttca tgctcgccgg gtcgtccacg ccggaggaga
     3661 ttctggcctc ggtcgatcac ggactctatg ccgtgtcgtt cggcggcggc caggtggaca
     3721 tcacgagcgg aaagttcgtg ttttcggcca gcgaggccta cctgatcgag aaggggcgcg
     3781 tcacgcatcc ggtcaaaggc gcgacgctga tcggcaacgg tcccgaggtg ttgaaacgcg
     3841 tgtcgatggt gggcaacgat ttatcgctgg acgcgggcgt gggcacgtgc ggcaaagagg
     3901 ggcagtccgt gccggtcggg gtcggcctgc ccacgatcag gatcgatggg ctgaccgtcg
     3961 ggggcacgca gggctgagat gccgatcgaa cgggatctcg ccgaggagtt gctcgccaaa
     4021 gcccgccgac tgggggcgac cgaggccgac gttatggccg tcgagggcac ggcgttttcc
     4081 acccgcgtgc ggctcggcga ggttgataag ctcgaacaag cgagggaacg tcgactggga
     4141 ctgcgcgtga tcgtggatca gcggaccgcg gtcagttcca cggcggacct gtctcccgct
     4201 tcgctcgatg acctggtgtc ggagacgtac gcgcgcgccg tgcgcatggc gcctgatccg
     4261 cacgcgggtc ttcccgccaa ggggcagggt atcgtgtcgg tcccggatct cgacctgtgg
     4321 gacggcgcct acggcgatcc tcccgcggac gagaaaatcg aactggcgcg cgccgccgaa
     4381 gccgcggcgc tcggctttga tccgcggatc agcaactccg agggcgggga gttcgattac
     4441 ggtcggtcgg agatcgtgta cgcggacagc aacggggtgt acggccggta tcgaagctcg
     4501 tcgttctcgt tgtcggtggt ggcgattgcg gcgcgcgagg gtgcaatgca gcgcgactat
     4561 tggtattccg cccggcgcaa attccggttg ctcgatccgc ccgaggccat cggcaaggtc
     4621 gcggcgcagc gcgcgctcgc ccgcttgggc gcgcgcaagg tcaagacgca agaggcgccc
     4681 gtggtcttcg acccgcagat gtcggcgcga ctggtcagcg cgctgaccac cgcggtttcg
     4741 ggctacgcgc tgtacaaggg cgcgtcgttt ctgggcgggg cgctgggtca gaccgtggcc
     4801 tccccgctgg tgaccatcgt ggacgacggc accctgccca cggggctggg gtccaagccg
     4861 ttcgacggcg aggggctgcc gacccgccgg accgtggtgg tcgaccaggg catcctgagc
     4921 agctatctgc tggacacgta ctccgcgaaa aaactcggcc ttgccgcgac cggcaacgcg
     4981 gcccgcggcg tgggggatac tccgagcgtg gctccgacca actgttacct ggccgccggg
     5041 gcctcatccc cgcaagatat catcgcctcg gtccgcaacg ggctctacgt caccgaactg
     5101 atcggattcg gcgtgaacac cgtgaacggc gactattcgc agggcgcgtc gggcctctgg
     5161 attgaaaacg gcgaggtcac gttcccggtg gaagagatca ccattgccgg caatctcaaa
     5221 gacatgttgc gtcagatcga aatggtcggc aacgatttgg agtttcgatc tcgagtgtgc
     5281 gcgcccacgg tcaaaatcgc ctccatgacc atcgccggca catagccggt ccatgaaggg
     5341 ccatctgcgg cgttgccgct gcgcatccgg tgctcacgta cttacctagt acgctccgct
     5401 ccggtgctcg cggcgccttg catctggccc attcctgaac cggctcgccc gtcgcttcac
     5461 tcgggcggat taaacaaacg tcttctaggg caggggctag ggccagcgga tcagcgccgt
     5521 gccccacgta atcccgccgc caaaggccgc cagcaatacc cactctccgg atcgaatgcg
     5581 gccctcggcc actgcgtggt gcagggcgat ggggagcgcg gaggaggagg tgttgccgta
     5641 gcggctgatt gtgaccggga ctttcgcgtc gggcaattgg aggcggcgtg tgacctgttg
     5701 caggatgcgc aggttggctt ggtgaaagat gaagtggtcg atctggtcca gcgggagatc
     5761 gaagcgcgca gcttggtcgc gcaccgcgtg ttcgatggtg cgcaccgcgg tccggaacac
     5821 ggcggatccg tccagatgca tggcgtgacg gttggcggcc agactcgcct cggacaatgg
     5881 ctcgcggctg ccaccggccg gaagatgaat caggggccag cgcgtgccgt ccgcgtgcag
     5941 cgtcacgtcg agcacgccgc gatcctccgg tccctcggac aacaccacgg cgccggcccc
     6001 gtcgccgaat aaaatcgcgc tggtggggtc gcgctggtcg atgaagcgcg acttgacctc
     6061 ggtggctgca agcaaaacgg tccgggcttg tccggtgcgg agaaaggcct gtgcggtgga
     6121 aagcccgtac agaaaccccg tgcacgaggc cgccaggtcg aacgcgggac agccggcggc
     6181 tttcaattcg cgctggagca ggcacgcggt cgacgcaaag cccatgtcgc ctgacgtggt
     6241 ggacaccagc aacaagtcca cgtccgaagg caggcgttgc gcggcattca acgcggcgcg
     6301 ggccgcggtg gcggcgagat cggaagacgc ttcgtgttcg aggacccagc ggcgcgagtc
     6361 gatgccggtt cggcgcagga tcgcgcctgc gggcagggcc agaccggttt cgagttcttc
     6421 gttcgtgacg atccggctcg gaagcccgat cccggtgccg gtgatgaccg cgcccatcag
     6481 gccctaccga tcagggtccg cgggcgctac cccgcgcttg acgctctcca ccagcacgaa
     6541 gaccgccccg accaatgcca gcacgaggta aatgtgatgt acggtcaggg tgttgttgac
     6601 cgactcggcc agccgcgcga tcgcggtgac ggccacggcc gcgctcaaca cgccttccag
     6661 caggccgcgt ttggactcga tcggccgcca gagacccacc tcgatggcga gcatcagcgc
     6721 gccgatgacg agcacgaccc accgatcgtc tccgcgcagc agcaggcccg acgcgagcat
     6781 caatccgacg ccgaacaccc acaacggttg ggtgccccag acgtgtcttc tagccatgga
     6841 gcgtctcctg attttttcgt cctgcaactc ttggtgaaat tcatggatga ccgactccgc
     6901 caggcgttca acgagcggat gcgtcggcgt gtcgcgtgaa tcggatggcg ggagcgcagt
     6961 ggtcatggta ggtccgcgcg ccactctagc aaatcaccgt ccggcgaaca agccggacgg
     7021 gtcatgcgcc acgcgggccg gagcaagggg atcagagccg gagggttttg gccgccaacg
     7081 cggttccttc cacgcgcgcc aggatcttgg cgaacgcggt gtcgcgtgac gtggaggtgt
     7141 cgtcgccgaa cggcgagaac ccgcagtcgt ccgtggtgcc cagttggctc agcggaatat
     7201 attcggcggc ctcgagcacc cgatcccgga cctcttccgg tgtctccact tcgccgcctg
     7261 tacgtagccg tcggcgtaag tcgtgtaccg gaacgggccc gcggtgagcc ggggcagttg
     7321 gcgggtatga ccgtccgcaa agggaatggt cacgccgtcc gaggcgaggc tcttcatccc
     7381 tgatacggga taggtggcaa aactgggctt ggtctgctct ccatccgtga tgaccggcga
     7441 ccccgtggcc tcgaaccgcg agatggtgtc gcgaagcgcc gcgtcgtaga gggcgtctag
     7501 ctccttctgt ggcagacgat cggccgcgaa cgcttggact ccgtcgatca gcgcctgtgg
     7561 cctgggaata ctaccaatcg gctcagtcgg tatgggcatc agacagtgcg cctttagaag
     7621 agtgcgcgac gttgcacgag agaagcctca gcgtacacag caacgtatcg gtgtgctttt
     7681 atcacaccag agccggattt ctccagcggg aaatgatttg ctgattcgtt gactttcccg
     7741 cgccatcgcc cgtaggctgc gggccattgc atgaagaccc gctgagtcaa tacacatgac
     7801 ctaccgcaaa ggcgacccac gcgtgacacc tcgtcgcgag cgcccgcccc gccggcccca
     7861 cgccgcacgg ccaatcaggc cagtcgcgac ccagccgacc tcgaccgccg gggggagtca
     7921 cttcaaactc acgatcgagt acgaggggtc tcggtaccgc gggtggcagg aacagagcaa
     7981 cgcgatgacg gtgcaagaag tcatcaagaa cgcgctcaat gaaatcctgg ggaacgagta
     8041 ccagatggcg ggggcgggac ggaccgacgc gggcgtgcac gcgctcgcgc aggtggtgtc
     8101 gctccactcg ccgcgcgcga tcgcccccgc ggtgttgctg gatgaactca accggcgcct
     8161 caccaaagac gtgaacgtgc tcaaggtgga gcccgcggcg ccggattttc acgcccggca
     8221 ttcggccgtc gcacgctact acgtgtacca gatcagcctg cgacgcaccg cgttcgcgaa
     8281 gaattattgt tggtgggtga agcaggccct cgacttgggc gccattgatc gttcgttgcc
     8341 ggcctttctc ggcctgcacg actacaaggc cttcgccgat gcgcggatgg gcgacggctc
     8401 gaccaaagtc caggtggacg acctgcgggt cgcgcgtcac ggcgacctgc tgttgatccg
     8461 gatcggcgcg tcccattttc tctggaaaat ggtgcggcgc atcgtgggcg tgttggtcaa
     8521 gatcggcgtg aaggaggtgc cggccggact gtcgctctca gagttgggac gtcggtacgc
     8581 caaacagatc cccacctgga ccgcgccggc ggccggtttg ttcttggagc gcgtatgtta
     8641 ccccggcgat cccccgccgg gcgagccggt gccgctgatc gcgctgtaaa cacggcggtt
     8701 accaaggatc aacccccccc catcccccct ttgacaaagg ggggcgaggg gggattttct
     8761 gataggcgtt ctttcctttc ctcaagcgcg tagcgcgcac cgcttgacag tgcgcggctg
     8821 cgctcgatac gatgcctgcc gtgtcgcctc aagcattgaa gaacgaccgc gtcctgcgcg
     8881 cattgcggcg cgaaccagtg gatgtgaccc cggtgtggat catgcgccag gccggtcggt
     8941 acatgccgga gtatcgcgcg atccgcgccg aggcggattt tctcaccctc tgtaagacgc
     9001 cggagctggc gacccgcgcc acgttgctgc cgatcgacat gctgggcgtg gacgcggcca
     9061 tcattttctc ggacattctg attccggtgc aggccatggg catggaactc gtgttctccg
     9121 agtccaaggg cccgcagctt cccagtccga tccgcgaccg atccgatatc gaccgccttc
     9181 aggacttcga cccaagaacc cacacgcggt ttctcccgga ctcgattcgt caaaccgtga
     9241 aagccctgga cggacgggtg ccgttgttcg ggttcgcggg cgcgccatac acattggcca
     9301 cgtacatgat cgagggcgag acctcgaaga atttttacgg gatcaagcgc ctggcgtatc
     9361 agtcgcccga tctcctgcat cgcttgctgg aacgattgac ccgcatggtg ggcgattatc
     9421 tgatcgcgca agtcgaggcc ggcgcggccg cggtgcagat cttcgacacg tgggccggcg
     9481 ccttgtcgcc gggcgattac gcgcagttct gcgcgccgta cacggcccgt atcgtcgagg
     9541 ccgtgaagaa gacgggagcg cccgtgctgt tgtacgtgaa cggctgcgcg gccttgttgg
     9601 aggcgatggc gaataccggc gtggacgcga tcagcgtgga ttggcggatc gatctcgcgg
     9661 aggccaagcg ccgcgtgggc tcgcgggtcg cgctgcaagg caacctggac ccgtgcgtgc
     9721 tctacgcgca gccggacgtg atccgtcgcc acgcgcggcg g
//
LOCUS       DBNGTT010000483         1887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_499160, whole genome shotgun sequence.
ACCESSION   DBNGTT010000483 DBNGTT010000000
VERSION     DBNGTT010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_499160"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="AC16BC_11085"
     CDS             <1..604
                     /locus_tag="AC16BC_11085"
                     /inference="COORDINATES: protein motif:HMM:NF025871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIR3242204.1"
                     /translation="DFPVFLARVHGKNPPMPVRRALAENLYEEETGGLSRGRAHPEMF
                     LYLMEGLKFTARDFDGIQLLPESAAYRRWLDQATTTGSWIEGAAVATIWVEGSVHERA
                     VFDDTVREEPDIERKIAEHPLVKYHGVPPRHLELARAHGAIEGLHRRDAWKIVLDYAT
                     TPETQQRVLHAMERSLELWLAYRDGVAKACGLTRQERVCP"
     gene            920..1837
                     /locus_tag="AC16BC_11090"
     CDS             920..1837
                     /locus_tag="AC16BC_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3242205.1"
                     /translation="MLTPTAVGLLCAVVTCVTWGVADYFAARISRQVGEIAALLRIQL
                     VGAPVLVLAWIALRPPWPATADLLWIAAAAACFLVGYVSFFYGLRVGAISLVSPISSA
                     GAVVPAFVGIALLGEAASGVRMGGMAVALLGVMVLTADPAAIGSLAREGRRSGIKAGI
                     VTLVAWGSGTALLLPAIRSVGAFAPIAILRLEILTLLAAWWLVQRATADTASPGGALP
                     PDVPFAGGWAVVVAASLLDLTAFFSYGVALRDAPAAVAAPIASGYPLVTILIARHRLH
                     ERLSPREWLGVGLTLAGAAVLGASCCDQP"
BASE COUNT          269 a          640 c          626 g          352 t
ORIGIN      
        1 cgacttcccc gtgttcctgg cgcgggtcca cggcaagaat cctccgatgc cggttcggcg
       61 cgcgctggcg gaaaacctct atgaagagga aaccggcggc ctctcgcgag gccgcgccca
      121 cccggagatg ttcctctatc tcatggaggg gctgaaattc accgcgcgcg acttcgacgg
      181 gatccagttg ctgccggaaa gcgcggcgta tcggcgatgg ctcgaccagg ccaccacgac
      241 cgggtcatgg atcgaaggcg ccgccgtggc cacgatctgg gtggaaggca gcgtgcatga
      301 gcgcgcggtg ttcgacgaca cggtccgtga agaacccgac atcgagcgca agatcgcgga
      361 acatccgctg gtcaaatacc acggcgtccc gccccgccac ctggaattgg cccgcgcgca
      421 cggcgccatc gagggtctgc accgtcggga cgcgtggaaa atcgtgctgg actacgcgac
      481 cacccccgaa acgcaacagc gcgtgctgca cgccatggag cggtcgctgg agttgtggtt
      541 ggcgtatcgg gatggcgtgg ccaaggcctg cggcctcacg cggcaggaaa gagtctgtcc
      601 atgagcggtc atctgcggcg ttgtcacgtc ggcttcgcat cctcacgtac gacccagtac
      661 gctgcggtgc gaagcctcgt tccgccttgc atctgacgcg ctcctgaact gaacccgctc
      721 cgctaatgcg gcggagcggg ttcagttcgc aagcattaca agtgtcatta gccttcgttc
      781 ccgtggaggc gggaacccaa caatccaaca tcttctcccc ccctttggaa aagtggggca
      841 cggggggatt ggccttccgc tccacaacgg gtgtcgtcca acttattgac cgcataatag
      901 ccacacgtca acagaccacg tgcttacacc tactgctgtc ggtcttctct gcgccgtcgt
      961 gacgtgcgtg acgtggggcg tcgcggatta ttttgcggcg cgcatctcgc gtcaggtcgg
     1021 cgagatcgcc gcgttgttgc ggatccaact cgtgggcgcg ccggtgttgg ttctcgcgtg
     1081 gatcgcgttg cgcccgccgt ggcccgcgac cgccgatctc ctgtggatcg ccgcggcggc
     1141 cgcgtgtttt ctcgtcggct acgtcagctt tttctacgga ctccgcgtgg gcgcgatctc
     1201 gttggtcagc cccatcagca gcgccggggc cgtggtgccc gcctttgtcg ggatcgcgct
     1261 gctgggagaa gccgcgtccg gcgttcgaat gggcgggatg gccgtggcgc tcctcggcgt
     1321 catggtgttg accgccgacc ccgcggccat cggatcgctg gcccgcgaag ggcggcgcag
     1381 cggcatcaag gccgggatcg tcacgctcgt ggcctgggga tcgggaaccg ccctgctctt
     1441 gcccgcgatt cgaagcgtcg gggccttcgc gcctatcgcc attcttcgct tggaaatcct
     1501 gaccctgctc gccgcgtggt ggctcgtaca acgcgccacc gccgacacgg cgtcccccgg
     1561 cggcgcgctc ccgcccgacg tcccgttcgc gggcggctgg gcagtggtcg tcgcggcgtc
     1621 gctgctcgac ctcaccgcgt ttttctccta cggcgtggcg ttgcgggacg cgccggccgc
     1681 ggtggccgcg cccattgcgt ccggttaccc cttggtgacg attctgatcg cgcggcaccg
     1741 gctccacgag cggctcagcc cccgcgagtg gctaggcgtc ggcctgaccc tcgccggcgc
     1801 cgccgtgctc ggcgcaagct gctgcgacca gccttgagcc cccgggttta agcgtgtccg
     1861 gttactccgg acacgagcct gttctgg
//
LOCUS       DBNGTT010000484         9970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2925924, whole genome shotgun sequence.
ACCESSION   DBNGTT010000484 DBNGTT010000000
VERSION     DBNGTT010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2925924"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="AC16BC_11095"
     CDS             <1..706
                     /locus_tag="AC16BC_11095"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR3242206.1"
                     /translation="AATFACFLTRAYDFIRMGAISGANIKLMGSHAGVSIGEDGASQM
                     GLEDLAMMAALPGVVVLYPSDAYAAYRLVEAAASHRGLVYVRTGRPKCPTLYGPDERF
                     PIGGSKVLRRSAQDRLTIVGAGITVFEALKAHDQLKAAGVSVRVIDLYSIVPADRVTL
                     LDAARATGGQILTVEDHSAHGGVGDAVLDAVAAEGIRVHKLAVREVPHSGKPDELVDR
                     YGISARAIVDAAKRIL"
     gene            complement(772..1392)
                     /locus_tag="AC16BC_11100"
     CDS             complement(772..1392)
                     /locus_tag="AC16BC_11100"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3242207.1"
                     /translation="MNGLRRAVVVGVIAGVCSVGLTANAWSQPPGHPPMGGGMGMGMG
                     MGAGKGPGAGHPGMMFSPSFLKDQLKLSDEQIDKFKKLRNDYERETIRRGADIKIAEM
                     DLWDLFEKKDLTADQLEKKVREIEAKKTDLRVFRFKQVFAIKSILTPEQFEKFRSLGM
                     SMFGGGGGYGRGGGMGMSGHPGMGMGYGDKHRGGYGYGYDQGSDEE"
     gene            complement(1439..1999)
                     /gene="cobO"
                     /locus_tag="AC16BC_11105"
     CDS             complement(1439..1999)
                     /gene="cobO"
                     /locus_tag="AC16BC_11105"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3242208.1"
                     /translation="MAGPQTAPPLGGSRRKGLIIVHTGDGKGKTTAALGLALRAVGHG
                     WRVLMVQFIKGSWQYGELEAAKRLAPDLTIQPMGEGFTWDTKDPARDAAKARACWEAG
                     VAAARSGQYQMVIFDEINYAMAYGYLPLQEVLDFLAAKPADVHVVLTGRDAPKELIAV
                     ADTVTEMRAIKHAFEQGVKAQKGIEF"
     gene            complement(2082..2798)
                     /locus_tag="AC16BC_11110"
     CDS             complement(2082..2798)
                     /locus_tag="AC16BC_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR3242209.1"
                     /translation="MTRIATSDQPAVAMRISGIEPQGVEDIRSIRLVPASADRPNFTF
                     IPGQFLHIRTPSGEASYFAIASVPGEPYFDVLVKRAQGASREMFDLPVGTTVSVVGPQ
                     GKGFPLDAYEGFDVLLIGVGTGIAPLRSVAGWVLANRRRFGRLTLLYGVLTPSHWCYR
                     SDFSAWQAEGVHTRVTVTSTEGLAWSGPVGFVQAVLSDMEIDPRRTVACLVGMKDMVR
                     ANTELLTHRGVPSDRILLNF"
     gene            complement(2863..5463)
                     /locus_tag="AC16BC_11115"
     CDS             complement(2863..5463)
                     /locus_tag="AC16BC_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3242210.1"
                     /translation="MTGRGVALAALAVLIASVAGRPAYSASVDPLLLPVLAEQAAPEI
                     LGPAFVGVAGGTSSEEPSISVSVRFRGPIADLRALGIAFNATLGDVATADVSIVQLRA
                     LAAHPAVLTIEAATLLAPNLDASVPATGANLLRTQAGSGWTDTSYTGRGVLIGIIDTG
                     IDLRHEDFLKPDGTTRALAVWDQSSSVGRPPRGFSYGAECTADQINVRDCPMVDRNGH
                     GTHVAGIAAGDGSATGQGQAAYRYIGMAPEADLLVVKLGASTSTRIMDGLSYLKDKAT
                     RLGKPIVVNLSYGAPLGPHDGTLNLDAAVDNFTGPDDSPGAVVVVSAGNSGQTSSGGP
                     LHAVGCFQNGPTPPNCPAGITALPAANPATLSFLVPTGTQTVYLDVWYPGGPPTNRNV
                     LGVKVASPVACATQSAVPGSPPVVASTPCGSILIAASDVYAPNGDRHSLVLLTSSSEI
                     TSGVWTLAITGNSLPPDSVTRFDVWPDAVPRGNAPSFTSLGSAETTVETPASAAEAIA
                     VVPYVTKPTWTTVDGGCGGGTTFNQLAPYASRGPLRPCTTCGTQAEKPDLAAPGLVVF
                     SSLSSRIVAGATDLICRVDLDRRHYALDGSSMAAPHVTGAAALLLQINRNLTARQVKS
                     YLRSNTWLPQPTPATQWGQGRLNIKTAVDALKAAPGGDPAPAPPTGLRVTSVHSHRVA
                     LAWDANPDLDLQKYQVLRRAENEAVAIPLIPFLEPTVTTFEDTGLAANDTAYYYSVQA
                     VDIAGQTISPSTEVRAVPTEGDGSVGLCFIATAAYGSAWHPHVASLRAFRDQRLRPHA
                     LGRAFITLYETVSPPIAGWIAPHPTLRAITRGALTPVVFAVEQPRATAILAGLGLAAL
                     VGLRLRRRLP"
     gene            complement(5460..5831)
                     /gene="secG"
                     /locus_tag="AC16BC_11120"
     CDS             complement(5460..5831)
                     /gene="secG"
                     /locus_tag="AC16BC_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR3242211.1"
                     /translation="MTVLIIIVHVLVCLIMIGVILLQAGKGAEIGAAFGGSSQTIFGS
                     RGATTFLSKLTIGAASLFMLTSLSLSILSNQRSVASSIIDALPPAATTPAPASPNAAG
                     EPAPGEASSSAPSAPTTPATP"
     gene            complement(5828..6658)
                     /gene="tpiA"
                     /locus_tag="AC16BC_11125"
     CDS             complement(5828..6658)
                     /gene="tpiA"
                     /locus_tag="AC16BC_11125"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3242212.1"
                     /translation="MTSAPVSAPLIIANWKMHKTVADARSAVQALRARPDLLAAVDVV
                     LAPPATALDAARQAIAGDRLGLSAQTCHWESAGAYTGEVSAPQLADVGCTYCLVGHSE
                     RRQGFGETNDHVKRKVAALLAAGLTPVLCVGESFAERRAGKTEPVVAEQVSVALQGLS
                     TGHVARVVVAYEPVWAIGSGKTATPAEAVTVHRFIRQLITADHGAAGLRILYGGSVSP
                     ETIGGLLAQPDINGALVGGASLSITTFLALLERAAQRNSGTSPPASHRSPPTPARSFA
                     "
     gene            complement(6658..7857)
                     /locus_tag="AC16BC_11130"
     CDS             complement(6658..7857)
                     /locus_tag="AC16BC_11130"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3242213.1"
                     /translation="MNIHKQTIDDLNLRDQRVFIRADFNVPLDDDLNITDDTRIRSTL
                     PTINHAIDEGAKVILASHLGRPKNQPDPRYSLAPVAKRLQRFLKKDVRFLPDCVGASV
                     EDAVAKMRPGDVVLLENLRFHAGEEANDDGFAKSLARLADIYINDAFGTVHRSHASVT
                     GITKFVRTSAAGFLLKKEIEYLEGAVANPARPFVAVLGGAKVSGKLGVINNLQKKVDK
                     VIIGGGMAFTFFKAFGLEVGNSLVEPNMLDVARQMREAGRTHGLKFYLPVDCVVATGM
                     EPGAETKIVPVQEIPKGWTALDIGPASVRLFTEALANAKTILWNGPMGVFEMDAYSRG
                     TLAVAHAVANAYALTIVGGGDTALAVHRAGESENISFISTGGGAALQLLEGKPLPGLT
                     ALPDRES"
     gene            complement(7861..8871)
                     /gene="gap"
                     /locus_tag="AC16BC_11135"
     CDS             complement(7861..8871)
                     /gene="gap"
                     /locus_tag="AC16BC_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3242214.1"
                     /translation="MSVRVGINGFGRIGRAVLRASLAESDLEIVAINDLTDAKTLAHL
                     LTYDSVHGRLDVPVAARDSAITVGGREIAVLALKDPAELPWKKLGVEIVIESTGHFED
                     RPSASKHLAAGAAKVIISAPAKGPDLTVVLGVNDERYDAATHHIVSNASCTTNCLAPI
                     AKVLHDAFTIKRGLMTTIHSYTNDQRLLDLPHKDLRRARAANLSMIPTSTGAARAVGE
                     VLPSLKGKIDGMAIRVPTPNVSIVDFVAEVERTATERDVNAALRAAADGPLKGILAYN
                     EELTVSIDHNGSSYSSIVDAALTRVIDGGMVKVCAWYDNEWGYSCRVRDLVKFVAHRM
                     RS"
     gene            complement(9000..9076)
                     /locus_tag="AC16BC_11140"
     tRNA            complement(9000..9076)
                     /locus_tag="AC16BC_11140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9040..9042),aa:Arg,seq:tcg)
     gene            complement(9148..9222)
                     /locus_tag="AC16BC_11145"
     tRNA            complement(9148..9222)
                     /locus_tag="AC16BC_11145"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9188..9190),aa:His,seq:gtg)
     gene            complement(9296..>9970)
                     /gene="rlmN"
                     /locus_tag="AC16BC_11150"
     CDS             complement(9296..>9970)
                     /gene="rlmN"
                     /locus_tag="AC16BC_11150"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HIR3242215.1"
                     /translation="RGHEIVDQVLGARAHAGPERQITNLVFMGMGEPLANLPAVCDAI
                     ERLTADWGLGFSPRRITVSTAGLVPQMATLGAFRHQVNLAISLNATTDATRDRVMGTI
                     NRKYPLETLLAACRAYPLAPRRRIFFEYVLLAGVNDSVADARRLVGLLHGIRCKVNLI
                     PFNPWPGAPFQRPSDDAVAAFQAVLLSAHFTVFVRKSKGRQILAACGQLRSAHDQPAS
                     LVPLQA"
BASE COUNT         1657 a         3210 c         3389 g         1714 t
ORIGIN      
        1 tgcggcgacc ttcgcgtgtt ttctcacccg cgcgtacgac tttatccgca tgggcgcgat
       61 cagcggcgcg aacatcaagc tcatgggttc ccatgccggg gtgagtatcg gggaggacgg
      121 cgcgtcccag atggggctcg aagacctggc gatgatggcg gcgctgccgg gcgtggtggt
      181 gctgtatccg tcagacgcgt acgcggcgta ccggttggtc gaggcggccg cttcgcatcg
      241 cgggctcgtg tacgtgcgga ccgggcggcc gaagtgtccg accctctacg ggcccgacga
      301 acggtttccg atcggcgggt ccaaggtgtt gcgacgcagc gcgcaggatc gcctgaccat
      361 cgtgggggcg ggcatcacgg tgtttgaagc gctcaaggcc cacgaccaac tcaaggccgc
      421 gggcgtctcg gtgcgggtga tcgatctgta cagcatcgtg cccgcggacc gcgtcacgct
      481 gttggatgcg gcgcgcgcga cgggcggcca gatcctcacg gttgaggacc actccgcgca
      541 tggcggcgtg ggtgatgccg tgttggacgc ggtggcagca gagggaatcc gcgtgcataa
      601 actcgccgtg cgcgaagtgc cgcacagcgg caagccggac gaattggtcg atcgatacgg
      661 aatcagcgcc cgagccatcg tggacgctgc gaagcgcatc ctgtaaaaat gaacgggcat
      721 cgggacgtcc caggacgccc cgatgcccgt tttactccgt gttgtggcga cttattcctc
      781 gtcgctccct tggtcgtagc cgtatccgta cccgccacgg tgcttgtcgc cgtatcccat
      841 gcccatgccg gggtgtcccg acatgcccat gcctccgccc cggccgtacc ctccgccacc
      901 cccgaacatg ctcatgccga gcgagcggaa cttttcaaac tgttcgggcg tcagaatgga
      961 tttaatcgcg aacacctgct tgaaccggaa cacccggagg tcggttttct tcgcttcaat
     1021 ctcgcggact tttttctcga gctgatccgc ggtgaggtcc tttttctcga ataaatccca
     1081 caggtccatt tccgcaatct tgatatccgc gccgcgccgg atggtctcgc gttcgtagtc
     1141 gttgcgaagc ttcttgaatt tgtcgatctg ctcgtcggag agtttcagtt ggtccttcaa
     1201 gaacgagggg gagaacatca tgccggggtg tccggctccc gggcccttgc cggcacccat
     1261 tcccatgccc attcccatgc cgccgcccat cgggggatgc cccggaggct gtgaccacgc
     1321 gttcgcggta agaccgaccg agcacactcc ggcgatgacg ccgacgacga ctgccctcct
     1381 caacccgttc atgcgctgac tcctttcgtg gatgagagtt cccgcttcct ggtgcgtgtt
     1441 agaactcgat gcccttctgc gctttgacgc cttgttcgaa cgcgtgtttg atcgcgcgca
     1501 tctcggtaac cgtgtcggcg actgcgatca gttccttggg cgcgtcccgg ccggtcagga
     1561 cgacgtggac gtcggccggt ttggcggcga gaaaatcgag gacctcctgc agcggcaggt
     1621 atccataggc catggcgtag ttgatctcat cgaagatcac catttgatat tgaccggatc
     1681 gagcggcggc cacgccggcc tcccagcacg cacgggcttt cgccgcgtcc cgggccgggt
     1741 ccttggtgtc ccaggtgaag ccctcgccca tgggctgaat ggtgaggtcg ggcgccaggc
     1801 gtttggcggc ctccaattcg ccgtactgcc aactgccctt gatgaattgc accatgagaa
     1861 cgcgccaccc gtggcccacc gcccgcagcg ccaatccaag cgccgcggtg gttttgccct
     1921 tgccgtcgcc ggtgtggacg atgatgagcc ctttgcggcg actgccgccg agcggcggcg
     1981 ctgtctgcgg tccggccatc agcgtgttcc cgaacgcagc gagtatatca gagaacccgg
     2041 gtgggtgcta cgcgcccgcc gcggccggca ttcacgcggt gtcagaaatt cagcaggatg
     2101 cggtccgaag ggacaccgcg gtgggtcaga agctcggtgt tggcgcggac catgtccttc
     2161 atccccacca ggcaggcgac ggttctccgt gggtcgatct ccatgtccga caacacggct
     2221 tggacgaacc ccaccggacc gctccaggcc aggccctcgg tcgaggtcac ggtcacgcgc
     2281 gtgtggactc cctccgcctg ccaggcggaa aaatcgctcc gatagcacca atgtgacggc
     2341 gtcaacacgc cgtataacag cgtcagccgg ccgaagcgtc ggcgattcgc gagcacccat
     2401 ccggcgacgg agcgcaacgg cgcgatgccg gtcccgaccc cgatcaacag cacgtcgaac
     2461 ccctcgtacg cgtcaagcgg gaatcctttg ccctgtgggc ccaccacgga caccgtggtg
     2521 ccgaccggga ggtcgaacat ctcccgcgag gcgccctgcg cgcgtttgac caatacatcg
     2581 aaatagggtt cccccggaac cgaggcgatc gcgaagtacg aggcctcgcc tgacggggtt
     2641 cggatgtgca ggaattgtcc ggggataaac gtgaaattcg ggcggtccgc ggaggccggg
     2701 accaaccgaa tggagcggat gtcctcgacg ccttgcggct cgatgccgct gattcgcatg
     2761 gcgaccgccg gctggtcgct ggtcgcgatc cgcgtcacgg gcgggccgtt ggcaggaggc
     2821 tcagcagcac ggcgcggatc ataatgcgtg ggccgcggca aatcaaggta aacgacgtcg
     2881 gagtcgcagt ccgacgagcg cggccaagcc gagacccgcc aggatggccg tcgcgcgcgg
     2941 ttgctccacg gcgaacacca ccggcgtcaa cgccccccgc gtgatggcgc gcagcgtggg
     3001 gtgcggggcg atccatccgg cgatcggagg gctgaccgtc tcgtagagag tgatgaacgc
     3061 gcgtcccagc gcgtgcggtc gaagccgctg gtcgcggaat gcccgtaagg aggcgacgtg
     3121 tggatgccac gcgctcccgt acgccgcggt cgcgataaag cacaggccga cggacccgtc
     3181 gccctccgtg ggcacggccc tgacctcggt ggacggggag atggtctggc ccgcgatgtc
     3241 cacggcctgc accgagtagt aatacgcggt gtcgttggcc gcgagcccgg tgtcttcgaa
     3301 tgtcgtgacc gtgggctcca ggaagggaat gagcggaatc gcgaccgcct cgttttcggc
     3361 ccggcgcaac acttggtact tctgcagatc caggtcagga ttagcgtccc acgccagggc
     3421 gactcgatgg ctgtggaccg aggtgacccg cagtcctgtt ggtggggctg gggcagggtc
     3481 cccgccaggc gctgccttga gcgcgtcaac ggcggtcttg atattcagcc gaccctggcc
     3541 ccactgcgtc gcgggcgtgg gctgggggag ccaggtgttg gaacgcaggt agctcttgac
     3601 ctgtctggcg gtgaggttgc gattgatctg cagcaacagc gcggcggcgc ctgtcacgtg
     3661 cggcgcggcc atgctggatc catccaacgc ataatgacgg cggtcgaggt cgacccgaca
     3721 gatcaggtcc gtggctcccg ccacgatgcg cgatgacaaa ctcgaaaaga cgaccagccc
     3781 cggggcggcg aggtcgggct tctcggcctg cgttccgcag gtcgtgcacg ggcgtaacgg
     3841 ccctcggctc gcgtacggcg ccaattggtt gaaggtggta cccccgccgc accccccatc
     3901 cacggtggtc cacgtcggtt tggtgacgta gggcacgacc gcgatcgcct cggcggcgga
     3961 ggccggcgtt tcaacagtgg tctccgcact gcccagggag gtgaaacttg gggcattccc
     4021 ccggggcacg gcatcgggcc acacgtcgaa ccgggtgacg ctgtccggcg gcagggagtt
     4081 tccggtgatg gcgagcgtcc agaccccgga ggtgatctcg gacgaactcg tcaggagcac
     4141 caacgagtgg cggtcgccgt tcggcgcgta cacgtcggag gcggcaatca ggatcgagcc
     4201 gcacggggtg ctggccacga ccggcggcga gccgggcacg gccgactgtg tggcgcaggc
     4261 cacggggctg gccaccttga cccccagcac gtttcggttc gtgggcggac cacctggata
     4321 ccagacatcg agatagaccg tctgggtacc ggtgggcacg agaaaactca gcgtggccgg
     4381 attcgcggcc ggcagcgcgg tgatgccggc cgggcagttc ggcggcgtcg gtccgttttg
     4441 aaagcagccg accgcgtgga gggggccgcc ggagctggtt tggccgctgt tgcccgcgga
     4501 taccacgacc acggcgccag gactgtcgtc cggacccgtg aagttgtcga cggccgcatc
     4561 caggttgagc gtcccgtcgt gggggcccaa cggcgccccg taactcagat tgaccacgat
     4621 gggtttgccg agccgtgtgg ccttgtcttt gaggtaactc aagccgtcca tgatgcgcgt
     4681 cgaggtgctg gcgccgagtt tcacgacgag caggtccgct tcaggggcca tcccgatgta
     4741 tcggtacgcg gcctggcctt gtcccgtggc ggagccgtcg ccggccgcga tgccggccac
     4801 gtgggtccca tggccgttgc ggtcgaccat tgggcaatcc cggacgttga tctgatcggc
     4861 ggtacactcc gcgccgtacg aaaacccgcg gggcgggcgg cctaccgagc tggattggtc
     4921 ccagaccgcc agcgcgcggg tcgtgccgtc cggtttgaga aagtcctcgt gccggaggtc
     4981 gattccggtg tcgatgatcc cgatcaggac gccgcgaccg gtgtagctgg tgtcggtcca
     5041 tccagatccg gcctgcgtgc gcaggaggtt ggctccggtc gccggcacgc tggcgtcaag
     5101 gttgggcgcg aggagcgtgg cggcctcgat agtgagcacg gcggggtggg ccgccaaggc
     5161 gcgcaactgg acgatcgaca cgtccgcggt cgccacatcg cccagcgtgg cgttgaaggc
     5221 gatcccgagc gcgcgaaggt ccgcgatcgg tccgcgaaat cgcacgctga ccgagatgga
     5281 gggctcttcg gaagacgtcc cgcccgcaac gcccacgaag gccggcccca gaatctccgg
     5341 cgcggcctgt tccgccagaa ccggaagcag gagcggatcg acggaggcgc tgtacgccgg
     5401 gcgcccggcc acgctcgcga tgaggaccgc gagcgcggcc agggcgacgc cgcgacccgt
     5461 catggggtgg cgggcgtggt cggggcgctc ggcgcggacg acgaggcctc tccgggcgcg
     5521 ggttcgccgg cggcgttcgg agacgccggc gccggggtcg tggcggccgg cggcagcgcg
     5581 tcgatgatcg aggaggcgac tgaacgttgg ttcgaaagaa tcgagagact gagcgaggtc
     5641 agcatgaaca gcgacgcggc cccgatcgtc aatttgctca ggaacgtggt ggcgccgcga
     5701 cttccgaaga tggtctggct ggatccgccg aacgcggcgc cgatctccgc gcctttaccg
     5761 gcctgcaaca ggatcacgcc gatcatgatc aggcagacga ggacgtgaac gatgatgatc
     5821 aatacggtca tgcgaaactc cttgcggggg tcgggggtga acgatgtgat gcgggaggcg
     5881 acgtccccga gttccgttgc gccgcccgct caagaagagc caggaacgtg gtgatggaaa
     5941 gactcgcgcc gcccaccagc gctccgttga tgtcgggttg cgccaacagg ccgccgatgg
     6001 tttccggcga cacgctgccg ccgtacaaga tgcgcaggcc ggcggcgccg tggtcggcgg
     6061 tgattaactg gcgaataaac cgatggaccg tgaccgcttc ggcgggggtc gcggttttcc
     6121 ccgagccgat ggcccacacc ggctcgtacg cgaccaccac gcgcgcgacg tggccggtcg
     6181 agagcccctg gagcgcgacc gagacttgtt cggccacaac cggttcggtt ttgcccgccc
     6241 gccgctcggc gaaactttcg ccgacgcaca gcaccggcgt gaggccggcc gccagcagcg
     6301 ccgcgacctt gcgcttcacg tggtcgttgg tctcgccgaa gccctgtcgg cgttcggagt
     6361 ggccgaccag gcagtacgtg cagcccacgt cggccagttg gggagccgaa acctccccgg
     6421 tgtacgcgcc ggctgattcc caatgacagg tttgcgcgct cagcccgagt cggtcgccgg
     6481 cgatcgcttg gcgcgcggcg tccagcgcgg tcgcgggcgg cgccagcacg acatccaccg
     6541 ccgccaagag atccggtcgc gcccgcaggg cttggacggc ggaccgggca tcggccacgg
     6601 tcttgtgcat tttccagttg gcgatgatca ggggcgcgga aaccggggcg gacgtcatca
     6661 ggattcgcgg tccggaagcg cggtgaggcc gggcaggggt ttgccctcga gcaactgcaa
     6721 cgcggctccg cccccggtgg aaatgaatga aatattctcc gattcgcccg cgcggtgcac
     6781 ggccaacgcg gtgtcgccgc cgcccacaat ggtcagggcg tacgcgttcg cgaccgcgtg
     6841 cgccacggcc aacgtgccgc gcgagtacgc gtccatttcg aacacgccca tcgggccgtt
     6901 ccacaagatg gtcttggcgt tggccagcgc ttcggtaaac agacgcaccg acgcgggccc
     6961 gatatcgagc gcggtccagc ccttggggat ttcctggacc gggacgatct tggtttcggc
     7021 gccgggttcc atcccggtgg ccaccacgca atccactggt aaatagaact tcagtccgtg
     7081 ggtacggccc gcttcccgca tctgccgtgc cacgtcgagc atgttcggtt caaccaggga
     7141 gttccccacc tccagaccaa aggctttaaa aaaggtaaag gccataccgc ccccgataat
     7201 aaccttgtcc acctttttct ggaggttgtt gatcacgccg agcttgccgg acaccttggc
     7261 gccgccgagg accgcgacga acggtcgcgc gggattggcc accgcgccct cgaggtattc
     7321 gatttccttc ttcagcaaga agcccgcggc cgaggttctc acgaacttgg tgatgcccgt
     7381 gaccgacgcg tggctccggt gcacggtgcc gaacgcgtcg ttgatataga tatcggccag
     7441 ccgcgccagc gacttggcaa acccgtcgtc gttggcctcc tctccggcgt gaaaccgaag
     7501 attctccagt aagaccacat cgcccggccg catcttggcc accgcgtcct ccacggatgc
     7561 gcccacgcaa tccggcagga accggacgtc cttcttgagg aagcgttgga ggcgcttggc
     7621 gacgggggcg aggctgtagc gcgggtccgg ctggttcttg ggccgcccca ggtgcgaggc
     7681 gagaatgacc ttggcgccct cgtcgatcgc gtggttgatc gtgggcagcg tggaccggat
     7741 ccgcgtgtcg tccgtgatgt tgaggtcgtc atccagcggc acgttgaagt ccgcgcggat
     7801 gaacacgcgt tggtcgcgca agttcaggtc atcgatcgtc tgtttgtgga tgttcatggc
     7861 ttacgaccgc atccggtgcg ccacgaactt gaccaggtcc cgcacccggc aggaataacc
     7921 ccactcgttg tcgtaccagg cgcagacctt gaccataccg ccgtcgatga cgcgggtgag
     7981 cgccgcgtcc acgatcgagg aatatgaact gccgttgtga tcgatggaca cggtcaattc
     8041 ctcgttgtac gcgaggatgc ccttgagcgg gccgtcggcc gcggcccgca gcgccgcgtt
     8101 cacgtcgcgt tcggtcgcgg tccgctcgac ctcggcgaca aagtccacga tggagacgtt
     8161 cggcgtgggc acccggatcg ccatgccgtc gattttgccc ttcagcgagg ggaggacttc
     8221 ccccaccgcg cgcgcggcgc cggtcgaggt gggaatcatc gaaagatttg cggcgcgtgc
     8281 gcgccgcaaa tccttgtgcg gcagatccag gagccgttgg tcgttggtgt atgaatgaat
     8341 ggtggtcatg aggccgcgct tgatcgtgaa ggcgtcgtgc aggaccttcg caatgggagc
     8401 caggcagttg gtggtgcagg acgcgttgga cacgatatgg tgcgtcgcgg catcgtaccg
     8461 ctcgtcattg acccccagca cgaccgtgag atccggaccc ttcgccgggg cggagatgat
     8521 gaccttagcc gcgcccgccg ccaagtgttt ggacgccgag gggcggtcct cgaagtgtcc
     8581 ggtcgattca atgacaatct cgacgccgag tttcttccac ggcaactcgg cgggatcctt
     8641 cagcgccaac accgcgattt cgcgcccccc cacggtgatc gcgctgtctc gggccgcgac
     8701 cgggacgtcc agccgaccgt gcaccgagtc gtaggtgagc aggtgcgcca gcgtcttggc
     8761 atccgtcagg tcgttgatcg cgacgatctc aagatcagat tccgcgagcg acgcgcgcaa
     8821 caccgcccgc ccaatccgac cgaatccatt gattcccact cgaacgctca taccgtctcc
     8881 gtctttatgc ggtaaaaccc gctcatctta ggtctggaaa acggccaaag tcaaacgccg
     8941 gactcgcaag cgggaagggt cgcgcagtag tatgtgccgg ggtgcggata ccgtccgagt
     9001 ggcgcgcctg gagggagttg aacccccgac ctgcggattc gaagtccgac gctctatcca
     9061 gctgagctac aggcgcggaa tccccggcgg ccgctcgata cacgcggcgg gacaaacggg
     9121 gcgtttgatc actgggagcc ggcggcgtgg ggtgagtgag gggtttcgaa cccccaaccc
     9181 ctggagccac agtccagtgc tctacccttg agctacactc accacgtcgg ccgccgagcg
     9241 ttcggttgta tcaccccgtc gcgagacttg tcaacggcgc gaccgtggtc gcgcgctagg
     9301 cctgaagcgg gaccagcgag gcgggctggt cgtgggccga ccgcagttgc ccgcacgcgg
     9361 ccagaatctg acggcctttg ctttttcgga caaacaccgt gaaatgcgcg ctgagcaaca
     9421 cggcctggaa cgccgcgacc gcgtcatccg acgggcgttg aaacggcgcg ccgggccagg
     9481 gattgaacgg aatcagattc accttgcagc ggatcccgtg caggaggccc accaatcgtc
     9541 tcgcgtcggc cacggagtcg ttgacgccgg cgagcaacac gtattcgaag aagatccgcc
     9601 gacgcggcgc caacgggtac gcgcggcagg cggcgagcag cgtctcgagc ggatacttcc
     9661 ggttgatcgt gcccatcacg cggtcgcgcg tcgcatcggt cgtggcgttg agcgagatgg
     9721 ccaggttgac ctggtgccgg aacgctccca gcgtcgccat ctgggggacc agtcccgcgg
     9781 tcgacaccgt gatccgtcgc ggggaaaatc ccaggcccca gtccgcggtc aggcgctcaa
     9841 tggcgtcgca caccgcgggc aggttggcca acggttcacc catgcccatg aatacgaggt
     9901 tggtgatctg ccgctccggg cctgcgtgcg cccgcgcgcc caagacctga tccacgattt
     9961 cgtggccgcg
//
LOCUS       DBNGTT010000485         1627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_756058, whole genome shotgun sequence.
ACCESSION   DBNGTT010000485 DBNGTT010000000
VERSION     DBNGTT010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_756058"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1437)
                     /locus_tag="AC16BC_11155"
     CDS             complement(13..1437)
                     /locus_tag="AC16BC_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HIR3242216.1"
                     /translation="MLDRIVGVETEYGVLAKTDDRFRAPERIAHRLRDFIFHEQRLGL
                     IDLHHRDYDEPIGNGGFLLNAGRLYVDMGHLEYASPECATLADAVAYERAGDVIVQRA
                     LEAAGLRDQASVIKNNIDHETDATFGSHENYLVRRSFPFTREGLDQLVPFLVTRQIFA
                     GAGRVGAARAASGLVRIGEDAERPDVDFQIAQRSDHIVNEFFQWVQGNRAIINTRDEP
                     LADPNRYRRIHLLLGDSNLCEVAIALKIGTTSLVLQLIEDDACPGGLSPADAVGSLHA
                     ISRDPSRKWIVELADGRTISAIEIQERYAEAARDRYYGRDEEMDWVLDEWERVLTDLR
                     GDHERLVGRVDWASKYWLLNNFREAEQLDWRDPWIKSLDLAYHNLETSKGLYFALADE
                     GRVRRYITDDAAALAADNPPRNTRAFGRGEAIRRMMAGDARYVITWAGLLVEGKPPLR
                     MPDPFKTYTDAVADQFGAIVPPGD"
BASE COUNT          265 a          568 c          517 g          277 t
ORIGIN      
        1 ttcagacggt tgtcaatctc cagggggcac gattgcgcca aactggtctg ccaccgcatc
       61 cgtatacgtc ttgaacggat ccggcatccg caacggcggc ttcccctcga ccaacagccc
      121 cgcccacgtg atcacgtacc gcgcgtcgcc ggccatcatg cggcgaatgg cctcgccacg
      181 cccgaacgcg cgcgtgttgc gcggcggatt gtccgcggcc agcgcggccg cgtcgtccgt
      241 gatgtagcga cggacccgcc cctcgtcggc caatgcgaag tagagcccct tggacgtttc
      301 gaggttgtgg taggccaggt ccaagctctt gatccacggg tcgcgccaat cgagctgctc
      361 ggcctctcga aaattgttca gcaaccagta tttggaggcc cagtccacgc ggcccacgag
      421 ccgctcgtga tcgccgcgca aatcggtcag cacgcgctcc cattcatcca ggacccagtc
      481 catctcctcg tctcgcccgt agtaccggtc ccgcgcggcc tcggcatacc gctcctgaat
      541 ctcgatcgcc gagatcgtcc gcccgtcggc gagttccacg atccacttcc gcgaggggtc
      601 tcgggaaatc gcgtgcaggg accccaccgc gtcggccggc gacaggcccc cgggacacgc
      661 gtcgtcttcg atcaattgca ggaccagcga cgtggtcccg attttgagcg cgatggccac
      721 ctcgcacagg ttcgaatcgc ccagcagcag gtgaatccgg cggtaccggt tgggatcggc
      781 gagcggctcg tcgcgggtgt tgatgatcgc ccggttgccc tgcacccact ggaaaaactc
      841 gttgacgata tgatcggacc gctgcgcgat ctgaaagtcc acgtccggcc gctccgcgtc
      901 ctcgccgatc cgcaccaggc ccgaggccgc gcgcgcggcc cccacccggc cggcgccggc
      961 gaagatctgg cgcgtgacca ggaacgggac caattggtcg agcccttcgc gggtgaacgg
     1021 gaacgagcgc cggaccagat agttctcatg cgacccgaac gtggcgtcgg tctcatggtc
     1081 gatgttgttc ttgatgaccg acgcctgatc cctcaggccc gcggcttcca gcgcgcgctg
     1141 cacgatcacg tcgccggcgc gttcgtacgc caccgcgtcc gccagcgtgg cgcactcggg
     1201 actcgcgtac tccaggtgcc ccatgtccac gtagagccgg cccgcgttca gcaggaaccc
     1261 gccgttgccg atgggttcgt cgtagtcgcg atggtggagg tcgatcagcc cgaggcgctg
     1321 ctcgtggaaa atgaagtcgc gcaggcgatg ggcgatgcgc tccggcgcgc gaaaccggtc
     1381 gtccgtcttc gccaggaccc cgtattcggt ttcaacgccg acgatgcggt cgagcatgga
     1441 acgccggtct aacgaaccgc ctcgcccagc cagggcacca ggatctgcga cacttcggcg
     1501 gggatgagcg cgcggtactt ggagctaccg ggcacggtgc gatccagcac cgcgacttcg
     1561 atccggcgat ccttgcaaca tgtcttgaga tggtcggtca gccgcgcgtc gtccggagcc
     1621 ggcgcgg
//
LOCUS       DBNGTT010000486         4009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_245432, whole genome shotgun sequence.
ACCESSION   DBNGTT010000486 DBNGTT010000000
VERSION     DBNGTT010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_245432"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /locus_tag="AC16BC_11160"
     CDS             <1..1622
                     /locus_tag="AC16BC_11160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3242217.1"
                     /translation="DEAVFAEATREMVATGDWITPQYNGADRFDKPVLFYWLMASAYL
                     VFGVGEFAARFWSAAFGVGLVGLLAVFARRRLGFRVGLLSGLVAATSLEVVVLAHAAI
                     TDMVLIFFLSAGLLALFAAVQATEAGGRTESALISSAAFGLAVLTKGPVGIVLPGMIL
                     LVFLALRGGLRDALANLPWGRMALVFALVAAPWYALELHARGWAFIEGFFLKHNVRRF
                     TGVISGHAGPFYYYIPAVAVGFFPWTSVLLPAIRWAWPSGWRGLRGPTDREALGLFVL
                     VWIGVVFAFFSAAGTKLPNYVASLYPPLALLTGMWAERFVARAPGADRWIGAGMAVLV
                     GVALIMAVAFWGVPWWLEWARVRFPKAAYLHQPVDAGWGPAALGAVCVIGAVAIAWTA
                     MRRRPGAMIGAVAATTVTATIVLLTAVFPVAADVIQVPLRDLSMRASREAGPDGGIAV
                     YGLNKPSVVFYAKRLVTVVGESRPEELGALIREDRAWFVITKARWLDRLDLFGPIHVV
                     ESRGGYVLASNRPAALGGAAPGETGGRDAGS"
     gene            1609..2589
                     /locus_tag="AC16BC_11165"
     CDS             1609..2589
                     /locus_tag="AC16BC_11165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3242218.1"
                     /translation="MPDREPKRPALSIVIPLFNEAESLPALAERVFGAVARYGLDAEV
                     LFVDDGSTDGSEGIYRRLCDVHPALRVIRLRRNFGQTAAMAAGFDHARGAVIVVLDGD
                     LQNDPDDIPLLLKKLDEGFDVVSGWRRDRRDHWSRVLPSRLANWLIGRTTGVRLHDYG
                     CSLKAYRAELIQNIPLYGDLHRFIPALAGIYGAKITELPVRHHPRAQGRSKYTLARTF
                     RVIMDLIIVSFLKSYAQRPMHAFGWAGILMMVTGFGIEGYLAIQKLAFGAQLAGRPLL
                     LFGAVLMLAGLQIASLGILAELQIRTYHESQGKPIYTVREVIASDRNAPG"
     gene            2586..3515
                     /locus_tag="AC16BC_11170"
     CDS             2586..3515
                     /locus_tag="AC16BC_11170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3242219.1"
                     /translation="MKKAGWLALKLAVTVGLLAYLFSRIEVSSVARLMAGAAWGWVVA
                     AFFLYVVLQGLCAWRWLLLARVLNLDGTWKRFVRYYYVGMFFNLFLPTGVGGDVYRCY
                     YVARSAADWRRAIISVLADRGVGFATMCAIAAVATLAFGRVELPSEMGWALGVGVLAL
                     VALLAGGLAARSRFASVWASMPLVVEFFRRPGTLAVVTALSFLLQSLVVVVTVFNAKA
                     LGLDVPLVFYFILIPLIAVATLLPVSLNGLGVREVAFVFFLGQVGVPKDQALSLALLW
                     LVVLTAASAIGGLVWLATPVPRKPPEPGVAPAG"
     gene            complement(3512..>4009)
                     /locus_tag="AC16BC_11175"
     CDS             complement(3512..>4009)
                     /locus_tag="AC16BC_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242220.1"
                     /translation="VFGSWDSKDYNAPFNHRDLTATEVGLSGTVRWRPVAAVQARLAR
                     EIGDAAGAGDATINSDTSYKQWTFAIKPTFSPTPDLDLGLRYEYERRDYTSTLAADAD
                     HFERQDRSNAVGLDGALTVAKKVRLRAAYDHIKRSVTTVAPAANLDFGNYTEQRVTGG
                     MDYRF"
BASE COUNT          485 a         1231 c         1484 g          809 t
ORIGIN      
        1 tcgacgaggc ggtgttcgcc gaggcgaccc gcgagatggt ggcgaccggc gactggatca
       61 cgccgcagta caacggcgcg gaccggttcg acaagccggt gctgttctac tggttgatgg
      121 cgtcggcgta tctggtgttc ggagtgggcg agttcgccgc gcgtttctgg tcggccgcgt
      181 tcggcgtggg cttggtcggg ttgctcgcgg tgttcgcccg gcggcgcctg gggtttcgcg
      241 tcgggctgtt gtccgggctg gtggccgcca cgtcgctcga ggtggtcgtg ctcgcgcacg
      301 cggccattac cgatatggtc ttgatctttt ttctcagcgc cgggctgttg gccttgttcg
      361 cggcggtgca ggcgaccgag gcgggcggcc ggaccgaatc cgcgctgatc agctccgcgg
      421 cgttcggcct ggccgtgctg accaaagggc cggtggggat cgtcctgccg ggcatgatcc
      481 tgctcgtgtt cctcgcgttg cgcggagggt tgagagacgc gctggcgaat ctgccgtggg
      541 gccggatggc gttggtattc gcgttggtcg cggcgccctg gtacgcgttg gaattgcacg
      601 cgcgcgggtg ggcgttcatc gaaggctttt tcctcaaaca caacgtgcgg cggttcaccg
      661 gcgtgatctc ggggcacgcg gggccgttct actattacat ccccgcggtc gcggtcgggt
      721 tcttcccgtg gacctcggtg ttgttgcccg cgatccgctg ggcgtggccg tccggctggc
      781 gcggattgcg cgggccgacc gaccgggagg cgctcgggtt gttcgtgctg gtctggatcg
      841 gggtggtgtt cgcgtttttt tccgcggcgg gcaccaagtt gccgaactac gtggcctcgt
      901 tgtacccgcc gctcgcgctg ctgacgggca tgtgggccga acggttcgtg gcgcgggctc
      961 cgggcgcgga tcggtggatc ggcgcgggca tggcggtcct ggtgggcgtg gccctgatca
     1021 tggccgtggc gttctggggc gtgccgtggt ggttggagtg ggcgcgggtg cgttttccca
     1081 aggccgcgta cctgcaccag cccgttgacg cggggtgggg gcccgcggcg ctcggggcgg
     1141 tgtgcgtgat cggcgcggtc gcgatcgcct ggaccgcgat gcgccgacgt ccgggcgcga
     1201 tgatcggcgc ggtcgcggcc accacggtga ccgccacgat cgtgctgctg accgcggtgt
     1261 ttccggtcgc ggccgacgtc attcaagtcc cgctgcggga tctgtcgatg cgggcaagcc
     1321 gcgaggccgg gcccgacggc gggatcgcgg tctacgggct caacaaaccc agcgtggtgt
     1381 tttacgccaa gcggctggtc accgtggtgg gcgagagccg tccggaggag ttgggcgccc
     1441 tgattcgcga ggatcgcgcg tggttcgtga tcaccaaggc gcggtggctg gaccggctcg
     1501 atctgttcgg tcccattcac gtcgttgaat cgcgcggcgg gtacgtgctc gcgtccaatc
     1561 gcccggccgc gctcggcggc gccgcgccgg gagagacagg aggccgtgat gccggatcgt
     1621 gaacccaaac gccccgcgct gtcgatcgtc attccgttgt tcaacgaagc ggagagcctc
     1681 cccgcgctgg cggagcgggt gttcggcgcg gtcgcccggt acggtttgga cgcggaggtg
     1741 ttgttcgtgg acgacggcag cacggacggc agcgaaggca tctaccgtcg tctgtgcgac
     1801 gtccaccccg cgttgcgggt gatccgcctg cgtcggaact tcgggcagac cgcggccatg
     1861 gccgcggggt tcgaccacgc gcgcggggcc gtgatcgtgg tcttggacgg cgaccttcag
     1921 aacgatcccg acgacattcc gctgctgctc aagaaactgg acgaaggatt cgacgtggtg
     1981 agcggctggc gccgcgaccg ccgcgaccac tggagccggg tgttgccctc gcgtctcgcg
     2041 aactggttga tcggccgcac cacgggcgtg cggctgcacg attacggctg ctcgctgaag
     2101 gcctaccgcg cggagctgat ccagaacatc ccgttgtacg gcgacctcca ccgattcatc
     2161 ccggcgctgg ccgggatcta cggcgcgaag atcacggagt tgccggtccg ccatcaccct
     2221 cgggcgcagg ggcggagcaa atacacgctg gcgcgcacgt ttcgcgtgat catggacctg
     2281 atcatcgtga gttttctgaa gagttacgcc cagcgtccca tgcacgcgtt cgggtgggcc
     2341 ggcatcctca tgatggtgac cggtttcggc atcgaagggt atctcgcgat tcagaagctc
     2401 gcgttcggcg cgcaactggc cggccgtccg ctgctgctct tcggcgccgt gctcatgctg
     2461 gccggattgc agatcgcgag tctgggcatt ctcgcggaat tgcagatccg cacctatcac
     2521 gaatcccaag gcaagccgat ctacacggtc cgcgaagtca tcgcgagcga ccggaacgcc
     2581 ccgggatgaa aaaggccgga tggctcgcgc tcaagctcgc ggtcacggtg ggcttgctcg
     2641 cgtacctttt ctcgcgcatc gaggtgagtt ccgtggcgcg gctgatggcc ggcgcggcgt
     2701 gggggtgggt cgtcgcggcg ttcttcctgt acgtggttct gcaggggctc tgcgcgtggc
     2761 ggtggttgct gctcgcgcgc gtgctcaatt tggacggaac gtggaagcgg ttcgtccggt
     2821 actactacgt cggcatgttc ttcaatttgt tcctccccac gggcgtgggc ggcgacgtgt
     2881 atcggtgtta ttacgtggcg cgctcggccg cggactggcg acgggcgatc atttccgtgc
     2941 tggcggatcg cggggtcggc ttcgcgacca tgtgcgcgat cgccgcggtg gcgacattgg
     3001 ccttcggccg cgtggagctg ccgtcggaaa tggggtgggc cctcggggtc ggcgtcctgg
     3061 cgctggtcgc gctcctggcg ggaggactcg ccgcgcgatc gcggttcgcg tcggtttggg
     3121 cgtcgatgcc gctggtcgtc gagttcttcc gccgtcccgg gacgctggcg gtggtcacgg
     3181 cgctgtcctt cctccttcaa agtctggtcg tggtggtgac ggtctttaat gccaaggcgc
     3241 tggggctcga cgtgccgctg gtgttctact tcatcctgat tccgctcatc gcggtcgcca
     3301 cgctgcttcc ggtaagcctc aatggcttgg gcgtgcgcga agtcgccttc gtgttctttt
     3361 tagggcaggt cggcgtcccg aaggatcagg cgctctcgct cgcgttgtta tggctcgtgg
     3421 tcttgacggc agcgagcgcg atcggcggcc tggtgtggct cgccactccg gtgccccgga
     3481 agccgccgga gccgggagtg gctccggcgg gctagaagcg gtagtccatc ccgcccgtca
     3541 cgcgttgttc ggtgtagttc ccgaagtcca ggttggccgc cggcgcgaca gtcgtcaccg
     3601 accgcttgat gtggtcgtag gcggcgcgga ggcggacctt tttggccacg gtcagggcgc
     3661 cgtccagtcc caccgcgttc gaccggtctt ggcgttcgaa atggtccgcg tcggccgcca
     3721 gcgtggacgt atagtcccgt cgttcgtatt catagcgcag tcccaagtcc agatccggcg
     3781 tgggggagaa cgtgggtttg atcgcgaacg tccactgctt gtacgacgtg tcggaattga
     3841 tcgtcgcgtc gcccgcgccc gcggcgtcgc cgatctcgcg ggccagtctg gcttgcaccg
     3901 cggccacggg gcgccatctc accgtgccgg agagcccgac ctccgtggcc gtcaggtcgc
     3961 ggtggttgaa cggcgcgttg tagtccttcg agtcccacga gccgaagac
//
LOCUS       DBNGTT010000487         5095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_117884, whole genome shotgun sequence.
ACCESSION   DBNGTT010000487 DBNGTT010000000
VERSION     DBNGTT010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_117884"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="AC16BC_11180"
     CDS             <1..1080
                     /locus_tag="AC16BC_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HIR3242221.1"
                     /translation="VAAFAPEWQIREVVLALCVLPFAVILWRTALTKWVRNSLFERKI
                     LLVGNGRLAEDVISALNVTPSFGHTIADRLQSWPLPEYSGEGDRPRSLLKLARDARID
                     HVVVALGDSRGRLPIDDLLQCKFSGLTVEDGLSFYERICGKVHIDALKPSWLIFSEGF
                     KCRYGAVVVKRAFDLVGSAIGLVVLMPLGLLLAALIKLDSRGPVLYRQVRVGEHERPF
                     VLVKFRSMRTDAEQGTGAVWAKRDDNRVTRIGKWLRLLRLDELPQLINVFRGEMSFVG
                     PRPERPEFVEKLKADIPYYGFRHSVKPGITGWAQVRYRYGASIEDTIEKLRYDLCYIK
                     NRTLLLDVSILIETVKIVLMREGAR"
     gene            1077..2018
                     /locus_tag="AC16BC_11185"
     CDS             1077..2018
                     /locus_tag="AC16BC_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HIR3242222.1"
                     /translation="MTAIVNALSVDVEDYFQVSAFDEVVSRAEWDGFESRVDVNTRRL
                     LDMFDRRGVKATFFMLGWVAERHPDLVVEIHRRGHEVASHGYAHRMITQSSPAEFRQE
                     IRQTKAVLEDMIQAPVIGFRAPSFSITQQTEWALEILAEEGFRYDSSIFPIRHDRYGI
                     PRAERFPYVIRYGDRALHEFPMSTVDVFGLTNLPVSGGGYLRWLPLWTTRWGIRRINE
                     HDRRPAVLYLHPWEIDVDQPRIRAGLRSRLRHYRNLKTTTPRLEALLATFRWDTVRAV
                     LGLAVDGELSTAPAFGHPIGRTVPVDPHARPSAVPTG"
     gene            2023..3993
                     /gene="asnB"
                     /locus_tag="AC16BC_11190"
     CDS             2023..3993
                     /gene="asnB"
                     /locus_tag="AC16BC_11190"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3242223.1"
                     /translation="MCGIAGLWNVTSGAPAERIDLDRMVRALHHRGPDDHGIHLAGPL
                     GIGHTRLSIVDLSGGHQPMCNEDGSIWVVFNGEIYNHKELRAGLITKGHRFATHSDTE
                     VILHQYEELGERCVESFNGQFAFALWDAERHRLLLARDRLGVRPLYYVWSRDGIRFAS
                     EIKALFTDPSVPRALDPIALDQIFTFWFPLAPRTGFVGVSEVPPGHTLTVHHGGSTLQ
                     RYWAMEFPAEASGRQPRHPERWYRDRLAELLDDAVALRLRADVPVGAYVSGGLDSSVV
                     AAMARRKVNDRLLTFSIGFEAAEFDERSFQSAVVDHLGTQHTACVYRTLDIGREFPAA
                     MWHMERPVLRTAPVPMMRLAQGVRAAGIKVVLTGEGADEVLAGYDIFKEAKVRRFWAQ
                     SPDSRFRPLLLRRLYPYLSGVQGQASAYLKGFFGQGLEDTSDPFYSHRPRWQVAAQIK
                     SLLAVHVREGLAEYDAVEELRDQLPDAFSTWHPLSQAQYLEAAYLLPGYILSSQGDRM
                     AMAHSVEGRFPFLDHRLVEFAATIPPQFKLKRLREKHLLREVASDLLPSVVIDRDKQP
                     YRAPDHAAFFQDGRPLEYVEAALSPEAVEAAGTFATGPVSGLLAKCRRPGTLGTRDGM
                     ALVGALSAQLLHRQFVERPVGAEVSPHPSEAS"
     gene            4009..>5095
                     /locus_tag="AC16BC_11195"
     CDS             4009..>5095
                     /locus_tag="AC16BC_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3242224.1"
                     /translation="MQVESFLEQSAREYPDKAALVSPQGRFTYAEVDRAAIRLAHALR
                     EQGVRRGDRVAIYLENSAEAVVAVFAVLKLGAVFVLLNPGIKADKLAFILNDCRAVGL
                     VASSQGRIAGEVLDRTSSARFLIVVGEPAGTSIPGVAVIGWDACLLDRDQPAIRGAGI
                     DLDLATIIYTSGSTGFPKGVMMTHLNMVTAATSISQYLEIRRDDVILNVLPLSFDYGL
                     YQILMAFKAGATVVLERSFTYPAAVIETLTKEAITGLPVVPTLATMLIRMGGLMPGQF
                     PSVRYITSTAAALHPAVISELRRLFPAAKIFSMYGLTECKRVSYLPPDQLDARPTSVG
                     KAIPNTEVWLVNDRGERVGPGTVGELVV"
BASE COUNT          844 a         1541 c         1700 g         1010 t
ORIGIN      
        1 gtcgcggcat ttgcgccgga gtggcagatc cgggaagtcg tactcgctct gtgcgtgttg
       61 ccgtttgccg tgattttgtg gcggacggcg ttgaccaagt gggtgaggaa ctcgctgttc
      121 gagcgaaaaa tcctcctggt gggcaacgga cgacttgccg aagacgtgat ttcggcgctg
      181 aacgtgacgc cgagtttcgg ccacactatt gcggaccgtc ttcaatcctg gccccttccc
      241 gagtattcgg gagaaggcga tcgcccgcgt tcgctgctca aactggcgcg tgacgcccgg
      301 atcgatcacg tcgtggttgc tctgggcgac agtcgcgggc gtttgccgat cgatgacctg
      361 ctgcagtgta agttttccgg cctgaccgtc gaagacggat tgtcgttcta cgagcggatc
      421 tgcggaaagg tgcacatcga cgcgctcaaa ccgagttggc tcattttttc ggaagggttc
      481 aagtgccggt acggcgccgt ggtcgtgaag cgggcgtttg atctggtcgg atccgcgatc
      541 gggctggtcg tgctcatgcc gcttggcctg ctgctggccg cgctgatcaa gctcgactcg
      601 cgcggccccg tactgtatcg gcaagtgcgc gttggggagc acgagcggcc gtttgttttg
      661 gtcaaattcc gttcgatgcg cacggacgcg gaacagggca cgggtgcggt ctgggcgaag
      721 cgcgacgaca accgcgttac tcgaattggc aaatggttgc gtctgctgcg tcttgacgag
      781 ctgccccagt tgatcaacgt gttccggggc gaaatgagtt tcgtggggcc gcggccggag
      841 cgcccggagt tcgtggaaaa attgaaggcc gacattccgt actacggctt ccgacactcc
      901 gtgaaacccg gcatcacggg atgggcccag gttcgttatc gctacggcgc cagcatcgag
      961 gacacgatcg agaagctccg ttacgatctc tgctacatca aaaatcggac gctcttgttg
     1021 gacgtgtcga tcctgattga aacggtcaag atcgtgctca tgcgggaggg ggccaggtga
     1081 cggcgatcgt caacgccctg tcggtcgacg tcgaggacta cttccaggtc tcggcgtttg
     1141 acgaggtcgt gtcgcgcgcg gagtgggacg gatttgagtc ccgcgtggac gtgaacaccc
     1201 ggcggttgct cgatatgttt gatcgccgcg gggtgaaagc gacctttttc atgctgggct
     1261 gggtggccga gcggcatccg gatcttgtcg tcgagattca ccgccgcggc catgaggtcg
     1321 caagccacgg atacgcccac cgaatgatca cccagtcgtc tccggccgaa ttccggcagg
     1381 agatccggca gacgaaggcc gtcctggagg acatgattca ggccccggtg atcgggttcc
     1441 gcgcgccgag tttctcgatc acgcaacaga ccgagtgggc gctcgagatt ctcgcggagg
     1501 aaggcttccg atacgattcc agtattttcc cgattcgcca cgatcgctac gggatcccgc
     1561 gggccgagcg gtttccctac gtgatccggt acggcgatcg ggcgctccac gagttcccca
     1621 tgtccacggt cgacgtattt ggactcacca atctgccggt gtccggcggg ggctatctgc
     1681 ggtggttgcc gctgtggacg acgcgatggg gaatccgtcg gatcaatgaa cacgaccgcc
     1741 ggcccgccgt gttgtacctg catccctggg aaatcgacgt cgaccagccg cggattcggg
     1801 ccgggctccg ttcgcgactg cgacattacc gcaacctgaa gaccacgacg ccgcgattgg
     1861 aggcgttgct ggcgacgttt cggtgggaca ccgtgcgcgc ggtgttgggg ctagcggtgg
     1921 acggcgaact ctccacggcc cccgcgttcg ggcatccgat cggccgaacg gtcccggtcg
     1981 acccccacgc gcggccgagc gcggtcccga ctgggtagca cgatgtgtgg aatcgccggt
     2041 ctgtggaacg tcacctccgg agcgccagct gaacgaatcg atttggaccg aatggtccgg
     2101 gcgctgcatc accgcggacc ggacgatcac gggatccacc ttgccggtcc gctcgggatc
     2161 ggccacacgc ggctgagcat cgttgatctg tccggcggac accagccgat gtgcaatgag
     2221 gacggctcga tctgggtcgt ctttaacgga gagatctaca accacaaaga acttcgcgcc
     2281 ggcctgatca cgaagggcca ccggttcgcg acccactcgg acaccgaggt tatcctgcat
     2341 cagtacgaag agctcggtga acgctgcgtg gaatcgttca acggccagtt cgccttcgcg
     2401 ctctgggacg ccgaacgaca tcgtttattg ttggcgcgcg accgtctcgg cgtgcgcccc
     2461 ttgtactacg tgtggagccg ggacgggatc cggttcgcgt ccgagatcaa ggcgctgttt
     2521 acggatccgt cggtgcctcg cgcgctcgac cccatcgccc ttgaccagat tttcacgttc
     2581 tggttcccct tggcgccccg caccggtttt gtcggcgtct ccgaagtgcc tcccggtcac
     2641 acgttgacgg tccatcacgg cggaagcacg cttcagcggt actgggcgat ggagtttccg
     2701 gccgaggcat ccggccggca gccgaggcat ccggagcgct ggtatcggga tcgcttggcg
     2761 gaattgctcg atgacgccgt ggcgttgcgg ttgcgggccg acgtgccggt cggcgcctac
     2821 gtcagcggcg gactcgactc gtcggtggtc gcggccatgg cccgtcgcaa ggtcaacgac
     2881 cggctcctga cgttttcgat cgggtttgaa gccgcggaat tcgacgaacg gtcattccag
     2941 tccgcggtgg tcgatcacct cggaacgcag cacacggcat gcgtctatcg cacgctcgac
     3001 atcggccggg aattccccgc ggcgatgtgg cacatggagc gtccggttct gaggaccgcg
     3061 ccggtgccca tgatgcggtt ggcgcaaggg gtcagagcgg ccggcatcaa ggtcgtgctg
     3121 accggggagg gcgcggacga ggttctcgcc ggatacgaca tcttcaaaga ggccaaggtt
     3181 cgccgattct gggcgcagtc gccggactcg cggttccgcc cgctcctgct tcgtcgcctc
     3241 tatccgtatc tttccggcgt tcagggacag gcctccgctt atctgaaggg attcttcgga
     3301 cagggactgg aggacacgtc ggatccgttc tattcgcatc ggccgcgctg gcaggtcgcg
     3361 gcgcagatca agagtctcct ggccgttcac gtccgcgaag gtctcgcgga atacgacgcg
     3421 gtcgaggagc ttcgagatca attgcccgac gcgttttcaa cgtggcaccc cctgtcgcag
     3481 gcgcagtacc tcgaggccgc gtatttgctc ccggggtata tcctgtcgtc gcagggcgat
     3541 cgcatggcga tggctcattc ggtcgaagga cgatttccgt ttttggacca tcgtttggtg
     3601 gaattcgccg cgacgattcc cccgcagttc aagctcaaac gattgcgcga gaaacatctc
     3661 ctccgcgagg tcgcgagcga cctgttgccg tccgtcgtga tcgaccgcga taagcaaccg
     3721 taccgcgcgc cggaccacgc cgcgttcttc caggacggac gacccctgga atacgtggaa
     3781 gccgcactgt cgcccgaagc ggtggaggcg gccggcacct tcgcgaccgg tccggtcagc
     3841 gggctcctgg caaaatgccg gcgcccgggt acgctgggca cgcgcgacgg gatggcgttg
     3901 gtcggcgcgc tgtcggcgca attgctgcac cgacaattcg tggagcgccc ggtgggagcc
     3961 gaggtgtcgc cgcacccctc cgaggcctcg taaacggcgg gaggtccgat gcaggtcgaa
     4021 tcgtttctcg aacagtccgc gcgcgaatac cccgacaagg cggcgctggt gtctccacag
     4081 gggcggttca cctacgcgga ggtggaccgg gccgcgatcc gtctcgctca cgcgttgcgt
     4141 gaacaggggg tgcggcgcgg cgatcgcgtc gcgatctatc tggagaattc ggccgaggcc
     4201 gtcgtggcgg tgttcgccgt gctgaaactg ggcgcggtgt tcgtgttgct caacccgggg
     4261 atcaaagcgg acaaactggc gttcattctc aacgactgtc gggcggtcgg tctggtggcc
     4321 tcgtcccagg gccggatcgc gggggaggtg ctcgatcgaa cgtcatcggc gcggtttctg
     4381 atcgtcgtcg gtgaacccgc ggggacctcg attcctggcg tcgcagtgat cggatgggac
     4441 gcgtgcctgc tcgatcgcga tcagccggcc atccggggcg cgggcatcga cttggatctc
     4501 gcgaccatca tctacacctc gggatcaacg ggtttcccga agggggtcat gatgacccat
     4561 ctcaacatgg tgaccgccgc gacctcgatc tcccagtacc ttgaaattcg ccgcgacgac
     4621 gtcattctca acgtgctgcc gctgtccttc gactacgggc tctatcagat cctgatggcg
     4681 ttcaaagccg gggccaccgt ggtgttggaa cggtcgttca cttatcccgc ggcggtgatc
     4741 gaaacgttga ccaaagaggc cattaccggt ttgccggtgg ttccgaccct ggcgacgatg
     4801 ctgatccgga tgggcggctt gatgcccgga caattcccgt cggtccgata catcacgagc
     4861 acggcggcgg cgctccatcc cgcggtaatc agcgaactcc gacgattgtt tccggcggcc
     4921 aagatctttt cgatgtacgg gctgacggag tgtaaacggg tgtcgtatct cccgcccgat
     4981 cagttggacg cgcggccgac gtcggtcggc aaagccatcc ccaataccga agtatggttg
     5041 gtgaacgatc ggggcgagcg cgtggggccc ggcacggtgg gcgaactggt ggtgc
//
LOCUS       DBNGTT010000488         2076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_885053, whole genome shotgun sequence.
ACCESSION   DBNGTT010000488 DBNGTT010000000
VERSION     DBNGTT010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_885053"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC16BC_11200"
     CDS             <1..612
                     /locus_tag="AC16BC_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242225.1"
                     /translation="GGDVPVVLGSAGNPSGHRVHFFAKSYAATLIDPSRQASVAAEFG
                     IERFAKAYGLEPLGTGIGLGMVTRYDAYLCVYEADSLSQIGLTEQTTSDPEAADVDEA
                     LDVLAGSDGARVCLMIPYGYVLGVLRFDPARRRVVITDGDIDSPSYTVIRSGLTIKST
                     TSRVIPKAIERELGTAPSHVYEIHRHCGWKERIEYVGRIHVCA"
     gene            636..1091
                     /locus_tag="AC16BC_11205"
     CDS             636..1091
                     /locus_tag="AC16BC_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242226.1"
                     /translation="MTRTSVIGLMLAGLTCAVVVLSAPRAACAATDEEVRMIREIEEL
                     RETIATTEWTWWNRDQKKPLEDRIALLTRILDRVAQRGSDLRRYPEGTSRRHKRGSVT
                     NEDVQLIREAEEIREEIRATEWTWNNRDLKTALEKRLDLVEKILSRSGP"
     gene            complement(1383..1661)
                     /locus_tag="AC16BC_11210"
     CDS             complement(1383..1661)
                     /locus_tag="AC16BC_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3242227.1"
                     /translation="MKKIRAADFVPAKPRASVTTGEVIKMLRELKGWTQKELATRCGI
                     SPSNLSQLEHDRLEIGKKRAQQLATVFGVHPAIIMFPEYEAAEITRVA"
     gene            complement(1940..>2076)
                     /locus_tag="AC16BC_11215"
     CDS             complement(1940..>2076)
                     /locus_tag="AC16BC_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3242228.1"
                     /translation="FCGTQNITSDVEVIRIQDINEAYQRMLKSDVKYRFVIDMSSLKA
                     "
BASE COUNT          415 a          598 c          651 g          412 t
ORIGIN      
        1 ggcggcgacg tgccggtggt tcttggttcg gcgggaaatc cttcaggcca ccgagtccat
       61 ttcttcgcca aatcgtacgc cgcgacgctg atcgatccgt cgcggcaggc gagcgtggcc
      121 gcggaattcg gcattgaacg gtttgccaag gcgtacggcc tggagccgtt ggggaccggg
      181 atcgggctcg gcatggtgac gcgctacgac gcgtatctct gcgtgtacga agccgacagt
      241 ctctcccaaa tcggactgac cgaacaaacc acgtcggacc ccgaggccgc ggacgtggac
      301 gaggcgctgg acgtgctcgc gggttcggac ggcgcgcgcg tgtgtttgat gattccctac
      361 ggctacgtcc tcggcgtgct ccgcttcgac cccgcccgcc gccgcgtggt catcacggac
      421 ggcgacatcg acagtccctc atacaccgtc atccggtcgg gtctcaccat caaatccacc
      481 acctcgcgcg tgattcccaa ggcgattgag cgcgaactgg gaacggcgcc gtcccacgtc
      541 tacgagatcc accggcactg cgggtggaag gagcgaatcg aatacgtggg gcggatccac
      601 gtctgtgcgt aacaggcggg aggcgtacga tgcccatgac gagaacgagt gtcatcggcc
      661 tgatgttggc cggtctgacc tgcgccgtgg tggtgttgtc cgcgccgcgc gccgcatgcg
      721 cagccacgga cgaggaagtc cgcatgatcc gggaaattga ggaactgcgg gagaccatcg
      781 cgacaacgga gtggacgtgg tggaaccggg atcaaaaaaa gccgttggaa gaccggatcg
      841 cattgttgac cagaattcta gatcgggtcg cgcagcgcgg gtcagacctc cggcggtacc
      901 ccgaggggac atcgaggcgg cataagcggg gttcggtcac gaacgaggat gtgcagttga
      961 tccgggaagc cgaggagatc cgagaggaaa tcagggccac ggagtggacc tggaacaatc
     1021 gcgacctcaa gaccgcgttg gagaagcgcc tcgacctcgt ggagaagatc ttgtcccgaa
     1081 gcgggccgtg aagatgtcgc ggtaacccgc atcatgtcca tcatcacggc gttcatcgcg
     1141 gaataattgg ggtcagaacg agagtgaaaa aatggggacg gatttattta gctgaaccgc
     1201 tcgaaccggt acaccagttc tgggcccctt ttccctcgta cgcgagttgg gcgacgagca
     1261 gcgcgaacgg ctcggcgagg agcggtcggg gtcacgagga gcggtcgcgt cgcatccggt
     1321 gtcgaatctt taattgttag tgatacgcgt gttccccttc cgctgctcgc atattacgga
     1381 tgtcacgcca cccgggtgat ctcagcggct tcgtactcgg ggaacatgat gatcgccgga
     1441 tggacgccga agaccgtcgc caattgttgg gctctctttt tcccgatttc gaggcggtcg
     1501 tgttccaact ggctgaggtt gcttgggctg atcccgcagc gcgtcgccaa ttctttctga
     1561 gtccatccct tcagctcccg caacatctta atgacctcgc cggtcgtcac gctcgcgcgg
     1621 ggcttggcgg gcacgaaatc ggctgctcga atcttcttca tcttcaggct cctagtaccg
     1681 atgtggcgtg agttctagaa cgtaaaccac gaagtcgtga tcgtcgactt gatatagctt
     1741 ctcaaaaaat gagaagtcaa ggcggcgctg ttttcggaaa agcgtggcgg tggcgatcat
     1801 cgccgcccct ggggaggcgg atccgaaaca agatctgggg ggcaggtctt gcaatccacc
     1861 tatggcgcga agagcgtccc gagcgtgccg tccatcaacc ccgaacccca aacaccgacg
     1921 cgcatgatgc tcctccatct caggccttca gcgatgacat atcgatcaca aagcggtatt
     1981 tcacgtcact tttcagcatt cgctgatacg cttcattgat gtcctgaatt ctgatcactt
     2041 cgacatctga agtgatgttc tgcgtgccgc agaagt
//
LOCUS       DBNGTT010000489         8805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_118536, whole genome shotgun sequence.
ACCESSION   DBNGTT010000489 DBNGTT010000000
VERSION     DBNGTT010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_118536"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3016)
                     /locus_tag="AC16BC_11220"
     CDS             complement(<1..3016)
                     /locus_tag="AC16BC_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3242229.1"
                     /translation="MKNLGRALWGLGLGVAVLAGQPGKVFAGPGHEGEHADHAESAAT
                     TQVADAGWEAKLKAQIEREDTAEGRSGHRDRVDAAMQKLMQEIAKGTEDHSAHTGGGA
                     FSGMGMMQQMDRSYFLGPASGESVSTGGRCPKGAPVKAYDISAINVEITLNQWLDFHS
                     GYMYVLTENIDKVRQEEKINREARKKPGYDPGAVTTGMQTDMIQPLNIRGNQGDCIVV
                     TLRNALDGEDVGMHVHGSSFIVQSTGAPATAANPDANVAPGKSQVFEWYIGPTEQEGV
                     HMLHSHVGREQASLGMIGSFIVEPMGSTYIDPISGKEAKSGWQMMVAHPDTPDFREFV
                     IFYHEIGDESFRPLNRSGETIPQRDPNTDAYRPSARAISYRSEPFGVNNLALQEKYFH
                     HEDESLSYSSYTFADAPTTIPRAYMGDPVKWRLVHGGGEVFHSHHPHGGTIRWLRQPK
                     ADGKSEFLLTAAQNGPVKFPEVRTTSDRVDVQVIGPTEVVDLQNECGSGLCQQLAGDF
                     LFHCHVAHHYVAGMWGYGRTYNTLQVGNYPMGSTDVMPPLLELPDRKGRMKPAVTSDQ
                     LIGKTMDWYDKKWNITANKSDWSKPIPDVSIKDWVKMMVPPAGQPGHTADEKGQITAY
                     DATVWDWTWDGNKALGEPEATGTFDHPKYKSPMPGKRPPILFDAKTGKLAWPHFKPHF
                     GKRVPFARHHGPAPWLEPIHMAKDNGALASDPGSKGAVGDETNQPAKPGEQGRWSLCP
                     ENAGRKQYTIHFIQTGIQLTKGLGKQQPVVDKNGLLYVLKENEDEIRANPAGDKTIPL
                     VYRANVYDCVDVILKSEWVDNDTTNFQMSKINIHPHFIQFDNQASDGVISGFSYEQSV
                     RPFVQMHKEKHKGMPTPMNALLTEEAKAGSTSIKIKMGEGATPFHVGTDLMIGADEVK
                     TSEVLWIKDIKGDTVTFTEPLRFDHKKDEIATVEFVRYRMWVDSDIGTVFWHDHAFGA
                     TTWPHGGVGAMIVEPYGSTYHDPKTGAPIRSGP"
     gene            3692..4180
                     /locus_tag="AC16BC_11225"
     CDS             3692..4180
                     /locus_tag="AC16BC_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242230.1"
                     /translation="MAAGPIPSCDDQAGATITIEMKVAKQWRPRTAEIKRELQSGPGS
                     VNARVEVTAMAAPPLNIGIGRCVDAEAARLAIRMAKTYNGGIDRLIFQEILPHRWIMI
                     GTTQVAELSWTPVSPEELAQLADPSLSTEAFQTLYRTLATPKERKRPFGMGPVPAQPA
                     PK"
     gene            complement(4214..6250)
                     /locus_tag="AC16BC_11230"
     CDS             complement(4214..6250)
                     /locus_tag="AC16BC_11230"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HIR3242231.1"
                     /translation="MKRAAVGPLAVSVALAFTAATAAAAQLPSSAEPLLINQIAVAPS
                     NPDVLYAAGRPAGVLKSTDRGRTWRPARDGILNTSAYHVVVHPTNADTVYVGTYGGGV
                     YRTRDGGRSWDAASAGLGNANIHALYLDPSRPERLIVSTSTGDVFQTLDEGARWTAYG
                     QGLPAMEGEVLAVFLSRPNDAWLGQGTLYSRGARDPAWRAVGQDLGAAAITVLAADAD
                     GRLYAGTRRQGLLRSIDNGATWSDVAPEFDRRWIRLVLPGPNGRVWVSALRRGLYRSD
                     DRGATWIKVGAGLPENDDVETLAMDPADPNRLYAGTHDHGLWLSSDGGRGFAPPDHVM
                     QERVADIIASLIDRPVKPGASQAPAVPSVFQKCSGCHGWTDPALSRKPTYWRVAANQR
                     DWAPTVARMAPGAGLNAVEMWEIVAFLTRYTEQKGDRLLFREKVACPLCLVEIPAGWF
                     LMGSEPRDPTAGLWLPYDNTESPQRRIWLDPYAIDREEVRVADYMRWRRAQPDAAGTE
                     SSPIDAEVGARPMVGVTWSEADRYCRAMGRRLPTEAEWEKAARGAAGRLFPWGDDPPD
                     ARRAVFGRPASDLGEIAAVDALLDGRSPYGVLNMAGNAAEWVADWSGIDWYAVMPERN
                     PKGPTSGRYKVVRGGSWRSDPVMLRAATRSAAEPETRRDTVGFRCAGDPPGVPP"
     gene            complement(6247..7374)
                     /locus_tag="AC16BC_11235"
     CDS             complement(6247..7374)
                     /locus_tag="AC16BC_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242232.1"
                     /translation="MGPEIVISSRAADEEEDPVPSWNGSRFLVVWQSTRRGPSDIYGA
                     WVAPDGAVAPPEGFPVGAGPSDTLFPDVAWGGDTHLAVWQDLRSRRNWEIFGARVRPD
                     GTIVDPDGLKVGAGEGNRRHPRVAWSGRVFLAVWMQERPGSGWDIVATRITPDGKVLD
                     PDPIAVGGGPGDQTRPAVAWSGRAFVVAWMDGRRGDNDIATARVDDDGRVLDPEPITV
                     CAAPGEQGYPAVGGLVASGESVIAWVDKRAGDRYALYGATLGPDGRVGQPDGVALSAA
                     PRLHMFPAATCRTQDCLIVWEEEQPSTGRMTAVTDLVRDVRALRWTGGAAKPVPFTVE
                     PLAKGNHFTTVAAGGPGYLVAWKDYRTGRAEARARLIEFPP"
     gene            complement(7562..8533)
                     /locus_tag="AC16BC_11240"
     CDS             complement(7562..8533)
                     /locus_tag="AC16BC_11240"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3242233.1"
                     /translation="MMQERWTSWVVRAAGCWMAAGLIGCAAARGPVLYVADPGGDSVA
                     LVDPDGRAAVARIGDIPKPWDVTVSPDGKTVYVGGQGRTVAAVHADTRRVDRVEAGAT
                     PQAVDVSPDGRALFVANLTDQTLSVISTTTGRLLAQPRVGARPVAVAGIPRRPAVYVV
                     NYFAGTVSVVSMATYAVVGTIPVGMFPTEIAVPADGGFAYVTNQMPGSVSVIDTARDV
                     VVATVPVGQNPAGIALSPDGQIAYVANSGSDTLSIVSTATDREVAVVPVGAVPWGVAV
                     ALDGTRVYVTHRETGRIAVIDAAERRVTGWLESGAVDGLLGIAVVPR"
     gene            complement(8530..>8805)
                     /locus_tag="AC16BC_11245"
     CDS             complement(8530..>8805)
                     /locus_tag="AC16BC_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242234.1"
                     /translation="ADAIITSLKVRDAGRPPSPLVPPAAFAKCNGCHGWTDPEINLAP
                     HSFWLVPPNRRDWRQTVHRTAPIANLSTAEEDEVAAFLTAYSSQERP"
BASE COUNT         1396 a         3193 c         2727 g         1489 t
ORIGIN      
        1 ccggtccgct gcgaatcggc gcgccggtct tgggatcgtg atacgtcgag ccgtagggct
       61 ccacgatcat ggcgccgacc ccgccgtgcg gccaggtcgt ggcgccgaac gcatggtcat
      121 gccagaacac ggtcccgatg tcggaatcca cccacatccg gtaccgcacg aattccacgg
      181 tggcgatctc gtccttctta tggtcgaacc gcaggggttc ggtaaaggtc acggtgtcgc
      241 ccttgatatc cttgatccag agcacttccg acgtcttcac ttcgtccgcg ccgatcatca
      301 gatcggtgcc gacgtggaac ggggtcgcgc cctcgcccat cttgatcttg atggacgttg
      361 atccggcctt ggcctcttcg gtcaacagcg cgttcatcgg ggtcggcatg cccttgtgtt
      421 tctctttgtg catctgcacg aacgggcgca ccgactgctc gtacgagaac ccgctgatca
      481 cgccgtccga cgcctggttg tcgaactgaa tgaagtgcgg gtggatgttg atcttggaca
      541 tctggaagtt cgtggtgtcg ttgtccaccc actcgctctt gagaatcacg tccacgcagt
      601 cgtacacgtt ggcgcgatag accaagggga tggtcttgtc tccggccggg tttgcgcgaa
      661 tctcgtcctc gttttccttg aggacgtaca gcaagccgtt cttgtcaacc acgggttgct
      721 gcttgcccag ccctttggtc aactgaatgc cggtctgaat aaagtgaatg gtgtactgct
      781 tgcgccccgc gttttccggg cagaggctcc accggccctg ctcgccgggt ttggccggtt
      841 ggttcgtttc atcaccaacc gcgcccttgc tccccgggtc gctcgccaat gcgccattgt
      901 ccttggccat gtggatcggt tccagccacg gcgcaggacc gtggtggcgc gcgaacggca
      961 cacgcttgcc gaagtgcggc ttgaagtgcg gccacgccag tttacccgtc ttggcgtcga
     1021 acaggatcgg cggacgcttg ccgggcatcg gcgacttgta cttcggatgg tcgaaggtgc
     1081 cggtcgcttc cggctcgccc aacgccttgt tgccgtccca cgtccagtcc cacacggtcg
     1141 cgtcgtacgc cgtgatctgg cccttctcgt ccgcggtgtg gcccggctgt ccggccggcg
     1201 gcaccatcat cttgacccag tccttgatcg acacgtccgg gatcggcttg gaccagtcgg
     1261 acttattcgc ggtgatgttc cacttcttgt cgtaccaatc catggtcttg ccgatcaact
     1321 gatcggacgt cacggccggc ttcatccggc ccttgcgatc cggcagttcc agcaacggcg
     1381 gcatcacgtc cgtgctgccc atcggataat tgccgacctg caacgtgttg tacgtccggc
     1441 cgtatcccca catcccggcc acgtagtggt gcgccacgtg gcagtggaac aggaagtcac
     1501 cggccagctg ctggcacaac ccggacccgc actcgttttg gagatcgacc acttcggtcg
     1561 gcccgatcac ctgcacgtcc acgcggtccg acgtggtgcg gacttctgga aatttgaccg
     1621 gcccgttctg cgccgcggtc aacaagaact cggatttgcc gtcggccttg ggctgacgga
     1681 gccagcgaat ggtcccgccg tgcgggtggt gtgagtgaaa cacctcgccg cccccgtgca
     1741 ccaaccgcca tttgaccggg tcgcccatgt aggcgcgggg gatggtggtc ggcgcgtccg
     1801 cgaacgtgta cgagctgtac gacagcgact catcttcgtg atggaagtac ttttcctgca
     1861 atgccaggtt gttcacgccg aacggctcgc tccgatagct gatggcgcgc gccgaggggc
     1921 gatacgcgtc cgtgttcgga tcgcgctgcg gaatggtttc cccgctgcgg ttcaacggac
     1981 ggaacgactc gtcgccgatc tcgtggtaga agatcacgaa ctcgcggaag tccggcgtgt
     2041 ccgggtgcgc gaccatcatt tgccacccgc tcttggcttc cttgccgctg atgggatcga
     2101 tgtacgtgga ccccatcggc tccacgatga aggacccgat catgcccagg ctcgcctgct
     2161 cgcggcccac gtggctgtgc agcatgtgca cgccttcctg ctcggtcggg ccgatgtacc
     2221 actcgaacac ctggctcttg cctggcgcca cgttcgcgtc cgggttggcc gcggtcgcgg
     2281 gcgccccggt cgactgcacg atgaagctcg atccgtgcac gtgcatgccc acgtcttcgc
     2341 cgtccaacgc gttgcgcagg gtaaccacga tgcaatcgcc ctgattgccg cggatgttga
     2401 gcggctggat catgtcggtc tgcatcccgg tggtcacggc gccgggatca tatccgggct
     2461 tcttgcgcgc ttcccggttg atcttttcct cctgccggac cttgtcgatg ttctcggtca
     2521 acacgtacat gtagcccgag tggaaatcga gccactggtt gagcgtgatc tccacgttga
     2581 tcgcggagat atcgtacgct ttgaccggcg cgcccttggg gcagcgaccg ccggtggaga
     2641 ccgactcgcc cgaggcaggg cccaggaagt agctgcgatc catctgctgc atcatgccca
     2701 taccgctgaa cgccccgccg ccggtgtggg ccgaatggtc ctcggtgccc ttggcgatct
     2761 cttgcatgag cttctgcatc gccgcgtcca cccggtcgcg atgtccggac cggccctcgg
     2821 ccgtgtcttc gcgctcgatc tgggccttga gcttggcctc ccagccggcg tccgccacct
     2881 gcgtcgtcgc ggcggactcg gcgtgatccg cgtgctcgcc ctcatgaccg ggaccggcga
     2941 acaccttgcc cggctgtccg gccagcaccg ctacacccaa cccgaggccc catagggcac
     3001 gtccgagatt cttcatgcca ctcccccctt ttccctgttg ctgaacgctt gacctgcggt
     3061 gtccataccg gccgttcccg actcgactcg tcggaggccg acaacgaacg ctctatgcaa
     3121 ttcgagctgt gaggaatcgg gccatccacg accgaccacg gaattcatct cgctcgttcg
     3181 gatcaatttc cgcactactt tcgaggcagg aacgcgggac actgattctt gccgacaacc
     3241 agttggtggt gtagagcaca tcggcccgcg ggatcgggcc gtttcacggc tgggagggtc
     3301 gattaacacg atcgaacaac tactcgttgc gcatcacctt gttcggaacc ggtcatttcg
     3361 ctcgaaagta gcaattcgag cgcctcctta tactcggatt gtcctctggc tgtcaagcaa
     3421 aaacttggac atgtacagaa ctttcgacag tcgatccgat cgtccttgtc gatcggtttg
     3481 agatcatcat cgaaacagga tatgatcacc gcctcagtac catgccccga ccaatcaata
     3541 cacgacgacc acaccatcaa ccccatcacc ccgatcgccg agcatgaacg caaatcgatt
     3601 ccctggagca cccgccgcgc ctcgggcgtg gattccgttg cacagcatct tcctcgtact
     3661 cggactcggc attggaggag gaacggagtc gatggccgcg ggtccgattc cgtcttgcga
     3721 cgatcaagcc ggcgccacaa taaccattga aatgaaggtg gccaaacagt ggcgaccgcg
     3781 gaccgcggag atcaaacgcg agttgcaatc cgggcccgga tcagtcaacg cgcgggtgga
     3841 agtcacggcc atggccgccc cgcccctgaa catcggcatc ggccggtgcg tggacgccga
     3901 ggccgcgcgg ctcgcgatcc gaatggccaa gacctataac gggggcatcg accgcctgat
     3961 ctttcaggag atcctgccgc atcgatggat catgataggg accacgcagg tggcggagct
     4021 gtcctggacc ccggtcagcc ccgaagaact cgctcaactg gccgacccct cactctcgac
     4081 cgaggcgttc caaacgttgt accggacctt ggccacgccg aaggaacgca agcgcccctt
     4141 cggcatgggc ccggttcccg cgcagcccgc ccccaaataa ggcccgcggg ttccgcggca
     4201 accgtgacaa cgcttacggt ggcacgcccg gcggatcgcc cgcgcaccga aacccgaccg
     4261 tatcgcgccg cgtctccggc tccgcggcgc tgcgggtagc ggcgcgcagc atgacgggat
     4321 cgctgcgcca ggacccgccg cgcaccacct tataacgccc gctggtcggt cccttgggat
     4381 tgcgttccgg catcaccgcg taccagtcga taccgctcca gtccgcgacc cactcggccg
     4441 cgttgccagc catgttcaac accccgtaag gactccgccc gtccagcaac gcatcaacgg
     4501 ccgcgatctc gccgaggtcc gacgcgggcc gtccaaacac cgcgcggcgc gcgtccggcg
     4561 gatcgtcgcc ccagggaaac agccggccgg ccgcgccgcg cgccgccttc tcccactccg
     4621 cctcggtcgg cagacgccgg cccatcgcgc gacaatagcg atccgcctcg ctccacgtca
     4681 cccccaccat cggtcgggcc ccgacttcgg cgtcgatcgg cgacgactcc gtcccggccg
     4741 cgtccggctg ggcccgccgc caccgcatgt agtccgccac ccggacctcc tcgcggtcga
     4801 tggcgtaggg atccagccag atccggcgct gcggactttc cgtgttgtcg tacggcagcc
     4861 acagccccgc ggtgggatca cgcggctccg accccatcag aaaccagccc gcgggaatct
     4921 cgaccaaaca aaggggacag gctacttttt cacgaaaaag tagcctgtcc cctttttgct
     4981 ccgtgtaccg cgtcagaaac gcgacgatct cccacatctc cacggcgttg agcccggcgc
     5041 cgggcgccat gcgcgccacg gtgggggccc agtcccgctg attcgcggcc acgcgccaat
     5101 acgtgggttt ccggctgagc gccgggtcgg tccacccgtg gcaccccgag cacttctgaa
     5161 acacggacgg gacggcgggc gcttgcgagg cgccgggctt caccggacgg tcgatcaacg
     5221 aggcgatgat gtccgcgacg cgctcctgca tgacgtgatc cggcggcgcg aaccctcgcc
     5281 cgccgtctga cgagagccac aggccgtggt cgtgcgtgcc ggcatacagg cgattcgggt
     5341 cggccgggtc cattgccagg gtttccacgt cgtcgttctc cggcaacccg gcgccgacct
     5401 tgatccacgt ggctccgcga tcgtcgctgc ggtacagccc gcggcgcaac gcgctcaccc
     5461 agacccggcc gttcgggccg ggcaggacga gccgaatcca tcgacggtcg aattccggcg
     5521 ccacgtcgga ccaggtcgcc ccgttgtcaa ttgaccgcag caggccttgg cggcgcgtgc
     5581 ccgcgtagag gcggccgtcc gcgtccgcgg ccaataccgt gatcgccgcg gcgccgaggt
     5641 cctggccgac cgcgcgccac gccggatcgc gcgccccgcg cgaatacagc gtgccttgtc
     5701 ccagccaggc gtcgttgggc cgtgagagaa agaccgcgag cacctcccct tccatcgcgg
     5761 gcagcccctg gccgtacgcg gtccaccgcg cgccctcgtc gagcgtttgg aacacgtcgc
     5821 ccgtcgaggt cgatacgatc aggcgttcgg gacgcgacgg atccagatac agggcgtgga
     5881 tgttggcgtt gccgagcccc gcgctcgcgg cgtcccaact ccgccccccg tcgcgcgtgc
     5941 ggtacacccc gcccccgtag gtgccgacat agaccgtgtc cgcgttcgtc gggtggacga
     6001 ccacgtgata cgcgctggta ttgaggatgc cgtcgcgcgc gggccgccac gtgcggccgc
     6061 ggtccgtgct cttgagcacg cccgcggggc ggcccgccgc gtacaacacg tcgggattcg
     6121 acggcgccac ggcaatctgg ttgatgagca gcggttcggc gctcgacggc aactgggccg
     6181 cggccgccgt cgccgcggtg aacgcgagcg cgacggagac agcgagcgga cccaccgcgg
     6241 cgcgcttcac ggcgggaatt cgatcaggcg ggcgcgcgct tcggcccgtc cggtgcggta
     6301 gtctttccac gccaccagat agccgggccc gcccgccgcc accgtggtga agtgattgcc
     6361 cttggccagc ggctcgaccg taaacggaac cggcttggcc gcgccgccgg tccatcgcaa
     6421 cgcgcgcacg tcgcgcacca gatccgtgac cgcggtcatc cggcccgtgg acggctgctc
     6481 ttcttcccac acgatgaggc agtcctgcgt ccggcacgtc gcggccggaa acatgtgcaa
     6541 gcgcggagcc gccgacagcg cgacgccgtc gggctgcccg acccggccgt ccggcccgag
     6601 ggtggcgccg tagagcgcgt agcgatcgcc ggcgcgcttg tccacccagg cgatcacgga
     6661 ctcgcccgag gccacgagtc cgcccaccgc gggataccct tgttcgcccg gcgccgcaca
     6721 caccgtgatc ggctccggat cgagcacccg gccgtcgtcg tccacccggg cggtggcaat
     6781 gtcgttgtca ccccgccgcc catccatcca cgcgaccacg aacgcgcggc cgctccacgc
     6841 cacggcgggc cgcgtctggt cccccggtcc gcctcccacc gcgatcgggt ccgggtcgag
     6901 caccttgccg tccggcgtga tccgcgtcgc gacaatgtcc cagcccgagc ccggccgctc
     6961 ctgcatccac accgcgagga acacccgccc gctccacgcc acgcgcggat gccggcgatt
     7021 gccctcgccg gcgcccacct tcaacccgtc gggatcgacg atggtgccgt ccggtcgcac
     7081 gcgcgccccg aagatctccc aattgcgccg gctgcgcagg tcctgccaca ccgccaggtg
     7141 cgtgtccccg ccccacgcca cgtccgggaa caacgtgtcg gacggccccg cgcccaccgg
     7201 aaacccctcc ggcggggcca ccgccccgtc aggggccacc cacgcgccgt agatgtcgct
     7261 gggaccgcgg cgggtggact gccacaccac cagaaaccgc gacccgttcc acgacggaac
     7321 cggatcttcc tcctcgtcgg ccgctcggga cgaaatcaca atctccggtc ccacatgtgg
     7381 agcgccctcc cgcgcgacat ccacccgagc gggcgatccc gcgcacgcga cgaccagggc
     7441 cggcaacacg acgctccacc gcctcagccc tcgcccccac tcctcccgcc atcgcatgag
     7501 cggccgcggg aggctcgtga caagctcgtg aaccagacga ttgaccacac tcatcacccg
     7561 gttaccgagg caccaccgcg atgcccagca acccatccac cgcgccgctt tccagccacc
     7621 cggtgacccg gcgctccgcg gcgtcgatca ccgcgatgcg gccggtttca cggtgcgtga
     7681 cgtacacgcg agtcccgtcc agggccaccg cgactcccca aggcaccgcc cccaccggca
     7741 cgaccgccac ctcgcgatcc gtggccgtgg acacgatcga cagcgtgtcc gagccgctgt
     7801 tggcgacata cgcgatttgc ccgtcgggcg agagcgcgat gccggccggg ttttgcccga
     7861 ccgggacggt ggccaccacc acgtcgcgcg cggtgtcgat gaccgacacc gaacccggca
     7921 tctggttggt gacgtacgcg aaccctccgt ccgcgggcac cgcgatttca gtcgggaaca
     7981 tcccgaccgg aatcgttccg accacggcgt aggtggccat ggacaccacc gacacggtcc
     8041 ccgcaaaata gttgaccacg tagaccgcgg gccgtcgggg aatgcccgcg accgcgaccg
     8101 gccgggcgcc gacgcgcggt tgtgccaaca agcgaccggt cgtggtggag atgaccgaca
     8161 gcgtctgatc cgtcagattc gccacgaaca aggcccgtcc gtccggcgac acgtccacgg
     8221 cctgcggcgt ggcgcccgcc tccacccggt ccacgcgccg cgtgtccgcg tggaccgcgg
     8281 ccacggtgcg gccctgccct cccacgtaca cggttttgcc gtccggggac acggtcacgt
     8341 cccacggttt ggggatgtcc ccgatgcgcg cgaccgcggc ccggccgtcg ggatcgacca
     8401 aggcgacgct gtcgccgcca ggatccgcga cgtacaacac gggcccgcgc gcggcggcgc
     8461 acccgatcag gcccgccgcc atccagcacc cggcggcccg gaccacccac gacgtccagc
     8521 gctcctgcat catggtcgct cctgcgacga gtacgcggtc aaaaacgccg cgacctcgtc
     8581 ctcctcggcg gttgaaaggt tcgcgatcgg cgccgtccgg tgcacggtct gtctccaatc
     8641 gcggcggttc gggggcacca gccagaagct gtgcggcgcc agattgatct cggggtccgt
     8701 ccacccgtga cagccgttgc acttggcgaa cgcggcgggt ggaaccagcg gggacggggg
     8761 gcgcccggca tcgcggacct tgagcgacgt gataatcgcg tccgc
//
LOCUS       DBNGTT010000490         3368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1267933, whole genome shotgun sequence.
ACCESSION   DBNGTT010000490 DBNGTT010000000
VERSION     DBNGTT010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1267933"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1741)
                     /gene="gpmI"
                     /locus_tag="AC16BC_11250"
     CDS             complement(158..1741)
                     /gene="gpmI"
                     /locus_tag="AC16BC_11250"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR3242235.1"
                     /translation="MTTTVSARPRPTVLIILDGWGVRREREGNAIALAGAPFYHRLLR
                     EYASLELTSSGESVGLPDEQMGNSEVGHLNLGAGRVVYQDFSRINRSIADGSFAENPV
                     LRAAVDAAAKPGATLHLVGLLSDGGVHSHIDHVFALLAMAKRRGARRVAVHAFLDGRD
                     TPPQSAVGYLRALEARMASEGVGRLATVSGRYYGMDRDKRWDRVGKTYDAIVSGIGVE
                     AKEAVGAVEQSYRQGVTDEFVVPIVLCEGGRPVAPMRDGDSAIFFNFRADRARQLTAA
                     LTQDAFDGFARRHRVQWSQFVTMTRFDERLAVPAAFEPVRLTRILADVLSGLGLTQLR
                     IAETEKYAHVTYFFNGGVETVYPGEDRILIPSPREVATYDEKPAMSALEVTDTVTAKV
                     ASGSYDFILINFANPDMVGHTGKLDAAIEAVRVIDRCLERVITAVVARGGAVVLTADH
                     GNLEQMFDESGGPHTAHTTNPVPCILIDRRLRLDAPAKPRVASRGIFADVAPTLLALM
                     GIPQPPEMTGRSLVELQRQ"
     gene            complement(1738..3078)
                     /locus_tag="AC16BC_11255"
     CDS             complement(1738..3078)
                     /locus_tag="AC16BC_11255"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3242236.1"
                     /translation="MRFFVTFVILLLGALAYLIHLNPAPVVFRVTQTRSVELGLSVLL
                     LGAIAFGGMLVLSALGMRELRRWWANWRVGRQRQVSGKVQEHLALAHRAWFSGRAKEA
                     VSHLGRVLDINPNHVPALLLLGTIKNQAGERDDAIRFHRRARAADDQNIEVALALGQA
                     LEAADRADDAVQVYRDVLKTDEQNPAVTARLRDLYLKLERWEDAHGLQERILGAAKAP
                     ADAAVAKRILCGIKYELGRMWTAKGDRDRARRAFRGAVKLDQRFVPAYVGWGELIIQE
                     GRPRDAAALYEKAYQSVPDIILLHRLEDLYIDMGEPEQILRCYHEAVRKDPDSHALRF
                     YLGKLYYRLEMLEEAQDVLSGIDASDEHFPDLHRLLGNLAERQGNDAAAIDEFKRAMG
                     LKQRVIVPYYCPVCDYHSTKYSGRCPRCKTWNTLSALPIVVKQKTERVITRMPW"
     gene            complement(3087..>3368)
                     /gene="rsfS"
                     /locus_tag="AC16BC_11260"
     CDS             complement(3087..>3368)
                     /gene="rsfS"
                     /locus_tag="AC16BC_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3242237.1"
                     /translation="VKAIAEAVDEALGAQGVRLLHSEGVEHGRWVLMDYGDVVVHVFH
                     REAREFYRIERLWADAPVIELSPARPESRSTRLSPFRDRDRVVPPIEPG"
BASE COUNT          601 a         1166 c         1040 g          561 t
ORIGIN      
        1 gcgccaaggg acatctgata cacgaagacc gcggtgttga tcacgatcaa cgacacggtc
       61 acgatcggcg tgatggcggt cggattctcg tcgcggatcg ggatcatcgg aagcgcgcgc
      121 ggaaacgggc gagccgtccc gcgcgggacg atgctgacta ctggcgctgc aactcgacca
      181 acgaacgccc cgtcatctcg ggaggctggg gaatgcccat caacgccaac agcgtgggcg
      241 cgacatcggc aaagatcccg cgcgacgcca cccgcggctt ggcgggcgca tccagccgca
      301 accgccggtc gatcaggata cacggcacgg ggttggtggt gtgcgcggta tgcgggccgc
      361 ccgactcatc gaacatctgt tcgaggttcc cgtggtcggc ggtcagcacg acggcgccgc
      421 ctcgggccac gaccgcagta atcacccgtt ccaggcagcg atcgatgacg cgaaccgcct
      481 cgatcgccgc gtccagcttg ccggtgtgcc ccaccatgtc gggattggcg aaattgatga
      541 gaataaagtc atacgaaccg gacgcgacct tggccgtcac cgtgtcggtc acttccaacg
      601 ccgacatggc cggcttctcg tcgtacgtgg ccacctcgcg cggcgaaggg atgaggatgc
      661 ggtcttcccc cgggtagacc gtctccaccc cgccgttgaa gaaataggtc acgtgcgcgt
      721 atttttcggt ctccgcgatc cgcagctgcg tcaaccccag tccgctcagc acgtcggcca
      781 gaatgcgcgt gagccggacc ggttcgaacg cggcgggaac cgccagccgc tcgtcgaacc
      841 gtgtcatggt cacgaactgc gaccattgca cgcggtggcg gcgcgcaaag ccgtcgaacg
      901 cgtcctgggt caacgccgcg gtcaactgcc gcgcgcgatc cgcccgaaag ttgaaaaaaa
      961 tcgcgctgtc gccgtcgcgc atgggcgcca ccgggcgccc gccctcgcac agcacgatcg
     1021 gcacgacgaa ctcgtccgtg acgccctggc ggtaactttg ttcgaccgcg ccgaccgctt
     1081 ccttggcctc gacgccgatt cccgacacga tcgcgtcata cgtcttgccg acccggtccc
     1141 agcgtttgtc gcgatccatg ccgtagtacc gtccggacac ggtcgccagc cggcccacgc
     1201 cttccgacgc cattcgggct tccagcgccc gaagataccc gaccgcgctc tgcggcggcg
     1261 tgtcgcgacc gtcgagaaac gcgtgaaccg cgacccgccg ggcgccccgt cgcttggcca
     1321 tggccagcaa cgcgaacacg tgatcgatat ggctgtgcac cccgccgtcg gacaacaacc
     1381 ctaccagatg gagcgtggcg ccgggtttgg ccgccgcgtc caccgcggcg cggagcacgg
     1441 gattctccgc gaacgagccg tccgcgatgc tgcggttgat ccgcgaaaaa tcctgataca
     1501 ccacccggcc ggccccgaga ttgaggtggc cgacctcgga gttgcccatc tgctcgtcgg
     1561 gcagccccac cgattcgccg gacgacgtga gttcgagcga ggcgtattca cgcaacagcc
     1621 ggtggtagaa cggcgcgccg gcgagagcga tggcgttgcc ctcgcgttcg cgtcgaacgc
     1681 cccaaccgtc gaggataatg aggaccgtcg gacgcggccg cgccgaaacc gttgtcgtca
     1741 ccacggcatc cgggtgatga cccgctccgt tttctgtttg accacgatcg gcagcgcgga
     1801 cagcgtgttc caggtcttac accgcggaca ccgccccgag tacttcgtgc tgtggtaatc
     1861 gcagaccggg cagtagtagg gcacgatgac gcgctgcttc aatcccatgg cccgcttgaa
     1921 ctcgtcgatc gccgccgcgt cgttgccttg acgttccgcc agatttccga ggaggcggtg
     1981 caggtccggg aagtgctcgt cgctcgcatc gatcccggac aagacgtctt gcgcctcctc
     2041 cagcatttcg agacggtagt agagcttgcc caggtaaaac cgcagcgcgt ggctgtcggg
     2101 atctttccga accgcttcgt gataacaccg caggatctgt tcgggttcgc ccatatcgat
     2161 gtacaaatcc tccaggcgat ggaggagaat gatgtcgggc acgctctggt aggccttttc
     2221 atataaagcc gccgcgtccc ggggacgtcc ttcctgaatg atcaactcgc cccagcccac
     2281 gtacgcgggc acgaatcgct gatccaactt gaccgcgccg cgaaacgccc gccgcgcccg
     2341 atcccggtcg cccttcgccg tccacatccg cccgagctcg tacttgatcc cgcacaatat
     2401 ccgtttggcc acggccgcgt cggccggcgc cttggccgcg ccgaggattc gttcctgaag
     2461 cccgtgcgcg tcttcccatc gttccagttt gagatacaga tcgcgcaacc gtgccgtgac
     2521 cgccgggttt tgttcatcgg ttttcaacac gtcgcggtac acctgcacgg cgtcgtccgc
     2581 ccggtccgcg gcttcaagcg cctgtcccaa cgccagcgcc acttcgatgt tctggtcgtc
     2641 cgcggcccgg gcccgccgat gaaaccgaat ggcgtcgtca cgctctcccg cctggttctt
     2701 gatcgtcccc aacaacagga gcgcgggaac gtggttggga ttgatgtcca ggacccggcc
     2761 gagatgcgag acggcttcct tggcgcgtcc ggaaaaccat gcgcggtgcg cgagcgccag
     2821 gtgttcctgc accttcccgc tgacctggcg ctgacggccc acgcgccagt tcgcccacca
     2881 gcgtcggagc tcccgcatgc ccagcgcgga cagcaccaac atcccgccga acgcaatcgc
     2941 cccgagcaat agcacggaca gcccgagttc cactgagcgc gtctgggtga cacggaacac
     3001 gacgggcgcg gggttgagat gaatgaggta cgccagcgcg ccaagcagga ggatgacgaa
     3061 agtgacgaaa aatcgcatgt cagggcttat cccggctcaa tgggcggcac aacgcggtcg
     3121 cggtcgcgaa acggtgacag gcgcgtggac cttgattcgg ggcgagcggg ggacaactcg
     3181 atcacgggcg cgtccgccca caggcgttcg attcggtaga actcccgcgc ctcacggtga
     3241 aacacgtgga caaccacgtc cccgtaatcc atgagcaccc accggccgtg ttccaccccc
     3301 tcgctgtgca gcaagcgaac accttgggca ccgagcgcct cgtccacggc ctcggcaatc
     3361 gccttgac
//
LOCUS       DBNGTT010000491         1573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_372994, whole genome shotgun sequence.
ACCESSION   DBNGTT010000491 DBNGTT010000000
VERSION     DBNGTT010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_372994"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="AC16BC_11265"
     CDS             <1..1419
                     /locus_tag="AC16BC_11265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3242238.1"
                     /translation="YADGRLVGTQAIVRNMTERQEWEARLAKVTRHDHLTNLPNRAVF
                     MDRLDQALVRVRNRRSGRPLAVLMLDLDRFKLVNETFGHATGDRMLLAVAERLSGCLR
                     DGDTVARLGGDEFAVLLPEIARVSDTALVVDKIFAALKAPFLVDDHELFVSASVGISV
                     APDDAEESGLLLKNADAAMYRAKDQGRSTFQLYSPAMNVSQLERLSLESHLRHALERD
                     EFVVHYQPQIELRSGRIIGMEALVRWRHPEWGLVSPARFIPVAEETGLIIPIGERVLR
                     AACEQNRRWQEQGVAPFRMSVNLSARQFQQVDLKTLVALVLRESGLDPRWLELELTES
                     LLMSDADRTVSTLNALHAMGVGLALDDFGTGYSSLSYLKRYPIDTIKIDQSFVRHITT
                     EADDAAIAIAIIALANSLKLTVVAEGVETEEQQTFLAQQGCDAIQGYLISRPIPAEEI
                     TTWLRGRGARGGSGTAGLPRAA"
BASE COUNT          272 a          476 c          564 g          261 t
ORIGIN      
        1 tacgccgacg gccggttggt ggggacccag gcgatcgtcc gcaacatgac ggagcgccag
       61 gaatgggaag cgcgtctggc caaggtcacc cgccacgacc atctgaccaa cctgcccaac
      121 cgcgccgtgt tcatggatcg cctggatcag gcgttggtcc gcgtgagaaa ccggcggtcg
      181 gggcgtccct tggccgtcct catgctggac ttggaccggt tcaaactcgt gaacgaaacg
      241 ttcggccacg cgaccggcga tcgcatgttg ctggcggtgg ccgaacggtt gagcgggtgt
      301 ctgcgcgatg gggacacggt cgcacggctc ggcggcgacg agttcgcggt gttgttgccg
      361 gaaatcgcca gggtgagcga caccgcgctg gtcgtggaca agattttcgc cgcgctgaag
      421 gcgccgttcc tggtcgacga ccatgaactg ttcgtgagcg ccagcgtcgg gatcagcgtg
      481 gcgcccgacg acgcggaaga atccggtctg ctgctcaaga acgcggacgc ggccatgtac
      541 cgcgcgaagg atcaggggcg gagcacgttc caactctatt cgccggccat gaacgtgagc
      601 cagttggagc ggttatcgct cgagagccac ctccgacacg cgttggagcg ggacgagttc
      661 gtggtgcact accagccgca gatcgagttg cggtccggtc ggatcatcgg gatggaagcc
      721 ttggtgcggt ggcgtcaccc ggaatggggg ctggtctccc ccgcgcggtt tattccggtc
      781 gccgaggaga cggggttgat catcccgatc ggcgaacgcg tgctgcgcgc cgcgtgcgag
      841 cagaaccgac gctggcagga gcagggcgtc gcgccgtttc gcatgtcggt caacctgtcg
      901 gcgcgacagt ttcagcaggt cgatctcaag acgttggtgg ccctggtgct ccgcgagagc
      961 gggctcgacc ccaggtggct cgagttggaa ctgaccgaat cgttgctgat gtcggacgcg
     1021 gaccggaccg tctccacgct caacgcgctc cacgccatgg gcgtggggct cgcgctggat
     1081 gatttcggca ccgggtactc atcgctgagc tacctcaagc gatatccgat cgacacgatc
     1141 aaaatcgatc aatcctttgt tcggcacatt accaccgaag cggatgacgc cgcgatcgcc
     1201 atcgcgatca tcgcgctggc gaacagcctc aagctgaccg tggtcgccga aggggtcgag
     1261 acggaggagc agcagacgtt cttggcgcag caagggtgtg acgcgatcca gggctatctg
     1321 atcagccggc cgattccggc ggaggagatc acgacgtggc tgcggggacg gggagcccgc
     1381 gggggaagcg ggacggcggg gttgccccgc gcggcgtaac gcgaggcgtc gaccgagaca
     1441 acaacgaggg ccgcggtcag tccgaccgcg gccctcgcgc gttgatccga ccggcgttac
     1501 gccgggttgc gcagcgccgc gattcgggct tcgatcggcg ggtgggtcgc gaacaggtgg
     1561 agccatccgc gac
//
LOCUS       DBNGTT010000492         3860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3184384, whole genome shotgun sequence.
ACCESSION   DBNGTT010000492 DBNGTT010000000
VERSION     DBNGTT010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3184384"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..2625
                     /locus_tag="AC16BC_11270"
     CDS             364..2625
                     /locus_tag="AC16BC_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HIR3242239.1"
                     /translation="MPTRGGGDNTGGIRVPSVGYFPAATHQPRITIFGTKSAGTAIFM
                     DGFLRVARDSNTTWSTAVDLTIEGLNTFTFDAMDVNGSVSDPLTVSITRDTNPPLAPA
                     VSFPSSWPTNPVTISGTKEAGTFIRLNGRRISLLSDDTNWAYQVTLSPGPNTLSLTAV
                     DAAGNESGPVTGAVTLTASGCVAPPRLVAPLDGRSIAWGHAFSWTHVPVGTYVFELSA
                     SPAFENPLQHQAPVAGALQYAPVGLVPPNGVYYWRVGVQDACGTSYSPTRKVILGAMT
                     GDVTGDGFADIFVGAYTDARAGDGAGAAYLYRGGAVQDVTADAVFRGRQSGGTFGASV
                     AKVGDIDGDGYVDLLVGAPRADRDSAANDDTGAAYLYWGGPTPSASPALTFRGETDGS
                     IFGMSVAGVGDINGDGFPDIAIGAYQTPVTAECNGGTARLPIVGRVYVFFGGPRDQMD
                     NFPDVVLSGETTEIPNDRSSQCRSRDEFGLSVAGVGDVNGDGYDDIAVGARGYDTSLS
                     PPAGLDTGRAYVFYGGPWLVGVGADRADVVLTGLAENDQFGAAVAGSGDTNGDGLADL
                     LVGAHLSDLGGSDSGAAFWYFGESSGGMFGPVQLSGAVAGDSFGASMASAGDINGDGY
                     ADFMIGAYLVGATPNDIGAVSYFLGNSAGTVSPAGTISGETTPNTGDQFGLAVAGAGD
                     VDGNGFDDTVVGATEHDVCVIAPLPPGVCFNAGRAYVILGPTITNRGAAADPKDWLLT
                     GLTSDDRMGSSVN"
     gene            complement(2636..>3860)
                     /gene="murJ"
                     /locus_tag="AC16BC_11275"
     CDS             complement(2636..>3860)
                     /gene="murJ"
                     /locus_tag="AC16BC_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3242240.1"
                     /translation="PGFGDDPGKAALTADLTRIMFPYLLWISLAALAMGVLNSVRAFA
                     APALSPALFNLAIIATVFGLAPLLAEPVLAVAWGVFIGGLAQLAVQIPSLQRAGMGLG
                     WFWQPTHPGLAKMGALLIPTLVGLSVSQVNIFINTLLASYLAQGSVTYLYYAMRLVQF
                     PLGVFGVALSTALLPTLSTHAAKQDEAALRETLAFGLRLILFITIPAMIGLIALRTPI
                     IHVLFQHGRFTAADTAGTAVALLGYSVGLWAFAGVRVVVPVFYARQDTKTPVVVAAAA
                     VLVNVGLSLVLMGPLAHGGLALATAIASCVNFTVLLVVLRTRLGPFGGRRVVRSAAIA
                     LVASAPAAVIGWGVSGLSLWNVPGAWTEKVALISVAIAASAGAYALLHTLWKTDEATA
                     VWDLLKRRAARSRTT"
BASE COUNT          728 a         1176 c         1275 g          681 t
ORIGIN      
        1 atgcgctggt cgaggccacg gagaatatct tgaagttctt ccatatagag aagcctgcat
       61 catagtgtgc atgctatata tagcatgcta tagtgcatag catttgacag caacatagca
      121 tgagtaatat ttgtcgtcaa gaaaaaatat ttgtattaaa actatagcac tttattagta
      181 ccaacatagc aagtatccta ttcaagcagt gcgctgtatc gtttcgcggg gaacaggctc
      241 tcgaagaaat cgggggaata tggtggctcg taaacggttc tctctcctcg tggtgctacg
      301 cattcgccaa caagtcctgc gatgggcctg gctcatcgga ttctcggctt gtgcgctggt
      361 ggcatgccaa cccggggggg cggggacaat acgggtggaa tccgtgttcc gtccgtcgga
      421 tactttcctg cagccactca ccagccccgt atcaccatct tcggcaccaa gtccgctggc
      481 accgccattt ttatggacgg attcctgcgg gtcgcccgcg acagcaacac cacgtggtcc
      541 acggcggttg atctgactat tgaaggcctg aacacgttca ccttcgacgc gatggacgtg
      601 aatgggagtg ttagtgatcc gctaacggtc agcattacgc gcgatacaaa tcctccgctt
      661 gctcctgccg tgtccttccc gtcgtcgtgg ccgaccaacc ccgtgaccat ttccggcacc
      721 aaagaggccg gaaccttcat ccgcctcaat ggccgccgga tctcattgct ttcggacgat
      781 accaattggg cgtatcaggt gaccttgagt ccagggccca acacgctcag tctgaccgcg
      841 gttgacgcgg cggggaacga gagcggtccc gtcacagggg cagtcacgct caccgcgtcg
      901 ggttgtgtcg ccccaccgcg actcgtagcc ccgctcgacg gccgttcgat tgcgtggggc
      961 catgcgttct cgtggaccca cgttcccgtc ggcacctacg tatttgagct ctcggcatcc
     1021 ccggcgttcg agaatccact gcaacatcag gcaccggtgg cgggcgcgct gcaatatgct
     1081 ccggttggcc ttgtgccacc caacggggtg tactactggc gggtgggagt tcaggacgcg
     1141 tgtggcacgt cgtacagccc gacccgtaag gtcatcctcg gcgccatgac cggcgacgta
     1201 acaggcgatg ggtttgcgga catcttcgtc ggcgcctaca ccgacgctcg cgcgggtgac
     1261 ggtgccgggg ccgcgtatct ctataggggc ggagcagtcc aggacgtgac ggcagacgcg
     1321 gtgttccggg gtcgccagtc agggggcacg ttcggagcct cggtggccaa ggtcggcgat
     1381 atcgacgggg acggctacgt ggacctcctg gtgggcgcgc cccgagcgga ccgcgactcc
     1441 gcggccaacg acgataccgg agccgcgtat ctctattggg gcggtccgac gcccagtgca
     1501 agcccagcat tgacgttccg cggtgagacg gacggctcga tcttcgggat gtcggtggcc
     1561 ggggtcgggg acatcaacgg tgacggattt ccggatatcg ccatcggggc ataccagaca
     1621 ccggtgacgg ccgagtgtaa cgggggcacc gcgcggctgc cgatcgtcgg acgggtctat
     1681 gtgttcttcg gcgggccgcg cgatcagatg gacaatttcc cggatgtggt cctgtccggg
     1741 gagacgaccg agatcccgaa tgaccggtcg agccagtgcc ggtccaggga tgagttcggc
     1801 cttagcgtgg cgggcgtggg agacgtcaac ggcgacgggt atgacgacat cgcggtcgga
     1861 gcccgtggct acgataccag tctctctccg ccggccggac tggataccgg acgcgcctac
     1921 gtgttttacg gcggaccgtg gctcgtgggt gtgggcgccg accgtgccga cgtggtgttg
     1981 accggacttg ccgagaacga tcagttcggg gccgcggtcg ctggttccgg cgacaccaac
     2041 ggcgacgggt tggcggatct cctggtgggc gctcatttga gcgacttggg tggaagcgat
     2101 tccggggccg cgttctggta cttcggggag agcagcggcg ggatgtttgg gccggttcaa
     2161 ctcagcggag ccgtggccgg ggatagcttt ggggcgtcga tggcttccgc aggcgacatc
     2221 aacggggacg ggtatgcgga ctttatgatc ggggcctatc tcgtcggcgc cacccccaat
     2281 gacatcgggg ctgtttcgta ttttcttggc aacagcgctg gtacggtgtc gcctgccgga
     2341 accatttccg gggagacgac cccgaacacc ggcgatcaat ttggattggc cgtggccggc
     2401 gccggtgacg tggacggtaa cggattcgac gacacggtgg tgggtgcgac cgagcacgac
     2461 gtgtgcgtga tcgccccact ccccccgggc gtctgtttca acgcggggcg ggcctatgtg
     2521 atccttggcc cgacgattac aaaccgggga gcagctgccg acccaaagga ctggttgctc
     2581 accggcctca cgtccgatga ccggatgggc tcgtccgtca attgatcagc ccgcatcagg
     2641 tcgtccgcga ccgcgcggcg cggcgcttga ggaggtccca caccgcggtc gcctcgtcgg
     2701 tcttccatag ggtgtgcaac agggcgtagg ctccggccga cgccgcgatc gcgacgctga
     2761 tcagcgcgac cttctcggtc cacgcccccg gaacgttcca cagcgacaac ccgctcacgc
     2821 cccagccgat cacggcggcg ggcgcgctgg ccaccagggc gatcgcggcc gagcgaacca
     2881 cgcggcggcc gccgaatggg ccgaggcggg tccgtaatac caccaacagg accgtaaaat
     2941 tgacgcacga cgcgattgcc gtggcgagcg cgagaccgcc gtgggccaag gggcccatca
     3001 agaccagact gagaccgaca ttgaccagca cggctgcggc ggccaccacc accggcgtct
     3061 tggtgtcctg ccgcgcgtag aacaccggca ccaccacacg cacgccggca aacgcccaca
     3121 gacccaccga atagcccaac aaggcgacgg cggtccccgc ggtgtccgcg gcggtaaaac
     3181 gtccgtgctg gaataagacg tggataatcg gcgttcgcag tgcgatcaag ccgatcatgg
     3241 cggggatcgt gatgaacagg atcagtcgaa gcccgaaggc cagggtctcg cgtaaggccg
     3301 cttcgtcctg cttggccgcg tgcgtggaca aggtggggag cagcgcggtg gacaaggcta
     3361 cgccgaagac gcccagcgga aactgcacca ggcgcatggc gtaatacagg tacgtgacgc
     3421 tgccttgcgc aaggtaggat gccaggagcg tattgataaa aatgttgacc tgcgacaccg
     3481 acaaccccac gagcgtgggg atgagtaggg cccccatctt cgcaagaccc gggtgcgtgg
     3541 gctgccagaa ccagcccaat cccatgcccg cgcgctggag gctcgggatt tgaaccgcca
     3601 actgcgcgag gcctccgatg aacacgcccc acgccacggc cagaaccggc tcagcgagaa
     3661 gcggggccag accgaacacg gtggcgatga tcgccagatt aaacaaagcc ggagacaatg
     3721 cgggggccgc gaacgcacgg accgagttca gtacgcccat ggccaatgcg gcaaggctga
     3781 tccagagcaa gtacggaaac atgatgcggg tcaggtcggc ggtcaacgcg gccttgccgg
     3841 gatcgtcgcc gaaccccggg
//
LOCUS       DBNGTT010000493         4527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1397150, whole genome shotgun sequence.
ACCESSION   DBNGTT010000493 DBNGTT010000000
VERSION     DBNGTT010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1397150"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1004)
                     /locus_tag="AC16BC_11280"
     CDS             complement(69..1004)
                     /locus_tag="AC16BC_11280"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - L-threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR3242241.1"
                     /translation="MTDFRDTVTVFAPASTGNIGPGFDVLGMALAGLGDTVHARRIPE
                     PKVVILEIRGDGGKLPTNARKNTAGIAAAKVLKLLKVDAGVELVLEKNIPGNGLGSSA
                     ASAIAGGFAVNALFGSPLSKADLLLPCAAAEASVSGGYFLDNAAASLFGGVNVTNPET
                     REVISLGGLAGAIIVVATPRYQLLTARSRKVVPKRVPLASMIANCSRACAIVAAVAKQ
                     DADLFGRSIVDVVVEPARAPLIPGFAAVKRAALKAGALGCSISGAGSSVFAVTTDKAL
                     GESIGGAMQRAFERVRLECVVTVTTIDPTGARVVL"
     gene            complement(1114..1536)
                     /locus_tag="AC16BC_11285"
     CDS             complement(1114..1536)
                     /locus_tag="AC16BC_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242242.1"
                     /translation="MSPSSAVMESVKTWLAGRGVASELDEDGRLSFEAARGECLVEVD
                     PAGVVRCQFGIDLEELRDLIAGSATEDLGEDELVRAARFHLQTLAKPYAARLAAGQFV
                     EEYEVTQAYAVIGYVHPVDLSRPDTVLPLIERSLEILR"
     gene            complement(1533..2330)
                     /locus_tag="AC16BC_11290"
     CDS             complement(1533..2330)
                     /locus_tag="AC16BC_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3242243.1"
                     /translation="MPCYDPRVRDHPLNTLGNQAFARGDLAAALRYYQQALDAERRTG
                     SDRSLAVTLGNLANVYASMGRRDEAEGLYRQILEIERRLGDERQIALTLLHLGNLAAD
                     ALETSRARAHYLESRDLLSRLGETGALGVLYQNMGLLAREVGEYQEAVGAFESAIELM
                     RRADDRRGIAAVYTNLGRTYHLMGRLDEALACCSTSHAMAERAGDELAIASARYHLAG
                     ICEARGDRTAAIAHLRIVVDIDRRYGLPKYRENRARLAVLEREATPG"
     gene            2397..3029
                     /locus_tag="AC16BC_11295"
     CDS             2397..3029
                     /locus_tag="AC16BC_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242244.1"
                     /translation="MSRDLTIWVGVVAVGLAASVSEPRSAAAQDATWLTGPAEVGLAF
                     LTNIAIHESGHVLIAEGVGADKATLKFFGEDNGVYFLGLSTATGVPDDSLMTYRLGGE
                     IASSYTFELALRSFRQRPTVYNSALMFFSGTDFFWYTVFAFYIAPYEDPRYDPVGLRE
                     STGVSRGTIVAAAATQLAANAWRVFSGTDVVTPSFTYDRRSASLNLTVQF"
     gene            complement(3007..3819)
                     /gene="rsmA"
                     /locus_tag="AC16BC_11300"
     CDS             complement(3007..3819)
                     /gene="rsmA"
                     /locus_tag="AC16BC_11300"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR3242245.1"
                     /translation="MNTDERPRKSLGQCFLTDPRIAARIVSLGDVGHLDRVVEIGPGR
                     GILTRVLAERAAEVTAIELDPRWSAALREEFAGNARVRIEHADALAYPFEELASGFKV
                     IANLPYNVSTPLLFRLLSLRDRISLLVLMLQREVVDRLLARPGTKAYGALSVAVAYAA
                     NVRKGFPVAAGSFFPRPAVQSSVVVITPRATPAITVHDEALLMRVIRGAFSHRRKVLA
                     NALTDEGFEKPGVAAALERAEIDPARRGETLSLEEFGRLADAVAALRTERSG"
     gene            complement(4006..>4527)
                     /locus_tag="AC16BC_11305"
     CDS             complement(4006..>4527)
                     /locus_tag="AC16BC_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3242246.1"
                     /translation="QRTSQLEQRNQELFLLHAIASSLNKAQSLDDVLGELLNKVLGGV
                     EAHAVFIGLAEPGRRVSPLHGRPQFVAAQDDMAHLAESVVQQALTLGDLVVVPDTGAA
                     PEYQVFSRQAKSLIGIPLRAKDKTLGAIVLVYLKPRVPTPEEREFLSSIGHQAGVVIE
                     NARLFGLFKNLAG"
BASE COUNT          833 a         1512 c         1409 g          773 t
ORIGIN      
        1 tggaggaata actgctttgc acagataaga aaaagataac gagcactgtc ttcatgaggc
       61 acaacagctt acagaacgac gcgtgccccg gtgggatcaa ttgtcgtaac agtaaccaca
      121 cattccaggc gaacgcgctc aaatgcgcgt tgcatggcgc caccgatact ctcaccaagc
      181 gccttgtccg tggtcaccgc gaacacggac gatccggccc cggatatgga acaaccgagg
      241 gcgccggctt tgagcgcggc gcgtttcacg gcggcgaagc cggggatcag cggcgcgcgg
      301 gcgggctcca ccaccacgtc cacgatggaa cggccgaaca agtcggcgtc ttgtttggct
      361 acagctgcca cgatcgcaca tgcgcgcgag cagttcgcga tcatcgacgc gaggggcacg
      421 cgtttgggta ccactttgcg cgagcgcgcg gtcaggagtt gatagcgcgg cgtggccacc
      481 acgatgatgg cgcccgcgag cccgcccagg gagatcacct cgcgcgtttc gggattggtc
      541 acgttgacgc ccccgaacag cgaggccgcg gcattgtcca ggaagtagcc gccactgacc
      601 gaggcctcgg ccgcggcgca gggcaataag aggtcggctt tcgacagggg cgagccgaac
      661 aaggcgttga cggcgaatcc gcccgcaatg gcgctcgccg cgctgctgcc caggccgttg
      721 ccagggatgt tcttctccag gaccaattcg acgcccgcat cgactttgag cagcttgagg
      781 actttggccg cggcaatgcc cgcggtgttc ttgcgcgcgt tggtcgggag tttacccccg
      841 tcgccgcgaa tttcgaggat cacgaccttg ggctcgggga ttcgccgtgc gtgcacggtg
      901 tcgcccaagc cggccaatgc catgcccaac acatcgaacc ccgggccaat gttgccggtc
      961 gaagccgggg caaaaacggt gacggtgtcg cgaaagtcag tcatcgtgcg atttaggctc
     1021 tcgaatcctc attgccacct gtaacagcca ccccctccat tgccctcccc ccttgagggg
     1081 agggtggggg gtgcattgtc atccccgtgc ccgttaccgc agaatctcca acgaccgttc
     1141 gatcagcggc aacacggtgt ccggccggga cagatccacc gggtggacgt agccgatgac
     1201 cgcgtacgcc tgggtgactt catattcctc gacgaattgt ccggcagcca gacgcgccgc
     1261 gtagggtttg gccaatgtct gcagatgaaa ccgcgccgcg cgcaccagtt cgtcctcacc
     1321 caggtcttcg gtggcggacc ccgcgatcaa gtcacgcagt tcctccaagt cgatcccgaa
     1381 ctgacagcgc accacgccgg ccggatccac ctccaccagg cattcgccgc gcgccgcctc
     1441 aaaactcaac cggccgtcct cgtcgagttc ggaggctacg cctcgcccgg ccaaccaggt
     1501 tttgacggac tccatgaccg cgctcgacgg actcatcccg gcgtcgcctc gcgttccagc
     1561 accgccagcc gtgcgcggtt ctccctgtac ttgggaagtc cgtaccgccg atcaatgtcc
     1621 accacgatcc gcaagtgcgc gatggccgcg gtccgatcgc cgcgcgcctc gcaaatgcca
     1681 gctaggtgat accgagcgga cgcgatcgcc agttcatcgc ccgcgcgttc ggccatggcg
     1741 tgtgacgtgg aacagcacgc caacgcctca tcgagtcggc ccatgagatg gtatgtccgg
     1801 cccaggttgg tatacaccgc ggcgataccg cgccgatcat cggcccggcg catcaattcg
     1861 atcgcggact caaaggcccc gaccgcttct tgatactcgc cgacctcgcg cgcaagaagc
     1921 cccatgtttt gatacaggac gcccaacgcc ccggtctcgc cgagacggct caggagatcg
     1981 cgcgactcca gataatgggc gcgagctcgg gaggtctcca acgcgtcggc cgccagattg
     2041 cccaaatgca gcagggtcag cgcgatctgg cgttcatccc cgagtcgccg ttcgatctcc
     2101 aagatctgcc ggtacaaccc ttcggcctca tcgcgccgcc ccatcgaggc gtacacgttg
     2161 gcgagattgc cgagcgtcac ggccaacgag cgatcggagc cggtccggcg ctccgcgtcc
     2221 agcgcctgct ggtaatagcg cagcgccgcg gcgagatcgc ctcgggcaaa ggcctgattc
     2281 ccgagcgtgt tgaggggatg atcgcgcacg cgcggatcat aacatggcat cggcttcgcc
     2341 tccaaccgct ggccaaaagt ggacagcccg ccaaatcgcg ctatcttgtt catcgcatgt
     2401 cgcgggacct gacgatctgg gtcggtgtgg tggccgtggg cctcgcagcc tcggtctcgg
     2461 agccgcggtc cgccgcggct caggacgcca cgtggctgac ggggccggct gaagtcggtc
     2521 tcgcgttcct aaccaacatc gccattcacg aatccgggca cgtcttgatc gcggaaggag
     2581 tgggcgcgga caaagccacc ctcaagttct tcggcgaaga caacggcgtc tacttcctcg
     2641 ggttgagcac cgcgacgggc gttcctgacg actcgctgat gacttatcgc ctgggcgggg
     2701 aaatcgcctc cagctacacc ttcgagctgg cgttgcgaag cttccggcag cgtccgaccg
     2761 tgtacaacag cgcgttaatg ttcttcagcg gaaccgactt tttctggtac accgtgttcg
     2821 ccttctatat cgcgccctac gaagacccgc gctacgaccc ggtcgggctc cgggagtcga
     2881 ccggggtttc gcgcggcacg atcgtggccg cggccgcgac ccaactcgcg gccaacgcgt
     2941 ggcgcgtctt ctcgggcacc gatgtcgtga cgccgtcctt cacctacgac cgccggtccg
     3001 ccagccttaa cctgaccgtt cagttctaag cgccgccacc gcgtcggcca gacgaccgaa
     3061 ctcttccaga ctcaacgtct cgcctcgtcg cgccgggtcg atctccgctc gttccagtgc
     3121 cgcggcgacc ccaggctttt caaagccttc atcggtcagc gcgttcgcca acaccttgcg
     3181 ccggtgcgag aacgcgccgc ggatcacgcg catcagcaac gcttcgtcgt gcaccgtgat
     3241 ggccggcgtt gcgcgcggcg tgatgaccac caccgaggac tgcacggcgg gacgagggaa
     3301 aaagctgccc gccgccaccg gaaacccttt gcggacgttc gcggcgtatg ccacggccac
     3361 ggagagcgcg ccgtatgcct tggtgccggg acgggccagc aggcggtcca ccacctcgcg
     3421 ttgcagcatg aggaccagca gcgagatccg atcgcggaga ctcagcaggc gaaaaagaag
     3481 cggcgtcgag acgttatagg gcaaattggc gatgaccttg aatcccgatg cgagttcttc
     3541 gaacgggtac gccaatgcgt cggcgtgttc gatgcgcacc ctggcgtttc cggcgaactc
     3601 ctccctgagc gcggcgctcc accgcgggtc gagttcgatc gcggtcacct cggccgcgcg
     3661 ctcggccaac acgcgcgtca agataccgcg gccgggaccg atctccacca cgcgatccaa
     3721 atggccgacg tccccaagcg agacgattcg cgccgcgatc cgcggatcgg tcagaaaaca
     3781 ttgcccgagc gacttgcggg ggcgctcatc agtgttcatc aacatggcgt tgtcggaaac
     3841 gccgatttct tcgtgttccg cgtagtagga tctgcaatgg agcctgttca ggaacgcgcc
     3901 agatgcaagg cggagggaga gccttgcgcg cggagcgtac tgggtcgtac gtgagcacgc
     3961 aaggccgact gacaacgccg cagatggccg ttcatggaca ggctcctacc cggcaagatt
     4021 cttgaacaga ccaaacaggc gggcgttctc gatcaccacg cccgcttgat ggccgatcga
     4081 ggacaggaac tcccgctcct ccggcgtggg aacgcggggc ttcagataga ccaggacgat
     4141 ggcgcccagc gtcttgtcct tggcccgaag cggaatgccg atcagcgact tggcttgacg
     4201 cgaaaacacc tggtattcgg gggcggcgcc ggtgtcgggc accaccacca ggtctccgag
     4261 cgtgagggct tgttggacga ccgactccgc caagtgcgcc atatcgtcct gcgccgccac
     4321 gaattgcggg cggccgtgaa ggggcgagac gcggcggccc ggctcggcaa gaccgataaa
     4381 caccgcgtgg gcctcgaccc cgccgagaac cttgttgagc aattcgccga gcacatcgtc
     4441 caacgactgg gctttgttga gcgatgaggc gatcgcgtgc agcaaaaaca gttcctggtt
     4501 gcgctgttca agctggctcg tgcgctg
//
LOCUS       DBNGTT010000494         2339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2673840, whole genome shotgun sequence.
ACCESSION   DBNGTT010000494 DBNGTT010000000
VERSION     DBNGTT010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2673840"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="AC16BC_11310"
     CDS             <1..395
                     /locus_tag="AC16BC_11310"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR3242247.1"
                     /translation="ELGLTDVDFEDRRFHVHFPAGAIPKDGPSAGITITTALASAATG
                     RRVRQDVAMTGEVTLRGKVLPIGGLKAKALAAHRAGIKTIIVPKGNARDLDTIPEELR
                     KDLDVVTVDTIEEVLDRALEPAPRSGGV"
     gene            431..844
                     /locus_tag="AC16BC_11315"
     CDS             431..844
                     /locus_tag="AC16BC_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR3242248.1"
                     /translation="MTLLETRILRGLKQRASRLKAETFALYLAARDPRTPWYAKLLVA
                     GIVAYAISPIDLIPDFVPVLGYLDDLILIPMGIAFAITLIPHSVLTECRARAQDTARD
                     GRPVSRVAGTVIVLIWIGLAALCVMWAYEAFIMPP"
     gene            929..1687
                     /locus_tag="AC16BC_11320"
     CDS             929..1687
                     /locus_tag="AC16BC_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242249.1"
                     /translation="MTTRSKVLSLTAIALALIMAGGLWWLYHSLGPLVATTIRTFGPE
                     ITGVAVRIDDVTIEPFKGTAALHGLVVGNPKEFKADHALSLGEFSMTLKLRSLLGNVI
                     VIKQIVIAKPEVTYEIGADGSNLAAIQRNVDRYVGSSGAVDRPAKEPTARSDNGGKKL
                     VIQDLVIKDATAHVSAAFLQGKTLSVPLPDLHLENIGQESNGATAGEAVKQVFGAITK
                     SVTVAVAALNLSGATESLQKGAGSVGGFIKGLLK"
     gene            complement(1789..2157)
                     /locus_tag="AC16BC_11325"
     CDS             complement(1789..2157)
                     /locus_tag="AC16BC_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3242250.1"
                     /translation="MKAGTIALFAALVVSGVFQLTAVQPASAGEQTVMIETQSFAPQV
                     MTVHVGDRVTWSNRDHTEHFLTSSGPMTRSVATQVGDLEFHQRMIPGSDYSHSFKSPG
                     VYGYFCAIHMGMWGTIVVEP"
BASE COUNT          425 a          773 c          730 g          411 t
ORIGIN      
        1 acgagctcgg cctgaccgac gtcgacttcg aggaccgccg gttccacgtc cacttccccg
       61 ccggcgccat ccccaaggac ggcccgtcgg ccggcatcac catcaccacc gcgctcgcct
      121 ccgcggccac gggccgccgc gtgcgccagg acgtggccat gaccggcgag gtcaccctgc
      181 gcggcaaggt gctgccgatc ggcggcctca aagccaaagc cctcgccgcc caccgcgccg
      241 gcatcaagac catcatcgtg cccaagggca acgcccggga tctcgacacc atccccgaag
      301 aactccgcaa ggacttggac gtggtcacag tggacaccat cgaagaagtc ctggaccgcg
      361 ccctggagcc cgcgccgcgc tcagggggcg tttgactcgt cgaagatgtc ttcttggtaa
      421 tgcgacgtca ttgactttgt tggaaaccag gattcttcgt ggactgaaac aacgtgccag
      481 tcgtctcaag gccgagacct tcgcgctcta ccttgccgcg cgtgacccca gaacgccgtg
      541 gtacgcaaag ctgctcgttg ccgggatcgt ggcgtatgca attagcccca ttgatctgat
      601 cccggacttt gtccccgtgc ttgggtatct ggatgatctc atcctgatcc ctatggggat
      661 cgcctttgcc atcacgctga tccctcattc ggtcttgacc gagtgccgag cgcgggcgca
      721 agatacggcg cgggacggaa gacccgtgag ccgggttgcg ggcacggtga ttgtcctcat
      781 ctggatcggg ttggcggcgc tctgcgtcat gtgggcctat gaagcgttca tcatgccacc
      841 gtgagcacgc ctatgtcgta cgacctgtcg accataacgg aggtgagccc ggactccggt
      901 ggttcgcatt tcaacaagga ggttccccgt gacgacccgc tcgaaagtgc tctctctcac
      961 ggccatcgcg ctggcgctca tcatggccgg cggtctgtgg tggctgtacc actcgctcgg
     1021 accgttggtg gcgaccacga tccgcacgtt cggccccgaa atcaccggcg tggccgttcg
     1081 catcgacgac gtgacgatcg agccgttcaa gggcacggcc gcgctgcacg gtctcgtggt
     1141 cgggaatccc aaggagttca aggcggacca cgcgctgtcg ttgggcgaat tcagtatgac
     1201 gctcaagctc cggtctctgc tcggcaatgt catcgtcatc aaacagatcg tgatcgcgaa
     1261 gcccgaggtg acgtacgaga tcggcgcgga cggcagcaat ctggccgcca tccagcgcaa
     1321 cgttgatcgc tacgtcggaa gctccggcgc cgtggaccgg cccgccaagg aaccgaccgc
     1381 tcgaagcgac aacggcggga agaaactcgt gatccaggac ctcgtgatca aggacgccac
     1441 ggcgcatgtc agcgccgcgt tcctgcaggg caagacactc tcggtccccc tgcccgacct
     1501 ccatctcgag aacatcggtc aggaatcgaa cggcgccacc gccggggagg cggtcaaaca
     1561 agtgttcggc gcgatcacca agagcgtgac cgtcgcggtg gccgcgctca atttgagcgg
     1621 cgcgacggaa tcccttcaaa aaggcgcggg gtcggtcggg ggattcatca aggggttatt
     1681 gaagtaagga cggtcgaacc gcgttccgcg tctcccctgg acgctgaaca cgcggggccg
     1741 ccgcgccaga cggcgcggcg gccccgcatc aatcgaatcg ttgcgggctc agggttcgac
     1801 gacgatggtg ccccacattc ccatgtggat cgcgcaaaag taaccgtaca cgccgggcga
     1861 cttgaacgag tgcgaatagt cgctgcccgg gatcatgcgc tggtgaaact ccaagtcgcc
     1921 cacctgggtc gccaccgacc gggtcatggg gcccgaggac gtcagaaaat gttcggtgtg
     1981 gtcgcggttg ctccaagtca cgcggtcgcc gacgtggacc gtcatcacct gcggggcgaa
     2041 cgattgcgtc tcgatcatga ccgtctgctc gcccgcggac gcgggttgaa cggctgtcag
     2101 ttggaacacc cccgacacga cgagcgcggc aaacagtgcg atggtgcctg ctttcatagc
     2161 ggtcctccgg ttaggctgag ggcgccgact tcgcgccgtc cgatccgccc cgaaatcccc
     2221 gtcgtcccgg gtccggggcg cctagtcgaa gaaccaccgc accggaatcg ttattccgat
     2281 ccgggacttt ccagcaccgc gcctcgaccc cggtgtccgt tttggtgcaa cgcgggtca
//
LOCUS       DBNGTT010000495         5617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_758885, whole genome shotgun sequence.
ACCESSION   DBNGTT010000495 DBNGTT010000000
VERSION     DBNGTT010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_758885"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="AC16BC_11330"
     CDS             <1..1201
                     /locus_tag="AC16BC_11330"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3242251.1"
                     /translation="FPGYGFLSENPEFGQACIDAGLIFVGPSPDVIARMGDKLATKAV
                     LEGAGVPVIPGLKQVTSAKQIADFGNKVGWPIIIKAVAGGGGRGMVRVDGPEQAAKAV
                     EQAVSIAGKLFASAGVYAEKYVRNARHIEFQFLGDRFGHVIHLGERECSIQRRHQKLV
                     EEAPSSLLTEELRREMGAVAVKAMKEIGYVTAGTLEFLVDGDMRYYAIEVNPRIQVEH
                     TVTEMIAGLDLIRLMIRTAAGQALTVRQEDIVIRSHAIQCRVNAEDPKNQFAPSYGKV
                     TYLRQVSGPFVRADTGIYQGWEIPPYYDSLITKICSLAKDRATAIERMWRALCEYELW
                     GVKTTIPLLKKIMTHPEFIAGRFDTDFIDRHLAELLDYEEEEDELLRIARFVAEITAI
                     GKNPYCR"
     gene            1212..3293
                     /locus_tag="AC16BC_11335"
     CDS             1212..3293
                     /locus_tag="AC16BC_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3242252.1"
                     /translation="MKPANVHPSLSPKENLAILRRDPRVYFTNTGPRDTGQSDFKNRH
                     TLHDLIKLAPMYNQSGYFSIENHGGARFHQNLLQNKIDPFEEAALWKQRMPDVMTQTL
                     VRSTNLWGYRMYPRNVAALSVKAFLPYVDVWRCFDFLNYVPNMIPIAEEVMKGGKIFE
                     PAISFSVSDDCTNAYYLKVTRESLAITGGAKEIILCIKDMAGVGSPKRIASLVDALLQ
                     KYPSLVLHYHRHTTDGLAVPALVAAAKAGVKILDVTDDPFTRYYGHAPVRSVNALLQE
                     QGIQTRLDLPAVDESATVVADFIGHYHEFESQFRGFSYKVTEHRMPGGAFPSSFEQAG
                     KGDFLHLMPHILRGMSYGNKFIKYFDVTPGSQITWTTWVGIVQFHYKEGGYKAVESLL
                     GLCERFIAVRQRLEKLSAEERERLLGLYAHATDDLKSLLLGRYGPLPFGWPADWVYQS
                     VFGDAWREKIKKERIDASPLAKKKPENIPAIQRELEGKLGRSATPSELVLYLQHPGAA
                     VDFLAFRRKFGDTSLLPTPVWFDGLKGVGNEVRFEHHGKPTTIKLVSIGAEVNGMRHM
                     VLAVNNTMHVFPVDMPSSKERHKVVARMADPAIKGEIGSPMMGNVWRIGDKERTLKVG
                     DIVREGQEIMNLEAMKIETAILSPMHGVIKEIPVRLNEAVVDKQLLMVLGEVPWTQTK
                     RKAGSPKRAKK"
     gene            3313..4269
                     /locus_tag="AC16BC_11340"
     CDS             3313..4269
                     /locus_tag="AC16BC_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HIR3242253.1"
                     /translation="MTDSSSDYAELTDDALLGLLFTEADRLPRAAADEIIARGPQLVH
                     PLTWLAMDPSNWQADFPAGWAPIHATYLLGAISGEDALMGLLTAAEHAESVDCDWVLS
                     ALPAIFSRMGARARPHLSERVRDVTREPLFRAAMAECLAATVTREPSGADAVFAELGA
                     VFADATDETLKALVGGVLLDFQRGEYRDALLAHAREQAVGAEGDDAELIAYTRVDVDR
                     AFAPDATPVLDAYTDDWLAFYSPEEIAARQARWDKEDAEADDADVELGEEEWEPEFIP
                     QVVRLHPKIGRNDPCRCGSGKKYKQCCLERDATFEDLNLIGP"
     gene            4487..5443
                     /locus_tag="AC16BC_11345"
     CDS             4487..5443
                     /locus_tag="AC16BC_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3242254.1"
                     /translation="MKRILVTGATGQIGSELVPELRQRYGASNVVAGVHTRRAAPDVV
                     ESGPWRILDIRDRAAIDAAVVDHRIDSIMHLASLLSAVAEQRPQEAWDVNVNGLINVL
                     DVARERGCAVFFPSSIAAFGPLAPAEQTPQDAIQRPTTVYGITKVTGELLCDYYHGRY
                     GVDTRGLRYPGLVSSKTLPGGGTTDYAVHMFHAALKDRRYECFLQPDTRLDLMYMPDA
                     IHATIQLMEADGSRLRHRNAYNVTAMSVSPRQLADAITRRLPDFTVSYVIDPARQAIA
                     DSWPRHLDDRAAREEWGWRPRFDLYGMVGDMLDTLAARRAPS"
BASE COUNT         1019 a         1799 c         1909 g          890 t
ORIGIN      
        1 gtttcccggg tacgggtttt tgtccgagaa tccggagttc gggcaggcct gcatcgacgc
       61 cgggctgatc ttcgtggggc cgagcccgga cgtgatcgcg cgcatgggcg acaaactcgc
      121 gaccaaggcc gtgctggagg gcgcgggcgt gccggtgatc ccggggctca aacaggtcac
      181 gtcggccaag cagatcgcgg acttcggcaa taaagtgggc tggcccatca tcatcaaggc
      241 ggtggcgggc gggggcggcc gcggcatggt gcgcgtggac gggcccgagc aggcggccaa
      301 ggccgttgag caggccgtct ccatcgcggg caagctcttc gcgtccgcgg gcgtgtacgc
      361 ggaaaaatac gtccgcaacg cgcggcacat cgagttccaa ttcctgggcg accggttcgg
      421 ccacgtgatt cacctgggcg aacgcgagtg ctcgattcaa cgccggcacc agaaactggt
      481 ggaggaggcg ccgtcctcgc tgttgaccga ggaactgcgg cgggagatgg gcgcggtcgc
      541 ggtcaaggcc atgaaggaga tcggctacgt caccgcgggc acgctggagt tcctggtgga
      601 cggggacatg cggtactacg ccatcgaggt caacccgcgc atccaggtcg agcacaccgt
      661 gaccgagatg atcgcggggc tcgacttgat ccggctcatg atccgcaccg cggccgggca
      721 ggcgctcacg gtcaggcagg aggacatcgt gatccgcagc cacgcgatcc agtgccgcgt
      781 gaacgcggag gaccccaaga accagttcgc gccctcgtac gggaaggtca cgtacctgcg
      841 ccaggtcagc ggcccgttcg tgcgcgcgga caccgggatc tatcagggct gggagatccc
      901 gccgtattac gactcgctga tcaccaagat ctgctcgctg gccaaggacc gggcgaccgc
      961 gatcgaacgc atgtggcgcg cgctctgcga gtacgagctg tggggcgtga agaccacgat
     1021 cccgctgctc aagaaaatca tgacgcaccc ggaattcatc gccgggcgct tcgacacgga
     1081 tttcatcgac cggcacctcg cggaactgct cgactacgag gaggaggaag acgaactcct
     1141 gcgcatcgcc aggttcgtgg cggagatcac cgcgatcgga aagaacccgt attgccgatg
     1201 agcgcgaccc cgtgaaaccc gcgaacgtcc accccagttt gagccccaag gagaacctcg
     1261 cgatcctgcg ccgcgacccg cgcgtgtatt tcaccaacac cgggccgcgc gacacgggac
     1321 agagcgactt caagaaccgc cacacgctcc acgatctgat caagctcgcg cccatgtaca
     1381 accaaagcgg gtacttttcg atcgagaacc acgggggcgc gcggtttcac cagaacctgc
     1441 tgcagaacaa gatcgacccg ttcgaggaag ccgcgctctg gaaacagcgg atgccggacg
     1501 tgatgaccca gaccctggtc cgctcgacca acctctgggg ctaccggatg tacccgcgca
     1561 acgtggcggc gctgtcggtc aaggcgttcc tcccgtacgt ggacgtgtgg cggtgcttcg
     1621 actttctcaa ctacgtgccc aacatgatcc cgatcgcgga agaggtcatg aagggcggga
     1681 agatcttcga gcccgcgatc tcgttctcgg tgtccgacga ttgcaccaac gcctactatc
     1741 tcaaagtcac cagggaaagc ctggcgatca cgggcggggc gaaagagatc atcctgtgca
     1801 tcaaggacat ggcgggggtg ggctcgccca aacgcatcgc ctcgctggtc gacgccctgc
     1861 tgcagaaata cccgtcgctg gtgctccact atcatcgcca caccaccgac ggcctggccg
     1921 tgcccgcatt ggtggccgcg gccaaggccg gggtcaagat cctggacgtg accgacgatc
     1981 cgttcacgcg ctattacggc cacgcgccgg tgcggtcggt caacgcgctg ttgcaggagc
     2041 aggggatcca gactcgtctc gacctgcccg cggtggacga gtccgcgacc gtggtcgcgg
     2101 acttcatcgg ccactatcac gaattcgagt cccagttccg cgggttctcg tacaaggtca
     2161 ccgaacaccg gatgccgggc ggcgcgttcc ccagctcgtt cgagcaggcg ggcaagggcg
     2221 attttctgca tctcatgccg cacatcctcc gcgggatgag ctacgggaac aagttcatca
     2281 agtatttcga cgtcaccccg ggctcgcaga tcacgtggac cacgtgggtg ggcatcgtgc
     2341 agttccatta caaggaaggc gggtacaagg cggtcgaatc cctgctggga ctctgcgagc
     2401 ggttcatcgc ggtccgccag aggctggaga agttgagcgc ggaggagcgc gaacgtttgt
     2461 tgggcttgta cgcgcacgcc accgacgacc tcaagagcct gttgctgggc cggtacggcc
     2521 cgttgccgtt cggctggccc gcggactggg tctaccaatc ggtgttcggc gacgcgtggc
     2581 gcgagaagat caagaaggag cggatcgacg cgtcgccctt ggccaagaaa aagcccgaaa
     2641 acatcccggc catccagcgg gagttggagg ggaagctggg ccggagcgcc acgccgtccg
     2701 aattggtctt gtacctgcag cacccgggcg cggccgtgga ttttctcgcg tttcgccgca
     2761 agttcgggga cacctcgctg ctgcccacgc cggtgtggtt cgacggattg aagggcgtgg
     2821 gcaacgaagt gcgcttcgaa caccacggca agcccaccac catcaagctg gtgtcgatcg
     2881 gcgccgaagt gaacggcatg cggcacatgg tgctggccgt caacaacacc atgcacgtgt
     2941 tcccggtgga catgccatcg tccaaggagc gccacaaggt cgtcgcgcgc atggcggacc
     3001 ccgccatcaa gggcgagatc ggctcgccca tgatgggcaa cgtctggcgg atcggggaca
     3061 aggaacgcac gctcaaggtc ggagacatcg tgcgggaagg ccaggagatc atgaatctcg
     3121 aagccatgaa gatcgagacc gcgatcctgt cgcccatgca cggcgtgatc aaggagatcc
     3181 cggtcaggct caacgaggcg gtggtggaca aacagttgct catggtgctg ggcgaagtcc
     3241 cgtggacgca gaccaaacgg aaagccgggt cgcccaagcg cgcgaagaaa taggcgcagg
     3301 gccgcttcga ccgtgaccga ctcctcttcc gactacgccg aactgaccga cgacgcgctg
     3361 ctcgggttgc tcttcaccga ggcggaccgc ctgccgcgcg ccgcggcgga tgagatcatc
     3421 gcccgcggcc cgcaactggt ccatccgctc acgtggctgg ccatggaccc gtccaactgg
     3481 caggcggact ttccggccgg gtgggcgccg atccacgcca cgtacctgtt gggcgcgatc
     3541 agcggggaag acgcgctgat ggggttgctc accgcggcgg agcacgccga gtccgtggac
     3601 tgcgactggg tgctgagcgc gctgcccgcg atcttcagcc gcatgggcgc gcgcgccagg
     3661 ccgcacctgt ccgagcgggt ccgcgacgtg acgcgcgagc cgctctttcg cgcggccatg
     3721 gccgagtgtc tggccgccac cgtgacgcgc gagccctcgg gcgcggacgc ggtgttcgcg
     3781 gaacttggcg cggtgttcgc ggacgcgacg gacgagacgc tcaaggccct ggtgggcggc
     3841 gtgctactgg atttccaacg cggggagtat cgcgacgcgc tgctggcgca cgcgagggaa
     3901 caggcggtcg gggcggaggg cgacgacgcg gagctgatcg cgtacacccg agtggacgtt
     3961 gaccgcgcgt tcgcgccgga cgcgactcca gtgctcgacg cgtacacgga cgattggttg
     4021 gccttttact ccccggagga aatcgccgcg cgccaggccc ggtgggacaa ggaagacgcc
     4081 gaagccgatg acgcggacgt ggaactgggg gaggaggaat gggagcccga gttcattccc
     4141 caggtcgtcc ggctccatcc caagatcggt cgcaacgacc cgtgccgctg cgggagcggc
     4201 aagaagtaca agcagtgttg tctggaacgc gacgcgacgt tcgaagacct gaatctgatc
     4261 ggcccctgac ggcttgttct ccgattagcc ggtgagaacg gtgtacggcg ttgttggttg
     4321 cgaacgacgg acaacgtaca ccccgtttta tgttttgccg caaagcggct aagaattcac
     4381 cgagcagtcg gcgcatggtc actgccacct cacgaagggt caaatccccc catcccccct
     4441 ttgacaaagg ggggcaaggg gggattttct aatcgggcac gtctgcatga agcgcatcct
     4501 ggtcaccggc gccaccggac agatcggctc cgaactggtg cccgagctgc ggcaacggta
     4561 cggggcctcg aacgtggtgg ccggcgtgca cacgcgccgc gccgccccgg acgtggtgga
     4621 gtccggtccg tggcggatcc tggacattcg cgatcgcgcg gccatcgacg ccgcggtggt
     4681 cgaccaccgg atcgactcga tcatgcacct ggcctccctg ctctccgcgg tcgcggaaca
     4741 gcgcccgcag gaggcctggg acgtcaacgt caacgggttg atcaacgtgc tggacgtggc
     4801 gcgcgagcgc ggctgcgccg tgttcttccc cagctccatc gccgcgttcg gcccgctggc
     4861 gcccgcggag cagacgccgc aggacgcgat ccagcgtccc accacggtct acggcatcac
     4921 caaggtcacc ggggaattgc tctgcgacta ctatcacggc cgctacgggg tggacacgcg
     4981 gggtctgcgc tacccgggac tggtgtcctc caagaccttg cccggcgggg gcaccaccga
     5041 ctacgccgtg cacatgtttc acgccgcgct caaggaccgg cgctacgagt gtttcctcca
     5101 gcccgacacg cggctggatc tgatgtacat gcccgatgcg attcacgcga ccatccagct
     5161 catggaggcg gacggcagcc ggttgcgcca ccgcaacgcc tacaacgtca cggccatgag
     5221 cgtgtcgccc cgacagctcg cggacgcgat cacgcggcgc ctcccggatt tcacggtcag
     5281 ctacgtgatc gatccggcgc ggcaggccat cgcggactcg tggccccggc atctggacga
     5341 ccgggcggcg cgcgaggaat ggggctggcg gccgcgcttc gatctgtacg gcatggtcgg
     5401 ggacatgctg gacacgctcg cggctcgccg cgcgccgtcg tgagggattg ccgtgtcgct
     5461 gaacaagctt gagccgttgc tcacgcgcgc attggccgac cttgacagtg cgggccggcg
     5521 caaggggcgg gagcgcgtga tcgtgggcgt gacgccggct cacggcgcgt tcgggccccg
     5581 gtatctcctc gaaggatacg gggagcggga gttcctg
//
LOCUS       DBNGTT010000496         3927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_247968, whole genome shotgun sequence.
ACCESSION   DBNGTT010000496 DBNGTT010000000
VERSION     DBNGTT010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_247968"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="AC16BC_11350"
     CDS             complement(<1..562)
                     /locus_tag="AC16BC_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PulJ/GspJ family protein"
                     /protein_id="HIR3242255.1"
                     /translation="MTAIDVKRTTFRVDERGMSLAELLVATAVGLILAGLCGHALTTF
                     YAAYGTASDGIDREQQAHLALALICAELDTIVAAPRSTGCPSPGIQVVSGRAEFSANP
                     YDRETFLRDPAPQGTNQLALDTGATFEVGDTVLLLDIGTVWDPADDASQCVKIASIDG
                     NMLSVDGALVRSFPSRSPATLVNRLEY"
     gene            complement(559..939)
                     /locus_tag="AC16BC_11355"
     CDS             complement(559..939)
                     /locus_tag="AC16BC_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR3242256.1"
                     /translation="MIETLIALFLLTVGLTAVATGFTEGQRIVAEAQRRERAIGAARE
                     KLMERLALGYEHATDPVAPGEQELDGSLFGGDEDGGVIRRWYVEPEQPDHGLTRVSVV
                     TQWTRRGGRQTYRISGLLVRGRTP"
     gene            complement(975..1466)
                     /locus_tag="AC16BC_11360"
     CDS             complement(975..1466)
                     /locus_tag="AC16BC_11360"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR3242257.1"
                     /translation="MVRSNRTPSGYTLIEILTVLGIAAIVATIAWPATEDLIERAELS
                     SAARTMVGDIRLVQREARVSGRTLTLIVDPGSGSYAVSRPDTHTQTRRLAPTLRFGLP
                     DNPASDGVTFRDNTVFIAPRPGPQSSIGSISITTRRGAARRVTVSLTGHTAITVWDGR
                     QWN"
     gene            complement(1678..3099)
                     /locus_tag="AC16BC_11365"
     CDS             complement(1678..3099)
                     /locus_tag="AC16BC_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3242258.1"
                     /translation="MLSILIAEDNEGMAAMLTTALSGHGYDVIWARDGREAIQRLRDR
                     KIDLVVTDLKMPYKTGLDILQVAKEQNSLLPVVLMTAHGTIETAVGAVKDGAYDFLTK
                     PFDPDHLLLVIERALEKQRLVAENLLLKEDASAHAGLPRIIGKHPPVLAIVDQIQRVA
                     PSRTTVLLLGESGTGKELFARALHGLSPRREGPFVAINCAAIPKELLESELFGHERGA
                     FTGATDRRIGKFELADHGTIFLDEIGDLDLGLQAKLLRVLEGEEFCRVGGTTRVRIDV
                     RVVAATNKDLRAAIIQQAFRKDLFFRLSVLPITIPPLRERREDIPALVEHFLGHYCRE
                     LKRPPKRLTDGAWQVMTHHPWAGNIRELQNCIERAVILTDGDIIDEEQLGIRQHELSE
                     IHAREIPLEGTLHDVAAAAVRMAETRMIRKVLKSTGGNKSRASHILGVSYKTLLTKIK
                     EYGIERPAPPVSSDDHEPLAPAA"
     gene            complement(3118..3810)
                     /locus_tag="AC16BC_11370"
     CDS             complement(3118..3810)
                     /locus_tag="AC16BC_11370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3242259.1"
                     /translation="MLGEMSGAIAHEFRNYMGTILGWAKLLTKQVETGTQAGSMVEAI
                     RRELGMMQRLIDDLLSFGRRMEPQTERVDLRELVAQGVSLPPDRPDVRVETDWASGAA
                     TTAHWDPTLMRQVLKNLAQNAVDAMPDGGILRVSMGPSRAVTDAVEMLVSDTGSGIPA
                     EHAERIFEPFFTLKARGNGLGLALVRKIVTAHGGHITVQSKEGAGTTFCLTMPLQVRP
                     AATPTARLDRAA"
BASE COUNT          684 a         1264 c         1211 g          768 t
ORIGIN      
        1 ggtactccag gcggttgacc agcgtggcgg ggcttcgcga tggaaacgac cggacgagcg
       61 cgccatcgac cgagagcatg ttcccgtcaa tgctcgcgat cttgacacac tgggaggcat
      121 cgtcggctgg atcccacacg gtgccgatat cgagcaggag cacggtgtcg cccacttcaa
      181 acgtggctcc ggtatcaagc gctaattgat tggtcccctg aggagctgga tcccgcagaa
      241 aggtttcgcg atcatatggg ttggcggaaa actccgctcg ccctgagacg acctggatcc
      301 cggggctcgg gcatccggtg gatcgcggcg cagcgacgat cgtgtcgagt tccgcgcata
      361 tcagtgcgag cgcgaggtga gcttgctgct cgcggtcgat tccgtctgag gccgtcccat
      421 acgccgcata gaaggttgtc agcgcgtggc cgcacagccc cgcgaggatc aggccgacgg
      481 ccgtggcgac caacaactcc gcgagcgaca tcccgcgctc gtctactctg aaggtcgtcc
      541 tcttaacgtc aatcgccgtc atggcgttcg cccccggacg agcagcccgg aaatccgata
      601 cgtctggcgc ccgccgcgtc gggtccactg ggttactacc gacacacgtg tgagcccgtg
      661 gtccggctgt tctggttcca cataccatcg tcggatgacc ccgccgtcct catccccgcc
      721 gaacaggctg ccatccagct cctgctcgcc gggcgcaacc ggatccgtgg catgctcata
      781 tcccaaggcc agcctttcca tcaatttttc gcgggccgct ccgatggccc gttcccggcg
      841 ctgcgcctcc gccacgatcc gttgtccttc cgtaaaaccg gtcgcaacgg ccgtgagacc
      901 aaccgtgagc aggaacaggg cgatgagcgt ttcgatcaca ccgaaacctc cctcacggct
      961 tgcccggctc agggtcagtt ccactgtcgt ccatcccaga cggtgatcgc cgtgtggcca
     1021 gtcaggctga ccgtcacgcg acgggcggca ccccgacggg tcgtgatgct gatggaccca
     1081 atgctgctct gcggacctgg tcgcggggcg atgaagactg tgttgtcccg gaacgtcacg
     1141 ccgtcggatg ccggattgtc cggcaggcca aatcgcaatg tcggcgccag acggcgcgtc
     1201 tgcgtatggg tgtctggtcg cgaaaccgcg tagctccccg atcccggatc aacgatgagc
     1261 gtgagggttc ttccggacac ccgtgcctcg cgctggacca atcggatatc gccgaccatg
     1321 gttcgagcgg ccgaggatag ctccgcgcgc tcgatgagat cttcggtggc cggccaggcg
     1381 attgtcgcta cgattgcggc aatgcccaac accgttagga tttcgatgag cgtgtatccg
     1441 gagggagtgc gatttgacct gaccatgcca cgcggtaatc caaacagcgt gccatataca
     1501 gggctcgggg aaccagcgct tcgcggctgg gtcgtgggtc aagcctgatt aggcgttcag
     1561 caatgagtca atcactgtga accgttcgtc agggacggcg tggcggtcgc tcaggcggcg
     1621 tgggagtcgc ggaggatcgg actccagtgc gactttgatt gtacgattat ccggcgctca
     1681 ggctgcgggc gcgagcggct cgtggtcgtc cgaggacacc ggcggggctg ggcgttcgat
     1741 accgtactcc ttgatcttcg taaggagagt cttgtagctc acgcccaaaa tatgcgaggc
     1801 gcggctcttg tttccgcccg tgcttttgag gacttttcgg atcatccggg tctctgccat
     1861 ccgaaccgcc gcggcggcga cgtcgtgcag ggtgccttcc aacgggattt cccgcgcgtg
     1921 gatctcgctg agctcgtgtt ggcgaatgcc cagctgttct tcatcaataa tgtcgccgtc
     1981 ggtgagaatc accgctcgtt cgatgcagtt ttgcagttcg cgaatgtttc cggcccaggg
     2041 atggtgcgtc atgacctgcc acgctccgtc ggtcagccgc ttggggggcc gcttcagctc
     2101 ccgacagtag tggcccagaa agtgctcgac cagcgcgggg atatcctcac ggcgctcgcg
     2161 cagcggagga atcgtgatcg ggagaacact taggcggaag aacagatcct tgcggaaagc
     2221 ctgttggatg atcgccgctc gcaggtcctt gttcgtggcc gcgaccacac gcacgtcgat
     2281 cctgactcga gtcgttccgc cgacccggca aaactcctct ccctccaaga cgcgaagaag
     2341 tttcgcttgc agaccgaggt cgaggtcgcc gatctcatcg aggaaaatcg tcccgtgatc
     2401 cgcgagttcg aacttaccga tacgacgatc ggtcgctccg gtaaacgcgc cccgctcgtg
     2461 accgaacaac tcgctctcga gcagttcctt ggggatcgcc gcgcagttga ttgcgacgaa
     2521 cgggccttcc cggcgagggc tcagtccgtg caaggcgcgg gcgaagagtt ccttgcccgt
     2581 gccgctttcg cccaacaaca acaccgtggt ccggcttggc gcgacccgct gaatctgatc
     2641 cacgatcgcc aacacgggcg ggtgtttgcc gatgatgcgc ggcaagccgg catgcgcgga
     2701 ggcatcctct ttgagcaaga gattctccgc cacgaggcgt tgtttctcca gggcccgctc
     2761 gatgaccaat aagagatggt ccggatcgaa cggcttggtc aagaagtcgt atgccccgtc
     2821 ctttaccgcg cccaccgcgg tttcgatggt cccgtgcgcg gtcatcagca cgaccggcaa
     2881 gaggctgttc tgttccttcg cgacttggag aatatcgagc ccggtcttat acggcatctt
     2941 cagatcggtc accaccaagt cgattttccg gtcacgcagc cgttgaatcg cttcccggcc
     3001 gtcacgcgcc cagatcacgt cgtatccgtg accgctcagc gccgtcgtga gcatggcggc
     3061 cattccttcg ttgtcttcgg cgattaaaat cgacagcatc tcgcggcttc cttcgactta
     3121 ggccgcacgg tccagccgtg cggtcggtgt ggcggcaggg cgaacctgga ggggcatcgt
     3181 caggcaaaac gtggtgcctg ccccctcctt actctggacg gtaatgtgcc caccgtgcgc
     3241 ggtgacgatc tttcggacca aggcgagtcc caacccgttt cccctggcct tcaacgtaaa
     3301 aaacggctca aaaattcgtt ccgcatgctc cgcgggaatc ccgctgccgg tgtccgacac
     3361 cagcatctcc acggcgtcgg tcacggcgcg cgacggtccc atcgacacgc gaaggatacc
     3421 cccgtccggc atcgcgtcga ccgcgttctg cgccaagttc ttgagcacct gccgcatcag
     3481 ggtcgggtcc cagtgcgccg tcgtcgccgc cccgctggcc cagtccgttt ccacgcgcac
     3541 atcgggccga tccggcggga gactcacccc ttgggcgacc agctcgcgaa ggtcgacccg
     3601 ctcggtctgg ggctccatcc gccggccaaa cgacaggaga tcatcgatga ggcgttgcat
     3661 catgcccaac tcgcgccgaa tggcctcgac catcgacccc gcctgggtcc cggtttcgac
     3721 ctgtttggtc aacaatttgg cccatccgag gatcgtcccc atataattgc ggaattcgtg
     3781 ggcgatcgcc ccggacatct ccccgagcat tgccagggaa ggtgaagcct tgcagcgtcg
     3841 tgtggtccga ggtggcgaga ttgacctcgg tcgagaagcc gaagatcgca gtgccgatgg
     3901 gcacgctcgc cggggaccag ttgttgg
//
LOCUS       DBNGTT010000497         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1142098, whole genome shotgun sequence.
ACCESSION   DBNGTT010000497 DBNGTT010000000
VERSION     DBNGTT010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1142098"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1779)
                     /locus_tag="AC16BC_11375"
     CDS             complement(142..1779)
                     /locus_tag="AC16BC_11375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3242260.1"
                     /translation="MHAGTDETIAPAVTRRAVDRFHTLDRETFDVIVVGAGTGGLTVA
                     ALLARTGRSVLVLDQHSVAGGNATVFTRPGYAFDVGLHYLGDCGPDGGVPRILRAAGV
                     EDVAFREMDPDGFDTLMFPDFTFRVPKGLDAYRARLVERFPLDVKGIDRYLDVLQAIW
                     SFQGLGGGFAASVTTLWRARRAVPYLNATLQTLLDRCTRNTRLRAVLAGQSGDYAEPP
                     SRVSLVVHAMVVMGYLRGAYYPAGGGQVISDRLAGAIERHGGKILLSAPVTRIVIENG
                     RAAGVEFESRHLGHRAVRAPVVISDADVKETMLELVGAQHLKARTVARLYRYEMAPAL
                     GVAYLGVKRNLHAAGVPNTNFWIHPSYDQEPVYADARAGRFHPEPFCYVSIATLKDPD
                     NRRSAPPGVANIELATVVPSQPEAWGTTPEEIVKGTYHDNPAYRRAKDAFAARLIATA
                     DRVFPGLAKDVVFQDVSSPMTHTRYTRSTGGTAYGIALIPSQSLFRRVGHVTDVPGLY
                     LCGASTYTGHGITGVMWSGVLAASRIAGGKVLKEVMR"
     gene            complement(1779..2273)
                     /locus_tag="AC16BC_11380"
     CDS             complement(1779..2273)
                     /locus_tag="AC16BC_11380"
                     /inference="COORDINATES: protein motif:HMM:NF052504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sll1863 family stress response protein"
                     /protein_id="HIR3242261.1"
                     /translation="MGSSDEQSGTFETQLRAWGAELDKLKAKADKEVAEAKKEYFERV
                     ETLRKDIEARLLKWGYEVEEVTSNVAPEVRKTFQEFRAKVQKELKEWSPEIDMLKAKA
                     GKAEAEAKKLLEELKVKQGTLKQELGELKGASTAAWGDVKTGIGKAWDELKTGLHRAA
                     DKFK"
     gene            complement(2441..>2685)
                     /locus_tag="AC16BC_11385"
     CDS             complement(2441..>2685)
                     /locus_tag="AC16BC_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3242262.1"
                     /translation="LLIFQPTMLLSGFMFPVSSMPEAFQWVTLANPLRHYLEIVRGIF
                     LKGAGLDLLWPQYAALLAMGSAILWFASTRFRKRMG"
BASE COUNT          452 a          966 c          806 g          461 t
ORIGIN      
        1 gcccctccgg cccacgcgga cgcggtcaac aacagcgacg acaaaccggc catcacgaac
       61 acgaggacgt tgttcatggc gcacctctac ggttgactgt tgggcaacga actgtgtccg
      121 acacacccca cgtcatcggg atcaccgcat cacctccttc aagaccttcc cgcccgcgat
      181 tcgcgacgcg gccaacaccc cggaccacat cacccccgta atcccgtgtc cggtgtacgt
      241 gctcgccccg cacaggtaca ggccgggaac gtccgtgacg tgtccgacgc gacgaaacag
      301 cgactgcgac gggatcaacg cgatgccgta cgcggtgccg ccggtggagc gcgtgtaccg
      361 ggtgtgcgtc atggggctcg acacgtcctg gaagaccacg tccttggcga gcccgggaaa
      421 cacgcggtcc gcggttgcga tcagccgggc cgcgaacgcg tccttggcgc ggcggtaggc
      481 cggattgtcg tggtacgtgc ccttgactat ttcctccggc gtcgtccccc atgcttcggg
      541 ttgcgagggc accaccgtgg ccagctcgat gttggccacg ccgggcggag cggaccggcg
      601 gttgtccgga tccttgagcg tcgcgatcga gacgtagcag aacggctcgg ggtggaaacg
      661 tccggccctg gcgtccgcgt acaccggctc ctggtcgtac gacgggtgaa tccagaaatt
      721 cgtgttcggc acgcccgcgg cgtgaagatt gcgcttcacg cccagatacg ccacgcccag
      781 ggcaggggcc atttcgtacc gatacaggcg agcgacggtc cgtgctttga gatgttgggc
      841 cccgaccaat tccagcatgg tttctttgac gtccgcgtcg gaaatgacca ccggcgcgcg
      901 caccgcgcga tgccccaggt gccggctctc gaactcaacg cccgcggcgc ggccgttctc
      961 gatcacgatc cgggtcacgg gcgcggacag caggatcttg ccgccgtgcc gctcgatcgc
     1021 gccggccaac cggtccgaga tcacctgccc tccgcccgcc gggtaatacg cgccccgcag
     1081 atagcccatc accaccatcg cgtgcaccac cagcgacacg cggctgggag gctcggcgta
     1141 gtccccgctt tgtccggcca gcaccgcgcg cagtcgcgtg ttccgcgtgc agcgatcgag
     1201 caacgtctgc agggtggcgt tcagatacgg cacggcccgg cgcgcgcgcc acagggtggt
     1261 gaccgacgcc gcgaatccgc cgcccagccc ctgaaacgac cagatcgcct gcaagacgtc
     1321 cagataccgg tcgatgccct tcacgtccag gggaaaccgc tcgaccagcc gcgcgcggta
     1381 cgcgtcgagc cccttgggca cgcgaaaggt gaaatccggg aacatgagcg tgtcgaaccc
     1441 atcgggatcc atctcccgaa acgccacgtc ctccacgccc gcggctctga ggattcgggg
     1501 aacgccgccg tcgggcccgc agtccccgag atagtgcaac ccgacgtcga acgcgtatcc
     1561 gggtcgcgta aacaccgtgg cgttcccccc ggccacggaa tgctggtcga gcacgagcac
     1621 cgaccgtccg gtgcgggcga gcagtgccgc aaccgtgagc ccgcccgtgc ccgcgcccac
     1681 cacgatcacg tcgaacgtct cgcggtcgag cgtatggaaa cggtccacgg cccggcgcgt
     1741 cacggccggc gcgatcgtct cgtctgttcc ggcgtgcatc atttgaactt gtcggccgcg
     1801 cggtgcaacc cggttttgag ctcgtcccac gccttgccga tcccggtctt cacgtcgccc
     1861 caggccgcgg tgctcgcgcc cttcaactcg cccaactcct gcttgagggt tccctgcttc
     1921 accttgagtt cttccaacag tttcttggcc tcggcctccg ccttgcccgc tttggctttg
     1981 agcatgtcga tctccgggga ccattctttc aattccttct gaaccttcgc gcggaactcc
     2041 tggaacgtct tgcggacctc gggcgccacg ttcgatgtga cctcttccac ctcgtagccc
     2101 cacttcagga gccgggcttc gatgtccttg cgcaatgtct ccacccgctc gaagtactcc
     2161 ttcttggcct ccgccacctc cttgtccgcc ttggccttca acttgtcgag ttccgcgccc
     2221 cacgcgcgaa gctgcgtttc aaacgtgccc gactgctcgt ccgatgaccc catgtgcgct
     2281 cctttctgaa cgaccgcccg ttgtccggca ccccgttgcg cccatccgct tgtgcttcgc
     2341 gatgtcggga cggacggcaa cggctaccag tatggacggg agtccgatgg aaacccatcc
     2401 cccgaacgcg ggatgccggt gcaacccttg gcccgcgggc ctaccccatt cgtttacgaa
     2461 atcgcgtgga cgcgaaccag agaatggccg atcccatcgc caacagcgcc gcgtattgcg
     2521 gccacaacag gtcgaggccc gcgcccttga ggaagatgcc gcggacaatc tcgaggtaat
     2581 gacgcaacgg gttcgccagc gtgacccact ggaacgcctc cggcatgctg ctgaccggga
     2641 acatgaaccc cgacagcaac atggtcggct gaaaaatcag caggg
//
LOCUS       DBNGTT010000498         8166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2546688, whole genome shotgun sequence.
ACCESSION   DBNGTT010000498 DBNGTT010000000
VERSION     DBNGTT010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2546688"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1565
                     /locus_tag="AC16BC_11390"
     CDS             108..1565
                     /locus_tag="AC16BC_11390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3242263.1"
                     /translation="MIRRWLNRLNEMPLRGKVIAAFAGWTILVLGVTAGYVVSRFGAL
                     PGDLAPALLLQHHDAVGNVLWLVGALAVATVAFAWFVSGDILRPLTALASAVRGIKSG
                     SSHIPLPPVVTRDRDLWELTQAFDQMAHALGEREATLEQALRERERQLNELATIARFV
                     EGCATFRDGVDLYRHLTTHIAEALGAQICFVVRYEKGTHVMEAQTPGYGVGDEQLAPI
                     RYQVTPEIRSAWDFKAQGALLCNDPHSDQRVLRELIRSLGLYNLVAVPFYFQSRVSGL
                     IVVANKPKWVTHEDSRLLSVLANHASIAIANLYFYQEIQQLAIRDGLTGLHNYRYFRQ
                     CIARCVQQARRYESPVSLLLIDLDDFKKYNDAYGHLRGDQILKEVSAVLLAYTRAADL
                     VARYGGEEFVVLLPEIDEEQAHSVGEKIRQVIEAHPFTLATGELPQSLTISVGVASAI
                     GECSADALIHRADQALYRAKASGKNRVVASNISSS"
     gene            1665..2999
                     /locus_tag="AC16BC_11395"
     CDS             1665..2999
                     /locus_tag="AC16BC_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HIR3242264.1"
                     /translation="MAGIVPTVGGIWYAYSVARSGLFRETTVLIQADADTVAARINEA
                     VDVARAEVVHVAEQAQRARDARSKPRWEAAAAVLSTWGERAPVPLDTLTVIDSAGDVL
                     FGYALHRPGADLRVNPDHERTLTAQAASTGKVVFGGLVPGPDVERAAFAFAAPMPGGD
                     GTVLSSLYGSIPVEWVARSLNARSVEGGSVWVFDDAGRVVLATADRADGAETARALAA
                     QTASANQGVVRVQAGRTAWVGSSRPLGEFGARAPWTAVVAVPEAVIAAKINIWQYVGL
                     AAAAVLVLLGFAWLVSARIMRPISTLEDGTRRIAQGDVDFQVQINARNELDRLAGSVH
                     QMAYDLKRAQERLIKAERLAAIGEARIEISQELEGAAARLEGQVKRLQARPDLPAAAR
                     EPLGEIGEALAGIHASIQKLAHVPDRTSETVIRPRMPEAPSPSTEPRVEGVA"
     gene            2996..3424
                     /locus_tag="AC16BC_11400"
     CDS             2996..3424
                     /locus_tag="AC16BC_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242265.1"
                     /translation="MSAVSEAITDELLALLTDSLERERQLGRADRLQVQTAASTHLRQ
                     TWEAGWALKRAHEEALTRLLASAVHPAAPDVTPPATLPSPREMLSWAYDQERLLALQY
                     RDSARLAGHPETEKVLARLEDEQQRILDRVRVTYRDYSAA"
     gene            3511..4455
                     /locus_tag="AC16BC_11405"
     CDS             3511..4455
                     /locus_tag="AC16BC_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3242266.1"
                     /translation="MIQVRDLTKRYGPTTAIDRVGFEVRAGEVLGFLGPNGAGKTTTM
                     RIVTGFVPPTSGSVVVAGYDVLDQPLDAKRRIGYLPETPPVYPELTVLEYLRFTARIK
                     GVPRRAVSAGVDRVLGQCGLGDVRGRLIGNLSKGYRQRVGLAQALIHDPPVLILDEPT
                     VGLDPKQIIEVRELIRGLGGAHTVILSTHILPEVTATCQRVVIINEGRIVAVDTHEGL
                     AARLRKSEKIRVTLLRPGPDVEDVIRKLPNVVGVSADHAEGAEGRSWLVESDLGHDVR
                     EALARCAVTREWGLVELRPMGMSLEDVFLKLTAEDATT"
     gene            4452..5240
                     /locus_tag="AC16BC_11410"
     CDS             4452..5240
                     /locus_tag="AC16BC_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3242267.1"
                     /translation="MNAIRPILALAGKEIRVATASPILYVVAGVFLAIVGLFFSQIVT
                     IASLQSLQMMRFQGALPQLNLTVLVFQPMFRNIHVILLLVIPLLTMRLLSEERKHKTF
                     ELLMTSPITVTQLVAGKYLAVLALYAGLLAFTLYMPLLLGLWGPIDWPTVLSGYLGLL
                     LVGSVYLALGLVASSLTENQISAGVVALGLMLGWWLLGWAAVTAENPAVRAVLEYVSV
                     TSHFDRFIKGLLSLDSVVYSVSLAAFSLFLTHRVLDAGRWRETG"
     gene            5266..6816
                     /locus_tag="AC16BC_11415"
     CDS             5266..6816
                     /locus_tag="AC16BC_11415"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR3242268.1"
                     /translation="MAVPVNIGWLLAGGAVLSAAAVLTLDFSFPGRWYALAGYGTAAA
                     LLVGYAVAARRQLGSWGRRRSTRMGAHSALLAIVVGAILVMVNVLVVRHPARLDLSQT
                     GTFTLAPQSEKVLVGLKDDVKVTAFVQKGSETEAKFKDLLDTYRHYSQKLSSETVDPD
                     THPAAAKQYGITKYDTIVLEGGGRETRIQAVSEQDLTNALIRLGKTEKKRIAVLEGHG
                     EPSLTDPDVDGLSQAKDALERQGYEVAPLFLAQTGSVDAGTDAVIVADPKKPLLRQEL
                     DALKAYVSGGGRLLLLAGPGPKTGVDELAAQWGVTFREDTVIDPVSRLFGGDYTTPVI
                     RTYGDHEIVKDFRLATFFPLAQSLTFQPSPSAEVDYRVVALSSPESWGETQIVGGKAR
                     FDRGQDAQGPLDLVAALAPTSSAAPGESESKASRPAWRVVVSGNARFATNGFFNLSGN
                     GDLLVASINWLAEDQDLIAIRPKEASSIPVILTAGQERVVRWVPTLIVPGVIAGYGFA
                     VWRRRRRL"
     gene            6821..8158
                     /locus_tag="AC16BC_11420"
     CDS             6821..8158
                     /locus_tag="AC16BC_11420"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR3242269.1"
                     /translation="MRRYWGTLVLAVIAALLGLYFVFIDNPRERARLEQHDREGLVLA
                     LKEEDVTGLAIDTESDHLVLQRGDGTAWRVAAPVSAEADEGTVRRLLTQLTTMSVVRT
                     MEDVGDDASIGLAKPAVRVVVSRRDGSRAGVAFGDTTPTGAGVYARRDDGKVFLVATA
                     AKSTFEVSRDDIRRKEFVDFKPEIVTEIAITAQKRTLRVRRDGSEWRTDNPVRMADPE
                     KVTSLLSRLRALRATAFVDDAEHREGLRLSGEPRIGIDLSTADRTTALRFFSAADGAL
                     YARVGGETLYRVNERVVGELPLDAAALRDMRLVHAAFDDVQDVEVEQGATRYHVIRHA
                     SGWDLDGQPVEAAAVQAIEAMIRAFTAVRGESVASETVAATPSSAFKSPEALVTLRGD
                     GARTLAVVTIGGVSGEGRYAYSDPGGPVFVITSHALDQIPSKMTLETRGKPQG"
BASE COUNT         1340 a         2463 c         2840 g         1523 t
ORIGIN      
        1 gatgtcgggc gggcgttcct cttatggacc gatggccgcc cgcccgaccc ccaggcaccc
       61 ccatagcctt cccaggcgcg cgcgtttttg gcgcgagtgg agtagggatg atccgtcggt
      121 ggttgaatcg gctcaatgag atgccgctgc gcggcaaagt gatcgccgca ttcgcgggat
      181 ggacgattct ggtgctcggg gtgaccgcgg gatacgtggt ttccaggttc ggcgccctcc
      241 ccggagatct ggcgcctgcg ctcctgttgc agcatcacga cgccgtgggc aacgtgttgt
      301 ggctggtcgg ggcgttggcc gtcgcgaccg tggcgttcgc gtggttcgtg tccggcgata
      361 tcctgcgtcc cctcaccgca ctggcgtcgg ccgttcgcgg gatcaagtcc ggctcgtcgc
      421 atatcccttt gcctccggtc gtgacccgcg atcgcgacct gtgggagttg acccaggcgt
      481 tcgatcagat ggcgcacgcg ctgggcgaac gggaggccac gttggagcag gcgcttcgag
      541 agcgcgagcg acagctcaac gaactggcga cgatcgcgcg gttcgtcgaa gggtgcgcaa
      601 cgtttcggga cggcgtggat ctctaccgcc acctcacgac gcacatcgcg gaggcgctcg
      661 gcgcgcagat ctgtttcgtc gtcaggtatg aaaagggcac tcacgtcatg gaggcgcaga
      721 cgccgggtta cggcgtcggc gacgagcaac tcgctccgat tcgctaccag gtcacgccgg
      781 aaatccgttc agcctgggac ttcaaagccc agggcgcgct gctctgcaat gacccgcatt
      841 ccgatcaacg ggtcctgcgt gagctgattc gatcgctcgg gttgtataat ctcgtggcgg
      901 tcccgtttta tttccagagc cgggtgagcg ggctgatcgt ggtggcgaac aaaccgaagt
      961 gggtcaccca cgaggacagc cggttgttgt cggttctcgc caatcacgcc tccatcgcga
     1021 tcgccaacct gtatttctac caggagatcc agcagctcgc gatccgcgac ggcctgaccg
     1081 ggctgcacaa ctatcgctac tttcgacagt gtattgcgcg gtgcgtacag caggcgcgcc
     1141 ggtacgagtc gccggtgtcg ctgctgctca tcgatctgga cgacttcaaa aagtacaacg
     1201 atgcctatgg gcacctcaga ggggatcaga ttctcaagga agtctcggcc gtgttgttgg
     1261 cctatacccg cgccgcggat ctggtggccc ggtacggagg cgaggagttc gtggtgctgc
     1321 tgcctgagat cgacgaggag caggcccaca gcgtcgggga aaagatccgt caagtcatcg
     1381 aagcccaccc cttcacgctg gccaccgggg aactccccca gtccctgacc atcagcgtcg
     1441 gggtcgcctc ggcgatcggg gaatgctcgg ccgacgccct gattcatcgg gccgaccaag
     1501 cgttgtaccg ggcgaaggcg tccgggaaga accgcgtcgt cgcgtcgaac atctcctcct
     1561 cttgattccc tagtcggttc ccccgtatag tgaggcgcct cgtgactggc gaccccgcaa
     1621 tgttgcgatt cggtcttcga caccaactca tcggatggtt tttgttggct ggcatcgtgc
     1681 ccaccgtggg cgggatctgg tacgcctact ccgtggcgag gtcgggtctg ttccgcgaaa
     1741 ccacggtgtt gatacaggcc gacgccgata ccgtagccgc ccggatcaac gaggcggtcg
     1801 acgtcgcccg cgcggaggtg gtccatgtgg ccgaacaggc gcaacgcgcg agggatgccc
     1861 ggtccaagcc gagatgggaa gcggcggccg ccgtgttgtc gacatggggc gaacgcgctc
     1921 ccgttcccct cgataccctg acggtgattg attcagcggg cgacgtgctg tttggttacg
     1981 cgctccacag gcccggggcc gatctccggg tcaaccctga tcacgaacgg acgctgacgg
     2041 ctcaagccgc atcgaccggg aaggtcgtat tcggcgggct ggtgcccggc ccggacgttg
     2101 agcgggcggc ctttgccttt gccgccccga tgccgggagg tgacgggacg gtcctgagct
     2161 cgttgtacgg ctccattccg gtggagtggg tggctcggtc gctcaacgct cggtcggtcg
     2221 agggcggttc ggtgtgggtc ttcgacgacg ccgggcgcgt cgtgctggcc acggccgacc
     2281 gcgcggacgg agccgagacg gcgcgcgcct tggctgcgca gaccgcctcc gcgaatcagg
     2341 gcgtagtgcg cgtccaggcg ggtcgcacag cgtgggttgg gtcttctcgc ccgctggggg
     2401 agttcggcgc ccgcgctccg tggactgccg tggtcgcggt tcccgaagcg gtgatcgcgg
     2461 caaagatcaa catctggcaa tacgtggggt tggccgccgc ggcggttctg gtcctcttgg
     2521 gcttcgcatg gctggtcagc gcgaggatca tgcgtccgat cagcacgttg gaggacggca
     2581 cgcgccgtat tgcgcaaggc gacgtggatt ttcaggtgca gatcaatgcg cgcaacgagc
     2641 tggaccggct cgcgggctcg gtccatcaga tggcatacga cctcaaacgg gcgcaggaac
     2701 ggttgatcaa ggccgaacgt ctggcggcca tcggagaggc ccggatcgag atctcgcaag
     2761 agttggaagg tgcggccgcc cgcctggaag gtcaggtcaa gcgcctccag gcgcgtccgg
     2821 acctaccggc ggcggcgcgc gaaccgctcg gcgagatcgg tgaggcgttg gccggcattc
     2881 acgcctccat tcagaagctc gcgcatgttc cggatcgaac gtcggagacc gttattcggc
     2941 cgcggatgcc ggaggctccc agcccgtcga ccgagccccg cgtcgagggc gtggcgtgag
     3001 cgcggtgagc gaggcgatca cggacgagtt gctggcgctg ttgacggaca gcttggagcg
     3061 agagcgtcag ttgggtcgcg cggaccggct gcaggtgcaa accgccgcca gcacgcactt
     3121 gcgccaaacg tgggaggcgg ggtgggcgct caaacgcgct cacgaggagg cgctgactcg
     3181 actcttggcg tccgcggttc atcccgcggc gcccgatgtg accccgcccg caacgcttcc
     3241 gtccccgcgg gagatgttgt cgtgggcgta cgaccaagaa cggctgttgg cgttgcagta
     3301 tcgggattcc gcgcgtcttg ccggccatcc cgagacggag aaggtgctgg cccggcttga
     3361 ggacgaacag caacggatct tggatcgcgt gcgcgtgacc taccgcgact actcggcggc
     3421 gtagcgcgca ttgacgctcg tttgccccgc aacacttcgt tccggccttg acatcctcgc
     3481 gcagttttca gtagaaacgc ccacgaccgt atgatccagg tccgtgattt aaccaagcgc
     3541 tatggcccga cgacggcgat cgaccgtgtc gggttcgaag tgagggcggg ggaggttctt
     3601 gggttcctcg gccccaacgg cgccggcaag accaccacga tgcggatcgt gaccgggttc
     3661 gtgccgccga cgagcggttc ggtggtggtg gccggttacg atgtgctcga tcagccgctg
     3721 gacgccaagc gacggatcgg gtatctgccc gagacccctc ccgtgtaccc cgagttgacc
     3781 gttttggaat acctccgatt taccgcgcgg atcaaaggcg tcccgcgacg ggccgtctcc
     3841 gcaggagtgg accgcgtgct cggccaatgc gggctcggcg atgtccgcgg acggctgatc
     3901 gggaatctgt ccaagggtta ccgtcagcgg gtcgggctcg ctcaggcgct gattcacgat
     3961 cctcccgtcc tgattcttga tgagcccacg gtcggcctcg atccgaaaca gatcatcgag
     4021 gtccgcgagt tgattcgcgg tctgggcggc gcgcacaccg tcatcctgtc cacgcacatt
     4081 ctgccggagg tcacggccac gtgccaacgc gtcgtgatta tcaacgaggg ccggatcgtg
     4141 gcggttgata cgcacgaggg attggcggcc agactccgca agtccgaaaa gatccgggtg
     4201 acgctgctgc gtccggggcc cgatgtcgag gacgtgatca ggaaactgcc gaacgtggtg
     4261 ggcgtgtcgg cggaccacgc cgagggcgcg gaaggccgca gctggctcgt tgaatcggat
     4321 ttggggcacg acgttcgcga agccttggcc cggtgcgcgg tcacccgtga gtggggtttg
     4381 gtggagttgc ggccgatggg gatgagtctg gaagacgtgt ttttgaaact gaccgcggag
     4441 gacgcgacca cgtgaacgcg atccgaccga tactggccct tgcaggcaaa gagatccggg
     4501 tggcgacggc ctcgccgatt ctctacgtgg tcgccggcgt gttcctcgcg atcgtgggcc
     4561 ttttcttttc gcagatcgtg acgattgcca gtctgcaaag cctccagatg atgcggttcc
     4621 agggcgcctt gccgcaattg aacctgaccg tgctggtgtt ccagccgatg tttcggaaca
     4681 tccacgtcat cctgcttctg gtcatccccc tgctgaccat gcggttgctc agcgaagagc
     4741 gcaaacacaa gacgttcgag ctattgatga cgtccccgat taccgtgacg caactggttg
     4801 cgggaaaata tctcgcggtc ttggcgctgt atgcggggtt gctggcgttc acgctctaca
     4861 tgcccctgct cctcggcctg tgggggccga tcgactggcc gaccgtgttg tcgggctatc
     4921 tcggcctgtt gctggtgggg tccgtgtacc tcgcgttggg actggtggct tcgtcgttga
     4981 cggaaaatca aataagtgcc ggagttgtcg cgctcggcct gatgctgggg tggtggctgt
     5041 taggttgggc tgcggtgacc gcggaaaatc ccgcagtgcg agccgtcctc gagtatgtat
     5101 ccgtcacctc acattttgac cgctttatca agggcctgct gagcttggat agcgtcgtat
     5161 actccgtgag cttggcggcg ttcagtctct tcctgaccca tcgggtattg gacgcgggac
     5221 gatggcgtga aacggggtga ctcagtgagc tccgagttct gagtgatggc ggtgccagtg
     5281 aatattggat ggctcttggc gggcggcgcg gtcctgtcgg cagcggcggt gctgaccttg
     5341 gacttttcat ttcccggccg gtggtacgcg ttggccggct atggcacggc tgcggcgctc
     5401 cttgttgggt acgccgtggc ggcgcgccgg caactcggct cgtggggccg ccgacgatcc
     5461 acccggatgg gcgcccacag cgcgctgctc gcgatcgtgg tcggggcgat cctggtcatg
     5521 gtcaacgtgc tggtggttcg ccaccccgcg cgcctcgacc tgtctcagac cggcacgttc
     5581 accttggccc cgcagagcga gaaagtgttg gtgggtctca aggacgacgt gaaagtgacg
     5641 gcgttcgttc agaaaggcag cgagacggag gcgaaattca aggacctgct cgatacgtat
     5701 cggcactata gccagaaact ttccagcgaa accgtagacc ccgacacgca tccggccgcg
     5761 gccaagcaat atggcatcac gaagtacgac acgatcgttc tcgaaggcgg aggccgcgaa
     5821 acccggattc aagccgtgag cgagcaggat ctcaccaacg cgctgatccg gcttgggaag
     5881 acggagaaga agcgcatcgc ggtgctggaa gggcacggcg aaccgagcct gaccgatccc
     5941 gacgtcgacg gcctctcgca agccaaggat gcgctcgaac ggcaggggta cgaagtcgca
     6001 ccgctgttct tggcgcaaac gggatcggtc gatgccggaa ccgacgcggt catcgtcgcg
     6061 gacccgaaaa aaccgctgct gcgccaggaa ctcgacgccc tcaaggcgta tgtgtcgggc
     6121 gggggacgtc tgttgttgct ggccgggccg ggcccgaaga ccggggtcga cgaacttgcg
     6181 gcgcaatggg gggtcacgtt tcgcgaggac acggtgatcg acccggtgtc gcggttgttc
     6241 gggggcgact ataccacccc ggtgattcgt acctatggcg atcatgagat cgttaaagat
     6301 tttcgtctgg cgacgttttt cccgttggcg caatcgctga cgttccaacc gtctccgtcc
     6361 gcggaggtcg actatcgcgt cgtggcgctc tcgagtcccg agagctgggg ggagactcag
     6421 atcgtggggg gcaaggcgcg gttcgaccgg gggcaagacg ctcaaggacc gctggacctc
     6481 gtcgcggcgc tcgctccaac gtcgtcggcc gcccctggtg agagcgagtc caaggcctcg
     6541 cggccggcct ggcgcgtcgt ggtgtccggc aacgcgcggt ttgccaccaa cgggttcttc
     6601 aacctgtcgg gcaacggcga tctcctggtc gcgtcgatca actggttggc cgaagaccag
     6661 gacctcatcg cgatccggcc caaagaagcg tcctcgatcc cggtgatcct gaccgcgggg
     6721 caggaacgag tggtgcgttg ggtgcccacg ctgattgtcc cgggcgtgat tgccgggtac
     6781 gggttcgccg tgtggaggcg gcgccggcgg ctgtaacgcc atgaggcgct actggggaac
     6841 gctggtcctg gcggtcatcg ccgcgctact gggcctgtat tttgtcttca tcgataatcc
     6901 gcgcgaacgc gctcggctcg aacaacacga ccgcgaaggc ctcgtcctcg cgctcaaaga
     6961 agaagacgtg acggggttgg cgatcgacac cgaatcggat caccttgtcc tgcaacgcgg
     7021 agacgggact gcctggcgcg tggcggcgcc ggtttcggct gaggccgacg aggggacggt
     7081 tcggcggttg ctcacccagc tcacgaccat gtccgtggtc cgcactatgg aggacgtcgg
     7141 ggacgacgcg tcgatcggtc tggccaaacc cgcggtgcgt gtcgtggtga gccggcgcga
     7201 cgggagccgc gccggggtgg cgttcggcga caccactccg acaggggccg gagtctacgc
     7261 gcgtcgcgat gacgggaagg tgttcctggt cgcgaccgcg gccaaatcga cattcgaggt
     7321 gtctcgcgac gacatccgtc gcaaggagtt cgttgatttc aagccggaaa tcgtgaccga
     7381 gatcgccatc accgctcaga aacggacgct gcgcgtgcga cgagacggga gcgagtggcg
     7441 gaccgacaac ccggtgagaa tggcggaccc cgagaaggtg acgtcgctgt tgagccgttt
     7501 gcgtgcgctc cgtgccaccg cgtttgtcga tgatgcggag catcgcgagg gattgcggtt
     7561 gagcggggag ccccgcatcg gaattgatct gtcgacggcc gaccggacga cggcccttcg
     7621 gttcttcagc gcggccgacg gggcgctcta tgcacgcgtc ggcggggaga cgctctatcg
     7681 ggtcaacgag cgggtggtcg gggagttgcc tctggacgcg gcagccctgc gcgacatgcg
     7741 gttggtgcac gccgcatttg acgacgtgca ggacgttgag gtggagcagg gagcgacgag
     7801 gtaccacgtg atccgtcacg caagcgggtg ggatctggat ggccagcccg tggaagccgc
     7861 ggccgtccag gcgatcgagg ccatgatccg ggcgttcacc gccgtacgcg gtgaatccgt
     7921 ggcgtcggag accgttgccg cgaccccgtc gtccgcgttc aagtcccccg aggccctggt
     7981 gacacttcgg ggtgatggcg cccgcacgct cgcggtcgtg acgatcggcg gtgtcagtgg
     8041 cgagggacga tatgcctatt ccgatccggg aggtccggtg ttcgtgatta cttcgcacgc
     8101 cttggaccag attccttcga aaatgacgct ggaaacgagg gggaaacccc agggttaacc
     8161 ccgacc
//
LOCUS       DBNGTT010000499         1758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2164910, whole genome shotgun sequence.
ACCESSION   DBNGTT010000499 DBNGTT010000000
VERSION     DBNGTT010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2164910"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="AC16BC_11425"
     CDS             complement(<1..388)
                     /locus_tag="AC16BC_11425"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HIR3242270.1"
                     /translation="MNEARRVVRAAGVIGAATFLSRVLGFVRDMVVARAFGASQVADA
                     FYVAYRIPSLLRELFAEGSMSAAFVPVFTQTLTTDSREEARRLARAAFSLILVIVATV
                     TVIGMAIAPWLVAVIAPGFGDDPGKAA"
     gene            544..813
                     /gene="rpsT"
                     /locus_tag="AC16BC_11430"
     CDS             544..813
                     /gene="rpsT"
                     /locus_tag="AC16BC_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR3242271.1"
                     /translation="MGVHASAIKKDRQALRRRQHNRTMMAALKTAVKKVRSAVTTKNA
                     AEAKQALAEAVPTLAKAASKKLIHPNRASRLISRLTRHTNRVNAG"
     gene            complement(760..>1758)
                     /gene="holA"
                     /locus_tag="AC16BC_11435"
     CDS             complement(760..>1758)
                     /gene="holA"
                     /locus_tag="AC16BC_11435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3242272.1"
                     /translation="DEALSALRRHAAAPDDLMNSHVFSGSEITPAELVAIAETLPALA
                     PRRFIVVRDAERLASSDVLSGYCGDPSPTTCVVFLSAKLDRRKSWASALVKRAVTVAC
                     DPLKPAALRTWVQREVSRRGLRLNEEALTYLLARADGSLRAMSQDLEQLALGRPDATR
                     PLSVDDLSVLSPGEAAVSVFDWAHAVAMGRTSDALAFARRLLQDEAPLLMLSILAGQW
                     RKMLRYRALVDGGATAAQASSALELPPFAASRVADAAKRRTAAQLIAGLTWCLETDAA
                     VKGGALAPPLAMERLVGVLCDGMTPPPGRSLTGSWWPGLSARVDAVGVAGQARNQA"
BASE COUNT          321 a          612 c          586 g          239 t
ORIGIN      
        1 acgcggcctt gccgggatcg tcgccgaacc ccggggcgat cacggccacg agccacgggg
       61 cgatcgccat cccgatgacg gtcacggtgg ccacgatcac cagaatcaag ctgaacgcgg
      121 cccgggccaa tcggcgggct tcctcgcgtg agtcggtcgt gagcgtctgc gtaaatacgg
      181 ggacgaacgc ggccgacatc gagccttcgg cgaacaattc ccgcagcagg ctggggatcc
      241 ggtacgcgac gtagaacgcg tccgccacct gactggcgcc gaatgcccgc gcgaccacca
      301 tatcccggac gaatcccagg acgcggctga gaaacgtcgc ggcgccgatc acgcccgcgg
      361 ctctcacgac ccggcgggct tcgttcatgg cgcgggcgcc gtgggaggcg cgtctgggct
      421 gggatgaccg gctgtgatct tgacttcgcc cacgtcagcg tgctacagat tcctacttcg
      481 attcgttgct gcggcgaatg gacgccccga cgagacgttg gtgaaggaca aggagaaggg
      541 acgatgggag ttcacgcctc ggcgatcaag aaggaccggc aagcgctccg ccgccggcaa
      601 cacaaccgca ccatgatggc ggcgctcaag accgccgtga agaaggtccg aagcgcggtc
      661 accaccaaaa acgcggcgga ggccaagcag gcgctcgcgg aagcggtgcc cacgctcgcg
      721 aaggccgcgt ccaagaagct tattcatccc aaccgcgcct cacgcttgat ttcgcgcctg
      781 acccgccaca ccaaccgcgt caacgcgggc tgaaagtccc ggccaccacg agccggtcag
      841 gctgcgaccc ggcggcggcg tcatcccgtc gcacaaaacg ccgaccagcc gttccatggc
      901 cagcgggggg gccagcgccc cgcctttcac ggcggcatcc gtctccaaac accacgtcaa
      961 tcccgcgatg agttgggcgg cggttcgccg tttcgccgcg tccgccaccc ggctggccgc
     1021 gaacggcggg agttccagcg cgctcgacgc ctgagccgcg gtggccccgc cgtccaccag
     1081 ggcgcgatac cgcagcatct tccgccattg accggccagg atcgataaca tcaagagcgg
     1141 ggcttcgtcc tgcaacagac gtcgcgcgaa cgcgagcgcg tcggacgttc ggcccatggc
     1201 cacggcgtgg gcccaatcaa acaccgacac cgcggcttcc ccgggggaga gcaccgaaag
     1261 gtcgtcgacg ctcagcggtc tggtcgcatc gggccggccc agcgcgagtt gttcgaggtc
     1321 ttgcgacatc gcgcgcagac tcccgtcagc ccgggcgagc aggtacgtca acgcctcctc
     1381 gttgaggcgg aggcctcgtc gggagacctc ccgctgcacc cacgttctga gcgcggcggg
     1441 tttgagcggg tcgcaggcca cggtgacggc gcgtttgacc aaggccgagg cccacgactt
     1501 acgccggtca agcttggcgg acaggaagac cacgcaggtg gtcggtgagg gatcgccgca
     1561 gtagccggac aagacatcgg acgatgcgag tcgttccgcg tcgcgcacca cgatgaagcg
     1621 ccgcggcgcc agagccggaa gcgtctcggc gatggccacc aattccgctg gcgtgatttc
     1681 cgaacccgaa aacacgtgcg agttcatcag gtcgtccggc gccgccgcgt gccgccgcaa
     1741 ggcggagagc gcctcgtc
//
LOCUS       DBNGTT010000500        21383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3185845, whole genome shotgun sequence.
ACCESSION   DBNGTT010000500 DBNGTT010000000
VERSION     DBNGTT010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 21383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21383
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3185845"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..326
                     /gene="rpmE"
                     /locus_tag="AC16BC_11440"
     CDS             120..326
                     /gene="rpmE"
                     /locus_tag="AC16BC_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR3242273.1"
                     /translation="MKADIHPTYQEALITCACGSVLKTRSTVKDIRVEICAMCHPFFT
                     GMQKIVDTEGRVERFRKKYSNNKK"
     gene            428..1495
                     /gene="prfA"
                     /locus_tag="AC16BC_11445"
     CDS             428..1495
                     /gene="prfA"
                     /locus_tag="AC16BC_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3242274.1"
                     /translation="MILRLETLASRFAEVERNLGDPSVIADPQALQRLSKERAELADV
                     VGQYQAYCALVTELEQTRRLAEDPRSEPTFRELALDEAQTLEVKRAELYDALRIALLP
                     KDPRDEKNIFLEIRAGTGGSEAALFAAELFRMYGKYAETKRWRVELVSSSETGIGGMK
                     EAIVLIEGKGAYSRLKFESGVHRVQRVPVTEASGRIHTSTVTVAVVPEAEEVEYHIDA
                     KDLRIDTFCSSGPGGQSVNTTYSAVRITHIPTNTVVSCQDERSQTKNRAKAMRILRSR
                     LVEVERERQEAEIAKDRKAQVGTGERSEKVRTYNFPQNRVTDHRIGLTLHRLDQVLNG
                     DLDELIDALQAWTQTAQLQRL"
     gene            1462..2358
                     /gene="prmC"
                     /locus_tag="AC16BC_11450"
     CDS             1462..2358
                     /gene="prmC"
                     /locus_tag="AC16BC_11450"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3242275.1"
                     /translation="MDADRATATTLSPLTRGAAVQWASGLLAAAGVESPRVEADALLS
                     EAVHAPRYASYLEPDATLAESAHERFVAMVARRAQREPIQYVTGRETFCGLDLVVTPD
                     VLIPRPETEGVVAAALDAIGDRGRPVVADVGTGSGAIALAVAAARPDAVVYAADRSAR
                     ALDVARRNAERLALSDRVQCVEGDWFEPLIERGVRADVLVSNPPYVADAEYARLQAEV
                     RFEPAQALLGGPDGLAFYRRIAAESRHVLAPGGRIVIELGFGQADAVRTIAEQAGFAI
                     ERLDDDANGIARVMTWAEATWT"
     gene            2349..3611
                     /gene="murA"
                     /locus_tag="AC16BC_11455"
     CDS             2349..3611
                     /gene="murA"
                     /locus_tag="AC16BC_11455"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3242276.1"
                     /translation="MDLIAVQGGRRLVGEIPISGAKNAALPLLASALLSQEECVLTNV
                     PNLRDVNTTLKLLRHLGASAQADGAGRVSVTAGTVSSADAPYELVKTMRASILVLGPL
                     IARCGEARVSLPGGCAIGARPVNLHLSGLERMGAEVSIEHGYIRVKAGTLRGARVALD
                     LPTVTGTENLLMAATLAEGTTTIEHAAREPEIIDLANALIARGAKIQGAGTDTITVDG
                     VASLRGGQYRVMPDRIETGTYLAAAAITGGDVFLRGGVGSHLEAVLAKLREAGVEVRA
                     EADGLRVTRRGSLRGVDVTTAPYPGFPTDMQAQMMALLSVADGASVLTETVFENRMTH
                     VAELQRMGASIRVEGNHAVVRGVPRLSGAPVMASDLRASACLVVAGLAAEDETMISRV
                     YHLDRGYERMEEKLTRVGAVIRRVREAA"
     gene            3608..4261
                     /gene="hisG"
                     /locus_tag="AC16BC_11460"
     CDS             3608..4261
                     /gene="hisG"
                     /locus_tag="AC16BC_11460"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR3242277.1"
                     /translation="MSDAKLVIALPKGRLLEPTVELFTRIGIRPKGLTRDTRQLLFRT
                     DREGVSVMVVRAVDVPTYVEYGAADLGVAGKDVLLEHPKDVYEPVDLGYGLCRVVWAA
                     PAGRPGTWAGSRAGKLRVATKYPNIADRYFTQRGVPIEMVKLWGAVELAPRIGLAESI
                     VDLVSTGKTLEENGLTIVEEIVRSTARLIVNRASMKVKHSRIGELVERVTAVCPSPT"
     gene            4297..5589
                     /gene="hisD"
                     /locus_tag="AC16BC_11465"
     CDS             4297..5589
                     /gene="hisD"
                     /locus_tag="AC16BC_11465"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3242278.1"
                     /translation="MKVIKLKGSAGRRELDKIVNRGEPDDRRVETKVRAMLDDVRAKG
                     DVAVSRYTKQFDHIAMSPKRFLVTIEERAAALAKTDPKVVEALKLAADRIYAFHSKQQ
                     SHSWSFEEAGITLGQQLRPLASAGLYVPGGKAAYPSSVLMNGIPAKVAGVERIVMCTP
                     TPRGEMNPYVLVAADLVGIHEVYRIGGVQAIGAMAYGTKTIKRVDKIVGPGNAYVATA
                     KRLVYGTVDIDMVAGPSEILIIADETAHPAHIAADILSQAEHDEDAWTILIALSDGFV
                     KKVQKEITLQLAKLDRRKIAEASLKHRGVAMVVPEVVDAIGIANEIAPEHLELSIERP
                     YKVLKFVKNAGSIFLGHHTPQALGDYLAGPNHVLPTGGTARFFSPLSVDDFTKKSGLI
                     AFSQDALRRFSEKLVQIARVEGLEAHAQAVEIRMRPED"
     gene            5592..6233
                     /gene="hisB"
                     /locus_tag="AC16BC_11470"
     CDS             5592..6233
                     /gene="hisB"
                     /locus_tag="AC16BC_11470"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR3242279.1"
                     /translation="MPGRAATRRASGSSRRLPARRAAISRKTKETAIVVTLDLDGTGR
                     TRVATAMPFLDHMLTVMGKHGLLDLTVKASGDLDVDAHHTVEDLGIVLGQALKQALGD
                     KAGITRFGAGAVPMDETLARATVDVSGRPYLVYRVPVTTRKIQSFDTELVEHFFEALV
                     VHAGLTLHLEVPYGKNAHHMLEATFKAFGRALEQATRIDPRVAGVPSSKGRIE"
     gene            6363..7007
                     /gene="hisH"
                     /locus_tag="AC16BC_11475"
     CDS             6363..7007
                     /gene="hisH"
                     /locus_tag="AC16BC_11475"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3242280.1"
                     /translation="MIAVIDYQVVNLRSIQKAFERLGVEVEVTSDPARIRRAERVVLP
                     GVGAFRAGMDQLARLGLVPVIRDVIDAGTPFLGICLGMQLLMTQSEEFGVHRGLHVIP
                     GKVVRFPSETPAMKGLKIPHMGWNAVVMRRRPDALDGMPDRGYFYFVHSYYVVPDDPA
                     VIAADTTYGVRFPSILAWRNVVACQFHPEKSQALGLSLLRRFASFRPVATGAAL"
     gene            7004..7729
                     /gene="hisA"
                     /locus_tag="AC16BC_11480"
     CDS             7004..7729
                     /gene="hisA"
                     /locus_tag="AC16BC_11480"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3242281.1"
                     /translation="MIIIPAIDIRGGRCVRLLQGRFDAETVYGDNPAAMAERWQVEGA
                     VRLHVVDLDGAADGVPKNRDAIAAVVKAARIPVQVGGGVRDVTTIDEYLTWGVDRVVL
                     GTRAALDPAFLRDVCKRFPGKVVVAIDARDGRVAIRGWLEATDRDALDVAREASAAGA
                     ASILYTDIKRDGTQEGPNLEALAAVAAATPTPVIASGGVSTPEHVRSLARVKGVEAAI
                     VGQALYRGTLSLRDAMAATESSR"
     gene            7928..8725
                     /gene="hisF"
                     /locus_tag="AC16BC_11485"
     CDS             7928..8725
                     /gene="hisF"
                     /locus_tag="AC16BC_11485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR3242282.1"
                     /translation="MTTQQAPVSGGSVTFAKRIIPCLDVKDGRVVKGVRFVDLRDTGD
                     PVEAATSYDGEGADELCFLDITASSDERPILLDVVRRTAERAFMPLTVGGGVRSVEDI
                     RALLLAGADKVSINSAAVARPDLLRESAERFGSQCIVIAIDARRGERGWEVLTHGGRR
                     AAELDAVEWAVRATTLGAGEVLLTSIDRDGTQQGYDLELLRAVSSAVPVPVVASGGAG
                     TLEHFYDALTTGGADAVLAASLFHDRRLTVGQVKQYLAERGVVVRPV"
     gene            8902..9621
                     /gene="hisIE"
                     /locus_tag="AC16BC_11490"
     CDS             8902..9621
                     /gene="hisIE"
                     /locus_tag="AC16BC_11490"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR3242283.1"
                     /translation="MAKKKEVDSLWSAIKFDAAGLIPAVVQERGSGRVFMLAYMNRES
                     LELTLKTGFTHFFSRSRQALWKKGETSGHTQRVESVRLDCDGDTLIVEVEQTGPACHT
                     GEPTCFFRLVDGEQLTPDPDGGGAVLDRVYRVIQDRKAHPVEGSYVASLLAGGVDRIL
                     KKVAEEAGEVVLASKNGARDQIVWEVADLWFHTLVALGHHDIPPSAIYDELTRRFTHR
                     APAPRKRPATKPKRALRKPAR"
     gene            9658..10008
                     /locus_tag="AC16BC_11495"
     CDS             9658..10008
                     /locus_tag="AC16BC_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR3242284.1"
                     /translation="MSASNCLFCRIVRRELPATIVHEDDEVVAFQDIAPKAPVHFLVI
                     PKRHLDGLQAVDAGNASIMAPIFACIGRLAVQFGVAKSGYRTVINAGADGGQTVFHLH
                     VHVLGGRPMTWPPG"
     gene            10088..10552
                     /locus_tag="AC16BC_11500"
     CDS             10088..10552
                     /locus_tag="AC16BC_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009657508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3242285.1"
                     /translation="MALQERLVEEMKEAMRSGQAAKLSVIRMVRSALKNREIDRGKDK
                     PLTDQDVLDVIQSGIKQRREAIEQYEKGGRAELVAKENEEIAILQTFLPAQLSGPELD
                     ALVKAAAAEVGASSMKDMGKVMKSVMARVAGRAEGGAVSAAVKRTLERLPPP"
     gene            10562..12451
                     /gene="dnaG"
                     /locus_tag="AC16BC_11505"
     CDS             10562..12451
                     /gene="dnaG"
                     /locus_tag="AC16BC_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3242286.1"
                     /translation="MAPDRPATERPGQSPGATRHAGGFGDAELRAIRERVDLVEFIGA
                     YVSLKRTGQNAVGLCPFHSDKRPSFTVSASKQLYHCFGCGAGGDLFAFVMQRESLSFP
                     EAVQWLARKSGVPLPAHERSDEDRRRQSLYDIHAAAADHFRRRLAGREGETARRYLHG
                     RRLTADTVEAFGLGYAASSWDDLLAALSAKGWTSAQVQDAGLIVPRQQGGGWYDRFRH
                     RVICPIRDAHGQVVAFGGRVLDDAQPKYLNSPETPLFSKGRQLFALDRARRSVAANGT
                     LVVVEGYFDVIAAHQAGFTNVVATLGTALTPAHLEAIRRLVNRVCLVFDPDPAGVKAA
                     LRTVDLFAPLDLRVDVASLPGGKDPDRVIHEDGPDSFRAALEQAVGLTQFVLGHLVPE
                     GSYRDPGAKKAAVARASPLIAAMTNAIDRDHYLRWLADRVGVAESTLREELRRGRNPA
                     APRTVAATVTPRGDAGKPVAGWPRAEEVVAAAVIHGRLAPALMADVSGDAMTDPTLRA
                     VVELVERETRSGDGAAAVIARDRALAEGRRDVAEALAKLATYEFLGDDPDQEIFDCVV
                     TLRIDQRRRRVEELTRAIHRHEAQGKNDEAQRLQGEHVQLTKELSGLRLLRGSRMGPA
                     QAAGR"
     gene            12468..14288
                     /gene="rpoD"
                     /locus_tag="AC16BC_11510"
     CDS             12468..14288
                     /gene="rpoD"
                     /locus_tag="AC16BC_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR3242287.1"
                     /translation="MSKDEKLDEVKNLISLGKEKGFLTYDELNNALPADVLSSEQIDN
                     LMVMFGEMDIEVVDSGEESRFPQMEEAEEELDELEEGEEVGEEFLEESEEKEKEIDLT
                     PGILSRTDDPVRLYLKEIGSVHLLSREGEIEIAKRIEEGKREVSSIVFGMPMTVKEVL
                     ASGELLKQGVIAVEDLVVAFDDDLGEQEEGPRDTTEIKAKVLQMMGGIRAACRDLNHV
                     AIRWKRTGGDPAKRKKIKPAVKEAKDRLVEKMEALNLQPRVLDEVVKRLKTLADDIER
                     SEREMADVRRALGHPDEEPLLIFRRMRHNPRALKAALKKTELSEESFTQAEKRYRNAL
                     RLIRTAEMEAAVSAPELKDAIRQLYLGDLRIKRAKGELVEANLRLVVSIAKKYTNRGL
                     QFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMI
                     ETINKLIRTSRLLVQELGREPLPEEIAEKMDLSLDKVRKILKIAKEPISLETPIGEEE
                     DSHLGDFIEDKRVASPLEATIRYDLQRQIGKVLGTLTTREEKVLRKRFGIGETTDHTL
                     EEVGQDFEVTRERIRQIEAKALRKLRHPSRSRRLKSFVEG"
     gene            14404..14477
                     /locus_tag="AC16BC_11515"
     tRNA            14404..14477
                     /locus_tag="AC16BC_11515"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14438..14440,aa:Ile,seq:cat)
     gene            14647..15375
                     /locus_tag="AC16BC_11520"
     CDS             14647..15375
                     /locus_tag="AC16BC_11520"
                     /inference="COORDINATES: protein motif:HMM:NF049746.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3242288.1"
                     /translation="MNEQLALLIRLQELDSQIDALAEQRRQAPALVEAAAKPVQEAEA
                     ELVKTKAAFDVLTRDRRDKEQELKEREDRIEKLKGRSGDIKSNKEYQAHLFEIELAQQ
                     EKGTVEERLLEMMDRIEAMQATVKSQEAAVAVCKREFDKERARLEGEQRAAQAEADRL
                     VADAKSLAEQLAPANRQAYERLKTTRKGLAVVAVKNGACQGCRLALPPQLIADLRKNE
                     KLLTCTYCHRMLFWPVSDLAAQKT"
     gene            15467..16708
                     /locus_tag="AC16BC_11525"
     CDS             15467..16708
                     /locus_tag="AC16BC_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3242289.1"
                     /translation="MTPVSRPSRATAKALRETNRELDVLRRISETISCTLDQEAVLRQ
                     IIDLVVEVTSCDAAFLYLVDEGGKELVLRASKQPHPRLIGRIKLEMGEGITGWVARER
                     TPVAIARHASDDPRFKYFQKLPEDRYQAFLSVPVIAKNEVIGVINVQHREPHRHTASE
                     IALLSAIGHQVGGAIENASLYDAMRRKTLQLETLLQVSTTITSTRYLEEMLPLIVTIT
                     ARTMGSAICSIMLTDEQRGELRIAATQSLSDAYRGKPSLKIGEGVSGRAVRDKRPIAV
                     TDVTEDPAYVYRELARREGLRSLLSVPMLYKDRAVGVINSYTAEPHVFRPDEIELLEA
                     VANQAAVAIENTRLMQETAQMHDALELRKLVEKAKGILMRERRLTEEEAFRVLQRQSM
                     NNRWPMKQVAEAVIGTARGRT"
     gene            complement(16916..17089)
                     /locus_tag="AC16BC_11530"
     CDS             complement(16916..17089)
                     /locus_tag="AC16BC_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242290.1"
                     /translation="MAPGRERAKELRRRQKRRRERLRERVKTLKAKSASQAAKKPAVK
                     AKPKKEKTAAPSA"
     gene            17268..17768
                     /locus_tag="AC16BC_11535"
     CDS             17268..17768
                     /locus_tag="AC16BC_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3242291.1"
                     /translation="MTPRSKTKTRSRELLHERLREHGLKFTHQRELIAELFLEQDHIT
                     VDELFQKVSKRDPSIGVITVYRTLKLLCQWELAQERHFGTGKTIYDNVAWKSHHDHLI
                     CTHCGRIIEFENFQIEQFQEQVARQHDFRITFHKLELYGICKACESEERAKGRATRSV
                     PHHHRH"
     gene            complement(18229..18468)
                     /locus_tag="AC16BC_11540"
     CDS             complement(18229..18468)
                     /locus_tag="AC16BC_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242292.1"
                     /translation="MPTNPRSGATTKNPGVDAKESPIHPTTASNASTGTHVNRDIDTD
                     IRRPTTMPHANLNNRAAASNWSAVRCNVTALIRTW"
     gene            complement(18850..19170)
                     /locus_tag="AC16BC_11545"
                     /pseudo
     CDS             complement(18850..19170)
                     /locus_tag="AC16BC_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
     gene            complement(19311..19601)
                     /locus_tag="AC16BC_11550"
     CDS             complement(19311..19601)
                     /locus_tag="AC16BC_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242293.1"
                     /translation="MSQQTIVNRLKSLEELDGISVEVSGSTSGTVRVIHKKHHAPEFL
                     FRWYSDHFVGYFIDSDGKQSQAVVSLYSPMDAIRFVSAYATLNDIRANQKSA"
     gene            complement(20108..20986)
                     /locus_tag="AC16BC_11555"
     CDS             complement(20108..20986)
                     /locus_tag="AC16BC_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3242294.1"
                     /translation="MRRFWVIAPYDARYPDEWEQVWKYDLEHGVISIGWPALGDVSAL
                     AEDELLRLVRRTYRDYKPAAATLSSGMLHKFYHSVQRGDMLVARRGLKQLAAVGTVKG
                     AGYYDPNKHSEPYDSPDEAFPNHLDVQWANKPRDVVFPEIVFSIQTIYEIPQEKFLSL
                     TESSRRMIPPDSNFPDEVGDDEEFIEGAVCQRTVNAYERDPRARAACIAKHGLRCGVC
                     GMSFERRYGSIGRGFIHVHHKKPLAGRGGEYKLRPTVDLAPVCPNCHAMLHTQEPPLS
                     IEELRTIMQQRNGKKR"
     gene            21103..>21383
                     /locus_tag="AC16BC_11560"
     CDS             21103..>21383
                     /locus_tag="AC16BC_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HIR3242295.1"
                     /translation="MTPTGSETSLKASAEAKPKLLDQLRHTIRLRHYSIRTEEAYVQW
                     AKRFILFHKKRHPQDMGRPEIEAFLTHLAVQGKVAASTQNQALNAIVFL"
BASE COUNT         4052 a         6308 c         7360 g         3663 t
ORIGIN      
        1 gggggcaaaa ggacgttgtt cttgtaatcg acggcgcaac tcggtataat acaccggttt
       61 ctcctgggac agtattttag tttttagtcg taaattcaaa cagatcggag agtgagacgg
      121 tgaaagctga tatccatccc acctaccaag aggccctgat tacctgtgcg tgcgggagtg
      181 tgttgaagac acgatcaacg gtcaaggaca tccgtgtgga aatctgcgcc atgtgccatc
      241 cgttctttac ggggatgcag aagatcgttg ataccgaggg acgtgtcgag cgcttccgta
      301 aaaaatacag caataacaag aagtaacgca cgctattctc gatcgctgct ggcggtgacg
      361 gggcgcgagc cgtgccactc cgagcacaat aatattcgtg tcgttcgtga ctgaacaggc
      421 tgatccgatc atcctgcgtc tggagacgct ggcttcgcgg tttgccgagg ttgagcgcaa
      481 cttgggggac ccctcggtga tcgccgatcc ccaggcgctc cagcgcctgt ccaaggagcg
      541 cgcggaactc gccgacgtgg tcgggcaata ccaggcgtat tgcgcgctgg tgaccgagtt
      601 ggaacagacg cgacggctgg ccgaggatcc gcgttccgaa cccacgttcc gggaactggc
      661 gctggacgag gctcagacgc tcgaggtcaa aagggccgag ctgtacgacg cattgcgcat
      721 cgccctgctt ccgaaagacc ctcgggacga gaagaacatc tttttggaaa tccgcgccgg
      781 aaccggcggc agcgaggccg cgttattcgc ggccgagttg tttcgcatgt acggcaaata
      841 cgcggaaacc aaacgctggc gggtggagtt ggtgtcgtcg agcgagaccg gcatcggcgg
      901 gatgaaggag gcgatcgtcc tcatcgaagg caaaggcgcc tacagccggc tcaagttcga
      961 gagcggggtg caccgggtgc agcgggtgcc ggtgaccgaa gcgagcggac gcattcacac
     1021 ctccacggtg acggtcgcgg tggttccgga ggccgaagaa gtcgaatacc acatcgatgc
     1081 caaggatctg cgcatcgaca cgttttgttc ctcggggccc ggcgggcaga gcgtcaacac
     1141 cacgtactcc gcggtccgca tcacgcacat tccgaccaac acggtggtga gttgccagga
     1201 cgagcgctcg caaaccaaaa atcgcgccaa ggccatgcgc atcctgcgct cgcggttggt
     1261 ggaggtggaa cgggagcgcc aggaggcgga gatcgccaag gaccgcaagg cccaggtggg
     1321 cacgggcgag cgcagcgaaa aggttcgcac ctacaacttt ccccagaatc gcgttacgga
     1381 ccatcggatc gggctcacgt tgcatcggtt ggaccaggtg ctcaacggcg acctggacga
     1441 gttgatcgac gcgctccagg catggacgca gaccgcgcaa ctgcaacgac tctgagcccg
     1501 ctcacgcgcg gggccgcggt gcaatgggcg agcggcctgc tcgcggccgc gggcgttgaa
     1561 tcgccgcgag tggaagccga cgcgctcttg tccgaggcgg tgcatgctcc ccgctacgcg
     1621 tcgtatctgg agccggacgc gaccttggcc gagagcgcgc acgagcgatt cgtcgcgatg
     1681 gtcgctcggc gcgcgcaacg cgaaccgatc caatacgtga ccgggcgtga gacgttctgc
     1741 gggttggatc tggtcgtgac gcccgacgtg ttgattccgc gtccggagac cgagggcgtg
     1801 gtggccgcgg cgctcgatgc catcggggat cggggccgac ccgtggtcgc ggacgtgggc
     1861 accggcagcg gggcgattgc attggccgtg gccgcggcgc gacccgacgc cgtggtgtac
     1921 gcggcggatc gttccgcgcg cgcgttggac gtggcgcgcc gcaatgccga aagactcgca
     1981 ttgtcggacc gcgtgcagtg cgtggaaggt gactggttcg agccgctcat cgagcgcggc
     2041 gtgcgggcgg acgtgctggt gtcgaacccg ccgtacgtgg cggacgcgga gtacgcgcgg
     2101 ttgcaggccg aggtgcgctt cgagcccgcg caggccttgc tcggcggacc cgacggcttg
     2161 gcgttctatc gacggatcgc ggcggaatcg cggcacgtgc tcgcgcccgg cggtaggatc
     2221 gtgatcgagt tgggcttcgg gcaggccgac gcggttcgaa ccatcgcgga acaggcgggg
     2281 ttcgcaatcg aacgattgga cgacgacgcg aacggcatcg cgcgcgtgat gacctgggcg
     2341 gaggcgacgt ggacctgatc gcggtgcagg gcggccggcg actcgtcgga gaaatcccga
     2401 tcagcggggc caagaacgcg gcgctgccgt tgctcgcgtc cgcgttgctc agccaagagg
     2461 agtgcgtgtt gaccaacgtg ccgaacctgc gggacgtgaa caccacgctc aaactcctgc
     2521 gccacctggg cgcgtccgcg caggcggacg gcgcgggccg cgtgagcgtg acggccggga
     2581 ccgtgtcatc cgccgacgcg ccgtacgagt tggtcaagac catgcgcgcg tcgattctgg
     2641 tgttggggcc gttgatcgcg cgctgcggcg aggcgcgcgt gtcgttgccg ggagggtgcg
     2701 cgatcggcgc gcggccggtg aatctccatc tgagcgggct ggagcggatg ggcgccgagg
     2761 tctcgatcga acacggctac atccgggtca aggccggaac attgcgcggc gcgcgcgtgg
     2821 cattggatct gcccaccgtg accggtaccg agaatttgct gatggccgcc acgttggccg
     2881 aggggaccac cacgatcgaa cacgcggcgc gcgagcccga gatcatcgac ctggcgaacg
     2941 cgttgatcgc gcgcggggcg aagatccagg gcgcgggcac cgacacgatc acggtggacg
     3001 gcgtggcgtc gctgcgcggc gggcagtatc gcgtgatgcc ggatcggatc gagaccggca
     3061 cgtatctggc ggcggccgcg atcacgggcg gggacgtgtt cctgcgcggc ggggtcgggt
     3121 cgcacctgga agccgtgctc gccaaactcc gcgaggcggg cgtcgaggtc agggccgagg
     3181 cggacggcct gcgcgtgacg cgtcgcgggt cgttgcgcgg ggtggatgtg accaccgcgc
     3241 cgtatccggg atttcccacc gacatgcagg cgcagatgat ggcgctgctg agcgtggcgg
     3301 acggcgcgag cgtgttgacc gaaaccgtgt tcgagaatcg catgacgcac gtcgcggaac
     3361 tgcagcgcat gggcgcctcc atccgcgtgg agggcaacca cgccgtggtg cgcggggtgc
     3421 cgcgcttgag cggagcgccg gtgatggcgt cggacctgcg cgccagcgcg tgcctggtgg
     3481 tggcgggtct ggccgcggag gacgagacca tgatttcgcg cgtgtatcac ctcgaccgcg
     3541 ggtacgagcg catggaggag aagctgacgc gggtcggcgc cgtgattcga cgggttcgtg
     3601 aggcggcgtg agcgacgcga agctggtgat cgcgctcccc aagggacgac tgttggagcc
     3661 caccgtcgag ctcttcacca ggatcggcat tcgtcccaag gggctgacgc gcgacacgcg
     3721 ccaactgctg ttccgaaccg accgggaggg cgtgtcggtg atggtcgtgc gggcggtgga
     3781 cgtgccgacc tacgtggaat acggcgccgc ggatctcggc gtggcgggca aggacgtgtt
     3841 gctcgaacac ccgaaggacg tgtacgaacc ggtggatctg ggctacggcc tgtgccgcgt
     3901 ggtgtgggcc gcgcccgcgg gccgtccggg aacgtgggcg ggctctcgcg cggggaaact
     3961 gcgggtggcg accaaatacc cgaacatcgc ggatcggtat ttcacgcagc ggggcgtgcc
     4021 gatcgagatg gtcaaattgt ggggcgcggt ggaactggcg ccgcgcatcg ggttggcgga
     4081 gagcatcgtg gacctggtgt ccaccgggaa aaccttggaa gaaaacgggc tgaccatcgt
     4141 cgaggagatc gtgcggtcca ccgcgcggtt gatcgtcaat cgggccagca tgaaggttaa
     4201 gcactccaga atcggtgaac tcgtcgaacg ggtgacggcg gtctgcccgt ctccgacgtg
     4261 acgcccggca acgggtagag aggacgacgt atggcgatga aggtgatcaa actcaagggc
     4321 agcgcggggc gtcgcgagct cgacaagatc gtcaaccgcg gcgagcccga cgatcgtcgg
     4381 gtcgaaacga aagtgcgggc gatgctggac gacgtgcgcg ccaagggcga cgtcgcggtc
     4441 tcccggtaca ccaagcagtt cgaccacatc gcgatgtcgc cgaagcgctt tctggtgacc
     4501 atcgaggaac gggcggccgc gttggccaag accgatccca aggtggtgga agcgctcaag
     4561 ctcgccgccg accggatcta cgcgtttcat tccaagcagc agtcgcattc gtggagcttc
     4621 gaggaggcgg gcatcacgct cggccagcag ctccggcccc tggcttcggc cggactgtat
     4681 gttccgggcg gcaaggccgc gtacccgtcc tcggtgttga tgaacgggat tccggccaag
     4741 gtcgccgggg tcgagcggat cgtgatgtgc acgcccacgc cgcgcggcga gatgaacccg
     4801 tacgtgttgg tcgcggcgga cctggtcggc atccacgagg tgtatcggat cggcggagtg
     4861 caggcgatcg gcgccatggc ctacggcacc aaaacgatca aacgcgtgga caagatcgtg
     4921 gggccgggga acgcctacgt ggccaccgcc aaacgtctgg tgtacggcac cgtggacatc
     4981 gacatggtgg cggggccgag cgagatcctg atcatcgcgg acgagaccgc ccacccggcg
     5041 cacatcgcgg cggatatttt gtcacaggcc gaacacgacg aggacgcctg gaccatcttg
     5101 atcgcgctga gcgacggatt cgtcaagaag gtgcagaaag agatcacgtt gcagttggcc
     5161 aagctcgatc ggcgcaagat cgccgaggcc tcgctcaaac accgcggggt cgccatggtg
     5221 gtgcccgaag tggtggacgc gatcggcatc gccaacgaga tcgcgcccga acacctggaa
     5281 ttgtcgatcg agcggcccta caaggtgctc aagttcgtca aaaacgcggg ctcgattttc
     5341 ctcggacacc acacgccgca ggccttgggc gattatctcg ccgggcccaa tcacgtgttg
     5401 ccgaccggcg gcacggcgcg gttcttctcc ccgctgtcgg tggatgactt caccaagaag
     5461 agcgggctca tcgcgttttc gcaagacgcg ttgcggcgct tttcggaaaa gctggtgcaa
     5521 atcgcgcgcg tggaagggtt ggaagcgcac gcgcaggcgg ttgaaattcg catgcggccg
     5581 gaggactgag catgccgggt cgtgccgcca cgcgacgcgc gtccggttca tcgcgacggc
     5641 ttccagcgcg acgcgccgcg atctcgcgca agaccaagga aaccgcgatc gtcgtgaccc
     5701 tcgatctcga cgggaccggt cgcacgcggg tggcgaccgc gatgccgttt ttggatcaca
     5761 tgctcacggt catggggaaa cacgggttgt tggacctgac cgtgaaggcg tccggtgatc
     5821 tggacgtgga cgctcaccac acggttgagg atctgggcat cgtgctgggg caggcgctca
     5881 aacaggcgtt gggcgacaag gccgggatca cacgtttcgg cgccggagcc gtgcccatgg
     5941 acgagacgct cgcgcgcgcg accgtggacg tgtcgggacg gccgtatctg gtgtaccgcg
     6001 tgcccgtgac cacgcgcaaa attcagtcct tcgacaccga gctggtggag cactttttcg
     6061 aggcgctggt ggtgcacgcg ggcctcaccc tgcacctgga agtcccgtac gggaagaacg
     6121 cgcaccacat gctggaggcg accttcaaag cattcggccg cgcgttggaa caggcgacgc
     6181 ggatcgatcc ccgcgtagcg ggcgtgccgt cgagcaaggg gcggatcgaa taaaacaaag
     6241 acgttcaact aaaacaaaga cgttcaacgt tcaagggttc aacgttcaat atgaacccat
     6301 gaatttttga acccttgaac ccttgaaccc ttgaaccctt gaacctcttt gccgtttgaa
     6361 ccatgatcgc cgtcatcgat tatcaagtcg tcaatctgcg cagcatccag aaggcgttcg
     6421 aacgcctcgg tgtggaggtc gaggtcacca gcgatccggc gcgcattcgg cgtgccgagc
     6481 gcgtggtgct cccgggggtg ggggcgtttc gggccgggat ggatcagctc gcgcggttgg
     6541 gcctcgtgcc ggtcattcgc gacgtcatcg acgccggcac gccgttcctg ggaatctgtc
     6601 tggggatgca gttgctcatg acccagagcg aggagttcgg cgtgcatcgc ggattacacg
     6661 tgatcccggg caaggtcgtg cgtttcccca gcgagacgcc cgcgatgaag gggctgaaga
     6721 tcccgcacat ggggtggaac gcggtcgtga tgcgtcgacg tccggacgca ttggacggca
     6781 tgccggatcg tgggtatttc tacttcgtgc attcctatta cgtggtgccg gacgatccgg
     6841 cggtgatcgc cgcggacacg acctacgggg tgcgattccc gtcgatcctg gcgtggcgga
     6901 acgtggtggc gtgccagttt cacccggaga agagccaggc gttggggctg tcgctgctcc
     6961 gtcggttcgc ctcgttccga cccgtcgcca cgggggccgc gttgtgatca tcatcccggc
     7021 gatcgatatt cgcggcggcc ggtgcgtccg gttgttgcag gggcggttcg acgcggagac
     7081 cgtgtacgga gacaatcccg cggccatggc cgagcggtgg caggtcgagg gcgcggttcg
     7141 attgcacgtg gtggacctgg acggcgcggc cgacggggtc ccgaagaacc gcgacgcgat
     7201 cgcggcggtc gtgaaggccg cgcgaatccc ggtccaggtg ggcgggggcg tgcgcgacgt
     7261 gaccaccatc gacgaatatt tgacgtgggg cgtcgaccga gtggtgttgg gtacgcgcgc
     7321 cgcgctcgac cccgcgttcc tgcgcgacgt gtgtaaacgg tttcccggga aggtcgtggt
     7381 ggccatcgac gcgcgcgacg ggcgggtggc gatccgcggt tggctggaag ccacggaccg
     7441 cgacgcgctg gacgtggcgc gggaagcctc ggcggccgga gccgcctcga tactgtacac
     7501 cgacatcaaa cgggacggga cgcaggaggg cccgaacctc gaggcgctcg ccgcggtcgc
     7561 ggccgcgacg ccaaccccgg tgattgcgtc gggcggcgtg tccacgccgg agcacgtgcg
     7621 gtcgctggcg cgggtcaagg gcgtggaggc cgcgatcgtg ggtcaggcgc tctatcgcgg
     7681 gacgttgtcg ctgcgcgacg cgatggcggc cacggagagc agccggtgat ggccgactgg
     7741 ccgccgaaaa cgggtggata cccccccttc tgggccttcc ggcgttttct tctccgggtg
     7801 ggtcgcgagg cggaacctgc gccgcgccga cggcgcgaaa tggaaatcgt ccattccgct
     7861 caggtggcgc agcaacgaaa ccgcgccccg cagcgtactg gggcgtaggt gaggaggcga
     7921 ggctgacgtg acaacgcagc aggcgcccgt ctccggcgga tcagtcacgt tcgccaaacg
     7981 cattattccc tgcctcgatg tgaaggacgg ccgcgtggtc aagggcgtgc ggttcgtgga
     8041 tctgcgcgac accggcgatc ccgtggaagc cgcgacgtcc tacgacggcg agggcgcgga
     8101 tgaactgtgt tttttggaca ttaccgcgtc gtccgacgag cgcccgatcc tgctggacgt
     8161 ggtgcgccgc accgcggagc gcgcgttcat gcccctgacc gtgggcggcg gggtgcggag
     8221 cgtcgaggac atccgggcgt tgttgctggc cggcgccgac aaggtttcga tcaacagcgc
     8281 ggcggtggct cggccggatc tgctccgtga atccgccgag cggttcggca gccagtgcat
     8341 cgtgatcgcg atcgacgcgc gacgcggcga acgcgggtgg gaggtgctca cgcacggcgg
     8401 gcgacgcgcc gctgaattgg acgcggtcga gtgggcggtg cgagccacca cgttgggcgc
     8461 gggcgaggtg ttgttgacca gcatcgaccg cgacggcacg cagcaggggt acgacctgga
     8521 gttgctgcgc gcggtctcgt ctgcggtgcc cgtgccggtc gtggcgtccg gcggcgcggg
     8581 cacgctcgaa catttttacg acgcgctcac gaccggaggg gcggatgcgg tccttgccgc
     8641 gtcgctgttt catgatcggc ggttgaccgt cgggcaagtg aagcagtatc tggcggaacg
     8701 gggtgttgtt gtccgaccgg tgtgagtgag cctgttcagc gagggccgtc tgcggcgttg
     8761 ccgctacggg gccgcctcct cacgtacgac ccagtacgct gcggggcggt cccttgcggc
     8821 gccgtgcatt caagcccgtc ggtgaacagg cttatcagaa gcggaagtca gaaccagccg
     8881 ggtggctggt gaggtgcggg cgtggcgaag aagaaagaag tcgatagtct ctggagcgcg
     8941 atcaagtttg acgcggcggg gttgattccg gcggtcgtgc aggagcgcgg atcggggcgc
     9001 gtgttcatgc tcgcgtacat gaaccgcgag tccctcgagt tgacgctgaa gacggggttc
     9061 acgcactttt tcagccggtc gcggcaggcg ttgtggaaga agggggagac ctcggggcat
     9121 acgcagcgcg tggagtccgt tcggctcgac tgcgacgggg acactctaat cgtggaagtc
     9181 gagcagaccg ggccggcgtg tcacaccgga gaaccgacct gtttcttccg gctcgttgac
     9241 ggcgaacagc tgacgccgga ccctgatggg ggcggcgcgg tgttggatcg cgtgtatcgg
     9301 gtcatccagg accgcaaggc gcatcccgtg gagggctcgt acgtcgcgtc gttgttggcg
     9361 ggcggggtgg accggatcct caagaaggtg gcggaggaag ccggtgaagt tgtgctcgcg
     9421 tccaaaaacg gggcgcgcga tcagatcgtg tgggaggtgg cggacctctg gtttcacacc
     9481 ctggtcgcgt tgggacacca cgacatccca ccttcggcca tctacgacga attgactcga
     9541 cggtttacgc accgcgcgcc cgcgccacgc aagcggcccg caaccaaacc gaaacgcgcg
     9601 ctgagaaagc ccgcgcgctg accgcgcggg tccgcatcga ttagccgagg aggtgggatg
     9661 agcgcatcaa attgcctctt ctgtcgcatt gtccgccggg aactgccggc gaccatcgtg
     9721 catgaagacg acgaggtggt ggcgtttcag gacattgcgc ccaaggcgcc ggtgcatttc
     9781 ctcgtgattc cgaaacggca tctcgacggc ttgcaggcgg tggacgcggg caacgcgtcg
     9841 atcatggcgc cgatcttcgc gtgcatcggt cgcctggccg tgcaattcgg cgtcgcgaaa
     9901 tccggctatc gtacggtgat caacgcgggg gcggacggcg gccagaccgt cttccacctg
     9961 cacgtgcacg tgctgggcgg ccggcccatg acgtggccgc cgggttgagc ctcgcgtcgg
    10021 gttgacccct ttctcgacca ttccgtataa tacgaccctt tccacaccct tcaccgaaac
    10081 gggaataatg gccctgcagg aacggttggt cgaagagatg aaagaggcca tgcggagcgg
    10141 gcaggccgcc aagttgtcgg tcatccggat ggtgaggtcc gcgttgaaaa accgcgagat
    10201 cgatcgcggc aaggacaagc cactgaccga tcaggacgtc ctcgacgtga tccagagcgg
    10261 catcaagcaa cgccgcgaag ccatcgagca atatgaaaaa ggcggacggg ctgagttggt
    10321 cgccaaggag aacgaggaga tcgcgattct ccagacgttt ctcccggcgc aactgtccgg
    10381 gccggagctt gacgcattgg tgaaggcagc ggccgccgaa gtcggagcgt cgagcatgaa
    10441 ggacatgggc aaggtgatga agtccgtgat ggcccgcgtc gcgggtcgag ccgaaggcgg
    10501 cgcggtcagc gcggcggtga aacgaacgtt ggagcgtctc ccaccaccgt agatggcgtc
    10561 aatggctccc gatcgccccg ccaccgagcg gccgggtcag tcccccggag caacccgtca
    10621 cgcaggcggg ttcggcgacg cggagttgcg cgcgatccgc gagcgcgtgg acttggtcga
    10681 attcatcggc gcctacgtct cgctcaaacg caccggacag aacgcggtcg ggctctgtcc
    10741 ctttcactcg gacaaacgtc cctcgttcac ggtcagcgcc tccaagcagt tgtaccactg
    10801 tttcgggtgc ggggccggcg gcgatctctt cgcgttcgtg atgcagcgcg agtcgttgag
    10861 ttttcccgag gccgtgcagt ggctggctcg caagagcggg gtgccgttgc cggcccacga
    10921 gcgctccgac gaggaccgcc gccgtcagtc gctgtacgac atccacgcgg ccgcggccga
    10981 ccattttcgc cggcgactcg ccggacgcga gggcgaaacc gcgcggcggt atctccacgg
    11041 acggcggttg accgcggaca cggtggaggc gttcgggctg ggctacgcgg cctcgtcgtg
    11101 ggacgatctg ctcgccgcgt tgagcgcgaa aggctggacc tccgcccaag tgcaggatgc
    11161 ggggttgatc gttccccggc agcaaggcgg cggctggtac gaccgctttc ggcatcgggt
    11221 catctgcccg atccgcgacg cgcacggcca ggtcgtggcg ttcgggggcc gggtgctgga
    11281 cgacgcgcaa cccaagtatc tcaattcccc cgagaccccg ctcttcagca aaggccggca
    11341 actcttcgcg ctggaccgcg cgcgccggag cgtggcggcg aacggcaccc tggtggtcgt
    11401 ggaaggctac ttcgacgtga tcgcggcgca tcaggcgggg tttaccaacg tggtcgcgac
    11461 cctggggacc gcgctgacgc ccgcgcactt ggaggcgatc cggcggctgg tcaaccgcgt
    11521 ctgtctggtc ttcgatccgg accccgcggg ggtcaaggcc gcgttgcgga cggtggatct
    11581 cttcgcgccg ctggacctcc gcgtcgacgt ggcgtcgctg ccgggcggca aggatccgga
    11641 ccgcgtgatc catgaagacg gtcccgactc gttccgcgcg gcattggaac aggcggtggg
    11701 gctgacgcaa ttcgtcctgg gtcatttggt tccggaagga tcgtaccgcg atcccggtgc
    11761 gaagaaggcg gcggtcgcgc gggcatcgcc cctgatcgcc gccatgacca acgcgatcga
    11821 tcgtgaccac tacctccgat ggctggccga ccgagtgggc gtggcggagt ccaccctgcg
    11881 cgaagagctg cggcgcggcc gcaatcccgc cgcgccccgt accgttgccg cgacggtcac
    11941 accgcgcggc gacgccggga aaccggttgc ggggtggccg cgggcggaag aggtggtcgc
    12001 cgcggccgtg attcacggcc gtctggcccc ggcgctgatg gcggacgtct cgggcgacgc
    12061 gatgaccgac cccaccctgc gggccgtggt ggagttggtg gagcgggaga cccggtccgg
    12121 cgacggcgcc gcggccgtga tcgcgcgcga ccgagccctg gcggaaggcc gtcgcgacgt
    12181 ggccgaggcc ctcgcgaaac tggcgacgta cgagttcctc ggagatgacc ccgatcagga
    12241 gattttcgat tgcgtcgtca ccctgcgcat tgatcagcgc agacgccgcg tcgaggaact
    12301 cacgcgggcg attcaccgcc acgaggcgca gggcaagaac gacgaggcgc aacgactcca
    12361 gggtgaacat gttcagctga cgaaggaact gagcgggttg cgattgttgc ggggttcgcg
    12421 tatggggccg gcgcaggcgg ccggtcggta gggtaccagg ggtgtccatg tctaaagacg
    12481 agaaactcga cgaagtcaaa aacctcatct cgttggggaa ggagaaggga ttcctgacct
    12541 acgacgaact gaacaacgcg cttccggcgg acgtcctgtc ctccgagcag atcgacaacc
    12601 tgatggtcat gttcggcgaa atggacatcg aggtcgtcga ttccggggag gagagccgct
    12661 ttccccagat ggaggaagcg gaagaggagc tggacgaact cgaagaaggc gaggaggtcg
    12721 gcgaggagtt cctggaggag agcgaggaga aggaaaagga aatcgacctc acgcccggca
    12781 tcttgagccg caccgatgat ccggttcgcc tctatttaaa ggagatcggc agcgtccatc
    12841 tgttgagccg cgagggcgag atcgaaatcg cgaagcggat cgaggagggc aaacgcgaag
    12901 tcagttcgat cgtgttcggc atgccgatga cggtcaaaga agttctggcg agcggcgaac
    12961 tcctcaaaca gggcgtgatc gcggtcgagg atctcgtggt cgcgttcgac gacgatctgg
    13021 gcgagcagga agagggcccg cgcgatacca cggaaatcaa ggccaaagtg ctgcagatga
    13081 tgggcggaat ccgcgcggcg tgtcgcgacc tcaaccacgt cgcgatccga tggaagcgga
    13141 ccggcggcga ccccgccaaa cgcaagaaga tcaagccggc ggtcaaggag gccaaggacc
    13201 gcctggtgga gaagatggag gcgctgaacc ttcagccgcg ggtcctggac gaagtggtca
    13261 aacgtctcaa gaccctggcc gacgacatcg aacggagcga acgcgagatg gcggacgtgc
    13321 gtcgcgcgct gggtcatccc gacgaggagc cgttgttgat ctttcgccgg atgcggcaca
    13381 acccgcgcgc gctcaaggcg gcgctcaaga aaaccgaact gagcgaggaa tcgttcacgc
    13441 aagccgagaa gcgctaccgc aacgcgctgc ggctgatccg gaccgcggaa atggaggcgg
    13501 ccgtgtccgc acccgaattg aaggacgcga ttcgccaact ctatttgggc gatctgagga
    13561 tcaaacgcgc caaaggggag ttggtggagg ccaatctccg attggtcgtg agcatcgcga
    13621 agaagtacac caaccgagga ctgcagttcc tggatttgat ccaggaaggt aatatcggcc
    13681 tcatgaaggc cgtggacaag ttcgaatacc ggcgcggata caagttttcg acctacgcca
    13741 cgtggtggat ccggcaggca atcacgcggg cgatcgcgga ccaggcacgg acgatccgca
    13801 tccccgtgca catgatcgag accatcaaca aactgatccg gacgtcccgg ttgttggtgc
    13861 aggaactcgg gcgcgagccc ctgccggaag aaatcgcgga gaagatggac ctttcgctgg
    13921 acaaggtccg gaagattctc aagatcgcca aggagccgat ttcgctcgaa accccgatcg
    13981 gcgaggaaga ggacagccat ctcggcgact tcatcgaaga caaacgggtc gcctcgccgc
    14041 tcgaagccac catccggtac gatctgcagc gtcagatcgg caaggtgctc ggcacgctga
    14101 ccaccaggga agaaaaggtg ttgcggaagc gtttcgggat cggggagacg accgaccaca
    14161 ccctcgaaga ggtgggacag gattttgagg tcacgcgcga gcggatcagg cagatcgagg
    14221 ccaaggcgtt gcgcaaactg cggcacccga gccggagtcg gcgactgaag agtttcgtgg
    14281 aagggtagtg aactatcaag agccaggaac tccgagcctc ggcgcgggat aggtcaaagg
    14341 gcagaataca tcgctcctcg gcgcgggaag tcggcgcctg tggggcgctc cgcaagcgcg
    14401 gtggggccca tagctcagtt ggtcagagct gccggctcat aaccggttgg tcccaggttc
    14461 gagtcctggt gggcccatcc tcgctagcgg agcgtagcgc aggccctggt ttgcttcgcc
    14521 acctgggagg aaggggagac ggtttaggac ctgccccttc ggggcagtgc aggttcccgg
    14581 gccgagcagc gaggtcagtg gtcttggtgg tgatgtggcg gttgaatctc tcatgaagga
    14641 tatggtgtga acgaacaact cgctctgttg atccgccttc aggaattgga ctcccaaatc
    14701 gacgcgttgg ccgagcaacg tcggcaggcg ccagcgctcg tggaggcggc ggccaagccg
    14761 gtgcaggagg cggaggccga actcgtcaag acgaaggcgg cgttcgacgt gttgacccgc
    14821 gatcgtcgcg acaaggaaca ggaactcaag gagcgtgagg atcggatcga gaagctcaag
    14881 ggccgttcag gagacatcaa atcgaacaag gagtatcagg cccacctgtt cgagatcgaa
    14941 ctcgcccaac aagaaaaggg cacggtcgaa gaacgcttgc tcgagatgat ggaccgtatc
    15001 gaggcgatgc aggcgaccgt caaatcccag gaggccgcgg ttgcggtctg caaacgcgag
    15061 ttcgacaagg aacgcgcccg cttggagggc gagcagcggg cggcgcaggc cgaagccgac
    15121 cggttggttg ccgacgccaa gtctctggcg gaacagctcg cgccggccaa ccgccaggcg
    15181 tatgagcgcc tcaagaccac gcgcaaaggc ttggcggttg tggcggtgaa gaatggggcg
    15241 tgccagggct gccgcctcgc cctacctccg caactgatcg ccgatctacg caagaacgag
    15301 aaattgttga cctgcaccta ttgccaccgc atgctgttct ggccggtttc ggatctggcc
    15361 gcgcaaaaaa cctgacatcg gatccgcaac gctcgcgggc acgtgcgacc cgtgggcgcc
    15421 gtcccgcgat tgagagtttg gtcggccctt gttagaatgc gcgaatatga cgcccgtctc
    15481 tcgtccatcc cgcgcgaccg ccaaagccct gcgcgaaacc aaccgcgagt tggacgtgct
    15541 gcgccggatc agcgaaacga tcagttgcac gctcgaccag gaggcggtgc tccggcagat
    15601 catcgacctg gtcgtcgaag tgacgtcgtg cgacgcggcg tttctctacc tcgtggacga
    15661 ggggggcaag gagctggtgc tgcgcgcgtc caaacagccg cacccgcgtt tgatcggccg
    15721 gatcaaattg gaaatggggg agggcatcac cgggtgggtc gctcgcgagc gcactccggt
    15781 ggccatcgcc cggcacgcga gcgacgatcc gcgcttcaag tacttccaga aattgccgga
    15841 agaccggtac caggcgtttc tctcggttcc cgtgatcgcg aagaacgagg tcatcggggt
    15901 catcaacgtc caacaccggg agccccatcg gcataccgcg tcggaaatcg ccctgttgtc
    15961 cgcgatcggc caccaggtcg gcggcgcgat cgagaacgcc agcctgtacg acgcgatgcg
    16021 tcgcaagacg ctgcaactcg aaaccctgct ccaggtcagc accacgatca cctccacgcg
    16081 gtatctcgag gaaatgctgc ccctgatcgt caccatcacg gcgcggacca tggggtcggc
    16141 gatttgttcg atcatgctga ccgacgagca gcgcggcgag ctgcggatcg cggcgacgca
    16201 gagcttgtcg gacgcctacc gcggcaagcc gtcgctcaag atcggggaag gcgtgagcgg
    16261 gcgggcggtt cgggacaaac gtccgatcgc ggtgaccgac gtcacggagg acccggcgta
    16321 cgtgtatcgc gagttggcgc gccgggaagg cctgcggtca ttgttgtcgg tgccgatgct
    16381 ctataaggat cgggcggtgg gggtgatcaa cagctacacg gcggagccgc acgtgttccg
    16441 gcccgacgag atcgaactgt tggaagcggt cgcgaatcag gcggcggtgg cgatcgagaa
    16501 cacccggctc atgcaggaga cggcccagat gcacgacgcg ctggagctcc gcaagctggt
    16561 cgagaaagcc aagggcatct tgatgcgcga gcgccggttg accgaagaag aagcgttccg
    16621 agttctccag cgccagagta tgaacaaccg atggccgatg aagcaggtgg ctgaggccgt
    16681 catcggcacg gcccgcggcc gcacgtaggg gaggctgctg aaaaagtccg gctgcggcgt
    16741 tgccgccgcg catccggtgc tcacgtacga ctcagtacgt tccgctccgg tgctcccggc
    16801 gccttgcatc cggagctttt tgagcagcct ccagaggcaa agcgtttttc aggaccctga
    16861 gacgtctgaa ttcgcgcaag ggcgaggccg gggggcgcgg tttcccgcct cggggctacg
    16921 cggacggggc ggcggttttt tctttcttgg gtttggcttt gacggcgggt ttcttggcgg
    16981 cctgagacgc ggatttcgcc ttgagagttt tcacgcgctc gcgcagccgt tcgcggcgcc
    17041 gcttctgacg tcgccgtaat tctttcgctc gttctcgtcc tggtgccata ccgtctcctt
    17101 gtcactaaac gccgaccaat tcgtccaccg agagtagggg ccattgtaag gaaacgtgag
    17161 tgaaagtcaa ggatgcggcc gcgcggacaa tcgtgcgggg aaccgactcg tcatcttgac
    17221 acccctggaa cgcgcggcgt attgttctcg ttgaaacgag atttcacatg acgccgcgat
    17281 caaaaaccaa gacccgttcg cgggaattgc tccatgaacg gctccgtgaa cacgggttga
    17341 agttcacgca ccaacgcgag ttgatcgcgg agctgtttct ggaacaggat cacattacgg
    17401 tcgatgaatt attccagaag gtgtccaaac gggacccgtc gatcggcgtc atcaccgtgt
    17461 atcggaccct gaagttgctc tgtcaatggg aactcgcgca ggagcggcac ttcggcacgg
    17521 gcaagacgat ctacgacaac gtggcgtgga agtcccacca cgaccatctc atttgcaccc
    17581 actgcggtcg catcatcgag ttcgagaact tccagatcga gcagttccag gagcaggtcg
    17641 cgcgccaaca cgatttccga atcaccttcc ataagttgga attgtacggc atctgcaagg
    17701 cctgcgagtc cgaggagcgc gccaaaggtc gggcgacccg ctccgtgccc caccatcaca
    17761 gacattaaat ttcgtgcttg acacgccgcg gggagttccg atatattcac gtgcgtatca
    17821 aatgaaaatg aattccattt gcataaatga cggtcggtgc gcggcgttag ggtcatgagg
    17881 ggcgattgac gagggggtga cgcgatcgcg ggagcgtcgg cttccgggat ggcggcagca
    17941 acaggttagc ggctcgaggc gaacagtcca aagcccggaa tgaattcatt ccgggctttt
    18001 ttttctcgcg gcttcgggga gatccgacaa ggccaacagg gggatcgtca tgcggcacta
    18061 cacacaacgg cggttcaaaa ccaagcgggc ggtgaggcga aacatgaata tccaacacga
    18121 tcaaaaaggc gccgtctcag cggaggatct tgatcagaaa ccgccgtgcc gcagcgagtc
    18181 gctggtgtcg aatgtggacg ggaccgtgac caagtgcggg tgcgggcact accacgtgcg
    18241 gatcaacgcg gtgacgttgc acctgaccgc gctccaattc gacgcggcgg cccggttatt
    18301 caagttggcg tggggcatgg tggtggggcg ccggatatcc gtgtcgatgt cccggttgac
    18361 gtgcgtgccg gtgctcgcgt tgctcgccgt cgtcggatgg atggggctct ccttcgcgtc
    18421 aaccccggga tttttggtgg tcgcgccgga tcgcggattt gtcggcaatc aagaaacgtc
    18481 cgcggtgttc gacgccttca atgcgtccta ccctgcagcg ctggcgttcg gcggacggga
    18541 ctacagcgat cgtcccggaa actactcagg gtatctgacc gcggcggttg agcgactgaa
    18601 ggccgcgggg gcgaccgaga tcgtggcgat ccctctgttc ctgtcgtcgg ccgatccggt
    18661 gttgaaaacc gtccagtcca cactcgcgag ccgcgcggga cagatcccgg tgcgctggac
    18721 cccctcgatg caggacagcc atctgaccgc tcagatcttg gtggaccggg tggtgtcgtt
    18781 gagccgagac cccgaggcgg aacgcgtgat cctgctcggc accggcgcgg ccgacgcggc
    18841 ggccgaagaa aagctccggg cagatctgga ccgctcacag gccttagcga tgcgcgcggc
    18901 ccacgcgacg gcggcctcgc gcgagggcag ctcgagcacg gtgaagccgc cgttgagcgg
    18961 gggcgcccac gggtagccgc cctcggcgac cgtgccgttg gccgagacga gcgcgggcgg
    19021 cacgctttcg tctaggccgc cgccgaagac gtagacgccg gcggcctttg cctcgccaat
    19081 cacggcgtgc gcatcgcggt ccaccacctc ccactcgtcg tcgggcacga ccatcgcggc
    19141 gctggggaag gaaatcaggt acttggccat ggtactccct ctttgatggt tgacgacgat
    19201 cgtgaagcgt gtgtgcggcc taacgcgaaa actaacccgc cggactccga cgaaacgagc
    19261 ggcagcgagc cgccatgcta agcccggtcg ggttgagtaa cgggttaggc tcaggcagat
    19321 ttctgatttg ctcgaatgtc attgagggtt gcataggcgc tcacaaagcg gatggcgtcc
    19381 atgggcgaat acaaagaaac aactgcttga ctctgtttcc cgtcgctgtc gatgaagtag
    19441 ccgacgaaat ggtcagaata ccagcgaaac aaaaattcgg gagcgtggtg tttcttgtga
    19501 atgactcgca ctgttccact ggtgctccct gatacctcaa cagagattcc atcgagttct
    19561 tctagggact tgagccggtt cacaattgtc tgttgggaca tgtcgcctcc gttttgatag
    19621 gagaaccgcc taacgcctaa actaacccgc cggccaccga tacaacgagc gaagcgagtg
    19681 tgccaagctg gcacccggtc gggttgagta aattgttagg gcctccgacg gctaatcacg
    19741 accttatgac gaagtaacaa gaaagcgaca agaagcattg tgacgccagc caccccccat
    19801 agccagaagc cttccatgaa gcgagaaacg gcggtaaagc cagcgcttgg aatggcgtcg
    19861 ggacccatcc cgtctcgaaa taagagactg aaaaagccac ccaccagaag tgcgagtaga
    19921 ccaaaagcgg cacagacaac tccaacaacg gtgccaatta tcccaacaat agaacgggcc
    19981 ccgaaagtca ttggttgcgc catagtcaag ggacctaacg tgagaactaa cccgccggtt
    20041 accgacgcac cgagcagagc gagtgagccc agctcacacc cggtcgggtt cagtgcattg
    20101 ttcggcgtta tcgctttttc ccattgcgtt gctgcatgat ggtccgcagc tcctcgatgc
    20161 tcagaggagg ttcctgtgtg tggagcattg cgtggcagtt aggacacacc ggagccaaat
    20221 cgactgttgg acgaagtttg tattcaccgc cacggccagc caaaggtttt ttgtggtgaa
    20281 catgaatgaa gcctcgcccg attgagccat agcgtcgctc aaagctcatc ccgcagacgc
    20341 cacacctcaa accgtgtttc gctatacaag cagcccgtgc acgagggtcg cgctcatagg
    20401 cattaactgt cctttggcaa acggcccctt cgataaactc ttcatcgtcg ccgacctcgt
    20461 cggggaagtt cgaatctggc ggaatcatcc ggcgactaga ctcggtgagc gataggaatt
    20521 tctcttgagg gatctcgtag attgtttgga tgctaaacac aatttccggg aaaaccacgt
    20581 cgcgcggctt atttgcccac tggacgtcga gatggttcgg gaacgcttcg tccggactat
    20641 cgtatggttc cgagtgtttg ttgggatcat agtacccagc gcctttaacc gttcccacag
    20701 ctgcgagttg cttcagcccg cgtctggcga cgagcatgtc gcctcgttgt accgaatggt
    20761 agaatttgtg caacatgccg ctcgagagtg ttgcggcagc tggcttatag tcgcggtatg
    20821 tgcgacgaac aagccgaaga agctcgtctt ccgcgagagc tgatacatcg cccagggcgg
    20881 gccacccgat tgatatcacg ccgtgctcga ggtcgtattt ccagacctgc tcccattcat
    20941 cagggtagcg ggcgtcatat ggggcaatga cccagaaacg tcgcatcgag ccttctcctt
    21001 gtggattgag ccgccgaaca tatactagac gtccgaatga gacgtcttta aatacatctc
    21061 ggcgttctgt ataatccgat tccttcccgg agtcaatggg ttatgacgcc gaccggatcc
    21121 gagacgtctt taaaggcgtc tgccgaagcg aaacccaagc tcctcgatca gttacgccac
    21181 accatccgac tccgtcacta cagcatccgg accgaagaag cctacgtcca gtgggcaaaa
    21241 cgcttcattc tgtttcacaa gaaacggcat ccgcaggaca tgggacggcc ggagattgaa
    21301 gcgttcctca cgcaccttgc cgtccaggga aaggtggcgg cctcgacgca gaaccaggcg
    21361 ctcaacgcca ttgtgtttct cta
//
LOCUS       DBNGTT010000501         5587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_503701, whole genome shotgun sequence.
ACCESSION   DBNGTT010000501 DBNGTT010000000
VERSION     DBNGTT010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_503701"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="AC16BC_11565"
     CDS             <1..542
                     /locus_tag="AC16BC_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242296.1"
                     /translation="PSFVPDAPGTYELGLIVHDGALPSPEDRVFAFITPPLDVDVHPE
                     TVNLKSHGGSKSLTAELASRVVSSFAPLTATDGVTVTATFALTIEYLDASGQPVVFTL
                     SNEDYPGDDRVEAEDRDRCGRVDEYALTLKFARSAFIAGFTDAQGTLRITQPTTVTAT
                     VLGNGVAIGQDTIRVLPPN"
     gene            721..1182
                     /locus_tag="AC16BC_11570"
     CDS             721..1182
                     /locus_tag="AC16BC_11570"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3242297.1"
                     /translation="MAGEIQVGAAAPDFTLKDQDQKEVKLGDFKGNKNVVLAFYPLDW
                     SPVCTKENRCLTDDFPKFSSADTEVFGISIDSVYSHKAWADSLGLKHRLLSDMGGNTA
                     KKYGMYLEDKFITKRATVVIDKGGVVRFVKVQEILTARDDQEILNALKALK"
     gene            1252..1602
                     /locus_tag="AC16BC_11575"
     CDS             1252..1602
                     /locus_tag="AC16BC_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3242298.1"
                     /translation="MDAVHAEPIELPRVNDENFEEYHEAPAAAVFYKIANCHNCDEYE
                     PAIQAAAREFSLKVKFGRGLMHIPGSREIKKRYEFESFPSTHLYKRGKLVFSASERLE
                     FESLANAIREHLIG"
     gene            1715..2956
                     /locus_tag="AC16BC_11580"
     CDS             1715..2956
                     /locus_tag="AC16BC_11580"
                     /EC_number="4.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008473 - ornithine cyclodeaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase, nickel-pincer
                     nucleotide-dependent"
                     /protein_id="HIR3242299.1"
                     /translation="MPTETVELRGHIIDSLLLPKVLDEIMNLGGRFEIVDLAVGHRRE
                     DASRATINVTASDAAQLDLILGRIGQHGALPVRGEDVHLVAADCDGAFPEGFYCTSHF
                     DSWIRREGAWIPVEHPEMDCGIAFDPAAGRAFTIPMHRVRRGDLIVVGHAGVKVAPID
                     RSPERNVFEFMASSVSSEKPKAVVIREMAAHIKAARAAGEKVLVVAGPAVIHTGSGAH
                     LERLIRSGYVQVLFAGNALAAHDIEQALFGTSLGMSLERGIPVEEGHEHHLRAINAIR
                     RVGGIRQAVEQGLLSQGIMHACVTQGVDVVLAGSIRDDGPLPDVITDMVQAQDAMREK
                     IRGVTVAILIATTLHSIATGNILPASVKLICVDINPAVVTKLADRGSFQAMGLVTDVE
                     PFLRTLADSLAQSSPGGLSRG"
     gene            2953..3756
                     /gene="ddaH"
                     /locus_tag="AC16BC_11585"
     CDS             2953..3756
                     /gene="ddaH"
                     /locus_tag="AC16BC_11585"
                     /EC_number="3.5.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylargininase"
                     /protein_id="HIR3242300.1"
                     /translation="MTSRRSLLMCPPDFYGIEYEINPWMDVRRPADAARAQAQWRALY
                     DELTRGLGIPVDLLDPVKGLPDLVFTANAGLVAPGRFIAGRFLHPERQQEQPVYEAWF
                     RRRGYRIDTLPEETCFEGEGDALWLGDTLFAGYRIRSDVRSHQEVGALLGRQVLSLEL
                     VDPRFYHLDTCFCPLDARRALYYPPAFDEYGRRVIAQFVPEPIAVTEEDAARFGCNAV
                     VSGRDVVLQAGCDALETTLRAHGFTPHPVDLSEFHKAGGSAKCLVLWIA"
     gene            3844..4758
                     /locus_tag="AC16BC_11590"
     CDS             3844..4758
                     /locus_tag="AC16BC_11590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR3242301.1"
                     /translation="MLSRRHQWFAAAVAIALVGWAMPGWADDVAWPKVRAKGLLLVDL
                     TQQRVLVEANAGARLAPASLTKVMTALIAFEEGDLNAPATISRHAARATGHRLRLRAG
                     QTFRFGDLINAMLVTSANDACRAVAETVAGTEDDFVDKMNRYAEALGLADTRFQNACG
                     FDAPNHYSTANDLARLATTALANPAFGDAVKIHQGVMHTLDGRRMYRYRTTNRLMSSF
                     DGAVGVKTGFTNRAGRCLIARAIRDEGDLLLVMLNAPRRWWDAAALLTRGFESLAASR
                     RDEDGGPSWRRVEVQADAQTPPGTILPL"
     gene            4936..>5587
                     /locus_tag="AC16BC_11595"
     CDS             4936..>5587
                     /locus_tag="AC16BC_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3242302.1"
                     /translation="MNNVRGLRFAAAFGIVFALPLILTGLARAQDAPAPTVDTGDTAW
                     VLMSAALVMLMTAPGLALFYGGLVRTKNVLGTIMQSVIILCVVTVLWILFGYSLAFGP
                     DVGGFIGGLDWVGLRNVGLDPHPTYGPTIPHQVFMMFQLMFAAITPALITGAFAERMR
                     FSAVLLFTVLWSVLIYFPLAHWVWGGGWLAKLGAVDFAGGAVIHISSGASALACALM"
BASE COUNT          908 a         1756 c         1878 g         1045 t
ORIGIN      
        1 cgccgtcgtt cgtcccggat gcgcctggca cctatgaact tggcctgatc gtgcatgacg
       61 gcgccctgcc gagccccgag gatcgcgtgt tcgcgttcat caccccgccg cttgacgtgg
      121 acgtgcatcc cgagaccgtc aacctcaaga gccacggcgg ctccaagtct ttgactgcgg
      181 agttggcatc ccgcgtggtg tcgtcctttg ccccgttgac cgcgacggac ggcgtgacgg
      241 tcacggccac gttcgccctg accatcgagt acctggacgc ctcgggccag cccgtggtct
      301 ttaccctctc gaacgaagac tatccgggcg atgaccgggt ggaggcggaa gatcgcgacc
      361 ggtgcggccg cgtcgatgag tacgcactga cgctcaagtt cgcccgcagc gcgttcatcg
      421 ccgggttcac cgacgcccag ggcacgctcc gcatcaccca gccgaccacg gtgaccgcga
      481 ccgtgctggg caacggcgtg gccatcggcc aggacacgat ccgggtcctg ccgccgaact
      541 gattcgcata accacggaca ttagggcgtt gtcggtatgg cggggaccgc atcagattgg
      601 cggtcccttg cttgacatcc ccaaaatcgc cgtgatagaa cggggcctct tttcttccgg
      661 tgcgcggtgc gggttcttcc gcacggttgc gcgatttcac atagaacaag gaggggagca
      721 atggctgggg aaattcaagt aggggcggcg gctccggatt tcacgctcaa ggatcaggac
      781 cagaaggaag tgaaactcgg cgacttcaag ggcaataaga acgtagtgct cgcgttctat
      841 ccgttggatt ggagcccggt gtgcacgaag gaaaaccgct gcttgaccga cgacttcccg
      901 aagttttcgt ccgctgatac cgaggtgttc ggcatcagca tcgacagcgt gtactcgcac
      961 aaggcgtggg cggattcgct cggcctgaag caccgcttgc tgtccgacat gggcggcaac
     1021 accgccaaga agtacgggat gtacctggag gacaagttca tcaccaagcg cgccaccgtg
     1081 gtgatcgaca agggcggcgt ggtccgcttc gtgaaggtcc aggaaatcct gaccgcgcgc
     1141 gacgaccagg agattctgaa cgccctcaag gcgctcaagt aaccgtcgac tgacgtgtcg
     1201 tggaggggcg cacgcccgtg cgcccctcca gttatttacc ccgagactcc catggatgcc
     1261 gtacacgctg agccgattga actgccgcgc gtcaacgacg agaacttcga ggagtaccac
     1321 gaggcgcctg cggccgccgt gttctacaag atcgccaact gccacaactg cgacgagtac
     1381 gagcccgcga tccaggccgc ggcccgcgag ttttctctca aggtcaagtt cgggcgaggc
     1441 ttgatgcaca tcccgggcag ccgcgagatc aagaagcgtt acgagttcga gagcttccca
     1501 tcgacgcacc tctataagcg cggcaaattg gtgttctccg cgtcggagcg gttggagttt
     1561 gaatcgttgg cgaatgctat cagggaacat ctgatcgggt aggcgccgtg cgtcgtcggc
     1621 tcgatcgcgc acactgcaaa tccccccaac cccccttttt caaagggggt aagagtagag
     1681 ccttcattct cgagtcactc gacctataac gcgcatgcct acggaaaccg tcgagcttcg
     1741 cggccacatc attgactcgt tgctgctgcc caaggtgctc gacgagatta tgaacctcgg
     1801 cgggcgattt gagatcgtgg atctcgctgt gggccatcgc cgggaggatg cgagccgcgc
     1861 cacgatcaac gtgaccgcgt cggacgccgc gcagctcgac ctgattctgg gacgcatcgg
     1921 gcaacacggc gcgttgcccg tgcggggaga ggacgtccat ctggtcgcgg ccgactgcga
     1981 cggcgcgttt cccgagggat tctactgcac gtcgcacttt gactcgtgga tccgtcggga
     2041 gggcgcgtgg attccggtcg agcacccgga gatggattgc ggcatcgcgt ttgatcccgc
     2101 cgcgggccgc gcgttcacca ttcccatgca ccgggtgcgc cgcggcgacc tgatcgtggt
     2161 cgggcacgcc ggggtcaagg tcgcgccgat cgatcgctcg cccgagcgca acgtgttcga
     2221 gttcatggcg agcagcgtgt cgtcggaaaa acccaaggcg gtcgtgatcc gcgagatggc
     2281 cgcccacatc aaggccgcgc gggccgcggg cgagaaggtg ctcgtggtgg ccgggccggc
     2341 ggtgatccac accgggtcgg gcgcgcacct ggagcgattg atacgatccg ggtacgtaca
     2401 ggtgttgttc gcgggcaacg cgctggcggc gcacgacatc gagcaggcct tgttcggcac
     2461 gtcgctcggc atgtcgctgg agcggggcat tccggtggag gaggggcacg agcaccactt
     2521 gcgcgcgatc aacgcgatcc gccgggtcgg gggcattcgc caagccgtgg agcagggtct
     2581 gctgtcgcag ggcatcatgc acgcgtgcgt gacgcagggc gtggacgtgg tcctggccgg
     2641 gtcgatccgc gacgacgggc cgttgccgga cgtgatcacc gacatggtcc aagctcagga
     2701 cgccatgcgc gagaagatcc ggggcgtgac cgtggcgatt ttgatcgcca ctacgctgca
     2761 ctcgatcgcc accggcaaca tcctgcccgc ctcggtcaaa ttgatctgcg tggacatcaa
     2821 ccccgcggtg gtgaccaaac tcgcggaccg cgggagcttc caggccatgg ggctggtgac
     2881 cgacgtggag ccgtttctgc gaaccctggc cgattcactg gcgcagtcct cgcccggagg
     2941 gttgtcgcgg ggatgacttc tcgtcgatcc ctgttgatgt gtccgccgga cttttacggg
     3001 atcgaatacg agatcaaccc ctggatggac gtgcgcaggc ccgcggacgc ggcgcgcgcg
     3061 caggcgcaat ggcgcgcgct ctacgacgag ctgacccgcg gtctgggcat tccggtcgat
     3121 ctgctcgacc cggtcaaggg gctgcctgat ctggtcttca ccgcgaacgc gggactcgtc
     3181 gcgcccggcc gcttcatcgc cggccgattc ctccatcccg aacgacagca ggagcagccc
     3241 gtctacgagg cgtggtttcg tcgccgcggg taccggatcg acacgttgcc ggaggagacg
     3301 tgctttgaag gggagggcga cgcgttgtgg ctgggcgaca ccctgttcgc ggggtatcgg
     3361 atccgttccg acgtgcgctc gcatcaggag gtcggggcgt tgctcggtcg tcaggttctg
     3421 tcgctcgaac tggtggaccc ccgcttctac catctggaca cgtgtttctg tccgctcgac
     3481 gcgcggcgcg cgctctatta cccgcccgcg ttcgacgaat acggccgccg ggtgatcgcg
     3541 cagttcgtcc cggagcccat tgcggtcacc gaggaagacg ccgcgcggtt cgggtgcaac
     3601 gcggtggtct cgggccgcga cgtggtgctc caggcgggtt gtgatgcgct ggaaacgacc
     3661 ttgcgcgcgc acggcttcac gccccatccc gttgatctgt ccgaatttca caaagccggg
     3721 ggcagcgcca agtgtttggt gttgtggatt gcatagcgcg tgccgagcgt tgacgactcg
     3781 aacggcatcc attatagttg gccccattgt tcccgagggt tcgtcaggcg tgagtccgat
     3841 ttgatgttga gcaggcgtca tcaatggttc gcggcggcgg tggccatcgc attggtcggc
     3901 tgggccatgc ccggttgggc cgacgacgtc gcgtggccca aggtccgcgc caaggggctc
     3961 ttgctcgtgg acctgacgca gcagcgggtg ttggtcgagg ccaacgcggg cgcgcggttg
     4021 gcgccggcga gtctgaccaa ggtcatgacc gcgctgatcg cgttcgaaga aggcgacctc
     4081 aacgcgcccg cgaccatcag ccgacacgcg gcccgcgcca ccgggcatcg cctgcgattg
     4141 cgggccggtc agacgttccg attcggggac ctcatcaacg ccatgctggt gacgtccgcc
     4201 aatgacgcgt gccgggccgt ggccgaaacc gtcgccggca cggaagacga cttcgtcgac
     4261 aagatgaatc gctacgccga ggccctgggg ctggccgata cccgcttcca gaacgcgtgc
     4321 ggcttcgacg cgccgaacca ctattccacg gccaatgatc tggcgcggct cgccaccacc
     4381 gcgctggcga atcccgcgtt cggggatgcg gtcaagattc accagggcgt gatgcacacc
     4441 ttggacggcc gccgcatgta tcggtaccgc accaccaacc gcctgatgtc cagcttcgac
     4501 ggcgcggtgg gggtcaagac gggtttcacc aatcgcgccg gccggtgttt gatcgcgcgg
     4561 gcgatccgcg acgagggcga tctgctgctc gtgatgttga acgccccccg gcgctggtgg
     4621 gacgcggcgg cgttgttgac gcgcgggttc gagtcgctcg ccgcctctcg acgcgacgag
     4681 gacggcggtc cgtcctggcg gcgcgtcgag gtccaggccg acgcgcagac gccgcccggc
     4741 acgatccttc ccctgtagtc tcccctgttc cgaaccggat tctcgatctg atacgaccat
     4801 ggtcggcgca cgccgatcgc cggaccggga taacgcgcct gatttcctgt tgaccccgtg
     4861 ggagcgttat gccaggatac gcgccacgct cgacgttttt gtcgagcgct gtcctcttag
     4921 cctggagtca ctcccatgaa caacgtgaga gggttaaggt tcgccgcggc attcggaatc
     4981 gtgttcgcgc tcccgctgat cttgacgggg ctggcgaggg cgcaggacgc gccggcgccc
     5041 acggtcgata ccggggatac cgcctgggtg ttgatgtccg ccgcattggt catgctcatg
     5101 acggcgccgg gtctggcgtt gttttacggc ggcctggtcc gcaccaagaa cgtcctcggc
     5161 accatcatgc agagcgtcat catcctctgc gtggtcacgg tgttgtggat tctgttcgga
     5221 tacagcctgg cgttcggccc ggatgtgggc gggttcatcg gcgggctgga ctgggtcggg
     5281 ttgcggaacg tgggtttgga tccgcacccc acgtacgggc ccacgattcc ccaccaggtt
     5341 ttcatgatgt ttcagttgat gttcgcggcg atcacgccgg cgctgatcac cggcgcgttc
     5401 gcggaacgga tgcggttctc cgcggtgttg ctcttcaccg tgttgtggtc ggtgttgatc
     5461 tatttcccgc tcgcgcattg ggtgtggggc ggcggatggc tcgcgaagct gggggccgtg
     5521 gacttcgcgg gcggtgccgt cattcacatc agctcgggcg ccagcgccct cgcgtgcgcg
     5581 ctgatgc
//
LOCUS       DBNGTT010000502         1859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3441662, whole genome shotgun sequence.
ACCESSION   DBNGTT010000502 DBNGTT010000000
VERSION     DBNGTT010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3441662"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="AC16BC_11600"
     CDS             complement(<1..262)
                     /locus_tag="AC16BC_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242303.1"
                     /translation="MGGIVTTGPHTEYPGITQTEDAAGNARYLGEIVNNSNRVACNIG
                     VSINSYDASGVLLTNPGNQQFGFANLLGETFRFSSSTTQTMEN"
     gene            complement(364..1284)
                     /locus_tag="AC16BC_11605"
     CDS             complement(364..1284)
                     /locus_tag="AC16BC_11605"
                     /inference="COORDINATES: protein motif:HMM:NF021445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HIR3242304.1"
                     /translation="MPGVSLQPLAPGDRSWIEPLLDGAPSELGTRTFTGVYIWREHFD
                     YAWTWIDGYFCLFARYGPHTYMPWPPLPGPAADPGRFAAVVGTAFEVMAASPDQGSAA
                     RIEAVDQWERPSFEAAGYEVVPHATEYVYQRQAFVELRGNAYKSKRWAWNDFVKQQVF
                     TLDPLADRHVPECLALYRRWRDLKARRTADDLARAMAEDAESAHRIAVTERDALGLRG
                     LVVTIEGRVAGYTLGGAVRPDLFGVWLEIADPERRGLATYLFREFCRAQTGVEWITAL
                     DDSGLASLANAKRSYRPARLVHSFLVRRRG"
     gene            complement(1289..>1859)
                     /locus_tag="AC16BC_11610"
     CDS             complement(1289..>1859)
                     /locus_tag="AC16BC_11610"
                     /inference="COORDINATES: protein motif:HMM:NF015639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR3242305.1"
                     /translation="RIFMNRNRSGSTFSLPGDDLPQIVPGDLCLRCDVCCRFPERESF
                     LRPYFTAQERERAIAAGLAPDRLPDPAGGRIELIRHPTGDGYICPAFDPATQYCGIYD
                     ARPLDCRIYPLALMRDEDDQGALLGLDTKCPYVRDAAQEARLLAYARRVGDLFQEPAL
                     RNVLSSNPELVNRFQDDVRPICDVGSDDE"
BASE COUNT          356 a          629 c          572 g          302 t
ORIGIN      
        1 agttctccat ggtctgcgtg gtcgacgaag aaaaccggaa cgtttcgccc agcaaattgg
       61 cgaatccgaa ctgctggttc cctgggttgg tcagcaaaac tcctgacgca tcataggagt
      121 tgatcgacac cccgatgttg cacgcaactc tattgctgtt gttgacgatt tcaccaaggt
      181 accgcgcgtt cccagccgca tcctcagtct gggtgatccc gggatactcc gtgtggggcc
      241 ccgtggtgac gatccccccc acgaattcga gcggaggtgg cggcggcccc ggcaacgggt
      301 cgcctggccc aggcgactca aggccgcagc cggatgttgc caggacgacc agcagcgtcg
      361 cgatcagccg cgacgccgga ccaggaacga atgaacgagc cgggccggac ggtacgagcg
      421 cttcgcgttc gccaatgacg ccaaccccga atcgtccaac gccgtgatcc attcgactcc
      481 agtctgcgcg cggcaaaatt cccgaaagag gtacgtggcc aacccgcgtc tttcgggatc
      541 ggcgatttcc aaccacaccc cgaacaggtc gggccgcacc gccccgccca gggtgtaccc
      601 cgcgacccgg ccctcgatcg ttacgaccag gccccgcaac cccaacgcat cgcgctcggt
      661 caccgcgatc cggtgcgcgg actcggcgtc ctcggccatg gcgcgggcca gatcatccgc
      721 ggtccgacgg gccttgagat cccgccatcg acggtacaag gccagacatt cgggcacgtg
      781 acggtctgcc aaagggtcca gagtaaagac ctgctgcttc acgaaatcat tccaagccca
      841 acgctttgat ttgtaagcgt tccccctgag ctcgacaaat gcttggcgct gatacacgta
      901 ctcggtggcg tggggaacga cctcgtaacc cgcggcctcg aagctcgggc gctcccactg
      961 gtccacggcc tcgatgcgcg ccgcactccc ctggtccgga gacgcggcca tgacctcgaa
     1021 ggctgtgcca accacggccg cgaatcgtcc tggatcagcc gcgggccctg gaagcggagg
     1081 ccagggcatg tacgtgtggg ggccgtagcg ggcgaacagg cagaaatatc cgtcgatcca
     1141 agtccacgcg tagtcgaagt gttcgcgcca aatgtacacg ccggtgaacg tgcgcgtccc
     1201 caactcgctc ggcgccccat ccagcagcgg ttcgatccac gaccggtccc ccggcgcgag
     1261 cggctggagc gacacgccgg gcaccggtct actcgtcgtc gctcccgaca tcgcagatgg
     1321 gtcgcacgtc gtcctgaaat cggttgacca gctcggggtt gctcgacaac acattgcgca
     1381 gcgcgggctc ctgaaacagg tcgcccaccc gccgggcata cgcgagcagc cgcgcctctt
     1441 gcgccgcgtc ccgcacgtag gggcacttgg tgtcgagccc gagcagcgcg ccctggtcgt
     1501 cttcatcacg catcagcgcc aatgggtaaa tccggcagtc caatggtcgc gcgtcgtaga
     1561 tgccgcagta ttgcgtggct gggtcgaacg cgggacagat gtatccgtcc ccggtaggat
     1621 gccggataag ttcgatccgc ccgcccgcgg gatcaggcaa gcgatccggc gctaacccgg
     1681 ccgcaatggc gcgctcgcgt tcctgcgccg tgaagtacgg ccgcaaaaaa ctctcgcgct
     1741 cgggaaagcg gcaacacacg tcgcaccgca ggcatagatc gccgggcacg atctggggta
     1801 gatcgtcgcc gggcaaagaa aaggtggatc ccgagcgatt gcgattcata aatatccgg
//
LOCUS       DBNGTT010000503         3385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2548076, whole genome shotgun sequence.
ACCESSION   DBNGTT010000503 DBNGTT010000000
VERSION     DBNGTT010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2548076"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /locus_tag="AC16BC_11615"
     CDS             <1..1842
                     /locus_tag="AC16BC_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR3242306.1"
                     /translation="RTDGTDDRALAIRHSGFQSSWSPDGTRLAFSQDETWNNYATYSD
                     LYSVDTRDGHVRRHTRGARAGDPDFSPDGAQLVFVGRELGKSRLARLDLASGAIHALT
                     DWRDDVQYATPRWSPDSSHIAAVVWSKGRQDIALLDVATRELAFVTHDRALDLTPNWS
                     RDGRTLYFTSDRTGVYNVFAYSLADGSLTQATNVVGGAFAPAPTADGRLAFSNYTARG
                     FDLHLTTPMAIPVSAEAAEPDRTPSPPVPPIDLPAPKPYSPLPTLAPRFWAPVLSTDE
                     EGGQYGVQTGGLDVLGYHKYALTGVYGSRSHRVSYGFTYRYDRWHPSFDLAVSDVAAL
                     YDDFFVSPNGDTGEYWERRQRLAADITLPIFKTRWSQALSIGYRGERLSPLSVTPAGA
                     QSPAEGTLAGVRAAWRYDNAHEFGYSISPEGGRRLSARFQRNMDSFGSDFETTRYVGA
                     WYEYLSLPRLRHHVLALRAVGGTSSGDVLPQRTFQLGGATMSEELLEDDADTVLLRGY
                     RARAIRGQRMLVGSVEYRFPIWNVERGVTTYPIFLHRLHGALFADHGNAWDPSTPAPD
                     YKTGVGAELGTDMTLGYRLRIRLRVGVAVGLDDQGETQGYLSAGHAF"
     gene            1912..2304
                     /locus_tag="AC16BC_11620"
     CDS             1912..2304
                     /locus_tag="AC16BC_11620"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3242307.1"
                     /translation="MIVLLIIAVAGCAGTPDQDAVTIPASRIEELTKALATRVDRPPI
                     PKGAVAHPYYPDYAMRRPKPGMVLLKIDVDAKGRVAAVQVVESTNVFLESAARQAALQ
                     WSFEPAMRHGLPVAATIVVPVEFTQGAR"
     gene            2641..3210
                     /locus_tag="AC16BC_11625"
     CDS             2641..3210
                     /locus_tag="AC16BC_11625"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR3242308.1"
                     /translation="MRSVIEYIKSDLYRYAGTTSYRVLFQHLRKNPSFQYSFWFRLCK
                     SRYRILRAVAKFKHRRLSDRYGIQIPPTTEIGYGLYLGHHMCIVINQSAKIGNNCNLS
                     PFTTIGANHGKAADIGNNVYIGPNVSIVENVSIHHNSTIGAGATVVKDVPENATVAGC
                     PAKVISMKEPGRYIKNRWADNQPDVSTTQ"
BASE COUNT          634 a         1151 c         1029 g          571 t
ORIGIN      
        1 cggaccgacg gcacggacga ccgcgcgctg gcgatccgcc attccgggtt ccaatccagt
       61 tggtcacccg acgggacccg gctcgcgttt tcgcaggacg agacctggaa caattacgcg
      121 acctacagcg atctatacag cgtggacacg cgcgacggac acgtccggcg gcacacacgc
      181 ggcgcgcggg ccggcgatcc cgacttctcc cccgacggcg ctcaactggt gttcgtgggc
      241 cgcgaactcg gcaagagccg gctggcccgg ctcgatctcg cgagcggcgc gattcacgcg
      301 ctcaccgact ggcgcgatga cgtccagtac gccaccccgc gctggtcccc ggacagttcg
      361 cacattgcgg ccgtggtctg gtccaagggg cgccaggaca tcgccctgct cgacgtggcc
      421 acgcgcgagc tcgcgttcgt cacgcacgac cgcgccctgg acctcacccc caattggtct
      481 cgcgacggcc gcacgctgta cttcacgtcg gaccgaaccg gcgtctacaa cgtgttcgcc
      541 tactcgctcg ccgacggctc gctgacgcaa gcgaccaacg tggtcggggg cgcgttcgct
      601 ccggcgccca ccgccgacgg ccgcctggcc ttttccaact acaccgcgcg gggcttcgac
      661 ctgcacctga ccacgccgat ggcgattccg gtatccgccg aagccgccga gccggaccgc
      721 acgccgtccc cgcccgttcc gccgatcgat ctacccgcgc ccaagcccta ctcgccgctg
      781 cccacgctgg ccccgaggtt ttgggccccg gtcttgagca cggacgaaga gggcgggcag
      841 tacggcgtcc agaccggggg gctcgacgtg ctgggctacc acaagtacgc gttgaccggc
      901 gtgtacggat cccgcagcca tcgcgtgagt tacggattca cctatcgcta cgatcggtgg
      961 cacccctcgt tcgacctggc cgtgtccgac gtggccgcct tgtacgacga cttcttcgtg
     1021 tcgcccaacg gcgacaccgg cgaatactgg gaacgccggc aacgcctcgc cgccgacatc
     1081 accctcccga tcttcaagac ccggtggtcg caggcgttgt cgatcggata ccgcggagag
     1141 cgtctgtctc ccctgtcggt cacgcccgcc ggcgcccagt ccccggccga gggcacgctc
     1201 gccggcgttc gcgcggcctg gcgctacgac aacgcgcacg agttcgggta ctcgatcagc
     1261 ccggaagggg gccgacgcct gtcggcgagg ttccagcgca acatggactc cttcggcagc
     1321 gacttcgaga cgacgcgata cgtgggggcc tggtacgaat acctgtcgct gccgcgactc
     1381 cggcaccacg tcctcgccct ccgcgcggtg ggcgggacct cgtccggcga cgtgctgccg
     1441 cagcgcacct ttcagctcgg cggggcgacc atgtccgaag aattgctgga agacgacgcg
     1501 gatacggtcc tgctccgcgg ctaccgcgcg cgggcgatcc gcggccagcg catgctggtg
     1561 ggaagcgtgg aataccgatt cccgatctgg aacgtggaac gcggcgtcac cacctatccg
     1621 attttcctcc accgcctgca cggcgcgttg ttcgcggacc acggcaacgc ctgggacccg
     1681 tccaccccgg cgccggacta caagaccggc gtgggcgcgg aactgggcac cgacatgacc
     1741 ctcgggtacc ggctgcggat caggctgcgg gtcggcgtgg ccgtcggctt ggacgaccag
     1801 ggcgagaccc agggctacct gagcgcgggg cacgcgtttt agcccggagc atcgcctgtg
     1861 cggcggacac gacgttccga cacgtcatcc cggaccgccc ccggcctcgc gatgatcgtg
     1921 ctcctgatca tcgcggtcgc ggggtgcgcg ggaacgccgg accaggacgc cgtgacgatt
     1981 ccggcctcgc gcatcgaaga gctcaccaag gccctggcca cgcgcgtgga tcgaccgccg
     2041 atcccgaaag gcgcggtggc gcacccctac tatcccgact acgccatgcg tcgccccaaa
     2101 cccggaatgg tgctgctcaa gatcgacgtg gacgccaaag gccgggtcgc ggccgtgcaa
     2161 gtcgtcgagt ccacgaacgt gttcctggaa tccgcggcgc ggcaggccgc gctgcagtgg
     2221 tcgttcgagc ccgccatgcg gcacgggctc ccggttgcgg ccaccatcgt ggtgccggtc
     2281 gagttcacgc agggcgcacg gtgactccgg accccgcgct gacgcacggc accgcataac
     2341 ggcagagact ccagggggtg cgagctgagc ggtagaggcc ccggcatcca gcaacgtgac
     2401 cgcgccacgt gcttgaaccg tgctccgccg tatgctaagg tcactgcaaa ttttcgatca
     2461 ttaacggtgt gcgggaccga cccagcaggc agacaggaag cggacatgac gggttaagtc
     2521 cttccgccat tggcaccatc cctccctgtt tggatcagga ccgttgagaa agcgtttacc
     2581 acagtcaaac catcgtatca acgaccgtat ggctccgagg attggcatta agcgaagaca
     2641 ttgcgcagcg tgattgaata tatcaagagc gatttatatc ggtacgccgg tacgacttcc
     2701 tatcgagtct tgtttcagca cctgcgcaag aatccatcct ttcaatacag cttctggttc
     2761 aggctctgta aatcgcgtta tcggattctg cgggccgtcg caaaattcaa gcatagacga
     2821 ctctcggaca ggtacggcat tcaaattcca cccacgacag agataggata tggactctac
     2881 cttggacatc acatgtgtat tgtgattaat caatctgcca aaatcggaaa caactgcaac
     2941 ttaagtcctt ttacaaccat tggggcgaac catgggaaag cggctgacat tggcaataat
     3001 gtgtatatag gccccaatgt ctctatcgtc gaaaatgtaa gtattcacca caacagcacc
     3061 attggggctg gggccactgt tgtcaaggat gtccccgaaa atgcgacggt tgcaggctgc
     3121 cctgcaaagg tcatttcaat gaaggagccc gggagatata tcaagaatcg ttgggcagat
     3181 aatcagccgg acgtctcgac aacacaatag ccgtagcaag gcgtgccatc gctcgcccgt
     3241 aacgacgcga attccagacg ccttccattc ggaggcacca ctatcgtcct cgcacaatcc
     3301 acttcctccc ctcctccaac gccagcatgc cgagggcaaa ggggaccacg aataaccaga
     3361 cagtgcccgg gatcggggcg gtgcc
//
LOCUS       DBNGTT010000504         9700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1143763, whole genome shotgun sequence.
ACCESSION   DBNGTT010000504 DBNGTT010000000
VERSION     DBNGTT010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1143763"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="AC16BC_11630"
     CDS             complement(<1..120)
                     /locus_tag="AC16BC_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HIR3242309.1"
                     /translation="MRIGTPLSPSATRVMLLGAGELGKEVIIALQRFGVEVIAV"
     gene            119..1144
                     /gene="hpnC"
                     /locus_tag="AC16BC_11635"
     CDS             119..1144
                     /gene="hpnC"
                     /locus_tag="AC16BC_11635"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - geranylgeranyl diphosphate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HIR3242310.1"
                     /translation="MDQPPGEGGIIARRGGSAEAARCTLTLLESLCYHQRMGSLPSAP
                     RDFGLQEEKVWSLEESYAHCEQVARAHYENFPVGSWLLPADKRKYLWAIYAFARMADD
                     FADEAQYLDNLRLPLLENWEGQLLQCMWRKPQHPVFIALKDTIETFRLPVEPFQDLLM
                     AFKMDVFVERYKTYDDLLEYCRLSANPVGQLVLSVFGYRDPALHELSDSICTALQLTN
                     HWQDIAIDAKKPRIYVPIEDMERHGYDEARLHSNVADEAFRGMMADLVGRTRALFDHG
                     QPLLDRVGQDLAFELRLVWLGGTAILDRIESRGYDVFRARPTLGVGGKALLLAKAALG
                     SGRRASP"
     gene            1141..1974
                     /gene="hpnD"
                     /locus_tag="AC16BC_11640"
     CDS             1141..1974
                     /gene="hpnD"
                     /locus_tag="AC16BC_11640"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046905 - 15-cis-phytoene synthase
                     activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HIR3242311.1"
                     /translation="MSAVVLERPASSGSNFRYAFLVLSRPQRRALDAVYGFCRAADDA
                     VDHAASQDTARAALLTWRRELDRIYAGNPDTPVGQALAPVVKTLHLPREHFDELLLGV
                     AMDIGRPRYQTIQELSRYTYRVASTVGLLCLPIFGVEGEQARAYAIERGLSLQLTYII
                     RDVKADAAMGRIYLPIEELKAFGCGEEDVLAGRHTPAFERLMAFECRRAREAHARARS
                     LVPDRRKLLAPEIMGRTYEALLDRIEASGYRVMDEPIRLTAPTKAAIALKSWVGAHLG
                     G"
     gene            1976..2314
                     /locus_tag="AC16BC_11645"
     CDS             1976..2314
                     /locus_tag="AC16BC_11645"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3242312.1"
                     /translation="MGLADNACVPCRGGVPPLPRERVEKLLLELEPGWAATHDDTRIE
                     RTYRFTEFADAMAFANRVGEIAEREGHHPDLHVGWGRCRVEIWTHKINGLTESDFFLA
                     AKTDRAFKDT"
     gene            complement(2484..3707)
                     /gene="argJ"
                     /locus_tag="AC16BC_11650"
     CDS             complement(2484..3707)
                     /gene="argJ"
                     /locus_tag="AC16BC_11650"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR3242313.1"
                     /translation="MTRQADAPIHVPGGVTAPKGFRAAAVSAGIKKQDRPDLALLVSE
                     VEASVAGVMTANRIKAAPLLIDQPRIKRGKGRAIVINSGCANACTGPEGLEDAKVMAS
                     ETARVLSLDERDVLVASTGVIGTRLPMDRVRGGIMRAAAQLTPSGGSQAATAIMTTDT
                     MPKEIAIRETSGRVAITVGGMAKGSGMIHPQMGTMLAFLTTDAAVPPRTLQAVLKRCV
                     DATFNTIDVDGETSPNDLVVCMANGLAKNTPVTSGAGLTTFEAMVRQVCERLALMIVR
                     DGEGASKLVTLRVKGAANAASARRVLLSVARSPLVKTAWNGEDANWGRTISAIGNAGV
                     PIRPERIALYIGPVAVVRQGVGMGTSAEAQAAVILKQSEFSVTVDLGQGKAEAVLRTC
                     DLSQDYVRINASYRS"
     gene            complement(3704..4762)
                     /gene="argC"
                     /locus_tag="AC16BC_11655"
     CDS             complement(3704..4762)
                     /gene="argC"
                     /locus_tag="AC16BC_11655"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3242314.1"
                     /translation="MAPSKPIRVAVVGATGYTGGELLRLLAGHPRVTVAAVTSEQSAG
                     KSVADVFPSLARIIQLPLAAFNAEKIAADVDFAFVALPSGAAMDAAASLAATGCRVVD
                     LSPDYRFPDPAVYQRWYGGPHRHQKLLAKAVYGLPELHRRKIAKARVVGNPGCYPTGA
                     LIALAPLASKGLLTPGAPIIIDAKSGISGAGRGASLPYHFPEANEGMMAYKVGRHRHQ
                     PEIVQEVARLARGPAAVLFTPHLAPMTRGIFCTIYVETTKPLSSERVAAFYRTAYRNE
                     PFVRVLEPGESPQTKAVWGSNYCDIGGVADPETNRVVLMAAIDNLVKGAAGQAIQNMN
                     LMCGFPETLGLSAPPVFP"
     gene            complement(4910..5299)
                     /gene="rpsI"
                     /locus_tag="AC16BC_11660"
     CDS             complement(4910..5299)
                     /gene="rpsI"
                     /locus_tag="AC16BC_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3242315.1"
                     /translation="MATQYGGTGRRKNAVARVILRPGNGTILINERPYEEYFPRDAWK
                     LQVRQPLESTAVMGRYDVSVNVAGGGLSGQAGAVRHGIARALLEVNTALRPKLKADGL
                     LSRDPRVKERKKYGQKGARRRFQFSKR"
     gene            complement(5299..5757)
                     /gene="rplM"
                     /locus_tag="AC16BC_11665"
     CDS             complement(5299..5757)
                     /gene="rplM"
                     /locus_tag="AC16BC_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3242316.1"
                     /translation="MNRTYSAKKADVISAWHVIDAEGQILGRLAVEAAVLLRGKHKPI
                     FTPHVDTGDHVVVINAEKVKLTGDKLRQKMYRRHSGYPGGLKEVSAGTLIREKPERLV
                     TLAIKGMLPKNKLGKHMATKLRVYRGAAHPHAAQTPQPFTIKPRRQGGAS"
     gene            5952..6467
                     /locus_tag="AC16BC_11670"
     CDS             5952..6467
                     /locus_tag="AC16BC_11670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3242317.1"
                     /translation="MLRLLAIALVGLLLAACSGTPLPREAKGRPGLDLDALRADSARP
                     ISETRQRVIATAAQLVGTPYKFGGTTPKGFDCTGYLMYVFRKAANVALPRTSMEQIQA
                     GDPIPPSQLQPADLVYFRIDRKKDLHLGLYLGEGRFIHAPSSGGAVNIQRLDMEYWRT
                     RFLGARRVLSL"
     gene            complement(6667..8718)
                     /gene="rhaD"
                     /locus_tag="AC16BC_11675"
     CDS             complement(6667..8718)
                     /gene="rhaD"
                     /locus_tag="AC16BC_11675"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HIR3242318.1"
                     /translation="MKSLWSDRDASGLDGLDALVYTSRLIGASPNLVLWGGGNSSVKV
                     MGKDHVGRPTRTLYIKGSGSDMKTITAQQFTPLRLEDLLPLIHRAAMTDEEMVAYQAK
                     STLEPGAPKPSIETLLHAFIPAAHIYHTHADAICTLTDIANSRDVIEELYRDDIVVVP
                     YSRPGFLLSKRTGEAVAAHPRARAVILDKHGLITWGATAKAAYLDTVKYVSMAERYIA
                     SKQPRRARRAPAIPRAAAAHRRETAAAIAPVLRGEMSRGKPVVLLYDDRPEVLAFTRS
                     MDPKTLCGPFTPDHLLYTKPWPLALDPKAARDERTLLVEAPKALERYRTRYMEYFTKY
                     KTPGVTQGDPNPRIVLIPGIGMFTTGKNRRETLIARDLYLHTMGVIQSTHALGGYRPL
                     PPRDLCDFEYWPMENYKLTLAPPEKSLSRRIALITGAAGGIGRGIAERLLAEGAMVAM
                     TDVDLDAARKLTEEYCRRFGDGHAVAIEMDVTNERSVRRAFADTALCFGGLDVFVSNA
                     GIAHCAPVDRLSKADWERSVAVNATGHLLASQEAIRIMRAQGLGGSIVVISTKNVLSP
                     GQDFGAYSASKAAQNQLAKIMAIENARHGIRVNMVNPDAVFLNSGLWSPEIRAERAKA
                     HGISVEQVEDFYAKRNLLGAKILPHDVAEAVLFFASNRSAKTTGATLPVDGGVKDAFP
                     R"
     gene            complement(8782..9546)
                     /locus_tag="AC16BC_11680"
     CDS             complement(8782..9546)
                     /locus_tag="AC16BC_11680"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR3242319.1"
                     /translation="MPRTRAGTYWHGMAQLGLKLDHVATLRQVRGGIEPNVAAAALLA
                     EMGGADWLIAHLREDRRHIQDRDLYGFQHVAPSRLTLEISAADRMLKFALELRPARVT
                     VVPEHPMELTTEGGLDMIGKREIVANAAASLAQAGIPVTLLIQPSVDDVKACHRLGQP
                     GLSVELNATAYRAAKTPLERRAWEERIVTAAKLAAKVGMPVQLGHGLTLDLVRAFAPV
                     PEIAQFNVGHAVIARAALIGIEAAVREMKTAATARA"
BASE COUNT         1594 a         3197 c         3175 g         1734 t
ORIGIN      
        1 gaccgcgatg acctccacgc cgaaccgttg caacgcgatg atgacttcct tgcccaactc
       61 gcccgcgccc aacaacatga cgcgcgtggc cgaagggctc aagggggtgc cgatgcgcat
      121 ggaccaacct ccgggagagg gtgggatcat agcacggcgc ggggggagcg cggaagccgc
      181 gcggtgcacg ttgaccctgc tggaaagcct gtgttatcac cagcgcatgg gcagcctccc
      241 gtcagcgccc cgcgacttcg ggctccagga ggaaaaggtc tggagcctgg aggagtccta
      301 cgcgcattgc gagcaggtcg cgcgcgcgca ctacgagaac tttcccgtgg gctcgtggct
      361 cttgcccgcg gataaacgca aatacctgtg ggccatctac gcgtttgcgc gcatggcgga
      421 tgactttgcc gatgaagcgc agtacctgga caatctgcgc ctgcccttgc tggaaaactg
      481 ggaaggccag ctcctgcagt gcatgtggcg caagccccag cacccggtgt tcatcgcgct
      541 caaggacacg atcgagacgt tccgcttgcc ggtcgagccg ttccaggatc tactcatggc
      601 gttcaagatg gacgtgttcg tggagcggta caagacgtac gacgatttgc tcgagtactg
      661 ccgcttatcc gcgaatcccg tgggccaact cgtcttgtcg gtctttggat accgcgaccc
      721 cgcattgcac gaactgtccg actccatctg caccgcgctg caactgacca atcactggca
      781 ggacattgcc attgacgcga agaagccccg gatttacgtg cccatcgagg acatggagcg
      841 gcacggatac gacgaggcgc gcctgcactc caacgtggcg gacgaggcgt ttcgtgggat
      901 gatggcggat ctggttggcc gcacccgcgc gttgttcgac cacggccaac cgttgctcga
      961 tcgcgtggga caagacctcg cgtttgaact gcgcctggtc tggctcggcg gcaccgcgat
     1021 tctggaccgc atcgagtcgc gcgggtacga cgtgtttcgc gcacggccca cgttgggagt
     1081 cggcggcaaa gccctgttgc tggccaaggc cgcgctcggt tccggacgcc gagcctcgcc
     1141 gtgagcgccg tggtcctgga gcgccccgcg tccagcggca gcaacttccg ctacgcgttc
     1201 ctggtgctct cgcggccgca gcgccgcgcc ttggacgcgg tgtacgggtt ttgccgcgcg
     1261 gcggacgacg cggtggatca cgcggcgtcc caggataccg cgcgcgccgc gctgctgact
     1321 tggcggcgtg aattggatcg catctacgcc gggaacccgg acacgccggt gggccaagcg
     1381 ctcgcgccgg tggtcaagac gctccaccta ccccgcgagc acttcgatga gttgttgctc
     1441 ggcgtggcca tggacatcgg ccgccctcgc tatcagacga tccaggagct gtcgcggtat
     1501 acctatcggg tggcgtccac cgtgggactc ttatgcctgc ccatcttcgg ggtcgagggc
     1561 gagcaggcgc gcgcctacgc gatcgagcgc gggctgtcgc tgcaactgac ctacatcatc
     1621 cgcgacgtga aagccgacgc ggccatgggg cggatctacc tgccgatcga ggagctcaaa
     1681 gcctttggtt gcggggagga ggacgtgttg gcgggccgtc acaccccggc gttcgagcgc
     1741 ctgatggcgt ttgaatgccg ccgggctcgc gaagcccacg cgcgcgcccg cagcctcgtc
     1801 cccgaccgac gaaaactcct ggcgcccgag atcatgggcc gcacgtatga ggcgctgttg
     1861 gatcggatcg aggcgtccgg gtaccgggtg atggacgagc cgatccgcct caccgcgccc
     1921 accaaggccg ccattgccct caagagctgg gtgggcgcgc acttaggagg ataatatggg
     1981 gcttgccgac aatgcctgcg tgccctgccg gggcggggtc ccgccgctgc cgcgcgagcg
     2041 cgtggagaaa ttgctcttgg aactcgaacc gggctgggcc gcgacgcacg acgacacccg
     2101 gatcgaacga acctaccgct tcacggaatt cgccgacgcc atggcctttg ccaaccgcgt
     2161 gggcgagatc gcggagcgcg aaggccacca cccggatttg cacgtgggct ggggccgctg
     2221 ccgcgtggaa atttggacgc acaagatcaa cggcctgacg gaaagcgatt tttttctggc
     2281 cgcgaaaacc gaccgcgcgt tcaaggacac ctaatagttc atgatttttc ggttccgttg
     2341 acggcgaatt tcccgtcgac ggctgagcga caagaagggc ggcggatgca aggcggagcg
     2401 aggcgccgcg ccggagcgta ctcgtttgta cgtgaggacg cggcgccgaa gcgacaccgc
     2461 cgcagacgcc cccttatcgt cgctcaggac ctgtaggagg cgttgatgcg gacatagtct
     2521 tgcgacagat cgcaggttcg caacaccgct tcggcctttc cctgacccag atccacggtc
     2581 acgctgaatt ccgactgttt gaggatcacc gcggcctggg cttccgccga ggtgcccatg
     2641 cccaccccct gccggacgac cgccacggga ccgatataca gcgcgatgcg ctcgggccgg
     2701 atcggcacgc ccgcgttgcc gatcgcggaa atggttcggc cccagttggc atcctcgccg
     2761 ttccacgcgg tcttgaccag gggcgagcgc gccaccgaga gcaacacgcg ccgggccgac
     2821 gcggcgttgg ccgcgccctt gacccgcagc gtcaccagtt tggacgcgcc ctcgccgtcg
     2881 cgcacgatca tcaacgccag gcgttcgcac acctgccgga ccatggcctc aaacgtggtc
     2941 aaccccgccc ccgaggtaac cggcgtattt ttcgccaagc cgttggccat gcagaccacc
     3001 aggtcgttgg gactggtctc gccgtccacg tcgatggtgt tgaacgtcgc gtccacgcag
     3061 cgcttgagaa ccgcctgaag cgtgcgcggc ggcaccgcgg cgtcggtggt caggaacgcg
     3121 agcatggtgc ccatctgagg atggatcatc cccgatccct tggccatgcc gccgaccgtg
     3181 atcgcgaccc ggccgctggt ttcgcggatg gcgatttcct tgggcatggt gtccgtggtc
     3241 atgatcgcgg tcgcggcctg cgatccgccc gagggcgtga gttgggccgc ggcccgcatg
     3301 atcccgccgc ggacccggtc catgggcagg cgcgtgccga tcacgccggt cgacgcgacc
     3361 agcacgtcgc gttcatccag cgacaacacg cgcgcggtct cggacgccat gaccttcgcg
     3421 tcttccaacc cctcgggccc ggtgcacgcg ttcgcgcagc cgctgttgat gacgatcgcg
     3481 cggcctttcc ctcgcttgat ccgcggctga tcgatcagca gtggcgcggc cttgatccga
     3541 ttggcggtca tcacgccggc caccgaggct tcgacctcgg acaccagcag cgccaggtcg
     3601 ggccggtcct gttttttgat gcccgcggac acggccgcgg cgcgaaaacc cttgggcgcg
     3661 gtgacgccgc cgggcacgtg aatgggtgcg tcggcctgcc gcgtcatggg aacaccggag
     3721 gcgcggacag cccgagcgtt tccggaaacc cacacatgag gttcatgttc tgaatcgcct
     3781 gccccgccgc gcctttcacg agattgtcga tcgccgccat cagcaccacc cgattggttt
     3841 cagggtcagc cacccccccg atgtcgcagt aattcgaccc ccacaccgcc ttggtctgcg
     3901 gagattctcc cggttcaagg acccgcacaa agggttcgtt gcggtacgcg gtccgataga
     3961 acgccgccac ccgctcggaa ctgagcggct tggtcgtttc gacgtagatg gtgcagaaaa
     4021 tgccgcgcgt catcggcgcc agatgcggcg tgaacaacac ggcggccgga ccacgggcca
     4081 gccgcgcgac ctcctggacg atctcgggct ggtgccggtg ccggccgacc ttgtaggcca
     4141 tcatgccctc gttcgcttcc ggaaagtgat aggggagcga cgcgccgcga cccgcgcccg
     4201 agatcccgga tttggcgtcg atgatgatgg gcgcgccggg cgtgagcagc cccttgctcg
     4261 ccagcggcgc gagagcgatg agcgcgcccg tggggtaaca cccgggattg cccaccaccc
     4321 gcgccttggc gatcttgcgg cggtggagct cgggaagtcc gtacacggct ttggccagca
     4381 gtttctggtg ccggtgcggc ccgccgtacc agcgctggta caccgcggga tccgggaagc
     4441 gatagtccgg actcaaatcc accacgcggc acccggtcgc ggcgagggaa gccgcggcgt
     4501 ccatggccgc gccggacggc agcgccacga acgcgaagtc gacgtccgcc gcgatttttt
     4561 ccgcgttgaa cgcggccagg ggaagttgga tgatccgcgc gagcgaagga aacacgtcgg
     4621 ccacggattt gccggcggat tgttccgagg tcacggcggc cacggtgacg cgggggtgcc
     4681 cggccagcag gcgcagcaac tcgccccccg tgtacccggt cgcgccgacc acggcgaccc
     4741 gaatcggttt ggatggtgcc atcggtcctc tcagtgagac gcccgcgtca gcgcgcgccg
     4801 aggcgccggc gcaaacccaa cgccgaggtg cgcgacgcca tgcgtcaatc ccataaaaaa
     4861 ggggaaggtc gatcgacctt ccccctcgaa tccagtcagg ttaaggtcgt taccgcttcg
     4921 aaaactggaa ccgccgacgg gcgcccttct gcccgtattt cttgcgctct tttacgcgcg
     4981 gatcgcggct cagcaagcca tcggctttga gcttgggacg caatgcggtg ttgacttcca
     5041 gcaaggcccg cgcgattccg tgacgcaccg cgcccgcctg cccgctcaac ccgccgccgg
     5101 ccacgttgac cgacacgtcg taacgcccca tcaccgccgt agattcgagc ggctgccgca
     5161 cttgcagctt ccacgcgtcg cgcggaaagt actcttcgta ggggcgctcg ttgatgagga
     5221 tggtgccgtt gcccggccgc aggatcaccc gcgccaccgc gttcttgcgc cgccccgttc
     5281 cgccgtactg tgtggccatt atgatgcgcc tccttgccgg cgcggcttga tcgtgaaggg
     5341 ttgcggcgtc tgagccgcgt gaggatgcgc cgcgccgcgg tacacccgga gtttcgtggc
     5401 catgtgtttg cccaacttgt tcttcggcag catgcccttg atcgccagcg ttaccaggcg
     5461 ctcgggtttt tcccggatca acgtgcccgc ggaaacttcc ttgaggccgc ccggataccc
     5521 gctgtggcgc cgatacatct tctggcgcag cttgtcgccg gtcagcttga ccttttccgc
     5581 gttgatcacc accacgtggt cgccggtgtc gacgtgcggc gtgaaaatgg gcttgtgttt
     5641 cccgcggagc aacaccgcgg cctccaccgc caaccgcccg aggatctgcc cctcggcgtc
     5701 gatcacgtgc caggccgata tgacgtccgc tttcttcgct gaatatgtcc gattcattgt
     5761 cctcatcccg cccgcgacga actgcgattc cacaaaggcc ctatttctac aaaatgcggc
     5821 atcccttgtc aagggaattc cgcgaaagat cctcgaattg gcctggctcg ccaacctgag
     5881 cgggcggacg gcgagcccat gattcgggta ccgcaagttg cggcttcgcg attttttgct
     5941 atcttccgag catgctccgc ttgctcgcca tcgcgttggt cggtctgttg ctggcggcct
     6001 gctcggggac cccgctcccc cgggaagcca agggccgccc ggggcttgac cttgacgcgc
     6061 ttcgagccga ctccgctcgg ccgatcagcg aaacgcgcca acgcgtgatc gccaccgcgg
     6121 ctcagcttgt cgggactccg tacaagtttg ggggaacaac accaaagggt ttcgactgta
     6181 ccgggtatct gatgtatgtc tttcggaagg ccgcgaacgt cgcgctcccg cgcacgtcca
     6241 tggagcagat ccaagccggc gatccgatcc cgccgtcgca gcttcaaccc gcggatctgg
     6301 tgtatttccg gatcgatcgc aagaaggacc tgcacctcgg tctgtacttg ggcgaaggcc
     6361 gcttcatcca cgcccccagc tcaggcggcg ctgtcaacat tcagcggctg gacatggagt
     6421 actggcgcac gcgcttcctg ggcgcacggc gcgtcctctc tctttagcaa actatttgct
     6481 ggcagagcct gttcagcggc ggccggatac aaggcgccgc gagaaccgga ccggagcgta
     6541 ctggttcgta cgtgaggacc ggaaagtgca ctgcgccgat tgccggcgct ggcaacggtc
     6601 ccttccgctc gcttggcgaa gcaagcgcag cggcaacgca gtagatggct gctgatggac
     6661 aggcgtctag cgcggaaacg cgtctttgac gccaccatca accggcaacg tcgcgccggt
     6721 ggtcttggcc gagcggttcg acgcgaagaa gagcacggct tcggccacgt cgtgcggcag
     6781 aatttttgcg cctagtagat tgcgtttcgc gtaaaagtcc tcgacctgct cgaccgagat
     6841 gccgtgcgcc ttggcgcgtt cggcgcggat ttcgggagac cagaggccgg agttgaggaa
     6901 caccgcgtcg gggttgacca tgttcacgcg gatgccgtgg cgcgcgttct caatggccat
     6961 gatcttggcc agttggtttt gcgcggcctt ggatgcggaa tacgcgccga agtcctggcc
     7021 cggggagagc acgttcttgg tggagatgac cacaatggat ccgccgagtc cttgggcccg
     7081 catgatgcgg atggcttcct gcgacgcgag caagtgcccc gtggcattga ccgccacgga
     7141 tcgctcccag tcggcctttg acaaccgatc gaccggcgcg cagtgcgcga tgcccgcgtt
     7201 ggacacgaag acgtcgagcc cgccgaaaca caatgccgtg tccgcgaacg cgcggcgcac
     7261 cgagcgctcg ttggtcacgt ccatttcgat cgccacggcg tggccgtcgc cgaaccgacg
     7321 gcaatactcc tccgtcaact tgcgcgcggc gtccagatcc acgtcggtca tggccaccat
     7381 ggcgccttcg gccagcaacc gctccgcgat tccgcggccg atcccgcccg ccgcgcccgt
     7441 gatcagcgcg attcgccgcg acagggattt ttccggcggc gcgagcgtga gtttgtagtt
     7501 ctccatgggc cagtattcaa aatcgcataa gtcgcgcggc ggcagcgggc gatagccgcc
     7561 cagagcgtgc gtggattgga tcacgcccat ggtgtgcagg tacagatcgc gggcgatgag
     7621 cgtctcgcgc cggttcttgc ccgtggtgaa catcccgatg ccggggatca atacgatgcg
     7681 cgggttggga tcaccctggg tcacgcccgg ggtcttgtat ttggtgaagt actccatgta
     7741 gcgcgtgcgg tagcgttcca atgccttggg cgcttcgacc agcaacgtcc gctcgtcgcg
     7801 cgcggctttg ggatccagcg ccaaaggcca cggcttggtg tagagcagat ggtccggcgt
     7861 gaacgggccg cagagcgttt tggggtccat gctccgggtg aacgccagca cttcgggccg
     7921 gtcgtcgtac aggagcacca cgggtttgcc gcggctcatc tcgccccgca acacgggcgc
     7981 gatcgcggcc gcggtttcgc ggcgatgcgc ggcggccgcg cgcggaatcg ccggagcccg
     8041 gcgcgcgcgg cgcggctgct tggatgcgat gtagcgctcg gccatgctca cgtatttgac
     8101 cgtgtccagg tacgcggcct tggcggtcgc gccccaggtg atcaggccgt gtttgtccag
     8161 gatcacggcg cgcgcgcgcg gatgcgcggc aaccgcctcg cccgtgcgtt tggacagcag
     8221 gaatcccggg cgcgagtacg gaaccaccac gatgtcgtcg cggtagagct cttcgatcac
     8281 gtcgcgactg ttcgcgatat cggtcagcgt gcagattgcg tccgcgtggg tgtgataaat
     8341 gtgcgcggcc gggatgaacg cgtgcaacag ggtttcgatc gacggtttgg gcgcacccgg
     8401 ctccagcgtg gacttggcct ggtacgcgac catttcctca tcggtcatcg cggcccggtg
     8461 aatcagcggc agcaggtctt ccaggcgcag cggggtgaac tgctgggccg tgatggtctt
     8521 catgtccgag ccgctgccct tgatgtacaa ggtgcgggtg ggccggccca cgtgatcctt
     8581 gcccatcacc ttgaccgagg agttgccccc tccccacaac accaggttcg ggctcgcgcc
     8641 gatcaggcgc gaggtgtaca ccaaggcatc caggccatcc agaccggacg cgtcgcgatc
     8701 agaccataga cttttcatgg catcgttttc agtggtacca gcgggtgagt aatgcgatcg
     8761 gatcgcggac cacgcggcac ctcacgcgcg ggccgtggct gcggttttca tttcccggac
     8821 cgcggcctcg attccgatca atgccgcgcg ggcgatcacg gcgtgaccca cgttgaactg
     8881 ggcgatctcg ggcacgggcg caaaagcgcg caccagatcc agggtgaggc cgtggcccag
     8941 ttgaaccggc atgccgactt tggccgccag tttggcggcc gtgacgatcc gctcctccca
     9001 cgcgcgccgc tccaacgggg tcttggctgc gcggtacgcg gtggcgttga gttccacgct
     9061 cagacccggt tggcccaatc ggtgacacgc tttcacgtcg tcgaccgacg gctggatgag
     9121 cagggtcacc gggatgccgg cctgcgcgag cgacgcggcc gcgttcgcca cgatctcgcg
     9181 cttgccgatc atgtccaatc cgccctcggt ggtcagttcc atgggatgct cgggcaccac
     9241 cgtgacccgc gccggacgga gttcgagcgc gaacttgagc atccggtcgg ccgcggaaat
     9301 ttccaaagtc aaccgggaag gcgccacgtg ctggaacccg tacagatcgc gatcttggat
     9361 gtggcggcga tcttcgcgca ggtgggcgat aagccaatcc gccccgccca tttccgccaa
     9421 caaggccgcg gccgcgacat tgggttcgat gccgccgcga acctgccgca gggtcgcgac
     9481 gtgatcaagt ttgagaccga gttgcgccat cccgtgccag tacgtccccg cgcgtgtgcg
     9541 cggcattgtg gcaccggcgc gggggaaaaa tcaagaaaac tcgggagatc gcggcgtaac
     9601 cggaggactg accggtcacc gtattgtgtt aagctgtggc cgagcggtcg aagagatttc
     9661 ttggcatgtt tcgcgtcgtc ttgcaccacc ccgagatccc
//
LOCUS       DBNGTT010000505         2218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2420505, whole genome shotgun sequence.
ACCESSION   DBNGTT010000505 DBNGTT010000000
VERSION     DBNGTT010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2420505"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="AC16BC_11685"
     CDS             <1..276
                     /locus_tag="AC16BC_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR3242320.1"
                     /translation="EQYGSLNEVVEALFASAEKNLYIQRYKGLGEMNPEQLWETTMDP
                     EKRTLLKVNPGDGVAADDMFTVLMGDEVEPRRAFIEKHAKEVKNLDI"
     gene            350..>2218
                     /gene="gyrA"
                     /locus_tag="AC16BC_11690"
     CDS             350..>2218
                     /gene="gyrA"
                     /locus_tag="AC16BC_11690"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3242321.1"
                     /translation="MPEQNVLINIEDEMRSAYINYAMSVIVGRALPDVRDGLKPVHRR
                     VLFGMNEMGLAWNRAYKKSARVVGDVMGKYHPHGDAAIYDTIVRMAQDFSLRYPLVDG
                     QGNFGSVDGDPPAAMRYTEVRMAQIGHELLADIDKETVDFGPNYDDTQTEPLVLPTRL
                     PNLLVNGAAGIAVGMATNIPPHNLNEVVDGLLLVLDNPSVTIDELMEVIKGPDFPTAG
                     FIYGKRGIGEAYRTGRGSLTLRARVRIETHPRTEKESLIVTELPYQVNKARLLEKIAE
                     LVSDRKIEGIADLRDESDREGMRIVIELKRGEIAAVILNQLFKHTSLQTTFGIISLAL
                     VRNQPQILNLHGMLSHFIEHRREVVVRRTRYELRKAEERAHILEGLKKAIDHLDAVIA
                     LIRAAADPEAAKTGLMTRFALSAIQAQAILDMRLQRLTNLEREKLVIEYQETLQKIES
                     LKAILASDALVRGVIREELAELKTKFGDERRTEIVEESAEISLEDMIAPEDVVITVSH
                     SGYIKRNAVSLYRAQRRGGKGKIGMGTKEEDFVTTLFSASTKDYLLFFTNSGKVYWLK
                     VHQVPEAGRVAKGKPIVNLLQLADGETVSAILPVSEFREDRYVVMATKNGTVKKTDL"
BASE COUNT          476 a          683 c          706 g          353 t
ORIGIN      
        1 gagcagtacg gctcgctcaa cgaggtggtc gaggcgttgt tcgcgagcgc cgagaagaac
       61 ctctacatcc agcgctacaa gggcctcggc gagatgaatc ccgagcagct ctgggaaacc
      121 accatggatc ccgagaagcg cacgctcctc aaagtgaatc ccggggacgg cgtggccgcg
      181 gacgacatgt tcacggtgtt gatgggcgat gaagtcgaac cccgccgcgc gttcatcgag
      241 aaacacgcca aggaagtgaa gaacctggat atctaatgtg gtatcggggc ggcccttgtg
      301 gccgcccagg gcaggcacga ggcctgcccc tacgaatcgt cggagaccta tgcccgaaca
      361 aaacgtgctg atcaatatcg aagacgagat gcgctccgcg tacatcaatt acgccatgag
      421 cgtgatcgtg ggccgcgccc tgcccgacgt gcgcgacggg ctcaagcccg tgcaccgacg
      481 cgtcttattc ggcatgaacg aaatgggact ggcgtggaac cgggcctaca agaaatccgc
      541 gcgcgtggtc ggcgacgtca tgggaaaata ccacccgcac ggcgacgccg cgatctacga
      601 caccatcgtc cgcatggcgc aagatttctc cttgcgctat cccctcgttg acggtcaggg
      661 caacttcggg tccgtggacg gggatccgcc cgcggccatg cggtacaccg aagtgcgcat
      721 ggcgcagatc ggccacgagc tgctcgcgga tatcgacaag gagaccgtgg acttcggtcc
      781 gaattacgac gacacccaga ccgaaccatt ggtgctgccc acgcggctgc ccaacctgct
      841 ggtgaacgga gcggccggca tcgcggtggg catggccacc aacatcccgc cgcacaacct
      901 gaacgaagtc gtggatgggt tgttgctggt gctggacaac ccgagcgtga ccatcgacga
      961 gttgatggag gtgatcaagg gcccggattt tcccaccgcg gggttcatct acggcaagcg
     1021 cggcatcggg gaagcgtacc ggaccggtcg cggatcgctc accctgcgcg cgcgcgtccg
     1081 catcgagacg cacccgcgca ccgagaaaga gagcctgatc gtcaccgaac tgccctacca
     1141 ggtcaacaag gcccggctgc tggagaagat cgcggagttg gtgtccgacc gcaagatcga
     1201 aggcatcgcg gacctgcgcg acgagtcgga tcgcgagggc atgcggatcg tgatcgaact
     1261 caaacggggc gagatcgcgg cggtcatcct caatcaactg ttcaaacaca cgtcgctgca
     1321 gaccacgttc ggcatcatct cgctcgcgct ggtgcgcaac cagccgcaga tcctgaacct
     1381 tcacggcatg ttgtcgcact tcatcgagca ccgccgcgaa gtggtggtcc gccgcacgcg
     1441 ctacgagctg cgcaaggccg aagagcgggc ccacatcctg gagggcttga agaaggcgat
     1501 cgaccatctc gacgcggtga tcgcgttgat ccgcgccgcg gccgacccgg aagcggccaa
     1561 gaccggcttg atgacccggt tcgccctgtc cgcgatccag gcgcaggcga ttttggacat
     1621 gcggctgcag cggctcacca atctggagcg cgagaagctg gtgatcgagt atcaagagac
     1681 cctgcaaaaa atcgaatccc tcaaagccat tctggcgagc gacgcgttgg tgcggggcgt
     1741 gattcgcgag gaactggccg agctgaagac caagttcggg gacgaacgcc gcaccgaaat
     1801 cgtggaggag agcgcggaga tctcgctgga agacatgatc gcgcccgagg acgtggtgat
     1861 cacggtctcg cactccgggt acatcaagcg caacgcggtg agcctctacc gcgcgcagcg
     1921 ccgcggcggc aaaggcaaga tcggcatggg caccaaggag gaggatttcg tcaccacgct
     1981 gttttccgcc tccaccaagg actatctcct cttcttcacc aattcgggga aggtctactg
     2041 gctcaaggtc catcaggtgc ccgaagccgg ccgggtggcc aaaggcaaac ccatcgtgaa
     2101 cttgctgcaa ctggcggacg gcgaaaccgt gtccgcgatt ctcccggtgt ccgagtttcg
     2161 cgaagaccgc tacgtggtga tggcgaccaa gaacggcacc gtgaagaaaa ccgatttg
//
LOCUS       DBNGTT010000506         5070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3187304, whole genome shotgun sequence.
ACCESSION   DBNGTT010000506 DBNGTT010000000
VERSION     DBNGTT010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3187304"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /gene="ccsA"
                     /locus_tag="AC16BC_11695"
     CDS             complement(<1..634)
                     /gene="ccsA"
                     /locus_tag="AC16BC_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR3242322.1"
                     /translation="MPVTVTATFHASTLVLYFSSAFLYLFDLNHHGRAVRWARTLVIV
                     AFAFNTVIFLVDWLGKGGMPPIGLREGFALLAWALALLFILMEWGYGVSSLGAFVVPV
                     IFFSAAAASIFHPGSREVVREHWNLWLNLHVVLVFLGHGAFALAATLGVMYLIQEKHL
                     KEKRFTTLFFRLGAVSELDTILHRTMVLGFPLLTLGMLSGAVYSRIAHGVF"
     gene            854..2005
                     /gene="metK"
                     /locus_tag="AC16BC_11700"
     CDS             854..2005
                     /gene="metK"
                     /locus_tag="AC16BC_11700"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR3242323.1"
                     /translation="MSRTNFLFTSESVTEGHPDKISDQVSDAILDAIIAEDPTCRVAC
                     ETLVTTGLAFVAGEITTDVYVEIPDIVRETIKDIGYTRAKYGFDYETCSVITSIHSQS
                     PDIAMGVDTGGAGDQGLMFGYATNETPEMMPMPIMLAHKLTRRLSEARRKDILPYLRP
                     DGKSQVTVEYKDGKPLRVETVVVSAQHSPDITLKEIREDIIEKVIKPVIPKAMLDEEK
                     ITYHVNPTGRFVVGGPQGDTGVTGRKIIVDTYGGVGSHGGGAFSGKDPSKVDRSASYM
                     ARYIAKNLVAAGVADRCEVQLAYAIGVADPVSVLVDTFNTGKIAQQKITKLVRDHFPM
                     TPRGIIEHLKLRRPIYKKTAAYGHFGRTEPEFTWERTDLADKIKKEAGL"
     gene            2050..3306
                     /gene="ahcY"
                     /locus_tag="AC16BC_11705"
     CDS             2050..3306
                     /gene="ahcY"
                     /locus_tag="AC16BC_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR3242324.1"
                     /translation="MDYDIKDIDLADKGRLRIEWAEQNMPVLRLIRKRFKKQRPLAGV
                     RVAACLHVTTETATLMETLKAGGADVALCASNPLSTQDDVAASLVAHAGIPVFAIKGE
                     DNKTYYKHLQSALAHRPNVSMDDGADLVSQLHGDWSHLAKDVIGGTEETTTGVIRLRS
                     MAKDGVLKFPVISVNDANTKHFFDNRYGTGQSTINGILRATNRLLAGSVFVVAGYGWC
                     GRGLAMRAHGQGANVIVTEVDPLKAIEAVMDGYRVMPMDDAAAIGDFFCTVTGNIKVI
                     GKEHFKVMKDGAIISNSGHFNVEIDIQALERLAKRKRVIRDFVEEYTMSDGRRINLLG
                     EGRLINLASAEGHPSSVMDMSFANQALSAEFVVKNAKKLKKDVYPVPAEIDSEIARLK
                     LNGMGINIDTLSKEQQKYLASWEMGT"
     gene            complement(3734..>5070)
                     /locus_tag="AC16BC_11710"
     CDS             complement(3734..>5070)
                     /locus_tag="AC16BC_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HIR3242325.1"
                     /translation="DLEHLSLFRDRFALEAALASTGRLDGERVIAAIGKGTPPRLRGL
                     LAVETGLAALTAALLTAAFVSGTPGYWILSLIAYGAVFWLATPHIAAVFSQAVDMESD
                     LAPYSRVFAVLERRSCRGRPALQQALAAFTSGNEPPSVSTRRLAGLVAALNLRGNPLV
                     HLLVNLALPWDLFFAERYRAFHGEIAQRLPLWIESLGRLEAASALAHYANTHPEFAYP
                     DIRLPRDLAAEVALDAVSVGHPLLPPDTRVTNSFALKGLGAVSIVTGSNMSGKSTFLR
                     TLGVNLCLAQAGAPVCAERWTSPWMRLYCCIRIDDSLDEGISYFYAEVKRLKRLLDAA
                     EDQNQPPVFFLIDEIFRGTNNRERLIGSRHYVRALGGANAIGLVSTHDLELVKLADEN
                     ARITTHHFRETVDERGLVFDYHLRPGPCPTTNALKIMAREGLPVPKESDPSS"
BASE COUNT         1055 a         1493 c         1666 g          856 t
ORIGIN      
        1 agaacacgcc gtgggcgatg cgggagtaga ccgcgccgct gagcatgccg agcgtgagca
       61 acgggaagcc gagcaccatg gtgcggtgca ggatggtgtc gagttcactc acggcgccga
      121 ggcggaaaaa caacgtggtg aagcgctttt ccttcaggtg cttttcctgg atgaggtaca
      181 tgacgccgag ggtggcggcc agcgcgaagg cgccgtggcc cagaaacacg agcaccacgt
      241 gaagattgag ccagaggttc cagtgttcgc ggacgacttc gcggctgccc gggtggaaga
      301 tcgaggccgc ggcggcggag aaaaaaatga ccgggaccac gaacgcgccc aggctgctga
      361 cgccgtagcc ccactccatc aggatgaaca acagcgccag ggcccaggcc agcagcgcga
      421 atccttcccg aagcccgatc ggcggcattc cgcccttgcc cagccaatcg acgaggaaga
      481 tgaccgtgtt gaacgcgaag gcgacgatca ccagcgtccg ggcccaccgc accgcgcgac
      541 cgtggtggtt caggtcgaac agatagagga aggcgctgga aaaatacaga acgagggtgc
      601 tggcgtggaa ggtcgcggtt acggtcaccg gcatgggcgg gatgatgcca gaagggtaaa
      661 aagattgtca aacaagggcc ggagcgcggt cgtcaacgcg ggaacatttt tttgttgcgc
      721 gaggtcacgg ctttttcata ttattggcgc acctttttcc ctcgactgga aggcgggaaa
      781 atcatctcgc agcatcgggc caaacacagg ccctgagctg cgttcagcgg gccttgaacg
      841 aaggagcggc attatgagtc gtacgaattt cctgtttacc tcggagtcgg tgaccgaagg
      901 acatccggat aaaatttccg atcaggtgtc cgacgcgatc cttgacgcca ttatcgccga
      961 ggacccgacc tgccgggtgg cgtgcgaaac gctggtcacc accgggctgg cctttgtcgc
     1021 gggggaaatc accaccgacg tctacgtgga gattccggat atcgtccgcg agacgatcaa
     1081 ggacatcggg tatacccgtg cgaaatacgg attcgattac gagacgtgtt cggtcatcac
     1141 gtcgattcac tcgcagtccc cggatatcgc catgggtgtg gacaccggcg gggccggcga
     1201 tcagggcctg atgttcggct acgccaccaa cgaaacgccc gagatgatgc cgatgcccat
     1261 catgctggcc cacaaactca cgcgccggct gagcgaggcg cggcggaagg acattctgcc
     1321 gtacctgcgt ccggacggga agtcccaggt gacggtcgaa tataaagacg gcaagccgtt
     1381 gcgcgtggag accgtggtcg tgtccgcgca gcacagcccc gacatcacgc tcaaagagat
     1441 ccgcgaagac atcatcgaga aggtgatcaa gccggtcatt cccaaggcga tgctcgatga
     1501 agagaaaatc acgtatcacg tgaatccgac cggtcgattc gtggtgggcg gtccgcaggg
     1561 cgacaccggc gtgaccggcc gcaagatcat cgtggacacg tacggcggcg tgggcagcca
     1621 cggcggcggc gcgttttccg gcaaggatcc gagcaaggtc gaccgttccg cgtcgtacat
     1681 ggcgcggtac atcgccaaga atctcgtggc cgcgggcgtg gcggatcgct gcgaagtcca
     1741 actcgcgtac gcgatcggcg tggctgatcc ggtctcggtc ttggtcgaca ccttcaacac
     1801 cggcaagatc gcgcaacaga agatcaccaa gttggttcgc gatcatttcc ccatgacgcc
     1861 gcgcggcatc atcgaacacc tgaagttgcg gcgaccgatc tataaaaaga ccgcagccta
     1921 cggccatttc gggcgcacgg agcccgagtt tacgtgggag cggaccgact tggccgacaa
     1981 gatcaagaaa gaagctgggc tgtaataccg gcctgacgac tcgtcgcata cacgaacgaa
     2041 ggagttcaga tggattacga catcaaggac attgacctgg cggacaaggg ccggctccgc
     2101 atcgagtggg ccgagcagaa catgccggtg ctgcggttga tccgcaagcg tttcaagaaa
     2161 caacggccgc tggcgggcgt gcgcgtggcc gcgtgcctgc acgtgacgac cgaaaccgcg
     2221 acgctcatgg agacgctcaa ggccgggggc gcggacgtgg cgctctgcgc gtcgaatccg
     2281 ttgtccacgc aggacgacgt ggcggcctcg ttggtcgcgc acgccggcat cccggtgttc
     2341 gcgatcaagg gcgaagacaa caagacgtac tacaagcacc tgcaatccgc gctggcgcac
     2401 cgtcccaacg tcagcatgga cgacggcgcg gacctcgtgt cgcagttgca cggagactgg
     2461 agccatctgg ccaaggatgt gatcggcggg acggaagaga cgaccaccgg cgtgatccgg
     2521 ctgcgcagca tggccaagga cggcgtgctc aagttccccg tgatctcggt caacgacgcc
     2581 aataccaaac acttcttcga caaccgctac ggtaccggcc agagcacgat caacggcatc
     2641 ctgcgcgcca ccaaccgcct gctcgcgggc tcggtgttcg tggtcgcggg ctacggttgg
     2701 tgcggccgcg gtctggccat gcgcgcgcac ggccagggcg cgaacgtcat cgtcaccgag
     2761 gtggatccgt tgaaggccat cgaagcggtc atggacggct accgcgtgat gcccatggac
     2821 gacgccgccg cgatcggcga cttcttctgc accgtgaccg gcaacatcaa ggtcatcggc
     2881 aaggagcatt tcaaggtcat gaaggacggc gccatcatct ccaactcggg ccacttcaac
     2941 gtggagatcg acatccaggc gttggagcgc ttggccaaac gcaagcgcgt gatccgcgac
     3001 ttcgtggagg agtacacgat gtcggacggc cgccgcatca atctgctggg cgaaggccgc
     3061 ctgatcaacc tggcgtcggc cgagggccat ccctcgagcg tcatggacat gagcttcgcg
     3121 aatcaggcgt tgtcggccga gttcgtggtc aagaacgcca agaaactcaa gaaagacgtg
     3181 tatcccgttc ccgcggagat cgattccgaa atcgcgcgcc tgaaactcaa cgggatgggt
     3241 atcaacatcg acacgctcag caaggaacaa cagaagtacc tggcgtcgtg ggagatgggc
     3301 acgtagccga attgtcagat tcgtggtgtt cgttcgtagg ggcgtatggc catacgcccc
     3361 gcacttcatg cctctcggta gccgtgttgg catgcggatt tatcatcacg atcccatgga
     3421 aatgattggg catcacgacg taggcgtcca attcgatctg cggtcgtata tcgggtgttc
     3481 gtaaccattc ttcttccacg atgcagccga gatcattcag gggggcattg aaaggccggg
     3541 cgtattgcag tacgccccta cgttccaaaa caaacattcc ctatctttgg tacagatagt
     3601 gaggaaatag gctccttctt gggagtaatc gaaatctttt aagcgaaccg atcggcggtg
     3661 gtgaatgtct gggttgtatg gatgaggcat ggggtgtatc gacaatgtgg gtggggcgta
     3721 tggcaacacg cccctatgac gacggatcgg actccttcgg caccggtagt ccttcgcgcg
     3781 ccatgatttt gagtgcgttg gtggtggggc aggggcccgg acgcaggtga tagtcgaaga
     3841 ccaggccgcg ctcgtccacg gtctcccgga agtggtgggt ggtgatgcga gcgttctcgt
     3901 ccgcgagttt gaccagctcc agatcgtgcg tgctcaccag gccgatcgcg ttcgccccgc
     3961 ccagcgcgcg cacgtagtga cggctgccga tcaaccgttc ccggttgttg gttccgcgaa
     4021 agatctcgtc gattaaaaag aacaccggcg gctggttttg atcctccgcg gcatcgagca
     4081 gacgcttgag gcgcttgacc tctgcgtaga agtacgagat gccttcatcc agcgagtcgt
     4141 cgatgcgaat gcagcaatac aaccgcatcc agggcgatgt ccagcgttcc gcgcacacgg
     4201 gagccccggc ctgggccagg cacaggttca cgcccagggt gcggaggaac gtgctcttgc
     4261 cggacatgtt ggagccggtc acgatcgaca ccgcgccaag acctttgagc gcgaacgagt
     4321 tggtcacgcg cgtgtcgggc ggcaggagcg ggtgtccgac cgagaccgcg tcgagcgcga
     4381 cttcggcggc aaggtctctc ggcaaccgaa tgtccgggta cgcgaattcg gggtgcgtgt
     4441 tcgcgtagtg cgccagcgcg gaggcggcct caaggcggcc caggctctcg atccaaagcg
     4501 gcaggcgttg cgcgatctct ccgtggaacg cgcgataacg ctccgcgaag aacaggtccc
     4561 agggaagcgc gaggttgacg agcaggtgca ccagcggatt gccgcgaaga ttcaacgcgg
     4621 cgacaagccc cgccaaccga cgcgtggaca ccgagggtgg ctcgttgccg gaggtaaagg
     4681 ccgcgagcgc ctgttgcagc gcggggcgcc ctcggcatga ccggcgttcc aggaccgcaa
     4741 acacgcggga gtacggcgcg aggtccgatt ccatgtccac ggcctgcgag aagaccgcgg
     4801 cgatgtgcgg cgtggccagc cagaagaccg cgccgtaggc gatcagggag agtatccagt
     4861 accccggagt cccgctgacg aacgcggcgg tcaacagcgc ggcggtcagc gcggcgagtc
     4921 cggtctcgac ggccaagagg ccgcgcaggc ggggcggcgt gcccttgccg atcgccgcaa
     4981 tgacccgctc gccgtcgagt cgtccggtcg acgccagcgc ggcttccagc gcgaaccggt
     5041 cgcgaaacag cgacaagtgt tccaagtctc
//
LOCUS       DBNGTT010000507         1955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_250382, whole genome shotgun sequence.
ACCESSION   DBNGTT010000507 DBNGTT010000000
VERSION     DBNGTT010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_250382"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /gene="gspC"
                     /locus_tag="AC16BC_11715"
     CDS             <1..609
                     /gene="gspC"
                     /locus_tag="AC16BC_11715"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HIR3242326.1"
                     /translation="PVVLVRTGTAEGAGSYAILEDRGTREQQVYRLHDVVRDGAVLAK
                     VERNQAVLRLGNQEQVLTIYNDGTQDVAPPSNQGFRATPDAEASPAGIRQVQPNQWVL
                     DKQEVTAALGNLPQLLTKARVIPNFADGKPDGFKIFSIAPDSLYAKIGLQNGDVLQQI
                     NGVEIKDPENFMRVFQQLKDESRIALDLVRNNRRESYTYEIR"
     gene            778..1875
                     /locus_tag="AC16BC_11720"
     CDS             778..1875
                     /locus_tag="AC16BC_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR3242327.1"
                     /translation="MAAAILLAATPAFAQDPGPGTVAATPASAPSGKISLDFKDVDLP
                     VLVKFFSELTQRNFVIDEKVRGKVTIFSPSKLSIDDAYRVFLSVLELKGFAAVPSGEV
                     TQILPVAEVPADRVVEVYPLAHANAEDLVKVLNVVVARPAQAGPARPGLRSAGDFEGP
                     IQVLSDKPTNSLIVTGTARDLEILKDIVRKLDTARRQVYVEAVIMEVSVARLKELGSD
                     VTSLFGMQNDSKRLSAVAGFNEAPPTFEEVASLDFTKISAFNVRAVLRALQTRDDVNI
                     LSTPQILTSDNQKAEIVVGENRPFPTGQSQTSGGNTITTIERRDVGITLRLTPQILES
                     NQVKLDVFQEISSVSSESQTAGSVPLGVVTN"
BASE COUNT          379 a          600 c          634 g          342 t
ORIGIN      
        1 ccggtggtgt tggtcagaac gggaacggcc gagggcgccg gatcgtacgc gattctcgag
       61 gatcggggga cacgcgagca gcaggtgtac cgtctccacg acgtggtgcg ggacggcgcc
      121 gtgctcgcaa aagttgaacg caatcaggcc gtgttgcggt tgggaaatca ggaacaggtg
      181 ttgacgatct ataacgacgg gacccaggat gtggccccgc cgtcgaacca ggggttccgc
      241 gcaacgccgg atgcagaggc ctctccggcc ggcatccgcc aggtgcagcc gaatcaatgg
      301 gtgctggaca aacaagaggt gacggccgcg ttgggcaacc tgccccagct cctcaccaaa
      361 gcgcgggtga ttccgaattt tgccgacggc aagcccgacg ggttcaagat tttctcgatc
      421 gcgccggaca gcttgtacgc gaagatcggc ctccagaacg gcgacgtgtt gcagcagatc
      481 aacggggtgg agatcaagga cccggagaac ttcatgcggg ttttccaaca actcaaggac
      541 gagagtcgga ttgcgctcga tcttgtgcgg aacaatcgac gcgagagtta cacctatgag
      601 atccgttgaa ccgttggaca tcgtcggccg ccccggcaga tcgggtcgtc cccgccgcgg
      661 atcttgccac cagcgagcgg cgtgacgcgt cgcatgacga gacggggccc acgccctcgc
      721 agtcggtttc cgtcggtctt ccctcacccg cgaggtcttt tcggcatcca gggcggcgtg
      781 gccgcggcga tcctgctggc ggcgaccccg gcgtttgccc aggaccccgg tcccgggacg
      841 gtggcggcga ccccggcgag cgcgccgtcc ggaaaaatct cgctcgactt caaagacgtc
      901 gacctcccgg tactggtcaa gtttttttcc gagctcacgc aacgaaattt cgtgatcgac
      961 gagaaagtgc ggggaaaggt gaccatcttt tccccgtcca agctgtcgat cgacgacgcc
     1021 tatcgcgtgt ttctatccgt gctcgaactc aagggattcg cggcggtgcc gtcaggcgaa
     1081 gtcacgcaaa tcctcccggt ggcggaggtt ccggcggatc gcgtggtcga ggtgtacccg
     1141 ctggcgcacg ccaatgcgga agacctggtc aaggtgctca acgtcgtggt cgccaggccg
     1201 gcgcaggccg gtccggcgag gcccggtctg cggtcggccg gggactttga aggtcccatc
     1261 caagtcctgt ccgacaagcc gaccaattcg ctgatcgtca ccgggacggc gcgcgatctc
     1321 gagatcctga aggacatcgt gcgcaaactg gacaccgcgc ggcgccaggt ctacgtcgag
     1381 gcggtaatca tggaggtgtc ggtcgcgcga ttgaaggaat tgggcagcga cgtgaccagc
     1441 ttgttcggca tgcagaacga cagcaagcgg ctgtcggccg tggccggttt caacgaggcg
     1501 ccgccgacgt ttgaggaggt cgcgtccctc gactttacga aaatcagtgc gttcaacgtc
     1561 cgggccgtgc tccgcgcgct ccagacacgc gatgacgtga acatcctgtc tactccgcaa
     1621 attctgacca gcgacaacca gaaggccgag atcgtggtgg gtgaaaatcg tccgttcccg
     1681 accggccaga gccaaacctc aggcggcaat acgatcacaa ccattgagcg gcgcgacgtg
     1741 ggcatcacgc tccggctgac tccgcagatt cttgaaagca accaggtcaa gctggacgtg
     1801 tttcaggaaa tttccagcgt gtccagcgag tcccagaccg ctgggtccgt cccgctgggc
     1861 gtcgtgacca actaacgctc cgccacgacc tccgtgctcg tggccgacgg gcagaccgcc
     1921 gtgatcggcg gattgattcg cgacaacgtg atcac
//
LOCUS       DBNGTT010000508         3928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1655568, whole genome shotgun sequence.
ACCESSION   DBNGTT010000508 DBNGTT010000000
VERSION     DBNGTT010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1655568"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2059
                     /gene="uvrA"
                     /locus_tag="AC16BC_11725"
     CDS             <1..2059
                     /gene="uvrA"
                     /locus_tag="AC16BC_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3242328.1"
                     /translation="GACPSCDGLGVTRVFDLARVVPDPSRSLREGAIRPWERRTSGYY
                     EELLEAVARHYGFSVRTPFGELPPAHRQVVLYGSGEETVECWFEKSGRRQTYQRPFEG
                     VIPNLDRRYRETTSPVLRAEFEQYMSEQPCPACRGARLKPESLAVKVGGLSIAEVTRL
                     SIRGADRFIRDLRLGEQAALIAQRILKELRERLGFLVNVGVDYLALDRSAATLSGGEG
                     QRIRLATQIGASLVGVLYVLDEPSIGLHQRDNRRLLDALTRLRDLGNSVLVVEHDEET
                     ILAADHVIDMGPGAGVHGGTVVAEGPPAAIMAHPASLTGRYLTRELAVGLPPRIRPTE
                     RCLTIVGATEHNLKDLTVPIPLGLFTCVTGVSGSGKSTLIIDILYKTLAQRLYRAADR
                     PGACRAITGAETLDKVVNVDQSPIGRTPRSNPATYTGVFALIRDLYTQLPESKARGYK
                     AGRYSFNVKGGRCESCQGDGVIKIEMHFLPDVYVTCDACHGRRYNRETLDVRYKDRSI
                     ADVLDMTVEQALEFFAAIPAIRQKIETLRDVGLDYARLGQSATTLSGGEAQRVKLAKE
                     LSRRATGNTVYILDEPTTGLHFADIQRLLDVLNRLVESGNTVVVIEHNLDVIRNADHV
                     IDLGPEGGDGGGELVASGSPEEVSRIARSLTGRFLARTPQAGPNRTGRKMTRRPIDHQ
                     PLA"
     gene            complement(1958..2977)
                     /locus_tag="AC16BC_11730"
     CDS             complement(1958..2977)
                     /locus_tag="AC16BC_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3242329.1"
                     /translation="MAKATPDGALVAAIDLGTNAFRLLVADTGPDGCVNARHVHRVFP
                     RLGEGVGRTGRLSQPAITRSMAALEEIRSVMARFPVDAVVAVATSAVREAANAAEFLD
                     RAKTDAGLDVEVISGGEEARRTWLGVRAGLAASGRAVGDAVVMDIGGGSTELISVRGG
                     EFHHAMSLDLGVVKLTERCVAHDPPRGDELRRLDDEAREALSEALPVREASGPRPAVI
                     GTAGTVTAAAVLHLGLTRYEPALIHDRVIPRSAVRDVAARLSRMTADERRRLPSLDRG
                     REDVILAGLAILGEALDFLGAEDVRVSEYGLREGLVIDWAARHLASRAVRSGLGRSRE
                     KPTGQ"
     gene            complement(2978..3382)
                     /locus_tag="AC16BC_11735"
     CDS             complement(2978..3382)
                     /locus_tag="AC16BC_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242330.1"
                     /translation="MPEVPEGGIWPAPEQAPTLVCPDPWGDGLMVDVWRKIRDDGSTG
                     LLAMLSGVAGAIRGGGSMAEVLRQRWRLRETREQLGDAYTELGRHVADALTAGGSVEM
                     TDEQARRLCQRIDDLLAVERDVRDELTRGNDS"
     gene            complement(3686..>3928)
                     /locus_tag="AC16BC_11740"
     CDS             complement(3686..>3928)
                     /locus_tag="AC16BC_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3242331.1"
                     /translation="IAERDLAARGPGEFLGTRQSGLPDLKVANLIRDARLVELARREA
                     FAVFDRDPTLSAPEHAPLKAALKRTWGRRLALASIG"
BASE COUNT          665 a         1424 c         1233 g          606 t
ORIGIN      
        1 cggcgcgtgc ccgtcctgcg acggcctcgg cgtgaccagg gtgttcgatc tcgcgcgcgt
       61 ggtgcccgac ccctcgcgtt cgttgcgtga gggcgcgatc cggccgtggg agcgccgcac
      121 gtcgggctac tacgaggaac tcctggaggc cgtggcccgg cactacggat tttccgtccg
      181 caccccgttc ggagagttgc cccccgccca ccgccaagtc gtcctatacg gttcgggcga
      241 ggaaaccgtg gagtgctggt ttgagaaatc cggccgtcgg cagacctacc aacgtccgtt
      301 cgaaggcgtg atccccaatc tggaccgccg gtaccgggaa accacgtcac cggtgctccg
      361 cgcggaattc gagcagtaca tgagcgagca gccatgtccc gcctgccgcg gggcccggct
      421 caagccggaa agcctggcgg tgaaggtcgg cggcctttcc atcgccgaag tcacccgctt
      481 gtccatccgc ggcgcggacc gattcatccg cgacctcaga ttaggcgaac aggccgcgct
      541 gatcgcccag cggattctca aggaactgcg cgaacgcctg gggttcctgg tcaacgtggg
      601 cgtcgactat ctcgccctcg accggtccgc ggccacgttg tcgggcggcg aaggacagcg
      661 gatccgactc gccacgcaga tcggcgcgtc gctggtgggt gtgctgtacg tgctcgacga
      721 accttcgatc ggactgcacc agcgcgacaa ccgtcgactc ctcgacgccc tcaccaggct
      781 ccgcgacctg ggcaactcgg tgctggtggt cgaacacgac gaggaaacga tcctggccgc
      841 ggaccacgtc atcgacatgg gacccggcgc cggcgtgcac ggcggcaccg tggtcgccga
      901 gggcccaccc gccgcgatca tggcccaccc cgcctcgttg acgggccgct atttgacccg
      961 cgaactcgcg gtcggcctgc cgccccggat ccgtcccacg gaacgatgcc tcaccatcgt
     1021 aggcgccacc gaacacaacc tcaaagacct caccgtgccc attccgctcg gcctgttcac
     1081 gtgcgtcacc ggcgtctcgg gatcgggcaa gagcacgctg atcatcgaca tcctctacaa
     1141 gacgctcgcg caacgcctgt accgggccgc cgaccgaccc ggggcatgcc gcgcgatcac
     1201 cggggcggag acgttggaca aggtggtcaa cgtggatcaa tccccgatcg gccggacccc
     1261 ccgatccaac cccgcgacct acaccggcgt gttcgcgttg attcgcgacc tgtacaccca
     1321 actcccggaa tccaaagccc gcggctacaa agccggccgg tattccttca acgtcaaggg
     1381 cggacgctgc gagtcgtgtc agggcgacgg cgtgatcaag atcgagatgc acttcctccc
     1441 ggacgtgtac gtgacctgcg acgcctgcca cggccgccgg tacaaccgcg aaaccctcga
     1501 cgtgcgatac aaggaccgct cgatcgccga cgtcctggac atgaccgtgg agcaagccct
     1561 ggagtttttc gcagcgatcc ccgcgatcag acagaaaatc gaaaccttgc gggacgtggg
     1621 gctggactat gcgcggctgg gccagtccgc caccacgttg tccgggggcg aagcccagcg
     1681 cgtgaaactc gcgaaggagc tctcccgccg ggccaccggg aacacggtgt acatcctgga
     1741 cgagccgacc acggggctac acttcgcgga catccaacga ctccttgatg tcctgaaccg
     1801 gttggtggaa tccggcaata cggtggtggt gatcgaacac aacctcgacg tgattcgcaa
     1861 cgcggatcac gtcatcgacc tgggcccgga aggcggcgac ggcggcggcg agctcgtggc
     1921 cagcggctcg cccgaggagg tctcgcgcat cgcccgctca ctgaccggtc ggtttctcgc
     1981 gcgaacgccc caggccggac cgaaccgcac gggacgcaag atgacgcgcc gcccaatcga
     2041 tcaccagccc ctcgcgtaac ccgtattcgc tcacccggac gtcctcggcc ccgagaaaat
     2101 cgagcgcttc gccgaggatg gcgaggcccg cgagaatcac gtcttcccgc ccccggtcca
     2161 acgacgggag tcggcggcgc tcatccgcag tcatgcgtga caggcgcgcg gcgacatccc
     2221 tgaccgcgga gcgcgggatc acgcgatcgt ggatcagggc gggttcgtat cgcgtgagtc
     2281 ccaggtgcag gaccgcggcg gcggtcacgg tgcccgcggt cccgatgacc gcggggcgag
     2341 gccccgaggc ctcgcggacc ggcaacgcct cgctcaaggc ctctcgagct tcgtcgtcga
     2401 gccgccgcag ttcgtcgccg cgcggcggat catgcgccac gcaccgctcg gtcagcttca
     2461 ccacgccgag gtccaggctc atcgcgtggt gaaactcgcc cccgcgcacg ctgatcagct
     2521 cggtgctgcc gcccccgatg tccatgacca cggcgtcgcc caccgcgcgg cccgacgcgg
     2581 cgagacccgc gcgaacgccc aaccacgtcc gtcgcgcttc ttcgcctccg gaaatgacct
     2641 cgacgtcgag gcccgcgtcc gttttggcgc ggtcgagaaa ctccgcggca ttggccgcct
     2701 cgcgaaccgc gctcgtggcg accgccacca ccgcatcaac cgggaaccgg gccatgacgg
     2761 aacggatttc ttccaacgcg gccatcgacc gggtgatggc gggttgactc aaccgcccgg
     2821 ttcggcccac gccctcgccg agtcgcggga acacccggtg cacgtgacgc gcgttcacgc
     2881 atccgtcggg ccctgtatca gccaccagga ggcggaacgc gttcgtcccc aaatcgatcg
     2941 cggcgaccag cgcgccgtcc ggcgttgcct tcgccattca ggagtcgttc ccgcgcgtca
     3001 gttcgtcgcg cacgtcgcgt tccacggcca acaggtcgtc aatgcgctga cacaaccgac
     3061 gcgcctgttc atcggtcatc tccaccgagc caccggcggt cagcgcgtcg gccacatgtc
     3121 tgcccaactc ggtgtaggcg tcccccagtt gttcgcgcgt ttcccgcagg cgccagcgct
     3181 gccgcaagac ctcggccatc gatcctccac cccgaatcgc gcccgcgacc ccgctgagca
     3241 tcgcgagcag cccggtcgag ccgtcatcgc ggattttgcg ccacacgtcc accatcaatc
     3301 catcccccca agggtccggg cacaccagag ttggcgcctg ttcaggagcg ggccagatgc
     3361 ccccttcggg gacttccggc attcgaactc tcgaaggagg gtcgcaaggc ggaatctgcc
     3421 ccgcgccgtc gggcgcggag tgcacttgtt ccatccgctc agatggcgaa gcagcgagcg
     3481 cccgcaccgc agcgtactgg gtcgtatgtg aggaacggaa agcgcactgc gccgttcttt
     3541 ggcgctgtca atgctacggt ccgctcgctt ggcgaagcac gcgcagcgac aacaccgcag
     3601 atggtcgctg ctgtgccacc tgtagagagc ctgcggtcga attatcactt gcgccttcgg
     3661 cgcatccagg ttcatggaca ggctcctatc cgatcgaggc cagcgccaaa cgccgccccc
     3721 acgtccgctt gagcgcggcc ttcaagggcg cgtgctccgg cgcggacagc gtggggtcgc
     3781 gatcgaacac cgcaaacgct tcccggcgtg cgagttccac caatctggca tcgcgaatca
     3841 agttcgcgac cttgaggtcg gggagccctg attggcgcgt ccccaggaat tcgccggggc
     3901 ctcgggcagc cagatcccgc tccgcaat
//
LOCUS       DBNGTT010000509         9880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2676691, whole genome shotgun sequence.
ACCESSION   DBNGTT010000509 DBNGTT010000000
VERSION     DBNGTT010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2676691"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2579
                     /locus_tag="AC16BC_11745"
     CDS             <1..2579
                     /locus_tag="AC16BC_11745"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0050667 - homocysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR3242332.1"
                     /translation="GTTPAHIAAIAQMVAGKQPRVPKTSLRPAVSGIDYLPFEEDNRP
                     LIVGERTNVIGSRKFKDLIVDGKIEEASEIGRAQVKNGAQIVDVCMANPDRNELADMD
                     AFLAVLTKKVKAPLMIDSTDHHVIEAALTRCQGKCIINSINLEDGEERFEQVVPLIKR
                     YGAAVVVGCIDEDPKQGMAVTRDRKLAVAKRSYDLLVNKYGLPPRDLIFDALVFPVGT
                     GDENYIGSAVETIEGIKLIKAEFPQCRTILGVSNVSFGLPSAGREVLNAVYLYHNVKS
                     GLDLAIVNAERLERYPSIPEEERRLAEDLIFWRGADPVAAFAAHFSGRPAKVRAPKTS
                     LPLDERLARYIVEGSRDGLVDDLNEALTTRAPLAIINGPLMAGMDEVGRLFNNNELIV
                     AEVLQSAEAMKAAVNHLEPFMEKTESSSKGKILLATVKGDVHDIGKNLVEIILGNNGY
                     RIVNLGIKVAPEALIEAYRREKPDAIGLSGLLVKSAQQMVVTAQDLKNAGIDAPILVG
                     GAALSNKFTATKIAPEYSGVVLYAKDAMMGLDLANRLSNADERQSLIARIREEQRSLV
                     AGSAPAKPVTAPSAPVAARSAVRRDTPVPPAPDFDRHVIRDAPLGEIFAYINPIMLYG
                     KHLGLRGQIDKLLADKDEKAIKLHDTVEGLKREIMEHKLLSAHAAWRFFPCQADGDDV
                     ILYDPANPDRAAERFHFPRQSAGERLCLADYVRDTASGERDAVALFAVTCGVGVRELS
                     QRWKDAGEYLRSHALQAIAVEAAEGMAEWLHRRLRTAWGFPDPPTTTIHDVLKNKYRG
                     IRVSFGYPACPNLADQRKLFALIRPQDIGVELTEGDMMDPEASVSALVFHHPEAKYFR
                     AD"
     gene            2678..2995
                     /locus_tag="AC16BC_11750"
     CDS             2678..2995
                     /locus_tag="AC16BC_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242333.1"
                     /translation="MLWSTGAWRSLFSVCLLIAYAVLVGDAIHCQYSSVDHDHHDVPK
                     PQSAENTTHCLVANHGSAALPTIAPGSLPGLHSEQYRLGSVALPLLSSAFVVTASRAP
                     PRL"
     gene            3118..4047
                     /locus_tag="AC16BC_11755"
     CDS             3118..4047
                     /locus_tag="AC16BC_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242334.1"
                     /translation="MPRYGLGLLYVALTLFVVSVHGSAWGHGVVGQRFFPESLSVEDP
                     FPSDEADFPAISFIKGPEGKERAVGLEYSKRLSPNLAFGFEVEHVSLSPADPTEPRQS
                     GLNNPELFLKYAMLRDPEHEAIVSVILSVAPPWGDKDFTERNTTVAPGLIFGKGLGGL
                     PDGLRYLKPIALGGAVSLETPLGNITDPVDQTSTLNYGFIVEYSLPYLQSFVKDIGLS
                     KPFSRMIPIVEFSMATPVNGPEHGATTAYVNPGLIWAGKYVQFGAEALIPLNDRTGHN
                     VGVLAVLHWYLDDLAPNAFTWTPFHGTLGPTRK"
     gene            4074..4436
                     /locus_tag="AC16BC_11760"
     CDS             4074..4436
                     /locus_tag="AC16BC_11760"
                     /inference="COORDINATES: protein motif:HMM:NF016149.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HIR3242335.1"
                     /translation="MAAGGILTGLLLFSSSMAWAHAFPDHSEPRVGSDLKAPPSSVKI
                     WFDSELEPLFSTIEVFDAQQHKVDNGDGGVNAKDHSLLEVTVSSLAPGAYTVSWRVVS
                     HDGHPTEGRFPFTVSGAR"
     gene            4433..5575
                     /locus_tag="AC16BC_11765"
     CDS             4433..5575
                     /locus_tag="AC16BC_11765"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance D family protein"
                     /protein_id="HIR3242336.1"
                     /translation="MSLLDPHMLLHLVVRALDLAAMVIVVGGLAFDAFVMTPVLGRLD
                     LSPSERAPVDAAYRARYTRLVGWALAVLVIIQFADLLLRVQMMSGKPFSVVMGLLPSA
                     ITGTHIGKVWIAKIGALTILAIVWLVTYRRSAAPSGGSWNRPGLLAIAAIVSIMVALA
                     GHAADQGNVSPDVAADWLHVLSICTWVGGLVAFMVLVPTASRVAGERHAARLFTRAMA
                     RFSTVAGWSLGALLLTGAYGVWLHIHSWAGLAGAYGTALLVKLAFVAPMIGLGALGRF
                     DNLPELQTMTGEAFRSTWLARVAAASFATIRRLSGVDPLRATHETIAAVRSRSLRFIV
                     WECVFAVGVLAGTSVLTQTMPPHVTDFAVPEASPHHMMDMPNGSMP"
     gene            5614..5955
                     /locus_tag="AC16BC_11770"
     CDS             5614..5955
                     /locus_tag="AC16BC_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242337.1"
                     /translation="MKLRLPQPARFTWRIAVSAVLMVGYLALMGESIHCQYVADAQPE
                     HHHSSSSAPPVNHQTHCLAANHGATATIHSVVLPPIDVPNQVGFLLTKNQTVPDSRFI
                     APGTARAPPAV"
     gene            6164..7084
                     /locus_tag="AC16BC_11775"
     CDS             6164..7084
                     /locus_tag="AC16BC_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR3242338.1"
                     /translation="MTGTQEGIANPGQVIADLSYRFIPMDQVQRGSSGAPDAVVPKID
                     FTNNQIIQPGDIDSHHEIRTNNELMQLDLTFGITPKFALTLAVPFFNQRTHEHEHVTG
                     GTPSNPTFGSFSREDGTAGFGDVRVIGKYALLIHTKHLLVGGLGIKAPTGEYKLRDHE
                     GEINEPTIQPGTGSWDGTASLYYAYEIFPHELNTFASTSYQANTDNDLHYQMGNTLLI
                     NAGVSWLASEKVNLSAQINMRQAPHDSFNDETVPSTGGRWVYFTPGVRFQGAPDTALY
                     AHVQIPIYQYVNEVNIVPRYGFIMGISRAF"
     gene            7133..8581
                     /locus_tag="AC16BC_11780"
     CDS             7133..8581
                     /locus_tag="AC16BC_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3242339.1"
                     /translation="MKTRVSTPARLGAYLLATVTVVGCGQAAVAPSANNAASTGLEAL
                     VFVGNNGNGTVSVISHGHDGNAVLNTIPVGSGAVGDINISSERHVFVNVTANNQVAAI
                     DPVVTGDPVLKNFLPVGQKPVHGYRDPSDGTLIWVLNDANATSGTCATARSGAATNSV
                     TVIQNHEVGGGGGGGSGGGQSTLGIVLKEICVGRGHHKAAFSYPSPTHQGVPRRAFVS
                     NISDGTVTVIDSDPASATFGDVVGTVDLCDSAKQTCDTDPTTGNGAVPHGIDFSPVSG
                     LVYNANVGYGTVWAIDPITPALVDVNPATPEVDPINVGYANKSHVSPDGRFLVVKGTD
                     TASNADHVIGKLSVVDVSDHSVHQIDLMDIHPDSFEFSPDGTKLYVTTATSVASGNTV
                     QPTTIKNNVLLSFNASALPQLPSPIEIPVGVAGHDHRSLAIHEHNGAAEHVWVPNPDD
                     GTVSVVDAESDTVVDTVTVGGEPSSIAVFAIE"
     gene            8604..9164
                     /locus_tag="AC16BC_11785"
     CDS             8604..9164
                     /locus_tag="AC16BC_11785"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR3242340.1"
                     /translation="MHTRHDHSAGGAGAKAPARFLLATLLFVAGAGAAATQAHAAPPQ
                     IGKPAADFSLLDTTEQNVRLSALRGRPVLLTFWATWCEPCKIEMPEIQKAYERFKTDG
                     FVVLAVNFGEKAEKARAYAETNGLTFPVLVDRRANVASQYSVVSLPVSLFIDADGVVQ
                     ERVFGGTLTLERIAQVLHRLRQTSDQ"
     gene            9433..>9880
                     /locus_tag="AC16BC_11790"
     CDS             9433..>9880
                     /locus_tag="AC16BC_11790"
                     /inference="COORDINATES: protein motif:HMM:NF027967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3242341.1"
                     /translation="MRRSLEPVMARYRTLARSAAAALLLLSGCGGGGDSGGGGGGTPS
                     DTSPPIFSGVTVGVGTLTSDQATVTWNTDEPSTSQVLYGITRAYASSSTLDSTLVTSH
                     SVTLYNLTPSTTYYLKAKSIDSAGNPNTSIEVIFTTPSSYFIVTTDT"
BASE COUNT         1834 a         3223 c         3066 g         1757 t
ORIGIN      
        1 gcggcaccac ccccgcgcac atcgcggcga tcgcccagat ggtcgctggg aaacaacccc
       61 gcgtacccaa gacctcgttg cggcccgcgg tgtccggcat cgactacctg ccgttcgaag
      121 aggacaaccg tccgctcatc gtgggcgaac gcaccaacgt gatcggcagc cgaaaattca
      181 aggacctgat cgtcgacggc aagatcgagg aggcatccga aatcggccgc gcgcaggtca
      241 aaaacggcgc gcagatcgtg gacgtgtgta tggccaaccc ggatcgcaac gagctggcgg
      301 acatggacgc gttcctcgcg gtgctgacca agaaggtaaa ggcgccgctt atgatcgact
      361 cgaccgatca ccacgtgatc gaggccgcgc tcacgcgttg ccagggtaaa tgcatcatca
      421 actcgatcaa cctggaggac ggcgaggagc ggttcgagca ggtggtgccg ctgatcaagc
      481 gttacggtgc cgcggtggtg gtggggtgca tcgacgaaga ccccaaacag ggcatggcgg
      541 tcacgcgcga tcgcaagctg gccgtggcca agcggtcgta cgatctcttg gtcaacaagt
      601 acggcctgcc gccgcgggac ctgatctttg atgccctggt gtttcccgtg ggcacgggcg
      661 acgagaacta catcggctcg gccgtggaga cgatcgaagg catcaagttg atcaaggccg
      721 agtttcccca gtgccggacc atcctcggcg tcagcaatgt atccttcggc ctgccgtccg
      781 cgggtcgcga ggtcctgaac gccgtgtatc tgtaccacaa cgtcaaatcc gggctcgatc
      841 tcgcgattgt caacgccgaa cggctggagc ggtacccctc gattccagag gaggagcgcc
      901 gcctcgccga ggatttgatc ttctggcgcg gcgcggaccc ggttgcggcg ttcgccgcgc
      961 acttcagcgg caggccggcc aaggtccgcg cgccaaagac ttcgctgccg ctggatgaac
     1021 gcctggcgcg gtacatcgtc gagggctcgc gcgacgggct ggtggacgat ctcaacgaag
     1081 cgctcacgac ccgcgcgccc ttggccatca tcaacggccc gctcatggcc ggcatggacg
     1141 aggtgggccg gctgttcaac aacaatgagt tgatcgtggc cgaggtgctc caaagcgcgg
     1201 aggccatgaa agccgcggtg aaccacctgg aacctttcat ggagaagacg gaatcctcca
     1261 gcaagggcaa gattctgctc gccacggtca aaggcgacgt gcacgacatc ggcaaaaacc
     1321 tggtggagat catcctgggc aacaacggct accggatcgt gaacctgggc atcaaggtcg
     1381 cgcccgaagc gctcatcgag gcgtatcgcc gcgagaaacc ggacgcgatc ggcctctccg
     1441 gcctgctggt gaaatccgcg cagcaaatgg tcgtcaccgc gcaggatctc aagaacgcgg
     1501 gcattgacgc gccgatcctg gtcggcggcg cggccttgtc caacaagttc accgcgacca
     1561 agatcgcgcc cgagtattcc ggggtcgtgc tctatgccaa agacgcgatg atggggctgg
     1621 acctggccaa ccggctcagc aacgcggacg aacggcaaag cctgatcgcg cgcatccggg
     1681 aggaacaacg atccctcgtg gcgggctccg cgccggccaa acccgtcacc gcgccgtcgg
     1741 ccccggtcgc ggcccgatca gccgtacgcc gtgacacgcc cgtacccccg gcgccggact
     1801 tcgaccgcca cgtcatccgc gacgcgcccc tgggcgagat cttcgcctac atcaacccga
     1861 tcatgctgta cggcaaacat ctggggttgc gcggtcaaat cgacaagttg ctcgcggaca
     1921 aggacgaaaa agcgatcaag ctccacgaca cggtggaagg attgaagcgc gagatcatgg
     1981 aacacaagct gctgtcggcg catgcggctt ggcgcttctt tccctgccag gccgacggcg
     2041 acgacgtgat cctctacgac ccggcgaatc ccgaccgcgc ggcggagcgg tttcattttc
     2101 cccgacagtc cgcgggcgaa cgcttgtgcc tggcggatta cgtccgcgac acggcgtcgg
     2161 gcgaacgcga cgccgtcgcc ctgttcgcgg tcacctgcgg cgtgggcgtg cgcgagctgt
     2221 cgcaacgctg gaaggacgcg ggcgagtacc tgcggtccca cgcgctgcaa gccatcgcgg
     2281 tcgaggccgc ggaaggcatg gccgagtggc tccaccgccg gttgcgcacc gcgtggggat
     2341 tccccgaccc gcccacgacc acgatccacg acgtgctcaa gaacaaatac cgcggcatcc
     2401 gggtgagttt cggctacccc gcctgcccga acctcgcgga ccaacgcaag ctcttcgcgc
     2461 tcattcgtcc ccaggacatc ggagtggaat tgaccgaggg cgacatgatg gaccccgagg
     2521 cgtcggtgtc ggcattggtc tttcaccatc ccgaggccaa gtactttcgg gctgattgac
     2581 tcttgtcttc ggacggacgt aagatggctc gcaggaacgt tcgaggtacc atcaagggat
     2641 gaaccgcgtg cctgttcgtc gtcacaaatt tccggtgatg ctctggtcga ccggcgcgtg
     2701 gcgttccctc ttttccgtct gcctgctgat cgcctatgcg gtgctcgtcg gcgacgcgat
     2761 ccactgccag tattcctcgg ttgatcacga ccaccatgac gtcccgaagc cacagagcgc
     2821 ggagaacacg acccattgcc tggtcgccaa tcatgggtcc gccgcgcttc caaccatcgc
     2881 gccggggagc ctccccgggc tgcactcgga gcaataccgt ctcggatcag ttgcccttcc
     2941 gctcctgtcc tccgccttcg ttgtgacagc gagccgcgcg ccgccccgcc tgtagttcct
     3001 taccttctct tcacactgtt cgacacggtc gcagaccgcg cgaccacctt cgaccacctt
     3061 tgatgggtgt catcgtggcg ggtcgttgac gttcacttgg tccagaagga gggatctatg
     3121 ccgcgttatg gtttagggct gttgtacgtc gcactgacgc tgttcgtcgt atccgtccac
     3181 ggctcggcgt ggggacacgg ggtcgtgggt cagcggttct tcccggagtc gttgagcgtg
     3241 gaagatccgt tcccgtccga cgaagcggac tttccggcca tatccttcat caaaggtccc
     3301 gaaggcaaag aacgcgcggt cggactggag tactccaaac gactctcccc gaatctggcc
     3361 tttggattcg aggtcgaaca cgtctccctt tccccggctg acccgaccga accgcggcag
     3421 agcggcttga acaacccgga attgttcctc aagtacgcga tgcttcgcga tccggagcat
     3481 gaagcgatcg tctcggtgat cctgtccgtc gccccgccgt ggggggacaa agacttcacc
     3541 gagcgaaaca cgaccgttgc gcccggtctg atcttcggca aaggcttggg cggtcttccc
     3601 gacgggctgc gctacctcaa acccatcgcc ttgggcggcg ccgtgtcgtt ggagaccccg
     3661 ctcgggaata ttaccgaccc ggttgaccag accagcaccc tgaactacgg gttcatcgtg
     3721 gaatacagcc tcccctatct tcagtccttc gtgaaggaca tcgggctgtc gaagccgttc
     3781 agccgcatga tcccgatcgt ggagttcagc atggccacgc cggtcaacgg ccctgagcac
     3841 ggggcgacca cggcctacgt caacccgggc ttgatttggg ccggcaagta cgtgcaattc
     3901 ggcgccgaag cgctcatccc gttgaacgat cgcaccggcc ataatgtagg cgtgctggcg
     3961 gtcctccact ggtacttgga cgatctggcg ccgaacgcgt tcacttggac gccgtttcac
     4021 ggaacgctgg gccccacgag gaaatgacgg gccgcggatt cacacgtcga ctgatggcgg
     4081 cgggaggaat cctgaccggg ctcctcctgt tctcgtcgtc aatggcctgg gctcacgcgt
     4141 ttcccgacca ctccgagccg cgcgtggggt cggacctcaa ggcgccgcct tcgtcggtga
     4201 agatctggtt tgacagtgaa ctggagccgc tcttcagcac gatcgaagtg ttcgacgcgc
     4261 agcagcacaa ggtcgataac ggtgacggcg gggtcaacgc gaaggaccac tcgctcctgg
     4321 aagtgacggt gtcgtcgctc gcgcccggag cgtacaccgt gtcgtggcgg gtggtttccc
     4381 acgacgggca tcccaccgag ggccgattcc cctttaccgt gtcgggagcg cggtgagtct
     4441 gctcgacccg catatgctgc tgcacttggt ggtccgcgcc ttggaccttg ccgccatggt
     4501 catcgtggtc ggcgggttgg cgttcgatgc gttcgtcatg acgccggttc tcgggcgtct
     4561 ggatctctcc ccctccgagc gggcaccggt cgacgccgcg taccgcgcgc ggtatacgcg
     4621 gctcgtggga tgggcgctgg ccgtgctcgt gatcattcaa ttcgcggacc tgctcctgcg
     4681 cgtgcagatg atgagcggga aaccgttctc ggtcgtgatg ggcctgctgc cgtccgcgat
     4741 cacgggcaca cacatcggca aagtctggat cgctaagatc ggcgccctga cgatattggc
     4801 gatcgtgtgg ctggtcacgt atcggcggag cgcggcgccg tcaggcggat cgtggaaccg
     4861 gccgggcctc ctcgccatcg cggccatcgt cagcatcatg gtggcgctcg cgggccacgc
     4921 cgcggatcag ggcaacgtgt cgccggacgt tgcggccgat tggctgcacg tgctgtcgat
     4981 ctgtacgtgg gtgggcgggc tcgtcgcgtt catggtcctg gttccgacgg cgtcgcgggt
     5041 cgcgggtgag cggcacgcgg cccgtctgtt tacccgcgcg atggcgcgct tctcaaccgt
     5101 ggcgggttgg tcgctcggcg cgttgctcct caccggcgcg tacggggtct ggctgcacat
     5161 ccactcgtgg gccggcttgg ccggcgcgta cggcacggcc ctgctcgtca agctcgcgtt
     5221 cgtcgcgccg atgatcggcc tcggcgcgct ggggcggttc gacaatctcc cggagctgca
     5281 gaccatgacc ggcgaggcgt ttcggagcac gtggctcgct cgcgtcgccg ccgcgtcgtt
     5341 cgcgacgatc aggcgactgt ccggagtcga cccgcttcgg gccactcacg aaacgatcgc
     5401 ggcggttcgg tcccggtcgc tgcggtttat cgtatgggaa tgcgtgttcg cggtcggagt
     5461 gctcgcggga acctcggtgt tgacccagac catgccgccg cacgtgacgg acttcgccgt
     5521 tccggaggcg tctccgcacc acatgatgga catgccgaac ggatcgatgc cgtgagtggg
     5581 aataatcgtt catggccggc agttaatcgg ttcatgaagc tccgcctgcc gcaaccagca
     5641 cgtttcacgt ggcggatcgc ggtatccgcc gtcctgatgg tcgggtatct cgccttgatg
     5701 ggcgagagca tccactgtca gtacgtggcc gatgcccagc cggagcacca ccactcgtcc
     5761 tcatccgctc cgcccgtcaa ccaccagacg cactgtctgg ccgcgaacca cggcgcgacg
     5821 gccacgatcc actcagttgt cttgccgccg atcgatgtcc cgaaccaggt cggcttcctg
     5881 ctcaccaaga atcaaacggt tcccgactcc cgattcatcg cgccgggaac cgcccgcgcc
     5941 ccgcccgcgg tttagagcac ttccacaacc cagcgtctag cccgcgcgca caacggtatt
     6001 gtgacgcgcg cgggacgtcg tgcaccgcac gttctctcta tttcaaggag ctatcatgcg
     6061 cagagacgcc gcgctaattg caagtattgc gttcatcgcc gtcggatgga cggccgagct
     6121 tcccacggcg aacgccacat gcggatccgc caactgtttc ctggtgaccg ggacgcagga
     6181 aggcatcgcc aatccgggcc aggtcatcgc ggacctgtcg tatcgattca tccccatgga
     6241 ccaagtgcaa cggggatcca gcggcgcccc ggacgcggtc gtgccgaaaa tcgacttcac
     6301 caacaaccaa attatccaac cgggcgacat cgacagtcat cacgaaatcc ggaccaacaa
     6361 cgagctgatg caactcgatc tgacgtttgg gatcacgccc aagttcgcgc tgaccctcgc
     6421 agtccccttt tttaaccaac ggacgcacga acatgagcac gtcacgggcg gcacgccgtc
     6481 gaatccgaca tttggaagct tcagccgcga ggacggcacg gccgggttcg gcgatgttcg
     6541 tgtgatcggg aagtacgcgt tgctcatcca caccaagcac ctgctggtgg gaggcttggg
     6601 gatcaaggcg cccaccggcg agtacaaact ccgagaccat gagggagaga tcaacgagcc
     6661 gacgatccaa cccggcaccg gatcatggga cgggaccgcg tcgctgtact acgcgtatga
     6721 gatcttccct cacgaactca acacgttcgc gtcgacgtca taccaggcca acacggacaa
     6781 cgacctgcac taccaaatgg gcaacacgct cctgatcaac gccggggtga gttggttggc
     6841 gtcggaaaag gtgaacctga gcgcgcagat caacatgcgt caggcgccgc acgactcgtt
     6901 caacgacgaa acggtgccga gcacgggcgg ccgctgggtc tacttcacgc ccggcgttcg
     6961 tttccaggga gccccggaca cggcactgta cgcgcacgtc cagatcccga tttaccagta
     7021 cgtgaatgag gtcaacatcg tgccgcgcta tggattcatc atggggatat cacgcgcatt
     7081 ctgagacaca cacctcgggc acgacgacac gacagacaaa gaggattcga ctatgaagac
     7141 acgagtatcc actccggcgc gcctcggcgc gtatctgttg gccacggtca ccgtggtcgg
     7201 ttgtgggcag gcagccgttg ccccctcggc aaacaatgcc gcgtcaaccg gactcgaagc
     7261 gttggttttc gtcggcaaca atggcaacgg cacggtttca gtcatttccc acgggcacga
     7321 cggcaatgcc gtgctcaaca cgatcccggt cggatcgggc gcggtgggag acatcaacat
     7381 cagcagcgaa cgccacgtgt tcgtgaacgt cacggcgaac aaccaagttg cggcgatcga
     7441 cccggtggtc accggagacc cggtgctcaa aaatttcctg ccggtgggcc agaagccggt
     7501 ccatgggtat cgcgatccgt cggacggcac gctgatttgg gttctcaatg acgccaacgc
     7561 gacgagcgga acctgcgcca cggcccgaag cggggcagcg accaactccg tcaccgtcat
     7621 ccaaaaccac gaagtcggcg gcggaggggg cggcggcagc gggggcggac aatccacgtt
     7681 gggtatcgtg ctgaaagaga tttgcgtggg gcgtggacac cacaaggccg cgttctccta
     7741 cccgtcgcca actcaccagg gagtgccgcg tcgagcgttc gtcagcaaca tcagcgatgg
     7801 cacagtgacc gtgatcgact cggatccggc ctcggcgacc ttcggtgacg tggtcggcac
     7861 ggtcgatctc tgcgattcgg ccaagcagac ctgcgacacc gatccgacca cggggaatgg
     7921 cgcggtgccc cacggtatcg acttctcgcc ggtcagcggc ctggtctaca acgccaacgt
     7981 ggggtacggg acggtctggg ccatcgatcc aatcactccc gcgttggttg acgtcaatcc
     8041 agcgactccg gaagtcgatc cgatcaacgt tgggtacgcc aacaagtcgc atgtcagccc
     8101 ggacgggcgg tttctggtcg tgaagggcac ggacaccgcc tcgaacgccg atcatgtgat
     8161 cggcaaactc agtgtcgtgg atgtgtctga ccactccgtg caccagatcg acctgatgga
     8221 cattcacccc gacagctttg aattctcgcc ggacggaacc aagttgtacg tcaccaccgc
     8281 gacatcggtc gcctccggca acaccgtgca gccgaccacc atcaagaaca acgtgttgct
     8341 gtccttcaat gcgtctgccc tgccgcaact gccatcgccc atcgagattc cggttggcgt
     8401 tgcgggacac gaccaccgct cgctcgccat tcacgagcac aacggcgcgg ccgagcatgt
     8461 gtgggtgccg aacccggacg acgggaccgt gtccgtggtg gacgctgagt ccgacaccgt
     8521 ggtcgatacc gtgaccgtcg gcggcgagcc gtcgtccatc gccgtcttcg cgatcgagtg
     8581 acggccggcc gagagggtga ccgatgcaca cacgacatga ccactcagca ggtggggcgg
     8641 gggccaaggc tcccgcccgc tttctcctcg caaccctgct gttcgtcgcc ggggcgggcg
     8701 ccgcagcaac acaggctcac gcggcacctc cgcagatcgg caaacccgcc gcggactttt
     8761 cactgctcga cacgaccgag caaaacgtgc gcctttccgc gcttcgcgga cgccctgtcc
     8821 tcctcacgtt ctgggccacg tggtgcgaac cgtgcaaaat cgaaatgccg gaaattcaga
     8881 aggcctacga gcggttcaag accgacggct tcgtcgtact ggccgtgaac ttcggcgaga
     8941 aagccgagaa agcgagagcc tatgccgaga ccaacggctt gacgttcccc gtgttggtcg
     9001 atcgtcgagc caacgtggcc tcgcagtaca gcgtggtcag tctccccgtc agtcttttca
     9061 tcgacgccga cggcgtggtc caggaacgcg tcttcggcgg gacgctcacg ctggaacgca
     9121 ttgcgcaggt ccttcaccgg ctccgccaaa cgtcggatca gtagccggtc ttccggcgaa
     9181 cccctcctcc gcggccgcgt gtcgatccag acgggaagag aattacagag atcacatgtc
     9241 ccgccccata taacctcact ttgaacggaa aggcggggcc ggtattgctc gcggatgtcc
     9301 gatctctgct tcagtggcca gggaatgaaa ccgccctccc cgtggtcgct gtctatagcg
     9361 accgacagga acaggttttc tccttggaaa acaggacggt ctgcattcac ggtgccctat
     9421 cgacacacgt tcatgcggcg gagccttgaa cccgtgatgg cccgctaccg gaccctggcg
     9481 cgaagcgccg cggcggccct cctgttgctg agcggctgcg gcggaggggg cgactccggc
     9541 gggggcggcg ggggaacccc atcggacacg tctccgccca tattcagcgg cgtgacggtc
     9601 ggcgtcggca cgttgacgtc ggatcaggcc acggtcacct ggaacacgga tgagccttcg
     9661 acctcgcagg tgctctacgg gatcacccgc gcctacgcca gtagttccac gctggacagc
     9721 accttggtga ccagtcatag cgtgacgctg tacaacctca cgccgtccac gacctactac
     9781 ctgaaggcca agagcatcga cagcgcggga aatccgaaca catcgataga agtaattttc
     9841 accaccccca gttcctattt catcgtcacg accgacacgg
//
LOCUS       DBNGTT010000510         1608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1783419, whole genome shotgun sequence.
ACCESSION   DBNGTT010000510 DBNGTT010000000
VERSION     DBNGTT010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1783419"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1608
                     /locus_tag="AC16BC_11795"
     CDS             <1..>1608
                     /locus_tag="AC16BC_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3242342.1"
                     /translation="SGEIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSG
                     YLTRRLVDISQDVIVSEPDCQSTDGIFVTALVEGGEIIEPLDERILGRVASEPITDPL
                     TGDVIVGASEEIDEARVKATVEAGVDRVKIRSVLTCQSRRGVCAKCYGRDLARGKMVE
                     RGEAVGVIAAQSIGEPGTQLTMRTFHIGGTASKVIEQTVLEAKNTGVLKYLNLNTVKD
                     KHGDMVVMNRNGKIAVYDDTGREKEKYSVVYGAKIKVKDGGRVEVNQKLVEWDPYSFP
                     VLTEVGGKVAFGDILEGVTVHEEVDEVTGLSRRVVIDSPNSALRPRISIKDESGKTSK
                     LAANAVARYPLSAGAHILVEKNQTVSPGDVLAKIPRETTKTKDITGGLPRVAELFEAR
                     KPKEQAVISEIDGVVEYAGYVKGQRKVLVKNEMGDVREYLIPKGKHVNVHEGDWVHAG
                     EPLMDGSANPHDILDILGPKELQKYLVDEVQEVYRLQGVSINDKHIEVIVRQMLRKVK
                     VEDPGDTDFLIGSQVDKMAFAEENERA"
BASE COUNT          337 a          450 c          562 g          259 t
ORIGIN      
        1 cgtccggcga aatcatcgag acgccgatta ccgccaattt ccgcgaaggc ctgaccgtgc
       61 tccagtactt catctcgacc cacggcgcgc gcaaggggtt ggccgacacc gcgctcaaga
      121 ccgcgaactc cgggtacctg acccgtcgtc tggtggatat ctcgcaggac gtcatcgtca
      181 gcgagccgga ttgtcagtcc acggacggga tcttcgtcac cgcgctggtt gaaggcgggg
      241 agatcatcga gccgctcgat gaacgtattt tgggccgcgt ggcgtcggag ccgatcaccg
      301 acccgctcac gggcgacgtg atcgtgggcg cgagcgagga gatcgacgag gccagggtca
      361 aggccacggt ggaagcgggt gtggatcggg tcaagatccg gtcggtgctg acctgtcaat
      421 cgcggcgcgg cgtctgcgcc aagtgttacg gccgcgatct cgcgcgcggg aagatggtcg
      481 agcgcgggga ggcggtgggg gtgatcgcgg cgcagtccat cggcgaaccc ggcacccagc
      541 tcaccatgcg gacgttccac atcggcggca ccgcgagcaa ggtgatcgag cagaccgtgt
      601 tggaggcgaa gaacacgggc gtgctcaaat atctcaatct gaacacggtc aaggacaaac
      661 acggcgacat ggtggtgatg aaccgcaacg gcaagatcgc ggtgtacgac gacaccggcc
      721 gcgagaaaga gaagtactcg gtcgtctacg gcgccaagat caaggtcaag gacggcggtc
      781 gggtcgaggt caatcagaag ttggtggagt gggaccccta ttccttcccg gtgctgacag
      841 aggtcggcgg caaggtggcg ttcggcgaca tcctcgaggg tgtgaccgtg cacgaggaag
      901 tggacgaggt cacggggttg tcgcggcgcg tggtcatcga ctcgccgaac tcggcgctgc
      961 gtccgcgtat ttcgatcaag gacgaatcgg gcaagacgtc gaagctcgcg gccaatgccg
     1021 tggcccggta tccattgtcg gccggcgcgc acattctggt ggagaagaac caaaccgtgt
     1081 cgcccggcga cgtcttggcc aagatcccgc gcgaaaccac caagaccaag gacatcaccg
     1141 gcggtctccc gcgcgtggcg gaactgttcg aggcgcgcaa gcccaaggag caggccgtca
     1201 tcagcgagat cgacggcgtg gtggagtacg ccgggtacgt caagggccag cgcaaggtgc
     1261 tggtcaagaa cgagatgggc gacgtgcgcg agtatctgat tcccaagggc aaacacgtga
     1321 acgtgcacga aggggactgg gtgcacgcgg gcgaaccgtt gatggacggt tcggccaatc
     1381 cgcacgacat tttggacatt ctcggtccca aggagctgca gaagtatttg gtggacgagg
     1441 tgcaggaggt gtaccgcctg cagggcgtgt cgatcaacga caaacacatc gaggttatcg
     1501 ttcgccagat gctgcgcaaa gtgaaagtcg aggatccggg cgacaccgat tttctcatcg
     1561 ggagccaggt cgacaagatg gcgttcgccg aagagaacga gcgtgcgt
//
LOCUS       DBNGTT010000511         3360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2039189, whole genome shotgun sequence.
ACCESSION   DBNGTT010000511 DBNGTT010000000
VERSION     DBNGTT010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2039189"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="AC16BC_11800"
     CDS             complement(<1..1269)
                     /locus_tag="AC16BC_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3242343.1"
                     /translation="MAAIILTVSVGYLTSLYVTVTSRFEGPRWKLPSTVYAEPVTMYP
                     GMDLAAGHLRDRLLRLGYGPVATLPARPGEFRWTDTRLEIYLQDFSYPDHQVEGFPVA
                     ADLRDSRIVTLSRLGDGAPMTSVDLEPEVIAGLFDDSWEGRRLVRVGEVPRSLIDAIL
                     AAEDARFYQHWGIDVRAVARALWTDLKQGQWVQGGSTLTQQLVKNFYLTQERTLYRKV
                     VEAIMAVMLEARYQKPEILEAYLNEIYLGQRGDMGVFGVGEASILYFGKEVERLTTAE
                     SALLAGMIRSPRANNPQTAPARAQATRDYVLRRMATLQMIGADEAETAMLEPIPSRPK
                     ARAAVRAPYFADLVRDELERLYSRDALASEGLRIFTSLDVEAQRAVEEALRAGLAALD
                     RRAPKASETLQGGAIVLQPQTGYVRALVGGR"
     gene            1504..2676
                     /gene="hemW"
                     /locus_tag="AC16BC_11805"
     CDS             1504..2676
                     /gene="hemW"
                     /locus_tag="AC16BC_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR3242344.1"
                     /translation="MSHSEGISGREQIFPPGFGLYIHVPFCISKCAYCAFSSIAGGAR
                     LMEPYIEAVRREIDAHADMASGRMVDSLYLGGGTPSLLPPAAIAALLHHCRSRFRFAP
                     GAEVTLEANPETVTPDRVGAWLDAGVARVSMGVQSLDEQELATLDRAHTARRAESAFK
                     MIRAAGALNISVDLLYGLPGQTVERWRETVHRALDLDAEHLSAYALTPEPGTPIGSAV
                     AEGTMALPSDDLVLAQEAVLQEAVVQSGLERYEISNYAKPGFECRHNVLYWRCDDWLG
                     FGASAHSHIQGQRWWNRFAPDDYMRGVAGGDPVDGRESLSVDQRLGETLAFGLRMVRG
                     VPRQLFIDRFGQDAWTAKGPGLGHLVEDGLLAGTRDTIRLTRRGLAFADSAAALLI"
     gene            complement(2754..2966)
                     /locus_tag="AC16BC_11810"
     CDS             complement(2754..2966)
                     /locus_tag="AC16BC_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3242345.1"
                     /translation="MARGKVKWFNASKGYGFISQEDGSDVFVHFSAIQGDGFKTLDEG
                     QEVEFDVVKGAKGPQAANVVKTTVKA"
BASE COUNT          612 a         1095 c         1001 g          652 t
ORIGIN      
        1 cctgcccccg acaagggcgc ggacataccc ggtctggggc tgcagcacga tcgcgccgcc
       61 ttgcaacgtt tcggatgcct tgggcgctcg ccggtcgagc gccgcgagcc ccgcgcggag
      121 cgcttcttcc accgcgcgct gggcctccac gtctaaactg gtaaaaatcc gcaatccctc
      181 ggacgccagc gcatcgcgcg agtagagccg ttcgagttcg tcccgcacca gatcggcaaa
      241 atacggggct cggaccgccg cgcgcgcctt ggggcgggac gggatcggtt ccagcatggc
      301 ggtttccgct tcgtcggcgc cgatcatctg gagcgtcgcc atccggcgca gcacatagtc
      361 ccgcgtcgcc tgagcccggg ccggagccgt ctggggattg ttcgcccgcg gggagcggat
      421 catcccggcc agcagcgccg attccgcggt cgtcagccgc tcaacctcct tgccgaagta
      481 gaggatcgac gcctcgccca cgccgaagac gcccatgtcg ccgcgctgtc cgagatagat
      541 ttcgttgaga tacgcctcca ggatttccgg tttctggtat cgcgcctcca gcatcaccgc
      601 catgatggct tcaacgacct tgcggtagag cgtgcgctcc tgcgtcagat agaaattctt
      661 gaccaactgt tgcgtgaggg tgctgccccc ttggacccac tgaccttgtt tcaaatcggt
      721 ccagagcgct ctggccacgg cccgcacgtc gatgccccaa tgctgataaa aacgcgcgtc
      781 ctcggccgcc agaatcgcgt cgatcagcga gcggggcacc tcgccgactc gcaccagccg
      841 gcggccctcc caactgtcgt cgaagagccc ggcgatgacc tcgggttcca ggtccacgct
      901 ggtcatcggc gcgccgtctc ccagtcgact caacgtgacg atgcgcgaat cgcgcaggtc
      961 cgcggcgacc gggaatccct ccacctggtg atccggatat gagaagtcct ggaggtagat
     1021 ttccaagcgg gtatcggtcc accggaactc cccgggccga gcgggcagcg tggccaccgg
     1081 accgtaaccg agacggagca ggcgatcgcg aaggtgcccg gcggccaggt ccattccagg
     1141 atacatggtc accggctcgg catacaccgt ggaggggagc ttccaccgcg gaccttcaaa
     1201 ccgcgacgtc accgtcacat acaatgatgt cagatagccg acgctcaccg tcaagatgat
     1261 cgcggccaac cacggaaatc ccctccgtcg gcgtctccga cgggacggac ccactcgcct
     1321 catccagcga tcatctcccc agccggtcgt tttccctgcc acaacaattg tcctcggtca
     1381 ccacgccgtc tacgtctgca caccgatgcc aacgtggggc ggcatcgtga ccaacgtggc
     1441 gccccacctt gacaccccgt accgtgggtg ggatcatacc acgtccaatc cctgaacgag
     1501 accgtgagcc attcggaagg catctccggg cgcgaacaga tcttcccgcc cggtttcggc
     1561 ttgtatattc acgtgccgtt ctgcatcagc aaatgcgcgt actgcgcatt cagctcgatc
     1621 gcagggggcg cccgattgat ggagccgtac atcgaagccg tgcgtcgcga gatcgacgcg
     1681 catgccgata tggcttccgg ccggatggtc gattccctct atctgggggg cggaacgcct
     1741 tcgctgctcc cgccggccgc gatcgccgcg ttgttgcatc attgccggag tcggttcagg
     1801 ttcgcgccgg gcgcggaagt gacgcttgag gccaatccgg aaaccgtcac gccggatcgt
     1861 gtgggcgcgt ggctggatgc cggagtcgcg agggtcagca tgggcgttca atcgctcgat
     1921 gagcaggagc tcgccacatt ggatcgcgcg cacaccgcgc gccgggccga gtcggccttc
     1981 aagatgattc gcgcggccgg cgctcttaat atcagcgtgg atttgctgta tggccttcct
     2041 ggccagaccg tcgagcgttg gcgcgagacc gtccatcgcg cgctggacct cgacgctgag
     2101 cacctttcgg cctacgcgct cactccggag cccggcacgc caattgggtc ggcggttgcc
     2161 gagggaacca tggcccttcc gtcggacgac ttggtcctgg cccaagaggc agtcctgcaa
     2221 gaagccgtgg tgcagtccgg cctcgaacga tacgagatct ccaactacgc gaagccaggg
     2281 tttgaatgtc gacacaatgt attgtattgg cggtgtgatg actggctggg tttcggggcg
     2341 agcgcgcact cccacatcca gggccaacgc tggtggaacc ggttcgcgcc ggacgactac
     2401 atgcggggcg tagccggcgg agacccggtt gacggacggg agtcgctcag cgttgatcag
     2461 cgactgggag agacgctcgc cttcgggctg cgaatggtcc ggggggtgcc ccgccaactc
     2521 ttcattgacc gcttcggaca ggatgcttgg accgcgaaag ggcccggcct cggacacctc
     2581 gtggaggacg gattgctcgc gggcactcgg gacactatcc ggctcacgcg tcgcgggctg
     2641 gcgttcgccg actccgcggc cgccctcctg atttagcacc ctgaaacgcg cgcgcccttc
     2701 ggaatccaat tccgaagggc gccgggattc ttcacactgg agggtcggat gcgctaggct
     2761 ttcaccgtgg tcttgacgac attggcggct tgcgggcctt tggccccttt gacaacgtcg
     2821 aattccactt cctggccttc atccaaggtc ttgaatccgt caccctgaat cgccgagaag
     2881 tgaacgaaga cgtcactacc gtcctcctgg gagatgaacc cgtacccctt gcttgcgttg
     2941 aaccacttta cctttcctct tgccaccgcg cacgccctcc tttcggacca gaatccagac
     3001 gtcgacccgt ccccttatca ataataagga gacaactttc caatcccgca ttgtcgctta
     3061 tagcaaatga cccatatcgt gtcaagcaat tttgacattt tatggaattt ttcgtctcat
     3121 cgactggtta tagattttcc attgtcaggt cgcgaacatg gcatggaggt ttttttccct
     3181 aaaaccccgt aaataaaagg tacggcgcga tgcgaggaaa aattccaggg aaaaactccc
     3241 gataaattac cctcaaaccg tgtcaacctg gatgaagtgg tggcctaaag acgaggtagc
     3301 gtgatgcctt gctgtgcctg gtattttccc ttgcggtccg cgtatgacgt ttcacaactc
//
LOCUS       DBNGTT010000512         3121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1783805, whole genome shotgun sequence.
ACCESSION   DBNGTT010000512 DBNGTT010000000
VERSION     DBNGTT010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1783805"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..627
                     /locus_tag="AC16BC_11815"
     CDS             115..627
                     /locus_tag="AC16BC_11815"
                     /inference="COORDINATES: protein motif:HMM:NF017913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HIR3242346.1"
                     /translation="MDEKPKESERSSFSRQLIFGRPVSAAAIGVMETLDGWGYVTAGI
                     SFLALGMVVFVHAWYVFAVTVGSKVLPAVLGLVHDLLLVIILLELFRTIINFLKTKII
                     TLEPFLYICIIASTRRILTTGAQIANLEDLSDQLFQRYLLDVGANVLVIVALVIALYV
                     VRKAPQRSPT"
     gene            1038..1772
                     /locus_tag="AC16BC_11820"
     CDS             1038..1772
                     /locus_tag="AC16BC_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3242347.1"
                     /translation="MTCGPLYASESDDRIESSARNSHMFKTYLTGDDIRVQARDGVVT
                     LRGTVTHEFHLALAQDTVAGLPGVTRVDNRLGLSTGRMVAYSDAWLSTRVKTTLLFRR
                     TVRARHIEVTAIDGMVILRGEVSSQAQRDRLTEYIDDVIGVEGIRNKMTVARTASTTT
                     AGDAAGETIDDASVTAQVKTALMFHRSAGSRTIQVKTTKGVVTLRGTARNAAHQALVA
                     KLVTDIDGVKSVVNDITVDTALSRND"
     gene            2384..2527
                     /locus_tag="AC16BC_11825"
     CDS             2384..2527
                     /locus_tag="AC16BC_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242348.1"
                     /translation="MTLDEQIAKMTKAINQAIKVMSDRFGSTEGDVARAVQDLKASLQ
                     TSA"
     gene            2617..>3121
                     /locus_tag="AC16BC_11830"
     CDS             2617..>3121
                     /locus_tag="AC16BC_11830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3242349.1"
                     /translation="MITPPRNGTLRWPLPEQDHWSRPFAEVLLRHLDLYPGATVLDVA
                     CGRGIPAFYLAEQVGPAGEVLGIDVNEHKIACARELQRQHLPWLRFECRDMRFLSSRV
                     PKVDRITGNLAVLFFRPNRFETLRGLVDHLKPGGQLVLTFPSRGTFDLLWRRIDEELA
                     ARGLGAER"
BASE COUNT          587 a          874 c         1014 g          646 t
ORIGIN      
        1 agagttcagg ccaggtgatt ggaggccctg agacagtttc tgaactacca ggagcgtccc
       61 gttggttcct cgctgattgc tttaatcata ttgaagtgtt agggtccgcc gcctatggac
      121 gagaaaccca aagagtcgga gcgaagttcg tttagccgcc agttgatctt cggtcgtccc
      181 gtcagcgccg cggcgatcgg ggtgatggag accttggacg ggtggggcta tgtcacggcg
      241 gggatcagtt tcctggcgct gggcatggtg gtgttcgtcc atgcctggta tgtcttcgcg
      301 gtgacggtgg gttcgaaggt tttgcctgcc gtgttggggt tggtccatga tctcttgttg
      361 gtgatcatcc tcttagagct ctttcgaacc atcattaatt tcctaaaaac caaaatcatt
      421 acgttggagc cgtttctgta tatctgcatt atcgcgtcca cgcggcgtat cctcacgacc
      481 ggcgcccaga ttgccaacct cgaagacctg agcgatcaac tcttccaacg gtatctgctg
      541 gatgtggggg ccaatgtcct ggtgatcgtc gcgttggtga tcgcgttgta cgtggtccgc
      601 aaagctccgc aacgcagtcc gacctgatgc cggcgatgcc gcataaagcc tgggaaagtt
      661 cgtgttatcc catcccgggg tcttgagagg cgctgaattc gtgaacgatt tcagccccgc
      721 ctccaatttc aaacaatcgc gcttgaccgg gcctgctgat cagcgtatta ttgttttgtg
      781 acgttctagc ctcgctggct gccctatcct tcgcgcttgt cgtgttcctc gcgtgaccga
      841 ttctccagtc tccgccgctt ctacgccgaa gtcgttgtag ctgttgcctg cagcgagcgg
      901 caaccgataa cggaaccgac ggtggccttc tttcgtaatc gtttgaggtt catcgtctcc
      961 gaggagaggc gcgaacagtg aaacaatcgg atccacgata tccaggggca ctcttggcgg
     1021 ccgcggttgt cctgtgcatg acctgcggac ctctgtacgc ctccgagtcc gatgaccgca
     1081 tcgaatcctc ggcgcggaac tcgcatatgt tcaagaccta tctcacgggt gacgacatcc
     1141 gtgtccaggc ccgggacggc gtcgtgacgt tgcgggggac ggtgactcac gaatttcatt
     1201 tggccttggc ccaggatacg gtggccgggt tgccgggcgt cacgcgcgtg gacaatcgcc
     1261 tcggcctcag caccggccgc atggtcgcct actcggatgc atggctcagc acgagggtga
     1321 aaaccacgct cttattccgg cggaccgtgc gggcccggca catcgaggtg accgccatcg
     1381 atgggatggt gatcctgcgg ggggaggttt ccagtcaggc tcaacgcgac cggctcaccg
     1441 aatacatcga cgacgtcatc ggcgttgagg gcatccgcaa taagatgacc gtggcgagga
     1501 cggcaagcac gaccacggcg ggtgacgcgg ccggtgaaac aatcgatgac gcctcggtca
     1561 cggcccaggt caagaccgcg ctgatgtttc atcgctcggc cggcagccgc acgatccagg
     1621 tcaagaccac gaagggcgtc gtcacgttgc ggggaacggc ccgcaacgcc gcccatcaag
     1681 cccttgtcgc caagctggtc acggatatcg acggggtgaa aagcgtcgtg aatgacatta
     1741 cggttgacac cgccctctcc aggaacgact aggcgggaca ttgtggccgg cgggctcatc
     1801 catatccagg tgatggtggc cgtgatcatg gtctcggtga gcgtcatccg ggggcggaaa
     1861 cgcccctgac caacggcagg ttcaatggac aagcgggacg tgggcgacac gaacaccaca
     1921 cagcatggga ggcgttatgg actttaacaa aggcgttgtg acgcgggaag cctggggctc
     1981 caagccgtgc aagcatccca ccaaggagtc ggagtttttt atggggaaga atacggggaa
     2041 gaccgtgtgt acgatctgcg gacagctgtt tctcggcgga gtacgggtcg cctcctgacg
     2101 cgtgagcgcc cgatcaagcc atcgatcgcg gcgcgagttc ctcgtatcag gattagggag
     2161 aacacgatcc tcgtgaagag ggagagaggt ggatggaatg tatcttgcgt ggagcggtat
     2221 cgcggcgttg gtcggcgtgt tgtgtctgac gttggggctg gctgtaaggg gatcggtgag
     2281 caaaaagagg ggctaaacaa gcttgcttcg catcggtcat cagagcgggc ccgcattcgt
     2341 ccgcagtggg tgacgattga gcggcgagct taggaggaac accgtgacac tggacgagca
     2401 aattgcgaag atgacgaaag ccatcaatca ggccatcaag gtgatgagcg accggttcgg
     2461 ctcgaccgaa ggcgacgtgg caagggccgt gcaggatctc aaggcgtcgc tccagacctc
     2521 agcgtgatga cggatcaggt cggtccacag cgaggggccg cgcccgcgga gcaggagggc
     2581 agcgggtcac ctcagacacc tcccggtgtc ggcggcatga tcacgccgcc ccgcaacggg
     2641 acgttacgct ggccgctccc cgaacaggat cattggtcca ggccgtttgc cgaggtgttg
     2701 ctgcgccacc tggatctcta cccgggagcg acggtgctgg atgtggcgtg cggccgcggt
     2761 atccccgcgt tctatctggc ggagcaggtc ggtccggcgg gggaggtgct gggtatcgac
     2821 gtgaacgagc acaaaatcgc ctgtgctcgg gaactgcaac gccaacatct tccgtggctg
     2881 cgtttcgagt gccgggacat gcgttttctg tcctcgcgcg tgccgaaggt tgaccgcatc
     2941 accggcaacc tcgcggtgtt gttctttcgt ccgaaccggt tcgaaacgct tcgcggattg
     3001 gtcgaccatc tgaagccggg cggtcaactg gtcctgacct tcccgtcccg cggcacgttt
     3061 gatttgctct ggcggagaat cgacgaggaa ctggccgcgc gcggtcttgg cgcggagcgg
     3121 c
//
LOCUS       DBNGTT010000513         4855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2294680, whole genome shotgun sequence.
ACCESSION   DBNGTT010000513 DBNGTT010000000
VERSION     DBNGTT010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2294680"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="AC16BC_11835"
     CDS             complement(<1..1299)
                     /locus_tag="AC16BC_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3242350.1"
                     /translation="MYKTIYVPVDNSDHSNRAIEIAVKLAQQFGATVVGSHAYAAKMH
                     DKRFKQMEAGLPEEYHDEKELERQRRIHDSLITRGLEIITDCYLDVVDQQCKSANVGF
                     ERRFLEGKNFQVLVDDIKTNKYDLVVMGSLGVGAVRDSVIGSVVERTVRRVHGSDFLI
                     IKNPKTIEPDGPIVAALDGSPHSYGGLMTALALGKALNVPVEAISAFDPYFHYAMFNS
                     IADVLSEEAGKVFRFKEQEKLHEEIIDSGLAKIYQAHLDIGKQVAQAEGMELKTTLLD
                     GKAFERCLQYVKKNKPWLLIMGRIGVHSDDTMDIGSNSENLMRLAGCNILISNRTYVP
                     PIDTMAQYTIAWTEEAAKRMEKVPVFARGVARTAIYRYAIEKGHTIISNEVVDAAMGH
                     LLPPDAVAAMKGLGKTLDAAGVDRNKMEASDEVVKDLMGAQ"
     gene            complement(1446..2276)
                     /locus_tag="AC16BC_11840"
     CDS             complement(1446..2276)
                     /locus_tag="AC16BC_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3242351.1"
                     /translation="MTYELLSFGFMQRALVASVIIGALCAVIGVFVVLRGLSFIGAGT
                     SHAAFAGVTFGYLTGLSPLVMAIAFGLGTVWIVHGLYQRGVMKLDASIGIFYTFTMAL
                     AILFLGLMGTYNAEVYGFLFGSVLSVTREDIRVMLWLAGGVLALVVLFYKEFHFITFD
                     PVMAEATGIAAGRLFFLLLNLIALTVVVSLKTVGALLVFAMIVIPAAAAYQLARTMLT
                     MMAYAVAIGVGAAVGGVLLSYEFDLPSGATIVLVATLFFALAVVASPKRRRLFPARSG
                     "
     gene            complement(2313..3101)
                     /locus_tag="AC16BC_11845"
     CDS             complement(2313..3101)
                     /locus_tag="AC16BC_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR3242352.1"
                     /translation="MTAPDVITFDHATIDYRDRPALEDVTLGIAAGEFIGVIGPNGSG
                     KTTFLKAILGLIHPTAGSVRVFDCACHRLRCEHLARIGYLPQHEHVDPQFPITVAEAV
                     MMGRYSTIGLFRYPRSADRAIVREALEAVDLLPVAGAAVGQLSGGQQQRVMIARALAQ
                     RPEILLLDEPTTGIDAPTQHAIMELITKLHRDMRLTILFVTHDINLISRVADRLVLLK
                     TRLFAAGPPNEVLRRDTLAQVYGQDIVVTNHGAAHYVIVGDHHA"
     gene            complement(3098..4024)
                     /locus_tag="AC16BC_11850"
     CDS             complement(3098..4024)
                     /locus_tag="AC16BC_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR3242353.1"
                     /translation="MSSTAVRALIAALLIGLISAGRSASAEPLPVVATLPVLADFVRE
                     VGGDAVTVTSLITGLESEHTYTPKPSDIEAVRRAAVLVKVGLGLEVWVNGLIANAENP
                     RLIVVDTSQGIGLMRGGDAHGHPDEHDTLPHAEERRSGGNPHVWLDPENAQAMVRHIT
                     DGLIRADPARRKVYLAGQAAYLKQLDDLTRTLTAQFAAVPDHKIITHHAAWPYFARRF
                     DLRIRGEIFTQIGAEPSAKRIAELIRTIRKEKIRVIVSEPQLSPKIPDALAQETGAKV
                     VILTPLPGALPGTEDYLSMMRYNASALLEALR"
     gene            complement(4021..4500)
                     /locus_tag="AC16BC_11855"
     CDS             complement(4021..4500)
                     /locus_tag="AC16BC_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242354.1"
                     /translation="MDDREHAHQPVAPESGPGPAPRANRRIWLWLVLVAIATAAVWTY
                     TKVGAPSLVGEWSNRTPQNQVTFTFERNGSGAMSIGNARLEFQYTLDRSHVPAWLDLR
                     GAVEGKPVTIRAIAELPRHDKLKIRMPHTRTPGERPTEFVADDVENTILLTRVESAS"
     gene            complement(4500..>4855)
                     /locus_tag="AC16BC_11860"
     CDS             complement(4500..>4855)
                     /locus_tag="AC16BC_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIR3242355.1"
                     /translation="DGDRALFQSVIDDLYGRFVDAVAAGRPKLARERIVELADGRIYT
                     AEQAKSAGLVDGLGYLDDSIATLRDAVGLTSATVVTYTRPGEYKGSVYAAPTVNIDLT
                     LWPTRHAGFLYLWQP"
BASE COUNT          848 a         1535 c         1596 g          876 t
ORIGIN      
        1 ctgcgcaccc atcaggtcct tcacgacctc gtcgctggct tccatcttgt tgcggtccac
       61 gcccgcggca tccagggtct tgccgagccc tttcatggcg gccaccgcgt ccggcggcag
      121 cagatggccc atggccgcgt ccacgacctc gttggaaatg atggtgtggc ccttctcgat
      181 cgcgtagcgg tagatcgcgg tgcgggccac gccgcgcgcg aacaccggca ccttctccat
      241 gcgcttggcc gcttcctcgg tccacgcaat ggtgtactgc gccatggtgt cgatcggggg
      301 cacgtaggtg cgattggaga tcaagatgtt gcagcccgcc agacgcatca ggttctcgga
      361 gttgctgccg atgtccatgg tgtcgtcgga gtgcacgccg atgcggccca tgatcaggag
      421 ccacggcttg ttcttcttca cgtactgcag acagcgctca aacgccttgc cgtcgagcag
      481 ggtggtcttg agttccatgc cctcggcctg ggccacttgt ttcccgatgt ccaggtgcgc
      541 ttggtagatc ttggcgagcc cggagtcgat gatttcctcg tgcagtttct cctgttcctt
      601 gaaccggaac accttgccgg cttcctcgga gagcacgtcc gcgatgctgt tgaacatcgc
      661 gtagtggaag tagggatcga acgcggagat cgcctccacc ggcacgttca acgccttgcc
      721 aagggcgagc gcggtcatca ggccgccgta agagtgcggg cttccgtcga gcgcggccac
      781 gatgggcccg tcgggttcga tcgtcttggg atttttgatg atcaaaaagt ccgacccgtg
      841 cacccggcgc accgtgcgtt ccaccacgct gccgatcacg ctgtcgcgca ccgcgcccac
      901 gcccagcgag cccatgacca ccaggtcgta cttgttggtc ttgatgtcgt ccaccaggac
      961 ctggaagttc ttgccttcca ggaagcggcg ttcaaagccc acgttggcgc tcttgcactg
     1021 ctgatccacc acgtccagat aacagtccgt gatgatctcc agaccgcggg tgatcagcga
     1081 atcgtggatg cggcgttgac gttcgagttc cttttcgtcg tggtattcct cgggcaggcc
     1141 cgcttccatc tgcttgaacc gtttgtcgtg catcttcgcc gcgtacgcgt ggctgccgac
     1201 cacggtggcg ccaaactgct gggcgagttt gaccgcgatt tcaatggcgc ggttggaatg
     1261 gtccgaattg tcgacgggaa cgtaaatcgt cttatacatc aaatctctcc tctacgaacg
     1321 cgtgcgggtg tcgcacgaac gaccgagtcg gtgaaccgaa ggggccacct cggaacggtc
     1381 agtagaaaat cggctcatta tatgccggta gtccaatgaa tgcaatgcta tcggtcggcg
     1441 ggcggttacc ccgaacgcgc ggggaacagc cgtcgccgtt tcggcgacgc gaccacggcg
     1501 agcgcgaaga acagggtggc gaccagcacg atcgtggcgc ccgagggtag gtcgaattca
     1561 taggacagga gcacgccgcc cacggccgcg cccacgccga tcgcgacggc ataggccatc
     1621 atggtcagca tggtgcgcgc cagttggtac gcggccgcgg ccggaatcac gatcatggcg
     1681 aagaccagca gcgcacccac ggttttgagc gaaaccacca cggtcagggc gatcaggttc
     1741 agcagcagga aaaacagccg tcccgctgcg atgccggtgg cttcggccat gaccgggtcg
     1801 aacgtgatga agtggaattc cttataaaac agcacgacca atgcgagcac gccgcctgcc
     1861 agccacaaca tcacccggat atcctcgcgg gtgaccgaga gcacgctgcc gaacaggaag
     1921 ccgtagacct ccgcgttata cgtgcccatc aagcccagga acaggatcgc cagggccatg
     1981 gtaaaggtgt agaagatgcc gatcgacgcg tcgagcttca tgaccccgcg ttgatacagc
     2041 ccgtgcacga tccacaccgt gcccaagccg aacgcgatcg ccatgaccag cggcgacaga
     2101 ccggtgagat agccgaacgt cacccccgcg aacgcggcgt gggacgtgcc cgcgccgata
     2161 aacgacagcc cgcgcaagac cacgaacacg ccgatgaccg cgcacaacgc gccgatgatc
     2221 accgacgcga ccagcgcgcg ctgcataaaa ccgaaagaga gcagttcata ggtcatatag
     2281 gacctatggg acctataaag gtcaaaggct tctcaagcat gatgatcccc cacgatcacg
     2341 tagtgcgcgg cgccgtgatt ggtgaccacg atgtcctggc cgtagacctg ggccaaggtg
     2401 tcccgacgca gcacctcgtt gggagggccg gccgcgaaca gccgggtctt gaggagaacc
     2461 agccggtcgg ccacgcggct gatcaggttg atgtcgtggg tgacaaagag gatcgtcaac
     2521 cgcatgtcgc gatgcagctt ggtgatcagt tccatgatcg cgtgctgcgt gggcgcgtcg
     2581 atgccggtgg tgggctcgtc gagcaacaga atctcgggcc gctgggcgag cgcgcgggcg
     2641 atcatcacgc gctgctgttg gccgccggac aactggccca ccgcggcgcc ggccacgggg
     2701 agcaggtcca cggcttcgag cgcctcccgc acaatggccc ggtccgcgct cctcggataa
     2761 cgaaacaacc cgatggtgct gtagcggccc atcatcacgg cttccgccac cgtgatggga
     2821 aactggggat ccacgtgctc gtgctgcggc aggtacccga tccgggcgag atgttcgcac
     2881 cgcaggcgat ggcacgcgca gtcgaacacg cggaccgacc cggcggtggg gtggatgagg
     2941 cccaggatcg ctttgagaaa cgtggttttg cccgatccgt tggggccgat cacgccgatg
     3001 aactctcccg cggcgatccc gagggtgacg tcctccagcg cgggacggtc gcggtagtcg
     3061 atggtcgcgt ggtcgaaggt tatgacgtcc ggcgccgtca ccgcagcgcc tccagcagcg
     3121 cggaggcgtt gtatcgcatc atggacagat agtcctccgt gcccgggagc gcgcccggca
     3181 gcggggtgag gatcacgacc ttggccccgg tttcctgcgc gagcgcgtcc gggattttcg
     3241 gcgagagctg gggttcggag acgatcacgc gaatcttttc cttccggatc gtccggatca
     3301 gttccgcgat gcgtttggcc gacggctccg cgccgatttg ggtgaagatt tcgccgcgga
     3361 tgcgcaggtc gaaccgccgc gcgaagtagg gccacgcggc gtggtgggtg atgatcttgt
     3421 ggtcgggcac cgccgcgaat tgtgcggtca aagtccgggt caggtcgtcg agttgtttga
     3481 ggtacgcggc ctgtccggcc aaatagacct tgcggcgagc agggtcggcc cggatcagcc
     3541 cgtcggtgat gtggcgcacc atggcctgcg cgttttccgg gtccagccag acgtgggggt
     3601 tgcccccgct gcggcgttcc tcggcgtggg ggagggtatc gtgctcgtcc gggtgcccgt
     3661 gggcgtcgcc gccccgcatg aggccgatgc cctgcgacgt atccacgacg atcaggcgcg
     3721 gattctcggc gtttgcgatc aagccgttga cccacacttc gagccccagc cccactttga
     3781 cgagcaccgc ggcccgacgc acggcctcga tgtcgctcgg tttcggagtg taggtgtgtt
     3841 cgctttcgag tccggtgatc aggctggtca cggtcacggc gtcgccgccc acttcgcgga
     3901 cgaaatcggc gaggaccggc agcgtggcca cgaccgggag gggttcggcg gacgcggagc
     3961 ggcccgcgct gatcagcccg atcaacagcg ccgcgatcag ggccctcacc gcggttgaac
     4021 tcatgaagcg gactcgacgc gggtcaggag gatggtgttt tcgacgtcgt ccgcgacgaa
     4081 ttcggtcggg cgttcacccg gcgtccgcgt gtgcggcatt cggatcttga gcttgtcgtg
     4141 gcggggcaac tcggcgatgg cgcggatcgt gacgggtttc ccttccacgg ccccgcgcaa
     4201 atccagccat gccggaacat ggcttcgatc cagggtgtac tgaaactcca ggcgcgcgtt
     4261 gccgatcgac atggcgcccg agccgtttcg ctcgaacgta aacgtcacct ggttttgcgg
     4321 cgtccggttc gaccattcgc ccacgagcga aggcgcgccg accttggtgt acgtccagac
     4381 ggccgcggtc gcgatcgcga caaggaccag ccatagccag atccggcgat tcgcccgcgg
     4441 ggcgggaccg gggccggatt ccggcgcaac gggttgatgg gcgtgctcgc ggtcgtccat
     4501 cacggttgcc agagatacaa aaatcccgcg tgccgggtcg gccagagtgt caggtcgatg
     4561 ttgaccgtgg gcgcggcata gacgctgccc ttgtattccc cgggtcgcgt gtacgtcacc
     4621 accgtcgcgg aggtcagccc gaccgcgtcc cgcagcgtgg cgatcgagtc gtcgaggtat
     4681 ccgaggccgt ccaccaggcc ggcggacttg gcctgctcgg ccgtgtagat ccgtccgtcg
     4741 gccaattcaa cgatccgttc gcgcgcgagt ttgggccggc ccgcggccac cgcgtccacg
     4801 aaccgtccgt acaggtcgtc gatcacggac tgaaacaagg cgcgatcgcc gtcct
//
LOCUS       DBNGTT010000514         6954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_762499, whole genome shotgun sequence.
ACCESSION   DBNGTT010000514 DBNGTT010000000
VERSION     DBNGTT010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_762499"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /gene="nagZ"
                     /locus_tag="AC16BC_11865"
     CDS             <1..947
                     /gene="nagZ"
                     /locus_tag="AC16BC_11865"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR3242356.1"
                     /translation="VAALSRSVVEAAAAATSTKAPPLVAIDQEGGRVSRLPPPFTQLP
                     PAAALGARKAVALTYAIGEIMGRELAAIGVNMNMAPVLDVNTNAANPIIGDRAFGDSP
                     MIVEEHGLALMVGLQDHGVIACAKHFPGHGATSADSHLELPVVDASLRQLERIHLRPF
                     AHAIANRLSALMTAHVRYPALDARSPATLSKKIVTGLLRRAMGFDGVVITDDLEMKAV
                     ADRHDAGSAALKAFQAGADVLLFCRDQDAPEQAIDALADAVKRKRLSEARVDQSLNRI
                     LRLKERFLLGAKPPSASRLRDIVGCEAHLRLVAQIKSS"
     gene            complement(1029..1253)
                     /locus_tag="AC16BC_11870"
     CDS             complement(1029..1253)
                     /locus_tag="AC16BC_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3242357.1"
                     /translation="MQKYSPTQVGQRIREIRGGMTQTEFAKAIGVKKQNYISRYERGR
                     VPNPELLLRIADFGKVSVDWLLTGKSSKRK"
     gene            complement(1337..2329)
                     /locus_tag="AC16BC_11875"
     CDS             complement(1337..2329)
                     /locus_tag="AC16BC_11875"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3242358.1"
                     /translation="MASSAGGAPQEASAASTLITVTAGEAAERIDRFLARQGLPLSRA
                     RIQRGIDEGLIEVNGRRTKASYLIRPGDRIVICHPPPQPVEVRAEPIPLHIVYEDEDL
                     LVLDKPAGLVVHPAPGHAGGTLVNALLHHCRDLAGIGGQARPGIVHRLDKDTSGLLVA
                     AKHDAAHTGLMRQFKAHSIARRYLAIVAGDVRPRKGTIDLAIGRDVWERKKISARTTS
                     PRHAVSHYEVVERYGRATLVAVTLETGRTHQIRVHFAHLGFPVLGDPVYGGRRAVPPP
                     DVPVHRQLLHAQQLGFIHPISGRPMVFTAPMPEDMQAARRVLTASAGHPGRPLS"
     gene            complement(2335..2868)
                     /gene="lspA"
                     /locus_tag="AC16BC_11880"
     CDS             complement(2335..2868)
                     /gene="lspA"
                     /locus_tag="AC16BC_11880"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3242359.1"
                     /translation="MNSYGISPPARLWRPGLAATIIAAILVVSDQATKVVIQRTMMLH
                     ETIPVMPSVSLTYTRNPGAAFSLLADAHPTFRAWFFLAVSVVALILIAVFLRRVERGD
                     WWSLTALSLILGGAIGNLLDRVRYGEVVDFIDLYVGQYHWPVFNVADSGITVGMLMLL
                     GHALLHRQPDVRPADDP"
     gene            complement(2865..5735)
                     /gene="ileS"
                     /locus_tag="AC16BC_11885"
     CDS             complement(2865..5735)
                     /gene="ileS"
                     /locus_tag="AC16BC_11885"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR3242360.1"
                     /translation="MADSSKTYRDTLNLPTTSFPMRADLARQEPALLARWETDGLYAS
                     LREKRRGAKKFVLHDGPPYANGHIHIGHALNKILKDVIVKYRTMCGDDAPYIPGWDCH
                     GLPIEHQVLKDLGPKKAGMSVLEIRRRCREYAERFVDIQREEFKRLGGLGDWNHPYLT
                     MLPDYEAAIVREFGKIVDTGTVYKGKKPVLWCAYDETALAEAEVEYADHTSPSIYVKF
                     PVSTSIPELPLSTPSKLLPAQFGIQPPSNVRSWSLLIWTTTPWTLPANQAVCMHRNYD
                     YEFILNGDEIFVVASPLVDRVAKELGWTSYQRLGTSKGHLFNNLDCLRPFGEGKSLVV
                     MGDFVTLDQGTGIVHIAPGHGQEDYGVGVEYGLAVYAPVDSRGRFTDDVGTLSGKKVF
                     EANPVIIEMLRTQGRLLHASQISHSYPHCWRCKNPVIFRATEQWFIAMDQPDRTGTTL
                     RQRALKEIERVTWVPKWGRERIVGMMANRPDWCISRQRVWGVPIVAFTCMDCGASLVG
                     KSIADHVADLMEQGDGSDIWFSKSADELLPAGTTCATCGGRRFQQERDILDVWFESGV
                     SHAAVLKRRPELAWPAALYLEGSDQHRGWFHSTLLTSLLTDPRAPYDTVLTHGFVVDG
                     QGKKMSKSAGNVMAPQEIIKQHGAELLRLWVAAEDYRDDIRISPAIMSQLVEAYRKIR
                     NTSRFLLANVEDFDPRRDALPIDRLLPIDRWALHRLAALITRAREAYDAYEFHTVFHA
                     VNNFCAVDLSAMYLDILKDRLYTEASTGPARRAAQTTIEMMLSALVRVMAPILSFTAE
                     DIWTHLPEGSRATASVHLSDFPEPPPTWTLAPDEAARWDRLFEVRSHVAKALERARAG
                     KVIGASLDARVTLYPERSLYDALTRDFRDLPSLLIVSDVALEPIDRRPADAETMTPDL
                     AVVVDRAPGAKCARCWTYRTDVGVDQQRPDVCGRCAGVLAGS"
     gene            complement(5769..6503)
                     /locus_tag="AC16BC_11890"
     CDS             complement(5769..6503)
                     /locus_tag="AC16BC_11890"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3242361.1"
                     /translation="MILAAGLGTRLRPLTDRLPKPLLPIAGRPMIEYTLGWLAAAGIH
                     HVMINLHHKGELIRQALDSGEQFGMTISYSDEPVILGTGGGLKRAERFFDHEPFLIVN
                     ADVLTALDPTAVIQAHAAHRPLATLVVRRDPDVAAYGALGLDAAGRIRRFLGRGAGAD
                     EPLTDVMFTGIHVLDPSVLAGIEADRFSPITDQYIAIVERDAPLRGLLTDALWLDIGT
                     PERYEEAQRLVASGAIPPWPSRLRSR"
     gene            complement(6509..>6954)
                     /locus_tag="AC16BC_11895"
     CDS             complement(6509..>6954)
                     /locus_tag="AC16BC_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR3242362.1"
                     /translation="ELAAAPVVFVHRDYHSRNLMLSGDRLRVIDFQDALMGPRTYDLA
                     SLLRDSYVTLDPALVSRLIDHYLASTPGPRPDAAAFQRLFDVTGLQRNLKAAGRFVYI
                     EKVKGRPTHLPYVTPTLRSARRVLDRYPDLAELRGLLAPYVPEFT"
BASE COUNT         1212 a         2171 c         2370 g         1201 t
ORIGIN      
        1 gtgtcgcagc gctgtcacgc tcggtggtcg aggcggccgc ggccgcgacc tcgacgaagg
       61 cgccaccgct ggtcgcgatc gatcaggaag gcggacgcgt cagtcgcctg cccccgccgt
      121 ttacccagct tcccccggcc gcggcgttgg gcgcgcgcaa ggcggtggcg ctcacgtatg
      181 cgatcgggga gatcatgggc cgtgaattag ccgcgatcgg cgtgaacatg aacatggcgc
      241 cggtgttgga cgtcaacacc aacgcggcca atccgatcat cggcgaccgc gcgttcggcg
      301 acagccccat gatcgtggaa gagcacggcc tggccttgat ggtggggctt caggaccatg
      361 gggtgatcgc gtgcgcgaag cattttccgg ggcacggcgc gacttcggcc gactcccact
      421 tggagttgcc cgtggtggac gcgtcgctcc ggcaactcga acggatccat ttgcgtccgt
      481 tcgcgcacgc catcgccaac cggctgtccg cgctgatgac ggcccacgtc cggtatcccg
      541 cgttggatgc gcggtcgccc gccaccttgt ccaagaagat cgtgacgggc ctgctgcggc
      601 gcgccatggg gttcgacggg gtggtgatca ccgacgatct ggagatgaag gcggtcgcgg
      661 atcggcacga cgcggggagc gccgcgctca aggcgttcca ggccggggcg gacgtgctgc
      721 tcttctgccg cgatcaggac gcgcccgaac aggcgatcga cgcgctggcg gacgccgtca
      781 agcgcaagcg gctgtccgag gcgcgggtcg accagtcgtt gaaccggatt ttgcggctca
      841 aggagcggtt tttgctcggc gccaagccgc cgagcgcgtc acggctgcgc gacatcgtgg
      901 ggtgcgaggc gcacctccgc ctggttgcgc agatcaaatc ctcctgagtg acagatcggg
      961 gcggccgcct tgccgcgccc ggcacgagtg ctcgctccca ggggcgggag cgcgtgcgac
     1021 aaggcgggtt acttccgctt cgaggacttg ccggtcagca gccaatccac gctgaccttc
     1081 ccgaaatccg cgatccgaag cagcagttcc gggttcggca cccgtccgcg ctcgtagcgg
     1141 ctgatgtagt tctgtttttt cacgccgatc gccttggcga attcggtctg ggtcatcccc
     1201 ccgcggattt cccggattcg ctgcccaacc tgggtgggtg aatacttctg catcgccgac
     1261 ctcctcctga ccggaatatg ctcaccgcgg atatacacaa acgtgctggc agtatagcga
     1321 acgcccgaaa agcaagtcaa gacaatggcc ggccgggatg acccgcggag gccgtcagaa
     1381 cgcgccgcgc ggcctgcatg tcctccggca tgggggcggt gaacaccatc gggcgaccgc
     1441 tgatcggatg gatgaacccc agttgttgcg cgtgcagcaa ctgccgatgg accgggacgt
     1501 cgggaggcgg gaccgcgcga cgtcccccgt agaccgggtc gcccaagacg ggaaacccca
     1561 ggtgcgcgaa gtggactcgg atctggtggg tgcgcccggt ctccagcgtc accgcgacca
     1621 aggtcgcccg cccgtagcgc tccacgactt cgtaatggct caccgcgtgc ctggggctgg
     1681 tggtccgggc cgagattttc ttgcgttccc acacgtcccg cccgatcgcc agatcgatgg
     1741 tgcccttgcg cggtcgcacg tcgcccgcca cgatcgccag gtaccgtctg gcaatgctgt
     1801 gcgccttgaa ctgccgcatg agcccggtgt gcgcggcgtc gtgtttggcc gccaccagca
     1861 ggccggaggt gtccttgtcc agtcgatgca cgatgccggg tcgcgcctgt cctccgatgc
     1921 cggccaggtc tcggcaatgg tgcaacaggg cgttgaccag ggtcccgccg gcatggcccg
     1981 gcgcggggtg cacgaccaga cccgcgggtt tgtccaacac caacagatcc tcgtcctcgt
     2041 acacgatgtg caagggaatc ggttcggcgc ggacctccac gggttgcggg ggcggatggc
     2101 agatcacgat ccggtcgccg ggccgaatga ggtatgacgc cttggtccgg cggccgttga
     2161 cctcgatgag cccctcgtcg atgccgcgct ggatgcgcgc tcgcgacaac ggcagtccct
     2221 ggcgcgcgag aaaccgatcg atccgctccg cggcttctcc cgcggtcacg gtgatgagcg
     2281 tggacgcggc cgaggcctct tggggagcgc cgccggctga cgatgccacg ggactcaggg
     2341 gtcgtcggcg ggccggacgt cgggctggcg atgcaggagg gcgtggccca gcaacatcag
     2401 catgcccacc gtgatcccgg agtccgcgac gttgaagacc ggccagtgat actggccaac
     2461 gtacaggtcg atgaaatcca cgacctcgcc gtatcggacc ctgtccagga gattgccgat
     2521 cgcgccccca aggatcaggg acaacgcggt gaggctccac cagtcgccgc gttccacccg
     2581 gcgcagaaag accgcgatga ggatcaacgc cacgaccgag accgcgagga agaaccatgc
     2641 gcgaaacgtc gggtgggcgt ccgcgagcag gctgaacgcc gcgccggggt ttctggtgta
     2701 cgtcagactc accgaaggca tgaccggaat cgtttcgtgg agcatcatgg tgcgctggat
     2761 cacgactttg gtcgcctgat ccgaaaccac gaggatcgcg gcgatgatgg tggcggcaag
     2821 accgggacgc cagaggcgcg cgggcgggga gatcccgtaa gagttcatga gccggcgagc
     2881 acgcccgcgc accgcccgca cacgtccggg cgctgttgat cgacgcccac atcggtccga
     2941 tacgtccagc agcgggcgca cttggccccg ggcgcgcggt cgaccaccac ggcgaggtcg
     3001 ggcgtcatag tctcggcgtc tgcgggccgt cggtcgatcg gctcgagcgc gacgtcggac
     3061 acgatgagca gtgaggggag gtcgcggaaa tcgcgcgtga gcgcgtcgta caacgagcgc
     3121 tccgggtaca gggtgacgcg ggcgtccagc gacgcgccga tcaccttccc ggcccgcgcg
     3181 cgctccaagg ctttggcgac gtgcgagcgg acctcaaaga gccggtccca gcgagcggct
     3241 tcgtcgggtg caagcgtcca ggtcggcggc ggttcgggaa agtcgctgag gtgcacgctg
     3301 gcggtcgcgc gcgatccctc ggggagatgc gtccagatgt cctcggcggt gaacgacaag
     3361 atcggcgcca tcacgcgcac aagcgcggac agcatcatct cgatcgtggt ctgcgcggcg
     3421 cggcgggccg ggccggtgct cgcctcggtg tagagccgat ccttgagaat gtcgaggtac
     3481 atcgcgctca ggtccacggc gcagaaattg ttcacggcgt gaaacacggt atggaactcg
     3541 taggcgtcgt atgcctcacg tgcgcgcgtg atcagcgccg ccaggcggtg caatgcccag
     3601 cgatcgatgg gcaggagccg gtcgatcggc aatgcgtcgc gacgcggatc aaagtcctcg
     3661 acgttggcaa ggagaaaccg cgacgtgttg cggatcttgc ggtaggcctc cacaagctgg
     3721 ctcatgatcg cgggcgaaat gcggatatcg tcccggtagt cctcggccgc cacccagagc
     3781 cggagcagtt cggcgccgtg ttgtttgatg atttcctggg gcgccatgac gttgcccgcg
     3841 gatttggaca tcttcttgcc ctggccgtcc accacgaatc cgtgggtcag caccgtgtcg
     3901 tagggcgcgc gcgggtccgt gagcaacgag gtcaggagcg tgctgtgaaa ccacccgcgg
     3961 tgctggtcgg acccttccaa atagagggcc gcgggccagg cgagttccgg gcgtcgtttg
     4021 agcaccgcgg cgtggctgac gccggactcg aaccacacgt cgaggatgtc gcgttcctgt
     4081 tggaagcggc ggccgccgca tgtggcacag gttgtcccgg cgggcagcag ctcatccgcg
     4141 gattttgaaa accagatgtc gctgccgtcg ccctgctcca tgagatccgc cacgtggtcg
     4201 gcaatcgact tcccgaccag cgacgcgccg caatccatgc aggtaaaggc gacaatgggc
     4261 acgccccaga cccgctgccg ggagatgcac cagtcgggcc ggttcgccat catgcccacg
     4321 atgcgttcgc gtccccactt gggcacccag gttacccgct caatctcttt gagcgcgcgt
     4381 tgccgaagcg tggtgccggt acgatccggt tgatccatgg cgatgaacca ctgctcggtg
     4441 gcgcgaaaga tcaccgggtt tttacaccgc caacagtgcg ggtaggagtg ggagatttga
     4501 gacgcatgaa ggagacgtcc ctgagttcgt agcatttcga tgataactgg attagcctcg
     4561 aatactttct tgccggatag cgtgccgaca tcatctgtga agcggcctcg actatcgacc
     4621 ggggcgtaga cagcgagccc atattccacg ccgactccgt agtcttcttg gccgtgtcct
     4681 ggtgcgatat gtacgatacc agtaccttgg tccagagtga caaaatctcc catcacaacg
     4741 agggacttac cttccccaaa cggccgcaag caatcaaggt tgttaaatag atgccctttg
     4801 ctcgtgccta gtcgttggta cgacgtccat cccagttctt tagcaacacg gtctaccagg
     4861 gggctagcta cgacgaagat ctcgtcgccg ttgagaataa actcatagtc gtaattgcga
     4921 tgcatacaga cagcctggtt tgcaggcaat gtccagggag tcgttgtcca gatgagtaag
     4981 gaccaggaac gcacgttaga aggcggttga atgccgaatt gagctggtag caatttactt
     5041 ggggtagaca ggggtagttc aggtatggat gtcgaaaccg gaaacttcac atagatcgac
     5101 ggcgaggtat ggtcggcgta ttcgacctcg gcttcggcga gcgcggtctc gtcgtacgcg
     5161 caccacaaca cgggtttttt gcctttgtag accgtgccgg tgtccacgat ttttccgaac
     5221 tcgcggacga tcgcggcttc atagtccggc aacatggtca ggtaggggtg gttccaatca
     5281 cccaagccgc cgagccgctt gaactcctcg cgttggatgt ccacgaaccg ctccgcgtac
     5341 tcgcggcagc gccggcggat ttccagcacg ctcatacccg ctttcttggg gccgagatcc
     5401 ttgagcacct ggtgttcgat cggcaggccg tggcagtccc accccgggat atacggcgcg
     5461 tcgtcgccgc acatggtgcg atatttgacg atgacgtcct tgaggatctt gttgagggcg
     5521 tgcccgatgt ggatgtgacc gttggcgtac ggcggtccgt cgtggagcac gaatttctta
     5581 gcgccacggc gtttctcgcg cagcgacgcg tagagtccgt ccgtctccca gcgcgcgagc
     5641 agcgccggct cctgccgcgc gagatcggcc ctcatgggga acgacgtggt cggcagattg
     5701 agcgtgtcgc ggtacgtttt ggaggaatcg gccattcggg cgatcctagc aagcgccgcg
     5761 aaggcgtgtc aacgcgaacg cagtcgggac ggccacggcg ggatggcgcc cgaggcaacc
     5821 aggcgttgcg cttcctcata tcgctcgggg gtgccgatgt cgagccacag ggcgtcggtg
     5881 agcaggcctc ggagcggggc gtcccgttcc acgatcgcga tgtactggtc ggtgatcggc
     5941 gaaaaccgat ccgcttcgat cccggcgagc acggacggat cgagcacgtg gatgccggtg
     6001 aacatcacgt ccgtcaacgg ctcatctgcg ccggcccctc gcccgaggaa gcggcggatc
     6061 cgacccgcgg cgtcaaggcc cagcgcgccg tacgccgcca cgtccggatc gcgacgcacc
     6121 accagcgtgg ccagcgggcg gtgggcggcg tgtgcctgga tgaccgcggt cgggtcgagc
     6181 gcggtaagca cgtcggcgtt gacgatcaag aacggttcgt ggtcaaagaa ccgctccgcc
     6241 cgtttgaggc ctccaccggt acccaagatc acgggttcgt ctgaatagga aatcgtcatc
     6301 ccgaactgct cgccggagtc caaggcttgt cggatcagct cacccttgtg atgcaagttg
     6361 atcatcacgt ggtggatgcc ggccgccgcc agccagccga gggtgtactc gatcatgggc
     6421 cggcccgcga tcgggagcag gggcttgggc agccggtcgg tcaggggacg aaggcgcgtc
     6481 cccagcccgg cggccagaat catggccttc acgtgaactc gggcacgtag ggggcgagca
     6541 ggccccgcag ttccgccagg tccggatagc ggtccaagac ccgacgggcc gaacgcagcg
     6601 tgggcgtgac atacggcaga tgggtgggcc gccctttgac cttttcgatg tagacgaacc
     6661 gccccgcggc tttgaggttg cgctgcagac cggtgacgtc gaagaggcgt tgaaacgccg
     6721 cggcgtctgg gcgcggcccc ggcgtcgagg cgaggtagtg atcgatcagg cggctgacca
     6781 gggcgggatc caacgtgaca tacgagtcgc gcagcagcga ggccaggtcg taggtccgcg
     6841 ggcccatcag cgcgtcctga aagtcgatga cgcggagccg atcgccggag agcatcaggt
     6901 ttcggctatg gtaatcgcgg tgcacgaaga ctacgggcgc cgcggccaac tcat
//
LOCUS       DBNGTT010000515         7647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2677689, whole genome shotgun sequence.
ACCESSION   DBNGTT010000515 DBNGTT010000000
VERSION     DBNGTT010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2677689"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="AC16BC_11900"
     CDS             <1..652
                     /locus_tag="AC16BC_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242363.1"
                     /translation="FGEQNLVTAGLTNRVTSSGAGGAGSWEPLWVRLTQTLRFSHRPS
                     GAAWSALRGEAVIRTQRALQLEIDGFYDHAKGRLVAFDTDARLLLGSYGDIELGRRST
                     RPTGALPRRGDLLDPLAMGAADPGPRTETDYYTVLAHAYLPGGFMLANKTYYNRQAGA
                     YTEIDYGLLYKAQCWSVTLTYQDLPDRNEFGVMFTLIGGTGVDSKAMAGLFDRLAP"
     gene            complement(666..1847)
                     /locus_tag="AC16BC_11905"
     CDS             complement(666..1847)
                     /locus_tag="AC16BC_11905"
                     /inference="COORDINATES: protein motif:HMM:NF020084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HIR3242364.1"
                     /translation="MLKVGVGASIEKATIPAASAAARQAMERAGIVQADFALVFATIP
                     HASLYPRMLERIAEITRTRHVVGCSAMGIITSEGEVEDDPAVAVLALASDTVVATPFL
                     VRSLRHQGGVAARRMVGMLNASRSPRDVVMLFPDTLSFQAGPFLDGLCRAWPGVAAVG
                     GGASENGTHMHTYQWCGTEVASDAVCGALFSGGVSRAIGHTTACHPVGEPLLVTRAEG
                     NIIFEFGGRPAYEVFTRMLRDWSITDLRTAAGLFFLGFPCDPTHATLAPGEYYVRNIH
                     GVDPHEGSLRAGSPVREGQVVSFTIRDADRARRDAEGMVADLATVCAGRPPGFGLYVN
                     CCGRGTSLYGHPNVDVDIIRREFPDLPLIGFFSYAEIGPSADGCCLHNYTGVLTLIGE
                     S"
     gene            2015..2629
                     /locus_tag="AC16BC_11910"
     CDS             2015..2629
                     /locus_tag="AC16BC_11910"
                     /inference="COORDINATES: protein motif:HMM:NF047071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HIR3242365.1"
                     /translation="MTAISDVGTKGERTRRRIVESARSLFHARGYGHTSMDDIVRKSR
                     VTKGNLYYYFESKEALAHAVVELTIAEQFASGPGAGDGGPAEQIVAMFRRAERALSTG
                     KCKGGCLFGNLALEISDVHDGLRQTLDQAFATWERRVAALLTQGIRDRVLSRDLDPKA
                     TARFVVATLEGGILLAKVTRDVGALKACTAMVQRVLEGFQETSR"
     gene            2626..3402
                     /locus_tag="AC16BC_11915"
     CDS             2626..3402
                     /locus_tag="AC16BC_11915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3242366.1"
                     /translation="MTTGGSMRLRDKVAVVTGGGTGIGEAIARRFAAEGARVAITGRR
                     RDALDRVVAGIERAGGRGVALPGSVADERDVRDAVAATLRTFGRIDILVNNAGNLFHA
                     GPLHETTDQVWQDTLDVFLTGVFRFSRAVIPHMLAQGSGAILNISTVSALKAMPGFPA
                     HAYAAAKAGVNMLTKTIAIQYARQGIRCNAICPAGVDTPGVAAVTADPVSRKGFDAMH
                     PVGRMGKPEEIAAAAAYLVSDEAVWTTGVILAVDGGVTAQ"
     gene            3544..3735
                     /locus_tag="AC16BC_11920"
     CDS             3544..3735
                     /locus_tag="AC16BC_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242367.1"
                     /translation="MECVKCHGLMVPDRFVDIEESGEREFGGWRCLVCGNITDPVIVA
                     NRIQPPSPRRALRKRQVAA"
     gene            3811..4344
                     /locus_tag="AC16BC_11925"
     CDS             3811..4344
                     /locus_tag="AC16BC_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242368.1"
                     /translation="MGGKKNVVFGLFYLVLTALLGPYMATTYFGPLQEAEQAHAAAVT
                     ELNRAVEAGAEGQIVQAQTDAILSFNRQQTAAEPIDNIKSGPHAHGNLEALLNIVVGV
                     LIGFLAVASWWKETISWLFIGGALLHSGMLYLGTVLGQGWAWGVLGTGIGPMMILAGL
                     LVAGAASVKGFEARPAR"
     gene            4354..5331
                     /locus_tag="AC16BC_11930"
     CDS             4354..5331
                     /locus_tag="AC16BC_11930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3242369.1"
                     /translation="MAHAAVIERTGEPDVLVWRERPVPEPGPGEALVRHTAVGLNFID
                     VYHRRGIYPLPALPAVIGSEAAGRVEAVGPGVREVAVGDRVAYGGGPIGSYSEARVVP
                     ANRLVKLPDGIDDQRAAAMMLKGMTAEYLLRRTCRVEAGQTILVHAAAGGVGLIACQW
                     AAALGATVIGTVGTREKAALAAAHGCAHPIVTSEGDFVARVKSLTQGRGVPVVYDSVG
                     KDTFMRSLDCLSPRGMMVSFGQASGMVPPLDITTLSAKGSLYLTRPTLFTYTATREDL
                     AASASALFDVVVKGVVRIEVGRTYPLREVARAHADLEARRTVGSTVLIP"
     gene            5328..5894
                     /locus_tag="AC16BC_11935"
     CDS             5328..5894
                     /locus_tag="AC16BC_11935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RBBP9/YdeN family alpha/beta hydrolase"
                     /protein_id="HIR3242370.1"
                     /translation="MNHAASPGRPILIIPGWGDSGPGHWQTLWEAAWPTARRVTQRDW
                     DRPAQADWVATLDHAIADCAEPPILAAHSLGCLAVIHWAATHRRPVAGALLAAPVDSE
                     RKEFPPTITGFAPVPTGPLPFPSVVAASVNDPFMQHIRARALADAWGSRFVDAGRSGH
                     INTASGFGPWPEGEALLRELIAGTGAPG"
     gene            5975..6820
                     /gene="ispH"
                     /locus_tag="AC16BC_11940"
     CDS             5975..6820
                     /gene="ispH"
                     /locus_tag="AC16BC_11940"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR3242371.1"
                     /translation="MEVEVAKHSGFCYGVKEAIDLATETAASSERPTVTLGPLIHNPQ
                     EIERLNRDYGIERVESLDGLRDVNVVIRAHGVPPEYFDRAAAQQLTVIDATCRFVTDV
                     QNAAIEYCRRGYPLYVVGEPKHPEVIGILGHARRAYPNAEIHVVEQVEEIINANPERA
                     AVVFQTTHEYAKYKLLERHIKERKLPWKIKNTICGATKSNQYAADELARRVDVMVVIG
                     GKNSGNTRRLMDLCETYTRAYHVETAEELNTAWFAGAKKVGVTAGASTPEWIVDDVVS
                     AIESL"
     gene            6987..>7647
                     /locus_tag="AC16BC_11945"
     CDS             6987..>7647
                     /locus_tag="AC16BC_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR3242372.1"
                     /translation="MRRSISTTARIALAAAGIVGAILLILTVLKSPVIFGLREDIARA
                     IPAQAVPAGAGGLSAEACGTCHREIYEEWKTSIHSQAFVDPFFQAYWRKDKHIWVCLN
                     CHTPLQNQQAEVVAWRPGVRVDRAEKRSNPDFDEALRHEGITCAACHVRDGVIEGPFE
                     DAAAPHPTRYHPRFLSTDICYTCHSVPSDTLQFYNGGPCATFMEYEAGPYKAKGYICQ
                     NC"
BASE COUNT         1272 a         2390 c         2769 g         1216 t
ORIGIN      
        1 gttcggcgaa cagaatctgg tcaccgcagg gctgaccaat cgcgtgacgt catcgggcgc
       61 cggcggcgcc gggtcgtggg agccgctttg ggtgcggttg acccagacgc tccgcttctc
      121 gcaccggcct tcgggcgcgg cgtggtccgc gttgcgcggc gaggcggtca tccggacgca
      181 acgcgcgctg caactcgaga tcgacgggtt ctacgaccac gccaagggcc ggctcgtggc
      241 gtttgacacg gatgcgcgac ttctcctcgg atcgtacggc gacatcgaac tcgggaggcg
      301 ctccacgcgg ccgaccgggg cgctgccccg ccgaggggat ctgttggatc cgttggcgat
      361 gggcgccgcg gacccggggc cgcgcaccga gaccgactat tacacggtcc tggcgcacgc
      421 gtaccttccg ggcggcttca tgttggccaa caagacctac tacaaccgcc aggccggcgc
      481 gtacaccgaa atcgactacg gtctgctgta caaggcgcag tgttggagcg tcaccctgac
      541 ctatcaggat ctgccggatc gcaacgagtt cggcgtgatg ttcaccctga tcgggggcac
      601 cggggtggat tccaaggcga tggccggtct cttcgaccga ctggcgccct gacgcgacgg
      661 cgcgttcagg attctccgat caacgtcagc acgccggtgt agttgtgcag gcagcagccg
      721 tcggccgacg ggccgatctc ggcgtagctg aaaaatccga tcaacggcaa gtcggggaat
      781 tcccggcgga tgatgtcgac gtccacgttg ggatggccgt acaacgacgt gccgcggccg
      841 cagcaattga cgtagagtcc gaagcccgga gggcgtcccg cgcacacggt ggcgagatcc
      901 gccaccatgc cctccgcgtc ccgtcgcgcg cggtccgcgt cgcggatcgt aaacgagacg
      961 acctggccct cgcggaccgg cgaccccgcg cgcagcgagc cttcgtgcgg gtccacgccg
     1021 tggatgttgc gcacatagta ttcgccgggc gccagcgtcg cgtgcgtcgg gtcgcagggg
     1081 aatcccaaaa agaacagccc ggcggcggtg cgcaggtccg tgatggacca gtcgcggagc
     1141 atccgggtga agacctcgta ggcgggccgg ccgccgaact cgaaaataat attgccctcc
     1201 gcccgcgtga ccaacagcgg ttccccgacc gggtggcagg ccgtggtgtg tccgatcgcg
     1261 cggctcacgc cgccggaaaa cagggcgccg cacaccgcgt cggaggcgac ctcggtgccg
     1321 caccactggt acgtgtgcat gtgcgtgccg ttttccgacg cgccccctcc caccgccgcc
     1381 acgccgggcc aggcgcggca gaggccgtcg aggaagggcc cggcctggaa cgagagcgtg
     1441 tcgggaaaga gcatcaccac gtcccgcggc gagcgcgacg cgttgagcat gccgaccatg
     1501 cggcgggcgg cgacgccgcc ctgatgccgg agggaacgca ccaaaaacgg cgtggccacc
     1561 acggtgtccg aggccaaggc caggaccgcg acggccgggt cgtcttcgac ctcgccctcc
     1621 gacgtgatga tgcccatcgc gctgcacccg accacgtggc gggtgcgggt gatctcggcg
     1681 atgcgctcca gcatccgggg gtacagcgag gcgtggggga tcgtggcgaa cacgagcgcg
     1741 aagtcggcct ggacgatccc ggcccgttcc atggcttgtc gcgccgcggc gctggccgcg
     1801 gggatggtcg ccttctcgat cgacgcgccg actccgacct tgagcattcg cttccggttg
     1861 accgcgacgg gaccagttca tcctaacagg ttgcgttccg cggtggcaac gagcggcgcg
     1921 catcagcgcg catcagatcg aagcggcggg gtattgacag tcccatggcc gttggagtat
     1981 aaaatagact gactggtcgg ttttatatga cgagatgacc gccatatcgg atgtcgggac
     2041 gaaaggcgaa cggacgcgcc ggcgcatcgt cgagagcgcc cggtcgctgt tccacgcccg
     2101 gggctacggc cacacgagca tggacgacat tgtccgcaag tcgcgggtga ccaaaggaaa
     2161 cctgtactac tactttgaga gcaaggaggc gttggcccac gcggtggtgg agctgaccat
     2221 cgccgagcaa ttcgcatccg ggcccggtgc cggggacgga gggccggccg aacaaatcgt
     2281 ggcgatgttc cggcgggcgg agcgggcatt gtcaaccggc aagtgcaagg gcggctgctt
     2341 gttcggcaat ctggccctgg aaatttcgga tgtgcacgac ggattgcgtc aaacccttga
     2401 ccaggcgttt gcgacctggg aacgacgggt ggccgcgctg ttgacgcagg ggattcggga
     2461 ccgtgtgttg tcgcgcgacc tcgaccccaa ggccacggcg cgcttcgtgg tggccacgct
     2521 ggaaggcggg attttactcg ccaaggtgac ccgggacgtg ggggcgctga aggcctgcac
     2581 cgcgatggtc cagcgcgtgc tggaaggatt tcaggagacg tcgcgatgac cacgggagga
     2641 tccatgcggc ttcgagacaa ggtggcggtg gtcacgggcg gcggaaccgg aatcggcgaa
     2701 gccatcgcgc gccgcttcgc ggcggagggc gcccgggtgg cgatcacagg tcgccgtcgg
     2761 gacgcgttgg atcgggtggt cgcgggaatc gaacgggccg gcggacgggg ggtcgcgttg
     2821 cccggaagcg tggcggatga acgggacgtg cgggacgcgg tggcggccac gctgcggacc
     2881 ttcggccgga tcgacattct cgtcaataac gcgggcaatc tgtttcacgc cggccccttg
     2941 cacgaaacca cggaccaggt gtggcaggac acgctcgacg tgttcttgac gggcgtgttc
     3001 cggttcagcc gcgccgtcat cccgcacatg ctcgcgcaag ggagcggggc gatcctcaat
     3061 atctccacgg tgtcggcgct caaggccatg ccggggttcc cggcgcacgc gtatgcggcg
     3121 gccaaagccg gcgtgaacat gttgaccaag accatcgcaa tccagtacgc gcggcagggc
     3181 atccgatgca acgcgatctg tccggcgggg gtggacacgc cgggcgtggc cgcggtcacg
     3241 gccgatcccg tctcgcggaa gggcttcgac gcgatgcatc cggtcgggcg gatggggaag
     3301 ccggaggaga tcgccgcggc cgccgcgtac ctggtgtcgg acgaggcggt gtggactacg
     3361 ggcgtgattc tcgcggtgga cggcggggtg accgcccaat gaaccgcggg acgcggcgcc
     3421 gagcgaagac acggcgggcg tgggtcgtat tactggagac ggaaggagaa agtacaagga
     3481 tctcgtggcg acgggctggc gcccgtcacc acgcttcgca agcgaggagg gaggtgagga
     3541 cggatggaat gcgtgaaatg tcatggatta atggttcctg atcggttcgt ggacatcgag
     3601 gaaagcgggg aacgcgaatt cggcggctgg cgctgtctgg tgtgcggcaa catcaccgac
     3661 ccggtgatcg tggccaaccg gatccagcca ccgtcgccca ggcgggcgct gcggaagcgg
     3721 caagtcgccg cttaagcagg cccccggggc gcgtacgtcc ggcggacggt tttgagggat
     3781 cggtgcgact gacaccacgg gagggatgtc atgggcggaa agaagaacgt cgtgttcggc
     3841 ttgttctatc tggtgttgac cgcactgttg ggaccgtaca tggccacgac gtatttcggg
     3901 ccactgcaag aagcggaaca ggcccatgcg gccgccgtga ccgagttgaa tcgcgcggtc
     3961 gaggcgggcg cagaggggca gatcgtgcag gcacaaaccg acgcgattct ctcgttcaac
     4021 cggcaacaga ccgcggccga accgatcgac aacatcaaga gcggcccgca cgcgcacggc
     4081 aatctggagg cgctgctgaa catcgtggtc ggggtcttga tcgggttcct ggccgtcgca
     4141 agctggtgga aggaaacgat cagttggctg ttcatcggcg gagccctgct gcactccggg
     4201 atgttgtatc tggggaccgt gttgggccaa ggatgggcgt ggggcgtgtt gggcaccggc
     4261 atcggtccga tgatgatcct ggccgggttg ctcgtggccg gcgcggcgag cgtgaaaggc
     4321 tttgaagcgc gcccggcgcg ctgaggtcac gggatggcgc acgcggcggt gatcgaacgc
     4381 accggggaac cggacgtgct ggtgtggcgc gagcggccgg tgccggagcc ggggcccggc
     4441 gaggcgctgg ttcggcacac ggcggtcggc ctgaatttca tcgacgtgta tcaccggcgg
     4501 gggatctacc ccctgccagc gctgccggcg gtcatcggtt cggaagcggc gggccgcgtc
     4561 gaggccgtgg ggcccggagt ccgcgaggtg gcggtgggag atcgcgtcgc gtacggggga
     4621 ggaccgatcg gctcgtacag cgaggcgcgc gtggtgcccg cgaatcggct ggtgaagctg
     4681 cctgacggga tcgacgatca gcgcgccgcc gcgatgatgc tcaagggcat gaccgccgag
     4741 tacctgctgc gccggacctg tcgcgtcgag gcggggcaga ccattctcgt gcatgccgcg
     4801 gccggagggg tcgggttgat cgcgtgccag tgggccgcgg cgttgggggc gacggtgatc
     4861 ggcacggtgg gcacgcgcga aaaggccgcg ctcgccgcgg cgcacggctg cgcgcacccg
     4921 atcgtgacgt cggaggggga cttcgtggcg cgcgtgaagt cgctcactca ggggcgcgga
     4981 gtccccgtgg tgtatgactc ggtgggcaaa gacacgttca tgcgttcgct cgactgtctg
     5041 tcgccgcggg gcatgatggt cagcttcggc caggcctcgg gcatggtccc gcccctggat
     5101 atcacgacgc tttcggccaa gggctcgctg tatctgaccc ggccgacgct gttcacgtac
     5161 accgcgaccc gtgaggacct cgcggcgagc gcgtcggcgc tcttcgacgt ggtcgtgaag
     5221 ggcgtggtgc gcatcgaggt cggccggacc tacccgttgc gcgaggtggc gcgcgcgcac
     5281 gccgacctgg aagcgcgccg caccgtgggt tcgacggtgc tcattccatg aatcacgccg
     5341 cgtcgccggg ccgtccgatc ctgatcattc ccggctgggg cgactcgggg cccgggcatt
     5401 ggcagacgtt gtgggaggcc gcctggccaa ccgcgcgccg ggtcacgcag cgggactggg
     5461 accgcccggc ccaggcggac tgggtggcga cgctcgacca cgccattgcg gactgcgccg
     5521 agccgccgat cctggcggcc cactccttgg gctgtctggc cgtgattcac tgggcggcga
     5581 cgcaccggcg gccggtggcc ggcgcgttgt tggcggcgcc ggtggattcc gagcgcaagg
     5641 aatttccgcc gaccatcacc gggttcgccc cggtgccgac cggcccgctg ccgtttccca
     5701 gcgtggtcgc ggcctcggtg aacgatccgt tcatgcagca cattcgcgcg cgggcgttgg
     5761 cggacgcgtg gggcagtcgc ttcgtggacg ccggacgttc cggacacatc aataccgcgt
     5821 cagggttcgg tccgtggccg gagggcgagg cgctcctgcg ggagctgatc gcggggacgg
     5881 gcgctccggg ctgaagcgcg gcccgcgcgg cttgcaatca gccgtccccg ccgatatact
     5941 ggccccggat tttgaccgat cgcgagatcc gcgcatggaa gtcgaagtcg ccaaacattc
     6001 ggggttttgc tacggggtga aggaggcgat cgacctcgcc accgagaccg cggcgtcatc
     6061 cgagcgtccg accgtgaccc tcggccccct gatccataat ccccaggaaa ttgaacggct
     6121 caaccgggac tacgggatcg agcgcgtcga gtcgctggac ggtctgcgcg acgtgaacgt
     6181 ggtcatccgc gcgcacggcg tgccgccgga gtacttcgac cgcgccgccg cgcaacagct
     6241 caccgtgatc gacgccacgt gccggttcgt gaccgatgtg cagaacgcgg cgatcgaata
     6301 ttgccggcgc ggctatccgt tgtacgtggt gggcgagccc aagcatccgg aggtgatcgg
     6361 catcctgggc cacgcccgcc gggcgtaccc caacgcggag atccacgtcg tcgagcaagt
     6421 cgaagaaatc atcaacgcca accccgagcg cgcggccgtg gtgttccaaa ccacgcacga
     6481 atacgccaag tacaagttgt tggagcgcca catcaaggaa cgcaagctgc cgtggaaaat
     6541 caagaacacg atttgcggcg cgaccaagag caaccagtac gcggccgatg agctggcccg
     6601 ccgggtcgac gtcatggtcg tgatcggcgg caagaacagc gggaacaccc ggcgcctgat
     6661 ggacctgtgc gaaacctaca cgcgcgcgta ccacgtggag acggccgagg aactcaacac
     6721 cgcgtggttc gccggcgcga agaaggtcgg cgtgacggcc ggcgcgtcga cgccggagtg
     6781 gatcgtcgac gacgtggtct cggccatcga atcgctctag agcctgtcca tgaggggcca
     6841 tctgcggcgt tgtcgctgcg cttccggtcc tcacgtacga cccagtacgc tccggtccgg
     6901 ttctcgctcc gccttgcatc tggccgctcc tgaacaggct cccgtccgac gatagtcgga
     6961 cggattgcca gcacacacaa cgcgccatgc ggcggtcaat atccacaacg gcacgaatcg
     7021 cgctggctgc tgctgggata gtcggagcga tcctcctcat cctcacggtg ctcaagtccc
     7081 cggtgatctt tggattgcgc gaggacattg cgcgggcgat tcctgcgcag gcggtgccgg
     7141 cgggggcggg cgggttgtcg gctgaggcgt gcggcacgtg tcaccgcgaa atctacgagg
     7201 agtggaagac ctccattcat tcgcaagcgt tcgttgatcc gttttttcag gcgtactggc
     7261 ggaaggataa gcacatctgg gtctgtttga attgtcacac cccgctgcag aatcagcagg
     7321 ccgaggtggt ggcctggcgt ccgggcgtgc gcgtggatcg agccgagaag cggtccaatc
     7381 cggatttcga cgaggcgttg cggcacgagg gcatcacgtg cgcggcgtgt cacgtgcgcg
     7441 acggggtgat cgagggaccg ttcgaggacg cggcggcccc gcaccccacg cgctaccacc
     7501 cgcgctttct ctccaccgac atctgttaca cgtgccactc ggtgccgtcc gatacgctcc
     7561 agttttataa cggcgggccg tgcgccacgt tcatggaata cgaggccggg ccgtacaagg
     7621 ccaagggcta catctgccag aactgcc
//
LOCUS       DBNGTT010000516         3841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_890975, whole genome shotgun sequence.
ACCESSION   DBNGTT010000516 DBNGTT010000000
VERSION     DBNGTT010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_890975"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /gene="hemA"
                     /locus_tag="AC16BC_11950"
     CDS             complement(<1..1224)
                     /gene="hemA"
                     /locus_tag="AC16BC_11950"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR3242373.1"
                     /translation="MNIIVVGLNHRTAPVAVRERFALPESEWPGALDRLRSAHGVTEG
                     FVLSTCNRVEVCAAVRDTQEGFEGATRFFTAFPGAPGESPVSHLYYYSSADAIRHVFR
                     VAASLDSMVLGEPQILGQVKEAFDAALAHQATGVVLNKLFKKAISVAKRIRTETRVAE
                     SAVSVSSAAVELARKIFRRLEDTRVVVMGSGEMAELAAKSLVSHGAERLAIAARNQER
                     VDALAREYSAQAVARDAFLDELVHADIVICSTGAKHYLLDAAQMADVARRRKGRPLFL
                     IDLSVPRNIDPAVNDLEDVFLYNIDDLNSVVEANRREREREAVKAEAIIDEEIEQMLK
                     WLKSLDVTPTIVDLRRRVEELRRVEVERVLARYRGGAESEEALRAALDGVTTALVNKL
                     LHTPLVVLKDEATQTQ"
     gene            complement(1349..1672)
                     /locus_tag="AC16BC_11955"
     CDS             complement(1349..1672)
                     /locus_tag="AC16BC_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3242374.1"
                     /translation="MSNPLEDEVKGEIARHKIVIYAKGTRTMPRCGFTVETIQFFNKY
                     QHPFEVIDVLENMAKRQFLSQMTNWPTLPKVFINGQFYGDTDVLGEMERKGELEPLLK
                     AAFGE"
     gene            complement(1725..1979)
                     /locus_tag="AC16BC_11960"
     CDS             complement(1725..1979)
                     /locus_tag="AC16BC_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR3242375.1"
                     /translation="MITDEAIQSYLQAALPDANVSIMDRTGTRDHFVIRVVSAAFQDK
                     NLLDRNRMIYQALREPMADGRIHAMELKAQTPEEADAPRA"
     gene            complement(2165..2380)
                     /locus_tag="AC16BC_11965"
     CDS             complement(2165..2380)
                     /locus_tag="AC16BC_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3242376.1"
                     /translation="MTASVLVLASAGCTSPERAAQERFELARFEEQQGNAAHARQIYQ
                     EIIAKYPETSWAEQARQRVGELPSAQP"
     gene            complement(2472..3482)
                     /locus_tag="AC16BC_11970"
     CDS             complement(2472..3482)
                     /locus_tag="AC16BC_11970"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3242377.1"
                     /translation="MQTGRPEVALATMQDVWDFYRTDLQEVEVHIEKYVDSGVPLINQ
                     VALYILTSGGKRIRPLLLMISARLSGFDGPADQAVTLASSIEYIHAATLLHDDVLDDA
                     TLRRGKDPAHAVWGNRATILVGDYLYTKAVCLAVQIGLLEVDAILANACRIMAEGEAL
                     QLLHCGDLSLTDEAYLKVVECKTAALISAACRLGGVISGAPWRAKDALSQFGMHLGIA
                     YQVADDTLDYVADQGRLGKALGKDLSEGKITLPLLHLLRSVNGAERRLVETVVRDGQA
                     SPEQLRDILDLMHTYGSTAYALDVARQHITLAQQHLEEFDNGPHRRALSAVADYVVAR
                     DH"
     gene            3620..>3841
                     /locus_tag="AC16BC_11975"
     CDS             3620..>3841
                     /locus_tag="AC16BC_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242378.1"
                     /translation="MERERLAIPARLIGFIISPVAARVASAEPFTFKPPKAAPNYADM
                     LPKQRMIERSPTTVAGRRAVLIVKTYDPGF"
BASE COUNT          647 a         1298 c         1227 g          669 t
ORIGIN      
        1 ctgggtctgg gtcgcctcgt ccttgagcac gaccaggggc gtatgcagga gcttattgac
       61 cagggcggtg gtgaccccgt ccagcgccgc ccggagcgcc tcctccgact ccgcgccgcc
      121 gcggtagcgg gcgaggaccc gctcgacttc gacccggcgg agttcttcga cgcgccgccg
      181 caggtccacg atcgtcggcg ttacatcgag ggacttgagc cacttcaaca tctgctcgat
      241 ctcttcatcg atgatcgctt cggctttcac cgcttcccgt tcgcgttcgc ggcgattggc
      301 ttccaccacc gagttcaaat cgtcgatgtt gtagagaaag acatcttcca ggtcattgac
      361 cgcgggatcg atgttgcgcg gcaccgagag gtcgatcaga aacagtgggc gccccttgcg
      421 tcgtcgcgcg acgtcggcca tttgcgccgc gtccaagaga tagtgtttcg cgccggtcga
      481 gcagatcacg atgtccgcgt ggaccaactc gtcgaggaag gcgtcgcggg cgaccgcttg
      541 cgcagagtat tcgcgcgcca acgcgtccac ccgctcctga ttgcgcgcgg cgatggcgag
      601 gcgctcggct ccgtgcgaca ccagactctt ggcggccagc tccgccatct cgcccgagcc
      661 catcaccacc acccgcgtgt cctccagacg tcggaagatc ttgcgcgcca actccacggc
      721 cgccgaactg accgacaccg cgctctccgc cacccgcgtt tcggtgcgga tgcgcttggc
      781 caccgagatc gctttcttga acagcttgtt gaggaccacc ccggtggcct ggtgggcgag
      841 cgcggcgtca aaggcctctt tgacctgccc caggatctgc ggctcgccga gcaccatgga
      901 atcaaggctc gcggccacgc ggaagacgtg acggatcgcg tcggcggacg agtaataata
      961 aaggtgggaa accggggatt cgccgggagc cccggggaag gcggtgaaaa aacgggtcgc
     1021 gccctcgaac ccctcctggg tgtcccggac ggccgcgcac acctcgaccc ggttacaggt
     1081 cgagagcacg aacccctcgg tcaccccgtg agcggaacgc aggcggtcga gcgcgccggg
     1141 ccactcggac tccgggaggg cgaaccgctc gcgcaccgcc accggggcgg tgcggtggtt
     1201 cagccccacg acaatgatgt tcacgagcgg ccgtctcctg atcaacccgc cacgtcgtcg
     1261 cggcgcgctc ccgccccttc cccgtcaggc gcggcgcccc gcacgcggtc aggaaggacg
     1321 cgggaaccgg ccgtcccgat gggccgcgtt actccccgaa cgccgccttg agcagcggtt
     1381 cgagttcgcc ctttcgctcc atctcgccga gcacgtcggt gtccccgtag aactggccgt
     1441 tgatgaagac cttgggcaac gtgggccagt tcgtcatctg cgacaggaac tgccgcttgg
     1501 ccatgttctc caggacgtcg atcacttcaa acggatgctg gtacttgttg aagaactgga
     1561 tcgtctcgac cgtgaacccg caccgcggca tcgttctcgt gcctttggcg tagatcacga
     1621 tcttatgtcg cgcgatctcg cccttgacct cgtcttccaa cggattgctc atcgtctcgc
     1681 tccccctctc gaacacaccc cggtcattcg gccgaccacc gcggtcacgc gcgcggcgcg
     1741 tccgcttcct ccggcgtttg cgccttgagc tccatcgcgt ggatgcggcc gtcggccatg
     1801 ggctcgcgca aggcctgata aatcatccgg ttgcgatcca gcagattctt gtcctggaac
     1861 gcggcggaca ccacccggat gacaaaatgg tcccgcgtgc ccgtccggtc catgatcgac
     1921 acgttcgcgt cgggcaacgc ggcttgcaaa tacgactgaa tggcttcgtc ggtaatcact
     1981 gacgttctcc cgtccgacaa tccccggaag gcaccggtcg gctatcttag cgcaatcctc
     2041 gcgcgcgggg caagggcggg gcccagtcca cgctggacgt gggctcctgc ggtcggccgc
     2101 tccggaatgg ccgggtgcaa aactcccatg ggcccagcgg tcggaacccc gccgtccgag
     2161 ccgcttacgg ttgggcggac gggagttcgc ccacgcgttg gcgggcctgt tccgcccacg
     2221 acgtctcggg gtatttggcg atgatttcct ggtagatctg gcgggcatgg gcggcgttgc
     2281 cttgttgttc ttcgaaccgg gccagctcaa accgctcctg cgcggcccgc tcgggcgacg
     2341 tgcagccggc ggacgcaagc accaggaccg aggccgtcac acccaggaga agactccgtc
     2401 gaaggatcag gatttacact ccttgtcagg ggaccaaacg ggcgaagcgg gacgaacgat
     2461 tcacgcgggg gtcagtgatc ccgcgcgacc acgtagtccg cgaccgcgga caatgcgcgg
     2521 cggtgcggac cgttgtcgaa ttcctcaagg tgctgttggg cgagagtgat gtgctgacgg
     2581 gcgacgtcga gggcgtacgc ggtcgacccg taggtgtgca tgaggtccaa aatgtcgcga
     2641 agctgttccg gagaggcctg gccgtcgcgc accacggttt cgacgagccg acgctcggcg
     2701 ccgttcaccg accgtaagag gtgcagcaac ggcagcgtga tcttgccctc ggacaggtcc
     2761 ttgcccaggg ctttgcccag acgcccttga tccgccacgt agtcgagcgt gtcgtccgcc
     2821 acctgatacg cgatcccgag gtgcatgccg aattgactga gggcgtcctt tgcgcgccac
     2881 ggcgcgccgc tgatcacgcc ccccagccgg cacgcggccg aaatcagcgc cgcggtcttg
     2941 cactcgacaa ccttgagata cgcctcgtcg gtgagcgaca gatccccgca gtggagcaat
     3001 tgcagcgcct cgccctcggc catgatccgg cacgcgttcg ccaggatggc gtccacttcc
     3061 aacagcccga tctgcacggc cagacacacg gctttggtgt acagataatc gccgaccaga
     3121 atggtggcgc ggttgcccca caccgcgtgg gcgggatcct tgccgcgccg caaggtggcg
     3181 tcgtccaaca cgtcgtcgtg cagcagcgtc gcggcgtgga tgtattcgat cgaactggcc
     3241 agcgtaaccg cctggtccgc cggcccgtca aacccggaca accgggcgga aatcatgagc
     3301 aacagcggcc tgatccgctt gccgccgctg gtcaggatat agagggcgac ttgattgatg
     3361 agcgggacgc cggagtccac gtatttttcg atgtggacct ctacttcctg caggtccgtc
     3421 cggtagaagt cccagacgtc ctgcatggtc gcgagcgcga cctccggcct gccggtctgc
     3481 atggccgcca gtgtagggga ggctcgcgga tgaagtcaag gagaatcgtg gcgattccga
     3541 cgcgcgggaa agaccgggag tggatgcgac gcatcgcggc gaattgactc tcgagccgag
     3601 ccgtccgtat gatcgccgca tggaacggga gcggctggcg atccccgccc gactgatcgg
     3661 ctttatcatc agcccggtgg ccgcgcgcgt cgcgtcggcg gaacccttca ccttcaagcc
     3721 ccccaaagcc gccccgaatt acgccgacat gctgcccaag caacgcatga tcgagcgcag
     3781 cccgaccacc gtggcgggtc gcagggcggt gctgatcgtc aagacgtacg accccgggtt
     3841 c
//
LOCUS       DBNGTT010000517         4257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1145870, whole genome shotgun sequence.
ACCESSION   DBNGTT010000517 DBNGTT010000000
VERSION     DBNGTT010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1145870"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="AC16BC_11980"
     CDS             <1..134
                     /locus_tag="AC16BC_11980"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012672061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3242379.1"
                     /translation="NTLSLHDALPISVISSRIVNEVKGVNRVVYDISTQPPSTIEWE"
     gene            complement(320..520)
                     /locus_tag="AC16BC_11985"
     CDS             complement(320..520)
                     /locus_tag="AC16BC_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3242380.1"
                     /translation="MARGKVKWFNGSKGYGFIAQDSGSDVFVHFSAIQGDGFKTLDEG
                     QEVEFEIVNGPKGLQAANVVKV"
     gene            complement(749..1876)
                     /locus_tag="AC16BC_11990"
     CDS             complement(749..1876)
                     /locus_tag="AC16BC_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3242381.1"
                     /translation="MEPTTLLIVDDDPNIRELLSTKLIHLGYRCKTANGAERALGLIE
                     REPIHLVLSDIMMPGLTGIDLLKRLVLSYPEIAVVMLTAAADTQVAVQAMKLGAYDYI
                     TKPFNLEELTVHVEKALERRQLLVENRAYHRSLEQRVAEQTREIREHLQLERRRREEI
                     NHTLTLLERSYTDTIDALIMALDYRDNETQGHTQRVSVYTVELAERMGVSAADLETIK
                     RGAVLHDIGKIGVPDTILRKPAKLTDAEWTEMRRHVQYGYGMLKDIAFLRDAAKIVLH
                     HHERYDGLGYPNQASRNEILFGARIFVVADTFDAMTSERPYRKALPYQDARAEILRCA
                     GTQFDPRVVDTFLGVAPVRWQELREEVDVMLAARRTAAAST"
     gene            2125..2200
                     /locus_tag="AC16BC_11995"
     tRNA            2125..2200
                     /locus_tag="AC16BC_11995"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2158..2160,aa:Ala,seq:ggc)
     gene            2579..2908
                     /locus_tag="AC16BC_12000"
     CDS             2579..2908
                     /locus_tag="AC16BC_12000"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3242382.1"
                     /translation="MSLATIDQKLLEKVTNTIVERFHPRRIILFGSRARGEARPDSDV
                     DLFVEMESTRRPPERAIEVSSVFGLRPWPLDVVVYTPDEVKRLRRVRGTLLSVIEREG
                     TVLYECA"
     gene            2895..3185
                     /locus_tag="AC16BC_12005"
     CDS             2895..3185
                     /locus_tag="AC16BC_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR3242383.1"
                     /translation="MSAPESNFAAWVRKAEHDLLNIQNNLASSDVPWDTVCFHEIVQK
                     KRGISADTALRLGRYFTMSPEFWMNLQVHYDLESERDKLAHKLEKTVRVYAA"
     gene            3188..3373
                     /locus_tag="AC16BC_12010"
     CDS             3188..3373
                     /locus_tag="AC16BC_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242384.1"
                     /translation="MARRTRDWNKGLANDLKDTTFMRAFINASLDEGVPVQQVIRKLA
                     VLNRGVRADKASDAKGH"
     gene            3413..3739
                     /locus_tag="AC16BC_12015"
     CDS             3413..3739
                     /locus_tag="AC16BC_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing
                     transcriptional regulator"
                     /protein_id="HIR3242385.1"
                     /translation="MQVTRAFRVTIMERAGRDARFRQHLLIEAINQLLTGDLSAGKAM
                     LRDYINATISFDQLARKLKKSSKSLHRMLGPQGNPTAENLFGMIKVLQAHERVQLHVK
                     SQRSAA"
     gene            3937..>4257
                     /locus_tag="AC16BC_12020"
     CDS             3937..>4257
                     /locus_tag="AC16BC_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HIR3242386.1"
                     /translation="MANRKRVVIPTERIEGQIVLVRGQKVMLDADLAVLYDVQVKALN
                     QAVRRNIERFPEDFMFQLTAEEITRLRSQTVTLKTGDPARMGFDANASGLRSQIVTSN
                     ADDRS"
BASE COUNT          806 a         1318 c         1281 g          852 t
ORIGIN      
        1 ttaacactct ttccctacac gacgctcttc cgatctccgt gatttcatcc cgcatcgtca
       61 acgaggtcaa gggcgtgaac cgcgtggtct acgatatttc gacccaaccg cccagcacca
      121 tcgaatggga ataaccggct gctgaaaacg tccatctgcg gcgttgccgc tgcgcatccg
      181 ctcctcacgt actcacgacg tacgctccgg tgcggtgctc gcggcgcctt gcatctggag
      241 ctttttgagc agccggtcta acggcacaaa aaagcccggg cgacgcccgg gcttttttta
      301 tgggagcaac agacgacgct cagactttga ccacgttcgc cgcctgcaaa cctttcggtc
      361 cattcacgat ttcaaattcg acctcttgac cttcgtccag cgtcttgaat ccgtcacctt
      421 gaatggcgga aaagtgcacg aagacgtcgc tgccagagtc ctgggcgatg aatccatacc
      481 ccttgctgcc gttgaaccac ttcactttgc ctcttgccat tgcccttcac ctcccttcaa
      541 tcaaccccac cccgaatgac ttcgggcgtt acgagccgtc cgtaggaaca acaatgccgc
      601 agggctcgga ccctcaagag cactgcggca caatctcatt ctattccttt ggacaactcc
      661 cgacacctaa ccgacacgga ctatgtagca cgagaaaact cgcggtgtca aggccgggaa
      721 ttcgacggtt ttgacgggcg gcgggcgctt acgtggacgc ggcggcggtg cgccgcgcgg
      781 ccagcatgac gtccacttct tcgcgcagct cctgccaccg gaccggggcc acgcccagga
      841 acgtgtccac cacgcggggg tcgaactgcg tgcccgcgca ccggagaatc tcggcgcggg
      901 cgtcctgata ggggagggcc ttgcggtacg ggcgctccga ggtcatggcg tcgaacgtgt
      961 cggccaccac gaagatgcgc gcgccgaaca ggatctcgtt gcggctcgcc tggttgggat
     1021 acccgagccc gtcgtagcgc tcgtggtggt ggagcacgat cttggccgcg tcgcgcagga
     1081 acgcgatgtc cttgagcatc ccgtagccgt attgcacgtg ccgccgcatc tcggtccatt
     1141 ccgcgtcggt cagcttggcc ggcttgcgga gaatggtgtc cgggaccccg atcttgccga
     1201 tgtcgtgcag cacggccccg cgtttgatcg tttccaggtc cgccgcgctc acgcccatgc
     1261 gctcggccag ctccaccgtg tacacggaca cgcgctgggt gtgcccctgg gtctcgttgt
     1321 cccggtaatc cagggccatg atcagcgcgt cgatggtgtc ggtgtacgag cgctccagca
     1381 gggtgagggt gtgattgatc tcctcgcgcc gccgccgttc gagttgcagg tgctcgcgga
     1441 tctcccgcgt ctgttccgcc acgcgctgct cgagcgagcg gtggtacgcg cggttctcca
     1501 ccagcagttg ccggcgctcc aacgcttttt ccacgtggac cgtcagctct tcgagattga
     1561 acggcttggt gatgtagtcg tacgcgccca gcttcatggc ctggaccgcc acctgcgtgt
     1621 cggcggccgc ggtcaacatc accaccgcga tttccgggta ggacaggacc aggcgcttca
     1681 acaggtcgat gcccgtgagc ccgggcatca tgatgtcgct cagcaccagg tgaatgggct
     1741 cgcgctcgat cagccccagc gcgcgctccg cgccgttcgc ggtcttgcag cggtagccca
     1801 ggtgaatgag tttggtgctc aggagctcgc ggatgttcgg gtcgtcgtcg acgatgagaa
     1861 gcgtggtggg ttccatgccg tgtgtcgggt cacatcctcg tggtggtgtc ggaagcgcca
     1921 agatcgagcg gcgcgcgagc gggatcagat cccgataccg cagagtactc caatcccgag
     1981 gtctttgaca agcggacttt ttccggcacg ccgcgcgcgg cgcccgcgga cccggaactt
     2041 gacaagggcc ccgcggactc gatattctcc gcccgcaccg tgtgccgcgt ttgtgcgacg
     2101 cacggtgcga cccgccgttg agatggggct gtagcgcagt tgggagcgcg tcagactggc
     2161 agtctgaagg tcaggggttc gatccccctc agctccacca accctcgctc gcgaagggca
     2221 acgcagggcc tggtgtcccg gcccgaggct gccagatcag ttggttctcc gggttggtct
     2281 ttctccgatc ctcgctcgcg aagggcaacg cagcgcccgc cgtcccggcg cgaggctgcc
     2341 agatctttag gccttccgct cttttggttt tctcatcctc gctcgcgtag ggcaacgtag
     2401 cgcctgtatt cccggcgcga ggctgccaga tccgttgcac gtgttccccg gttcccatat
     2461 tccacgttct gtttcagcat gccgtttcgc cccctcgccg gttcacccat tcaccggctc
     2521 gtcctttgtc tctcaccgtg tcttgacccg ctagcccgca acgggctaag ctggctttat
     2581 gagccttgcc acaatcgatc aaaaactgtt ggagaaggtc acgaacacca tcgtcgagcg
     2641 atttcatcct cgccgcatca tcttatttgg aagccgcgcc cgcggcgagg cacgcccgga
     2701 cagcgatgtc gatctgttcg ttgaaatgga aagtacacgt cgccctcctg agcgggcgat
     2761 cgaagtcagt tcggtgttcg gtctgcgtcc ctggccattg gatgtcgtgg tatacacgcc
     2821 ggacgaggtc aaacgcctgc gccgcgtgag gggcacgctg ttgtccgtga tcgagcgtga
     2881 gggcaccgtg ctgtatgagt gcgcctgaat cgaacttcgc ggcgtgggtg cgaaaagccg
     2941 agcacgatct tctcaacatt caaaataatc tggcctccag cgacgtccca tgggacacgg
     3001 tgtgcttcca cgagatcgtg cagaagaagc ggggcatttc cgccgacacg gcgttgcgcc
     3061 tcggccggta tttcaccatg tccccggaat tttggatgaa cctgcaagtc cattacgacc
     3121 tggagtctga gcgggacaag ctcgctcaca agctggaaaa gacggtacgc gtgtacgcgg
     3181 cgtaaacatg gcacggcgga cacgagactg gaacaaggga ctggccaacg atctcaagga
     3241 caccaccttc atgcgggcgt tcatcaatgc gtcgcttgac gaaggcgttc ccgttcagca
     3301 ggtcattcgc aagcttgctg tgctgaaccg cggcgtgaga gccgacaagg cgtcagacgc
     3361 taaagggcac tgagcggcgc actaattgcg cacagcgctc ggggagaaca agatgcaggt
     3421 cacacgagca ttccgggtca ccatcatgga gcgggcgggc cgcgacgcgc ggtttcgcca
     3481 acacctgttg atcgaggcga tcaaccaatt gctgaccggt gatctgtccg cgggcaaggc
     3541 gatgctgcgt gactacatca atgcgacgat ttccttcgat caattggccc gcaagctcaa
     3601 gaagtccagc aagagcctgc accggatgct cggaccgcag gggaacccca ccgcggagaa
     3661 tctcttcgga atgatcaaag tgctccaggc gcatgaacgc gtgcaactcc acgtgaagtc
     3721 gcagcgatcc gccgcgtgac gtaggggaat gttgccgtga aaaagcactc gttggacgtc
     3781 gtccgcggga gtagcaatgt ctaccgcgat cgcggatttg caacatcaag tttgaccgtg
     3841 cagagatgac atcccgataa aaactgaggt ggtcgcaata tgcgaccacc tctagaagat
     3901 gagcagccat cgcccccaac ctgaaggagc ctggttatgg cgaataggaa acgggtcgtt
     3961 atcccaacgg agcgcatcga gggacagatt gttctggtcc ggggccagaa agtcatgctc
     4021 gacgccgatt tggcggtgct gtatgacgtg caagtgaagg cgttgaacca agcggtcagg
     4081 cgcaacatcg agcgattccc cgaggatttt atgttccaac tgaccgcgga agagattacg
     4141 cgtctaaggt cacaaactgt gaccttgaag accggagacc cagctcgtat gggtttcgat
     4201 gcaaacgcct caggtttgag gtcacaaatt gtgacctcaa acgcggacga ccggagc
//
LOCUS       DBNGTT010000518         4721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1018138, whole genome shotgun sequence.
ACCESSION   DBNGTT010000518 DBNGTT010000000
VERSION     DBNGTT010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1018138"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC16BC_12025"
     CDS             <1..596
                     /locus_tag="AC16BC_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HIR3242387.1"
                     /translation="IRLNNDVTMDLKLDVTSIGGLDPLAKQYSFGTRSTDTTINVRDG
                     ETVVLGGLIRDEDRKSVNKVPILGDIPILGKLFSSTETQKVKSDVVLTITPRVIRGLS
                     APEGGVQSFWSGTEEGYATQPLFTPTESVSQPSASEPGETVPMGGTMLAPSAPAPAPS
                     GSSTTGRPSSLNQPSRQGTPPVVFPVKPVVPAPSTAQ"
     gene            613..1131
                     /locus_tag="AC16BC_12030"
     CDS             613..1131
                     /locus_tag="AC16BC_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3242388.1"
                     /translation="MTEPRGESGVTFLELLATMAIIMILVSVAMPLSKITAKRTKEIE
                     LRQALREMRTAIDRFKDDWNRDGNNKIGPLCQTNQLSCIEVTSVNGYPKTLETLLRVD
                     MSGEAANIQGVTVKRYLRKLPTDPMTGKVEWTARCYTDDPDETSWCGEDVFDVHSTSE
                     ATGLDGTPYNEW"
     gene            1150..1575
                     /locus_tag="AC16BC_12035"
     CDS             1150..1575
                     /locus_tag="AC16BC_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3242389.1"
                     /translation="MFGVRFLRSLVMRSRVTTAWGPLGAAVLGAGIAVSAMPVPEVRA
                     EYGDIVMNREAEKAEMPPVVFPHWFHRIRFKCKVCHEDIFLMKAGANEVTMIKIIKGE
                     YCGKCHNGRIAWAPLYCDRCHSGKSQYSIAPSQGGGALP"
     gene            1637..2254
                     /locus_tag="AC16BC_12040"
     CDS             1637..2254
                     /locus_tag="AC16BC_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR3242390.1"
                     /translation="MWVIAPGLASAQSSAPPAGAAPSTANADQERLQRIKEKLKTVDP
                     SMVIYLDSRGNIAGTGDPGGVVGRAEIAGKGSHPPALELSGFPKDQYDLVDWAATMVQ
                     GKIKPRDAVDPSVPEVPPFDLDVVITTKSKFQPDVLFSHKVHTLWLSCDNCHEKIFKK
                     KAGGNPEMHMTKIAAGEFCGRCHDRVAFPLTDCLRCHVLAKDRAK"
     gene            2251..2619
                     /locus_tag="AC16BC_12045"
     CDS             2251..2619
                     /locus_tag="AC16BC_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3242391.1"
                     /translation="MTRWWWAAGALAVAVAAFLPSALSHGQEARIVDVVIAKDSYAFD
                     PPRVAIEVGDTVRWRNEDVRRHLTSSIPGFGPDTNLEIFCEDLYAGKSCVHTFTKPGR
                     YPYFCFVHNRMQGEVVVVDR"
     gene            2609..3295
                     /locus_tag="AC16BC_12050"
     CDS             2609..3295
                     /locus_tag="AC16BC_12050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3242392.1"
                     /translation="MTGSRPAFRRAHTALLVGALGFALVIPGCRSAPQSPKTPLEEAL
                     ESVTAPTEGETPVLPPGVTLGEAPAEGLSPEAVGVPSQDPAEAAVGLGIEPHPLAFAF
                     LPQTEQGYVDWVAAVDLGVIRPIDSLEPMRKVLEPMNFNVIFPVKGDLPDVVYPHYGH
                     TLWLDCKNCHPGIFVMRAGANRVTMEAIAKGEFCGRCHGKVAFSLADCNRCHSRPKPG
                     KPFIIPRASG"
     gene            3305..>4721
                     /locus_tag="AC16BC_12055"
     CDS             3305..>4721
                     /locus_tag="AC16BC_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3242393.1"
                     /translation="MVRWPGWVPILVGALAMLLYAESVAGPFQYDDWFTVVESDPSHA
                     QAGPALPGRPEAFRPVTTGSRVFDHAWALLLPTLAQDHPAFGFHLNNVILHGAASALV
                     WWVVRLWWADALMAALAGALFAIHPFHSEAVNYISARSSVLAACWELAALGAYTSWRR
                     GLGSRWWMVGVVSAAAALGAKESAVTLPLLLWLVDAVLIAPADGWRARVTRLWPWAVL
                     VAGYVAARLLAVSDVTGGSEYSSGDRLAALATGLWILGAAARDAWWPWSLSVEHGIEP
                     LHGARVWLVLVLAALAAIGAVVAWRRRRSSESPEGVLGMGLFALAWWVAAALPTLALP
                     FITHVALYLENRFYLASVGFIAFGAWLAARAWRSVASRAGPAVPIVAALVLVIGLVAG
                     SHARTTVWKSEVVLWRDAANKAPGSALAHAMLGAAYLGQNRPDLAIKPLEQAVRLDPR
                     YPLAATNLGAAYAGVDRWDDAI"
BASE COUNT          770 a         1468 c         1619 g          864 t
ORIGIN      
        1 gtatccgact gaataatgac gtcacgatgg atctgaagct tgacgtgacg tccatcggag
       61 gcttggatcc gctggcaaag cagtattcgt tcggcacgcg ctcgaccgac acgacaatca
      121 acgtgcgtga cggagagacc gtagtgcttg gcgggctgat tcgtgacgag gatcggaagt
      181 cggtcaacaa ggtgcccatc ttgggtgata tcccaatcct gggcaagctg ttttcatcca
      241 ccgagacgca gaaagtcaaa agtgacgtgg tcctaacgat cacgcctcgc gtgatccgag
      301 ggctgtccgc gccggaaggc ggcgtgcagt cgttctggtc cggcaccgag gagggctacg
      361 cgacgcagcc gttgttcacc ccgaccgaat ccgtcagcca accgtcggcg tccgagcccg
      421 gcgaaacggt gcccatgggc ggcaccatgc tcgccccctc cgcgccagcg cctgcgccat
      481 cgggttcgtc gacgaccggg cgtccgtcct cgttgaatca gccgtcccgt cagggaacgc
      541 cgccggtggt gttcccggtg aagcccgtgg tgccggcgcc gtcgaccgcg cagtagcgcg
      601 ggttccaggg cgatgaccga gccgcgcggc gagtcagggg tcaccttcct tgaactgctc
      661 gccaccatgg cgatcatcat gatcctcgtg tccgtggcca tgccgctctc gaaaatcacc
      721 gcgaagcgca ccaaggaaat cgagctccgc caggcgttgc gtgagatgcg caccgcgatc
      781 gatcggttca aggacgattg gaatcgcgac ggcaacaata agatcggtcc cttgtgccaa
      841 accaaccagt tgagttgtat tgaagtgacg agcgtcaacg gctatccgaa gaccctcgag
      901 accttgttgc gggtggacat gtccggagag gccgcgaaca tccaaggggt gaccgtgaag
      961 cgctacctgc gcaagctgcc gaccgatccc atgacgggca aggtcgagtg gacggcgcgc
     1021 tgctacaccg acgaccctga tgagacgagc tggtgcgggg aagatgtctt cgacgtgcat
     1081 tccacgagcg aggccacggg cctcgacggc acgccgtaca acgagtggtg acagccgggg
     1141 cgagttgaca tgttcggggt tcgttttcta cgctcattgg tgatgcgatc tcgggtgaca
     1201 acggcgtggg gcccgcttgg ggccgcggtg ctcggcgcgg ggatcgccgt gagcgcgatg
     1261 ccggttcctg aagtccgcgc tgaatacggc gatatcgtga tgaatcgcga agccgagaag
     1321 gccgagatgc cgccggtcgt cttcccgcac tggtttcacc ggatcaggtt caagtgcaag
     1381 gtgtgtcacg aagacatctt cctgatgaag gccggcgcca atgaggtgac gatgatcaag
     1441 atcatcaaag gcgagtactg cggcaagtgc cacaacggcc ggatcgcctg ggcgccgttg
     1501 tattgcgacc gttgtcattc cggaaaaagc caatactcca tcgcgccgtc ccaaggcggc
     1561 ggcgcccttc cgtagtacag tgctcctccg gcacggaatc cggcgaagaa tcctgggtct
     1621 cgcgcttttg ggggccgtgt gggtgatcgc gcccggcttg gcgtcggccc aatcctccgc
     1681 cccgccggcg ggagcggccc cgtcgacagc taatgccgac caagaacgtc tgcaacggat
     1741 caaagagaaa ctgaagaccg tggacccctc gatggtcatt tatctggaca gtcgcgggaa
     1801 catcgccggg accggtgacc ccggaggagt ggtgggccgg gcggagatcg cgggaaaggg
     1861 gagtcatccg ccggccttgg aattgtccgg gtttccgaag gatcagtatg atctggtgga
     1921 ctgggccgcg accatggtcc agggcaagat caaaccccgc gacgccgtgg acccgagcgt
     1981 gcccgaggtg ccgccgttcg atctggacgt ggtcatcacc accaagagca agttccagcc
     2041 ggacgtcctg ttttcccaca aggtccatac cttgtggctg tcgtgcgaca attgccatga
     2101 aaagatcttc aagaagaaag ctggaggaaa cccggaaatg cacatgacga aaattgccgc
     2161 gggcgaattt tgcggacggt gccacgatcg ggtcgcgttt ccgctgaccg attgcctgcg
     2221 gtgccacgtg ctcgccaagg atcgcgccaa atgacccgct ggtggtgggc ggcgggcgcg
     2281 ctcgccgtgg cggtggccgc gttcctgccg tcggcgcttt cgcacggcca ggaggcccgg
     2341 atcgtcgacg tggtgatcgc caaggacagt tacgccttcg acccgccgcg ggtcgcgatc
     2401 gaagtgggcg acaccgtgcg gtggagaaac gaagacgtcc ggcgtcattt gacgtcgagt
     2461 atcccggggt ttggtccgga cacgaacctg gagattttct gcgaagacct gtatgccgga
     2521 aaatcgtgcg tccacacctt cacgaaaccc ggtcgctacc cctacttctg tttcgtacac
     2581 aaccggatgc agggcgaggt cgtggtcgtt gaccggtagt cgtcccgcgt ttcgacgcgc
     2641 gcacacggcc ctcctggtcg gtgcgctcgg cttcgcgctc gtcatcccag gctgtcggag
     2701 cgcgccgcag tccccgaaga cgccgctcga agaggcgctc gagtccgtga ccgcgccgac
     2761 cgagggggaa acgccggtct tgcctcccgg cgtgacgttg ggagaggcgc cggccgaagg
     2821 cctgtcgccg gaggcggtgg gcgtgccgtc tcaagatcct gccgaggccg cggtggggct
     2881 gggaatcgaa ccgcatccgt tggcgttcgc cttcctgccc caaaccgagc aagggtacgt
     2941 ggactgggtg gccgccgttg acctgggcgt gatccggccg atcgactcgc tcgagcccat
     3001 gaggaaggtg ctcgaaccca tgaacttcaa cgtgattttc ccggtcaagg gggatttgcc
     3061 cgacgtggtc tacccccatt acggccacac gttgtggctc gactgcaaaa actgccatcc
     3121 cggcatcttc gtcatgcgcg ccggcgccaa ccgcgtgacc atggaggcga tcgcgaaagg
     3181 cgagttttgc gggcgctgcc acggaaaggt cgcgttctcg ttggccgact gcaaccgctg
     3241 ccactcgcgt cccaagccgg gaaaaccctt catcatcccc cgcgcgagcg ggtaggcgct
     3301 ccccatggtg cggtggcccg ggtgggtccc catcctcgtc ggggcgctcg cgatgctgct
     3361 gtacgccgag agcgtcgccg gcccgtttca gtacgacgat tggttcaccg tggtcgagtc
     3421 ggatccgagc cacgcccaag cgggcccggc gctcccgggt cgacccgaag cgtttcgccc
     3481 ggtcacgacc ggctcgcggg tgttcgacca cgcgtgggcg ctcctgctcc cgaccctggc
     3541 gcaagaccat ccggcgttcg ggtttcatct gaacaacgtc atcctgcacg gcgcggcgtc
     3601 cgcgttggtc tggtgggtgg tcagattatg gtgggcggac gccttgatgg ccgcgctggc
     3661 cggcgcgctc tttgcgatcc atccgtttca ttccgaagcc gtcaactaca tcagtgcccg
     3721 gtcctcggtg ctggccgcgt gctgggaact cgcggccctg ggcgcgtata cgagttggcg
     3781 ccggggcctg ggatcccggt ggtggatggt cggggtcgtg agcgcggcgg ccgcgctggg
     3841 cgcaaaagag tccgcggtca cgttgccgct gctgttgtgg ctggtggatg cggtcctgat
     3901 cgcgccggcc gacggttgga gagcccgcgt gacgcggctg tggccgtggg ctgtccttgt
     3961 cgccggatac gtcgcggctc ggctgttggc ggtgtcggac gtgaccggcg gctccgagta
     4021 ttcctcgggt gaccggctgg cggcgctggc gaccgggttg tggattctgg gcgcggctgc
     4081 gcgggatgcg tggtggccgt ggtcgttgag cgtcgaacac ggcatcgaac cgcttcacgg
     4141 ggccagggtg tggctggtgt tggtgcttgc cgccctcgcg gccatcggcg ccgtcgtcgc
     4201 gtggcgacgg cgccggtcgt cggaatcgcc ggaaggagtt ctcggcatgg ggttgttcgc
     4261 gctcgcgtgg tgggtggcgg ccgcgctccc cacgctcgcg ttgccgttta tcacccacgt
     4321 ggcgctctac ctcgagaacc ggttttacct ggcgagcgtc ggcttcatcg cgttcggcgc
     4381 ttggttggcg gcccgcgcgt ggcgatccgt tgcgtcccgc gccggccccg cggttcccat
     4441 tgtcgcggca ctcgtactgg tcatcggtct ggtcgcggga agtcacgcgc ggaccacggt
     4501 gtggaagtcc gaagtggtcc tgtggcgcga cgccgcgaac aaggctcccg gcagcgcgtt
     4561 ggcgcacgcc atgctcggcg cggcatatct cggtcaaaat cggccggacc tcgcgatcaa
     4621 gccgttggaa caggcggtcc gactcgatcc ccgctatcca ttggcggcga cgaatctggg
     4681 cgccgcctac gcgggggtcg atcggtggga cgacgccatc c
//
LOCUS       DBNGTT010000519         3182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_3317594, whole genome shotgun sequence.
ACCESSION   DBNGTT010000519 DBNGTT010000000
VERSION     DBNGTT010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_3317594"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>3182
                     /locus_tag="AC16BC_12060"
     CDS             12..>3182
                     /locus_tag="AC16BC_12060"
                     /inference="COORDINATES: protein motif:HMM:NF019325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HIR3242394.1"
                     /translation="MGGTVVNADGTVRCARDTLSGSGLGQFGNWYAPVVADLTGDGHP
                     EIVAGNTAYRADCTILWRVSGVPDGINAVANFDDDPAPEIVLVTSGFVSGQPAGGKVY
                     LLESDGTMRWGPIALRGLEPTAQSAGVGGPPVVADFDGDGRADIGVSGFDRYFVLDRD
                     GAVKTSFFIPYNQGFGDGFYAAPTVFDLNGDGRPEVLFNNNGYFRVFDGTTGALLFQE
                     RFGAAFNSYQNVLIADVDGDQHAEAVVFGYGWYFSGDGLRVYGSATNNWVNARKVWNQ
                     PDYHVTNVNDDGTIPQYEAPSWLVNNTYRVQTPVGEVTNPYLAPNLTASYLRAVQTSA
                     GVTLTVRIGNGGAVAAASNAPVSLYDGDPAAGGALIGTGQTTRALAPGDYQDVVFTWT
                     GASVGTRTIVAVVDSNATRTDCLKDDNRASLSTTIVPILPDLSVGGNDLQVLGPLSEG
                     RLVPVRVTVHNTGGADAPTSSVRLTLGDPTQGGVELARTTLPGVAAGASASVDLVWDS
                     LGATGTNYLYAQVDPDHTISEASTANNTALTAVDLAAPAQPDLSITQVGVSSSTPHEG
                     QSITVTAQVGNRGADVGGAGVALYLGDPNAGGVSLGAQTIPTILAHGQSVTVTWSVDT
                     LGRSGSHELVAVADPGGTIPEIDETNNRGTATVTILHSGLGAAIATDQPSYTANEAAT
                     ATVTLYNSGSARTVDVEVITEDTAGVWVQTVATQAGLALGDHETRIISGLAFNTGTTF
                     AGTYRLRVRAREAGSVVAEATSGFAILPVVQAETRLVADKQAYSSHETVTLTSTVTSQ
                     SPNHVLTGVTATITLIDPASAVVFTETRALMDLLPDARFELKSFWDTGVQVAGLYTAS
                     VSVQGADGLSTTSQTAFEILSSAEQAAALAGSIAVEPRTIIETEHANIAYTIQNIGNE
                     IDLPLIQVEVLVVDPDTEQAVRTLTGEASLNGREVYETAIAFDSAGLAPKSYLFVLRG
                     ATAGVTQALGSAGLTIQPSPNTAPVADAGPDQIGYAGQAIAFDGTASHDPNNDPLTFA
                     WRFVSVPTGSQLTTTALANATSPTPSF"
BASE COUNT          555 a         1002 c         1106 g          519 t
ORIGIN      
        1 ccgagatcgt catgggcggc accgtcgtca acgccgacgg cacggtgcgc tgtgcccgag
       61 acacgttgag cggcagcggc ctgggccaat tcggcaactg gtacgcgcca gtggtggcgg
      121 atctgaccgg cgacgggcat ccagagattg tggcggggaa cacggcctat cgggctgatt
      181 gcacgatcct gtggcgggtg tcgggcgtgc ccgatgggat caacgcggtc gccaatttcg
      241 acgacgatcc cgcgcccgaa atcgtgctgg tgacgtctgg gtttgtgtcc gggcaaccgg
      301 ccgggggcaa ggtgtatctc ttggagtctg acgggaccat gcgctggggg ccaatcgcgc
      361 tgcgaggcct ggaacccacg gctcagagcg cgggggtggg tgggcctccg gtggtggccg
      421 attttgacgg agatggacgc gcggacatcg gcgtcagcgg gttcgatcgg tattttgtgc
      481 tggatcggga cggggcggtc aagaccagtt tcttcattcc gtacaaccag gggtttggcg
      541 acgggttcta cgcggccccc acggtctttg atctgaatgg agacggacgc ccggaagtgt
      601 tgttcaacaa caacggctac ttccgggtct ttgacggcac gaccggagcg ttgctctttc
      661 aagagcggtt cggggcggcg ttcaacagct atcaaaacgt gctgatagcc gatgtcgatg
      721 gggaccagca cgccgaagcg gtcgtgtttg ggtatggctg gtacttcagc ggtgacgggc
      781 tgcgagtcta cggttccgcg actaataact gggtcaatgc gcgcaaagtg tggaaccagc
      841 ctgactatca cgtcaccaac gtcaacgacg acggcaccat cccccagtac gaagcgccga
      901 gttggctggt gaacaacacc taccgcgtgc aaacgccggt cggcgaagtc acgaatcctt
      961 acttggcgcc gaacctgacc gcctcgtacc tgcgcgcggt gcagaccagc gcgggcgtga
     1021 ccctcacggt gcgtatcggg aacggtgggg cggtcgcggc cgcatcgaac gcgccggttt
     1081 ccctctacga cggcgatccc gcagccggtg gcgcgttgat cggcaccggg cagaccacgc
     1141 gcgcgttggc accaggggac tatcaagacg tggtcttcac ttggaccggg gcgagcgtgg
     1201 gcacgcgcac gatcgtggcc gtggtggatt ccaacgccac gcggacggac tgtctcaagg
     1261 acgacaaccg cgcgagcctg agcacgacca tcgtgcccat tctgcctgac ctgagcgtgg
     1321 gagggaacga cctccaggta ctcggcccgc tgtcagaagg ccgtctcgtc ccggtgcgcg
     1381 tgaccgtgca caataccggc ggggctgatg cgccgacctc ttccgtgcgt ctgaccctgg
     1441 gcgatcccac gcagggcggg gtggagctgg ctcggaccac gcttccagga gtggccgccg
     1501 gagcgagcgc ctcggtggac ctggtctggg acagcttggg ggcgaccggc accaattatc
     1561 tctacgcgca ggtggatccg gatcacacga tcagcgaagc gtccacggcc aacaacaccg
     1621 cgctcacggc ggtcgacttg gccgcgccgg ctcagccgga tttgagcatc acgcaggtcg
     1681 gcgtgtcgtc gtccaccccg cacgagggac aatcgattac ggtgacggcg caggtgggca
     1741 accggggcgc ggacgtgggc ggggcaggcg tggccttgta cctgggcgat ccgaacgccg
     1801 gtggcgtcag cttgggcgcg caaaccattc cgactatttt ggcccatggc cagagcgtga
     1861 ccgtgacgtg gtcggtcgac accctggggc ggtccgggag ccacgagctg gtggccgttg
     1921 ctgatccggg cggcacgatt ccggagatcg atgagaccaa caaccggggc acggcgaccg
     1981 tcacgatctt gcacagcggc ctgggcgctg cgatcgccac cgatcagccg tcctacaccg
     2041 ccaacgaggc ggcgaccgcg acggtgacgt tgtacaactc cggctccgca cgcaccgtgg
     2101 acgtggaagt catcacggaa gacacggccg gcgtgtgggt gcagaccgta gccactcagg
     2161 cgggcttggc gctgggcgac cacgaaacgc ggatcatctc cggcctggcg ttcaacaccg
     2221 gcactacgtt cgcgggcacg taccggcttc gcgtgcgcgc gcgcgaagcc ggctcagtgg
     2281 tggccgaggc cacgagcggc tttgccattc tcccggtggt gcaggccgag acgcgcctgg
     2341 tggcggacaa gcaagcgtac tcctcgcacg aaaccgtgac gttgacgagc acggtcacga
     2401 gccagagccc caaccacgtc ctgaccggcg tgaccgcgac catcacactg atcgatcccg
     2461 cgagcgcggt tgtgttcacg gagacgcgcg cgctgatgga tctgttgccc gacgctcgat
     2521 tcgagctcaa gagcttctgg gacacgggcg tgcaggtggc ggggctctat accgcgtcgg
     2581 tgagcgtgca gggcgcggac ggcctgtcca cgacgagcca gaccgcgttc gagatcttgt
     2641 cgagcgcgga acaggccgcg gccttggcgg ggtccattgc ggtcgagccg cgaacgatta
     2701 ttgaaaccga gcatgccaat attgcctata cgatccagaa catcggcaac gaaatcgacc
     2761 tgcccttgat tcaggtcgag gtgttggtgg tggatccgga cacggagcag gccgtgcgca
     2821 cgctgaccgg cgaggcctcg ctcaacggcc gcgaggtcta cgagaccgcg atcgcgttcg
     2881 acagcgcggg cctggcgccc aaatcgtatt tgttcgtgct gcgcggcgcc accgcgggcg
     2941 tgacccaggc gttggggagc gcgggcctga cgatccagcc cagccccaac accgcgccgg
     3001 tggccgacgc ggggccggat cagatcggat acgcgggaca agccatcgcg ttcgacggga
     3061 ccgcgagcca cgatccgaac aacgatcccc tgacatttgc ctggcggttc gtgtccgtgc
     3121 ccacgggcag ccagctcacc accacggccc tggccaacgc cacgtcgccg acgccgtcgt
     3181 tc
//
LOCUS       DBNGTT010000520         6371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1018490, whole genome shotgun sequence.
ACCESSION   DBNGTT010000520 DBNGTT010000000
VERSION     DBNGTT010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1018490"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="AC16BC_12065"
     CDS             complement(<1..1732)
                     /locus_tag="AC16BC_12065"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR3242395.1"
                     /translation="MATKTSKIIYTHVDEAPMLATYSLLPIVNAFTKAAGVAVEERDI
                     SLSGRIIASFPEYLTAAQKQADDLAELGELAKTPDANIIKLPNISASIPQLKAAIKEL
                     QSQGYKLPEYPEDPKTDKEKDIKARYDKVKGSAVNPVLREGNSDRRAPLSVKQYARKH
                     PHKMGPWTADSKTHVSTMTKGDFRSNEKSVTLTEATDARIEFVDQSGKATVLKPKLAL
                     KAGEVIDATYMSRRALREFIEAQIEDAKKQGVLFSIHLKATMMKVSDPKIFGHAVSVY
                     FKDVFEKYTATFDKLGVDPDNGLGDLYAKIKALPEAERAAIEADIKAVYAKRPPMAMV
                     NSDKGITNLHVPSDIIIDASMPPVIRDSGKMWNPEGKLQDAKCVIPDSSYAPVYREVV
                     EFCKQHGAFDPRTMGSVPNVGLMAQAAEEYGSHDKTFKAPGNGTIRVAAASGQTLLEH
                     QVEEGDIWRMCQVKDAAIQDWVKLAVTRARATGAPAVFWLDKTRAHDAELIKKVNAYL
                     PKHDTTGLDIRILSPAEATRLSLERMKEGKDTISVTGNVLRDYLTDLFPILEIGTSAK
                     MLSIVPLLNGG"
     gene            complement(2408..2959)
                     /locus_tag="AC16BC_12070"
     CDS             complement(2408..2959)
                     /locus_tag="AC16BC_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242396.1"
                     /translation="MYSRTARVGILLVATWCSAAYGNESVRAGDSFPQPKALSAHRSV
                     VATVTKVVSGSVLLRTEDGTLRNVAVTDAARDGMSRLRPGDEVDLILDYGNSVVAIAP
                     PRGTGAYVGDEVTGTVQHGAPPFFDRFNKRITLKTTDGEVQTFELRDAAATKLNGTPQ
                     GRMIVLVMDGHHRAFDAYRPLTR"
     gene            complement(3030..5189)
                     /locus_tag="AC16BC_12075"
     CDS             complement(3030..5189)
                     /locus_tag="AC16BC_12075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3242397.1"
                     /translation="MTSPSRYEFQQFPPASSRGARWFAAGIALVAALAPASVRAQVVE
                     PRADGYFQEQVGARIQALGRFDLQRTTALHWQWSEVMQTALAFDAGRDARLQETLGQS
                     IAAAARALEVDRAALAAALAESLGTQRRVRSGAWYQEQIGKGIVQTAAEVPPHDPRFG
                     ERLRSKVVVLSAMERRVGRQAEERLAALRAREAQFPRDALARLAASVTDGYRVAHWYD
                     VSHDAWAALALTDIRNGLALLRKAEDYRRLADAEIAALRSRWGVGGFWEFGAASLAGI
                     IVAMVWVGATAPREVPGSGARAESTVRMPLPYQWRREVAMGQSTTLFSWPREVIFGGE
                     CSRDIGRYAARDHVKHAMILTDPGVVQKGLLEPVKASLAEAGVGCEVIDQVPREVPHT
                     AVAEIAARCQQARTELLVAVGGGSVIDTAKAVGILLTNGGAIQDYEGVDRVGRPITLL
                     YVVPTTAGGGSDTSQFCIVLDTARKKKIEIFSRKLLPERIFIDPIMTRSMPPDLTASS
                     GMEALGNAIEAYCSTWASPLTDSLALDAIRLISENLRAAVANGRHMEARTCMALAAFE
                     AGLAFTNAQSGAVHALGHSLAGRFDIPERIGDAILLPHVMKANLNADMPRMAKVAETM
                     GEPVAGLSVRAAAQQAIEAVKQLLVDVGLPTSLETVGADKNAISAMSEQAFQDTFMRT
                     NPRALKREDIEAIFEEAFVAYNDEDFVPGRGARVTTH"
     gene            5594..6250
                     /locus_tag="AC16BC_12080"
     CDS             5594..6250
                     /locus_tag="AC16BC_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3242398.1"
                     /translation="MPRIPRGQVPGLAYHVLNRGNGRATIFHKDADYRAFCDLLIAAR
                     KKFPVAVFGFCVLPNHFHLVLQPETPAALSAFMQWWLTSHVRRYHRHYHSSGHVWQGR
                     FKSFPIQSDEHLLTVLRYVLLNPVRADLIPRAALWPWSSLNPAYPPEPWPLEPPADWT
                     VRSLATPLPDEDLTQIRTCVNRQAPYGAPNWVSQTARTLGLQSTLRARGRPPKPILEK
                     "
BASE COUNT         1002 a         2167 c         2045 g         1157 t
ORIGIN      
        1 cgccgccgtt cagcaacggg acgatcgaga gcatcttggc gctggtcccg atttccagaa
       61 tcgggaacaa atccgtgaga taatcgcgca gcacgttgcc ggtcacggag atcgtgtcct
      121 tgccctcctt catccgctcc agggacaagc gcgtggcctc ggccggggac aggatccgga
      181 tgtccaggcc cgtggtgtcg tgtttgggca gataggcgtt gaccttcttg atcagctccg
      241 cgtcgtgcgc cctggtcttg tccagccaga acaccgcggg cgcgcccgtg gctcgagcgc
      301 gggtcacggc cagcttgacc caatcctgga tcgccgcgtc cttgacctgg cacatgcgcc
      361 agatgtcgcc ttcttccacc tggtgttcca gcagggtctg gcccgacgcg gccgccacgc
      421 ggatcgtgcc gttgcccggc gccttgaagg tcttgtcgtg cgagccgtac tcctccgcgg
      481 cctgcgccat caggcccacg ttgggcacgc tgcccatggt gcgcggatca aacgcgccgt
      541 gttgcttgca gaactccacc acttcgcgat agaccggcgc gtagctgctg tccgggatca
      601 cgcacttggc gtcctgcagc ttgccctcgg gattccacat cttgcccgag tcgcggatca
      661 ccggcggcat ggacgcgtcg atgatgatgt cgctgggcac gtgcaggttg gtgatgccct
      721 tgtccgagtt caccatggcc atgggcggcc gcttcgcgta cacggctttg atgtccgcct
      781 cgatcgcggc ccgctcggcc tcgggcaagg ccttgatctt ggcgtacagg tcgcccaagc
      841 cgttgtccgg gtccacgccc agcttgtcga acgtggccgt gtacttctcg aacacgtcct
      901 tgaagtacac ggacaccgcg tgtccgaaga tcttggggtc cgagaccttc atcatggtgg
      961 ccttgaggtg gatcgagaac aacacgccct gcttcttggc gtcttcgatc tgcgcttcga
     1021 tgaactcgcg cagcgcgcgg cggctcatgt acgtggcgtc gatgacttcc ccggccttca
     1081 acgccagctt gggcttcaac accgtagcct tgccgctttg atcgacgaat tcgatgcgcg
     1141 cgtccgtggc ctcggtgagc gtgaccgatt tctcgttgga ccggaaatcc cctttcgtca
     1201 tggtcgagac gtgggtcttg gagtccgcgg tccacgggcc catcttgtgc gggtgcttcc
     1261 tggcgtattg cttgaccgag agcggggccc ggcgatccga gttgccttcg cgcaacaccg
     1321 ggttcaccgc gctgcccttg accttgtcgt aacgcgcctt gatatccttc tctttgtcgg
     1381 tcttggggtc ttccggatac tcgggcaatt tatagccctg cgactgcaac tctttgatgg
     1441 cggccttgag ctgcgggatc gacgcgctga tgttgggcag tttgatgatg ttggcgtccg
     1501 gggttttggc cagttcgccc agctcggcca gatcgtccgc ctgcttctgg gcggcggtga
     1561 ggtattccgg gaaactggcg atgatccggc ccgacagcga gatgtcgcgc tcctccaccg
     1621 ccacgcccgc ggccttggta aaggcgttga cgatcgggag caaggaatag gtggccagca
     1681 tcggggcttc atcaacgtgc gtatagataa tcttcgaagt cttcgtcgcc atagcgcgtc
     1741 ctcacctggt gttggatttt gagagagcag gacaaccgac aacagagcca tcgttctaat
     1801 gcatttttcg ttgtagtgtc aaggacccgg aggacccaga acgcggggac aaacgaccca
     1861 ttccgtcaga gcgccggctt accggctcag agccctggaa ccctgcctcg aacggcgtgg
     1921 catggcccct cgggactcct gcggatgacg ttgcacttga cgttgacatt cctcgcgagc
     1981 cgggagtacc gtctgtcgga ccgcggtggg cgacgcccgt ctggcctggg caacacgatg
     2041 agattggggg ctgcaactcc ggactatgaa cgggccggct ccgcggcacc cacctagaca
     2101 aaagagcctc tggaccagcg cgtcagccca ccgcggtccc caatccgcgc ctcatggtcc
     2161 gcgcgaggag aaacgaaccg cgctacttcc ggatgtagcg ggcaagccgc gaccccacga
     2221 agagcagcct gccccccttt tccttgcccg aagggacgaa gagagaaaca atccaagtac
     2281 ggaagtaaca caagattccc agcgggtgtt tggttcgcgc cgggttgctg ggggggcacg
     2341 cgggccggcc gccccgggcc gggtgctcgg tccggggcgg cgcgtccgcg cgaaggagcg
     2401 gggtgggtta ccgggtgagc gggcgatacg cgtcgaacgc gcggtggtgc ccgtccatca
     2461 ccaggacgat catccgtcct tggggagtcc cgttcaactt cgtggccgcc gcgtcccgca
     2521 attcgaaggt ttgcacctcg ccgtcggtgg tcttgagcgt gatccgtttg ttgaagcgat
     2581 cgaaaaacgg cggggcgccg tgttggaccg tgcccgtgac ctcgtcgccg acgtacgcgc
     2641 cggtgccgcg cggcggggcg atggcgacca cggaatttcc gtaatccagg atcaggtcca
     2701 cctcgtctcc cggccggagc ctggacatgc cgtcccgcgc ggcgtccgtg accgccacgt
     2761 tgcgcaacgt cccgtcctct gtccgcagca gcaccgaccc gctcaccacc ttggtcacgg
     2821 tggcgaccac gcttcgatgc gccgagagcg ccttcggctg cgggaaagaa tccccggccc
     2881 ggacggactc gttgccgtag gccgcggaac accatgttgc gacgagcaaa atgcctaccc
     2941 gtgctgtacg cgaatacatg tgaccctcct tgtctccgtc cccgtgcgag cgtgtgtcct
     3001 tcgtccggga acgtcttgcc ccgtgaggcc tagtgggtcg tgacgcgcgc gccgcgaccc
     3061 ggaacgaagt cctcgtcgtt gtacgccacg aacgcctcct cgaagatcgc ctcgatgtcc
     3121 tcgcgcttga gcgcgcgcgg gttggtgcgc atgaacgtgt cctggaacgc ctgttcgctc
     3181 atggcggaga tcgcgttctt gtccgcgccc acggtctcga ggctggtggg caggcccacg
     3241 tccaccagca gctgcttgac cgcctcgatg gcctgctggg ccgcggcgcg aacgctcagg
     3301 cccgcgaccg gctcgcccat ggtctcggcc accttggcca tgcgcggcat gtccgcgttc
     3361 aggttggcct tcatcacgtg cgggagcagg atcgcgtcgc cgatccgttc cgggatgtcg
     3421 aaccggccgg ccaatgaatg gccgagcgcg tgcacggccc cgctctgcgc attggtgaag
     3481 gccagaccgg cctcgaacgc ggcgagcgcc atgcaggtgc gggcctccat gtggcgcccg
     3541 ttggccaccg ccgcgcgcag gttctccgag atcaggcgga tggcgtcgag cgcgagcgag
     3601 tcggtcagcg ggctcgccca cgtcgagcag taggcctcga tggcgttgcc cagcgcttcc
     3661 atcccgctgc tcgcggtgag gtcgggcggc atggagcggg tcatgatcgg atcgatgaag
     3721 atccgctccg ggagcagttt gcggctgaaa atttcgatct tcttcttgcg ggcggtatcg
     3781 agcacgatgc aaaactggct cgtgtcgctg cccccgcccg cggtggtggg gaccacgtac
     3841 aacagcgtga tcggacggcc cacgcgatcg acgccctcgt agtcctggat ggcgccgccg
     3901 ttggtgagca ggatgccgac cgctttcgcg gtgtcgatga cgcttccccc gccgaccgcg
     3961 acgagcaact cggtccgggc ctgctggcag cgggcggcga tctccgcgac cgcggtgtgg
     4021 ggcacctcgc gcgggacctg gtcgatcacc tcgcacccga cgccggcctc ggccagcgac
     4081 gccttcacgg gctccaggag ccccttctgg accacgcccg gatccgtcag gatcatcgcg
     4141 tgcttgacgt ggtcgcgggc cgcgtaccgc ccgatgtccc ggctgcactc gccgccgaaa
     4201 atcacttcgc ggggccaact gaacaatgtg gtggactgtc ccatggcaac ctctcttctc
     4261 cattgatacg gcaacggcat tctcacggtg gattccgcgc gcgcgccgga ccccggcacc
     4321 tctctgggcg cggtggcgcc gacccagacc atcgcgacga tgatcccggc cagcgacgcg
     4381 gcgccgaatt cccaaaagcc gcccacgccc catcgcgatc tgagggcggc gatctccgcg
     4441 tcggcgagcc ggcgatagtc ctcggccttg cgcaagagcg ccaacccgtt gcggatgtcg
     4501 gtcagcgcca gcgccgccca cgcgtcgtgc gacacgtcgt accaatgcgc cacgcgatac
     4561 ccgtccgtga cggacgcggc caggcgggcg agggcgtcgc gcgggaattg cgcctcgcgc
     4621 gcgcgcagcg cggcgagacg ttcctccgcc tgacggccca cgcgccgttc catcgcggac
     4681 agcaccacga ccttggatcg caggcgctcc ccgaatcgcg gatcatgcgg cggaacctcg
     4741 gcggccgtct ggacgatccc cttcccgatc tgttcctggt accaggcgcc gctgcggacg
     4801 cggcgctgcg tgcccagact ctcggccagc gcggcggcaa gcgccgcgcg gtcgacctcg
     4861 agcgcgcggg cggccgcggc gatggactgc cccagggttt cctggagccg cgcgtcgcgg
     4921 ccggcgtcga acgcgagggc cgtctgcatc acctcgctcc attgccaatg cagcgcggtc
     4981 gttcgctgca gatcgaaccg tccgagggcc tgaattctgg cgccgacctg ttcctggaaa
     5041 tacccgtccg cgcgcggttc gacgacctgc gcgcggacgc ttgccggggc cagcgccgcg
     5101 accagggcga tccctgccgc gaaccatcgc gcgccccgtg atgaggcggg tgggaactgc
     5161 tggaactcgt atcgagacgg actggtcatc gcacacgctc ctttgcaatc accgcgactg
     5221 aggaaaaggc cgtgggcgag gcggggcgcg cgccgcgcaa tggatccagc gcggccgcgt
     5281 tccgggaaca tgggatggtc tgctgcgtgt ctccatcaga gcctccttgc cggccctgac
     5341 ccgaaccgct tgcaggcgtc ggtccacctg cggtgggacg agaagcgtcc cgacaacgga
     5401 agaactgcga agaaatgacc gaagactctg gtcgtatacc accggctccg aaccagagtc
     5461 aagctggcgc ccaatcgcga ccgcaggcga acctgcgccc gggctcgggt tcgcgattca
     5521 ggaaatgagc tagaaaaggg gacaggctac ttttccttgc caggacatcc cgtcctgggg
     5581 tacgatcggc gccatgcctc gcatcccccg cggccaggtg cccggcctgg cctatcacgt
     5641 cctcaaccgt gggaacgggc gcgcgacgat ttttcacaag gacgccgact atcgcgcgtt
     5701 ctgcgatcta ctgatcgctg ccaggaagaa attcccggtg gccgtgttcg gcttctgcgt
     5761 cctgcccaac cacttccacc tcgtgctcca acccgagacg ccagccgcgc tcagcgcctt
     5821 catgcagtgg tggctcacca gtcacgtgcg ccgctatcat cgccattacc atagtagcgg
     5881 acatgtgtgg cagggtcggt tcaagagttt tccgattcaa tccgacgagc acctgttgac
     5941 cgttctccgc tacgtgctcc tcaatcccgt tcgggcggac ctcatcccgc gcgctgcgct
     6001 ctggccgtgg agcagcctca atccggcgta tccgccagag ccgtggccgc ttgagccacc
     6061 cgcagactgg acggtccgct cgctcgccac cccgctgccc gacgaggacc tcacccagat
     6121 ccgcacctgc gtcaatcgtc aagccccgta cggagcaccc aactgggtga gccagactgc
     6181 ccggacgctc ggcctgcagt ccaccctccg ggcgcgcggc cgcccgccca aaccgatcct
     6241 agaaaagtag cctgtcccct ggtatcttgg ttttacttcc ggtcataatg cgccggatgg
     6301 atcgtcgcga cgtcacagac cgctacccct gaggtccacg agaccgaaga aaagcctggg
     6361 tcgtgacgtc g
//
LOCUS       DBNGTT010000521         5332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_252447, whole genome shotgun sequence.
ACCESSION   DBNGTT010000521 DBNGTT010000000
VERSION     DBNGTT010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_252447"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="AC16BC_12085"
     CDS             <1..679
                     /locus_tag="AC16BC_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242399.1"
                     /translation="CIANYQGDPEVFLTMHGERLVGLIKDERLPLDEEEVRSTLQASI
                     KYGKDDLTIVDWDGAILFDPHGDFDANIELIEIANFQLLRTRMADQALDARLAQIAQM
                     LRTTPKQSLFRRREVRAMLREILRIRTETLLESPATEHHIKMIGDWYAARVYGLLARK
                     FHIEVWQTSIQHKLDTIEDVSAMVAENFSVSFHTTLDFILIAGWFLLLAGWFAYLFVD
                     VYLLSTR"
     gene            763..1866
                     /locus_tag="AC16BC_12090"
     CDS             763..1866
                     /locus_tag="AC16BC_12090"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3242400.1"
                     /translation="MPNSTDNAPAPVIELAGVTKRYQPDHAPAIDALSLTVARGHTLA
                     VLGPSGCGKTTLLRLIAGFEDPSSGMVRLGGHCVAGAGCWVPPERRGVGMVFQDYALF
                     PHLTVSRNISFGLHQMDRSARASRVSEVLELVGLAGLEDRYPHQLSGGQQQRVALARA
                     LAPKPIVVLLDEPFSNLDPDMRSRMRQEVDAILRQSGATTVLVTHDHEEAFAMADRVA
                     VLNRGRLEQLGTPEVVYHLPTTPFVAEFVGQADFVPGAVEGLQVRTELGPFPNTSGFP
                     TGMAVSVMIRPDDVLLVPSDDAPAIVVNRQFRGSENLYTVHLASGRPLHSTQPSDVVY
                     ELGSRVEIKINATHTVLFDPSVLPKHSPSHHEV"
     gene            1943..2017
                     /locus_tag="AC16BC_12095"
     tRNA            1943..2017
                     /locus_tag="AC16BC_12095"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1975..1977,aa:Val,seq:cac)
     gene            complement(2162..2545)
                     /locus_tag="AC16BC_12100"
     CDS             complement(2162..2545)
                     /locus_tag="AC16BC_12100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3242401.1"
                     /translation="MENYANRREFTRVKISVAVELTPPGQPKISGRGRDLSVKGVYVS
                     GPRQLPASTECPITIFLTGGGEPMSVRVLGRIVYADKEGMGIEFSEMDTDSFIHLQNL
                     VLRNSLDPAKAEREFQEHSGIKRKS"
     gene            2706..3347
                     /locus_tag="AC16BC_12105"
     CDS             2706..3347
                     /locus_tag="AC16BC_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR3242402.1"
                     /translation="MKHATDRAIPDSTLQDAPIVRLTGQAIAKVASALAGTANIGVRL
                     TVAREKGGFTYKFDVVAPGQVDPRDPTVPCGAWTFYLNHATVEWIRGAEIDYMSSGFT
                     QGWVIDNPNPAWDSELARRISEVFDKTINPGLAQHGGRVRLVDLQGTVAYVEMSGGCQ
                     GCSMATKTLRDGIMRVLAHEFPELTGVVDTTDHAGGSSPYFKGDQQGASPTLG"
     gene            3376..4110
                     /gene="pyrF"
                     /locus_tag="AC16BC_12110"
     CDS             3376..4110
                     /gene="pyrF"
                     /locus_tag="AC16BC_12110"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR3242403.1"
                     /translation="MTDRRAAARGKLIVALDVPSAADARRLVAALKGHVGLFKVGLEL
                     FVSAGPSLVDAIADLSDADIFLDLKLHDIPATMRAAIAAASKPRVRFLTVHCEQPDRL
                     VGAGTGQAAPQLLGVTALTSIGQAEMTAAGWFAPGLTVEELVLRRAAIARDAGCAGVV
                     CSGNEVRAIKRTLGRELMVVTPGIRPAWAAVPGDDQRRAVTPAEAIAAGADFIVVGRP
                     IRAAKDPGEAAERVVDEIEETLGQAG"
     gene            complement(4165..>5332)
                     /locus_tag="AC16BC_12115"
     CDS             complement(4165..>5332)
                     /locus_tag="AC16BC_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3242404.1"
                     /translation="LARRLAEPRRFIQAVAGPRQVGKTTLVRQVIEATGGEARFASAD
                     EPTLRGREWIEQQWEAARLTARDAGKSGAVLVLDEIQKMSGWSETVKRLWDEDTRARR
                     PLKVVLLGSAPLLIARGLTESLAGRFEVLRLPHWSFAEMKAAFGWSLDTYLFYGGYPG
                     AAPLIREHDRWAHYIIDSLIETTISRDVLLLSRVDKPALLRRLFHLGCRYSGQILSYT
                     KMLGQLHEAGNVTTLAHYLDLLAGAGMITGLQKFAGDVARQRGSSPKLQVLNTALMTA
                     QSGMTLKEARGDREFWGRLTESAVGAYWANAAATGACEVFYWRDRDKEVDFVVRAGRG
                     LVAIEVKSGRVREAPPGLAAFTQAFKPKRTLLIGDDGIPVERFLSRPTEDWVKA"
BASE COUNT          934 a         1838 c         1651 g          909 t
ORIGIN      
        1 ctgcatcgcc aactatcagg gcgatcccga ggtgttcctg acgatgcacg gcgaacgact
       61 ggtcggcctc atcaaggacg agcggctgcc gctcgacgaa gaggaggtcc ggtccaccct
      121 tcaggccagc atcaagtacg ggaaggacga cctgacgatc gtggactggg atggcgcgat
      181 tctcttcgat ccgcacgggg atttcgacgc gaacatcgag ttgatcgaaa tcgccaattt
      241 tcaattgctg cgaacgcgga tggcggacca ggcgctcgat gcgcggctcg cccagatcgc
      301 gcagatgctc cgcacgacgc ccaaacagtc tctgttccga cggcgggagg tccgggccat
      361 gttgcgcgag atcctccgca ttcgcaccga aaccctgctg gaatccccgg cgacggagca
      421 tcacatcaag atgatcggcg actggtatgc cgcgcgcgtg tacggcctgc tggccaggaa
      481 attccacatc gaggtatggc aaacgtccat ccaacacaag ctggacacga tcgaagacgt
      541 ctccgccatg gtcgcagaaa acttctcggt gtcgttccat accacgctgg acttcatctt
      601 gatcgccggg tggttcttgc tgttggcggg ctggttcgcg tacctcttcg tcgacgtgta
      661 tctgctcagc acccgctgaa cgggcgcccg aaggccttga caggagccgt gtctcgcgag
      721 tttcatgttc gctgaacccg gatgaccgca tgatcgaccg cgatgccaaa ttccacggat
      781 aacgcgccgg ccccggttat tgagttggcg ggcgtgacca agcgctacca gcccgaccac
      841 gctcccgcga tcgacgcgct ttcgctgacc gtggcgcgcg gccacaccct ggccgtgttg
      901 gggcccagcg gatgcggcaa aaccacgctg ctgcggctca tcgccggctt cgaagacccg
      961 tcatcgggca tggtccgact cgggggtcac tgcgtggcgg gcgcgggctg ctgggtgccg
     1021 ccggaacgcc gcggggtggg catggtgttt caggactacg cgctgtttcc tcacttgacc
     1081 gtctcgcgga acatctcgtt cggccttcat caaatggatc gaagcgcccg tgcgagccgg
     1141 gtgtccgagg tcctggaatt ggtcggtctc gcggggctcg aagaccgata tccgcaccag
     1201 ctctcgggcg gccagcagca acgcgtggcc cttgccagag ccctggcacc caaaccgatc
     1261 gtggtcctgc tcgacgagcc gttctccaac ctcgacccgg acatgcgctc acggatgcgc
     1321 caggaggtcg acgcgatctt gcgccaatcg ggcgcgacca cggtgttggt cacgcacgat
     1381 cacgaagaag ccttcgccat ggcggaccgc gtcgcggtct tgaaccgcgg acgcctggag
     1441 caactcggca cgcccgaggt ggtctaccac cttccgacca cgccgttcgt ggcggaattc
     1501 gtgggccagg ccgacttcgt gcccggggcg gtcgaagggc tgcaggtccg gaccgaattg
     1561 ggcccgttcc ccaacacctc gggattcccg accggcatgg ccgtctccgt gatgatccgg
     1621 cccgacgacg tcctgctcgt gccctcggac gatgcacccg cgatcgtggt caaccgacag
     1681 ttccgcggct cggagaatct ctacaccgtc cacctcgcct ccggccgccc gcttcactcc
     1741 acgcaaccgt cggacgtggt ctatgaactc ggcagccggg tggagatcaa aatcaacgcc
     1801 acgcacaccg tgctgttcga cccctcggtc ctgccgaagc acagcccgtc gcatcatgaa
     1861 gtctgaccgc cggccgtcgg aggcccgatt gactcggacc gggccgatgc gtacaatgca
     1921 gcttcttgat ttggccgtgc tcgggcgatt agctcagtgg tagagcgctt gcttcacacg
     1981 caagaggccg ctggttcgat accagcatcg cccaccatgc cccgcccccg gcgggcggcg
     2041 aaatgaggcg ctgagttccc gcgccgaggc ttcgcgaaac agttgacctt cgacgaattg
     2101 tctctcttcc gacgcaggat cgtcgtcgta atttgccgcg tacttgacgc cgcggcaggc
     2161 ctcagctctt gcgcttgatc ccgctgtgtt cctgaaactc ccgctcggcc ttcgcgggat
     2221 cgagcgagtt acggagcacc aggttttgaa ggtggataaa gctgtccgta tccatttcgc
     2281 taaattcgat ccccatcccc tccttgtccg cgtagacgat cctccccaac acccgcacgg
     2341 acatcggctc ccctccaccg gtcaggaaga tcgtgatcgg acattccgta ctggcgggaa
     2401 gctgccgcgg gccggatacg tacacgccct tcacgctgag gtcgcggcct cgccccgaga
     2461 tcttgggttg cccgggcggg gtcaattcca ccgcgactga aatcttaacc cgcgtgaact
     2521 ctcgacgatt cgcatagttc tccatggccc cacctttccg ccggccccga cccggctcat
     2581 ccctgacgcg gaaagtctat gccccagcgg gcgagccgtc aacgcggcgt cgcccgcgtc
     2641 catcgcggct tgcttagcac ctcgcggtct gagacaatga accacctttg gactggaact
     2701 gacgcatgaa acacgccacg gaccgcgcca tccccgactc gactctccag gacgccccca
     2761 tcgtcaggct caccggccag gccatcgcca aggtcgcgtc ggcattagcg gggaccgcga
     2821 acatcggggt acggctgacc gtggcgcggg agaaaggcgg cttcacctac aaattcgacg
     2881 tcgtcgcccc cgggcaggtc gatccgcgcg atcccaccgt cccttgcggc gcgtggacgt
     2941 tctacctgaa tcacgcgacc gtggaatgga ttcgcggcgc ggagatcgat tacatgtcgt
     3001 cgggattcac gcagggctgg gtgatcgata acccgaaccc ggcgtgggac agcgaactgg
     3061 cccgccggat cagcgaggtc ttcgacaaga ccatcaaccc cggcctcgcc caacacgggg
     3121 gccgcgtcag gctcgtggat ctgcaaggca cggtcgccta cgtggaaatg tccggagggt
     3181 gccaggggtg cagcatggcg accaagacgc tgcgggacgg gatcatgcgg gtgctcgccc
     3241 acgagttccc cgaactgacc ggcgtggtgg acaccaccga ccacgcgggc ggctcttcgc
     3301 cctatttcaa gggcgaccag cagggcgcgt cgcccacgct cggatgagcc ctcggccccg
     3361 gctccgacgc atcgtatgac cgaccgacgg gccgcggcca ggggcaagct gatcgtcgcg
     3421 ctcgacgtcc cctcggccgc cgacgcccga cgtctggtcg cggcgctgaa ggggcacgtc
     3481 ggcctgttca aggtcggcct ggaactgttc gtgagcgccg gcccctcgct cgtcgacgcg
     3541 atcgcggatc tcagcgacgc cgacatcttc ctcgatctga aactccacga catccccgcc
     3601 accatgcgcg cggccatcgc ggccgcttcg aaaccccgcg tgcgtttcct cacggtgcat
     3661 tgcgagcaac ctgaccgact ggttggagca ggcacaggcc aggcggcccc ccagttgctc
     3721 ggcgtcaccg ccttgacgag tattggacaa gctgagatga ccgcggcggg ttggttcgcg
     3781 cccggtttga ccgtcgagga gctggtgctc cggcgcgccg ccatcgctcg cgacgcgggc
     3841 tgcgccggcg tggtctgctc cggcaacgaa gtccgcgcga tcaagcggac actcggccgc
     3901 gaactcatgg tcgtcacgcc cggcatccgc ccggcctggg ccgcggtgcc cggggatgac
     3961 caacggcgcg ccgttacccc cgccgaggcc atcgcggccg gtgcggactt catcgtggtg
     4021 ggtcggccga tccgcgcggc gaaagaccca ggtgaagcgg ctgagcgcgt ggtggacgaa
     4081 atcgaagaaa cccttggtca ggcaggctga cgccggctca gcgccaccca gaggaacgtt
     4141 gcgccgacac gacgagcctc ccgactacgc ttttacccaa tcttcagttg gacgcgagag
     4201 aaaccgctcg acgggaatgc catcgtcgcc gatcagaagt gtcctcttcg gtttgaacgc
     4261 ctgtgtaaac gccgctaatc ctggaggagc ctcgcgaaca cggccgcttt tcacttcgat
     4321 cgccacgagg cctcgccctg cgcgcacgac aaaatccact tccttatcgc ggtcgcgcca
     4381 gtaaaacacc tcgcaggcgc ctgtagccgc agcattcgcc caataggcgc cgactgccga
     4441 ttccgtgaga cgcccccaga attcgcggtc accgcgcgcc tccttgagcg tcatccccga
     4501 ctgcgccgtc atcagcgcgg tgttcaacac ctgcagcttg gggctagagc cgcgctgacg
     4561 cgcgacatcg ccggcgaact tttgcaggcc ggtgatcatc cccgcgccgg ccaacagatc
     4621 gagatagtgc gcgagcgtcg tcacgttccc cgcctcgtgc aactgaccga gcatcttggt
     4681 gtacgagaga atctggcccg agtagcggca tccgagatgg aacagacgcc gaagcagggc
     4741 tggcttgtcc acgcgcgaca ggagcaacac atcgcgcgag atcgtcgtct caattaacga
     4801 atcgatgata tagtgcgccc accggtcatg ctcgcggatc agcggggccg cgcccggata
     4861 gccgccgtaa aagagatagg tgtcgagcga ccagccgaat gcggccttca tctccgcgaa
     4921 cgaccagtgc ggaagacgca gcacctcgaa gcgcccggcc agactctccg tgaggccgcg
     4981 agcaatgagc agcggcgccg acccgagcag caccaccttg agcgggcgcc gggcgcgtgt
     5041 gtcctcgtcc cacagacgct tgacggtctc ggaccacccc gacatcttct gaatttcgtc
     5101 caagacgagc accgcgccgg atttgccggc gtcgcgggcg gtcagcctcg cggcctccca
     5161 ctgctgctcg atccactcgc gcccgcgcag cgtaggctcg tctgcactgg cgaaccgcgc
     5221 ctcgcccccc gtcgcctcaa tgacctgtcg gacgagcgtg gttttgccga cctgccgcgg
     5281 cccggccacc gcctggatga accggcgtgg ttcggccaga cggcgggcga gg
//
LOCUS       DBNGTT010000522         1970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2295962, whole genome shotgun sequence.
ACCESSION   DBNGTT010000522 DBNGTT010000000
VERSION     DBNGTT010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2295962"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="AC16BC_12120"
     CDS             <1..103
                     /locus_tag="AC16BC_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HIR3242405.1"
                     /translation="KGKDIEKAVLARAVRWHLEDRVLVWGNRTVVFA"
     gene            100..501
                     /locus_tag="AC16BC_12125"
     CDS             100..501
                     /locus_tag="AC16BC_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242406.1"
                     /translation="MSARIGRVVATVACVAWATVAVGADPNDHDRAYDLNREGMIAMS
                     EARFTEAIDAFRQAAALATDYSIMGRPLMYTPVFMSAWGAEKIGRTRQACAAYRDYLR
                     IAVEHPVEPTKVDHAKGFIAANCQDAAPGQP"
     gene            complement(580..1173)
                     /locus_tag="AC16BC_12130"
     CDS             complement(580..1173)
                     /locus_tag="AC16BC_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242407.1"
                     /translation="MNRILAVAPLFLWGVVTMGSVLPSAAWAQEAPPPPPAAEHVFAA
                     KFDEVWDAAQTFLKTRPMPIPIGSAEKEGDAGKPPHKGVISTQPLRYFKIMSATFPPR
                     QQDYRDTYTITVTPLPKGPPPPNPPIPGVPPLPADKPVDLTKVQVERKFEKFDKKVKL
                     WVDGDVAKDHAGVSASDLFTGIQYLLTPPPPPPPVEE"
     gene            1433..>1970
                     /locus_tag="AC16BC_12135"
     CDS             1433..>1970
                     /locus_tag="AC16BC_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HflX-like GTP-binding protein"
                     /protein_id="HIR3242408.1"
                     /translation="MPTAFSGAAWRGARRVALLGLRRPDEHPWAIDAAFDAIEAWLRV
                     TGRSVVYRVWQRRRAPDPATYIGRGKAVQLRELCERASVDGVLINGTLSRSQRDGLLR
                     LLARPVWDRTVWGVTQKPPVPVARARDLARAARRRHGTFTVVLCGEAGAGKSTLFAAL
                     TRGAVSPPSSWPVGIRASG"
BASE COUNT          312 a          609 c          716 g          333 t
ORIGIN      
        1 caagggcaag gacatcgaaa aggcggtgtt ggcgcgcgcg gtgcgctggc acttggagga
       61 tcgcgtgctg gtgtggggca accgcaccgt ggtgttcgcg tgagcgcgcg gatcggtcgg
      121 gtagtcgcga cggtcgcgtg cgtggcgtgg gccacggtcg ccgtgggcgc cgacccgaac
      181 gaccacgatc gggcctatga cctgaaccgg gaagggatga tcgcgatgtc ggaagcgcga
      241 ttcacggagg cgatcgacgc gttccgacaa gcggccgcgt tggcgaccga ttactccatc
      301 atgggccggc cgctgatgta cacgcccgtg ttcatgtccg cgtggggggc tgaaaaaatc
      361 ggacggacgc gccaggcttg cgccgcgtac cgcgactacc tccggatcgc ggtggagcac
      421 ccggtcgaac ccaccaaggt cgatcacgcc aaggggttca tcgcggcgaa ttgccaagac
      481 gccgcgccgg ggcagccgta aaagaacggg ccgatcgtat agcgatcggc ccgtgggcgg
      541 cgcggcagac gccggcgccg aacaacatgg atggttgcgc tactcttcga ccggcggcgg
      601 aggaggcggc ggggtcagga ggtactgaat ccccgtgaac agatccgacg cgctgacgcc
      661 cgcgtggtct ttggccacgt cgccgtccac ccagagtttg accttcttgt cgaatttctc
      721 gaatttgcgt tccacctgaa ccttggtgag atccacgggt ttgtccgcag gaagcggcgg
      781 gactccggga atcgggggat tcggcggggg cggacccttg ggcagtggcg tcaccgtgat
      841 cgtataggta tcgcgataat cttgttggcg cggcgggaag gtcgccgaca tgatcttgaa
      901 gtaccgaagc ggttgggtgg aaatcacgcc cttgtggggc ggcttgcccg cgtcgccttc
      961 cttctcggcg ctcccgatcg ggatcggcat ggggcgggtt ttcagaaagg tttgcgccgc
     1021 gtcccacacc tcgtcgaatt tggcggcaaa cacgtgctcg gccgcgggag ggggcggcgg
     1081 ggcctcctgg gcccatgcag cggacgggag cacgctgccc atcgtcacga ctccccacag
     1141 gaacaacggc gcaacagcca gtatccgatt catgaatctc cttcccacgc ccgcgagggg
     1201 acctaagaaa cgcctggacg ttgcccgcgg cacgagaaac cgccgcggtg gcggcccatt
     1261 ggcgtcgatc agaaggccta acatagccca tcctgtttca acgtgtcaag gcattcgtgg
     1321 ccgtgcagcg gccttccgat cgccgtccat tgaccgccgc ggatggtccc agtaggatgg
     1381 cgcgatgcgt agtgttcggc ggatacccgg gactcttcac gggggagaac cgatgccaac
     1441 ggccttctcc ggcgccgcgt ggcgcggcgc ccgtcgcgtc gcgttactcg gtctgcgtcg
     1501 tccggacgaa catccgtggg ccatcgacgc ggcgttcgac gcgatcgaag cctggctgcg
     1561 ggtcacggga cggtccgtgg tctatcgcgt atggcagcgg cgtcgggccc cggatcccgc
     1621 cacgtacatc ggtcggggga aagcggtcca acttcgggag ttgtgcgagc gtgcgagcgt
     1681 cgacggcgtc ctgatcaacg gcacgctgag tcgctcgcaa cgagacgggt tgctccggct
     1741 gctcgcccga ccggtgtggg atcgcacggt ctggggcgtg acgcagaaac cgccggtccc
     1801 ggtcgcgcgc gcgcgggatt tggcgcgggc ggcgcggcgc cgacacggga cgttcaccgt
     1861 ggttctgtgc ggcgaggccg gcgcggggaa gtccacgttg ttcgcggcgc tgacccgcgg
     1921 cgcggtgtcg ccgccttcgt cgtggccggt cggaatccga gcgtccggtc
//
LOCUS       DBNGTT010000523         1545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2934372, whole genome shotgun sequence.
ACCESSION   DBNGTT010000523 DBNGTT010000000
VERSION     DBNGTT010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2934372"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="AC16BC_12140"
     CDS             complement(<1..1082)
                     /locus_tag="AC16BC_12140"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009361 - succinate-CoA ligase complex
                     (ADP-forming) [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004775 - succinate-CoA ligase
                     (ADP-forming) activity [Evidence IEA];
                     GO_function: GO:0004776 - succinate-CoA ligase
                     (GDP-forming) activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3242409.1"
                     /translation="MSIVANAETRVVIMGGPAGVSAARRMGEFCHLIKKPLNVVAFVF
                     PPDAGKTVEVPCGAQINSLPVYRTVAEATANHPEINATVIYVGADRATKAAQDALADP
                     KIKLVSMITEGVPEKDAKIMAKEAKKRGKVFNGPSSIGILSAGEARLGVIGGEFNNLV
                     LSKLYRPGSFGVITKSGGLSNEIMWICSQFADGVTTAIGIGGDAYPGTDFVSYLEMFE
                     QDPQTKAVVIVGEMGGNLEEQAAAWYGAKKRRIKLIGVISGFCQEHLPKGMKFGHAGA
                     KEGLKGEGSARAKSDALRKAGAIVPDTFGALGPAIEETYREMVESGVIAPAADADAAS
                     LPKLPKTVDQAIKSGDVMIEPLIKTT"
     gene            complement(1267..>1545)
                     /locus_tag="AC16BC_12145"
     CDS             complement(1267..>1545)
                     /locus_tag="AC16BC_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HIR3242410.1"
                     /translation="SLHGIKRIIVGGAIANFTDVKKTFTGIIAGFRRAKADGKLKNVT
                     IFVRRGGPKEAEGLAAMRALADEGFDIQVYDRYTPLTDIVDFAIQGKS"
BASE COUNT          225 a          531 c          465 g          324 t
ORIGIN      
        1 atcgtggtct tgatcaaggg ttcgatcatc acgtcgccgc tcttgatcgc ctgatccacg
       61 gtcttgggca gtttgggcaa cgacgccgcg tccgcgtccg ccgcgggcgc gatcacgccc
      121 gactccacca tctcgcggta ggtctcctcg atcgcggggc ccaaggcgcc gaacgtatcc
      181 ggcacaatgg cgccggcctt gcgcaacgcg tcggacttgg cgcgcgcgct gccctccccc
      241 ttcaggccct ctttcgcgcc cgcgtgcccg aacttcatgc ccttgggcaa atgctcctgg
      301 cagaagccgg agatcacgcc gatcaatttg atgcgccgct tcttggcgcc gtaccaggcc
      361 gcggcctgct cctccagatt gccgcccatc tcaccgacga tcaccacggc cttggtctgc
      421 gggtcctgct cgaacatttc caggtaggac acgaagtcgg tcccgggata cgcgtcgccg
      481 ccgatcccga tcgcggtggt cacgccgtcc gcgaactggc tgcagatcca catgatctcg
      541 ttggagagcc cgcccgactt ggtgatcacg ccgaacgacc ccgggcgata cagtttgctc
      601 aataccaggt tgttgaactc cccgccgatc acgcccaggc gcgcctcgcc ggccgagagg
      661 atgccgatcg aggacgggcc gttgaacacc ttgccgcgct tcttggcctc tttcgccatg
      721 atcttcgcgt ctttctccgg cacgccttcg gtgatcatgg agaccagttt gatcttgggg
      781 tcggccaggg cgtcctgcgc ggccttggtg gcgcggtccg cgcccacgta gatcaccgtg
      841 gcgttgatct ccgggtgatt cgcggtggct tcggccacgg tgcggtacac gggcaaggag
      901 ttgatttgcg cgccgcacgg cacttccacg gtcttgcccg cgtccggcgg gaacacgaac
      961 gccaccacgt tgaggggctt cttgatgaga tggcagaact cccccatgcg cctggccgcg
     1021 ctgacccccg cgggtccgcc catgatcacg actctcgttt cagcattcgc cacgatgctc
     1081 atgctttgcc ctttcgtgca aacgcctgtc catgaccggc catctgcgtc gtttccgctg
     1141 cgcatccggt gctcacgtac gactcagtac gctgcgctcc ggtgctcgcg gcgccttgca
     1201 tctgaccgct cctgaccagg cgacactgcc tgccgttgat ttcgcttgtt gggtttcggg
     1261 agccgctcat gatttgccct ggatcgcgaa atccacgata tccgtcagcg gggtgtaccg
     1321 gtcgtacacc tgaatgtcga acccttcgtc ggccaacgcg cgcatcgcgg ccaggccctc
     1381 ggcttccttg ggaccgccgc ggcgcacgaa gattgtcacg ttcttgagtt tgccgtcggc
     1441 cttggcgcga cggaacccgg cgatgatgcc ggtaaaggtt tttttgacgt cggtgaagtt
     1501 ggcgatggcc ccgcccacga tgatgcgctt gatgccgtgc aggct
//
LOCUS       DBNGTT010000524         4586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1913338, whole genome shotgun sequence.
ACCESSION   DBNGTT010000524 DBNGTT010000000
VERSION     DBNGTT010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1913338"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..1549)
                     /locus_tag="AC16BC_12150"
     CDS             complement(263..1549)
                     /locus_tag="AC16BC_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HIR3242411.1"
                     /translation="MSSTPSRPGPQARAWVERDAKVVSPSYTRTYPLVIKHSAGLHVV
                     DVDGHVYLDFTSGLGTSLLGHCHPDVVQAITDQANSLIHMSGTDFYYPSQIRLAEELV
                     KHCPGDEPKQVFFSNSGAEAVEAAIKLARYYTKRQHLIGFYGGFHGRTLGALSLSASK
                     AIHRRGFGPLVPGVHHAPYGEIEFIEQHLFGRVVSPDEVAGIVVEPIQGEGGYVVPPD
                     GFLRKLRDLCTQHGILLIADEIQSGMGRTGKFLACEHEGIVPDIVAMSKGIAAGMPLG
                     ATIAPTRLMTWPSGAHANTFGGHPISCETALVALRHLFEGGLIRRAGQAGERLIAGLK
                     QTADRSPSIGEVRGKGLMVGVEIVKDRAGRKPSPEARNRIIQRCFEKGLLLVGAGKHV
                     VRFLPALVATDQQIDQAVAIFTDAVLEVDRELPHAA"
     gene            complement(1744..3879)
                     /locus_tag="AC16BC_12155"
     CDS             complement(1744..3879)
                     /locus_tag="AC16BC_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3242412.1"
                     /translation="MSAPTTDPAWVTGFLTSIVTDYLEERFPRVARDLPCPEFFADTS
                     LAHRAADARALVTDPNQWLPQEVLVRLVLAAEDATGEPDLAYHATRAHFERRHRRGPS
                     IFEIIATLLDDVRGVVIASNRWAGAFATNTALQALDHADRPEATLLYMVTSKGRLTRL
                     PGLFLRGQCEGFPALFGRPGSATFTEEFTQLSLAHLLREFTDYDLARDHERLRVVERS
                     SGSIVAEAEGVVLGTEQIPVSEHFPAEDGAIVSPAAGRYTVFTTTGATPAPLDRAHAV
                     RIVRGGALRRGGVTVSLEPGSVYGAPYTKARFRWSDQRGPRQPAEARLRDVSRLLFTH
                     LHEIKQTQRRLLRCERDNASLREENAHLKSELADRNRFTDLIAASPAMRTLCATLERV
                     VDADTTVLLLGETGTGKEVIARALHYNGPRKDARFVAVNCGALPEGLLESELFGHERG
                     AFTGALQRRIGKFEAADGGTLFLDEIGELSPALQVKLLRVLQERTICRVGGNDDVRVD
                     IRVIAATHRHLLDMVKAGTFRQDLYYRLNVMPIAIPPLRERREDVPLLAQALLDKLRT
                     RTGRASERFSPDALRVLGSHHWPGNVRELENVIERALILAGTESTITAAHLPTEWFGG
                     DRRDQAAAPGDPWTLIGKMDWSCFASFFDRGGSFDDLLQRIEWAITERAVQEHHGNKT
                     HAARTLKRSYRWLRKLEKESLDPGGPSDA"
     gene            complement(3860..4432)
                     /locus_tag="AC16BC_12160"
     CDS             complement(3860..4432)
                     /locus_tag="AC16BC_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019628492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HIR3242413.1"
                     /translation="MKRVLVPLAPGFEEIEAATIIDILRRADVHVTGAGTVDGPVTGS
                     RGVVITPDAALDAVADASFDMVVLPGGGPGTEALRRDARVASIVKRHADRGEWIAAIC
                     AAPTVLADAGLLAGRRVTSYPSVQSQLSATDYRTDRVVVDGRLITSRSPGTALEFALT
                     LVESLAGRTVSRRIAGDVLALGADRVGAHD"
BASE COUNT          734 a         1489 c         1608 g          755 t
ORIGIN      
        1 cagcagatcg agccgacgca gggcgtgttg atctcctgcc tgggctgcag cccgcataac
       61 ctgccgctgc agcaggacac ggtgacctac acgttcacgt ggcctggtct gccgaccgtc
      121 acgccggtgc tgcatccgcc ggcgaccgga gacacggtca tcagcgtggt gccgtaacac
      181 accagcaggt cgtaagacag gggaagcccg gcaacgggct tccccttttt ttgtccgcgg
      241 gattccgaag aggggggggc ggctaggccg cgtggggcag ttcgcggtcc acttccagca
      301 ccgcgtcggt aaaaatcgcc acggcctggt cgatctgctg gtcggtcgcg accagggcgg
      361 ggaggaaacg caccacgtgt ttgcccgcgc cgaccaggag caaacccttt tcgaagcagc
      421 gctggatgat gcggttccgc gcctccggtg acggcttgcg gccggcccgg tccttcacga
      481 tttccacgcc gaccatcagg cccttgccgc gcacttcgcc gatggacggg gaacgatccg
      541 ccgtctgctt caatcccgcg atgagccgtt cgcccgcctg gcccgcgcgc cggatcaggc
      601 cgccttcgaa taagtgacgc aacgccacca atgcggtttc gcacgagatc gggtgcccgc
      661 cgaaggtgtt ggcgtgcgcg ccggacggcc acgtcatcaa ccgcgtgggg gcgatggtgg
      721 cgcccagggg catgcccgcc gcgatgcctt tggacatggc cacgatgtca gggacaatgc
      781 cctcgtgttc gcacgcgagg aacttgccgg tgcggcccat gccgctctgg atttcatccg
      841 cgatcaataa gatgccgtgc tgcgtgcaga ggtcccggag cttgcgcagg aaaccgtccg
      901 gaggcaccac gtagccgcct tcgccctgga tcggctccac gacgatcccc gcgacttcat
      961 cgggagacac cacgcggccg aacaagtgct gttcgatgaa ttcgatctcg ccgtagggcg
     1021 cgtggtgcac accgggcacc agggggccga agcctcggcg gtggatcgcc ttgctggccg
     1081 agagcgacaa cgcgcccaga gtccggccgt ggaacccgcc gtagaacccg atgaggtgtt
     1141 ggcgtttggt gtagtaccgg gccagcttga tcgcggcttc aaccgcttcc gcgcccgagt
     1201 tggagaagaa gacctgcttg ggctcatcgc cagggcaatg cttgacgagt tcctcggcca
     1261 ggcggatttg cgacgggtaa tagaagtcgg tcccggacat gtggatcagg ctgttggcct
     1321 ggtccgtgat ggcttgcacc acgtcgggat ggcaatgccc gagcaacgac gtgccgagcc
     1381 cggaggtgaa atccaggtac acgtgcccgt ccacgtccac cacgtgaagt ccagccgagt
     1441 gtttgatgac cagcggatag gttcgtgtat aggaaggcga gacgaccttg gcgtctcgct
     1501 cgacccatgc gcgggcttgt ggtccggggc gtgagggcgt ggacgacatg gcagaaggag
     1561 tatagcagat agagtcgtcg tggccgggcg tggcgaatgc gtgaaagcgc gacgagccga
     1621 atcggggaat cggggtatcg gggaaacgga gaacgacgcc aagaaccaca caaatggttt
     1681 cgtcctgttc accgattctc cggttcaccg attccccggt tcggctctca gacccgtttg
     1741 cctttacgcg tcgctgggcc cgcccgggtc gagcgattcc ttttcgagtt tgcgcagcca
     1801 gcggtagctg cgtttgagcg tgcgcgcggc gtgcgttttg ttgccgtggt gctcctgcac
     1861 cgcgcgttcg gtgatggccc attcgatccg ctggagcagg tcgtcgaacg agccgccgcg
     1921 gtcgaagaag ctcgcaaagc aggaccaatc cattttcccg atcagggtcc acgggtctcc
     1981 tggtgccgcg gcctggtccc ggcgatcccc gccgaaccat tccgtcggca gatgcgcggc
     2041 cgtgatggtc gactcggttc cggccaggat cagcgcgcgc tcaatcacgt tctccagttc
     2101 ccggacgttg ccgggccaat ggtggctacc gagcacccgc aaggcatcgg gcgagaaccg
     2161 ttcggaggcc cggccggtgc gggtcctgag cttgtcgagc agggcctgcg cgagcagggg
     2221 gacgtcctcc cgccgctcgc gcagcggtgg aatcgcgatc ggcatcacgt tgaggcgata
     2281 gtagaggtcc tgtcggaagg tgccggcttt gaccatgtcg agcagatggc gatgggtggc
     2341 ggcgatcacg cggatgtcga cccgcacgtc gtcattgccg cccacgcggc agatggtgcg
     2401 ctcctgtagg acccgcagca gtttgacctg gagagccggg gagagttcgc cgatctcgtc
     2461 caggaacagt gtgccgccgt cggcggcttc gaacttgccg atccggcgct gcaacgcgcc
     2521 ggtgaacgcg ccgcgctcgt ggccgaacaa ctcgctttcc agcaaccctt cgggcagggc
     2581 cccgcagttc acggccacga agcgcgcgtc ttttcgaggc ccgttgtagt gcagggcgcg
     2641 ggcgatcacc tccttgcccg tgccggtttc gcccaggagg agcacggtcg tgtcggcgtc
     2701 caccacgcgc tccagcgtgg cgcacagggt ccgcatcgcg gggctggccg cgatcaggtc
     2761 ggtgaaccgg ttgcggtcgg ccaactccga tttgaggtgc gcattctctt cgcggagcga
     2821 ggcgttgtcg cgctcgcagc gcaggagacg gcgctgggtc tgcttgatct cgtgcaggtg
     2881 ggtaaagagc agccgcgaca cgtcccgcag gcgggcttcc gcgggttggc gcggtccccg
     2941 ctggtcggac cagcgaaagc gtgccttcgt gtacggcgcg ccgtacaccg agcccggttc
     3001 gagcgacacg gtcacgccgc ctcggcgcaa cgcgccgccg cgcacgatgc gcacggcgtg
     3061 cgcccgatcg agcggagcgg gcgtcgcgcc ggtcgtcgtg aacaccgtgt agcgcccggc
     3121 ggccggggaa acgatggcgc cgtcttcggc gggaaagtgc tcgctcaccg gaatctgttc
     3181 ggtgcccagc accacgccct cggcctcggc tacgatcgaa ccggaggacc gttcgaccac
     3241 gcggagccgc tcgtggtccc gggccaggtc atagtcggtg aactcgcgca gcaggtgcgc
     3301 gagcgagagt tgcgtgaact cctcggtaaa tgtcgcggac cccgggcgtc cgaacaaggc
     3361 cggaaaccct tcgcactggc cgcgaagaaa caggccggga agccgggtca accggccctt
     3421 ggacgtgacc atgtacaaga gggtggcttc ggggcgatcc gcgtgatcca gcgcctgcaa
     3481 cgcggtgttg gtggcgaacg cgcccgccca ccggttcgac gcgatgacca cgccgcgcac
     3541 gtcgtccagc agcgtggcga tgatctcgaa gatcgacggc ccccgtcggt gcctgcgctc
     3601 gaagtgcgcc cgggtcgcgt ggtacgcgag atccggttcc ccggtggcgt cctcggccgc
     3661 gagcaccaga cgcaccagca cctcttgcgg gagccactga ttcgggtccg tgaccaaggc
     3721 ccgcgcgtcc gcggcccggt gcgcgaggga ggtgtcggcg aagaactcgg gacacggcag
     3781 gtcacgcgcg acccggggga aacgttcctc caggtagtcg gtgacgatgc tggtgaggaa
     3841 tccggtgacc caggccgggt cagtcgtggg cgccgacacg gtccgctccc aacgccaaca
     3901 cgtcgcccgc gatgcggcga ctcacggtcc gtcccgccag ggactccacg agggtcaggg
     3961 cgaattccag cgcggtgccg ggcgagcggc tcgtgatgag ccgtccgtcc accaccacgc
     4021 ggtcggttcg gtagtcggtt gccgagagct gggattgtac cgagggataa ctggtcacgc
     4081 gccggcccgc gagcaggccc gcgtcggcga gcaccgtggg cgcggcgcag atcgccgcga
     4141 tccactcgcc gcggtccgcg tggcgcttca cgatcgacgc cacgcgggcg tcgcggcgca
     4201 acgcctcggt gccggggccg ccgccaggca gcacgaccat gtcgaaactg gcgtcggcca
     4261 cggcgtcgag cgcggcgtcg ggggtgatca cgaccccgcg gctgcccgtg accgggccgt
     4321 cgacggtgcc ggccccggtc acgtggacgt cggcccgcct gagaatgtcg atgatggtgg
     4381 cggcttcgat ttcttcgaag cccggagcga gtggaacgag gacgcgtttc atggcagacc
     4441 tcgcgttgtg agggagggac acgcgatggt gctcccgtcc cgcgtggacc gactcgtgcc
     4501 cgcgtgttgc cgggatcgtt ccacggaaca cgcggagcgg cacgatcatt caacgtagtg
     4561 ggccgatacc actccttccg tgtatg
//
LOCUS       DBNGTT010000525         5487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_764135, whole genome shotgun sequence.
ACCESSION   DBNGTT010000525 DBNGTT010000000
VERSION     DBNGTT010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_764135"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2536)
                     /locus_tag="AC16BC_12165"
     CDS             complement(<1..2536)
                     /locus_tag="AC16BC_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HIR3242414.1"
                     /translation="MFLAAFLSIPCSSAQARTPDRPSDAAIRQVDELSALWSFYKHTY
                     IATGRVISLDEQGVTTSEGQGYAMLRAVWSNDRLAFASAWQWTKQHLQVRNDHLFAWK
                     WKGQVLSLNSATDADTDIALALILASRRFSEPAYEHAALDVLDSIWNKEIAKVGRQYY
                     VTAGNWAVNEDYPAIHVAYLAPYAYEIFASVDDRYPWKRLVDSSYEILHWIYFEQGLR
                     LPPEILFLDKQTGRLLLKHPRTGESASFSYDAFPIFWRVALDARWFGRPQSELRRKML
                     AFFQDEWRTRKAFFDRYSIRGEPLSRLEGLPLYATVHALALEEDQVLAGHISRAKLDP
                     LRQKALGGNGTPYYLHNWLWFGRAFELQALRTYDEFLGFLRPFDFKGFSAHFPWALFA
                     VTIALFFLVRVHPRFKLAFLVCGFSLCGYYLVWRLFNTLNFVESAGPFISLSLWGAEL
                     YAFSTVLLLLVQVGLGGKQKPQGAQPRPGFSPSVDILIPIYSESCEILAKTLVAARAI
                     DYADKRIYVLDDGHRPEVARVARRFGATYIEGPRTHAKAGNLNHALGQTDGELVAVFD
                     TDHIPVTTFLQETVPFFVDPKMAVVQTPHHFYNPDIFQRAFGGSERIPNEQDMFNHAI
                     QNGRDAWNGAFFVGSGAVFRRSALADVNGFNLMSITEDIHTSQHLHAAGWRSAFLDKD
                     LAVGLAAENLSSYLGQRRRWMLGCLQIFFKDNPLIRQGLTTRQRLGYFASLYYFFFPV
                     ARVVFWITPLYFLLFHFHPILSDVSVLVAYLLPFIIILPLIASTLVPGWPRLFWASVY
                     EAAVSFQLFRSMFDLFLPKQLGFKVTPKGLRSHTRAFDVNSATLPLI"
     gene            complement(2642..3676)
                     /locus_tag="AC16BC_12170"
     CDS             complement(2642..3676)
                     /locus_tag="AC16BC_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIR3242415.1"
                     /translation="MAKMRVIQIAQAKGPFELKERDIPIPGPGTVRIKVQACGICHSD
                     VYTKDGLWPGIQFPRVPGHEVAGVIDALGDGVGGWTVGQRVGVGWHGGHCGYCESCRR
                     GQFVTCQINNTVTGISIDGGYADYMLAPSGVLAKIPDALTPEEAAPLMCAGVTTYNAL
                     RNSGARPGDSVAVLGLGGLGHLGVQYAAKMGMKTIAVARGKDKEPLAKKLGAHHYIDS
                     QATDPAAELMKLGGANYILATVTDAKAMNAVMGGLSLNGRLIIVGAPHEPLSVSAGFL
                     IGGRRSITGCPSGSSIDSQDTLAFSALTGVRSMNEVFPLERASEAYDRMMSGKARFRV
                     VLTTGQQGAR"
     gene            complement(3669..4913)
                     /locus_tag="AC16BC_12175"
                     /pseudo
     CDS             complement(3669..4913)
                     /locus_tag="AC16BC_12175"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
     gene            complement(5070..5258)
                     /locus_tag="AC16BC_12180"
     CDS             complement(5070..5258)
                     /locus_tag="AC16BC_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242416.1"
                     /translation="MSTPPTSTPKGFAFRPDPDTLKACRGMSAEDKLRWLEEANEFVR
                     RFVPPERLEQWERIRRGG"
     gene            complement(5255..>5487)
                     /locus_tag="AC16BC_12185"
     CDS             complement(5255..>5487)
                     /locus_tag="AC16BC_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242417.1"
                     /translation="SFFHPVSQGKLIDVFIGEPIAFQEVDQAKTMVKAADLSIPVISI
                     PHLKQLKRIAGRSQDLADIEALEAIERMGGAS"
BASE COUNT         1061 a         1650 c         1803 g          973 t
ORIGIN      
        1 cgatgagggg gagggtcgcg gagttcacgt cgaacgcccg cgtgtgggac cgcaagccct
       61 tcggagtgac cttgaagccc agttgtttgg gcaggaagag atcgaacatg gaccgaaaga
      121 gttgaaagct caccgcggct tcgtacaccg aggcccagaa cagccgcggc cagccgggca
      181 cgagcgtgct ggcgatcagc ggcaggatga tgatgaaggg cagcaggtac gcgaccagaa
      241 ccgagacgtc cgagagaatg ggatggaaat gaaacaatag gaaatagagc ggcgtgatcc
      301 agaacacgac ccgcgccacg ggaaagaaga aatagtacag cgacgcgaaa tagcccagcc
      361 gttgccgtgt ggtcaggcct tggcggatca ggggattgtc cttgaaaaag atctgcaggc
      421 agccgagcat ccagcgcctc cgttgtccga gatacgacga cagattctcg gccgcgagcc
      481 cgaccgccag atccttgtcg agaaacgccg agcgccatcc cgcggcgtgc agatgctgac
      541 tggtgtggat gtcctcggta atgctcatga gattgaagcc gttgacgtcg gcgagggccg
      601 agcgtcggaa cacggcgccg cttcccacga agaacgcccc gttccaggca tccctcccgt
      661 tctgaatcgc gtggttgaac atgtcctgtt cattcggaat ccgctcgctg cccccgaacg
      721 ctcgttggaa gatgtccggg ttgtagaagt ggtgcggggt ctgtaccacc gccattttgg
      781 gatcaacaaa gaacggcacg gtctcttgca ggaacgtggt cacggggatg tgatcggtgt
      841 cgaacaccgc cacgagctcg ccatcggtct ggcccagcgc gtggttgaga ttgccggctt
      901 tggcgtgcgt tcgcgggcct tcgatgtacg tggcgccgaa tcgccgcgcg acgcgggcca
      961 cctcgggccg gtggccgtcg tccagcacgt agatccgttt gtccgcgtag tcgatggcgc
     1021 gtgccgcgac cagggttttc gcgaggatct cgcacgattc ggaatagatc ggaatcagga
     1081 tgtcgacgga cggcgagaag cccggccgcg gttgcgcgcc ctgcggcttc tgcttcccgc
     1141 ccagccccac ctgtacgagg agcagcagca cggtggaaaa ggcatacaac tccgcccccc
     1201 acagcgagag gctgatgaac ggtcccgcgg attccacgaa attcagcgtg ttgaagagcc
     1261 gccagaccag gtagtacccg cagagggaga acccgcacac cagaaacgcg agcttgaaac
     1321 ggggatgaac gcggacgagg aaaaacaggg cgatcgtcac cgcgaacaat gcccagggga
     1381 agtgagccga gaatcccttg aaatcgaacg ggcgcagaaa tcccagaaat tcatcgtacg
     1441 tccgcaacgc ttggagttcg aaggcgcgcc cgaaccacag ccagttgtgc aggtaatagg
     1501 gcgtgccgtt cccgcccagg gccttctggc ggagcgggtc cagcttcgcc cggctgatgt
     1561 gcccggcaag aacctggtcc tcctcgagtg cgagcgcgtg gaccgtggcg tagaggggaa
     1621 gcccttcgag ccgagacagc ggctcaccgc ggatcgagta tcgatcgaag aacgcctttc
     1681 gggttcgcca ctcgtcctgg aaaaacgcga gcatcttgcg acgcagctcg ctctggggcc
     1741 gaccgaacca ccgggcatcc agcgcgaccc gccagaagat cggaaacgcg tcgtaactaa
     1801 aagacgccga ctctccggtg cgggggtgtt tcaggagaag tcggccggtc tgcttatcca
     1861 gaaacagaat ctcgggtggg agtcgaagtc cttgctcgaa atagatccaa tggaggattt
     1921 cgtaagagct gtcgaccagc cgtttccagg gatatcgatc gtccacgctc gcgaagatct
     1981 cgtacgcgta cggcgccagg tacgcgacgt gaatcgcggg gtagtcttcg ttcaccgccc
     2041 agttgccggc ggtcacatag tactgacgac cgaccttcgc aatttccttg ttccagatgg
     2101 aatccagcac gtccagcgcc gcgtgttcat aggcgggctc ggaaaaccgc cgcgaggcca
     2161 ggatcagcgc cagcgcaatg tcggtatcgg cgtcggtggc tgaattgagc gagagcactt
     2221 ggcctttcca cttccaggca aagaggtgat cgttccggac ctgaagatgc tgcttggtcc
     2281 actgccatgc gcttgcgaag gcgagccgat cgttcgacca caccgcgcga agcatggcgt
     2341 agccttggcc ttcggacgtg gtgacgccct gttcgtccag gctgatcacg cggccggtcg
     2401 cgatgtaggt gtgcttgtag aagctccaca atgcggagag ttcgtccacc tgccggatcg
     2461 ccgcgtcgct ggggcgatcg ggtgttcgtg cctgcgcgga cgaacagggg atcgacagga
     2521 aggccgcaag aaacacgagg cctcgaaccg aaaatgagcg agttgtcatt gcctaacttt
     2581 agggaggaac cttcggttca accgctactt cggaacgaca cgtcggggga cagatttctg
     2641 ttcatctcgc gccttgctgc ccggttgtca gcaccactcg gaaccgtgcc tttccactca
     2701 tcatccggtc ataggcctcc gaggcgcgct ccaaggggaa gacttcgttc atcgagcgca
     2761 cgcctgtcaa tgcgctgaac gccagggtgt cctgcgagtc gatggaggag ccggagggac
     2821 agccggtgat ggatcggcgg ccgccgatga ggaagccggc ggacacagag agcggttcgt
     2881 gcggcgcgcc cacgatgatc agtctcccgt tcaggctcag cccgcccatg accgcgttca
     2941 tggctttggc gtccgtgacc gtggcgagaa tataattcgc gccgcccaat ttcatcaatt
     3001 ctgcggccgg gtccgtggcc tggctgtcga tgtagtggtg cgcgccgagt ttcttggcca
     3061 agggctcttt gtctttcccg cgcgcaacgg ctatggtctt catgcccatc ttggccgcgt
     3121 attgtacgcc caaatgaccg aggccgccta ggcccagcac ggccacggaa tcaccaggcc
     3181 gcgcgccgct gttgcgcagg gcgttgtagg tggtgacgcc ggcgcacatc aggggcgcgg
     3241 cttcttctgg ggtgagggcg tcggggattt tggccaggac gccggagggc gcgagcatgt
     3301 agtcggcgta gccgccgtcg atggagatgc cggtgaccgt gttgttgatc tggcaagtca
     3361 cgaattggcc gcggcggcag gactcgcagt agccgcagtg tccgccgtgc cagccaacgc
     3421 ccacgcgctg tcccacggtc cagccaccca cgccgtcgcc caaggcatcg atcacgcccg
     3481 ccacttcatg ccccggaacg cgcgggaatt ggatgccggg ccagagcccg tctttggtgt
     3541 acacgtcgct gtggcagatg ccgcaggcct ggaccttgat tcgcaccgtg ccgggtccgg
     3601 gaatggggat gtcgcgctcc ttcagctcaa agggaccttt ggcttgggca atctgtatga
     3661 cgcgcatctt agccatggct cttctttgcc tcatcttgcg gttcgttcac cagggattgc
     3721 aaggtccatc cagcgagggc cttttcaacg gcttgatcgc ggcggcgaag cgcgtccgcg
     3781 accggatctt ttgcgtgcgt cggaatcaag acttctgacg ctttcacctc ccgaatcgca
     3841 tccaggactt cgtgaagggg cgtggactcg ggcggtttga gcagggtcac gccttggggg
     3901 tcggtggtgc ggccgagcac gccgtgtttg acgagctggt ccacctgctc gtccaccagg
     3961 gacgcggcca gatggaggtc gcgggccagg tcggccgggc ggacggggcc ttcgcctttg
     4021 agaaaccggc gcgtgagcgc ggccgccacc atgagcgcga tgcgttctcg cagggccggg
     4081 gtctggtgct gccacatcat ggtggattgg tacgcggtgg ggtgttggtg gaagaacgcc
     4141 acttgggcgc ccaccagcac gatcagccac gcggcgtaga gccagatcaa cagcaatagc
     4201 acgatggcaa acccggagta gatcgcgccg tactgcccgg acccggccac gaatttcgcg
     4261 aacgccatgc ccgcgaggcc ccacaatgcg gcggcggaga tcccgcccac cagggccgag
     4321 cccaggcgca cctcggtgtt gggcacgaac ttgtagatga aggagaacac acccgcgagc
     4381 aggaggaacg gcacgagctt ggccagtgtc agcaccgacg atcccagcgg ctcgaagtgc
     4441 agcatgcgct ggaccagggt ctggctctgg accgaggcca ggagccccag ggccgtgacc
     4501 accagcacgg gcccgaccag gaccacgctc agattgtcgg tgaacttccg ggtccaggat
     4561 cgcccgtggc gcacctgcca gatggcgttc tacgactgct cgaccttgtc gatgagcgag
     4621 aaggtggtgt acaagaggcc cgcgaccccg agcacgccga gcacgccgat cttgaggttg
     4681 tccacgaacg caatcacctg ttgggtgatt tcctgtccct tgggccccaa cggctccagc
     4741 aattgcgcca gcacgggttc gatctggtgg tgcacgccga aggccttcaa gaccgaaaac
     4801 accacggcga gaaacggcac caatgacagg agggtggtgt agaccaggcc ggcggcgcgc
     4861 gcgtcgagca agcgatggcg aaattccaac gacaccgcgg cggccaggcg catggcctgc
     4921 accgccacgc gattcacgcc gtggagcgac cggagatcgg cggtccagag gttgcgctgg
     4981 acgaattgtt tgatgtgcag caccgcgacc cttccagtcg cgaccaacgg gagcaggtcg
     5041 atcggttacg tgtcagccgt tgtgacggtc tagccgccgc gcctgatccg ctcccattgt
     5101 tccaggcgtt ccggagggac gaaccgacgc acgaattcgt tggcttcttc cagccagcga
     5161 agcttgtcct ccgcggacat gcctcggcag gccttgagcg tgtctggatc gggtcgaaac
     5221 gcgaatccct ttggggtgct ggtcggcggc gtgctcatga ggcaccgccc atacgctcga
     5281 tggcttcgag cgcctcgata tcggccaggt cctgcgatcg gcccgcgatc cgtttgagct
     5341 gcttgaggtg aggaatggag atgaccggaa tgctgaggtc cgcggctttg accatggtct
     5401 tggcctgatc gacttcttga aacgcgatgg gctcgccgat gaagacatcg atcagtttcc
     5461 cctggctcac cggatggaag aaactga
//
LOCUS       DBNGTT010000526         1660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_508340, whole genome shotgun sequence.
ACCESSION   DBNGTT010000526 DBNGTT010000000
VERSION     DBNGTT010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_508340"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC16BC_12190"
     CDS             <1..354
                     /locus_tag="AC16BC_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242418.1"
                     /translation="QRSGHALFVQTMTATPVYRFAGSDAVGAWQVYGGVGVGRYGLTA
                     RFDFQDTGVTTEFDTYTLGYQALIGAEYRLNEQAGLLIEEKFSRARIRFGPELGRNEI
                     DVGGHNILVGARIHF"
     gene            complement(568..981)
                     /locus_tag="AC16BC_12195"
     CDS             complement(568..981)
                     /locus_tag="AC16BC_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242419.1"
                     /translation="MLRTMWRAALRGLAVGAVVWLVAAGPAAAAERHPALLAMGEMMT
                     VARAMADAGERGDLAGLVADAARVIDAGARALDGLPKPGNRHARDAADHVREAIESAR
                     LAAEAGTQGRRDEAVAQVKRTMSHIRQGAGHAEAL"
     gene            complement(987..>1660)
                     /locus_tag="AC16BC_12200"
     CDS             complement(987..>1660)
                     /locus_tag="AC16BC_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3242420.1"
                     /translation="DTIYRADWEASRWVPHGDGLSGSPTALLVGGGRLYAVTTSGLFT
                     RKSGDATWTGLDLPARTEPILHAVDRNGRAVVAVGRAVFRGEEDGGRWTPLGEPSGSD
                     ELIGVAAGRGEYAWTRRTVYAHRGRSTDWEDIGATLPSGVDIRAVAVDSGGRRDVLLA
                     ATSGGIWWRDDGGDWNPAHGASSIVPFESVLVPETGLVMAGSAEHGVFVGVNLVPKQG
                     FFGGE"
BASE COUNT          259 a          635 c          496 g          270 t
ORIGIN      
        1 cagcggtcag ggcacgccct cttcgtgcaa accatgaccg ccacgcccgt gtaccgcttc
       61 gccggatccg atgcggtggg cgcgtggcaa gtgtacggcg gggtgggcgt gggacgctac
      121 ggcttgaccg cccgatttga ttttcaggac accggcgtca ccaccgaatt cgacacctac
      181 accctgggct accaggcgtt gatcggcgcc gaatatcgac tcaacgagca ggctggatta
      241 ctcatcgaag aaaaattttc ccgcgcgcga atccgattcg gtccggaatt ggggcgcaat
      301 gaaatcgacg tgggtgggca taacatcctg gtcggcgccc gcattcactt ctagaggaag
      361 ctgctgaaaa agtccatctg cggcggtgat gtccgaggag tcccggccgc ctcaccgact
      421 cggcggcgct catggcttgg cgccacttat tcgtggcgcc actcgcccca cgtacgactc
      481 agtacgctcc gggttcctcc tccctgcggc cttgcatctg gaactttttg agcagcttct
      541 tgttgcgctt tcttttgtga ttccggatta cagcgcttcg gcgtggccgg cgccttgccg
      601 gatgtggctc attgttctct tcacctgggc cacggcttcg tcccgacgtc cctgggttcc
      661 cgcctcggcc gcgagacgag cggactcgat cgcctcgcgc acgtgatcgg ccgcgtcccg
      721 cgcatgacgg ttcccgggct tgggcaaccc atcgagcgcg cgtgctcccg cgtcgatcac
      781 cctcgcggcg tcggcgacca agcccgccag atcaccgcgt tcacccgcat ccgccatggc
      841 gcgggccacg gtcatcatct cgcccatcgc gagcaacgcc ggatggcgct cggctgcggc
      901 cgccggcccg gccgcaacca accagaccac cgccccgacg gccagccccc tcagcgccgc
      961 tcgccacatg gttcgcaaca cccgggtcac tccccgccga agaatccctg tttggggacc
     1021 agattgacgc ccacgaacac gccgtgctcc gcggatcccg ccatgaccag cccggtctcc
     1081 ggcaccagga ccgactcgaa cggcacgatc gacgatgcgc cgtgagcggg gttccagtca
     1141 ccgccgtcgt cccgccacca gatccctccg ctggtcgcgg ccagcagcac gtcacgacgt
     1201 ccgccgctgt ccaccgccac cgcccggatg tccacgccgg acggcaacgt ggccccgata
     1261 tcctcccagt ccgtcgagcg gccccgatgc gcgtacacgg tgcgtcgcgt ccacgcgtat
     1321 tcgccgcgac ccgcggccac gccgatcaac tcgtccgacc ccgacggctc gcccagcggg
     1381 gtccagcgac cgccgtcctc ctcgccgcga aagaccgcgc gcccgaccgc cacgaccgcg
     1441 cggccgttgc gatccaccgc gtgcaggatc ggctccgtcc gggccgggag atccaggccg
     1501 gtccacgtcg cgtcgccgga cttgcgggta aacaggccgg acgtggtgac cgcgtacaac
     1561 cgtcccccgc cgacaagcaa cgcggtcggg ctgcccgaca gcccgtcgcc gtgcggcacc
     1621 caacgactcg cctcccagtc ggcccgataa atcgtgtccg
//
LOCUS       DBNGTT010000527         2145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2170689, whole genome shotgun sequence.
ACCESSION   DBNGTT010000527 DBNGTT010000000
VERSION     DBNGTT010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2170689"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="AC16BC_12205"
     CDS             <1..762
                     /locus_tag="AC16BC_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HIR3242421.1"
                     /translation="IFVLIVASFAMAVLHGAMRYGIEDVLVFLALFSGGGFAVENVSV
                     AAGPPFGQYHFTNAFHMPMIGRVPLDVGVMYFAMGYHAWVIGNILLDTADARLAHRFN
                     LIALPVVAAFVMVMWGVVVDPGSATILQVWIWHNSGGYFGVPLINYLGWYAVVYAFFQ
                     GFAWYLAARTGRAPLQVHHLGARYWLPPVVLYLLVAAGALALYVVSPDTEHADALGYV
                     WRSQDIYETAAIISIFTMGFAGVLAVVRLFQPRRD"
     gene            complement(951..1454)
                     /locus_tag="AC16BC_12210"
     CDS             complement(951..1454)
                     /locus_tag="AC16BC_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242422.1"
                     /translation="MNRTGPLHADRGIGCRLFAIAWLALTVTSAVSCRSGDDSPDAKI
                     RATIARAETFAEAKDLGGLRDLLSDAYRDDAGQDKRAILGLLFLQVRRHESIHLLTRI
                     DRIDVRPPQAESTVFVAMAGSPIAGADDLPSLRADLYRFDLTLVEERGTWRVAKSSWR
                     PAALTDF"
     gene            1536..1865
                     /locus_tag="AC16BC_12215"
     CDS             1536..1865
                     /locus_tag="AC16BC_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242423.1"
                     /translation="MRFSTAFRVSGVFVLALAAEVLAAYFLYSNYAYDLANSGDYTVQ
                     EAYASGPIGPVVKVFYVLFAVTAAAALIPPVIAAVSFAFRRHAPQQPSANETPRMMPP
                     PRPGDWR"
     gene            1966..>2145
                     /locus_tag="AC16BC_12220"
     CDS             1966..>2145
                     /locus_tag="AC16BC_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242424.1"
                     /translation="MGVVLLSRWRVGVSQRCETLTRRGYLTTGGWSMNKRMVLVGLAL
                     AAVVGVVGAPVFQSVA"
BASE COUNT          322 a          643 c          740 g          440 t
ORIGIN      
        1 atcttcgtgc tgatcgtcgc gagcttcgcg atggccgtcc ttcatggcgc gatgcgttac
       61 ggcatcgagg acgtgctggt gttcctcgcg ctgttctcgg ggggcggttt cgcggtggag
      121 aacgtcagcg tggccgccgg gcctcccttc ggccaatacc acttcaccaa cgcctttcac
      181 atgccgatga tcggtcgcgt gccgctcgac gtcggtgtca tgtatttcgc gatgggctat
      241 cacgcgtggg tgatcggcaa cattctgctg gatacggccg atgcgcggtt ggcccaccgg
      301 ttcaatctga tcgcccttcc cgtggttgcg gcgttcgtga tggtgatgtg gggcgtggtg
      361 gtggatcccg gcagcgccac gatcctgcag gtctggatct ggcacaacag cggcggctac
      421 ttcggggtcc cgctgatcaa ctatctcggg tggtacgcgg tcgtgtatgc gttcttccag
      481 ggcttcgcgt ggtacttggc cgcgcgcacc gggcgtgcgc cgctccaggt gcatcatctt
      541 ggcgcacgct actggcttcc gcccgtggtg ctgtacctgc tcgtggccgc gggcgcgttg
      601 gcgctgtacg tcgtgtctcc ggacaccgag catgccgacg cgttgggcta cgtgtggcgc
      661 agccaggaca tttacgaaac cgccgccatc atcagcatct tcaccatggg attcgcaggc
      721 gtcctggccg tcgtgcgact cttccagcca cggcgcgact agggtgtgtg aacctgatca
      781 cgggcctgat atcgagatcc cttgggacaa ggagactcgg tagaacctgt gggctgctca
      841 aaaagcccca gctgcaaggc cgctgggagg aggaacccgg agcgtacggg gtcgtacgtg
      901 aggagttccg acgaccgaga aggccgcaga tgggtttttt cagcagcctg ctaaaagtcg
      961 gtgagtgcgg ccggtcgcca tgacgatttg gcgacgcgcc aggttccgcg ttcctcaacc
     1021 aacgtgaggt cgaatcgata cagatcggcg cgcagggagg ggagatcgtc ggcgccggca
     1081 atgggactgc ccgccatggc gacaaacacc gtggactcgg cttgtggcgg gcgcacatcg
     1141 atgcggtcga tgcgggtcaa gaggtggatg gattcgtggc ggcggacctg caagaagagc
     1201 agtccgagga tcgcgcgttt gtcctgtccc gcgtcgtcgc gatacgcgtc ggagagcagg
     1261 tccctcagtc cgcccaggtc tttggcttcc gcgaacgtct ccgcgcgcgc aatcgtggcg
     1321 cgaatcttcg cgtccgggga gtcgtctccg gatcggcagg acacggcgct cgtgaccgtc
     1381 agcgccagcc atgcgatcgc gaaaagccgg cacccgatgc cgcggtcggc gtggagaggt
     1441 ccggtccggt tcatcatcga ccagtgtagc gcaggccggg cggtctggcc gctttctttc
     1501 ccgatttgcc gtggcgtatc atgggggaat cccccatgag gttctcgacc gcattcaggg
     1561 tgtccggggt cttcgtgctc gccttggcgg ccgaagtcct ggccgcgtat ttcctgtact
     1621 ccaactatgc ctatgacttg gcgaattccg gtgactacac cgtgcaggaa gcgtatgcct
     1681 cggggcccat cgggccggtg gtgaaggtgt tttatgtgtt gttcgcggtg accgcggccg
     1741 ccgcgttgat cccgccggtg atcgccgcgg tgtcgttcgc gttcaggcga cacgccccgc
     1801 agcagccgag tgcgaacgag accccgcgga tgatgccccc gcctcgtccg ggagattggc
     1861 gctgacgacc cggggccgag gcgatggcgt cgagccggtg aataattggt cgccgtcttc
     1921 gtcgatcttc cggggagcga cgcctgaaac gaccttgaca gtgcgatggg cgtcgtctta
     1981 ctctcgcgct ggcgcgtcgg ggtatcacag cgatgtgaaa cgctgacccg gcgcgggtat
     2041 ctcacaacag gaggttggtc catgaacaag cggatggttc tggtgggact cgccttggcg
     2101 gcggtcgtcg gcgtggtcgg cgcacccgtc ttccagtcgg tcgcg
//
LOCUS       DBNGTT010000528         4593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_765133, whole genome shotgun sequence.
ACCESSION   DBNGTT010000528 DBNGTT010000000
VERSION     DBNGTT010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_765133"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2173)
                     /gene="rpoB"
                     /locus_tag="AC16BC_12225"
     CDS             complement(<1..2173)
                     /gene="rpoB"
                     /locus_tag="AC16BC_12225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3242425.1"
                     /translation="MASTTEGVRERKDFSKIPASAEIPNLIDIQRRSYDRFLQRDARP
                     EAREDIGLQAAFTSVFPIVDYNETAMIEFLDYTVGAPKYDVRECLERGMTFAAPLKIR
                     ARLIVWDKDEKAGTKKVRDVREQEVYVGELPLMTENGTFIINGTERVVVSQLQRSPGV
                     IFTHDKGKTHASGKVLYSARVIPYRGSWLDFEFDAKDILYVRIDRRRKIPATVLLKAF
                     GYSVEDLLKTYYPIEEIEVSNGHFVRKLDAEIHHGIKASVDLVDKKAKEVIVKEGAKL
                     TRATLKKLKSVGVKEMPISKTDLVGRAVFNDIVDPTTGEVLVERNQELTEAIIEKIAT
                     GAIEALQLLYIDNVQVLPVIRDTMALEKVGSPEEAMVEIYRRLRPGETPTVDTARSLF
                     DNLFFNSKRYDLSPVGRLKLNKKLGLDVPLDKRTLTAQDIVEAVRYLIGLKTGKGDID
                     DIDHLGNRRVRSVGELLENQLRIGLVRMERTIKERMNLLDIETVLPHDLINAKPVIAV
                     IKEFFGSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYGRIC
                     PIETPEGPNIGLITSLATYARVNEFGFIESPYRKVESGRVTDHVEFISAIDGDKYSVA
                     QANTKVDGRGKITLDSVSARAAGDFITLTPEKIEYMDVSPKQIVSVATALIPFLENDD
                     ANRALMGSNMQRQAVPLLTSDPPLVMTGMEEVVARNSSMVVK"
     gene            complement(2252..2632)
                     /gene="rplL"
                     /locus_tag="AC16BC_12230"
     CDS             complement(2252..2632)
                     /gene="rplL"
                     /locus_tag="AC16BC_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3242426.1"
                     /translation="MTNDEILKAVEAMPVLQLSELVKALEEKFGVTAAAPVAMMAAPG
                     GAAAAAAPAEEKTAFDVILAAVPADKKIQVIKVVRELTNLGLKEAKDLVEGVPKPVKA
                     GVAKEESDTMKKKLEEVGAKVEIK"
     gene            complement(2739..3269)
                     /gene="rplJ"
                     /locus_tag="AC16BC_12235"
     CDS             complement(2739..3269)
                     /gene="rplJ"
                     /locus_tag="AC16BC_12235"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3242427.1"
                     /translation="MKGKAEKAVLIEDIHGRFAKAQMAVVAEYRGMPVEEVTVLRGKL
                     RAAGGEFKVVKNTLAKRAAEGTALVGLREYFDGPVGVALGYQDPAPLAKALKDFAGQQ
                     EKFKLRVGVLEGRVLDLAGLTRVASLPSRQVLLGRLVGQMQAPVSGLVGGLQGVIRKA
                     VSTLDAVAKKRMAEAG"
     gene            complement(3567..4259)
                     /gene="rplA"
                     /locus_tag="AC16BC_12240"
     CDS             complement(3567..4259)
                     /gene="rplA"
                     /locus_tag="AC16BC_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3242428.1"
                     /translation="MGKKFEAAKQRVQAETYSLREALELAKATSFVKFDASVDLAVRL
                     GVDPKHSDQMVRGSVVLPHGAGKTVTIAVFAKGDKEREAREAGADHVGAEELAEKISK
                     GWMEFDRVVATPDLMGVVGRLGKILGPRGLMPNPKTGTVTFDVARAIREIRQGKVEYK
                     VEKAGIVHVPVGKASFDVAKLHDNALSILDSIIKAKPAAAKGTYVKSVSVSATMGPGI
                     KIDPASIARPVA"
     gene            complement(4277..>4593)
                     /gene="rplK"
                     /locus_tag="AC16BC_12245"
     CDS             complement(4277..>4593)
                     /gene="rplK"
                     /locus_tag="AC16BC_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR3242429.1"
                     /translation="FCKTFNAKTQQLGDSIIPVVITVYADRTFTFITKTPPASDLLKK
                     AAGVVKGSAVPQKDKVGKLTAAQIQEIAKTKMPDLNAVDLAGAIKIIEGTARSMGLQI
                     VK"
BASE COUNT          755 a         1580 c         1292 g          966 t
ORIGIN      
        1 ccttgacgac catgctgctg ttcctggcga cgacctcctc catgccggtc atgaccagcg
       61 gcgggtcgct cgtcagcaac ggcacggcct gccgctgcat gttcgaaccc atcagcgcgc
      121 ggttggcgtc gtcgttttcc agaaatggga tcagcgccgt cgccacgctc acgatctgct
      181 tgggcgacac gtccatgtac tcgatctttt ccggcgtcag cgtgatgaag tccccggccg
      241 cccgcgcgga cacgctgtcc agcgtaatct tgccccgccc gtccaccttg gtgttggcct
      301 gcgccaccga gtacttgtcg ccgtcgatgg ccgaaatgaa ctccacgtgg tcggtcaccc
      361 ggccgctctc aaccttccga tagggggact cgatgaagcc gaactcgttg acccgggcgt
      421 acgtggccaa cgaggtgatc aacccgatat tggggccttc cggagtctcg atcgggcaga
      481 tccggccgta gtgcgacgga tgcacgtcgc gcacctcgaa acccgcgcgc tcgcgcgtca
      541 acccgccggg ccccagcgcg gacagccggc gcttgtgcgt gatctcggcc aaggggttgg
      601 tctgatccat gaactgcgag agctggctcg acccgaaaaa ttccttgatc accgcgatca
      661 ccggcttcgc gttgatgagg tcgtgcggca acacggtctc gatgtccagg agattcatac
      721 gctccttgat ggtccgctcc atccgaacca gaccgatccg gagctgattc tccaacagtt
      781 cgccgaccga gcgcacgcgc cgatttccga ggtgatcgat gtcgtcgatg tcgcccttgc
      841 cggtcttcag gccgatcaga taccgcacgg cttccacgat gtcctgcgcc gtgagcgtgc
      901 gcttgtcgag cggcacgtcg agtcccagct ttttattgag tttgaggcgt ccgaccggcg
      961 agaggtcgta ccgcttggaa ttgaagaaca ggttgtcgaa gagcgaccgc gcagtgtcca
     1021 ccgtgggcgt ttccccggga cgcagacggc gatagatctc caccatcgcc tcctcgggcg
     1081 agcccacctt ttccaaggcc atggtgtcgc gaatcaccgg caacacctgc acgttgtcga
     1141 tatacaacaa ctgcagggcc tcgatcgcgc cggtcgcgat cttctcgatg atcgcttcgg
     1201 tcagttcctg attgcgctcg accaacacct cgccggtggt cggatccacg atgtcgttga
     1261 acaccgcgcg ccccaccagg tcggtctttg aaatcggcat ctctttgacc ccgacgctct
     1321 tgagcttctt gagcgtggcg cgggtcagct tggcgccttc cttgacgatg acttccttgg
     1381 ccttcttgtc gaccaggtcg acggaggcct tgatcccgtg atggatctcc gcgtccagct
     1441 tccggacaaa gtgcccgttg gacacttcga tctcctcgat cgggtagtac gtcttgagca
     1501 ggtcttcgac cgagtacccg aaggccttga gcaggaccgt ggccggaatc ttccggcggc
     1561 ggtcgatgcg cacgtacaag atgtccttgg cgtcgaactc aaagtcgagc cacgaaccgc
     1621 gataggggat cacccgcgcg gagtacagca ccttgccgct cgcgtgcgtc ttgcccttgt
     1681 cgtgcgtgaa aatgacgccg ggcgagcgct gcaactggct gaccaccacg cgttccgtgc
     1741 cgttgatgat aaaggtgccg ttttcggtca tcagcggcag ttcgccgacg tacacctcct
     1801 gctctcgcac gtcgcggacc ttcttggtcc cggctttctc gtccttgtcc cacacgatca
     1861 gacgcgcacg gatcttgagc ggcgcggcga acgtcatgcc gcgctccaga cactcgcgca
     1921 cgtcgtactt gggcgctccc accgtgtaat cgaggaattc gatcatcgcg gtttcgttgt
     1981 aatccacgat ggggaacacc gacgtaaagg ccgcctgcaa gccgatatcc tcgcgcgcct
     2041 ccggcctggc atctcgttgc agaaaccggt cgtaagaccg ccgctggatg tcgatcaggt
     2101 ttggaatttc cgcgctggcc gggatcttgg agaaatcctt gcgttccctc acgccttcag
     2161 tcgtcgacgc cattcatgct cctcgttatg tcggggcgtt ctccacgaac gccccgacca
     2221 gactcatcag gacttgacac tggtacccgc gttacttgat ttcgaccttt gcgccgactt
     2281 cttccaactt cttcttcatg gtgtcggatt cttccttggc cacgcccgcc ttgaccggct
     2341 tcggcacacc ttcgaccaag tccttggcct ccttcaggcc caggttcgtc agttcgcgga
     2401 ccaccttgat gacctggatc ttcttgtcgg ccggcaccgc cgcgagaatc acgtcgaacg
     2461 cggtcttttc ctccgcggga gcagcagccg ccgccgcgcc gccgggcgcg gccatcatgg
     2521 ccaccggcgc cgcagcggtc acgccgaact tctcctccaa cgccttgacc aactcggaaa
     2581 gctggagcac cggcatcgcc tcaactgcct tcagaatctc gtcattcgtc aacgtcgcca
     2641 tctcacatct cctctagata gatttcgtta gtttcgtcga ttcgttgtgt cgtccatggc
     2701 gggcggccac aagagagagc cggccccgac acgaaaattc atcccgcctc ggccatgcgt
     2761 ttcttggcca cggcatccag ggttgaaacc gctttccgga tcacgccctg caaaccaccc
     2821 acgagccccg acaccggcgc ctgcatctga ccgaccaatc ggccgagcaa cacctggcgc
     2881 gacgggagcg acgccacccg cgtcaggccc gccaagtcga gcacgcgtcc ttccagcacg
     2941 ccgacgcgca acttgaactt ctcctgctgg cccgcgaagt ccttgagcgc tttggccaac
     3001 ggcgccggat cctgataccc caacgccacg ccgaccggcc cgtcgaaata ctcccggagt
     3061 ccgaccagcg ccgtgccctc ggccgcgcgt ttggccagcg tgttcttgac caccttgaac
     3121 tcgccgccgg ccgcgcgcag cttgccgcgc aggacggtga cctcttcgac cggcatccct
     3181 cggtattcgg ccaccaccgc catctgagcc ttggcgaacc gtccgtgaat gtcctcgatc
     3241 aataccgcct tttccgcttt ccccttcacc gcctcacctc ccccctttcg tgttcacgga
     3301 cggcctggct tcggaacgcc tccgagacga aaggggtgtc aaccaaccgg catcaccgat
     3361 catggcgtat gcccacgatc ggtgatttgt gattctgccc cgcgttcatc tcggtaggac
     3421 gcccggagcg acccgctccg tcgtttatgc caatcggctc ctactgtctc ggacgaaccc
     3481 cgccaggtgc cgctcacggc ggagcctgac gagcccactg tgtgttcccc ttgccttgcg
     3541 actctctccg cctaacgcga tcgagcctac gccaccggtc gcgcaatgct ggccgggtcg
     3601 atcttgatgc cgggtcccat ggtcgcggac acggacaccg acttcacgta tgttcccttg
     3661 gccgccgcgg gtttggcctt gatgatggaa tccaggatgg acaaggcgtt gtcgtggagt
     3721 ttggccacgt cgaacgaggc cttgcccacg ggcacgtgaa cgatccccgc cttctccacc
     3781 ttgtactcga ccttcccctg acggatctcg cgaatcgcgc gggccacgtc gaaggtcacg
     3841 gtgccggtct tgggattcgg catcaggccg cgcgggccga ggattttgcc caatcggccg
     3901 accaccccca tcaagtccgg ggtcgccacc acgcgatcga actccatcca gcccttggag
     3961 atcttctcgg cgagttcctc ggctccgacg tgatccgctc cggcttcgcg ggcttcgcgt
     4021 tccttgtcgc ccttcgcgaa caccgcgatg gtcacggtct tcccggcgcc gtgcggcaac
     4081 acgaccgatc cgcggaccat ctggtccgag tgcttcggat ccacgcccag ccggaccgcc
     4141 aggtcgaccg acgcatcgaa tttcacgaaa ctcgtggcct tcgctaattc cagggcctcg
     4201 cgcaacgaat acgtctccgc ttggacccgc tgtttggccg cttcaaattt ctttcccatt
     4261 gctcgcttcc tttcggctac ttgacgatct gcaggcccat gctccgagcc gtgccttcga
     4321 tgatcttgat cgcgccggcg aggtccacgg cgttcagatc gggcattttg gttttggcga
     4381 tttcctggat ctgcgccgcc gtgagtttac cgaccttgtc cttctggggc acggccgacc
     4441 ctttgaccac ccccgccgcc ttcttgagca gatccgaggc cggaggggtc ttggtgatga
     4501 acgtaaaggt gcggtcggcg taaaccgtga tcaccaccgg gataatggag tcgccgagct
     4561 gctgcgtttt ggcgttgaag gtcttgcaga act
//
LOCUS       DBNGTT010000529         4622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2297438, whole genome shotgun sequence.
ACCESSION   DBNGTT010000529 DBNGTT010000000
VERSION     DBNGTT010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2297438"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="AC16BC_12250"
     CDS             <1..585
                     /locus_tag="AC16BC_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3242430.1"
                     /translation="LRQDPDVILVGEMRDFETISTALVAAETGHLVMSTLHTVDATET
                     INRIISVFPPYQQKQVRLQLAGVLKGVISQRLVPRIDGQGRVPAVEVMVATQTIRECV
                     IDPDKTRRIHDVIGAGTSQYGMQTFDQSLAQLCRDKLVAYEEALRWSSNPDDFALKMK
                     GVQSTGDLTWETQKPAPAPAAAPDPGFKIERFGS"
     gene            582..3233
                     /gene="alaS"
                     /locus_tag="AC16BC_12255"
     CDS             582..3233
                     /gene="alaS"
                     /locus_tag="AC16BC_12255"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR3242431.1"
                     /translation="MNAHDIRRAFCDFFVAKHGHTLVPSSSLIPAQDPTLLFTNAGMV
                     QFKQTFLGDEPRPYARAVSVQKCVRAGGKHNDLEHVGYTGRHHTFFEMLGNFSFGDYF
                     KDGAIRMAWEFVTEVLKLPPARLWVTVFREDDEAARLWREAVGVRPDRIVRLGEKDNF
                     WQMGDTGPCGPCSEIYVDQGERVGCGKPTCGVGCDCDRYLEIWNLVFMQYNRDASGTL
                     SPLPRPSIDTGMGLERVAAVTQGALSNYDSDLFRPLLDAIGRACGKVYGRDAASDVSM
                     RVIADHLRALTFLLSDGVLPSNEGRGYVVRRILRRAARHGKLLGFDGPFLHELSGTVA
                     AQMQDAYPEVGRNRQAVARTLLAEEERFAQTLSHGMTILSGLVDHAKQSKASALTGPD
                     LFKLYDTYGFPMDLIADVAKEHGLALDEAGFAAAMEEQRERARAAGGFEAEQVQTIYK
                     DTAAQTGPTTFVGEEELEADVRVLAILKDGARVREAREGEQVELMFDHTPCYGEGGGQ
                     LGDQGELVGPAADAQIRDTIIPVKGLYVHAVTVRRGRIVEGERYRVSVNHAARQACSN
                     HHTGTHLLHAILRDVLGDHVKQAGSLVAPDRLRFDFHHFAPLSPRELERVEAEVNRRI
                     QEDHHVDKAWMSQRDALASGALAFFGEKYGDRVRVVSIGSFSKELCGGTHTRDTGELG
                     LLKIVREAGVAAGVRRIEAVAGEPAYLAARKDSQDLADIAAVLKVQPAEVVSKARKLV
                     ESMRTREREIEQLKARLAGGGRSEAGSDRGRTVAGVTVAVRRIDGMDAKDLRAAADRV
                     REQIKSGVVVLGAVAGDKVNLVAAVTPDLSGRFHAGNLVRAVADLVGGTGGGNATMGQ
                     AGGRQPERLDEVLERVDALVEGMASKS"
     gene            3265..3750
                     /gene="ruvX"
                     /locus_tag="AC16BC_12260"
     CDS             3265..3750
                     /gene="ruvX"
                     /locus_tag="AC16BC_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR3242432.1"
                     /translation="MPELVSESIPETARPRVRRMLALDVGDRRIGVAVSDELGITAQG
                     VTTIHRRSWAADLAEIARLVEHWDAETILVGWPLTLQGTVGPRARIVQDFIARLGKMV
                     RVPITTWDERFTTATAERVLIDADVSRARRRQVVDKAAATIILQDFLDARANREAEGR
                     S"
     gene            3779..>4622
                     /gene="mltG"
                     /locus_tag="AC16BC_12265"
     CDS             3779..>4622
                     /gene="mltG"
                     /locus_tag="AC16BC_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3242433.1"
                     /translation="MSRQRVAPPEYAWVLGLLIMLGMLACGVHVASFRASPSGKDGGP
                     AATFEIAHDESLTHVASRLASDGLIRNQTYFVIFGRLTGSERQLKPGEYALSPSMRPL
                     EILDRFRRGLVVLHPVTIPEGYSVRQIAEVFREARIGDPEELISLSESPAFIRELGLD
                     VPTLEGYLFPDTYAFPRHITTADIVRQMVRRFQETYQSVWAARADGLGLTQHQVVTLA
                     SIIEKETGAGIERPIISAVFHNRLRLRMPLQSDPTVIYPIKDFDGNLRKVDLIRDNPY
                     NTYRR"
BASE COUNT          774 a         1428 c         1663 g          757 t
ORIGIN      
        1 ttgcgccagg accccgacgt gattctggtc ggcgagatgc gcgacttcga gacgatttcc
       61 accgcattgg tggcggcgga aaccgggcat ctggtcatga gcaccctgca caccgtggac
      121 gcgaccgaga ccatcaaccg catcatctcg gtgtttcccc cgtaccagca gaagcaagtc
      181 cgcctgcagc tcgccggggt cctcaagggc gtgatctccc agcgcctggt gccgcggatc
      241 gacgggcagg gccgcgtgcc cgcggtggaa gtcatggtcg cgacccagac gatccgcgag
      301 tgcgtgatcg accccgataa gacccgccgg attcacgacg tgatcggggc gggcacgtcc
      361 cagtacggga tgcagacgtt cgaccagtcg ctggcgcagt tgtgccgcga caaactggtc
      421 gcgtatgaag aggccctgcg ctggtcgtcg aacccggacg acttcgccct gaagatgaag
      481 ggggtgcagt ccaccggcga tctcacgtgg gagacccaga agcccgcgcc ggctccggcc
      541 gccgccccgg accccgggtt caagatcgaa cggttcggct cgtgaatgcg cacgacatcc
      601 gccgcgcgtt ctgcgacttc ttcgtcgcga agcacggcca caccctggtt cccagttcgt
      661 cgctgatccc ggcgcaggac cccacgctcc tgttcaccaa cgcgggcatg gtgcagttca
      721 agcagacctt tctgggggac gagccccggc cgtacgcgcg cgcggtgtcg gtgcagaagt
      781 gcgtccgggc cggcgggaaa cacaacgacc tcgaacacgt gggatacacc ggacggcacc
      841 acacgttttt cgagatgctc gggaactttt ccttcggcga ctatttcaag gacggcgcga
      901 tccggatggc gtgggagttc gtgaccgagg tgctcaaact gccgcccgcc cgactctggg
      961 tgaccgtgtt tcgcgaggac gacgaagcgg cccggttgtg gcgcgaggcc gtgggcgtgc
     1021 ggcccgatcg catcgtgcga ttgggcgaaa aggacaactt ctggcagatg ggcgacaccg
     1081 ggccgtgcgg gccctgctcg gagatctacg tggatcaagg cgaacgggtc ggctgcggca
     1141 aacccacgtg cggggtgggc tgcgactgcg accgctacct cgagatctgg aacctggtct
     1201 tcatgcagta caaccgcgac gcctccggca ccttgtcccc gcttccccgc ccgagtatcg
     1261 acaccgggat gggattggaa cgtgtggccg cggtgacgca gggcgcgctc agcaactacg
     1321 acagcgacct gttccgtccg ttgctggacg cgatcgggcg ggcgtgcggg aaggtgtacg
     1381 gacgcgacgc cgcgtccgac gtgtccatgc gggtgatcgc cgatcacctc cgcgcgctga
     1441 cgtttttgct ctccgacggc gtgttgccct ccaacgaggg ccgcgggtac gtggtccgtc
     1501 ggatcctgcg ccgggccgcg cgtcacggca agctgctggg tttcgacggt ccgttccttc
     1561 acgagctgtc gggcacggtg gcggcccaga tgcaggacgc gtatccggag gtgggtcgga
     1621 atcggcaggc ggtggcgcgc acgttgctcg cggaggaaga gcgcttcgcg cagaccctca
     1681 gccacggcat gaccatcctc tcggggttgg tggatcacgc gaagcagtcc aaggcctccg
     1741 cgctgaccgg gcccgatctg ttcaagttgt acgacaccta cgggtttccg atggatctga
     1801 tcgcggacgt ggccaaggaa cacgggctcg cgctggacga ggccggattc gccgcggcca
     1861 tggaggagca gcgcgagcgc gcccgcgccg cgggcgggtt cgaagccgag caggtgcaga
     1921 cgatttataa ggacaccgcg gcgcagaccg gccccaccac gttcgtgggc gaggaggagt
     1981 tggaggccga tgtccgggtg ctcgcgattc tcaaggacgg cgcccgcgtg cgcgaagcgc
     2041 gcgaaggcga gcaggtcgaa ctgatgttcg atcacacgcc gtgctacggc gagggcggcg
     2101 gccagctcgg ggaccagggc gaactggtcg ggcccgcggc ggacgcgcag attcgggaca
     2161 cgatcatccc ggtcaagggc ctgtacgtgc acgcggtcac ggtccgtcgc ggccggatcg
     2221 tcgagggcga gcggtatcgg gtcagcgtga accacgcggc gcggcaagcc tgttcgaacc
     2281 accacacggg cacgcacttg ctgcacgcga tcctgcgcga cgtgctggga gaccacgtga
     2341 aacaggccgg gtcattggtg gcgccggatc gattgcgttt cgactttcac catttcgccc
     2401 cgttgtcgcc gcgggagttg gagcgggtcg aagcggaggt caatcggcgc atccaggaag
     2461 accaccacgt ggacaaggcg tggatgtcgc agcgcgacgc gttggcgagc ggggccttgg
     2521 cgttcttcgg cgagaaatac ggggaccgcg tccgggtcgt ctcgatcggc tccttcagca
     2581 aggaattgtg cggcgggacc catacccgtg acaccggcga gttggggctc ttgaagatcg
     2641 tgcgcgaggc gggggtggcg gcgggtgtgc gccgcatcga agcggtcgcg ggcgagccgg
     2701 cgtacctggc ggccaggaag gattcgcagg atctggcgga catcgccgcc gtgctcaagg
     2761 tccagccggc ggaagtggtt tccaaggccc gcaagctggt cgagtcgatg cgcacgcggg
     2821 agcgggagat cgagcagctc aaggcgcgcc tggccggcgg cgggcggagc gaagccgggt
     2881 cggaccgcgg tcgaaccgtg gccggcgtca ccgtggcggt ccggcggatc gacggcatgg
     2941 acgccaagga cctgcgggcc gcggccgatc gcgttcgcga acagattaag tccggcgtcg
     3001 tggtgcttgg cgcggtcgcc ggggacaagg tcaatctggt cgcggcggtc acgccggacc
     3061 tgtccggccg cttccacgcc ggcaacctgg tcagagccgt ggcggatctt gtcggcggca
     3121 ccggaggcgg gaacgcgacg atgggccagg ccggcggacg gcaacccgaa cggctggacg
     3181 aggtgctgga gcgggtggat gccctggtcg aagggatggc gagcaaatca tgagcgcggg
     3241 ctccccccgt atggatgcgc ggagatgcca gaactcgtga gcgagtccat ccccgagacc
     3301 gcgcgtccgc gggttcggcg gatgttggcg ctggacgtgg gcgatcggag gatcggcgtg
     3361 gcggtgagcg acgagttggg catcaccgcg caaggagtga ccaccattca ccgccggtcg
     3421 tgggccgcgg atctggccga gatcgcccgg ctggtcgagc actgggacgc ggaaacgatc
     3481 ctggtcgggt ggcccctcac gctacagggc accgtggggc cccgcgccag aatcgtgcag
     3541 gacttcatcg cgcgattggg gaaaatggtg cgcgtcccga ttaccacgtg ggatgagcgg
     3601 ttcaccacgg cgaccgccga acgcgtattg atcgacgcgg acgtgtcgcg cgcccggcgc
     3661 cgccaagtgg tggacaaggc cgcggccacg atcattctcc aggactttct tgatgcgcgg
     3721 gcgaatcggg aagcggaggg tcgttcgtga cggccgccgc cgctgaaagg gacgatcgtt
     3781 gagccggcaa cgcgtagccc caccggaata tgcgtgggtc ttgggcctgc tcatcatgtt
     3841 gggcatgctg gcgtgcggcg tccacgtggc ctcgtttcgg gcctcgccat caggcaaaga
     3901 cggcgggccc gccgcgacgt tcgagatcgc ccacgacgaa tcattgaccc atgtggcgtc
     3961 gcgcctggca tccgacggat tgatccgcaa tcagacgtat ttcgtgatct ttggacgcct
     4021 gaccgggagc gagcgacagc tcaaaccggg tgagtacgcg ctctccccgt cgatgcgtcc
     4081 gttggagatc ctcgaccggt ttcgccgcgg cctcgtggtg ctgcatccgg tgaccattcc
     4141 ggaggggtac tccgtgcgcc agatcgccga ggtgtttcgg gaggcgcgta tcggcgatcc
     4201 ggaggaactg atctcgctga gcgagtcccc cgcgttcatt cgggaactcg ggttggacgt
     4261 gccgaccctg gaagggtacc tgtttccgga cacgtatgcg tttccgcgcc acattaccac
     4321 cgcggacatt gttcgtcaga tggtacggcg gtttcaggag acgtatcagt ccgtgtgggc
     4381 ggcgcgagcg gacgggttgg ggttgacgca gcaccaggtg gtcacgctgg cgtcgatcat
     4441 tgaaaaggaa acgggggccg gcatcgagcg gccgatcatc tcggcggtct ttcacaatcg
     4501 actccgcttg cggatgccgc tgcaaagcga tcccaccgtg atatatccga tcaaggattt
     4561 cgacggcaac ctgcgaaagg tggatctgat tcgcgacaat ccgtacaaca cgtatcgtcg
     4621 cc
//
LOCUS       DBNGTT010000530         6542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2042559, whole genome shotgun sequence.
ACCESSION   DBNGTT010000530 DBNGTT010000000
VERSION     DBNGTT010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2042559"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="tgt"
                     /locus_tag="AC16BC_12270"
     CDS             complement(<1..947)
                     /gene="tgt"
                     /locus_tag="AC16BC_12270"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3242434.1"
                     /translation="MGAQEVHAVADVAVPETTHDAASDRVFTVSAVDPGTGARRGRLR
                     TAHGVVETPAFMPVGTSAAVKGLDPDDLVAVGAHIILANTYHLWLRPGHRVIADLGGL
                     HRFMGWSRPILTDSGGFQVYSLAALRRVSDEGVSFRSHLDGSLRHLTPEGAIEIQDAL
                     GADIVMAFDECLAYPATEAEAAEALRRTVAWARRCAEVRRSRPSLLFGIVQGGFSLPL
                     RDRAVEETVAVGFDGYAIGGLSVGEPKDEMLRVIDHVAPRLPASRPRYLMGVGTPADL
                     VDGVARGLDLFDCVMPTRHARTGWLFTSEGRVSIKHARYT"
     gene            complement(947..2626)
                     /gene="argS"
                     /locus_tag="AC16BC_12275"
     CDS             complement(947..2626)
                     /gene="argS"
                     /locus_tag="AC16BC_12275"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR3242435.1"
                     /translation="MPVTVANSIKRRVTEILDQALHRARSVGELRSAPTAVSVELPKR
                     AGRGDYATTVALTLAPIERRPPGHIAEIVKRHIETTDLVESVEIAGPGYVNLTVRPEA
                     WRAVILQVLGAGAEYGRGEASGRSVQVEFVSANPTGPLHVGHGRLAAVGLALANLLAA
                     AGDRVEREYYINDAGRQMRLLGESVWARYCTLAGRSTPVPEDGYQGAYIEEIARGIVG
                     DVGPRYLDAPREEALAAVTAAAYTQLLEEIRVDLDAFGVAFDAWTSERALFASGAVEQ
                     VIDRLRSLGYMYEADGALWFQASALGDEKDRVVRKQDGEWTYLASDLAYHLQKLTRGF
                     DVVIDLWGADHHGYIGRIRALFRALGYPDERLRVEIGQLVTVVRKGKPVPMSKRAGTF
                     VTLRDVIDEVGRDAALLTFQMRRLDSPMEFDLELAKEQSSENPVYYIQYAHARVASIL
                     RAAAEQSLSWQSAGEAELARLELPEELGLLKALAAYPDVIADAAAAYEPHRLAYFLQE
                     LAGQFHGYYYKHRIIGDDRTVSLARVALAAAVGVVIRNGLTIFGVTAPDRM"
     gene            complement(2779..3228)
                     /locus_tag="AC16BC_12280"
     CDS             complement(2779..3228)
                     /locus_tag="AC16BC_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HIR3242436.1"
                     /translation="MNERSFPPHSTLPAWWAPSRPTDHAVGASDRMIVLLYSEALTRL
                     RRARTSPHGADRHVHTVLAILAELSNALASREDQEAVVDLLSLYRYMSHRLTSASQRV
                     PVEALAEVEHLFDTLFDGWIQVLQGEWQAGFPDCLNTDDSRATPTTL"
     gene            complement(3225..3686)
                     /gene="fliW"
                     /locus_tag="AC16BC_12285"
     CDS             complement(3225..3686)
                     /gene="fliW"
                     /locus_tag="AC16BC_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HIR3242437.1"
                     /translation="MKIETRRFGTVEIDDGAIIVLPKGLIGLPLLTRFIALGHERAGC
                     FSWWQSVDDRDTALVAVDPSSILPDYDLKGLEADVADLGLEDARACQVAVFATISGPT
                     LDSVALNLAAPVIVNPATRRGRQVVFQDGRYPVDARWDDLLSPKRSFELAA"
     gene            complement(3683..3943)
                     /gene="csrA"
                     /locus_tag="AC16BC_12290"
     CDS             complement(3683..3943)
                     /gene="csrA"
                     /locus_tag="AC16BC_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR3242438.1"
                     /translation="MLVLTRRVEEGIMIGDDIRVRVLEVKGHQVKLGIEAPRSTGVYR
                     EEIYQRIQEENRRASQVPEPSGVLSSLWREWRNRAGDEGKKP"
     gene            complement(4003..5046)
                     /locus_tag="AC16BC_12295"
     CDS             complement(4003..5046)
                     /locus_tag="AC16BC_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3242439.1"
                     /translation="MSNRLLIVDDEPFVLSALTRLLRRDGYEVITANAPSRGLAILQE
                     QSVGVIISDYRMPEMDGVQLLRRAREVAPSAIRIILSGYAETHAILSAVNDGGIHKYL
                     TKPWNDEQIRLEIKSGFELYRLAVTNRELQETLTRQNEELRTLTKSLAVEQDRQREIF
                     RTTIEMLVSLPKLNSPKEVRQSDRVQAICRATGEHLGLTDDELAHLDLAARLHDVGNL
                     AVDIRILLKSGALDPEETRAVQQHAVVPGHLLSGVPGMETVVQLLRSHHENFDGSGYP
                     DRLSGSSIPALSRILRLVDAYEALRSHRPYRESISAGQAITHIEREAGRAFDPVLIEP
                     FKQVIKGVEGIHG"
     gene            complement(5057..5584)
                     /locus_tag="AC16BC_12300"
     CDS             complement(5057..5584)
                     /locus_tag="AC16BC_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3242440.1"
                     /translation="MSDAPTILVVDDEPSVRRSLCRLFARERYRVLEAEGVRDAIKMM
                     AAGQVDLVITDYAMPDGTGLDLLMTLKQRYPHVVRMIITGKADLDTVVAAINEGHVYR
                     FVLKPWDNEDLCLSVRLALDQARLLSKNRQLVGRLEEQETLLRSLAKQYPGITTVEWS
                     ADGSIVLDDSGEEAA"
     gene            complement(5619..>6542)
                     /locus_tag="AC16BC_12305"
     CDS             complement(5619..>6542)
                     /locus_tag="AC16BC_12305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3242441.1"
                     /translation="KATLEGRVKQRTLEIEDALRERERAHVELTRKDDQLIRQEKMAS
                     LGQLAAGVAHEINNPVGFVNSNLQMLQTYLSDITGYVERTSALLARLTEAGLHDSVVG
                     QIQEIVSWKENTQLAQTIEDARQSVVESVEGLNRVKKIVSALRGFSHADQDKPAWSDL
                     NEGLESTINIVWNELKYKATLHRDYGDLPKVWCYPHQLNQVFMNLLVNAAQAIPERGE
                     IWVKTWADTDTVYVDVRDTGAGIPTEHLSKIFDPFFTTKPVGQGTGLGLSIVYGIVER
                     HGGAVRVESRVGDGTTFHLSFPIERAAKAAA"
BASE COUNT         1221 a         2246 c         1877 g         1198 t
ORIGIN      
        1 gtatatcgcg cgtgtttgat cgacacgcgg ccttcgctgg taaagagcca tccggtacgg
       61 gcgtgccgtg tcggcatcac gcagtcgaac agatccagcc cgcgcgcgac gccgtcgacc
      121 aggtcagccg gcgtgccgac ccccatcaag tagcgcgggc gggatgcggg gagccgcggc
      181 gccacgtggt cgatcacgcg cagcatctcg tctttcggtt cccccaccga gaggccgccg
      241 atcgcgtagc cgtcgaaccc caccgccacg gtctcctcga ccgctcgatc gcgcagcggg
      301 agcgaaaacc cgccctgcac gatgccgaac agcagcgacg gccggctccg gcgaacctcg
      361 gcgcaccgcc gcgcccacgc cacggtccgg cgcaatgcct cggcggcttc cgcctcggtc
      421 gcgggatacg cgaggcattc gtcaaacgcc atcacgatgt ccgcgcccag cgcgtcctga
      481 atctcgatcg cgccctccgg ggtcaggtgt cggagcgagc cgtccaggtg cgaacgaaac
      541 gacaccccct cgtccgacac gcgccgcaac gccgccaggc tgtatacctg aaagcccccg
      601 ctgtcggtca ggatcgggcg ggaccacccc atgaaccggt gcaagccgcc caagtcggcg
      661 atcacccgat ggcccggacg cagccacaaa tgataggtgt tcgcgaggat gatgtgcgcg
      721 ccgaccgcga cgaggtcgtc cggatccagc cctttgaccg cggccgacgt gccgaccggc
      781 atgaacgccg gcgtctcgac cacgccgtgc gcggttcgca aacgaccgcg gcgcgcgccc
      841 gtgcccgggt ccacggcgct gaccgtgaag acccggtccg acgccgcgtc atgggtcgtc
      901 tcaggcacgg cgacatcggc cacggcatgc acctcctgcg cgcccatcac atccggtccg
      961 gagcggtcac gccgaaaatg gtcaggccgt tgcggatcac cacgccgacg gccgccgcca
     1021 acgccacgcg ggcgagggac acggttcgat cgtcgccgat gatccggtgt ttgtagtagt
     1081 acccgtggaa ttgtccggcg agctcttgca ggaaatacgc gagacggtgc ggttcgtagg
     1141 cggccgcggc atcggcgatc acgtcgggat acgcggccag cgctttgagc agccccagtt
     1201 cttcgggcag ctccaggcgc gcgagctcgg cctcgcccgc cgattgccac gacaacgact
     1261 gctccgcggc cgcgcgcaga atgctcgcca cccgcgcgtg ggcgtattga atgtagtaca
     1321 ccggattttc cgacgactgc tccttggcca actccaggtc gaactccatg ggactgtcga
     1381 gccggcgcat ttgaaaggtc aacagcgcgg cgtcgcggcc cacctcgtcg atcacgtccc
     1441 gcagggtgac gaacgtgccc gcgcgtttgg acatcggcac gggtttgcct tttctgacca
     1501 cggtgacgag ctggccgatc tcgactcgca ggcgttcatc cggatacccc aacgcgcgaa
     1561 acagcgcgcg gattcggccg atgtacccgt ggtgatccgc gccccagagg tcgatcacca
     1621 cgtcgaagcc gcgggtgagc ttctggaggt ggtacgcgag atccgacgcc aggtacgtcc
     1681 attcgccgtc ctgcttgcgc accacgcgat ctttttcatc gcccaacgcc gacgcctgga
     1741 accacagggc gccgtccgct tcgtacatgt accccaacga ccgcagccgg tcgatcacct
     1801 gctccaccgc gcccgaggcg aacaaggcgc gctcgctggt ccacgcgtcg aacgccacgc
     1861 cgaacgcgtc gagatccacg cggatttcct ccagcagctg tgtgtaggcc gccgcggtca
     1921 cggccgcgag cgcctcctcg cgcggcgcgt ccagataccg cgggccgacg tcgcccacga
     1981 tgccgcgcgc gatctcttcg atataggcgc cctggtaccc gtcctccgga acgggcgtcg
     2041 aacgtcccgc cagcgtgcaa taccgcgccc agacggactc cccgagcagc cgcatctgcc
     2101 gtccggcgtc gttgatgtaa tactcgcgct cgacccgatc ccccgcggcc gccagcagat
     2161 tggccaacgc cagccccacg gccgcgagcc ggccgtgccc gacgtgcaac gggccggtgg
     2221 gattggcgct cacgaactcc acttggacgg accgcccgga cgcctccccg cgaccgtact
     2281 cggcaccggc accgagcacc tgcaggatca cggcgcgcca ggcctcgggg cgaacggtca
     2341 gattcacgta gcccggcccg gcaatctcca cggactccac caagtccgtg gtctcgatat
     2401 gccgcttgac gatttccgcg atatggccgg gcggtcgccg ttcaatgggc gccaaggtca
     2461 acgcgaccgt ggtggcgtag tcgccgcgcc ccgcgcgctt ggggagttcc accgagaccg
     2521 ccgtcggggc gctgcgcagc tctcccacgg atcgggctcg gtgcagcgct tgatccagga
     2581 tctccgtgac tcgccttttg attgaattgg ccaccgttac aggcataagc gtagccccct
     2641 agtataggga tcaaggatca aaagtcaatg aattccgccg actgtgaagt ggccgcgggg
     2701 acgacggcct cacggtgatg tcgagcacgg tcgggccgaa acgcttgatc cgaccggagg
     2761 gcgtgcgcga gacgtggact agagggtcgt gggcgtggcg cgcgagtcgt cggtgttcag
     2821 gcagtcggga aaccccgcct gccactcgcc ttgcaacacc tgaatccagc cgtcgaacag
     2881 ggtgtcgaac agatgttcga cctcggcgag cgcctcaacc ggcacccgtt gactcgcgct
     2941 ggtcaggcga tgggacatgt accgatagag cgacaacaga tcgaccaccg cttcctggtc
     3001 ttcccgggag gccaacgcgt tcgacaactc cgccagaatc gccaggacgg tgtgcacgtg
     3061 ccgatcagcg ccgtgcggcg acgtccgggc gcgacgtaac ctcgtcaacg cctccgagta
     3121 gagcagcacg atcatccggt ccgaggcgcc gaccgcgtgg tcggtgggac ggctcggcgc
     3181 ccaccacgcc ggcaacgtcg agtgcggcgg gaaggaacgc tcgttcacgc cgcgagctca
     3241 aacgaacgtt tgggtgacaa caggtcgtcc catcgcgcat cgaccgggta tcgaccgtct
     3301 tgaaacacga cttgccgtcc ccgccgcgtc gccggattga cgatgaccgg cgcggccaga
     3361 ttgagcgcca ccgagtccaa cgtcggcccc gaaatcgtcg cgaagaccgc cacctgacac
     3421 gcccgggcgt cttccagccc cagatcggcc acgtcagcct ccaacccctt gagatcatag
     3481 tcgggcagga tcgacgacgg gtcgaccgcc acgagcgcgg tgtcccgatc gtcaaccgac
     3541 tgccaccacg aaaagcagcc ggcccgttcg tgcccgagcg cgatgaaacg ggtcaacagc
     3601 ggcaagccga tgaggccttt cggcaacacg atgatggccc cgtcgtcgat ttccacggtc
     3661 ccaaaacgcc tcgtctcgat cttcacggct ttttcccctc gtctcccgcg cggtttctcc
     3721 actctcgcca caacgaggac aacacgccgg acggctcggg aacctgagac gcccgccgat
     3781 tctcctcttg aatccgctga taaatctcct cccgatacac gcccgtgctc ctcggcgctt
     3841 cgatgcccaa cttgacttga tgacccttga cttcgagcac gcggacgcgg atatcgtcgc
     3901 cgatcatgat gccttcttcc actcgcctgg ttaagaccaa cacggttgtc cctccttggc
     3961 tatccagcgc gccgccagcg ttgccggctc atcccacagt cttcaaccat gtatgccctc
     4021 gacccccttg atgacttgtt tgaatggttc gatcagcaca gggtcaaacg cgcgtcccgc
     4081 ctcgcgttcg atgtgcgtta tcgcctgccc cgcactaatc gactcccgat agggacgatg
     4141 ggagcggaga gcttcatacg cgtcaacgag gcggagaatg cgggaaagcg ccggaatcga
     4201 tgacccggac aacctatctg gatagcccga accatcaaag ttttcatggt gtgaccgcag
     4261 taactgcaca acggtttcca taccgggcac accggacaat aaatgtcctg gaaccacggc
     4321 gtgctgctgg accgcacgcg tctcttcagg gtccagagca ccgctcttca ggagaattct
     4381 gatgtcgacg gccaggttgc cgacgtcatg taaccgcgcg gcaaggtcga gatgcgccaa
     4441 ctcgtcgtcg gtcaacccga ggtgctctcc ggtcgctcga caaatcgcct ggacacgatc
     4501 ggactgacga acttccttgg gactattcag cttggggagc gagaccagca tttcaatcgt
     4561 cgtgcggaag atctcccgtt gacgatcctg ttccaccgcc aaggatttcg tcaacgtccg
     4621 caattcttcg ttctgccggg tcagggtttc ctggagttcg cgattggtca ctgcgagtcg
     4681 gtagagttcg aacccggatt taatctccaa gcgtatctgt tcatcgttcc acggtttggt
     4741 cagatactta tgaatccccc cgtcgttcac cgcagataag atggcgtgtg tctccgcgta
     4801 gccgctcagg atgattcgaa tggcactcgg cgcgacctcg cgggcgcgac gtaacagttg
     4861 gacgccgtcc atctcgggca ttcgatagtc agagatgatg accccgacag actgctcttg
     4921 caggatcgcg aggccgcgac tcggggcatt ggccgtgatc acttcgtacc cgtctcggcg
     4981 caatagacgc gtcaacgcgc tcaacacgaa cggttcatca tccacgatta agaggcggtt
     5041 cgacatgcct ccgacctcag gccgcctcct cgcctgaatc gtccagcacg atcgaaccat
     5101 cggcagacca ctcaaccgtc gtgatcccgg gatactgctt ggcgagcgac cgcaacagcg
     5161 tttcctgctc ttcaagacgc cccaccagct gccgattttt cgacagaagc ctggcttggt
     5221 ccaacgcgag tcgaacagac agacacaggt cttcattgtc ccacggcttt aacacgaacc
     5281 tgtagacgtg gccctcattg attgccgcga ctacagtatc cagatccgct tttcccgtaa
     5341 tgatcattcg taccacgtga ggataccgtt gcttcagtgt catcaagaga tccagccccg
     5401 ttccatcagg catggcatag tccgtaatga ccagatcaac ctggccggcc gccatcatct
     5461 tgatcgcgtc ccgaacaccc tcggcctcca acacccgata ccgctcgcgg gcaaacaacc
     5521 gacagagaga tcgtcgaaca gacggctcgt cgtccactac cagtatcgtt ggcgcatcgc
     5581 tcatgaccgt cccactccct tccttcgtcc gctggtcgtc aggcggccgc tttcgccgcc
     5641 cgttcgatcg ggaacgatag atggaaggtc gtcccgtcgc cgactcgact ctcgacgcga
     5701 accgcccctc catgacgttc gacaattcca tacacaatac tcagccccaa ccccgttcct
     5761 tgtcccacgg gcttcgttgt aaaaaacgga tcgaaaatct ttgagagatg ctccgtcgga
     5821 attcctgcgc ccgtgtcacg aacatcgacg tacacggtat ccgtatcagc ccatgttttg
     5881 acccaaatct ctccccgctc cggaatcgcc tgcgccgcgt tgaccaacaa attcatgaaa
     5941 acttgattta actggtgggg atagcaccag accttaggga ggtcgccgta gtcgcggtgc
     6001 agcgtcgcct tatatttcaa ttcgttccag acaatgttaa tcgtgctttc caatccctca
     6061 ttcaaatcag accacgccgg cttatcctga tccgcatggg aaaacccccg gagcgcggac
     6121 acgatctttt tgacccgatt cagtccctcg accgattcca ccaccgactg tcgagcatcc
     6181 tcaatcgtct gcgccagctg agtgttttcc ttccaggaca cgatctcctg aatctgcccg
     6241 accaccgagt cgtggagccc ggcttcggtt aatcgcgcga gtagcgcgct ggttcgctcc
     6301 acgtagccag taatgtcact cagatacgtt tgcagcattt gtagattgct attgacgaat
     6361 cccaccggat tgttgatctc gtgggcaacc ccggccgcga gttggcccag ggaggccatc
     6421 ttttcttgtc ggatcagttg atcgtcttta cgcgtcaact ccacgtgcgc cctctcgcgc
     6481 tcgcggagcg cgtcttcgat ttctagcgtt cgttgtttca cgcgaccttc gagcgtcgcc
     6541 tt
//
LOCUS       DBNGTT010000531         3359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2680378, whole genome shotgun sequence.
ACCESSION   DBNGTT010000531 DBNGTT010000000
VERSION     DBNGTT010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2680378"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="AC16BC_12310"
     CDS             <1..282
                     /locus_tag="AC16BC_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3242442.1"
                     /translation="EALEAVARDYGVAIHPGDTRRNIVTRHVALNHLVGREFSVGRVR
                     LRGLMLCEPCKHLAQLTATELSNALVHRGGLRCQILSEGVMRVGDEISP"
     gene            279..728
                     /locus_tag="AC16BC_12315"
     CDS             279..728
                     /locus_tag="AC16BC_12315"
                     /inference="COORDINATES: protein motif:HMM:NF028108.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HIR3242443.1"
                     /translation="MTAPLFPRRSPRETLGGYVILPRLIEKVRLHAQDRLPAEYHVQL
                     LGPELTLDGRFLAFTGVDREALRAAILAAPDDAAVLAWVERHATAHTDEEKRAWAEAI
                     DAYRPDPERAARRAKAYPHVAARVDVGAISVFDLIDLDEGRIDRPTR"
     gene            697..1296
                     /gene="sigZ"
                     /locus_tag="AC16BC_12320"
     CDS             697..1296
                     /gene="sigZ"
                     /locus_tag="AC16BC_12320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigZ"
                     /protein_id="HIR3242444.1"
                     /translation="MKDASTGPRADPPSTDAVWTASADPVRQFIRRRVRDDADADDIL
                     QIVFTKIHAGLGTLADRERLPAWIFRIARNAVIDHIRSRESGPVAVELPEDLADVQEP
                     VSAVAELAGCVRPLIDALPEPYRQVLILTEIEGRTQQELATLLGLSLSGAKSRVQRGR
                     EHLKASLLACCHVERDGRGRVVDVEPRPECSVCSLQSRT"
     gene            1385..1900
                     /locus_tag="AC16BC_12325"
     CDS             1385..1900
                     /locus_tag="AC16BC_12325"
                     /inference="COORDINATES: protein motif:HMM:NF022321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HIR3242445.1"
                     /translation="MNLTTPTRRLTVEFLYLDRSRCTRCQATDTVLDDALAELAGDLR
                     AASMVADVRKIHVKTADQARELGFVTSPTIRLNGHDIAPDIIESACASCSCGCEEEGV
                     ACRAWSYQGQQYDAPPKALIVDAIRRMIADPDRWAGETVPPLRDTPENLKRFFAAQRG
                     TPTPSTDGTCC"
     gene            2062..3276
                     /locus_tag="AC16BC_12330"
     CDS             2062..3276
                     /locus_tag="AC16BC_12330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR3242446.1"
                     /translation="MEWTDGRTGQGRRPTNVLITTTPKQILFTKRLQIFIAATGTLCS
                     ALGLTVMIGWHTHALVVVQVRPDFAAMRYNTALSFLFLGLGLFAAARGRVRWTLVCGI
                     WPTAIGVGTVVEYLAGTNLGIDQLVFTHYMAAGVSHVGRMGLNTAVCYALSGCALLTL
                     GRPIAFRLRPLFVGTCGLVTLGVAASVFFAYLTNIVMGSRWGITTPMAIHTAPGMSLL
                     GVGLLVTARRDHVTNTQDITRFLSGRLVPPAIFALSLVVTMWAWYVSGQHSAERARTR
                     FDEAIQQAHSAILNRMKDYEQVLISSRALFAALRDGDTVARRGGDEFTIILIDMAQID
                     DVALVAQKILNVMAQPFDLHGHQMTVTFSIGIACCPVDGTDAATLLKHADTALYRAKE
                     RGRNNFQIYSAA"
BASE COUNT          564 a         1132 c         1077 g          586 t
ORIGIN      
        1 gaagcgctgg aggccgtggc gcgcgactac ggcgtggcca tccaccccgg cgacacgcgg
       61 cgcaatatcg tgacgcggca cgtggcgctc aaccatctgg tgggacgcga gttctccgtg
      121 ggtcgggtcc gattgcgcgg tttgatgctc tgcgagccgt gtaagcacct ggcccaattg
      181 accgcgaccg agttgtccaa cgcgctcgtg caccgcggcg gcctgcgttg ccagatcctg
      241 agcgagggcg tcatgcgcgt gggcgacgag atctcgccat gaccgcgccc ctcttccccc
      301 gtcgctcccc gcgtgaaacc ctcggcgggt acgtgatcct gccccggttg atcgagaagg
      361 ttcggctgca cgcgcaggac cggttgccgg ccgagtacca tgtgcagctg ctcggaccgg
      421 aactcacgct ggacggacga ttcctcgcgt tcacgggtgt ggaccgcgag gccctgcgcg
      481 ccgcgatcct cgccgcgccc gacgacgccg cggtgctcgc gtgggtggaa cgccatgcca
      541 cggcgcatac cgacgaggag aagcgcgcct gggccgaggc catcgacgcg taccggccgg
      601 accccgaacg ggcggcgcga cgcgccaagg cataccccca cgtggccgcg cgcgtggacg
      661 tgggcgccat cagcgtgttc gatctcatcg atctcgatga aggacgcatc gaccggccca
      721 cgcgctgatc ccccctccac ggacgcggtc tggaccgcgt ccgccgaccc ggtgcgccaa
      781 ttcatccgcc gtcgcgtgcg cgacgacgcg gacgcggacg acatcctgca gatcgtgttt
      841 accaagatcc atgcgggctt gggcacgctc gcggacaggg agcggctgcc cgcgtggatt
      901 ttccggatcg cccgcaacgc ggtgatcgac catatccgaa gccgcgaatc cgggccggtg
      961 gcggtcgaac ttccggagga cttggccgat gtgcaggaac ccgtatccgc ggtcgcggag
     1021 ctggccggtt gcgtgcggcc gctgattgac gccctgcccg agccgtatcg ccaggtcctc
     1081 atcctgaccg aaatcgaggg ccgcacccag caggaactcg ccacgttgct cggcttgtcg
     1141 ctttccggcg ccaagtcccg ggtgcaacgc ggccgcgagc acctcaaggc ctcgctgctg
     1201 gcctgctgcc acgtggagcg cgacggtcgg ggaagggtgg tcgacgtgga gccccggccc
     1261 gagtgttcgg tctgttcgtt acagtctcgg acctgactgc gtcctttttg gcggtgctcc
     1321 gtctgaacag gtgaacggct cgaatggtcg aagccgtcgc cttgtcgatg aaaaggagag
     1381 tgccatgaat ctcaccaccc cgacccgacg gctcaccgtc gaatttctct atttggaccg
     1441 gtcgcgctgc acccgctgcc aagccaccga cacggtgctg gacgatgccc tcgccgaact
     1501 cgcgggtgat ctgcgcgccg cgtcgatggt cgccgatgtc cgcaaaatcc acgtcaagac
     1561 cgcggatcag gcgcgtgaac tgggattcgt cacctcgccc acgatccggc tcaacggcca
     1621 tgacattgcc ccggacatca tcgagagcgc ctgcgcgtcg tgtagttgcg ggtgcgagga
     1681 ggaaggcgtg gcgtgccgcg cgtggtccta tcagggccag cagtacgacg cgccgcccaa
     1741 agcgttgatc gttgacgcga tccggcggat gatcgctgat ccggaccgct gggccggaga
     1801 gacggttccg ccgttgcggg acacgccaga gaacctcaaa cggtttttcg ccgcccaacg
     1861 agggacaccc actccctcca cggacgggac gtgctgctga caaaccgggg gcggtcgaac
     1921 agggttcccg cggaatggac gactcccgaa tccttcctcg gaacgaaccc tccccggagc
     1981 ccgtcgccta ttccgatcag acacgctatt actctgcact tgccggtgaa cgccctcacg
     2041 atatactgaa tcacgccgga catggagtgg acagacggtc gcaccggaca aggccgccgc
     2101 cccaccaacg tcttgatcac gacgacaccc aaacaaattc tatttacgaa gcggcttcag
     2161 attttcatcg ccgccacggg aaccctgtgc agtgcactgg gtttgacggt catgatcggg
     2221 tggcatacac acgccctcgt cgtggtccag gtccggccgg acttcgccgc catgcggtac
     2281 aacaccgcgc tcagctttct gtttctcgga ttgggcctct tcgccgcggc gcgcggtcga
     2341 gtgcggtgga cccttgtctg cggtatctgg ccgacggcca tcggcgtggg gaccgtagtc
     2401 gagtatctgg ccgggacgaa cctcggtatc gatcaactcg tgttcaccca ctatatggcg
     2461 gcgggcgtgt ctcacgtagg ccggatgggg ctgaacaccg cggtgtgtta tgccctatca
     2521 gggtgcgcgc tgctgaccct ggggcggccc atcgcgtttc gactccgccc gctgttcgtc
     2581 gggacgtgtg ggctggtgac cctcggcgtg gcggcgtcgg tgttcttcgc gtacctcacc
     2641 aatatcgtga tgggctctcg atggggcatc accaccccga tggccatcca caccgcgccg
     2701 ggcatgagcc tcctgggcgt cggcctgctg gtgaccgccc ggcgcgacca cgtgaccaac
     2761 acgcaggaca tcacccgatt tctcagcgga cggctcgtcc ccccggcgat cttcgccctg
     2821 tccctcgtcg tcaccatgtg ggcgtggtac gtctccggtc aacacagcgc cgaacgggcg
     2881 cgtactcgct tcgacgaggc gattcagcaa gcgcactcgg cgatcctcaa tcggatgaaa
     2941 gactatgaac aggtcctgat cagcagccgc gcattgttcg ccgcactccg ggacggcgac
     3001 accgtggccc gtcggggcgg cgacgaattc acgatcattc tcatcgacat ggcgcagatc
     3061 gatgacgtgg cgttggtcgc gcaaaaaatc ttgaacgtca tggcgcagcc gtttgattta
     3121 cacgggcacc agatgaccgt tacgttcagc atcgggatcg cctgttgccc ggtggacgga
     3181 acggacgcgg cgacgctcct caaacacgcg gataccgcac tctatcgcgc caaggagcgc
     3241 gggcgcaaca acttccagat ctactccgcc gcgtaaggca acgccgacgg ccccgagtta
     3301 ttcgtgcggg gagcgcgcgt ctttgacgcg tcgatacaac gcgtaccccg tggcggcaa
//
LOCUS       DBNGTT010000532         4851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1787310, whole genome shotgun sequence.
ACCESSION   DBNGTT010000532 DBNGTT010000000
VERSION     DBNGTT010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1787310"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="AC16BC_12335"
     CDS             complement(<1..187)
                     /locus_tag="AC16BC_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HIR3242447.1"
                     /translation="MKIHHLTVDEAVASLRSGPQGLAAEDAGRRLVEFGPNQVERVAR
                     RPLIVRFAKGLTHFFALI"
     gene            complement(213..1889)
                     /locus_tag="AC16BC_12340"
     CDS             complement(213..1889)
                     /locus_tag="AC16BC_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3242448.1"
                     /translation="MVAAACGYVARWLRQPLVVAFVVVGILVGPAALNWVGAADQVDL
                     LAKIGITLLLFLVGLKLDVRVIRSQGAAALAAGLGQVVFTSLVGYGIALGLGMTPVTA
                     LYVAVALTFSSTVIIVKLLSDKREIDALHGRLALGILIVQDMVAVLLMIALSASGAGA
                     QGGPAGDGMLAVIARGVAFLAVVALLMRYVLPRLLDHLAVSQELLVLFAIAWGVSLAA
                     LGDGLGFSQEVGAFVAGVALASTPYREAIGSRLVSLRDFLLLFFFVSLGAQQDLHALG
                     AQAGPAVVFSLFVLIGNPLIVMIVLGAMGYRKRTGFLTGMTVAQISEFSLILASFGRE
                     LGHIDSQTVGLITLVGLVTIALSTYLILYSHVLYERVAPWLGIFERRVPHQEEELDTE
                     NARRAEVIVFGLGRYGSRIVAGLRRRGAAVLGVDFDPEAVARCKSLGVPTHYGDAEDP
                     EFPAGLPLGRARWVVSTTPAREVNLSLLHALRHHGYAGRVALTAHFAQDRDALERSGA
                     DLVLLPYADAADQAVDLLTEPGVRSERAAADRGDAPSAADPNAEIDASRA"
     gene            complement(1968..2810)
                     /locus_tag="AC16BC_12345"
     CDS             complement(1968..2810)
                     /locus_tag="AC16BC_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3242449.1"
                     /translation="MKILLAVDGSRYSRWATETLLSVPWVKRPDVVVLHVVDVGTIPE
                     VLVAPQAAEAYRAVVAADTASRERTGKRLVARVAARLKTRCRTVTAVVANGRAADVIV
                     DRARREAADLIVVGARGVGNVRRFLLGSVSARVVSHAPCSVLVVKRGGRAVRRVVLGA
                     DGSKASERAARRLAAWVAPGAVTVGAVYVWEYPVHPHPAGAVERMVDERYCRALKRAG
                     IACRPQWVMGHPAERLVAIAARKRADLLVVGARGLSAVRRFLLGGVSNHIATHSHGSV
                     LVVR"
     gene            complement(2881..3915)
                     /locus_tag="AC16BC_12350"
     CDS             complement(2881..3915)
                     /locus_tag="AC16BC_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008273 - calcium, potassium:sodium
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006874 - intracellular calcium ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR3242450.1"
                     /translation="MPAVSGVFQPAVVWLEFALCALLIGVAGTFLSRYGDVIAEKTGL
                     SGTWIGVVLLATVTSLPELLTGVSAVAFAHVPNIAIGDALGSCVFNLLLITLLDFVSR
                     GESVYRRASQGHILSAGFGVMLISFVGLNVLLARHDDALRIGHIGLYSPMIVLLYGVA
                     MRTVFRYEREHMASFTEVRAARYPDMTLRRAAARYGGAAAVVIGAGAWLPFVGRDLAD
                     VMGWHNTFVGTLFVAFATSVPEIVVSVAALRIDAPDMAIGNLFGSNLFDVLVIAVDDL
                     FFFKGPILSFVSPVHAVSVVSAVMMTGLAIVGLLYRPDTRLFKAVGWTSLGLLAFYVM
                     NSYVLFLYGE"
     gene            complement(3921..>4851)
                     /gene="hypE"
                     /locus_tag="AC16BC_12355"
     CDS             complement(3921..>4851)
                     /gene="hypE"
                     /locus_tag="AC16BC_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIR3242451.1"
                     /translation="GFGNPQGEARHDAAVAEINGARLALTTDSYVVSPLFFPGGDIGS
                     LAVNGTVNDLAMAGARPLYLSAGFIIEEGLPRDVLTKVVGSMRAAADEAGVRIVTGDT
                     KVVDKGKADGLFINTTGVGLVEARRPIQPNRVRPGDAILVSGDLGRHGIAVMAAREGL
                     AFETALESDSAPLASLVLSLLEAGIDLHCLRDLTRGGLASALNEIAESAGAAIEVDER
                     RISVREDVRGACELLGLDPLYVANEGRLVAFVPDRDAERALALMRAHRFGAGATVIGR
                     VDAAAEPAVTVRSALGTSRVLDMLSGEQLPRIC"
BASE COUNT          933 a         1829 c         1470 g          619 t
ORIGIN      
        1 gaatcagcgc gaaaaagtgg gtgaggccct tggcgaaccg gacgatcagg gggcgccgcg
       61 ccacccgttc gacttgattg gggccgaact cgacgagccg acggcccgcg tcctccgcgg
      121 cgagcccttg gggaccgctg cggaggctcg cgaccgcctc gtcaaccgtg aggtggtgga
      181 ttttcaccgg ctgccctcgg cggaatgcgg cgtcacgcgc ggctcgcgtc gatctccgcg
      241 ttcgggtccg cggctgaggg cgcgtcgccg cgatcggccg cggcgcgctc cgacctcacc
      301 ccgggctccg tcaacagatc cacggcctga tccgccgcgt ccgcgtacgg caagagcacc
      361 aggtccgcgc cgctccgttc caacgcgtcc cgatcctgcg caaagtgggc ggtgagcgcc
      421 acccgtccgg cgtagccgtg atggcgaagc gcgtgcagaa gggaaagatt gacctcccgc
      481 gccggcgtgg tgctgaccac ccagcgcgcc cggcccaacg ggaggcccgc cgggaactcc
      541 ggatcctcgg cgtcgccgta gtgcgtggga acgcccagac tcttgcagcg cgccaccgcc
      601 tcggggtcga aatccacgcc cagcaccgcc gccccccgcc gccgaaggcc cgccacgatg
      661 cggcttccgt accgcccgag accgaacacg atgacctccg cgcgcctggc gttctcggtg
      721 tcgagttcct cctcctgatg cggcacgcgc cgttcaaaga tcccgagcca cggcgccacg
      781 cgttcgtaga gcacgtggga atacaggatc aggtacgtgg acaaggcgat cgtgaccaac
      841 ccgaccaacg tgatcaaccc gacggtctgg gaatcgatgt ggcccagttc ccggccgaac
      901 gacgccagga tcaacgagaa ctcgctgatc tgagccaccg tcatcccggt caagaacccg
      961 gtgcgtttgc ggtagcccat cgcccccaac acgatcatca cgatcagcgg gttcccgatc
     1021 aacacgaaga gcgagaacac caccgcgggt ccggcctgcg cgcccagcgc gtgcaggtcc
     1081 tgctgcgcac ccaggctcac gaagaagaac agcaggagga aatcgcgcag actcacgagg
     1141 cgcgagccga tggcctcgcg atacggagtc gacgccaggg cgaccccggc cacgaacgcg
     1201 ccgacctcct ggctgaatcc caggccgtcc cccaacgccg ccagcgacac gccccacgcg
     1261 atcgcgaaga gcaccagcag ttcctgcgag accgcgaggt gatcgagcag ccgaggcagc
     1321 acgtaccgca tcaatagggc gacgacggcg agaaacgcaa cgccccgcgc aatcaccgcg
     1381 agcatcccgt cgccggccgg gccgccctgc gcgcccgcgc cggacgcgga caacgcgatc
     1441 atgagcagca ccgcgaccat gtcctgcacg atcagaatcc ccagggcgag ccggccgtgc
     1501 agggcgtcga tctcgcgttt gtccgacagg agcttgacga tgatgaccgt gctggagaac
     1561 gtcaacgcca ccgccacgta cagcgcggtc accggcgtca tgccgagccc cagggcgatg
     1621 ccgtacccca cgagggacgt gaacacgacc tgtcccagcc ccgcggccag cgcggcggcc
     1681 ccctgactgc ggatgacacg cacgtccagt ttgaggccga ccagaaagag caggagcgtg
     1741 atcccgatct tggcgagcag gtccacctgg tcggcggccc ccacccagtt caacgcggcc
     1801 gggccgacca ggatccccac caccacgaac gccaccacca acggctgccg caaccaccgc
     1861 gcgacgtacc cgcacgccgc cgccaccatc agcagggcgg cgacctcggc aaacgcgcta
     1921 gggaacactc ggcgccccga gccgaggacc gacggatcga gcgacaatca cctcacgacc
     1981 aaaaccgacc cgtgactgtg ggtcgcgatg tgattcgaca cgccgccaag caggaatcgc
     2041 ctaaccgcgc tcagccccct tgcgccaacc accagcagat ccgcccgctt gcgcgccgcg
     2101 atcgccacca accgttccgc cgggtgcccc atgacccact gcggtcggca ggcgatgccc
     2161 gcgcgcttca aggcgcgaca gtagcgttcg tcgaccatcc gctccaccgc gccggcggga
     2221 tggggatgaa ccggatattc ccagacgtac acggcgccga cggtgacggc ccctggagca
     2281 acccacgcgg cgaggcggcg cgcggcgcgc tccgaggcct tcgacccgtc ggccccgagc
     2341 acgacccggc gcacggcgcg gccgccccgt ttgacgacca gcacggaaca cggcgcatga
     2401 ctcaccacgc gggccgacac gcttcccagc aagaaccggc ggacgttgcc gactccgcgg
     2461 gcccctacca cgatcagatc cgcggcctct cgcctggcgc ggtccacgat gacgtccgcc
     2521 gcgcggccgt tcgccaccac cgccgtgacg gtcctacacc gcgttttcag ccgcgccgcg
     2581 accctcgcga ccaggcgctt tccggtccgc tcccgcgacg ccgtgtccgc ggcgacgacc
     2641 gcgcggtagg cctccgccgc ctgcggcgcg accaacacct caggaatcgt gccgacgtcg
     2701 acgacgtgga gcacgacgac gtccgggcgt ttcacccacg gaacgctcaa caacgtctcc
     2761 gtcgcccagc gggaataccg ggatccgtca acggccagca gaatcttcat catgatgact
     2821 cctcgttatc cacaccacct ctgggtgcgc gctggttcat cacgcgcgac gagacgacca
     2881 ctattcgccg tacaagaata gaacatagga attcatcacg tagaatgcca gcaatccgag
     2941 gctcgtccag ccgaccgcct taaagagcct ggtgtcggga cgatacagca acccgacgat
     3001 cgccagcccg gtcatcatca cggcggagac cacggacacc gcgtgcacgg gcgacacgaa
     3061 tgagaggatc ggccctttga aaaaaaacag gtcatccacc gcgatgacca acacgtcgaa
     3121 caagttgctg ccgaacaaat tcccgatggc catgtccggc gcatcgatgc gcaacgcggc
     3181 cactgaaacc acgatctccg gaacggacgt ggcgaacgcc acgaacaagg tcccgacaaa
     3241 cgtgttgtgc catcccatga cgtccgcgag atctctgcca acgaatggga gccacgcgcc
     3301 ggcgccgatg accacggcag cggccccgcc gtaccgcgcc gcggcccgtc gcaaggtcat
     3361 gtcggggtac cgcgcagcgc gcacctcggt gaacgacgcc atatgctcac gctcgtatcg
     3421 gaacacggtc cgcatcgcga ccccatacaa caagacgatc atcggactgt ataacccgat
     3481 atgtccgatc cgcagcgcgt cgtcatggcg cgcgagcagg acgttgagcc ccacaaaact
     3541 tatcagcatc accccaaagc cggccgacag gatatggccc tgactcgcgc ggcgatagac
     3601 ggactcgccc cggctgacaa agtccagcag cgtaatcagg agcagattaa agacgcaact
     3661 ccccaacgcg tcgccgatcg cgatattcgg gacgtgcgcg aacgccacgg cactgactcc
     3721 cgtgagcaat tccggaagcg atgtcaccgt cgccaacaag acgacgccga tccacgtgcc
     3781 actcaggccc gtcttctccg cgatcacgtc gccatagcgc gacaagaacg tcccggccac
     3841 accgatcagc agggcgcaca gggcaaactc cagccagacc acggccggct ggaacacgcc
     3901 ggaaaccgcc ggcatcgcga tcagcagatc cgcggcaact gttcgccgct gagcatgtcg
     3961 agcacccggg acgtgccgag cgcgctccgc accgtcaccg cgggctccgc cgccgcgtcc
     4021 acgcggccga tgaccgtcgc gccggcgccg aatcggtgcg cccgcatgag ggccagcgcg
     4081 cgctcggcgt cgcgatccgg cacgaacgcg acgaggcgtc cctcattggc cacgtagagc
     4141 ggatcaagcc ccaacaactc gcacgcgccg cgcacgtctt cgcgaaccga gatgcggcgt
     4201 tcgtcgacct cgatggccgc gcccgccgac tccgcgattt cgttcaacgc gctcgcgagc
     4261 cccccgcggg tcaaatcgcg caggcagtgc agatcgatcc cggcctccag caacgacaac
     4321 acgagcgagg ccagcggcgc gctgtcgctc tcgagcgcgg tttcgaacgc gagcccttcg
     4381 cgcgcggcca tgaccgcgat cccgtgtcgt cccagatcgc cgctcaccag gatcgcgtcg
     4441 cccggacgca cccggttcgg ctgaatcgga cgacgagcct ccacgagccc cacgccggtc
     4501 gtgttgatga acaggccgtc ggccttgccc ttgtccacca ccttggtgtc cccggtcacg
     4561 atgcgcacgc ccgcttcatc cgccgcggcg cgcatcgagc cgaccacctt ggtcagcacg
     4621 tcgcgcggca ggccctcttc gatgatgaat cccgcgctca ggtacaaggg cctggccccg
     4681 gccatggcca gatcattgac ggtcccgttc accgccagcg acccgatgtc cccgcccgga
     4741 aagaacaagg ggctgaccac gtacgaatcc gtggtcagcg ccaggcgcgc gccgttgatc
     4801 tcggccacgg ccgcgtcgtg acgagcctcg ccctgcggat tgccgaaccc c
//
LOCUS       DBNGTT010000533         2673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_1404241, whole genome shotgun sequence.
ACCESSION   DBNGTT010000533 DBNGTT010000000
VERSION     DBNGTT010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_1404241"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /gene="radC"
                     /locus_tag="AC16BC_12360"
     CDS             <1..556
                     /gene="radC"
                     /locus_tag="AC16BC_12360"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3242452.1"
                     /translation="ELAARILSEFGDLRAVAQASTTELCRIDGVGPAKAAQLKAAHEL
                     GRRVAAAPLRIGTPIESSAAVFEHFGPLLKGLKQERFVGLYLDGKHRVVLERTISEGS
                     LTASLVHPREAFAPAVRASAAAVIFLHNHPSGDEAPSREDRELTARLASCGRLLGIPM
                     LDHLVIGAERYFSFADHRLVEPEA"
     gene            553..822
                     /locus_tag="AC16BC_12365"
     CDS             553..822
                     /locus_tag="AC16BC_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242453.1"
                     /translation="MTDRRQGDRRRTDRRGAAERRLRRRRSGCDRRVAVRAIAFPDRR
                     MVMRRGSDRRCVDRRRFEAAGIGAAGLAAAASAVGPSGRTEAVAR"
     gene            900..1139
                     /locus_tag="AC16BC_12370"
     CDS             900..1139
                     /locus_tag="AC16BC_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242454.1"
                     /translation="MTDATRRATAVAAELQALRAEMERAAVQGKEVVQKLERLTLAVG
                     ELREAVADLQAALAESDVEYGTVDESDSEMGEGHA"
     gene            1182..>2673
                     /gene="bamA"
                     /locus_tag="AC16BC_12375"
     CDS             1182..>2673
                     /gene="bamA"
                     /locus_tag="AC16BC_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR3242455.1"
                     /translation="MTGRLGFGGRTAVALSVWVLLAIGTAHAASEAPNILSLSVRGAS
                     KIEASTIISRSTLAVGGAADAAAIRDAIKAIYRMGYFDDVSIEQEPGPDGVHVVIVVS
                     ERPILSEVRYEGYAQIGEEKLKEQVSLTPQTAVDLRAVNAAATKIQQYYQSEGYADAR
                     VIPVTKTVSPGSVSVTFYIEEGQRAKIRTVAFDGNTHVPTKRLRKVVSTKPYFWLTSW
                     ASSRGIFKRDDLDQDAERLRDFYLNEGYVQVQVGTPRVTPLPNGKGLTVTFPVEEGEQ
                     FTVARIKLSGHAVFSSSELSEGLVLHEGDVFRRNLLRQDMAMLTDRYGEKGYAFVDIR
                     PQFVPRPESRSIDLTYEIREGRPVTVRTVTVEGNEKTYDRVIRRELRVAEQELVNTKA
                     LKRSFQRINNLNFFENVEILPNPIGDDQMDITVKVKEKPTGTFSVGGGYSSVDRVVYQ
                     ANIEQGNLFGRGQLLRARMEKSSRTTSNYSLTFREPYLFDRPVAGTI"
BASE COUNT          468 a          813 c          981 g          411 t
ORIGIN      
        1 ggagttggcg gcgcggatcc tctcggagtt cggcgatctg cgcgcggtgg cccaagccag
       61 cacgacggag ttgtgtcgga tcgacggggt cgggcccgcg aaagcggcgc aactcaaggc
      121 ggcgcacgaa ctggggcggc gggtggcggc ggcgcccctg cggatcggca ccccgatcga
      181 gtcgagcgcc gcggtcttcg agcacttcgg cccgttgctg aagggactca aacaggagcg
      241 atttgtcggc ctgtatttgg acgggaagca ccgcgtggtc ctggagcgca cgatttccga
      301 gggcagcttg accgcgagtc tggtgcatcc tcgcgaagcc tttgctccgg ccgtgcgcgc
      361 ttccgcggcc gcggtcattt tcctgcacaa ccatcccagc ggagacgaag cgccgagccg
      421 ggaggaccgg gaactgaccg cccgcctcgc gtcgtgcggc cggctgttgg ggatcccgat
      481 gctggatcac ctcgtgatcg gcgccgaacg ctacttcagt ttcgccgatc accggctggt
      541 ggagcccgag gcatgaccga tcgtcgtcag ggcgaccgcc ggcgcaccga ccgccgcggc
      601 gcggccgagc gacggctgcg ccggcgccgc agcggatgtg accgccgcgt ggccgtgcgc
      661 gcgatcgcgt ttcccgatcg ccggatggtc atgcggcggg ggtcggatcg acgatgcgtc
      721 gaccgccggc gattcgaggc ggcggggatc ggcgcggccg ggctcgctgc cgcggcgtcg
      781 gcggtcggac cgtcggggcg gacggaggcc gtggcgcggt gaagccgcga ggatcgcgcc
      841 gaggcgccgg acccaagcgg gcggccgcgt cccccgcaag gacgccgcgg aaacgcgccg
      901 tgacggatgc cacgcgacgc gccacggcgg tcgccgccga attgcaggcg ctgcgggcgg
      961 agatggagcg cgccgcggtc cagggcaaag aggtggtcca gaaactggag cgcctgacat
     1021 tggcggtcgg ggagctgcgg gaagcggtgg ccgacctcca ggccgcgctc gcggagagcg
     1081 acgtggaata cgggacggtc gatgaatcgg attccgaaat gggggagggg catgcataac
     1141 cctcgggacc gcaaccgggc gagcgggcgt tccgagggtt gatgacggga cggttggggt
     1201 tcgggggacg gacggccgtc gcgctgtccg tgtgggtgct cctcgcgatc gggaccgcgc
     1261 atgccgcgtc ggaggcgccg aacattctgt cgttgtcggt gcgcggcgcg tcgaaaatcg
     1321 aagcctccac catcatcagt cgcagcacac tcgccgtggg cggcgcggcc gacgcggcgg
     1381 cgatccggga cgcgatcaag gcgatctacc ggatgggtta tttcgacgac gtctcgatcg
     1441 aacaggagcc ggggcccgac ggcgttcacg tcgtgatcgt agtgagcgaa cgaccgatcc
     1501 tcagcgaagt ccgctacgag gggtatgccc agatcggcga agagaagctc aaggaacaag
     1561 tttccctcac gccgcagacc gccgtggatc tgcgggcggt gaacgcggcc gcgaccaaaa
     1621 tccagcagta ctatcagagc gagggctacg cggacgcgcg cgtgatcccg gtgaccaaaa
     1681 cggtgtcgcc ggggtcggtg tccgtgacgt tctatatcga ggaggggcaa cgcgccaaga
     1741 ttcgcacggt cgcgtttgac ggcaatacgc atgtgcccac caagcgcctg cgcaaagtgg
     1801 tgtcgacgaa gccctacttc tggctcacgt cctgggcgag cagccggggc atcttcaagc
     1861 gggatgactt ggaccaggac gcggagcgtt tgcgggactt ctacctcaac gaagggtatg
     1921 tccaggtgca ggtggggacg ccccgggtga ccccgctgcc gaacggcaag gggttgaccg
     1981 tgaccttccc cgtggaagag ggcgaacagt tcacggtggc ccgtatcaaa ctgtccgggc
     2041 acgccgtgtt ttcgtcgtcg gagttgagcg aaggcctggt gctgcacgag ggcgacgtct
     2101 ttcgtcgcaa cctgcttcgg caggacatgg cgatgctcac ggatcgatac ggggagaagg
     2161 gctacgcgtt cgtggacatt cggccgcagt tcgtgccgcg gcccgagtcg cgatccatcg
     2221 acctgacgta cgagatccgg gaaggccgtc cggtgacggt tcgcaccgtc acggtcgaag
     2281 gcaacgagaa aacgtacgat cgggtcatcc ggcgggaact gcgggtggcg gaacaggagt
     2341 tggtcaacac caaggcgctc aagcgcagtt tccagcgcat caacaacctc aattttttcg
     2401 agaacgtgga aatcctcccg aacccgatcg gcgacgacca gatggacatc acggtcaagg
     2461 tcaaggagaa acccaccgga accttcagcg tgggcggcgg gtacagctcg gtggatcgcg
     2521 tcgtgtatca agccaacatc gagcagggca atctgttcgg gcggggccag ttgctgcgcg
     2581 cccgcatgga aaagagcagc cggaccacgt cgaactactc tctgacgttt cgcgagccgt
     2641 atttgtttga ccggccggtg gccgggacca ttg
//
LOCUS       DBNGTT010000534         1593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2425858, whole genome shotgun sequence.
ACCESSION   DBNGTT010000534 DBNGTT010000000
VERSION     DBNGTT010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2425858"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="AC16BC_12380"
     CDS             complement(<1..410)
                     /locus_tag="AC16BC_12380"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3242456.1"
                     /translation="MSERIHPGLSGADPQRLRAFVAEHRARLRRDHELRRCGPDTVAG
                     LSALMDAVIASLYGAVVPGEGVERPGSGCAVVAVGGYGRGELFPASDVDLMFLFDPPA
                     RGEGLVVSKGVLHPLWDLGYTVGHSLRRIRDCLDL"
     gene            486..899
                     /locus_tag="AC16BC_12385"
     CDS             486..899
                     /locus_tag="AC16BC_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242457.1"
                     /translation="MTEPRSPSPSETTPSSCQTCDQITSRFAGAGIRPLRVAIRCRHC
                     GTVHRHQGAVEPLPKTRRTAKAAAAIAREATYERLLAVTSGRKSRPYSISGVFKPNDV
                     IEHPTFGVGVVTHLVAGNKIQVAFKNGDKLLACRR"
     gene            complement(909..1331)
                     /locus_tag="AC16BC_12390"
     CDS             complement(909..1331)
                     /locus_tag="AC16BC_12390"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3242458.1"
                     /translation="MITEKRASRRVTLMGTTLVRPGSGGRAVTAVVTNVSASGAGLRV
                     PEAISPNEAVSVTVAFLDQAGAVTEEKLTGVVAWMAPSPHGFLVGLTWDQTIDADTHP
                     ALAAYVAPPPPSTPTRPQPSKPRRPSGPAKRGAGRARR"
BASE COUNT          279 a          584 c          496 g          234 t
ORIGIN      
        1 agatccaggc agtctcggat ccggcggagg ctgtgaccca cggtgtaccc gagatcccag
       61 agcgggtgca acacgccctt gctcacgacc aggccttcgc cgcgggccgg cggatcaaag
      121 aggaacatga gatccacgtc cgacgccggg aacaactcgc cgcggccgta cccgccaacc
      181 gccaccacgg cgcaccccga ccccggccgc tccacgcctt cgccgggcac cacggctccg
      241 tacaacgatg cgatcaccgc gtccatcagc gcggacaggc cggccaccgt gtcgggcccg
      301 caccggcgca gctcatgatc ccgtcgcagg cgcgcgcggt gctcggccac gaacgcgcgc
      361 aggcgctgcg ggtcggcgcc cgagagcccg ggatggatgc gttcactcat tgacttttgg
      421 cctcgtaatc cggcagtcta ataccctgac gcgttgatgc ggatccagaa aggacactgc
      481 ccagcatgac cgagccgaga tcgccttccc cctccgagac cacgccctcc tcctgccaaa
      541 cctgcgacca gatcacgtct cgattcgccg gcgccggcat tcgaccgctc cgagtggcca
      601 tccgctgtcg ccattgcggc accgttcatc gccatcaggg cgcggtcgaa ccgctcccca
      661 aaacgcgccg taccgcgaaa gccgccgcgg cgatcgcccg cgaagccacg tatgaacggc
      721 tgctggcggt cacgtccggg cggaagagcc gcccctactc catctccgga gtgttcaaac
      781 cgaacgatgt gatcgaacac ccgacatttg gcgtgggtgt ggtcacccat ctcgtcgccg
      841 gcaacaagat ccaggttgcg ttcaagaacg gcgacaagct gttggcctgc cgccgctaag
      901 gcgacgactc agcggcgagc ccggccggcg ccccgcttgg caggacccga cggacgcctc
      961 ggcttggacg gctgcggacg cgtcggggtg gacggcggag gaggcgccac gtacgcggcc
     1021 agcgcgggat gcgtgtcggc gtcgatagtc tgatcccacg tcaatccgac caagaatccg
     1081 tgcggcgacg gcgccatcca cgccaccacc ccggtgagtt tttcctcggt caccgcacca
     1141 gcctgatcaa gaaaggccac ggtgacgctc accgcttcat tcggcgagat agcttccgga
     1201 acccgcaacc cggcgccgga ggcgctgacg ttggtaacca ccgcagtcac ggcgcggccg
     1261 ccggaccccg gcctgacgag cgtcgtgccc atgagcgtca cgcggcgaga cgcccgtttc
     1321 tccgtaatca taccgtcccc ctcgagtgaa tgtgtgttca cgagcgcggc gccccgcatc
     1381 ggactgcgga agccatgcgt gaccgtggcc gcgttcctcc ctgatggtgc ctgagcacgt
     1441 ccgtggcgtc gcgttgcgcc gactctctcg cccacagcgg gctgtgtgag taacgtgcgc
     1501 gggcgcctcc tagcacctcg aagacgtccg accgcgtgcg gcgctcaaca tctggaagcc
     1561 ggatggaccg tgcggtcgcc gaagcataac aca
//
LOCUS       DBNGTT010000535         1508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_510274, whole genome shotgun sequence.
ACCESSION   DBNGTT010000535 DBNGTT010000000
VERSION     DBNGTT010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_510274"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC16BC_12395"
     CDS             complement(<1..223)
                     /locus_tag="AC16BC_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242459.1"
                     /translation="MIADLVNGLIAFVSAVVAGSPLAAASALLALALVVILVRVQQSP
                     ARRSRAGLHREAKALLAQGAYLEAGRAFQA"
     gene            complement(220..>1508)
                     /locus_tag="AC16BC_12400"
     CDS             complement(220..>1508)
                     /locus_tag="AC16BC_12400"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3242460.1"
                     /translation="NKLRPRTPPFHGMLVAQSEAKRFYLAIVDNEPYAAGQEQQGVFT
                     PLSLRGLFGGLRKLPAAASELTLVGTDPLYLKSVLTIIQGVPTTEGTTELLEIGSVVE
                     HIKKNSQDQLLAVGYQGELSLFFFRNGQAVAGYFADGAFDSGEDSLEERLLAFVYKYA
                     GQGELSLHVFAGMSAHWAEDGVFERGAWPDALVEHFTRPVPSLFISGSDGAARRFELT
                     SPSVSLGRGEDNDIVIEDPAISRRHLVFRQDAAGVSVEDCGSRNGTLFNGEPLTKAAL
                     SHGAELQIGECLIRFLGGIASPEPIGDAERGEETICRPSADLSPPAPAPTREPDRAVW
                     GLDVVRPDGEHHRVVLSTPLTTVGRVRADLTLIDTKVSRRHGELVLTPEGVVYRDTGS
                     TNGTILNGRAVTSALLKPGDVLKIGETSLTVFRDAA"
BASE COUNT          293 a          511 c          448 g          256 t
ORIGIN      
        1 gcgcttggaa cgcgcgtccc gcctcgagat acgcgccctg cgccagcaag gccttggctt
       61 cccgatggag tcccgcgcgg ctgcgtcgtg cgggcgactg ctggacgcgc acgaggatga
      121 cgaccaacgc gagcgccagc aacgcgctcg cggcggcgag cggcgacccc gccaccaccg
      181 cggatacaaa ggcgatgagc ccgttgacga gatcagcgat cacgcagcat ctcggaacac
      241 cgtcaaagac gtttccccga tcttcaagac gtcaccgggt ttgaggagcg cgctcgtcac
      301 ggcgcggccg ttcagaatgg tcccgttggt gctgccggtg tcgcgataga cgacgccttc
      361 cggagtcaac accaattcgc cgtgtcgacg ggacaccttg gtatcgatca acgtgagatc
      421 tgccctcaca cggccaaccg tggtcagcgg cgtactcaat accacgcgat ggtgctctcc
      481 gtcggggcgc acgacatcga gcccccacac ggcgcgatcg ggttcgcgcg tcggcgcggg
      541 tgccggaggc gacaggtccg ccgaaggacg gcagatggtc tcttctcccc gttcggcgtc
      601 gccgatcggc tcgggcgacg cgatcccccc caaaaaccgg atcaggcact ctccgatctg
      661 caactccgct ccgtgactaa gcgcggcctt ggtcagcggc tcgccgttga acaacgtgcc
      721 gttgcgactt ccgcaatcct ccacgctcac gcccgcggcg tcctgacgga agaccaggtg
      781 tcggcgagaa atcgccgggt cctcgatcac gatgtcgttg tcctcccctc gccccagact
      841 gaccgaaggc gatgtgagtt cgaagcgtcg cgcggcccca tcggagccgg aaataaacag
      901 ggagggaacc ggacgggtaa agtgctccac taacgcgtcg ggccacgcgc ctcgctcaaa
      961 gactccgtcc tcggcccaat gcgccgacat ccccgcgaac acgtgaagcg acagctcacc
     1021 ctgaccggca tacttgtaga caaaagccag aagacgctct tcgagcgaat cctcccccga
     1081 atcgaaggcg ccgtccgcaa agtaccccgc aaccgcttgg ccgttcctga aaaagaacag
     1141 gcttaactct ccctgatacc cgaccgctaa cagttgatcc tgtgagtttt ttttgatgtg
     1201 ttccacgacg ctgccgattt ccaggagctc cgtggtgcct tcagtggtcg gcacaccctg
     1261 gatgatcgtc aagaccgact tgagatacag cggatcagtc ccgacaaggg tcagctcgct
     1321 cgcggcggcc ggcagcttgc gaagcccccc gaacaatccg cggagcgaca gcggcgtaaa
     1381 gaccccttgc tgttcctgcc ccgccgcgta cggttcattg tcgacgatcg caagatagaa
     1441 acgcttggct tccgattgcg ccaccagcat tccatggaac ggaggggtcc ttgggcgcag
     1501 tttgttga
//
LOCUS       DBNGTT010000536         4371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2554432, whole genome shotgun sequence.
ACCESSION   DBNGTT010000536 DBNGTT010000000
VERSION     DBNGTT010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2554432"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="AC16BC_12405"
     CDS             <1..1538
                     /locus_tag="AC16BC_12405"
                     /inference="COORDINATES: protein motif:HMM:NF048511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HIR3242461.1"
                     /translation="EADVAIVRGRLEVSGPIAAIDLSRHTGIPTSRVDQALLALEHEG
                     FVLRGSFTPSPPTTEWCARRLLARIHRLTLDGLRRQIEPASPETFMRFLLQWTHLAPD
                     AQVRGRHGLRDVFDQLQGFDIPAVAWERDILPARVADYDPQWLDDLCLSGELMWTRVT
                     TPPSATGDGDRPAQRTPGRTVPLSLLARQALPWVGWRAAAKELPQVSPKARTVWDALS
                     RQGASFVSDLASSTRLLPAEVTVALWELAAVGVATQDGFAGVRALVDAKRQHRGRHRQ
                     ARTPVRGMGRWTLLSRPAPSDDHLTDEILEPWARQLLRRYGVVFRELLTREDAAPPWS
                     RLLSVFRRLESRGEIRGGRFVAGVGGEQYALSETIEPLRRLRDASPDPTPVIVSATDP
                     ANLAGIITPGDRVPAQAGSCVAFIAGRAVGSRQGAAFEIAATLDPDTAQKVHHALAKG
                     PTWTMPGDQISAGSWRDELNARRRVVRLRTERQEVATRRLQNPPLPPFFKGGVRERGS
                     KGD"
     gene            1694..2785
                     /locus_tag="AC16BC_12410"
     CDS             1694..2785
                     /locus_tag="AC16BC_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR3242462.1"
                     /translation="MVSYLKRIATGPQMSKNLSREEAHDGMTLVLNRSVDPVRAGVFL
                     VALRMKRETDEENLGVLTALRDTTHAASVDVPDLVDLGDPYDGFARHLPASPFLPAVL
                     AACGVPTVSHGCESLGPKSGVTHHQILKAAGLRVDLTPEEAAARIADPEIGWAYVDQA
                     QFHPELDALSELRRLIIKRPCISTWEKLCGPVRASGRNHLIVGYVHPGYERPITMAGR
                     DVGYASTLVVRGIEGGVIPSLNAQTRVVSYQSGKPDEEWKLNPKDADIESTVRSVPIT
                     VKGDQSATDEAIDDVSQDPDLSRLAAPAAEAGLEALKGKPGPTRDSLVLAAAMILRQV
                     GRADTVQSAADLARRVLDSGDALRRLTCD"
     gene            2818..2991
                     /locus_tag="AC16BC_12415"
     CDS             2818..2991
                     /locus_tag="AC16BC_12415"
                     /inference="COORDINATES: protein motif:HMM:NF040165.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242463.1"
                     /translation="MAEVLRRKEPWERLAIGFALWRDARKMLDYHLRTTHPDWTDEQV
                     SREVTRRMSHGAV"
     gene            2978..3541
                     /locus_tag="AC16BC_12420"
     CDS             2978..3541
                     /locus_tag="AC16BC_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242464.1"
                     /translation="MEPSDLLHIVADVFDRLRIPYLVTGSVASMTYGEPRLTNDIDIV
                     ADIRSEHIRDLLTAFPSPEYYISEDMIRDALARRSQFNIIHPASGLKVDVIIRRDTPF
                     DQSRFARRRRIRPTKDFEADFATPEDVIVKKMEYYKEGGSEKHLRDITGILKISGSDI
                     DRRYVAAWAERLGVTDVWEAILRRLES"
     gene            complement(4070..>4371)
                     /locus_tag="AC16BC_12425"
     CDS             complement(4070..>4371)
                     /locus_tag="AC16BC_12425"
                     /inference="COORDINATES: protein motif:HMM:NF019325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HIR3242465.1"
                     /translation="DPAPGRILVTGEVLPECPYLVGWYLSPDTVITTDDTQIGGFCPN
                     GLEAGQTLRFWADVYVPVNIEGGFYYWGAIADYNNSVVETDETNNAATGAQVTIR"
BASE COUNT          865 a         1451 c         1320 g          735 t
ORIGIN      
        1 gcgaggccga cgtcgccatc gtccgaggcc ggctggaagt ctccggtccg atcgccgcca
       61 tcgatctgtc acgccacact gggattccga cgtcccgagt cgaccaagcc cttctcgccc
      121 ttgaacatga aggttttgtc cttcgcggca gtttcacgcc aagcccgccc accacggagt
      181 ggtgcgcgcg gcgcctgctg gcgcgcattc atcggttgac gctcgacggt ctccgtcggc
      241 agatcgaacc cgcaagtccc gaaacgttca tgcggttcct gcttcagtgg acgcacctcg
      301 cccctgacgc gcaggtacgc ggacgccacg gcctgcgcga cgtgttcgac cagttacagg
      361 gcttcgacat cccggccgtt gcctgggagc gcgacatcct gcccgcgcgc gtggcggact
      421 acgatccgca atggctggac gacctgtgct tgtccggcga actcatgtgg acgcgcgtca
      481 ccacgccccc cagcgcgacc ggcgacggcg accgccccgc ccagcgaacg ccgggccgga
      541 ccgtcccgct ctcgttgctg gcgcgtcagg cgctgccgtg ggtcggctgg cgcgccgctg
      601 caaaagaact tccacaggtc agcccgaagg cccggaccgt gtgggacgcg ctcagccgtc
      661 agggcgcctc gttcgtgtcg gacctcgcgt catccacgcg tctgcttccg gccgaagtca
      721 cggtcgcgct ctgggagttg gccgcggtgg gcgtggccac ccaagacggg ttcgcgggcg
      781 tgcgcgcgtt ggtcgatgcc aaacgccagc accgcggccg ccaccgccag gcgagaacac
      841 ctgtccgagg catggggcgt tggaccctgt tgtcgaggcc ggccccctcc gacgatcacc
      901 tcaccgacga gatcctcgaa ccgtgggcgc gccaacttct gcgacgctac ggcgtggtat
      961 ttcgcgagct gctcacgcgc gaagacgcgg ccccgccctg gtcgcggttg ctctccgtgt
     1021 ttcgcagact ggagtctcgg ggagaaattc gcggcggacg attcgtggcc ggcgtgggcg
     1081 gcgagcaata cgcgctgtcc gagaccatcg agccgctccg ccgactgcgc gacgcctcgc
     1141 ccgaccccac gccggtcatc gtgtccgcga ctgatcccgc gaacctcgcg ggtatcatca
     1201 ctcccggcga tcgtgtgccg gcccaggccg ggtcgtgcgt ggcgttcatc gcgggcagag
     1261 ccgtggggtc aagacaaggc gcggcgttcg agatcgcggc cacgctcgat cccgacacgg
     1321 cccagaaggt ccatcacgcc ttggccaagg gcccgacgtg gacaatgccc ggcgaccaga
     1381 tcagcgcagg atcgtggcgc gacgaactca acgcgcggcg aagggtcgtc agattgcgaa
     1441 ccgagcgaca agaggtggcg acgcgccgcc tacaaaatcc ccccctaccc ccctttttca
     1501 aagggggggt aagagaacgg ggttcgaagg gggactagga ggagcgtgtt taaatggtcg
     1561 gtcgcgtgat tgagaacaat tcacctatta ttcacatccc ccctttaaca aaggggggca
     1621 cggggggatg tgcagtacga atgggcacaa cacgatgagc tcagacatcg cagcatcgtc
     1681 ccccgacgac cggatggtct cctacctgaa gcggatcgcc accggccccc aaatgagcaa
     1741 aaatctttcg cgggaggaag ctcacgacgg catgacgttg gtcctgaacc gctcagtcga
     1801 tcccgtgcga gccggagtct tcctcgtggc attgcggatg aagcgcgaaa ccgacgaaga
     1861 aaacctcggc gtgctcaccg cgctgcgcga caccacacac gccgcgagcg tggacgtgcc
     1921 cgacctggtg gacctcggcg atccctacga cggcttcgcc cgccacctcc ccgcctcgcc
     1981 gtttctccct gccgtgctcg cggcctgcgg cgtgcccacc gtgtcgcacg gctgcgaatc
     2041 gctcggcccc aaatccggcg tgacccatca ccagatcctc aaagccgcgg gcctgcgcgt
     2101 ggacctgacc ccggaagaag ccgccgcgcg catcgccgat cccgagatcg gctgggccta
     2161 tgtggaccag gcgcaattcc accccgaact cgacgcacta tccgaactgc gccgcctgat
     2221 catcaagcgc ccgtgcatct cgacctggga aaaactctgc ggcccggtgc gcgccagcgg
     2281 aagaaatcac ctgatcgtcg gctacgtcca ccccggctac gaacgcccga ttaccatggc
     2341 cggccgcgac gtgggctacg cctccacgct cgtggtccgc ggcatcgaag gcggcgtgat
     2401 cccgtccctc aacgcgcaga cgcgcgtcgt ttcctaccag agcggcaaac cggacgaaga
     2461 atggaaactc aaccccaaag acgcggacat cgagtccacg gtacgatcag tgccaatcac
     2521 agtaaaaggc gatcagagcg caacggacga ggccatagac gacgtatcgc aagaccccga
     2581 cctgtctcgc ctcgcggcac ccgccgctga agcgggcctc gaagcactca agggaaaacc
     2641 aggaccgacg cgcgacagcc tggtgctggc tgcggcgatg atcctccgtc aggtgggacg
     2701 agccgacacg gtgcaatcgg ccgcggattt ggccaggcgc gtcctggact caggtgatgc
     2761 gttgcggaga ctgacatgcg attagacccg ggccagatcg aagtgctgga cgatgcgatg
     2821 gccgaggtct tgcgtcgcaa agagccgtgg gagcgtctcg caatcggatt tgcgctctgg
     2881 cgagacgctc gtaagatgct cgattaccat cttcgtacta cccatcccga ctggaccgat
     2941 gagcaagtta gccgcgaagt aacgagaaga atgtctcatg gagccgtctg acttactgca
     3001 catcgtcgcc gacgtcttcg accggctgcg gattccgtat ctcgtcaccg gctcagtcgc
     3061 gtcaatgacc tacggagagc ccagactgac caacgacatc gacatcgtcg ccgacattcg
     3121 gtccgaacac atcagagacc tcctgaccgc gtttccgtct ccggagtact acatcagcga
     3181 agacatgatc cgcgatgcgc tcgccaggcg cagtcagttc aacattatcc atccggcctc
     3241 gggcctgaaa gtcgacgtga tcattcgtcg cgacaccccc ttcgatcaaa gtcgattcgc
     3301 caggagaagg cggatacggc ccaccaagga tttcgaggcg gacttcgcga cgccggaaga
     3361 tgtgatcgta aagaaaatgg agtattacaa agaaggcgga tccgaaaaac atctcaggga
     3421 tatcactggc atcctaaaga tcagtggatc agacatcgac cggcgctacg tcgcagcatg
     3481 ggcggaacga ctcggcgtca cggacgtctg ggaagcgatc ctgcgacgtc tggagtcgta
     3541 ggcgaattgc tccaccacaa acatgagccc cagacagaac taacgattaa agattcgacc
     3601 ctgcaccgcc ccgtttagcg ataaaccctt tcttggcgcc acggcacgac agacgccctg
     3661 gcctggaggt cagtccgccg aatggtttct ggaagaaaca ctctcggtaa gatgttgcaa
     3721 tgcaagacat ggccccaagc gctttttcga cacgcgatag cctggtgttg gctgcagcga
     3781 tgatcctccg tcaggtggga cgagccgaca cggtgcaatc ggccgcggat ttggccaggc
     3841 gcgccatcga ctcgggtgaa gcgcgaagac ggttcgagtc agcgtcggac ggttggaact
     3901 caaaagccct cttttgacgc catcgccgca ggaagcccag gacgccggct tccgagcgcc
     3961 tatccgcccg gggccgaacg ctcagggtga gccgcccgac tccgggcgca acggaacagc
     4021 ccgcccgagt atccgctgtt gttatagggt agatccctca gtagtctgat tacctgatag
     4081 ttacctgcgc tcccgtagcg gcattatttg tctcgtccgt ttcaaccacg ctgttattgt
     4141 aatcggcaat cgccccccaa taatagaacc cgccttcgat gttcacagga acatatacgt
     4201 cggcccagaa gcggagtgtt tggcccgctt caagtccgtt cggacagaaa cctccgatct
     4261 gcgtgtcatc ggtggttatt accgtatccg gcgataaata ccacccaacc agatagggac
     4321 actcgggcag gacctcgccc gtcaccaaaa tacggcccgg cgcagggtcc g
//
LOCUS       DBNGTT010000537         4427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate MAG05354
            SRR25158456_k127_2682321, whole genome shotgun sequence.
ACCESSION   DBNGTT010000537 DBNGTT010000000
VERSION     DBNGTT010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414762
            Sequence Read Archive: SRR25158456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:55:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,491
            CDSs (total)                      :: 2,465
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158456_k127_2682321"
                     /isolate="MAG05354"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="22.92611111 N 108.3730556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC16BC_12430"
     CDS             complement(<1..393)
                     /locus_tag="AC16BC_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR3242466.1"
                     /translation="MPAFPGWRAALGPGVVWMALAQGSGELIWWPYVVAKYGLTFLAL
                     LVPACLLQYPLTVAIGRYTLLTGETIFQGFFRLSRSLGIVLWALMTLSFLWFGAFASA
                     GGTAMAALTNFPAGWTARGQTLFWAYTTI"
     gene            505..2598
                     /locus_tag="AC16BC_12435"
     CDS             505..2598
                     /locus_tag="AC16BC_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3242467.1"
                     /translation="MSAPSTEPAPVPRHTNRLIHETSPYLLQHAHNPVDWYPWGGDAF
                     ARARSENKPILLSIGYSACHWCHVMEHESFENDDIADLMNRHFINIKVDREERPDLDQ
                     IYQTAFQVFNRRGGGWPLTMFLTPDGKPFHAGTYFPPNDRHGIPGFPRVLEAVATAYR
                     DKADDLTRTGEQAVAILERIDASEPASAVPGRELIGTSVESLTRLFEPTYGGFGTGPK
                     FPSTMTLELFLRHARTSGDADAKRRAAFTLQMMAAGGIYDQLGGGFHRYSVDGQWLVP
                     HFEKMLYDNALLVPLALDLFQATGDADFARVARETVEYLLREMRHPEGGFFSTQDADS
                     EGHEGKFFVWTPEEIERVLGPEESKLFGRAYGVTAGGNFEGKTILHVVLRDDALAVEF
                     NRPVEDVRARLATARRALFEARETRVKPFRDEKVLTSWNGLTIAALARAGAALGEPRY
                     TEAAERAAAFIARALWKEGRLLRTWKDGAAKLNGYLDDYAFLADGLLHLYEATGAPRH
                     LAWARELTDTMLAQFWAKDGRGFHLTSHDHETLVTRPLSGADQSIPSGSSQAVFALLR
                     LAAYQNVTAYRGFAEHVFTLSGRAMEDNPFAYANLLGALDLYHEGTSDVVIIGQADHP
                     ACLALLAAVHAVYLPNRTLTVVDPQRADQSTVPEAARGKTMLNGRPTAYVCRNFTCSA
                     PVTDPEALRTLLSQP"
     gene            2606..2836
                     /locus_tag="AC16BC_12440"
     CDS             2606..2836
                     /locus_tag="AC16BC_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242468.1"
                     /translation="MTLQIVRMNEINQIFAVTDRLGIHREAVEIPLSPESPGRVTRTP
                     SGKFHIVVDADVPLAAWVLTLETELQKLLAAP"
     gene            2833..3132
                     /locus_tag="AC16BC_12445"
     CDS             2833..3132
                     /locus_tag="AC16BC_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR3242469.1"
                     /translation="MSASRTAEAPTRQEQPALGDSVGDRFRVILYNDDVHAFDDVVRQ
                     VQRATGVSTEEAFAITLQAHEAGRAVCYVGSSDACDKVAGILREIALHVEIDHAG"
     gene            complement(3317..4105)
                     /locus_tag="AC16BC_12450"
     CDS             complement(3317..4105)
                     /locus_tag="AC16BC_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3242470.1"
                     /translation="MKFDRRHDPLLEAVREIQEHCEARGLSVFSGYLTREHETVAVWN
                     VARGRDWKAFLDLAASRGAPLVYLDWIVFDDAQVEDAVIRAEEGGEAGEPDPQVEDYN
                     REVESFRAYAGLTGVIEIAFSLGGVFHFFEWQSEWFAEFERLVDAWDEGAYQPRRLAR
                     PRLHELANQLVNHPKYQACQSKSQRRYLLEQLLGREFESLRASDVATILDRAEAIFEL
                     EIKPLERSFLRQRARELKAQGLSLDAIAGSLGVSRAAVKALLAG"
     gene            complement(4197..>4427)
                     /locus_tag="AC16BC_12455"
     CDS             complement(4197..>4427)
                     /locus_tag="AC16BC_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HIR3242471.1"
                     /translation="ARPVDGKRRKELKALFAGCRAGLVFVTAFATRDAMRNFLTLISW
                     ESEVWIAENPDHLIHFDGERFLGPYPDVMPGG"
BASE COUNT          818 a         1514 c         1392 g          703 t
ORIGIN      
        1 gatcgtcgtg tacgcccaaa acaacgtctg cccgcgggcc gtccatcccg cggggaagtt
       61 cgtgagcgcg gccatggcgg ttccgcccgc ggacgcgaac gcgccgaacc acagaaacga
      121 cagggtcatc agcgcccaca ggacaatccc cagtgaacga ctcaggcgga agaacccttg
      181 aaagatcgtc tcgccggtca acaacgtgta ccggccgatc gccacggtga gcgggtattg
      241 cagcaggcac gcgggcacca gcagcgccag gaacgtcagc ccgtacttgg ccaccacgta
      301 gggccaccag atcagttccc cgctgccttg ggcaagcgcc atccacacca cgcccgggcc
      361 gagcgcggcg cgccaacccg ggaacgcggg caacggttta cgcccgggga acgagaagcc
      421 cgtcacggca tcacgcggtc cgggccgtgt ttacacaccg cgacgcgtcg tattaagata
      481 gcgtcccaac ggagatgact cgtcatgtca gcgccttcca cggaaccggc gccggttccc
      541 cggcacacca accgattgat ccacgagacc agcccctact tgctgcaaca cgcgcacaac
      601 ccggtggatt ggtatccctg gggaggcgac gcgttcgcca gggcccgctc cgagaacaaa
      661 ccgattctcc tgtccatcgg ctactcggcc tgtcactggt gtcacgtgat ggaacacgag
      721 tcgttcgaaa acgacgacat cgccgatttg atgaaccggc attttatcaa catcaaggtc
      781 gaccgggaag agcgtcctga cctcgaccag atctaccaga ccgcgttcca ggtctttaat
      841 cgtcgcggcg gcgggtggcc gctgaccatg ttcctgaccc cggacggcaa gccgtttcac
      901 gcgggcacgt atttcccccc gaacgaccgg cacggcatcc cggggttccc gcgcgtactc
      961 gaagccgtgg cgacggccta tcgggacaag gccgacgacc tgacgaggac cggggaacag
     1021 gccgtggcga tccttgagcg gatcgacgcg tcggagcccg cgtccgccgt gccgggtcgc
     1081 gagctgatcg ggacgtcggt ggagtcgctc acccggctgt tcgaacccac gtacggagga
     1141 ttcggcaccg gccccaagtt tcccagcacc atgacgctgg aattgtttct tcgccacgcg
     1201 cgcacgagcg gagacgcgga cgccaaacgc cgcgccgcct ttacgctgca gatgatggcc
     1261 gccggcggca tctacgatca actgggcggc gggtttcacc gctactcggt ggacggccag
     1321 tggctcgtcc cgcacttcga gaaaatgctc tacgacaacg ccctgttggt gcccctcgcg
     1381 ctcgatctgt ttcaggccac gggcgacgcg gacttcgcgc gcgtcgcccg cgagaccgtc
     1441 gagtacctgc tgcgcgagat gcgccacccc gagggcgggt tcttcagcac gcaggacgcg
     1501 gacagcgagg gccacgaagg aaagtttttc gtctggacgc ccgaagagat cgagcgcgtc
     1561 ctgggcccgg aggagagcaa gctgttcggc cgggcgtacg gcgtgacggc cggcgggaac
     1621 ttcgaaggca agaccatcct gcacgtggtg ctgcgcgacg acgcgttggc cgtggagttc
     1681 aaccgtcccg tcgaggacgt gcgcgcgcgc ctcgccacgg cgcgacgcgc gctgttcgag
     1741 gcgcgcgaaa cccgcgtcaa gccgttccgc gatgagaagg tcctcacgag ctggaacggc
     1801 ctgactatcg ccgcgctggc ccgggcgggc gcggcgctcg gcgaaccgcg ctacaccgaa
     1861 gccgcggagc gcgccgcggc gttcatcgcc cgcgcgctgt ggaaggaggg ccgcctgctc
     1921 cgcacctgga aggacggcgc ggccaaactc aacgggtatc tggacgacta cgcgttcctg
     1981 gccgacggct tgctccacct gtacgaagcc acgggtgcgc cgcggcacct ggcgtgggcg
     2041 cgcgagctga ccgacaccat gctcgcccag ttttgggcca aagacgggcg cggatttcat
     2101 ctgaccagcc acgaccacga aaccctggtc acgcggccgc tctcgggcgc ggaccaatcc
     2161 atcccgtcgg gatcgtcgca ggccgtgttc gcgttgttgc gcctggccgc gtatcagaac
     2221 gtgacggcgt accgcgggtt cgccgaacac gtcttcacgt tgtccggccg cgccatggaa
     2281 gacaacccgt tcgcatacgc gaacctgctg ggcgcgttgg acctctacca cgagggcacc
     2341 agcgacgtgg tgatcatcgg gcaagccgac catcccgcgt gcctggcgct gctggcggcg
     2401 gtccacgccg tgtatctgcc gaaccgcacg ctgaccgtcg tggaccctca acgcgccgat
     2461 caatcgacgg tgcccgaagc ggcccgcggc aagaccatgc tcaacggccg cccgaccgcg
     2521 tacgtctgcc ggaacttcac gtgttcggcg cccgtcaccg atcccgaggc cttacgaacc
     2581 ctgttgtccc aaccgtgagt cgaccatgac gctgcagatc gtccggatga atgaaatcaa
     2641 ccagatcttc gcggtcaccg accggctcgg catccaccgc gaggccgtcg aaatcccgct
     2701 gagcccggaa tcgcccggcc gggtgacgcg cacgccctcg ggcaagtttc acatcgtggt
     2761 ggacgcggac gtccccctcg ccgcgtgggt gctgaccctc gaaaccgaac tccagaaatt
     2821 actcgccgcg ccatgagcgc ctcgcgcacc gcggaggctc cgacccggca ggaacaaccg
     2881 gccctcggcg actcggtcgg ggaccgtttc cgcgtgattc tgtacaacga cgacgtgcac
     2941 gcgttcgacg acgtggtcag gcaggttcag cgggccaccg gggtctcgac ggaagaagcg
     3001 ttcgcgatca ccttacaggc ccacgaggcg ggccgggccg tctgttacgt aggctcgtcc
     3061 gacgcctgcg acaaagtggc cggcatcctg cgcgagatcg cgctgcacgt ggaaatcgac
     3121 cacgcggggt gaccgagcgc acggcagcct acgacttgta attaatccct tccacgagtc
     3181 gcgacgacgc gtgagcccgt tcaggaatgc gccagatgca aggcgccgcg agcaccggag
     3241 cgcagcgtac gaggtgggta cgtgagcacc ggctgcgcag cggcaacgca gcagatggcc
     3301 attccgggac gggctcctat ccggcgagca gggctttgac cgcggcacgg gacaccccca
     3361 aactcccggc gatcgcgtcg agcgacaacc cttgcgcctt gagttcgcgt gcccgctgtc
     3421 gcagaaaact tcgttcgagc ggcttgatct ccagttcgaa gatcgcctcg gcgcgatcca
     3481 gaatcgtggc cacgtcagac gcccgcagcg attcgaattc acggccgagg agttgctcca
     3541 aaagataccg ccgctgagac ttgctctgac aggcctggta cttaggatgg ttgaccagtt
     3601 ggttcgccag ctcgtgcagg cggggcctgg ccagtcgccg cggctggtac gcgccttcgt
     3661 cccacgcatc caccaatcgc tcgaactcgg cgaaccattc cgactgccat tcgaagaagt
     3721 gaaacacccc gccgagtgaa aacgcgattt caatgacgcc ggtgagcccc gcgtacgcgc
     3781 ggaaactttc gacctctcgg ttgtagtcct cgacctgcgg gtccggctct ccggcttcgc
     3841 ccccttcttc ggcgcggatc accgcgtctt cgacctgggc gtcgtcgaac acgatccaat
     3901 ccagatagac gagcggcgcc ccgcgcgagg ccgccagatc caagaacgcc ttccagtccc
     3961 gtccgcgcgc gacgttccac accgcgacgg tttcgtgctc gcgggtcagg tacccggaaa
     4021 acacgctcag gcccctggct tcgcagtgtt cttggatttc ccggacggct tccaacaacg
     4081 gatcgtgtcg gcggtcgaat ttcatgggcg agggtcgggt ggcagtggtc tcaggagatg
     4141 gattgtacac gggtcaggat cggtactcaa gaaccccgag cgcatgaatg gaaccgctag
     4201 ccccctggca tcacatccgg atacgggccg agaaaccgct cgccgtcaaa atgaatgagg
     4261 tggtcaggat tttctgcgat ccacacctca ctctcccacg aaatcagcgt gaggaaattc
     4321 cgcatcgcgt cacgcgtcgc aaacgccgtg acgaacacga ggcctgctcg acacccggcg
     4381 aagagggctt ttagttcctt ccgtcgcttg ccgtcgacag gcctggc
//
